LOCUS       DATMKC010000001         5598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_23379, whole genome shotgun sequence.
ACCESSION   DATMKC010000001 DATMKC010000000
VERSION     DATMKC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23379"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VNU01_00005"
     CDS             complement(<1..339)
                     /locus_tag="VNU01_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31032.1"
                     /translation="MRVLVSTQPAPSHFGPLVPYLEALREAGHEVLVACAASFTGAVE
                     DKGFAAVACGLDWLEVEAAKTFPEVSELDGRGKLLFSLRHMFGGVLADAFARDLPAVI
                     EDWGADVLLWE"
     gene            complement(367..738)
                     /locus_tag="VNU01_00010"
     CDS             complement(367..738)
                     /locus_tag="VNU01_00010"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWH31033.1"
                     /translation="MHKDLTLIPDDQPGVLARIGEAAGAAGLNIEGVSAFTGQGKGVI
                     HLLVDDPDKGLEVLSAAGFDVRAMRDVVVIDCQDRPGEMGEAARKVADAGINVEQAYT
                     ATQNRVVFVVDDVEKARALFA"
     gene            complement(731..2542)
                     /locus_tag="VNU01_00015"
     CDS             complement(731..2542)
                     /locus_tag="VNU01_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWH31034.1"
                     /translation="MAEELTLAAPTITELFARAIDRWSDNVAFRVKRGDEWVPITFGE
                     YGEDVRACAGALIAMGLAPGDFGSIISANRPEWHSSDLGMINAGIVSCPVYPTNSPSQ
                     VHYILEHSESKVIFCENAAQLDKVLQIRKDLPSLRAAIVFTGEHCDGDFAIAWEDFLE
                     RGREYLAVQPGEYDVRRTAATPDSLLGVIYTSGTTGPPKGTMISHHNAVWTLASLDAV
                     LEAGTDDRKLSFLPLSHVGERTSSEYLQIQQGFEIWFATSLETLGQDMVACKPTVQFV
                     VPRILEKQYDGIMKMLDGLPEENRTKALGAIELGKQRVRLEQQGKSLPEEMEAAYQQA
                     DQNLFGMARAALGYDQVKALVSGAAPISLDLLEFFNAIGVRTIEVYGQTEGHGPTTIN
                     RIDNYRLGTVGYAIPGAEVRLAEDGEILLRGGNVCLGYYKNDEATRELIDDDGWLHSG
                     DIGVMDDDGFLTITDRKKDIIITSSGKNIAPQVLEQKLKFSPWVSQAVVIGDRRPYIT
                     ALLTLDEAAAKQFAEQKSIAYADFAELTQHPDILGLIEAHVAEVNQTLSNIEQVKRFK
                     VLAEDFSVEGGTITPTLKIKRKPIAERYAAEIESLYA"
     gene            complement(2600..3730)
                     /locus_tag="VNU01_00020"
     CDS             complement(2600..3730)
                     /locus_tag="VNU01_00020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWH31035.1"
                     /translation="MTRTLWITNDFPPRAGGIEQFVHHLLERLPPDSVRVIASGWEGD
                     AAHDATLPYPVTRLRRPLLPTPGVLAEARRAIRDHQPDVVVFGAAWPLGELAGRLGVP
                     SLGFTHGHEAGLARVGFGPLIRRVARRVDALGVISGFTRRELQRWIGPYTAVHDLPPG
                     VDVRRFHPEVDRAAARRAFQVPDEAPLVVCVGRLVPRKGQDVLVEAWPRVLRRHPDAR
                     LLLVGDGPLLGRLTLRVGQLGLEHRVRLAGGVDWHKLPLCHAAADLFAMPCRSRWGGL
                     DVEGLGIVYLEAQACGVPVLAGRSGGAPEALVPGETGEVVDGTSPEAVAGAIARLLDD
                     RAALRRMGAAGRAFVERRYAWDAIGARLRELLQEIGDKRPSR"
     gene            complement(3727..5034)
                     /locus_tag="VNU01_00025"
     CDS             complement(3727..5034)
                     /locus_tag="VNU01_00025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWH31036.1"
                     /translation="MTVRAGRPLLFGLHAAAIVVLVAAVVAQVDRPLAPASPPRLDAE
                     ALFGADYLARVRAYRAPRSLVRLAVLLVGVAVPAWWGLSAPGRRLTARLAARWGAHRP
                     LRAAAVVAVTLLVAIDLVALPLVFWAGFVQEGQYGFRTQGLGGWLRDWLVVRAPVWLG
                     VAVATPVVLAVVRRLPRAWPPLLGLSAGVATAGLTLVAPLVLEPLWLTATPLPPGQVR
                     QEVELVVARAQVPVDRILVGDASRRSTKANAYVSGLGGTRRVVLFDTLLARAAPEEIA
                     VVVAHELAHDVHGDIARGVLLGAAGGVVSAYALAFALRRATAGGAVTGATDPRAVPLA
                     VALLLLLTTLSLPLQSAVSRRAEAAADWAALELTRDPAAFAGMQRALATSHLSDPDPP
                     GWEHWLWFTHPPAADRLALAAEWARRNGHAVPVSPSTTVAPRP"
     gene            complement(5031..>5598)
                     /gene="sepH"
                     /locus_tag="VNU01_00030"
     CDS             complement(5031..>5598)
                     /gene="sepH"
                     /locus_tag="VNU01_00030"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HWH31037.1"
                     /translation="AETDEAWVRRWLPPIEAERRLVLEAVEQARVTKSRLGESRDLLG
                     EAVRKNLAEKGLRADDETVEWQASKREGDSHWAVTLQYRSRGRKQRAIWHFNPDTGEL
                     DPRNALAVDVAWTRPRKAGRNRGASVAAPTRKSMQDKKASRAQKAAGKKTVGAKRAKP
                     AKKAAPAKKAAPAKKAPPAKKAASPRKR"
BASE COUNT          758 a         2139 c         1948 g          753 t
ORIGIN      
        1 ctcccacagc agcacgtccg cgccccagtc ctcgatcacg gcgggcagat cgcgcgcgaa
       61 ggcgtccgcg agcaccccgc cgaacatgtg gcgcagcgag aacagcagct tgccgcgacc
      121 gtccagctcg ctcacctcgg ggaaggtctt ggcggcctcg acctccagcc agtccaagcc
      181 gcaggccacg gcggcaaagc ccttgtcctc cacggcgccg gtgaacgacg ccgcgcaggc
      241 gaccagcacc tcgtggccgg cctcgcgcag cgcttccaga tacggcacga gcgggccgaa
      301 atggctcggc gccggctgcg tgctgacgag cacccgcatg cgacagtccc ccatcggtac
      361 ggccggtcag gcgaacagcg ccctggcctt ctcgacgtcg tcgaccacga acacgacgcg
      421 gttctgcgtg gccgtgtacg cctgctccac gttgatgccg gcgtcggcga ccttgcgcgc
      481 cgcctcgccc atctcgcccg ggcggtcctg gcagtcgatc accacgacat cgcgcatggc
      541 gcgcacgtcg aagccggccg cgctgagcac ctccaggccc ttgtcggggt cgtccaccag
      601 caggtggatg acccccttgc cctggccggt gaaggcggac acgccctcga tgttgagtcc
      661 cgcggcgccg gcggcctcgc cgatacgcgc cagcaccccg ggctggtcgt ccgggatcag
      721 tgtcaggtcc ttatgcatag agagactcga tctctgcggc gtagcgctcg gcgatcggct
      781 tgcgcttgat cttcagcgtg ggcgtgatgg tcccgccctc gacgctgaag tcctcggcaa
      841 ggaccttgaa gcgcttcacc tgctcgatgt tggacagcgt ctggttcacc tcggccacgt
      901 gcgcctcgat cagccccagg atgtccgggt gctgcgtgag ctcggcgaag tcggcgtagg
      961 cgatcgactt ctgctcggcg aactgcttgg ccgccgcctc gtccagcgtg agcagcgccg
     1021 tgatgtacgg gcgccggtcg ccgatcacca ccgcctggga cacccacggc gagaacttga
     1081 gcttctgctc gagcacctgc ggcgcgatgt tcttgccgga ggaggtgatg atgatgtcct
     1141 tcttgcggtc ggtgatggtg aggaagccgt cgtcgtccat cacgccgatg tcgccggagt
     1201 gcagccagcc gtcgtcgtcg atcagctcgc gcgtggcctc gtcattcttg tagtacccca
     1261 ggcacacgtt gccgccgcgc agcaggatct cgccgtcctc ggccaggcgc acctccgccc
     1321 cggggatcgc gtagcccacc gtgcccagac ggtagttgtc gatgcggttg atcgtggtgg
     1381 gaccgtgccc ctcggtctgg ccgtagacct cgatggtgcg cacgccgatc gcgttgaaga
     1441 actccagcag gtccagcgag atcggcgcgg cgccggacac cagcgccttg acctggtcgt
     1501 agccgagcgc ggcgcgcgcc atcccgaaca ggttctggtc ggcctgctgg taggcggcct
     1561 ccatctcctc cggcagcgac ttgccctgct gctcaaggcg cacgcgctgc ttgcccagct
     1621 cgatggcccc cagcgccttg gtgcggttct cctccggcag cccgtcgagc atcttcatga
     1681 tgccgtcgta ctgcttctcc aggatgcgcg gcaccacgaa ctgcacggtc ggcttgcacg
     1741 ccaccatgtc ctggccgagc gtctccaggc tggtcgcgaa ccagatctcg aagccctgct
     1801 ggatctgcag gtactccgag ctggtgcgct ccccgacgtg cgacagcggc aggaacgaaa
     1861 gcttgcggtc gtcggtgccg gcctccagca cggcgtccag cgacgccagg gtccacaccg
     1921 cgttgtggtg ggagatcatc gtgcccttgg gcgggccggt ggtcccggac gtgtagatca
     1981 cgccgagcag cgagtccggc gtcgccgcgg tgcggcgcac gtcgtactcg cccggctgca
     2041 ccgccaggta ctcgcggccc cgctccagga agtcctccca ggcgatggcg aaatcgccgt
     2101 cgcagtgctc gccggtgaac acgatggcgg cccgcaggct cggcaggtcc tttcggatct
     2161 gcagcacctt gtccagctgc gccgcgttct cgcagaagat caccttggac tcggagtgct
     2221 ccaggatgta gtgcacctgt gacggcgagt tggtcgggta gacggggcag ctcacgatgc
     2281 cggcgttgat catgccgagg tcggaggaat gccactccgg gcggttggcc gagatgatcg
     2341 agccgaagtc ccccggcgcc agccccatgg cgatcagcgc gcccgcgcac gcgcgcacgt
     2401 cctcgccgta ctcgccgaac gtgatcggca cccactcgtc gccgcgcttg acgcggaacg
     2461 ccacgttgtc ggaccaccgg tcgatcgcgc gcgcgaacag ctccgtgatc gtgggcgccg
     2521 ccagggtcag ctcttccgcc atgcctgtca ctcctgtcaa ggtcttcacc gccgccgttc
     2581 caccgggcgg tgatcgtagt cagcgcgacg ggcgcttgtc cccgatttct tggagcagct
     2641 cgcgcagcct cgcgccgatc gcgtcccagg cgtagcggcg ctccacgaag gcgcgtcccg
     2701 ccgcgcccat gcggcgcagg gcggcgcggt cgtccagcaa ccgggcgatc gccccggcca
     2761 cggcctcggg cgacgtgcca tcgaccacct caccggtctc gccgggcacc agcgcctccg
     2821 gcgccccgcc gctgcggccc gccagcaccg gcacgccgca ggcctgcgcc tccaggtaga
     2881 cgatgcccag gccctccacg tccaggccgc cccagcggct gcggcacggc atcgcgaaca
     2941 ggtccgccgc cgcgtgacac agcggcagct tgtgccagtc caccccgccg gccaggcgca
     3001 cccggtgctc caggccgagc tggcccacgc gcagcgtgag cctgcccagc agcggcccgt
     3061 ccccgaccag cagcagccgt gcgtccgggt ggcgccgcag cacccgcggc cacgcctcga
     3121 ccagcacgtc ctggcccttg cgcggcacca ggcgtcccac gcacaccacc agcggcgcct
     3181 cgtccggcac ctggaaggcc cggcgcgccg ccgcccgatc cacctcagga tggaagcggc
     3241 gcacatcgac gcccggcggc aggtcgtgca cggcggtgta cggcccgatc cagcgctgca
     3301 gctcgcgccg tgtgaagccg ctgatcactc ccagagcgtc gacccgccgc gccacccggc
     3361 ggatcagcgg gccgaacccg acgcgggcca ggccggcctc gtgcccgtgg gtgaagccga
     3421 gcgacggcac ccccagacgg ccggcgagtt cacccagcgg ccacgccgcc ccgaacacca
     3481 cgacatccgg ctgatggtcg cggatggccc ggcgcgcctc cgccagcacg cccggagtgg
     3541 gcagcagcgg ccggcgcagc cgcgtcacgg ggtagggcag cgtcgcgtcg tgcgcggcgt
     3601 cgccttccca cccggaggcg atcacccgca ccgagtccgg cggcaggcgc tccagcaggt
     3661 ggtgcacgaa ctgctcgatg ccgccggcgc gcggcgggaa gtcgttggtg atccagagcg
     3721 tgcgcgtcat ggcctcggcg cgacggtcgt ggaggggctg accgggacgg cgtgcccgtt
     3781 gcggcgcgcc cactcggccg ccagcgcgag ccggtcggcc gcgggaggat gggtgaacca
     3841 cagccagtgc tcccagccgg gcgggtccgg gtcggacagg tgcgaggtgg ccagggcgcg
     3901 ctgcatgccc gcgaacgcgg cgggatcccg cgtcagttcc agggcggccc agtccgcggc
     3961 cgcctcggcc cggcgcgaca ccgcgctctg cagcggcagc gacagcgtgg tcagcagcag
     4021 caacagcgcc acggcgagcg gcacggcgcg cggatcggtg gcgccggtca cggcgccgcc
     4081 cgccgtcgcc cggcgcagcg cgaaggcgag cgcgtacgcg ctcacgacgc cgccggccgc
     4141 gcccagcagc acgccgcggg cgatgtcgcc gtgcacgtca tgggccagct cgtgggccac
     4201 caccacggcg atctcctccg gggcggcccg ggccagcagc gtgtcgaaca gcaccacgcg
     4261 ccgggttccg ccaagcccgg acacgtaggc gttggccttg gtgctgcgac ggctggcgtc
     4321 acccacgagg atgcggtcga ccggcacctg cgcgcgcgcg accacgagct cgacctcctg
     4381 gcgcacctgg cccggcggca gcggcgtcgc ggtcagccac agcggctcca gcaccagcgg
     4441 cgcgaccagc gtcagcccgg cggtcgccac gccggcggag aggccgagca gcggcggcca
     4501 ggcgcgcggc agccgccgca ccacggcgag caccaccggg gtggccaccg ccacgccgag
     4561 ccagacgggt gcgcgcacga cgagccagtc ccggagccag ccgccgagcc cctgcgtgcg
     4621 gaacccgtac tggccctcct gcacgaagcc ggcccagaac accagcggca gcgccacgag
     4681 gtcgatggcc acgagcagcg tcacggccac gaccgccgcg gcgcgcagcg gccggtgcgc
     4741 cccccagcgc gccgccaggc gcgcggtcag gcgccggccg ggggcgctca ggccccacca
     4801 ggccgggacc gccacaccca cgagcagcac cgccaggcgg accagggagc gcggggcgcg
     4861 gtaggcgcgg acgcgggcga ggtagtcggc gccgaacagc gcctcggcgt cgagccgcgg
     4921 cggcgacgcc ggagccagcg gccggtcgac ctgggccacc acggccgcca ccagcaccac
     4981 gatggccgcc gcgtgcagcc cgaacaggag cgggcggccg gcccggacgg tcaccgcttg
     5041 cgcggcgaag cggccttctt cgcgggcggg gccttcttgg ccggcgcggc cttcttcgcg
     5101 ggcgcggcct tcttcgcggg cttggcgcgc ttcgcgccga cggtcttctt gcccgcggcc
     5161 ttctgtgccc tcgaggcctt cttgtcctgc atgctcttgc gcgtcggcgc ggcgacggac
     5221 gcgccgcggt tgcgcccggc cttgcgcggc cgcgtccagg cgacgtccac ggccaaggcg
     5281 ttgcgcgggt cgagctcacc cgtgtccggg ttgaagtgcc agatcgcgcg ctgcttgcgt
     5341 ccccggctgc ggtactgcag cgtcaccgcc cagtggctgt cgccctcgcg cttggaggcc
     5401 tgccactcga ccgtctcgtc gtcggcgcgc aggcccttct ccgcgaggtt cttgcgcacg
     5461 gcctcgccga gcaggtcgcg gctctcgccc aggcgcgact tcgtcacgcg cgcctgctcg
     5521 accgcctcca ggaccagccg gcgctcggcc tcgatgggcg gcagccagcg gcgcacccag
     5581 gcctcgtcgg tctccgcc
//
LOCUS       DATMKC010000002         4402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_167019, whole genome shotgun sequence.
ACCESSION   DATMKC010000002 DATMKC010000000
VERSION     DATMKC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167019"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VNU01_00035"
     CDS             complement(<1..1093)
                     /locus_tag="VNU01_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(587..589),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWH31038.1"
                     /translation="MGRGHSVQSPRSASLSSWLTDADRVVQSVCPYCAVGCGQRVFVK
                     DEQVVRIEGDDDSPISRGKLCPKGAASKSLVTSDLRLRTVRYRRPHGTEWEELDLEAA
                     MDMIADRVIESRRRHWQDADEHGVPLRRTMGFAALGGATLDNEENYLIKKLWTALGAI
                     QIENQARIUHSSTVPSLGTSFGRGGATTFQQDLANADLIVIQGSNMAECHPVGYQWVM
                     EAKRRGATIVHVDPRFTRTSATADVHVPLRAGSDIAFLGGIVNHILTNEADFRDYVVH
                     YTNAATIVSEDFRDTEDLDGLFSGWNSETGAYEDVSSWQYEGMPVASAAGRREQEATS
                     AQSTGGQGARLGGTHAPRTDPTLQDPRCVY"
     gene            complement(1230..1808)
                     /locus_tag="VNU01_00040"
     CDS             complement(1230..1808)
                     /locus_tag="VNU01_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWH31039.1"
                     /translation="MTQDPYVDPATGVLRNLLGLTTAAELATAEADITFAADVSLIDS
                     PEPGAFDLAHLCRIHRRLFGDIYGWAGEVRTVGIDKGAPFAHPQFIEEQSAMIFRHLA
                     EERYLVGLPREQFVDRLTHFLSDVNALHPFRDGNGRAQRAFFRHLAAEAGWHLDWTGV
                     TAEENVAASASSLVGDLEPLRRLLDRVVRPLE"
     gene            complement(1805..2383)
                     /locus_tag="VNU01_00045"
     CDS             complement(1805..2383)
                     /locus_tag="VNU01_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin SocA
                     domain-containing protein"
                     /protein_id="HWH31040.1"
                     /translation="MKDIATAFAAEDADLTAMKLQKLVYYVQAWHLARFGEPAFDDDI
                     QAWSQGPVVRTLYALHRGLRRVGGMAWGDARRLPESVGLIVELVRSRYGALSGDELSR
                     ITHRERPWRAARGHLPEEASSEAPIDLAELRDFHRRQGGRRSSAVLDALASVRLEGGE
                     PTPQARALAEAVVEGRLSAQEALARTVAAHRR"
     gene            complement(2487..3047)
                     /locus_tag="VNU01_00050"
     CDS             complement(2487..3047)
                     /locus_tag="VNU01_00050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH31041.1"
                     /translation="MDAVATPTVLTRASIVRPRGLGEALRLRPAGEHDVETVGAFLRG
                     LSPEARYRRFHAGVAGVSDRVVGRLLTATPPARQTLVLERGRSRGLQIVGLAQYAREN
                     DSTAEVALVVGDRWQRLGLGPRLLAALAEAAAPHYRTLSAYVQADNTPVLGLLRSATD
                     GLVTQYQGSCIRLTWAARATAAEVAA"
     gene            3229..3474
                     /locus_tag="VNU01_00055"
     CDS             3229..3474
                     /locus_tag="VNU01_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31042.1"
                     /translation="MPEGRMTWWDASAGSGRIENSGGSYLAGADDVEPDAQVKNAMVS
                     FDAERGRGMEHDRAVNVRLRRGLRNDTDQGRFGEQRS"
     gene            complement(3487..3876)
                     /locus_tag="VNU01_00060"
     CDS             complement(3487..3876)
                     /locus_tag="VNU01_00060"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWH31043.1"
                     /translation="MAEATLPGPVTAEGAEPLRISRVARLVVASLLDTLLSVLTLGIG
                     WLVWAAITAADGATPGKRLLRMQVVDVQTRRPVSWAKYVFLRGMVGQLILNVPLLGWI
                     LFFMPLWDERNQSVGAKLSNTVVVDIG"
     gene            4057..>4402
                     /locus_tag="VNU01_00065"
     CDS             4057..>4402
                     /locus_tag="VNU01_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWH31044.1"
                     /translation="MLVEIWSDVACPWCYVGKRRFERALELFEHRDAVQVRWRSFELD
                     PGAPPERAQSGPEHLAAKYGMSVAQARAANQRLRDMGAQEGLDLRLDTLRLGSTFDAH
                     RLLHLAVLGGQVQ"
BASE COUNT          652 a         1572 c         1555 g          623 t
ORIGIN      
        1 ggtacacgca gcgcgggtcc tgcagcgtcg ggtcggtgcg cggcgcgtgc gtcccgccga
       61 gccgtgcgcc ctggccgccg gtggactgcg cggaggtggc ctcctgctcg cggcgcccgg
      121 cggcgctggc cacgggcatg ccctcgtact gccacgagga gacgtcctcg tacgcgccgg
      181 tctcggagtt ccagccgctg aacagcccgt cgaggtcctc ggtgtcccgg aagtcctccg
      241 acacgatcgt cgcggcgttg gtgtagtgca cgacgtagtc ccggaagtcg gcctcgttgg
      301 tcaggatgtg gttgacgatg ccgcccagga acgcgatgtc gctgccggcg cgcagcggca
      361 cgtgcacgtc ggccgtggcg ctggtgcgcg tgaagcgcgg gtcgacgtgc acgatcgtgg
      421 cgccgcggcg cttggcctcc atcacccact ggtagccgac ggggtggcac tcggccatgt
      481 tgctgccctg gatgacgatc aggtcggcgt tggcgagatc ctgctggaac gtggtcgcgc
      541 cgccgcggcc gaacgaggtc cccagactgg ggaccgtgga gctgtgtcag atgcgcgcct
      601 ggttctcgat ctggatggcg cccagggccg tccagagctt cttgatgagg tagttctcct
      661 cgttgtccag cgtcgccccg cccagcgccg cgaagcccat cgtgcgccgc agcggcacgc
      721 cgtgctcgtc ggcgtcctgc cagtggcggc gccgggactc gatgacgcgg tcggcgatca
      781 tgtccatcgc cgcctccagg tccagctcct cccactcggt gccgtggggg cggcggtagc
      841 gcaccgtgcg caggcgcagg tcgctggtga ccaggctctt gctggcggcg cccttggggc
      901 agagcttgcc gcgcgagata gggctgtcgt cgtcgccctc gatccgcacg acctgctcgt
      961 ccttgacgaa cacgcgctgg ccgcagccga cggcgcagta cgggcacacg gactgcacca
     1021 cgcggtcggc gtcggtgagc caggacgaca gcgacgccga gcgcggcgac tgcacgctgt
     1081 ggccgcggcc catgaggtcg cccgtggcga gctggcgcag gacgggccac cgcccgaacg
     1141 cctcacccac cctgccgaag accacgcctg ccccttctct gccctgccgc ccctgcgtac
     1201 ccggtgcgag cctgccaaac accgtgcgat cactccaggg gccgcaccac acggtcgagc
     1261 agacgccgca gcggctccag gtcgccgacg aggctgctcg cgctggccgc cacattctcc
     1321 tcggccgtca ccccggtcca gtccaggtgc caccccgcct ctgccgcgag gtgccggaag
     1381 aaagcacgct gagcgcgccc gttgccgtca cgaaaaggat gcaaggcatt cacgtcgctg
     1441 aggaagtgcg tcaaccggtc cacgaattgc tctcgtggaa gcccgacgag gtagcgctcc
     1501 tctgcgaggt gccggaagat cattgccgac tgttcctcga tgaactgcgg gtgagcgaac
     1561 ggggcgccct tatcgatgcc caccgtccgg acttcccccg cccagccgta gatgtcgccg
     1621 aaaagcctcc ggtggatgcg gcacaggtgg gccaagtcaa aggcccccgg ttcgggcgag
     1681 tcgatgagag agacgtcagc cgcaaaggtg atgtcggcct ccgccgtcgc aagctcggcg
     1741 gccgtcgtga ggccgaggag gttccgcagg acgcccgtcg ccggatcgac gtacgggtcc
     1801 tgggtcatcg ccggtgagcg gcgaccgtcc gcgccagggc ctcctgcgcg ctcaggcggc
     1861 cctcgacgac agcctcagcc agagcgcgcg cttggggggt tggttccccg ccctccagac
     1921 ggacactggc gagcgcgtcg agcactgcgc tcgatcggcg ccctccctga cgacgatgaa
     1981 agtcgcggag ctcggcgagg tcgatcggcg cctcgctgct tgcctcttcg gggaggtgcc
     2041 cacgcgccgc acgccagggc ctttctcgat gcgtgatgcg ggacagctcg tcaccgctca
     2101 acgcgccgta gcgggaccgc accagttcga caatgaggcc cacgctttcg gggagacgcc
     2161 tcgcgtcgcc ccatgccatg ccgccgactc tgcgcaatcc tcggtgcaac gcgtagagcg
     2221 tgcgaaccac cgggccctgc gaccacgcct gaatgtcatc gtcgaacgcc ggctcgccga
     2281 agcgcgccaa gtgccaagcc tggacgtaat acacgagctt ctggagcttc atcgcggtca
     2341 ggtccgcgtc ctcggcggcg aacgcggtcg cgatgtcctt cacgtcagca cgctgcaccg
     2401 ctcacctcca cgccgttcac gccgtcgttc actggcgtga acacaatcct accgggaggg
     2461 tgcgacgcgc gatgggcagg cgccgctcag gccgcgacct cggccgcggt ggcgcgcgcg
     2521 gcccaggtca gccggatgca ggagccctgg tactgcgtga ccaggccgtc ggtggcggac
     2581 cgcagcaggc cgagcaccgg cgtgttgtcg gcctgcacgt aggcgctcaa tgtgcggtag
     2641 tgcggggccg ccgcctccgc cagcgccgcc agcaggcgtg ggcccaggcc cagccgctgc
     2701 cagcggtcgc cgacgaccag tgcgacctcg gcggtgctgt cgttctcgcg cgcgtactgg
     2761 gccaggccga cgatctggag gccgcgcgac cggccgcgct cgagcaccag ggtctggcgc
     2821 gccggcggcg tggccgtcag gagccggccg accacccggt cgctgacgcc ggccacgccg
     2881 gcgtggaagc gccggtagcg cgcctccggc gacaggccgc gcaggaacgc gccgaccgtc
     2941 tcgacgtcgt gctcgccggc aggccgcagg cgcagcgcct cgccgagccc gcgcgggcgg
     3001 acgatgctcg ccctggtcag caccgtcggg gtggcgacgg cgtccatggc gtcctccttc
     3061 cttcacccgg gtctcgggcg aacatcccgc aggcaagcag ggcgacgttg acgagcggtt
     3121 acgcgcacgg gacggccgcg taacgaatgg cgcgcgcgac ggttcagcgc ggcgcggccc
     3181 gggcatctgc gacggcagaa gcaccccggc cgaaggagta acgcagcgat gcctgaggga
     3241 cgtatgacct ggtgggacgc cagcgccggc agcggccgca tcgagaactc gggtggcagc
     3301 tacctcgccg gcgccgacga cgtggagccc gacgcgcagg tcaagaacgc catggtgtcc
     3361 ttcgacgccg agcgcgggcg cggcatggag cacgaccgtg cggtgaacgt gcggctgcgc
     3421 cgcgggctgc gcaacgacac cgatcagggc cgtttcggcg agcagcgctc gtaaggctcg
     3481 ccggcctcag ccgatgtcga cgacgacggt gttggagagc ttcgcgccga ccgactgatt
     3541 gcgctcgtcc cacagcggca taaagaacag tatccagccg agcagcggca cgttgaggat
     3601 gagttgaccc accatgcctc gcaggaacac gtacttggcc cagctcaccg gccgccgcgt
     3661 ctgcacgtcg accacctgca tccgcagcag gcgcttgcca ggcgtcgcac cgtccgccgc
     3721 ggtgatcgcc gcccacacca gccagccgat gccgagggtg agcaccgaca acagggtgtc
     3781 cagcagtgag gctacgacga ggcgtgcgac acgactgatc ctgagaggct ccgcaccctc
     3841 ggcggtaacc ggtcctggca aagtggcctc agccatgtgt ttcttccccc gtcatgccgc
     3901 cgcgcccgcg tgcggtcgaa ccaaggacaa cacaacacct gcggcagggc aacgatctcc
     3961 gaaccgggtg gccgggagat cggcgcccgg aggcggggaa gatcgccgcc ccgcaccgcg
     4021 ttgaccgggc gacgtcaatt gattgaggag tcaaccgtgc tggtcgagat ctggtcggac
     4081 gtcgcctgcc cgtggtgcta cgtgggcaag cgccgcttcg agcgcgcgct ggagttgttc
     4141 gagcaccgcg acgcggtgca ggtgcgctgg cgcagcttcg agctggaccc gggcgccccg
     4201 cccgagcgcg cgcagtccgg gccggagcac ctggccgcca agtacggcat gagcgtggcc
     4261 caggcgcgcg cggccaacca gcggctgcgc gacatgggcg cgcaggaggg cctggacctg
     4321 cgcctggaca ccctgcggct gggctccacc ttcgacgccc accgcctgct gcacctggcc
     4381 gtgctcggcg gccaggtgca gc
//
LOCUS       DATMKC010000003         5838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_95765, whole genome shotgun sequence.
ACCESSION   DATMKC010000003 DATMKC010000000
VERSION     DATMKC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95765"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /gene="rho"
                     /locus_tag="VNU01_00070"
     CDS             <1..1285
                     /gene="rho"
                     /locus_tag="VNU01_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWH31045.1"
                     /translation="QNSGGGDDSNRNRRSRRERRRNKGGGGGGGGGGGQQQHQRQNRP
                     QDDDPNAEPGEVRVGVLDILPEGYGFLRTTGYLPGDKDVYVSQSAVRRHGLRRGDVIE
                     GPIRQQKANEKVPALHSVQKVNGIDLDESGQVPHRPEFKDMTPLFPNERLPLETPDGP
                     ISMRIIDLMAPIGKGQRGLIVSPPKAGKTTILKELGQAIAFNNPECHLMVVLVDERPE
                     EVTDMQRSIPGEVVASTFDRPADDHTQIAELAMERAKRLVELGHDVVILLDSITRLAR
                     AYNLSAPASGRIMSGGVDSTALYPPKKFFGAARNIEHGGSLTIIASALIETGSKMDEV
                     IFEEFKGTGNMELRLDRRLEQKRIFPAIDIEASGTRKEELLLDKEELLIIWKLRRVLH
                     ALEGPGAIELLIDKMKQTRSNNQFLLQIQKTNLQG"
     gene            1358..1588
                     /gene="rpmE"
                     /locus_tag="VNU01_00075"
     CDS             1358..1588
                     /gene="rpmE"
                     /locus_tag="VNU01_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWH31046.1"
                     /translation="MKTDVHPEYVLTSVTCSCGNSFTTRGTKEQITVELCNECHPFYT
                     GKQKLVDTGGRVERYKQRYAKSAAAQAEQKNA"
     gene            1597..2670
                     /locus_tag="VNU01_00080"
     CDS             1597..2670
                     /locus_tag="VNU01_00080"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWH31047.1"
                     /translation="MSDTHDEAPAHAPHYYGGQAVIEGVMMRGAKTWAVAVRRPDGGI
                     YLERHKVSDFPQRHPLFTKPMFRGMFGLVDALTIGTRALTISANQSVEEEEQLSSKAM
                     GGSLVFALLLFVGIFIVLPNAGLAFLSRSIGDGWLYHVVEGVVRMGIFLLYLTAISLL
                     ADIRRVFQYHGAEHMTIAAWEHDEPLEPSAVRKYSTLHVRCGTNFLLMVMLLAIVVYS
                     LGGWLVPAPDGGVALKAAYHIALRVILLPVVAGLAYEGLRLGAGKDNWFVRGLMKPGL
                     WLQMITTKPPTDDQIEVAIRSFEAVVPVEHLQGRVPPELPSPLVWGEDETGPVADSPA
                     DVVGAPVADGHGAELPEEPDSGS"
     gene            2719..3807
                     /gene="prfA"
                     /locus_tag="VNU01_00085"
     CDS             2719..3807
                     /gene="prfA"
                     /locus_tag="VNU01_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWH31048.1"
                     /translation="MSDSVTRKLNEIERTHAELTEQLSDPAVLADGQRYTQLAKRHAE
                     LSEVVEVGRAHRKALEDAAAARELARDSNGEDRELFGAEAEQSEALAVDLAAQLELLL
                     LPKDPLDSKNVIVEIRAGAGGDEAGLFAGELYRMYARYAERHGWKVEELEASEQGIGG
                     VKEAVFQVIGYGAYSWLKHESGVHRVQRVPATESGGRIHTSTASVAVMPEAEEVDVHI
                     DPNDLKIDVYRSSGPGGQSVNTTDSAVRITHVPTGLVVAMQDEKSQIQNREKALRVLR
                     ARLLALEQERAAKERADLRAAQIGTGDRSEKIRTYNFPQNRVTDHRIGFTAHNLPDVM
                     QGEIDAILEALVAEEQRRRLEALAGDDA"
     gene            3870..4721
                     /gene="prmC"
                     /locus_tag="VNU01_00090"
     CDS             3870..4721
                     /gene="prmC"
                     /locus_tag="VNU01_00090"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWH31049.1"
                     /translation="MPETLGTALAALTSRLADAGVPSPAVDAELLLAHVLGTSRTALK
                     LDLNRVLDQASRWRIEALGARRARREPLQHLVGSVGFRHLDILVRPGVFIPRPETESL
                     AELAIAVTPPGGVVVEPCTGTGAVACAVAAETRAATVVATDVSADAVALARENALRCG
                     ATALQVARGDLLDPVPETLRGAVDVLVSNPPYVAAGELAYLEPEVREHDPREALVSGP
                     TGHEVSDRLIALAGQWLAPGGWLLLEVADTRAGECAARAAAAGLADVEALPDLAGRPR
                     FVRARRP"
     gene            4815..5357
                     /locus_tag="VNU01_00095"
     CDS             4815..5357
                     /locus_tag="VNU01_00095"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWH31050.1"
                     /translation="MPQPAKLTYDDLQRFPDDGLRRELIDGELFVTPAPATRHQLTVT
                     CFVGLLYNYSQERGGVVLSAPYDVKFSIHDVTEPDVLFVREPRRLGEKYLDTSPDLAV
                     EVSSPSTKGVDRIRKRALYERFGVPEYWIVDLDHDVIEAYVLRNGRYGEPTLFRRGDT
                     VTATALPGFSAAFDRLVPPA"
     gene            5367..>5838
                     /locus_tag="VNU01_00100"
     CDS             5367..>5838
                     /locus_tag="VNU01_00100"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWH31051.1"
                     /translation="MSDLPEIIPQIIPLADAERAKDAAVAALREGRLVVVPTDTAYAV
                     LADAFQPLATRKVFDAKRRTRHVPLPVVIRSPRQAVGLVEGIPEPAERLMASYWPGPV
                     TLVLPAAEGLTWDLGESRGTVMLRMPADELVTSLVQEIGPLVCTGANRAGMRSIT"
BASE COUNT          909 a         2094 c         2059 g          776 t
ORIGIN      
        1 gcagaactcc ggcggtggcg acgacagcaa ccgcaaccgc cgcagccggc gcgagcggcg
       61 ccgcaacaag ggcggtgggg gcggtggcgg cggtggcggc ggccagcagc agcaccagcg
      121 ccagaaccgc ccgcaggacg acgaccccaa cgccgagccc ggcgaggtgc gcgtcggcgt
      181 gctcgacatc ctgccggagg gctacggctt cctgcgcacg accggctacc tgccgggcga
      241 caaggacgtc tacgtcagcc agagcgcggt gcgccgccac ggcctgcgcc gcggcgacgt
      301 gatcgaaggc cccatccgcc agcagaaggc caacgagaag gtgccggccc tgcactcggt
      361 gcagaaggtc aacggcatcg acctggacga gtccggccag gtgccgcacc gtcccgagtt
      421 caaggacatg acgccgctgt tccccaacga gcggctgccg ctggagacgc ccgacgggcc
      481 catctccatg cgcatcatcg acctcatggc gcccatcggg aagggccagc gcggcctgat
      541 cgtgtcgccg ccgaaggccg gcaagaccac gatcctcaag gagctcggcc aggccatcgc
      601 cttcaacaac cccgagtgcc acctcatggt ggtgctggtg gacgagcggc ccgaggaagt
      661 gaccgacatg cagcggtcga tcccgggcga ggtggtcgcc tccacgttcg accgcccagc
      721 cgacgaccac acgcagatcg ccgagctggc gatggagcgg gccaagcgcc tggtggagct
      781 cggccacgac gtggtgatcc tgctcgactc gatcacccgc ctggcccgcg cctacaacct
      841 gtcggcgccc gcctccgggc gcatcatgtc cggcggtgtg gactcgacgg cgctgtaccc
      901 gccgaagaag ttcttcggcg cggcgcgcaa catcgagcac ggcggctcgc tcaccatcat
      961 cgccagcgcc ctgatcgaga ccggctccaa gatggacgag gtcatcttcg aggagttcaa
     1021 gggcacgggg aacatggagc tgcgcctgga ccgccggctg gagcagaagc gcatcttccc
     1081 ggccatcgac atcgaggcca gcggcacccg caaggaagag ctcctgctgg acaaggagga
     1141 gctgctgatc atctggaagc tgcggcgcgt gctgcacgcg ctggagggcc cgggcgcgat
     1201 cgagctgctg atcgacaaga tgaagcagac ccggtcgaac aaccagttcc tcctgcagat
     1261 ccagaagacg aacctgcagg gctaggcgga cccgcgtacc atcagccaga ccttgcccgc
     1321 ccttggaaac ggcgggcgcg atgagagaga gcaggcaatg aagaccgatg tgcacccgga
     1381 gtacgtgctc accagcgtga cgtgctcctg cggcaacagc ttcaccacgc gcggcaccaa
     1441 ggagcagatc acggtcgagc tctgcaacga gtgccacccc ttctacaccg gcaagcagaa
     1501 gctggtggac accggtggcc gcgtggagcg ctacaagcag cgctacgcca agagcgccgc
     1561 ggcccaggcc gagcagaaga acgcctgagc gcccgcatga gcgacacgca cgacgaggcg
     1621 ccggcgcacg cgccccacta ctacggtggc caggcggtca tcgaaggggt gatgatgcgc
     1681 ggcgccaaga cgtgggctgt cgcggtgcgc cgtcccgacg gcggcatcta cctggagcgc
     1741 cacaaggtca gcgacttccc ccagcgccac ccgctgttca ccaagcccat gttccgcggg
     1801 atgttcgggc tggtggacgc gctgaccatc ggcacgcgcg cgctcaccat ctcggccaac
     1861 cagtcggtcg aggaggaaga gcagctgtcc agcaaggcga tgggcggcag cctcgtcttc
     1921 gcgctgctgc tgttcgtcgg catcttcatc gtgctgccca acgccggcct ggcgttcctg
     1981 tcgcgctcca tcggcgacgg ctggctgtac cacgtcgtcg agggcgtggt gcgcatgggc
     2041 atcttcctgc tgtacctgac ggcgatctcg ctgctggccg acatccgccg cgtgttccag
     2101 taccacggcg ccgagcacat gacgatcgcg gcctgggagc acgacgagcc gctggagccc
     2161 agcgccgtcc gcaagtactc cacgctgcac gtgcgctgcg gcaccaactt cctgctgatg
     2221 gtcatgctgc tggccatcgt cgtgtactcc ctgggcggct ggctggtgcc cgcgcccgac
     2281 ggcggcgtgg cgctcaaggc ggcctatcac atcgccctgc gcgtgatcct gctgccggtg
     2341 gtcgccgggc tggcctacga gggcctgcgc ctgggcgccg gcaaggacaa ctggttcgtg
     2401 cgcgggctca tgaagccggg cctgtggctg cagatgatca ccaccaagcc cccgaccgac
     2461 gaccagatcg aggtggcgat ccgctccttc gaggcggtcg tgccggtgga gcacctgcag
     2521 ggccgcgtgc cgccggagct gccgtcgccg ctggtgtggg gcgaggacga gaccgggccg
     2581 gtggccgaca gtccggccga cgtcgtcggc gcgcccgtgg ccgacggcca cggcgcggaa
     2641 ctgccggagg agccggactc gggcagctag cgaccggggc ccgcacgccc ccgtcccgcc
     2701 gtccgagagg ttccaccgat gtccgacagc gtcactcgca agctcaacga gatcgagcgc
     2761 acccacgcgg agctgaccga gcagctctcc gaccccgccg tgctcgccga cggccagcgc
     2821 tacacgcagc tggccaagcg ccatgccgag ctgtccgaag tcgtggaggt cggccgcgcc
     2881 caccgcaagg cgctcgagga cgccgcggcg gcgcgcgagc tggcccgtga ctccaacggc
     2941 gaggaccgcg agctgttcgg cgccgaggcg gagcagtccg aggcgctcgc cgtggatctg
     3001 gccgcccagc tggagctgct gctgctgccc aaggacccgc tggactccaa gaacgtcatc
     3061 gtcgagatcc gggccggggc cggcggcgac gaggccggcc tgttcgccgg cgagctgtac
     3121 cggatgtacg cgcggtacgc ggagcgccac ggctggaagg tcgaggagct ggaggcctcc
     3181 gagcagggca tcggcggggt gaaggaggcc gtcttccagg tcatcggcta cggcgcctac
     3241 tcctggctca agcacgagtc cggcgtccac cgcgtgcagc gggtccccgc cacggagtcc
     3301 ggcggccgca tccacacctc cacggcctcg gtggccgtga tgccggaggc ggaggaggtc
     3361 gacgtgcaca tcgaccccaa cgatctgaag atcgacgtgt accgctcgtc cggccccggc
     3421 ggccagtccg tgaacaccac ggactccgcc gtgcgcatca cgcacgtgcc caccggcctg
     3481 gtcgtggcca tgcaggacga gaagtcgcag atccagaacc gcgagaaggc gctgcgcgtg
     3541 ctgcgggcgc gcctgctggc cctggagcag gagcgcgccg ccaaggagcg tgccgacctg
     3601 cgcgcggcgc agatcggcac gggggaccgt tccgagaaga tccgcacgta caacttcccg
     3661 cagaaccgcg tcaccgacca ccgcatcggc ttcacggcgc acaacctgcc ggacgtcatg
     3721 cagggcgaga tcgacgcgat cctggaggcg ctggtcgccg aggagcagcg ccgccggctg
     3781 gaggcgctgg ccggagacga tgcctgaccc ggggtgtgtc gactgtgtca atcgtggaat
     3841 gactttttcg acacaccccg gggtgcacgg tgcctgagac gctcgggacg gcactggccg
     3901 cgctcacgtc ccgtctcgcc gacgccggcg tgccctcgcc cgcggtcgac gccgagcttc
     3961 tcctcgccca cgtgctgggc acgtcgcgca ccgcgttgaa gctcgacctc aaccgtgtgc
     4021 tggaccaggc cagccgctgg cggatcgagg cgctgggcgc gcgccgggcg cgccgcgagc
     4081 cgctccagca cctggtgggc agcgtcggct tccgccacct cgacatcctg gtccgccccg
     4141 gcgtcttcat ccccaggccg gagacggagt cactggctga gctggcgatc gccgtgaccc
     4201 cgcctggtgg cgtggtggtc gagccgtgca ccggcaccgg cgcggtcgcc tgcgcggtgg
     4261 cggcggagac gcgggccgcg acggtggtgg ccaccgacgt gtcggcggac gccgttgcgc
     4321 tggcccgcga gaacgcgctg cgctgcggcg cgaccgcgct gcaggtggcg cggggcgacc
     4381 tgctggaccc ggtgccggag acgctgcgcg gcgcggtgga cgtgctggtc agcaacccgc
     4441 cgtacgtggc cgccggggag ctggcctacc tggagccgga ggtccgcgag cacgacccgc
     4501 gggaggcgct cgtgagcggc cccaccggcc acgaggtcag cgaccgcctg atcgccctgg
     4561 cggggcagtg gctggccccc gggggctggc tgctgctgga ggtcgccgac acgcgggccg
     4621 gggagtgcgc cgcacgcgcc gcggccgcgg gactggccga cgttgaggcc ctgccggacc
     4681 tcgcgggccg cccccgcttc gtccgcgccc gccgccccta aggttctcca cagccggagg
     4741 gcgctttctc accgggggtg ccgacgctgc gcctagggtg cgaaaatgcc cgagaccgcg
     4801 cgaaggaccc gctcatgccc cagccagcca agctcacgta cgacgacctc cagcgcttcc
     4861 ccgacgacgg cctgcgccgc gagctcatcg acggggagct gttcgtgacg cccgcgcccg
     4921 cgacgcgcca tcaactgacc gtgacctgct tcgtgggact gctctacaac tattcgcagg
     4981 aacgcggcgg cgttgtgctg agtgctccct acgacgtgaa gttcagcatc cacgacgtca
     5041 ccgagccgga cgtgctgttc gtccgcgagc cacgccggct gggggagaag tatctggaca
     5101 cgtcccccga tctggctgtg gaggtcagtt ctcccagcac caagggtgtc gaccggatcc
     5161 gcaagcgggc gctgtacgag cgtttcggcg ttcccgagta ctggatcgtc gacctcgacc
     5221 acgacgtcat cgaagcgtac gtgctgcgga acggtcgcta cggcgagccg accctgttcc
     5281 ggcggggcga caccgtcacc gcgaccgcgc tgcccggctt ctcggcggcc ttcgaccggc
     5341 tggtcccgcc cgcgtagcat cgcggcgtga gcgaccttcc cgagatcatc ccccagatca
     5401 tccccttggc ggacgccgag cgggccaagg acgccgccgt ggccgccctg cgggagggca
     5461 ggctcgtggt cgtgccgacg gacacggcct acgccgtcct ggccgacgcg ttccagccgc
     5521 tggccacccg caaggtgttc gacgccaagc ggcgcaccag gcacgtccct ctgccggtgg
     5581 tgatccgcag cccccggcag gccgtcggcc tcgtcgaggg catccccgag ccggccgagc
     5641 ggctcatggc gtcctactgg cccggccccg tcacgctggt gctgcccgcc gccgagggcc
     5701 tcacgtggga cctcggggag agccgcggca ccgtgatgct gcgcatgccg gccgacgagc
     5761 tggtcaccag cctggtgcag gagatcgggc cgctggtgtg cacgggggcc aaccgggccg
     5821 gcatgcgcag catcacgg
//
LOCUS       DATMKC010000004         9735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_22271, whole genome shotgun sequence.
ACCESSION   DATMKC010000004 DATMKC010000000
VERSION     DATMKC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22271"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VNU01_00105"
     CDS             complement(<1..381)
                     /locus_tag="VNU01_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWH31052.1"
                     /translation="MNEPFDAGRLDELQRLWTPWRMSYIRGERREVQGCPFCVLPGPE
                     FGDAEALILYRGEHAYAILNAFPYNPGHFMAVPYRHVAGYDELTDDEAAEMTALCRRG
                     VRALKATTGAQAFNVGINVGAVAGA"
     gene            complement(378..2381)
                     /gene="thrS"
                     /locus_tag="VNU01_00110"
     CDS             complement(378..2381)
                     /gene="thrS"
                     /locus_tag="VNU01_00110"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWH31053.1"
                     /translation="MITVKAPSGDSASLSEGATAQEALGALGLVKGQVIAARVDGDVR
                     DLAVTVPDGATVEAIPAASEEGRHILRHSVAHIMAQAVTDLFPGTKFAIGPPITDGFY
                     YDFDVAEPFKPEDLERIEDRMHEIIRENQAFRRRELSADEARELFADQPYKLEIIDRA
                     TGAEGLTEDETADVDPDAGNVISVYENVRPDGSVWPDLCRGPHLPTTKWVPAFKLMRV
                     AGAYWRGDERRPMLQRIYGTAWESRKELEAHLVRLEEARQRDHRKLGRDLELVHFPDE
                     LGPGLSIFLPRGAIVRKEMEDWIRAETMRRGYQPVYTPHVAREELWRISGHLENYGEL
                     MFPGMEVEAATYRLKPMNCPFHIMAFGARTRSYRELPLRISELGTVYRFERSGVVNGM
                     LRARGFTQDDSHIFCTAEQVADEVAGCLDFARDVLRTFGLGEPSRVAVSTRPEKSIGT
                     AEQWAYAEDALKRAVESSGLDYEIDAGEGAFYGPKIDVHARDAIGREWQLSTIQVDFN
                     LPERFGMVYTGSDGAEHRPFMVHRALFGSVERFFAVLLESTAGAFPTWLAPVQAVVVP
                     VSDRHVGYGREVAAALAERGLRAEVDESDDTMGAKIRKHQLLKAPYQLIVGDSEAEQR
                     TVAVRPRQGDQRKDVPLKEFAEELAAEVRTRGADAGESTGAGA"
     gene            complement(2518..3708)
                     /locus_tag="VNU01_00115"
     CDS             complement(2518..3708)
                     /locus_tag="VNU01_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWH31054.1"
                     /translation="MALSAHSHDGRLPRDQRLAVAVSVLLVLTLMAGFTSAVVKRREA
                     GVAASAAHTVVWTPGAEPLPYAAPEATEPPLPDVVVTVTSQATRSPAPAAATQPPAPS
                     SEPAPQAPAPAPQAAPQPQPAPQAAPPAPEPRMHAAGADSDSERIFREQFPVQAAARQ
                     VEGDPSTFHWAVIVGVNAYQGRTKDTIGSVADATLLRTLLLQRGWREDHILLLLDGQA
                     THDNIVRGLEWLARSTDERSSVVFSFSGHMRYQKGDPAGDGEYRDSGLWPTDNRYIWD
                     SDFVRMINAVRAQRMWLSFQGCHAGGMNDAGLEQPGRLVTYSSLERQKSYEDPEVGHS
                     VTGWFMFAEGMRDHFGDANRDGRVSVQEAHGWATPRAYTRTSQRQSMQIADGVGRPFF
                     LEIA"
     gene            3885..5096
                     /locus_tag="VNU01_00120"
     CDS             3885..5096
                     /locus_tag="VNU01_00120"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005890.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWH31055.1"
                     /translation="MAEPVIVATARSPIGRAGKGSLREMRPDDLAAVIVQAAMDKVPQ
                     LSPDQVEDLMLGCGLPGGEQGYNMGRVVTTLLGWETVPGTTITRYCASSTQTMRMAAH
                     AIKAGEGDVFIAAGVECVSRYAKGASDGLPDTMNPKFEEAAARTAKRSMGGAETWAPP
                     AGLPDIYIAMGQTAENVAQLEGVSRQEQDEFAARSQQLAEESIKNGFWEREITPVTTP
                     DGNVVTQDDGPRPGTTVEKLSQLQPVFRPDGTVTAGNACPLNDGAAAVVVMSDTKARE
                     LGITPLARIVATGVSALNPEIMGLGPVEASRQALKRAGLTIDDMDLVEINEAFAAQVL
                     PSAKQLGIPMDKLNVNGGSIAVGHPFGMTGARIMNTLLNGLQTRDGRYGLETMCIGGG
                     QGMAIIVERLS"
     gene            5102..5767
                     /gene="rpe"
                     /locus_tag="VNU01_00125"
     CDS             5102..5767
                     /gene="rpe"
                     /locus_tag="VNU01_00125"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWH31056.1"
                     /translation="MKIVPSVLPADFARLGEDCVALEKAGVDRIQWDVMDGRFVPNLT
                     FGPDVIAACRPLVSVGFEAHLMCERPEELLPRYAEAGCELIILHPETLLQPHRTYQFI
                     KQELGAKAGIALSPGTPLSLAEHCLDLIDLLLVMTVNPGFGGQAYIATMEPKIAAARA
                     MIDASGLPIELEVDGGIGPDTIAGAAAAGADVFISGSALWKYPSFSEGVADLRARAEA
                     AQT"
     gene            5881..6333
                     /locus_tag="VNU01_00130"
     CDS             5881..6333
                     /locus_tag="VNU01_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWH31057.1"
                     /translation="MGRRFFRHGELHLVLLALLERRPMHGYQLLQDLEQRFAPYWTPS
                     PGSIYPAVDALVAEGLLAPAEADGRTVFAVTGEGAAALERRREQVAEVEARTGVRLRP
                     HDAVDAAVERLLVRVRAAERQVPPQDVVIVLERAEAELEALSSERGRA"
     gene            6330..6602
                     /locus_tag="VNU01_00135"
     CDS             6330..6602
                     /locus_tag="VNU01_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HWH31058.1"
                     /translation="MSEDALDRAVARTGDERRGRRAARARACFRGHATVYLVVNLALI
                     AGWLVDRLATGEQEWWFLGTLVGWGVGLAAHYVLVRPAFRRSSRRP"
     gene            complement(6599..7390)
                     /locus_tag="VNU01_00140"
     CDS             complement(6599..7390)
                     /locus_tag="VNU01_00140"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HWH31059.1"
                     /translation="MLAFDEERRWASALGVPPSQVRRDHLLSHVLHALPHVVPEATFI
                     GGTALCRTYLPDWRVSEDIDLLVSSAGRSADQLSGGLPPLLRREFPGLHVTWRPEGAT
                     HLGHLAAGDLTIRVQLVPSDSSYRRYPTALTSVALRYGDLPAAVELSVPTRAGMAAMK
                     LNAWADRAAPRDLCDLFGLVQAGALCRDAISIAGEASAALQPAAFGDGRLPVEEEWVA
                     ALGAQMPVVPDRELAFHRVRVEVSGLCGWDRPEWRDGLRRVEGGR"
     gene            complement(7390..8040)
                     /locus_tag="VNU01_00145"
     CDS             complement(7390..8040)
                     /locus_tag="VNU01_00145"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HWH31060.1"
                     /translation="MRALPAAIARAEPALVRPWDVRDVYAKPDKELRDLARQGALLRL
                     AHGYYAVIPESERGGRWRPTIEAAGLAIAQVHYGMDNAVAMGPTAARLLGAIPRALGV
                     ATIATPKQRPVLRTEVGRVRFVTRKVDQLDVQRVRTELGPGWVTTPEQTLLDVADRPT
                     LGGMTTADAAEALAELMQRVDIGLVRDLAGRQRKRAARTRIEHAVGGQAWEQVLRV"
     gene            complement(8164..8238)
                     /locus_tag="VNU01_00150"
     tRNA            complement(8164..8238)
                     /locus_tag="VNU01_00150"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8204..8206),aa:Val,seq:cac)
     gene            complement(8300..9037)
                     /locus_tag="VNU01_00155"
     CDS             complement(8300..9037)
                     /locus_tag="VNU01_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWH31061.1"
                     /translation="MDAGRPADAHRRYEEMAVAHVMGGLDSSEGRVFRSHLLECPDCR
                     ARVGELRALAHDLADVERDERRVRAARAVETKPREGGEDVRDMDEERSSRPRVMTILG
                     VAAILALSAWNFTLRETVAQVNRDIDRSTEAAAAMEFGAAGTVEQSAAGVRGQAKTDR
                     NALVVLIDGLDDERVYGLYLLNSDGQPVFRAAVKPTEGRLFSLIDYPDGAQRVLLTLP
                     EEAPGVSPDGVTMFAATLVPPRPGAPV"
     gene            complement(9042..9365)
                     /locus_tag="VNU01_00160"
     CDS             complement(9042..9365)
                     /locus_tag="VNU01_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31062.1"
                     /translation="MTIATPTRRKFMELGSGLIAALVVGFPLGLFALVWVLGWLEAWM
                     VQPDERAAAVQALLAQEGQVDDLERAVADLMAQVADDRRRERAAPARIPARIGQSAPA
                     GSGVS"
     gene            9459..>9735
                     /locus_tag="VNU01_00165"
     CDS             9459..>9735
                     /locus_tag="VNU01_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31063.1"
                     /translation="MPQPPGQEPSGDDGFGPALRGGPGTPAPPPAPAGDDVGPVARGQ
                     AGDDFGPVLRGKAERGRARTAPGRPPRPRRAGTRRLVLAALLLPLLLA"
BASE COUNT         1374 a         3579 c         3454 g         1328 t
ORIGIN      
        1 cgcgcccgcc accgcgccga cgttgatccc gacgttgaac gcctgcgcgc ccgtcgtcgc
       61 cttcagcgcc cgcaccccgc ggcggcacag cgccgtcatc tccgccgcct cgtcgtcggt
      121 cagctcgtcg tagccggcga cgtggcggta cggcaccgcc atgaagtggc cggggttgta
      181 cgggaaggcg ttcaggatcg cgtaggcgtg ctcgccgcgg tacaggatca gcgcctcggc
      241 gtcgccgaac tccggcccgg gcagcacgca gaacgggcag ccctgcacct cccggcgctc
      301 accgcggatg taggacatgc gccacggcgt ccacaggcgc tggagctcgt ccaggcgccc
      361 ggcgtcgaac ggctcgttca cgccccggcg cccgtgctct cgcccgcgtc cgcaccccgc
      421 gtccgcacct cggctgccag ctcctcggcg aactccttca gcggcacgtc cttgcgctgg
      481 tcgccctggc gcgggcgcac cgcgacggtg cgctgctcgg cctcgctgtc gccgacgatc
      541 agctggtacg gcgccttcag cagctggtgc ttgcggatct tggcgcccat ggtgtcgtcg
      601 ctctcgtcca cctcggcccg cagcccgcgc tcggcgagcg ccgccgcgac ctcgcgcccg
      661 tagccgacgt ggcggtccga caccggcacc accacggcct gcaccggcgc gagccacgtc
      721 gggaacgcgc ccgccgtgga ctccagcagc accgcaaaga accgctccac ggagccgaac
      781 agcgcgcggt gcaccatgaa cgggcggtgc tcggcgccgt cggagccggt gtagaccatg
      841 ccgaagcgct ccggcaggtt gaagtccacc tggatcgtgg agagctgcca ctcccgcccg
      901 atcgcgtcgc gcgcgtgcac gtcgatcttc gggccgtaga acgcgccctc gcccgcgtcg
      961 atctcgtagt cgagcccgga ggactccacc gcgcgcttca gcgcgtcctc ggcgtaggcc
     1021 cactgctcgg cggtgccgat ggacttctcc gggcgggtgg acaccgcgac acgcgacggc
     1081 tcgccgaggc cgaacgtgcg cagcacgtcg cgcgcgaagt ccaggcaccc cgccacctcg
     1141 tcggcgacct gctcggcggt gcagaagatg tggctgtcgt cctgcgtgaa gccgcgcgcg
     1201 cgcagcatcc cgttgaccac gccggagcgc tcgaagcggt acacggtgcc cagctcggag
     1261 atgcgcagcg gcagctcgcg gtagctccgg gtgcgcgccc cgaacgccat gatgtggaac
     1321 gggcagttca tgggcttgag ccggtaggtc gcggcctcca cctccatgcc ggggaacatc
     1381 agctcgccgt agttctccag gtgcccggag atgcgccaca gctcctcgcg cgcgacgtgc
     1441 ggcgtgtaca cgggctggta gccgcgccgc atggtctccg cgcggatcca gtcctccatc
     1501 tccttgcgca cgatcgcgcc gcgcggcagg aagatggaaa ggcccgggcc gagctcgtcg
     1561 gggaagtgca ccagctccag gtcgcggccg agcttgcggt ggtcgcgctg gcgggcctcc
     1621 tccaggcgga ccaggtgggc ctccagctcc ttgcgggact cccacgcggt gccgtagatg
     1681 cgctgcagca tcgggcggcg ctcgtcgccg cgccagtacg cgccggcgac gcgcatcagc
     1741 ttgaacgcgg gcacccactt ggtggtgggc aggtgcgggc cgcggcacag gtccggccac
     1801 acggagccgt ccgggcgcac gttctcgtag acggagatga cgttgcccgc gtccggatcg
     1861 acatcggcgg tctcgtcctc ggtcaggccc tcggcgcccg tggcccgatc gatgatctcc
     1921 agcttgtagg gctggtcggc gaacagctcg cgcgcctcgt cggcggacag ctcacgacgg
     1981 cggaacgcct ggttctcccg gatgatctcg tgcatccggt cctcgatgcg ctccaggtcc
     2041 tccggcttga acggctcggc cacgtcgaag tcgtagtaga agccgtccgt gatcggcggc
     2101 ccgatggcga acttggtgcc ggggaacagg tccgtgacgg cctgcgccat gatgtgcgcg
     2161 acggagtggc gcaggatgtg gcggccctcc tcggaggccg ccgggatggc ctcgacagtg
     2221 gcgccgtccg gcacggtgac ggcgaggtcg cgcacgtcgc cgtccacgcg cgcggcgatc
     2281 acctggccct tgaccaggcc cagggcgccg agcgcctcct gagccgtggc gccctcggac
     2341 aggctcgcgc tgtcgccgct gggggccttg acggtgatca tcggggtgct cctggtcgcg
     2401 atcctgcgag gaagtcggaa gatccggccc acagacgggc ctcaccctcc aacttggaag
     2461 atctggccca catacgggcc tgatcttcca actcgggagg atagcggcgg ctcggagcta
     2521 ggcgatctcc aggaagaacg ggcggccgac cccgtcggcg atctgcatgc tctggcgctg
     2581 cgacgtgcgc gtgtacgcgc gcggggtggc ccagccgtgc gcctcctgca cggagaccct
     2641 gccgtcgcgg ttcgcgtcgc cgaagtggtc gcgcatgccc tcggcgaaca tgaaccagcc
     2701 ggtcaccgag tggccgacct ccgggtcctc gtaggacttc tggcgctcca gcgaggagta
     2761 ggtgaccagg cggcccggct gctccaggcc ggcgtcgttc atgccgccgg cgtggcagcc
     2821 ctggaacgac agccacatgc gctgcgcccg caccgcgttg atcatgcgca cgaagtccga
     2881 gtcccagatg tagcggttgt ccgtcggcca cagccccgag tcccggtact cgccgtcgcc
     2941 ggccgggtcg cccttctggt agcgcatgtg gccggagaag ctgaacacca cgctggagcg
     3001 ctcgtcggtg gagcgcgcca gccactccag tccgcgcacg atgttgtcgt gcgtggcctg
     3061 cccgtccagc agcagcagga tgtggtcctc gcgccagccg cgctgcagca gcagcgtgcg
     3121 cagcagcgtg gcgtcggcca cggagccgat ggtgtccttg gtgcggccct ggtaggcgtt
     3181 cacgcccacg atcaccgccc agtggaaggt ggaggggtcg ccctccacct ggcgggcggc
     3241 ggcctgcacg gggaactgct cgcggaagat gcgctcgctg tcgctgtccg cgccggcggc
     3301 gtgcatgcgc ggctcggggg ccgggggggc cgcctgcggc gcgggttgcg gctgcggcgc
     3361 cgcctggggc gcgggagcgg gtgcctgcgg cgcgggctcg gacgatggcg cgggcggctg
     3421 cgtcgcggcg gcgggcgccg gggaccgcgt ggcctgcgag gtgacggtca ccacgacgtc
     3481 gggcaggggc ggctcggtgg cttccggcgc ggcgtacggc aggggctcgg caccgggcgt
     3541 ccacaccacg gtgtgggccg cggacgcggc gacgccggcc tcccggcgct tgaccaccgc
     3601 gctcgtgaag ccggccatga gcgtgagcac gagcagcacg ctgaccgcga ccgccagtcg
     3661 ctggtcccgc ggcagcctgc cgtcgtgcga atgcgcgctc agcgccacca ctcctgtccg
     3721 ctcccgtgcg acccgaccgc acgaccgggt gtacgcaacc gatccggtag tcctaacgga
     3781 ctagtccccc gccggccacg ataacgcgca ttggtccggc gcgggcaacg gggcgataat
     3841 cgccgcctgt cactggccgt ccacctcgca cccaggagtt ccccatggct gagcccgtca
     3901 tcgtcgccac cgcccgcagc cccatcggcc gagccggaaa ggggtccctg cgcgagatgc
     3961 ggccggacga cctcgcggcg gtcatcgtcc aggccgccat ggacaaggtg ccccagctct
     4021 ccccggacca ggtcgaggac ctcatgctgg gctgcggcct gcccggcggc gagcagggct
     4081 acaacatggg gcgggtcgtc accacgctgc tgggctggga gaccgtgccc ggcaccacca
     4141 tcacccgcta ctgcgcgtcc tccacgcaga ccatgcgcat ggccgcccac gccatcaagg
     4201 ccggcgaggg cgacgtgttc atcgccgcgg gcgtggagtg cgtgagccgc tacgccaagg
     4261 gcgcctccga cgggctgccg gacaccatga acccgaagtt cgaggaggcg gcggcccgca
     4321 ccgccaagcg ctccatggga ggcgccgaga cgtgggcgcc gccggccggg ctgccggaca
     4381 tctacatcgc gatgggccag accgcggaga acgtcgcgca gctcgagggc gtgtcgcgcc
     4441 aggagcagga cgagttcgcc gcgcgctcgc agcagctcgc cgaggagtcg atcaagaacg
     4501 gcttctggga gcgtgagatc acgccggtga cgacgccgga cggcaacgtg gtgacgcagg
     4561 acgacggccc gcggccgggc accacggtgg agaagctgtc gcagctccag ccggtgttcc
     4621 gcccggacgg caccgtgacc gccggcaacg cctgcccgct caacgacggc gccgcggccg
     4681 tggtcgtgat gagcgacacc aaggcgcgcg agctgggcat cacgccgctg gcgcgcatcg
     4741 tggccacggg cgtgtccgcg ctgaacccgg agatcatggg cctggggccg gtcgaggcgt
     4801 cgcgccaggc gctcaagcgc gccgggctga ccatcgacga catggacctg gtggagatca
     4861 acgaggcctt cgccgcgcag gtgctgccgt cggccaagca gctcggcatc ccgatggaca
     4921 agctgaacgt gaacggcggc tccatcgccg tcggccaccc gttcggcatg accggcgcgc
     4981 gcatcatgaa cacgctgctc aacggcctgc agacccgcga cgggcgctac ggcctggaga
     5041 ccatgtgcat cggcggcggc cagggcatgg cgatcatcgt cgagcggctg tcctagtgtc
     5101 cgtgaagatc gtcccgtcgg tcctccccgc ggacttcgcg cgcctcgggg aggactgcgt
     5161 cgccctggag aaggcgggcg tggaccgcat ccagtgggac gtgatggacg gacgcttcgt
     5221 gcccaacctc acgttcggac cggacgtcat cgcggcgtgc cggccgctgg tgagcgtggg
     5281 cttcgaggcc cacctgatgt gcgagcggcc cgaggagctg ctgccgcgct acgccgaggc
     5341 gggctgcgag ctgatcatcc tgcacccgga gacgctgctg cagccgcacc gcacgtacca
     5401 gttcatcaag caggagctcg gcgccaaggc gggcatcgcg ctgtcgcccg gcacgccgct
     5461 gtcgctggcc gagcactgcc tggacctcat cgacctgctg ctggtgatga cggtgaaccc
     5521 aggcttcggc gggcaggcct acatcgcgac gatggagccc aagatcgccg cggcgcgggc
     5581 gatgatcgac gcgtcgggcc tgccgatcga gctggaggtc gacggcggca tcggcccgga
     5641 cacgatcgcc ggcgcggccg ccgcgggcgc cgacgtgttc atctccggct cggcgctgtg
     5701 gaagtacccc tcgttctccg agggcgtggc cgacctgcgc gcccgcgccg aggccgccca
     5761 aacctagtcc ccattcaaac gggtgcgggc accgcgggat accgcaacgg gggcacccac
     5821 ttgactcctc cgtaagcctt ccgtaatact gttacggaag gcttacggaa aggcgacggc
     5881 gtgggacggc ggttcttccg gcacggcgag ctgcacctgg tgctgctcgc gctgcttgag
     5941 cgccggccca tgcacggcta ccagctcctg caggacctgg agcagcgctt cgccccgtac
     6001 tggaccccct cccccggcag catctacccg gccgtggacg ccctggtcgc cgaggggctg
     6061 ctggcgccgg ccgaggccga cgggcgcacc gtgttcgccg tgaccggcga gggcgccgcc
     6121 gccctggagc gtcggcggga gcaggtcgcc gaggtcgagg cacgcacggg cgtgcgcctg
     6181 cgcccccacg acgccgtgga cgccgcggtg gagcggctgc tggtgcgcgt gcgggcggcc
     6241 gagcgccagg tcccgccaca ggacgtggtg atcgtgctgg aacgcgccga ggccgagctc
     6301 gaggcgctgt cgtccgaaag gggaagggca tgagcgagga cgcgctggac cgcgcggtgg
     6361 cgcgcacggg cgacgagcgg cggggacgcc gcgccgcccg tgcccgcgcc tgcttccgcg
     6421 ggcacgcgac cgtctacctg gtggtgaacc tggcgctgat cgccgggtgg ctggtggacc
     6481 ggctggcgac cggcgagcag gagtggtggt tcctcggcac gctggtcggg tggggtgtgg
     6541 ggctggcggc tcactacgtg ctggtccgcc ccgccttccg ccgctcatcc cgccggccct
     6601 agcgaccgcc ctcgacccgt cggaggccgt cgcgccactc cggccggtcc cagccgcaca
     6661 ggcccgacac ttccacgcgg acacgatgga acgcgagctc gcgatccggg accaccggca
     6721 tctgcgcccc gagggccgcc acccattcct cttcgactgg caagcggcca tcgccgaagg
     6781 cggccggctg caatgccgcc gacgcctccc ccgcgatgct gatggcgtcg cgacacaaag
     6841 cgcccgcctg gacaagcccg aacagatcgc acaggtcacg aggcgccgcg cggtcagccc
     6901 acgcattgag cttcatcgcg gccatgcccg cacgagtcgg cacggacagc tctaccgcgg
     6961 cggggagatc tccatatcgc agggctaccg aagtcagcgc cgtgggataa cggcgatagc
     7021 tgctgtcgga tggcacgagc tgcactcgga tggtgagatc gccggcagcc agatgcccca
     7081 ggtgcgtggc cccctcaggg cgccacgtga cgtggagacc gggaaactcc cgccgaagca
     7141 gcggcggcag ccctccgctg agctggtcgg cggaccggcc ggcactggac accagcaaat
     7201 cgatgtcctc ggacacgcgc cagtcgggca ggtacgtccg gcacagggcc gtcccgccga
     7261 tgaaggtcgc ctccgggacg acgtgcggca gggcatgcaa cacgtgagag agcagatgat
     7321 cgcgccgcac ctgggagggc ggtacgccca gcgcgctcgc ccaccggcgc tcctcgtcga
     7381 aggcgagcat cagactcgga gcacctgttc ccatgcctgt ccgccgacgg catgctcgat
     7441 gcgcgtccga gcggcgcgtt tgcgctggcg gccggcaagg tctcggacga gtccgatgtc
     7501 aacccgctgc atgagctcag ccagcgcctc ggcggcatcg gccgtcgtca tccctcccag
     7561 cgtcggacga tccgcgacat ccagcaacgt ctgctcaggg gtggtcaccc aacctggacc
     7621 gagctcggtc ctgaccctct gcacgtcaag ttgatcgacc ttgcgcgtga cgaagcgcac
     7681 tcgaccgacc tccgtccgca gcacaggccg ctgctttggc gttgcgattg tcgcgacgcc
     7741 aagcgcacgc ggaatcgctc ccaacaggcg ggccgctgtg gggcccatgg cgaccgcgtt
     7801 gtccatgccg tagtgcacct gagcgatggc gagcccggct gcctcgatgg tcggccgcca
     7861 ccggccaccc cgctcggact caggaatcac ggcgtagtag ccgtgcgcca gccgcaacaa
     7921 tgcgccctgg cgagcgaggt cccgcagctc cttgtcgggc ttggcgtaga cgtcgcggac
     7981 gtcccaggga cgtacgagtg ctggctcagc cctcgcaatc gctgcaggca gtgcacgcat
     8041 gagtacgact atacaacgaa aggaacctga caggttcgtt tcgttgtatg acaatcacct
     8101 caggatgctc tcctggcgtg gaaagtgcgg caaccgtcga cacggtgcac gcccggatgt
     8161 cactggtggg cccgagaggt tttgaacctc tgacctctcg cgtgtgaagc gagcgctctc
     8221 ccactgagct acgagccctt gcaagcggca aggatagccg tccgcgcaag cggcggccaa
     8281 agccggcgcc ggacgccggt cagacggggg cgccgggccg cgggggcacc agcgtggcgg
     8341 cgaacatcgt gacgccgtcg gggctcaccc ccggcgcctc ctccgggagc gtgagcagca
     8401 cccgctgcgc gccgtcggga tagtcgatga gcgagaacag ccggccctcg gtcggcttga
     8461 ccgccgcgcg gaacaccggc tgcccgtcgg agttgagcag gtacaggccg tagacgcgct
     8521 cgtcgtccag cccgtcgatc agcaccacca gcgcgttgcg gtccgtcttg gcctggccgc
     8581 gcacgcccgc ggccgactgc tccaccgtcc cggcggcgcc gaactccatg gcggcggccg
     8641 cctccgtgct gcggtcgatg tcgcggttca cctgggcgac cgtctcgcgc agggtgaagt
     8701 tccacgccga cagcgccagg atggccgcca cgcccaggat cgtcatcacg cgcgggcgcg
     8761 acgaccgctc ctcgtccatg tcgcgcacgt cctcgccccc ctcgcgcggc ttggtctcca
     8821 ccgcgcgcgc cgcccgcacc cggcgctcgt cgcgctccac gtccgccagg tcgtgcgcca
     8881 gcgcgcgcag ctcccccaca cgcgcccggc agtccgggca ctccagcagg tggctgcgaa
     8941 agacgcgccc ctcggacgaa tccagtccgc ccatgacgtg cgctacggcc atttcctcgt
     9001 agcgccggtg cgcgtcagcc ggccgtcccg cgtccatcgg ctcaggatac tccggaaccg
     9061 gccggcgccg actgcccgat tctggccgga atccgcgcgg gcgcggcccg ctcgcggcgg
     9121 cggtcgtcgg cgacctgcgc catcaggtcg gccaccgcgc gctcgaggtc gtcgacctgg
     9181 ccctcctgcg cgagcagcgc ctggaccgcg gcggcgcgct cgtccggctg gaccatccac
     9241 gcctccagcc agccgagcac ccacaccagc gcgaacagcc ccagcgggaa gcccaccacg
     9301 agcgccgcga tcagcccgct gccgagctcc atgaacttcc tcctggtggg ggtcgcgatc
     9361 gtcaccccag gtgttcccca gcgcgggccg aggaaaccgg atgcggcggc gagcgcacct
     9421 acactgcgcc ggaccctgtc gcagaaagga cctcgcgcgt gccccagccc cccggccagg
     9481 agccgtccgg cgacgacggc ttcggcccgg cgctgcgcgg cggcccgggg acgccggcgc
     9541 caccgcccgc cccggccggc gacgacgtcg gccccgtggc gcgcggccag gcgggcgacg
     9601 acttcgggcc ggtcctgcgc ggcaaggcgg agcgcggccg cgccaggacg gcgcccggca
     9661 ggccgccgcg gccccggcgc gccggcaccc gccgcctggt gctcgcggcg ctgctgctgc
     9721 cgctgctgct cgccc
//
LOCUS       DATMKC010000005         3555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_166121, whole genome shotgun sequence.
ACCESSION   DATMKC010000005 DATMKC010000000
VERSION     DATMKC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166121"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VNU01_00170"
     CDS             complement(<1..874)
                     /locus_tag="VNU01_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31064.1"
                     /translation="MVDQPFSSVPGRGIPRPGHEPVTAPGGPADHRRASEGQLLAALR
                     ARDLLALAEAWQRSAPAAHAVARRLMGPGEELERLLASVYGELWEAPPAGGPLERWAR
                     MRTFALGAAALERRGAAPAAPSVRLLLPQLPPSTRPAGDPVERVIAELPPDTAVALLR
                     AHDAGVPAAEQEHPQAPALLEAALEALARHTVGAEEAAGAGPYAEPLGDLVLGLSPNA
                     QDSTVQERMLAEPVLADRVRFLRRGRRRLEGLPPAPDLGPRVLLGVVARQQAAPAPRP
                     PREPYDPAGTEDDVV"
     gene            complement(915..2537)
                     /locus_tag="VNU01_00175"
     CDS             complement(915..2537)
                     /locus_tag="VNU01_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWH31065.1"
                     /translation="MHAVRAGEELPHDVVVVGAGVAGLVAALTLVERAPHLRVAVLDK
                     GVIGSSGSTPLAQGGMAAAVAPEDSPAWHAEDTLRAGDGLGDPRAVAVLAAEAPARVR
                     ELEAWGARFDRAPDGAYSLAWEGGQRVPRSVRTADASGAEIFRALRAAATGKVSRLQG
                     VAVELALEPAPERGVCGVWALLDQVDRSPLGPAQEGGLVLLRARGVVLATGGCGGLFA
                     STTNRDGATGDGVALAWAAGAELADMEFVQFHPTGLKPRAGDGGFWRLLLTEALRGAG
                     AHLLDAGGHRFMPARHPDAEMAPRHVVAKAILDQPAGAWLDGRHLGETAWRRDFPTVL
                     EGARAFGYDPLSEPLPVEPCQHYMIGGVVTDLWGRTTVPGLWAAGEVASTGVHGANRM
                     AGNSLSQSCVFGRRAALSLLGVLEHATAARPDPARAPVLGPAADAGAARRMPELRRVL
                     SAGAGPVRSAASLEAALKALDAADAEAAGPLPASREAVELRNGLTVGRLIARSALLRE
                     ESRGVHWREDFPGHAEAWRGVRLRLRAGAADG"
     gene            2681..3364
                     /locus_tag="VNU01_00180"
                     /pseudo
     CDS             2681..3364
                     /locus_tag="VNU01_00180"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
     gene            complement(3440..3555)
                     /locus_tag="VNU01_00185"
                     /pseudo
     CDS             complement(3440..3555)
                     /locus_tag="VNU01_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          405 a         1392 c         1372 g          386 t
ORIGIN      
        1 tcacgacgtc gtcctcggtg cccgcggggt cgtacggctc gcgtggcggg cgcggcgcgg
       61 gcgccgcctg ctgacgggcg acgacgccca gcaggacgcg cgggccgagg tccggggccg
      121 gcggcagtcc ctccaggcgg cggcgccccc ggcgcaggaa gcgcacgcgg tccgccagca
      181 ccggctcggc gagcatgcgc tcctggacgg tgctgtcctg ggcgttcggc gacaggccca
      241 gtaccaggtc gccgagcggt tcggcgtacg gccccgcgcc ggccgcctcc tccgcgccca
      301 cggtgtgccg cgccagcgct tccagcgccg cctccagcag cgcgggcgcc tgggggtgct
      361 cctgctcggc ggccggcacg cccgcgtcgt gcgcgcgcag cagtgccacg gcggtgtccg
      421 gcggcagctc ggcgatcacg cgctccaccg ggtcgccggc ggggcgcgtc gacggcggca
      481 actgcggcag caacaggcgc accgaggggg cggccggtgc cgcgccgcgc cgttccagcg
      541 ccgcggcgcc cagggcgaac gtgcgcatcc tggcccagcg ctccagcggc ccgccggccg
      601 ggggcgcctc ccacagctcg ccgtagaccg acgcgaggag gcgctccagc tcctcgccgg
      661 gccccatcag gcggcgggcg accgcgtggg cggccggcgc gctgcgctgc catgcctccg
      721 ccagggccag caggtcgcgc gcccgcagcg cggcgaggag ctggccctcg gaggcgcgac
      781 ggtggtccgc cggcccgccg ggggcggtga cgggttcgtg accgggccgc gggatcccgc
      841 ggcccggcac cgaggagaag ggctggtcca ccacgcgcaa cagggtaccc ggccgcccgg
      901 gcgtgccgga gccgtcagcc gtccgccgcg ccggcgcgca ggcgcaggcg cacgccgcgc
      961 cacgcctcgg cgtgaccggg gaagtcctcg cgccagtgca cgccccggga ctcctcgcgc
     1021 agcagggcgg accgcgcgat caggcggccg accgtcagcc cgttgcgcag ctcgaccgcc
     1081 tcgcgcgagg ccggcaacgg gccggccgcc tccgcgtccg cggcgtccag cgccttcagc
     1141 gccgcctcca ggctcgccgc cgaccggacc gggccggcgc ccgcgctgag gacgcgccgc
     1201 agctccggca tgcgccgcgc cgcgccggcg tcggcggccg gtcccagcac cggggcccgg
     1261 gccggatcgg ggcgcgccgc cgtcgcgtgc tccaggaccc ccagcagcga cagggcggcg
     1321 cggcggccga acacgcacga ctgcgacagg gagttgcccg ccatgcggtt ggccccgtgc
     1381 acgccggtcg aggcgacctc gccggccgcc cagaggcccg gcacggtggt gcggccccac
     1441 aggtccgtga ccacgccacc gatcatgtag tgctggcacg gctccaccgg cagcggctcg
     1501 gacagcgggt cgtagccgaa cgcccgggcg ccctccagga ccgtggggaa gtcgcgccgc
     1561 caggccgtct caccgaggtg gcgcccgtcc agccacgcgc cggcgggctg gtcgaggatc
     1621 gccttggcga ccacgtgccg gggcgccatc tccgcgtcgg gatggcgtgc cggcatgaac
     1681 cggtgcccgc cggcgtcgag caggtgcgcg ccggcgcctc gcagcgcctc ggtgagcagc
     1741 agccgccaga acccgccgtc ccccgcgcgc ggcttgagcc cggtcgggtg gaactggacg
     1801 aactccatgt ccgcgagctc cgccccggcg gcccacgcca gcgccacccc gtcgccggtg
     1861 gcgccgtcgc ggttggtcgt ggacgcgaac aggccgccgc agccgcccgt ggccagcacg
     1921 acgccgcggg cgcgcagcag gaccaggccg ccttcctggg ccggccccag gggggaccgg
     1981 tcgacctggt cgagcagcgc ccacacgccg cagacgccgc gctccggcgc cggctccagc
     2041 gcgagctcga ccgcgacgcc ctgcaggcgg ctgaccttgc cggttgcggc ggcgcgcaac
     2101 gcgcggaaga tctccgcgcc gctcgcgtcc gcggtgcgca cgctgcgcgg cacgcgctgc
     2161 ccgccctccc acgccagcga gtaggcgccg tccggcgcgc ggtcgaagcg ggcgccccag
     2221 gcctccagtt cccggacgcg ggcgggcgcc tcggcggcga gcaccgcgac cgcgcgcggg
     2281 tcgcccaggc cgtcgccggc gcgcagcgtg tcctcggcgt gccaggcggg gctgtcctcg
     2341 ggcgcgaccg cggcggccat gccgccctgg gccagcggtg tggagccgct ggagccgatg
     2401 acgcccttgt cgagcacggc gacgcgcagg tgcggtgcgc gctccaccag ggtcagcgcc
     2461 gcgaccagcc ccgcgacgcc cgcgccgacg accacgacgt cgtgggggag ctcttcaccg
     2521 gcgcgcaccg cgtgcatcgg cacatcctgt ccgcgcgccg gtcgcgccgc aaaggcgccc
     2581 gccgcccggt tcgtccgctc ccgcgtcgcc cggcgggctc gcgggtagcc tgccgcccgt
     2641 ggacaagaac gacggactcc ggctccttgc ggggctggcc atggtggcgc tgctgttctg
     2701 gctgttctcc gccaccggcc agcacgaggc catcacgtgg acggagttcc gcggcgcggt
     2761 cgaggagggc cgcgtggaga cggtcacgtt caccgggtcc gcgatcgagg gcgagttcgc
     2821 cgacgggacg ccgctgcgcg ccacgctacc gccggacgcg gtgatcggcc aggacggcgt
     2881 cgagggcttc ctgcgcgagc acgaggtcgc catcgacgcg cggccggagg gcaacggcgt
     2941 catcgggctg ctgctgggct ggctcgcctt cccgctgatc ttcctgggct tcctgtggta
     3001 catgagccgc caggcgcggg gccagatggg cgggatcatg caggtcggca agtcgacggc
     3061 gcgcctgtgg aagccgcacg agccgagcac ccgcttcgcc gacgtggccg gctacgagga
     3121 ggtcaagcag gaggtcgcgg aggtcgtcgc gttcctgcgc gagccggccc gcttccgcaa
     3181 ggccggcgcc gaggtgccca agggcatgct gctggtgggc ccgcccggca ccggcaagac
     3241 gctgctcgcg cgcgccgtgg ccggcgaggc cggcgtgccg ttcatctccg tgaccggctc
     3301 ggacttcatg gagatgttcg tcggcgtcgg cgccagccgc gtgcgcgatc tgttcaagac
     3361 ggcgcgcgcg aacgcgccgt gcatcgtgtt catcgacgag ctcgactcca tcggccgcaa
     3421 gcgcggcgcc ggcctgggcg gcgccgcgct tgcggccgat ggagtcgagc tcgtcgatga
     3481 acacgatgca cggcgcgttc gcgcgcgccg tcttgaacag atcgcgcacg cggctggcgc
     3541 cgacgccgac gaaca
//
LOCUS       DATMKC010000006         4239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_357210, whole genome shotgun sequence.
ACCESSION   DATMKC010000006 DATMKC010000000
VERSION     DATMKC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357210"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VNU01_00190"
     CDS             complement(<1..1449)
                     /locus_tag="VNU01_00190"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HWH31066.1"
                     /translation="MEISKVGVVGCGTMGSGIAEIVARAGIEVAFVEVSEEAVAAGRD
                     RIAGSLERAVSGGKLEAAERDAVLGRISGATEYASLAGADLVIEAVPEQLGVKQDVFR
                     ALDEVLKPEAIVATNTSSLPVMDIAVQTSRPDRVVGLHFFNPAPVMDLVELVTTVVTA
                     PEAVEAARGFAERIGKTAVVAKDRAGFIANLLLFPYLNQAVGMLEDRYATREDIDASM
                     RFGTGHPMGPLALLDLIGLDSAVNIMERMYEQFRATRYAPRPLLKHLVAAGYLGRKTG
                     RGFYTYEAPGAPTVVADEASGRAKADPATIDGWTTIGILGTGTMASGMAEVCVKAGYR
                     VVIRGRSAEKAQGAVKAIDKSLSKAVDRGKLEASAKDELLGALTATGDIADFAECDLV
                     IEAVAEDLALKREFFAELDRVTQPEALLATTTSSLPVIECAMSTSRPERVVGIHFFNP
                     AAIMRLVEIVPTVRTDPGALEQARAFTDKVGKH"
     gene            complement(1595..2017)
                     /locus_tag="VNU01_00195"
     CDS             complement(1595..2017)
                     /locus_tag="VNU01_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWH31067.1"
                     /translation="MVQFATQEWLDEYRERINSSPVYREALQSWEGTIVYLFEAEEGL
                     FPRTRCALLDVWRGECRGARLVEEADALVANAPLLIRAPYTRWKQLVRRELDPVRALM
                     QGLLRFRGDMRTIVRNVRAQSQLFTLAGEIPTEFPDDD"
     gene            complement(2121..2852)
                     /locus_tag="VNU01_00200"
     CDS             complement(2121..2852)
                     /locus_tag="VNU01_00200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWH31068.1"
                     /translation="MSEEPAAPSTPATPGAEPPPAVPWRWTDALLVLALVFLSGVVVL
                     PLVESLGAYGNVALVIVSAILTAGLTIGVARKRYGDAARLLWGHRRPTALDALVGVAA
                     AVAGYVGVTLIVVLLTAGGRAPEVQESLRQAAADPRTLPIMALGAVILVPIAEELLYR
                     GMLFQGLRDRYGRWPAIGLSGFVFGLLHVQWGDLGGTLLLLAAFYPFGMWLAWLFDRR
                     GSLVAPLVCHMLYNGGNLALFLSGR"
     gene            complement(2849..4144)
                     /gene="murA"
                     /locus_tag="VNU01_00205"
     CDS             complement(2849..4144)
                     /gene="murA"
                     /locus_tag="VNU01_00205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWH31069.1"
                     /translation="MDAFVARGGGPLHGTVRVSGAKNSALKLVAAALLAPGQAVIRNV
                     PDIADMRVMAQVLEHLGVAVRTEPGQYTLDVPDDIGDATPEHLVRMLRASIIVLGPLL
                     ARRGHARLAMPGGCNLGNRSIDMHLSGLQQMGAEIVYGADYVEAWAPRLRGADIELPF
                     ASVGATENLLMAAVTAEGTTRLSNVAREPEIADLAAYLSAMGARIEGAGSPEIVIHGV
                     PALHAADHTVVPDRIEAGTFAVAAALTGGEIRVEEAVAGHLRLPLEKLRAIGAEVDEE
                     ERAFTVRGGGRLKGADVVTLPYPGFPTDLQPQFLVLLARAHGTSIVTENVFDGRFSIL
                     DELRRLGADVSMEGHHAMIRGPRALSGTTVRATDLRAGAALVLAGLVAGGETVVTDPY
                     HVDRGYEDFAGKLRALGADVERHRPEGRPVEADLAAGVG"
BASE COUNT          583 a         1607 c         1498 g          551 t
ORIGIN      
        1 gtgcttgccg accttgtcgg tgaaggcgcg cgcctgctcc agcgcacccg ggtccgtgcg
       61 caccgtgggc acgatctcca ccagccgcat gatcgccgcc gggttgaaga agtggatgcc
      121 cacgacccgc tccgggcgcg acgtcgacat ggcgcactcg atcaccggca ggctggaggt
      181 ggtcgtggcc agcagcgcct ccggttgggt gacccggtcc agctcggcga agaactcccg
      241 cttgagcgcc aggtcctccg ccaccgcctc gatgaccagg tcgcactcgg cgaagtcggc
      301 gatgtcgccg gtcgcggtca gggcgcccag cagctcgtcc ttggcagacg cctccagctt
      361 cccgcgatcg accgccttgg acaggctctt gtcgatggcc ttcacggcgc cctgcgcctt
      421 ctccgcggac cgcccgcgga tgaccacgcg gtagccggcc ttgacgcaca cctcggccat
      481 gccggaggcc atcgtgccgg tgcccaggat gccgatggtc gtccagccgt cgatcgtggc
      541 gggatcggcc ttggcccgcc cggacgcctc gtcggcgacc accgtgggcg cacccggtgc
      601 ctcgtaggtg tagaagccgc gaccggtctt gcggcccagg tagccggcgg cgaccaggtg
      661 cttgagcagc ggccgcggcg cgtagcgcgt ggcgcggaac tgctcgtaca tgcgctccat
      721 gatgttgacg gcgctgtcca ggccgatgag gtccagcagc gccagcgggc ccatcgggtg
      781 cccggtgccg aagcgcatcg acgcgtcgat gtcctcgcgc gtggcgtagc ggtcctccag
      841 catccccacg gcctggttga ggtaggggaa cagcaggagg ttggcgatga agccggcgcg
      901 gtccttggcg accaccgcgg tcttgccgat ccgttcggcg aagccgcgcg cggcctcgac
      961 ggcctccggg gcggtcacca ccgtggtgac cagctcgacc aggtccatca ccggcgccgg
     1021 gttgaagaag tgcaggccca cgacgcggtc cgggcggctg gtctgcaccg cgatgtccat
     1081 caccggcagt gaggaggtgt tcgtcgcgac gatggcctcc ggcttgagca cctcgtccag
     1141 cgcgcggaag acgtcctgct tgacgccgag ctgctccggc acggcctcga tcaccaggtc
     1201 cgcccctgcc agcgacgcgt actccgtggc gccgctgatg cggcccagca ccgcgtcgcg
     1261 ctcggcggcc tccagcttgc ctccggacac ggcgcgctcc agcgacccgg cgatgcggtc
     1321 gcggccggcg gccacggcct cctcggagac ctcgacgaac gccacctcga tgccggcgcg
     1381 cgcgacgatc tcggcgatgc cggagcccat ggtgccgcag ccgaccacgc ccaccttgct
     1441 gatctccacg tgcctcgtcc tttccgatgc gctgcgcggg tccgttccac gccctgccgg
     1501 cgggccgcca cggcccaccg gtgagtagtc tcaccgagac ggcgcaaatt cgcagatcgg
     1561 ccgccggggc gtgacggcag cggcgcgggg cccgtcagtc gtcgtccggg aactccgtgg
     1621 ggatctcccc cgcaagcgtg aacagctggc tctgcgcccg cacgttgcgc acgatcgtgc
     1681 gcatgtcgcc cctgaagcgc agcaggccct gcatcagcgc ccgcaccggg tccagttcac
     1741 gccggacgag ctgcttccag cgcgtgtacg gcgcgcggat gagcagcggc gcgttcgcga
     1801 ccagggcgtc ggcctcctcc accagccggg cgccgcggca ctcgccgcgc cagacgtcga
     1861 ggagcgcgca gcgggtgcgc gggaacaggc cctcctccgc ctcgaacagg tagacgatcg
     1921 tgccctccca gctctggagg gcctcccggt acaccggcga ggagttgatg cgctcccggt
     1981 actcgtccag ccattcctgg gtcgcgaact gcaccatgac gattccgttt cgtgcgatgc
     2041 ggcggcgatc ctaccgggcg ccgcccaccc ccgccggggc acggcgaccc tgcccccatg
     2101 cgcctcctcg gtcgcgaacg tcagcggccg gacaggaaca gggccaggtt gccgccgttg
     2161 tagagcatgt ggcagaccag cggcgccacc aggctgccgc ggcggtcgaa cagccaggcc
     2221 agccacatgc cgaacgggta gaacgcggcc agcagcagca gcgtgccgcc gaggtcgccc
     2281 cactgcacgt gcagcagccc gaacacgaag ccggacaggc cgatggccgg ccagcgcccg
     2341 tagcggtcgc gcagcccctg gaacagcatc ccgcggtaga gcagctcctc cgcgatgggc
     2401 accaggatca ccgcgcccag cgccatgatc ggcagcgtcc tggggtcggc cgccgcctgg
     2461 cgcagggact cctgcacctc cggcgcccgg ccgcccgccg tgagcagcac gacgatcagc
     2521 gtcaccccga cgtagccggc cacggccgcg gccacgccga cgagcgcgtc cagggcggtc
     2581 ggccggcggt ggccccacag cagccgggcg gcgtcgccgt agcgcttgcg cgcgaccccg
     2641 atggtcagtc ccgccgtgag gatcgcggac acgatgacga gggcgacgtt gccgtacgcg
     2701 ccgagcgact cgaccagcgg tagcaccacg acgccgctca ggaacaccag cgccagcacc
     2761 agcagggcgt cggtccagcg ccacggcacc gccggcggtg gctcggcgcc gggcgtggcc
     2821 ggcgtggacg gcgccgcggg ctcctcgctc acccgactcc cgccgcgagg tccgcctcca
     2881 cgggccggcc ctccgggcgg tggcgctcca cgtcggcgcc cagggcccgc agcttgccgg
     2941 cgaagtcctc gtagccccgg tcgacgtgat aggggtcggt caccacggtc tcgccgccgg
     3001 ccaccaggcc ggccagcacc agcgcggcgc cggcgcgcag gtcggtggcg cgcaccgtcg
     3061 tcccgctcag ggcacggggt ccccggatca tggcgtggtg gccctccatg gacacgtcgg
     3121 cgcccagcct gcgcagctcg tccaggatgg agaagcggcc atcgaagacg ttctcggtga
     3181 cgatggacgt gccgtgcgcg cgcgccagca gcaccaggaa ctgcggctgc aggtccgtgg
     3241 ggaacccggg gtagggcagc gtcaccacgt cggcgccctt gagccgcccc ccgccccgga
     3301 cggtgaaggc acgctcctcc tcgtcgacct cggcgccgat cgcgcgcagc ttctccagcg
     3361 gcaggcgcag gtgcccggcc acggcctcct cgacgcggat ctcgccgccc gtcagcgcgg
     3421 cggccaccgc gaaggtaccg gcctcgatgc ggtccggcac gacggtgtgg tcggcggcgt
     3481 gcagcgcggg cacgccgtgg atcacgatct ccggggaacc ggcgccctcg atgcgcgcgc
     3541 ccatcgccga caggtacgcc gcgaggtcgg cgatctcggg ctctcgcgcc acattggaca
     3601 ggcgcgtggt gccctcggcg gtcaccgccg ccatgagcag gttctccgtt gcgccgacgc
     3661 tggcgaacgg cagctcgatg tcggcgcccc gcaggcgcgg cgcccaggcc tcgacgtagt
     3721 cggcgccgta gacgatctcg gcgcccatct gctgcagccc ggacaggtgc atgtcgatgg
     3781 agcggttgcc caggttgcag ccgccgggca tcgccaggcg cgcgtggccg cggcgcgcga
     3841 gcagcgggcc caggacgatg atcgacgcgc gcagcatgcg caccaggtgc tccggggtgg
     3901 cgtcgccgat gtcgtcgggc acgtcgagcg tgtactggcc gggctcggtc cgcaccgcca
     3961 cgccgaggtg ctccaggacc tgcgccatca cgcgcatgtc ggcgatgtcg ggcacattgc
     4021 ggatgaccgc ctgccccggg gccagcaggg cggcggcgac cagcttgagg gcggagttct
     4081 tggcgccgct cacacgcacc gtgccgtgca gcgggcctcc accacgagca acgaaggcat
     4141 ccatcggtga cgcgagtcta cccagccccc ggcgcggtgc ccctcggcca cccggccgcc
     4201 accaggccgc cgcccggccg gcgaatgcca ctgccgcac
//
LOCUS       DATMKC010000007         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_119151, whole genome shotgun sequence.
ACCESSION   DATMKC010000007 DATMKC010000000
VERSION     DATMKC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119151"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..453
                     /gene="hisI"
                     /locus_tag="VNU01_00210"
     CDS             97..453
                     /gene="hisI"
                     /locus_tag="VNU01_00210"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWH31070.1"
                     /translation="MSQPVDVEALRWDERGLIPAIVQQHDTGEVLMLAWMSRETLADT
                     LRRGETVFWSRSRGEAWHKGATSGNVQRVVELRADCDADVLLVRVDQLGPQGQPGVAC
                     HTGARSCFFNVIDGEG"
     gene            453..1949
                     /gene="trpE"
                     /locus_tag="VNU01_00215"
     CDS             453..1949
                     /gene="trpE"
                     /locus_tag="VNU01_00215"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWH31071.1"
                     /translation="MDGLYRPSRAEFGKLAGTHAVVPVWREVLADLSTPLAVYDRLRR
                     EPGPSFLLESVEHGERWGRYSFIGLRPLLRLVAKGSDVRVEGPAPREALDAVASGDPL
                     RVLDALLRALRAPQLPGLPPLFAGAVGYLAYDVVRFIERLPDTGADDLGMDDVRLMFP
                     GQLIAFDHLRQRLLVVTNVLVGADPGAQYDEAVAASERLVGRLSGPVEAAPVPPPVPT
                     RVTDAQANMPREDYLESVERAKEYIRAGDAFQVVPSQRLSIRTDADPFTVYRVLRVIN
                     PSPYLYLFDDGDQQIVGSSPEALVTLQGRRATIWPIAGSRPRDEDPARDLELEKQLLA
                     DEKERAEHVMLVDLARNDLGRVCELGSVAVDDFMGVVRYSHIMHIVSKVSGRVREGVG
                     AVDVARATFPAGTLTGAPKVRAMEIIDELEPTRRGLYGGGIGYFDLAGNADLCIAIRT
                     LVFRDGVAHVQAGAGVVADSVPETEYEETLNKAMALLSAVRAAEALEA"
     gene            1946..>2320
                     /locus_tag="VNU01_00220"
     CDS             1946..>2320
                     /locus_tag="VNU01_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31072.1"
                     /translation="MSARGGAAAPLLALLGGLAVAAGGAGTWVVRTSVRDVGGVPLRQ
                     ADAVPGTAFAPLALPAGLLLILVALVALALRGTLPRVAAGALLAAAFGAVAVVADGIV
                     RASVAEGRLGAAPWAAAAGAVLA"
BASE COUNT          263 a          819 c          929 g          309 t
ORIGIN      
        1 ccgccgccag caggtcggtg tcgaagcccg ccttggtgcc gtcgcggagg tcaaggacgc
       61 catggcggag gcggggctgt gcgtgaggcc ggtgcgatga gccagcccgt cgacgtcgag
      121 gcgctgcgct gggacgagcg cggcctgatc cccgcgatcg tgcagcagca cgacaccggc
      181 gaggtgctga tgctcgcgtg gatgagccgc gagacgctcg ccgacacgct gcggcgcggc
      241 gagaccgtgt tctggtcccg cagccgcggc gaggcctggc acaagggcgc caccagcggc
      301 aacgtgcagc gcgtggtgga gctgcgcgcc gactgcgacg ccgacgtgct cctggtgcgc
      361 gtggaccagc tcggcccgca ggggcagccc ggtgtcgcct gccacaccgg cgcgcgcagc
      421 tgcttcttca acgtgatcga cggggagggc tagtggacgg gctgtaccgg ccgagccgcg
      481 cggagttcgg caagctcgcg gggacgcacg ccgtggtgcc ggtgtggcgc gaggtgctgg
      541 cggacctgtc cacgccgctg gccgtctacg accgcctgcg ccgcgagccc ggcccgagct
      601 tcctgctgga gtccgtcgag cacggcgagc gctggggccg ctactccttc atcgggctgc
      661 gcccgctgct gcgcctggtc gcgaagggct ccgacgtgcg cgtcgagggc cccgcgccgc
      721 gggaggcgct ggacgccgtc gcgtccggtg accccttgcg cgtgctggac gcgctgctgc
      781 gcgcgctgcg ggcgccgcag ctgcccggcc tgccgccgct gttcgccggc gcggtcggct
      841 acctggccta tgacgtcgtg cgcttcatcg agcgcctgcc ggacaccggg gcggacgacc
      901 tgggcatgga cgacgtgcgc ctgatgttcc ccgggcagct gatcgcattc gaccatctgc
      961 gccagcgcct gctcgtggtc acgaacgtgc tggtgggcgc ggaccccggc gcgcagtacg
     1021 acgaggccgt ggcggcctcg gagcgcctgg tggggcgcct cagcgggccc gtcgaggcgg
     1081 cgcccgtgcc gccgcccgtg ccgacccgcg tcaccgacgc gcaggccaac atgccgcgcg
     1141 aggactacct ggagtcggtc gagcgcgcga aggagtacat ccgcgccggg gacgcgttcc
     1201 aggtggtgcc gtcgcagcgg ctcagcatcc gcacggacgc ggacccgttc accgtctacc
     1261 gggtgctgcg ggtgatcaac ccgtcgccgt acctgtacct gttcgacgac ggcgaccagc
     1321 agatcgtggg gtcctcaccg gaggcgctgg tcacgctgca gggccggcgg gccaccatct
     1381 ggccgatcgc cgggtcgagg ccccgcgacg aggacccggc gcgcgacctg gagctggaga
     1441 agcagctgct ggccgacgag aaggagcgcg ccgagcacgt gatgctcgtc gacctcgcgc
     1501 gcaacgacct cgggcgggtg tgcgagctgg gcagcgtggc ggtggacgac ttcatgggcg
     1561 tggtgcgcta cagccacatc atgcacatcg tctccaaggt cagcggccgt gtgcgcgagg
     1621 gggtcggcgc cgtggacgtg gcgcgcgcca ccttccccgc gggcacgctc accggcgcgc
     1681 cgaaggtgcg cgccatggag atcatcgacg agctggagcc cacgcgccgc ggcctgtacg
     1741 gcggcggcat cggctacttc gacctggcgg gcaacgccga cctgtgcatc gccatccgca
     1801 ccctggtgtt ccgcgacggc gtggcgcacg tccaggccgg cgcgggcgtg gtggccgaca
     1861 gcgtgccgga gaccgagtac gaggagaccc tcaacaaggc gatggcgctg ctgtcggccg
     1921 tgcgcgccgc ggaggcgctg gaggcatgag cgcgcgcggg ggagcggccg cgccgctgct
     1981 ggcgctgctg ggcggcctgg ccgtcgcggc cggcggggcg ggtacctggg tggtgcgcac
     2041 ctccgtccgt gacgtgggcg gcgtgccgct gcgccaggcc gacgcggtcc cgggcaccgc
     2101 gttcgcgccc ctggcgctgc cggccgggct gctgctgatc ctggtggcgc tggtggccct
     2161 ggcgctgcgc ggcacgctgc cgcgcgtcgc ggccggcgcg ctgctggccg cggcgttcgg
     2221 cgcggtggcc gtggtcgccg acggcatcgt gcgcgcgagc gtggccgagg ggcggctggg
     2281 cgcggcgccc tgggcggcgg ccgccggggc ggtcctggcg
//
LOCUS       DATMKC010000008         3755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_213754, whole genome shotgun sequence.
ACCESSION   DATMKC010000008 DATMKC010000000
VERSION     DATMKC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213754"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VNU01_00225"
     CDS             complement(<1..453)
                     /locus_tag="VNU01_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWH31073.1"
                     /translation="MIELFHDYTSPASAVAVLRAQRLADEGLPVVFEGVESVGVDAVL
                     PVTLDVLSEMESLREAAAQLGLTLRRPNGLPPTALAHALGHWAEQSGLGASWRQTCYR
                     AFWEQGADIAVPAVLRALALRAGLDGMEAEDVLGDRDRIRRVRLRTAAL"
     gene            555..1187
                     /locus_tag="VNU01_00230"
     CDS             555..1187
                     /locus_tag="VNU01_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31074.1"
                     /translation="MARTQAQVTEKVRRVWDKQAPRFDKSMAFWERRLFRGDREWACA
                     QAEGDVLEFAVGTGRNFRHYPAGVRLTGFDLSPEMVARARATASELGLDAHLTVADAT
                     ELGYADASFDTILCTYSLCSIPDAPAAVRQMHRLLRPGGRAILAEHVRSPNPVVRALE
                     ALGNLVISPFMCDDFLGEPDRYLREAGFEVGYSQRRKAGFVHRLVATKPA"
     gene            complement(1200..1664)
                     /locus_tag="VNU01_00235"
     CDS             complement(1200..1664)
                     /locus_tag="VNU01_00235"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH31075.1"
                     /translation="MSKIQKSVEVERPVSTVYNQWTQFESFPEFMEGVEQVQQMDDKR
                     LHWKAKVGPSEREWDAEIVEQEPDRVIAWRAIGEVRNDGRVQFTPMGPDKTKVDVVFE
                     MEPDGWVEKAGDALGVVDMRIQGDMERFKKFIESRGVETGAWRGEVHGGQVQ"
     gene            complement(1749..2759)
                     /locus_tag="VNU01_00240"
     CDS             complement(1749..2759)
                     /locus_tag="VNU01_00240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31076.1"
                     /translation="MNFDFSDDQYALRDAARDFFTGESGPGHVRAMWDSETGRSPELW
                     RKMAETGFVGLTVPEEHGGLGMNEIDLVLILEEAGRAALPEPLLETTAIGVPLLVEAG
                     TEAQQKEWLPKVAAGEAVVTVQLAGAPTVADAHIADLLLVQRGEELHAVPKGSFAYTA
                     QPSLDGARRVFTVDAQTSGDTRMAGGAAEAAKAFDRGAAGTAAILNGLSQRMLDMTVA
                     YVQERYQFGRPVGSFQAVKHKLAETLLVVETSKAAAWYAAYAIANDAGDRSVAASVAK
                     SYATDAEVKANYESLQCHGGIGFTWEHDLHLYLKRGKALEQAYGSATWHRGRLAERVF
                     GA"
     gene            complement(2792..3745)
                     /locus_tag="VNU01_00245"
     CDS             complement(2792..3745)
                     /locus_tag="VNU01_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31077.1"
                     /translation="MGHEIAGPTIIAFGTPEQKEKWLPPLLACDDLWCQGFSEPDAGS
                     DLANLKTSAVLEGDEYVINGQKIWTSLGRYADWMFCLVRTDKDAPKHKGITYIMVDMH
                     SPGIEVRPIMQINGDPGFAEVFFTDVRVPAWNVIGEVNDGWRVAMATLGFERGTGLGS
                     HVRFSKDLGELVSLVKAVGLDSDPLVRDRVAKLFAETEVFRHNSYRTLSRLGAGKPIG
                     PEASLNKLYWSEMETRLYEAGMQALGELAELTPQAPAVSEALGDPHATSTDGVNPVDA
                     WAHWHKQYWYSRASMIYAGTSEIQKNIISERVLGLPKEPRG"
BASE COUNT          494 a         1375 c         1307 g          579 t
ORIGIN      
        1 cagcgccgct gtgcgcagcc gcacccgccg gatgcggtcg cggtcgccca gcacgtcctc
       61 ggcctccatc ccgtccaggc ccgcccgcag cgccagcgcg cgcaggacgg cggggacggc
      121 gatgtcggcg ccctgctccc agaacgcgcg gtagcaggtc tggcgccatg aggcgccgag
      181 tccggactgc tccgcccagt ggcccagcgc gtgcgccagc gcggtcggcg gcaagccgtt
      241 cgggcgccgc agcgtcaggc cgagctgggc cgccgcctcc cgcagggact ccatctccga
      301 cagcacgtcc agcgtcaccg gcagcaccgc gtccacgccg acggactcca cgccttcgaa
      361 gacgacgggc aggccctcgt cggccagccg ctgcgcgcgc agcaccgcga ccgccgaggc
      421 cggcgaggtg tagtcgtgga agagctcgat cacccgggaa gggtagccgc cccgccgggc
      481 gccctgcggc gcggcccctt gacgggcgcg cgccggcgcg aaagactccg taatactttc
      541 gtaaggaggc gggcatggcg cggacgcagg cacaggtgac ggagaaggtc cggcgggtct
      601 gggacaagca ggcgccgcgc ttcgacaaga gcatggcgtt ctgggagcgc cgcctgttcc
      661 gcggggatcg cgagtgggcc tgcgcgcagg ccgaaggcga cgtcctggag ttcgcggtcg
      721 gcacgggccg caacttccgg cactatccgg ccggtgtgcg cctcacgggc ttcgacctca
      781 gcccggagat ggtggcccgg gcgcgcgcca ccgcgtccga gctgggcctg gacgcgcacc
      841 tgacggtggc ggacgcgacc gagctcggct acgccgacgc ctccttcgac acgatcctgt
      901 gcacctactc gctgtgctcc atcccggacg cgcccgccgc ggtgcgccag atgcaccgcc
      961 tgctgcggcc gggcggccgc gcgatcctgg ccgagcacgt gcgcagcccc aaccccgtgg
     1021 tgcgcgcgct cgaggcgctc ggcaacctcg tgatctcgcc gttcatgtgc gacgacttcc
     1081 tgggcgagcc ggaccggtac ctgcgcgagg ccggcttcga ggttggctac tcccagcgcc
     1141 gcaaggccgg cttcgtccac cgcctggtgg cgacgaagcc ggcctgagac gcacggcgcc
     1201 tactgcacct ggccgccgtg gacctcgccg cgccaggcgc cggtctccac gccgcgcgac
     1261 tcgatgaact tcttgaagcg ctccatgtcg ccctggatgc gcatgtcgac gacaccgagc
     1321 gcgtcaccgg ccttctcgac ccagccgtcg ggctccatct cgaagaccac gtcgaccttg
     1381 gtcttgtccg ggcccatggg cgtgaactgc acgcggccgt cgttgcgcac ctcgccgatg
     1441 gcgcgccagg cgatcacgcg gtccggctcc tgctcgacga tctcggcgtc ccattcgcgc
     1501 tccgacgggc cgaccttggc cttccagtgc aggcgcttgt cgtccatctg ctggacctgc
     1561 tcgacgccct ccatgaactc cgggaagctc tcgaactggg tccactggtt gtagacggtg
     1621 ctgaccggcc gttcgacttc gacgctcttc tgaatcttgg acatgcaaaa gcctcctagg
     1681 ggggtcgggg tcgtgatgac cacctacccg gtgggggcct gtcgctatgc ccgtggggcg
     1741 taaaggcgct aggcgccgaa gacccgctcg gccaaccggc cgcggtgcca cgtggccgag
     1801 ccgtaggcct gctccagcgc cttgccgcgc ttgaggtaca ggtgcaggtc gtgctcccag
     1861 gtgaagccga tgccgccgtg acactgcagc gactcgtagt tggccttgac ctcggcgtcg
     1921 gtcgcgtagg acttggcgac gctggccgcg accgagcggt ccccggcgtc gttggcgatg
     1981 gcgtaggcgg cgtaccaggc ggccgccttg gaggtctcca ccaccagcag cgtctcggcc
     2041 agcttgtgct tgaccgcctg gaagctgccc accggccggc cgaactggta gcgctcctgc
     2101 acgtaggcca cggtcatgtc gagcatgcgc tgcgacaggc cgttcaggat cgcggcggtg
     2161 ccggccgcgc cgcggtcgaa cgccttcgcg gcctcggccg cgccgccggc catgcgcgtg
     2221 tcaccggagg tctgcgcgtc cacggtgaac acgcgccgcg ccccgtccag cgacggctgc
     2281 gcggtgtagg cgaacgagcc tttgggcacc gcgtgcagct cctcgccgcg ctggacgagc
     2341 agcaggtccg cgatgtgcgc gtcggccacc gtgggggcgc cggcgagctg gacggtcacg
     2401 accgcctcgc ccgcggcgac cttcggcagc cactccttct gctgcgcctc ggtgccggcc
     2461 tcgaccagca gcggcacgcc gatcgcggtg gtctccagca gcggctccgg cagcgccgcc
     2521 cggcccgcct cctccaggat gagcaccagg tcgatctcgt tcatgcccag gccgccgtgc
     2581 tcctccggca ccgtcaggcc cacgaagccc gtctcggcca tcttgcgcca cagctccggg
     2641 ctgcggccgg tctcgctgtc ccacatcgcg cggacgtggc cggggccgga ctcgccggtg
     2701 aagaagtcgc gcgcggcgtc acgcagggcg tactggtcgt cggagaagtc gaagttcatg
     2761 tcggtgtcct ctcgcgcgtg ccggcgcggc gtcagccgcg cggctccttg ggcaggccga
     2821 gcacgcgctc ggagatgatg ttcttctgga tctcgctggt gcccgcgtag atcatcgacg
     2881 cgcgggagta ccagtactgc ttgtgccagt gcgcccaggc gtccacgggg ttgaccccgt
     2941 cggttgaggt ggcgtgcggg tcgcccaggg cctcgctgac cgcgggcgcc tgcggcgtca
     3001 gctccgccag ctcgcccagc gcctgcatgc cggcctcgta cagccgcgtc tccatctccg
     3061 accagtacag cttgttgagg ctggcctccg gaccgatcgg cttgccggcg ccgaggcgcg
     3121 acagcgtccg gtagctgttg tggcggaaca cctccgtctc ggcgaagagc ttcgcgacgc
     3181 ggtcgcgcac cagcgggtcg gagtcgaggc cgacggcttt gaccaggctc accagctccc
     3241 cgaggtcctt gctgaaccgc acgtggctgc ccaggccggt gccgcgctcg aagccgagcg
     3301 tggccatggc gacgcgccag ccgtcgttga cctcgccgat gacgttccag gcgggcacgc
     3361 gcacgtcggt gaagaacacc tcggcgaagc ccgggtcgcc gttgatctgc atgatcgggc
     3421 ggacctcgat gcccggggag tgcatgtcca ccatgatgta ggtgatgccc ttgtgcttgg
     3481 gcgcgtcctt gtcggtgcgc accaggcaga acatccagtc ggcgtaccgg cccagcgacg
     3541 tccagatctt ctggccgttg atgacgtact cgtcgccctc cagcacggcg gacgtcttga
     3601 ggttggccag gtcgctgccg gcgtccggct cgctgaagcc ctggcaccac aggtcgtcgc
     3661 aggccagcag cggcggcagc cacttctcct tctgctccgg tgtgccgaag gcgatgatcg
     3721 tcggccccgc gatctcgtgg cccagcgcct acggc
//
LOCUS       DATMKC010000009         8557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_356914, whole genome shotgun sequence.
ACCESSION   DATMKC010000009 DATMKC010000000
VERSION     DATMKC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356914"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VNU01_00250"
     CDS             <1..888
                     /locus_tag="VNU01_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWH31078.1"
                     /translation="VLRRTPQPASAAGPDLVQLLLGGEGTLGLITSVDLRIHPKPEAM
                     AFRGHRMPSFGAGLDAIRAVLRKDLRPAVVRLYDEADTNLNHPELGGGCLLVTVCEGY
                     GPLVDVEDAALRAAAAAHGGEDLGEEPARHWHAHRYDVSWRLADFIKPGGLFGDAVAV
                     DTCEVAAPWALLPDTYDAVRAALAQSMDLVMCHASHAYRDGACLYFTFGAAAQGDEAA
                     AQARYAAAWQAAMEAALRTGATITHHHGVGLLRAPYLAAELGEGGMATLRRIKAALDP
                     AGIANPGKLGLGPGAEGSP"
     gene            885..1943
                     /locus_tag="VNU01_00255"
     CDS             885..1943
                     /locus_tag="VNU01_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWH31079.1"
                     /translation="MTALPLLADVAAAAEHCSYCAKLCRYTCPVAEATGRESVTPWGI
                     DREITQAARTGTVTASTAAAVYACTGCRACGGSCLPGLDLPTHVRAARAAVVQAGLAP
                     DGVEGAAGREVAAAGALEAGATAGAELLVFPGCDSRDGDALAAVLRAAGVAWDVAREP
                     VCCGARSVDVGLADLGAEQAGRAQAALAGAARVVVMDPHCARRLAVDLADDRVVTLPR
                     LLAELVGRLALRDDVAPVVWHDPCWLARGLREHAEARDVVRAASGADAVEPEATGERT
                     RCTGGGMGFDAVFPETGRALRDARAHGLRAAGADALPVVTACPTAAQRLAAAGLDAHD
                     LAGWLATRLAHDTQEAQR"
     gene            1940..2857
                     /locus_tag="VNU01_00260"
     CDS             1940..2857
                     /locus_tag="VNU01_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWH31080.1"
                     /translation="MSSPFGPMRLIVNPRAGRHEADLKALTDGLAAQGLDFDEVRTTG
                     PGSAERLAREALEEGIRYLVAVGGDGTVHEVVNGMVADDKPVNPDAVLGVYAAGSGCD
                     FIRTFGLNRAPDVVARHFATDHVMPIDVGVAELTGPDGRPLQRYFANIAEVGYGAEAV
                     RRAAGYPRWLGKVRYLFAAWGAVRGVNAQQATIELEGKQVDRNLVNLVAANCQFFGGG
                     MKVAPRALPDDGIFNVQIFHGAKSQVFQLTSKIYKGEHIPHPGISEFQSPWVAVAPDE
                     PMLVEADGEVLGTTPVRFRLLQRAIQLKL"
     gene            2857..3441
                     /locus_tag="VNU01_00265"
     CDS             2857..3441
                     /locus_tag="VNU01_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31081.1"
                     /translation="MRALLALLLGALAAALAWTAVSAVWHLFQPAYLLSPARLLVTLA
                     ACGVALYGVRLVWQLPRPAVSLAGLVVACLWAVSFVSFIGVITLPVAALSTAVLVGVA
                     KRRGGGAGEAIATGLLVAVSLAVLLFASPWTAAVQCGDGVVRTSSRPFSGSGSRGGGM
                     SSADGTARGTFTVGGATYGYRCDGGELVEFRRLG"
     gene            complement(3438..5126)
                     /locus_tag="VNU01_00270"
     CDS             complement(3438..5126)
                     /locus_tag="VNU01_00270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWH31082.1"
                     /translation="MDEFEAVPPPPARPPAPRDEALERLGAQPWDVLVVGGGITGAGI
                     ALDAASRGLRVALVERDDLASGTSSRSSSLVHGGLRYIEQYGFGLVRESAAERERLRK
                     LAPHLVRPLRFTIPHQGRKRTSLLGVGLWMYDSIALFRGPSRHRRLEPHDVVERLPGL
                     TAQLDGFGYEYSDCRTDDVRLVLAVVRAAERHGATVLTRAEVVEVRRAAGRVVGATVR
                     DRLSGRSVGVSARWTVSATGVWADDLRAMAEAGTPMLRPSKGVHLVFPAELLRVRTAG
                     VVPSVAGDRRSVFLIPWGGQVLVGTTDDSYDGPLDRPAVDQRDADYLCAAVNRAFGTA
                     LSAADAVGAYAGLRPLLHGEQDKEVGTDRLSRRHALLVETPGLLTVTGGKLTTYRAMA
                     VEAVDRLADDLGGAKARRRSGTGRIALGLRGDFGEAVARTELLCERVGVDPALARGLV
                     ERHGDDAPELVELAARHGEDGPLAPGAPYLAVEARWAVEREHALAVADVLQRRLKVAR
                     RVADGGASAAPRVAALLASAQGWGSAQAERSVAEHLAGVRDERGPVPLAAPAAV"
     gene            5196..6293
                     /locus_tag="VNU01_00275"
     CDS             5196..6293
                     /locus_tag="VNU01_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWH31083.1"
                     /translation="MPTPYRDRLARAERAMAAARIDALLVGAGADLRYLTGYVAPPLE
                     RLTLLVARADGAHTLIVPRLERAAAEAHADGVALASFGETDSPYALASQVLGGLEGGS
                     FGVGDQLWSMFLLGLQDALPKAAWRRATPVLRELRMRKDAAELDALRRAAQAIDRVHR
                     RVPALLAPGRTETEVARDIAAAMVEEGHERADFTIVGSGPNGASPHHEAGERRLEAGD
                     AVVVDIGGPMPDGYFSDCTRNYVVGQAPDGYREAHAALEAAQRAACEAVRPGASAASV
                     DAAAREVLREAGYAEWFIHRTGHGIGLDVHEEPYIVEGNDLVLEAGMTFSVEPGIYLP
                     DRFGLRIEDIVAVTGDGVERLNTLEREAVVA"
     gene            6290..6781
                     /locus_tag="VNU01_00280"
     CDS             6290..6781
                     /locus_tag="VNU01_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH31084.1"
                     /translation="MSDPTDGQIRVDRARLEEVQPLAAEYRRDASRQGSTVEPPLPTG
                     AVFWVARDAAREILGYAAGQLRPEGLVLGPFYVRERARRLGVGLKLLRAIEEWATGAR
                     IPVVEVSVAADNPGGVRFLEAAGYRTRRLLMAREDAGTGLPEDDEAAADAATGDGVTR
                     TTP"
     gene            6778..7923
                     /locus_tag="VNU01_00285"
     CDS             6778..7923
                     /locus_tag="VNU01_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31085.1"
                     /translation="MTVARLEPEQRDLLELVRAFARNEVQPRADAYERDERFPADLFA
                     QLGKMDLAGLPFAVEDGGGGQPFTVYLRVVEELSRAFMALGLGLSVHTLSTWAVSAYA
                     PEPLRAQVLPRMTAGDWLGAYSLSEPGSGSDAAGLSTRARLDGEHWVVDGVKAWVTHQ
                     GIAQVYVLMCRTGEHKTRGVSALLVPGDTPGLSFPAVERKMGMRASPTGQLVFDGARV
                     PAANLLGEEGEGFKIAMAALDGGRLGIAAASVGLAQRCLDESVAYARQRTQFGRPIAD
                     FQGVAFMLADMATAVEAARALYLEAADRRDLGLAYARLAAMAKLSASDTAMKAAVDAV
                     QLHGGYGYTEDYPVERLMREAKMLQIVEGTNQVQRMVISRDLLRDGA"
     gene            7927..8253
                     /locus_tag="VNU01_00290"
     CDS             7927..8253
                     /locus_tag="VNU01_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31086.1"
                     /translation="MPATPHGPRDRTTVRRALGAGAAVWAACLALWTLLGVAGTPGLE
                     HVPLYAALGAPFAGIAAALWLWLAILLDVLAGERPSRRRWLWAAGLTVVTAFVLPVML
                     SVAFAI"
     gene            8281..>8557
                     /locus_tag="VNU01_00295"
     CDS             8281..>8557
                     /locus_tag="VNU01_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31087.1"
                     /translation="MPEFTCAECGSAFSLPDAVLAKYPGWTPRQCRSCRDAGGGAAAK
                     APTAKAPTAKAPAAARGGRKRRAVEENLPVAEVLARHAGGPQDGVFTD"
BASE COUNT          978 a         3208 c         3350 g         1021 t
ORIGIN      
        1 gtcctgcggc gcacgccgca gcccgcctcg gcggccggcc cagacctggt ccagctcctg
       61 ctcggcgggg agggcacgct ggggctgatc acgtcggtgg acctgcgcat ccaccccaag
      121 cccgaggcca tggcgttccg cgggcaccgc atgccgtcct tcggcgcggg cctggacgcg
      181 atccgcgcgg tgctgcgcaa ggacctgcgc ccggccgtgg tgcgcctgta cgacgaggcg
      241 gacacgaacc tcaaccaccc ggagctgggc ggcgggtgcc tgctggtcac cgtctgcgag
      301 ggctacgggc cgctggtcga cgtggaggac gcggcgttgc gcgccgccgc cgccgcccac
      361 ggcggcgagg acctggggga ggagccggcc cgccactggc acgcccaccg ctacgacgtg
      421 agctggcgcc tggcggactt catcaagccg ggcgggctgt tcggcgacgc ggtcgccgtg
      481 gacacctgcg aggtcgcggc gccgtgggcg ctgctgccgg acacctacga cgccgtgcgc
      541 gcggcgctgg cacagtcgat ggacctggtg atgtgccacg ccagccacgc ctaccgcgac
      601 ggcgcgtgcc tgtacttcac gttcggcgcc gccgcgcagg gcgacgaggc ggccgcccag
      661 gcgcgctacg cggccgcctg gcaggccgcc atggaagcgg cgctgcgcac cggcgcgacc
      721 atcacccacc accacggcgt cggcctgctg cgcgcgccgt acctggccgc ggagctgggc
      781 gagggcggca tggcgacgct gcggcgcatc aaggcggccc tggacccggc cggcatcgcc
      841 aaccccggca agctcggcct cggccccggc gcggaggggt cgccgtgacc gcgctgccgc
      901 tgctcgccga cgtcgccgcg gccgccgagc actgctccta ctgcgccaag ctgtgccgct
      961 acacctgccc ggtggcggag gcgaccgggc gggagtcggt gacgccgtgg ggcatcgacc
     1021 gggagatcac ccaggcggcc cggaccggca cggtcaccgc gtccacggcc gccgccgtgt
     1081 acgcgtgcac cgggtgccgg gcctgcggcg gctcctgcct gccgggcctg gacctgccga
     1141 cgcacgtgcg cgccgcccgg gcggccgtgg tgcaggccgg gctggcgccc gacggcgtcg
     1201 agggggccgc cgggcgggag gtcgccgccg ccggcgcgct ggaagcgggc gccaccgccg
     1261 gcgcggaact gctggtcttc ccgggctgcg actcccgcga cggcgacgcg ctggccgccg
     1321 tgctgcgcgc cgccggggtg gcgtgggacg tcgcgcgcga gccggtgtgc tgcggcgcgc
     1381 gcagcgtgga cgtcggcctg gccgacctgg gggcggagca ggccggtcgc gcgcaggcgg
     1441 cgctggccgg cgcggcgcgg gtggtggtca tggacccgca ctgcgcgcgc cgcctggccg
     1501 tggacctggc cgacgaccgg gtcgtcacgc tgccgcgcct gctcgccgaa ctggtcggcc
     1561 ggctggcgct gcgcgacgac gtcgcgccgg tggtctggca cgacccctgc tggctggcgc
     1621 gcgggctgcg cgagcacgcc gaggcgcgcg acgtcgtgcg cgccgccagc ggcgccgacg
     1681 cggtggagcc ggaagccacc ggggagcgca cgcgctgcac cggcggcggc atgggcttcg
     1741 acgcggtgtt cccggagacg gggcgggcgc tgcgcgacgc gcgcgcgcac gggctgcgcg
     1801 cggccggcgc ggacgccctg cccgtggtga ccgcctgccc cacggccgcg cagcgcctgg
     1861 ccgcggcggg cctggacgcc cacgacctcg ccggctggct ggccacccgg ctcgcgcacg
     1921 acacgcagga ggcacagcga tgagcagtcc cttcggcccc atgcggctga tcgtgaaccc
     1981 gcgcgccggg cgccacgagg cggacctgaa ggcgctcacg gacggcctgg ccgcccaggg
     2041 cctggacttc gacgaggtgc gcacgaccgg gcccggttcg gccgagcggc tcgcgcgcga
     2101 ggcgctggag gagggcatcc gctacctcgt cgcggtgggc ggcgacggca ccgtgcacga
     2161 ggtcgtgaac gggatggtcg ccgacgacaa gccggtgaac cccgacgccg tcctgggcgt
     2221 gtacgcggcc ggctccggct gcgacttcat ccgcacgttc ggactgaacc gcgcgcccga
     2281 cgtggtggcc cgccacttcg ccacggacca cgtcatgccg atcgacgtgg gcgtggccga
     2341 gctcaccggc ccggacggcc gcccgctgca gcgctacttc gccaacatcg ccgaggtcgg
     2401 ctacggcgcc gaggcggtgc ggcgcgcggc cggctacccg cgctggctgg gcaaggtgcg
     2461 ctacctgttc gccgcctggg gcgccgtgcg cggcgtcaac gcccagcagg ccaccatcga
     2521 gctggagggc aagcaggtcg accgcaacct ggtgaacctg gtggccgcca actgccagtt
     2581 cttcggcggc ggcatgaagg tcgcgccgcg cgcgctgccc gacgacggca tcttcaacgt
     2641 gcagatcttc cacggcgcca agagccaggt gttccagctc acgtccaaga tctacaaggg
     2701 cgagcacatc ccgcatccgg ggatctccga gttccaatcc ccgtgggtgg cggtggcgcc
     2761 ggacgagccg atgctggtgg aggccgacgg cgaggtcctg ggcacgaccc cggtgcgctt
     2821 ccgcttgctc cagcgggcga tccaactcaa gctgtgatgc gcgcgctgct ggcgctgctg
     2881 ctcggggcgc tggccgccgc gctggcctgg accgccgtca gcgccgtctg gcacctgttc
     2941 cagcccgcct acctgctgtc gccggcgcgc ctgctggtga cgctggcggc gtgcggcgtg
     3001 gcgctgtacg gggtgcgcct ggtgtggcaa ctgccgcggc cggccgtgtc cctcgcgggc
     3061 ctcgtggtgg cgtgcctttg ggcggtgagc ttcgtgagct tcatcggggt catcacgctg
     3121 ccggtcgcgg cgctgagcac cgccgtcctg gtgggcgtgg ccaagcggcg cggcggcggc
     3181 gccggcgagg ccatcgcgac cggcctcctc gttgccgtca gcctggccgt gctgctgttc
     3241 gcctcgccgt ggaccgccgc cgtgcagtgc ggcgacggcg tggtgcgcac gtcgagccgt
     3301 ccgttcagcg ggtccggctc ccgcggcggc ggcatgtcat ccgccgacgg gacggcacgc
     3361 ggcacgttca ccgtcggggg cgcgacctac ggctatcgct gcgacggcgg cgagctggtg
     3421 gagttccgcc gcctcggtta gacggcggcg ggtgccgcca gcgggacggg gccgcgctcg
     3481 tcacggacgc ccgccaggtg ctcggcgacg ctgcgctccg cctgcgcgct gccccagccc
     3541 tgcgcggagg ccagcagcgc ggcgacccgc ggcgccgcgg aggcgccgcc gtcggcgacg
     3601 cggcgcgcca ccttgagccg gcgctggagc acgtcggcca cggccagcgc gtgctcccgc
     3661 tccaccgccc agcgcgcctc gacggccagg tagggcgcgc ccggggccag cgggccgtcc
     3721 tcgccgtgcc gcgccgccag ctccaccagc tccggcgcgt cgtcgccgtg gcgctccacc
     3781 agcccgcgcg ccagcgcagg gtccacaccc acccgctcgc acagcagctc ggtgcgggcc
     3841 accgcctcgc cgaagtcgcc ccgcaggccc agggcgatgc ggccggtgcc gctgcggcgg
     3901 cgcgccttcg cgccgcccag gtcgtcggcc aggcggtcca ccgcctccac cgccatggcg
     3961 cggtaggtgg tcagcttgcc gccggtgacg gtcagcaggc ccggggtctc gaccaggagc
     4021 gcgtggcggc gcgacagccg gtccgtgccg acctccttgt cctgctcgcc gtgcagcagc
     4081 ggccgcaggc ccgcgtacgc gccgaccgcg tcggcggcgg acagggccgt gccgaacgcg
     4141 cggttcacgg cggcgcacag gtagtcggcg tcgcgctggt ccacggccgg gcggtccagc
     4201 ggcccgtcgt aggagtcgtc cgtggtgccg accaggacct ggccgcccca cgggatcagg
     4261 aacaccgagc ggcggtcgcc ggccacggac ggcaccacgc ccgccgtgcg cacccgcagc
     4321 agctccgccg ggaacaccag atgcacgccc ttggaggggc gcagcatggg cgtgccggcc
     4381 tccgccatgg cgcgcaggtc gtcggcccac acgccggtcg ccgacaccgt ccagcgcgcc
     4441 gacacgccga cgctgcgccc gctgaggcgg tcgcgcaccg tcgcgcccac cacgcggccg
     4501 gcggcgcggc gcacctccac cacctccgca cgggtcagca ccgtggcgcc gtggcgctcg
     4561 gcggcgcgca ccacggccag gaccaggcgc acgtcgtcgg tgcggcagtc gctgtactcg
     4621 tagccgaagc cgtcgagctg cgcggtcagt cccggcaggc gctccaccac gtcgtggggc
     4681 tccaggcggc ggtggcggga cggcccccgg aacagcgcga tcgagtcgta catccacagg
     4741 ccgacgccca gcaggctggt gcgcttgcgg ccctggtggg ggatcgtgaa gcgcagcggg
     4801 cgcaccaggt gcggggccag cttgcgcagc cgttcgcgct cggccgcgct ctcgcggacc
     4861 aggccgaagc cgtactgctc gatgtagcgc aggccgccgt gcaccaggct ggaggagcgc
     4921 gacgaggtgc cggaggccag gtcgtcgcgc tccaccaggg ccacccgcag gccgcgggac
     4981 gcggcgtcca gcgcgatgcc tgcgccggtg atgccgccgc cgaccaccag gacgtcccac
     5041 ggctgcgcgc ccagccgttc cagcgcctcg tcccgagggg cgggagggcg cgcaggcgga
     5101 ggcggcacgg cctcgaactc gtccatgtgc gcctcagtgt aggcagcggc ggccgctcta
     5161 tgatcgccgc gccgccgaac aggagcccgc ccccgatgcc cacgccgtac cgcgaccgcc
     5221 tggcccgcgc cgagcgggcc atggccgccg cccggatcga cgccctgctg gtcggcgcgg
     5281 gggccgacct gcgctacctg accggctacg tcgcgccgcc gctggagcgc ctgacgctgc
     5341 tggtcgcgcg cgccgacggg gcccacacgc tgatcgtgcc gcgcctggag cgggccgccg
     5401 cggaggccca cgccgacggc gtggcgctgg cgtccttcgg cgagaccgac tcgccgtacg
     5461 cgctggcgtc ccaggtgctc ggcgggctgg agggcggcag cttcggcgtc ggcgaccagc
     5521 tctggtcgat gttcctgctg ggcctgcagg acgccctgcc caaggccgcc tggcgacgcg
     5581 cgacgccggt gctgcgggag ctgcgcatgc gcaaggacgc cgccgagctg gacgcgctgc
     5641 gccgcgcggc gcaggcgatc gaccgggtgc accggcgcgt gcccgcgctg ctggcacccg
     5701 ggcgcacgga gaccgaggtg gcgcgcgaca tcgcggcggc catggtcgag gagggccacg
     5761 agcgcgccga cttcaccatc gtgggctccg ggcccaacgg cgcgtcgccg caccacgagg
     5821 ccggcgagcg gcgcctggag gccggcgacg ccgtggtggt cgacatcggc gggcccatgc
     5881 cggacggcta cttctccgac tgcacgcgca actacgtggt cgggcaggcg ccggacggct
     5941 accgggaggc ccacgcggcg ctggaggccg cgcagcgggc ggcgtgcgag gcggtgcggc
     6001 cgggggccag cgcggcgtcg gtggacgccg ccgcgcgcga ggtgctgcgc gaggccggct
     6061 acgccgagtg gttcatccac cgcaccgggc acggcatcgg cctggacgtg cacgaggagc
     6121 cctacatcgt ggagggcaac gacctggtgc tggaggccgg catgacgttc tcggtcgagc
     6181 cgggcatcta cctgccggac cggttcggcc tgcgcatcga ggacatcgtc gccgtcaccg
     6241 gcgacggcgt ggagcggctc aacaccctgg agcgagaggc ggtggtcgcg tgagcgatcc
     6301 gacggacggg cagatccgcg tcgaccgggc gcgcctggag gaggtgcagc cgctggcggc
     6361 ggagtaccgg cgggacgcct cccgccaggg cagcacggtg gagccgccgc tgcccaccgg
     6421 cgcggtgttc tgggtggcgc gcgacgccgc ccgggagatc ctcggctacg ccgccgggca
     6481 gttgcgcccg gaaggcctgg tgctggggcc gttctacgtg cgcgagcggg cccggcgtct
     6541 gggcgtgggc ctgaagctgc tgcgcgccat cgaggagtgg gcgacgggcg cgcgtatccc
     6601 cgtggtggag gtctccgtcg ccgcggacaa ccccggcggc gtgcgcttcc tggaggcggc
     6661 cggctaccgc acccggcgcc tgctgatggc gcgcgaggac gccgggacgg gccttccgga
     6721 ggacgacgag gcggcggcgg acgccgccac cggcgacggc gtcacccgga ccacgccatg
     6781 acggtcgcac gcctggagcc ggagcagcgc gacctgctgg agctggtgcg cgcgttcgcg
     6841 cgcaacgagg tgcagcccag ggcggacgcc tacgagcgcg acgagcgctt ccccgccgac
     6901 ctgttcgccc agctcggcaa gatggacctg gccggcctgc ccttcgccgt ggaggacggc
     6961 ggcggcggcc agccgttcac ggtgtacctg cgcgtggtgg aggagctgtc ccgcgcgttc
     7021 atggcgctgg gcctgggcct gtcggtgcac acgctgtcga cgtgggcggt gtcggcctac
     7081 gcgccggagc cgctgcgcgc ccaggtgctg ccgcgcatga cggcggggga ctggctgggc
     7141 gcctactcgc tgtccgagcc cggcagcggc tccgacgcgg ccggcctgtc cacgcgcgcg
     7201 cgcctggacg gcgagcactg ggtcgtcgac ggcgtgaagg cctgggtgac ccaccagggc
     7261 atcgcgcagg tctacgtgct gatgtgccgc accggcgagc acaagacgcg tggcgtgtcc
     7321 gcgctgctgg tgcccggcga cacgccgggc ctgtccttcc ccgccgtgga gcgcaagatg
     7381 ggcatgcgcg cctcgccgac cggccagctc gtgttcgacg gcgcccgcgt gcccgccgcg
     7441 aacctgctgg gcgaggaggg cgagggcttc aagatcgcca tggccgccct ggacggcggc
     7501 cggctgggca tcgccgccgc gtccgtcggc ctggcgcagc gctgcctgga cgagtcggtc
     7561 gcctacgcgc gccagcgcac ccagttcggc cgccccatcg ccgacttcca gggcgtggcg
     7621 ttcatgctcg ccgacatggc caccgccgtg gaggccgcgc gcgccctgta cctggaggcc
     7681 gccgaccgcc gcgacctggg cctggcctac gccaggctgg cggccatggc gaagctctcg
     7741 gccagcgaca ccgcgatgaa ggcggccgtg gacgcggtgc aattgcacgg cggctacggc
     7801 tacaccgagg actacccggt ggagcggctc atgcgcgaag ccaagatgct gcagatcgtg
     7861 gagggcacca accaggtgca gcgcatggtg atctcccggg acctgctgcg cgacggcgcc
     7921 tgagcggtgc ctgcgacccc gcacggcccg cgcgaccgga ccaccgtccg gcgggcgctc
     7981 ggggcgggag cggccgtctg ggcggcttgc ctggcgctgt ggacgctgct gggggtggcg
     8041 ggcacgcccg gcctcgagca cgtgccgttg tacgcggccc tgggcgcccc gttcgcgggc
     8101 atcgccgcgg cgctgtggct gtggctggcc atcctgctcg acgtgctggc gggggagcgg
     8161 cccagccggc ggcgctggct gtgggcggcc gggctgacgg tggtgacggc gttcgtgctg
     8221 ccggtgatgc tcagcgtcgc gttcgcgatc tgaccgtgcc gcctcgctag ggttgggcgc
     8281 atgcccgagt tcacctgcgc cgagtgcggc agcgccttca gcctgccgga cgcggtcctg
     8341 gccaagtacc ccggctggac gccgcgccag tgccggtcct gccgggacgc cggcggcggc
     8401 gccgcggcca aggcgcccac agccaaggcg cccacagcca aggcgcccgc ggcggcgcgg
     8461 ggcgggcgca agcgccgcgc cgtggaggag aacctgccgg tcgccgaggt gctggcgcgc
     8521 cacgccggcg gcccgcagga cggcgtgttc accgacg
//
LOCUS       DATMKC010000010        18215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_164667, whole genome shotgun sequence.
ACCESSION   DATMKC010000010 DATMKC010000000
VERSION     DATMKC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 18215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18215
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164667"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VNU01_00300"
     CDS             <1..444
                     /locus_tag="VNU01_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31088.1"
                     /translation="ALVRVILKHCNNQRSNDFYIGYIGADYSGWCREAGFKNVEVLPL
                     TGRASAQVVQTLRDVVDDGLPGYHEPADEPFVIAKGTPPTFRDALLAWEERHQERVRL
                     WGGGSVPSRPSRSRGSDGTTDSHGVAIRGDPCQIDDSDAASAALR"
     gene            complement(541..1770)
                     /locus_tag="VNU01_00305"
     CDS             complement(541..1770)
                     /locus_tag="VNU01_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWH31089.1"
                     /translation="MDTGDTAWMLMSTALVLFMTPGLAFFYGGMVRGKSALNMLMMSF
                     SAMGLLAVLWALGVFSLAFGSFGDAGWLGSLEFAGLRGVGMEPGLGLTIPFVLFAAYQ
                     MTFAVITPALISGAVAERMKFGAYLLFIGVWLVVVYAPVAHWVFGGGWLADMGALDFA
                     GGAVVHINAGAAALALVLVLGRRRGWPQEAMHPHSLPLTLLGTGMLWFGWFGFNAGSA
                     LAANGLAAQALMNTFLAASAAMLGWMLAERLKTGHATTLGAASGAVAGLVAITPCAGF
                     VEPLASLAIGFVTGAVCLFAVQLKFRLGYDDSLDVVGVHLVGGLIGSVLLGLFATSSV
                     NELVTREGLLYGGGGELLGIQAVASAVTLAFSFVVSLLLAKAIDLVMGIRVTPEQEQE
                     GIDQSQHAEAAYALDPS"
     gene            complement(1865..3820)
                     /locus_tag="VNU01_00310"
     CDS             complement(1865..3820)
                     /locus_tag="VNU01_00310"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HWH31090.1"
                     /translation="MSGPVGEVIWTPSPERVSASAMERFRQGAPGAPADWEALYAWSV
                     EDLPGFWGHVWDELGVIASTPYDRVMGEPRMPGTEWFPGARLNFAEHLLRRDDESPAV
                     IATGEGREDRVVSARELRALVARAQTGLRALGVGVGDRVAALVPNALETLVLMLATTA
                     SGAIWSSCSPDFGPMGVVDRFGQIRPKVLVTADGYRYNGNAFSLRDKVEPVLARIGEI
                     EHVVVFDFVGQGLAGLDRPATTFAELAADGPDVPEFVPLPPDHPLYIMYSSGTTGVPK
                     SIVHGAGGTLVKHLVEHRLHSDVGPGDRIFWFTTCGWMMWNWLVSGLAAGACVVLYDG
                     SPTHPGPEALWRMAARTRVTHFGTSPKFLAACEHAGLVPRDAADLSKVRALLSTGSPL
                     NPEQFDWVYANVPADPGRPDLQLASISGGTDLIGCFAAGNPLLPVRRGELQSRGLGMA
                     VEAFAPDGTPVYGEKGELVCTKPFPSMPVGFWDDPDGERYRKAYFDAFPGVWTHGDFV
                     EIRPSGGVVIYGRSDTTLNPGGVRIGTAEIYRAIEGMPEVADAIVVGRPVDGDVEVVL
                     CVVLGEGSALDEELTGRIRATIRAATTPRHVPAHVFAVPEVPYTISGKKVEKAVRSMI
                     TGEPVDNRDALANPGALDAYAALPFPN"
     gene            complement(3821..5110)
                     /locus_tag="VNU01_00315"
     CDS             complement(3821..5110)
                     /locus_tag="VNU01_00315"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWH31091.1"
                     /translation="MSDHARIEIDGQVFEAPMIVGTEEERAIDIGKLRAGTGLITYDP
                     GFANTGSTRSAITYLDGEAGILRYRGYPIEELAQHSTFLETSYLLIYGELPTRDELES
                     FTHQITHHTLLHEDMRRFFDAFPKNAHPMAILSAATSALSTFYQDSHDPFDPDDVEIS
                     TYRLMAKLPTVAAFAYKKSIGQPYVYPQNRLGFAENFLHMMFAVPAETYEVDPDVADA
                     LNLLLVLHADHEQNASTSTVRLVGSTHANLFASIAAGITALWGPLHGGANQAVVEMLE
                     EILADGGNSAKYIAKAKDRNDPFRLMGFGHRVYKNYDPRARIIKQTADRIIGKLVGDD
                     PLFQVARELEEAALKDDYFVEKKLYPNVDFYSGLIYRAMGLPLNMYPVLFALGRLPGW
                     VAQWREMITDDETRIGRPRQVYVGASQREYTKIHDRG"
     gene            5290..7071
                     /locus_tag="VNU01_00320"
     CDS             5290..7071
                     /locus_tag="VNU01_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HWH31092.1"
                     /translation="MSGGQADALPAGASRRLRGCLEQLYPGADIDRLLRRLEGMAAAF
                     QVERRMLGARPRETAALFDERDVLLITYGDQVRRDGSPPLRTLHDFLRRRFGDTLTGV
                     HLLPFFPSTSDGGFAVADYEAVDPELGDWDDIRRFGRDFRLMVDAVFNHTSASHPWFT
                     GWCAGDPAYADFYVTADPGTDLSGVTRPRQHPLLTKFDHADGERWVWTTFSADQADLN
                     FANPEVLLATTEVLLDYVANGAGIIRLDAVAYLWKEVGTSCIHHTGTHTIIRLWRALL
                     DAVAPGTLIVTETNVPHLENISYFGDGHDEAHVVYQFPLAPLVLSAFHLADAGTLTDW
                     AAGLSTPSEETTFLNFLGSHDGIGVRPVEGILTHAEIQQLVALATAHGGGVSYKANPD
                     GTLSPYELNSVYFDALTEVDSRESMEVQVRRFLSAQSILLALPGVPAIYVQALLGSRN
                     WVEGVELTGHLRSINRQKFPLEELERELDDPRTLRHQVSTRLAERIRTRTGEPAFHPN
                     AAARVLQAGSKVFAIQREAVDGSSAVVCLHSLSGRREPVVVGPDEGLSVAGEFRDLCA
                     ERGASTDAAGVLHVELEPYGVAWLKRL"
     gene            complement(7128..8099)
                     /locus_tag="VNU01_00325"
     CDS             complement(7128..8099)
                     /locus_tag="VNU01_00325"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HWH31093.1"
                     /translation="MSATPRNKRMEGPLADWFARRTFHTDDFDIADLRRRKEEAGTTV
                     SVVLPSFNEADTVAGVIQAVRELEGTLIDEIVLIDGGSGDDTADIAAAAGARVHQDAD
                     VLPDLGPTLGKGDALWRSLTVTTGDIVVFVDTDIRNPDPRFVWGLLGPLLVEPQVQLV
                     KAFYERPVQMGDVLHATGGGRVTELMARPLLNLFWPELAGLVQPLSGEYAGRRELLES
                     IPFFTGYGVEIGMLVDTLAGSGIDAIAQVDVSERVHRNQAMDALSRMAYGIVQVAIAR
                     LADEGRLPRELAEASERYVQFVRDERGRIQPAEREVPIIERPPLRQA"
     gene            complement(8125..8745)
                     /locus_tag="VNU01_00330"
     CDS             complement(8125..8745)
                     /locus_tag="VNU01_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWH31094.1"
                     /translation="MNSDFRNRVLLPILLPLLAGAGIIGFAFALSRVLLAVSEAASNF
                     IALFVALYLLAVAGMVTARPRISSKALGVGLTIGLLSVGVAGVLGAQAGMRELHHAEE
                     EPAEEPAEAPAAAELPEGAHVFVAVDIDFSQAPETIPAGEQVIAIVNEGGAPHNVVFE
                     GTDVKVEAAGGESASDTYELEPGTYTYFCDVPGHRSSGMEGTVTVE"
     gene            complement(8788..9639)
                     /locus_tag="VNU01_00335"
     CDS             complement(8788..9639)
                     /locus_tag="VNU01_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWH31095.1"
                     /translation="MSPADPLRWQPHPDAWLLGILLLGGYFYALAAWGPRLAPGRTPA
                     SRRHKQAFVAGVLTLWIASDYPIHGLSEHLFSAHMLQHTIYSLVSAPLLILGVPGWLW
                     RRLLSPAPVRAVWRTATRPLVALAIFNGWIAVYHAPFMVNASVHSEIAHFAMHIAWVL
                     ASLVMWWPVLSPLPELPHLSPTGRMLFLFAQSIVPTVPASFLTFSHAPIYAAYDTATR
                     AFGLSAIDDQQIAGLLMKIGGGFLIWGVITVLFFRWATQERTGGPDPLYWRDLEAGLE
                     PAGQGAR"
     gene            complement(9644..9943)
                     /locus_tag="VNU01_00340"
     CDS             complement(9644..9943)
                     /locus_tag="VNU01_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWH31096.1"
                     /translation="MAAEHPAHDEHDSHPGVGQYIEIGAILALLTAIEVSLFFADIAS
                     AVEVPALLFLTVLKFVLVILWFMHLRFDHKVFRRLFFTGVALAGVVFSVVLVISG"
     gene            complement(9959..10585)
                     /locus_tag="VNU01_00345"
     CDS             complement(9959..10585)
                     /locus_tag="VNU01_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWH31097.1"
                     /translation="MADVTAATEAHSDAGGTGEHHTSMGLSNNKMGMWALIGSECLFF
                     GALISTYLMYLDRTNGGPGPEIFDIPFTSVSTFILLMSSLSMVLALAAIQEGDMRKFR
                     TWTLGTALLGSTFLAGQFYEFTVFVAEGMKLDTSPFSSAFFVLTSFHGFHVAFGILML
                     VSLWAMSLSGRVKPVVHAPLVENIGLYWHFVDIVWIVIFTVVYLIPAG"
     gene            complement(10585..12579)
                     /gene="ctaD"
                     /locus_tag="VNU01_00350"
     CDS             complement(10585..12579)
                     /gene="ctaD"
                     /locus_tag="VNU01_00350"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWH31098.1"
                     /translation="MTAIAEKPSTGGSLFRRPRATTGPASWFTTVDHKKIGILYFFTA
                     MFFFLVGGLEALLIRIHLAGPNSSVLTYDQYNQLFTMHGLTMIFFVVMPLGSAFMNYL
                     LPLQIGARDVAFPRLNGFSYWVFLAAGIFTYSSLFLGGMPDGGWFGYAPLSLLGGDPI
                     GQTVGTAGAEMSRMIFYSIGLQIAGIASLASAINFIVTTFNMRAPGMTLLRMPVFTWM
                     TLVVAFLLLFALPIIGVALWQLMFDGAFGANFFRPSAGGDPILWQHMFWLFGHPEVYI
                     LILPAFGIVSEVLPTFSRKPLFGYTAVVFSGIAIGFMGWGVWAHHMFAVGLGPVANAA
                     FGLTTMFIAVPTGIKIFNWMGTMWGGKLRFPTPMLFAIGLVSLFTIGGLSGVTHGVVP
                     HDYQQTDTYYVVAHFHYVLFGGALMGFFSGAYYWFPKVTGKFMNDKLGKLHFWIFFVG
                     MNLTFGPMHIVGLNGMPRRTATYPEGMGWELMNRIETAGALLLAVGVLVYLYNLATSL
                     RGPKTAGADPWDARTLEWLTSSPPPAHNFDEIPIVQERDEAWFRKYGSTHEGEPEKVP
                     AGASQDHRHEPGQDHAADVPAEAAEPDPAHHEPVGESGGHAAIHMPDPSWYPLIVAAG
                     MPIGGWGVIFDSTILIALGGLVIFGGIAGWVLEPSAEETH"
     gene            complement(12623..13744)
                     /locus_tag="VNU01_00355"
     CDS             complement(12623..13744)
                     /locus_tag="VNU01_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWH31099.1"
                     /translation="MRAGRPRRSGLIGWALLAVAMLLLAGCANADLPQDALSPEGPVA
                     RQQDQLWDIVYPIAIGVFVFVQLGLLVVMWRFRSRGDDTAIPHQLHGNTKLELLWTII
                     PALILAIIAVPTTRTIFALADEPDDALQVRVIGKQFWWEFEYTGEEGQGVVTANELHI
                     PTGRPVFLSMEALSPQIPDSVVPGEESDERNPQSLDGVIHSFWVPKLAGKQDVVPGHV
                     RTMYIEADNPGRYEGQCAEFCGIGHAVMRFEVVAHDPGDFEEWLTAQAEPAFDGQSGL
                     AAQGKELFETAGCIACHAINGYETSGGGAAGVRVGPNLTHFASRSIFAGGLFDNSDAE
                     QLAAWLRDPQSVKQGAQMPNLGLSEEQIEALVAYLQSLK"
     gene            13926..14675
                     /locus_tag="VNU01_00360"
     CDS             13926..14675
                     /locus_tag="VNU01_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31100.1"
                     /translation="MRGTAETDVETGLRRVVVGFRAAAVLWMALLLLVGGLPPGLRPA
                     LAAAGVLGLAGAWAFLTALLAAARPDALRSWAWLAVDTLVAAVVVLGAVIVAAAGSFA
                     VAVAVPVAAFAGGYPFSAVLLGGWARGVPGGLAAASVLSAATVARDAAGARGWTLEDS
                     LPATLVYLAGALVTAWGVRVLRRQDAERRTALLALAEERAATARERERAETAAHLHDS
                     VLQTLALVQRRAEDPAAVRALAREQERELRA"
     gene            14757..15080
                     /locus_tag="VNU01_00365"
     CDS             14757..15080
                     /locus_tag="VNU01_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH31101.1"
                     /translation="MRVDVVVSGDRALDEPLAALVSAAAEAVRNAARHSGAPEVAVYV
                     ELGAQPVVFVRDRGRGFDPDAVPPDRRGIAESVRGRLRRHGGQALLRTAPGQGTEWEL
                     RLGAS"
     gene            15077..15721
                     /locus_tag="VNU01_00370"
     CDS             15077..15721
                     /locus_tag="VNU01_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWH31102.1"
                     /translation="MTREPATPEDLRRAAAAVVAALWPHRDGDWQVRAGELDWTCLDT
                     LGHTVDALRFYAANLATRTRVRRAYPWAEAFGGTPEVWLLALEATAELLAVVAETTAD
                     DDRAFHPAGLADAEGFLAMGCDEVLVHGADVAQGLGVALEAPDDVAALVLARLFPWVE
                     PGDGERAWDLLRWANGRTELPGRARLGPDWWWHCAPLDEWDGSGPRRRSEPPPR"
     gene            15730..16365
                     /locus_tag="VNU01_00375"
     CDS             15730..16365
                     /locus_tag="VNU01_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH31103.1"
                     /translation="MALSVFLVDDHRLFRSGIRAELAGRLHIVGEAGTVDEAVEGIVR
                     LDPTVVLLDVHLPDGGGPAVLARCRAAGYGGQFLALSASDDPKDVVEVIRAGARGYVT
                     KTVDAAELVDAVRRVAGGDAVFSPKLAGFVLDAFSSIPAGQVDADLARLSPREREVLQ
                     LVARGYTYREIAERLVLSVKTIESHVSAALRKLQLSNRRDLARWAGERGID"
     gene            complement(16362..17690)
                     /locus_tag="VNU01_00380"
     CDS             complement(16362..17690)
                     /locus_tag="VNU01_00380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWH31104.1"
                     /translation="MSTFSDLGVSPKIIAALAKRGIDAPFPIQALTIADAMTGRDLCG
                     RAPTGSGKTLAFGVPVASLARKGGPRKPSALILVPTRELAAQVAETIAPLAQARGLNV
                     ASFYGGTSIGKDRQRLERGVDIAVACPGRLEDLIDRNVVNLSNVRLVVLDEADRMADM
                     GFLPAVKRLLDQTSPERQTLLFSATLDGDVDVVIRRYQRDPARHELAASAEDDGDVRH
                     LFWTAGSSDRVRVTRDIVGRSSPAIVFTRTKHGADRVAKQLVREGVRAVAIHGDRSQG
                     QRQRALADFTAGKASVLVATDVAARGIHVDKVGVVVHFDPAGTDKDYVHRSGRTGRAG
                     ADGIVVTLVSPEKRKDVQSLQRALGMRQPIQAPALDQLADGVPMPAPVPIDRTERAER
                     PRAAAPGRNPRQGQRQGASQRQGQPRAKGQGQGQGQGSGRPGGQRRRRAA"
     gene            18023..>18215
                     /locus_tag="VNU01_00385"
     CDS             18023..>18215
                     /locus_tag="VNU01_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31105.1"
                     /translation="MNELLRQPLSRRILVTAAIGSLADWTLFAALVAVVAGLTGGSVF
                     AVALVTGARILPSVLLGPLV"
BASE COUNT         2606 a         6363 c         6681 g         2565 t
ORIGIN      
        1 gccctggtca gggtaatcct gaagcattgc aacaatcaac gatcgaacga tttttacatc
       61 ggctacatcg gcgcggacta ctccggctgg tgccgcgaag ccggcttcaa gaacgtcgag
      121 gtgctgccgc tcacgggacg ggcgagcgcc caagtcgtcc agacattgcg ggacgtggtc
      181 gatgacggtc tgcccggata tcacgaacct gcggacgagc cgttcgtcat cgccaaaggc
      241 accccgccga cgttccgtga cgccctgctc gcatgggagg aacgccacca ggagcgggtc
      301 cgcctctggg gcggaggatc ggttccgtct cggcctagcc gttctcgcgg ctccgatgga
      361 acaacggact cccacggcgt ggcgattcgt ggcgacccat gccagattga tgactccgat
      421 gccgcgtcag cagcccttcg ctaggcgcct aggttccaca cgggggcaac gcggtacggc
      481 cggggaaggt ggcactgcga tagagcggta tgtaagccag atcctccgac ttcctctcgg
      541 tcaggagggg tcgagggcgt aggccgcctc ggcgtgctgg ctctggtcga tgccctcctg
      601 ctcctgctcg ggcgtgaccc ggatgcccat gacgaggtcg atcgccttgg ccaagagcag
      661 cgacacgacg aagctgaagg ccagcgtcac ggcgctcgcg accgcctgga tgcccagcag
      721 ctccccgccg ccgccgtaca gcagcccctc gcgcgtgacc agctcgttca ccgaggaggt
      781 cgcgaacagg cccagcagca cggagccgat cagcccgccc accaggtgca cgccgaccac
      841 gtcgagcgag tcgtcgtagc ccagccggaa cttgagctgt accgcgaaca ggcagaccgc
      901 gccggtgacg aagccgatgg ccagcgacgc caggggctcc acgaacccgg cgcacggcgt
      961 gatcgcgacc agcccggcca ccgcgccgga cgcggcgccc agcgtggtcg cgtgcccggt
     1021 cttcagccgc tccgccagca tccagcccag catggcggcg gaggcggcca ggaaggtgtt
     1081 catcagcgcc tgcgcggcca gcccgttggc ggccagcgcc gagccggcgt tgaagccgaa
     1141 ccagccgaac cacagcatcc ccgtgcccag cagcgtcagc ggcaggctgt gcggatgcat
     1201 ggcctcctgc ggccagccgc ggcgcctgcc gagcaccagc accagcgcga gcgcggccgc
     1261 gcccgcgttg atgtgcacga ccgcgccgcc cgcgaagtcc agcgcgccca tgtcggcgag
     1321 ccagccgccg ccgaacaccc agtgcgcgac gggggcgtag acgaccacca gccacacgcc
     1381 gatgaacagc aggtacgcgc cgaacttcat gcgctcggcc acggcgccgc tgatcagcgc
     1441 gggcgtgatc accgcgaagg tcatctggta ggcggcgaac agcacgaacg ggatggtcag
     1501 ccccagcccc ggctccatgc cgacgccgcg cagccccgcg aactccaggc tgccgagcca
     1561 ccctgcgtcg ccgaagctgc cgaacgccag gctgaacacg cccagcgccc acagcaccgc
     1621 cagcaggccc atggcgctga agctcatcat gagcatgttg agcgcgctct tgccgcgcac
     1681 catgcccccg tagaagaagg cgaggcccgg ggtcatgaac agcaccaggg ccgtggacat
     1741 gagcatccac gcggtatcgc cggtgtccat ggcaactccc accagtcgtc gtgacacacg
     1801 aagtcagcgg cgagcgtgca gggtgactgt tacgaggcgg tttccccgcc gtttcccccc
     1861 tggatcagtt cgggaacggc agggcggcgt aggcgtccag agcgccgggg ttggccagcg
     1921 cgtcgcggtt gtcgacgggc tcgccggtga tcatggagcg cacggccttc tcgaccttct
     1981 tgccgctgat cgtgtacggc acctcgggca cggcgaacac gtgcgcgggc acgtgccgcg
     2041 gggtggtggc cgcccggatg gtcgcgcgga tgcggccggt cagctcctcg tccagcgcgc
     2101 tgccctcgcc gagcaccacg cacagcacca cctcgacgtc gccgtccacg ggccggccga
     2161 ccacgatggc gtcggcgacc tccggcatcc cctcgatcgc gcggtagatc tccgcggtgc
     2221 cgatgcgcac accgcccggg ttgagcgtcg tgtcgctgcg gccgtagatc accaccccgc
     2281 cggacgggcg gatctcgacg aagtcgccgt gggtccacac gcccgggaac gcgtcgaagt
     2341 aggccttgcg gtagcgctcg ccgtcggggt cgtcccagaa cccgaccggc atcgacggga
     2401 acggcttggt gcagaccagc tcgcccttct cgccgtacac gggggtgccg tccggcgcga
     2461 acgcctccac ggccatgccc aggccgcgcg actgcagctc gccgcggcgc acgggcagca
     2521 gcgggttgcc ggccgcgaag cagccgatca ggtccgtgcc gccgctgatg gacgcgagct
     2581 gcaggtccgg ccggccggga tcggccggca cgttggcgta cacccagtcg aactgctccg
     2641 ggttcagcgg cgagccggtg gacagcagcg cgcgcacctt cgacaggtcg gcggcgtcgc
     2701 gggggaccag gccggcgtgc tcgcacgcgg ccaggaactt ggggctggtg ccgaagtgcg
     2761 tcacgcgggt gcgggccgcc atgcgccaca gcgcctccgg ccccgggtgg gtcgggctgc
     2821 cgtcgtacag cacgacgcag gcgccggcgg caaggccgga gaccagccag ttccacatca
     2881 tccagccgca ggtggtgaac cagaagatcc tgtcgcccgg ccccacgtcg ctgtgcaggc
     2941 ggtgctcgac caggtgcttg accagcgtgc cgccggcgcc gtggacgatg ctcttgggca
     3001 ccccggtcgt gcccgagctg tacatgatgt acagcgggtg gtcgggcgga agcggcacga
     3061 actcgggcac gtcggggccg tcggcggcga gctcggcgaa cgtcgtggcc gggcggtcca
     3121 ggccggcgag cccctggccg acgaagtcga acaccacgac gtgctcgatc tcgccgatgc
     3181 gcgccaggac cggctccacc ttgtcgcgca ggctgaacgc gttgccgttg tagcggtagc
     3241 cgtccgcggt cacgagcacc ttgggccgga tctgcccgaa gcggtcgacc acgcccatcg
     3301 ggccgaagtc gggggagcac gacgaccaga tcgcgccgct ggccgtggtg gccagcatca
     3361 gcaccagcgt ctccaaggcg ttgggcacca gcgccgcgac gcggtcgccc acgccgacgc
     3421 ccagggcgcg caggccggtc tgcgcccgcg cgacgagggc gcgcagctcc cgggcgctca
     3481 cgacgcggtc ctcgcgaccc tcgcccgtgg cgatcaccgc gggcgactcg tcgtcgcgcc
     3541 gcaggaggtg ctcggcgaag ttcagccgcg cgccggggaa ccactcggtg cccggcatgc
     3601 ggggctcgcc catgacgcgg tcgtacgggg tgctcgcgat cacgcccagc tcgtcccaga
     3661 cgtggcccca gaagccgggc aggtcctcga cggaccaggc gtacagcgcc tcccagtccg
     3721 cgggtgcgcc gggcgcaccc tggcggaagc gctccatcgc gctggccgag acccgttcgg
     3781 gcgacggcgt ccagatgacc tctccgacgg ggccgctcac ctagccgcgg tcgtggatct
     3841 tggtgtactc gcgctggctg gcgcccacgt agacctggcg cggccgcccg atgcgggtct
     3901 cgtcgtcggt gatcatctcg cgccactgcg cgacccagcc gggcaggcgg cccagcgcga
     3961 acagcaccgg gtacatgttc agcggcaggc ccatcgcccg gtagatcagg ccggagtaga
     4021 agtccacgtt ggggtacagc ttcttctcga cgaagtagtc gtccttcagg gccgcctcct
     4081 ccagctcgcg cgcgacctgg aacagcgggt cgtcgccgac cagcttgccg atgatgcggt
     4141 cggcggtctg cttgatgatc cgcgcgcgcg ggtcgtagtt cttgtagacg cggtgcccga
     4201 agcccatgag tcggaacggg tcgttgcggt ccttggcctt ggcgatgtac ttggcgctgt
     4261 tgccgccgtc ggccaggatc tcctccagca tctccaccac ggcctggttc gcgccgccgt
     4321 gcaggggccc ccacagcgcg gtgatgcccg ccgcgatgga ggcgaacagg ttcgcgtgcg
     4381 tgctgccgac caggcgcacc gtggacgtcg aggcgttctg ctcgtggtcg gcgtgcagga
     4441 ccagcagcag gttcagcgcg tcggcgacgt ccgggtcgac ctcgtaggtc tcggccggga
     4501 ccgcgaacat catgtgcagg aagttctccg cgaagccgag acggttctgt gggtagacgt
     4561 agggctggcc gatggacttc ttgtacgcga acgccgccac ggtgggcagc ttggccatca
     4621 ggcggtacgt ggagatctcg acgtcgtcgg ggtcgaaggg atcgtgactg tcctggtaga
     4681 aggtcgacag cgccgaggtt gccgccgaga ggatcgccat ggggtgcgcg ttcttgggga
     4741 aagcgtcgaa gaagcggcgc atgtcctcgt gcagcagcgt gtgatgggtg atctggtgag
     4801 tgaacgactc gagctcgtcc ctggtgggca gctccccgta gatcagcagg tagctggtct
     4861 ccaggaacgt ggagtgctgc gcgagctcct cgatcgggta gccccggtag cgcaggatcc
     4921 ccgcctcacc gtccaggtag gtgatcgcgc tgcgcgtgga gccggtgttg gcgaatcccg
     4981 ggtcgtaggt gatcagcccc gtgccggcgc ggagcttgcc gatgtcgatg gcgcgctcct
     5041 cctcggtacc gacgatcatc ggtgcctcga agacctggcc gtcgatctcg atgcgtgcgt
     5101 ggtcgctcat gccctcttcc cctgtgggcc caccgcgtcc ggggtctccg gccggcgcga
     5161 gccctggtcg tctacggctg cgggtcggca gtccggcgcc gttgccgcgc tgcaccacat
     5221 gcaagcctac tgcgtcctcc ggccgctcaa cgtagcccga caccgcccgt gccggggacg
     5281 ggggcgcccg tgagcggggg tcaggcggat gccctgccgg cgggcgcctc ccggcgcctg
     5341 cgcggctgcc tggagcagct ctacccaggg gcggacatcg acagactcct gcggcggctg
     5401 gagggcatgg cggcggcgtt ccaggtggag cggcggatgc tgggggccag gccgcgcgag
     5461 accgcggcgc tgttcgacga gcgcgacgtg ctgctgatca cctacggcga ccaggtgcgc
     5521 cgcgacggga gcccgccgct gcgcacgctg cacgacttcc tgcgccggcg cttcggcgac
     5581 acgctgaccg gggtgcacct gctgccgttc ttcccgtcca cctccgacgg cggcttcgcc
     5641 gtggccgact acgaggccgt ggacccggaa ctgggggact gggacgacat ccgccgcttc
     5701 gggcgcgact tccgcctcat ggtcgacgcg gtgttcaacc acacctccgc gtcgcacccg
     5761 tggttcacgg gctggtgcgc gggcgacccc gcgtacgcgg acttctacgt gaccgccgac
     5821 ccgggcaccg acctgtccgg cgtgacgcgg ccccgtcagc acccgctgct gaccaagttc
     5881 gaccacgccg acggggagcg ctgggtctgg accacgttct cggcggatca ggcggacctc
     5941 aacttcgcga acccggaagt gctgctggcc accaccgagg tcctgctgga ctacgtggcc
     6001 aacggtgccg gcatcatccg cctcgacgcg gtggcctacc tgtggaagga ggtgggcacc
     6061 agctgcatcc accacaccgg cacgcacacg atcatccgcc tgtggcgcgc gctgctggac
     6121 gccgtggccc ccgggacgct gatcgtcacg gagacgaacg tgccgcacct ggagaacatc
     6181 tcctacttcg gcgacgggca cgacgaggcg cacgtggtgt accagttccc gctggcgccg
     6241 ctggtgctgt cggcgttcca cctggccgac gccggcacgc tgacggactg ggcggccggg
     6301 ctgtccacgc cgagcgagga gaccacgttc ctcaacttcc tggggtccca cgacggcatc
     6361 ggggtgcgcc ccgtggaggg catcctcacg cacgccgaga tccaacagct cgtggccctg
     6421 gcgaccgcac acggcggcgg cgtgtcctac aaggccaacc ccgacgggac cctgagcccc
     6481 tacgagctca actcggtgta cttcgacgcg ctgacggagg tggacagccg cgagtcgatg
     6541 gaggtgcagg tgcggcggtt cctgtcggcg cagtccatcc tgctggcgct gcccggggtc
     6601 ccggcgatct acgtgcaggc gctgctgggc agccgcaact gggtcgaggg cgtggagctg
     6661 accgggcatc tccggtcgat caaccggcag aagttcccgc tggaggagct ggagcgcgag
     6721 ctcgacgatc cccgcacgct gcgccatcag gtgtccacgc gcctggccga gcgcatccgc
     6781 acgcgcaccg gcgagcccgc gttccacccg aacgccgcgg cgcgcgtcct gcaggccggc
     6841 tccaaggtgt tcgcgatcca gcgggaggcg gtcgacggct cctcggcggt ggtgtgcctg
     6901 cactcgctgt ccggccggcg cgagcccgtg gtggtcgggc cggacgaggg cctgtcggtg
     6961 gcgggggagt tccgggacct ctgcgcggag cggggcgcct cgaccgacgc cgccggcgtg
     7021 ctgcacgtgg agctggagcc ctacggcgtc gcctggctca agcgcctctg aacccggggt
     7081 gtgtcgactg gatcatcgtc acattgactg agtcgacaca ccccgggtca cgcttggcgg
     7141 agcggggggc gttcgatgat ggggacctcg cgctcggcgg gctggatgcg gccgcgctcg
     7201 tcgcgcacga actgcacgta gcgctcgctg gcctcggcca gctcgcgcgg cagccggccc
     7261 tcgtcggcca ggcgggcgat cgccacctgc acgatgccgt aggccatgcg gctcagcgcg
     7321 tccatcgcct ggttgcggtg cacgcgctcg gagacgtcca cctgcgcgat cgcgtcgatg
     7381 ccggagccgg ccagcgtgtc caccagcatc ccgatctcca cgccgtagcc ggtgaagaac
     7441 gggatggact ccagcagctc ccgccgcccg gcgtactcgc cggacagcgg ctgcaccagc
     7501 cccgccagct ccggccagaa caggttcagc agcggccgcg ccatgagctc ggtgacgcgg
     7561 ccgccgccgg tggcgtgcag cacgtcgccc atctgcacgg gacgctcgta gaacgccttg
     7621 accagctgca cctgcggctc caccagcagg gggccgagca gcccccagac gaagcgcggg
     7681 tccgggttgc ggatgtcggt gtccacgaac acgacgatgt cgccggtggt cacggtgagg
     7741 ctgcgccaca gcgcgtcgcc cttgcccagc gtggggccca ggtccggcag cacgtcggcg
     7801 tcctggtgca cgcgggcgcc cgccgcggcg gcgatgtcgg cggtgtcgtc gccggagccg
     7861 ccatcgatga ggacgatctc gtcgatcagg gtgccctcga gctcgcggac ggcctggatg
     7921 acgccggcca cggtgtcggc ctcgttgaac gatggcagca ccacgctcac ggtggtgccg
     7981 gcctcctcct tgcggcggcg gaggtcggcg atgtcgaagt cgtcggtgtg gaacgtccgg
     8041 cgggcgaacc agtcggccag ggggccctcc atgcgcttgt tgcgtggtgt ggcgctcacg
     8101 cgctgccctc ccgcatcgtg cgccctactc gaccgtgacc gtgccttcca tgcccgagga
     8161 gcggtgcccg ggaacgtcgc agaagtaggt gtaggtcccc ggctccagct cgtaggtgtc
     8221 gctcgcggac tcgccgccgg cggcctcgac cttcacgtcg gtgccctcga agaccacgtt
     8281 gtgcggtgcg ccgccctcgt tgacgatcgc gatcacctgc tcgccggccg ggatcgtctc
     8341 cggcgcctgc gagaagtcga tgtcgaccgc cacgaacacg tgggcgccct cgggcagctc
     8401 ggcggcggcc ggcgcctccg ccggctcctc ggcgggctcc tcctcggcgt ggtgcagctc
     8461 ccgcatgccg gcttgggcgc ccagcacgcc ggccacgccg acggacagca gcccgatggt
     8521 cagcccgacg ccgagcgcct tggagctgat gcgcgggcgg gcggtcacca tgccggcgac
     8581 ggccagcagg tacagggcca cgaacagcgc gatgaagttc gacgccgcct cgctgacggc
     8641 gagcagcacg cgcgacagcg cgaacgcgaa cccgatgatg cccgcccccg ccagcagcgg
     8701 caggaggatc gggagcagga cccggttgcg gaagtcggag ttcatggctt gcttcgtccc
     8761 tttctggact gcccggcggt gcagcggtta gcgggcgccc tggccggccg gctccaggcc
     8821 ggcctccagg tcgcgccagt acaacgggtc ggggccgccg gtgcgctcct gcgtcgccca
     8881 gcggaagaac agcaccgtga tgacgcccca gatcaggaac ccgccgccga tcttcatcag
     8941 caggccggcg atctgctggt cgtcgatcgc gctcaggccg aacgcgcgcg tggccgtgtc
     9001 gtaggcggcg tagatcgggg cgtgggagaa cgtcaggaag ctcgcgggca ccgtcggcac
     9061 gatcgactgc gcgaacagga acagcatccg gccggtgggc gacaggtgcg gcagctccgg
     9121 cagcggcgac agcacgggcc accacatcac cagggaggcc agcacccacg cgatgtgcat
     9181 ggcgaagtgc gcgatctcgc tgtgcacgct cgcgttgacc atgaacggcg cgtggtacac
     9241 ggcgatccag ccgttgaaga tcgccagcgc caccagcggc cgggtggcgg tgcgccagac
     9301 ggcccgcacc ggtgcggggg acagcagcct gcgccacagc cagcccggca cgcccaggat
     9361 cagcagcggc gccgagacca gcgagtagat ggtgtgctgg agcatgtgcg cgctgaacag
     9421 gtgctcggac aggccgtgga tggggtagtc ggaggcgatc cacagcgtca gcacgccggc
     9481 gacgaacgcc tgcttgtgcc ggcgcgacgc cggcgtccgc ccgggcgcca ggcgcgggcc
     9541 ccacgcggca agcgcgtaga agtacccgcc cagcagcagg atcccgagca gccacgcgtc
     9601 gggatggggc tgccagcgga gcgggtcggc ggggctcatc gcgctagccc gagatcacca
     9661 ggacgacgga gaacaccacg ccggccaggg ccacgccggt gaagaacagc cgccggaaca
     9721 ccttgtggtc gaagcgcagg tgcatgaacc acaggatcac cagcacgaac ttcagcacgg
     9781 tcaggaagag cagcgccggg acctccaccg cggaggcgat gtcggcgaag aacagcgaca
     9841 cctcgatcgc ggtgagcagc gccaggatcg cgccgatctc gatgtactgg ccgacgcctg
     9901 ggtgggagtc gtgctcgtcg tgcgcagggt gttcagcagc catgggtctc cgcgctcctt
     9961 agccggccgg gatgaggtag acgaccgtga agatcacgat ccagacgatg tcgacgaagt
    10021 gccagtacag gccgatgttc tccaccagcg gcgcgtgcac caccggcttc acgcgcccgc
    10081 tcagcgacat cgcccacagg gacaccagca tgaggatgcc gaaggccacg tggaagccgt
    10141 ggaagctggt cagcacgaag aacgccgagc tgaacgggct ggtgtccagc ttcatgccct
    10201 cggccacgaa caccgtgaac tcgtagaact ggccggccag gaacgtcgag cccagcaggg
    10261 cggtgcccag ggtccacgtg cggaacttgc gcatgtcgcc ttcctggatg gccgccagcg
    10321 ccagcaccat cgacagcgac gacatcagca ggatgaacgt cgacaccgac gtgaacggga
    10381 tgtcgaagat ctccggcccg gggccgccgt tggtgcggtc caggtacatc aggtacgtgc
    10441 tgatgagcgc gccgaagaac aggcactcgg agccgatcag cgcccacatg cccatcttgt
    10501 tgttggacag ccccatggag gtgtggtgct cgccggtgcc ccccgcatcc gaatgggcct
    10561 cggtggccgc ggtcacgtcc gccatcagtg cgtctcctcc gcgctcggct cgagcaccca
    10621 gccggcgatg ccgccgaaga tcaccagacc gccgagcgcg atcaggatgg tgctgtcgaa
    10681 gatcacgccc cagccgccga tcggcatgcc ggcggccacg atcagcgggt accaggacgg
    10741 gtccggcatg tggatcgcgg cgtgcccgcc ggactcgccc actggttcgt ggtgcgcggg
    10801 gtcgggctcg gccgcctcgg ccggcacgtc ggccgcgtgg tcttgccccg gctcatgccg
    10861 gtggtcttgg ctggcgccgg cgggcacctt ctccggctcg ccctcgtgcg tgctgccgta
    10921 cttgcggaac caggcctcgt cgcgctcttg cacgatgggg atctcgtcga agttgtgcgc
    10981 cggcggcggc gagctggtca gccactccag cgtgcgcgcg tcccaggggt ccgcgccggc
    11041 cgtcttcggc ccgcgcaggc tggtcgccag gttgtacagg tacaccagca cgccgaccgc
    11101 cagcagcagc gcgccggcgg tctcgatgcg gttcatcagc tcccagccca tgccctccgg
    11161 gtaggtcgcc gtgcgccgcg gcatgccgtt caggcccacg atgtgcatgg ggccgaacgt
    11221 gaggttcatg cccacgaaga agatccagaa gtgcagcttg ccgagcttgt cgttcatgaa
    11281 cttcccggtg accttcggga accagtagta ggccccggag aagaagccca tgagcgcgcc
    11341 gccgaacagc acgtagtgga agtgcgccac cacgtagtag gtgtcggtct gctggtagtc
    11401 gtggggcacg acgccgtgcg tgacgccgga caggccgccg atggtgaaca gcgacaccag
    11461 gccgatggcg aacagcatgg gggtggggaa gcgcagcttg ccgccccaca tcgtgcccat
    11521 ccagttgaag atcttgatgc cggtgggcac cgcgatgaac atcgtggtga ggccgaacgc
    11581 ggcgttggcc accgggccca ggcccaccgc gaacatgtgg tgcgcccaca cgccccagcc
    11641 catgaagccg atcgcgatgc cggagaacac cacggccgtg tagccgaaca gcggcttgcg
    11701 gctgaacgtc ggcagcacct cgctgacgat gccgaaggcc ggcaggatca ggatgtagac
    11761 ctccggatgg ccgaacagcc agaacatgtg ctgccacagg atcgggtcgc cgcccgcgga
    11821 gggccggaag aagttggccc cgaaggcgcc gtcgaacatg agctgccaca gcgccacgcc
    11881 gatgatcggc agcgcgaaca gcagcaggaa cgcgaccacc agcgtcatcc aggtgaacac
    11941 cggcatgcgc agcagcgtca tgcccggggc gcgcatgttg aaggtggtca cgatgaagtt
    12001 gatcgccgat gccagcgacg cgatgccggc gatctgcaag ccgatggagt agaagatcat
    12061 gcggctcatc tcggcgccgg ccgtgccgac ggtctggccg atcgggtcac cgcccagcag
    12121 cgacagcggc gcgtaaccga accagccgcc gtcgggcatg ccgcccagga acaggctgga
    12181 gtaggtgaag atgcccgcgg ccaggaacac ccagtagctg aacccgttca ggcgcgggaa
    12241 cgcgacgtcg cgcgcgccga tctgcagggg cagcaggtag ttcatgaacg ccgagcccag
    12301 cggcatgacc acgaagaaga tcatggtgag cccgtgcatg gtgaacagct ggttgtactg
    12361 gtcgtaggtc agcacggagc tgttcggccc ggcgaggtgg atgcggatca gcagcgcttc
    12421 gaggccgccg accaggaaga agaacatcgc cgtgaagaag tacaggatgc cgatcttctt
    12481 gtggtcgacg gtggtgaacc agctggccgg gccggtggtg gcccgggggc gccggaacag
    12541 gctgccgccg gttgagggct tctcggcgat cgcggtcatt cgagcttctc cctgacaggg
    12601 ttgggggttg gcggcgtcgg cgttacttca ggctctgcag gtaggcgacc agcgcctcga
    12661 tctgctcttc cgacaggccc aggttgggca tctgcgcgcc ctgcttcacc gactgcgggt
    12721 cgcgcagcca ggcggcgagc tgttcggcgt cgctgttgtc gaacaggccg ccggcgaaga
    12781 tggagcggct ggcgaagtgg gtcaggttcg ggcccacccg cacgccggcc gcgccgccgc
    12841 cggaggtctc gtagccgttg atcgcgtggc aggcgatgca gccggccgtc tcgaacagct
    12901 ccttgccctg cgccgccagg ccgctctggc cgtcgaaggc gggctcggcc tgcgcggtca
    12961 gccactcctc gaagtcgccc gggtcgtggg cgaccacctc gaagcgcatc acggcgtggc
    13021 cgatgccgca gaactccgcg cactggccct cgtagcggcc ggggttgtcc gcctcgatgt
    13081 acatcgtgcg gacgtggccc ggcaccacgt cctgcttgcc ggccagcttc ggcacccaga
    13141 aggagtggat cacgccgtcc agcgactgcg ggttgcgctc gtcggactcc tcaccgggga
    13201 ccacggagtc cgggatctgc ggcgacagcg cctccatgga caggaacacg gggcgcccgg
    13261 tggggatgtg cagctcgttg gcggtgacga ccccctggcc ctcctcgccg gtgtactcga
    13321 actcccacca gaactgcttg ccgatcacgc gcacctgcag cgcgtcgtcg ggctcgtcgg
    13381 ccagcgcgaa gatcgtgcgc gtggtcggca ccgcgatgat cgccaggatc agcgccggga
    13441 tgatcgtcca cagcagctcc agcttggtgt tgccgtggag ctggtggggg atcgccgtgt
    13501 cgtcgccccg gctgcggaag cgccacatca ccacgagcag gccaagctgc acgaacacga
    13561 acaccccgat ggcgatgggg tagacgatgt cccagagctg gtcctgctgg cgcgcgaccg
    13621 gcccctcggg ggacagcgcg tcctgcggga gatcggcgtt ggcgcagccg gccagcagca
    13681 gcatggcgac ggcgagcagc gcccatccga tcagaccgga ccggcggggg cgaccagccc
    13741 tcatgggttc ctccagctcg aagtgacggc gttgtgattc acccgtgcgc acgccgcggg
    13801 cggccatagt ttgtcagacg aggtgcgccc ttgcccaaac ggcgcaccgc gcgggttcca
    13861 cgcgggcccg cggcatgcgc caagatcgca cggcgcgggc cagacgcgac gtcaggatcg
    13921 acaggatgcg gggcacggcc gaaaccgacg tggagaccgg gctgcggcgc gtcgtcgtgg
    13981 gcttccgggc cgccgccgtg ctgtggatgg cgctgctgct gctggtcggc gggctgccgc
    14041 ccgggctgcg tccggcgctc gccgccgcag gcgtgctggg gctcgcgggc gcgtgggcgt
    14101 tcctcaccgc gctgctcgcc gcggcccggc cggacgcgct gcgctcctgg gcttggctgg
    14161 ccgtggacac gctggtcgcc gccgtggtgg tgctcggcgc ggtgatcgtg gccgccgcgg
    14221 gctccttcgc cgtggccgtg gccgtgcccg tggccgcgtt cgcgggcggc tacccgttct
    14281 cggcggtgct gctgggcggc tgggcgcgcg gcgtgccggg cgggctcgcg gccgcgtcgg
    14341 tgctgtcggc cgccaccgtg gcccgggacg ccgcgggcgc gcggggctgg accctggagg
    14401 actcgctgcc cgcgacgctc gtctacctgg cgggcgcgct ggtgaccgcc tggggcgtgc
    14461 gcgtgctgcg gcgccaggac gccgagcggc gcaccgcgct gctggcgctg gcggaggagc
    14521 gcgccgccac ggcgcgcgag cgcgagcgtg cggagacggc cgcgcacctg cacgactcgg
    14581 tgctgcagac gctggcgctg gtgcagcggc gcgccgagga cccggccgcc gtgcgcgcgc
    14641 tcgcccgcga gcaggagcgc gagctgcgcg cctgactgtc cggcgggccg gcgagcggag
    14701 gcagcgcggc ggcggccctg gaggagcgct gcgcagaggt cgagcgcgac cacggcgtgc
    14761 gggtggacgt ggtggtcagc ggcgaccgcg cgctggacga gccgctggcc gcgctggtgt
    14821 ccgccgcggc cgaggccgtg cgcaacgcgg cccgccactc cggcgcgccg gaggtcgcgg
    14881 tgtacgtcga gcttggcgcg cagccggtgg tgttcgtgcg cgaccgcggc cgcggcttcg
    14941 acccggacgc ggtgccgccc gaccggcgcg gcatcgccga gagcgtgcgc ggccggctgc
    15001 gccgccacgg cgggcaggcg ctgctgcgca ccgcgccggg gcagggcacc gagtgggaac
    15061 tgcggctggg ggcgtcgtga cgcgcgagcc ggccaccccg gaggacttgc ggcgcgccgc
    15121 cgccgccgtg gtcgcagcgc tgtggccgca ccgcgatggc gactggcagg tgcgcgcggg
    15181 cgagctggac tggacctgcc tggacacgct gggccacacc gtggacgcgc tgcgcttcta
    15241 cgcggccaac ctggccacgc gcacgcgcgt gcggcgggcc tacccgtggg cggaggcctt
    15301 cggcggcacg ccggaggtgt ggctgctggc gctggaggcg accgcggagc tgctggcggt
    15361 ggtcgccgag accacggcgg atgacgaccg ggcgttccac ccggcgggcc tggccgacgc
    15421 cgagggcttc ctggccatgg gctgcgacga ggtgctcgtc cacggcgccg acgtcgcgca
    15481 agggctcggc gtcgcgctgg aggcgcccga cgacgtggcc gcgctcgtgc tcgcgcggct
    15541 gttcccgtgg gtggagccgg gcgacggcga gcgggcctgg gacctgctgc gctgggccaa
    15601 cgggcgcacc gagctgccgg ggcgtgcgag gctggggcca gactggtggt ggcactgcgc
    15661 gccgttggac gagtgggacg gctccgggcc ccgccggcgc agcgagcccc cgccgcgata
    15721 ggagcagccg tggcgctgtc cgtgttcctg gtcgacgacc accgcctgtt ccgctccggc
    15781 atccgggcgg agctggccgg gcgcctgcac atcgtgggcg aggcgggcac ggtcgacgag
    15841 gccgtggagg gcatcgtgcg gctggatccg acggtggtcc tgctggacgt gcacctgccg
    15901 gatggcggcg gcccggcggt gctggcgcgc tgccgcgcgg ccggctacgg cgggcagttc
    15961 ctggcgctgt cggcctccga cgacccgaag gacgtcgtgg aggtgatccg ggccggtgcc
    16021 cgcggctacg tcaccaagac ggtggacgcc gcggagctgg tggacgcggt gcgccgcgtg
    16081 gccggcggcg acgcggtgtt ctcgccgaag ctcgccggct tcgtgctgga cgcgttctcg
    16141 tccatccccg cgggacaggt cgacgccgac ctggcgcgcc tgtccccgcg ggaacgggag
    16201 gtcctgcagc tggtcgcccg cggctacacc taccgcgaga tcgccgagcg cctggtcctg
    16261 tcggtcaaga ccatcgagag ccacgtctcg gcggcgctgc gcaagctcca gctgtccaac
    16321 cgccgcgacc tcgcccgctg ggcgggcgag cgcggcatcg actaggcggc gcggcggcgg
    16381 cgctggccgc cgggacggcc gctgccctgg ccctggccct ggccctgacc cttggcgcgg
    16441 ggctgaccct ggcgctggct cgcgccctgg cgctggccct gccggggatt gcggcccggc
    16501 gccgccgcgc gcggccgctc ggcgcgctcg gtgcggtcga tcggcacggg cgccggcatc
    16561 ggcacgccgt cggcgagctg gtccagcgcc ggtgcctgga tcggctggcg catgcccagc
    16621 gcgcgctgca gcgactgcac gtccttgcgc ttctccgggg agaccagcgt gacgacgatg
    16681 ccgtccgcgc cggcccggcc ggtgcgcccc gagcggtgca cgtagtcctt gtccgtgccc
    16741 gccgggtcga agtgcaccac cacgccgacc ttgtcgacgt ggatgccgcg cgcggcgacg
    16801 tccgtggcga ccaggacgga ggccttgccc gcggtgaagt cggccagggc gcgctggcgc
    16861 tgcccctggc tgcggtcacc gtggatcgcc accgcgcgca cgccctcgcg cacgagctgc
    16921 ttggcgacgc ggtcggcgcc gtgcttggtg cgcgtgaaca cgatcgcggg gctggagcgc
    16981 cccacgatgt cgcgggtcac ccgcacgcgg tcgctggagc cggccgtcca gaacaggtgc
    17041 cgcacgtcgc cgtcgtcctc ggcgctggcc gcgagctcgt ggcgcgcggg gtcgcgctgg
    17101 tagcggcgga tcaccacgtc gacgtcgccg tccaacgtgg cggagaacag cagggtctgg
    17161 cgctccggcg aggtctggtc cagcaggcgc ttgaccgcgg gcaaaaagcc catgtcggcc
    17221 atgcggtccg cctcgtccag caccaccagg cgcacgttgg acaggttgac gacgttgcgg
    17281 tcgatcaggt cctccaggcg gccggggcag gcgacggcga tgtcgacgcc gcgctccagg
    17341 cgctggcggt ccttgccgat ggaggtgccg ccgtagaagg aggcgacgtt caggcctcgc
    17401 gcctgcgcca gcggcgcgat ggtctcggcc acctgggcgg ccagctcgcg cgtcggcacc
    17461 aggatgaggg cgctgggctt gcgcgggccg cccttgcggg ccagcgaggc gaccggcacg
    17521 ccgaaggcga gggtcttgcc gctgcccgtg ggcgcgcggc cgcacaggtc acggcccgtc
    17581 atcgcgtcgg cgatggtcag ggcctggatg gggaagggtg cgtcgatgcc gcgctttgcc
    17641 agcgcggcga tgatcttcgg cgacacgccg agatcgctga aagtgctcat ggaaatctca
    17701 ctgccgttcc gcgcgcgcca atggcgtgcg ctggtcgtgc gcgcacggac gtcccgtacg
    17761 cgggcgtggg gccgccctgc cgtgaaggcg ggggccgcac ttggttgaac acgcccgtac
    17821 gagagaagcg attcctccgg gaactgcttg gaggaagccc gctgatcgag tccggcgccg
    17881 agagatattg accgcttggc gcgcgtgttc cgcgaggtcc gtacaggacc cgcgacgacg
    17941 actgtacccc atgggggacc ctgaggcagg ttccgggtcc ttccctgatg gccgcccggc
    18001 tcgcggcggc gaccatcacg gcatgaacga actgctgcgt cagccgctgt cccgccggat
    18061 cctggtcacc gccgccatcg gctcgctggc cgactggacg ctgttcgccg cgctggtcgc
    18121 cgtcgtggcc ggcctcaccg gcggctcggt gttcgccgtc gcgctggtca cgggggcgcg
    18181 catcctgccg tccgtcctgc tcggccccct ggtgg
//
LOCUS       DATMKC010000011         2873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_20234, whole genome shotgun sequence.
ACCESSION   DATMKC010000011 DATMKC010000000
VERSION     DATMKC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20234"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VNU01_00390"
     CDS             complement(<1..914)
                     /locus_tag="VNU01_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH31106.1"
                     /translation="MSRARAVLAIAALALRRLARDRLGLLFTVALPFTVILLVGLAFG
                     DEGGEVRLGVAFDGAPSAEAERLAAALEARDGLLVVRLPDAATLRADVREGDVDAGLL
                     IPPGTGTRPVLVGPADGGGMAAARPLVGAVLARLADPAAEPAPVRVERFGEDAPLGDL
                     PRFGYTAPANLVLFTFITSLGAASGLIESRVIGTRRRLRAAPLSAGHVLAGEAFGRLL
                     VAVAQALVVVLGAALIFGVAWGDPAGVTAVVGAFALVATAAGLLLGTVLRTPQQASAI
                     GPMAGIALGMLGGCMWPLEIVPPAMRAAG"
     gene            complement(911..2014)
                     /locus_tag="VNU01_00395"
     CDS             complement(911..2014)
                     /locus_tag="VNU01_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH31107.1"
                     /translation="MRASLRISGLVALRDLRGRLRDRSALVTAFLAPVALAAILSLAF
                     GAGELPEIRVAVAPVAGTTQDAEAAALLRALASVPAGQSVLRVQSMDGEAQARAALDD
                     EAADAAVLVRPGPPRAELLVLRRADAPVSGEVAEALAHNAALAVAVGARGGEAMPLAE
                     ERLKGRSPRSADYYGPAMAVFFVFFVMAFAPLSLIAERRDGTLARLAASPVRMGAVLA
                     GKGLAVFVLALVSVLVVWAVTRTLFGAHWGSPPAVLLLTVGVVLAAGGLTTLAAAGAR
                     TERQADGQVSAVVFLGALLGGNFLPLDALPGFLRAASLATPNGWAMRGFTELANGGEL
                     AGVLPNVAVLLAMALGAGGVALLRAGRGAGMRP"
     gene            complement(2014..2769)
                     /locus_tag="VNU01_00400"
     CDS             complement(2014..2769)
                     /locus_tag="VNU01_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH31108.1"
                     /translation="MITVLRCEGLRQAYGRRVAVDGVGFTIAEGEAYGLLGPNGAGKT
                     TTISMLCGLLSPDAGHVEVAGHRMDAEPFAAKALIGYVPQELALYPDLSARENLTFFG
                     RVQGLSGAELRARVAHALEVAGLVDRAGDRVEAYSGGMKRRVNIAVGLLHRPKLLVLD
                     EPTVGVDPQSRAAILDTVQALNADGMALLYTSHYMEEVERVCHRIGILDEGRLIAEGT
                     HDELVGLVGAQPGAPRPTLESVFLHLTGKALRD"
BASE COUNT          401 a         1144 c         1058 g          270 t
ORIGIN      
        1 cccgccgccc gcatcgccgg cgggacgatc tccagcggcc acatgcagcc gcccagcatg
       61 cccagcgcga tgcccgccat cggcccgatc gccgaggcct gctgcggcgt gcgcagcacc
      121 gtgccgagca gcaggcctgc ggccgtcgcg accagcgcga acgcgcccac cacggccgtc
      181 acccccgcgg ggtcgcccca ggccacgccg aagatgagcg cggcgccgag caccacgacc
      241 agcgcctgcg ccaccgcgac gagcaggcgg ccgaacgcct cgccggccag gacgtggccg
      301 gccgacagcg gcgccgcgcg cagccgccgc cgcgtgccga tgacgcgcga ctcgatcagc
      361 ccgctcgctg cacccagcga cgtgatgaac gtgaacagca ccaggttcgc gggcgcggtg
      421 tagccgaagc gcggcaggtc gcccaggggc gcgtcctcac cgaaccgctc cacgcgcacc
      481 ggcgcgggct cggccgcggg gtcggccagc cgcgccagga cggcgccgac cagcgggcgg
      541 gcggccgcca tgccgccgcc atcggcgggg ccgaccagga cgggccgcgt gccggtgccc
      601 ggcgggatca gcaggcccgc gtcgacatcg ccctcgcgga catccgcgcg cagcgtcgcg
      661 gcgtcgggca ggcgcaccac gagcaggccg tcgcgggcct cgagcgccgc cgcgaggcgc
      721 tccgcctcgg cggagggcgc gccgtcgaac gccacgccga ggcgtacctc gccgccctcg
      781 tcgccgaagg ccaggccgac cagcaggatc accgtgaacg gcagcgccac ggtgaacagc
      841 aggcccaggc ggtcgcgggc gagccggcgc agcgccagcg cggcgatcgc caggacggcg
      901 cgggcgcggc tcacggccgc atccccgcgc cgcggccggc gcgcagcagc gcgacgccgc
      961 ccgcgcccag cgccatggcc agcagcaccg cgacgttggg cagcacgccg gcgagctcgc
     1021 cgccgttggc cagctcggtg aagccgcgca tcgcccagcc gttcggggtg gccagcgacg
     1081 cggcgcgcag gaagcccggc agcgcgtcca gcggcaggaa gttgccgccg agcagcgcgc
     1141 ccaggaacac caccgcggac acctggccgt cggcctggcg ctccgtgcgc gccccggcgg
     1201 cggccagcgt ggtgagcccg cccgccgcca gcaccacgcc caccgtgagc agcagcaccg
     1261 ccggcgggct gccccagtgc gcgccgaaca gggtccgggt caccgcccac accaccagca
     1321 cgctgaccag cgccaggacg aacacggcca ggcccttgcc ggccaggacc gcgcccatgc
     1381 gcacggggct ggccgccagg cgcgcgagcg tgccgtcgcg gcgctccgcg atgagcgaca
     1441 gcggcgcgaa ggccatcacg aagaacacga agaacaccgc catcgccggg ccgtagtagt
     1501 cggcgctgcg cgggctgcgg cccttgaggc gctcctccgc cagcggcatc gcctcgccgc
     1561 cccgcgcgcc gaccgccacg gcaagcgcgg cgttgtgggc gagcgcctcg gcgacctcgc
     1621 cggacacggg cgcgtcggcc cggcgcagca ccagcagctc ggctcgcggg gggccagggc
     1681 gcaccagcac ggcggcgtcc gccgcctcgt catccagggc cgcgcgcgcc tgcgcctcgc
     1741 cgtccatcga ctgcacgcgc aggaccgact ggccggcggg cacgctggcc agcgcgcgca
     1801 gcagcgccgc cgcctcggcg tcctgggtcg tcccggcgac cggcgccacc gccacacgga
     1861 tctcgggcag ctcccccgcg ccgaacgcca gcgacaggat cgccgccagg gcgaccggcg
     1921 cgaggaacgc cgtgaccagg gcggagcggt cgcgcagccg gccgcgcagg tcccgcagcg
     1981 ccaccagccc gctgatgcgc agggaggcgc gcatcagtcg cgcagcgctt tgccggtcag
     2041 gtgcaggaac accgactcca aggtgggccg cggcgcccct ggctgcgcgc ccaccagccc
     2101 gaccagctcg tcgtgcgtgc cctcggcgat gaggcggccc tcgtcgagga tgccgatgcg
     2161 gtggcagacg cgctcgacct cctccatgta gtggctggtg tacagcagcg ccatgccgtc
     2221 ggcgttgagc gcctggacgg tgtccaggat ggcggcgcgg ctctgcgggt ccacgcccac
     2281 ggtgggctcg tccaggacca ggagcttggg ccggtgcagc aggcccacgg cgatgttgac
     2341 ccggcgcttc atgccgcccg agtaggcctc gacccggtcg cccgcgcggt cgaccaggcc
     2401 cgcgacctcc agggcgtgcg cgacgcgggc gcgcagctcg gccccggaca ggccctgcac
     2461 ccggccgaag aaggtcaggt tctcgcgggc gctgaggtcg gggtacaggg ccagctcctg
     2521 cgggacgtag ccgatcagcg ccttggcggc gaacggctcg gcgtccatgc ggtggccggc
     2581 gacctcgacg tgccccgcgt ctggcgacag caggccgcac agcatcgaga tggtggtggt
     2641 cttgccggcg ccgttggggc ccagcaggcc gtacgcctcg ccctcggcga tggtgaagcc
     2701 gaccccgtcg acggcgaccc gccggccgta ggcctggcgc agcccctcgc accgtaacac
     2761 tgttatcatt cgctgcacgg tacaccacat tcccccgagt tggaagatca ggcccacata
     2821 cggaccacat cttccaactc ggaggaattg gcccggcggg gcgggggcgg ggg
//
LOCUS       DATMKC010000012         3858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_189047, whole genome shotgun sequence.
ACCESSION   DATMKC010000012 DATMKC010000000
VERSION     DATMKC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189047"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3043)
                     /locus_tag="VNU01_00405"
     CDS             complement(<1..3043)
                     /locus_tag="VNU01_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31109.1"
                     /translation="MSTARQAGRVAARGRVAGALLATAVALSALTGGAPLAASELPGD
                     TAEDAADVRASSLVVQRPRGSGTNPGRATSTHFWVSSSARRGYFLANERHQGSLRGFE
                     RLVAVDLATGARTGHAELSPRAYGNPPGNLYTAQGADNAVAIDEEGGRLFVATQAPTV
                     EVNGEQLQAAGPSRAAAASRGELCARPGGTTPLYGCVNGVTVLDARSLDELGHMTLRG
                     LNAEAPLAAPQLLAMSYVPPRGDLGAKLLMLVEDTAHAAEFDAQPRASTRAQSAAVVV
                     LVQFDVASATQEWTLRLDECRGAMASNRSKPYDALKSHRYPAAVLAGGSPERPFVSVA
                     CHMVAQSLQGAVLRVPLTPSGAPAPLPVGPGQAGAVPGLPAAAAGETAPAVTGDHPGR
                     RILVPGPDKVHEMIADPASGRIAMQVLDGEPAAQVWWIFDTGLMRFVGTVGIGGPVAP
                     GASMSAVDGGRLYVLAKPETAGGRTFSGGLFAADIARTPVSQALVYPRMAERFPADYG
                     NSAESARWALTVQRLDGGARRLWFPSSWNIDHLQTYAAIEDHRPDPRLPAPEDYAGRT
                     LDLDEAEGVTDASLDGAARGYGVRVLLVGGAESLSRAGPADPVGSVEQCNDDDWGEEN
                     NAIANTIGPVIGGETSGGPCHVAGVRLTSPCLHGDREIVLAAVGPDGPAVVDGAGARG
                     AANPITVDSLTGADLEAPVSRCAGQDWSQLWTAALFGRPPAGEPTLSTDLGELLAECV
                     SGGQRTSGQSGDPVVNGYSAAVSCQEDEASGHAYARGTTVEGASVAEALSSFRIYRDP
                     GRGIVARVESVARGVALDGVLRIDTVRGTAESWANGRRQPVERAQRPEGYDPDCDLER
                     TAGTCFTRHVFGVRSPAYSCGPCGDEHALLEGLNAGLGPMGLLFQLREPDARLRQGAE
                     NGFTAAIQKPEADRFGDLVLNNDLLQTVLPTLEVIRNAAPNRVLSRKGSKGRQIYQFA
                     GVEVSSSYGIACLHVYDAETGVCAPQAEPAGAIRVSLQDTGG"
     gene            3321..>3858
                     /locus_tag="VNU01_00410"
     CDS             3321..>3858
                     /locus_tag="VNU01_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31110.1"
                     /translation="MDFGLTPEQEAIRATARKFCDAEVAPGARDLDRAEAFPEELLPK
                     LFEVGFLAAPIPEAYGGMGLDYLSYGLIVEELGRTDGSIRSMVSVNVGLVGMTILKFG
                     TEEQKAEWLPRLASEGLGAFGLTEPGAGSDPSSMTTGASRDGGDWILNGSKMFITNGS
                     RGIVTIVFARDGETGRPTA"
BASE COUNT          474 a         1446 c         1445 g          493 t
ORIGIN      
        1 ccccgccggt gtcctgcagc gacacgcgga tcgcgccggc cggctccgcc tgtggcgcgc
       61 agacgccggt ctcggcgtcg tagacgtgca ggcaggcgat gccgtaggag gacgacacct
      121 ccacgccggc gaactggtag atctgccgcc ccttggagcc cttgcggctc agcacccggt
      181 tcggcgcggc gttgcggatc acctccagcg tgggcagcac cgtctggagc aggtcgttgt
      241 tcagcaccag gtcgccgaag cggtccgcct ccggcttctg gatcgcggcc gtgaagccgt
      301 tctccgcgcc ctgccgcagg cgggcgtccg gctcgcgcag ctggaacagc aggcccatcg
      361 ggccgaggcc cgcgttcagg ccctcgagga gggcgtgctc gtcgccgcac ggcccgcagg
      421 agtacgccgg ggagcgcacc ccgaacacgt ggcgggtgaa acaggtcccg gccgtgcgct
      481 cgaggtcgca gtcggggtcg tagccctcgg ggcgctgcgc gcgctccacg ggctggcgcc
      541 ggccgttcgc ccacgactcc gccgtgccgc gcaccgtgtc gatgcggagc acgccgtcca
      601 gcgcgacgcc ccgggccacc gactccaccc gcgcgacgat gccgcggccc gggtcgcggt
      661 agatgcggaa gctggacagc gcctcggcga cgctggcccc ctccacggtc gtgccccgcg
      721 catacgcgtg gccggaggcc tcgtcctcct ggcaggacac cgcggcgctg tagccgttga
      781 ccaccgggtc gccggactgc ccgctggtgc gctggccgcc ggagacgcat tcggccagca
      841 gctcgccgag gtcggtgctg agcgtgggct cgcccgcggg cggcctgccg aacagcgccg
      901 cggtccacag ctggctccag tcctggccgg cgcagcgcga caccggcgcc tccaggtccg
      961 cccccgtcag cgagtcgacg gtgatcgggt tggccgcgcc ccgggcgccg gcgccgtcca
     1021 ccacggcggg gccgtccggg cccacagccg cgagcacgat ctcccggtcg ccgtgcaggc
     1081 acgggctggt gagccgcacg ccggcgacgt gacagggacc gccggaggtc tcgccgccga
     1141 tgaccgggcc gatcgtgttg gcgatcgcgt tgttctcctc accccagtcg tcgtcgttgc
     1201 actgctcgac actgccgacg gggtccgcgg ggccggcgcg cgacagcgac tcggcgccgc
     1261 ccaccagcag gacgcgcacg ccgtaccccc gtgccgctcc gtccagcgac gcgtcggtga
     1321 cgccctcggc ctcgtcgagg tcgagcgtcc tgcccgcgta gtcctccggc gccgggaggc
     1381 gggggtcggg gcggtggtcc tcgatggccg cgtaggtctg gaggtggtcg atgttccagc
     1441 tggaggggaa ccacagccgg cgggccccgc cgtccaggcg ctgcacggtg agcgcccacc
     1501 gggcgctctc ggcgctgttg ccgtagtcgg ccggaaagcg ctccgccatc cgcgggtaca
     1561 ccagcgcctg ggacaccggg gtgcgcgcga tgtcggcggc gaacagcccg ccggagaacg
     1621 tgcggccacc cgcggtctcc ggcttggcca gcacgtacag ccgcccgccg tccaccgccg
     1681 acatcgaggc gcccggcgcc acgggtccgc cgatgcccac ggtgccgacg aagcgcatga
     1741 gccccgtgtc gaagatccac cacacctgcg cggccggttc gccgtcgagc acctgcatgg
     1801 cgatgcggcc cgaggccggg tccgcgatca tctcgtgcac cttgtccggg ccgggcacca
     1861 ggatgcgccg tcccgggtgg tcgccggtca ccgccggggc cgtctcgccc gccgccgcag
     1921 cgggcaggcc cggcacggcg ccggcctggc cggggccgac cggcaggggg gctggcgcgc
     1981 ccgacggcgt cagcggcacg cgcagcacgg cgccctgcag gctctgggcc accatgtggc
     2041 aggccacgga gacgaacggg cgctccgggg agccgccggc gagcaccgcc gcggggtagc
     2101 ggtggctctt gagcgcgtcg tacggcttgc tgcggttgga cgccatggcg ccgcggcact
     2161 cgtccaggcg cagcgtccac tcctgcgtgg ccgacgccac gtcgaactgc accagcacca
     2221 ccacggccgc cgactgcgcg cgcgtgctgg ccctgggctg cgcgtcgaac tcggccgcgt
     2281 gcgcggtgtc ctccacgagc atgagcagct tcgcgcccag gtcgccgcgc gggggcacgt
     2341 aggacatcgc cagcagttgc ggggcggcca gcggagcctc cgcgttgagc ccgcgcagcg
     2401 tcatgtgccc gagctcgtcc agcgagcggg cgtccagcac ggtcacgccg ttgacgcagc
     2461 cgtacagcgg cgtcgtgccg cccgggcggg cacagagctc gccgcgcgag gcggccgccg
     2521 cccgggaggg cccggcggcc tggagctgct cgccgttgac ctccaccgtg ggcgcctgcg
     2581 tggcgacgaa gagacgcccg ccctcctcgt cgatggcgac cgcgttgtcc gcgccctggg
     2641 cggtgtacag gttgccgggc gggttgccgt acgcccgggg cgacagctcg gcgtggccgg
     2701 tccgcgcacc ggtcgccagg tccaccgcga cgaggcgctc gaacccgcgc aggctgccct
     2761 ggtggcgctc gttggccagg aagtagccgc gccgggcact gctcgagacc cagaagtggg
     2821 tcgaggtcgc gcgcccgggg ttggtgccgg agcccctggg ccgctggacc accagggagg
     2881 acgcgcggac gtcggcggcg tcctcggccg tgtcgccggg cagttccgag gcggccagcg
     2941 gcgcgccgcc cgtcagtgcc gacagcgcca cggcggtggc gagcagcgcg cccgcgacgc
     3001 ggccgcgagc ggccacacgg ccggcctgtc gggctgtgct cacgctgggc tcccacctcg
     3061 tcggacgatc gtcgcggcgt tgccgtgccc gcccggcggc cgcgcctcag aaagattcta
     3121 cgaagcggat ggatcctcct tacgcgacca tgccacttat cggtggcgtc gttgggggtg
     3181 tgtccggcta agtccggaca gacgtggcgg agcgccggag aaaaactttg cggcggcgtc
     3241 ccccggcacg gcgtgcggcc cagcgcctag catgtgcccg cccgggccag cggggcccgt
     3301 ccgcttcatg gagcgcacgc atggacttcg gtctcacccc agagcaggag gcgatccgcg
     3361 ccaccgcgcg caagttctgc gacgccgagg tggcgcccgg ggcgcgcgac ctcgaccgcg
     3421 ccgaggcctt cccggaggag ttgctgccca agctgttcga ggtcggcttc ctggccgcgc
     3481 cgatcccgga ggcctacggc ggcatgggcc tggactacct gtcctacggg ctgatcgtgg
     3541 aggagctcgg ccgcaccgac ggctccatcc ggtcgatggt gtcggtgaac gtcgggctgg
     3601 tcggcatgac catcctgaag ttcggcaccg aggagcagaa ggccgagtgg ctgccgcggc
     3661 tggcctcgga aggcctcggc gcgttcgggc tgaccgagcc gggggcgggc agcgacccct
     3721 cctcgatgac cacgggcgcc agccgcgacg gcggcgactg gatcctcaac ggctccaaga
     3781 tgttcatcac caacggcagc cgcggcatcg tcacgatcgt gttcgcgcgc gacggcgaga
     3841 ccggccggcc caccgcgt
//
LOCUS       DATMKC010000013        14021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_46600, whole genome shotgun sequence.
ACCESSION   DATMKC010000013 DATMKC010000000
VERSION     DATMKC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14021
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46600"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..415)
                     /locus_tag="VNU01_00415"
     CDS             complement(20..415)
                     /locus_tag="VNU01_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH31111.1"
                     /translation="MTAFPSIGHVALTVSDLPASVAWYGKLFGVEPVLDENVGAFHHT
                     VFPVGGGLIGLHAHPSTAGEDRFDEYRVGLDHVAFGCADRSELEEWAGRLDGLGVAHS
                     GIVDAGYGSGLSFRDPDGIALEFFAPPAG"
     gene            complement(412..1365)
                     /locus_tag="VNU01_00420"
     CDS             complement(412..1365)
                     /locus_tag="VNU01_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWH31112.1"
                     /translation="MTVVTVAAVQAAPVFLDREATVDKAVDLIGKAAADGAQLIAFPE
                     SFVPTYPDWVWRSRPWADGPSRWYERLFDQAVEIPGPATQRLAEAAQQAGAYVAMGVT
                     ERDAGTLYNTVVWLDPQGRVAHKHRKVMPTGGERLVWGMGDGSTLSVLDTPIGRLGAL
                     ICWENYMPLARYALYAQGIDIYVAPTWDNSDTWVPTLRHIAKEGRCHVVGVTPCLRGS
                     DVPADIEGRDGLYGGDEDWMSKGNSCVVGPEGELLAEPLVGAEGIVIAQVDVTAARLS
                     RRMFDPVGHYARPDIFRLEVDRRPKPAATFTDHDPQQGAHS"
     gene            1557..2069
                     /locus_tag="VNU01_00425"
     CDS             1557..2069
                     /locus_tag="VNU01_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31113.1"
                     /translation="MRDAVVETVVGVDRFPDDVRRLTTLDRVDYADLFCVPVDDARRR
                     TPEEWARALLEEGAIARRSARRLWTALGLRLGPPGSPDHVQGWRIAGRGDDWITVETG
                     SWYMSGQAVCKVDAGRVSIALFLRHDNPLAPAVWAPVSVFHRRAVPVMLAQAVAIVAG
                     RGGGAQEAAS"
     gene            2134..2640
                     /locus_tag="VNU01_00430"
     CDS             2134..2640
                     /locus_tag="VNU01_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWH31114.1"
                     /translation="MTETAPRTVGPEALARAAMANVDAHDFAGLRAAWHPDLELYFVA
                     TGKRVGQDGAAAEFREIFDAFPDFRIAPTRVLADGDTAIVEWEAHGTHTGAAYQGIAR
                     TGRRIELRGVDLMEFSDGLLRRNTVYYDGLSVARGLGLLPPDGAAQDRALKALFNVLT
                     RLRGLVRR"
     gene            2679..3833
                     /locus_tag="VNU01_00435"
     CDS             2679..3833
                     /locus_tag="VNU01_00435"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH31115.1"
                     /translation="MAGVATYGARGSLGLFVTPWEARFGASRADIALVSTVGFLVFAA
                     GQTVAGRIQESVPGRRLVLVGVALCALGYGGAAFAPSLPAVVLLVGVLAASGIALSAL
                     PVLSVLAADLVRSREGMVFGLLTAAGAGGQVVVLPLAAAALNGSERGALLVIAGVLGV
                     ALAGVALAVPRGAGVRPEDAAPSVPVRGFARLWREPRFWLLLVPFFVCGYTTTGLMDT
                     HFIPYAVDHHVPETVASGALATLAAFNVLGVLVAGGLTDRVDRGRLLAILYAGRAGSL
                     LLLPLLTSPRGLFVFAVLFGVVDFATVPPTTALTRRVFHTGGWAVALGVISAAHQVGS
                     ALGAYLGGWLYDRTGGYEIVFASAAVALLAAAAMSLRLAGEQDLAEARRT"
     gene            3839..6031
                     /locus_tag="VNU01_00440"
     CDS             3839..6031
                     /locus_tag="VNU01_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HWH31116.1"
                     /translation="MHRRDFLKAAGAGAVLAALPGVQLAARQPTGTLLAGAAVADITP
                     ELGGEFFGYVRPDIHAEGVALRLHAHALVLDDGVRKLALVSVDLGEPMVKAEVVERVR
                     HLGFDEETVLLAATHTHAGPNAATDWTAFQIAEAIRLADAARRPARAGWAVAHVPDAN
                     LSRSVEAHLANHGQDLLPGFGTPDLDPAGADHTRDTALRLLRVEGVDGRPIAAWTHFS
                     VHPTTFIPSNKLFSADFCRVANLRFVQRFAGSPAPVAMYTNGNQGDLITWFDDYNQHA
                     VADRIGARVARAMSAAWDDAGRALSSAVVVDARRTTVTYQGQEVEPGKRVGGPAGFFG
                     ASFFGGAKNGPSIFYWMGTEGKRRPAAAADPVHGRKILAAPAPWLPTVELQALRVGDR
                     LLLGIPGEPSVETGRRVRAAAMAGAPAGLADAAVVGLANGYHGYFTTPEEYDQQHYEG
                     GHTVFGKYTSLLVQHGHAGLAGELAARGRAPADSGAPAAPVAAPVGDGGTDGAIVAEP
                     TALTERMRTVELSWTGGPSGRDRPVGTPFVRVERRDAAGWAAVDDDLGHRMVWFQRGQ
                     TYTARWDIDPAMPTGTYRFRVTSARYELTSRPFQVGVSTGLRVRGVERDGPRLVFRAQ
                     NPPPDPERGLLSRPRTPAGGTLRFTQDGRERVATWDTAARGWVAAVAPGGGPVTVPAG
                     GLLDGLGNRNGHEAVLRAGEVAAVDWPAHIGPAGGQTPGPFGEGTFPP"
     gene            complement(6062..6643)
                     /locus_tag="VNU01_00445"
     CDS             complement(6062..6643)
                     /locus_tag="VNU01_00445"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HWH31117.1"
                     /translation="MRLFSFRPSLSVRGRKPKGLRGLAGKPLHPPLTDVPVGAYILAA
                     AFDVISFLGDGEPWARELYRAGTFVLAGGLLVAVLAAITGVADWVGSTSKGTQAWRTV
                     NAHAATMAALTVLVVVELVLRAGTYGAAAATPGIVLALTLVVAGLTALGGTIGGSLVY
                     DYGFNVETAGDSPVWHPSERDVLPADKGDGAAH"
     gene            complement(6674..7252)
                     /gene="folE"
                     /locus_tag="VNU01_00450"
     CDS             complement(6674..7252)
                     /gene="folE"
                     /locus_tag="VNU01_00450"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWH31118.1"
                     /translation="MQQRAPDAPAFDRVAAARAVRDLLVAVGADPDVEDLRDTPRRVA
                     AALDELLTPTPFTFTTFPNDGRYDELVLVRDIRFHSLCRHHLLPFSGVAHVGYVPGER
                     IVGLSKLARVVEFHARGLQVQETLTTSVADELERRLAPRGAGVVLEATHSCMSLRGVA
                     KPGARTVTSAVRGLLRDDHRTRAEFLSLVRAD"
     gene            complement(7329..8024)
                     /locus_tag="VNU01_00455"
     CDS             complement(7329..8024)
                     /locus_tag="VNU01_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH31119.1"
                     /translation="MTDARDDLEDQLDDIALLDEPTRRALYRYVVGRPDAVGRDEAAA
                     AVDVSRGLAAFHLDRLAEAGLLEVEYRRLSGRSGPGAGRPAKLYRRAERQVDVTLPPR
                     RFRLAAELLADALDRPEAGPARGALADAARERGRAEAARLAAGLGSRPSRARRLEALW
                     AVLSDCGYEPYQDGGAWRVRNCPFHPLASAHTELVCGMTGEFVAGAAGALALAPELAL
                     DPRPGECCVVIRF"
     gene            8142..9557
                     /locus_tag="VNU01_00460"
     CDS             8142..9557
                     /locus_tag="VNU01_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWH31120.1"
                     /translation="MEPGPLVIGAERVRTGQTIEVTSPYSGEVVGAVAKAGTEHLDAA
                     VAAARDAMSAPLPTHQRVAVLDAVAARLTERAEDFARLIAAEAAKPLRTARAEVARGI
                     DTLAFSAAAARTLTGEFLPLDASAAGEGKVGWVTRVPAGVVAAITPFNFPLNLVLHKL
                     GPSLAAGCAVVLKPASSTPLTAAALVELFHDCGLPPGWLNFTPCSGSTANHLVTHPGV
                     DVVSFTGSREVGWSIREQAPRKRVGLELGNNSPAIVEPDADWRAAARKIAAAGNSYAG
                     QSCISTQRVYVHDEIAAAFLDELAAAVGELVVGDPLDEATDVSALIDAGETERVRAWV
                     REAVAAGASVAAGGEQDGGVLRPTVLADVKQDMRVSREEVFGPVLAVQTYGDYDDALA
                     MANDTDYGLQAAVFTRDLGKALRAARVLRYGGVLVNETPTWRADLMPYGGVGDSGNTR
                     EGPAYAIRELLTEQRLVVLQP"
     gene            9587..10036
                     /locus_tag="VNU01_00465"
     CDS             9587..10036
                     /locus_tag="VNU01_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWH31121.1"
                     /translation="MKGKPYGTLALALAVHLLIMFALTYAGVYRLDHVYANLNRFYMA
                     LLMVAPMAIVMVAFMWHMFPRRALNVAILGLAVVLFAGTFAAIRAQALVGDMQFLRSM
                     IPHHSIAIKTCERAQISDPEITKLCEQIVRAQREEIAQMEDIIRRLD"
     gene            10097..11236
                     /locus_tag="VNU01_00470"
     CDS             10097..11236
                     /locus_tag="VNU01_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31122.1"
                     /translation="MRRAVTGGILASLLVLLGGAHAGLAQEPGYAPLDQPGPALRVPL
                     AELEASLTCDGALDGSRAAVLLIHGTNLDYETNFGWNYQRQFAALGWPHCGVDLPKKG
                     MDDIQVAAEHVVHAVRTVAARTGRPVQIVGFSQGGMIGRWALRFWPDTRDLVEEVVGL
                     APSNHGTLTAIPTCNPSCVPAYQQQRADSRFVAALNSRALTFGGVDYSVIYTRTDWVV
                     TPNHDGSASSLPAGPNAVNVAIQDICPGDSSEHLHLGSFDAVGYALVHDALTHDGPVD
                     PARIAPTVCAEPYMPGVDPATFAGDYAAYLRQVGEAYQETEAVTEEPPLACYVTASCA
                     EPAGQPPTGAEAPPAAPALPATGGGGLAGAALLAAAAASVRRLRR"
     gene            11294..12115
                     /locus_tag="VNU01_00475"
     CDS             11294..12115
                     /locus_tag="VNU01_00475"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWH31123.1"
                     /translation="MDTAVEPRGEGRFAAQLSQDWEIWGPNGGYLAGIALRAAAASSA
                     QPVPASLTCHHLSVASFDPVELEVVSLRATRRAESLRVTMWQQDKRIVESLVWLVTPG
                     DGLLHDADTPPDVADPDALPSLPELLGAERRNPPSYRFWDNFEARPLDWNGPFEQRPA
                     GEPRVRNWYRYTPTATFDDPVVDAIRSLILLDTMSWPAARRAHPPGPLPYVAPTLDVA
                     VQFHRAAPASEWLLVEGVAPVAEQGLAAYSAKVWSRDRRLLASAAGQMLCRPVPG"
     gene            12112..13125
                     /locus_tag="VNU01_00480"
     CDS             12112..13125
                     /locus_tag="VNU01_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31124.1"
                     /translation="MSARGMAAWVLAFALAACNPGFAPEPTPRPSVPDYEAEGMPTPA
                     PLDQAAVPVVALAGLPHHRVDVPGGPDWLAQDADRGGIFVKLDRGDVAELDPATGAVL
                     RTLGAPAPLCQGIGAGFGGVWTCGGAGVRRLEIASGEQTRDLPVRKAFEQGHLPAAFG
                     QVWVLTGTGEELVGLNPADGSVATTVALDARGHDLVFAPEALWVVSGPDDLVLGLYPR
                     QDVVAMRIGGIDNPRAVAVARDVWVAAPSGVYRIDRTTGALLGAIGLPLGQDTAIAAD
                     PNDVWVRSAAGFLHRIDARTGEVVERIDAGIAGGGDVLLTPDAVWVTAYDANVVVRLA
                     RQR"
     gene            13374..13889
                     /locus_tag="VNU01_00485"
     CDS             13374..13889
                     /locus_tag="VNU01_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWH31125.1"
                     /translation="MTMLSLTDAAQRFLSRRRIAVAGVSRTPQGHGANVVYQRLRQRG
                     YDVFPVNPNAEEVEGDRCYPDLRSIPGGVEAVVIGTAPAAAEGLVRECVDLGVTDVWM
                     HRSFGGGSVSSSAARYGREHGVMVIEGGCPLMFEPTGDGPHRFLKFVCTLAGTVPRTV
                     EEISPAAPASG"
BASE COUNT         1702 a         5270 c         5224 g         1825 t
ORIGIN      
        1 gttcttcgcg ccggcggcct cagccggccg gcggcgcgaa gaactccagg gcgatgccgt
       61 cggggtcgcg gaaggacagg ccggagccgt agccggcgtc gacgatgccg ctgtgggcca
      121 ccccgagccc gtccaggcgg ccggcccact cctccagctc ggaccggtcg gcgcagccga
      181 acgcgacgtg gtccaacccg acgcggtact cgtcgaagcg gtcctcgccc gccgtcgacg
      241 ggtgggcgtg cagtccgatc aggccgccgc ccaccgggaa gacggtgtgg tggaacgcgc
      301 cgacgttctc gtccagcacg ggctcgacgc cgaacagctt gccgtaccac gcgacgctgg
      361 cgggcaggtc ggagacggtc agggcgacgt ggccgatgct cgggaaggcg gtcatgagtg
      421 agctccttgt tgggggtcgt ggtcggtgaa cgttgccgcc ggtttcggcc ggcggtcgac
      481 ctccaggcgg aagatgtcgg gtcgggcgta gtggccgacc gggtcgaaca tgcgccggga
      541 caggcgcgcc gcggtgacgt cgacctgcgc gatgacgatg ccctcggcgc cgaccagcgg
      601 ctccgcgagc agctcgccct cggggccgac gacgcaggag ttgcccttcg acatccagtc
      661 ctcgtcgccc ccgtacagcc cgtcgcgtcc ctcgatgtcc gcggggacgt ccgagccgcg
      721 caggcagggc gtcacgccga cgacgtggca gcgcccctcc ttggcgatgt ggcgcagggt
      781 cggcacccac gtgtcgctgt tgtcccacgt cggcgcgacg tagatgtcga tgccctgcgc
      841 gtacagcgcg tagcgcgcca gcggcatgta gttctcccag cagatcagcg cgccgaggcg
      901 tccgatgggc gtgtccagca cgctgagggt ggaaccgtcg cccatgcccc acaccaggcg
      961 ctcgccaccg gtcggcatga ccttgcggtg cttgtgcgcg acgcgtccct gcgggtccag
     1021 ccacaccacc gtgttgtaga gcgtgccggc gtcccgttcg gtcacgccca tcgcgacata
     1081 ggcgccggcc tgctgcgccg cctcggccag gcgctgcgtc gccgggccgg ggatctccac
     1141 cgcctggtcg aacaggcgct cgtaccagcg cgacggcccg tccgcccacg gacgcgaacg
     1201 ccacacccag tccgggtagg tgggcacaaa ggattcgggg aacgcgatca gctgcgcgcc
     1261 gtcggccgcc gccttgccga tcaggtccac cgccttgtcg acggtcgcct cgcggtccag
     1321 gaacacgggc gcggcttgca ccgccgccac ggtcaccacg gtcactgcgg cctccgattc
     1381 atcgcggcac ccgggccggg cgctgttggt gatcctgcac cgccgggagg cgcgcgtaca
     1441 gacaattcgt cccacggccg ccgccacgcc cccgcccggt gcccgcaccg tccccggcgg
     1501 ggcgtatcgt ctggcggtgt ggcggtcgcc cgtggtcgcc gcgcggggag ggccgcatgc
     1561 gcgatgccgt cgtggagacc gtggtgggcg tcgaccggtt cccggacgat gtccgccgcc
     1621 tgacgacgct ggaccgcgtc gactacgcgg acctgttctg cgtcccggtc gatgacgcgc
     1681 ggcgcaggac gcccgaggag tgggcacgcg cgctgctgga ggagggcgcc atcgcccgcc
     1741 gctcggccag gcgcctgtgg acggcgctcg gcctgcggtt gggcccaccg ggctcgcccg
     1801 accacgtcca gggctggcgg atcgccgggc gcggcgacga ctggatcacg gtcgagaccg
     1861 gttcctggta catgagcggc caggccgtct gcaaggtcga cgccggccgg gtctcgatcg
     1921 cgctgttcct gcgccatgac aacccgctcg cgccggccgt gtgggctccc gtgtcggtgt
     1981 tccaccgccg cgccgtgccg gtgatgctcg cgcaggccgt cgccatcgtc gccggccgcg
     2041 gcggcggcgc ccaggaggcc gcctcctgag ggttgaatcc tggccatact gggtgccgcg
     2101 gccggcgggg ccgccggagc aaaggggttc accgtgaccg agaccgcgcc acgcaccgtt
     2161 gggccggagg ccctcgcccg cgccgccatg gccaacgtgg acgcgcacga tttcgccggc
     2221 ctgcgcgccg cgtggcaccc ggacctggag ctgtacttcg tcgccaccgg caagcgcgtc
     2281 ggccaggacg gcgccgccgc ggagttccgg gagatcttcg acgccttccc ggacttccgc
     2341 atcgccccaa cccgcgtgct cgccgacggc gacaccgcga tcgtcgaatg ggaggcccac
     2401 ggcacccaca ccggcgccgc ctaccagggc atcgcccgca ccggccggcg catcgagctg
     2461 cgcggcgtgg acctgatgga gttctccgac gggctgctgc ggcgcaacac cgtctactac
     2521 gacggcctgt ccgtggcccg cggcctggga ctgctgccgc cggacggcgc cgcgcaggac
     2581 cgggcgctga aggcgctgtt caacgtgctc acccgcctcc gcggcctggt ccggcgctga
     2641 cccgcacgcg gaccactctg gcgctgggcg cgctgtgcct ggcgggcgtg gcgacctacg
     2701 gcgcgcgcgg gtccctgggc ctgttcgtga cgccgtggga ggcccgcttc ggcgcctcgc
     2761 gcgccgacat cgccctggtg agcacggtgg gcttcctggt gttcgccgcc ggccagaccg
     2821 tggccggccg catccaggag tccgtgcccg gacgccggct ggtgctcgtc ggcgtggcgc
     2881 tgtgcgcgct gggctacggc ggcgcggcct tcgccccgtc gctgccggcc gtggtgctgc
     2941 tggtcggcgt gctggccgcc agcggcatcg cgctgtcggc gctgccggtg ctgagcgtgc
     3001 tggccgccga cctggtgcgc agccgcgagg gcatggtgtt cggcctgctc accgcggccg
     3061 gcgccggcgg ccaggtggtg gtgctgccgc tggccgccgc ggcgctgaac ggctccgagc
     3121 gcggtgcgct gctggtcatc gccggcgtgc tcggggtcgc cctggcgggc gtcgccctgg
     3181 cggtgccgcg cggcgccggc gtgcgccccg aggacgccgc gccgtccgtt ccggtccgcg
     3241 gcttcgcgcg gctgtggcgc gaaccgcgct tctggctgct gctggtgccg ttcttcgtgt
     3301 gcggctacac caccacgggc ctgatggaca cccacttcat cccgtacgcc gtggaccacc
     3361 atgtcccaga gaccgtcgcg tcgggcgcgc tggccacgct ggcggcgttc aacgtgctgg
     3421 gcgtgctggt ggccggcggg ctcaccgacc gtgtggaccg cggccgcctg ctggcgatcc
     3481 tgtacgcggg ccgggccggc agcctgctgc tgctgccgct gctgacctcg ccgcgcgggc
     3541 tgttcgtgtt cgccgtgctg ttcggcgtgg tggacttcgc gaccgtgccg ccgaccaccg
     3601 cgctgacccg ccgggtgttc cacaccggcg ggtgggcggt ggcgctcggc gtgatctccg
     3661 ccgcgcacca ggtcggctcc gcgctgggcg cctacctggg cggctggctg tacgaccgca
     3721 ccggcggcta cgagatcgtg ttcgcctccg ccgccgtggc gctgctggcc gccgccgcga
     3781 tgagcctgcg cctggcgggg gagcaggacc tcgccgaggc gcgtcgaacc tgatgggcat
     3841 gcatcgccgg gacttcctga aggccgccgg ggcgggagcc gtgctggccg cgctgccggg
     3901 cgtccagctc gccgcgcgcc agcccaccgg gacgctgctg gccggcgcgg cggtcgccga
     3961 catcacgccg gaactgggcg gggagttctt cggctacgtg cggcccgaca tccacgccga
     4021 aggggtcgcg ctgcgcctgc acgcgcacgc gctggtgctg gacgacggcg tgcgcaagct
     4081 ggcgctggtg tcggtcgacc tgggcgagcc gatggtcaag gccgaggtcg tcgaacgggt
     4141 gcgccacctg ggcttcgacg aggagaccgt gctgctggcc gcgacgcaca cccacgccgg
     4201 gcccaacgcc gccacggact ggaccgcctt ccagatcgcc gaggcgatcc ggctggccga
     4261 cgccgcccgg cggccggccc gcgccggctg ggcggtcgcg cacgtgccgg acgcgaacct
     4321 gtcgcgctcc gtggaggcgc acctggccaa ccacggccag gacctgttgc ccggcttcgg
     4381 cacaccggac ctggatccgg ccggcgccga ccacacgcgc gacaccgcgt tgcgcctgct
     4441 gcgggtcgag ggtgtcgacg gacggcccat cgccgcctgg acgcacttct cggtgcaccc
     4501 caccacgttc atcccgtcca acaagctgtt ctccgccgac ttctgccggg tcgcgaacct
     4561 gcgcttcgtg cagcgcttcg ccggcagtcc cgcgccggtg gcgatgtaca ccaacggcaa
     4621 ccagggcgac ctgatcacgt ggttcgacga ctacaaccag cacgccgtcg ccgaccgcat
     4681 cggcgcgcgc gtcgcccggg ccatgtcggc cgcgtgggac gacgcgggcc gcgcgctgtc
     4741 cagcgccgtc gtggtcgacg cgcgccgcac caccgtgacc taccagggcc aggaggtgga
     4801 gcccggcaag cgggtgggcg gccccgccgg cttcttcggc gcgagcttct tcggcggcgc
     4861 caagaacggc ccgtcgatct tctactggat gggcacggag gggaagcgcc gcccggccgc
     4921 cgccgccgac ccggtgcacg gccgcaagat cctggccgcg ccggcgccgt ggttgcccac
     4981 cgtggagctg caggcgctgc gtgtcggcga ccggctgctg ctgggcatcc ccggcgagcc
     5041 cagcgtcgag accggacggc gcgtccgcgc ggccgccatg gccggggcgc cggcggggct
     5101 ggcggacgcc gccgtggtcg gcctggccaa cggctaccac ggctacttca ccacgccgga
     5161 ggagtacgac cagcagcact acgagggcgg ccacaccgtg ttcggcaagt acacgagcct
     5221 gctggtgcag cacggccacg ccggcctggc gggggagctg gcggcgcgcg ggagggcgcc
     5281 cgcggactcc ggcgcgcccg ccgcgccggt cgccgcgccc gtgggcgacg gcgggaccga
     5341 cggcgccatc gtcgccgagc cgacggcgct gaccgagcgc atgcgcacgg tggagctgtc
     5401 ctggaccggc gggccgtcag ggcgcgaccg ccccgtgggc acgccgttcg tgcgcgtcga
     5461 gcggcgcgac gccgccggat gggccgccgt cgacgacgac ctgggtcacc gcatggtgtg
     5521 gttccagcgc ggccagacct acaccgcgcg ctgggacatc gaccccgcca tgcccaccgg
     5581 cacgtaccgc ttccgcgtga ccagcgcccg ctacgagctg acctcgcgcc ccttccaggt
     5641 gggcgtgtcg accgggctgc gcgtgcgcgg cgtcgagcgc gacggcccgc gcctggtgtt
     5701 ccgcgcgcag aacccgccgc cggacccgga gcgcggcctg ctcagccggc cgcgcacccc
     5761 cgccggcggg acgctgcgct tcacccagga cggccgggag cgcgtcgcga cgtgggacac
     5821 cgccgcgcgc ggctgggtgg ccgccgtggc gccaggcggc gggccggtca ccgtgccggc
     5881 cggtggactg ctggacgggc tgggcaaccg caacggccac gaggcggtgc tgcgcgccgg
     5941 cgaggtcgcg gccgtggact ggccggcgca catcggcccc gccggcgggc agacgcccgg
     6001 cccgttcggc gagggcacct tcccgcccta gctcccgccg tcgcggcaag gcccgcgcgc
     6061 ctcagtgggc ggcgccgtcg cccttgtccg ccggcagcac gtcgcgctcg gagggatgcc
     6121 acaccgggct gtcgccggcc gtctccacgt tgaagccgta gtcgtagacc agcgaaccgc
     6181 cgatcgtgcc gcccagcgcg gtgaggccgg ccaccaccaa tgtcagcgcc aggacgatcc
     6241 cgggcgtggc ggccgcggcc ccataggtgc cggcgcgcag gacgagctcc acgaccacca
     6301 gcacggtgag cgccgccatc gtggccgcgt gcgcgttgac ggtgcgccac gcctgcgtgc
     6361 ccttggacgt cgaccccacc cagtcggcca cgccggtgat cgcggcgagc accgccacca
     6421 gcaggccgcc ggccagcacg aacgtgcccg cccggtacag ctcgcgcgcc cacggctcgc
     6481 cgtcgcccag gaacgagatc acgtcgaacg ccgccgccag gatgtacgcg cccaccggca
     6541 cgtcggtcag cggcgggtgc agcggcttgc cggccaggcc gcgcaggccc ttgggcttgc
     6601 ggccgcgcac ggacaggctc ggccggaacg agaacagtcg catctggggc ctccttttga
     6661 cgtcgggtgg tgctcagtcc gcccgcacca gggacaagaa ctccgccctg gtgcggtggt
     6721 cgtcgcgcag cagtccccgc accgccgagg tgaccgtgcg ggcgcctggc ttggccacgc
     6781 cgcgcagcga catgcacgag tgcgtggcct ccagcaccac gccggccccg cgcggcgcca
     6841 ggcgccgctc cagctcgtcg gcgaccgacg tggtcagggt ctcctgcacc tgcaggccgc
     6901 gcgcgtggaa ctccaccacg cgcgccagct tggacaggcc gacgatgcgc tcaccgggca
     6961 cgtagccgac gtgcgcgacg ccgctgaacg gcagcaggtg atggcggcac agcgagtgga
     7021 accggatgtc gcgcaccagg accagctcgt cgtagcgccc gtcgttgggg aacgtggtga
     7081 acgtgaacgg cgtcggcgtc agcagttcgt ccagggccgc cgccacccgg cgcggcgtgt
     7141 cgcgcagatc ctccacgtcc gggtcggcgc cgaccgcgac cagcaggtcg cgcacggcgc
     7201 gggccgcggc cacgcggtcg aaggcggggg cgtccggcgc ccgctgctgc acggcactgt
     7261 cctgcgcaca cgtctgcatg gcccgcagca gaccacaggc cggctactaa agtcaacaaa
     7321 gataggtttt agaatcggat gaccacgcag cactcgcccg ggcgcgggtc cagcgccagt
     7381 tccggcgcca gcgccagcgc cccggcggcg ccggccacga actcgccggt catgccgcac
     7441 accagctcgg tgtgggccga cgccagcggg tggaacgggc agttgcgcac gcgccaggcg
     7501 ccgccgtcct ggtagggctc gtagccgcag tccgacagca ccgcccacag cgcctccagc
     7561 cgcctggccc gcgacgggcg cgaccccagg ccggcggcga gccgcgccgc ctcggccctg
     7621 ccgcgctccc gggccgcgtc cgccagcgcg ccgcgcgcgg gaccggcctc cgggcggtcc
     7681 agcgcgtcgg ccagcagttc cgcggccaga cggaagcgcc ggggcggcaa ggtcacgtcc
     7741 acctggcgct cggcgcgccg gtacagcttc gccgggcgcc ccgcgcccgg gccgctgcgc
     7801 ccgctgagcc ggcggtactc cacctcgagc agcccggcct ccgccaggcg gtccaggtgg
     7861 aacgccgcca ggccgcggga cacgtcgacg gccgcggcgg cctcgtcgcg gccgaccgcg
     7921 tcaggccggc cgaccacgta gcggtacagc gcgcggcggg tcggctcgtc caggagcgcg
     7981 atatcgtcca gctggtcctc gaggtcgtcg cgtgcgtccg tcatgccacg cacgatgccc
     8041 tatcggcaat gatcgtgcac gttagaagcc gttgtgcggt tcggcccgcc cgcccccggg
     8101 catggccagg cacagggtcg gcaacgtcgc aaggaggtcg catggaaccg ggcccactgg
     8161 tcatcggcgc cgagcgcgtc cgtaccgggc agaccatcga ggtcacctcg ccctacagcg
     8221 gcgaggtggt gggcgcggtg gccaaggccg gcaccgagca cctcgacgcc gcggtggcgg
     8281 ccgcccggga cgccatgtcg gccccgctgc cgacccacca gcgcgtcgcc gtgctggacg
     8341 cggtcgcggc gcggctgacc gagcgcgcgg aggacttcgc ccgcctgatc gccgccgagg
     8401 ccgccaagcc gctgcgcacc gcgcgcgccg aggtggcgcg cggcatcgac acgctggcgt
     8461 tctcggccgc ggccgcccgc accctcaccg gcgagttcct gcccctggac gcctccgccg
     8521 ccggtgaggg caaggtcggc tgggtcacgc gcgtgcccgc cggcgtggtc gccgcgatca
     8581 cgccgttcaa cttcccgctg aacctggtgc tgcacaagct ggggccgtcg ctggcggccg
     8641 gctgcgcggt ggtgctcaag ccggcctcgt ccacgccgct gaccgccgcc gcgctggtcg
     8701 agctgttcca cgactgcggc ctgccgcccg ggtggctgaa cttcacgccg tgctcgggct
     8761 ccaccgccaa ccacctggtc acccacccgg gcgtcgacgt ggtgtccttc accggctcgc
     8821 gcgaggtggg ctggtcgatc cgcgagcagg cgccgcgcaa gcgcgtcggc ctggaactgg
     8881 gcaacaactc gccggccatc gtggaaccgg acgccgactg gcgcgcggcg gcgcgcaaga
     8941 tcgccgccgc gggcaactcc tacgccggcc agtcgtgcat ctccacgcag cgcgtgtacg
     9001 tgcacgacga gatcgccgcc gccttcctgg acgagctggc ggccgcggtg ggggagctgg
     9061 tggtcggcga cccgctggac gaggcgaccg acgtgtccgc gctgatcgac gcgggcgaga
     9121 ccgagcgcgt ccgcgcgtgg gtgcgcgagg cggtcgccgc gggcgcgagc gtcgccgccg
     9181 ggggcgagca ggacggtggc gtgctgcgcc ccacggtgct cgccgacgtg aagcaggaca
     9241 tgcgcgtgtc gcgcgaggag gtgttcggcc cggtgctggc ggtgcagacc tacggcgact
     9301 acgacgacgc gctggccatg gccaacgaca ccgactacgg gctgcaggcc gccgtgttca
     9361 cccgcgacct cggcaaggcg ctgcgggccg cgcgggtcct gcgctacggc ggcgtgctgg
     9421 tcaacgagac gccgacgtgg cgcgccgacc tcatgccgta cgggggcgtg ggcgactccg
     9481 gcaacacccg ggagggtccg gcgtacgcga tcagggaact gctcaccgag cagcgcctgg
     9541 tggtgctgca accctgaccg ccccgacacc ggaggatccg tcgacaatga agggcaagcc
     9601 ctacgggacg ctggcgctcg cgctcgccgt ccacctgctg atcatgttcg cgctcacgta
     9661 cgcgggcgtg taccggctgg atcacgtgta cgcgaacctg aaccgcttct acatggcgct
     9721 gctcatggtg gcgcccatgg cgatcgtgat ggtcgcgttc atgtggcaca tgttccccag
     9781 gcgcgcgctg aacgtcgcca tcctggggct ggccgtcgtg ctgttcgccg ggaccttcgc
     9841 cgccatccgg gcgcaggcgc tcgtgggcga catgcagttc ctgcgctcga tgatcccgca
     9901 ccactccatc gccataaaga cgtgcgagcg agcccagatc tccgatccgg agatcacgaa
     9961 gctgtgcgag cagatcgtgc gcgcccagcg cgaggagatc gcccagatgg aggacatcat
    10021 ccggcgtctc gactgatgcc cgcctggcgt gttcaatccg cctccaaggg tgcgagtgac
    10081 gtgggaaggt ggggacatgc ggcgcgcggt gaccggcggg atcctggcga gcctgctcgt
    10141 gctgctcggc ggcgcgcacg cggggctggc ccaagagccc gggtacgcgc cgctggacca
    10201 gcccggcccg gcgctgcggg tccccctggc ggagctggag gcgagcctga cctgcgacgg
    10261 cgcgctggac ggctcgcgcg cggccgtgct gctgatccac ggcaccaacc tggactacga
    10321 gaccaacttc ggctggaact accagcgcca gttcgccgcg ctgggctggc cgcactgcgg
    10381 cgtcgacctg cccaagaagg gcatggacga catccaggtg gccgccgagc acgtcgtcca
    10441 cgcggtccgc accgtcgcgg cgcggaccgg gcgccccgtg cagatcgtcg gcttcagcca
    10501 gggcggcatg atcgggcgct gggcgctgcg cttctggccg gacacgcgcg acctggtgga
    10561 ggaggtcgtg gggctggcgc cgtccaacca cggcacgctc accgccatcc ccacgtgcaa
    10621 cccgtcgtgc gtgccggcct accagcagca gcgcgccgac tcgcgcttcg tcgcggcgct
    10681 gaactcgcgc gccctgacct tcggcggcgt cgactacagc gtgatctaca cgcgcacgga
    10741 ctgggtcgtc acacccaacc acgacggcag cgcgtcgtcg ctgcccgccg ggccgaacgc
    10801 ggtcaacgtc gccatccagg acatctgccc cggcgactcc tccgagcacc tgcacctggg
    10861 cagcttcgac gcggtcggct acgcgctggt gcatgacgcg ctgacccacg acggccccgt
    10921 ggaccccgct cgcatcgcgc ccacggtgtg cgccgagccg tacatgccgg gcgtggaccc
    10981 cgccacgttc gccggggact acgccgccta cctgcgccag gtcggcgagg cgtaccagga
    11041 gacggaggcc gtgacggagg agccaccgct ggcctgctac gtgaccgcgt cctgcgccga
    11101 gcccgccggc cagccgccca cgggggccga ggcgccgccc gccgcccctg cgctgcccgc
    11161 caccggcggc ggcggcctcg ccggtgcggc gctcctggcg gccgccgccg cgtccgtgcg
    11221 acgccttcgc cggtgaccgg cccggcccgg ccacagggtc cgctcgggag gacgcgcgtt
    11281 gggtgacttg gcgatggaca cggcggtcga gccgcgcggg gagggccggt tcgccgccca
    11341 gctctcgcag gactgggaga tctgggggcc caacggcggt tacctggccg ggatcgcgct
    11401 gcgcgcagcc gcggcgtcgt cggcgcagcc ggtgccggcg tcgctgacct gccatcacct
    11461 gtcggtggcc tccttcgacc cggtggagct ggaggtggtc tccctgcggg cgacacgccg
    11521 cgccgagtcc ctgcgcgtga cgatgtggca gcaggacaaa cgcatcgtcg agtccctggt
    11581 gtggctggtg acgccgggcg acgggctcct ccacgatgcg gacacgcccc cggatgtcgc
    11641 ggatccggac gccttgccga gcctgccgga actgctgggc gccgagcggc gcaaccctcc
    11701 gtcgtatcgc ttctgggaca atttcgaggc gcgcccgctg gactggaacg gaccgttcga
    11761 gcagcggccg gccggtgagc cccgcgtgcg caactggtac cgctacacac cgacggccac
    11821 gttcgacgac ccggtggtgg acgccatccg ctcgctgatc ctgctggaca ccatgagctg
    11881 gccggccgcg cgccgcgccc acccgcccgg gccgctgccg tacgtggcgc ccacgctgga
    11941 cgtggcggtg cagttccacc gcgcggcacc ggcctccgag tggctgctgg tcgagggcgt
    12001 cgcgccggtg gccgaacagg ggctggccgc gtacagcgcc aaggtgtggt cgcgggaccg
    12061 gcgcctgctg gcctccgccg ccggccagat gctgtgccgg ccggtgccag ggtgagcgcg
    12121 cgcggcatgg ccgcgtgggt gctcgccttc gcgctggcgg cgtgcaaccc gggcttcgcg
    12181 ccggagccga cgccgcggcc gagcgtgccg gactacgagg ccgagggcat gcccaccccg
    12241 gcgccgctgg accaggccgc cgtgccggtg gtcgcgctgg ccggcctgcc gcaccaccgc
    12301 gtcgacgtgc cgggcgggcc cgactggctc gcccaggacg cggaccgggg cgggatcttc
    12361 gtcaaactgg accgcggcga cgtggccgag ctcgacccgg cgaccggggc ggtgctccgc
    12421 accctcggcg cgcccgcgcc gctgtgccag ggcatcggcg ccggtttcgg cggcgtgtgg
    12481 acgtgcggcg gcgcgggcgt gcgccggctg gagatcgcgt ccggcgagca gacccgcgac
    12541 cttcccgtcc gcaaggcgtt cgagcagggc catctgcccg ccgccttcgg gcaggtgtgg
    12601 gtgctcaccg ggaccggcga ggagctcgtc ggcctgaacc ccgcggacgg ctccgtggcc
    12661 accaccgtcg cgctcgacgc gcgcgggcac gacctggtgt tcgcccccga ggcgctgtgg
    12721 gtggtcagcg ggcccgacga cctggtgctc ggcctgtacc cgaggcagga cgtcgtggcg
    12781 atgcgcatcg gcggcatcga caacccgcgt gccgtggcgg tcgcccgcga cgtctgggtg
    12841 gccgctccct ccggggtcta ccgcatcgac cgcaccacgg gtgcgctgct cggcgcgatc
    12901 ggcctgccgc tgggccagga caccgcgatc gccgccgacc ccaacgacgt gtgggtgcgc
    12961 agcgccgccg gcttcctgca ccgcatcgac gcgcgcacgg gcgaggtggt cgagcgcatc
    13021 gacgccggga tcgcgggcgg cggcgacgtg ctgctcaccc cggacgcggt gtgggtgacc
    13081 gcctacgacg cgaacgtggt ggtgcgcctg gcccggcagc gctgagccgc tggcggcgca
    13141 ggcactgcga cgcgttgggc gggggagggc ggacgcgcgc cgagggtaag ccgaccggct
    13201 ctgcgcggca gggccggtgg actctgcgcg tcggcacgcc gcgggcgcac catgcggcca
    13261 tggcgccggc aggcgccgga atccgagtgc gaacggaggt ggaggcgcgc gccgggcttc
    13321 tctgccagcg acacggagct ccggcgcgcg ggcccccacc tcacacagga ggcatgacga
    13381 tgctttccct caccgacgcc gcccagcggt tcctgtcacg ccggcgcatc gccgtcgcgg
    13441 gcgtgtcccg gacgccgcaa gggcacggcg cgaacgtggt ctaccagcgg ctgcgccagc
    13501 gcggctatga cgtcttcccg gtcaatccga acgccgagga ggtcgaaggc gaccgctgct
    13561 acccggacct gcgctccatc cccggcggtg tcgaggcggt cgtgatcggc accgccccgg
    13621 cggccgccga gggcctcgtc cgcgagtgcg tcgacctggg tgtcaccgac gtgtggatgc
    13681 accgctcctt cgggggcggc agcgtgtcct cgtcggcggc gcgctacggc cgcgagcacg
    13741 gcgtcatggt gatcgagggc ggctgcccgc tgatgttcga gcccaccggc gacgggccgc
    13801 accgcttcct caagttcgtg tgcacgttgg ccggcacggt cccgcgcacg gtcgaggaga
    13861 tctcgccggc ggcgccggcg agcggctgac ggcttcagcg tcgcggccgc aggcgcacga
    13921 tgcggtcgtc ctgcggcccg gggctgccgc gcccgtcggt gttgttcgtg gcgacccaca
    13981 ggctgccgtc gggcgcctgc gcgacggcgc gcagccggcc g
//
LOCUS       DATMKC010000014         8822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_310404, whole genome shotgun sequence.
ACCESSION   DATMKC010000014 DATMKC010000000
VERSION     DATMKC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310404"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..530
                     /locus_tag="VNU01_00490"
     CDS             147..530
                     /locus_tag="VNU01_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31126.1"
                     /translation="MAVSVVTDVALAGARAQAAADAAALAAAGATLLGPAPASPRAAA
                     VQAARLHGARLLECCGGAGVVPPRIRVAVAVAPASGLGRALRGSVVRHAAASLRPAKA
                     PGGPSGFAVIVPNSQGNGAGTRPRR"
     gene            629..1345
                     /locus_tag="VNU01_00495"
     CDS             629..1345
                     /locus_tag="VNU01_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWH31127.1"
                     /translation="MPDPFGGKEPSGGGAVSYLLDSLRLRPAGRRALSLLSVLLFLVG
                     AGMFAYPFVTDIYAEQVVQSGLEDTFDSAEFRQAYEAGELKAGDPLTRIHIPKIGVAA
                     VVVNGTSPSALRAGAGHYPNTPLPGAQGNVGIAGHRTTYGKPFNRVDELLVGDEIRLE
                     TPIAIHTYRVLAPPADVATPCDKDRRLGCWITHPADWSVVAPMEGYFLTLTSCHPKGS
                     ARQRIIVRAELVESVARPTG"
     gene            1384..2493
                     /locus_tag="VNU01_00500"
     CDS             1384..2493
                     /locus_tag="VNU01_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31128.1"
                     /translation="MTRTGATAVVRLACALLLAAAVVLLPAGGARAAGEPGLERPTPS
                     ERVTQDGPYQITGYVDAGPGEEVRAMEARLLFDGTQLGSVRNMDFIGSQETGPGMRRT
                     RWAVALDPLRYWEDAQPLLNGRYRVEVRATVVFQSVPREPTAWRGVDFVIDVDPPATT
                     VQAKVLDAAQRRVEVSWEPVLLPDFVRYVVQRKIGAGDWKDYREVPSAEGTRWADVVP
                     GDGEFAYRVQTYRRAAGDGERKSPWSAPANVSVRKPPPPAPPSESGEGGAGAAAEQGP
                     PVLRVRTPPPARPPAFTGPDTYEETLDYGDTEVPLTSGPEQDLAQDEAAEELGELRVR
                     EDRDFPVRQALVPVAAGLVLTVSAWHLRRFLRVSA"
     gene            complement(2495..4783)
                     /locus_tag="VNU01_00505"
     CDS             complement(2495..4783)
                     /locus_tag="VNU01_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWH31129.1"
                     /translation="MAADPDTMLAQLDAALRESMPAADGRPRAAHVHHLPARAPRTVP
                     LPDDLPALLAQRLALAGVESLYVHQAEALELARARRHFVVATGTASGKSLCYQLPLLE
                     QVLADDRSTAIYLAPTKALAHDQLRALRAFRIPHVRAAAYDGDTPRQERDAIRRTANV
                     LLTNPDMLHAGLLPQHKRWADFFHRLRLVIVDECHVSRGVFGAHVAAILRRLRRLTEH
                     YGADPLFVFASATIGNPGEHAGNLSGLDVAEVVRDGAPKPRVAMALWEPPLTDERSGA
                     RRSTLAEAADVLAGLVEGGLQTLCFVKSRKGAELIAAHARRILDADDVLAYRAGLLPE
                     ERRAVEQGLIAGGVRGVAATDALELGVDIGSLDAVVIAGWPGTTASLWQQAGRAGRRD
                     REAVVVFVAEDNPLDHYLLANPVQLWERPLEHATADVTNPYILAPHLDCAAWELPLHA
                     EERAFAPAEIAPFLAEAVADGRLRVRGESHYWTGRGSPTSGVSIRSAGGEPFAIVEAG
                     TGALIGDVDEARAYRTVHDGAVYLHQGRTFRVRSLDLKDHVALVERYDGEEYTQPRAD
                     TDIAVLALRESVAWGRCRMHLGRVEVSTRVLAYERKRLMTNESLGLVDLDLPEQRLVT
                     QALWLTIPEAVLDLAGLGAERVPGAAHAAEHAAIGLLPLFAMADRWDIGGVSTPWHPD
                     TGEATIFIYDGYPGGAGIAERGYRSDDAHLRATLAAVASCRCADGCPSCVQSPKCGNG
                     NHPLDKAGAIALLEAVLSERRA"
     gene            4937..5275
                     /locus_tag="VNU01_00510"
     CDS             4937..5275
                     /locus_tag="VNU01_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWH31130.1"
                     /translation="MELTLNHRKEGDRTVLEVGGEVDVYTAPKLREKLVELVGEGHYH
                     IVVDMTKVEFLDSTGLGVLVGGLKRVRSHDGNLALVCNQERILKIFRITGLTKVFPIH
                     ETLDEALQDA"
     gene            5275..5715
                     /locus_tag="VNU01_00515"
     CDS             5275..5715
                     /locus_tag="VNU01_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH31131.1"
                     /translation="MDLAELHLPRSVAYVGLARSIVASAARLAGLGGDRLEDLKIAVS
                     ESATHAVTAAQRDAEPLVLAFGPHEGAFEVHVLSPEPARRDPAVEALREWPDEGGLGL
                     TLVEGLADAVLFPDSAHHQMVLRFDLPARTDVPEQARRSSGAPR"
     gene            5829..6443
                     /locus_tag="VNU01_00520"
     CDS             5829..6443
                     /locus_tag="VNU01_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWH31132.1"
                     /translation="MDPRTKSSLAAYEKSAVEYQEFWRQRRPLDAVRKFAALAGKGAT
                     VLDPACGPVLDVRLLRDAGLRVVAGDLTHEVMKLGKMLFPKGALARWDLRNLPFLDGS
                     FGGVWAPAALQHFPRGEILPALRELRRVHREGPIFLSFRQGAGDLEEVEDPPAGTVYA
                     TTVSADELKALLLGVGYTEVEVEQRPDPLERRDVTWLYGWGRLK"
     gene            complement(6460..6993)
                     /locus_tag="VNU01_00525"
     CDS             complement(6460..6993)
                     /locus_tag="VNU01_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31133.1"
                     /translation="MAQTVGSPATQASGRSSESALMVAATFFAAVILLHNFDHVRRGA
                     DTAGLDVLVIGTGAIVLEIAVVILCVQRHRLAALAAAWSGLVLAVGYLIVHFTPRRTW
                     LSDSLLEGADTLSLVAATLEVAAAAALAITGFLVLRARPSSQASSSPLARALVHPVAA
                     VTIAGNTAAIAIAFAQR"
     gene            7149..8162
                     /locus_tag="VNU01_00530"
     CDS             7149..8162
                     /locus_tag="VNU01_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWH31134.1"
                     /translation="MSLADRALGRLACAGRQLPNPHILIRPYLVREAVASSRIEGTQA
                     SLSEVFDAEAQEVEATGDLLEVANYIRAMELGLRLVQDVPISTRFIRELHKTLMAGVR
                     GQERSPGEIRTSPNWIGSPDNRPETAVFVPPPVDEMHAALSDWERFLHYPPPLPVLVR
                     CALLHYQFETIHPFLDGNGRIGRLLIVLFLVEQGDLPQPLLYTSAYFEEHKADYYGRL
                     QGERGELEEWIQFFLTAVARQAQDALQRAERLADLREGYRQRLAVGRSRAHEVIDLLF
                     ANPVMTAKFVARGLGITSQGAHNLLGRLKEAGVVEELPRVPGRSLRWQAREILEALGG
                     FAS"
     gene            complement(8219..>8822)
                     /locus_tag="VNU01_00535"
     CDS             complement(8219..>8822)
                     /locus_tag="VNU01_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWH31135.1"
                     /translation="FKAFEQAIQAAGGSISLDIGDVAVFSGLFLGAMLPFLFAALTIN
                     AVGRAANKMIEEVRRQFREIPGLREGKEGVVPEYAKCVDISTGAALKEMLIPGALAVV
                     VPLVVGFVSVDALGGFLAGALVSGFALAIFMANAGGAWDNAKKYIEAGNHGGKGSEPH
                     KAAVVGDTVGDPFKDTSGPAMNILIKVMTIVSLIFASAFV"
BASE COUNT         1207 a         3141 c         3258 g         1216 t
ORIGIN      
        1 tcagggtgcc gcaggcggcg gcgcccacac cgccgatcat ggcgtactcg gccgtctgcg
       61 agccgcgctc ctggcgcagc cgggacatgc ggtcgcggag caatcgggcc atgtgaccct
      121 cctttcgttc gtgcgccgcc gggctggtgg ccgtcagcgt ggtgacggac gtcgcgctgg
      181 ccggagcccg cgcgcaggcg gcggctgacg cggcggcgct cgcggcggcc ggggcgacgc
      241 tgctggggcc cgcccccgcg tccccgcggg ccgcggccgt gcaggcggcg cgcctgcacg
      301 gcgcgcgcct gctcgagtgc tgcggcgggg cgggcgtggt gccgccccgc atccgggtcg
      361 cggtggcggt cgcccccgct tccgggctgg ggcgggcctt gcgtggcagt gtggtgcgcc
      421 atgcggccgc ctcgctgcgt cccgcaaagg cgccaggcgg gccgtcaggt ttcgcggtga
      481 tcgtgccgaa cagtcaaggc aacggggccg gtacacggcc tcgacgctag aatccggaca
      541 acaacgtcat ctcggcgagg cccgcccctt catggccgac gcaagctcag tcacgacgcg
      601 caccgccccc tcggagggcg gcctcgtcat gcccgatccg ttcggcggca aggagccgtc
      661 gggcggtggg gccgtctcct acctgctcga ctccctccgg ctgcggccgg ccgggcgccg
      721 ggcgctgtcg ctgctgtcgg tgctgctgtt cctggtgggc gcgggcatgt tcgcctaccc
      781 gttcgtcacc gacatctacg ccgagcaggt ggtgcagagc gggctggagg acaccttcga
      841 ctcggcggag ttccgccagg cctacgaggc cggcgagctc aaggcgggcg acccgctcac
      901 gcgcatccac attcccaaga tcggggtcgc cgccgtcgtg gtgaacggga cctcgccgtc
      961 ggcgctgcgc gcgggcgcgg ggcactaccc caacaccccg ctgccgggcg cgcagggcaa
     1021 cgtcggcatc gccgggcacc gcaccacgta cggcaagccg ttcaaccgcg tggacgagct
     1081 gctcgtcgga gacgagatcc gcctggagac gccgatcgcg atccacacct accgcgtgct
     1141 ggcgccgccg gcggacgtgg ccacgccgtg cgacaaggac cgccggctgg ggtgctggat
     1201 cacccacccg gcggactgga gcgtcgtggc gccgatggag gggtacttcc tgaccttgac
     1261 cagctgccac ccgaagggct ccgcgcggca gcgcatcatc gtgcgcgcgg aactggtgga
     1321 gtccgtggcg cgtccgaccg gctgacagaa gtgacgacaa cagtgggaag cgggccagca
     1381 cgcatgacca ggaccggtgc gaccgccgtc gtgcggctgg cctgcgcgct gctgctggcg
     1441 gccgcggtgg tcctgttgcc cgccggaggc gcccgcgcgg ctggcgagcc cggcctggag
     1501 cggcccaccc ccagcgagcg cgtgacgcag gacggcccgt atcagatcac cggctacgtc
     1561 gacgccggcc ccggcgagga ggtccgcgcc atggaggcgc gcctgctctt cgacggcacg
     1621 cagctgggct cggtcaggaa catggacttc atcggctcgc aggagaccgg gcccggcatg
     1681 cgccgcacca ggtgggcggt ggccctggac ccgctgcgct actgggagga cgcgcagccg
     1741 ctgctgaacg gccgctaccg cgtcgaggtg cgcgccaccg tggtgttcca gagcgtcccg
     1801 cgcgagccca ccgcctggcg cggggtggac ttcgtgatcg acgtcgaccc gccggcgacc
     1861 accgtccagg ccaaggtgct ggacgcggcg cagcgccgcg tcgaggtgtc ctgggagccg
     1921 gtcctgctgc cggacttcgt gcgctacgtg gtgcagcgca agatcggcgc gggcgactgg
     1981 aaggactacc gcgaggtgcc cagtgccgag ggcacgcgct gggccgacgt ggtgcccggc
     2041 gacggcgagt tcgcctaccg cgtgcagacc taccgccgtg ccgccgggga cggtgagcgc
     2101 aagtcgccgt ggtccgcgcc ggccaacgtc agcgtccgca agcccccgcc gcccgcaccg
     2161 ccgtccgaaa gcggtgaggg cggcgccggc gcggcggccg agcagggccc gccggtgctg
     2221 cgcgtgcgca cgccgccccc cgcgcgcccg cccgcgttca cgggcccgga cacctacgag
     2281 gagacgctgg actacggcga caccgaggtg ccgctgacca gcgggccgga gcaggacctg
     2341 gcgcaggacg aggccgccga ggagctgggc gagctgcgcg tgcgcgagga ccgcgacttc
     2401 ccggtgcgcc aggcgctggt gccggtggcc gccgggctgg tgctcacggt gagcgcctgg
     2461 cacctgcgcc ggttcctgcg cgtctccgcc tgactcaggc gcggcgttcc gacagcaccg
     2521 cctccagcag cgcgatggcg cccgccttgt ccagcgggtg gttgccgttg ccgcacttcg
     2581 gcgactgcac gcaggacggg cagccgtccg cgcagcggca ggacgcgacc gccgccaggg
     2641 tggcgcgcag gtgcgcgtcg tcgctgcggt agccgcgctc ggcgatgccc gccccgccgg
     2701 ggtagccgtc gtagatgaag atcgtcgcct cgccggtgtc ggggtgccac ggcgtggaca
     2761 ccccgccgat gtcccagcgg tcggccatgg cgaacagggg caacaggccg atggcggcgt
     2821 gctcggcggc gtgggcggcg cccgggacgc gctcggcgcc cagccccgcg aggtccagca
     2881 ctgcctccgg gatggtgagc cacagcgcct gcgtgaccag ccgctgctcg ggcaggtcca
     2941 ggtccaccag gcccagcgac tcgttggtca tgaggcgctt gcgctcgtac gccaggacgc
     3001 gggtggacac ctccacgcgt cccaggtgca tgcggcagcg cccccacgcc accgactccc
     3061 gcagcgccag caccgcgatg tcggtgtccg cgcgcggctg ggtgtactcc tcgccgtcgt
     3121 agcgctccac cagggccaca tggtctttca ggtccagcga ccgcacgcgg aacgtgcggc
     3181 cctggtgcag gtacaccgcg ccgtcgtgca cggtgcggta ggcgcgggcc tcgtcgacgt
     3241 cgccgatgag cgcgcctgtc ccggcctcga cgatcgcgaa cggctcgccg ccggcgctgc
     3301 ggatgctgac cccggaggtg ggcgagccgc ggccggtcca gtagtgcgac tcgccgcgga
     3361 cgcgcaggcg gccgtcggcc accgcctcgg ccaggaacgg cgcgatctcg gcaggcgcga
     3421 acgcgcgctc ctccgcgtgc agcggcagct cccaggcggc gcagtccaga tggggcgcga
     3481 ggatgtaggg gttggtcacg tcggcggtcg cgtgctccag cggccgctcc cacaactgca
     3541 ccgggttggc gagcaggtag tggtccagcg ggttgtcctc ggcgacgaac accaccaccg
     3601 cctcgcggtc gcgccgcccg gcccggccgg cctgctgcca cagcgacgcc gtggtcccgg
     3661 gccagccggc gatcaccacc gcgtccagcg agccgatgtc gacaccgagc tccaaggcgt
     3721 cggtggcggc cacgccgcgc acgccgcccg cgatgaggcc ctgctcgacg gcccggcgct
     3781 cctccggcag cagcccggcg cggtaggcca gcacgtcgtc ggcgtccagg atgcgccggg
     3841 cgtgcgcggc gatcagctcc gcgcccttgc gggacttcac gaagcacagc gtctgcaggc
     3901 cgccctcgac caggccggcc agcacgtcgg cggcctcggc cagcgtcgag cgcctggcgc
     3961 ccgagcgctc gtcggtcagc gggggctccc acagcgccat ggccacgcgc ggcttgggcg
     4021 cgccgtcgcg cacgacctcg gccacgtcca ggcccgacag gttgccggcg tgctcgcccg
     4081 gattgccgat ggtcgcgctg gcgaagacga acagcgggtc ggcgccgtag tgctcggtga
     4141 ggcgccgcag gcgccgcagg atcgccgcca cgtgcgcgcc gaagacgccg cgtgagacgt
     4201 ggcactcgtc cacgatgacc agccgcaggc ggtggaagaa atccgcccag cgcttgtgct
     4261 gcggcagcag gcccgcgtgc agcatgtccg ggttcgtgag cagcacgttg gccgtgcgcc
     4321 ggatggcgtc gcgctcctgc ctcggcgtgt cgccgtcgta ggcggccgcc cgcacatgcg
     4381 ggatgcggaa ggcgcgcagc gcccggagct ggtcgtgggc cagggccttg gtgggcgcca
     4441 ggtagatcgc cgtggagcgg tcgtcggcca ggacctgctc cagcagcggc agctggtagc
     4501 acagcgactt gccgctggcc gtgccggtgg cgaccacgaa gtgccggcgt gcgcgggcca
     4561 gctccagcgc ttcggcctgg tgcacgtaca gcgactccac gccggccagg gccaggcgct
     4621 gggccagcag cgccggcagg tcgtcgggca acggcacggt cctgggggcg cgcgcgggca
     4681 ggtggtgcac gtgcgcggcg cgggggcgcc cgtccgcggc cggcatgctc tcgcgcaggg
     4741 cggcgtcgag ctgcgcgagc atcgtgtcgg ggtcggcggc catggcgggc gccaggctag
     4801 ccgggtgccg cgacggggta ggctctttgc gcttttggat gatttgcgtt tcggctgtgc
     4861 cggtcgaggg cacgtttgcc tgcgcttgcc gcttgcccgc gtctgcgcgg gccattttcg
     4921 gtcgaggagt gtcggagtgg aactcacact gaaccaccgc aaggaaggcg accgcacggt
     4981 tctcgaggtg ggtggcgagg tcgacgtgta cacggcgccc aagctgcgcg agaagctcgt
     5041 ggagcttgtg ggcgagggtc actaccacat cgtcgtggac atgacgaagg tggagttcct
     5101 ggactcgacc gggctgggcg tgctggtcgg cggcctcaag cgcgtccgct cgcacgacgg
     5161 caacctggcg ctggtgtgca accaggagcg catcctgaag atcttcagga tcaccgggct
     5221 caccaaggtg ttcccgatcc acgagacgct ggacgaggcg ctgcaggacg cttgatggac
     5281 ctggccgagc tccacctgcc gcgcagcgtc gcgtacgtgg gactcgcccg gtcgatcgtc
     5341 gcctcggccg cccggctcgc gggcctgggc ggtgaccgcc tggaggacct caagatcgcc
     5401 gtgagcgagt cggcgacgca cgcggtcacg gcggcccagc gagacgccga gccgctggtg
     5461 ctggcgttcg ggccgcacga gggcgccttc gaggtccatg tgctgtcgcc cgagcccgcc
     5521 cgccgcgacc ccgcggtcga ggcgctgcgg gaatggcccg acgagggcgg cctggggctc
     5581 acgctggtgg agggcctggc cgacgcggtg ctgttccccg acagcgctca ccatcagatg
     5641 gtgctgcgct tcgatctgcc cgcgcggacc gacgtgccgg agcaagcgcg gcgctcctcc
     5701 ggcgcgccgc gctgaaatca gtcggcgttt ccgcgcgtcc ggctcctccg gcttcgctac
     5761 catccgatgc gggcccgtgc ttccccaccg ctcgcggtgc cctgacaccg acacgaggac
     5821 gggtgcgcgt ggatccgcga acgaagtcga gccttgcggc gtacgagaag tccgcggtgg
     5881 agtaccagga gttctggcgg cagcgccggc ccctggacgc ggtgcgcaag ttcgccgcgc
     5941 tggccggcaa gggcgccacg gtgctggacc cggcctgcgg cccggtgctg gacgtgcggc
     6001 tgctgcgtga cgccggcttg cgcgtcgtgg cgggcgacct gacccacgag gtcatgaagc
     6061 tgggcaagat gctgttcccc aagggcgcgc tggcgcggtg ggacctgcgc aacctgccgt
     6121 tcctggacgg ctccttcggc ggcgtgtggg cgcccgcggc gctgcagcac ttcccccgcg
     6181 gcgaaatcct gccggccctg cgcgagctgc ggcgcgtgca ccgcgagggg cccatcttcc
     6241 tgagcttccg ccagggtgcc ggggacctgg aggaggtcga ggacccgccc gcgggcacgg
     6301 tctacgccac caccgtcagc gccgacgagc tgaaggcgct gctgctgggc gtcggctaca
     6361 ccgaggtcga ggtggagcag cggccggacc cgctggagcg ccgtgacgtc acctggctgt
     6421 acggctgggg ccgcctgaag tgatgtcgcc cggcggcgct cagcgctggg cgaaagcgat
     6481 ggcgatcgcg gcggtgttgc ccgcgatcgt gaccgccgcc accgggtgga ccagcgcgcg
     6541 ggcgagcggg ctgctggatg cctgactgga cgggcgcgcc cgcaggacca ggaaccccgt
     6601 gatcgcgagc gcggcggcgg cggccacctc cagggtcgcg gccacgaggg agagcgtgtc
     6661 cgcgccctcc agcagcgagt cgctgagcca ggtgcgccgc ggcgtgaagt gcacgatgag
     6721 gtagcccacg gccagcacca ggccggacca ggcggccgcc agggccgcga gccggtggcg
     6781 ctgcacgcac aggatcacca cggcgatctc caggacgatg gcccccgtgc cgatgacgag
     6841 cacgtccagg cccgccgtgt ccgcgccccg gcgcacgtgg tcgaagttgt gcagcaggat
     6901 caccgcggcg aagaacgtcg ccgccaccat gagggcgctc tccgaagaac gtcctgatgc
     6961 ctgggtggcc ggcgatccca cggtctgcgc catagggcac ccctcccggt caagacagcg
     7021 gtcgtggcgc acagcctaga gccggcctcc gccgcgcacg ccaccttcaa cgctcgttga
     7081 ccggaatggg cagagcttca acgttggacg tagaacgttg caccttgcag ggatggatcc
     7141 aacgaagttt gtcgctcgcc gaccgcgccc tggggcgact cgcctgcgcc ggacggcagt
     7201 tgcccaatcc ccacatactc atcaggccgt acctcgtccg cgaggctgtg gcgagttcga
     7261 ggattgaagg gacgcaggcc tcgttgtctg aggtgttcga cgccgaggcg caggaagtgg
     7321 aggccacagg agacctcctc gaagtcgcca actacatccg ggcgatggag ctgggtctgc
     7381 gcctcgtgca ggacgttccg atctccacgc gcttcatccg ggaacttcac aagaccctga
     7441 tggccggtgt ccgtggccag gaacgctcgc cgggggagat ccgaacgtcc ccgaactgga
     7501 tcggatcccc tgacaaccgc ccagagacag cggtgttcgt ccccccaccg gtggatgaga
     7561 tgcacgccgc gctgtcggac tgggagcgct tcctgcacta cccgccaccg ctgcccgttc
     7621 tcgtccggtg cgccctgctc cattatcagt tcgagaccat ccacccgttc ctggacggca
     7681 acggtcgcat cgggcgcctg ctcatcgtgc tgttcctcgt cgagcaggga gacctccctc
     7741 agccgctgct ctacacgagt gcgtatttcg aggaacacaa agcggactac tacggccgtc
     7801 tgcagggtga gcgcggagag ctggaagagt ggatccagtt cttcttgacg gccgtcgccc
     7861 gacaggcaca agacgccctc cagcgggcgg aacgactggc ggatctgcgg gagggttacc
     7921 ggcaacgcct cgcggtgggc cggagccgcg cgcacgaggt catcgacctg ttgttcgcca
     7981 acccagtgat gaccgcgaag ttcgtcgcgc gtgggctcgg catcacctct cagggcgcgc
     8041 acaacctcct cggccggctg aaggaggcgg gcgtcgtcga ggaattgccg cgcgtcccgg
     8101 gtcgctcgtt gcgctggcag gcgcgcgaaa tcctcgaggc gttgggcggc ttcgcctcct
     8161 aggccgacaa aaacagagga gccgccgggt accggcggct cctcgtgtac tcggcggctc
     8221 agacgaacgc gctggcaaag atcagcgaga cgatcgtcat caccttgatg aggatgttca
     8281 tcgcgggacc ggacgtgtcc ttgaacgggt cacccacggt gtcgccgacg acggccgcct
     8341 tgtgcggctc gctgcccttg ccgccgtggt tgccggcctc gatgtacttc ttggcgttgt
     8401 cccacgcgcc acccgcgttg gccatgaaga tcgccagcgc gaagccgctc accagcgcgc
     8461 cggccaggaa gccgccgagc gcgtcgacgc tgacgaagcc gaccaccagg ggaaccacca
     8521 cggcgagcgc gccgggaatg agcatctcct tgagcgccgc gcccgtggag atgtccacgc
     8581 acttggcgta ctcggggacc acgccctcct tgccctcgcg caggcccggg atctcccgga
     8641 actggcggcg cacctcctcg atcatcttgt tcgcggcgcg gccgacggcg ttgatcgtca
     8701 gcgcggcgaa caggaacggc agcatggcgc ccaggaacag gccggagaac accgccacgt
     8761 cgccgatgtc cagcgagatg ctcccgccgg cggcctggat cgcctgctcg aacgccttga
     8821 ac
//
LOCUS       DATMKC010000015         4398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_163371, whole genome shotgun sequence.
ACCESSION   DATMKC010000015 DATMKC010000000
VERSION     DATMKC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163371"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VNU01_00540"
     CDS             <1..459
                     /locus_tag="VNU01_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWH31136.1"
                     /translation="PLASSSAAFMSGVVESMTTSDMKAALLEANGGERVDVVLEMVGG
                     STFDGSLAALAPFGRLVTYGTASRELPTPVEPRKLVHGTKAVVGFWLAHCFTRQDFLH
                     PQMRELFDLTLAGKLRPIVGATYPLADARLAHESLRSRGSVGKLLLDPTV"
     gene            569..2284
                     /locus_tag="VNU01_00545"
     CDS             569..2284
                     /locus_tag="VNU01_00545"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HWH31137.1"
                     /translation="MASLIAPHGGELVDLVVDAARAAELKEASRTWASWDLTERQLCD
                     LELLANGAFSPLRGFLSRADYEAVRDGMRLADGTLWPMPITLDVTEAFAEGLSAGDQV
                     ALRDAEGVMLAVLHVEDVWRPDLEEEARKVFGTADTRHPAVAHLLQRSNPVYVGGRVE
                     ALQLPQHYDFHELRRTPAQVRELIARHGWERVVAFQTRNPMHRAHVEITRRAAEEVGG
                     GLLIHPVVGLTKPGDVDHYTRVRVYRKLIDRYPDGSAELSLLNLAMRMGGPREAVWHA
                     IIRRNHGVTHFIVGRDHAGPGNDSAGEPFYGPYDAQELMATYAEELGVGMVPFRMMVY
                     IAEQDRYVPDDEVPEGSTVLSISGTQQRELLARGEDIPEWFTYPEVVEELRRTSPPRE
                     KQGFTVFFTGLSGSGKSTVANVLMVKLLERGGRNVTMLDGDLVRKHLSSGLGFSKEDR
                     DTNIRRIGWVAAEIAKNGGVAVCAPIAPYDATRKAVREMTEAQGGGFALVHVATPLEV
                     CEQRDRKGLYAKARAGELKEFTGISDPYEEPDDAELVINTAVVSAEQAADEVLAWFEK
                     QGYFG"
     gene            complement(2399..2797)
                     /locus_tag="VNU01_00550"
     CDS             complement(2399..2797)
                     /locus_tag="VNU01_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31138.1"
                     /translation="MALAAVVLDLLATLPRGIQPPSVRTQVPVEWSRLFAPPVVALLY
                     GARLGVGPLTILPTWLWWGAFLLGAWLGPLHGMAVGMSFAVARAVSTLGVGMWVRERA
                     AERMGRLRAWEHRTAYALLLLAAAGALSAT"
     gene            complement(3126..>4398)
                     /locus_tag="VNU01_00555"
     CDS             complement(3126..>4398)
                     /locus_tag="VNU01_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31139.1"
                     /translation="AQAGRVAVVVAGGGAITMALEEPALARVCGQSGVAPKCADYDCH
                     DTWGWCWYARGCCADGALKKICDCCAPNTPHPVGYCPSGTRVKCIVESCGADPRLQTK
                     IIRRLRSHDPVELSLAVSRTRFGQAGRPVAVLGDAGSSGFAALAAGLTRVADAPVLLS
                     APDTLDGRVGEELARLQTEFVLLVGRFSPVVEVALVAQGRHVTRLNTQADPVPASAEV
                     GTWVRRTSGARTAVVVLPGVGYQAVAQAGALAAAKGWPLLVGDGAASREVLREPEPVH
                     RTLVVSGDPADAARFPGGEAIAAGDAAGLAIALADRMVAEGVSRDTVALTPVGNAAAA
                     AGLAGLAGPMLTYRAGSLDGAWQWLHAHRAGVQRAFVAGTVEEFGDRPYYDLQSLLNE
                     FEAHLLRGQAGEGLPVISQPRSERPIGKARR"
BASE COUNT          622 a         1647 c         1600 g          529 t
ORIGIN      
        1 ccgttggcct ccagcagcgc ggctttcatg tccggcgtgg tcgagtcgat gacgacgtcg
       61 gacatgaaag ccgcgctgct ggaggccaac ggcggcgagc gcgtggacgt cgtcctggag
      121 atggtcggcg gctcgacgtt cgacggcagc ctggccgcgc tggcgccgtt cggccgtctg
      181 gtcacgtacg gcaccgccag ccgcgagctg cccaccccgg tcgagccgcg caagctcgtg
      241 cacggcacca aggccgtcgt cggcttctgg ctggcccact gtttcacgcg ccaggacttc
      301 ctgcacccgc agatgcgcga actgttcgac ctgacgctgg ccgggaaact gcgccccatc
      361 gtgggcgcga cctacccgct ggccgacgcg cggctggccc acgagtcgct gcgctcccgg
      421 gggtcggtcg gcaagctgct gctcgacccc accgtctgaa cggggggttt tcactgcccg
      481 gcgattgaga tcgccgcagg ccacggggct accgtgggtc ctcgcgcggt gcaaacccgc
      541 gtgcctgccg atccctcaag gagtgcccgt ggcttcgctc atcgcgccgc atggcggcga
      601 gctcgtcgat ctggtggtgg acgccgcgcg cgcggcggag ctcaaggagg cgtcccgcac
      661 ttgggcgtcg tgggacctca ccgagcggca gctgtgcgac ctggagctgc tggccaacgg
      721 cgcgttctcg ccgctgcgcg gtttcctgag ccgggcggac tacgaggcgg tgcgcgacgg
      781 catgcgcctg gccgacggca cgctgtggcc gatgccgatc accctggacg tgaccgaggc
      841 gttcgccgag gggctgtccg ccggcgacca ggtggcgctg cgcgacgccg agggcgtcat
      901 gctcgccgtc ctgcacgtgg aggacgtctg gcgtccggac ctggaggagg aggcgcgcaa
      961 ggtcttcggc accgccgaca ccaggcaccc ggcggtggcc cacctgctgc agcggtccaa
     1021 cccggtgtac gtgggcgggc gcgtggaggc gttgcagctg ccccagcact acgacttcca
     1081 cgagctgcgc cggacgccgg cgcaggtccg cgagctgatc gcgcgccacg gctgggagcg
     1141 cgtcgtcgcg ttccagaccc gcaaccccat gcaccgcgcc cacgtggaga tcacccgccg
     1201 ggccgcggag gaggtcggcg gcggcctgct gatccatccc gtggtgggcc tcaccaagcc
     1261 cggtgacgtg gaccactaca cgcgcgtgcg cgtgtaccgc aagctcatcg accgctaccc
     1321 ggacggctcg gcggagctca gcctgctgaa cctggcgatg cgcatgggcg gcccgcgcga
     1381 ggcggtgtgg cacgcgatca tccgccgcaa ccacggggtc acgcacttca tcgtgggccg
     1441 cgaccacgcc ggcccgggca acgactccgc cggcgagccg ttctacggcc cctacgacgc
     1501 gcaggaactc atggcgacct acgcggagga gctcggcgtc ggcatggtgc cgttccgcat
     1561 gatggtctac atcgccgagc aggaccgcta cgtgcccgac gacgaggtgc ccgaggggtc
     1621 cacggtgctg tccatctccg gcacgcagca gcgcgagctg ctggcgcgcg gcgaggacat
     1681 ccccgagtgg ttcacctacc cggaggtcgt ggaggagctg cgccgcactt ccccgccaag
     1741 ggagaagcag ggcttcacgg tgttcttcac gggcctgtcc ggctccggca agtcgaccgt
     1801 cgccaacgtg ctgatggtga agctgctgga gcgcggcggc cgcaacgtga cgatgctgga
     1861 cggcgacctg gtgcgcaagc acctgtcctc cggcctgggc ttctccaagg aggaccggga
     1921 caccaacatc cgccgcatcg gctgggtcgc ggcggagatc gccaagaacg gcggtgtcgc
     1981 ggtgtgcgcc cccatcgcgc cgtacgacgc gacgcgcaag gcggtgcggg agatgaccga
     2041 ggcgcagggc ggcggcttcg ccctggtgca cgtcgccaca ccgctggagg tgtgcgagca
     2101 gcgcgaccgc aagggcctgt acgccaaagc gcgcgccggc gagctcaagg agttcaccgg
     2161 catcagcgac ccgtacgagg agcccgacga cgccgagctg gtgatcaaca ccgcggtcgt
     2221 gtcagccgag caggccgcgg acgaggtgct ggcctggttc gagaagcagg gctacttcgg
     2281 ctgaggggct gctgctcgac ctgcgggctg gtccgggcgc gctgcagcct gcggacgggc
     2341 tccccgcttc gctccaaggg gacccgtcct cggctgcccc gcgccctccc ctgccgctct
     2401 aggtcgcgct cagcgcgccc gcagcagcca agagcaggag cgcgtaggcg gtgcggtgct
     2461 cccaggcgcg gaggcgcccc atgcgctcgg cggcgcgctc gcggacccac atgccgacgc
     2521 ccagcgtgct gaccgcgcgc gccaccgcga acgacatccc gaccgccatg ccgtgcagcg
     2581 ggcccaacca ggcgcccagc aggaaggcgc cccaccacag ccacgtgggc aggatggtca
     2641 gcggcccgac gccgaggcgc gcgccgtaga gcagcgcgac gacgggcggg gcgaacagcc
     2701 gggaccactc gaccggcacc tgcgtgcgca ccgagggcgg ctggatgccc cgtggcagcg
     2761 tggcgaggag gtcgagcacg accgcggcca gggccacgac cgcggcgtcc cgcggcgacg
     2821 ggcgcggcaa gacgaggccg ggcagcccgg cgaagaagcc gagcagtgcc gccgagccgg
     2881 acgccgcgag cgcgaagacc ccgacgacgg tgagacgctg gcgcccccgg accgggggca
     2941 ggagggtctc ggcgaccgag aggcctcagg gcgaccacag cgaccgcagg ccagcggcga
     3001 aggccagcag gagcgcgatc ccgagcggtt ccacaggatt cacgtccgtg cgggtgcgat
     3061 tcgggcgaca tggcgcccag aacgcaccca tccagggggg gtaccgggct ccgcccggtc
     3121 cgacgtcatc gccgcgcctt gccgatcggg cgctcggagc gcggctgcga gatgaccggc
     3181 aggccctcgc ccgcctggcc gcgcagcagg tgcgcctcga actcgttcaa cagcgactgc
     3241 aggtcgtagt acggccggtc gccgaactcc tccacggttc cggccacgaa cgcgcgctgc
     3301 acccccgccc ggtgcgcgtg cagccactgc cacgcgccgt ccagcgaccc tgcgcggtac
     3361 gtcagcatgg ggccggccag gccggccagc cccgcggccg cggcggcgtt gcccaccggg
     3421 gtcagcgcga ccgtgtcccg cgagacgcct tcggccacca tgcggtcggc cagcgcgata
     3481 gcgagccccg cggcgtcgcc cgccgcgatc gcctcaccgc cggggaagcg cgccgcgtcg
     3541 gccgggtcgc cggagaccac gagcgtgcgg tggaccggct ccggctcccg cagcacctcg
     3601 cgcgaggcgg cgccgtcgcc caccaggagc ggccagccct tggcggccgc gagtgcgccc
     3661 gcctgcgcca ccgcctggta gcccacgccc ggcagcacca ccacggcggt gcgcgcgccc
     3721 gaggtgcgcc gcacccaggt gcccacctcg gccgaggccg ggaccgggtc ggcctgcgtg
     3781 ttgaggcggg tgacgtgccg gccctgcgcc accagcgcga cctccaccac ggggctgaaa
     3841 cgccccacga gcagaacgaa ctccgtctgc agccgggcga gctcctcgcc gactctgccg
     3901 tccagcgtgt caggtgccga cagcagcacc ggcgcgtccg ccacgcgcgt gagcccggcc
     3961 gcgagcgccg cgaagccgct gctcccggcg tccccgagca cggccaccgg ccgccccgcc
     4021 tggccgaacc gcgtccggct caccgccagc gacagctcca ccggatcgtg ggaacgcagc
     4081 cgccggatga tcttggtctg cagccggggg tcggccccgc agctctccac gatgcacttc
     4141 acgcgcgtcc cgctggggca gtagcccacg gggtgcggcg tgttcggcgc gcagcagtca
     4201 cagatcttct tgagcgcgcc gtcggcgcag cagccgcgcg cgtaccagca ccagccccag
     4261 gtgtcgtggc agtcgtagtc ggcgcacttg ggcgccacgc cggactgccc gcacacgcgg
     4321 gcgagcgccg gctcctccag cgccatggtg atggcgccgc cgccggccac cacgaccgcc
     4381 acgcggccgg cctgcgcc
//
LOCUS       DATMKC010000016         4693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_163227, whole genome shotgun sequence.
ACCESSION   DATMKC010000016 DATMKC010000000
VERSION     DATMKC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163227"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..1005
                     /locus_tag="VNU01_00560"
     CDS             412..1005
                     /locus_tag="VNU01_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWH31140.1"
                     /translation="MLVPFPEEVADGVRAGDPDAVGAVYVALSDRLLGYLMARVRDRG
                     VAEDLLEATFLELLQKGRTIRGGAASIKVWLFRAAYFNALDHLRKVKRRMEDLTDDEE
                     ALDIVDHGVSSPEDSAIRADVRRRVRQAMTQLSPEQRQVLLLRYVAGLSAPEVGAVLD
                     KSDGAIRSLQHRAERALAKLLESERESSAPISGPGTS"
     gene            1160..2134
                     /locus_tag="VNU01_00565"
     CDS             1160..2134
                     /locus_tag="VNU01_00565"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HWH31141.1"
                     /translation="MSKHSPHRDVDPALGALIEAAYTERVDMDVAARHLWVIHRAAGA
                     VRGQGRRTRKALVSVLAGGMLFMSSGGAVAASADSLPGDMLYTVKRGVEAARIVLTVS
                     SEGDARLHLALAQRRMAELRAAAAKRPEAVPALVRDAQVSLASAERTGGTEVAHEVQE
                     ASAQLTVLVAVIDESAGDRVASRKTTTAESEQAMMASASPGPSEQGASASPSASPSDG
                     QMALAGEPSEGQSASASPSPSQSASATPSASATPSEPSESATPTPTPTETATESASPT
                     PTATTPPSSEPAPTSSPGRKYESVEPTESPTGTQTPTPSPTPEETPTG"
     gene            2181..2555
                     /locus_tag="VNU01_00570"
     CDS             2181..2555
                     /locus_tag="VNU01_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31142.1"
                     /translation="MKGIDVGLSARTYWVWKRVCHGLGLHWERLTTSVEDPRSIWAWK
                     QEVVVERFHASILRRLAEKALAAGEPMGQGQDWRTAGGDEAEWLELDDLLRTLRAHRE
                     IEDIRLLARAEPPEDELVDERE"
     gene            2603..3238
                     /locus_tag="VNU01_00575"
     CDS             2603..3238
                     /locus_tag="VNU01_00575"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HWH31143.1"
                     /translation="MPTTTEALIIALLFLAPGLLYDYGVTRSAPYRRPDTGERLWRAF
                     ANSLIFQAAALPLTFLVWRHYAWMDLSHAPPASLAVLGLVAGRYVAVPYGAGTLRGRR
                     VRSRISETPSGWDHAFRHLAGGGYVRVKLKTGEWIAGLYAHLDGQVPPYASGYPNDPT
                     AYLPAQLEVDPFTGAILLDGAAGARVREWGVLLRAEECERVEFQPLPRRAS"
     gene            3235..3462
                     /locus_tag="VNU01_00580"
     CDS             3235..3462
                     /locus_tag="VNU01_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31144.1"
                     /translation="MTDMTKTTRNEEWGPYTSTRRVDIRALPPVRDAGGISGGLEDVL
                     RHIPERELERVLERFARRTRGARRLRSDDED"
     gene            complement(3459..>4693)
                     /locus_tag="VNU01_00585"
     CDS             complement(3459..>4693)
                     /locus_tag="VNU01_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HWH31145.1"
                     /translation="KNLVDIILTNNGYEVRNLGIKQPIDAIVAAAEDFDADAIGLSGL
                     LVKSTVVMREDLEELVRRGLTRWPVLLGGAALTRNYVEADLRKVYPGPVHYCRDAFAG
                     LRTMDELAELKKAGGIPDDWGRLVKELPLARGRRDDRPSVDEVVQTDVRRDVAVPEPP
                     FWGSRVVRGLSIEEILPFLNKTALFRNQWGLRNADFDKGEEALRHILEMAVRERLLVP
                     EVVYGYWQANADGNELIVWAAPDGDEVAATFSFPRQRTGRRLSIPDFFRPRSSGERDV
                     IAFQAVTVGQRVSERAQELFAADRYTDYLYLHGLGVEMAEALAEYWHKRVRTELGIAG
                     DDAEDMESLFDQGYQGSRYSFGYAACPDLEARKPLFELLQAQRIGLELSEEFQLVPEQ
                     STDAFVVHHPEAKYFNAR"
BASE COUNT          684 a         1627 c         1678 g          704 t
ORIGIN      
        1 cgcgcaggcg ctccaactcg tcgctggacg aatcgagctt tgcctgcagt ttggagattt
       61 tgtcctcgtc gcgcagcgcg cgcggatcgc gcagcgccat gagatagttg cggacggccg
      121 agtcggcggc ggaagaggcc attgcgggat gcctttcttg atgtggggac gtttgcgcgc
      181 agcatattcg ttttgcacgc gcgatggcga atgcttgttg ccgcccggcc gacgctcccg
      241 agcggccggc cgcccggccc aagcccgcgc gactctttgc ggtttcgcta cggcgcgcga
      301 ggggggtgct ctgtcatact tcaccgcaac tgtcctgtcg gcctcgtccc gggccgccgc
      361 cgcattgccg cccgggccgc cacatgttgt tcaccgctga accgattgga cgtgctggtg
      421 cccttccctg aagaggtggc ggacggcgta cgcgcggggg atcccgacgc ggtcggcgcc
      481 gtctacgtcg cgctgtccga tcgcctgctc ggctatctga tggcgagggt gcgcgaccgc
      541 ggcgtggcgg aggacctgct ggaggccacg ttcctcgagt tgctgcagaa gggccgcacg
      601 atcaggggcg gcgccgcctc catcaaggtg tggctgttcc gggccgcgta cttcaatgcc
      661 ctcgaccacc tgcgcaaggt caagcggcgg atggaggacc tgaccgacga cgaggaggcg
      721 ctggacatcg tcgaccacgg cgtgtccagc cccgaggact ccgccattcg agccgacgtc
      781 cggcggcggg tccgccaggc catgacccag ctgtccccgg agcagcgcca ggtgctgctg
      841 ctgcgctatg tcgcgggact ttcggcccct gaagtcggag cagttctgga caaatcggac
      901 ggcgcgatcc ggagcctcca gcaccgggcg gagcgcgcct tggcgaaact gctcgaatcc
      961 gagcgggagt cgtccgcacc gatcagcggc cccggcacgt cctaggagcg aggctcggcg
     1021 ccgcgggctt gtttctctgc ggcttcggga ctcccggaac cgcgagtttg gccgcacaat
     1081 gtcatggtgg ccggccgcta cgagcccggt accgcgaagg aacgcccgag cccgcccaac
     1141 cgacgtctgt catgagccga tgagcaagca cagtccccac cgcgatgtcg atccagcgct
     1201 cggtgcgctg atcgaagccg cgtacaccga gcgcgtggac atggatgtgg cggcacgcca
     1261 cctctgggtc atccaccgcg ccgcgggggc ggtgcgtggc cagggccggc gcacgcgcaa
     1321 ggcgctggtg tccgtgctgg cgggcggcat gctgttcatg tcctccggcg gcgcggtggc
     1381 cgcgagcgcc gattcgctgc ccggggacat gctctacacc gtcaagcgag gggtcgaggc
     1441 ggcgcgcatc gtgttgacgg tgagctccga gggcgacgcg cggctgcacc tggcgctggc
     1501 gcagcgtcgc atggccgagc tgcgcgccgc ggcggccaag cgtccggagg cggtgccggc
     1561 gctggtgcgc gacgcacagg tctcgctggc ctccgccgag cggacgggcg gcaccgaggt
     1621 ggcccacgag gtgcaggagg ccagcgcgca gctgaccgtg ctggtcgccg tcatcgacga
     1681 gtcggcgggc gaccgcgtcg ccagccgcaa gaccaccacc gcggagtccg agcaggcgat
     1741 gatggcctcc gcctcgccgg ggccgtcgga gcagggcgcc tccgcgtcgc cgagcgcgtc
     1801 gcccagcgat gggcagatgg ccctcgccgg cgagccgtcc gagggccaga gcgcgtccgc
     1861 gtcgccgtcg cccagccagt cggccagcgc aaccccgagc gcctccgcca cgccgagcga
     1921 gccgtcggag tccgcgacgc cgacgccgac gccgaccgag accgccaccg agagcgccag
     1981 cccgaccccg acggcgacga ccccgccgtc ctcggagccg gcgccgacgt cgagccccgg
     2041 ccgcaagtac gagtcggtgg agccgacgga gtcgccgacc gggacgcaga cgccgacgcc
     2101 ctcgcccacg ccggaggaga ccccgaccgg ctgagccttc ctgccgcacg gccccgccgc
     2161 cagcaagtag ggtttggctc gtgaagggca tcgatgtcgg cctgtcggct cgcacgtact
     2221 gggtgtggaa gcgcgtctgt cacggcctcg gcctgcactg ggagcgcctg accacttcgg
     2281 tggaggaccc gcgctccatc tgggcgtgga agcaggaagt cgtggtggag cgcttccacg
     2341 cgtccatcct gcggcggctg gcggagaagg cgctggcggc gggcgagccc atgggccagg
     2401 gccaggactg gcggacggcc ggcggcgacg aggccgagtg gctggagctg gacgacctgc
     2461 tgcgcacgct gcgcgcccac cgcgagatcg aggacatccg cctgctcgcc cgcgccgagc
     2521 cccccgagga cgaacttgtg gacgagcgag agtgacggac ggctcggccg catagccttg
     2581 acggcatcac acgggggcga cgatgccaac caccacagaa gcactcatca ttgcgctgct
     2641 gttcctggcg ccggggttgc tctacgacta tggggtaacg cgcagtgcac cgtatcgtcg
     2701 gcccgacacc ggcgagcggt tgtggcgcgc gttcgccaac tcgctgatct tccaggctgc
     2761 ggcgcttccg ctcacgttcc tggtttggcg gcactacgca tggatggacc tgtcgcacgc
     2821 tccgcccgcc tccctcgccg tgctggggct ggtcgccggg agatacgtcg ccgtgcccta
     2881 cggagcggga acactgcgtg gacggcgcgt tcgctcgcgg atatcggaga cgccctccgg
     2941 ctgggatcac gcgtttcgtc accttgcggg gggcggctac gtccgagtga agctgaagac
     3001 cggtgaatgg atcgccggtc tctatgcgca cctcgatggg caggtccccc cgtacgcgtc
     3061 tggatatccc aacgacccca ccgcatacct gcccgcccaa ctcgaagtcg atcctttcac
     3121 cggcgccatt ctccttgatg gtgcagcagg ggctcgggtg agagagtggg gggttcttct
     3181 ccgggccgag gaatgtgagc gggttgagtt ccaaccactg ccgaggagag catcatgacg
     3241 gacatgacca agacgacgcg caacgaggag tgggggccgt acacctcgac ccggcgcgtc
     3301 gacatccgtg ctttgccacc agtacgtgac gcagggggga tctcgggagg ccttgaggac
     3361 gtgctgcgtc acatcccaga acgggaactg gaacgggtcc tggaacgctt cgcgcgtcgg
     3421 acccgggggg cgaggcggct gcggtccgac gacgaggatt agcgggcgtt gaagtacttg
     3481 gcctcggggt ggtggaccac gaacgcgtcg gtggactgct cggggacgag ctggaactcc
     3541 tcggagagct ccaggccgat gcgctgcgcc tggagcagct cgaacagcgg cttgcgggcc
     3601 tccaggtccg ggcaggcggc gtagccgaag ctgtagcgcg agccctggta gccctggtcg
     3661 aacagggact ccatgtcctc cgcgtcgtcg ccggcgatgc cgagctcggt gcgcacgcgc
     3721 ttgtgccagt actccgccag cgcctcggcc atctccacgc cgaggccgtg caggtacagg
     3781 tagtcggtgt agcggtcggc ggcgaagagc tcctgcgcgc gctcgctcac ccgctgcccg
     3841 acggtgaccg cctggaacgc gatcacgtcg cgctcgccgg atgagcgcgg gcggaagaag
     3901 tccgggatgg acaggcgccg cccggtgcgc tgccggggga agctgaacgt cgcggcgacc
     3961 tcgtcgccgt ccggcgccgc ccacacgatc agctcgttgc cgtcggcgtt ggcctgccag
     4021 tagccgtaca cgacctccgg caccagcagc cgctcgcgca ccgccatctc caggatgtgg
     4081 cgcagcgcct cctcgccctt gtcgaagtcg gcgttgcgca ggccccactg gttgcggaac
     4141 agcgccgtct tgttcaggaa cggcaggatc tcctcgatgg acagcccgcg caccacgcgg
     4201 ctgccccaga acggcggctc cggtacggcc acgtccctgc gcacgtcggt ttgcaccacc
     4261 tcgtcgacgg acggacggtc gtcgcggcgc cccctggcca gcggcagctc cttgaccagg
     4321 cggccccagt cgtcggggat gccaccggcc ttcttgagct cggcgagctc gtccatcgtg
     4381 cgcaggccgg cgaacgcgtc gcggcagtag tgcacgggcc cgggatagac cttgcgcagg
     4441 tccgcctcga cgtagttgcg cgtcagcgcg gccccgccca gcagcaccgg ccagcgcgtc
     4501 aggccgcggc gcaccagctc ctccaggtcc tcgcgcatca ccacggtgga cttcaccagc
     4561 aggccggaca ggccgatcgc gtcggcgtcg aagtcctcgg cggcggccac gatcgcgtcg
     4621 atcggctgct tgatgcccag gttgcgcacc tcgtagccgt tgttggtcag gatgatgtcg
     4681 acgaggttct tgc
//
LOCUS       DATMKC010000017         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_210885, whole genome shotgun sequence.
ACCESSION   DATMKC010000017 DATMKC010000000
VERSION     DATMKC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210885"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /gene="rny"
                     /locus_tag="VNU01_00590"
     CDS             <1..1160
                     /gene="rny"
                     /locus_tag="VNU01_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWH31146.1"
                     /translation="LAERHTQALERAAGLTAEQARRELLAGEEEAVRLRVRRLAADLE
                     AEAREHAEARARDLIATAVQRLALSTVSPATTVAVPLPEGDMKARIIGKEGRNIRTFE
                     ERTGVNVVIDELPDAVLLSSFDPRRREVARVALRALIADGRIYPTTIEEQVALAEEHV
                     SVETERAAIDAATDARVYDLSPELVESLGELYVRTSYGQNVLQHSVEVAHLAGMMAQE
                     LGVDADLARRAGLLHDIGKARGQDEPGSHAAVGARLAARIGEPAEVCHAIEAHHGEVE
                     PATPEAVLVQVADQISLSRPGGPREPLEQHMSRMRAIEELCRSFDGVERVHAMQAGRD
                     IRVMVSPKRVGDEEARELARTIAARVESELAFPGRVAVTVVRELRATEFVR"
     gene            1227..1520
                     /locus_tag="VNU01_00595"
     CDS             1227..1520
                     /locus_tag="VNU01_00595"
                     /inference="COORDINATES: protein motif:HMM:NF016147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HWH31147.1"
                     /translation="MSAFRVSSTSSANAVGWAVAATIRGEGSAELHVVGAGAANQALK
                     AIAIARALLRDDDVDLVCAPEFMDVEIEGLVRTALRLVVDGRDQRDAQGAPSE"
     gene            1540..2940
                     /gene="miaB"
                     /locus_tag="VNU01_00600"
     CDS             1540..2940
                     /gene="miaB"
                     /locus_tag="VNU01_00600"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWH31148.1"
                     /translation="MTARTYFIRTFGCQMNEHDSERAAGVLEIMGYRAAGDAESADLV
                     LFNTCAIRENADNKLYGQLSALKPLKLADPAKTVVVGGCLAQKDQGELARRAPWVDVV
                     YGTHNMGSLPKLLAQADSSALPVIEILEHTEMFPSALPARREVGHHAWVSISIGCDNT
                     CTFCIVPSLRGPEKSRRMGDILGEVRALVADGVREVSLLGQNVNSYGRDLVRAGEVAE
                     PGPRNRTLFAELLHGLGDVDGLERVRFTSPHPKDFTSDVFAAMRDVPNVCEQLHLPLQ
                     SGSDRVLKLMQRSYRSSRYLELVAEARETIPGVAISTDIIVGFPGETEEDFAETLRVA
                     EAAAFDSAYTFEYSKRPGTAAVDMDGHLDAGVVNARYRRLAALTDELALRSNERQLGT
                     VQELLIEGLSKRDAAMASGRTRTNRLVHVPVGPGVEAGALVRARLDRAAPHYLVGALT
                     DAPCDVPVGAPLPVRS"
BASE COUNT          414 a         1052 c         1103 g          373 t
ORIGIN      
        1 gcctcgccga gcggcatacc caggcgctgg agcgcgccgc cgggctgacc gccgagcagg
       61 cgcgtcgcga actgctcgcc ggcgaggagg aggcggtgcg gctccgggtc cgccgcctcg
      121 ccgcggacct ggaggcagag gccagggagc acgccgaggc gcgggcccgc gacctgatcg
      181 ccacggccgt ccagcgcctg gcgctgtcca ccgtcagccc ggccaccacc gtggcggtgc
      241 cgttgcccga gggcgacatg aaggcccgca tcatcggcaa ggagggccgc aacatccgca
      301 ccttcgagga gcggaccggc gtgaacgtcg tcatcgacga gctgccggac gccgtgctgc
      361 tgtccagctt cgacccgcgt cgccgggagg tcgcccgggt ggcgctgcgc gcgctgatcg
      421 cggacgggcg catctacccc accaccatcg aggagcaggt cgcactggcc gaggagcacg
      481 tcagtgtgga gacggagcgc gccgccatcg acgccgccac ggacgcgcgg gtctacgacc
      541 tgtcgcccga gctcgtcgag tcgctcggcg agctgtacgt gcgcacctcc tacgggcaga
      601 acgtgctgca gcactccgtg gaggtggcgc acctcgcggg gatgatggcc caggagctgg
      661 gtgtggacgc cgatctggcc cggcgcgccg ggctgctgca cgacatcggc aaggcgcgcg
      721 gccaggacga gcccggcagc cacgccgccg tcggcgcgcg gctggccgcg cgcatcggcg
      781 agcccgccga ggtctgccac gccatcgagg cgcaccacgg cgaggtggag cccgccacgc
      841 cggaggcggt cctggtgcag gtcgccgacc agatctccct gtcccggccg ggcggcccgc
      901 gggagccgct ggagcagcac atgagccgca tgcgcgccat cgaggagctg tgccggtcct
      961 tcgacggggt ggaacgggtg cacgcgatgc aggccggccg ggacatccgc gtgatggtga
     1021 gcccgaagcg ggtgggcgac gaggaggcgc gcgagctggc gcgcaccatc gccgcccgcg
     1081 tggagagcga gctcgccttc ccggggcggg tcgccgtcac cgtggtgcgg gagctgcgcg
     1141 cgacggagtt cgtgcgctga cggatggatc ggacacgcac cgcggagctg gggggcgcca
     1201 gacgagccgg aaagggatga cgtcccatca gcgccttcag ggtttccagc acatcgagcg
     1261 cgaacgccgt cgggtgggcc gtcgccgcga cgatccgcgg cgagggctcg gccgagctgc
     1321 acgtggtggg cgcgggagcg gccaaccagg cgctgaaggc catcgccatt gcgcgcgcgc
     1381 tgctgcgcga cgacgacgtc gacctggtgt gcgcgccgga gttcatggac gtcgagatcg
     1441 agggcctggt gcgcacggcg ctgcggctgg tggtcgacgg ccgggaccag cgcgacgccc
     1501 agggtgcccc gtccgagtag gccgcttatc cttggcgccg tgaccgcacg cacctatttc
     1561 atccgcacct tcgggtgcca gatgaacgag cacgactccg agcgcgccgc cggcgtgctg
     1621 gagatcatgg gctaccgcgc cgccggcgac gccgagtcgg ccgacctcgt gctgttcaac
     1681 acgtgcgcga tccgcgagaa cgccgacaac aagctctacg ggcagctgtc ggcgctcaag
     1741 ccgctgaagc tcgccgaccc cgccaagacg gtggtggtcg gcggctgcct ggcgcagaag
     1801 gaccagggcg agctggcgcg ccgcgctccg tgggtggacg tggtctacgg cacgcacaac
     1861 atgggctcgc tgcccaagct tttggcgcag gccgactcca gcgccctgcc ggtgatcgag
     1921 atcctggagc acaccgagat gttcccctcg gcgctgccgg cgcggcgcga ggtcggacac
     1981 cacgcctggg tgtccatctc catcggctgc gacaacacct gcacgttctg catcgtgccg
     2041 agcctgcgcg ggccggagaa gtcccggcgc atgggcgaca tcctgggcga ggtccgcgcg
     2101 ctggtcgccg acggcgtgcg cgaggtgtcg ctgctgggcc agaacgtcaa ctcctacggc
     2161 cgcgacctcg tgcgcgccgg cgaggtggcc gagcccgggc cgcgcaaccg cacgctgttc
     2221 gccgagctgc tgcacgggct gggcgacgtg gacggcctgg agcgcgtgcg cttcaccagc
     2281 ccgcacccca aggacttcac ctccgacgtg ttcgccgcca tgcgcgacgt gccgaacgtg
     2341 tgcgagcagc tgcacctgcc gctgcagtcc ggcagcgacc gggtcctcaa gctcatgcag
     2401 cgcagctacc gctcctcgcg ctacctggag ctggtcgccg aggcgcgcga gaccatcccc
     2461 ggcgtggcga tctccaccga catcatcgtg ggtttccccg gcgagaccga ggaggatttc
     2521 gccgagacgc tgcgcgtcgc cgaggccgcc gccttcgaca gcgcgtacac gttcgagtac
     2581 tccaagcgtc ccggcacggc ggccgtggac atggacgggc acctggacgc cggcgtggtc
     2641 aacgcgcgct accggcggct ggcggcgctg accgacgagc tggcgctgcg gtccaacgag
     2701 cggcagctcg gcacggtcca ggagctgctg atcgagggcc tgtccaagcg cgacgcggcg
     2761 atggccagcg gccgcacgcg cacgaaccgc ctcgtgcacg tcccggtggg gcccggcgtg
     2821 gaggcggggg cgctggtgcg cgcgcggctg gaccgcgccg ccccccacta cctggtgggt
     2881 gcgctcacgg acgcgccctg cgacgtgccg gtcggcgcgc ccctgcccgt ccggtcttga
     2941 ac
//
LOCUS       DATMKC010000018         6693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_210805, whole genome shotgun sequence.
ACCESSION   DATMKC010000018 DATMKC010000000
VERSION     DATMKC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210805"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VNU01_00605"
     CDS             complement(<1..416)
                     /locus_tag="VNU01_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWH31149.1"
                     /translation="MGAVETLQGRVGQELGRSGWLMVDQERVDRHADNTGDAQWIHND
                     PARAAAEGPFGGPIAQGFLLLSLFTALAEQAMPQADGLEYLVNYGFDRVRFIRPVRVG
                     DAVRLRVRLADVSQNPGGRWIVALDAELESREAADAA"
     gene            573..2585
                     /gene="tkt"
                     /locus_tag="VNU01_00610"
     CDS             573..2585
                     /gene="tkt"
                     /locus_tag="VNU01_00610"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWH31150.1"
                     /translation="MSDLDLLAVNTVRTLSMDAVQAANSGHPGTPMALAPLGYSLFTR
                     WLAHNPADPSWPDRDRFVLSAGHASMLLYSLLHLTGYDLPLEDLRNFRQLGHRTPGHP
                     ENFVTRGVETTTGPLGQGVGNAVGMALAERILAERFNRPGHDIVDHRTVVIASDGDLM
                     EGVSAEASSLAGHLGLEKLVVYWDDNSITIDGRTDLSFTGEDVCARYAAYGWRVLAVD
                     DVNDLDELDRVTKEALTPDGRPTFVRVKTVIGYGSPNKADTSAAHGAPLGAEEIAATK
                     RNLGWDYPEPFTVPQEAARHADQRERGADLQEDWERRFNAYAVDHPELAAEFRRVMAG
                     ELPEGWDADLPKWETGAKEATRKSSGAVLNALAARLPELVGGSADLAESNNTRISDGG
                     DVLAGAYGHRNVNYGVREHAMGSVMNGMVLHGGIRPFGGTFLIFTDYLRPSLRLACLM
                     GLPVVYVMTHDSIGLGEDGPTHQPVEHLAALRAIPNLHVMRPADARETAGAWRHAIAR
                     TDGPTLLALTRQGVPVLEGSAAEEVARGAYVVVPDDDNPDVVLIATGSEVSLAVEAAA
                     ILAEHDISARVVSMPCWELFEQQGGAYQDLVLPPDVPALSIEAATSFGWSRWADEHIA
                     LDRFGASAPAEALFEHFGFTPQVVAQAAEELLDSFAGDYDDEEQHA"
     gene            2582..3655
                     /gene="tal"
                     /locus_tag="VNU01_00615"
     CDS             2582..3655
                     /gene="tal"
                     /locus_tag="VNU01_00615"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWH31151.1"
                     /translation="MNPLQRLRDLGQSAWLDSIQRSYLEPGGYLDTLIAAGEIDGLTS
                     NPSIFEQALGKDRSYDAQLAELKGSDPREALWELMKADITVACDKFLPMWRASGGAHG
                     YVSIEVDPSKAFDTAATTDEGSKLFAELDRPNLMIKVPGTEPGLEAVSRLLAEGVNVN
                     VTLLFSVARYEAVIAAYLDGLRRRADRGGDLRAVASVASFFVSRVDSKVDALLGPDHP
                     RLATAAIANARVAYGSFVREFSGPGFADLRELGARVQRPLWASTSTKNPGYRDVLYVE
                     ELAGPDTVNTMPEVTLDAFRDHGVVEDRIGGSWDEAAHQLERLAEEGVDLDQITKELE
                     AEGVEKFVQSFDAAVDAVAAHVS"
     gene            complement(3652..4233)
                     /locus_tag="VNU01_00620"
     CDS             complement(3652..4233)
                     /locus_tag="VNU01_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWH31152.1"
                     /translation="MDFSEVVRRRRMVRAYRPDPVAPDALRRMLDVARRAPSAGFTQG
                     QSFVVLTAQEARARMAELCGEADYVARGFDAWVSTAPVHVVPCVSRAAYEERYAEPDK
                     APSTKPHEWAVPYWWVDGGAALMLLLLAAVDEGLAAGVLALDDTPPLRELLGLRPEEV
                     PLGLVTVGYPAPDRPSGSLARGRKDYSEQVRFR"
     gene            4361..4804
                     /locus_tag="VNU01_00625"
     CDS             4361..4804
                     /locus_tag="VNU01_00625"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH31153.1"
                     /translation="MHTFTESIHIERPRQQVWRYLVDPELAKAWVGFEQMDLIGDQPL
                     AKGTRIQEVFRFLGKRMSMVDEVVEYQDGAHIAFRSVEAPIANEVDETLTDEDGGTRV
                     ELVMRTDDTQGFFGKLAEPVIERMFARELRQQLHRLKDLCEADAN"
     gene            complement(4820..>6693)
                     /locus_tag="VNU01_00630"
     CDS             complement(4820..>6693)
                     /locus_tag="VNU01_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWH31154.1"
                     /translation="LAEVIDACLALIRKPSATADELMKHVPAPDFPTGARIVGGDGIR
                     DAYLTGRGAVTMEAVATTETRSGGLPRIVVTEIPFQVNKAALLERMADLVKDKKLDEI
                     RDLRDESSREGMRVVIELKRGEDPARVLRKLYRLTDLRTNFNVNLVALVDGQPRTIGL
                     REALTEYLAHQRTVLTRRTTYRKEKAEARAHVLEGLLVALDNLDAVIKLIRAAESADK
                     AREQLMKRFSLTEVQATAILDMQLRRLAKLERDKVKAEHAELTKVITELKAILADPAK
                     LDALLAAELKEIRARHANPRRSRLVVTADDAPDDDAGTPGARTAGTRGAELIVDEDLP
                     ELEAQPVTLYVTQGGYLKAVPRRRVSKPHSHPNDPVVAVLRATTEDTVLLIDADGAGY
                     RIGVGDVPVTTMRQRGTSIAQLLGDGPDARIAGAVLLGPDVETVLTVSARGLVKRTER
                     AEYEGRLRTMIAAGVRDDDELVAVAPCNDGDHIVLANSAGLVVRFAAGEVRPMGRGAA
                     GVAGMNVPRGARIVSATVVPGGGGEDTPVLTVDADGAAKRTALGEYPAKGRGGKGVQT
                     GAARLAFCGVGGDLHAGTAGGAVVLETAELPAGKRPGRLAPITGHVTGPVVAETGRD"
BASE COUNT          898 a         2476 c         2414 g          905 t
ORIGIN      
        1 gcggcgtcgg cggcttcccg ggactccagt tccgcgtcca gggccacgat ccagcgcccg
       61 ccggggttct ggctcacgtc ggccagccgg acgcgcagcc gcaccgcgtc gcccacccgc
      121 accggccgga tgaagcgcac ccggtcgaag ccgtagttga ccaggtattc cagcccgtcg
      181 gcctgcggca tggcctgctc ggccagcgcg gtgaacagcg acagcagcag gaagccctgc
      241 gcgatcggcc cgccgaacgg gccctccgcc gccgccctgg ccgggtcgtt gtggatccac
      301 tgcgcgtccc cggtgttgtc ggcgtggcgg tccacccgct cctggtcgac catcagccag
      361 cccgagcgcc ccagctcctg cccgacccgc ccctgcagcg tctccaccgc gcccaccgtg
      421 gcctcctcga ggtgtcgccg gaggtcccgc acggcccggc accgtcggca cgcggcggtt
      481 gcgaccctcc gcggctgccg cgcatcgtag gctagggcgc cgccggcgat gcggcgtcct
      541 cgtttccgcg cgacaggagt tccgggccca agatgagcga tctcgacctg cttgcggtca
      601 acaccgtgcg caccctttcc atggacgcgg tgcaggccgc gaactccggg caccccggca
      661 cgccgatggc gctggcgccg ctgggctact cgctgttcac gcgctggctg gcgcacaacc
      721 cggcggaccc gtcgtggccg gaccgggacc gcttcgtgct gtcggccggc cacgcgtcga
      781 tgctgctgta ctcgctgctg cacctcaccg gctacgacct gccgctggag gacctgcgca
      841 acttccgcca gctcggccac cgcacgccgg gccacccgga gaacttcgtc acgcgcggcg
      901 tggagaccac caccgggccg ctcgggcagg gcgtgggcaa cgcggtgggc atggcgctgg
      961 ccgagcgcat cctggcggag cgcttcaacc ggccgggtca cgacatcgtc gaccaccgca
     1021 ccgtggtgat cgccagcgat ggcgacctca tggagggcgt gtcggcggag gcctccagcc
     1081 tggcgggcca cctgggcctg gagaagctgg tcgtctactg ggacgacaac tccatcacca
     1141 tcgacggccg caccgacctg tcgttcacgg gcgaggacgt gtgcgcgcgc tacgccgcct
     1201 acggctggcg cgtgctggcg gtcgacgacg tcaacgacct ggacgagctg gaccgggtca
     1261 ccaaggaggc gctcacgccg gacggccgcc ccaccttcgt gcgcgtcaag acggtcatcg
     1321 gctacggctc gccgaacaag gccgacacct ccgccgcgca cggcgcgccg ctgggcgccg
     1381 aggagatcgc ggccaccaag cgcaacctcg gctgggacta cccggagccg ttcacggtcc
     1441 ctcaggaggc ggcccgccac gccgaccagc gcgagcgcgg cgccgacctg caggaggact
     1501 gggagcgccg cttcaacgcc tacgcggtgg accaccccga gctggccgcc gagttccgcc
     1561 gcgtgatggc cggtgagctg ccggagggct gggacgccga cctgccgaag tgggagacgg
     1621 gcgccaagga ggccacccgc aagtccagcg gcgcggtgct caacgcgctc gcggcacggc
     1681 tgccggagct ggtcggcggg tccgcggacc tggcggagtc caacaacacg cggatctccg
     1741 acggcggcga cgtcctggcg ggcgcgtacg ggcaccgcaa cgtcaactac ggggtgcggg
     1801 agcacgccat gggctcggtc atgaacggca tggtcctgca cggcggcatc cggccgttcg
     1861 gcggcacgtt cctgatcttc accgactacc tgcgcccgtc gctgcggctg gcctgcctca
     1921 tgggcctgcc ggtcgtctac gtgatgacgc acgactccat cggcctgggc gaggacggcc
     1981 cgacgcacca gccggtggag cacctggccg ccctgcgcgc gatcccgaac ctgcacgtga
     2041 tgcggccggc ggacgcgcgc gagaccgcgg gcgcctggcg ccacgcgatc gcgcggaccg
     2101 acggcccgac gctgctggcg ctcacccgcc agggcgtgcc cgtgctggag ggcagcgcgg
     2161 ccgaggaggt cgccaggggc gcgtacgtgg tggtgcccga cgacgacaac cccgacgtgg
     2221 tgctgatcgc cacgggcagc gaggtctccc tggccgtgga ggcggcggcg atccttgccg
     2281 agcacgacat ctccgcgcgt gtggtctcca tgccgtgctg ggaactgttc gagcagcagg
     2341 gcggcgccta ccaggacctg gtgctgccgc cggacgtgcc ggcgctgtcg atcgaggcgg
     2401 ccaccagctt cggctggtca cggtgggccg acgagcacat cgcgctggac cgcttcggcg
     2461 cctccgcccc cgccgaggcg ctgttcgagc acttcggctt cacgccgcag gtggtggcgc
     2521 aggcggccga ggagctgctg gacagcttcg ccggcgacta cgacgacgag gagcagcacg
     2581 cgtgaacccc ctccagcgcc tccgcgacct cggccagtcc gcctggctgg actccatcca
     2641 gcgctcctac ctggagccgg gcggctacct ggacaccctg atcgccgccg gcgagatcga
     2701 tggcctgacg tccaacccct cgatcttcga gcaggcgctg ggcaaggacc gctcctacga
     2761 cgcccagctc gccgagctga aggggagcga tccccgcgag gcgctgtggg agctcatgaa
     2821 ggccgacatc acggtggcct gcgacaagtt cctgccgatg tggcgcgcct ccggcggcgc
     2881 gcacggctac gtgtcgatcg aggtggaccc gtccaaggcg ttcgacaccg ccgcgaccac
     2941 cgacgagggc tcgaagctgt tcgccgagct ggaccggccc aacctcatga tcaaggtgcc
     3001 gggcaccgag cccggcctgg aagcggtcag ccgcctgctc gccgagggcg tgaacgtgaa
     3061 cgtgacgctg ctgttctccg tggcgcgcta cgaggcggtc atcgccgcgt acctggacgg
     3121 cctgcgccgg cgggccgacc gcggcggcga cctgcgggcg gtggcgtcgg tcgcgtcgtt
     3181 cttcgtgagc cgcgtggaca gcaaggtcga cgccctgctc gggccggacc acccgcgcct
     3241 ggccacggcc gcgatcgcga acgcccgtgt ggcctacggc tcgttcgtcc gggagttctc
     3301 cgggccgggc ttcgcggacc tgcgggagct gggcgcgcgg gtgcagcgcc cgctgtgggc
     3361 gtccacgtcc acgaagaacc ccggctaccg cgacgtgctg tacgtggagg agctcgccgg
     3421 cccggacacg gtcaacacca tgccggaggt gaccctggac gccttccgcg accacggcgt
     3481 cgtcgaggat cgcatcggcg ggtcgtggga cgaggcggcg caccagctcg agcgcctggc
     3541 cgaggaaggc gtcgacctcg accagatcac caaggagctc gaggccgagg gcgtggagaa
     3601 gttcgtgcag agcttcgacg cggcggtcga cgccgtcgcc gcgcacgtct cctagcggaa
     3661 gcgcacctgc tcgctgtagt ccttgcgccc ccgtgccagc gaccccgatg gccggtcggg
     3721 cgccgggtac ccgaccgtga cgaggccgag cggcacctcc tcggggcgca ggccgagcag
     3781 ttcccgcagc ggcggggtgt cgtcgagcgc cagcaccccg gccgccaggc cctcgtccac
     3841 ggcggcgagc agcagcagca tcagcgcggc gccgccgtcc acccaccagt acggcaccgc
     3901 ccactcgtgg ggcttggtgg acggcgcctt gtccggctcc gcgtagcgct cctcgtaggc
     3961 ggcgcgcgac acgcagggca ccacatgcac gggggcggtg gacacccacg cgtcgaagcc
     4021 gcgcgcgacg tagtccgcct cgccgcacag ctcggccatg cgcgcccggg cctcctgcgc
     4081 ggtcagcacc acgaacgact ggccctgcgt gaagccggcg ctgggcgcgc gacgcgcgac
     4141 gtccagcatc cggcgcagcg cgtccggcgc caccggatcc gggcgatagg cccgcaccat
     4201 ccgccgccgc cggaccacct cggagaagtc catcgccgcg cagactaggg caatcggctc
     4261 atgcgcggga gcttcttggg gggccgaacg gcccttgccg cgcgggaccc ggaggtctcg
     4321 actcaccatc ggggtcctct gccgagcaca ggagcgcacg atgcacacct tcaccgagag
     4381 catccacatc gaacggccca ggcagcaggt ctggcgttac ctcgtggacc cggagctggc
     4441 caaggcgtgg gtcggcttcg aacagatgga cctgatcggc gaccagccgc tggccaaggg
     4501 cacgcgcatc caggaggtgt tccgcttcct cggcaagcgc atgagcatgg tcgacgaggt
     4561 cgtggagtac caggacggcg cgcacatcgc cttccgcagc gtggaggcgc ccatcgccaa
     4621 cgaggtcgac gagacgctga cggacgagga cgggggcacc cgcgtggagc tggtcatgcg
     4681 gaccgacgac acgcaagggt tcttcggcaa gctggccgag ccggtgatcg agcgcatgtt
     4741 cgcccgcgag ctgcgccagc agctgcaccg cctgaaggac ctctgcgagg cggacgcgaa
     4801 ctgacgcgcg gccgccggct cagtcgcggc cggtctccgc cacgacgggc ccggtgacgt
     4861 gcccggtgat cggcgcgagc cggcccggcc gcttcccagc gggcagctcg gcggtctcca
     4921 gcaccacggc gccgccggcg gtgccggcat gcaggtcccc gcccaccccg cagaaggcga
     4981 gccgcgccgc gcccgtctgc acgcccttgc cgccgcggcc cttggccggg tactccccca
     5041 gcgccgtgcg cttggcggcc ccgtcggcgt ccacggtgag caccggcgtg tcctcgccgc
     5101 caccgcccgg caccacggtc gccgacacga tgcgggcgcc cctgggcacg ttcatgccgg
     5161 ccacgcccgc cgccccgcgc cccatcgggc gcacctcgcc ggcggcgaag cggacgacca
     5221 gcccggccga gttcgcgagg acgatgtggt cgccgtcgtt gcacggcgcc accgcgacca
     5281 gctcgtcgtc gtcgcgcaca cccgccgcga tcatcgtgcg caggcgtccc tcgtactcgg
     5341 cgcgctcggt gcgcttcacc aggccccgcg ccgacacggt cagcacggtc tcgacgtccg
     5401 ggccgagcag cacggccccc gcgatgcgcg cgtcggggcc gtcgcccagg agctgggcga
     5461 tcgacgtgcc gcgctggcgc atggtggtca ccggcacgtc gcccacgccg atgcggtagc
     5521 cggcgccgtc cgcgtcgatc agcaggaccg tgtcctcggt ggtcgcgcgc agcacggcga
     5581 ccaccggatc gttgggatgg ctgtgcggtt tggacacccg ccgccgcggc accgccttca
     5641 ggtagccgcc ctgggtcacg tacagcgtga cgggctgcgc ctccagctcc ggcaggtcct
     5701 cgtccacgat cagctcggcg ccgcgcgtgc cggccgtccg ggcgccgggc gtcccggcgt
     5761 cgtcgtccgg cgcgtcgtcg gccgtgacga ccaggcggct gcggcgcggg ttggcgtggc
     5821 gggcgcggat ctccttgagc tcggcggcca ggagcgcgtc gagcttggcc gggtcggcca
     5881 ggatcgcctt gagctccgtg atgaccttgg tgagctcggc gtgctcggcc ttgaccttgt
     5941 cgcgctccag cttggccagg cgccgcagct gcatgtccag gatcgcggtg gcctggacct
     6001 cggtgagcga gaagcgcttc atgagctgct cgcgcgcctt gtcggcggac tccgcggcgc
     6061 ggatcagctt gatcaccgcg tccaggttgt ccagggcgac gagcaggccc tccaggacgt
     6121 gggcgcgcgc ctcggccttc tccttgcggt aggtggtgcg ccgcgtcagc acggtgcgct
     6181 ggtgggcgag gtactcggtg agtgcctcgc gcagcccgat ggtgcgcggc tggccgtcca
     6241 ccagggcgac caggttcacg ttgaagttgg tgcgcaggtc ggtgaggcgg tacagcttgc
     6301 gcaggacgcg cgccgggtcc tcgccgcgct taagctcgat caccacgcgc atgccctcgc
     6361 ggctggactc gtcgcgcagg tcgcggatct cgtcgagctt cttgtccttg accaggtcgg
     6421 ccatgcgctc cagcagcgcc gccttgttca cctggaacgg gatctcggtg accacgatgc
     6481 gcggcaggcc gccgctgcgc gtctcggtgg tcgcgaccgc ttccatggtg acggcgcccc
     6541 gaccggtgag gtaggcgtcg cggatgccgt cgccgcccac gatgcgggct cccgtgggga
     6601 agtccggcgc gggcacgtgc ttcatgagct cgtcggcggt ggcggagggc ttgcggatga
     6661 gcgccaggca ggcgtcgatc acctcggcga ggt
//
LOCUS       DATMKC010000019         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_261494, whole genome shotgun sequence.
ACCESSION   DATMKC010000019 DATMKC010000000
VERSION     DATMKC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261494"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="pyrH"
                     /locus_tag="VNU01_00635"
     CDS             <1..649
                     /gene="pyrH"
                     /locus_tag="VNU01_00635"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWH31155.1"
                     /translation="SISPDIVRSTARQLVEVSETGVQIGVVVGGGNIFRGASPQASGM
                     DRSSADHMGMLATVINALALQDACENAGLPTRVQSAVSMQELCEPYIRRRAIRHLEKG
                     RMVIFAAGLGAPYFSTDTAAAQRALEIEAEAILKGTKVDGVYDKDPAQHADAVRFDEL
                     TYLDVLSRGLKVMDATAISLCMDNRLPIIVFNILKEGNIHRVVSGEPIGTLVRVG"
     gene            670..1227
                     /gene="frr"
                     /locus_tag="VNU01_00640"
     CDS             670..1227
                     /gene="frr"
                     /locus_tag="VNU01_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWH31156.1"
                     /translation="MIEDILGEAEQKMRAAVEHAQNELSKIRTGRANPNLISDLPVEY
                     YGTPTPLQQLAGISVPEPRMLLVSPFDKTALKEIERAINGSDLGLNPSNDGNVIRVVF
                     PELTEERRRDFVKLAKERAEDGRVAIRNIRRHAKTELGKLAEAGDISEDDEHRADKRL
                     QEVTDKYVGRVDEVLSHKEKELMEV"
     gene            1321..2091
                     /locus_tag="VNU01_00645"
     CDS             1321..2091
                     /locus_tag="VNU01_00645"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWH31157.1"
                     /translation="MALAVAFIGSLVLSPWLLLAFIGVLLLIALFELDRAFREHGLRP
                     GTPVAVGAGFVMFFGSYPDGASAQTLGLVLLLFGAMAWALLDRGRTRMAASLGATMLM
                     AVWVPFLASFIGLLLARPDGRWYVFLAVLFAVFNDIGAYAVGMRFGRHKMAPTVSPAK
                     SWEGLAGGILAATVVALVVAVPLVPDLRAVHAVVLAVGASVAGTLGDLAESMVKRDLG
                     VKDLGSVLPGHGGIMDRADAIIFALPMTYLLLDALGVG"
     gene            2132..>2965
                     /gene="rlmN"
                     /locus_tag="VNU01_00650"
     CDS             2132..>2965
                     /gene="rlmN"
                     /locus_tag="VNU01_00650"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWH31158.1"
                     /translation="MDPYDASRLSELLADQPAYRLAQVRAWLSRGVDDPAVMTDLPGA
                     LRDRLADAFDPAPQVLRHSTSDGGLTHKVLLRMSGGEAVESVLMLYPRTGERAGRATV
                     CVSTQAGCAMGCPFCATGQAGFRGQLGLGEVVRQVTVMQRMLQDGAIAVPGAPDHVTN
                     VVFMGMGEPLANLDVTVDAVRWLHDPDGFNLSARSITVSTVGLVPGIRGLSQLGLPIT
                     LAVSLHAADDALRDDLVPVNRQHPIAEVMDACREYLAVTGRRLTFEYILIDGLNSSVA
                     HA"
BASE COUNT          418 a         1095 c         1022 g          430 t
ORIGIN      
        1 cagcatctcg ccggacatcg tccgctccac cgcccgccag ctcgtcgagg tctccgagac
       61 cggcgtgcag atcggcgtcg tggtcggtgg cggcaacatc ttccgcgggg cctccccgca
      121 ggccagcggc atggaccgct ccagcgccga ccacatgggc atgctcgcca cggtcatcaa
      181 cgcgctcgcg ctgcaggacg cgtgcgagaa cgcgggcctg cccacgcgcg tgcagtccgc
      241 cgtcagcatg caggagctgt gcgagcccta catccgccgc cgcgcgatcc ggcatctgga
      301 gaagggccgc atggtgatct tcgccgccgg cctgggcgcc ccgtacttct ccacggacac
      361 cgccgcggcg cagcgggcgc tggagatcga ggcggaggcg atcctcaagg gcaccaaggt
      421 cgacggcgtc tacgacaagg accccgccca gcacgccgac gccgtgcgct tcgacgagct
      481 gacctacctg gacgtgctca gccgcggcct gaaggtgatg gacgccaccg ccatctcgct
      541 gtgcatggac aaccgcctgc cgatcatcgt cttcaacatc ctgaaggagg gcaacatcca
      601 ccgggtcgtc agtggtgagc ccatcgggac cctggtgcgg gtcggctaga gacaacacgc
      661 gaggacgcaa tgatcgaaga catcctgggc gaggcggagc agaagatgcg ggcggcggtc
      721 gagcacgccc agaacgagct gtccaagatc cgcacgggcc gcgccaaccc caacctgatc
      781 tcggacctgc cggtcgagta ctacgggacc cccacgccgc tccagcagct cgcgggcatc
      841 agcgtgccgg agccgcgcat gctgctggtc tcgccgttcg acaagaccgc gctcaaggag
      901 atcgagcgcg cgatcaacgg ttccgacctg ggcctgaacc cgtccaacga cggcaacgtg
      961 atccgcgtcg tgttcccgga gctcaccgag gagcgccgca gggacttcgt gaagctggcc
     1021 aaggagcgcg ccgaggacgg ccgcgtcgcc atccgcaaca tccggcggca cgccaagacc
     1081 gagctgggca agctcgccga ggccggcgac atcagcgagg acgacgagca ccgcgccgac
     1141 aagcgcctcc aggaggtcac cgacaagtac gtcggccggg tcgacgaggt cctgtcccac
     1201 aaggagaagg agctcatgga ggtctgacct cccgagcgcg cgttcgtgac cgtggacaac
     1261 ccccaccccg caccggccgg ccccgccggc cggaatctcc ctttggccat cgtctccggg
     1321 gtggcgctgg cggtcgcgtt catcggcagc ctggtcctga gcccctggct gctgctggcg
     1381 ttcatcggtg tgctgctgct gatcgcgctg ttcgagctcg accgcgcgtt ccgcgagcac
     1441 ggcctgcggc ccggcacgcc ggtggcggtg ggcgccggct tcgtgatgtt cttcggcagc
     1501 taccccgacg gcgcgtcggc gcagaccctg ggcctggtgc tgctgctgtt cggcgccatg
     1561 gcctgggcgc tgctggaccg cggccgcacc cgcatggcgg caagcctcgg cgccacgatg
     1621 ctcatggcgg tgtgggtgcc gttcctggcg agcttcatcg gcctgctgct cgcgcggccc
     1681 gacgggcgct ggtacgtctt cctggccgtg ctgttcgcgg tgttcaacga catcggcgcc
     1741 tacgcggtcg gcatgcgctt cggccgccac aagatggcac ccacggtcag ccccgccaag
     1801 agctgggagg gcctggccgg cgggatcctg gccgccaccg tggtggcgct ggtcgtcgcg
     1861 gtgcccctgg tcccggacct gcgcgccgtg cacgcggtgg tgctcgccgt cggcgcgtcc
     1921 gtcgccggca ccctcggcga cctggccgag tcgatggtca agcgcgacct gggcgtgaag
     1981 gacctcggca gtgtccttcc gggacacggt ggcatcatgg accgggcgga cgcgatcatc
     2041 ttcgcgctgc ccatgaccta tctcctgctg gacgcgctcg gcgtgggctg agcggcgtcc
     2101 gcgcccgcgc atcccctcgc atcgagtgca catggacccg tacgacgcct cgcggctctc
     2161 cgagctcctc gccgaccagc cggcctaccg gctggcgcag gtgcgcgcgt ggctgtcgcg
     2221 cggcgtcgac gaccccgccg tcatgaccga cctgccgggc gcgctgcgcg accgcctggc
     2281 cgacgcgttc gacccggccc cgcaggtgct gcgccactcc acctccgacg gcggcctcac
     2341 gcacaaggtc ctcctgcgca tgagcggcgg cgaggcggtc gagtccgtcc tgatgctgta
     2401 cccgcgcacc ggcgagcgcg cgggccgggc gacggtgtgc gtctccacgc aggcgggctg
     2461 cgccatgggc tgcccgttct gcgccaccgg gcaggccggc ttccgcggcc agctcggcct
     2521 cggcgaggtg gtccgccagg tcaccgtgat gcagcgcatg ctccaggacg gcgccatcgc
     2581 ggtccccggc gcacccgacc atgtcaccaa cgtggtgttc atgggcatgg gggagccgct
     2641 ggcgaacctg gacgtgaccg tggacgcggt gcgctggctg cacgacccgg acggcttcaa
     2701 cctgtcggcg cgctccatca cggtgtccac cgtggggctg gtgcccggca tccgcggcct
     2761 gtcgcagctc ggcctgccga tcacgctggc cgtgagcctg cacgccgccg acgacgcgct
     2821 gcgcgacgac ctggtgccgg tgaaccgcca gcaccccatc gccgaggtca tggacgcctg
     2881 ccgcgagtac ctggccgtca ccggccggcg cctcacgttc gagtacatcc tcatcgacgg
     2941 gctcaacagc tccgtggcgc acgcc
//
LOCUS       DATMKC010000020         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_309819, whole genome shotgun sequence.
ACCESSION   DATMKC010000020 DATMKC010000000
VERSION     DATMKC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309819"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VNU01_00655"
     CDS             complement(<1..1161)
                     /locus_tag="VNU01_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWH31159.1"
                     /translation="MSWVSNLKIGRRLMLAFGALLALSVIVGGLGITKISAVDQRYGT
                     TLDLTADQLVVLGNSRADIGNEIASALRFQLNGNPKDAEAFETAAASAKKNFVALLGL
                     ATSAEGRRRIEELTGTSDRLTALFEEATALRRQGRSAEARALIAEKVAPVRVQLFEQL
                     AGYTDYRVARTEAESAALSATTRSTVWLMVGVLAVALLIGAVLAFVITRSVVRPLRRL
                     EDAVGTAAKGDLTVSVDATARDEVGQVSRSFDAMVSAVRGLVAQVADQARALSRTASD
                     MAQGSTQAGSAVSEIASTVDGVARGSGDQAKSTQEVTATVAEMGSGVQMVAEGGQRAA
                     AASEEADAAARDGVATVRHATDAMARIEHSVAGVAEVVTGLGAKSQEIGQIVG"
     gene            complement(1544..>2975)
                     /locus_tag="VNU01_00660"
     CDS             complement(1544..>2975)
                     /locus_tag="VNU01_00660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWH31160.1"
                     /translation="LPVAALLAGGRRSVRLDGDDLPRHGSAGVAVAAGFTSLAIGALR
                     MGGVLWGTVGVAGRQRLADDVPARLEEFAQLATTTIAHAEARARLAAQATTDYLTGLL
                     NHRSFHERLHAEWERARRHDRPLALVLFDIDRFKAVNDTAGHHAGDRVLEGVAERLRT
                     LARPGDVLGRIGGDEIAWLLPETDGLGAFAAAERARAHVAAAPIAHRVVTLSAGACDR
                     VDADDAEALLRLADGALYWAKAHGRDAAYLYDPEVVRELSADERAQRLAREQALVGLR
                     ALARAVDAKDPSTQRHAERVAATAVALATVLGWPEERRGALHEAGLLHDVGKIGVPDA
                     VLFKPGRLTPEEYAQVKQHAELGAEIANEVLAPEQVAWIRQHHERWDGGGYPAGLAQE
                     SISEGGRILALADAWDVMTGVRPYQVGRSVDDALRECRACGGTQFDPRLVEVLIDCVQ
                     RGTLPIAPPAGDDDRTGVVAASTRPM"
BASE COUNT          361 a         1121 c         1095 g          398 t
ORIGIN      
        1 ccccacgatc tggccgatct cctgggactt ggcgcccagg cccgtgacca cctcggccac
       61 gccggcgacg ctgtgctcga tgcgcgccat cgcgtcggtg gcgtggcgga ccgtggcgac
      121 gccgtcgcgc gcggccgcgt ccgcctcctc cgaggccgcg gcggcgcgct ggccgccctc
      181 ggccaccatc tgcacgcccg atcccatctc ggcgacggtg gcggtgacct cctgggtgga
      241 cttggcctgg tcgccggagc cgcgcgcgac gccgtcgacg gtggaggcga tctcggagac
      301 cgccgagccc gcctgggtgg acccctgtgc catgtcggac gcggtccggg acagggcgcg
      361 ggcctggtcg gcgacctgcg cgacgagacc gcgcacggcg ctcaccatcg cgtcgaagga
      421 gcgcgagacc tgcccgacct cgtcgcgcgc ggtcgcgtcc acgctgacgg tcaggtcacc
      481 cttcgcggcc gtgcccacgg cgtcctcgag ccggcgcagg ggccgcacga ccgagcgcgt
      541 gatgacgaac gcgagcaccg ccccgatgag gagggccacc gccagcacac ccaccatcag
      601 ccacacggtg ctgcgggtgg tggcgctgag ggcggcggac tccgcctcgg tgcgggcgac
      661 ccggtagtcg gtgtagccgg cgagctgctc gaacagctgc acccgcacgg gcgcgacctt
      721 ctcggcgatc agcgcgcggg cctcggcgct gcggccctgc cggcgcagcg cggtcgcctc
      781 ctcgaagagc gcggtgagcc ggtcgctggt cccggtcagc tcctcgatgc gccggcgccc
      841 ctcggcgctc gtggcgagac ccaggagcgc gacgaagttc ttcttggcgc tggccgcggc
      901 ggtctcgaat gcctcggcgt ccttggggtt cccgttgagc tggaagcgca gggcgctggc
      961 gatctcgttg ccgatgtccg cacggctgtt tccgagcacc accagctggt cggcggtgag
     1021 gtcgagcgtc gtgccgtacc gctggtcgac ggcgctgatc ttggtgatgc cgaggccgcc
     1081 gacgatgacc gacagcgcca gcagcgcgcc gaaggccagc atcagccgcc gcccgatctt
     1141 gaggttgctg acccaggaca tgggtgcgaa tcctttcgcg agttgtcggg ggacgggcgt
     1201 cccgccgccg tcgtcgtcgc ccgcggcgcc acgccgtccg cgacgggacg gggtggcggc
     1261 cctcgggccc atcggcgcgg gtcgtatgcg ggacgccacc gaatgcttcg gacggctccg
     1321 gtcgatacat aacaatcgcg ccacatggca ctgatcttgc gtcggcaatt cacgtcatcg
     1381 atgtcgtcat cgtcgccgac gccaccggca gtgggcggcg gatgatccgc cgaagctcct
     1441 gcaaaagacg gcgtcggcgt tccggcgtgc gcaccccgcg cggcgccagc ttcggcgcat
     1501 gtcatgcgct gacggcggac gtataggtcc gctccgccga acgtcacata ggacgtgtac
     1561 tggccgccac caccccggtg cggtcgtcat cgcccgccgg gggtgcgatg ggcagcgtcc
     1621 cacgctgcac gcagtcgatc agcacctcga cgagacgggg atcgaactgg gtccctccgc
     1681 acgcgcggca ctcccgcagc gcgtcgtcca ccgaccggcc cacctggtag gggcgaacgc
     1741 cggtcatcac gtcccaggcg tcggcgagcg ccaggatgcg cccgccctcg gagatggact
     1801 cctgggcgag gccggcgggg tagccgccgc cgtcccacct ctcgtggtgc tggcggatcc
     1861 acgccacctg ctccggcgcc agcacctcgt tggcgatctc ggcgccgagc tcggcgtgct
     1921 gcttcacctg cgcgtactcc tcgggggtga ggcgacccgg cttgaacagc accgcgtcgg
     1981 gcacgccgat cttgcccacg tcgtggagca gccccgcctc gtggagcgcc ccgcgccgct
     2041 cctccggcca gccgaggacg gtcgcgagcg cgacggcggt ggcggcgacg cgctcggcgt
     2101 ggcgttgggt ggaggggtcc ttggcgtcca cggcgcgcgc gagcgcgcgc aggcccacga
     2161 gcgcctgctc gcgcgccagc cgctgcgccc gctcgtcggc cgacagctcc cgcaccacct
     2221 cggggtcgta gaggtaggcg gcgtcgcgcc cgtgcgcctt cgcccagtag agcgcgccgt
     2281 cggccagccg gaggagcgcc tcggcgtcgt cggcgtcgac gcggtcgcag gcccccgccg
     2341 acagcgtcac cacgcggtgg gcgatgggcg cggcggcgac gtgggcccgg gcccgttcgg
     2401 ccgccgcgaa tgcgccgagc ccgtcggtct cgggcagcag ccaggcgatc tcgtcgccgc
     2461 cgatccggcc cagcacgtcc ccggggcgcg ccagcgtgcg cagccgctcg gcgacgccct
     2521 ccagcacgcg gtcgcccgcg tggtgcccgg cggtgtcgtt gacggccttg aagcgatcga
     2581 tgtcgaacag caccagcgcg agcggccggt cgtggcgccg tgcccgctcc cactcggcat
     2641 gcaggcgttc gtggaacgac cggtggttga ggaggccggt gaggtagtcg gtcgtcgcct
     2701 gcgccgccag ccgcgcccgg gcctccgcgt gggcgatcgt cgtcgtcgcg agctgggcga
     2761 actcctcgag ccgcgccggc acgtcgtcgg ccaggcgctg ccgtccggcg acgccgacgg
     2821 tcccccagag cacccctccc atgcgcagtg ccccgatcgc aaggctcgtg aagccggccg
     2881 ccaccgccac cccggccgac ccgtggcggg gcaggtcgtc gccgtccaga cggacggacc
     2941 ggcggccgcc ggccaggagg gccgcgaccg gcagg
//
LOCUS       DATMKC010000021         3424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_18858, whole genome shotgun sequence.
ACCESSION   DATMKC010000021 DATMKC010000000
VERSION     DATMKC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18858"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VNU01_00665"
     CDS             <1..695
                     /locus_tag="VNU01_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWH31161.1"
                     /translation="ALASGAIALFLPAHNEEAAVADVVRRAPAQVCGRAVRVLVVDDG
                     SADRTAERAELAGARLVRHGVNRGLGAAVRTGLAESVAMGAAAVAFCDADGEYAPEEL
                     ERLVAPILAGEADYVAGSRFAGEIRHMRPHRRLGNVVLTGLLRWVARAPITDGQTGYR
                     AFSREAAARAEVVHDYNYAQVLTLDLLAKGFRYREVPIGYAFRSTGASFVKLGRYLRA
                     VVPAVHRELNTA"
     gene            complement(721..2094)
                     /locus_tag="VNU01_00670"
     CDS             complement(721..2094)
                     /locus_tag="VNU01_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH31162.1"
                     /translation="MSASRDLVVLGAGPAGLAAAWWAARRGLSVTVLEQAPFVGGAAA
                     SHDIGGMRVDTGSHRLHPSTDPAILGDLRALLGEDLQRRTRNGRIRLAGRWLAFPLRA
                     ADLVRSLPPGFAAGAAWDAATGWARHPRRDDFAEVVRAGLGPTMLRRFYGPYARKIWG
                     LPPEELSGEQARRRIGANSAGGVLKRVLAAGRGEERPWFFYPRRGFGQIAEALADAAA
                     TAGAELRLSSPVTGLTLGDDDVVVGTDGGELRAGRVWSTVPLPLLARLADAPAEVQAA
                     GSRLRFRSMVLVYLVLDSDRYTAFDAHYLPGPETPVTRLSEPRNYRDSADDPPGRTVL
                     CAELPCQHGDEHWGATDEALGELVRDGLEAVGLPRPEPVEVRVRRLSHAYPIYDHASA
                     EAFATLDAWATSLQPRVLTFGRQGLFAHDNTHHALAMAKAAVQALNAPYSWTSAREAF
                     ARHVVED"
     gene            complement(2120..3214)
                     /locus_tag="VNU01_00675"
     CDS             complement(2120..3214)
                     /locus_tag="VNU01_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH31163.1"
                     /translation="MPNLSSVAERHAPDAGAAPRAEPVLVRGLHKRFGATPVLTGVDL
                     DVPAGGVVALLGPSGCGKTTLLRSIAGLERPDSGEIHLGERLLSGDGTFVPAERRRIG
                     MVFQDWALFPHLSVGRNVGFGLSREERRGGRVEEALELVGLAGLRDRAPATLSGGQQQ
                     RVALARAIANRPSVILLDEPFSNLDASLRVQVRAEVVRLLRALELTALFVTHDQEEAF
                     LLGDQVAVMLAGVIEQQAEPAELYAHPASREVATFVGDANFLPGRADGRTAHTCVGPV
                     ALRDPADGDVDVLARPEQLVVAGDHEGAPGVPAVIEAVEYYGHDAVYVVRAQDGPSFR
                     TRVIATPRFRGGDRVRVGYAGEPTIAYPAG"
BASE COUNT          434 a         1296 c         1299 g          395 t
ORIGIN      
        1 acgcgctggc cagcggcgcg atcgcgctgt tcctgcccgc gcacaacgag gaggcggcgg
       61 tcgccgacgt ggtccgccgg gcgcccgcgc aggtctgcgg gcgagcggtg cgggtgctgg
      121 tcgtggacga cggctcggcc gaccgcacgg cggagcgggc ggaactggcc ggcgcgcgcc
      181 tggtccgcca tggcgtcaac cgcggcttgg gcgccgcggt gcgcaccggc ctggcggagt
      241 cggtggccat gggcgccgcc gcggtggcct tctgcgacgc cgacggcgag tacgccccgg
      301 aggagctgga gcggctggtg gcgcccatcc tggcgggcga ggccgactac gtcgccggct
      361 cgcgcttcgc cggcgagatc cggcacatgc gcccgcatcg ccgcctgggc aacgtggtcc
      421 tgaccgggct gctgcgctgg gtggcccgcg cgccgatcac ggacggccag accggctacc
      481 gggcgttctc ccgcgaggcg gcggcgcggg ccgaggtggt gcacgactac aactacgcgc
      541 aggtcctcac gctggacctg ctggccaagg gcttccgcta ccgcgaggtg cccatcggct
      601 acgccttccg ctccaccggc gccagcttcg tgaagctggg tcgctacctg cgcgccgtgg
      661 tgcccgccgt ccaccgcgag ctcaacaccg cctgagccgc tcccgggggc gccggccgcg
      721 tcagtcctcg acgacgtggc gggcgaacgc ctcgcgggcc gacgtccacg agtacggggc
      781 gttgagcgcc tgcaccgccg cctttgccat cgccagggcg tgatgggtgt tgtcgtgggc
      841 gaacaggccc tggcggccga aggtcagcac tcgcggctgc agcgacgtgg cccacgcgtc
      901 cagggtcgcg aacgcctccg cgctggcgtg gtcgtagatc gggtaggcgt gcgacaggcg
      961 ccgcacccgc acctcgaccg gctcggggcg gggcaggccg accgcctcga ggccgtcgcg
     1021 gaccagttcg cccagggcct cgtcggtggc gccccagtgc tcgtcgccgt gctgacacgg
     1081 cagctccgcg cacagcaccg tgcgccccgg cgggtcgtcg gcgctgtcgc ggtagttgcg
     1141 cggctccgac aggcgggtca ccggcgtctc ggggccgggc aggtagtgcg cgtcgaaggc
     1201 ggtgtaccgg tcgctgtcca gcaccaggta gaccagcacc atcgagcgga agcgcagtcg
     1261 cgacccggcc gcctggacct ccgccggcgc gtccgccagg cgcgcgagga gcggcagcgg
     1321 cacggtcgac cagacacgcc cggcgcgcaa ctcgccgccg tccgtgccga ccaccacgtc
     1381 gtcgtcgccc agggtgaggc cggtgacggg actggacagg cgcagctcgg cgccggccgt
     1441 cgcggccgcg tcggcaagcg cctcggcgat ctggccgaag ccgcgccggg gatagaagaa
     1501 ccacggcctt tcctcgccgc gcccggcggc gagcacccgc ttcagcacgc cgcccgcgga
     1561 gttggcgccg atgcgccggc gcgcctgctc gccggacagc tcctcgggcg gcagtcccca
     1621 gatcttgcgg gcgtaggggc cgtagaagcg gcgcagcatc gtcggcccca ggccggcccg
     1681 caccacctcg gcgaagtcgt cgcggcgcgg atggcgcgcc cagccggtgg cggcgtccca
     1741 tgcggccccg gcggcgaagc cggggggcag gctgcgcacc aggtcggcgg cgcgcagcgg
     1801 gaacgcgagc cagcggccgg ccaggcggat gcgcccgttg cgcgtgcggc gctgcaggtc
     1861 ctcgccgagc agcgcgcgga ggtcgcccag gatcgcgggg tcggtggacg gatgcaggcg
     1921 gtggcttccg gtgtcgacgc gcatgccgcc gatgtcgtgg ctggcggcgg cgccgccgac
     1981 gaagggcgcc tgctccagca ccgtgacgga cagccctcgc cgcgcggccc accacgccgc
     2041 ggccaggccg gcgggcccgg cgccgagcac gacgaggtca cgcgaggcgc tcacggtgtg
     2101 cgcatgccgg gaagaggcct cagccggccg ggtaggcgat ggtgggctcg cccgcgtagc
     2161 ccacgcgcac gcgatcgccg ccccggaagc gcggcgtggc gatgacgcgg gtgcggaagg
     2221 acggaccgtc ctgcgcgcgg accacgtaca ccgcgtcgtg cccgtagtac tcgacggcct
     2281 cgatcacggc cggcacgccg ggcgcgccct cgtggtcgcc ggcgaccacg agctgctccg
     2341 gcctggcgag cacgtcgacg tcgccgtccg ccgggtcgcg cagggccacg ggccccacgc
     2401 aggtgtgcgc ggtgcgcccg tcggcgcggc ccggcaggaa gttcgcgtcg ccgacgaagg
     2461 tcgccacctc ccgcgacgcc gggtgggcgt acagctcggc gggctcggcc tgctgctcga
     2521 tgacgccggc cagcatgacc gccacctggt cgcccagcag gaacgcctcc tcctggtcgt
     2581 gggtgacgaa gagcgccgtc agctccagcg cgcgcagcag gcgcacgacc tcggcgcgca
     2641 cctgcacgcg cagggaggcg tcgaggttgc tgaacggctc gtccagcagg atcaccgacg
     2701 gtcggttggc gatggcgcgc gccagcgcga cgcgctgctg ctggccgccg gacagcgtgg
     2761 cgggcgcgcg gtcgcgcagg ccggccaggc ccaccagctc cagcgcctcc tccacccggc
     2821 cgccgcggcg ctcctcgcgc gacaggccga agccgacgtt gcggccgacg gacaggtggg
     2881 ggaacagggc ccagtcctgg aacaccatgc cgatgcggcg ccgctccgcc ggcacgaacg
     2941 tgccgtctcc ggagagcagc cgctcaccca gatggatctc gccggagtcc gggcgctcca
     3001 gccccgcgat cgagcgcagc agcgtggtct tgccgcagcc cgacgggccc agcagcgcga
     3061 cgacaccgcc ggcgggcacg tccaggtcca cgcccgtgag caccggggtg gcgccgaacc
     3121 gcttgtgcag gccgcgcacc aggacgggct ccgcgcgcgg cgccgcgccc gcatccgggg
     3181 cgtgacgctc ggcgacgcta ctcaggttag gcatccctaa gaaggtagcc ccgctccaag
     3241 tgcgcggcaa attcgcaaca aggaggttgt gatttcccgc gagccggccc caggccaatc
     3301 cggcatgccc gccgctccga acgcggggag acgtcccact cactcaagga gcccctccat
     3361 gcgtctcacg cccgccttcc gcagcctggt cctcggcctg gccgccgcac tgaccctgac
     3421 cgcc
//
LOCUS       DATMKC010000022         3334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_309503, whole genome shotgun sequence.
ACCESSION   DATMKC010000022 DATMKC010000000
VERSION     DATMKC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309503"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VNU01_00680"
     CDS             <1..587
                     /locus_tag="VNU01_00680"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HWH31164.1"
                     /translation="GALYVWAFERQAGRYGIGLSDRLLRFVGGSSVFLAFSAVPLYAL
                     WATYWDFLSRGEPVPLWLGLVPPTYVLLPVLAGTIVGLGLRHGWKWSDVLTGPDPAPR
                     AWDYLFQYQIDGWVRCRMKSGTWLAGAYADANGRRSYAAGYPEPQDIYLAASVAVDSD
                     TGEFLLDETGRPQRGTGGLLLRWEEVEYLEFIDS"
     gene            complement(786..858)
                     /locus_tag="VNU01_00685"
     tRNA            complement(786..858)
                     /locus_tag="VNU01_00685"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Ile,seq:cat)
     gene            complement(905..977)
                     /locus_tag="VNU01_00690"
     tRNA            complement(905..977)
                     /locus_tag="VNU01_00690"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(942..944),aa:Asn,seq:gtt)
     gene            1111..1389
                     /locus_tag="VNU01_00695"
     CDS             1111..1389
                     /locus_tag="VNU01_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31165.1"
                     /translation="MMRLIRPGMMLGFAAGYVLGAKAGRERYYQIEAMWRKAMKSPQA
                     QQLTSEMRQTAHTVGDVVEQKASEGVSQVASTLNQARDKVEQTIDRSS"
     gene            complement(1452..2423)
                     /locus_tag="VNU01_00700"
     CDS             complement(1452..2423)
                     /locus_tag="VNU01_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWH31166.1"
                     /translation="MLALTAAACGDRAEPVAVTPTTERPATAAPAPDGWFAVAPALLD
                     VTEVAAAAFAGAVWVAGGLDTRGQAARDVQVYDPAFDTWSPGPELLEPLHHTALVSTG
                     DRLYLIGGYRGPGYDAPTASVWVLDSETGDWTEGPALPEPRAAGAAAWDGERLVYGGG
                     VGADRSPTADVFALEGEQWRRIGELSSPREHLAAASDGEGTVWFMGGRRGGDLSTNVA
                     TVELVVGDRIAAGPPVPTPRGGVAAFWAPETGACLLGGEASSGTYARVECVREDGTVH
                     TLTRMAQPRHGLGVAVLDGTVYAALGGPTPGLSATAIVEALHLGSLD"
     gene            complement(2449..>3334)
                     /gene="rpoD"
                     /locus_tag="VNU01_00705"
     CDS             complement(2449..>3334)
                     /gene="rpoD"
                     /locus_tag="VNU01_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWH31167.1"
                     /translation="KEIGRVPLLTAEEEVDLAKRVEAGIVATEIVTEHEEHLPADWVG
                     ELHRIERGGQMAKRKLVESNLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDY
                     TKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRTQRQMLQDTGREPT
                     DEELGRELEMSPERVREILKISQEPVSLETPVGEEDDSNLGDFIEDHDAVVPVDAASF
                     VLLQEQIEGILHTLTEREKKVIQLRFGMLDGQPRTLEEVGIEFGVTRERIRQIESKTL
                     SKLRHPSRSQKLRDYLDD"
BASE COUNT          482 a         1200 c         1140 g          512 t
ORIGIN      
        1 ctggggcgct ttacgtctgg gctttcgagc gccaggccgg acgttatggc attggcctca
       61 gcgaccggct gttgcgcttc gtcggcggtt cctccgtgtt cttggcgttc agcgccgtcc
      121 cgctgtacgc gctctgggcc acgtactggg actttctgtc gcggggcgaa cccgtgccgc
      181 tgtggctggg actagtgccg cccacatacg tcctcctgcc agtgctggcg gggacgatcg
      241 tggggcttgg cctgcggcac ggttggaagt ggtccgacgt cctgaccgga ccagatcctg
      301 ctccacgcgc ctgggattac ctgtttcagt accagatcga cggctgggtg cggtgccgca
      361 tgaaatcagg tacatggctt gcgggcgcat acgccgacgc caacgggcgc aggtcgtacg
      421 cggctggcta tcccgagcca caagacatct acctcgccgc ctcggtggcg gtcgacagcg
      481 acacgggaga gttcctgctc gatgagaccg gccggcccca acgcggcact gggggcctgc
      541 tgttacggtg ggaggaggtg gaatatcttg agttcatcga ctcgtgaccc aggaggcggc
      601 atggccggga agcctgacgg gggcaggaac atcggctccg gtgagaagcg cggaggctat
      661 gtctcctcct cgaagcccgc gtcggcgttg aagccgccgc ctgccgggcc ggcgccgggc
      721 gcctccaagc cgagcggtac tgcgcagaag ggctgacgcg gaaagttcgc accctggcaa
      781 gttcgtgggc tcgggaggga tcgaacctcc gtctctcgga ttatgagtcc ggtgctctga
      841 ccgctgagct acgagccctg gaaacacgac ggccccgtcg ctcgaggaga acacggggcc
      901 agggctctgg gggtgggact cgaacccaca acctaccggt taacagccgg ttgctctgcc
      961 ggttgagcta ccccagatcg gcacaacggg cgcgagtata gccgctccct gaccgctcgg
     1021 ccgggcgcgg cgcggtcatc ccgcggttcc cggagcgcaa ggaagggaag gcaccaggca
     1081 cgtcctcgca acgttgacaa ggagtgtcga gtgatgcgcc tgatccgacc cggcatgatg
     1141 ctcggcttcg ccgcgggcta cgtcctcggc gccaaggccg gccgagagcg ctactaccag
     1201 atcgaggcca tgtggcggaa ggcgatgaag tcgccgcagg cccagcagct gaccagcgag
     1261 atgcgccaga ccgcgcacac cgtgggcgac gtggtcgagc agaaggcttc ggagggcgtc
     1321 tcgcaggtcg cctccacgct gaaccaggcg cgcgacaagg tcgagcagac gatcgaccgc
     1381 tcctcctgac cggagcgttg aaccgttcgt ccgagatccc ggacgaacgt tccaggttgg
     1441 gagagcgggg gtcagtccag gctgccgagg tgcagcgcct cgacaatggc ggtggccgac
     1501 agccccggcg tcggcccgcc gagcgccgcg tagaccgtgc cgtcgagcac cgccacgccc
     1561 agcccgtgcc gcggctgcgc catgcgcgtc agcgtgtgga cggtgccgtc ctcgcgcacg
     1621 cactccacgc gggcataggt cccggaggac gcctcgccgc ccagcaggca ggcgcccgtc
     1681 tccggcgccc agaacgccgc cacgccgccg cgcggcgtgg gcaccggcgg gccggccgcg
     1741 atgcggtccc ccaccaccag ctccacggtg gccacgttgg tcgacaggtc gccgccgcgc
     1801 cgcccgccca tgaaccacac cgtgccctcg ccgtcgctgg cggcggccag gtgctcgcgc
     1861 ggcgacgaca gctcgccgat gcgccgccac tgctcgccct ccagcgcgaa gacgtccgcc
     1921 gtcggtgagc ggtcggcgcc gacaccgccg ccgtagacca gccgctcgcc gtcccacgcc
     1981 gcggcccccg cggcgcgcgg ctccggcagc gccggcccct ccgtccagtc gccggtctcc
     2041 gagtccagca cccacacgct ggccgtcggg gcgtcgtaac cggggccgcg gtagccgccg
     2101 atcaggtaca gccggtcgcc ggtgctcacc agcgccgtgt gatgcaacgg ctcgagcagc
     2161 tccggaccgg gcgaccaggt gtcgaacgcg gggtcgtaga cctgcacgtc gcgggccgcc
     2221 tgtccccggg tgtccagccc gccggccacc cacacggccc cggcgaacgc cgcggccgcg
     2281 acctcggtga cgtccagcag cgccggcgcc accgcgaacc agccgtccgg cgcgggcgcc
     2341 gcggtcgcgg gccgctcagt ggtgggcgtg accgcgaccg gttccgcccg gtccccgcag
     2401 gcggccgccg tgagcgccag caccagcagg acggccaggc gcgccggcct agtcgtccag
     2461 atagtcgcgc agcttctgac tccggctggg gtgccggagc ttggacagcg tcttgctctc
     2521 gatctgacgg atgcgctcac gggtgacgcc aaactcgatc ccgacttcct ccaaggtgcg
     2581 cggctggccg tcgagcatgc cgaagcgcag ctggatgacc ttcttctcac gctcggtgag
     2641 cgtgtgcagg atcccctcga tctgctcctg aagcaaaacg aacgacgccg cgtcgaccgg
     2701 caccacggcg tcgtggtcct cgatgaagtc gcccaggttg gagtcgtcct cctcgcccac
     2761 cggcgtctcc aggctcaccg gctcctggct gatcttgagg atctcgcgca cgcgctccgg
     2821 cgacatctcc agttctcggc ccagctcctc gtcggtcggc tcccgccccg tgtcctgcag
     2881 catctgccgc tgcgtgcgga tcagcttgtt gatcgtctcc accatgtgca ccgggatgcg
     2941 gatggtgcgg gcctggtcgg cgatcgcgcg cgtgatcgcc tggcggatcc accaggtggc
     3001 gtacgtcgag aacttgtagc ccttggtgta gtcgaacttc tcgaccgccc ggatcagccc
     3061 caggttgccc tcctggatga ggtccaggaa cagcatcccc cgccccacgt agcgcttggc
     3121 gatggacacc accaggcgca ggttggactc caccagcttg cgcttggcca tctgcccgcc
     3181 gcgctcgatg cggtgcaatt ccccgaccca gtcggccggc aggtgctcct cgtgctccgt
     3241 gacgatctcc gtggcgacga tcccggcctc gacgcgcttg gcgaggtcga cctcctcctc
     3301 ggcggtcagg agcggcacgc ggccgatctc cttg
//
LOCUS       DATMKC010000023         9340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_286295, whole genome shotgun sequence.
ACCESSION   DATMKC010000023 DATMKC010000000
VERSION     DATMKC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286295"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1271)
                     /locus_tag="VNU01_00710"
     CDS             complement(60..1271)
                     /locus_tag="VNU01_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWH31168.1"
                     /translation="MDVFVARQPILDAEQRVYGYELLFRSGLENAFPGTDPTLASTKV
                     LTSSVLSIGLDLLTRGKPAFVNFTRDGLLADDAGFLPSASAVVEIVEDIEPDADVVAA
                     CRRLRELGYRLALDDFVYRPELEPLLELASFVKVDFERTTAGERLALVERLRDRPVSL
                     VAEKVETVADYREGLSLGCSHFQGYFFSRPVVYRGQDLRGHRGSQMQLMHEAGRDELD
                     FARVERIVKTDIALSYKLLRYINAVSFGWKQRITSVRHALSLLGQEQVRRWALLVALS
                     SMAERRPPELSVMSAVRGRLCEGLLTLGSAGGDRALDGFMLGMFSLIDALLEQPMAQA
                     LEGVPLAEDVHAALLGEPNRARQVLDTVVAYERGDWSALDERAARLGLPEERLPRLYA
                     EAVEWAGAVFD"
     gene            1463..1993
                     /locus_tag="VNU01_00715"
     CDS             1463..1993
                     /locus_tag="VNU01_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31169.1"
                     /translation="MAGAAASRRQWTSCVAAVVVLIALLTLVFPVVRALAGPQPAAPA
                     SSPVLVRVTPATGALGGGRLEPGVADYRPVEVHNDGTVAFRYDLTVSARGPLVGIVLV
                     EFRGVARHCDAATFAAGERVAGPAPMGLLVREGGRTLAPGASELLCARVELPDGARTP
                     SAEPTDAVLRVSAVQR"
     gene            complement(2015..4354)
                     /locus_tag="VNU01_00720"
     CDS             complement(2015..4354)
                     /locus_tag="VNU01_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH31170.1"
                     /translation="MRGAALLVAVTLCAALGSPAPPARAVARAPAADPVPGGLLVTTE
                     TAEGADALLAALASPLPPLPGAAARRLTRRVLGVRVAAGGEQAAAEALRALPGVDAVE
                     REGWMRLQRVPDDSRYADQWAHQRTGAEAAWDDSTGDARVRVAVIDSGILGSHPDLAP
                     NVTEDQVSTAGGTATVTGLRVDNDPCNAGHGTFVAGVLGATGDDGLGIAGVAWDVSIV
                     DVSALSAENCDGLADSAILRGLDYALRNPAGPVDVVNMSFGRPADACPVSYHTAIEDL
                     RAAGVVVVAAAGNGEANPDTAGTAMIPASCDGAISVAASRRDDGTASYSSRNPFVDLT
                     APGGEDTGDGDGLILSTAMDQQGRAGYSRQAGTSHAAPYVSGAAALLRAVEPELTPDQ
                     VEWALEQGAKDLARPGRDNPTGWGLVRLDASLALAGGRAGTPEPDPSFPVDETSRPWS
                     PPRVARVAAQGATTAPVSQAVAVSRTLFGAGEAMHAVLAREDVFADALAGSALGNGLG
                     PLLFTGRTGSLAGATRAELLRVLPAGATVYLLGGSAALPVSLEQELADDGLRPVRLSG
                     PSREQTAVAVAQEVDRLRLRAQRAILLARSDQWPDAVAAGSLAAWFGMPVLLTAPTAL
                     HPATAAALHALGPDRLYALGGSAAIAPEVLDAAGEAAGVPASERRRLAGPGRAETAVA
                     IASEMEALLREHGDGPPGGVLAVNLRRADGFAHALSASALAGAIAGVMVGVEGTNGQT
                     LADAVRGYLEGFGVDGYVMGGADVVAEPVRQELEALLRG"
     gene            4603..7200
                     /locus_tag="VNU01_00725"
     CDS             4603..7200
                     /locus_tag="VNU01_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWH31171.1"
                     /translation="MGPDPLRALLATAPFGIALVDRDLRYLEVNDYLARLNGLPADAH
                     VGRTLREVAPRVADELERHGRAVLDTGAAVEDVRLHDRQGEDDAREWLLSLHPVHGPA
                     GEVTAVGAVVVDVTDAARTARQLDRRTTQQAVVARFGQRALAGADVDDLRDEAASLAA
                     ATLGVEFGEVLELVPGDTALVVRAGVGWRDGVVGTRIQAGPQTQAGTVVDTRAAVVVE
                     NLAVERRFASVGELREHGVVSGASVPIQSQGTLHGVLAAHSTKPRRFSTDDVAFLQAL
                     ANVLGAAIDRAQAEDRLRESAQRLHLALQAGRLGTWEWDVESGHVRWSETLEAVYGLP
                     AGAFAGTYEDYLARLHPEDRDRVAGLIRDALADGASLPEFDHRIVRPDGSVRWLRCSG
                     GPVRRPDGRIGGMLGLAMDVTEHKLAEQERLRLLEAEQSARAVAEAAHERLAFLARVT
                     AALNTTLDVEELLGKVTSLALGWFADGCAVDLLEDGALRRAVLSHRDPVRAEAYRELR
                     RLYPPRPGEGNALLRVVETRKPLLIPLVSEKFQRAYALDERHLGLMRVVAARSAMCVP
                     LVARDRVLGLLNFVRDEERPFDQDALELAEDVARRLALAIDNAMLFQERAEGERRFRR
                     LAETLQGSLLPPHLPDIPGMELAAAYRPAAKGLEVGGDFYDVFPLEGDHWGIVMGDVC
                     GKGPEAASLTAFARYTLRAAAVQHRQPSRAVMALNKALLRDGRHDAGRFLTVAFARLR
                     PTASGVELSLVLGGHPPPLVVRGDGLVEAAGTPGTLVGVVDEIELTDAVVTLAPGDLL
                     LCFTDGVTEARRGGELFGDGRLARLAADSAGLAPDKVLERVEQALDEFSCGMSRDDTA
                     LLALRVSSH"
     gene            complement(7259..7948)
                     /locus_tag="VNU01_00730"
     CDS             complement(7259..7948)
                     /locus_tag="VNU01_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH31172.1"
                     /translation="MTNIVLIESSPNVRQEVTAALNGDAGDLDLCEVQPDAEALSSVS
                     EQRPDLVLVDRRIGAAQVPQLLGRLRAITDSPLIVINVDNRKAEKLSLLEAGADDYVS
                     RPLDRDALRRRIRAALGYRDRTPERQLKIDGLEIDFDQQRVVVQGAEVHLTPTEFSLL
                     RELASRPGGLLTHTQLLRRVWGPAYGTESHYLRVYVAQLRRKLGDNAAQPRLIVTEPG
                     IGYRWIGGETQ"
     gene            8233..8445
                     /locus_tag="VNU01_00735"
     CDS             8233..8445
                     /locus_tag="VNU01_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31173.1"
                     /translation="MPESASTPRRRVERAMSLSSGKPMTQITSARACAYPGCETKLSR
                     YNPNPCCAVHGGWADEEGGRRSRNSV"
     gene            8499..8762
                     /locus_tag="VNU01_00740"
     CDS             8499..8762
                     /locus_tag="VNU01_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31174.1"
                     /translation="MDLGGAMFGGLAVLVVLVLAAVYVGARLHGGKAPSHARDNEHAG
                     RSPAGDEDPVPRDSDGMPMVTPPEDPAETSVDEMQRRARRNAS"
     gene            8759..9148
                     /locus_tag="VNU01_00745"
     CDS             8759..9148
                     /locus_tag="VNU01_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWH31175.1"
                     /translation="MTDAQRFRALLEEERDRLRAIRGGADGHLSDSQEASTGELTTFD
                     QHPGDVASETLEREQDESIREHAEAQLEEVEAALRRLDDGSYGRCAVCGADIGEARLQ
                     VRPQTRYCVEHQHERERTRGAERQPRA"
BASE COUNT         1142 a         3522 c         3540 g         1136 t
ORIGIN      
        1 cctctgcctc ccgccatccg cgctgttcta ccgcctctcg ccgcccgcgc ccggcaggct
       61 cagtcgaaca ccgcgccggc ccactccacc gcctcggcgt acagccgtgg cagccgctcc
      121 tccggcaggc ccagccgcgc cgcccgctcg tccagggccg accagtcgcc gcgctcgtac
      181 gccacgacgg tgtccagcac ctggcgggcg cggttcggct cgcccagcag ggcggcatgc
      241 acgtcctcgg ccagcggcac gccctccagc gcctgcgcca tcggctgctc caggagcgcg
      301 tcgatgagcg agaacatgcc cagcatgaaa ccgtccagcg cgcggtcgcc gcccgccgag
      361 cccagcgtga gcagcccctc gcacagccgc ccgcgcacgg ccgacatgac cgacagctcc
      421 ggcgggcgcc gctcggccat gctcgacagc gccaccagca gcgcccagcg gcggacctgc
      481 tcctgcccca gcagcgacag ggcgtggcgc accgaggtga tgcgctgctt ccagccgaac
      541 gacaccgcgt tgatgtagcg cagcagcttg taggacagcg cgatgtcggt cttgacgatg
      601 cgctcgaccc gtgcgaagtc gagctcgtcc cggcccgcct cgtgcatcag ctgcatctgg
      661 ctgccacggt gcccgcgcag gtcctggccg cggtagacca ccggccggct gaagaagtag
      721 ccctggaagt gcgagcagcc cagcgacagc ccctcgcggt agtccgcgac cgtctcgacc
      781 ttctcggcga ccagggagac cggacggtcc cgcaggcgct ccaccagcgc caggcgctcg
      841 ccggccgtgg tgcgctcgaa gtcgaccttg acgaagctgg ccagctccag cagcggctcc
      901 agctccggcc ggtagacgaa gtcgtccagg gccaggcggt agcccagctc ccgcagccgg
      961 cggcaggcgg ccacgacgtc ggcgtccggc tcgatgtcct cgacgatctc gacgacggcg
     1021 ctcgcgctcg gcaggaaccc ggcgtcgtcg gccagcagcc cgtcgcgggt gaagttgacg
     1081 aaggccggct tgccgcgcgt gagcaggtcc aggccgatgg acagcacgct ggaggtgagc
     1141 accttggtgg aggccagcgt ggggtccgtg ccggggaagg cgttctccag ccccgagcgg
     1201 aacagcagct cgtagccgta gacccgctgc tcggcgtcca ggatcggctg ccgagccacg
     1261 aacacgtcca tcccgacccc ttgcccagcg gcgcttgcgc accgcgcttc accgtaacaa
     1321 agcggcgtcc ttccggtaag ccggacagaa aactcgttcc cgggcccccg cggcgtcagc
     1381 gggcgggccc gcgctccgta tcagcgcacg ggatgggagc gtgcggccgt cgaggccgcc
     1441 gcacggccag gagcttcaca tcgtggcagg cgcggcagcg agccggcggc agtggacgtc
     1501 gtgcgtggcg gctgtggtgg tgctgatcgc gctgctcacg ctggtgttcc cggtggtcag
     1561 ggcgctggcc gggccccagc ccgccgcacc ggcgagttcg ccggtgctcg tgcgcgtgac
     1621 accggcgacg ggcgcgctgg gcggcgggcg cctggagccc ggcgtggcgg actaccggcc
     1681 ggtcgaggtc cacaacgacg gcacggtggc gttccgctac gacctcacgg tgtcggcccg
     1741 gggaccgctg gtgggcatcg tgctggtgga gttccggggc gtggcccgcc actgcgacgc
     1801 ggcgacgttc gccgcggggg agcgcgtcgc gggtcccgcg ccgatgggcc tgctggtccg
     1861 cgagggcggc cgcacgctgg cgcctggcgc gtcggagctg ctgtgcgccc gcgtggagct
     1921 gccggacggc gcgcgcacgc cgtcggcgga gccgaccgac gccgtcctgc gggtctccgc
     1981 ggtccagcgc tgaggctcct ggcggcccgc cgcctcaccc gcgcagcagc gcctccaact
     2041 cctgccgcac cggctcggcg accacgtcgg ctccgcccat gacatagccg tccaccccga
     2101 agccctccag gtagccgcgc acggcgtccg ccagcgtctg cccgttggtg ccctccacgc
     2161 cgaccatcac gccggcgatc gcgccggcca gtgcggacgc cgacagcgcg tgggcgaacc
     2221 cgtcggcacg gcgcaggttc acggccagca ccccgcccgg cgggccgtcg ccgtgctcgc
     2281 gcagcagcgc ctccatctcc gaggcgatgg ccaccgcggt ctcggcccgg ccgggtccgg
     2341 ccagccgccg ccgttcgctc gcgggcacgc cggcggcctc gccggcggcg tccaggacct
     2401 ccggggcgat ggccgcgctg ccacccagcg cgtacagccg gtccgggccc agcgcgtgca
     2461 gcgcggcggc ggtggccggg tgcagcgccg tgggggcggt cagcaggacg ggcatgccga
     2521 accaggcggc cagggagccg gccgccacgg cgtcgggcca ctggtccgac cgcgcgagca
     2581 ggatcgcccg ctgcgcccgc agccgcagcc ggtccacctc ctgggccacc gcgaccgcgg
     2641 tctgctcgcg cgacgggccg gacagccgca cgggccgcag gccgtcgtcg gcgagctcct
     2701 gctccaggct cacgggcagc gcggcgctgc cgccgagcag gtacaccgtg gcgccggccg
     2761 gcagcacgcg cagcagctcg gcgcgcgtcg cgccggccag cgagccggtc cggccggtga
     2821 acagcagcgg cccgaggccg ttgccgagcg cgctgcccgc cagcgcgtcg gcgaagacgt
     2881 cctcgcgcgc cagcacggcg tgcatcgcct cgccggcgcc gaacagcgtg cgcgacaccg
     2941 ccaccgcctg gctgaccggc gcggtggtcg ccccctgcgc ggccacgcgc gcgacccgcg
     3001 gcggcgacca cggccgggac gtctcgtcca cggggaagga ggggtcgggc tccggcgtgc
     3061 ccgcgcggcc gcccgccagc gccagcgacg cgtccagtcg cacgaggccc cacccggtcg
     3121 ggttgtcgcg gcccggccgg gcaaggtcct tggcgccctg ctccagcgcc cactcgacct
     3181 ggtcgggggt gagctccggc tccaccgcgc gcagcagggc ggcggcgccg ctgacgtacg
     3241 gggcggcgtg ggacgtgccg gcctgccgcg agtagcccgc ccggccctgc tggtccatcg
     3301 ccgtggacag gatcaggccg tcgccgtcgc cggtgtcctc gccgccgggg gcggtcaggt
     3361 ccacgaacgg gttcctgctg gagtaggagg cggtgccgtc gtcgcgccgg ctcgcggcga
     3421 ccgagatcgc cccgtcgcag gacgccggga tcatcgccgt gccggcggtg tcgggattgg
     3481 cctccccgtt gccggcggcc gcgacgacca ccacccccgc cgcgcgcagg tcctcgatcg
     3541 cggtgtggta gctgacgggg cacgcgtccg ccgggcgccc gaagctcatg ttcaccacgt
     3601 ccaccggccc ggccgggttg cgcagcgcgt agtccagccc gcgcaggatc gccgagtcgg
     3661 ccagcccgtc gcagttctcg gccgacaggg cggagacgtc gacgatggac acgtcccagg
     3721 cgacgcccgc gatgccgagc ccgtcgtcgc cggtggcgcc gagcacgccc gcgacgaagg
     3781 tgccgtggcc cgcgttgcag gggtcgttgt cgacccgcag ccccgtcacg gtcgccgtgc
     3841 ccccggcggt ggacacctgg tcctcggtga cgttgggcgc caggtccggg tgcgagccca
     3901 ggatgccgct gtcgatcacc gcgacccgca cgcgcgcgtc accggtgctg tcgtcccagg
     3961 ccgcctcggc gccggtgcgc tggtgcgccc actggtcggc gtagcggctg tcgtccggca
     4021 cccgctgcag gcgcatccag ccctcgcgct ccacggcgtc cacgccgggc agcgcgcgca
     4081 gcgcctccgc ggccgcctgc tcgccgccgg cggcgacgcg caccccgagc acccggcggg
     4141 tcagccgccg cgcggcggcg cccggcagcg gtggcagcgg ggatgcgagc gcggccagga
     4201 gcgcgtccgc cccctccgcc gtctccgtcg tgaccagcag cccgccgggc acggggtccg
     4261 ccgccggcgc tcgggcgacc gcgcgggccg gcggggcggg ggagccgagc gccgcgcaca
     4321 gcgtcacggc gacgagcagc gccgcaccgc gcatcctgtc caacctccgc gtcgaccacc
     4381 gcgtcgacca ccgcaccaac gggccggctc ccgggccggc accgccgcag ggtaaccgag
     4441 ggacggcggc cgggtccccg gactgccgcc gggaatttcc gtgcgccgcc gtgcggcacc
     4501 ggcttaccat cgccgcaccc ctcccccccg gggggcggag gggctgccgg agccgtgccg
     4561 gcgtggtgcg gcgactcccg ccggcggtga ccaggaggcg aggtgggacc tgatccgctg
     4621 agagcactgc tggcgaccgc accgttcggg atcgccctgg tcgaccgcga cctgcgctac
     4681 ctggaggtca acgactacct cgcccgcctc aacggcctgc ccgcggacgc ccacgtgggc
     4741 cgcaccctgc gcgaggtggc gccgcgcgtc gccgacgagc tggagcgcca cgggcgcgcg
     4801 gtcctcgaca ccggcgcggc cgtggaggac gtgcgcctgc acgaccggca gggcgaggac
     4861 gacgcgcgcg agtggctgct gagcctgcac cccgtgcacg ggccggccgg cgaggtgacc
     4921 gccgtcggcg ccgtggtggt cgacgtcacg gacgccgcgc gcacggcgag gcagctcgac
     4981 cgcaggacca cgcagcaggc ggtggtcgcg cgcttcggcc agcgcgcgct ggccggcgcc
     5041 gacgtggacg acctgcgcga cgaggcggct tccctggccg ccgccacgct gggcgtggag
     5101 ttcggcgagg tgctggaact ggtgcccggg gacacggccc tggtcgtgcg ggcgggcgtg
     5161 ggctggcgcg acggcgtggt cggcacgcgc atccaggcgg ggccgcagac gcaggccggc
     5221 acggtcgtgg acacccgggc ggccgtggtc gtggagaacc tcgccgtgga gcggcgcttc
     5281 gcctccgtgg gggagctgcg ggagcacggc gtcgtcagcg gcgcttcggt gcccatccag
     5341 tcccagggca cgctgcacgg ggtgctggcg gcgcactcga ccaagccgcg gcgcttctcc
     5401 accgacgacg tggcgttcct ccaggcgctg gcgaacgtcc tgggcgcggc catcgaccgc
     5461 gcccaggccg aggaccgcct gcgggagagc gcgcagcgcc tgcacctggc gctgcaggcg
     5521 ggccggctgg gcacgtggga gtgggacgtc gagtccggtc acgtgcgctg gtcggagacg
     5581 ctcgaggcgg tctacgggct gcccgcgggc gcgttcgccg gcacctacga ggactatctg
     5641 gcgcgcctgc acccggagga ccgtgaccgc gtggccggcc tgatccgcga cgcgctcgcc
     5701 gacggggcct cgctgccgga gttcgaccac cgcatcgtgc gcccggacgg ttccgtgcgc
     5761 tggctgcgct gcagcggcgg cccggtgcgc cgccccgacg gccgcatcgg cgggatgctc
     5821 ggcctggcca tggacgtcac ggagcacaag ctcgcggagc aggagcggct gcggctgctg
     5881 gaggccgagc agagcgcgcg cgccgtggcc gaggcggccc acgagcgcct ggcgttcctg
     5941 gcccgcgtga ccgcggcgct gaacacgacg ctggacgtgg aggagctgct gggcaaggtc
     6001 acgtccctgg ccctgggctg gttcgccgac ggctgcgccg tcgacctgct ggaggatggc
     6061 gcgctgcggc gggcggtgct gagccacagg gacccggtga gggccgaggc gtaccgggag
     6121 ctgcggcgcc tgtacccgcc gcgccccggc gagggcaacg cgctgctgcg cgtcgtggag
     6181 acgcgcaagc cgctgctgat cccgctggtc agcgagaagt tccagcgcgc ctacgccctc
     6241 gacgagcgcc acctggggct gatgcgggtc gtggccgccc gctccgcgat gtgcgtgccg
     6301 ctggtcgcgc gcgaccgcgt gctggggctg ttgaacttcg tgcgcgacga ggagcgcccg
     6361 ttcgaccagg atgcgctgga gctggcggag gacgtcgccc ggcgcctcgc cctggccatc
     6421 gacaacgcca tgctgttcca ggagcgcgcc gagggcgagc gccgcttccg ccgcctggcg
     6481 gagacgctcc agggcagcct gctgccgccg cacctgccgg acatccccgg catggagctc
     6541 gcggcggcgt accggcccgc cgcgaagggg ctggaggtcg gcggcgactt ctacgacgtg
     6601 ttcccgttgg aaggcgacca ctggggcatc gtcatgggcg acgtctgcgg gaaggggccg
     6661 gaggcggcca gcctgaccgc gttcgcccgc tacaccctgc gcgccgccgc ggtgcagcac
     6721 cgccagccga gccgcgccgt gatggcgctc aacaaggcgc tgctgcgcga cggccgccac
     6781 gacgcgggcc gcttcctcac ggtggcgttc gcgcgcctgc ggcccaccgc ttccggtgtc
     6841 gagctctccc tggtgctggg cggccatccg ccgccgctcg tcgtgcgcgg cgacggcctg
     6901 gtggaagcgg ccggcacgcc cggcacgctg gtgggggtgg tcgacgagat cgagctcacc
     6961 gacgccgtgg tcacgctcgc gccgggtgac ctgctgctgt gcttcaccga cggcgtgacg
     7021 gaggcgcgcc gcgggggcga gctgttcggc gacggccggc tggcgcgcct ggccgcggac
     7081 agcgcggggc tggctcccga caaggtgctg gagcgcgtgg agcaggcgct ggacgagttc
     7141 agctgcggca tgtcccgtga cgacacggca ctgctcgcgc tgcgcgtcag ctcacactga
     7201 tcgcgcgcgg catggccccg gcggggcgct cagccgaact ggacgccgtg ctgctgtgtc
     7261 attgcgtctc tccgccgatc cagcggtagc cgattcccgg ctccgtcacg atgagccgcg
     7321 gctgcgcggc gttgtcgccc agcttgcgcc gcagctgcgc cacgtaaacc cgcaggtagt
     7381 gcgactcggt cccgtaggcg gggccccaca cgcgccgcag cagctgggtg tgcgtgagca
     7441 ggccgccggg acggctggcc agctcacgca gcaggctgaa ctccgtgggc gtgaggtgga
     7501 cctcggcgcc ttggaccacg acgcgctgct ggtcgaagtc gatctccagg ccgtcgatct
     7561 tcaactgccg ctccggggtg cggtcgcggt aacccagggc cgcgcggatc cgcctgcgca
     7621 gcgcgtcgcg gtccagcggc cgggagacgt agtcgtccgc ccccgcttcc agcaggctga
     7681 gtttctccgc cttgcggttg tcgacgttga tcacgatcag cggggagtcg gtgatcgcgc
     7741 gcaggcggcc cagcagctgc ggcacctggg cggcgccgat gcgccggtcc acgagcacga
     7801 ggtccgggcg ctgctcggac acgctgctca gcgcctcggc gtccggctgg acctcgcaca
     7861 ggtcgaggtc gccggcgtcg ccgttcagcg cggcggtgac ctcctgtcgc acgttcgggg
     7921 aggactcgat gagcacgatg ttcgtcacag gctccctctt ggttcgcggc gggtcgcgcg
     7981 ccacagcgcc tcggcagccg cggcacacgg cgggacggtg cccgccgctg cgcgcctcta
     8041 acccccaact accccggcga tggccggcgg gaacctgtcc gggctgtcct gacgggcctg
     8101 tcaggacgcg cgggtcgccg tttcggcgac ggtgcgctcg ggaagggaag ggacgactac
     8161 tacagcgccg ggttggcgcg tgcgcccgag cgcccgccgt ccggcgtccc ccatcgcagt
     8221 ctggaggaac gcatgcccga gagcgcgtcg accccgcgcc gccgcgtcga acgggcgatg
     8281 tcgctgtcca gcggcaagcc catgacgcag atcacgtcgg cccgcgcctg cgcctacccg
     8341 gggtgtgaga cgaagctttc gcgctacaac cccaatccgt gctgtgcggt gcacggcggc
     8401 tgggccgacg aggaaggtgg ccgccgcagc cggaactcgg tgtgaccggg gggtcgtcac
     8461 ccggcacgtc cggtgcccga acggacaagg agcctgtcgt ggacctcggc ggagcgatgt
     8521 tcggcggact cgccgtgctc gtcgtgctgg tgctggcggc ggtgtacgtg ggggcgcgcc
     8581 tgcacggcgg caaggcgccg tcccacgcgc gggacaacga gcacgccggc cgctctcccg
     8641 ccggcgacga ggaccccgtg cccagggact ccgacggcat gccgatggtg acgccgccgg
     8701 aggacccggc ggagacgtcc gtggacgaga tgcagcggcg agcacgaagg aacgcctcat
     8761 gaccgacgcc cagcgcttcc gcgccctcct ggaggaggag cgcgaccggc tccgcgccat
     8821 ccgcggcggc gcggacggcc acctgagcga cagccaggag gcctccaccg gcgagctcac
     8881 caccttcgac cagcatcccg gggacgtggc ctccgagacg ctggagcgag agcaggacga
     8941 gtccatccgg gagcacgccg aggcgcagct ggaggaggtc gaggcggcgc tgcggcgcct
     9001 ggacgacggc tcgtacgggc gctgcgcggt gtgcggcgcc gacatcggcg aggcgcgcct
     9061 gcaggtgcgg ccgcagacgc gctactgcgt ggagcaccag cacgagcggg agcgcacccg
     9121 gggggcggaa cggcagccgc gggcctagac ccgagcgggc cggtcagagc tcgcagccgt
     9181 gcgcggtccc gcccaggcac gtgtcggtgc cgtcgccgcc gatgagcacg ttcgtcccca
     9241 ggccgccgtt gagggtgtcg ttgccgccac cgcccatgag gcagtcgtcg ccgccgcccc
     9301 cgtcgatgga ctcgccggca tcacgctgag cgcggtgctg
//
LOCUS       DATMKC010000024         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_260383, whole genome shotgun sequence.
ACCESSION   DATMKC010000024 DATMKC010000000
VERSION     DATMKC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260383"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..2347)
                     /locus_tag="VNU01_00750"
     CDS             complement(83..2347)
                     /locus_tag="VNU01_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWH31176.1"
                     /translation="MSSRPPSPRRPSQPPRRPPQRRAAARPPKRGPRAQRPWWKRPIA
                     LLLVLPAAVLGFLALVLFSYTFARVPLPAEISTGATVLLDQAGREIGELTAHTSRQDV
                     PLATLPQHVRHAVLAAEDAGFYDHPGVSVAGIVRAAFRNVVARGVAQGGSTISQQYIK
                     NVTDQRQQTALRKIREAALAMKLEREFSKDQILEFYLNTIYFGRGAYGIQAASQAYFG
                     KAAAELSLPEAAQLAGMIPAPSALDPAKNPERAHQRYRYVLERMAVNEWLPEAEAAAL
                     AAAPPAVLEPRPVQFRTAPFFLDMVQRELAGRLGEDMVYAGLRVTTTLDLGIQEVAER
                     EFNEALADAPDEEAAAAMVSVDPATGGIRALVNGRPGEQINFVADSRRQPGSTFKPFA
                     LAAWVEEGNSPESTFEGPAQMTFDNPGSEPYEVNNYGRQGFGRISLREATWKSVNTVY
                     AQLTEEVGPAAVVDIAHRAGVDSELEPNLSLTLGTNGVTPLELAGAFNTFAAGGVRRT
                     PFTVLKVERDGQTLYQARPGDDRAFSDQVAWTVTDVLRGVLQAGTGRAADIGRPAAGK
                     TGTTQNSADAWFAGYTPHLTTVVWYGQRDGNSGLPSEPTGGGWPARLWGAFMGEALQG
                     VEAADFPEPRGDLRVVGPTAEPSPEATEEPEPLECPEGQVPVTAEDEDGGEADELQQV
                     CAPSPSESPSPTQSPSPTQTPSPTQTQSPTASPSPTTTEPEPTATADAGGGGSGDGVQ
                     TSGETSEPSPSPSG"
BASE COUNT          265 a          877 c          889 g          334 t
ORIGIN      
        1 ggctgagacg acctcgggcg gggcgtccgc gtcgtcggtg gcgggccgcc ccgaccgcgc
       61 ggacgccccg cccgaggtcg tctcagcctg acggcgacgg gctgggctcg ctggtctccc
      121 ccgacgtctg cacgccgtcg cctgagccgc ccccgccggc gtcggcggtg gcggtcggtt
      181 ccggctcggt ggtggtgggc gaggggctcg ccgtggggct ctgcgtctgc gtgggcgacg
      241 gcgtctgcgt gggcgacggc gactgcgtgg gggacggcga ctccgagggc gacggggcgc
      301 acacctgctg gagctcgtcg gcctcgccgc cgtcctcgtc ctccgcggtg acggggacct
      361 ggccctccgg gcactccagc ggctcgggct cctcggtggc ctccgggctc ggctcggccg
      421 tcgggccgac cacgcgcagg tcgccccggg gctcggggaa gtcggcggcc tccacccctt
      481 gcagcgcctc gcccatgaac gcgccccaca gccgcgccgg ccagccgcct cccgtgggct
      541 cgctgggcag gcccgagttg ccgtcgcgct ggccgtacca gaccaccgtg gtcaggtgcg
      601 gggtgtagcc cgcgaaccac gcgtccgcgg agttctgggt ggtgccggtc ttgcccgccg
      661 ccgggcggcc gatgtcggcg gcacggccgg tgccggcctg cagcacgccc ctcagcacgt
      721 cggtcaccgt ccacgccacc tggtcggaga acgcccggtc gtccccgggc cgcgcctggt
      781 acagggtctg cccgtcgcgc tcgaccttca gcacggtgaa cggcgtgcgc cgcacgccgc
      841 cggcggcgaa cgtgttgaac gcgccggcca gctccagcgg tgtcaccccg ttggtgccca
      901 gcgtcagcga caggttcggc tccagctcgc tgtccacgcc ggcgcggtgc gcgatgtcca
      961 cgaccgcggc gggcccgacc tcctcggtca actgcgcgta caccgtgttg accgacttcc
     1021 acgtcgcctc ccgcagcgag atgcgcccga agccctggcg cccgtagttg ttgacctcgt
     1081 acggctcgct gccggggttg tcgaacgtca tctgcgccgg tccctcgaag gtgctctccg
     1141 gggagttgcc ctcctccacc caggcggcca gggcgaacgg cttgaacgtg gagcccggct
     1201 ggcggcggct gtcggccacg aagttgatct gctcgccggg ccggccgttg accagcgccc
     1261 ggatgccgcc ggtggccggg tcgaccgaca ccatcgcggc ggccgcctcc tcgtcgggcg
     1321 cgtcggcgag cgcctcgttg aactcccgct cggcgacctc ctggatgccc aggtcgagcg
     1381 tcgtggtgac gcgcagcccg gcgtacacca tgtcctcgcc cagcctgccg gccagttcgc
     1441 gctgcaccat gtccaggaag aacggggcgg tgcggaactg caccggccgc ggctcgagca
     1501 cggccggggg ggccgccgcc agggccgccg cctcggcctc gggcagccac tcgttgacgg
     1561 ccatgcgctc cagcacgtag cggtagcgct ggtgcgcccg ctcggggttc ttcgccgggt
     1621 ccagggcgct gggcgccggg atcatgccgg cgagctgcgc cgcctcggga agcgacagct
     1681 cggccgccgc cttgccgaag taggcctggg aagccgcctg gatgccgtag gcgccgcggc
     1741 cgaagtagat ggtgttcagg tagaactcca ggatctggtc cttggagaac tcccgctcca
     1801 gcttcatcgc gagcgcggcc tcgcggatct tgcgcagcgc ggtctgctgg cgctggtcgg
     1861 tcacgttctt gatgtactgc tggctgatcg tggagccgcc ctgggcgacg ccgcgcgcga
     1921 cgacgttgcg gaacgcggcg cgcacgatgc ccgcgacgct cacgcccggg tggtcgtaga
     1981 agccggcgtc ctcggcggcc aggaccgcgt gccgcacgtg ctggggcagc gtggccagcg
     2041 gcacgtcctg gcggctggtg tgcgcggtca gctcgccgat ctcccgtccg gcctggtcca
     2101 gcaggaccgt ggcgccggtg ctgatctccg cgggcagggg cacgcgcgcg aacgtgtagg
     2161 agaacagcac cagagccaga aagcccagga ccgccgcggg cagcaccagc agcagcgcga
     2221 tcggccgctt ccaccagggg cgctgcgctc gcgggccccg tttgggcggg cgcgccgccg
     2281 cgcgccgctg cggcggacga cgagggggct gcgagggacg ccgcggggac ggcggacgcg
     2341 aactcatgat cgcccagtct gccac
//
LOCUS       DATMKC010000025         4310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_259813, whole genome shotgun sequence.
ACCESSION   DATMKC010000025 DATMKC010000000
VERSION     DATMKC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259813"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VNU01_00755"
     CDS             <1..204
                     /locus_tag="VNU01_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31177.1"
                     /translation="VAAAVEGVEVYDVAPLDATARVRALRDAVEQALPGHLDSHRCCA
                     GHVADLAFDLPDLLAPAQVDAEV"
     gene            234..1400
                     /locus_tag="VNU01_00760"
     CDS             234..1400
                     /locus_tag="VNU01_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HWH31178.1"
                     /translation="MSHPSALIWSDDRLRYDFGPDHPLKPVRVELTVALIRACGLTDR
                     DDVRTLPPDPFGDDDVVRLHRPDYVEAVKRLSAAPDPAGDGRYGLGLGDNPVFAGMHE
                     ASLEVCGASVAAARAVWEGQVAHAFNPAGGLHHAMPERAAGFCIYDDPAFAIDWLLAH
                     GAERVAYVDIDTHHGDGVQAMFYDDPRVLTISLHESGRYLFPGTGFTHEIGEGDGRGA
                     SLNVPLPPTTPGQVWLDAFERVVPEALDGFAPDVLVTQLGCDTHVTDPLAHLALVTDD
                     YRVVYRRLHELAHEHAAGRWVAMGGGGYQVATVVPRAWTLAFAELSGGTLPERLPPEW
                     LREALRRTGEMAPDVFTDGEVRLTDERVALVRGEALRTVDELKALAWPLLERLG"
     gene            1405..2160
                     /locus_tag="VNU01_00765"
     CDS             1405..2160
                     /locus_tag="VNU01_00765"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HWH31179.1"
                     /translation="MDLRAHLESTRLAGAVATTPGSTVENCGRLVAGDERYTFGLTDW
                     RTASLDEAVEAVRRVCGGDPAGAPAGSPGCIDLDATLGGIERHRRALAAAAGGGAARV
                     LLATGHPTGLLAHYGAIGRALQAHGCHLLAPLDDRMLWPADDGRPRGVRFIDGVAAVT
                     DGGKLLHTHRSRYMEAMLDALDGRADLVVADHGMAGAAIERGIATLSIADVNDPALLL
                     AQSRGRTADVLVIDDNLAPRVFVPVTRAMLDWR"
     gene            2157..3197
                     /locus_tag="VNU01_00770"
     CDS             2157..3197
                     /locus_tag="VNU01_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH31180.1"
                     /translation="MIRVPVRLGGVLAVLLGMALAVPAAAQTPSSEPSAQPQRHSARI
                     VRVSGDSRVATAVAASRHAFPGGAGTVVIAAARRFPDALAAVPLAAREGAPLLLVEER
                     ARGAVLEEVRRLGASRALLMAAHGAVPVVVELELRRAGLVVERVAGDGRFHTASRIAR
                     RLGPGEAGEAVLVSGEAFADALPAGPLAAHAGLPILLTAAGELPADPAAALRDLPAER
                     VLVVGGPAAVGEAVVAGLPDARRVSGPDRYRTALAAADELLARGGSLGTVAIATGRDF
                     ADALAAGPVAAQAGGPVLLVDGQDAAQADAVYDYLAEHRGDIDTVLLFGGAAAISEDV
                     RGRIARALGGDA"
     gene            3194..3733
                     /locus_tag="VNU01_00775"
     CDS             3194..3733
                     /locus_tag="VNU01_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HWH31181.1"
                     /translation="MSSIGACAALLLAGVFAWAGIAKLRAPGAAAFSFAGLGLPRPRA
                     LAAVVPWAELALAAALVWQPRLGSLAALLLLGAFSVALARARAGGARTGCACFGGGRA
                     DAPLSGALLRNALLAMAALAGLGGQRGWVDVPALVAVGTAAVVGVVALALWDLRVRAG
                     SLWSGGALADARAGSEGSA"
     gene            3730..4230
                     /locus_tag="VNU01_00780"
     CDS             3730..4230
                     /locus_tag="VNU01_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWH31182.1"
                     /translation="MSGTVWVVSYVLLWLAVVVLGVAVVVLLRQIGVLHTRLRPLGVH
                     HADRGPVRGDLAPPAGVDYAASEHTLVAFTAADCPVCHELLPSLRVLARDYPEVRLHQ
                     VDMGPATERVFAAFRVDLTPYLVLVGRDGTVLGGGVANTLEQAEVLIESARGPAAAPA
                     SAEGRP"
BASE COUNT          435 a         1601 c         1706 g          568 t
ORIGIN      
        1 gtcgcggccg ccgtggaggg cgtcgaggtc tacgacgtcg ccccgctgga cgccaccgca
       61 cgcgtgcgag cgctgcgcga tgccgtggag caagctctgc ccgggcacct ggacagccac
      121 cgctgctgcg ccgggcacgt cgccgacctc gccttcgacc tgccggacct gctggcgccg
      181 gcgcaggtgg acgccgaggt ctgaccggcg cgggagggtc tagtctcccc gccatgtccc
      241 atccgagcgc gctgatctgg tccgacgacc ggctgcgcta cgacttcggc cccgaccacc
      301 cgctcaagcc ggtgcgcgtg gaactgaccg tggccctcat ccgggcgtgc ggcctcaccg
      361 accgcgacga cgtgcgcacg ctgccccccg atcccttcgg tgacgacgac gtcgtgcgcc
      421 tgcaccgtcc cgactacgtg gaggcggtga agcgtctgtc ggccgcgccc gatcccgccg
      481 gtgacggccg ctacggcctg ggcctgggcg acaacccggt gttcgcgggc atgcacgagg
      541 cctcgctgga ggtgtgcggc gcctccgtgg ccgccgcccg cgcggtctgg gaggggcagg
      601 tcgcccacgc gttcaacccg gccggcggac tgcaccacgc catgcccgaa cgggccgccg
      661 ggttctgcat ctacgacgat cccgccttcg ccatcgactg gctgctggcg cacggcgccg
      721 agcgcgtcgc ctacgtcgac atcgacacgc accacggcga cggcgtgcag gcgatgttct
      781 acgacgaccc ccgggtgctg acgatcagcc tgcacgagtc cggccgctac ctgttcccgg
      841 gtaccggctt cacccacgag atcggcgagg gcgacggccg cggcgccagc ctcaacgtcc
      901 cgctgccgcc cacgacgccg ggacaggtgt ggctggacgc gttcgagcgc gtggtgccgg
      961 aggccctgga cggcttcgcc ccggacgtgc tggtgaccca gctcggctgt gacacgcacg
     1021 tgacggaccc gctcgcgcat ctggcgctgg tgaccgacga ctaccgggtg gtctaccggc
     1081 gcctgcacga gctggcgcac gagcacgccg ccggccgctg ggtcgccatg ggcggcggcg
     1141 gctaccaggt cgccacggtg gtgccgcgcg cgtggaccct ggcgttcgcc gagctgagcg
     1201 gcggcacgct gcccgagcgc ctgccgcccg agtggctgcg ggaggccctg cgccgcaccg
     1261 gcgagatggc ccccgacgtg ttcaccgacg gcgaggtgcg cctgaccgac gagcgggtcg
     1321 cgctggtgcg gggcgaggcg ctgcgcaccg tcgacgagct caaggcgctg gcctggccgc
     1381 tcctggaacg cctgggctga ccgcatggac ctgcgcgcgc atctggagtc gacccgtctg
     1441 gccggtgcgg tcgcgaccac ccccggcagc accgtggaga actgcggcag gctcgtcgcc
     1501 ggcgacgagc gctacacgtt cggcctgacc gactggcgca cggccagctt ggacgaggcg
     1561 gtcgaagcgg tccgccgcgt ctgcggcggc gaccccgccg gcgcgccggc cggctcgccc
     1621 ggctgcatcg acctcgacgc cacgctgggc ggcatcgagc gccaccggcg cgcgctggcc
     1681 gcggccgccg gcggcggcgc cgcgcgggtg ctgctggcca ccgggcaccc cacggggctg
     1741 ctggcgcact acggcgccat cggtagggcg ctccaggcgc acggctgtca cctgctggca
     1801 ccgctcgacg accgcatgct gtggcccgcc gacgacgggc ggccgcgcgg cgtgcgtttc
     1861 atcgacggtg tggcggccgt gacggacggc ggcaagctgc tgcacaccca tcgcagccgc
     1921 tacatggagg cgatgctgga cgccctcgat ggccgggcgg acctcgtggt ggcggatcac
     1981 ggcatggccg gcgccgcgat cgagcgcggc atcgccacgc tgtccatcgc ggacgtgaac
     2041 gacccggcgc tgctgctggc ccagtcgcgc gggcgcaccg cggatgtgct ggtcatcgac
     2101 gacaacttgg cgccgcgcgt tttcgtcccg gtcacccggg cgatgctgga ctggcgatga
     2161 tccgagtgcc tgtccggctc ggaggcgtgc tcgcggtgct gctcgggatg gcgctggccg
     2221 tgccggcggc ggcgcagacc ccctccagcg agccttccgc ccagccgcaa cggcacagcg
     2281 cccgcatcgt gcgcgtctcc ggcgacagcc gggtcgccac ggccgtggcc gcgtcccgcc
     2341 acgcattccc gggcggcgcc ggcacggtgg tcatcgcggc ggcgcggcgg ttccccgatg
     2401 cgctggcggc cgtgccgctg gcggcccgcg agggcgcacc gctgctgctg gtggaggaac
     2461 gcgcgcgcgg cgccgtcctg gaggaggtcc ggcggctggg ggcgagccgc gcgctgctca
     2521 tggccgcgca cggcgcggtg ccggtggtcg tggaactgga gttgcggcgc gcgggcctgg
     2581 tggtcgagcg ggtggccggt gacgggcgct tccacaccgc ctcgcgcatc gcccgccggc
     2641 tcggcccggg cgaggcgggc gaagccgtgc tcgtgtccgg ggaggccttc gccgacgcac
     2701 tgccggccgg tccgctcgcc gcgcacgcgg gcctgccgat cctgctgacc gcggccggcg
     2761 agctgcccgc ggaccccgcg gccgcgctgc gggacctgcc ggccgagcgg gtcctggtgg
     2821 tcggcggccc ggccgcggtc ggcgaagcgg tggtggcggg cctgccggac gcccggcgcg
     2881 tctccgggcc ggaccgctac cgcacggcgc tcgcggcggc ggacgagctg ctggcccggg
     2941 gcggcagcct gggcacggtg gcgatcgcca ccggccgcga cttcgccgac gccctggccg
     3001 cggggcccgt cgcggcgcag gccgggggcc cggtgctgct ggtcgacggg caggacgcgg
     3061 cgcaggcgga cgcggtctac gactacctgg ccgagcaccg cggcgacatc gacaccgtgc
     3121 tgctgttcgg cggggcggcg gcgatcagcg aggacgtgcg cggcaggatc gcccgcgcgc
     3181 tgggagggga cgcgtgagca gcatcggcgc gtgcgcggcg ctcctgctcg ccggcgtgtt
     3241 cgcctgggcc ggcatcgcca aactgcgcgc gcccggggcg gcggcgttct ccttcgccgg
     3301 gctcggcctg ccgcggccgc gcgcgctggc cgccgtggtg ccgtgggcgg aactggcgct
     3361 ggccgcggcg ctggtgtggc agccgcggct cggctcgctc gcggcgctgc tcttgctggg
     3421 cgcgttcagc gtggcgctgg cccgcgcccg cgccggcggt gcgcgcacgg gctgcgcctg
     3481 cttcggcggc gggcgcgcgg acgcgccgct gtccggcgcg ctgctgcgca acgccctgct
     3541 cgcgatggcg gcgctcgccg ggctcggagg acagcgcggc tgggtggacg tgccggcgct
     3601 cgtcgcggtg ggcaccgcgg ccgtcgtggg cgtcgtcgcg ctggcgctgt gggacctgcg
     3661 ggtgcgagcg ggctcgctgt ggtccggcgg cgccctggcg gacgcccggg cgggatcgga
     3721 ggggagcgca tgagcgggac ggtgtgggtg gtgtcgtacg tgctgctgtg gcttgccgtc
     3781 gtggtgctcg gcgtggccgt cgtggtgctg ctgcgccaga tcggggtcct gcacaccagg
     3841 ctgcgcccgc tcggggtgca ccacgccgac cgggggcccg tgcgcgggga cctggcgcca
     3901 ccggccggcg ttgactacgc cgcgtccgag cacactctgg tcgccttcac cgccgcggac
     3961 tgcccggtct gccacgagtt gctgccgtcc ctgcgcgtgt tggcgcgcga ttacccggag
     4021 gtgcgcctgc accaggtgga catggggccg gccacggagc gcgtcttcgc cgccttccgc
     4081 gtggacctga cgccctacct ggtcctggtc ggccgtgacg ggaccgtgct cggcggcggc
     4141 gtggccaaca cgctggaaca agccgaggta ctgatcgagt ccgcgcgtgg cccggcggcc
     4201 gcgcccgcgt cggcggaggg ccggccgtga gcgcggtcga caccgtggtg gagcggctcg
     4261 gccgcggtct ggcggcccgc acctcgcggc gcggattcct ggcgcaggcc
//
LOCUS       DATMKC010000026         7702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_259621, whole genome shotgun sequence.
ACCESSION   DATMKC010000026 DATMKC010000000
VERSION     DATMKC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259621"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VNU01_00785"
     CDS             <1..692
                     /locus_tag="VNU01_00785"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HWH31183.1"
                     /translation="SVAVLAMSPRGTMFDPGPMVYMMKWVVGPDAGGTIDIDAPIGDN
                     LRKIAKAKGKDVSDLTVIMLDRERHAEYMREVRESGARLRLIMDGDVAASLLAAMPEK
                     NVDVLIGIGGTPEGVTTACAIRALGGEMVGRLWARNDEEVVAAKEQGFDLDEVLTTER
                     LVSSPDTFFVCTGITDGDLVAGVNYFRGGATTESIVMRGKSGTIRYVKARHNLDKVME
                     YSSIDFDPGRS"
     gene            complement(790..1743)
                     /locus_tag="VNU01_00790"
     CDS             complement(790..1743)
                     /locus_tag="VNU01_00790"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH31184.1"
                     /translation="MSDAAPEQTELDPGVIWPKDRRVRTDDGAELAYTFLGPADGPVV
                     ALCSGFLCPDTWWAELAPTLADAGYRVLVFHYRGVASSTLPREPGWHGRGLGREALSI
                     ERFAADLRTIADHEGIAELAVAGHSMGSQVALEAYRQMPGRVRAIVSLTGPYASPVRT
                     LFGFRRAGVALYAATLAAFLLTPPPVLRTVWRQAWRLPIPQMAKLMGVVAPRCPNERL
                     DTFVDHGAWVDPYVAMATTSGMHAHSAEDLLPEIDVPVLVVVGGVDPFSPPSLGRHMA
                     ERIPDATLRTVPRGSHGALLEYPELVNGWVLDFLDANRARR"
     gene            1997..2977
                     /locus_tag="VNU01_00795"
     CDS             1997..2977
                     /locus_tag="VNU01_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWH31185.1"
                     /translation="MRLNKLLLGLLMALALVATACGNAPDDTGTTDNEEAPEDGGGPA
                     GDEVSGTIEIDGSSTVAPLTDAIAEEYNAAQPGVEVNVGVSGTGGGFERFCETGDTQI
                     SNASRPIKESEAQACADNGIEFTEVRVGTDALTMVTSADTDWVDCLTTEEVVKLWGPD
                     GVDSWDQVREGFPAEPVQVFAPGADSGTYDFMNETVLEEDVQPRQDYNASEDDNVIAQ
                     GIQGTKGSWGFFGFAYYQENADTMKAISFDAGDGCVEPSAETAQDDSYKLARPLFIYV
                     RNDALQEAHVADFVTFYLDTVNDVIEEVGYIPATDEELAEARASVEGAIG"
     gene            2995..3972
                     /gene="pstC"
                     /locus_tag="VNU01_00800"
     CDS             2995..3972
                     /gene="pstC"
                     /locus_tag="VNU01_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWH31186.1"
                     /translation="MSEHPVIEAPVLVSGPGDPGQTSLAGRPRRGEKLVMVLLALAGA
                     VSVLTTVGIVAVLFFDAIEFFRASSVSVSEFFLGTEWRPFGDPENEAFRVGVLPLVNG
                     TLMVTGIAMLIAVPLGLASAMYLSEYAPARVRSVLKPSLELLAGVPTVVLGYFALTFM
                     TPLLRSLFGTDNVSIFNAASAGIVMGIMIVPTIASLSEDAMSAVPRGLREAAYGLGAG
                     KRHVVLKIVTPAALSGIVAAVILGLGRAIGETMIVAIAAGNLPQMTADPFAQIQTMTA
                     YIVQAVKGEAARGSLTYQSIFAVGGLLFVMTFTLNILAQRFVRRFREVY"
     gene            3972..4880
                     /gene="pstA"
                     /locus_tag="VNU01_00805"
     CDS             3972..4880
                     /gene="pstA"
                     /locus_tag="VNU01_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWH31187.1"
                     /translation="MGLGTPASSVETAVQALATPKRRGARERVFAVVLLSATVVSVLV
                     LGVLIADVLMDGLGRINGEFFTSYASRRAERTGIRAGLTGSLSLLVMVAAMAFPLGVA
                     AALYLEEFAPDNRLTRVLEANIANLAGVPSVVYGLLGAAVFVYLFEFGRSLLTGAFTL
                     ALLILPVVIVASREALRAVPRAIRDGGLALGATPLQVAWRQTVPSALPGILTGTILAL
                     SRAVGETAPILVAGAVLFRRSDNVPWGFFDAFTALPIQIYDFVSRPQEAFRVEAAAAA
                     IIVLLAVLLVMNSAAIVLRNRYARRW"
     gene            4880..5767
                     /gene="pstB"
                     /locus_tag="VNU01_00810"
     CDS             4880..5767
                     /gene="pstB"
                     /locus_tag="VNU01_00810"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWH31188.1"
                     /translation="MPWTPAEASVRPPAPAPEGRTSPTAAHHGVSVDAQRVFELENVS
                     VAYGGNIAVRDVTMPIHRNEITALIGPSGCGKSTLLRCLNRMNDLIPGARVDGAVRYH
                     GINLYDKGVDPVEVRRRIGMVFQKPNPFPKSIYENIAFGPKVLGMKDNLDDRVETCLR
                     RAALWEEVKQKLHSSAYGLSGGQQQRLCIARAIAVNPDVILMDEPCSALDPIATARIE
                     DLMRELEDEYTIVIVTHNMQQAARVSDRTAFMTTDVEPDEDGKDKRSGRLVEYDLTDK
                     IFKNPGDRRTEDYISGRFG"
     gene            complement(5780..6106)
                     /locus_tag="VNU01_00815"
     CDS             complement(5780..6106)
                     /locus_tag="VNU01_00815"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWH31189.1"
                     /translation="MTAAATQPCCPPIAEPTLSPGDAERAAGVFKALGHPARVTIVNL
                     LARATGAGVCVCDLTEPTGLSQPTVSHHLKLLRQAGLIEGERRGTWIYYRLVPQALSD
                     IQRALA"
     gene            6154..6573
                     /locus_tag="VNU01_00820"
     CDS             6154..6573
                     /locus_tag="VNU01_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31190.1"
                     /translation="MADVLFVCVHNAGRSRMAEALFNHWSRERYGGRWTAASAGTQPA
                     PRPHPEVVEAMAELGIAVEVRPGELLTPEMAEGARKLFSMGCNVEEACPALTIPMEDW
                     ALEDPKGRPMPEVRTIRDDIARRVGHLLEQLESGLGG"
     gene            complement(6556..7242)
                     /gene="phoU"
                     /locus_tag="VNU01_00825"
     CDS             complement(6556..7242)
                     /gene="phoU"
                     /locus_tag="VNU01_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWH31191.1"
                     /translation="MSGELRAGHREHLVQLEDRVLAMGDRVLDMADQAMDALERRDLE
                     LGRAVIAMDDALDREYQAVQDGILTTLALQAPVASELRLVAALLHVNVHLERMGDVCV
                     NIAKFVKLLGDFPGEDELLRQIHEMGEHGKRVARRALEAFSSRDLEAAREVPLLDDPL
                     DQLNKGLFRRLVDLAASDETRLDWAMRMVLVARNLERFGDHAVDIAEQAVFAVTGDMV
                     ELSSNSPTQS"
     gene            7329..>7702
                     /locus_tag="VNU01_00830"
     CDS             7329..>7702
                     /locus_tag="VNU01_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH31192.1"
                     /translation="MPKLMIVEDEPPLREALVYALESEGFAVVACDRGDTAVERFERE
                     RPDLVLLDVMLPGLSGVEVCKRIRLTHATPIIMLTARDTEIDTVVGLEVGADDYVTKP
                     FSVRELVARIRAVLRRGGEWDQ"
BASE COUNT         1150 a         2706 c         2741 g         1105 t
ORIGIN      
        1 gttccgtggc ggtgctggcc atgtcgccgc gcgggaccat gttcgacccg ggtcccatgg
       61 tctacatgat gaagtgggtg gtgggcccgg acgccggcgg cacgatcgac atcgacgcgc
      121 ccatcggcga caacctgcgc aagatcgcca aggccaaggg caaggacgtc agcgacctca
      181 cggtcatcat gctggaccgc gaacgccacg ccgagtacat gcgcgaggtg cgcgagagcg
      241 gcgcgcgcct gcgcctgatc atggacggcg acgttgccgc cagcctgctc gccgccatgc
      301 cggagaagaa cgtcgacgtg ctcatcggca tcggcggcac gccggagggc gtcaccacgg
      361 cgtgcgcgat ccgcgcgctg ggcggggaga tggtgggccg cctgtgggcg cgcaacgacg
      421 aggaagtcgt cgccgccaag gagcagggct tcgacctgga cgaggtgctc accacggagc
      481 gcctggtgtc cagccccgac acgttcttcg tctgcaccgg catcaccgac ggcgacctgg
      541 tcgccggggt gaactacttc cgcggcggcg ccaccacgga gtccatcgtc atgcgcggca
      601 agtccggcac catccgctac gtcaaggcgc gccacaacct ggacaaggtg atggagtaca
      661 gctcgatcga cttcgacccc ggccgctcct gacccgccgg acgccggctg ccgccgatgt
      721 ggaagaacgc gtattgggtc gcatcgggtg caaggacgca ccggatccga cccaatacgg
      781 acttgtccac tagcgtcgag cccgattcgc gtcgaggaag tccaggaccc agccgttgac
      841 cagctcgggg tactccagca gcgcgccgtg gctgccgcgc gggacggtcc gcagcgtcgc
      901 gtcggggatg cgctcggcca tgtggcggcc gaggctcggc ggcgagaacg ggtccacccc
      961 gccgaccacc accagcacgg gcacgtcgat ctccggcagc aggtcctccg cactgtgcgc
     1021 gtgcatgccg gacgtcgtgg ccatcgccac gtacgggtcc acccacgcgc cgtggtccac
     1081 gaacgtgtcc aggcgctcgt tggggcagcg cggcgccacc acgcccatga gcttggccat
     1141 ctgcgggatg ggcagccgcc acgcctgcct ccacaccgtg cgcagcaccg gcggcggggt
     1201 cagcaggaac gccgccagcg tcgccgcgta cagcgccacg ccggcgcggc ggaagccgaa
     1261 cagcgtgcgc accggcgagg cgtacggtcc ggtcagcgac acgatcgcgc ggacccggcc
     1321 gggcatctgg cggtacgcct ccagggcgac ctgcgagccc atcgagtgcc cggccaccgc
     1381 cagctcggcg atgccctcgt ggtcggcgat cgtgcgcagg tccgcggcga aacgctcgat
     1441 cgacagcgcc tcccgcccga ggcccctgcc gtgccagccc ggctcgcgcg ggagcgtgga
     1501 gctcgccacg ccgcggtagt ggaacaccag gacgcggtag ccggcgtcgg ccagcgtggg
     1561 cgcgagctcg gcccaccagg tgtccgggca caggaagccc gagcacaggg ccaccacggg
     1621 accgtcggcg ggacccagga aggtgtacgc cagctccgcg ccgtcgtcgg tccgcacgcg
     1681 gcggtccttc ggccagatga cccccgggtc cagctcggtc tgctcgggcg cggcgtcgct
     1741 catcgcctga tggtgccgtt ccggcgccca ggcccgcaaa agccggtgtg ttcacccgcc
     1801 gttcacctgc gcgtcagcga gtcgtcagcc cgccgggtta tgacgtgtgc gtgcacgggc
     1861 agcagaaggg aaatgtcggt cagcggccac cggccgttca cctggcagac cgagcggcgt
     1921 caccggacga acctactgtc gaggtcgttg acacccacag tctggtcgga acacgccagt
     1981 gaggagagag atcccgatgc gtctgaacaa gcttctgctg ggcctgctca tggccctcgc
     2041 cctggtcgcc acggcatgcg ggaacgcgcc ggacgacacc ggcaccaccg acaacgagga
     2101 agcgccggag gacggcggcg ggcccgcggg cgacgaggtc agcgggacca tcgagatcga
     2161 tggctcgtcc acggtcgccc cgctgaccga cgccatcgcc gaggagtaca acgccgccca
     2221 gccgggcgtc gaggtgaacg tgggcgtgtc cggcacgggc ggcggcttcg agcgcttctg
     2281 cgagaccggc gacacccaga tctccaacgc gtcccgcccc atcaaggaat ccgaggcgca
     2341 ggcctgcgcc gacaacggca tcgagttcac cgaggtgcgc gtcggcactg acgcgctcac
     2401 catggtcacc agcgcggaca ccgactgggt cgactgcctc accaccgagg aggtcgtcaa
     2461 gctctggggc ccggatggcg tggactcctg ggaccaggtc cgggagggct tcccggccga
     2521 gcccgtccag gtgttcgccc ccggtgccga ctcgggcacg tacgacttca tgaacgagac
     2581 ggtcctggag gaggacgtcc agccgcggca ggactacaac gccagcgagg acgacaacgt
     2641 catcgcccag ggcatccagg ggaccaaggg ctcgtggggc ttcttcggct tcgcctacta
     2701 ccaggagaac gccgacacca tgaaggcgat ctccttcgac gccggcgacg gctgcgtgga
     2761 accgtccgcc gagaccgcgc aggacgactc ctacaagctc gcccgccccc tgttcatcta
     2821 cgtgcgcaac gacgcgctgc aagaggcgca cgtggccgac ttcgtgacct tctacctgga
     2881 cacggtcaat gacgtgatcg aggaggtcgg ctacatcccc gcgaccgacg aggagctggc
     2941 ggaggcccgc gcctccgtcg agggcgcgat cggctagcgc caactgggag gaccatgagc
     3001 gagcatcccg tcatcgaggc gcccgtgctg gtgtccggac cgggcgatcc cggccagacg
     3061 tccctggccg gccggcctcg tcgcggcgag aagctcgtca tggtcctttt ggccctggcc
     3121 ggcgccgtgt cggtgctcac caccgtgggc atcgtcgcgg tgctgttctt cgacgcgatc
     3181 gaattcttcc gcgcgtcctc ggtgagcgtg tcggagttct tcctcggcac ggagtggcgg
     3241 cccttcgggg acccggagaa cgaggccttc cgcgtcggcg tgctgccgct ggtgaacggc
     3301 acgctcatgg tcacggggat cgcgatgctg atcgcggtgc cgctgggcct cgcgtcggcg
     3361 atgtacctgt cggagtacgc gccggcgcgg gtccgctcgg tgctcaagcc gtcgctggag
     3421 ctcctggcag gcgtgccgac cgtcgtgctg ggctacttcg ccctgacgtt catgaccccg
     3481 ctgctgcggt cgctgttcgg caccgacaac gtgtccatct tcaacgccgc cagcgcgggg
     3541 atcgtgatgg ggatcatgat cgtgccgacc atcgcgtcgc tgtcggagga cgccatgagc
     3601 gcggtgccgc gcggcctgcg cgaggcggcg tacgggctgg gtgcgggcaa gcgccacgtg
     3661 gtcctcaaga tcgtcacgcc cgcggcgctg tccggcatcg tcgccgcggt catcctgggc
     3721 ctcgggcgcg ccatcggcga gaccatgatc gtcgccatcg cggccgggaa cctgccccag
     3781 atgaccgccg acccgttcgc ccagatccag accatgacgg cctacatcgt gcaggcggtg
     3841 aagggcgagg cggcccgcgg ctcgctcacc taccagtcga tcttcgccgt gggcgggctg
     3901 cttttcgtga tgacgttcac cctgaacatc ctggcccagc gcttcgtccg ccgcttccgg
     3961 gaggtctact gatggggctc ggcacgccgg ccagcagcgt ggagacggcc gtccaggcgc
     4021 tcgcgacgcc gaagcgtcgc ggcgcgcgcg agcgcgtctt cgccgtggtg ctgctgtccg
     4081 cgaccgtcgt gtccgtgctg gtgctgggcg tgctgatcgc cgacgtgctc atggacgggc
     4141 tgggccgcat caacggcgag ttcttcacca gctacgcctc tcggcgcgcg gagcgcacgg
     4201 ggatccgcgc cggcctgacg ggaagcctgt cgctgctggt gatggtggcc gccatggcgt
     4261 tcccgctcgg cgtcgcggcc gccctgtacc tggaggagtt cgcccccgac aaccggctga
     4321 cgcgcgtcct cgaggccaac atcgcgaacc tggcgggcgt gcccagcgtg gtctacgggc
     4381 tgctgggcgc ggcggtcttc gtctacctgt tcgagttcgg ccgcagcctg ctgacgggcg
     4441 cgttcacact cgccctgctg atcctgccgg tggtcatcgt ggcgtcgcgc gaggccctgc
     4501 gcgcggtgcc ccgggcgatc cgggacggcg gcttggccct gggcgccacg ccgctgcagg
     4561 tcgcctggcg ccagaccgtg ccgtcggcgc tgcccggcat cctcaccggc accatcctgg
     4621 ccctgtccag ggcggtcggc gagacggcgc ccatcctggt ggccggcgcc gtgctgttcc
     4681 gccgcagcga caacgtgccg tggggcttct tcgacgcgtt cacggcgctg ccgatccaga
     4741 tctacgactt cgtcagccgc ccgcaggaag cgttccgcgt ggaggccgcc gcggcggcga
     4801 tcatcgtcct gctggccgtg ctgctcgtca tgaactccgc ggcgatcgtg ctgcgcaacc
     4861 gctacgcaag gagatggtga tgccctggac tccggctgag gcgagcgtgc gcccccccgc
     4921 gcccgcaccg gaagggcgga cgtccccgac ggccgcgcac cacggcgtgt cggtggacgc
     4981 gcagcgggtc ttcgagctgg agaacgtgag cgtggcctac ggcggcaaca tcgccgtccg
     5041 tgacgtgacc atgccgatcc accgcaacga gatcaccgcg ctgatcgggc cgtcaggctg
     5101 cggcaagtcg acgctgctgc gctgcctcaa ccgcatgaac gacctcattc ccggggcgcg
     5161 cgtggacggc gcagtgcggt atcacgggat caacctgtac gacaagggtg tggaccccgt
     5221 cgaggtgcgc cggcgcatcg gcatggtgtt ccagaagccc aacccgttcc ccaagtcgat
     5281 ctacgagaac atcgccttcg gccccaaggt gctgggcatg aaggacaacc tcgacgaccg
     5341 ggtggagacc tgcctgcgcc gcgcggcgct gtgggaagag gtcaagcaga agctgcactc
     5401 gagcgcctac ggcctgtcag gcgggcagca gcagcgcctg tgcatcgcgc gcgccatcgc
     5461 cgtgaacccc gacgtgatcc tcatggacga gccgtgctcg gcgctggacc cgatcgccac
     5521 cgcgcgcatc gaggacctga tgcgggagct ggaggacgag tacaccatcg tcatcgtcac
     5581 ccacaacatg cagcaggcgg ctcgcgtcag cgaccgcacg gcgttcatga ccaccgacgt
     5641 ggagcccgac gaggacggca aggacaagcg gtcgggccgg ctggtggagt acgacctcac
     5701 ggacaagatc ttcaagaacc ccggcgaccg gcgcacggag gactacatca gcgggcgctt
     5761 cggctaggac gcgcccgggt cacgccaggg cgcgctggat gtcgctcagg gcctgcggga
     5821 cgaggcggta gtagatccag gtgccgcgcc gctcgccctc gatgaggccg gcctggcgga
     5881 ggagcttgag gtgatgcgac acggtgggct gcgacaggcc ggtcggttcg gtcaggtcgc
     5941 acacgcacac acccgcgccg gtggcgcgcg cgagcaggtt cacgatggtc acgcgcgcgg
     6001 ggtggccgag ggccttgaag acgccggcgg cgcgctcggc gtcgcccggc gacagcgtcg
     6061 gctcggcgat cggcgggcag cagggctggg tggccgcggc ggtcatgggc ccatggtggc
     6121 acaagggcgg ccatgccgcc ggggaggcga ctgatggctg acgtgctgtt cgtctgcgtc
     6181 cacaacgcgg ggcgctcccg catggccgag gcgctgttca accactggtc gcgggagcgc
     6241 tacggcggcc gctggaccgc cgccagcgcc ggcacgcagc cggcgccgcg cccccacccc
     6301 gaggtggtgg aggcgatggc cgagctgggc atcgccgtgg aggtccgccc cggcgagctg
     6361 ctcacccccg agatggccga gggcgcccgc aagctgttct ccatgggctg caacgtcgag
     6421 gaggcctgcc cggcgctcac catcccgatg gaggactggg cactggagga cccgaagggc
     6481 cggcccatgc ccgaggtccg caccatccgc gacgacatcg cccgccgcgt ggggcacctg
     6541 ctcgagcaac tggagtcagg actgggtggg tgagttgctc gacagctcca ccatgtcgcc
     6601 ggtgaccgcg aacaccgcct gctcggcgat gtccaccgcg tggtcgccga agcgctccag
     6661 gttgcgggcc accaagacca tgcgcatcgc ccagtccaga cgcgtctcgt ccgacgcggc
     6721 gaggtcgacc agccgccgga acaggccctt gttgagctgg tccagcgggt cgtccagcag
     6781 cggcacctcg cgggccgcct ccaggtcgcg gctggagaac gcctccagcg cccggcgggc
     6841 gacgcgcttg ccgtgctcgc ccatctcgtg gatctggcgc aacagctcgt cctcgccggg
     6901 gaagtcgccc aggagcttca cgaacttggc gatgttcacg cagacgtcgc ccatgcgctc
     6961 caggtgcacg ttcacgtgca gcagcgcggc gaccagccgc agctcgctgg ccacgggagc
     7021 ctgcagcgcc agggtggtga ggatgccgtc ctggaccgcc tggtactcgc ggtccagcgc
     7081 gtcatccatg gcgatgacgg cgcggccgag ctcgaggtcg cgccgctcca gcgcgtccat
     7141 cgcctggtcc gccatgtcca ggacgcgatc gcccatggcg aggacgcggt cctccagctg
     7201 gaccaggtgc tcccggtggc cggcgcgcag ttcaccgctc atcgcggaca aggactccct
     7261 tcgtggccga tggtcagcat cataggcaca gcctccccgt tcggggcggc tcctagactg
     7321 gaacgcgcat gcccaagctc atgatcgtcg aggacgagcc gccgctgcgg gaggccctcg
     7381 tgtacgcgct ggagtcggag ggcttcgcgg tggtcgcctg cgaccgcggc gacaccgcgg
     7441 tggagcgctt cgagcgcgag cggcccgacc tggtgctgct ggacgtgatg ctgccggggc
     7501 tgtccggtgt ggaggtgtgc aagcgcatcc gcctgacgca cgccacgccg atcatcatgc
     7561 tgacggcgcg cgacaccgag atcgacacgg tcgtcggcct ggaggtcggc gcggacgact
     7621 acgtcaccaa gccgttcagc gtgcgcgagc tggtggcgcg catccgggcg gtcctgcgcc
     7681 gcgggggcga gtgggaccag tt
//
LOCUS       DATMKC010000027         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_259320, whole genome shotgun sequence.
ACCESSION   DATMKC010000027 DATMKC010000000
VERSION     DATMKC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259320"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="ftsY"
                     /locus_tag="VNU01_00835"
     CDS             complement(<1..1006)
                     /gene="ftsY"
                     /locus_tag="VNU01_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWH31193.1"
                     /translation="MEILILGIAVLVLVALMVGLVALPRRRQAPREPALDEQARDEAA
                     TPTPLVPERLLEEPAEDVVAVPDAGAVAEERPLSVRDRFRLRLGRARGGLGAGIAGAF
                     RRGVDEQAWEELEEALISADVGVEAALEIVSGLRDRAKAEGVRTGEEALALLKEVLRL
                     ELSVADRTLARRAGGPSVWLVTGVNGTGKTTSIGKLAARYRREGQEVVLAAADTFRAA
                     AAEQLQHWGERSGARVVRHGPGADPAAVAFDGWQAAKSAGADLLVVDTAGRLQNKSEL
                     MAELSKVKRVLERDAGQLDEVLLVLDATTGQNGLSQARAFLEAVDVSGVVLTKLDGTA
                     K"
     gene            1047..1607
                     /locus_tag="VNU01_00840"
     CDS             1047..1607
                     /locus_tag="VNU01_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWH31194.1"
                     /translation="MDLPFDIPPTLVRRELAAYLGDAARPEAQVLPTRCPPWTVADVT
                     AHVAETFARFNRMLAQSRAGDLTPPFTPKELAEENLRRVAAFAGDPVEALEREATAFV
                     DAVADQDEPIAHQFGPLPLRVQLGFALNELAIHHDDVAHAQRRAYEPSPETTALLAQL
                     WTSLGGLPIEEPADTDWKRILRASGR"
     gene            complement(1604..>2370)
                     /locus_tag="VNU01_00845"
     CDS             complement(1604..>2370)
                     /locus_tag="VNU01_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31195.1"
                     /translation="GVAARLHEDYEIDAQAALAERRDSDPESLLPDAERDAELAEQED
                     KLLKKVALLGTVNPLALQEFEAMQERHAFLEGQIADLRQSKKDLMEVVEKVDERIKEV
                     FAAAFADVAREFERIFPRMFPGGEGKLVLTDPDNLLDTGVEVEARPPGKRVKRLSLLS
                     GGERSLTALAVLFAIFSARPSPFYVLDEVEAALDDVNLQRFLDVVKDFKQASQMLIVT
                     HQKRTMEVADCLYGITMQGNGVSKTISQKVSESVSA"
BASE COUNT          307 a          915 c          827 g          321 t
ORIGIN      
        1 ccttcgcggt gccatcgagc ttggtcagca ccacgccgct gacgtcgacg gcctccaaga
       61 acgccctggc ctgcgacagg ccgttctgcc cggtggtggc gtccaggacc agcagcacct
      121 cgtcgagctg cccggcgtcg cgctccagca cgcgcttgac cttggacagc tcggccatga
      181 gctcgctctt gttctgcagc cgcccggcgg tgtcgaccac cagcaggtcg gccccggcgg
      241 acttggcggc ctgccagccg tcgaacgcca ccgcggcggg gtccgcccca gggccgtgac
      301 gcacgacgcg cgcgccggag cgctcgcccc agtgctggag ctgctcggcg gccgcggcgc
      361 ggaacgtgtc ggcggccgcg agcacgacct cctggccctc gcggcggtag cgcgcggcca
      421 gcttgccgat ggaggtggtc ttgccggtgc cgttgacgcc ggtgaccagc cagacgctcg
      481 gcccgccggc gcgccgcgcc aaggtgcggt cggcgacgga cagctccagg cgcaggacct
      541 ccttgagcag cgccagcgcc tcctcgccgg tgcggacgcc ttcggccttg gcgcggtcgc
      601 gcaggccgct gacgatctcc aaggcggcct ccacgccgac gtcggcggag atcagcgcct
      661 cctccagctc ctcccaggcc tgctcgtcca cgccccgccg gaacgcgccg gcgatcccgg
      721 cgcccaggcc gccgcgcgcg cggcccagcc gcaggcggaa gcggtcgcgc acggacagcg
      781 gtcgctcctc ggcgacggcg ccggcgtccg ggaccgccac gacgtcctcc gccggctcct
      841 ccagcagccg ctcgggcacc aggggcgtcg gcgtggcggc ctcgtcgcgc gcctgctcgt
      901 ccagcgccgg ctcgcggggc gcctgccggc ggcggggcag ggcgacgagg ccgaccatca
      961 gcgcgaccag cacgagcacg gcgatgccga gaatcaggat ctccatgggc ggcagcctac
     1021 ccgcgtggcc gcgtagcctc cctggcgtgg acctgccgtt cgacatcccg ccgacgctgg
     1081 tgcgccgcga gctggcggcc tacctcggcg acgccgcccg acccgaggcc caggtcctgc
     1141 cgacgcgctg cccgccgtgg acggtcgcgg acgtgaccgc gcatgtggcg gagacgttcg
     1201 cgcgcttcaa ccgcatgctg gcgcagagcc gcgcgggcga cctcaccccg ccgttcacgc
     1261 cgaaggagct ggccgaggag aacctgcgcc gcgtggcggc gttcgccggc gacccggtgg
     1321 aggcgctgga gcgcgaggcc acggcgttcg tggacgcggt ggccgaccag gacgagccga
     1381 tcgcgcacca gttcgggccg ctgccgctgc gcgtgcagct gggcttcgcg ctgaacgagc
     1441 tggcgatcca ccacgacgac gtcgcccacg cccagcgccg ggcctacgag ccgtcgcccg
     1501 agaccaccgc gctgctcgcg caactgtgga cctcgctggg cggcctgccg atcgaggagc
     1561 ccgccgacac cgactggaag cgcatcctgc gcgcctccgg ccgctaggcg gagacggact
     1621 cggagacctt ctgggagatg gtcttcgaca cgccgttgcc ctgcatggtg atgccgtaca
     1681 ggcagtccgc cacctccatc gtgcgcttct ggtgcgtcac gatgagcatc tggctggcct
     1741 gcttgaagtc cttcaccacg tccaggaagc gctggaggtt cacgtcgtcc agcgcggcct
     1801 ccacctcgtc cagcacgtag aacggcgacg gcctcgcgga gaagatcgcg aacagcacgg
     1861 ccagcgccgt cagggagcgc tcgccgccgg acagcaggga caggcgcttc acgcgcttgc
     1921 cggggggccg cgcctccacc tccacgccgg tgtcgaggag gttgtccggg tcggtcagca
     1981 cgagcttgcc ctcgccaccc gggaacatcc gcgggaagat gcgctcgaac tccctcgcca
     2041 cgtcggcgaa cgcggccgcg aagacctcct tgatgcgctc gtcgaccttc tcgaccacct
     2101 ccatgaggtc cttcttggac tggcgcaggt cggcgatctg gccctccagg aacgcgtggc
     2161 gctcctgcat cgcctcgaac tcctgcagcg ccagcgggtt caccgtgccg agcagcgcga
     2221 ccttcttgag cagcttgtcc tcctgctcgg ccaactcggc gtcgcgctcg gcgtccggca
     2281 gcagcgactc cgggtcgctg tcgcggcgct ccgccagggc cgcctgcgcg tcgatctcgt
     2341 agtcctcgtg caggcgcgcg gccacgccat
//
LOCUS       DATMKC010000028         4235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_259018, whole genome shotgun sequence.
ACCESSION   DATMKC010000028 DATMKC010000000
VERSION     DATMKC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259018"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VNU01_00850"
     CDS             complement(<1..1006)
                     /locus_tag="VNU01_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH31196.1"
                     /translation="MLPTDRAVPTGAALGRRGAIALLAVAGLFATQLSAAAAPSARGS
                     AWTSAALDPGLSVVGEALQDVIVTAAGGVEAAADAVRSIGGTVESPLPIVEGVEARVP
                     ANRLPELAGVTGVTGITADRRASFEELSYDDSTAVASNFVKTTQANAAWSGGNTGAGV
                     GIAVLDTGISEMNDFKGRLVHGPDLSGEGTLVDTYGHGTVMAGAAGGSGLDSVGSNKG
                     IYAGVAPKSTLVAVKVAGRNGVADVSTILQGMHWVSAYKHQFNIRVMNLSWGTKSTQD
                     PKVDPLNYAVQRLWQLGIVVVVAAGNSGPGAGTIMKPADDPLVVSVGAYDEKGNTSTS
                     G"
     gene            complement(1175..2032)
                     /locus_tag="VNU01_00855"
     CDS             complement(1175..2032)
                     /locus_tag="VNU01_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWH31197.1"
                     /translation="MWQELALPGLIDLHVHFMPKGVMDKVWAYFDAVGPLTGISWPIA
                     YRTEEQDRLARLRAMGVLAFPSLVYPHKPDMATWLNSWAAGFAAAEPDVLRTATFHPE
                     PGAAAYVRTAIEEGAQVFKAHLQVGAYDPRDPLLDEVWGQLAEAAVPVVVHCGSGPTP
                     GPFTGPGPFGEVLARHPRLTAVVAHMGMPEYEDFLAMAERYPRVHLDTTMAFTDFVEA
                     RSPFPPGLRSRLADLADRVLLGSDFPNTPYPYAHQVEALLRLDLGDYWLRRVLHDNAD
                     GLIPRAHAA"
     gene            complement(2059..2589)
                     /gene="smpB"
                     /locus_tag="VNU01_00860"
     CDS             complement(2059..2589)
                     /gene="smpB"
                     /locus_tag="VNU01_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWH31198.1"
                     /translation="MTKGPAKTRNPGKGPPDGARGGRNKLVAQNKKARHDYSIEDVVE
                     AGLVLTGTEVKSLRQGRCSLADAYATVDAGEIYLHGAHIPEYTEGTWNNHAPRRARKL
                     LLHKREIESLAMRTREGGLALVPLSLYFKDGRAKVELALGRGRKAHDKRQVLAERDAQ
                     KEMRNAMGRALKRGRR"
     gene            complement(2599..3933)
                     /locus_tag="VNU01_00865"
     CDS             complement(2599..3933)
                     /locus_tag="VNU01_00865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH31199.1"
                     /translation="MRLRRVAALALLPVAGLPFLLTPAPSAAAVQVAAPRTQSLEELR
                     LREREAAGDLENSSQQVQAAGAALRAVEQQLPGARRAVAEAKGELAGARAKAAAATAE
                     VRRAEAEAAAAAREVERASSKVEEGRAEVGRLARRTYQRGRLSDLRDVMEAGEPQDAL
                     LRAGMLRSVFRTQNDSLSRLTADRLALAGTRADLAADERALEQARAEAERVAARAREV
                     AVRAEQAARKVEDLAAQRKAVLAQAEKHRAEDLQDYREAQAASAALAERIRKAAAKAA
                     AERAAAERRRKAAEEAERKKRERAGQKPSRSEPRSDRMLWPAPGRLTSRYGYRTHPIY
                     GDRRFHAGIDIGGGLGARVSASDGGYVTYAGYASGYGTLVVISHGTVGGRDLSTAYAH
                     MGELTVREGQTVTKGQKVGEIGNEGNSTGPHLHFEVRLNGDPVDPLDYVDPP"
     gene            complement(4003..>4235)
                     /locus_tag="VNU01_00870"
     CDS             complement(4003..>4235)
                     /locus_tag="VNU01_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31200.1"
                     /translation="FLLEGVLAATVGAALASGALVALKAVLIDGQLRPNFPVIAYVSW
                     ADVLWILPWLFVAGIVLAGLSSFITLQRHLRV"
BASE COUNT          523 a         1589 c         1536 g          587 t
ORIGIN      
        1 acccgctggt ggacgtgttg cccttctcgt cgtaggcgcc cacggagacc accagcgggt
       61 cgtcggccgg cttcatgatg gtgccggcac ccgggccgga gttgcccgcg gcgacgacga
      121 cgacgatccc gagctgccac aggcgctgga cggcgtagtt gagcgggtcg accttcgggt
      181 cctgggtgct cttcgtgccc caggacaggt tcatgacccg gatgttgaac tggtgcttgt
      241 aggccgacac ccagtgcatt ccctgcagga tcgtcgagac gtctgcgacg ccgttgcgcc
      301 ccgcgacctt gacggcgacc agcgtcgact tcggcgccac gccggcgtag atgcccttgt
      361 tggaccccac cgagtcgagc ccgctgccgc cggccgcgcc ggccatgacg gtgccgtggc
      421 cgtaggtgtc gacgagcgtg ccctcaccgg acaggtccgg tccgtgcacc agccggccct
      481 tgaagtcgtt catctccgag atgccggtgt ccagcaccgc gatgccgacg ccggcgccgg
      541 tgttgccgcc gctccaggcc gcgttcgcct gcgtcgtctt cacgaagttg ctggcgaccg
      601 ccgtcgagtc gtcgtaggag agctcctcga agctggcccg ccggtcggcc gtgatgccgg
      661 tgacgccggt gacgccggcc agctcaggca gccggttggc cgggacccga gcctcgacgc
      721 cctcgacgat cgggagcggg gactccaccg tgccgccgat cgagcggacc gcgtccgcgg
      781 ctgcctcgac gccccccgca gccgtcacga tgacgtcctg cagcgcctcg ccgaccacgg
      841 acaggcccgg gtcgagcgcg gccgacgtcc aggcgctgcc gcgcgccgac ggggcggccg
      901 cggccgacag ctgggtggcg aacaggccgg cgacggccag cagcgcgatc gcaccgcggc
      961 ggcccagcgc agcgcccgtc gggacggcgc ggtcggtggg gagcatgctc acctcaggag
     1021 atgggggtgg gagaagtacc tcacgcatgg tggccgagga gtcgctgtct gtgcatgacc
     1081 gatccgggcc gtcggcggct agtcacttcg gactagacgg cctgcctccg ctacagaagt
     1141 gcgggcgcaa gccgggacat cgcgcccacg ttcgtcacgc agcgtgagcg cgtggaatca
     1201 ggccgtcagc gttgtcgtgc agcacccggc ggagccagta gtcaccgagg tcgagccgga
     1261 gcagcgcctc cacctggtgg gcgtacgggt agggcgtgtt cgggaagtcg ctgcccagca
     1321 gcacccgatc ggccaggtcg gccagccgcg accggagccc gggcgggaag ggcgaccgcg
     1381 cttccacgaa gtcggtgaag gccatggtgg tgtccaggtg cacccgcggg tagcgctcgg
     1441 ccatcgccag gaagtcctcg tactccggca tccccatgtg cgccacgacg gcggtgagcc
     1501 gcgggtgccg ggccagcacc tcgccgaagg gcccgggccc ggtgaacggc ccgggcgtgg
     1561 ggccgctgcc gcagtggacg acgaccggga ccgcggcctc ggccagctgc ccccacacct
     1621 cgtccagcag cgggtcgcgc gggtcgtacg cccccacctg caggtgcgcc ttgaagacct
     1681 gcgcgccctc ctcgatcgcc gtgcggacgt acgccgcggc acccggctcg gggtggaagg
     1741 tggccgtgcg tagcacgtcg ggctcggcgg cggcgaagcc ggccgcccac gagttcaacc
     1801 aggtcgccat gtccggcttg tgggggtaga ccagcgacgg gaaggccagc acgcccatcg
     1861 cccgcagccg cgccagccgg tcctgctcct cagtgcggta ggcgatcggc caggagatgc
     1921 cggtcagcgg ccccaccgcg tcgaagtagg cccacacctt gtccatcacg cccttgggca
     1981 tgaagtgcac gtgcaggtcg atcagccccg ggagagcgag ctcctgccac accgcgggga
     2041 cctgctcgtc ggtgagcgtc agcgccggcc cctcttgagc gcccggccca tcgcgttgcg
     2101 catctccttc tgcgcgtcgc gctcggccag cacctgccgc ttgtcgtgcg ccttgcgccc
     2161 gcggccgaga gcgagctcga ccttggcccg gccgtccttg aagtagagcg agagcggcac
     2221 cagcgccagg ccgccctccc gggtgcgcat cgcgagggac tcgatctccc gcttgtgcag
     2281 cagcagcttg cgggcccgcc gcggcgcgtg gttgttccag gtcccctcgg tgtactccgg
     2341 gatgtgcgcg ccgtgcaggt agatctcgcc cgcgtccacg gtggcgtacg cgtcggccag
     2401 cgagcaccgg ccctgtcgca gcgacttgac ctcggtgccg gtcagcacca gccccgcctc
     2461 gaccacgtcc tcgatcgagt agtcgtgccg ggccttcttg ttctgggcca ccagcttgtt
     2521 gcggccgccc cgcgcgccgt cgggcggacc cttgcccggg ttgcgcgtct tcgccgggcc
     2581 cttggtcacc gggcgagtct agggcgggtc gacgtagtcg agcgggtcga ccgggtcgcc
     2641 gttcaggcgc acctcgaagt gcaggtgcgg cccggtggag ttgccctcgt tgccgatctc
     2701 gccgaccttc tggcccttgg tgaccgtctg gccctcgcgc accgtcagct cgcccatgtg
     2761 cgcgtacgcc gtggacaggt cccgaccccc cacggtcccg tggctgatca cgaccagcgt
     2821 cccgtagccg ctggcgtagc cggcgtaggt gacgtagccg ccgtcggagg cgctcacgcg
     2881 cgccccgagc ccgccgccga tgtcgatgcc ggcgtggaag cggcggtcgc cgtagatcgg
     2941 atgggtgcgg tagccgtagc ggctcgtcag gcggcccggg gccggccaca gcatccggtc
     3001 cgagcgaggc tcgctgcggg agggcttctg gccggcgcgc tcgcgcttct tgcgctcggc
     3061 ctcctccgcc gccttgcgcc gccgctcggc cgccgcccgc tcggcggcgg ccttggccgc
     3121 ggccttgcgg atccgctcgg cgagcgccgc ggaggcggcc tgcgcctccc ggtagtcctg
     3181 caggtcctcg gcgcggtgct tctcggcctg ggccagcact gccttgcgct gcgccgcgag
     3241 gtcctcgacc ttcctggccg cctgctcggc ccggacggcc acctcacggg cacgcgccgc
     3301 gacgcgctcg gcctcggccc gggcctgctc gagcgcccgc tcgtcggcgg ccaggtcggc
     3361 gcgggtgccg gccagggcca gccggtctgc cgtcagccgg ctgaggctgt cgttctgcgt
     3421 gcggaagacc gagcggagca tccctgcgcg cagcagcgcg tcctgcggct cgccggcctc
     3481 catcacgtcg cgcaggtccg acagccggcc gcgctggtag gtgcgccggg cgagccggcc
     3541 cacctccgca cggccctcct ccaccttgct gctggcccgc tcgacctcgc gcgcggcggc
     3601 ggcggcctcg gcctcagccc ggcgcacctc cgcggtcgcg gcggcggcct tggcgcgcgc
     3661 ccccgcgagc tcacccttgg cctcggccac ggcccgccgg gcgccgggca gctgctgctc
     3721 caccgcgcgc agcgccgccc cggccgcctg cacctgctgg ctggagttct cgaggtcccc
     3781 cgcggcctcg cgctcgcgca gcctcagctc ctccagcgac tgggtgcgcg gcgccgccac
     3841 ctggacggcc gcggcggagg gcgccggcgt cagcaggaac ggcaggccgg cgaccgggag
     3901 gagggccagc gcggccaccc ggcgcaggcg cacgggcagg cgccctgggg aagcagggac
     3961 gtccacgcag tcacatctac cacgtgggcc ccaggaatcc cgtcagacgc gcaggtgccg
     4021 ctgcaacgtg atgaagctgg acaagccggc cagcacgatg ccggcgacga acagccacgg
     4081 caggatccac agcacgtcgg cccagctgac gtaggcgatg accgggaagt tcgggcgcag
     4141 ctggccgtcg atgagcacgg ccttgagcgc gaccagcgca ccggaggcca gggccgcgcc
     4201 gaccgtcgcg gcgagcacgc cctcgagcag gaacg
//
LOCUS       DATMKC010000029         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_17421, whole genome shotgun sequence.
ACCESSION   DATMKC010000029 DATMKC010000000
VERSION     DATMKC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17421"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VNU01_00875"
     CDS             <1..204
                     /locus_tag="VNU01_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31201.1"
                     /translation="KQTFSGEVEIDGALNHDGTTLGVLGATPVTQRTHIADPSGGITS
                     DSQARTAINAILDLLEAYGLMAP"
     gene            221..511
                     /locus_tag="VNU01_00880"
     CDS             221..511
                     /locus_tag="VNU01_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31202.1"
                     /translation="MKVARITGRVAFGTGRSVRLKAGDQFPDDDPIVKARPELFESPE
                     SYVARTRKPRTTAELGSRSMSARKMIAEQAEVEQATAAPGEKRDVKRAKREG"
     gene            516..1943
                     /locus_tag="VNU01_00885"
     CDS             516..1943
                     /locus_tag="VNU01_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31203.1"
                     /translation="MSAGTGKVVVAYLHPGEVSHSFMQSMMKLWLYEQPKGRILTATA
                     QECGSARIIEGRNDAVRTFLRTEAEWLLFIDADMGFAPYALEQLLEVADPDERPIVGA
                     LCFGQRKGPETGAHARHFRAVPTLYQWSESPGQAGFAPMYGYRDLFPANAVVKVDGTG
                     SAFILIHRRVFETIRDATTPEGAPAFPAPREWYADTLYKGSIIGEDLTFCLRAQQFGF
                     PVYVHTGVKTSHYKHVYLDESTVGDLTDAPNFVVIPTKGEGPVYDLCRTLADQGEAAG
                     IFVMDNGMPESKRKLVEGVPGVEVVDAKGRNIHQMWNAGLAEARARAFPANVAVLNDD
                     IKVGPAFLSGLARPLRTHQMLAAVCPNYDGRDLKPDQATAVADICAGRYDGTGGLAGF
                     AFMLKAEDGYRFPEELQWWWGDNDLLLSIHAAGMEAAIVGSATCEHIDGGGKTGKWDA
                     PEMQTVLAADRAAFEAKWRPALEAA"
     gene            1944..2423
                     /locus_tag="VNU01_00890"
     CDS             1944..2423
                     /locus_tag="VNU01_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31204.1"
                     /translation="MHGQVIDFVSSCPREFATVVEIGSRDINGTVRGLFTAESYTGID
                     LYEGPGVDVVGDCLDWSPPEPVDAVVCCEVLEHAPDAEGIVRRAHEWLRPGGTLILTC
                     ATDPRSAHSGISEQPWKPGEFYRNVKPDDMRSWLDGFDVRRLEVGPPGDLRVLAVKE"
     gene            2426..>2987
                     /locus_tag="VNU01_00895"
     CDS             2426..>2987
                     /locus_tag="VNU01_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31205.1"
                     /translation="MSDTLDILTLAEGKTACNITGTGHDTELAQFITAVSRRIDELCG
                     PVVNRTVTDELHNGGRGFIRPRKTPVDSVTTLVEYRHTTDTSLTEETNATKQADGFMV
                     DEEWAHNVRIIRRTSGSDAAFPAGRRNVVLTYVAGRAANTAAVDAKFKRAAGDMLQRM
                     WRRGAAAWSGGGDPFEANAVQHNRLFL"
BASE COUNT          488 a          995 c         1085 g          419 t
ORIGIN      
        1 aaacagacgt tctcgggcga agtcgagatc gacggcgccc tgaaccacga cggcacgacg
       61 ctcggcgtgc tcggcgccac cccggtgacg cagcgcacgc acatcgcgga cccgtcgggc
      121 gggatcacgt cggattcgca ggccaggacc gcaatcaacg cgattctgga cctgctcgaa
      181 gcctatgggc tcatggcccc gtaacaggaa ggcggacagc atgaaggttg cacggatcac
      241 cggaagggtc gcgttcggca cgggccgctc ggtgcggctc aaggcggggg accagttccc
      301 cgacgacgac ccgatcgtga aggcgcggcc ggagctgttc gagtcgccgg agtcctacgt
      361 ggcgcgcacg cgcaagccgc gcaccacggc cgagctcggc tcgcgttcca tgagcgcccg
      421 caagatgatc gcggagcagg ccgaggtgga gcaggcgact gcggcgccag gcgagaagcg
      481 cgacgtcaag cgcgccaagc gcgagggctg agccggtgtc ggcgggcacc ggcaaggtgg
      541 ttgtggccta cctgcatccc ggggaggtct cccacagctt catgcagtcg atgatgaagc
      601 tgtggctgta cgagcagccg aagggccgca tcctcaccgc gacggcgcag gagtgcgggt
      661 cggcgcggat catcgagggc cgcaacgacg cggtgcgcac gttcctgcgc actgaggccg
      721 agtggctgct gttcatcgac gccgacatgg gatttgcccc gtacgcgctg gagcaactgc
      781 tggaggtggc ggaccccgac gagcgcccca tcgtgggggc gttgtgcttc gggcagcgca
      841 aggggccgga gacgggcgcg cacgcccgcc acttccgtgc ggtgccgacg ctgtaccagt
      901 ggtccgagtc gcccgggcag gcggggttcg cgccgatgta cggctaccgg gacctgttcc
      961 cggccaacgc cgtggtgaag gtggacggca ccggctcggc gttcatcctg atccaccgcc
     1021 gggtgttcga gacgatccgc gacgccacca caccggaggg cgcgccggcg ttccccgctc
     1081 cgcgcgagtg gtacgccgac acgctctaca agggctcgat catcggggag gacctgacct
     1141 tctgtctccg cgcgcagcag ttcgggttcc cggtgtacgt ccacacgggc gtcaagacct
     1201 cccactacaa gcacgtctac ctggacgagt ccacggtggg cgacctgacc gacgcaccga
     1261 acttcgtggt catcccgacc aagggcgagg ggccggtcta cgacctgtgc cgcacgctcg
     1321 cggaccaggg cgaggccgca ggcatcttcg tcatggacaa cggcatgccg gagtccaagc
     1381 gcaagctggt cgagggcgtg ccgggcgtcg aggtggtgga cgccaagggc cgcaacatcc
     1441 atcagatgtg gaacgcgggg cttgccgagg cgcgcgcccg cgccttcccg gcgaacgtcg
     1501 cggtcctgaa cgacgacatc aaggttggcc cggcgttcct gtccgggctg gcccggccgc
     1561 tgcgcacgca ccagatgctc gcggcggtct gcccgaacta cgacgggcgg gacctcaagc
     1621 cggaccaggc cacggcggtg gccgatatct gcgccggccg ctacgacggc acgggaggcc
     1681 tcgcgggctt cgcgttcatg ctcaaggccg aggacggcta ccgcttccct gaggaacttc
     1741 agtggtggtg gggcgacaac gatctgctgc tgtccatcca cgccgccggc atggaggcgg
     1801 ccattgtcgg ctccgcgacg tgcgagcaca tcgacggcgg cggcaagacc ggcaagtggg
     1861 acgcgccgga gatgcagacg gtgctcgcgg ccgaccgcgc agcgttcgag gccaagtggc
     1921 gcccggcgct ggaagcggcc tagatgcacg gccaggtgat cgacttcgtg tcgtcgtgcc
     1981 cgcgcgagtt cgccacggtc gtggagatcg gctcccgcga catcaacggc accgtccgtg
     2041 gcctgttcac cgccgagtcc tacaccggga tcgacctgta cgaaggccct ggggtggacg
     2101 tggtgggcga ctgcctggac tggtcgccgc cggaaccggt ggacgcggtg gtgtgctgcg
     2161 aggtgctgga gcacgcgccc gacgccgagg ggatcgtccg ccgggcgcac gagtggctgc
     2221 ggccgggcgg gacgttgatc ttgacgtgtg cgaccgaccc gcgttcggcg cactcgggga
     2281 tctctgagca gccgtggaag cccggcgagt tctaccgcaa cgtcaagccc gacgacatgc
     2341 ggtcgtggct ggacggcttc gacgtgcggc gcctggaggt cggcccaccc ggtgacctac
     2401 gggtcctggc cgtgaaggag tagccgtgag cgacacgctc gacatcctga ccttggccga
     2461 gggcaagacc gcgtgcaaca tcaccggcac cggccacgac accgagctcg cccagttcat
     2521 tacggccgtc tcgcggcgca tcgatgagct gtgcgggccg gtggtgaacc gcaccgtcac
     2581 cgatgagttg cacaacggcg gccgagggtt catccggccg cgcaagactc cggtggactc
     2641 ggtcaccacg ctggtggagt accgccacac cacggacacc tcgctgaccg aggagaccaa
     2701 cgccaccaag caggctgacg gcttcatggt ggacgaggaa tgggcgcaca acgtccgcat
     2761 catccggcgc accagcgggt ctgacgcggc gtttcccgca ggcaggcgca acgtcgtgct
     2821 gacctacgtg gccgggcgcg cggcgaacac ggcggcggtc gatgcgaagt tcaagcgggc
     2881 ggccggggac atgctccagc ggatgtggcg ccgcggcgcc gcggcctggt cgggcggcgg
     2941 ggatccgttc gaggccaacg ccgtccagca caaccgcctg ttcctgg
//
LOCUS       DATMKC010000030         4473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_258571, whole genome shotgun sequence.
ACCESSION   DATMKC010000030 DATMKC010000000
VERSION     DATMKC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258571"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VNU01_00900"
     CDS             <1..592
                     /locus_tag="VNU01_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31206.1"
                     /translation="ACAARAAKQAADTQAAAKAAADKKARAEAARAAKAEAEKVAADK
                     KAKAAADKKAKAAADKKAKAAADKKAQAAADKKVKAAADKKAEAARQAQVEAQARQAE
                     QAEQAEQAEQAAAQAAAQAAEAARVAAAPSGPPTLRVVGAEDAPVRRGPKEPPAAART
                     APARAAGQRASVPGWADVLLSTAPVARSTDPGPDEG"
     gene            616..1317
                     /locus_tag="VNU01_00905"
     CDS             616..1317
                     /locus_tag="VNU01_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31207.1"
                     /translation="MAVLTLLAGCEAPTRTVRVPVGPAPAELTALDRAVADVDAARTA
                     LLAAPAAVVSGATAFDAGDEACATGDRARAAEAREAARAAGRQVEAALATYADQLAAY
                     RSTLRTLVDAAAPLDPAQREALAALAQAGERDAAALAAFGDAARAAWPAYTALGQVQS
                     TWLDRASAGWYRSPVEAADAYVVLRRPAVPGLEAARTTLARADSARRPATERVRAALA
                     AANRALDALRAPPSR"
     gene            1383..2756
                     /locus_tag="VNU01_00910"
     CDS             1383..2756
                     /locus_tag="VNU01_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31208.1"
                     /translation="MLRLPVRLTPAGPRTTRLVASALALQLTAALGAVALVDRPSPRP
                     GPEATARARVAPAGEAQAQAQAVREQQVRQLLAERGGALLRRDRSAFLASVDPAAQDL
                     RRRQAALFDALEEVPLGIWRYALDASSERPPDTLLDRAYGRGRWWAPGVTLEYGLAGF
                     DERPTVAVHHLTFVQRGGRWLLAADDDFAEVGLATPRALWDRGPVVTERVDDVLVLGR
                     PSARRLLRNVATLTAAAVPRVDDVWGRDWRRGAVVVVPTSAGEMRELLGAGNDLSQIA
                     AVATAELTGSAGEFAPTGDRIVVNPDTFSRLGQLGRRVVLTHELTHVATRRATGPAVP
                     AWLAEGFADYIGYQGVDLPLGLSARELQRDVREGRLPTVLPRDGDFDGGNASLAQAYE
                     GSWLAVRLIAQQYGQDALLRFYRRVGAQRDLDAAEAVDRALRTELGTSTPALTRAWRD
                     ALQRDLG"
     gene            2753..3985
                     /locus_tag="VNU01_00915"
     CDS             2753..3985
                     /locus_tag="VNU01_00915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWH31209.1"
                     /translation="MTRRAPALTLVALLLLLGLALATAVPWDPLPGADLRGDPLRDFT
                     AAQVAREVAFHNAVRPSSYGALVLGLVVAGALGLTRAGARIVGAIRGGWVAQVLLGTL
                     ALTALGRLATLPLSARSEVVLRRYGLSTQTWSSWLLAVVKGVLVGAGLTALALVALVA
                     LARRTRRWWAWGAAATAGLVIAGSFAYPLVVEPVFNRFTPLPAGELREDLLALAARDG
                     VPVEEVLVADASRRTTALNAYVSGFGSSRRIVVYDTLLERATDEEVELVVAHELGHAD
                     ERDVLVGTLLGALGAAAAVCALALVLSWTPLLRRAGADGMADPRVVPVVLALVAVTSL
                     LVAPASNLVSRAIEARADVHALDLTGDPRTFIATQKRLALSNLSDLDPHPLAYAFFAS
                     HPSVTERLSLAREWERLR"
     gene            3982..>4473
                     /locus_tag="VNU01_00920"
     CDS             3982..>4473
                     /locus_tag="VNU01_00920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH31210.1"
                     /translation="MRTLVITNDFPPRPGGIQAFVHSLAVRQPADEVVVYAPAWKGAA
                     AFDGGQPFPVVRHPTSLMLPTPDVLRRARDLVAAERCDRVWFGAAAPLGLLAPRLGVA
                     RAVASTHGHEVGWALLPGARQALARIARGVDVVTYLGDYTRTRLARAVPAAKLARLPS
                     GVDT"
BASE COUNT          476 a         1723 c         1763 g          511 t
ORIGIN      
        1 ggcctgcgct gcccgcgcgg ccaagcaggc agccgacacg caggcggcgg ccaaggccgc
       61 ggccgacaag aaggccaggg cggaggcggc gcgcgcggcc aaggccgagg ccgagaaggt
      121 ggcggccgac aagaaggcca aggcggcggc cgacaagaag gccaaggcgg cggcggacaa
      181 gaaggcgaag gcggcggcgg acaagaaggc ccaggcggcg gcggacaaga aggtcaaggc
      241 cgcggcggac aagaaggccg aggcggcgcg gcaggcgcag gtcgaggcgc aggcacgcca
      301 ggccgagcag gccgagcagg ccgagcaggc cgagcaggcc gcagcgcagg cggcagcgca
      361 ggcggccgag gccgcacggg tggccgccgc accgtccggc ccgcccaccc tgcgcgtggt
      421 cggcgccgag gacgcgccgg tgcggcgtgg tcccaaggag ccgcccgcgg ccgcccgcac
      481 cgcaccggcg cgcgcggccg ggcagcgggc gagcgtgccc gggtgggccg acgtgctgct
      541 gtcgacggcg cccgtcgccc gctccaccga ccccggcccg gacgagggct gatccgccgc
      601 ctgctcgtcg cgctcgtggc ggtgctgacg ctgctggcgg gctgcgaggc gccgacgcgg
      661 acggtgcgcg tgccggtcgg gcccgcaccg gccgagctga cggcgctgga ccgggcggtc
      721 gccgacgtgg acgcggcacg cacggcgctc ttggcggcgc cggcggctgt cgtcagcggc
      781 gccactgcgt tcgacgccgg cgacgaggct tgcgccacgg gcgaccgtgc ccgcgccgct
      841 gaggcacgcg aggctgcccg cgcggccggc cgccaggtcg aggcggcgct cgcgacgtac
      901 gccgaccagc tcgcggccta ccgcagcacc ctgcgcacgc tcgtcgacgc cgccgcgccg
      961 ctggacccgg cgcagcggga ggcgctcgcc gcgctggctc aggcggggga gcgggacgcc
     1021 gccgcgctgg cagccttcgg tgacgcggcg cgcgccgcgt ggccggcgta caccgcgctg
     1081 gggcaggtgc agtcgacgtg gctggaccgc gcctcagcgg gctggtaccg cagcccggtg
     1141 gaggcggccg acgcgtatgt cgtgctgcgc cgcccggcgg tgcccgggct ggaggctgcc
     1201 cgcaccacct tggcgcgcgc cgacagcgcc cgccgccccg ccaccgagcg ggtgcgggcc
     1261 gcgctcgctg ccgccaaccg cgcgctggac gcgctgcggg cgccgccgtc ccggtgaccg
     1321 gcaggtgggc cccggcgccg caacgcgccg cgcagggcgc agcgccgccg tacgctcagg
     1381 gggtgctgcg cctgcccgta cggctgacgc cggcgggccc gcggacgacc cgcctggtgg
     1441 cgtcggcgct cgccctccag ctgaccgctg cgctcggcgc cgtcgcgctc gtcgaccggc
     1501 cgtcgccgcg cccgggcccc gaggcgacgg cgcgggcccg cgtcgcgccg gccggggagg
     1561 cgcaggctca ggctcaggcg gtccgggagc agcaggtgcg ccagctgctc gccgagcgcg
     1621 gcggcgcgct gctgcgccgc gacaggtccg ccttcctcgc cagcgtcgac cccgcggcgc
     1681 aggacctgcg gcggcggcag gcggcgctgt tcgacgcgct ggaggaggtg ccgctcggca
     1741 tctggcgcta cgcgctggac gcgtcgagcg agcgaccgcc cgacacgctg ctggaccgcg
     1801 cgtacggtcg gggccgctgg tgggcgcccg gcgtgacgct ggagtacggc ctggccggct
     1861 tcgacgagcg cccgaccgtc gccgtgcacc acctcacctt cgtccagcgc ggcgggcgct
     1921 ggctgctcgc ggccgacgac gacttcgccg aggtgggtct ggcgacgccg cgcgcactgt
     1981 gggaccgggg cccggtcgtg accgagcggg tcgacgacgt cctcgtgctc ggccgcccgt
     2041 cggcgcgccg gctgctgcgc aacgtcgcga cgctcaccgc cgcagccgta ccgcgggtcg
     2101 acgacgtctg gggccgcgac tggcggcgcg gcgccgtcgt cgtcgtgccg acgtcggccg
     2161 gcgagatgcg cgagctgctc ggggcgggca acgacctgtc gcagatcgcg gccgtcgcca
     2221 ccgccgagct caccggcagc gcgggtgagt tcgcccccac cggcgaccgc atcgtggtca
     2281 acccggacac tttcagccgg ctgggccagc tcgggcggcg cgtcgtgctc acgcacgagc
     2341 tcacgcacgt cgcgacccga cgcgccaccg gccccgcggt gccggcgtgg ctggccgagg
     2401 gcttcgccga ctacatcggc taccagggcg tcgacttgcc cttgggcctg tcggcgcgtg
     2461 agctccagcg cgacgtgcgg gagggacggc tgcccacggt cctgccccgc gacggcgact
     2521 tcgacggcgg caacgccagc ctcgcgcagg cctacgaggg gtcctggctc gccgtccgcc
     2581 tgatcgccca gcagtacggg caggacgcgc tgctgcgctt ctaccgcagg gtcggcgccc
     2641 agcgcgacct cgacgcggcg gaggcggtcg accgcgcgct gcgcaccgag ctcggcacct
     2701 cgacgccggc cctgacccgc gcctggcgcg acgcgctgca gcgcgacctc gggtgacccg
     2761 tcgcgccccc gcgctcacgc tggtcgcgct cctgctgctg ctcgggctgg ccctggccac
     2821 ggccgtcccg tgggacccgc tgcccggcgc ggacctgcgc ggtgacccgc tgcgtgactt
     2881 caccgccgcg caggtggcgc gggaggtcgc gttccacaac gccgtccggc cgtcgtcgta
     2941 cggcgcgctc gtcctcgggc tcgtggtcgc cggcgcgctc ggcctgaccc gcgcgggcgc
     3001 acggatcgtg ggcgcgatcc gcggcggctg ggtcgcgcag gtgctgctcg gcacgctcgc
     3061 cctcaccgcg ctcgggcggc tcgcgacgct gcccctatcg gcgcgcagcg aggtcgtgct
     3121 gcggcggtac ggcctgtcga cccagacgtg gagcagctgg ctgctcgccg tcgtgaaggg
     3181 cgtcctcgtc ggcgcggggc tcaccgcgct cgcgctggtc gcgctggtcg cgctggcgcg
     3241 gcggacccgg cgctggtggg cctggggagc ggccgccacc gccgggctgg tgatcgcggg
     3301 ctcgttcgcg tacccgctgg tcgtcgagcc ggtcttcaac cgcttcacgc cgctgccggc
     3361 gggcgagctg cgcgaggacc tgctcgcgct cgccgcgcgc gacggggtcc cggtcgagga
     3421 ggtcctggtc gccgacgcct cccgccggac caccgccctg aacgcgtacg tcagcggctt
     3481 cggcagcagc cgccggatcg tcgtgtacga cacgctgctc gagcgcgcga ccgacgagga
     3541 ggtggagctc gtggtcgcgc acgagctcgg ccacgccgac gagcgcgacg tgctggtggg
     3601 caccctgctc ggtgccctcg gtgcggccgc cgcggtctgc gccctcgccc tggtgctgag
     3661 ctggacgccg ctgctgcggc gcgctggcgc tgacggcatg gccgacccac gggtggtgcc
     3721 ggtcgtgctc gcgctggtcg cggtcacctc gctgctggtc gccccggcca gcaacctggt
     3781 gtcgcgcgcg atcgaggcgc gcgccgacgt ccacgcgctg gacctcacgg gtgatccccg
     3841 caccttcatc gccacccaga agcggctcgc cctgtccaac ctgtccgacc tcgatcctca
     3901 cccgctggcg tacgccttct tcgcgagcca cccgtcggtg acggagcggc tgagcctggc
     3961 ccgggagtgg gagcggctgc ggtgaggacc ctggtcatca ccaacgactt cccgccccgc
     4021 ccaggcggga tccaggcgtt cgtccacagc ctcgcggtgc gccagccggc ggacgaggtc
     4081 gtggtgtacg cgccggcgtg gaagggcgcg gcagccttcg acggcgggca gcccttcccc
     4141 gtcgtgcgcc acccgacgtc gctgatgctc ccgaccccgg acgtcctgcg ccgggcccgc
     4201 gacctggtcg ctgccgagag gtgcgaccgg gtgtggttcg gcgcggcggc accgctcggg
     4261 ctgctcgccc cgcggctggg cgtcgcgcgg gcggtggcat ccacccacgg ccacgaggtg
     4321 gggtgggcgc tgctgcccgg tgcgcggcag gcgctggcac ggatcgcgcg cggcgtggac
     4381 gtcgtgacct acctcggcga ctacacgcgc acccggctgg ctcgcgcggt gccggcggca
     4441 aagctggccc ggctgcccag cggcgtcgac acc
//
LOCUS       DATMKC010000031         3547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_353254, whole genome shotgun sequence.
ACCESSION   DATMKC010000031 DATMKC010000000
VERSION     DATMKC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353254"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1830
                     /locus_tag="VNU01_00925"
     CDS             100..1830
                     /locus_tag="VNU01_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HWH31211.1"
                     /translation="MADGDLVVSDSGGATRLKLPGAPNYGWAVAPRAWSNDGQHFVAL
                     RVDNREIHRVPIVDYTSAIERVTWAAYPKSGTPLPVSELHMVDPAAAVARPVQLDTRD
                     AYVWVLGWRPTKTVSEALVMRMSRTGKRLDLFAIDTAGATRLLAQDERPASFVAALDF
                     STGDWAWQVTALPDDSGFLWMSERDGWRHVYHYGYDGAVKKQVTSGAFPVHRVVHVDP
                     ARRQIHVLASGESARPYDRHVYRVGLDGAGFTALTSAPGLHEPAFSASGDFFADTYSS
                     LTQPHTVELRRGDGSLIKVLGQASTIPLEDVGYRPPEPFRVKAADGTTDLYGVLYHPA
                     DFDRSKRYPIVDYIYAGPFIAVHQMTYMPSSAMHRMSASLAEMGFVVVMLDARGTPGR
                     GKAFQDANYGRIGQIEVPDHVAAIRELARSKPYIDTTRAGIVGHSWGGYFALRGMLTA
                     PDFFKAGYAGAPGDLTEAASINEPNMGLPAANKAGYDAGSNVARASALRGRLKIMHGT
                     SDNMAPLSTTMRMAQALIEANRTFDLLIMPGQPHGPQGAAGRYYREDVRRFMATHLLG
                     ETYGAASQLH"
     gene            1978..3207
                     /locus_tag="VNU01_00930"
     CDS             1978..3207
                     /locus_tag="VNU01_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWH31212.1"
                     /translation="MISRRDFLGMSLGASATLALPPEVLRALQQSGGKLVQRAIPSSG
                     ERLPVVGLHFGNPPPPDHAALKAVLKTLLDSGGRFLDTVHQSAAAEEVTATIATELGV
                     QDTIFWSSRASPAGPPQPGAAAAKALMERSLVRFKVPKLDLVQMNPMADPGHLAALKE
                     MKQEGRVRYIGMQVIDDRQYAGLEAVMRKEPIDFIGIDYSIGNRGVEQTILPLAQERK
                     IGVVAYFPFDAGGLFQRVGTTPLPGWAADFDARTWAQFFLKYVISHPAVTVVRTGTSQ
                     AKHMLENIGGGTGRLPNQATRKRMAELVDSWPLAGTPGPSAPQGAAVVVPAAILDRYA
                     GEYKTASGSVTVRRSGSALHVKSGHTQEAPLVARSETRFSDPWGAIIEFQLDAAGTVT
                     GFIMQEGQLKLQGSRIR"
     gene            3276..>3547
                     /locus_tag="VNU01_00935"
     CDS             3276..>3547
                     /locus_tag="VNU01_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWH31213.1"
                     /translation="MVSQAGQTPVATGADWPQWRGTTRDGVIGAALPAQWPEALEKRW
                     EITVGTGHASPVVSGNRVVVIAREGDQEIVRALDAASGREIWRAAYA"
BASE COUNT          575 a         1180 c         1220 g          572 t
ORIGIN      
        1 gtgggcggcc cagggcccgc cgcgacactc ccgttgggac tcgcgtcttt tgcacctgag
       61 accgcggaac ccgccactcc ggcgcgatgg agggcgcagg tggcagacgg cgatctggtg
      121 gtgtctgaca gcggcggtgc cacacggctg aagttgccgg gagcgccgaa ctacgggtgg
      181 gccgtcgcgc cgcgggcctg gagcaatgac gggcagcact tcgtcgcgct gcgcgtcgac
      241 aaccgcgaga tccacagggt gcccatcgtc gactacacca gcgcgatcga gcgcgtcacg
      301 tgggcggcgt atccgaaaag cgggacgccg ttgcccgtca gcgaactgca catggtggat
      361 cctgcggcgg ccgtcgctcg gccggtgcaa ctggacacgc gggacgccta tgtctgggtg
      421 ctcggctggc ggccgacgaa gacggtgtcc gaagcactcg tgatgcggat gtcgcggacc
      481 ggcaagcgcc tggacttgtt tgcgatcgac actgccggcg cgacgcgtct gctcgcgcag
      541 gacgagcgtc cggcgtcgtt tgtggcggcg ctggactttt ccacgggcga ctgggcgtgg
      601 caggtcacgg cgcttcccga tgacagcggc ttcctctgga tgtccgagcg cgacggatgg
      661 cgccacgtct accactacgg ctacgacggc gccgtgaaga agcaggtgac gtcgggcgcc
      721 ttccccgttc accgcgttgt gcacgtcgat ccggctcggc gccagatcca cgtgctcgca
      781 tccggagagt ccgcgcgccc ctacgatcga cacgtgtacc gtgtcggcct ggacggcgcg
      841 gggttcaccg ccttgacgtc ggcgccagga ttacacgagc ccgcgttttc agcgtccggc
      901 gatttcttcg ccgacacgta ctccagcctg acgcagccgc acaccgtcga gctgcgccgc
      961 ggcgacggct cgctgatcaa ggttctgggg caggcctcaa cgataccgct cgaagacgtg
     1021 ggatacaggc cgccggagcc gttccgcgtg aaggcggctg atgggaccac cgatttatac
     1081 ggcgtcctgt accacccggc cgatttcgac cgcagcaagc gatacccgat cgtcgattac
     1141 atctacgccg ggccgttcat cgccgtgcat cagatgacat acatgccgtc ctcggccatg
     1201 caccgcatgt ccgcgtcgct cgcggagatg ggattcgtcg tcgtgatgct cgacgctcgc
     1261 ggcacgccgg gccgcggcaa ggcctttcag gacgcgaact acggtcgcat cggccagata
     1321 gaagttcccg accacgtcgc ggccatccgt gagctcgcca ggtccaagcc ctacatcgat
     1381 accacgcgtg ccgggatcgt cggccactcc tggggcggct acttcgcgct tcgcggaatg
     1441 ctgacggctc ctgatttctt caaagcgggg tatgcaggcg ccccgggtga tctgaccgaa
     1501 gcggccagca tcaacgagcc caacatgggt ctccccgcgg ccaacaaggc gggctacgac
     1561 gcgggatcca acgtggcgcg cgcgtccgcg ttgcgcggac ggctcaagat catgcacggg
     1621 acgagcgaca acatggcgcc tctctcgacc acgatgcgca tggcgcaggc gttgatcgag
     1681 gccaacagga cgttcgatct cctgatcatg ccgggccagc cacacggccc gcagggtgcg
     1741 gcaggacgct actacagaga ggacgtgcgc cgcttcatgg ccacccactt gctcggtgag
     1801 acatacggtg cagcgagcca gttgcactga agcgacggcc tgcagaccgc caacatacgg
     1861 gccgcgctgc cgtagacacc cggcgtgcgg gtcattgggc cgcactcgtg cgaggaacgg
     1921 ccaaggcgtc gcagccccgc cgtgcgcagt ggtcaaccac cacaggagag agggcggatg
     1981 atctcgcggc gtgattttct tggcatgtcc ctgggcgcca gcgctacgct ggcgcttccc
     2041 ccggaggtgc tccgcgcact tcagcagtcg ggcggcaagc tcgttcagcg ggcgattccg
     2101 tcttccggtg agaggctccc ggtcgtcggc ctccacttcg ggaacccccc gccgccagat
     2161 cacgccgcgc tcaaggcggt cctcaagacg ctgctcgaca gcggcggcag attcctcgac
     2221 acggtgcacc agtcggccgc ggcggaggag gtcaccgcca cgattgccac cgagctgggc
     2281 gtgcaggaca cgatcttctg gtcatcgcgc gcctccccgg ccggcccccc gcagccggga
     2341 gccgccgctg cgaaagcgct gatggagcgg tcgttggtga ggttcaaggt tcccaagctc
     2401 gacctcgtcc agatgaaccc catggccgat ccggggcacc tcgccgccct caaggagatg
     2461 aagcaggagg ggcgcgtccg gtacatcggc atgcaggtga tcgatgaccg ccagtacgcc
     2521 ggcctcgagg cggtgatgcg caaggagccg atcgatttca tcgggatcga ctactccatc
     2581 gggaatcggg gcgtggagca aacgatcctg ccgctcgcgc aggagcggaa gatcggcgtg
     2641 gtggcgtact tccccttcga cgccggcggc ctcttccagc gtgtcggcac cacgccgctt
     2701 ccgggttggg ccgccgattt tgatgccagg acgtgggcgc aattttttct caagtacgtc
     2761 atcagccacc cggccgtcac cgtggtgcgt acggggacca gccaggcgaa gcacatgctc
     2821 gagaacatcg gcggcggcac tgggcgcctg ccgaatcagg cgacgcggaa gcgcatggcg
     2881 gagctggtcg actcgtggcc gctggctggg acgcccgggc cgtcggcgcc gcaaggtgcc
     2941 gctgtcgtgg tgccggccgc catcctggac cgctatgccg gtgagtacaa gacggcatcc
     3001 gggtccgtca ccgtccgccg tagcggctcc gcgctgcatg tgaagtctgg ccacacgcag
     3061 gaggcgcccc tcgtcgcgcg ttcagagacc cgcttttccg atccctgggg agccatcatc
     3121 gaatttcagc tcgatgccgc cggcacggtg actggcttca tcatgcagga gggtcaactg
     3181 aagcttcagg gatcacggat ccgctgagga tgcacagacg cgccctggcg gtgttggccg
     3241 tactgatcgc actcgcccca gccggtgggg gcgggatggt ctcccaggcc gggcagacgc
     3301 cggtggccac tggcgctgac tggccacagt ggcgggggac gacgcgcgat ggtgtcatcg
     3361 gcgccgcctt gcccgcccaa tggccggaag cgctggagaa acgctgggag atcacggtcg
     3421 gcaccgggca tgcctcgccg gttgtctccg gcaaccgggt ggtggtcatc gcccgtgagg
     3481 gggatcagga gattgtccgc gccctcgatg cggcgtcggg cagggagatc tggcgggcag
     3541 cgtatgc
//
LOCUS       DATMKC010000032         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_114796, whole genome shotgun sequence.
ACCESSION   DATMKC010000032 DATMKC010000000
VERSION     DATMKC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114796"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1114)
                     /locus_tag="VNU01_00940"
     CDS             complement(143..1114)
                     /locus_tag="VNU01_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWH31214.1"
                     /translation="MSGAAALGPALVELRSALAAGAPPAVALQDALRRQPDALAALAR
                     PLRDMAVGMPLEQAAAALADDEGAAALLLRALAVAERAGVGASEAVAQGERAVAEELE
                     LARLLRVRTVRARGSLRVLALAPPAAVGFFLLLEPAAAGFYGTPLGRVSAAVSLALLL
                     VARRWARAIVDGVPAAVGRADPLGRAAGGGAAETAELLAVALAGGLPHAGAVRLVADL
                     GPPAARPVLRRAEAALAAGVAVDAAFAATPLRDLGAVLGAATRWGASARELLSAYAAG
                     LRADRRAALAEVAERTELRLVFPTTLLTLPSFLLAVVPPLLWTGLRR"
     gene            complement(1111..>2016)
                     /locus_tag="VNU01_00945"
     CDS             complement(1111..>2016)
                     /locus_tag="VNU01_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWH31215.1"
                     /translation="EGLLREPGVTDVLINGPREAWVERDGALVRVPLAFADGEELRQA
                     VQRVIGPLGLRLDRARPWVDARLPDGSRLHAVLPPLAPDGPLVTIRRFRTVPWRWEDL
                     VAEGALVAEAAALLRQAVRDRRAIVCCGPTGAGKTTLLGLLLSEVGAGERVVLIEEAA
                     ELRPACPHLVRMEALPPNVDGAGEVPLRELVRQALRMRPDRIVVGEVRGAEVLDVLQA
                     LATGHDGGMCTVHAGSAADALVRLEGMALLAGMPPAAVRAQLTVALDLLVVLRREPDG
                     RRRVAEIARVRDGARGPEVEAVWRP"
BASE COUNT          245 a          863 c          734 g          174 t
ORIGIN      
        1 tcagggtgcc gcaggcggcg gcgcccacac cgccgatcat ggcgtactcg gccgtctgcg
       61 agccgcgctc ctggcgcagc cgggacatgc ggtcgcggag caatcgggcc atgtgaccct
      121 cctttcgttc gtgcgccgcc gctcatcgcc gcagtcccgt ccacaggagg gggggaacga
      181 ccgcgagcag gaatgacggc agcgtcagca gcgtggtggg gaagacgagt cggagctcgg
      241 tgcgctcggc cacttcggcg agcgcggcgc ggcggtcggc gcgcaggccg gccgcatacg
      301 cggacagcag ctcccgcgcg ctggctcccc agcgcgtcgc ggcgcccagg accgcgccga
      361 ggtcccgcag cggggtggcg gcgaacgccg cgtcgaccgc caccccggcg gccagggcgg
      421 cctcggcgcg ccgcagcacc ggccgcgccg cggggggccc gaggtccgcg accaggcgca
      481 ccgcgccggc gtgcggaagg ccgccggcca gcgccaccgc gagcagttcg gccgtctccg
      541 cggcaccgcc cccggcggcg cggcccagcg ggtcggcacg gcccaccgcg gccgggacgc
      601 cgtccacgat cgcgcgcgcc cagcgccggg ccaccagcag cagcgccagc gacaccgcgg
      661 cgctcacacg gccgagcggc gtgccgtaga agccggccgc ggccggttcc agcagcagga
      721 agaagcccac cgccgctggt ggggccagtg ccagcacccg caggctcccc ctggcccgga
      781 cggtgcgcac gcgcagcagc cgggccagct ccagctcctc cgcgacggcg cgctcgccct
      841 gggccaccgc ctccgaggcg cccaccccgg cgcgttcggc caccgccagg gcgcgcagca
      901 gcagggccgc ggcgccctcg tcgtcggcga gcgccgccgc cgcctgctcc agcggcatgc
      961 ccaccgccat gtcgcgcagc gggcgcgcca gcgcggccag cgcgtccggc tgccgccgca
     1021 gcgcgtcctg caacgccacg gccggcgggg ctcccgccgc cagcgcgctg cgcagctcca
     1081 ccagcgcggg ccccagcgcc gccgcgccgc tcatgggcgc cacaccgcct ccacctcggg
     1141 accccgcgcg ccgtcccgca cgcgcgcgat ctcggccacg cggcggcggc cgtccggctc
     1201 ccggcgcagg accaccagca ggtccagcgc cacggtgagc tgcgcacgca ccgcggccgg
     1261 cggcatcccg gccagcagcg ccatgccctc cagccggacg agggcgtccg ccgccgagcc
     1321 ggcgtgcacc gtgcacatgc cgccgtcgtg tccggtggcc agcgcctgga gcacgtccag
     1381 cacctcggcg ccgcgcacct cccccacgac gatgcggtcg ggccgcatgc gcagggcctg
     1441 gcgcaccagc tcacgcagcg gcacttcccc cgcgccgtcg acgttcggcg gcagcgcctc
     1501 catgcgcacc aggtgcgggc aggccggccg cagctcggcg gcctcctcga tcagcaccac
     1561 gcgctcgccg gcgcccacct ccgacagcag caggccgagc agcgtggtct tgccggcgcc
     1621 cgtgggaccg cagcacacga tcgcgcggcg gtcgcggaca gcctggcgca gcagtgccgc
     1681 cgcctcggcg acgagggcac cctcggccac caggtcctcc cagcgccacg gcaccgtgcg
     1741 gaaccggcgg atggtcacca gcggcccgtc cggcgccagc ggcggcagca ccgcgtgcag
     1801 gcggctgccg tcgggcaggc gcgcgtccac ccagggccgc gcgcggtcca gccgcaggcc
     1861 gagcggcccg atgacccgct gcacggcctg ccgcagctcc tcgccgtcgg cgaaggccag
     1921 cggcacgcgc accagcgcgc cgtcgcgctc cacccacgcc tcgcgcggcc cgttgatgag
     1981 cacgtcggtg acgccgggct cccgcagcag tccctc
//
LOCUS       DATMKC010000033         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_308052, whole genome shotgun sequence.
ACCESSION   DATMKC010000033 DATMKC010000000
VERSION     DATMKC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308052"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="rfaE2"
                     /locus_tag="VNU01_00950"
     CDS             <1..850
                     /gene="rfaE2"
                     /locus_tag="VNU01_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HWH31216.1"
                     /translation="CGWVRLATPNRTELRAFAGPGGDGMAELTARADGLRARWRAAAL
                     AVTLGERGALLVDGGGMPLVVPAPHVAAGDPCGAGDRFASRAATALAGGALLSEAVTE
                     AVAAAAAFVAAGGASGFQAGAPDRRERPGSAVALAERVRAEGGTVVATGGCFDLLHAG
                     HVRMLESARALGDCLIVCLNSDASVRRLKGAGRPLTPQHDRAAVLLGLGCVDAVAIFE
                     EDTPETALRRLRPHVWAKGADYRADELPEARALTEWGGQAVVVPFVPGRSTTTIIEEA
                     TRRAHA"
     gene            837..1580
                     /locus_tag="VNU01_00955"
     CDS             837..1580
                     /locus_tag="VNU01_00955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH31217.1"
                     /translation="MPTPDATARPADPREPLGGLGRLRTVLVTGAASGLGRAVARAVA
                     EAGGTPVALDLSASPDGFDGEQVDLADTASAEAAVERLAERHGGLTAVVTAAGTDACG
                     KLADVERKSWEQVVAVNLLGTAAVVRAALPHLRASRGTVVTVASTLGLRALPDATAYS
                     ASKFGVVGFTRALALELAGEIGVTLLVPGGMSTAFFDGRPDQYKPGPDAQLNDPANVA
                     ASVLFALRQPPGCEVRELIVTPSTEPSWP"
     gene            1588..>2245
                     /locus_tag="VNU01_00960"
     CDS             1588..>2245
                     /locus_tag="VNU01_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31218.1"
                     /translation="MAGRPVLAALRALGLGDFLVAVPALRALARAHPGHRLVLLGPAS
                     LAPLVRLLDGWEPAPLEGKAGLDPGAPLPLAGPVDVAVNLHGRGPRSHALLRRLSPRR
                     LIAFAHPDVPGTQGLPPWVPDEVITSHERERWCGLLRSCGIDADAADLALPRPALPAP
                     VGGDDVTLIHVGAAGMSRRWPLERWAAVADTERRNGRRVYLTGSAAERPDALVVARWS
                     G"
BASE COUNT          214 a          861 c          901 g          269 t
ORIGIN      
        1 gtgcggttgg gtgcggctgg ccacacccaa ccgcaccgag ctgcgcgcct tcgccggtcc
       61 cgggggtgac ggcatggcgg agctgaccgc cagggccgac ggcctgcgcg cgcgctggcg
      121 cgccgcggcg ctggccgtga cgctcggcga gcgcggcgcg ctcctggtcg acggcggcgg
      181 catgccgctg gtggtcccgg caccgcacgt ggccgccggc gacccgtgcg gggcgggcga
      241 ccgcttcgcg tcccgcgccg ccacggcgct ggccggcggc gcgctgctgt ccgaggcggt
      301 gaccgaggcg gtcgccgccg cggcggcgtt cgtcgccgcc gggggggcct cgggattcca
      361 ggccggcgcg ccggaccggc gcgagcggcc cggctccgcg gtggcgctgg ccgagcgcgt
      421 gcgcgccgag ggcggcacgg tggtggccac cggcggctgc ttcgacctgc tgcacgccgg
      481 ccacgtgcgg atgctggagt cggcccgggc actgggcgac tgcctgatcg tgtgcctgaa
      541 ctccgacgcg tcggtgcgcc gcctcaaggg cgcgggccgc ccgctcacgc cgcagcacga
      601 ccgcgcggcg gtgctgctgg ggctgggctg cgtcgacgcg gtggcgatct tcgaggagga
      661 cacgccggaa acggcgctgc ggcgcctgcg cccccacgtg tgggccaagg gtgccgacta
      721 ccgcgccgac gagctgccgg aggcgcgcgc gctgacggag tggggcgggc aggccgtcgt
      781 ggtcccgttc gtgccgggcc gctccaccac cacgatcatc gaggaggcca cccgccgtgc
      841 ccacgcctga cgccaccgcc cgtcccgccg acccacgcga gccgctcggc ggcttgggcc
      901 gcctgcgcac cgtgctggtg accggcgcgg cctccgggct gggccgcgcg gtggcgcgcg
      961 cggtcgccga ggcgggcggc acaccggtcg cgctggacct gtcggccagc ccggacggct
     1021 tcgacggcga gcaggtcgac ctggccgaca ccgcgtcggc ggaggccgcg gtcgagcgcc
     1081 tggccgagcg ccacggcggg ctcaccgccg tggtcaccgc cgcgggcacg gacgcgtgcg
     1141 gcaagctcgc cgacgtcgag cgcaagtcct gggagcaggt cgtggccgtg aacctgctgg
     1201 gcacggcggc cgtggtgcgc gcggcgctgc cccacctgag agccagccgc ggcaccgtgg
     1261 tcacggtggc ctcgacgctc ggcctgcgcg ccctgcccga cgccaccgcc tactcggcca
     1321 gcaagttcgg cgtggtcggc ttcacccgcg cgctggccct ggagctggcg ggcgagatcg
     1381 gcgtgaccct gctggtgccc ggcggcatgt ccacggcgtt cttcgacggc cgtcccgacc
     1441 agtacaagcc cggcccggac gcacagctca acgacccggc gaacgtggcg gcgtccgtgc
     1501 tgttcgcgct gcgccagccg cccggctgcg aggtgcgcga gctgatcgtg acgccgtcga
     1561 ccgagccgag ctggccgtag gtccgccatg gccggccgtc ccgtcctcgc cgccctgcgg
     1621 gccctcgggc tcggtgactt cctggtcgcc gtgccggcgc tgcgcgcgct ggcgcgcgcc
     1681 catcccggtc accgcctggt cctgctgggg ccggcgtccc tggcgccgct ggtgcggctg
     1741 ctcgacggct gggagccggc gccactggag ggcaaggccg gcctggatcc cggcgcgccg
     1801 ctgccgctgg ccgggcctgt ggacgtcgcg gtcaacctgc acgggcgcgg gccgcggagc
     1861 cacgcgctgc tgcggcggct gtcgccgcgg cggctgatcg cgttcgcgca ccccgacgtg
     1921 cccggcaccc agggcctgcc gccgtgggtg ccggacgagg tgatcacgtc acacgagcgc
     1981 gagcgctggt gcgggctgct gcgctcctgc ggcatcgacg ccgacgcggc ggacctggcg
     2041 ctgccgcgcc cggcgctgcc ggcgccggtg ggaggcgacg acgtgacgct catccatgtc
     2101 ggggcggccg gcatgagccg acgctggccg ctggagcgct gggcggcggt cgccgacacc
     2161 gaacgccgca acggccgccg cgtgtatctc acgggcagcg cggcggagcg ccccgacgcg
     2221 ctcgtggtcg cgcgctggtc cggcc
//
LOCUS       DATMKC010000034         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_307949, whole genome shotgun sequence.
ACCESSION   DATMKC010000034 DATMKC010000000
VERSION     DATMKC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307949"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /locus_tag="VNU01_00965"
     CDS             complement(<1..2019)
                     /locus_tag="VNU01_00965"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HWH31219.1"
                     /translation="MNKPVDLSVGRLAEHDDEHCNPSANPRFSEVLDARLSRRSMLRG
                     GVGTAAATVLGGLGLAACGGGGDAGAGASESAQRAIGARPKALEPIRSLRFTPVAKSI
                     ADAVSVPVEYDYGIVIALGDPLDAVTPDYRNDGSDSDFERRSGDHHDGMEYFGLSADG
                     KKRDVNGSNRGLIAMNHEATSHPRGWRPSIFIHPTGGDNGAVRPAAEVEKEFAIHGLS
                     VQEVRKQGATFAYVRGSRYNRRVTAMTPVEIAGPLRGHPLMVTRYDPAGLTTRGTLNN
                     CGTGCTPWGTYLSGEENWAGYYTRSATDDAARGHDKSTVALRRYGRAQGAASRHGWET
                     PVDPTNADRYARWNISRTGASADGSDDYRNELNGQGWIVELDPYDPNAAVRKRTALGR
                     FAHESAVFGPRQPGQPLAVYMGDDSQGEYMYKFVSAAKWTPADAEPKNRLAAGDKFLD
                     HGRLYVAKYHADGTGEWVELSISHPAIASYAGYAFADQADVLVNARLAADAVGATKMD
                     RPEWCGVNPANGEVYVALTNNSNRKLEPSGTQFGLDAANPRSYVDQDGPGTSAGNVNG
                     HIIRMAEAGGASAATRFTWDVYLFGAEADALASVNLSGLSDSQDFSSPDGLWFSPSTG
                     VCWIQTDDGAYTNETNCMMLAGLPGRVGDGAVRTLNYTKADGSVVTIDT"
BASE COUNT          257 a          726 c          788 g          344 t
ORIGIN      
        1 ggtgtcgatc gtgacgacgc tgccgtcggc cttggtgtag ttcagcgtgc gcaccgcgcc
       61 gtcgccgacg cggcccggca ggccggcgag catcatgcag ttggtctcgt tggtgtacgc
      121 gccgtcatcg gtctggatcc agcacacgcc ggtgctcggg ctgaaccaca ggccgtcggg
      181 cgacgagaag tcctggctgt cgctcaggcc cgagaggttc acgctggcca gcgcatcggc
      241 ctcggcgccg aacaggtaga cgtcccaggt gaagcgcgtc gccgccgacg cgccgccggc
      301 ctcggccatg cggatgatgt ggccgttgac gttgccggcc gaggtccccg ggccatcctg
      361 gtccacgtag gagcgcgggt tggccgcgtc cagcccgaac tgcgtgccgc tgggctcgag
      421 cttgcggttg ctgttgttgg tcagcgcgac atagacctcg ccgttggccg ggttgacgcc
      481 gcaccactcg gggcggtcca tcttcgtggc gccgaccgca tcggcggcga gtcgcgcgtt
      541 cacgagcacg tcggcctggt cggcgaaggc atagccggcg tagctggcga tcgccgggtg
      601 gctgatcgac agctcgaccc actcgcccgt gccatcggcg tggtacttgg ccacgtagag
      661 gcggccatgg tccaggaact tgtcgccggc ggccaggcgg ttcttcggct cggcgtcggc
      721 cggcgtccac ttggcggcgc tgacgaactt gtacatgtac tcgccctgcg agtcgtcgcc
      781 catgtagacc gccagcggct ggcccggctg gcgcggaccg aacaccgcgc tctcgtgcgc
      841 gaagcggccc agcgccgtgc gcttgcgcac cgcggcgttc gggtcgtagg gatccagctc
      901 gacgatccag ccctggccat tgagctcgtt gcggtagtcg tcgctgccgt cggccgaggc
      961 gccggtgcgg ctgatgttcc agcgcgcgta gcggtcggcg ttggtcgggt cgaccggggt
     1021 ctcccagccg tggcggctgg ccgcgccctg ggcgcgaccg tagcggcgca gcgcgacggt
     1081 gctcttgtcg tggccacgcg cggcatcgtc ggtggcgctg cgggtgtagt agccggccca
     1141 gttctcctcg ccgctcaggt aggtgcccca gggcgtgcag ccggtgccgc agttgttcag
     1201 cgtgccacgg gtcgtcaggc ccgccgggtc gtaccgggtc accatcagcg ggtggccgcg
     1261 cagcggaccg gcgatctcca ccggcgtcat cgcggtgacg cggcggttgt agcgcgagcc
     1321 gcgcacgtag gcgaaggtcg cgccctgctt gcggacctcc tgcaccgaca ggccgtggat
     1381 ggcgaactcc ttctcgacct cggccgccgg gcgcaccgcg ccgttgtcgc cgccggtcgg
     1441 gtggatgaag atcgacggac gccagccgcg cgggtggctg gtggcctcgt ggttcatcgc
     1501 gatcaggccg cggttcgagc cgttgacgtc gcgcttcttg ccgtcggcgc tcaggccgaa
     1561 gtactccatg ccgtcgtggt ggtcgcccga gcggcgctcg aagtcgctgt cgctgccgtc
     1621 gttgcggtag tcgggcgtga cggcgtcgag cggatcgccg agcgcgatga cgatgccgta
     1681 gtcgtactcc accggcacgc tgaccgcgtc ggcgatgctc ttggccaccg gcgtgaaacg
     1741 cagcgagcgg atcggctcga gggccttcgg gcgggcgccg atcgcgcgct gcgccgactc
     1801 gctcgcgccg gctcccgcgt cgcccccgcc gccgcaggcc gccaggccca ggccgccgag
     1861 caccgtggcc gccgccgtgc cgacgccgcc gcgcagcatc gaacgccggc tcaggcgcgc
     1921 gtccagcacc tcgctgaagc ggggattggc actggggttg cagtgctcgt cgtcatgctc
     1981 ggcgaggcgg ccgacggaaa ggtcaacggg cttgttcatg gggttccttg caaaggcggg
     2041 gcccgcggcg gggcgacggc aaaccttcgc cacggtaggc agcccgcatg aaagcgccgt
     2101 gacatcgtgg tgaaa
//
LOCUS       DATMKC010000035         2996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_44007, whole genome shotgun sequence.
ACCESSION   DATMKC010000035 DATMKC010000000
VERSION     DATMKC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44007"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VNU01_00970"
     CDS             <1..547
                     /locus_tag="VNU01_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31220.1"
                     /translation="VEGMPALSANQEGIARLLDALRGFSTELGTAFRIARPDIDRMIV
                     GGDNVLRVLYHYRLEVGQVVDGLVLYTEKFGPGFTSPGVTGQAAYFQIILEGEDFQGE
                     FCSGMPEQMREQFPFCTGEASGEVRAGLPRDLARPEVPQARPLTELLRRALGGMGVPR
                     ESQQGSQQGSQQGSGQGGEGA"
     gene            544..1662
                     /locus_tag="VNU01_00975"
     CDS             544..1662
                     /locus_tag="VNU01_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWH31221.1"
                     /translation="MSDRHPAIKFGVFALVCTLCSVWLISVTGNIRFFARTSSYEAVM
                     DDATGLLKRDSVLLAGVRVGTVEDVSVERGKAVVRFKVDRGIELRDTWEVGIRWRNVI
                     GQRFLYLYPVGGGEPLQAGDRLPAEQSRPTADIGRFFERLTPLLEAIDPEQQNKLLAA
                     LNEALEGKQERIQELVADLGSLSSTLADREAKIRTVIGQGAALLDAYADRDQQIRQFL
                     TDFADVSRTLRARNDVLVGAVADAGDVQQRLDELLRANDTDIRTTLDGLDDIASRIGT
                     HRQDVENALATLKDGFATYMLISRWGQWFNVRAVATQVQNDGQVQSCQAEEGHPCEVP
                     NSRPSGGANASWRVPGRNAALPRLVSTALAPGMGGGGR"
     gene            1659..2846
                     /locus_tag="VNU01_00980"
     CDS             1659..2846
                     /locus_tag="VNU01_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWH31222.1"
                     /translation="MNLTFLERNQVVIGAVAAAAILAAVIGGLVVQGGIFQGGYTLQA
                     QFANAAGLSAGDHVFVAGVRAGTVDGLELAGDHVLVTFNVDTELPDQTRAKIILRTLV
                     GARAMELVPSGDWERPLGDGDVIPLDRTDVPVDIPEFGEVAEDLLSQTDTAALDEFLK
                     AVTEITRGQRVQVGALIEGGRRLATVVSDQEQQVRRLIRGLRSLGETLQARDAELAVI
                     VDDFGDVVAELAARREELRRFLRATNSASAAAADLVRDERDELDRILSNVHIASDIVA
                     RHQLDLAEALAYSGDAITGFASISYSGAQKVPWGYVFTTSLGTVGVDTITGCGGLFDQ
                     YLDELLGPDPRSCAEQDNSTFPEDTPNADEDASASLPLSAVARRALPVRDALRLLAGE
                     VAP"
BASE COUNT          414 a         1079 c         1100 g          403 t
ORIGIN      
        1 ggtcgagggc atgccggcgc tgtcggcgaa ccaggagggc atcgcgcggc tgctcgacgc
       61 gctgcgcggc ttctccaccg agctgggcac cgcgttccgc atcgcgcggc ccgacatcga
      121 ccgcatgatc gtgggcggcg acaacgtcct gcgcgtgctg taccactacc gcctggaggt
      181 cgggcaggtg gtggacggcc tggtgctgta caccgagaag ttcggccccg gcttcaccag
      241 cccgggggtc accggccagg cggcgtactt ccagatcatc ctggagggcg aggacttcca
      301 gggcgagttc tgctccggca tgcccgagca gatgcgcgag cagttcccgt tctgcaccgg
      361 cgaggcgagc ggcgaggtgc gcgccgggct gccgcgcgac ctggcccggc cggaggtgcc
      421 gcaggcgcgc ccgctgacgg agctgctgcg ccgcgcgctg ggcggcatgg gcgtgccccg
      481 ggagagccag cagggaagcc agcagggaag ccagcaggga agcgggcagg ggggtgaggg
      541 cgcgtgagcg accgtcaccc cgccatcaag ttcggcgtct tcgcgctggt gtgcaccctg
      601 tgctcggtgt ggctgatctc ggtcaccggc aacatccgct tcttcgcgcg cacgtccagc
      661 tacgaggcgg tgatggacga cgccaccggc ctgctcaagc gcgacagcgt gctgctggcc
      721 ggcgtgcgcg tcggcaccgt cgaggacgtc tccgtggagc gcggcaaggc ggtggtgcgc
      781 ttcaaggtgg accgcggcat cgagcttcgc gacacctggg aggtggggat ccgctggcgc
      841 aacgtgatcg ggcagcgctt cctgtacctg tacccggtgg gcggcggcga gccgctccag
      901 gcgggtgatc gactgccggc cgagcagagc cggcccaccg cggacatcgg ccgcttcttc
      961 gagcggctca ccccgctgct tgaggccatc gaccccgagc agcagaacaa gctgctggcg
     1021 gcgctgaacg aggcgctgga aggaaagcag gagcgcatcc aggagctggt ggccgacctg
     1081 ggctcgctga gctccacgct ggccgaccgc gaggccaaga tccgcacggt catcggccag
     1141 ggcgccgcac tgctggacgc ctacgccgac cgcgaccagc agatccgcca gttcctcacc
     1201 gacttcgccg acgtgtcgcg cacgctgcgg gcgcgcaacg acgtgctggt cggcgccgtg
     1261 gccgacgccg gcgacgtgca gcagcgcctg gacgagctgc tgcgcgccaa cgacaccgac
     1321 atccgcacga cgctggacgg cctggacgac atcgcctcgc gcatcggcac gcatcgccag
     1381 gatgtggaga acgccctggc cacgctcaag gacggcttcg ccacctacat gctgatcagc
     1441 cgctggggcc agtggttcaa cgtccgcgcg gtcgccacac aggtccagaa cgacggccag
     1501 gtgcagtcct gccaggccga ggagggccac ccctgcgagg tgcccaactc gcgcccgagc
     1561 ggcggcgcca acgcctcctg gcgcgtgccg ggccgcaacg ccgccctgcc ccgcctggtc
     1621 agcaccgccc tggcccccgg gatggggggt ggtgggcggt gaacctcacc ttcctggagc
     1681 gcaaccaggt ggtcatcggc gccgtcgcgg cggccgcgat cctggccgcc gtcatcggcg
     1741 ggctggtggt ccagggcggc atcttccagg gcggttacac gctccaggcg cagttcgcca
     1801 acgcggccgg gctgtcggcc ggcgaccacg tcttcgtcgc gggcgtgcgc gcgggcaccg
     1861 tggacggcct ggagcttgcc ggcgaccacg tcctggtgac gttcaacgtg gacacggagc
     1921 tgccggacca gacccgggcc aagatcatcc tgcgcacgct ggtgggcgcc cgcgcgatgg
     1981 agctggtgcc cagcggggac tgggagcggc cgctgggcga cggcgacgtc atccccctgg
     2041 accgcaccga cgtgccggtc gacatccccg agttcggcga ggtcgccgag gacctgctga
     2101 gccagaccga caccgccgcg ctcgacgagt tcctcaaggc ggtgaccgag atcacccgcg
     2161 ggcagcgcgt ccaggtcggc gcgctgatcg agggcggccg ccgcctggcc accgtggtca
     2221 gcgaccagga gcaacaggtg cggcggctga tccgcgggct gcgctccctg ggcgagacgc
     2281 tccaggcgcg cgacgcggag ctggccgtca tcgtggacga cttcggcgac gtggtggccg
     2341 agctcgccgc ccgccgggag gagctgcgcc gcttcctgcg cgcgaccaac agcgcgagcg
     2401 cggccgcggc ggacctggtg cgcgacgagc gcgacgagct ggaccggatc ctgtccaacg
     2461 tgcacatcgc gtccgacatc gtcgcccgcc accagctcga cctcgccgag gccctggcgt
     2521 actcaggcga cgcgatcacc ggcttcgcgt cgatctccta ctcgggcgcg cagaaggtgc
     2581 cgtggggcta cgtgttcacc acgtcgctcg gcacggtcgg tgtggacacc atcaccggct
     2641 gcggcggcct gttcgaccag tacctggacg agctcttggg accggacccg cgctcctgcg
     2701 ccgagcagga caactccacg ttcccggagg acacgcccaa cgccgacgag gacgcctcgg
     2761 cctcgctgcc cctgtccgcg gtcgcccgcc gggcgctgcc ggtccgtgac gcgctgcggc
     2821 tgctggcggg ggaggtggcg ccgtgaccct gtccgcccgc ggccgcctgc tcgccgtgcc
     2881 gctgctgctc gtgctcgccc tgctggccag cgcctgctcg ctgaaccgcg ccggcggggg
     2941 gcgtgcgctg gacgccatcg tcgcgcgctc caacaacctg ttcgtgggct cggacg
//
LOCUS       DATMKC010000036         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_307801, whole genome shotgun sequence.
ACCESSION   DATMKC010000036 DATMKC010000000
VERSION     DATMKC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307801"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1849)
                     /locus_tag="VNU01_00985"
     CDS             complement(<1..1849)
                     /locus_tag="VNU01_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWH31223.1"
                     /translation="MQLRTMAFERDAGGIVTLTLDDRERRVNTMRTEFLDDFESVLDA
                     LRRDAEGLRGVIVTSAKSTFFAGGNVDELFAAGRDDAAAIAESSRRAKAVLRQLETLD
                     VPVAAALNGSALGGGLEIALACHFRVALDNSTLRLGFPEVTLGLLPGAGGVVRSVRLL
                     GLAAALDLMLSGRQLNARAALAAGLVQDLAANTAAMLDRCRRWIAEGRVEDARLDEAG
                     YADPWLAPFGPASMAETMMLPSRLVKSINGRPDTARRSILCAALESVQVDLDSAFLIE
                     TRYFTELVCASQQAKNLIQSGFYDTRAIKAGLNRPADVPQWRATKVGVVGAGMMGAGI
                     AYVCAAAGLPVVLKDVSAEKAAQGRDYSARFAAKQVQRGELSAEQAAGLVERIRATAS
                     YDDLRGCDVVIEAVFESAELKRKVFAEVEAVVGPEALLSSNTSSLPVTELAGGVSRPA
                     DFLGLHFFSPVPRMALLEIVRGAETSDEALARAIDFSMQIAKTPIIVNDSRGFFTSRV
                     IATFLYEAVAAVGEGVPAALVERAGLMAGYPAAPLSLLDDLTLTLPLQIADEARAARE
                     AAGRPYEEHPGLGVLRRMVALPRHGRAAGAGFYDYAEDGSRLGLWSGLAQ"
     gene            complement(1877..2194)
                     /locus_tag="VNU01_00990"
     CDS             complement(1877..2194)
                     /locus_tag="VNU01_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31224.1"
                     /translation="MLTGPAPACRLALARAGMSIADVDLVEINEAFASIALKLMDDLG
                     VDPSITNVNGGAIAMGHPLGATGAMLVGTILDELEARDLTVGLVTLCVGGGMGIATVV
                     ERV"
BASE COUNT          336 a          820 c          784 g          307 t
ORIGIN      
        1 gctgcgccag cccgctccac aggcccagcc gtgagccgtc ctcggcgtag tcgtagaagc
       61 cggcgcccgc agcgcggccg tgacgcggca gggcgaccat gcgccgcagc acgccgaggc
      121 ccggatgctc ctcatagggc cgcccggcgg cctctcgggc cgcccgcgcc tcgtcggcga
      181 tctgcagcgg cagcgtcagc gtgaggtcgt cgagcagact cagcggcgct gcggggtaac
      241 cggccatcag cccggcccgc tccaccagcg cggcgggcac gccttcacct acggcggcga
      301 ccgcctcgta caggaaggtg gcgatcacgc ggctggtgaa gaaaccgcgc gagtcgttga
      361 cgatgatggg cgtcttggcg atctgcatcg agaagtcgat ggcgcgcgcg agcgcctcgt
      421 cggaggtctc ggcgccccgc acgatctcca gcagcgccat gcgcggcacc ggcgagaaga
      481 agtgcaggcc caggaagtcc gccgggcgcg acacgccgcc agccagctcg gtgaccggca
      541 gggaggaggt gttggacgac agcagcgcct caggtccgac caccgcctcg acctccgcga
      601 agaccttccg cttcagttcg gcggactcga agaccgcttc gatcaccacg tcgcagccgc
      661 gcaggtcgtc atacgacgcg gtggcgcgga tccgctccac caggcccgcg gcctgctcgg
      721 cggacagctc gccgcgctgc acctgcttgg cggcgaagcg cgcggagtag tcgcgcccct
      781 gcgccgcttt ttccgccgac acgtccttga gcaccaccgg cagcccggcg gcggcgcaga
      841 cgtaggcgat tccggccccc atcatcccgg cccccaccac gccgaccttc gtggcccgcc
      901 actgcggcac gtccgccggg cggttgagcc cggccttgat ggcgcgggtg tcgtagaagc
      961 cggactggat gaggttcttg gcctgctggg aggcgcacac cagctcggtg aagtagcgcg
     1021 tctcgatgag gaaggcgctg tcaagatcca cctggacgct ctccagcgcc gcgcagagga
     1081 tgctgcgtcg cgcggtgtcc gggcggccat tgatgctctt gaccagccgc gacggcagca
     1141 tcatggtctc ggccatggaa gcggggccga acggggcgag ccaggggtct gcgtagccgg
     1201 cctcgtccag gcgcgcgtcc tccacgcggc cctcggcgat ccagcggcgg cagcggtcca
     1261 gcatcgccgc ggtgttcgcg gccaggtcct gcacgaggcc cgccgccagc gccgcgcggg
     1321 cgttgagctg gcgcccggac agcatgaggt cgagcgcagc ggccagtccc agcaaccgca
     1381 cggaacgcac gacgccgccg gcgcccggga gcaggcccag cgtcacctcc ggaaatccca
     1441 gtcgcaacgt ggagttgtcg agggcgacgc ggaagtggca ggccagcgcg atctccagcc
     1501 cgccccccag ggcgctgccg ttcagcgcgg cggcgacggg cacgtcgaga gtctcaagct
     1561 ggcgcaagac cgctttggcc cggcgcgagc tctccgcgat cgccgccgcg tcgtcccgac
     1621 cagcggcgaa cagttcgtcc acgttgcccc cggcgaagaa cgtgctcttg gccgacgtga
     1681 cgatgacacc acgcaacccc tccgcgtcgc gtcgcagcgc atccagcacg ctctcgaagt
     1741 cgtcgaggaa ctcggtgcgc atcgtgttga cccggcgctc gcggtcgtcc agggtcagcg
     1801 tgacgatgcc gccggcgtcg cgctcgaacg ccatggtccg cagttgcatg ctccagccct
     1861 tttctcggtc ggggtcctag actcgttcga cgaccgtggc gatgcccatg ccgccaccca
     1921 cacagagggt caccagcccg acggtgaggt cccgcgcttc cagctcgtcc agaatcgtgc
     1981 cgaccagcat ggcgccggtc gctcccaggg ggtgacccat cgcgatggcg cccccgttga
     2041 cgttggtgat ggaagggtcg acgccgaggt cgtccatcag cttgagcgcg atggaggcga
     2101 acgcctcatt gatctcgacc aggtccacgt ccgcgatgct catcccggcg cgcgcgagcg
     2161 ccagccggca cgcgggcgcc ggaccggtca gcatgatcgg cggccgtggt gggctcggag
     2221 ccgacgatca tgctgaccgg tccggcg
//
LOCUS       DATMKC010000037         2970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_43822, whole genome shotgun sequence.
ACCESSION   DATMKC010000037 DATMKC010000000
VERSION     DATMKC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43822"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1266)
                     /locus_tag="VNU01_00995"
     CDS             complement(64..1266)
                     /locus_tag="VNU01_00995"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWH31225.1"
                     /translation="MSKRLVLAYSGGLDTSVAIRWLEEHKGYEVIACAIDVGQAAPGE
                     MDRVRQRGLDCGAVESVVVDAKQQFADDFVAPAIAANALYMGKYPLVSSLSRPLIVSH
                     LIRVARETGAEAIGHGCTGKGNDQVRFEVGAMALAPDVKVEAPIREWGLSREAAIAWA
                     NERGIPIPVKKGSPYSIDENLWGRTAECGILEDPWESPPEEVYERTVSAAAAPDEAEE
                     LVIAFERGLPVAVDGKELDLATLIAEVDRRAGAHGVGRIDMVEDRLVGIKSREIYECP
                     GAITLLTAHRDLEDLCLEQELAQEKRGLEGRYAQLVYNGLWFSPLKKALDAFIGESQR
                     YVTGEVRMRLHKGSASVAGRRSPTGLYDLDLATYDEGDQFDQTQAEGFVKLWGLPTKV
                     WSRRQGSL"
     gene            complement(1259..1771)
                     /locus_tag="VNU01_01000"
     CDS             complement(1259..1771)
                     /locus_tag="VNU01_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HWH31226.1"
                     /translation="MTVPTLNGKRHRQELLRQLVAQYAVGSQGELVALLARHGVDATQ
                     ATVSRDLEELAIDKIRGADGRESYVLPEPMGLQRILRQFVLGVDASANLAVVRTPPGA
                     AGTVASAVDQAGVPGVLATVQGDDTILVVAREGLTGREVADRLLALKESRPPVPQDPR
                     QDSQMEKHHV"
     gene            complement(1768..2694)
                     /gene="argF"
                     /locus_tag="VNU01_01005"
     CDS             complement(1768..2694)
                     /gene="argF"
                     /locus_tag="VNU01_01005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWH31227.1"
                     /translation="MTTRHFLSLDGLSSEDLSKLLDSADELKAQRAGGRVQRDDLAGL
                     SIGMLFEKPSTRTRISFEVAIAELGARPVLLDARQMQLGRGEPLEDTARILSGYLHAL
                     VIRTFEQSRLETLATVGSIPVVNALSDFVHPCQALADLQTIREYKGTLPGLRVAYLGD
                     GNNVAHALLTAGALAGMHVSVGTPAGYEPIEQVVRSAREIAAGTGGSVTITTDPVEAA
                     RGADVVYTDVWASMGQESEHAARVLMFQPFQVNGRILEQARPDAIVMHCLPAHRGEEI
                     TADVIDGQQSVVWAQAENRLHSQKALLLDLLG"
     gene            complement(2691..>2970)
                     /locus_tag="VNU01_01010"
     CDS             complement(2691..>2970)
                     /locus_tag="VNU01_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH31228.1"
                     /translation="DGLEGLLKIRMRRPAVVVLDLAMPAVGGLRLLDELEEEGSGVPV
                     VVVTGDMAAAAEARRRLGDANVLLKPFDVDDLTRRLRHLARGEGVTKP"
BASE COUNT          375 a         1051 c         1110 g          434 t
ORIGIN      
        1 cgggcggcgg gtcccctgcg agagcgccga ggagttccgg cccgcggcga cacgccgacc
       61 ggtctagagc gagccctgcc tccgggacca caccttggtg ggcaggcccc agagcttcac
      121 gaagccctcc gcctgggtct ggtcgaactg gtcgccttcg tcgtaggtgg ccaggtccag
      181 gtcgtacagg ccggtgggcg agcggcggcc ggcgacggac gcgctcccct tgtgcaggcg
      241 catgcgcacc tcgccggtga cgtagcgctg ggactcgccg atgaacgcgt ccagcgcctt
      301 cttcagcggc gagaaccaca ggccgttgta gacgagctgg gcgtagcggc cctccagccc
      361 gcgcttctcc tgcgcgagct cctgctccag gcacaggtcc tcgaggtcgc ggtgggcggt
      421 gagcagcgtg atggcgccgg ggcactcgta gatctcgcgg ctcttgatgc ccacgagccg
      481 gtcctcgacc atgtcgatgc ggccgacgcc gtgcgcgccg gcgcggcggt ccacctcggc
      541 gatgagcgtg gcgaggtcca gctccttgcc gtccacggcc acgggcaggc cgcgctcgaa
      601 ggcgatgacg agctcctcgg cctcgtcggg ggcggcggcg gcggagacgg tgcgctcgta
      661 gacctcctcc ggcggggact cccacgggtc ctccaggatc ccgcactcgg cggtgcggcc
      721 ccacaggttc tcgtcgatgg agtacggcga gcccttcttc accgggatcg ggatgccgcg
      781 ctcgttcgcc catgcgatgg cggcctcgcg cgacaggccc cactcgcgga tgggcgcctc
      841 caccttgacg tcgggggcca gcgccatggc gccgacctcg aagcgcacct ggtcgttgcc
      901 cttgccggtg cagccgtggc cgatcgcctc ggcgcccgtc tcgcgggcca cccggatgag
      961 gtgggacacg atcagcggcc gcgacagcga cgacaccagc gggtacttgc ccatgtacag
     1021 cgcgttggcg gcgatggccg gggcgacgaa gtcgtcggcg aactgctgct tggcgtcgac
     1081 gacgacggac tccacggcgc cgcagtccag gccgcgctgg cgcacgcggt ccatctcgcc
     1141 gggtgccgcc tggccgacgt cgatcgcgca ggcgatcacc tcgtagccct tgtgctcctc
     1201 cagccagcgg atcgccacgg aggtgtcgag gccgccggag taggccagga cgagacgctt
     1261 agacatggtg cttctccatc tgagagtcct gccgggggtc ctgcgggacg ggtggacggg
     1321 attccttgag ggccaggagg cggtcggcga cctcgcggcc ggtcagccct tcgcgggcga
     1381 ccaccaggat ggtgtcgtcg ccctgcacgg tcgcgagcac gccgggcacg cccgcctggt
     1441 cgacggcgct ggcgacggtg ccggccgcgc cgggcggcgt gcgcaccacg gcgaggttcg
     1501 cgctggcgtc cacgccgagc acgaactgcc gcaggatgcg ctgaagcccc atcggctcgg
     1561 gcagcacgta gctctcgcgg ccgtcggcgc cgcggatctt gtcgatcgcg agctcctcca
     1621 ggtcgcggct caccgtggcc tgcgtcgcgt cgacgccgtg gcgggcgagc agggcgacga
     1681 gctcgccctg ggacccgacg gcgtactgcg ccacgagctg gcgcaggagc tcctggcggt
     1741 ggcgcttgcc gttcagcgtc ggcacggtca tcccagcagg tccagcagca gcgccttctg
     1801 ggagtgcagg cggttctccg cctgggccca gacgacgctc tgctggccgt cgatgacgtc
     1861 cgcggtgatc tcctcgccgc ggtgcgcggg caggcagtgc atgacgatcg cgtcggggcg
     1921 cgcctgttcc aggatccgcc cgttgacctg gaacggctgg aacatcagca cgcgcgcggc
     1981 gtgctcggac tcctggccca tgctggccca gacgtcggtg tagaccacgt cggcgccgcg
     2041 cgcggcctcg acggggtcgg tggtgatcgt gacgctgccg ccggtgccgg ccgcgatctc
     2101 ccgggcgctg cgcaccacct gctcgatcgg ctcgtagccg gcgggggtgc cgacgctgac
     2161 gtgcatgccc gccagcgccc cggcggtgag cagggcgtgc gcgacgttgt tgccgtcgcc
     2221 caggtacgcc acgcgcagtc ccggcagcgt gcccttgtac tcgcggatgg tctgcaggtc
     2281 ggccagcgcc tggcacgggt gcacgaagtc ggacagggcg ttgacgacgg ggatgctgcc
     2341 gacggtcgcc agggtctcca ggcgcgactg ctcgaacgtg cggatgacca gggcgtgcag
     2401 gtagccggac aggatgcgcg cggtgtcctc gagcggctcg ccgcggccga gctgcatctg
     2461 ccgggcgtcc agcagcacgg ggcgggcgcc cagttcggcg atggcaacct cgaaggagat
     2521 gcgcgtgcgc gtggacggct tctcgaacag catgccgatc gacaggccgg ccaggtcgtc
     2581 gcgctgcacg cgcccgccgg cgcgctgggc cttgagctcg tcggcgctgt cgagcagctt
     2641 gctgaggtcc tcgctgctca gcccgtcgag gctgaggaag tggcgcgtgg tcatggcttg
     2701 gtgactcctt cgccgcgcgc caggtggcgc agccggcgcg tgaggtcgtc gacgtcgaag
     2761 ggcttgagca gcacgttggc gtctcccagg cgccggcgcg cctcggcggc cgcggccatg
     2821 tcgccggtga cgacgacgac gggcacgccg gagccctcct cctccagctc gtccagcagg
     2881 cgcagcccgc cgaccgccgg catggccagg tcgagcacga ccacggcggg gcggcgcatg
     2941 cggatcttca gcaggccctc caggccgtcc
//
LOCUS       DATMKC010000038        10105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_70339, whole genome shotgun sequence.
ACCESSION   DATMKC010000038 DATMKC010000000
VERSION     DATMKC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70339"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VNU01_01015"
     CDS             <1..1561
                     /locus_tag="VNU01_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWH31229.1"
                     /translation="AAEGDPGAVVDPGEELIGAPEPEPAPESAVRLDATLFMAGVPDS
                     SRQQVARDDCVVIEDIERVGEADAVLVSTRLPRVQLQSLVTELRPRTKKPIIALAHAG
                     GEQSAVEVMRSGGSAVVAEGNEHAVRALLAGEETPASLTETYDAHMGRRRSRDLGGRG
                     RDPFTNLPTLSAFEGRLREFEQSEEPVRIGFLRILNFDHAARRLATEATGILRRRLAM
                     QYAELARENGVELFAVGPSDFAFLAPGMTGPEAQFFGRDLARVTEAFAPTAHGPLGLA
                     VGHAGPELSSEIGSLRELAQRAVALAAVQEDSVVIGAEELSRTLTSTTELEVALRMLQ
                     AIERQDPAGEGHGARVALRAAELAHKLGFEGHERAQIRFAAHLHDLGKIGLPVSAMHL
                     PEELDDEQAKLYRTHPSRGAEYLLVSAGKEVAAAVAAHHEHWDGTGFPYKLAGQDIPV
                     AARIICVADAWDRWAGGQEGDVAATELMERLREGAGTRFDPAVVDAAVEILSSVAVQQ
                     ADEARAAGVTS"
     gene            1561..2223
                     /locus_tag="VNU01_01020"
     CDS             1561..2223
                     /locus_tag="VNU01_01020"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH31230.1"
                     /translation="MEHVHAVQARIATIRGLVESMRPDSGGAGRFHAALTDALAEHGA
                     GASGSGVQKAAPGEPVMSAASLGAARFTPGAQGAGGGGLLMPVDARLSSPFGPRVHPV
                     TGRRHDHAGVDFAAPAGTPIRAAAGGTVAFAGVRGGYGNLVILDHGDGMQTWYAHQRD
                     VAVMEGQRVPAGGILGTVGSTGRSTGPHLHLEVRRDGEPVDPLPLLTRAHGIGTGHGA
                     RR"
     gene            2281..2769
                     /gene="flgN"
                     /locus_tag="VNU01_01025"
     CDS             2281..2769
                     /gene="flgN"
                     /locus_tag="VNU01_01025"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HWH31231.1"
                     /translation="MDAVNELRRILDRESVVVELVLFRLKAMELLLSAGEHRFLNDAA
                     EELDQAVDRLEALETMRGLSVEELAQALGMPVEECALTDVLRLADERQRGELVTSQVR
                     LSGLLRELEGQTALARDLALARVAAVRAALERLSRVPGSRYGRDGIVPGRGGNRSFDE
                     RL"
     gene            complement(2772..3152)
                     /gene="csrA"
                     /locus_tag="VNU01_01030"
     CDS             complement(2772..3152)
                     /gene="csrA"
                     /locus_tag="VNU01_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HWH31232.1"
                     /translation="MLVLTRRTGESIVIGNNIVVTVLEVRHDQVRIGIDAPRTVQVHR
                     EEVFNQVLRENTAAVASAADRANPLLRKPLPRPAAAQPVRKGPRLEPKRVAPRPPDAA
                     EPTAGPPGAPPPPSAGPDDAPSPS"
     gene            complement(3155..3583)
                     /locus_tag="VNU01_01035"
     CDS             complement(3155..3583)
                     /locus_tag="VNU01_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HWH31233.1"
                     /translation="MSGSASGTATAPEVAEDQILWFPDGIPGFPYSHRFVLVEMAPDS
                     PFQILQSADEPDVSLIVTVPWLFFPDYDFELSELEQRELGITSEEETVVFSAVTLDGE
                     HHRVYLNLLGPIVVNTTNRRGRQVVLVDSDLPVRAPVELG"
     gene            complement(3622..4509)
                     /locus_tag="VNU01_01040"
     CDS             complement(3622..4509)
                     /locus_tag="VNU01_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWH31234.1"
                     /translation="MRVTQEGMVQNTLLRLQERIGRLDEAGRRLSSGKIVQVASDDVS
                     GMNRVLSLRARIRSREQEERNAADGLTLLNIADSKLQTVNTRLLRVRELTVRAANTTD
                     PAARESLAKEIEAIRDDIASVANTSIDGRMLFAGTANVPQAVTVPGFAYAGDYNAIQR
                     RVSDEDLVTVNVNAGELFGFDLPAGQDLFSQLDGLAADIRTSNGTTVSARLDELDAGV
                     ARINRGLAAVGAATNRIDAAMVRNSDEQLALKGELAKVEDVDLAEAIMDLQTQEVAYQ
                     ATLAALSRVLQPSLVDFLR"
     gene            complement(4530..5894)
                     /gene="flgK"
                     /locus_tag="VNU01_01045"
     CDS             complement(4530..5894)
                     /gene="flgK"
                     /locus_tag="VNU01_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HWH31235.1"
                     /translation="MTLSTALTALRAAQTGIDVTSHNIANANTAGFTRQRVSLTTNTP
                     YDSAVGPMGTGVGVEEIARIRDTFLDMRLHASNSALGSMEVKADLLARTEALLGEPDF
                     GITKELGEVWATFDELSLRPTDKSVRLTVLSALDTLASRTRTIATSLDALGDDVRLQL
                     NDSVNKANDQIQQLADLNKMIASLQGNGGGAPNDLLDRRDLLVDQLSRSLGASASYAE
                     DGSTRLNLNGFELVSGQRANLLVLQPDNSLLHPSGSTVPGGGRIGGLQAFLVTPGGLL
                     EGVKQQLNDFVTELVTQVNMQHAAGVDLNGAAPAAPPAPQLLELTGGADTLAVGITDP
                     DLLAAASPGMGQLDGSNAMALAQLRTKPAAVGLPVIDEAARGLITDLGAQVASARRQA
                     AAEGGLNSAAASARRDHNAVSLDEEMVGLVTYQRSYEAAARVATTIDEMLQTVVSRLG
                     VVGR"
     gene            complement(5920..6441)
                     /locus_tag="VNU01_01050"
     CDS             complement(5920..6441)
                     /locus_tag="VNU01_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HWH31236.1"
                     /translation="MADTGPGPSRERLEALADTLWAERHVVEFLLYKLTVAKLLLAAD
                     ERRFVTAALSEVEQVVGRLREAEERRQAALADVAAEWRRPAAEVTLSALAKETSEPMR
                     GVFEDHRKGFQELSVEIEETAAENRRLASGSLNSIQEALGALTGGNPAPTYTAKGRTQ
                     SPTVGPTQWDRSL"
     gene            6596..7804
                     /locus_tag="VNU01_01055"
     CDS             6596..7804
                     /locus_tag="VNU01_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWH31237.1"
                     /translation="MPRVSAKASQFTESVIREMTRLAAVHSTPGRPVVNLAQGFPDFP
                     APPEVKEAARRAIAEDVNQYAVTWGAPVLREAIAADALRHHGWRLDPERELTVTCGAT
                     EGMIAALMAVLDPGDELLVFEPFYENYGADAILSGATPVHVPLDPPTLERPSFALDPE
                     KVRAAITPRTRGIVVNSPNNPTGLVLERTELEALRDLCVEFDLVAFTDEIYEHIVYDG
                     ARHIPLATIDGMAERTCTVNALSKTFSVTGWRVGWVVAPPALTAAVRKVHDFLTVGAA
                     APLQQAGAVALGLPDDYFAGLAAFYQARRDRMLEILRGVGLEPVVPRGAYYTLADCTP
                     FGMDDAKFAERLVAELGVACVPGSSFFSREPDPGLRGHAPRYVRFTFCKTMDTLDAAA
                     ERLAGLEAWR"
     gene            7801..8661
                     /locus_tag="VNU01_01060"
     CDS             7801..8661
                     /locus_tag="VNU01_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31238.1"
                     /translation="MSATVYAVANQKGGVAKTTTTLALAAALAERGLAVLAVDLDPQA
                     CLTFSLGYDPDELDPTVHDVLTGRAPLPKSVLQHAETDVAPANIDLAGAEVALLTRTG
                     REYVLRAELEPLRETYDAILLDCPPSLGVLTLNGLTAADRVVIPVQCETLSHRGVAQL
                     LETIHDVQRLTNRGLEVAGVVATMFDGRTRHSREVLADVQQRYGLRLLGVPVRRSVRF
                     AEAHHEGKSILAHDGGVPGANAYRVIAAELAGLDVDDAVREAAGAVPAGVVVAPRDSH
                     ATEDAPGEQS"
     gene            8661..8963
                     /locus_tag="VNU01_01065"
     CDS             8661..8963
                     /locus_tag="VNU01_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31239.1"
                     /translation="MGFDRIKPPQQRLLERDAAPDPEGRAALFTGGGDRPATGAITDG
                     LVLECSRCGAASALDPAMAVRAALPLFLLAPWRSHPLFAVCPTGRHRAWLRITRAE"
     gene            8960..9742
                     /locus_tag="VNU01_01070"
     CDS             8960..9742
                     /locus_tag="VNU01_01070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWH31240.1"
                     /translation="MSPGARELAALAAKAATEAGRELLRRRGQVHGLDTKTSATDPVT
                     DADRASERLLVEILLGERPQDGLLGEEGASRDGTSGLRWVLDPLDGTVNWLYGFPAWC
                     VSVACEDGASEPGGALAGAVYDALRDELYWAARGEGAWLRDERLRVNDPVPLERALIA
                     TGFAYDPGDRRRQAQTVARVIPAARDVRRGGSAALDLCWVAAGRLDGYYEDTTSRWDW
                     AAAALIAREAGAVVTPFGGGSRDGGVVAAGPALHDELRALVT"
     gene            complement(9817..>10105)
                     /locus_tag="VNU01_01075"
     CDS             complement(9817..>10105)
                     /locus_tag="VNU01_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31241.1"
                     /translation="KREPQQKREPERGESARGGAARPGSGSRSGAGRSRTSSDRAGRG
                     GRDRRGGAAGRDPRTARDRDRRRDLPDVDPEVARDGARPVISRPLKVEHLP"
BASE COUNT         1249 a         3630 c         3808 g         1418 t
ORIGIN      
        1 cgcggcggaa ggcgaccccg gcgccgtcgt cgaccccggt gaggagctga tcggcgcgcc
       61 cgagcccgag cccgcgcccg agtccgccgt gcgcttggac gcgaccctgt tcatggcggg
      121 cgtgccggac tcttcgcgcc agcaggtcgc gcgcgacgac tgcgtcgtca tcgaggacat
      181 cgagcgcgtc ggggaggccg acgccgtgct ggtgtccacc cggctcccgc gcgtccagct
      241 gcagagcctg gtcaccgagc tgcgtccgcg caccaagaag ccgatcatcg cgctcgccca
      301 cgccggcggg gagcagtccg cggtggaggt catgcgctcg ggcggctccg cggtggtggc
      361 cgagggcaac gagcatgcgg tgcgtgccct gctcgccggc gaggagacac cggcgtcgct
      421 caccgagacg tatgacgcgc acatgggccg ccggcgctcg cgggacctcg gggggcgggg
      481 ccgggacccg ttcacgaatc tgcccacgct gtcggcgttc gagggccggc tgcgcgagtt
      541 cgagcagtcc gaggaacccg tccggatcgg cttcctgagg atcctgaact tcgaccacgc
      601 cgcccggcgc ctggcgacgg aggccaccgg catcctgcga cgccgcctgg ccatgcagta
      661 cgccgagctc gcgcgcgaga acggcgtgga gctgttcgcc gtgggtccgt ccgacttcgc
      721 gttcctcgct ccgggaatga ccggccctga ggcgcagttc ttcggccgcg acctggcgcg
      781 cgtcacggag gcgttcgcgc ccaccgcgca cgggccgctg gggctggccg tcggccacgc
      841 cggcccggag ctgtccagtg agatcggcag cctccgggaa ctcgcgcagc gcgccgtcgc
      901 gctcgccgcc gtccaggagg acagcgtggt gatcggcgcg gaggaactgt cccggacgct
      961 gacgtccacc accgagctcg aggttgcgct gcgcatgctg caggcgatcg agcgccagga
     1021 cccggccggg gaggggcacg gcgcccgggt ggcgctgcgc gccgccgagc tcgcgcacaa
     1081 gctcggcttc gaggggcacg agcgcgccca gatccggttc gccgcccatc tgcacgatct
     1141 cggcaagatc ggcctgcccg tctcggcgat gcacctgccg gaggagctgg acgacgagca
     1201 ggccaagctc taccgcacgc acccgtcgcg cggcgccgag tacctgctgg tctccgccgg
     1261 caaggaggtc gcggccgccg tcgccgccca tcacgagcat tgggacggca ccggattccc
     1321 ctacaagctc gcgggccagg acatccccgt ggcggcgcgc atcatctgcg tcgccgacgc
     1381 gtgggaccgc tgggccggcg gccaggaggg cgacgtggcc gccacggagc tgatggaacg
     1441 gctgcgggag ggggccggga cgcgcttcga ccccgccgtg gtcgacgccg cggtggagat
     1501 cctgtcctcg gtggccgtgc agcaggccga cgaggcgcgc gccgccggcg tcacgagctg
     1561 atggagcacg tgcacgcggt ccaggcgcgg atcgcgacca tacgcggcct cgtggagtcg
     1621 atgcgccctg acagcggtgg cgcgggccgt ttccacgccg cgctcacgga cgcgctcgcc
     1681 gagcacggag ccggcgcttc tggcagcggc gtgcagaagg ccgcgccggg agagcctgtg
     1741 atgtcggcgg cgtccctggg cgccgcccgg ttcacgcccg gggcgcaggg cgcgggcggt
     1801 ggcgggctcc tgatgccggt ggacgcgcgc ctgtcctcgc cgttcggccc gcgcgtgcat
     1861 ccggtcaccg gccggcgcca cgaccacgcc ggcgtggact tcgccgcgcc ggccggcaca
     1921 ccgatccgcg ccgcggccgg cggcacggtc gcgttcgccg gcgtgcgggg cggctacggc
     1981 aacctggtga tcctcgatca cggcgacggc atgcagacct ggtacgcgca ccagcgcgac
     2041 gtggcggtca tggagggcca gcgcgtcccg gccggcggga tcctggggac cgtgggctcc
     2101 accggccgtt ccacggggcc gcacctgcac ctggaggtgc gccgcgacgg tgagcccgtc
     2161 gaccccctgc ccctgctgac gcgcgcgcac ggcatcggga ccggccacgg cgctcgccgg
     2221 taagctgccc agggcggccc tgcgacggcc gcgggcaggc gggggaccgg agcgggagcc
     2281 gtggacgcgg tcaacgagtt gagacgcatc ctcgaccgtg agtcggtggt cgtcgagctc
     2341 gtgctgttcc ggctcaaggc gatggagctg ctcctgtccg ccggcgagca ccggtttctc
     2401 aacgacgccg ccgaggagct ggatcaggcg gtggaccggc tggaggcgct cgagaccatg
     2461 cgcgggctgt ccgtggagga gcttgcccag gccctcggga tgccggtcga ggagtgtgcg
     2521 ctgaccgatg tcctgcgcct ggcggacgag cgccagcgcg gcgagctggt cacctcccag
     2581 gtgcggctct ccggcctgct gcgcgagctg gagggccaga ccgcgttggc gcgggacctt
     2641 gccctggcac gcgtggccgc ggtgcgcgcg gcactggaaa ggctcagccg cgtgcccggc
     2701 tctcggtacg gccgggacgg gatcgtgccg ggacgtggcg gcaaccggtc attcgacgag
     2761 cgactgtgac gtcagctcgg cgacggcgcg tcgtccgggc cggctgaggg cggcgggggc
     2821 gcgccaggcg gcccggcggt cggttctgcg gcgtccggcg gcctcggcgc cacgcgcttc
     2881 ggttccagcc tgggcccctt gcggaccggt tgcgcggcgg ccggccgggg cagcggcttg
     2941 cgcagcagcg ggttcgcgcg gtcggctgcg ctggccacgg cggcggtgtt ctcccgcagc
     3001 acctggttga agacctcttc gcggtggacc tggacggtgc gcggcgcgtc gatgccgatg
     3061 cgcacctggt cgtggcgtac ttccagcacg gtcacgacga tgttgttgcc gatgacgatg
     3121 gactcgccgg tgcggcgtgt gaggaccagc atgcctatcc cagctcgacg ggcgcgcgga
     3181 cggggaggtc gctgtccacc agcacgacct gcctgccgcg ccggttcgtg gtgttgacca
     3241 cgatcgggcc gagcaggttc aggtagacgc ggtggtgctc gccgtccagg gtgacggcgc
     3301 tgaacactac cgtctcctcc tcggaggtga tgcccagttc gcgctgctcc agctcggaca
     3361 gttcgaagtc gtagtccggg aagaacagcc acggcaccgt gacgatcagc gacacatccg
     3421 gctcgtcggc gctctgcagg atctgaaatg ggctgtccgg cgccatctcc acgagcacga
     3481 aacggtggga gtacggaaaa ccggggatgc cgtcggggaa ccagaggatc tggtcctcgg
     3541 cgacctcggg ggcggtggcc gtcccgcttg cggaaccact catctcgtgc ggacctcgtc
     3601 ggacgtcgga atggcgggcg ctcagcgcag gaagtcgacc agggagggct gcaggacccg
     3661 ggacagcgcc gccagcgtcg cctggtaggc gacctcttgg gtctgcaggt ccatgatcgc
     3721 ctcggcgagg tccacgtcct ccaccttggc cagctctccc ttcagtgcca gctgctcgtc
     3781 gctgttgcgc accatagcgg catcgatgcg gttcgtcgcg gcgccgaccg cggccaggcc
     3841 ccggttgatg cgggccacgc cggcgtccag ctcgtccagg cgggccgaga cggtggtgcc
     3901 gttgctcgtg cggatgtccg ccgccaggcc gtcgagctgg ctgaacaggt cctggccggc
     3961 gggcaggtcg aagccgaaca gctcgcccgc gttcacgttg acggtcacga gatcctcgtc
     4021 gctgacgcgc cgctggatcg cgttgtagtc gccggcgtac gcgaagcccg ggacggtgac
     4081 cgcctgcggg acattcgcgg tgcccgcgaa cagcatgcgc ccgtcgatgg aggtgttggc
     4141 caccgacgcg atgtcgtcgc ggatcgcctc gatctccttg gcgagcgact ccctggctgc
     4201 cggatcggtg gtgttcgcgg cgcgcacggt gagctcacgc acccgcagca gccgcgtgtt
     4261 cacggtctgc agcttgctgt cggcaatgtt cagcagggtg agcccgtcgg ccgcgttgcg
     4321 ctcttcctgc tcgcgcgacc ggatccgggc gcgcaggctg agcacgcggt tcatgccgga
     4381 gacgtcgtcg gaggccacct gcacgatctt gcccgacgac agccggcggc cggcctcgtc
     4441 gaggcgcccg atccgctcct gcagccgcag cagcgtgttc tgcaccatgc cctcttgcgt
     4501 cacgcgcacg tcgccgtctc ctgtccctct taccggccga ccacgccgag gcggctgacc
     4561 acggtctgga gcatctcgtc gatggtggtc gccacgcgcg cggcggcctc gtagctgcgc
     4621 tggtacgtca ccagtcccac catctcctcg tccagcgaca ccgcgttgtg gtcgcggcgg
     4681 gcggacgcgg ccgcgctgtt gaggcccccc tccgccgcgg cttggcgtcg cgccgaggcg
     4741 acttgcgcgc ccagatccgt gatgagcccc cgcgcggcct cgtcgatcac gggaagcccc
     4801 accgcggccg gcttggtgcg cagctgggcg agcgccatgg cgttgctgcc atcgagctgg
     4861 cccatccccg ggctcgccgc cgcgagcagg tcggggtccg tgatgcccac cgcgagggtg
     4921 tccgcgccgc cagtcagctc cagcagctgc ggcgccgggg gggcggcggg agcggcgccg
     4981 ttgaggtcca cgcccgcggc gtgctgcatg ttgacctgtg tcaccagttc cgtcacgaag
     5041 tcgttcagct gctgtttcac gccctccagc aggccgccgg gggtgaccag gaacgcctgc
     5101 agccccccga tccggccgcc gccggggacg gtgctgccgg acgggtgcag cagcgagttg
     5161 tccggctgga gcacgagcag gttggcgcgc tgcccgctca ccagctcgaa gccgttcagg
     5221 ttgagccgcg tgctgccgtc ctccgcgtac gaggcggacg cgcccaggct ccgcgagagc
     5281 tggtcgacga gcaggtcgcg gcggtccagc aggtcgttcg gggcgccgcc gccgttgccc
     5341 tgcagcgacg cgatcatctt gttcagatcc gcgagctgct ggatctggtc gttggccttg
     5401 ttgaccgagt cgttgagctg gaggcgcacg tcgtcgccca aggcgtccaa gctggtggcg
     5461 atggtgcgcg tgcggcttgc gagggtgtcc agcgccgaca gcaccgtgag ccgcaccgac
     5521 ttgtccgtgg gccgcagcga cagctcgtcg aaggtcgccc acacctcgcc gagctccttg
     5581 gtgatcccga agtccggttc ccccagcagc gcctcggtcc gggccagcag gtccgccttg
     5641 acctccatgg aacccagcgc cgagttgctc gcgtgcagcc gcatgtccag gaacgtgtcc
     5701 ctgatccgtg cgatctcctc caccccgacc ccggtgccca tggggcccac ggcactgtcg
     5761 tagggcgtgt tggtggtgag tgacacgcgc tggcgggtga agccggccgt gttcgcgttc
     5821 gcgatgttgt gggacgtgac gtcgataccg gtctgcgcgg cgcgcagcgc cgtgagcgcc
     5881 gtcgaaagcg tcacgaagtt gctcatcgcg cggccgccgt cacagggagc ggtcccactg
     5941 ggtcgggccc acggtgggcg actgcgtgcg gcccttcgcg gtgtacgtcg gggccgggtt
     6001 cccgccggtc agcgcgccca gcgcctcctg gatggagttc agcgagccgc tggcgagccg
     6061 ccggttctcc gcggcggtct cctcgatctc cacggacagc tcctggaagc ccttgcggtg
     6121 gtcctcgaag acgccgcgca tcggctcgga ggtctccttg gcgagcgcgc tgagcgtcac
     6181 ctccgccgcg ggacggcgcc attccgccgc gacgtccgcg agcgcggcct gccggcgctc
     6241 ctccgcctcg cggaggcggc ccaccacctg ctccacctcg gagagggcag cggtgacgaa
     6301 ccggcgctcg tcggcggcca gcagaagctt cgccacggtc agcttgtaga gcaggaactc
     6361 caccacgtgg cgttccgccc acagcgtgtc ggccagcgcc tcgaggcgct cgcggctggg
     6421 cccaggtcct gtgtcggcca tggtgttccc gtcggctcgc gcggctttcc cttaagcacg
     6481 atctgtgtcc gaatcgcacc aagcgtaagg ttctgcgcgg gcgttcgctt tcacgcttcc
     6541 gcctgtcccg acgggcagaa cccccgcccg gccctgccgg agtagccttc gccgcatgcc
     6601 ccgggtcagc gccaaggcgt cccagttcac cgagtcggtc atccgcgaga tgacgcggct
     6661 ggccgcggtg cactccacgc cgggacgccc ggtggtcaac ctggcgcagg gcttcccgga
     6721 cttcccggcc cccccggagg tcaaggaggc ggcccggcgg gccatcgccg aggacgtcaa
     6781 ccagtacgcg gtgacgtggg gcgcccccgt cctgcgcgag gcgatcgcgg ccgacgcgct
     6841 gcgccaccac ggctggcggc tggacccgga gcgcgagctc accgtcacct gcggcgccac
     6901 cgagggcatg atcgccgcgc tgatggcggt cctggacccc ggcgacgagc tgctcgtgtt
     6961 cgagccgttc tacgagaact acggggcgga cgcgatcctg tccggcgcca caccggtgca
     7021 cgtgccgctg gatccgccca cgcttgaacg cccgtcgttc gcgctggacc cggagaaggt
     7081 ccgcgccgcg atcaccccgc gcacgcgcgg catcgtggtg aacagcccca acaaccccac
     7141 cggcctggtg ctggagcgca ccgagctgga ggcgctgcgc gatctgtgcg tggagttcga
     7201 cctggtggcg ttcaccgacg agatctacga gcacatcgtc tacgacggcg cccggcacat
     7261 cccgctggcc acgatcgacg gcatggccga gcgcacctgc accgtcaacg cgctgtccaa
     7321 gacgttctcg gtcaccggct ggcgcgtggg ctgggtggtc gcgccgccgg cgctcaccgc
     7381 cgccgtgcgc aaggtgcacg acttcctcac cgtcggcgcc gcggcgccgc tgcagcaggc
     7441 cggcgcggtg gccctgggcc tgcccgacga ctacttcgcc gggctggcgg cgttctacca
     7501 ggcgcgccgc gaccgcatgc tggagatcct gcgcggggtg ggcctggagc cggtggtgcc
     7561 ccgtggcgcc tactacacgc tggcggactg caccccgttc ggcatggacg acgcgaagtt
     7621 cgccgagcgg ctggtggccg agctcggcgt ggcgtgcgtg cccggcagct cgttcttctc
     7681 gcgggagccg gacccgggcc tgcgcggcca cgcgccgcgc tacgtgcgct tcacgttctg
     7741 caagaccatg gacaccctgg acgcggcggc ggagcggctg gccggactgg aggcgtggcg
     7801 atgagcgcca ccgtgtacgc cgtcgccaac cagaagggcg gcgtggccaa gaccaccacg
     7861 accctggcgt tggccgcggc gctggccgag cgcggcctgg ccgtgctggc cgtggacctg
     7921 gacccgcagg cgtgcctgac gttctccctg ggctacgacc cggacgagct ggaccccacg
     7981 gtgcacgacg tgctgacggg ccgggcgccg ctgcccaaga gcgtgctgca gcacgccgag
     8041 accgacgtcg cgccggccaa catcgacctg gccggcgccg aggtggcgct gctgacgcgc
     8101 accggccgcg agtacgtgct gcgcgccgag ctcgagccgc tgcgcgagac ctacgacgcg
     8161 atcctgctgg actgcccgcc gtcgctgggc gtgctgacgc tgaacggcct caccgccgcg
     8221 gaccgggtgg tgatcccggt gcagtgcgag acgctgtcgc accgaggcgt ggcgcaactg
     8281 ctggagacga tccacgacgt gcagcggctg accaaccgcg gcctggaggt ggccggcgtg
     8341 gtcgccacca tgttcgacgg gcgcacgcgc cacagccgcg aggtgctggc ggacgtgcag
     8401 cagcgctacg ggctgcgcct gctgggcgtg ccggtgcgcc gcagcgtgcg cttcgccgag
     8461 gcgcaccacg agggcaagtc gatcctggcc cacgacggcg gcgtgccggg cgccaacgcc
     8521 taccgggtga tcgccgcgga actggcgggg ctggacgtgg acgacgcggt gcgcgaggcg
     8581 gccggcgccg tgccggcggg cgtcgtcgtg gcgccgcgcg actcgcatgc gactgaggac
     8641 gcgccagggg agcagagctg atgggcttcg accggatcaa gccgccgcag cagcggctgc
     8701 tggagcgcga cgcggcgccg gacccggaag ggcgcgcggc gctgttcacc ggaggcgggg
     8761 atcgtccggc cacgggcgcc atcacggacg ggctggtgct ggagtgctcc cgctgcggcg
     8821 ccgcgtcggc gctggacccg gccatggcgg tgcgggcggc gctgccgctg ttcctgctgg
     8881 cgccgtggcg gtcgcacccg ctgttcgcgg tgtgccccac ggggcgccac cgcgcctggc
     8941 tgcggatcac gcgggccgag tgagcccggg cgcccgcgag ctggccgcgc tggcggccaa
     9001 ggcggccacc gaagccgggc gcgagctgct gcgccggcgg ggccaggtgc acggcctgga
     9061 caccaagacc agcgccaccg acccggtgac agacgccgac cgcgcgtccg aacggctgct
     9121 ggtcgagatc cttctcggcg agcgcccgca ggacggcctg ctcggcgagg agggcgcgtc
     9181 ccgggacggc acgtccggcc tgcgctgggt gctggacccg ctggacggca ccgtgaactg
     9241 gctgtacggc ttcccggcgt ggtgcgtgag cgtggcgtgc gaggacggcg cgtccgagcc
     9301 cggcggcgcc ctggccggcg cggtctacga cgcgctgcgc gacgagctgt actgggcggc
     9361 gcggggcgag ggcgcctggc tgcgcgacga gcggctgcgg gtcaacgacc cggtgccgct
     9421 ggagcgggcg ctgatcgcca ccgggttcgc ctacgacccc ggcgaccgcc gccggcaggc
     9481 gcagacggtg gcgcgtgtga tcccggccgc gcgggacgtg cgccgcggcg gctcggccgc
     9541 gctggacctg tgctgggtcg ccgcgggccg cctggacggc tactacgagg acaccacgtc
     9601 gcgctgggac tgggcggcgg cggcgctgat cgcgcgcgag gcgggcgcgg tggtgacgcc
     9661 attcggcggc ggctcccggg acggcggcgt ggtcgccgcg ggcccggcgc tgcacgacga
     9721 actgcgcgcc ctcgtcacct gacgcacccg tttccccgtt caaacgggtg cgggcaccgc
     9781 gatatcccgc agtgcccgca cccgctcgaa tggggactac gggaggtgct cgaccttcag
     9841 ggggcggctg atcaccgggc gggcgccgtc gcgcgcgacc tcgggatcga catccggcag
     9901 atcgcggcgg cgatcgcggt cgcgggccgt gcgcgggtcg cggccggcgg cgccaccacg
     9961 gcggtcgcga ccgcctcggc cggcacggtc cgacgacgtc cgtgagcggc cggcgccgga
    10021 ccggctgccc gagcccgggc gggcggcgcc gccacgcgcg gactcgccgc gctcgggctc
    10081 gcgcttctgc tgcggctcgc gcttc
//
LOCUS       DATMKC010000039         2539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_95049, whole genome shotgun sequence.
ACCESSION   DATMKC010000039 DATMKC010000000
VERSION     DATMKC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95049"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2539
                     /locus_tag="VNU01_01080"
     CDS             <1..>2539
                     /locus_tag="VNU01_01080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH31242.1"
                     /translation="GQHDRQLEVLYVSTAGMMIRLDALRTLGGFDARFPAFREDLDLC
                     WRAWLSGRRVAVVPSALGYHIAAGSRLARPFGKGRPWEARYLAERHTLAALLKNYSAA
                     SLAWVLPLVLLLALGKTSAFLVTRRFGDAIAVLRAYAWNVLQLGRTLRRRRIVQSRRQ
                     AGDAQLSHLFAPGLPRARAYAEALGNWLAGGSTRALLDETDGAEAEADEATALMRLLR
                     RHPAALTAAALLVVYVLGLLRFMGDGQLVGGDILPWPASPWEFLRSYASPWNGEPAGS
                     GAFASPVQVLLGFLSMLGMGSAWLAQRLVVFGLLPLGWALALRAGRLVTASVWPRILG
                     ATLYVVSPVLLGALAQGRLGVLLLGVLAPGLVLLAARAADPATATATAWRSAALLSLG
                     LTAAVAASPIAALPLAIAYGALLAVALRRRAVARQPALCLGMAGLAAFALLSPWLVGV
                     LREGGGLGRVTGNPALPLWRALAGVPEVVPGLGGLAGVVIAASAAGLVAAAVILGLQA
                     RPAAVSGLVAVVVVSGSAAWLVTRLGAQLVWTPALLLPAALALAGLAVMAAHYLGGGL
                     RQHAFGPRQLAVLLAAGMLTLGLVGGVARMAGGPWHGLTLDAQVVPAFVTADRFDVGA
                     YRILLLEARDGAVWWDVVPAEGPTMTRFGTTPSRRLLDAIGQAVEGAAGGGDPRAGAR
                     LGLLNVRYLVLHGDEPDDGLVEALIRQPALEPLPSGGGRVFRVDSWLPRAAVLSPERA
                     QRLLQTGDPGTGREGVTPLRRDEPGVYRAETVPAGLVVVSEAASPVWRAASERGGLSR
                     RAVDPVNGFELPAPAQAVRVRAAGGASHAAVAVVQALLLLGVLSLALR"
BASE COUNT          192 a          962 c         1020 g          365 t
ORIGIN      
        1 gggccaacac gaccggcagc tggaggtcct gtacgtctcc accgccggga tgatgatccg
       61 cctggacgcg ctgcgcacgc tcggcggctt cgacgcgcgc ttccccgcgt tccgcgagga
      121 cctggacctg tgctggcgcg cgtggttgtc cggccgccgc gtcgccgtgg tgcccagcgc
      181 gctcggttac cacatcgccg ccggcagccg cctggcgcgg cccttcggca agggccgccc
      241 ctgggaggcg cgctacctcg ccgagcggca cacgctcgcg gcgctgctga agaactactc
      301 ggccgcgagc ctcgcctggg tgctgcccct ggtcctgctg ctggccctgg gcaagacctc
      361 ggcgttcctg gtgacgcggc gcttcggcga cgccatcgcc gtcctgcgcg cgtacgcgtg
      421 gaacgtcctg cagctcggcc gcacgctgcg ccgccggcgc atcgtgcagt cccgccggca
      481 ggccggcgac gcgcagctgt cccacctgtt cgcgcccggg ctgccgcgcg cccgcgcgta
      541 cgccgaggcg ctgggcaact ggctggccgg cggcagcacc cgcgcgctgc tggacgagac
      601 cgacggcgcc gaggccgagg ccgacgaggc caccgcgctg atgcgcctgc tgcggcgcca
      661 tcccgcggcg ctgaccgccg cggcgctgct ggtggtctac gtgctgggcc tgctgcgctt
      721 catgggcgac ggccagctcg tgggcggcga catcctgccg tggccggcct cgccgtggga
      781 gttcctgcgt tcctacgcca gcccgtggaa cggcgagccc gccgggtccg gcgcgttcgc
      841 ctcgccggtg caggtgctgt tgggcttcct gtcgatgctc ggcatgggca gcgcctggct
      901 ggcgcagcgc ctggtcgtgt tcgggctgct gccactgggc tgggcgctgg cgctgcgcgc
      961 cgggcggctg gtcaccgcgt cggtgtggcc gcgcatcctg ggcgccacgc tctacgtcgt
     1021 ctcgcccgtg ctcctgggcg ccctggcgca ggggcgcctg ggcgtgctgc tgctgggcgt
     1081 gctggcgccc gggctggtcc tgctggcggc gcgcgccgcc gacccggcca ccgccaccgc
     1141 caccgcgtgg cgcagcgccg cgctgctcag cctgggcctc accgccgccg tcgccgcctc
     1201 gccgatcgcc gcgctgcccc tggcgatcgc ctacggcgcg ctgctcgccg tggcgctgcg
     1261 ccgccgcgcg gtggcgcgcc agcccgcgct gtgcctgggc atggcgggcc tggccgcgtt
     1321 cgcgctgctg tcgccgtggc tggtcggcgt cctgcgcgag ggcggcggcc tggggcgcgt
     1381 gaccggcaac ccggcgctgc cgctgtggcg cgcgctggcc ggcgtcccgg aggtcgtgcc
     1441 cgggctgggc gggctggccg gcgtggtcat cgccgccagc gccgcgggcc tggtcgccgc
     1501 ggcggtgatc ctgggcctgc aggcccggcc ggccgccgtc agcggcctgg tggccgtggt
     1561 ggtggtctcc ggatcggcgg cgtggctggt gacccgcctg ggcgcgcagc tggtgtggac
     1621 cccggcgctg ctgctgcccg cggccctggc gctggcgggc ctggcggtga tggccgccca
     1681 ctacctgggt ggcggcctgc gccagcacgc gttcggcccg cgccagctcg ccgtcctgtt
     1741 ggcggcgggc atgctgacgc tgggcctggt gggcggcgtg gcgcgcatgg cggggggacc
     1801 gtggcacggc ctgacgctgg acgcccaggt ggtgcccgcc ttcgtgacgg ccgaccgctt
     1861 cgacgtgggc gcctaccgca tcctgctgct ggaggcccgc gacggtgcgg tgtggtggga
     1921 cgtcgtgccc gccgagggac ccaccatgac gcgcttcggg accacgccgt cgcgccggct
     1981 gctcgacgcg atcggccagg cggtcgaggg cgccgcgggc ggcggcgacc cccgcgcggg
     2041 cgcgcgcctg ggcctgctca acgtgcgcta cctggtgctg cacggcgacg agcccgacga
     2101 cggcctggtg gaggcgctga tccgccagcc ggcgctggag ccgctgccgt ccggcggagg
     2161 ccgggtgttc cgcgtcgact cgtggctgcc gcgcgccgcc gtgctgtcgc cggagcgggc
     2221 gcagcggctg ttgcagaccg gtgacccggg caccggtcgg gagggcgtca cgccgctgcg
     2281 gcgcgacgag cccggcgtct accgtgccga gacggtcccc gcgggcctcg tcgtggtgtc
     2341 cgaggctgcc tcaccggtgt ggcgggccgc cagcgagcga ggcggcctgt cgcggcgcgc
     2401 cgtcgacccg gtgaacggct tcgagctgcc ggcgcccgcg caggccgtcc gggtgcgggc
     2461 ggcgggcggc gcctcgcatg ccgccgtcgc ggtggtgcag gcgctgctgc tgctgggcgt
     2521 gctgtcgctg gcgctgcgc
//
LOCUS       DATMKC010000040         4513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_166129, whole genome shotgun sequence.
ACCESSION   DATMKC010000040 DATMKC010000000
VERSION     DATMKC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166129"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VNU01_01085"
     CDS             complement(<1..883)
                     /locus_tag="VNU01_01085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWH31243.1"
                     /translation="MPRRPELPALTGLRFAAAVAVVLVHLRGHLAALLPASAPLDPAL
                     SAGALGVEVFFVLSGFILAYHYLDAFPRVDARAYGRFLRHRLARIYPLHLATLALFAA
                     LIGGVALLGRPIANSEAYGLRDLAANLTLTQCWTLRDCGVSWNGPTWSLSAEWLAYLA
                     FPLLAVTVRRIPRGTPLLAAIGATVAAQLWASEVAVLNGGIGRITGTFLLGVLLARLH
                     TDGLPRLRWDAVAAWAAATASITAIALATAGRAPTPVVPLFGVLVLGLANAPDSRLSR
                     LLGAPRMRFWGQASYGLY"
     gene            complement(1003..1758)
                     /locus_tag="VNU01_01090"
     CDS             complement(1003..1758)
                     /locus_tag="VNU01_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31244.1"
                     /translation="MDDPVQLLVALAVAGMLGRAAAPAWRNRVLAIAVWRRIRPVHVL
                     GCVGLLAVVLATALTLMRFVPFASWGLGSLIGLDGNAVFAPLQEATELAEQARQPGAG
                     GAAAPAAPGPDWVLLGIAGGFLSLLLVLFPWLAYVEERVFRAGLEHADLKSQVKAAFR
                     FGIVHLVMLIPVGAALAIAVAGFVYGRIYRAAYRRALGAPPEFESPFGPRRIIETPAR
                     RARTAAVMTTTVWHTTFNSLIVLIVLAGFVLAG"
     gene            1868..2356
                     /locus_tag="VNU01_01095"
     CDS             1868..2356
                     /locus_tag="VNU01_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31245.1"
                     /translation="MAKAGTMLRRGAAEARARKAKVNAGMAVRDSAKAAAHAAAGLAQ
                     TVRDEAADRAGELSEATRRGSHEAMLRVGDWVMTPAMADRLGLKAHTRRAGIMGALLG
                     VAAGLLVGMMLGRRNGQPQQATWADPESLAPPLGRGQVDPAATAEAQREAAGDPHTTV
                     TA"
     gene            2434..2895
                     /locus_tag="VNU01_01100"
     CDS             2434..2895
                     /locus_tag="VNU01_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31246.1"
                     /translation="MHRTRFHSLALPAPVRAEVRSRLRRAHAGRPFSALDPRDTVRRV
                     AGIAGELGLTATVYRGVVDLRGAEVDHVWLDLHGRVVDAAFPLLEREFVELLRRFVAG
                     DLEPHEIAEAAAGFALDARVLGEFPEPIRYLGRPVWSTRRALEPPGSHRNS"
     gene            2979..3200
                     /locus_tag="VNU01_01105"
     CDS             2979..3200
                     /locus_tag="VNU01_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31247.1"
                     /translation="MEPAPRLTSIAGVARALIATVGVDDAVSILVGQFGWDVAFSALS
                     RLDCPEASVALWRSVELLGCDETDSDTVR"
     gene            complement(3212..3697)
                     /locus_tag="VNU01_01110"
     CDS             complement(3212..3697)
                     /locus_tag="VNU01_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31248.1"
                     /translation="MAAFPMAAYLPGALAEPVDEFTSDLVAARVTVGQLARWAQRYLV
                     SHHAEQAGSCPCGRARCALADIAGGQEACRLAGELAWLPVAETRDAPPSRAGTRPEEI
                     AHLYVQALRNSLGAVFYCQRVQHLMGDCWFGTEERSGEALCARTLAVTHVLWSARRTL
                     I"
     gene            complement(3797..>4513)
                     /locus_tag="VNU01_01115"
     CDS             complement(3797..>4513)
                     /locus_tag="VNU01_01115"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWH31249.1"
                     /translation="LAARLAARFWPGPLTLVLEAAAHVPAGVTAGLGTVAVRVPDHAV
                     ALALLAAAGVPVAAPSANRSGRPSPTTAAHVTADLGERVAAVVDGGPCRLGVESTVVD
                     ARGDVAVILREGSVTREELAVPVHDGTGASLAASPGTRHAHYRPACRVELAAPGRAAA
                     RASALAAGGTRVGLVATEAAPDPVVEIARFRDTEELAAGLYAWLRAAEEAVDVLVVEE
                     VAPHGLGRAVMDRLRRAAGA"
BASE COUNT          596 a         1673 c         1761 g          483 t
ORIGIN      
        1 tgtacagccc gtaggaggcc tggccccaga agcgcatgcg cggcgcgccc agcagccgtg
       61 acagccggct gtcgggggcg ttggccaggc ccaggaccag cacgccgaac agcggcacga
      121 ccggcgtcgg ggcgcgcccc gccgtggcca gcgcgatcgc ggtgatgctc gccgtggcgg
      181 cggcccacgc ggccaccgcg tcccagcgca gccgcggcag cccgtccgtg tgcagccgcg
      241 ccagcaggac gccgagcagg aacgtgccgg tgatgcggcc gatgccgccg ttcaggaccg
      301 ccacctcgct ggcccacagc tgcgcggcca cggtggcgcc gatggccgcc agcagcggcg
      361 tgccgcgggg gatgcgccgg accgtcacgg ccagcagcgg gaacgcgagg taggccagcc
      421 actcggcgct gagcgaccac gtcggcccgt tccagctcac cccgcagtcg cgcagcgtcc
      481 agcactgcgt gagcgtgagg ttggcggcca ggtcccgcag gccgtaggcc tcgctgttgg
      541 cgatcggccg ccccagcagg gccacgccgc cgatcagcgc ggcgaacagg gccagcgtcg
      601 ccaggtgcag cgggtagatg cgcgcgagcc ggtggcgcag gaagcgcccg tacgcgcgcg
      661 cgtcgacgcg ggggaacgcg tccaggtagt ggtaggccag gatgaagccg gacagcacga
      721 agaacacttc cacgcccagc gcgccggcgg acagcgccgg gtccagcggc gcgctggccg
      781 gcagcagggc ggccaggtgc ccgcgcaggt gcaccagcac cacggcgacg gccgcggcga
      841 aacgcagccc ggtgagggcc ggcagctccg gtctgcgtgg catggtgagg ggcgctcctt
      901 gcggggggac gcacatgatc ctcgccgatt cgcgcgcgct tggcacctcc ggcggccgtc
      961 cgggcggaca ggagtcggcg gcgggcgccg cggcggcggc gctcacccgg ccaggacgaa
     1021 gccggccagc acgatcagca cgatcagcga gttgaacgtc gtgtgccaca ccgtggtggt
     1081 catgaccgcc gccgtgcgcg cgcgccgcgc gggcgtctcg atgatgcggc gcgggccgaa
     1141 cgggctctcg aactccggcg gcgcgcccag cgcgcgccgg tacgccgccc ggtagatgcg
     1201 cccgtacacg aagccggcca cggcgatggc cagcgcggcg cccaccggga tcagcatgac
     1261 caggtgcacg atgccgaagc ggaacgccgc cttgacctgg cttttcaggt ccgcgtgctc
     1321 caggccggcg cggaacacgc gctcctccac gtaggccagc cacgggaaca gcaccagcag
     1381 cagcgacagg aagccgccgg cgatgcccag cagcacccag tcggggcccg gcgccgcggg
     1441 tgccgcggcg ccccccgccc ccggctggcg cgcctgctcg gccagctcgg tggcctcctg
     1501 cagcggcgcg aacacggcgt tgccgtccag gccgatcagg ctgcccagcc cccaggacgc
     1561 gaacggcacg aagcgcatca gcgtcagggc ggtggccagc accacggcca gaaggcccac
     1621 gcagcccagc acgtggaccg gtcggatgcg ccgccacacg gcgatggcga gcacgcggtt
     1681 gcgccacgcg ggagccgcgg cgcgcccgag catgcccgcc acggccagcg cgaccagcag
     1741 ctgcaccggg tcgtccatgc gtcccagtgt gtcgcctgca cggccgtggc ggcgggagcg
     1801 cgcgcggttt tcgcgggcgc ggcgccggga agaccggcac cgagcccacg acacaaggag
     1861 atgcgcgatg gcgaaggccg gaaccatgct gcggcgaggt gcggcggagg cgcgcgcccg
     1921 caaggccaag gtgaacgccg gcatggcggt ccgtgacagc gccaaggcgg ccgcccatgc
     1981 ggcggcgggg ctcgcgcaga ccgtgcgcga cgaggcggcg gaccgtgccg gggagctgtc
     2041 ggaggccacg cgccgcggca gccacgaggc gatgctgcgc gtgggcgact gggtcatgac
     2101 gcccgcgatg gccgaccggc tggggctgaa ggcgcacacc cgcagggccg ggatcatggg
     2161 cgcgctgctc ggcgtggccg cgggcctgct ggtcggcatg atgctggggc gccgcaacgg
     2221 gcagccgcag caggcgacgt gggcggaccc ggagagcctg gcgccgccgc tgggccgtgg
     2281 ccaggtggac cccgccgcga ccgccgaggc gcagcgcgag gcggccggcg acccgcacac
     2341 caccgtcacc gcctgagacg ccgcgcgcca ccagatggcc gcgcggtacc agatgcttga
     2401 cgtggggacc ggatctgccg acaactgcta ctgatgcacc ggacccggtt ccactcgctg
     2461 gcgctgcccg caccggtgcg ggcggaggtg cggtcgcgcc tgcggcgcgc ccacgccggc
     2521 cggccgttct ccgccctgga cccgcgcgac acggtgcgcc gggtggcggg catcgccggc
     2581 gagctggggc tcacggcgac cgtctaccgg ggtgtggtgg acctgcgcgg cgcggaggtc
     2641 gaccacgtct ggctggacct gcacggccgc gtcgtggacg ccgccttccc gctgctggag
     2701 cgcgagttcg tcgagttgct gcgccgcttc gtggcgggcg acctcgaacc gcacgagatc
     2761 gccgaggccg cggccggctt cgcgctggac gcgcgcgtcc tgggggagtt cccggagccg
     2821 atccgctacc tcggccggcc ggtgtggtcc acgcgccgcg cgctggagcc gccgggatcg
     2881 caccggaatt cttgatcagt cttttgtcag ggcgttactc acgcgtccgc ccgctcgttg
     2941 atgctttcga cagcacggac gagcaggagc gggagagcgt ggagccagca cctcggctga
     3001 ccagcatcgc gggcgtcgcc cgggcattga tcgccacggt cggcgtcgac gacgccgtga
     3061 gcatcctcgt ggggcaattc gggtgggatg tggcgttcag cgcgctgtcg cgcctggact
     3121 gcccggaggc cagcgtggcc ctgtggcggt cggtggagct gctcggctgc gacgagaccg
     3181 actccgacac cgtccggtag cgcggtcctc ttcagatgag ggtgcggcgg gcgctccaca
     3241 acacgtgggt caccgccagc gtgcgggcgc acagcgcctc gccggagcgc tcctcggtgc
     3301 cgaaccagca gtcgcccatg aggtgctgca cgcgctggca gtagaacacc gccccgaggg
     3361 agttgcgcag ggcctgcacg tacaggtggg cgatctcctc gggcctggtg cccgcgcgtg
     3421 acggcggcgc gtcccgcgtc tcggcgaccg gcagccacgc cagctcgccg gcgagccggc
     3481 acgcctcctg gccgcccgcg atgtcggcca gcgcgcagcg ggcgcggccg cacgggcacg
     3541 agcccgcctg ctcggcgtgg tggctgacca ggtagcgctg cgcccagcgg gcgagttggc
     3601 ccaccgtgac gcgggcggcc accaggtcgg aggtgaactc gtcgaccggc tcggccaggg
     3661 ctccgggcag gtaggccgcc atggggaatg ccgccatgac cgcctccttt cgcctggccc
     3721 gccgaccggg ccgcttccta ctgggtatgt cggccgatgg gcgccgtcac tgaaccggtt
     3781 gcccgatcgg gtgacgtcag gcgccggcgg cccgccgcag ccggtccatc acggcgcgcc
     3841 ccagcccgtg cggggcgacc tcctcgacga cgaggacgtc gacggcctcc tcggcggcgc
     3901 gcagccaggc gtacaggccg gcggccagtt cctccgtgtc gcggaagcgc gcgatctcca
     3961 cgacgggatc gggcgccgcc tcggtggcga ccaggccgac gcgcgtgccg ccggcggcga
     4021 gggccgacgc gcgcgccgcc gcccggcccg gcgcggcgag ctcgacgcgg caggccggcc
     4081 ggtagtgggc gtgccgggtg cccggcgacg ccgccaggga cgcgccggtg ccgtcgtgga
     4141 ccggcacggc cagctcctcc cgggtcaccg agccctcgcg caggatcacc gcgacgtcgc
     4201 cgcgcgcgtc cacgacggtg gactccacac ccagccggca cgggccgccg tccacgacgg
     4261 cggcgacgcg ctcgccgagg tccgcggtca cgtgggcggc cgtggtgggg ctgggccggc
     4321 cggagcggtt cgcgctcggc gcggccacgg gcacgccggc cgccgcgagc agcgccaggg
     4381 cgaccgcgtg gtccggcacg cggaccgcca ccgtgcccag cccggcggtc acgccggcgg
     4441 gcacgtgcgc cgcggcctcg agcaccaggg tgagggggcc gggccagaag cgcgccgcca
     4501 ggcgggcggc gag
//
LOCUS       DATMKC010000041         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_334680, whole genome shotgun sequence.
ACCESSION   DATMKC010000041 DATMKC010000000
VERSION     DATMKC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334680"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VNU01_01120"
     CDS             complement(<1..1895)
                     /locus_tag="VNU01_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31250.1"
                     /translation="MSSRTDPPQTLSPPALQARAVGHGPALAEAVAHTVAGLQDGDPL
                     RGVTVLVPTRSAGVALRRQVAGRLGGMAAVRFELAARAAELLAAPELAAAGKRPLRPA
                     VLTEAVRAVLHQAPGRFAPVADHPATARALARTVRDLGSVPAGALAGLSRARGREAAR
                     VAGAVRALLHDRYDEHDLLRTAASLAAERPAAAAELGAVVAIVDRALSPAATAFLRAV
                     AGQGPVTVLVALTGDERADAGALQAADALGCELGRAPQPAVADAAPDGTVRVLSAPDP
                     ETEVRAAVRRIAERFDAGAPLHRMALLWTADRPYALLVRDALAAAGLPSNGGPRTRLG
                     DSPTGRALLGMLALASGGPRPLAREAVAAWLAEAPLRREAGGAPVDGARWDAVSRRAG
                     VVAGAGQWRARLERYAATVERRADDEVRDGASDAVRRAAAAEAAHARALAGFVEELAQ
                     RLRAPAGGQWSAFARWARELLDRYVGEPRAGWGDDAVEADRRVRAALDGLAELDGVGA
                     SADLGAFRRAVEAELDGRLGRRGRLGEGLLTGPLREGLCQELDLVVVVGLVEGLAPPS
                     VRDDPVLPDRERAAVPGLPLRGDQPAERRRELLAALAAAPERLLCAARSDNRAGRAPP
                     RAPGRAAP"
     gene            2069..2617
                     /locus_tag="VNU01_01125"
     CDS             2069..2617
                     /locus_tag="VNU01_01125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH31251.1"
                     /translation="MTLDLPGVKRLIPLPEDGAELPNHTPRCMVCGRDNPEGWHLTVR
                     RHGDEVRCEFTFDRRHEGGPGLAHGGAVAAVCDDVLGHVLTLLGVPAVTRRLEVDYLK
                     PVLLGEPHTMAGRIESVDGRKVWLAMEALAPDGQPRFTARGLFIRVGLEHFLAGLSPE
                     ERERAQERLAELRERGEDVTAW"
BASE COUNT          286 a         1057 c         1043 g          252 t
ORIGIN      
        1 ggtgctgccc gaccgggagc gcgcggcggt gcccggccgg cccggttgtc gctgcgcgct
       61 gcgcacagca ggcgctcggg ggcggcggcc agcgccgcga gcagttccct gcgccgctcg
      121 gcgggctggt cgccgcgcag cggcaggccg ggcaccgccg cgcgctcccg gtcgggcagc
      181 accgggtcgt cgcgcacgct cggcggggcc agcccttcga ccagccccac caccacgacc
      241 aggtccagct cctggcacag gccttcgcgc agcggcccgg tcagcagccc ctcgcccagg
      301 cggccgcgcc gccccaggcg cccgtcgagc tcggcctcca cggcgcgccg gaacgcgccc
      361 agatccgcgc tcgcgccgac cccgtccagc tcggccaggc catccagggc cgcgcggacg
      421 cggcggtcgg cctccacggc gtcgtcgccc cagcccgcgc gcggctcgcc gacgtagcgg
      481 tccagcagct cgcgggccca ccgggcgaac gcgctccact ggccgccggc cggggcccgc
      541 agccgctgcg ccagctcctc gacgaagccg gcgagcgcgc gcgcgtgcgc ggcctccgcg
      601 gcggcggcgc ggcgcacggc gtcgctcgcg ccgtcgcgca cctcgtcgtc ggcgcgccgc
      661 tccaccgtgg ccgcgtagcg ctccaggcgc gcccgccact gcccggcccc ggccaccacg
      721 cccgcccggc gggacaccgc gtcccagcgc gccccgtcca cgggggcgcc gccggcctcg
      781 cggcgcagcg gcgcctcggc gagccatgcc gcgacggcct cgcgcgccag cggtcgcggg
      841 ccgccggacg ccagcgccag cattcccagc agcgcccgcc cggtcgggct gtcccccagc
      901 cgggtcctgg gaccgccgtt gctcggcagg cccgccgccg ccagggcgtc gcgcaccagc
      961 agcgcgtacg ggcggtcagc ggtccacagc agggccatgc ggtgcagcgg cgcgccggcg
     1021 tcgaagcgct cggcgatgcg ccgcaccgcg gcgcgcacct ccgtctccgg gtccggcgcc
     1081 gacaggacgc gcaccgtgcc gtccggcgcg gcgtcggcaa cggcgggttg gggcgctcgg
     1141 cccagctcgc agcccagggc gtcggcggct tgcagcgcac ccgcgtcggc gcgctcgtcg
     1201 ccggtcaggg ccaccaggac cgtgacgggg ccctggccgg ccaccgcgcg caggaacgcc
     1261 gtggcggccg gggacagggc gcgatccacg atggccacca cggcgcccag ctcggcggcg
     1321 gccgcgggtc gctcggcggc cagcgaggcg gcggtgcgca gcaggtcgtg ctcgtcgtag
     1381 cggtcgtgca gcagggcgcg caccgcgccg gcgacacggg ccgcctcgcg gccacgcgcc
     1441 ctggacaggc cggcgagcgc gccggccggg acgctgccca ggtcgcgcac ggtgcgcgcg
     1501 agcgcccgcg ccgtggcggg atggtcggcc acgggcgcga accggccggg cgcctggtgc
     1561 agcacggcgc gcacggcctc ggtcagcacg gcggggcgca gggggcgctt gcccgctgcg
     1621 gcgagttccg gcgcggccag cagttcggcc gcgcgggccg ccagctcgaa gcgcacggcg
     1681 gccatgccgc cgaggcggcc ggccacctgt cgccgcagcg ccacgccggc cgaacgcgtg
     1741 ggcaccagca ccgtcacgcc gcgcagcggg tcaccgtcct ggaggccggc caccgtgtgc
     1801 gcgaccgcct cggccagcgc ggggccgtgg ccgacggccc gcgcctgcaa ggccggcggg
     1861 ctgagcgtct ggggcggatc ggtgcgggag ctcacgtggg cagcagggta ccggcgggct
     1921 gcgacgcccg gacgggcggc gcgccgatcg ggcaggacgc catggccgct ttgccagcgc
     1981 ggccaccggg tgtccacact gtgacgcacg ccgcgtcgtc cccagcgcgc ggatgcgccc
     2041 ggagggcgcc acaacccaag gagcgtcggt gacgttggac ctgcctggcg tcaagcggct
     2101 gatcccgctg cccgaggacg gtgccgagct gccaaaccac accccgcgct gcatggtgtg
     2161 cggcagggac aaccccgagg gctggcacct caccgtgcgg cgccacggcg acgaggtgcg
     2221 ctgcgagttc accttcgacc gccggcacga gggcggcccc gggctggcgc acggcggcgc
     2281 cgtcgcggcc gtctgcgacg acgtgctcgg ccacgtcctc accctgctgg gcgtgccggc
     2341 ggtcacgcgc cgcctggagg tcgactacct caagccggtg ctgctcggtg agccgcacac
     2401 gatggcggga cgcatcgagt cggtggacgg ccgcaaggtc tggctcgcga tggaggcgct
     2461 cgcgccggac gggcagccgc gcttcacggc gcgcgggctg ttcatccgcg tcggcctcga
     2521 gcacttcctc gccgggctgt cgccggagga gcgggaacgg gcgcaggaga ggctcgcgga
     2581 gctgcgcgag cgtggagagg acgtgaccgc atggtgagcg acgtgggcgg gatccggg
//
LOCUS       DATMKC010000042         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_113642, whole genome shotgun sequence.
ACCESSION   DATMKC010000042 DATMKC010000000
VERSION     DATMKC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113642"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VNU01_01130"
     CDS             complement(<1..1064)
                     /locus_tag="VNU01_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWH31252.1"
                     /translation="MSAGDSGNTTIVVDTSAVTATRRRANTELGLLVLAVVIVSTAYA
                     LVGLAQEPVLPAGLVPYGGALLALALAAHLVRRRLAPHADPLLLPLAFLLNGLGLVMV
                     RRIDFAYAVRDNPPSALAPAQTLWSIVAVAAFCATLALLRDHTVLDRYRYSIGLAALL
                     LLVLPLLPVVGREINGARLWLRIAGFTIQPAEVAKLGLVVFFASYLAEKRQLLSVATS
                     RLGPFMVPPPRAFAPVAAIWLASLVVLVTQNDFGLSLLFFGIFVVLLYAATARAAYVA
                     VGTGLFLFGATAAYLLFGHVRLRVDVWLDPWSQYAGGGYQLAQSLFALGTGGIFGVGW
                     GQGRPDFIPFVQTDFIFAAFG"
     gene            complement(1061..2473)
                     /locus_tag="VNU01_01135"
     CDS             complement(1061..2473)
                     /locus_tag="VNU01_01135"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWH31253.1"
                     /translation="MRISAHAATDVGRVREGNEDSLFAGRAVFAVADGMGGHQAGEVA
                     SEAALRPIAALDGQEFAAPEAAQDALVEAVVDANRSVVSQARSDPSLRGMGTTLTAVL
                     VRDNHLHIAHVGDSRAYLLRGGRDMTQLTTDHTLVEQLIREGRLARSEAAVHPQRSVI
                     TRAIGVDQQVQVDSLPPLQLQPGDQVLLCSDGLTGPVDDHTIAEVLAGTADGDTACRQ
                     LIEAANAAGGPDNITVVLLRLEGDPAAAARTPLPGTKAATLIPDPTEEIASMGGTDAA
                     RRELRPGDVEAVTIRTRDDEAHDFTDAMARYGDRREVQRGALGRSRGVPRWAVMLGAA
                     AILAGVLGGGAFLLLQRAYWLGIDEGRVTVFHGMPQDVAGIPLHWVAERSDLRAARLP
                     DRLQDRLREGVALGSLEEGLELVETYEDDLEAEERRRRAREAVISPSPSPSPSPTASP
                     SPATSPAPGAVTSAGAQPAP"
     gene            complement(2497..2973)
                     /locus_tag="VNU01_01140"
     CDS             complement(2497..2973)
                     /locus_tag="VNU01_01140"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWH31254.1"
                     /translation="MPPVVLTLLQALFLLLLYVFVGRAVRTIARDLSPAGATAVRRRS
                     VRPTAPRRRQESRKEPRELVVHLPGVAPRVIPLADQQDGTGVTFGRAPDMTVPLDDPY
                     ASDRHARVYRTGEGWVLADLGSTNGTYRNKEKVSAPVRLVAGDQFAIGKTVVEVRR"
     gene            complement(2976..>3525)
                     /locus_tag="VNU01_01145"
     CDS             complement(2976..>3525)
                     /locus_tag="VNU01_01145"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWH31255.1"
                     /translation="SEVVRRTATERNWHLQGPVRIEFERNSGVPVGTYELRGKVEAPV
                     SAMAPPREPAPEADRGFPAPAPAPAPSPAAQGEASVLAPSQSRRATLHLTAGPEAGRR
                     FAIAGGAILGRLPECDVSLDDPSVSRRHARVGRQDGVWFVEDLGSTNGTRVGERAVTR
                     TELADGDELRLGNVRLVFRLET"
BASE COUNT          450 a         1284 c         1354 g          437 t
ORIGIN      
        1 ccgaacgcgg cgaagatgaa gtcggtctgc acgaacggga tgaagtccgg ccgcccctgg
       61 ccccagccca ccccgaagat gccgccggtg cccagcgcga acagcgactg cgcgagctgg
      121 tagccgcccc cggcgtactg gctccacggg tccagccaca cgtcgacgcg cagccgcacg
      181 tggccgaaca gcaggtaggc cgccgtcgcg ccgaacagga acagcccggt gcccacggcc
      241 acgtaggcgg cgcgcgccgt cgcggcgtac agcagcacca cgaagatccc gaagaacagc
      301 aggctgaggc cgaagtcgtt ctgcgtgacc aggacgacca ggctcgccag ccagatcgcc
      361 gccacgggcg cgaaggcgcg cgggggcggc accatgaacg ggcccaggcg gctggtcgcc
      421 acggacagca gctggcgctt ctccgccagg tagctggcga agaacaccac caggcccagc
      481 ttggcgactt cggcgggctg gatggtgaag ccggcgatgc gcagccacag ccgggcgccg
      541 ttgatctcgc ggccgaccac cggcagcagc ggcagcacca gcagcagcag cgccgccagg
      601 ccgatcgagt agcggtagcg gtcgaggacc gtgtgatcgc gcagcagcgc cagcgtggcg
      661 cagaacgccg ccacggccac gatgctccac agcgtctggg ccggcgcgag cgccgacggg
      721 gggttgtcgc gcaccgcgta ggcgaagtcg atgcggcgga ccatgaccag gcccaggccg
      781 ttgagcagga acgccagggg cagcagcagc gggtcggcgt gcggggccag gcgccggcgc
      841 accaggtgcg ccgccagcgc cagggccagc agcgcgccgc cgtagggcac caggccggcc
      901 ggcagcaccg gctcctgcgc caggcccacc agtgcgtagg cggtggagac gatcaccacg
      961 gccaggacca gcagtcccag ctcggtgttc gcgcgccggc gcgtcgccgt gaccgccgac
     1021 gtgtcgacga cgatggtggt gttgccgctg tcgcccgcgc tcatggggcc ggctgcgctc
     1081 ccgcggaggt gacggcgccg ggcgcgggcg aggtggctgg gctcgggctc gcggtggggc
     1141 tcgggctcgg gctcgggctc gggctgatga ccgcctcgcg tgcccgccgt cgccgttcct
     1201 cggcctcgag gtcgtcctcg taggtctcga ctagctccag gccctcttcc aggctgccca
     1261 gggcgacgcc ctcccgcagg cggtcctgga ggcggtcggg caggcgcgcg gcccgcaggt
     1321 cgctgcgctc ggcgacccag tgcaagggga tgccggcgac gtcctgcggc atgccgtgga
     1381 acacggtgac gcgcccttcg tcgatgccga gccagtaggc ccgctgcagg agcaagaacg
     1441 cgccgccgcc cagcacgccg gccaggatcg cggcggcgcc cagcatgacg gcccagcgcg
     1501 gcactccccg cgagcgcccc aaggcccccc gctgcacttc gcggcggtcg ccgtagcgcg
     1561 ccatcgcgtc ggtgaagtcg tgcgcctcgt cgtcgcgcgt gcgaatggtc accgcctcga
     1621 cgtcgcccgg ccgcagttcc cggcgggcgg cgtccgtccc gcccatcgag gcgatctcct
     1681 cggtggggtc ggggatcagc gtggcggcct tggtgcccgg cagcggcgtg cgggccgccg
     1741 cggcggggtc gccctccagg cgcaacagga ccaccgtgat gttgtcgggc ccgcccgccg
     1801 cgttggccgc ttcgatgagc tggcggcagg ccgtgtcacc gtcggccgtg ccggccagca
     1861 cctcggcgat ggtgtggtcg tcgaccggcc cggtcaggcc gtccgagcac agcagcacct
     1921 ggtcgccggg ctgcagttgc agcggcggca gggagtcgac ctgcacctgc tggtccacgc
     1981 cgatcgcgcg ggtgatgacg ctgcgctgcg ggtgcaccgc cgcctcgctg cgcgccaggc
     2041 ggccctcgcg gatgagctgc tcgaccaggg tgtggtcggt ggtgagctgg gtcatgtcgc
     2101 ggccgccgcg caacaggtag gcgcggctgt cgccgacgtg ggcgatgtgc aggtggttgt
     2161 cgcgcaccag cacggcggtc agcgtggtgc ccatgccgcg cagcgacggg tcggagcggg
     2221 cctggctgac cacgctgcgg ttggcgtcga cgacggcctc gaccagcgcg tcctgggcgg
     2281 cctccggggc ggcgaactcc tggccgtcca gtgcggcgat gggacgcaaa gcggcctcgc
     2341 tggccacttc gccggcctgg tgcccgccca tgccgtcggc gacggcgaag acggcccgtc
     2401 ccgcgaacag gctgtcctcg ttgccttcgc ggacgcggcc gacgtcggtc gccgcgtgcg
     2461 cgctgatccg catgtgcgtg tctcctgtcg cggcggctag cgccgtacct cgaccaccgt
     2521 cttgccgatg gcgaactggt cgcccgccac cagccgcacc ggggcgctga ccttctcctt
     2581 gttgcggtag gtgccgttgg tggagccgag gtcggccagc acccagcctt cccccgtccg
     2641 gtacacgcgt gcatggcggt cgctggcgta cgggtcgtcc agcggcaccg tcatgtcggg
     2701 cgcccggccg aacgtcaccc cggtgccatc ctgctggtcg gccagcggga tgacgcgcgg
     2761 agccacgccg ggcaggtgta ccacgagctc gcgcggctcc ttgcggcttt cctgccgccg
     2821 gcgcggcgcg gtgggccgca cgctccgccg gcgcacggcg gtcgcgcccg ccggggacag
     2881 gtcgcgggcg atggtgcgca cggcccggcc cacgaagacg tacagcagca gcaggaacag
     2941 cgcctgcagc aaggtcagga cgaccggggg cacggctacg tctccaggcg gaacaccagc
     3001 cggacgttgc ccaggcgcag ctcgtcgccg tcggcgagct cggtgcgggt gaccgcccgc
     3061 tcgccgacgc gggtgccgtt ggtggagccc aggtcctcca cgaaccagac cccgtcctgg
     3121 cggccgacgc gggcgtggcg ccgcgacacc gacgggtcgt ccaggctgac gtcgcattcc
     3181 ggcagccggc ccaggatggc gccgccggcg atcgcgaagc ggcggcccgc ctccgggccg
     3241 gcggtcaggt ggagggtggc gcggcggctc tggctgggcg ccagcacgga ggcctcgccc
     3301 tgggcggccg gcgagggcgc cggtgccggt gccggtgcgg ggaagccgcg gtcggcttcg
     3361 ggggccggct cgcgtggggg cgccatggcg ctcaccgggg cctccacctt gccgcgcagc
     3421 tcgtaggtgc cgacggggac gccggagttg cgctcgaact cgatgcgcac cggcccctgg
     3481 aggtgccagt tgcgctccgt ggcggtgcgg cgcacgacct cggac
//
LOCUS       DATMKC010000043         5352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_165779, whole genome shotgun sequence.
ACCESSION   DATMKC010000043 DATMKC010000000
VERSION     DATMKC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165779"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..273
                     /locus_tag="VNU01_01150"
     CDS             94..273
                     /locus_tag="VNU01_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31256.1"
                     /translation="MGKRHSADTGNAAVEKAFRDRGYKGASYVVRDANAAHRPQGWEI
                     KEAAPARASSKRRAG"
     gene            complement(276..1019)
                     /locus_tag="VNU01_01155"
     CDS             complement(276..1019)
                     /locus_tag="VNU01_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWH31257.1"
                     /translation="MRSDVTLNLAAEPHVVVGPNRRADFELCPRRYRNKQLLHLAGLD
                     EMEGAGTVRGQAVHEELHHRHEIASLHDEDEPQLAGARDLRDPLVARALATHRRVCPG
                     ARGARYLGGELTLLWAPPRERLLVHGRIDALWEWPGGVLEIRDYKTGATGDDLSLDFS
                     ARVYALLVAADPRWRGGRRIIRVTFERLLDDPVEVSIDADAPFLRDAVEAVRSFARRL
                     REESEWPAKPSEFCGRCRYRESCPSSAAG"
     gene            complement(1009..1776)
                     /locus_tag="VNU01_01160"
     CDS             complement(1009..1776)
                     /locus_tag="VNU01_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31258.1"
                     /translation="MDPEPLRVRPSLLPVAAQCRLRFQREVRGGVGMVRFSRANADRA
                     FLVRNLLVDAIEQAHAAGPDGLDQLLHADPPEGLLAEERWLFRRGLDHYVELAGERGG
                     AVLQPDERYPVLPHSSGRFLLSLRLELCFTVEDGGAELRRIGFAAPRQDPGEDPRVLA
                     VAVLLAHRGHTFVRVAHLDLLHGRMQESLLDRPRIAEAARDIGEIVLSALDDPRPEPA
                     PGWWCHECPALRSCPAVSQQAPSELVLMATGEAMGEV"
     gene            1841..2521
                     /locus_tag="VNU01_01165"
     CDS             1841..2521
                     /locus_tag="VNU01_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HWH31259.1"
                     /translation="MDVTKARARAALDTGVEPVGTYRFGDSAALSDELLDLVLRGPKR
                     ATAFAEADVEQGGAPYPEPGQHWLLLDGAGQAHCVARVDQVAGGPLYTVDAAFAWETG
                     WRNRTRAAWIAAHEAYLRRQGFTDLESERVLFIRFSVVWPEADEPRWWAPGVRELRGH
                     EPAPDSPRPDLPALAAFDGERVTGVLRFEPEPGDLRLVAVVADDAGVESRLREALEQR
                     RRADRAVR"
     gene            complement(2518..3015)
                     /locus_tag="VNU01_01170"
     CDS             complement(2518..3015)
                     /locus_tag="VNU01_01170"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HWH31260.1"
                     /translation="MPLLVLLFIVVPLGEVWLIIRVAGLAGWGNTVALLFVVSVVGAV
                     MVKREGAKAWRAFARALQEARVPAREVVDGALVLVGGALMVTPGFFTDVVGLVCVLPP
                     TRALVNRALRSQVRGMFGLGALDRWGRTRGPGARPGDRDEPATLDVEVVDVRRDDPPP
                     GQGSG"
     gene            complement(3015..3881)
                     /locus_tag="VNU01_01175"
     CDS             complement(3015..3881)
                     /locus_tag="VNU01_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWH31261.1"
                     /translation="MPPRSLWKGALSFGLVNVPVRLYTATDDKTPSFNQLRASDHSRI
                     GYKRVAKADDQEVDYDDIVKGYEYERDRYVVFTPEELEAMKPPSSRTIEILQFTPLEQ
                     IDPIYFQRAYYLAPDPSGAKAYALLAQAMKDRKTVALCKVAIRDKEHLATLRLRDDVM
                     VLETMWWPDEIREVSLADFDIDELPEPRKQEVQMAQTLIENLTEDFDPSQFSDTYRER
                     VMEAVKRKVEGEEITIADEGEEPAAVVDLMAALKQSVEASKSKRGGDKQPKAAEKDDK
                     DEDKPKKRKKAS"
     gene            complement(3973..4275)
                     /locus_tag="VNU01_01180"
     CDS             complement(3973..4275)
                     /locus_tag="VNU01_01180"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWH31262.1"
                     /translation="MSWAAILALAAGTFLLKGVGPLLAGGRTMPPGLDRLVRLLPPAL
                     LAALVAVQTFGDGRELVLDGRAVGVAAGAVAVLLRAPFIVVVLVAMAAAAGWRALT"
     gene            complement(4272..4913)
                     /locus_tag="VNU01_01185"
     CDS             complement(4272..4913)
                     /locus_tag="VNU01_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWH31263.1"
                     /translation="MRKALVIGVAVGIYGVSFGVLAVAAGVSVWQAVGLSALVFAGGS
                     QFAAVGVIAAGGAPLTAVAGGLLLNSRMAAFGLALAPVLGGSVPTRMLAAHLLIDESS
                     AMALAEDDPRAARRAFWLTGVSVFVCWNAATLFGALAGSLIGDPEAIGLDAAFPAGFL
                     ALLGPLLTDRAARASALAGALIALALLPFTPAGVPILAASAGVLGGLLARRAA"
     gene            5032..5349
                     /locus_tag="VNU01_01190"
     CDS             5032..5349
                     /locus_tag="VNU01_01190"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31264.1"
                     /translation="MATAPTTIHFPRQLQTRKRGTHWYAQMDGTELKLSNLDKVFWEA
                     EGHLKGDLVSYYLNIAPTLLPYLRDRPLTLKRMPDGADGDFFYEKQAPAYTPEWLRRV
                     PVT"
BASE COUNT          764 a         2044 c         1905 g          639 t
ORIGIN      
        1 gtctaggccg cccccttcgt ccccggcacc ccgccttcgc gtcacagctc acgcgtacgc
       61 tacggcgacc tcgacctgct ggaaggaggg acgatgggca agcggcacag cgccgacacc
      121 gggaacgccg cggtggagaa ggccttccgt gatcgtgggt acaagggcgc gagctacgtg
      181 gtgcgcgacg ccaacgccgc ccatcgcccg cagggctggg agatcaaaga agcggcgccg
      241 gcgcgtgcct cgtcgaagcg gcgggcgggc tgacttcacc cggccgctga gctggggcag
      301 gactcgcggt agcggcagcg gccgcagaac tcgctcggct tggcgggcca ctccgactcc
      361 tcgcgcagcc gccgcgcgaa ggagcgcacc gcctccacgg cgtcgcgcag gaagggcgcg
      421 tcggcatcga tggacacctc gacgggatcg tccaggaggc gctcgaacgt gacgcggatg
      481 atgcggcgcc ccccgcgcca gcgcgggtcg gcggcgacca gcagcgcgta cacccgcgcg
      541 gagaagtcca ggctgaggtc gtcgcccgtc gcaccggtct tgtagtcgcg gatctccagc
      601 accccgccgg gccactccca cagcgcgtcg atgcgcccgt gcaccagcag ccgctcgcgc
      661 ggcggcgccc acagcagggt gagctcgccg cccaggtagc gggcgccccg cgccccgggg
      721 cagacccggc gatgcgtggc cagcgccctg gcgacgagcg ggtcgcgcag gtcgcgcgcc
      781 ccggccagct gcggctcgtc ctcgtcgtgc agggaggcga tctcgtggcg gtggtgcagc
      841 tcctcgtgca ccgcctgccc gcgcaccgtc ccggcgcctt ccatctcgtc caggcccgcc
      901 agatgcagca gttgcttgtt gcggtagcgg cgcgggcaca gctcgaagtc ggcgcggcgg
      961 ttggggccca ccaccacgtg cggctccgcg gccaggttca gcgtcacgtc agacctcacc
     1021 catggcctcg ccggtggcca tcagtaccag ctcggacggc gcctgctggc tcaccgcggg
     1081 gcacgagcgc agcgccgggc actcgtggca ccaccagccg ggcgcgggct cgggtcgcgg
     1141 gtcgtccagc gcggacagca cgatctcgcc gatgtcccgc gccgcctcgg cgatgcgcgg
     1201 ccggtccagc agggactcct gcatgcgccc gtgcagcagg tccaggtggg ccacgcgcac
     1261 gaacgtgtgg ccgcggtggg ccagcagcac cgccacggcc agcacgcgcg ggtcctcgcc
     1321 ggggtcctgg cgcggcgcgg cgaagccgat gcggcgcagc tccgcgccgc cgtcctccac
     1381 ggtgaagcac agctccaggc gcagcgacag caggaagcgg ccgctggagt gcggcagcac
     1441 ggggtagcgc tcgtccggct ggagcaccgc gccgccccgc tcccccgcca gctccacgta
     1501 atggtccagc ccgcggcgga acagccagcg ctcctccgcc agcagtccct cgggcgggtc
     1561 ggcgtgcagc agctggtcca ggccgtccgg cccggcggcg tgcgcctgct cgatcgcgtc
     1621 gacgagcagg ttgcgcacca ggaaggcccg gtcggcgttg gcccgggaga agcgcaccat
     1681 gcccaccccg ccgcgcacct cgcgctggaa gcgcaggcgg cactgcgccg ccacgggcag
     1741 cagcgacggc cggacgcgca ggggctcggg gtccatgcgc agcacggtag cggacgcctg
     1801 tgacgcacga ctccgccacg tttcgcatac gtttacgcgt atggatgtga cgaaagcccg
     1861 cgcgcgggcg gcactggaca ccggcgtcga gcccgtgggc acgtaccggt tcggcgactc
     1921 cgccgccctt tccgacgagc tgctggacct ggtcctgcgc gggcccaagc gcgcgaccgc
     1981 cttcgcggag gccgacgtcg agcagggcgg cgcgccctac cccgagcccg gccagcactg
     2041 gctgctgctg gacggcgccg ggcaggccca ctgcgtcgcg cgcgtggacc aggtcgccgg
     2101 cggccccctg tacacggtgg acgcggcgtt cgcctgggag accgggtggc gcaaccgcac
     2161 gcgcgccgcc tggatcgccg cgcacgaggc gtacctgcgg cgccagggct tcacggacct
     2221 ggagtcggag cgggtgctgt tcatccgttt cagcgtcgtg tggcccgagg ccgacgagcc
     2281 gcgctggtgg gcgccgggcg tgcgggaact gcgcggccac gagccggcgc cggactcacc
     2341 gcgccccgac ctgccggcgc tggcggcctt cgacggcgag cgcgtcaccg gggtgctgcg
     2401 cttcgagccg gagcccggcg acctgcgcct ggtcgcggtg gtggcggacg acgccggtgt
     2461 ggagtcgcgg ctgcgcgagg cgctcgagca gcgccggcgc gcggaccgcg ccgtccgcta
     2521 gccggagccc tggcccggcg gcgggtcgtc gcggcgcacg tcgaccacct ccacgtccag
     2581 cgtcgcgggc tcgtcgcggt cgcccggccg cgcgccgggc ccgcgggtgc ggccccaccg
     2641 gtccagcgcg cccagaccga acatgccgcg aacctggctg cgcagcgccc ggttgaccag
     2701 ggcgcgcgtc ggcggcagca cgcacaccag ccccaccacg tcggtgaaga acccgggcgt
     2761 gaccatcagc gcgccgccca ccagcaccag ggcgccgtcg accacctccc gcgccggcac
     2821 gcgcgcctcc tgcagcgcgc gcgcgaacgc ccgccacgcc ttggcgccct cgcgcttgac
     2881 catcaccgcg cccaccacgg acaccacgaa cagcagcgcg accgtgttgc cccagccggc
     2941 caggccggcg acgcggatga tcagccacac ctcgccgagg gggacgacga tgaacagcag
     3001 gaccagcagg ggcactacga cgccttcttg cgcttcttgg gcttgtcctc gtccttgtcg
     3061 tccttctccg cggccttggg ctgcttgtcg ccgccgcgct tggacttgga ggcctcgacc
     3121 gactgcttga gcgccgccat gaggtcgacc accgcggcgg gctcctcgcc ctcgtcggcg
     3181 atggtgatct cctcaccctc gaccttgcgc ttgaccgcct ccatgacgcg ctcgcgatag
     3241 gtgtcggaga actggctggg atcgaagtcc tcggtgaggt tctcgatgag cgtctgcgcc
     3301 atctgcactt cctgcttgcg cggctccggc agctcatcga tgtcgaagtc ggccagggag
     3361 acctcgcgga tctcgtccgg ccaccacatc gtctccagca ccatgacgtc gtcgcgcagg
     3421 cgcagcgtgg ccaggtgctc cttgtcgcgg atggcgacct tgcacagcgc gacggtcttg
     3481 cggtccttca tcgcctgcgc cagcagggcg tacgccttcg ctcccgaggg gtccggcgcc
     3541 aggtagtagg cgcgctggaa gtagatcgga tcgatctgct ccagcggggt gaactgcagg
     3601 atctcgatgg tgcggctgga cggcggcttc atcgcctcca gctcctccgg cgtgaacacc
     3661 acgtagcggt cgcgctcgta ctcgtagccc ttgacgatgt cgtcgtagtc gacctcttgg
     3721 tcgtcggcct tggcgacgcg cttgtagccg atgcggctgt ggtcggaggc gcgcagctgg
     3781 ttgaacgacg gcgtcttgtc gtcggtcgcg gtgtacaggc gcaccggcac gttgaccagg
     3841 ccgaaggaca gtgcgccctt ccacagactg cgcggcggca ttgcggacct cctcgatgcg
     3901 gggaagggaa tcagtgtcct acccgagagc ccgcaccgcc acccattccg taaaggcccg
     3961 cccgcgacgc ggtcaggtca gggcgcgcca gccggccgcg gcggccatcg ccaccagcac
     4021 caccacgatg aacggcgccc gcagcagcac cgccaccgcc cccgcggcca cgccgaccgc
     4081 gcgcccgtcg agcaccagtt cccgcccgtc gccgaacgtc tgcaccgcca cgagggccgc
     4141 cagcagcgcc ggcggcagca ggcgcaccag ccggtccagc ccgggcggca tcgtgcgccc
     4201 gccggccagc agcggcccca cgcccttgag caggaacgtg ccggccgcca gcgccaggat
     4261 cgccgcccag ctcacgcggc cctcctggcg agcaggccgc cgagcacccc cgccgaggcg
     4321 gccaggatcg gcacgccggc cggggtgaac ggcagcagcg cgagcgcgat cagcgccccc
     4381 gccagcgccg aggcgcgcgc cgcgcggtcg gtgagcagcg ggcccagcag cgccaggaac
     4441 ccggcgggga acgcggcgtc cagcccgatc gcctccgggt cgccgatcag cgagccggcc
     4501 agggcaccga acagcgtggc cgcgttccag cacacgaaga cgctcacccc cgtcagccag
     4561 aacgcgcggc gcgccgcgcg ggggtcgtcc tcggccagcg ccatcgccga ggactcgtcg
     4621 atcagcaggt gcgcggcgag catgcgcgtg ggcaccgagc cgcccagcac gggcgccagc
     4681 gccaggccga acgccgccat gcgcgagttg agcagcaggc cgccggccac cgcggtcagc
     4741 ggcgcgcccc ccgccgcgat gacacccacg gcggcgaact gggagccgcc cgcgaacacc
     4801 agcgcggaca ggcccacggc ctgccacacc gagacgccgg ccgcgaccgc cagcacgccg
     4861 aagctcaccc cgtagatgcc caccgccacg ccgatgacga gcgccttgcg cacactgggg
     4921 tcgcggaggc cacggccagc cggtggcggg ctgggcggcg gcgcggcgga gggggacgaa
     4981 gcggacatcg ctggtaggaa accccatgcg tagggcaggc tgcgagccga aatggcaacc
     5041 gcgcccacaa ccatccactt cccgcgccaa ttgcagacgc gcaaacgcgg cacgcactgg
     5101 tacgcccaga tggacggcac ggagctcaag ctgtccaacc tggacaaggt cttctgggag
     5161 gcggaaggcc acctcaaggg cgacctggtc tcctactacc tcaacatcgc gccgacgctg
     5221 ctgccctacc tgcgggaccg gccgctgacg ctcaagcgca tgccggacgg cgcggacggc
     5281 gacttcttct acgagaagca ggcgcccgcc tacacgcccg agtggctgcg gcgcgtcccg
     5341 gtaacgtagc gg
//
LOCUS       DATMKC010000044         5706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_334546, whole genome shotgun sequence.
ACCESSION   DATMKC010000044 DATMKC010000000
VERSION     DATMKC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334546"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1818)
                     /locus_tag="VNU01_01195"
     CDS             complement(130..1818)
                     /locus_tag="VNU01_01195"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HWH31265.1"
                     /translation="MTSVTERGTEAPAFLPGLMQPTPLVLHAVFERMRTIYGDTRVAG
                     PEGVFTYAELGERILRLCRVLVEELDVRPGDRVATFGFNSVRHFELYWAVPLVGAVLH
                     TVNVRLFPEQIAQIVGHAGDKVLFFDGEVADRLGDVDGLGIVEQWVRMQPGGDAALPT
                     LTPEVREYEDLLAACPDPIGALPDVPEDAAANLCYTSGTTGMPKGVLQSHRSLWLHAM
                     AACMADHLALTQHDTVLPIVPLFHACGWGMPYAAPFTGAGLVFHGSDSFPANVARLIR
                     EHRVTVGAAVPTIWKELLPLLRSGDADLSTVRGIYVGGSATPRPLAEAYGAHGIDMVQ
                     IWGMTETGPLACVSQPRRHHGRDVRGDALYAIKEKTGTIMSGLEARVVGETGEAVPWD
                     GETVGELEVRGPWIAAAYYNDPIGSVEKLHDGWLRTGDMAWMERDGTFRIVDRAKDLV
                     KSGGEWISSVELEGALLGHPAIRDAAVVGLPHPKWDERPVAFVVADGHGPGQEPTVES
                     VHAFLADKVAKWWLPDAVVVVEEIPKTSVGKIDKKVLRAAWEARAGELFGIREG"
     gene            complement(1815..3050)
                     /locus_tag="VNU01_01200"
     CDS             complement(1815..3050)
                     /locus_tag="VNU01_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH31266.1"
                     /translation="MTPDATRGQDVGGLFRHDPEAARQVARRVLATIDELLPRMTTAY
                     REEIPEYGALDAGVVEAEVAPVSREVVEEFFSAILTEGEPDPTVPVDFRAAGRRRLEI
                     GVPLDSALHAFRIAGRVVWNAVVEATQPGEEHVLARLAGDWIDYVDRISSAFAEAYLS
                     ASHEQLRRADARRRAVLDAVLAAGDAAELAAVASRWSVTLARAYVPMLVEDDHSSGLI
                     DRLLDAAPPHTLAGTRGHRLLALVPAPGADIPALARAVGARLAARGEAAPPGPVLATE
                     VARAETLLEAAAAAGRHGGVLAPDDLLLEQLVTDSPRLAEVLRHKVMDVFRERGDEHL
                     LGTLRRYLACGSVPETAREDCVHVNTVAYRLKRVRELTGLDPRVPAEAAQLVLGLTAM
                     DVAEFAERADGEAGHGGDR"
     gene            complement(3047..3853)
                     /locus_tag="VNU01_01205"
     CDS             complement(3047..3853)
                     /locus_tag="VNU01_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH31267.1"
                     /translation="MSAVSAHTSVRPPSQPPPLLRVSGLRAGFGANAVLHGVGFDVEP
                     GSITAVLGLNGAGKSVTLKVIAGLVPAWSGQVRLDGEDVTPLSPERRVGAGMGHVPQG
                     RQVFPQLTVEENLRLGAYLLRRADRARYDRVVAGLYERFPRLAERRRQLGGTLSGGEQ
                     AMLAVARALVNEPKLLLVDEPTAGLAPAIVEELAELLTDVNRDGLTILLVEQHIAFAL
                     AVADRAEVLQHGRVVASAPVDELDRDNLASLLGIGRLMSASLDPDHGVGR"
     gene            complement(3850..>5706)
                     /locus_tag="VNU01_01210"
     CDS             complement(3850..>5706)
                     /locus_tag="VNU01_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HWH31268.1"
                     /translation="LPRGKAGALLVLAAFAILPPLLGRLGVLTDFRAVMLGFGACYAA
                     MALSLNVLMGYAGQISLGHAALIGAGAYASGILTGRAGLPMLAGVVVAALVGAAAAFL
                     VGLPALRLRGLYLAITTIGFAVAMEQAVFRLPWVSGGSAGVSLPRPAAGAFVFTRNPD
                     YLALVLVFVLGFWLLDGNITRSKLGRAFQGLRENEQVAQAYGVDVSRYKLLAFVISGA
                     LAGVSGALYGHLLLFVESRNFSFLFSLTLVTFVVVGGLGSRVGVVVAAALFGIVPRML
                     TVFEGWDLVIGSALLVFTMARHPTGFAGALREAREKKAAEHARAVQGGGPAAEGAVPA
                     FTHAAGARLAAADAGSAGREVVLAVRDVRVRFGGLVAVDGAGLEVPKGRIVGLIGPNG
                     AGKTTLFNAISGFVAAESGSVSLRGRELAGLPPHERALLGLGRTFQTAGLARGLSLLD
                     NVMLAQHRLLGYATAPALLRTGGVARAERDLEARSREVVAALGMTAHADLPLKHLSGG
                     QQRIAELACALVTDPEVLLLDEPSAGMAPAAVEQLAERLRELRDDLGRTVLLIEHHIP
                     LVMDVCDEVVVLNLGQVLAAGGPDEVVRDQAVLDAYLGAGFAAKTKAAEVAP"
BASE COUNT          781 a         2198 c         2065 g          662 t
ORIGIN      
        1 tgaggtggtt ggcggtgatg ccgtgcgccg ccaggaccac cggcgcctgc cccggctcgg
       61 cgccccatcg ggcggtcagc aggctgccgc cggcgaccgg cacgtcgtgg aatcggggtg
      121 cgctcacgtt catccctccc ggatgccgaa cagctcgccc gcgcgcgcct cccaggcggc
      181 gcgcagcacc ttcttgtcga tcttgccgac gctggtcttg gggatctcct cgaccaccac
      241 cacggcgtcg ggcagccacc acttcgcgac cttgtcggcc aggaacgcgt gcaccgactc
      301 gaccgtgggc tcctggcccg ggccgtggcc gtcggccacg acgaacgcca ccggccgctc
      361 gtcccacttc gggtgcggca gccccaccac ggcggcgtcg cggatcgcgg ggtgccccag
      421 cagcgcgccc tccagctcca ccgagctgat ccactcgccg ccggacttca ccaggtcctt
      481 ggcgcggtcc acgatgcgga acgtgccgtc gcgctccatc cacgccatgt cgccagtgcg
      541 cagccacccg tcgtggagct tctccaccga gccgatcggg tcgttgtagt aggcggcggc
      601 gatccacggc ccgcgcacct ccagctcacc gaccgtctcg ccgtcccacg gcaccgcctc
      661 gccggtctcg ccgacgacgc gcgcctccaa gccggacatg atcgtgccgg tcttctcctt
      721 gatcgcgtac agcgcgtcgc cccgcacgtc gcggccgtga tgccgccgcg gctgcgacac
      781 gcacgccagc ggccccgtct cggtcatgcc ccagatctgc accatgtcga tgccgtgggc
      841 gccgtaggcc tccgccagcg gccgcggcgt ggccgagccg cccacgtaga tgccgcgcac
      901 cgtggacagg tccgcgtcgc cggagcgcag cagcggcagc agctccttcc agatcgtcgg
      961 caccgcggcg cccacggtga cgcggtgctc gcggatgagc cgcgccacgt tcgccggaaa
     1021 gctgtccgag ccgtggaaca ccaggcccgc gccggtgaac ggggcggcgt agggcatgcc
     1081 ccagccgcag gcgtggaaca gcggcacgat cggcagcacg gtgtcgtgct gggtgagcgc
     1141 caggtggtcg gccatgcagg cggccatggc gtgcagccac agcgagcggt gcgactgcag
     1201 cacgcccttg ggcatcccgg tcgttcccga cgtgtagcag aggttcgcgg ccgcgtcctc
     1261 cggcacgtcc ggcagcgcgc cgatcgggtc ggggcacgcg gccagcaggt cctcgtactc
     1321 ccgcacctcc ggcgtcagcg tgggcagcgc ggcgtcgccg ccgggctgca tgcgcaccca
     1381 ctgctccacg atccccaagc cgtccacgtc gccgaggcgg tcggcgacct cgccgtcgaa
     1441 gaacagcacc ttgtcgccgg cgtggccgac gatctgggcg atctgttcgg ggaacagccg
     1501 cacgttcacg gtgtgcagca cggcgcccac cagcggcacc gcccagtaca gctcgaagtg
     1561 gcgcaccgag ttgaagccga acgtcgcgac gcggtcgccc gggcgcacgt ccagctcctc
     1621 caccaggacg cggcacagcc gcaggatgcg ctcgcccagc tcggcgtagg tgaacacgcc
     1681 ctcgggaccg gccacacgcg tgtccccgta gatcgtgcgc atgcgctcga acaccgcgtg
     1741 cagcaccagc ggcgtgggct gcatcaggcc cggcaggaac gcgggcgcct ccgtgccccg
     1801 ctcggtcacg ctcgtcatcg gtcccctccg tgtcccgcct cgccgtccgc ccgctcggcg
     1861 aactcggcca cgtccatcgc cgtcagcccc agcaccagct gcgccgcctc cgccggcacg
     1921 cgcgggtcca gcccggtcag ctcgcgcacg cgcttgaggc ggtaggccac ggtgttcacg
     1981 tgcacgcagt cctcgcgcgc ggtctccggc acggagccgc acgccaggta gcggcgcagc
     2041 gtgcccagca ggtgctcgtc gccgcgctcg cggaacacgt ccatgacctt gtggcgcagc
     2101 acttccgcca ggcgcgggct gtccgtcacg agctgctcca gcagcaggtc gtcgggcgcc
     2161 agcacgccgc cgtgccgccc ggcggcggcg gcggcctcca gcagcgtctc ggcgcgcgcc
     2221 acctcggtgg ccagcacggg gccgggcggg gcggcctcgc cgcgcgccgc cagccgggcg
     2281 ccgacggcgc gcgccagcgc ggggatgtcc gcgccgggcg cgggcaccag ggcgagcagg
     2341 cggtgcccgc gtgtgccggc cagcgtgtgc ggcggcgcgg cgtcgagcag ccggtcgatc
     2401 aggcccgagg agtggtcgtc ctcgacgagc atcggcacgt aggcgcgcgc gagcgtcacc
     2461 gaccagcggc tggccaccgc ggccagctcg gcggcgtcgc cggctgccag caccgcgtcg
     2521 agcacggcgc gccggcgtgc gtcggcgcgg cgaagctgct cgtgcgaggc ggacaggtag
     2581 gcctcggcga acgcggagga gatgcggtcg acgtagtcga tccagtcgcc ggccaggcgc
     2641 gccaggacgt gctcctcgcc gggctgggtg gcctccacca cggcgttcca gaccacgcgg
     2701 ccggcgatgc ggaacgcgtg cagcgccgag tccagcggca cgccgatctc cagccgccgc
     2761 cgcccggcgg cacggaagtc caccggcacg gtggggtcgg gctcgccctc ggtgaggatc
     2821 gcggagaaga actcctccac cacctcacgc gacaccgggg cgacctccgc ctccacgacg
     2881 ccggcgtcca gcgccccgta ctcggggatc tcctcgcggt aggcggtggt catgcgcggc
     2941 agcagctcgt cgatggtggc cagcacgcgg cgcgccacct ggcgggccgc ctcggggtcg
     3001 tggcggaaca ggccgccgac gtcctggccg cgtgtcgcgt ccggcgtcat cggcccactc
     3061 cgtggtcggg gtccagcgac gccgacatca gccgcccgat gccaagcagc gacgccaggt
     3121 tgtcgcggtc cagctcgtcc acgggcgcgc tggcgaccac gcggccgtgc tggagcacct
     3181 cggcgcggtc ggcgaccgcg agcgcgaacg cgatgtgctg ctccaccagc aggatcgtca
     3241 atccgtcgcg gttcacgtcc gtcaagagct cggccagctc ctccacgatc gcgggcgcca
     3301 gtcccgccgt gggctcgtcg accagcagca gcttcggctc gttcaccagc gcgcgcgcca
     3361 ccgcgagcat cgcctgctcg ccgccggaca gcgtgccgcc gagctgcctg cgccgctccg
     3421 ccagcctggg gaagcgctcg tacagcccgg cgacgacgcg gtcgtagcgt gcgcggtcgg
     3481 cgcggcgcag caggtaggcg cccaggcgca ggttctcctc cacggtgagc tgcgggaaca
     3541 cctgccggcc ctgcggcacg tgacccatgc cggcgccgac gcgccgctcg ggggacaggg
     3601 gcgtgacgtc ctcgccgtcc aggcgcacct ggcccgacca ggcgggcacc agcccggcga
     3661 tgaccttgag cgtcacgctc ttgccggcgc cgttgaggcc gagcacggcg gtgatggagc
     3721 cgggctccac gtcgaagccg acgccgtgca ggacggcgtt cgcgccgaag ccggcgcgca
     3781 ggccgctgac gcgcagcagc ggcggcggct gcgacggcgg tcgcacggac gtgtgtgcgc
     3841 tgacggcgct cacggcgcca cctccgccgc cttggttttc gcggcgaagc cagcacccag
     3901 gtaggcgtcg agcacggcct ggtcgcgcac gacctcgtcg gggccgccgg ccgccagcac
     3961 ctgcccgagg ttgagcacca ccacctcgtc gcagacgtcc atcaccagcg ggatgtggtg
     4021 ctcgatcagc aggacggtgc ggcccaggtc gtcgcgcagc tcgcgcaggc gctcggcgag
     4081 ctgctccacc gcggcggggg ccatgccggc cgacggctcg tccagcagca gcacctccgg
     4141 gtcggtcacc agcgcgcagg ccagctcggc gatgcgctgc tggccgccgg acaggtgctt
     4201 gaggggcagg tccgcgtgcg cggtcatgcc cagcgcggcg acgacctcgc ggctgcgcgc
     4261 ctccaggtcc cgctcggcgc gcgcgacgcc gccggtgcgc agcagggccg gcgcggtggc
     4321 gtagcccagg agccggtgct gggccagcat cacgttgtcc aaaagcgaca ggccgcgcgc
     4381 gagtcccgcg gtctggaacg tgcggcccag gcccagcagc gcgcgctcgt gcggcggcag
     4441 cccggccagc tccctgccgc gcagcgacac gctgccggac tcggcggcca cgaagccgga
     4501 gatggcgttg aacagcgtgg tcttgcccgc gccgttggga ccgatcaggc cgacgatgcg
     4561 gcccttgggc acttccagcc cggcgccgtc cacggcgacg aggccgccga agcgcacgcg
     4621 cacgtcgcgg acggccagga ccacctcgcg cccggccgac ccggcgtccg cggcggcgag
     4681 ccgggccccg gcggcatggg tgaacgccgg caccgcgccc tccgcggcgg ggccgccgcc
     4741 ctggacggcg cgcgcgtgct cggccgcctt tttctccctg gcctcgcgca gcgcgccggc
     4801 gaagcccgtg gggtggcgcg ccatggtgaa caccagcagc gccgagccga tgacgaggtc
     4861 ccagccctcg aacaccgtga gcatgcgcgg gacgatcccg aacagcgcgg cggccaccac
     4921 cacgccgacg cggctgccca ggccgccgac caccacgaac gtcaccaggg tgagggagaa
     4981 caggaagctg aagttgcgcg actccacgaa cagcagcagg tggccgtaca gcgcgccgga
     5041 cacgccggcc agcgcgccgg agatcacgaa cgcgagcagc ttgtagcggg agacgtccac
     5101 gccgtatgcc tgcgccacct gctcgttctc gcgcaggccc tggaacgccc gccccagctt
     5161 ggagcgggtg atgttgccgt ccagcagcca gaagcccagc acgaagacga gcaccagcgc
     5221 caggtagtcg gggttgcggg tgaacacgaa cgcgccggcc gccggccgcg gcagggacac
     5281 gccggcggag ccgccggaca cccacggcag gcggaacacg gcctgctcca tggccaccgc
     5341 gaagccgatg gtggtgatcg ccaggtacag gccgcgcagg cgcagggcgg gcagcccgac
     5401 caggaacgcg gccgccgcgc ccaccagggc cgcgaccacg acgccggcga gcatgggcag
     5461 gccggcgcgg ccggtgagga tgccgctggc gtaggcgccc gcgccgatca gcgcggcgtg
     5521 gcccagcgag atctgccccg cgtagcccat cagcacgttg agcgacagcg ccatggcggc
     5581 gtagcaggcg ccgaagccca gcatcacggc gcggaagtcg gtgagcacgc ccaggcggcc
     5641 gagcagcggc ggcaggatcg cgaacgcggc caggaccagc agcgcgcccg ccttcccgcg
     5701 cgggag
//
LOCUS       DATMKC010000045         2909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_165570, whole genome shotgun sequence.
ACCESSION   DATMKC010000045 DATMKC010000000
VERSION     DATMKC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165570"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1509)
                     /locus_tag="VNU01_01215"
     CDS             complement(295..1509)
                     /locus_tag="VNU01_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWH31269.1"
                     /translation="MSEAPGDPVARGDQGGGSGDPVMDVHAGGKISVATKVAVNDRND
                     LALVYTPGVAQVCMAIHADPSLVHSLTIKRNSVAVVTDGTAVLGLGDIGPAAALPVME
                     GKAMLFKDFADVDAYPICLDEKDPDKIVDAIVALAPGFGGINLEDISAPRCFEIEGKL
                     RQRLDIPVFHDDQHGTAVVTLAALFNAVRVVHKALADLKVVISGAGAAGVAITRMLQS
                     AGVSDIVVTDSVGIVSSARKNLTAPKQWLAKHTNPRSLDGPIAKAMLGADVFIGVSQP
                     GAITRDDVRNMAPGSIVFAMANPVPEVGPEDVEGLAAVYATGRSDLPNQINNVLCFPG
                     IFRGALDVQATEINEAMKLAAAHAIAEAVPAGDIGPGTVVPSVFHPGLGHTVAARVAE
                     AAVATGVARLAP"
     gene            complement(1638..>2909)
                     /locus_tag="VNU01_01220"
     CDS             complement(1638..>2909)
                     /locus_tag="VNU01_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH31270.1"
                     /translation="LLLTSPDALDARTAAELRRLRPARVLVLGGESAVGEDVARAVAD
                     LGVPVTRLSGATRVETAVAIAEDQAPTAGTAILARAWPREGEAGSAMFTDAMVAGGWS
                     ASRGWPVLLTGTGAVPDATAAYLERAGVRTVHVVGGSAAVSDAAAAQLAAMGVSIARV
                     SGGTRFATAAAVATARGETRRFVLVDGEGGGAWAAGFAAAAYSARFDAPILPVAADRL
                     DDSVARLLRGDGRRSLVCGPAVSADVCGSAAREAGQVLRAEAALVADAEGVAGAVTNP
                     EGIAAIKVIGCGVEHTVQPDASGQFTLPVSSGDCRLTVRIEHTDGTAFDQAFTLAGGT
                     VRAHPKAAPVPGLPRGGLGLPTGGTDDSGDDLDGGLLPGSEEPTSEPTAEPTAEPTAE
                     PTAEPTADPDPLLECSAALCGGGGTNNVEMM"
BASE COUNT          361 a         1123 c         1054 g          371 t
ORIGIN      
        1 cggacggggt ggggttcgcg gtcgggctgg gcgaggggct cgccgtgcgt gtcggcgtcg
       61 gcgatggcga cggcgacggc gactcctcga cataggacac cggcacgagt gcggccggtg
      121 cggctgccga ggcgaagacg gcggcggcca gcaccgcccg gacgagcgcg ccgatccccc
      181 acgcgttcgc cgacccggcc ttcctggtgg tccgacccag gcctgggctc atcctcatac
      241 cccccgatac gcgtgggcgc ccgccgcgct tacgcggcga attgcccggt tccgtcaggg
      301 cgccagccgc gcgacccccg tggccacggc cgcctcggcg acacgggctg ccaccgtgtg
      361 gccgagcccc gggtggaaca ccgacggcac caccgtgccc ggcccgatgt cccccgccgg
      421 cacggcctcg gcgatcgcgt gggcggcggc cagcttcatg gcctcgttga tctccgtggc
      481 ctgcacgtcc agcgctcccc ggaagatgcc cgggaagcac agcacgttgt tgatctggtt
      541 gggcaggtcg ctgcggcccg tggcgtacac ggccgccagc ccctccacgt cctccggccc
      601 gacctccgga acggggttgg ccatcgcgaa cacgatcgag ccgggtgcca tgttgcgcac
      661 gtcgtcacgg gtgatggcgc ccggctgcga cacgccgatg aagacgtcgg cgcccagcat
      721 cgccttggcg atcggaccgt cgagactgcg cgggttggtg tgcttggcca gccactgctt
      781 cggcgcggtg aggttcttgc gcgccgagct gacgatgccc acgctgtcgg tcaccacgat
      841 gtcgctgacg ccggcggact gcagcatccg ggtgatcgcg acgcccgccg cccccgcgcc
      901 ggagatcacc accttcaggt cggccagcgc cttgtgcacc acccgcacgg cgttgaacag
      961 ggcggccagg gtcaccacgg cggtgccgtg ctggtcgtcg tggaacaccg ggatgtccag
     1021 ccgctggcgc agcttgccct cgatctcgaa gcagcgcggc gccgagatgt cctccaggtt
     1081 gatgccgccg aacccgggcg ccagcgcgac gatcgcgtcg acgatcttgt ccgggtcctt
     1141 ctcgtccagg caaatcgggt aggcgtcgac gtcggcgaag tccttgaaga gcatcgcctt
     1201 gccctccatg accggcagcg cggccgccgg cccgatgtcg cccaggccga gcacggcggt
     1261 gccgtcggtg acgacggcga cgctgttgcg cttgatcgtc agggagtgga ccagcgacgg
     1321 gtcggcgtgg atcgccatgc agacctgcgc gacaccgggc gtgtacacca gcgcgagatc
     1381 gttgcggtcg ttgaccgcga ccttggtcgc cacgctgatc ttgccgccag cgtgcacgtc
     1441 catgaccggg tcaccggacc cgccgccctg gtccccccgt gcgaccgggt cgcccggcgc
     1501 ctcgctcatc gcctcgcctt cctgcttgcc gcctgccgtc tcgcggcgtc ggggccacgg
     1561 gatatccgac aagcatgcgg tctgcgcacg gcgcggcgca aggcggacgg acggccaggg
     1621 cgcgcccgtc cgtcagctca catcatctcg acgttgttcg tgccaccgcc gccgcacagc
     1681 gcggccgagc actccagcag cgggtcaggg tccgcggtcg gctccgcagt cggctccgcg
     1741 gtcggctccg cggtcggctc ggcggtcggc tcggacgtcg gctcctcgga gccgggcagc
     1801 aggccgccgt ccagatcgtc gcccgagtcg tccgtgccgc ccgtgggcag gcccaggccg
     1861 cccctgggca agcccggcac cggagcggcc ttcgggtgcg cgcgcaccgt gccgcccgcc
     1921 agcgtgaacg cctggtcgaa cgccgtgcca tccgtgtgct cgatgcgcac cgtgagacgg
     1981 cagtctccgg agctcaccgg gagcgtgaac tgccccgagg cgtccggctg cacggtgtgc
     2041 tccaccccgc agccgatgac cttgatggcg gcgatgccct ccgggttcgt caccgcgccg
     2101 gccacgccct cggcgtcggc gaccagggcc gcttcggcgc gcagcacctg gccggcctca
     2161 cgcgccgcac tgccgcacac gtccgcgctc accgcgggac cgcacaccag cgagcgccgg
     2221 ccgtcgccgc gcagcagccg ggcgacggag tcgtcgaggc ggtcggccgc caccggcagg
     2281 atcggcgcgt cgaagcgcgc ggagtacgcc gcggcggcga agcccgccgc ccaggcgccc
     2341 ccgccctcgc cgtccaccag cacgaagcgg cgcgtctccc cgcgcgccgt ggccacggcc
     2401 gcggcggtgg cgaaacgggt gccgccggag acccgcgcga tggacacgcc catggcggcc
     2461 agttgggcgg cggcggcgtc cgacaccgcc gcgctgcccc ccaccacgtg gacggtccgc
     2521 acgcccgcgc gctccaggta ggcggccgtg gcgtccggca cggcgccggt gccggtcagc
     2581 agcaccggcc agccgcgcga ggcggaccag ccgcctgcga ccatcgcgtc ggtgaacatc
     2641 gccgagccgg cctcgccctc gcgcggccag gcacgcgcca ggatcgcggt gccggccgtg
     2701 ggcgcctgat cctcggcgat ggccaccgcc gtctcgacgc gggtggcgcc cgacaggcgc
     2761 gtcaccggca cgcccaggtc cgcgacggcg cgcgcgacgt cctcgcccac cgcggactcg
     2821 ccgccgagca ccaggacgcg ggcagggcgc aggcggcgca gctccgcggc ggtgcgcgcg
     2881 tcgagggcgt ccggcgaggt cagcaggag
//
LOCUS       DATMKC010000046        14121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_113406, whole genome shotgun sequence.
ACCESSION   DATMKC010000046 DATMKC010000000
VERSION     DATMKC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113406"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VNU01_01225"
     CDS             <1..469
                     /locus_tag="VNU01_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31271.1"
                     /translation="GSCTMSVPAGSYVLVQTGAPGGLEGIEPVPFTFASGGQVAAVTV
                     TNYPPQDAVAPEPQPGPVYSPPVDTAPPVESVTDFEPVPVVEGPAPRVSVPERVGGTV
                     VQVIRAPGDALRLLSRDPKQAVAWTAAIMLLALAVIAVRRRQHALALIGTDAS"
     gene            741..1457
                     /locus_tag="VNU01_01230"
     CDS             741..1457
                     /locus_tag="VNU01_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31272.1"
                     /translation="MAFFLVVAMALGVGIMPAAADEPRAPDAGVFQGTAQVGKSGDCD
                     NEGGAHNVTGGGIGAPVVNGPKNAQYSINAPDSVVSVSYGVGSLRLCGFLTGPLGIKK
                     VVVDDAKATSENDLTVPVGASCVSTKGWGGKGKATFNSVLGEPATTVIYISNLGWKAT
                     VGGTFLVTADTGGTSKKKADLLVAVVQALSEGAVIGCLEKVLAMQDKSGLTPDPFTVV
                     ASYALLPVENPQDKKPPKQA"
     gene            1762..2409
                     /locus_tag="VNU01_01235"
     CDS             1762..2409
                     /locus_tag="VNU01_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31273.1"
                     /translation="MKLRASALFLAVSMMLGLAALPAAAAVDSDLGVFQGTAQVGKSG
                     TCDNSGGAHDVTGGGIGLPVVNGAKRAQYSINAPSSVVSPIYGIGSLRLCGKLTSPLD
                     IEYVEGTPVGASCASTKGYDGQGKATFDGLVKDRDVYLKNLGWEITVGGTFLVTADGG
                     GSKGADSHKLVAVVQALSEGAVVGCLEKTLAGMDKSGLQPDPFTVAAAYVLVPVP"
     gene            complement(2461..2805)
                     /locus_tag="VNU01_01240"
     CDS             complement(2461..2805)
                     /locus_tag="VNU01_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWH31274.1"
                     /translation="MPKPKKFVFVCLNQRDPAHARPSCAARGGGDVFAALRDEQGLRR
                     NTDVKIVAGGCLEACMVGPVIAVYPDDIFYGGITEADVPAIWDHLEGGEPVAPLVLQG
                     GEWDLRPPALEA"
     gene            2825..3580
                     /locus_tag="VNU01_01245"
     CDS             2825..3580
                     /locus_tag="VNU01_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH31275.1"
                     /translation="MDLGLKDKVALVAGGSSGLGLAFAKELAAAGAGVSIASRSAERL
                     ADAAREIGGDVHTATVDVRDQAAVRAWVDAAAECFGAVHIVFANAGGPTPGPATAFDV
                     ADYREAIEVNLLGSIALVQTALPHLKAAGWGRVLFVTSQGVRRPIPHLALSNTARPGI
                     LGYAQSLVHDLADSGITVNVLAPGLHRTARLEQLAGDDEQALAAMAQSVPLGRIGAPE
                     EFAAVAAFLASERASYVTGAVVPVDGGSTGLLL"
     gene            complement(3581..4477)
                     /gene="folP"
                     /locus_tag="VNU01_01250"
     CDS             complement(3581..4477)
                     /gene="folP"
                     /locus_tag="VNU01_01250"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWH31276.1"
                     /translation="MEPLPVPLPALRFRLGARKWDLSRRVLVMAIVNRTPDSFYDRGR
                     TYELGKAVEHALAQVEAGADIIDVGGVKAGPGAFVDAAEERERIVPFVEAFRARSDAP
                     LSIDTFRAAVAAEALDAGADLVNDVSGMVEPEVADVVAARPGTALVVMHAGGPVRTRP
                     FRPDYRPDVTTAVVETCRRLAGEAQRRGVPRESIVVDPGHDFGKTTEQSLEVTRRLPE
                     LAALGFPVLVALSNKDFLGETLGLPVEERVEASLACAAASVLLGARMVRVHETLQTVR
                     AVRTVEAVLGWRPPAAAARGLD"
     gene            4525..4980
                     /locus_tag="VNU01_01255"
     CDS             4525..4980
                     /locus_tag="VNU01_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31277.1"
                     /translation="MLVAAVTTLLGAVVVAAALLRDEPMTPRGAAGARPAWQGLPEPA
                     DLARTSFPQALAGYDPASVDVHFERMLRAYEDLWAVASPELRERARMRAASRSGAPPE
                     QAETHARDDDVTAVAVPEPPDLGETDADALAAEAALARVETDEDGGGRA"
     gene            4977..5942
                     /locus_tag="VNU01_01260"
     CDS             4977..5942
                     /locus_tag="VNU01_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31278.1"
                     /translation="MSDGEPQPSRQGAQASGAADGLSLPPPPAQRRGGIVRRSLAVAR
                     EHWWTAGAAAAVLLMPMSFVSAFVDRAWFDSRFVADAMLDGGSGPRGNPLLGFVEAVI
                     VTPWSLAAALLVVIGRVAAVEGAVAVALRRLPQAVGAMFLAAVVAALPLLPAAALFVL
                     NVDEAVAVMGPGGTVNPEEFPAGMVLAMLLLVLGLVPTVFLGLTLGLAVPLAVADGLG
                     PVAACKASWRRMPGQRLRLLGGMLAVVLPALFVLSLLALPLGLLALSLGREGGWVAAG
                     VGDGMLMSAVTVLLVSIYAVLRADTAPAEPLPPGDPEARWEPDPS"
     gene            complement(5939..6709)
                     /locus_tag="VNU01_01265"
     CDS             complement(5939..6709)
                     /locus_tag="VNU01_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH31279.1"
                     /translation="MFIELHGARHSYVSKGEGPPIVFVHGLGGTQHVWHGVIDALSLH
                     HHCVALDLRGHGRSGGAGTFSVKGWAQDVEALIAALELPPVTLVGHSMGTLIAQQLAH
                     SRPELVDNLVLVGGISYFEPPTKDAYEQRADLVEAEGMDALVDDWLPGALSPRNAAKL
                     PQLVGLLREVFVRNDPASYAKSCRAIAKAPVIARTDIGQPTLLLVGDHDRSTPIAMTE
                     ELHREIPVSMVRVIPNAAHWVPLEQPDAIAAAILEFLT"
     gene            6858..7316
                     /locus_tag="VNU01_01270"
     CDS             6858..7316
                     /locus_tag="VNU01_01270"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH31280.1"
                     /translation="MARIEEHVRIHAPAARVWGVLTDWEGQARWMRDARSVRVLTERR
                     EGRGVVLRCATDLFGLVVDDDMEVTEWEHQRLIGVRHLGMLIRGVGAFELEPLPGGAT
                     RFTWWEEVEAPFGAVGDALAALIVVPHVSRVFRGSLAALKAVCEGEEPAA"
     gene            complement(7324..9927)
                     /locus_tag="VNU01_01275"
     CDS             complement(7324..9927)
                     /locus_tag="VNU01_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31281.1"
                     /translation="MRIHRLRLANYRGVAAREVAFAERGVTVVQGPNEAGKSSLAEAL
                     DLLLEHYDDSRKAGVRATKPVGADVGTEVEAELSAGPYRFTYAKRFHRERFTTLTVHE
                     PRPEQFTGREAHERVRAILAETVDLPLWRALRMVQGQAVEQPDLSEVSALAAALDVAA
                     AGARGGEAEHTLLDRAQAEYLRSFTPTGQDNAWFKGLRAELDAAEAEAAEARRALAGL
                     ERDVEDAARIAGELEALRPELQRRRLLLEDLERRWTAVRERAAEVERLALRAERAEAA
                     AQGAAREIAERARLTEAAEATAAAAEEALREQDASAPALEAAAAEEQRLQEQVTGLRA
                     ELSAARDAVRAALDAEARSKEAFSLELLEERWARVEQARAQAAQAREVLEDARVDEGA
                     AAEIERAQLALAQAQARLADAGPLLRLEARQDVAVELDGQALDLAAGARHELPVTEEV
                     RLRVGGLDAVLSPGQGLSALRDRLAEAERAWREACARAGVADLAEARGALRRRDDAER
                     ALAEADRVLRDNLRDLSPDELHGKAVRLRERLAAADAGADVDAARGAREHAERRVGEL
                     QDALEHAESALRETSARRAALRERHTGLALRAEQALAAREAAEAALAAARGAAADAGL
                     GKRAAALAAEAAEAAQAAAAARASLEGEDPATLQAEVDNAREAAEKQRRELREREDEL
                     TTLRGRLSALGEVGLHDRLAAAETRLERAGTAWRQAERGAAAARLLYETLLRHREAAR
                     RAYVAPLREKLESLGRIVFGQDFAVELDDDLRVARRTLDGTTLDVEQLSTGAREQLAV
                     LARLAVAAITAADGGVPVLLDDALGWSDPQRLERMAAAFHVAARDSQVIVLTCVPERY
                     AAIGSATTVRL"
     gene            complement(9924..11048)
                     /locus_tag="VNU01_01280"
     CDS             complement(9924..11048)
                     /locus_tag="VNU01_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HWH31282.1"
                     /translation="MRFLHTGDWQLGMTRAFLTDEAQARFGAARLEAVREIGRVAQRE
                     GCAFVLVCGDVFESNHLDRQVLLRALDVMGGLGLPVYLLPGNHDPLDAGSLFTSPEFA
                     RARPANVTVLDRAGAWPVAPGVELVTAPWTSKRPLRDLVAAACEDLRADGTVRVVAGH
                     GCVDVLSPDADDPAVIGLAPLERALADGRVHYVALGDRHSLTELGAGGRVRYAGAPEP
                     TDFDEVAPGHVLVVDVDARDCRAEAHPVATWRFAQPEPFALRDADDVSVLATWLAEQP
                     DKERCAVRLRLVGSLGLRDMALLDDVLDRHRELFASLRVWERHSDLALLPEEDDLAEL
                     ELAGFAADAVGDLRAAAEGGADAAQDALALLYRLHRGAAA"
     gene            complement(11124..11564)
                     /locus_tag="VNU01_01285"
     CDS             complement(11124..11564)
                     /locus_tag="VNU01_01285"
                     /inference="COORDINATES: protein motif:HMM:NF022793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HWH31283.1"
                     /translation="MSLRIAGEDAPEPTLLPEEPQEARHALAQALGGDVPDIAVREVA
                     ASYPTFLEAWAILAERALHAGDPVAAYAFARTGYHRGLDKVRRAGWRGSGPVPWSHEA
                     NRGFLRSVHALGRAAWAIGEHEEADRCAVFLRELDPEAARVLED"
     gene            complement(11561..13066)
                     /locus_tag="VNU01_01290"
     CDS             complement(11561..13066)
                     /locus_tag="VNU01_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWH31284.1"
                     /translation="MADYRLFIDGEFRDAASGETFQTLDPSTGESLGTVARAGREDAQ
                     AAIAAARAAFDDGRWSGRTGAERGAVLRAAADLIAAQAGELAELERRDSGSTLVKAMA
                     ADVGGAIQWFSACADMAATLDEPETLPPSPFGSANTLRRDPVGVCSAIVPWNFPLQMA
                     AWKLAPALAAGNTVVLKPAEETPCTAVRLAEILREAGVPDGVVNVIPGPGPGTGEELA
                     SNPDVDKVAFTGSTNVGREIMRMASPTLKKVTLELGGKSAQIVLDDADLDLAVDGALY
                     AVFLHSGQACTAGSRLLLPDALHDEFVGRLLERASAIRVGPTADPSNQLGPLVSQKQL
                     DTVLHYIDIGGKEGATLALGGSRLTDGELAQGFFVEPTVFTDVRADMTIAQEEIFGPV
                     LSVLRYRDEDEAVAIANGTAYGLAAGVWGSPARATALAGRLRAGTVWVNEWHLLNPHY
                     PFGGYGQSGIGREHGLLGLHEWTEAKHVHAASDTSRAAHRWFDMVVPGSTA"
     gene            complement(13099..13539)
                     /gene="paaD"
                     /locus_tag="VNU01_01295"
     CDS             complement(13099..13539)
                     /gene="paaD"
                     /locus_tag="VNU01_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HWH31285.1"
                     /translation="MSAPTPDAVRAALAGVPDPELPALSVLDLGIVAAVRVEGAAVEV
                     DLVPTFTGCPATQVIRDEVEAALRAMPGVADARVRWVTAPVWSPARISAEGRTALKAF
                     GVSMDAGCPHCDSARTDQESAFGPTPCRALRFCEDCRNSFEVMK"
     gene            complement(13536..>14121)
                     /locus_tag="VNU01_01300"
     CDS             complement(13536..>14121)
                     /locus_tag="VNU01_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HWH31286.1"
                     /translation="LAADADGRDPDILALGRAPEDYRSCALVERPNGDWAYTVARHWL
                     ADTAETARLEWLAASGWPELAGAAAAPLREQAWHVRHAEEWVGRLAAAGGEAVARLRA
                     ALERALPEAPGLFAPSVGEDTLVADGTLPCASAAVLAAWRARRRADLDGWGLGELGGL
                     LDAEPGDRTARTPEFAPLWEELTGLHRAHPGAAW"
BASE COUNT         1704 a         5312 c         5197 g         1908 t
ORIGIN      
        1 tggcagctgc acgatgagtg tgccggcggg cagctatgtg ctggtgcaga cgggcgcgcc
       61 tggcgggttg gaggggatcg agccggtgcc gttcacgttc gcgtcgggtg gtcaggtggc
      121 ggcggtgacg gtgacgaact atccgccgca ggatgcggtg gcaccagagc cccagcctgg
      181 gccggtgtat tcgccgccgg tcgataccgc gccgccggtg gaatcggtga ctgatttcga
      241 gccggtcccg gtggtggagg ggcccgcgcc gcgcgtgagt gtgccggagc gtgtgggcgg
      301 cacggtggtg caggtgattc gcgcgcctgg tgacgcgctg cgcctgctgt cccgtgaccc
      361 gaagcaggcc gtcgcatgga ccgccgcgat catgctgctc gcactcgccg tcatcgccgt
      421 ccgacgccgc caacatgcgc tggcgctaat cggaactgac gcgtcgtagt tgtcggcagc
      481 cgagtcctct gcggtatcac acgccggaag agcagtcgcc cggccgggcg ccctcggtcg
      541 ctgggcagcg attgatctgg agcttgatga ttcggtgcaa catgatctag tgtgacaccg
      601 tacgccgcag cagggggcta cggcgcgggg gatcagtctt ggggcgacgt cggaatcctg
      661 cgctgtgtcg agcgtggtcg cgatgtcgtg tgcgctggtc cttctctcaa ggaaggagcg
      721 caacaatgaa actgagaggt atggcatttt tcctggtggt cgcaatggcg ctcggcgtag
      781 gcatcatgcc tgcggcagcc gacgagcctc gtgctcccga cgccggtgtc ttccagggca
      841 ccgcgcaggt gggcaagtcc ggggattgtg acaacgaggg cggtgcccac aatgtgaccg
      901 gtggtggtat cggcgctccc gtggtgaacg gtccgaagaa cgctcagtac tcgatcaacg
      961 ccccggacag cgtcgtctcc gtcagttacg gggttggttc cctgcgcctc tgcggcttcc
     1021 tcacgggccc gctgggcatc aagaaggtgg tggtcgacga cgcgaaggcg acttcggaaa
     1081 acgacctcac cgtgccggtc ggggcctcct gcgtctcgac caagggttgg ggcggcaagg
     1141 gcaaggccac cttcaactca gtgcttggag agccggcgac caccgtgatc tacatctcca
     1201 atctggggtg gaaagcgacg gtcggcggca ccttcctcgt gactgccgac actggcggca
     1261 cgagcaagaa gaaggcggac ctgctcgtcg cggtcgtaca ggcgctgagc gagggcgccg
     1321 tcatcggatg tctcgagaag gtgctcgcca tgcaagacaa gagtggcctc acgccggacc
     1381 cgttcacggt cgtggcgtcc tacgcgttgc tgccggttga gaacccgcag gacaagaagc
     1441 cgccgaagca ggcttagcca cgtgaagccg taggcaagta ccacaggggc cctgacaaaa
     1501 cgtcggggcc ccttcctcat gcccgcgtcg gcgaccgaca cgccaaccgt cccgcggcgc
     1561 ccgtcgcatg acgctgagaa gcggatattt ggttaagctc ttgatttccg gttgcgccac
     1621 ggcttaatgt gacacggcgt gcagcagggg gctgccgcga acggtgggat cagcgggggc
     1681 cgacgggggg atcgttgcag tcgaacatgt ctcctccgtc gtgaacgcgc gatccctttt
     1741 ctctacgaaa aggagcgcaa catgaagctc agggcatccg ctctgtttct ggctgtgtcc
     1801 atgatgctgg gcctggcggc ccttccggcc gccgcggctg tggactccga tcttggcgtc
     1861 ttccagggca ccgcccaggt cggcaagtcc ggcacctgcg acaactccgg cggcgcgcat
     1921 gacgtgaccg gtggtggaat cggccttccg gtggtcaatg gcgccaagag ggctcagtac
     1981 tccatcaacg cgccgtccag cgtcgtgtcg cccatctacg gcatcggttc cttgcgtctg
     2041 tgcggcaagc tgacctctcc gctcgacatt gaatatgtcg aaggcacgcc cgtcggcgcg
     2101 tcgtgcgcct ccacgaaggg ctatgacggt cagggcaagg ccaccttcga tggtctcgtg
     2161 aaggaccgcg acgtctacct gaagaatctt gggtgggaga tcaccgtcgg cggtacgttc
     2221 ctggtcacgg ctgacggcgg tggcagcaag ggcgccgact cccacaagct tgtcgccgtc
     2281 gtgcaggccc tgagcgaggg cgcggtcgtg ggctgtctcg agaagaccct ggccggcatg
     2341 gacaagtccg gcctgcagcc cgacccgttc accgtcgctg cggcctacgt gctggttccc
     2401 gtcccgtagg gatgctctcc atgggaaggg ctccggcagc gccggagccc ttcctgccgc
     2461 ttaggcctcc aacgcgggcg ggcgcaggtc ccactcgccg ccctggagta ccaggggcgc
     2521 gaccggttcg cccccttcga ggtgatccca gatcgccggc acgtccgcct ccgtgatccc
     2581 gccgtagaag atgtcatcgg gatacaccgc gatcacgggc ccgaccatgc aggcctccag
     2641 gcagccgccc gcgacgatct tcacgtcggt gttgcggcgc aggccctgct cgtcgcgcag
     2701 cgccgcgaac acgtcgccgc cgccgcgcgc cgcgcagctc ggccgggcgt gcgcggggtc
     2761 gcgctggttg aggcacacga acacgaactt cttcggcttt ggcatgcggc tagcctacgc
     2821 agccgtggac ctgggactga aggacaaagt cgcgctcgtc gccgggggca gctccggcct
     2881 cggcctggcg ttcgccaagg agctcgccgc cgccggcgcc ggtgtctcca tcgcgtcgcg
     2941 atccgccgaa cgcctcgccg acgccgcccg cgagatcggc ggcgacgtgc acaccgccac
     3001 cgtggacgtg cgcgaccagg cggcggtgcg ggcctgggtg gacgccgcgg ccgagtgctt
     3061 cggcgcggtg cacatcgtgt tcgcgaacgc gggcgggcca acgcccggcc cggcgacggc
     3121 gttcgacgtc gccgactacc gcgaggccat cgaggtcaac ctgctcggct ccatcgccct
     3181 ggtccagacc gccctgccgc acctgaaagc ggccggatgg gggcgcgtgc tgttcgtcac
     3241 ctcgcagggc gtgcgccgtc ccatcccgca cctggcgctg tccaacacgg cccgccccgg
     3301 catcctcggc tacgcccagt cgctggtgca cgacctcgcc gacagcggca tcaccgtgaa
     3361 cgtgctcgcg cccggcctgc accgcaccgc ccgcctggag cagctcgccg gcgacgacga
     3421 gcaggcgctg gccgcgatgg cgcagtccgt gccgctcggc cgcatcggcg cgccggagga
     3481 gttcgcggcc gtggcggcgt tcctggccag cgagcgcgcg tcctacgtca cgggcgccgt
     3541 cgtcccggtc gacggcggct cgacgggcct gctgctgtag tcagtccagg ccgcgcgccg
     3601 cggccgcggg cgggcgccag cccagcaccg cctccaccgt ccggacggcg cgcaccgtct
     3661 gcagcgtctc gtgcacccgc accatgcggg cgcccagcag cacggaggcg gccgcgcacg
     3721 ccagcgacgc ctccacgcgc tcctcgaccg gcagccccag cgtctcgccc aggaagtcct
     3781 tgttcgacag cgccaccagg acgggaaagc cgagcgccgc cagctccggc agccggcggg
     3841 tgacctccag cgactgctcg gtggtcttgc cgaagtcgtg gcccgggtcc accacgatcg
     3901 actcccgcgg cacgccgcgg cgctgtgcct cccccgcgag ccggcggcac gtctccacca
     3961 cggccgtggt cacgtccggc cggtaatccg gccggaacgg ccgggtgcgc accggaccgc
     4021 ccgcgtgcat caccaccagg gccgtgcccg gccgggccgc gaccacgtcg gcgacctccg
     4081 gctccaccat gccgctgacg tcgttgacca ggtccgcgcc ggcgtccagc gcctcggccg
     4141 ccacggccgc gcggaacgtg tcgatggaca gcggcgcgtc gctccgcgcg cggaacgcct
     4201 ccacgaacgg cacgatgcgc tcgcgctcct cggcggcgtc cacgaacgcg ccgggccccg
     4261 ccttcacacc gcccacgtcg atgatgtccg cgcccgcctc gacctgcgcc agcgcgtgct
     4321 cgaccgcctt gcccagctcg taggtgcggc ccctgtcgta gaacgagtcg ggcgtgcggt
     4381 tcacgatcgc catcaccagc acgcggcgcg acaggtccca tttccgcgca ccgaggcgga
     4441 accgaagggc tggcaggggt acggggaggg gctccatgcc cgcggatcgt agaggcgcgc
     4501 ggcgcggctg cgatactgcc gggcatgctc gtcgccgcgg tcaccacgct gctcggcgcc
     4561 gtcgtcgtgg cggcggccct gctgcgcgac gagccgatga ccccgcgcgg cgccgccggc
     4621 gcgcggcccg cctggcaggg cctgccggag ccggccgacc tggcgcgcac gtcgttccca
     4681 caggcgctgg cgggctacga cccggcctcc gtcgacgtgc acttcgaacg gatgctgcgc
     4741 gcgtacgagg acctgtgggc cgtcgcctcc ccggagctgc gtgagcgggc gcgcatgcgc
     4801 gcggcctccc gctcgggcgc gccgccggag caggcggaga cacacgcgcg agacgacgat
     4861 gtcacggccg tggccgtgcc ggagccgccc gatctgggcg agaccgacgc ggacgcgctg
     4921 gccgccgagg cggcgctggc gcgcgtcgag acggacgagg acgggggagg ccgggcgtga
     4981 gcgacgggga gccgcagccg agccggcagg gcgcgcaggc gtcaggcgcc gcggacggcc
     5041 tgagtctgcc gccgccgccc gcgcagcgcc gcggcggcat cgtgcgccgc agcctcgccg
     5101 tcgcccgcga gcactggtgg accgcgggcg cggccgccgc cgtgctgctg atgccgatgt
     5161 cgttcgtgag cgccttcgtg gaccgtgcgt ggttcgacag ccgcttcgtg gccgatgcga
     5221 tgctcgacgg cggcagcggg ccgcgcggca acccgctgct gggcttcgtg gaagcggtga
     5281 tcgtcacgcc gtggtcgctt gcggcggcgc tgctggtggt gatcggccgg gtggccgccg
     5341 tggagggcgc ggtggccgtc gccctgcggc ggctgccgca ggcagtcggc gcgatgttcc
     5401 tggccgccgt ggtcgcggcc ctgccgctgc tgcccgccgc ggcgctgttc gtgttgaacg
     5461 tggacgaggc cgtggccgtc atggggcccg gcggcaccgt gaatcccgag gagttccccg
     5521 ccgggatggt gctggcgatg ctgctgctgg tgctcggcct cgtgccgacc gtgttcctgg
     5581 gtctcaccct ggggctggcc gtgccgctgg cggtggccga cggcctcggc ccggtggcgg
     5641 cctgcaaggc gtcgtggcgg cgcatgccgg ggcagcggct gcggctgctc ggcggcatgc
     5701 tcgcggtggt gctgccggcg ctcttcgtcc tgtcgctgct cgcgctgccg ctcgggctgc
     5761 tcgcgctgtc gctcggacgc gagggcgggt gggtcgccgc cggcgtcggc gacggcatgc
     5821 tcatgtccgc ggtcacggtg ctgctcgtgt cgatctacgc cgtgctgcgc gcggacaccg
     5881 cgccggccga gccgctgccg ccgggagacc cggaggcgcg ctgggagccg gacccgtctt
     5941 aggtcaggaa ctccaggatc gccgcggcga tcgcgtcggg ctgctccagc ggcacccagt
     6001 gggcggcgtt cgggatcacc cgcaccatcg acacggggat ctcgcggtgc agctcctcgg
     6061 tcatggcgat gggcgtggag cggtcgtggt cgcccaccag cagcagcgtc ggctggccga
     6121 tgtcggtgcg ggcgatcacc ggcgccttcg cgatcgcgcg gcaggacttc gcgtagctcg
     6181 ccgggtcgtt gcgcacgaac acctcgcgca gcaggccgac cagctgcggc agcttcgccg
     6241 cgttgcgcgg gctgagcgcg ccgggcagcc agtcgtcgac gagcgcgtcc atgccctccg
     6301 cctccaccag gtcggcgcgc tgctcgtagg cgtccttggt gggcggctcg aagtagctga
     6361 tgccgccgac cagcaccagg ttgtccacaa gctccgggcg gctgtgcgcg agctgctgtg
     6421 cgatcagcgt gcccatcgag tggcccacca gcgtgaccgg gggcagctcc agcgccgcga
     6481 tcagcgcttc gacgtcctgc gcccacccct tcacggagaa cgtgccggcg ccgccgctgc
     6541 ggccgtggcc gcgcaaatcc agcgccacgc agtggtggtg caacgacagc gcgtcgatga
     6601 cgccgtgcca cacgtgctgg gtgccgccca ggccgtgcac gaacacgatg ggcgggccct
     6661 cgcccttgct gacataactg tgccgggcgc cgtgcagttc gatgaacatt cgcaggtctc
     6721 caggtggggg tgcgcgcgac gccggaggtg tccgggtgct gcgccgaatc aggtcgtgcc
     6781 gacaggctac ccgacctgac cggacctgcc cggcaccgcc atacttcgca cccgacccga
     6841 ccgccccgga ggccgcagtg gcacgcatcg aggagcacgt ccgcatccac gcgccggccg
     6901 cgcgcgtctg gggcgtgctg accgactggg aggggcaggc gcgctggatg cgcgacgccc
     6961 gcagcgtgcg ggtgctcacg gagcggcgtg agggcagggg cgtggtgctg cgctgcgcca
     7021 cggacctgtt cgggttggtc gtcgacgacg acatggaggt caccgagtgg gagcaccagc
     7081 gcctgatcgg ggtgcgccac ctcggcatgc tgatccgcgg ggtcggcgcg ttcgagctgg
     7141 agcccctgcc cggcggggcg acgcgcttca catggtggga ggaggtcgag gcgccgttcg
     7201 gcgcggtcgg cgacgcgctg gccgccctga tcgtcgtgcc gcacgtgtcg cgggtgttcc
     7261 gtggcagcct cgcggcgctg aaggccgtgt gcgaggggga ggagccggcc gcgtgagccg
     7321 ggcctagagg cgcacggtcg tggccgagcc gatcgccgcg tagcgctccg gcacgcaggt
     7381 cagcacgatc acctggctgt cgcgcgccgc gacgtggaac gccgcagcca tgcgctccag
     7441 gcgctgcggg tccgaccagc ccagcgcgtc gtccagcagc accggcacgc cgccgtcggc
     7501 ggcggtgatc gccgcgaccg ccaggcgcgc cagcaccgcg agctgctcgc gggcgcccgt
     7561 ggaaagctgc tcgacgtcca gcgtggtgcc gtccagggtg cgccgcgcca cgcgcaggtc
     7621 gtcgtccagc tccacggcga agtcctggcc gaagacgatg cggccgaggc tttccagctt
     7681 ctcccgcagc ggcgccacgt aggcgcggcg cgccgcctcg cggtggcgca gcagcgtctc
     7741 gtacagcagc cgtgcggcgg cggcgccccg ctcggcctgg cgccatgcgg tgccggcgcg
     7801 ctccaggcgc gtctcggccg ccgcgaggcg gtcgtgcaag cccacttcgc cgagcgcgga
     7861 caggcggccc cgcagggtgg tgagctcgtc ctcccgctcg cgcagctcgc gccgctgctt
     7921 ctcggccgcc tcgcgcgcgt tgtcgacctc ggcctgcagg gtggccggat cctcgccctc
     7981 caggctcgcg cgggccgccg ccgcggcctg cgccgcctcg gccgcctcgg cggcgagcgc
     8041 ggccgcgcgc ttgcccaggc cggcgtccgc cgccgcaccc cgcgcggcgg ccagcgccgc
     8101 ctcggcggcc tcccgggcgg ccagcgcctg ctcggcgcgc agcgccagcc cggtgtgccg
     8161 ctcgcgcagc gccgcccgcc gcgccgaggt ctcccgcagc gcggactcgg catgctccag
     8221 cgcgtcctgg agctcgccga cccggcgctc ggcgtgctcg cgggcgcccc gcgccgcatc
     8281 gacatccgcg ccggcgtccg cggcggccag ccgctcgcgc aggcgcaccg ccttgccgtg
     8341 cagctcgtcg ggggacaggt cgcgcaggtt gtcgcgcagc acccggtcgg cctccgccag
     8401 cgcgcgctcc gcgtcgtcgc gccggcgcag cgccccacgc gcctcggcca ggtcggcgac
     8461 gcccgcgcgc gcgcaggcct cgcgccaggc gcgctccgcc tccgcgaggc ggtcccgcaa
     8521 ggcgctcagc ccctgcccgg gggacagcac ggcgtcgagc ccgcccacgc gcagccgcac
     8581 ctcctcggtg accggcagct cgtggcgcgc tccggcggcg aggtccagcg cctgcccgtc
     8641 gagctccacg gcgacgtcct ggcgcgcctc cagccgcagc agcgggccgg cgtcggcaag
     8701 gcgcgcctgc gcctgcgcca gcgcgagctg ggcgcgctcg atctccgccg cggctccctc
     8761 gtcgaccctg gcgtcctcca gcacctcgcg cgcctgcgcc gcctgggcgc gggcctgctc
     8821 cacgcgcgcc cagcgctcct ccagcagctc caggctgaac gcctccttgc tgcgcgcctc
     8881 ggcgtcgagg gcggcgcgga ccgcgtcgcg cgccgccgac agctccgcgc gcagcccggt
     8941 gacctgctcc tggaggcgct gctcctccgc cgccgcggcc tccagggcgg gggcggacgc
     9001 gtcctgctcg cgcagcgctt cctcggcggc cgccgcggtc gcctcagccg cctcggtgag
     9061 ccgcgcgcgc tcggcgatct cccgcgcggc gccctgcgcc gccgcctcgg cgcgctcggc
     9121 gcgcagggcc agccgctcca cctcggcggc gcgctcgcgc accgccgtcc agcgtcgctc
     9181 cagatcctcc agcagcagcc ggcggcgctg cagctccggg cgcagcgcct ccagctcccc
     9241 ggcgatgcgc gcggcgtcct ccacgtcccg ctccaggcct gccagcgccc gccgcgcctc
     9301 cgcggcctcg gcctcggcgg cgtccagctc ggcgcgcagg cccttgaacc acgcgttgtc
     9361 ctggccggtc ggcgtgaagg agcgcaggta ctccgcctgc gcccggtcca gcagggtgtg
     9421 ctcggcctcg ccgccgcgcg cgccggccgc ggcgacgtcc agcgcggcgg ccagcgcgct
     9481 cacctcggac aggtcgggct gctccaccgc ctggccctgg accatgcgca gcgcccgcca
     9541 cagcggcagg tcgaccgtct cggccaggat cgcgcgcacg cgttcgtgcg cctcccgccc
     9601 ggtgaactgc tccgggcggg gttcgtggac ggtgagcgtg gtgaagcgct cgcggtggaa
     9661 gcgcttggcg taggtgaacc ggtaggggcc ggcggacagc tccgcctcca cctccgtgcc
     9721 gacgtcggcg ccgaccggct tggtggcgcg cacgccggcc ttgcgggagt cgtcgtagtg
     9781 ctccagcagc aggtccagcg cctcggcgag gctggacttg cccgcctcgt tgggcccctg
     9841 caccaccgtg acgccacgct cggcgaaggc gacctcgcgc gcggcgacgc cgcggtagtt
     9901 cgccaggcgc agccggtgga tcctcacgcg gcggcgcccc ggtgcagccg gtacagcagg
     9961 gccagcgcgt cctgggccgc gtcggcgccg ccctcggcgg cggcgcgcag gtccccgacg
    10021 gcgtcggcgg cgaagcccgc cagctccagc tccgccaggt cgtcctcctc cggcagcagc
    10081 gccaggtcgc tgtggcgctc ccacacgcgc agggacgcga acagctcgcg gtggcggtcc
    10141 agcacgtcgt caagcagcgc catgtcgcgc agccccagcg agcccaccag ccgcaggcgc
    10201 acggcgcagc gctccttgtc gggctgctcg gccagccagg tcgccagcac gctgacgtcg
    10261 tcggcgtcgc gcagggcgaa cggttccggc tgggcgaagc gccacgtcgc cacggggtgc
    10321 gcctcggccc ggcagtcgcg cgcgtcgacg tccacgacca gcacgtggcc cggtgcgacc
    10381 tcgtcgaagt cggtgggctc cggcgcgccg gcgtagcgca cgcggccgcc ggcgccgagc
    10441 tccgtgagcg agtggcggtc tcccagcgcc acgtagtgca cgcggccgtc ggccagcgcc
    10501 cgctccagcg gcgccaggcc gatcacggcg gggtcgtcgg cgtccgggga cagcacgtcc
    10561 acgcagccgt ggccggcgac cacgcgcacc gtgccgtcgg cgcgcaggtc ctcgcaggcg
    10621 gcggcgacca ggtcgcgcag cggccgcttg gacgtccacg gcgcggtgac cagctccaca
    10681 ccgggcgcga ccggccacgc ccccgcgcgg tccagcacgg tgacgttcgc gggcctggcg
    10741 cgggcgaact ccgggctggt gaacagcgac ccggcgtcca gcgggtcgtg gttgccgggc
    10801 agcaggtaga cgggcaggcc caggccgccc atcacgtcca gggcgcgcag cagcacctgg
    10861 cggtccaggt ggttggactc gaagacgtcg ccgcacacca ggacgaacgc gcagccctcg
    10921 cgctgcgcga cgcgcccgat ctcgcgcacc gcctccagcc gcgcggcgcc gaagcgggcc
    10981 tgggcctcgt cggtcaggaa cgcccgcgtc atgccgagct gccagtcgcc cgtgtgcagg
    11041 aagcgcacca cgtggatccc tccgtgtccg tcgaattaca ccagtgtaag gagggggtgc
    11101 gacggcgtcg cgtgaaagtt cggtcagtcc tccaggacgc gcgccgcctc ggggtccagc
    11161 tcgcgcagga acaccgcgca gcggtccgcc tcctcgtgct cgccgatggc ccaggccgcc
    11221 cgccccagcg cgtgcacgct gcgcaggaag ccgcggttgg cctcgtgcga ccacggcacc
    11281 gggccgctgc cgcgccagcc cgcgcggcgc accttgtcca ggccgcggtg gtagccggtg
    11341 cgggcgaacg cgtacgccgc cacggggtcg ccggcgtgca gggcgcgctc ggccaggatc
    11401 gcccaggcct ccaggaaggt ggggtagctg gccgcgacct cccgcacggc gatgtccggc
    11461 acgtcgccgc cgagggcctg cgcgagcgcg tgccgcgcct cctgcggctc ctccggcagc
    11521 agcgtcggct ccggggcgtc ctccccggcg atgcgcaggc tcatgccgtg ctccccggga
    11581 cgaccatgtc gaaccagcgg tgcgcggcgc ggctggtgtc cgacgcggcg tggacgtgct
    11641 tggcctccgt ccactcgtgc aggccgagca gtccgtgctc ccgcccgatc ccggactggc
    11701 cgtagccccc gaacgggtag tgcgggttga gcaggtgcca ctcgttgacc cagacggtgc
    11761 cggcgcgcag ccggccggcc agcgccgtgg cgcgcgccgg cgagccccac acaccggcgg
    11821 ccaggccgta cgccgtgccg ttggcgatcg ccaccgcctc gtcctcgtcg cggtagcgca
    11881 gcacgctcag caccgggccg aagatctcct cctgggcgat ggtcatgtcg gcgcgcacgt
    11941 cggtgaacac ggtcggctcc acgaagaagc cttgcgccag ctcgccgtcg gtcaggcggc
    12001 tgccgcccag ggccagcgtc gcgccctcct tgccgccgat gtcgatgtag tgcagcaccg
    12061 tgtcgagctg cttctggctg accagcgggc cgagctggtt ggaggggtcg gccgtgggtc
    12121 cgacgcggat cgcgctggcg cgctcgagca ggcgccccac gaactcgtcg tgcagcgcgt
    12181 cgggcagcag cagcctgctg ccggctgtgc acgcctggcc cgagtgcagg aacaccgcgt
    12241 acagcgcgcc gtccaccgcc aggtccaggt cggcgtcgtc cagcacgatc tgcgcgctct
    12301 tgccgcccag ctccagggtg accttcttca gcgtgggcga ggccatgcgc atgatctccc
    12361 ggccgacgtt ggtggagccg gtgaaggcca ccttgtcgac gtccgggttg ctggccagct
    12421 cctcgccggt gcccgggccc gggccgggga tcacgttgac cacgccgtcc ggcaccccgg
    12481 cctcgcgcag gatctccgcc agccgcaccg ccgtgcacgg cgtctcctcc gcgggcttga
    12541 gcaccaccgt gttgccggcg gccagcgccg gggcgagctt ccatgccgcc atctgcagcg
    12601 ggaagttcca cggcacgatc gcgctgcaca cgccgaccgg gtcgcgccgc agcgtgttgg
    12661 ccgacccgaa cggcgagggc ggcagcgtct ccggctcgtc gagcgtggcg gccatgtcgg
    12721 cgcacgcgga gaaccactgg atcgccccgc ccacgtcggc ggccatggcc ttgaccagcg
    12781 tggagcccga gtcgcgccgc tccagctctg cgagctcacc ggcctgcgcg gcgatgaggt
    12841 cggcggcggc gcgcagcacg gcgccgcgct cggcgccggt cctgcccgac cagcgcccgt
    12901 cgtcgaaggc ggcgcgcgcg gcggcgatgg cggcctgcgc gtcctcgcgc cccgcgcgcg
    12961 ccaccgtgcc gagcgactcg ccggtggacg ggtccagcgt ctggaacgtc tcgcccgacg
    13021 cggcgtcgcg gaactcgccg tcgatgaaca gccggtagtc ggccatgggg cctctccctt
    13081 gcgcgtgtgc gactggtctt acttcatgac ttcgaacgag ttgcggcaat cctcgcagaa
    13141 gcgcagtgcc cggcacgggg tcgggccgaa cgccgactcc tggtcggtcc gcgcgctgtc
    13201 gcagtgcggg cagccggcgt ccatggagac gccgaaggcc ttcagcgccg tgcggccttc
    13261 ggcgctgatg cgcgccgggg accacacggg tgcggtgacc cagcgcacgc gcgcgtcggc
    13321 cacgcccggc atggcgcgca gcgccgcttc gacttcgtcg cggatcacct gcgtggccgg
    13381 gcagccggtg aacgtgggca ccaggtccac ctccacggcg gcgccctcca cgcgcaccgc
    13441 ggcgacgatg cccaggtcca gcacgctcag cgcgggcagc tcggggtcgg gcacgccggc
    13501 cagcgcggcg cgcacggcat ccggcgtggg ggcgctcacc acgccgctcc cgggtgcgcc
    13561 cggtgcaggc cggtgagctc ctcccacagc ggcgcgaact cgggcgtgcg cgcggtgcga
    13621 tcacccggct cggcgtccag caggccgccc agctcgccga ggccccagcc gtccaggtcg
    13681 gcgcggcgcc gcgcgcgcca cgccgccagc acggccgccg acgcacaggg cagcgtgccg
    13741 tcggcgacga gggtgtcctc gccgacgctg ggcgcgaaca ggcccggcgc ctcaggcagc
    13801 gcgcgctcca gcgcggcgcg caggcgggcg acggcctccc cgccggcggc ggccagccgc
    13861 cccacccact cctcggcgtg gcgcacgtgc caggcctgct cgcgcagcgg cgcggcggcg
    13921 gcgccggcca gttccggcca gccggacgcg gccagccact ccaggcgcgc ggtctcggcg
    13981 gtgtcggcca gccagtgccg cgccaccgtg tacgcccagt cgccgttggg ccgctccacc
    14041 agcgcgcagc tgcggtagtc ctccggcgcg cggcccagcg ccaggatgtc ggggtcgcgg
    14101 ccgtcggcgt cggcggccag g
//
LOCUS       DATMKC010000047         5294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_165519, whole genome shotgun sequence.
ACCESSION   DATMKC010000047 DATMKC010000000
VERSION     DATMKC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165519"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VNU01_01305"
     CDS             <1..266
                     /locus_tag="VNU01_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31287.1"
                     /translation="HVLRAARGLTAGEVGGRWVVEPSRGDAIRLALEAAEPGDVVVVA
                     GKGHEATQEFADRTVAFDDRLVAEAILRELSGEPGQPREGAGS"
     gene            263..1651
                     /gene="murF"
                     /locus_tag="VNU01_01310"
     CDS             263..1651
                     /gene="murF"
                     /locus_tag="VNU01_01310"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWH31288.1"
                     /translation="MIPLTLEQLAEVVGGRLQDGADAARVVGDVVIDSRRATAGALFV
                     ALPGEHADGHDYVADAVARGAAGYLADERRAPLPEPGAVLVDDPGDALLGLGAWVRDQ
                     VDPRVVAVTGSNGKTTTKDLIAAAVGAGLRTVANEGSYNNELGVPLTCCKLDEDSQVL
                     VTEIGARGIGHIASLTPLVRPDVAVVTTVAGAHLELFKDLDTVARAKGELVESLGPDG
                     VAVLNADDARVAAMRDRAMGRVVTYGVSADADWRAGDLRADELARFSFTARGVRVRCP
                     LPGEHNVGNALAALAVADVLGVAPETAAAGIGAARVSRWRMEVRRTPSGAVILNDAYN
                     ANPASMEAALKTLASMRVAGRRWAVLGRMAELGDGEREAHDRIGRLCIRLGVDGLVVV
                     GAQARAIRDAADLEGFYGQGDLFLVDAPAEAAELLRDRLAEGDAVLVKASRSAGLERV
                     ADLLAPQGEDAP"
     gene            1648..2682
                     /gene="mraY"
                     /locus_tag="VNU01_01315"
     CDS             1648..2682
                     /gene="mraY"
                     /locus_tag="VNU01_01315"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWH31289.1"
                     /translation="MRAILVAASVALLVSLVGTPLVIRFFRRRGYGQLIRDDGPQAHL
                     AKRGTPTMGGTAIIIAALVGYFTATLIFGPERSAGGLLAMGVFTGMGVVGFLDDYIKL
                     RRNRSLGLTKTAKFTGQALIAVVFAIGAQYVAETSTHLSFIGATELDFGMFFVLFCFL
                     ILSATSNGVNLTDGLDGLAAGTSSLVFGAYTVIAFWIFRHEADYSFEAYLHADALAIA
                     AAAAMGATLGFLWWNAPPAKIFMGDTGSLAIGGLLAALAVLTETELLLVILGGLYVVE
                     TSSVIIQVASFRLFGRRVFKMAPLHHHFELAGWQETTVIVRFWIIAGLSVAFALGLFY
                     ADFLARGGVG"
     gene            2747..4138
                     /gene="murD"
                     /locus_tag="VNU01_01320"
     CDS             2747..4138
                     /gene="murD"
                     /locus_tag="VNU01_01320"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWH31290.1"
                     /translation="MTLPRSAVVVGIGASGVAAARALASRGVHTVACDDRGGTDLERR
                     AAELVAAGVAFVAPGPQGSDHSLLQSYAGRAELFVASPGVPQSHPLLAAALDRGVPVW
                     SEPELAWRLSEGRTRVVAVTGTNGKTTTTELVAECLGVPAVGNIGTPMVQVLAAPDPP
                     PLVVAELSSFQLRFADTLRPDVAVLLNVAPDHLDWHGTVDAYRAAKARIWAAQQAGDR
                     VVAGADDDGARATVEAHPPPGRLAWFRSGAPADGEVGVADGWITQRLRAGAPERVAEV
                     ARMALRGPHNVANACAAVAAALLAGGDPERIAATVAAFHPGPHRMEPVASAGGVDWVN
                     DSKATNPHAAAAALSAYPSVVWIAGGLTKGLRFDELRPLLPGRVRVALTIGSGGPEIA
                     ALVRGCGVEAIEAGTLDEAVARAAGLARPGDTVLLSPACASMDQFRDYAERGQRFREL
                     AAAAASVSEEVER"
     gene            4135..>5294
                     /gene="ftsW"
                     /locus_tag="VNU01_01325"
     CDS             4135..>5294
                     /gene="ftsW"
                     /locus_tag="VNU01_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWH31291.1"
                     /translation="MSTTRRPAPRRRPPASAVLAARWRLLTAAGRTSPEALLLAGTVL
                     VLTVLGLVMTFSASFVASAAETGGAFDIFRRQLLWCLLGAPLLFGAARTDYRVWRPLA
                     APLLAVSLLAAALVLIPGVGVMVNGARRWIDLGPMRFQPTELLKLSVPLYLAHVLAAR
                     WPRLRDGDLRALVLPALPLLAVTGLLVMLEPDLETAALIVVIGAIPMYLAGLPGRLMA
                     AGLGAGAVLGTAGILMEDFRRQRFAAWLNPMGHADTFGFQSVQGFIALGNGGWAGVGL
                     GQGRGKWLYIPNAHTDFIFAIISEELGLLGALFVLTLFLALAVAGVRTARLAADPFGR
                     LLAGAITGWLVLQAGMNMGSVVGLLPVTGVTLPMVSFGGSSLVFTMLGLGIL"
BASE COUNT          576 a         1987 c         1976 g          755 t
ORIGIN      
        1 agcacgtcct gcgcgcggcg cgcggcctca ccgccggcga ggtcggcggg cgctgggtgg
       61 tcgagccgtc gcgcggcgac gccatccgcc tggcgctgga ggcagccgag cccggcgacg
      121 tcgtcgtggt cgcgggcaag ggccacgagg ccacgcagga gttcgccgac cgcaccgtcg
      181 ccttcgacga ccggctggtc gccgaggcca tcctgcgcga gctgtccggc gagcccggcc
      241 agccgcgtga aggagccggc tcgtgatccc cctgacgctc gagcagctcg ccgaggtggt
      301 cggcggacgg ctccaggacg gcgccgacgc ggcgcgcgtg gtcggcgacg tggtgatcga
      361 ctcgcgccgc gccaccgccg gcgcgctgtt cgtcgcgctg ccgggcgagc acgccgacgg
      421 ccacgactac gtcgccgacg cggtcgcgcg cggcgcggcc ggctacctgg ccgacgagcg
      481 gcgcgccccg ctccccgagc ccggggcggt gctcgtcgac gaccccggcg acgccctgct
      541 ggggctgggc gcgtgggtac gcgaccaggt cgacccgcgc gtcgtcgccg tcaccggctc
      601 caacggcaag accaccacca aggacctgat cgcggcggcc gtcggggcgg ggctgcgcac
      661 cgtcgccaac gagggctcct acaacaacga gctgggcgtg ccgctgacgt gctgcaagct
      721 ggacgaggac agccaggtgc tggtgaccga gatcggcgcg cggggcatcg gccacatcgc
      781 gtcgctgacg ccgctggtgc gccccgacgt cgccgtggtc accacggtcg ccggcgcgca
      841 cctggagctg ttcaaggacc tcgacaccgt ggcgcgcgcc aagggcgagc tggtggagtc
      901 gctgggcccc gacggcgtcg cggtgctcaa cgccgacgac gccagggtcg cggccatgcg
      961 cgaccgcgcg atggggcgcg tggtgaccta cggcgtgtcc gccgacgccg actggcgcgc
     1021 cggggacctg cgggccgacg agctcgcgcg cttctccttc acggcgcgcg gcgtgcgcgt
     1081 gcgctgcccg ctgccgggcg agcacaacgt cggcaacgcg ctggccgcgc tggccgtggc
     1141 cgacgtgctc ggcgtggcgc cggagacggc cgccgccggc atcggggcgg cgcgcgtgtc
     1201 gcgatggcgc atggaggtgc gccgcacgcc gtcgggcgcc gtcatcctca acgacgccta
     1261 caacgccaac cccgcctcca tggaggccgc cctgaagacg ctggcgtcca tgcgcgtggc
     1321 cgggcgccgc tgggcggtgc tggggcgcat ggccgagctc ggcgacggcg agcgggaggc
     1381 gcacgaccgc atcggccggc tgtgcatccg gcttggcgtg gacggcctgg tggtcgtcgg
     1441 cgcgcaggcg cgcgccatcc gcgacgccgc cgacctcgag ggcttctacg ggcagggcga
     1501 cctgttcctg gtggacgcgc ccgccgaggc cgcagagctg ctgcgcgacc ggctcgccga
     1561 gggcgacgcg gtgctggtga aggccagccg ctcggccggc ctggaacgcg tcgcggacct
     1621 gctcgcgccg caaggggagg acgccccgtg agggccatcc tggtggccgc gagcgtggcg
     1681 ctgctggtgt ccctggtggg caccccgttg gtgatccgct tcttccgccg ccgcgggtac
     1741 gggcagctca tccgcgacga cgggccgcag gcgcacctgg ccaagcgcgg cacccccacc
     1801 atgggcggca ccgcgatcat catcgccgcg ctggtcggct acttcaccgc caccctgatc
     1861 ttcggcccgg aacgctccgc cggcgggctg ctggcgatgg gcgtgttcac cggcatgggc
     1921 gtcgtcggct tcctggacga ctacatcaag ctgcggcgca accgcagcct ggggctgacc
     1981 aagaccgcca agttcaccgg gcaggcgctc atcgcggtgg tgttcgccat cggcgcccag
     2041 tacgtcgcgg agacctccac gcacctgtcg ttcatcggcg cgacggagct ggacttcggg
     2101 atgttcttcg tcctgttctg cttcctgatc ctgtcggcca cgtccaacgg cgtgaacctc
     2161 accgacgggc tggacggcct cgccgccggg accagcagcc tggtgttcgg cgcctacacc
     2221 gtgatcgcgt tctggatctt ccgccacgag gcggactact ccttcgaggc gtacctgcac
     2281 gccgacgcgc tcgccatcgc cgcagccgcg gcgatgggcg ccacgctggg cttcctgtgg
     2341 tggaacgcgc ccccggccaa gatcttcatg ggcgacaccg gctcgctggc catcggcggc
     2401 ctgctcgccg ccctggccgt gctcaccgag acggagctgc tgctggtcat cctgggcggg
     2461 ctgtacgtgg tggagacctc cagcgtgatc atccaggtcg cgagcttccg cctgttcggc
     2521 aggcgcgtgt tcaagatggc gccgctgcac caccacttcg agctggcggg ctggcaggag
     2581 accacggtga tcgtgcgctt ctggatcatc gcgggactgt ccgtcgcgtt cgccctcggc
     2641 ctgttctacg ccgacttcct cgctcgcggg ggcgtcggat gacgccccgc tgcgctcgtc
     2701 agggggccga cttcccattc acgcccgttg gtcgtggagg catcgagtga cgctgccccg
     2761 gtcggcggtg gtcgtcggca tcggtgcgtc gggcgtcgcg gccgcaaggg cgctcgcctc
     2821 acgtggcgtg cacaccgtcg cctgcgacga ccggggcggc acggaccttg agcggcgcgc
     2881 cgccgagctc gtcgccgcag gagtggcgtt cgtggccccg gggccccagg ggtcggacca
     2941 ttcgctgttg cagtcctatg ccgggcgggc ggagctcttc gtcgcgagcc ctggtgtgcc
     3001 gcagtcgcat ccccttctcg cggcggcgct cgatcgtggc gtgccggtct ggagcgagcc
     3061 ggagctggcg tggcggctgt ccgagggccg cacgcgcgtg gtcgccgtca ccgggaccaa
     3121 cggcaagacc accaccaccg agctcgtcgc cgagtgcctc ggcgtccccg ccgtcggcaa
     3181 catcggcacg cccatggtcc aggtcctggc cgcgccggac cccccgccgc tggtggtcgc
     3241 ggagctgtcc agcttccagc ttcgcttcgc cgacacgctg cgccccgacg tcgcggtgct
     3301 gctgaacgtc gcgcccgacc acctcgactg gcacgggacg gtggacgcct accgggcggc
     3361 gaaggcccgc atctgggcgg cgcagcaggc aggggaccgg gtcgtcgccg gcgcggacga
     3421 cgacggcgcg cgcgccaccg tggaggcgca cccgccgccc ggccgcctcg cgtggttccg
     3481 ctccggcgcc cccgccgacg gggaggtcgg cgtcgcggac ggctggatca cccagcggct
     3541 gcgcgccggc gcgcccgagc gcgtcgccga ggtggcgcgc atggcgctgc gcggcccgca
     3601 caacgtggcc aacgcctgcg ccgccgtcgc cgccgcgctt ttggccggcg gcgaccccga
     3661 gcgcatcgcc gccaccgtcg ccgcgttcca tcccgggccc caccgcatgg agccggtcgc
     3721 gtccgccggc ggggtggact gggtcaacga ctccaaggcc accaacccgc acgcggccgc
     3781 cgccgccctg tccgcctacc cgtccgtcgt ctggatcgcg ggcgggctga ccaagggcct
     3841 gcgcttcgac gagctgcggc cgctgctgcc cggacgggtc cgggtggcgc tcacgatcgg
     3901 ctccggcggg ccggagatcg ccgcgctcgt ccgcggttgc ggggtggaag cgatcgaagc
     3961 gggcaccctg gacgaggccg tggcccgggc cgccggcctg gcgcgccccg gcgacacggt
     4021 gctgctgtcg ccggcgtgcg cgtccatgga ccagttccgc gactacgccg agcgggggca
     4081 gcggttccgc gaactggccg cggcggcggc gagcgtgagc gaggaggtgg agcggtgagt
     4141 acgaccaggc gtcccgcgcc gcggcgccgc ccgcccgcgt ccgccgtgct ggccgcccgc
     4201 tggcgcctgc tcaccgcggc cgggcgcacg tcgcccgagg cgctgctgct ggccggcacc
     4261 gtcctggtcc tgaccgtgct gggactggtg atgacgttca gcgcctcctt cgtggcgtcc
     4321 gccgcggaga ccggcggcgc gttcgacatc ttccgccgcc agctgctgtg gtgcctgctc
     4381 ggcgcgccgc tgctgttcgg cgcggcgcgc accgactacc gcgtgtggcg gccgctggcc
     4441 gcgccgctgc tcgccgtgtc gctgctggcc gcggcgctcg tgctgatccc cggcgtcggc
     4501 gtgatggtca acggcgcgcg ccgctggatc gacctggggc ccatgcgctt ccagcccacc
     4561 gagctgctga agctgtccgt gccgctgtac ctcgcgcacg tgctggccgc gcgctggccg
     4621 cgcctgcgcg acggcgacct gcgcgccctg gtgctgcccg cgctgccgct gctcgcggtg
     4681 accggcctgc tggtgatgct ggagccggac ctggagaccg ccgcgctgat cgtcgtcatc
     4741 ggcgccatcc cgatgtacct cgcgggcctg cccggccgcc tcatggccgc cggcctcggc
     4801 gcgggcgccg tgctcggcac cgccggcatc ctcatggagg acttccggcg gcagcgcttc
     4861 gccgcgtggc tcaacccgat gggccacgcg gacacgttcg gcttccagtc cgtgcagggc
     4921 ttcatcgcgc tgggcaacgg cggctgggcg ggcgtgggcc tgggccaggg ccgcggcaag
     4981 tggctgtaca tccccaacgc ccacaccgac ttcatcttcg cgatcatcag cgaggagctc
     5041 ggcctgctcg gcgccctgtt cgtgctcacg ctgttcctgg cgctggccgt ggccggcgtg
     5101 cgcaccgccc ggctggccgc cgacccgttc gggcgcctgc tcgccggcgc gatcaccggc
     5161 tggctggtgc tgcaggccgg catgaacatg ggctcggtgg tgggcctgct gcccgtgacc
     5221 ggcgtcacgc tgccgatggt gagcttcggc ggctcctcgc tggtattcac catgctgggc
     5281 ctcggcatcc tgat
//
LOCUS       DATMKC010000048         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_232383, whole genome shotgun sequence.
ACCESSION   DATMKC010000048 DATMKC010000000
VERSION     DATMKC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232383"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VNU01_01330"
     CDS             <1..1615
                     /locus_tag="VNU01_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HWH31292.1"
                     /translation="NEIKQLVNQQRTDREDYVTRAGRYLQQELEAVGIQAEISGRAKH
                     FYSIYVKMTKKGREFNEIYDLTAMRVLVDSVKDCYGAIGIIHSLWKPLPGRFKDFVAM
                     PKFNMYQALHTTVIGPEGRPLEIQIRTREMHATAEYGVAAHWIYKGDGYTKGTGSAPK
                     AVEQKVEWLRHLLDWQEDLTDPQEFAENLKADLFEDEVFVFTPKGEVKSLSAGATPLD
                     FAYSVHTDVGHRCVGAKVNGKIVPLHYELESGDIVEVLTSKKERGPSRDWLSLVKTPR
                     AQSKIRAWFKRERREDSERTGRDILQENLKRQGLPAQKIVGSPLLADVIREMGFKKGE
                     DFYVALGAAKVSAKVVVNKVMQRLKQGESAVEPTGADELLQRKEPGARKTKASSAYGI
                     KVQGIDDVMLRLAKCCRPVPGDEIVGYISLGKGITIHREDCPNAQALMKNPERFTPVY
                     WEGDTAAAFRVELQVDGWDRTGLLEDLSRTFAETGINILEARCTVMHPMVKNRFVVEV
                     GDTASLKNCVQRLRNIESVFDAYRVTPGV"
     gene            1631..2050
                     /locus_tag="VNU01_01335"
     CDS             1631..2050
                     /locus_tag="VNU01_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWH31293.1"
                     /translation="MSHEDVEIVRRATGAFNRGDFGLVAELCDDDLEFVSVMTAVQET
                     TYRGRDTWERYAADMRETWAEWRLEDLRLLDAGDHTVTVLMRLVGAGKRSGVPVDREI
                     GVVYRLRDGRLWRVRSYPDARLALEAAGLSEQRARGS"
     gene            complement(2047..2436)
                     /locus_tag="VNU01_01340"
     CDS             complement(2047..2436)
                     /locus_tag="VNU01_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31294.1"
                     /translation="MRPPRVRLETDAGTVEGVTVIVQNSDPFTFYGRRPLRVCEDAGL
                     ETGSISLTVLKRATVLEMPTVVPRLFSSSAQRVQRHRQIEGFTGVSHARVTSLAGTFP
                     VQVDGDYIGEFEQVTYGVAPRSLAVVS"
BASE COUNT          445 a          861 c          882 g          367 t
ORIGIN      
        1 caacgagatc aagcagctgg tcaaccagca gcgcaccgac cgcgaggact acgtcacccg
       61 agcgggccgc tacctgcagc aggagctcga ggccgtcggc atccaggccg agatctcagg
      121 ccgcgccaag cacttctact ccatctacgt gaagatgacc aagaaggggc gcgagttcaa
      181 cgagatctac gacctcaccg ccatgcgcgt gctcgtggac tcggtcaagg actgctacgg
      241 cgccatcggg atcatccact cgctgtggaa gccgctgccg ggccgcttca aggacttcgt
      301 ggcgatgccc aagttcaaca tgtaccaggc gctccacacc acggtcatcg ggccggaggg
      361 tcgcccgctg gagatccaga tccgcacgcg cgagatgcac gccacggccg agtacggggt
      421 cgccgcgcac tggatctaca agggcgacgg ctacaccaag ggcaccggct ccgcgcccaa
      481 ggcggtcgag cagaaggtcg agtggcttcg ccacctgctc gactggcagg aggacctcac
      541 cgatccgcag gagttcgccg agaatctcaa ggccgacctc ttcgaggacg aggtcttcgt
      601 gttcacgccc aagggcgagg tcaagtcgct gtcggccggc gccaccccgc tggacttcgc
      661 gtactcggtc cacaccgacg tcgggcaccg ctgcgtgggc gccaaggtca acggcaagat
      721 cgtgccgctc cactacgagc tcgagtccgg cgacatcgtc gaggtgctca cctccaagaa
      781 ggagcggggg ccgtcgcgcg actggctcag cctcgtcaag acgccacgcg cccagtccaa
      841 gatccgggcc tggttcaagc gcgagcgccg cgaggactcc gagcgcaccg gtcgcgacat
      901 cctccaggag aacctcaagc gccagggcct gccggcacag aagatcgtcg ggtcgccgct
      961 gctcgccgac gtcatccgcg agatgggctt caagaagggc gaggacttct acgtggcgct
     1021 gggcgcggcc aaggtctcgg ccaaggtcgt cgtcaacaag gtcatgcagc ggctcaagca
     1081 gggcgagtcc gccgtggagc ccaccggcgc cgacgagctg ctccagcgca aggagcccgg
     1141 cgcgcgcaag accaaggcct cctccgccta cggcatcaag gtccagggca tcgacgacgt
     1201 gatgctgcgc ctggccaagt gctgccggcc ggtgcccggc gacgagatcg tgggctacat
     1261 ctcgctcggc aagggcatca ccatccaccg cgaggactgc cccaacgccc aggcgctcat
     1321 gaagaacccc gagcggttca cgcccgtgta ctgggagggc gacaccgcgg ccgccttccg
     1381 cgtcgagctc caggtcgacg gatgggaccg caccgggctg ctcgaggacc tgtcgcgcac
     1441 cttcgcggag accggcatca acatcctcga ggcgcgctgc acggtcatgc accccatggt
     1501 caagaaccgc ttcgtggtgg aggtggggga caccgcctcg ctcaagaact gcgtgcagcg
     1561 gctgcgcaac atcgagagcg tcttcgacgc ctaccgcgtc acgcccggcg tctgagcgtc
     1621 accgcgggcg atgtcacacg aggacgtcga gatcgtgcgc cgtgcgactg gagcgttcaa
     1681 tcgcggtgac tttggcctgg tggctgagct ctgcgacgac gatctcgagt tcgtgtccgt
     1741 gatgacggcg gttcaggaga ccacgtaccg agggcgtgac acttgggagc gctacgccgc
     1801 ggacatgcgc gaaacgtggg cggagtggag gttggaggac ctccgccttc tcgatgccgg
     1861 cgatcacacc gtcacggtgc tcatgcgcct ggtcggcgcc gggaagcgaa gcggcgtccc
     1921 ggtggaccgc gagatcggcg tggtgtaccg cctccgggac ggaaggctct ggagggtccg
     1981 ttcgtacccc gacgccaggc tcgccctcga agccgccggg ctgtcggaac agcgcgctcg
     2041 agggagctag ctgaccacgg ccagcgagcg cggggccacc ccgtaggtga cctgctcgaa
     2101 ctcgccgatg tagtcgccgt cgacctgcac cgggaaggtg ccggcgaggc tggtcacgcg
     2161 ggcgtggctg acgcccgtga atccctcgat ctggcggtgg cgctgcacgc gctgggcgct
     2221 ggaggagaac agtcgcggga cgacggtcgg catctccagc acggtggcgc gcttgagcac
     2281 ggtcagcgag atcgagccgg tctccaggcc ggcgtcctcg cacacgcgga gcgggcggcg
     2341 gccgtagaag gtgaacgggt cggagttctg cacgatcacc gtgaccccct cgaccgtgcc
     2401 ggcgtcggtc tccaaccgga cgcgcggcgg gcgcacgagg tacttgcgcg tgaacgtggt
     2461 gaccgcgctc gacctctccc gcctggatgc gctgctcggc catccgtgag ttctagcgca
     2521 gcagcgaggt gggaggcttc ggcgcgcggg cgcgt
//
LOCUS       DATMKC010000049         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_89213, whole genome shotgun sequence.
ACCESSION   DATMKC010000049 DATMKC010000000
VERSION     DATMKC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89213"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VNU01_01345"
     CDS             complement(<1..1006)
                     /locus_tag="VNU01_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31295.1"
                     /translation="MPFAHTAPGTAATLLSFGSDWLYVLLTGGALLGIGALLALRGTS
                     SAARHPLRAYMQGAAAVLGLPRWSAGPILVLIAGCVYAVGGFFWDVGWHIAVGRDEFL
                     FSPPHLCLLIGIGLLGLAGLAATVAATRDRAGAGWGIGRVRVPYGAGALLATGVVAGV
                     GFGVDELWHWAYGLDVSMWSPPHLSMISAAAFSPMAGWLLLAEAGPGAGRKVPRYLLV
                     SLFTGATLVGLSAWQLEFDMGVPQWRQAFHPLLVGVAGAFALCAARAALGPGGAMLGM
                     ARFVLIRVGLALLTTQVWHLPEPRFVPYLAAALAVEAAFALTRRAAPPRAAVVAGVGV
                     A"
     gene            1121..1897
                     /locus_tag="VNU01_01350"
     CDS             1121..1897
                     /locus_tag="VNU01_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWH31296.1"
                     /translation="MDLRIDELALRTGVSSRNIRAYQQRGLLPPPRLRGRTGFYSEEH
                     LRRLVLIRDLQQRGFSLAAIQETLEAWSKGGDISDLLGFHEMLRQGWGEEPRVELTRE
                     QVVELFPEAAQREELVRRAVERGLLEPSEGGYRAPQVLIQAGADLVRAGIPVEEIFDL
                     VETIRASAAEIAGRFIELVAERLIEAITSGLASAEEIHEVTQSVLGLHAVAIDVVRPF
                     LAAELAAATEAALERFRQRGQPVAEAGAQGQASRGLHSRR"
     gene            complement(1880..2320)
                     /locus_tag="VNU01_01355"
     CDS             complement(1880..2320)
                     /locus_tag="VNU01_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWH31297.1"
                     /translation="MSTMLWPYLPDTTEPAMRPYWEGLDRGVLVLPRCGSCVRFVWYP
                     VHRCPDCLGELEWAEVSPDGSLFSFTVVQRAFADEWKDAVPLVICLVELSEAPGVRLV
                     CRAVQGEPSVPDIGSPLRIEFERLGDGVVMPVVRLAGAESPAAV"
     gene            complement(2317..2919)
                     /locus_tag="VNU01_01360"
     CDS             complement(2317..2919)
                     /locus_tag="VNU01_01360"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH31298.1"
                     /translation="MSREETIVAAATQLFAEYSYAAVGIRAIAESVGIMSSSLYYYFP
                     SKEDILFRICQQATRDFIAVHGAVLASHETGGSRAETLKTLMGSHVRYFWENRLAQQV
                     AARELSQLSPERFEEVRDWQRAYTADLVEYVEMGVAAGEFDVPEPAIVVRAALDMLNS
                     VFRWFTPRKNLGIDEVAELYADLIVDRLLGAAHVSRGENR"
     gene            complement(3086..>3674)
                     /locus_tag="VNU01_01365"
     CDS             complement(3086..>3674)
                     /locus_tag="VNU01_01365"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWH31299.1"
                     /translation="TGTEAGAVGAFAALVIAIIARRSSDKSLPQLLIASAREAIEVTS
                     MIFMLLIGGAVFTFFIVSAGLPITVTEWILGLPVDPNIVLALFLLAIIPLGMFLDGLS
                     ILLFTVPIMAPVVVQSGFDGVWFGVLVVKTIEMGLITPPVGINVFIISGLVPDLRVEH
                     VFRRVGPFVALDLAVTALFFLVPGIVTWLPRAAGL"
BASE COUNT          630 a         1321 c         1268 g          455 t
ORIGIN      
        1 tggccacgcc caccccggcc accacggcgg cgcgcggcgg cgcggcgcgg cgcgtcaggg
       61 cgaacgcggc ctccaccgcc agcgccgccg ccaggtaggg cacgaagcgt ggctcgggca
      121 ggtgccagac ctgcgtggtg agcagggcca ggcccacgcg gatcagcacg aaccgcgcca
      181 tgccgagcat cgccccgccg gggccgagcg cggcgcgcgc ggcgcacagg gcgaacgcgc
      241 cggccacgcc caccagcagc gggtggaacg cctggcgcca ctgcggcacg cccatgtcga
      301 actcgagctg ccaggcggac aggccgacca gggtggcccc ggtgaacagg ctcaccagca
      361 ggtagcgcgg gaccttgcgg ccggcgcccg ggccggcctc cgccagcagc agccagcccg
      421 ccatggggct gaacgccgcc gccgagatca tcgacaggtg cggcgggctc cacatgctga
      481 cgtccaggcc gtaggcccaa tgccacaact cgtccacgcc gaagccgacg ccggccacga
      541 ccccggtggc cagcagcgcc ccggcgccgt agggcacgcg cacgcggccg atgccccagc
      601 cggcgcccgc gcggtcgcgc gtggccgcga ccgtggccgc gagccccgcc agccccagca
      661 ggccgatgcc gatcagcagg cacaagtgcg gcgggctgaa caggaactcg tcacggccca
      721 cggcgatgtg ccagccgacg tcccagaaga acccgccgac ggcgtacacg cagccggcga
      781 tcaggaccag gatcggcccg gcgctccagc gcggcaggcc gagcaccgcg gccgccccct
      841 gcatgtacgc gcgcagcgga tggcgcgccg cggaggacgt gccccgaagg gccaacagcg
      901 cgccgatgcc gagcagggcc ccgccggtca gcagcacgta cagccagtcc gagccgaacg
      961 acagcagggt cgcggcggtt ccgggggccg tgtgagcgaa tggcatgcgt tgctccgtgc
     1021 ggtgttcgtg ggatgctgcg gcactccggc cggtcggcga cggttgcatg taatcatgtc
     1081 acattcatag gtgcgatatg cgttggccta ccattgcgcg gtggacctgc ggatcgacga
     1141 actggcgttg cgcacgggag tgagctctcg caacatccgc gcgtaccagc agcgcgggct
     1201 gctgcccccg ccgaggctgc gcggccgcac cgggttctac agcgaggagc acctgcgtcg
     1261 cctggtgctc atccgcgacc tgcagcagcg cggcttctcc ctcgccgcga tccaggaaac
     1321 cctggaagcg tggagcaagg gcggtgacat cagcgatctg cttggattcc acgagatgct
     1381 gcgccagggg tggggcgagg agccccgcgt cgagctgacc cgcgagcagg tggtggaact
     1441 gtttcccgag gccgcgcagc gcgaggagct ggtgcgccgc gcggtcgagc gcggcctgct
     1501 cgaaccctcc gaaggcgggt atcgcgcgcc gcaggtgctg atccaggccg gcgcggactt
     1561 ggtgcgcgcc ggcatcccgg tggaggagat cttcgacctg gtggagacga tccgggcgag
     1621 tgcggccgag atcgccggcc gcttcatcga gctcgttgcc gaacggctca tcgaggccat
     1681 cacctcaggc ctcgccagcg ccgaggagat ccacgaggtg acccagagcg tgctcgggct
     1741 ccacgccgtc gcgatcgacg tggtgcggcc gttcctggcg gccgaactcg ctgcggcgac
     1801 ggaggcagcc ctggaacgct tccgccagcg cggccaaccc gtagcggagg cgggtgctca
     1861 gggacaggct tcgcgcggct tacacagccg ccggtgactc cgcgccggcc agccgcacca
     1921 ccggcatgac gacaccatcg cccagccgct cgaattcgat ccggagaggg ctgccgatgt
     1981 ccggcaccga cggctcgccc tggaccgcgc ggcataccag gcgcacgcca ggggcctccg
     2041 acaactccac gaggcagatc acgagtggca cggcgtcttt ccattcgtcc gcgaacgcgc
     2101 gctgcaccac ggtgaaggag aacagcgaac cgtccggcga gacctcggcc cactccagct
     2161 cgcccaagca gtccgggcag cggtgcacgg ggtaccagac gaagcgaacg caggacccgc
     2221 accgcggcag aacgagcacg ccgcgatcca ggccctccca gtacggccgc atggccggtt
     2281 cggtcgtgtc cggcaggtag ggccacaaca tggtgctcat cggttctctc cgcgcgagac
     2341 gtgagccgct cccagcagcc ggtccacaat caggtcggcg tacagttccg cgacctcgtc
     2401 gatacccaga ttcttgcggg gcgtgaacca gcggaagacg gaattgagca tgtccagggc
     2461 cgcgcgcacc acgatggcgg gctcgggcac gtcgaactcc ccggcggcca cccccatctc
     2521 cacgtactcg acgaggtcgg cggtgtaggc gcgctgccaa tcccggactt cctcgaagcg
     2581 ctccggcgac agctggctca gctctcgggc ggcgacctgt tgcgccaacc gattctccca
     2641 gaagtagcgg acgtggctcc ccatcagtgt cttcagcgtc tccgcccggc tcccgccggt
     2701 ctcatgggag gccagcacgg ccccgtgcac ggcaatgaaa tccctggtcg cctgctggca
     2761 gatgcggaag agaatgtctt ctttggaagg aaagtagtag tacagcgagg aggacatgat
     2821 gccgacgctc tcggcgatcg cgcggatccc gacggcggcg tagctgtact cggcgaagag
     2881 ctgcgtggcc gccgcgacga tcgtctcctc gcggctcatc tccccggcct tgctcgctgt
     2941 cacttccata tcgccactcc cccgggcacc cagagctgca gaccggcggc ccgcgcggcg
     3001 gtccacgcgt cgcggagcat acccgtcgac ccgatgaggg ctcccgcgcg cgccctgctg
     3061 ccggcgtcta ccgccacgtc ggcgatcaca ggccggccgc ccgcggcagc cacgtgacga
     3121 tgccgggaac gaggaagaac aacgccgtca ccgccaggtc gagcgccacg aacggtccca
     3181 cgcggcggaa cacgtgttcc acgcgcaggt ccggcacgag gcccgagatg atgaacacgt
     3241 tgataccgac cggcggcgtg atgagcccca tctcgatggt cttgaccacc aggacgccga
     3301 accagacccc gtcgaagccg gactgcacga cgacgggcgc catgatcggc accgtgaaca
     3361 gcaggatcga caggccgtcc aggaacatcc ccaacgggat gatcgccaac aggaacaggg
     3421 ccagaacgat gttggggtcg accggcagtc cgaggatcca ctcggtcacg gtgatgggga
     3481 ggccggcgga cacgatgaag aacgtgaaga ccgcgccgcc gatgagcagc atgaagatca
     3541 tgctcgtgac ttcgatggcc tcgcgcgccg aggcgatcag caactgcggc agcgacttgt
     3601 cgctggagcg ccgcgcgatg atcgcgatca ccagcgcggc gaacgctccc accgcaccgg
     3661 cttccgtccc agtg
//
LOCUS       DATMKC010000050         6399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_185333, whole genome shotgun sequence.
ACCESSION   DATMKC010000050 DATMKC010000000
VERSION     DATMKC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185333"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /gene="ligD"
                     /locus_tag="VNU01_01370"
     CDS             <1..2249
                     /gene="ligD"
                     /locus_tag="VNU01_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWH31300.1"
                     /translation="TVDYLLADDARGLLWLANLGCIEMHPWHSRVDDIGHPDYAFFDL
                     DPMDVSFGTVREVALLVRTVLDQLGLRGYPRTSGATGIQVYVPLERVHSAARIRDWVG
                     RVCRLINRADPERTTMEWAVADRTGKVFLDHNMNTEGRNIAAAYSVRPERRATVSAPV
                     TWEELEGDVEPGDFTMASIWARLDEVGDLFAPVLAGGQDLRAAMEAVGMPAESADDEE
                     RGHDVGPVLDGGDESPAARLRRYQSERAGEEPAAARRLRTYAAKRDFSVTPEPRGVST
                     ESSSDGLSRHTPGEQRFVIQHHLATRLHHDLRFTRDGVAPSWALPKGLPDVPGVRHLA
                     VQTEDHPIDYMTFSGEIPEGEYGAGPVRIWDSGTYEALEWSQDKVSVRLHGGRHRGEW
                     HLFRIGGQDEPAVWNVIRSDTPDEAELPAEPAACEPMLATLTERPFDDPKWLFEVKWD
                     GVRAIATVMRPTQGQDGWTRLVSRLGNDVSAGYPELEGLWERVLARGAVLDGEIVAFD
                     ERGNPSFQRLQRRMHVRGAALERLRRQVPVTYVAFDLLWCDGESLLDRPLQERLARLE
                     DVLVPGTHAQRSEAIAEHGLALYDAARERGLEGVIAKRLDSRYQPGRRSRDWLKLKVR
                     RRVDCVIAGWLPGEGARARLGSLLLGMWEGDRLRYVGKVGTGFDQAELERLRALLDER
                     ATDERPFAKAAPNPPRPSKWARPELVCTVEYAEVTEGGQLRAPSYKGIVEGADPRACR
                     YEDLPLST"
     gene            complement(2222..3223)
                     /locus_tag="VNU01_01375"
     CDS             complement(2222..3223)
                     /locus_tag="VNU01_01375"
                     /inference="COORDINATES: protein motif:HMM:NF017810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HWH31301.1"
                     /translation="MATTAVLQLLLPVAHAAEPSTAFVARPADGSSTADGGWFLYDDV
                     APGAALSGELAVRNDSGGPLTLRIAAADAQTAQQGGVSYSLPGEELERVGRWLDLSRE
                     TVELAPGQGEVVPFTVAVPGDARPGDHLAGIAVWAPGAGDEPPAGEGPGAGVVIETRQ
                     VIAVQVTVPGSAAHELEVKAVRIAARPSGPFLEVTVANTGTLLTKAQARVLVDGEPAA
                     QALQVETIVPGTSFGLPVEWPFADLDDAHHVAVELDYEGGTARWEGTLDADDEVRAAL
                     EDWAAPGTGEPSPPVVVILLGAVVAALVILLSLVRWWRRRAGELGSSPAHVDSGRSS"
     gene            complement(3366..3938)
                     /locus_tag="VNU01_01380"
     CDS             complement(3366..3938)
                     /locus_tag="VNU01_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HWH31302.1"
                     /translation="MLKRKIAAVAATVALAGAIAAPAAASEVAVTGGSLAFTSAPVAG
                     DFSALTLDGTTKTTTAAVPAFEVNDSRGTGAGWNITVQGTQLKEWDGALNAGAGGYVT
                     GGKTIATSSLALSQPTVAADGTTSAAPSITAGPYTIDAGSAVKIASAAVDTGMGKYDF
                     GATTMTLTVPPNTYAKTYRSDVTVTLATTP"
     gene            complement(4357..4845)
                     /locus_tag="VNU01_01385"
     CDS             complement(4357..4845)
                     /locus_tag="VNU01_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31303.1"
                     /translation="MTDGVRERQEGDGFDPVLGEAPGAAVGRDRYRPVFAELQVFPGP
                     TSTEVDVTLSLGGRVVTSRAQGPGDERGVAHTAAEAALSALGELLDGRATLELAWFKV
                     LEPDGHFGHVVLHTAVACRSERGRDLYVGSALSRGDLAVAAVRSALDAMNRPFARLLA
                     EG"
     gene            4986..5345
                     /locus_tag="VNU01_01390"
     CDS             4986..5345
                     /locus_tag="VNU01_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH31304.1"
                     /translation="MRVLLVDDSEVFRRTLADTLTDLLGATVDEADSGQAALSRLDDS
                     GDVDLVLVDMRMPEMDGLEVSRRILAREQPPRIIVVTAFDERAYSEQAKRIGVDGFFC
                     KTDGVAGLLAEIERVTA"
     gene            5386..5688
                     /gene="clpS"
                     /locus_tag="VNU01_01395"
     CDS             5386..5688
                     /gene="clpS"
                     /locus_tag="VNU01_01395"
                     /inference="COORDINATES: protein motif:HMM:NF000668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWH31305.1"
                     /translation="MSTAVPQVAPDTDERTRTDSREEHDLPWDVIVWNDPVTLMSYVV
                     FVLRKLFGHSEQTATKLMLRIHHEGKAVVSSGPREQAESDVARLHAHGLQATLARG"
     gene            5688..6185
                     /locus_tag="VNU01_01400"
     CDS             5688..6185
                     /locus_tag="VNU01_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HWH31306.1"
                     /translation="MALSRSGVRRISGGVRVRLTPDERDVLATIPAQLRDVFTGEAGS
                     DDLLTRLFPPAYDDALAEAEYHLTRGGELGARRSETLDRYLTSFAKGDDHRAHWVVDL
                     DWDEAHAWLQVVNDSRLVLATAAGIRTESDWEAPIDTPERLLLHWLGWFQEQLVSALM
                     STLDE"
BASE COUNT          879 a         2325 c         2385 g          810 t
ORIGIN      
        1 gcaccgtgga ctacctgctg gccgacgacg cccggggcct gctgtggctg gccaacctcg
       61 gctgcatcga gatgcacccg tggcacagcc gcgtcgacga catcggccat cccgactacg
      121 cgttcttcga cctggacccg atggacgtga gcttcggcac ggtgcgcgag gtcgcgctgc
      181 tggtgcgcac ggtgctggac cagctcggcc tgcgcggcta cccgcgcacg tccggcgcca
      241 ccggcatcca ggtctacgtg cccctggagc gcgtgcactc cgccgcgcgc atccgcgact
      301 gggtcgggcg cgtgtgccgg ctgatcaacc gggcggaccc ggagcgcacc accatggaat
      361 gggcggtcgc cgaccgcacc ggcaaggtgt tcctggacca caacatgaac accgagggcc
      421 gcaacatcgc cgccgcctac tccgtccgcc cggagcgccg cgccacggtg tccgcgcccg
      481 tcacgtggga ggagctggag ggcgacgtgg agcccggcga cttcaccatg gcctcgatct
      541 gggcgcggct ggacgaggtc ggcgacctgt tcgcgccggt gctcgcgggc gggcaggacc
      601 tgcgggcggc catggaggcg gtgggcatgc cggcggagtc ggccgacgac gaggagcggg
      661 gccacgacgt ggggccggtg ctcgacggcg gcgacgagtc gccggcggcc cgcttgcgcc
      721 gctaccagtc ggagcgcgcc ggcgaggaac cagcggccgc gcgacgcctg cgcacctacg
      781 cggccaagcg tgacttctct gtcacgcccg aaccccgggg tgtgtcgaca gagtcatcct
      841 cggatggact gagtcgacac accccgggtg agcagcggtt cgtgatccag caccacctcg
      901 ccacgcgcct gcaccacgac ctgcgcttca cccgcgacgg cgtcgcgccg agctgggccc
      961 tgcccaaggg gctgccggac gtgccgggag tccgccacct cgcggtgcag acggaggacc
     1021 acccgatcga ctacatgacc ttctccggcg agatcccgga gggcgagtac ggggccggtc
     1081 cggtgcgcat ctgggactcg ggcacctacg aggcgctgga gtggagccag gacaaggtca
     1141 gcgtgcggct gcacggcggc cgccaccgcg gcgagtggca cctgttccgc atcggcgggc
     1201 aggacgagcc ggcggtctgg aacgtcatcc gctccgacac gccggacgag gccgagctgc
     1261 cggccgagcc ggccgcctgc gagcccatgc tcgccaccct gaccgagcgc cccttcgacg
     1321 acccgaagtg gctgttcgag gtgaagtggg acggcgtgcg cgcgatcgcc accgtcatgc
     1381 ggcccacgca gggccaggac ggctggaccc ggctggtcag ccgcctgggc aacgacgtca
     1441 gcgccggcta cccggagctg gaggggctgt gggagcgcgt cctggcgcgc ggggccgtgc
     1501 tggacggcga gatcgtcgcc ttcgacgagc gcggcaaccc cagctttcag cggctccagc
     1561 ggcgcatgca cgtgcgcggc gcggcgctgg agcgcctccg ccgccaggtg ccggtgacct
     1621 acgtggcgtt cgacctgctg tggtgcgacg gcgagtcgct gctggaccgc cccctccagg
     1681 agcgcctggc gcgcctggag gacgtcctgg tccccgggac gcacgcgcag cgcagcgagg
     1741 cgatcgccga gcacgggctg gccctgtacg acgcggcgcg cgagcgcggc ctggagggcg
     1801 tcatcgccaa gcgcctggac agccgctacc agcccggcag gcgcagccgc gactggctga
     1861 agctcaaggt gcgtcggcgc gtggactgcg tgatcgccgg ctggctgccc ggcgagggcg
     1921 cccgggcgcg gctgggcagc ctgctgctgg gcatgtggga gggcgaccgc ctgcgctacg
     1981 tgggcaaggt gggcacgggg ttcgaccagg ccgagctgga gcggctgcgc gccctgctcg
     2041 acgagcgcgc caccgatgaa cgcccgttcg ccaaggccgc gcccaacccg ccgaggccgt
     2101 cgaagtgggc gcggccggaa ctggtgtgca ccgtcgagta cgccgaggtc accgagggcg
     2161 gccagctgcg cgcaccgtcg tacaagggca tcgtcgaagg cgccgacccg cgcgcgtgtc
     2221 gctacgagga cctgccgctg tcgacgtgag cgggcgagga ccccagctcc ccggcgcggc
     2281 ggcgccacca gcgcaccagc gacagcagga tcaccagcgc cgcgacgacc gcgcccagca
     2341 ggatgaccac cacgggcgga ctcggctcgc cggtgcccgg cgcggcccag tcctccagtg
     2401 ccgctcgcac ctcgtcgtcg gcgtcgaggg tgccctccca cctcgccgtg ccgccttcgt
     2461 agtccagctc gaccgcgacg tggtgcgcgt cgtcgaggtc ggcgaagggc cactccacgg
     2521 gcaggccgaa cgacgtgccg ggcacgatgg tctcgacctg gagcgcctgg gcggcgggct
     2581 cgccgtcgac cagcacgcgg gcttgcgcct tggtcagcag cgtgccggtg ttggcgacgg
     2641 tgacctccag gaagggtccg ctggggcgcg ccgcgatccg gaccgccttg acctccagct
     2701 cgtgtgcggc ggaccccggc acggtcacct gcacggcgat cacctgacgg gtctcgatga
     2761 ccacgcccgc accgggcccc tcgccggcgg gcggctcgtc acccgcgccg ggcgcccaca
     2821 ccgcgatgcc ggccaggtgg tcccccggcc gcgcgtcgcc gggcaccgcc accgtgaacg
     2881 ggacgacttc gccctggccc ggcgcgagct cgaccgtctc gcgggacagg tcgagccagc
     2941 ggccgacccg ctccagctcc tcgcccggca ggctgtagga gaccccgccc tgctgcgcgg
     3001 tctgggcgtc cgccgcggcg atgcgcagcg tgagcggccc gccggagtcg ttgcggacag
     3061 ccagctcgcc ggacagcgcc gcacccggcg cgacgtcgtc gtacaggaac cagccgccgt
     3121 ccgcggtgga cgagccatcg gcggggcgcg ccacgaacgc cgtcgacggc tcagcggcgt
     3181 gcgcaacggg aaggaggagt tgcaggaccg cggtcgtcgc cacggctgcc agagtcagtg
     3241 cgcgtcgcat gggaagtcca agatgctcgg gggccaggaa ggggaaacgc ccctggacgt
     3301 cggtggcggc caggggcgtc ggcgcccgtc gggagccggc ggggcaccgg ctcccgcggc
     3361 gggtgctacg gcgtggtcgc cagggtgacc gtcacgtcgc tgcggtaggt cttggcgtag
     3421 gtgttcggcg gcacggtcag cgtcatcgtg gtggcgccga agtcgtactt gcccatgccc
     3481 gtgtccaccg cggcggacgc gatcttcacg gcgctgccgg cgtcgatggt gtacggcccg
     3541 gccgtgatcg acggcgccgc cgaggtggtg ccgtccgcgg ccacggtggg ctgggacagc
     3601 gccagcgagc tcgtggcgat ggtcttgccg ccggtcacgt agccgccggc gccggcgttc
     3661 agggccccgt cccactcctt gagctgggtt ccctggacgg tgatgttcca gcccgcgccg
     3721 gtgccgcggg agtcgttcac ctcgaacgcg gggaccgccg cggtcgtggt cttggtggtg
     3781 ccgtcgaggg tgagggcgga gaagtcgccc gccaccggcg cggacgtgaa cgcgaggctg
     3841 ccgccggtga cggcgacctc ggaggcggcg gcgggggcgg cgatggcgcc ggccagggcg
     3901 acggtggcgg cgacagccgc gatcttgcgc ttcaacacgg tggaactccc ttggaaggtt
     3961 gcggacgttc ggactgcgcc gtctcgcaag gcggcgcatg ccccctgtgc ctcgggggcc
     4021 gcagtgagcg gctggagtgc tccgggcgta aaagcgcccg gtccgaacga ggtgcgtatg
     4081 cccggagtgc cccaatgcgc acccgagcgg gggaaggtgc gcactcgagg gactccaagg
     4141 cgtcagatcg cccccgggac catcacgggc gcgcgaccgc gtgccgacag agccagccat
     4201 ggactcaccc ggatcgctcg tgctgcgtgc cctgcggcgt accctcccgt tcacggcgcc
     4261 ggtggcgccg gccatcctcc aggcatggga tggcggcgcg ccccagtggg ccgcggaagc
     4321 cgcgctgcgc cagctcggcg tcggcccgct gggcggtcag ccctccgcga gcaggcgggc
     4381 gaacgggcgg ttcatggcgt ccagtgcgga gcgcacggcc gccacggcga ggtcgccgcg
     4441 cgagagcgcc gagccgacgt agaggtcgcg accgcgctcg ctgcggcagg cgaccgccgt
     4501 gtgcagcacc acatggccga agtggccgtc cggctccagc accttgaacc aggccagctc
     4561 cagcgtcgcc cgcccgtcca ggagctcgcc cagcgcgctg agcgccgcct ccgcggccgt
     4621 gtgcgcgacc ccgcgctcgt cgcccgggcc ctgagctcgc gacgtgacca cgcgcccgcc
     4681 cagcgacagc gtcacgtcga cctccgtgga cgtgggcccc gggaacacct ggagctcggc
     4741 gaacaccggc cggtagcggt ccctgcccac ggcggcaccg ggcgcctcgc cgagcaccgg
     4801 atcgaacccg tctccctctt gacgctcccg cacgccgtcg gtcatccctg caccatagcc
     4861 ggacagccgc aagaacaggg cttagggtag ggaccgacgc tgccgacact cacgaggcgg
     4921 cggcccttcc cgggggtgcc gacgtcgcac tgacccacca ccgtgagcag ggggatgcca
     4981 ccaccgtgcg cgtgttgctc gtcgacgact ccgaagtgtt ccgccggacc ctggcggaca
     5041 ccttgacgga cctgctcggc gccaccgtgg acgaggccga cagcgggcag gcggcgctca
     5101 gccggctgga cgactccggc gacgtggacc tggtcctggt ggacatgcgc atgccggaga
     5161 tggacggcct cgaggtgtcg cggcgcatcc tcgcccgcga gcagccgccg cgcatcatcg
     5221 tggtcaccgc gttcgacgag cgcgcctaca gcgagcaggc caagcgcatc ggcgtggacg
     5281 gcttcttctg caagaccgac ggcgtggccg gcctgctcgc cgagatcgag cgcgtcaccg
     5341 cctgagcgga cggaccgccc gcgcgcgtga gctagcctgc aggccatgtc caccgcagtg
     5401 ccgcaggtgg cgcccgacac cgacgagcgg acgcgcaccg actcccgcga agagcacgac
     5461 ctgccgtggg acgtcatcgt ctggaacgac cccgtgacgc tcatgagcta cgtggtgttc
     5521 gtgctgcgca agctgttcgg ccacagcgag cagaccgcca cgaagctcat gctgcggatc
     5581 caccacgagg gcaaggccgt ggtgtccagc gggccgcgcg agcaggccga gtccgacgtc
     5641 gcgcgcctgc acgcccacgg gctgcaggcc acgctggcgc gcggctgatg gcgctgtcgc
     5701 gcagcggcgt gcgccgcatc tccggcggcg tgcgcgtacg gctcacgccg gacgagcgcg
     5761 acgtgctggc caccatcccg gcgcagctgc gcgacgtgtt caccggcgag gcaggcagcg
     5821 acgacctgct cacgcgcctg ttccccccgg cctacgacga cgccctcgcc gaggcggagt
     5881 accacctgac ccgcggcggc gagctcggcg cgcgccgcag cgagacgctg gaccgctacc
     5941 tcaccagctt cgccaagggc gacgaccacc gcgcccactg ggtggtggac ctggactggg
     6001 acgaggcgca cgcgtggctg caggtggtca acgacagccg cctggtgctg gccacggcgg
     6061 cgggcatccg caccgagtcg gactgggagg ctccgatcga cacgccggag cgcctgctgc
     6121 tgcactggct cggctggttc caggagcagc tcgtgtccgc cctgatgtcg accctggacg
     6181 agtagcgacg cgaaggcccc cggcgggggg atgccggggg ccttcgcttg gggtggactg
     6241 tcgaagcctg ccggcagcct actggccgcc ggcgaagtgg cgctcgacgt ggcgcgagat
     6301 gacgagctgc tggatctcct tggtgccctc gaagatctcg tagatcttgg agtcgcggtg
     6361 ccacttctcc accgggaagt ccttgacgta gccgtagcc
//
LOCUS       DATMKC010000051        15149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_231959, whole genome shotgun sequence.
ACCESSION   DATMKC010000051 DATMKC010000000
VERSION     DATMKC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231959"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VNU01_01405"
     CDS             complement(<1..775)
                     /locus_tag="VNU01_01405"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HWH31307.1"
                     /translation="MSELTKILLDESEMPTQWYNVVPDLPEPPPPVLHPGRMDPVGPD
                     DLAPLFPMALILQEVSQDRYIDIPEAVQEVYKLWRPSPLYRARRLEAALGTPARIYYK
                     YEGVSPAGSHKPNTAVPQAYYNAQEGIRRLTTETGAGQWGTALALACSMFGLECEVWQ
                     VRASYDQKPYRRMMIETFGATIHPSPSDVTEYGRKVLADDPDSPGSLGIAISEAVEVA
                     AGNDDTRYALGSVLNHVLLHQTVIGEEALKQLAKVGESPD"
     gene            888..2120
                     /gene="glgC"
                     /locus_tag="VNU01_01410"
     CDS             888..2120
                     /gene="glgC"
                     /locus_tag="VNU01_01410"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWH31308.1"
                     /translation="MAGGHVLAIVLAGGEGKRLAPLTADRAKPAVPFGGNYRLVDFVL
                     SNLVNAGYRRVIVLTQYKSHSLDRHIARTWAMTPLLGQYVATVPAQMRKGPRWFAGSA
                     DAIFQNLNLVEDERPDHVLVFGADHIYRMDPRPMVAEHIASGAAVTVAGIRVPIGQAD
                     QFGVIEPGADGRIATFLEKPSDPVGLPDAPEQVFASMGNYVFTTEALCQAVTEDAANP
                     ASKHDLGGDIIPMLVERGEAAVYDFSANVVPGETERDHGYWRDVGTIDAYYDAHMDLV
                     SVHPIFNLYNRDWPIHASRESLPPAKFVFDEADRRGMAVNSIVSAGAVVSGGTVRRSV
                     LSPRAFVHSYAVVEDAVVLTGAEVGRGAVVRRAILDKNVVVESGAEIGVDLARDRSRF
                     TVSANGIVVVGKGVRVEP"
     gene            2131..3282
                     /gene="glgA"
                     /locus_tag="VNU01_01415"
     CDS             2131..3282
                     /gene="glgA"
                     /locus_tag="VNU01_01415"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HWH31309.1"
                     /translation="MRVALLSKEYPPEVYGGAGVHVAGLSAELAKLVDVAVHAFGAPR
                     DDALVAATYQPWDALAGDAPGLGALRPISVDLAMAAAVGDADVVHSHTWYANLGGHLA
                     KLLHGVPHVMTSHSLEPQRPWKAEQLAGGYAVSSWCERVSIEAADAVVAVSAGMRDDI
                     LACYPAVDADRVHVIHNGIDPSLWRPEAAPNVLALRGIDPERPTVAFVGRITRQKGVP
                     DLLRAAASFDPAAQLVVLAGRPDTPEIGREVRELVAGLQAERTGVVWIEDMLPLEQVR
                     PILSHATVFVCPSVYEPFGLVNLEAMACGTAVVATRVGGIPEIVVEEETGLLVAPADA
                     SGMAAAVNRLVGDPDLAARMGAAGRARVEQHFTWASVAQRTADLYRSLT"
     gene            complement(3353..5050)
                     /locus_tag="VNU01_01420"
     CDS             complement(3353..5050)
                     /locus_tag="VNU01_01420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWH31310.1"
                     /translation="MNPQPMNSARVGPTERAAAIARMRAEVFDVLVVGGGVTGCGTAL
                     DAVTRGLSTALVEARDFAAGTSSRSSKLIHGGLRYLEQYNFALVHEALKERALILDRL
                     APHLVQPVPFLYPLTRPVWERAYVGAGVLLYDLMAGLSQGDRALPRHRHLSRRQALKL
                     VPSLREDALVGAVQYWDAQVDDARHTMTLARTAALYGAQLATSVRVYGFLREGERVVG
                     VRARDLETGEELDIRARQVINATGVWTDDLQELVGRGRIRVRASKGIHLVVPRDRIHS
                     DTGLILRTESSVLFVIPWGRHWIVGTTDTDWDLDKAHPAASRSDIDYLLARVNRELRS
                     PLTHEDVEGVYAGLRPLLYGESEATSRLSREHAVSHSVPGLVTVAGGKYTTYRIMAMD
                     AVDAAARSLDRRVPASVTDRTPLLGAVGYQAAWNQRDRLAQESGLHIARIEHLLRRYG
                     SRIGELLDLVAADPALGRPIRGADDYLAVEAVYAASHEGALHLDDILTRRTRISIETF
                     DRGVAAAEHVAELVAPVLGWDDDARVAAERDSQQQPDDHTADAARLGAPDVRTGRTA"
     gene            complement(5047..6477)
                     /locus_tag="VNU01_01425"
     CDS             complement(5047..6477)
                     /locus_tag="VNU01_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWH31311.1"
                     /translation="MPPGQHMTPEEFRRHGHAVVDWVADYLERVEQLPVQSRVAPGEV
                     LARLPGEAPVEGEPFDDLLADVDLLILPGITHWQSPNFFAYFPANNSGPSILGELLSA
                     GLGVQGMLWSTSPAATELETAVMDWLARLLDLPEHFLSTGSGGGVIQDSASSATLCAL
                     LAARERAVRAGGDLAALTLYASSQAHSSLEKAVKIAGLPADALRLVDVDTETFAARPE
                     VLLGLIHADREAGKVPFFVQANIGTTSSTAIDPVRELGQIANAEGLWLHVDAAMSGSA
                     AVVPELRWTHDGVELADSYCFNPHKHLLVNFDCDAFWVRDRSALLDALSILPEYLRNA
                     ATESGQVIDYRDWQIPLGRRFRALKLWFTLRWYGQEGLRDFVRGHVALAAELAQWVAA
                     HPDFALLAPAPLTLVCFAHVGGDDATQRIMDTVNASGRAFLTHTRLNGRLALRVSVGQ
                     ARTERRHVEALWQQICRAADGEGSAG"
     gene            complement(6525..6866)
                     /locus_tag="VNU01_01430"
     CDS             complement(6525..6866)
                     /locus_tag="VNU01_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31312.1"
                     /translation="MMQESLRMRRRVAFGRVVGVLFLVAGLAVGAYFAVVQADEPMAL
                     RAVGTGMGLIGAVSGVLVAREFHRLAELPWHDPGVARAKLAQRVEPLASAGTIAAIAC
                     SLAAALASRLS"
     gene            complement(6935..8935)
                     /locus_tag="VNU01_01435"
     CDS             complement(6935..8935)
                     /locus_tag="VNU01_01435"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWH31313.1"
                     /translation="MGGNAPTPTDRIRNVLLVGHGGTGKTTLAETMMATAGTDVRGGL
                     FDFEPEERDKGHSMSLAVASFEWRGHKVNLLDCPGSADFLGDAFPALRAADVALFVVD
                     ATAGVQAQHDQLWEACEAIGLPRIVFVNKLDKDNAHYQPILDALRERAGKAVAPVLMP
                     VAGGELSGVIDLLHFTAVQKVDGQRVESEVPPGMEEVARRNRDALVEAIVEVDDALLE
                     RYLEGEIPEPKELAECFAHGVATSSFFPVLCGSATQAIGHRLLMDFIVEECPPPAAEP
                     GGPLALYVAKTLSDPFVGRINVLKVLSGTLRQDAHAIDARTGADLRLHQLFALQGKEQ
                     APVTEVSAGDLVAVAKLDDVCTGDVLAEKGASAEVERVAPPEPFHRVALEPTSAGDED
                     KLSSALARIAEEDPSLRVERDAETHQLVLRTYGPGHVELTLARMQRKFGVSVRQVPLR
                     LRYRETLRGAATGVGRHVKQSGGHGQYGVCTIEVEPLPRGEGFVFADRIVGGVIPGTY
                     IPSVEKGVRDTMAHGVLAGYPVVDVKVTLVDGKTHSVDSSDMAFQMAGSLAFRDAADK
                     AGMCLLEPVMEVGVVIPDALTGDVMGDLSSRRGRILGTEPAGPGRTTVRAQVPEAELT
                     SYVAELRSLSSGSGSVTLRYDHHAEVPDNIARKIVEAASAAT"
     gene            complement(9007..10122)
                     /gene="serC"
                     /locus_tag="VNU01_01440"
     CDS             complement(9007..10122)
                     /gene="serC"
                     /locus_tag="VNU01_01440"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR01366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HWH31314.1"
                     /translation="MSETTIDIPAELRPRDGRFGSGPSKVRREQIEALAAAGPGLLGT
                     SHRKPAVRNQVKRLREGLAALFELPDGYEVALGNGGSTLFWDAATFHLIRERSAHAVF
                     GEFSSKFAKAAKAAPHLGDPVIFESEPGTHPAVEAADGVDAYALTHNETSTGVAMPLR
                     RPAGDALVLVDATSGAGGIAFDPREVDAYYFAPQKAFASEGGLWLAVLSPAAIARIEE
                     LSGRWAPEILNLSTALDNSRQEQTYNTPSLSTIFLMAEQVDWMNAQGGLAWAAKSTAA
                     KSGHVYDWAESREWATPFVQVPEQRSPVTCTIDLDASVPADDVTRILRANGILDIDAY
                     RKLGRNQVRIATFPAIEQADVEALTACIDYVVDRLRS"
     gene            10179..11345
                     /locus_tag="VNU01_01445"
     CDS             10179..11345
                     /locus_tag="VNU01_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31315.1"
                     /translation="MHPFASPQGGADMPPPGHLRVLGGAQDVAVAAATPVELAGARLY
                     RLDDGRVLAPAFRRFAVDLGAGALPDEADEDALGALVLRWVEAVRDHDWTPYRGAELE
                     GGEDWADLRAAMVRLGWGPDAAVAAWEACRQRPYSRDLPTAAGWAAAGWVVPASADEP
                     EPAFARPVLEARGLYRWGYIQRWAAPAAIPELPLPVEPEPADLGWGDVLSRLRQDHHT
                     QDSLAEAEQLAIEILELAGLGPRNPVEAEDEPLPDRDVAAACAHASPLVRAAAWFLLR
                     DEAEAWLPCTYNECAHHWAVEAEEDAFEMEEVLELASRGVANPVDAAFQRRRLLTLAF
                     GDISDVPLAGAGDLDDFIAFYTEWTAHGQPEEAFEEMVEELRGDSPLDGPDASG"
     gene            11388..12311
                     /locus_tag="VNU01_01450"
     CDS             11388..12311
                     /locus_tag="VNU01_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31316.1"
                     /translation="MASRTARALLGGTIALLLFTGVTLAYVRQPVAPVSADTALERFR
                     SAAPEPQASPVATPEPGSSAQPAEAAPSEPVATTPDPAGPTAAAPAPSPAPPPAAGGP
                     APMPKPRAGVYSYRTTGYEEVDALGGSRHDYPETTYMTIRDKGCGVVMRWQPLEERWD
                     ESDVCPEGADIALHRFTTYHEFFQQGQAQAYDCAPGSHVHRGGAPPGSEWTWRCQGEH
                     GAIQTSVRVVGPEPVTVGGTTVEAMRVRYSSVATGSSRGVQEQERWFDLRSGLLVRIQ
                     TDAMLQVDTPFGPTGYREQYRIDLLSLEPQT"
     gene            complement(12376..13344)
                     /locus_tag="VNU01_01455"
     CDS             complement(12376..13344)
                     /locus_tag="VNU01_01455"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWH31317.1"
                     /translation="MTIQATELQVPWGLAISANGRVFFTERDTGVVSELSQGRPRPVR
                     GIDGVVPGGEGGLLGIALSPEFADDRLVYAYFTGAEDNRVVRFALDRPDPVQVLVRGI
                     PKAGVHNGGRIAFGPDGMLYVGTGDAGRSELAQSRGSLAGKILRVRPDGSVPPDNPFP
                     GSPVWSLGHRNVQGLAWDGRGRMWAAELGPDRDDEVNQIVRGGNYGWPQVTGATRADG
                     FRPALAVKQPSEASWSGLAFVAGEAFPEWGDSLVASGLRGGRLWRFPITPEGGLGPGE
                     ELFGGEFGRLRAVVSAPDGALWFTTSNRDGRGEPAAEDDRILRYGR"
     gene            complement(13514..13717)
                     /locus_tag="VNU01_01460"
     CDS             complement(13514..13717)
                     /locus_tag="VNU01_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31318.1"
                     /translation="MRRRLPATVSALAMAAAVAGCSGGDSSPEDAAGGGIPPQSSVEP
                     RTNIGQSEGFPDAGAQQEATTDE"
     gene            complement(13767..15116)
                     /locus_tag="VNU01_01465"
     CDS             complement(13767..15116)
                     /locus_tag="VNU01_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH31319.1"
                     /translation="MTEDTLDPPEASVRRVALLLGTLVALTVVSSSAIAVALPTVRED
                     LGLDGPGTAWVIAAYSLSFAITTAIFGRLADLHGLRRPMRIGIVLFTAGSLVGATAPS
                     FPVLMAGRVLQGMGAGAVPVIGLGIIAARFTDAARGKALGAITAVVSIVSGAGPLIGG
                     GVAQLAGWRWVLALPAIALLFAEPVARLAPREPMGSGRLDVRGAALVALAVTAFTMVL
                     QSPSTGIGLRPALGLAAVAVAGFAFLAAHVRARPAGLLPVRVLRNPLVVLNGLAGLTA
                     LAAYLGAMLAVPQILARDQGWEPLHIGLVLLVPAAVGAVTSRTVGGVAARLGRSRVAA
                     ILIGGSAAGLVVVGLFHAVPVALLAGMALVFMGFGGGQVALLDSLTNGVEHDVRGIAL
                     GVFNLVFFVGGAVGSATVGGLSEVVSLPRALMVLALLPAVGALLAARLVRVEQGYRP"
BASE COUNT         2018 a         5613 c         5571 g         1947 t
ORIGIN      
        1 ggtcggggct ctcccccacc ttggcgagct gcttgagcgc ctcctcgccg atgaccgtct
       61 ggtgcagcag cacgtggttg agcacgctgc cgagcgcgta gcgcgtgtcg tcgttgcccg
      121 cggcgacctc caccgcctcg ctgatggcga tgccgagcga acccggcgag tccgggtcgt
      181 cggcgagcac cttgcgcccg tactcggtga cgtcggaggg gctcgggtgg atggtcgcgc
      241 cgaacgtctc gatcatcatg cgccggtacg gcttctggtc gtagctggcg cgcacctgcc
      301 acacctcgca ctccagcccg aacatggagc acgcgagcgc gagcgcggtc ccccactgcc
      361 cggcgccggt ctccgtggtg agccggcgga tgccctcctg ggcgttgtag taggcctgcg
      421 gcacggcggt gttgggcttg tgcgagccgg caggcgagac gccctcgtac ttgtagtaga
      481 tgcgcgccgg cgtgccgagc gccgcctcga gccgccgcgc gcggtacagc ggcgagggcc
      541 gccacagctt gtagacctcc tggaccgcct cggggatgtc gatgtagcgg tcctggctga
      601 cctcctggag gatcagcgcc atcgggaaca gcggcgcgag gtcgtcgggg ccgacggggt
      661 ccatgcggcc ggggtgcagc accggcggcg gcggctccgg caggtccggc acgacgttgt
      721 accactgcgt cggcatctcg gactcgtcga gcaggatctt ggtcagttcg ctcatcgcag
      781 gtccgtcccc atggtgtcca gggccgctcc cccaggcggc cggccgctgt gaagggcgct
      841 caccatagcc gcgtctggac ccggcgggaa cgggtaatgt gcgcgccatg gcaggcggac
      901 atgtgctcgc gatcgtgctc gcgggcggcg agggcaagcg gctggccccg ctgacggccg
      961 accgcgccaa gcccgccgtg ccgttcgggg gcaactaccg cctggtcgac ttcgtgctgt
     1021 ccaacctggt caacgccggc taccggcgcg tcatcgtgct gacgcagtac aagagccaca
     1081 gcctggaccg ccacatcgcg cgcacgtggg cgatgacgcc gctgctcgga cagtacgtcg
     1141 ccaccgtgcc cgcgcagatg cgcaaggggc cgcgctggtt cgccggctcg gccgacgcga
     1201 tcttccagaa cctgaacctg gtggaggacg agcgtcccga ccacgtgctg gtgttcggcg
     1261 ccgaccacat ctaccgcatg gacccgcgac cgatggtggc cgagcacatc gcctccggcg
     1321 ccgccgtcac cgtggcgggc atccgcgtcc cgatcgggca ggccgaccag ttcggcgtca
     1381 tcgagccggg tgcggacggg cgcatcgcca ccttcctgga gaagccgtcc gaccccgtgg
     1441 ggctgcccga cgcgccggag caggtgttcg cgtccatggg caactacgtg ttcaccacgg
     1501 aggcgctgtg ccaggcggtg acggaggacg ccgccaaccc cgcgtccaag cacgacctgg
     1561 gcggcgacat catccccatg ctggtggagc gcggcgaggc ggccgtgtac gacttctcgg
     1621 cgaacgtggt gcccggggag accgagcgcg accacggtta ctggcgcgac gtcgggacca
     1681 tcgacgccta ctacgacgcg cacatggacc tggtgtccgt ccacccgatc ttcaacctgt
     1741 acaaccgcga ctggccgatc cacgcgtccc gggagtcgct gcccccggcg aagttcgtct
     1801 tcgacgaggc cgaccgccgc ggcatggcgg tcaactccat cgtgagcgcg ggcgccgtcg
     1861 tgtccggcgg cacggtgcgc cgctccgtgc tgtcgccgcg ggcgttcgtg cacagctacg
     1921 ccgtcgtgga ggacgccgtg gtgctgacgg gcgccgaggt cggccgcggg gccgtggtgc
     1981 gccgcgcgat cctggacaag aacgtggtgg tggagtcggg cgcggagatc ggcgtggacc
     2041 tggcgcgcga ccgctcccgc ttcacggtgt cggccaacgg catcgtggtc gtgggcaagg
     2101 gcgtgcgggt ggagccatga ggacgccgcg gtgagggtcg cgctgctgag caaggagtac
     2161 ccgccagagg tgtacggcgg tgccggggtg cacgtcgccg ggctgtccgc ggagctcgcg
     2221 aagctggtcg acgtggcggt gcacgcgttc ggcgcgccgc gcgacgacgc gctggtggcc
     2281 gcgacctacc agccgtggga cgccctggcc ggtgacgcgc ccgggctcgg cgcgctgcgg
     2341 ccgatctccg tggacctggc catggccgcc gccgtcggcg acgccgacgt ggtgcacagc
     2401 cacacctggt acgccaacct cggcgggcac ctggccaagc tgctgcacgg cgtgccgcac
     2461 gtgatgacgt cgcacagcct ggagccgcag cggccgtgga aggccgagca gctcgccggc
     2521 ggctacgccg tgtcgagctg gtgcgagcgc gtgtccatcg aggccgccga cgccgtcgtc
     2581 gccgtgtcgg ccggcatgcg cgacgacatc ctcgcgtgct acccggcggt cgacgccgac
     2641 cgcgtgcacg tgatccacaa cggcatcgac ccttcgctgt ggcggcccga ggccgcaccg
     2701 aacgtcctgg cgctgcgcgg catcgacccg gagcggccga ccgtggcgtt cgtcgggcgc
     2761 atcacgcggc agaagggcgt gcccgacctg ctgcgggccg cggcgagctt cgacccggcg
     2821 gcgcagctcg tcgtgctggc gggtcgcccg gacacgccgg agatcggccg cgaggtgcgc
     2881 gagctggtcg cggggctgca ggccgagcgc accggggtcg tgtggatcga ggacatgctg
     2941 ccgctggagc aggttcggcc catcctcagc cacgcgaccg tgttcgtgtg cccgtccgtg
     3001 tacgagccgt tcggcctggt gaacctggag gccatggcgt gcggcacggc ggtcgtggcc
     3061 acccgcgtcg gcggcatccc ggagatcgtg gtcgaggagg agaccgggct gctggtcgcg
     3121 ccggccgacg cgagcggcat ggccgccgcg gtcaaccgcc tggtgggcga cccggacctg
     3181 gccgcgcgca tgggcgccgc cggccgcgcc cgcgtcgagc agcacttcac gtgggcgtcg
     3241 gtggcccagc gcaccgccga cctctaccgc agtctcacct gaccgtcctg cccgctccgg
     3301 gaccacctgt ggccgatagc ggacacaact cgtcccgcaa cggcgagggg ggtcaggcgg
     3361 tgcggccggt gcggacgtcg ggggcgccca ggcgcgcggc gtcggcggtg tggtcgtcgg
     3421 gctgctgctg gctgtcgcgc tccgccgcca cgcgcgcgtc gtcgtcccag ccgagcaccg
     3481 gcgccaccag ctccgcgacg tgctcggccg ccgcgacccc ccggtcgaag gtctcgatcg
     3541 agatgcgcgt gcgccgggtc aggatgtcgt cgaggtgcag cgcgccctcg tgcgacgccg
     3601 cgtacacggc ttccaccgcc aggtagtcgt cggcgccgcg gatcggccgt cccagcgccg
     3661 ggtccgcggc caccaggtcg agcagctcgc cgatgcggga gccgtagcgg cgcagcaggt
     3721 gctcgatgcg cgcgatgtgc aggccggact cctgggccag ccggtcgcgc tggttccacg
     3781 ccgcctggta gccgaccgcg ccgagcagcg gcgtgcggtc cgtcaccgac gccggcacgc
     3841 gccggtcgag gctgcgcgcc gcggcgtcca cggcgtccat cgccatgatg cggtaggtcg
     3901 tgtacttgcc gcccgcgacg gtcaccaggc ccggcaccga gtgcgacacc gcgtgctcgc
     3961 gcgacagccg cgaggtcgcc tcgctctcgc cgtacagcag cgggcgcagg cccgcgtaca
     4021 cgccctccac gtcctcgtgc gtgagcgggc tgcgcagctc gcggttcacg cgtgcgagca
     4081 ggtagtcgat gtcgctgcgg cttgcggccg gatgcgcctt gtcgaggtcc cagtcggtgt
     4141 cggtggtgcc gacgatccag tggcggcccc acgggatcac gaacagcacg ctggactcgg
     4201 tgcgcaggat caggccggtg tcggagtgga tgcggtcccg cggcaccacg aggtggatgc
     4261 ccttggaggc gcgcacgcgg atgcggccgc gcccgaccag ctcctgcagg tcgtcggtcc
     4321 acacgcccgt ggcgttgatg acctggcgcg cccggatgtc gagctcctcc cccgtctcca
     4381 ggtcgcgcgc ccgcacgccg acgacgcgct cgccctcgcg caggaagccg tacacgcgca
     4441 cgctggtcgc gagctgcgcg ccgtacagcg ccgccgtgcg cgccagcgtc atggtgtggc
     4501 gcgcgtcgtc gacctgcgcg tcccagtact gcacggcccc caccagcgcg tcctcgcgca
     4561 gcgacggcac cagcttcagg gcctggcgcc gcgacaggtg gcggtggcgc ggcagcgccc
     4621 ggtcgccctg cgacaggccg gccatgaggt cgtagagcag cacgcctgcg cccacgtagg
     4681 cgcgctccca caccggcctg gtgagcgggt acaggaacgg caccggctgc accaggtgcg
     4741 gggcgagccg gtccaggatc agggcgcgct ccttgagcgc ctcgtgcacc agcgcgaagt
     4801 tgtactgctc caggtagcgc agcccgccgt ggatgagctt gctggagcgg ctggacgtgc
     4861 cggcggcgaa gtcgcgggcc tcgaccagcg ccgtggacag cccgcgggtc accgcgtcca
     4921 gcgccgtgcc gcagccggtc accccgccgc cgacgaccag gacgtcgaac acctcggcgc
     4981 gcatgcgggc gatggcggcg gcccgctcgg tgggaccgac gcgcgcggag ttcatcggct
     5041 gcgggttcac ccggccgagc cttccccgtc cgccgcccgg cagatctgct gccacaacgc
     5101 ctccacgtgc cgccgctcgg tgcgcgcctg gcccacggag acgcgcagcg ccagtctgcc
     5161 gttcaggcgc gtgtgcgtga ggaaggcgcg cccggacgcg ttgacggtgt ccatgatgcg
     5221 ctgcgtcgcg tcgtcgccgc cgacgtgggc gaagcagacc agcgtgagcg gcgcgggcgc
     5281 gagcagcgcg aagtccgggt gcgccgccac ccactgcgcc agctccgccg cgagcgcgac
     5341 gtggccgcgc acgaagtccc gcagcccctc ctggccgtac cagcgcagcg tgaaccacag
     5401 cttcagcgcg cggaagcgcc ggcccagcgg gatctgccag tcgcgatagt cgatcacctg
     5461 cccggactcg gtcgcggcgt tgcgcaggta ctcgggcagg atcgacaggg cgtcgagcag
     5521 cgccgagcgg tcgcgcaccc agaacgcgtc gcagtcgaag ttcaccagca ggtgcttgtg
     5581 ggggttgaag cagtagctgt cggcgagctc cacgccgtcg tgggtccagc gcagctccgg
     5641 caccacggcg gcgctgccgc tcatggcggc gtcgacgtgc agccacaggc cttcggcgtt
     5701 ggcgatctgg ccgagctcgc gcaccgggtc gatggcggtg gacgaggtgg tgccgatgtt
     5761 ggcctggacg aagaacggga ccttgccggc ttcgcggtcg gcatggatca ggccgagcag
     5821 cacctcggga cgggcggcga acgtctccgt gtcgacgtcg accaggcgca acgcgtccgc
     5881 gggcaggccc gcgatcttca cggccttctc cagcgacgag tgcgcctggc tggacgcgta
     5941 cagcgtcagc gccgccaggt ccccgcccgc gcggacggcg cgctcgcggg cggccagcag
     6001 cgcgcacagc gtggcgctgg aggcggagtc ctggatcacc cctcccccgc tgcccgtgga
     6061 caggaagtgc tcgggcaagt ccagcagccg cgcgagccag tccatgacgg cggtctcgag
     6121 ctccgtggcg gccgggctgg tcgaccacaa catgccctgc acgccgaggc cggcggacag
     6181 cagctcgccc aggatggacg ggccggagtt gttggcaggg aagtaggcga agaagttcgg
     6241 cgactgccag tgggtgatgc ccggcaggat cagcaagtcg acgtcggcga gcaggtcgtc
     6301 gaagggctcg ccttccacgg gggcctcgcc gggcaggcgc gcgagcactt cgccgggggc
     6361 gacgcgggat tgcacgggga gctgctcgac ccgctcgagg tagtccgcga cccagtcgac
     6421 gaccgcgtgg ccgtggcggc ggaactcctc cggggtcatg tgctgacctg ggggcatggc
     6481 cgccagccta ccggcccacg ttgcacgctt gggggcggcg gccgctagct caggcgggag
     6541 gcgagcgccg cggccaggga gcaggcgatg gcggcgatgg tgccggccga cgccagcggc
     6601 tcgacgcgct gggcgagctt ggcgcgcgcc acgcccgggt cgtgccacgg cagctcggcc
     6661 aggcggtgga actcgcgggc gaccagcacg cccgacacgg cgccgatgag gcccatgccg
     6721 gtgccgacgg cgcgcagggc catcggctcg tcggcctgga ccacggcgaa gtaggcgccg
     6781 acggcgagac cggcgaccag gaagagcaca ccgaccactc gcccgaacgc cacccgccgc
     6841 cgcatgcgca gcgactcttg catcatgcgg ccacggtaat ccggccgggc gccttgactc
     6901 cagccgcggt gggaactttg acgccgcggc gggtttacgt cgctgcggat gcggcctcga
     6961 cgatcttgcg cgcgatgttg tccggcacct cggcgtggtg gtcgtagcgc agggtgacgc
     7021 tgccgctgcc cgacgacagc gaccgcagct cggcgacgta gctggtcagc tccgcctccg
     7081 gcacctgggc gcgcaccgtc gtgcggcccg gtccggcggg ctcggtgccc aggatcctcc
     7141 cccgccgcga cgacaggtcg cccatcacgt cgccggtcag cgcgtccggg atcacgacgc
     7201 cgacctccat gaccggctcc agcaggcaca tgccggcctt gtccgcggcg tcgcggaacg
     7261 ccagcgagcc cgccatctgg aaggccatgt cggagctgtc cacggagtgc gtcttgccgt
     7321 cgaccagcgt caccttgacg tccaccaccg ggtagccggc cagcacgccg tgggccatgg
     7381 tgtcgcgcac gcccttctcc acgctgggga tgtaggtccc ggggatcacg cccccgacga
     7441 tgcggtcggc gaacacgaag ccctcgccgc gcggcagcgg ctcgacctcg atggtgcaca
     7501 cgccgtactg gccgtggccg ccggactgct tgacgtggcg gcccacgccc gtcgcggcgc
     7561 cgcgcagcgt ctcgcggtag cgcaagcgca gcggcacctg ccggaccgac acgccgaact
     7621 tgcgctgcat ccgcgccagg gtcagctcca cgtgaccagg cccgtaggtg cgcagcacga
     7681 gctggtgcgt ctccgcgtcg cgctccaccc gcagggacgg gtcctcctcg gcgatgcgcg
     7741 ccagcgcgct ggacagcttg tcctcgtccc cggcgctggt cggctccagg gccacgcggt
     7801 ggaacggctc cggcggcgcg acccgttcca cctcggcgct cgcgcccttc tccgccagga
     7861 cgtcgcccgt gcacacgtcg tcgagcttgg cgaccgcgac caggtccccg gcggacacct
     7921 ccgtcaccgg cgcctgctcc ttgccctgca gcgcgaacaa ctggtgcagg cgcaggtcgg
     7981 cgccggtgcg ggcgtcgatg gcgtgcgcgt cctgacgcag cgtgccggac agcaccttga
     8041 gcacgttgat gcgtcccacg aacgggtcgg acagcgtctt ggcgacgtac agcgccaggg
     8101 gcccgccggg ctcggcggcg ggcggcgggc actcctccac gatgaagtcc atcagcaggc
     8161 ggtggccgat cgcctgcgtg gcggagccgc acagcaccgg gaagaacgag ctggtggcga
     8221 cgccgtgcgc gaagcactcc gccagctcct tgggctcggg gatctccccc tccaggtagc
     8281 gttccagcag ggcgtcgtcg acctcgacga tcgcctccac cagggcgtcg cggttgcgcc
     8341 gggccacctc ctccatgcct ggcggcacct ccgactccac ccgctggccg tccaccttct
     8401 gcacggcggt gaagtgcagc aggtcgatca ccccgctgag ctcgccgccc gcgaccggca
     8461 tgaggaccgg cgcgaccgcc ttgccggcgc gctcgcgcag cgcgtccagg atgggctggt
     8521 agtgggcgtt gtccttgtcc agcttgttca cgaacacgat gcgcggcagc cctatggcct
     8581 cgcaggcctc ccagagctgg tcgtgctggg cctgcacccc ggcggtcgcg tccaccacga
     8641 acagggcgac gtcggcggcg cgcagggccg ggaacgcgtc gccaaggaag tccgcggagc
     8701 cgggacagtc cagcaggttc accttgtggc cgcgccactc gaagctcgcg accgccagcg
     8761 acatggagtg gcccttgtcg cgctcctccg gctcgaaatc gaagaggcca ccgcgcacgt
     8821 ccgtgcctgc ggtggccatc atggtctctg cgagtgtggt cttgccggtg cccccgtgtc
     8881 cgaccagcaa gacgttgcgg atccggtcgg tgggggtcgg tgcattgccg cccatggtct
     8941 gacacctcct ggctcggtgg tgtcgacatt acgcctgcgc ggcagagctg tgaacccggt
     9001 tttcgctcag gagcgcaggc ggtccaccac gtagtcgatg caggcggtca gcgcctccac
     9061 gtcggcctgc tcgatcgccg ggaacgtcgc gatgcgcacc tggttgcggc cgagcttgcg
     9121 gtaggcgtcg atgtccagga tcccgttggc gcgcaggatc ctggtgacgt cgtcggcggg
     9181 cacggacgcg tcgaggtcga tggtgcacgt caccgggctg cgctgctcgg ggacctggac
     9241 gaagggcgtg gcccattccc gcgactccgc ccagtcgtag acgtggccgg acttggccgc
     9301 ggtcgacttc gccgcccagg ccaggccgcc ctgcgcgttc atccagtcga cctgctcggc
     9361 catcaggaag atcgtcgaca gcgacggggt gttgtaggtc tgctcctggc ggctgttgtc
     9421 cagcgccgtg gacaggttca ggatctccgg cgcccagcgg cccgacaact cctcgatccg
     9481 cgcgatcgcg gccggcgaca gcaccgccag ccacagcccg ccctcgctgg cgaacgcctt
     9541 ctgcggggcg aagtagtagg cgtcgacctc gcgcgggtcg aacgcgatgc cgcccgcgcc
     9601 gctggtcgcg tccaccagca ccagcgcgtc gccggcggga cggcgcagcg gcatcgccac
     9661 gcccgtggag gtctcgttgt gcgtcagcgc gtacgcgtcc acgccgtcgg ccgcctccac
     9721 cgccggatgc gtgccgggct cggactcgaa gatcaccggg tcgccaaggt gcggggccgc
     9781 cttggcggcc ttggcgaact tggacgagaa ctccccgaac acggcgtggg cgctgcgctc
     9841 ccggatgagg tggaacgtgg cggcgtccca gaacagcgtg gagccgccgt tgcccagcgc
     9901 cacctcgtag ccgtccggca gctcgaacag ggcggccagc ccctcgcgca gccgcttgac
     9961 ctgattgcgc accgccggct tgcggtggct ggtgccgagc aggccggggc cggcggccgc
    10021 cagcgcctcg atctgctcgc gccgcacctt ggacgggccg gagccgaagc ggccgtcccg
    10081 gggacgcagc tcggcgggga tgtcgatcgt cgtctcgctc atgggcacac tgtgccagcg
    10141 tcacccctgg tcggccaaag tcagcgcgag gagaatcggt gcatccgttc gcaagccccc
    10201 agggcggggc cgacatgccg ccccccggtc atctgcgcgt gctgggcggc gcccaggacg
    10261 tcgcagtggc cgccgcgaca ccggtggagc tggccggtgc gcgcctgtac cgcctggacg
    10321 acggccgggt gctggcgccc gcgttccgcc gcttcgccgt ggacctgggc gccggcgcgc
    10381 tgccggacga ggccgacgag gacgcgctcg gcgcgctggt gctgcgctgg gtggaggcgg
    10441 tccgggacca cgactggacg ccctaccgcg gcgcggagct ggaaggcggc gaggactggg
    10501 ccgacctgcg cgccgcgatg gtgcggctgg gctggggccc ggacgccgcc gtggcggcct
    10561 gggaggcgtg ccggcagcgc ccctacagcc gcgacctgcc cacggccgcc ggctgggccg
    10621 ccgccgggtg ggtggtcccc gcctcggccg acgagccgga gccggcgttc gcgcgccccg
    10681 tgctggaggc ccgcgggctg taccgctggg gctacatcca gcgctgggcg gcgccggccg
    10741 cgatcccgga gctgccgctg cccgttgagc cggagccggc ggacctcggc tggggcgacg
    10801 tgctgtcgcg gctgcgccag gaccaccaca cccaggactc cctggccgag gccgaacagc
    10861 tcgccatcga gatcctggag ctggcgggcc tcgggccgcg caaccccgtg gaggccgagg
    10921 acgagccgct gccggaccgc gacgtcgccg ccgcgtgcgc gcacgccagc ccgctggtgc
    10981 gcgccgccgc gtggttcctc ctgcgcgacg aggcggaggc gtggctgccg tgcacctaca
    11041 acgagtgcgc gcaccactgg gcggtggagg cggaagagga cgccttcgag atggaggagg
    11101 tgctcgaact ggcttcccgg ggcgtcgcca acccggtcga cgccgcgttc cagcgccgcc
    11161 gcctgctcac cctggcgttc ggcgacatca gcgacgtgcc gctggccggt gcgggcgacc
    11221 tggacgactt catcgccttc tacaccgagt ggaccgcgca cggccagccc gaggaggcgt
    11281 tcgaggagat ggtcgaggag ctgcgcggcg acagcccgct ggacggccct gacgcgagcg
    11341 gctaacgtcg ccaaaacgct gttaccaaac aggagacggg gatcgacatg gcgtcccgca
    11401 cagcccgcgc cctgctcggc ggcacgatcg ccctgctgct gttcaccggg gtcacgctgg
    11461 cgtacgtgcg ccagccggtc gccccggtga gcgccgacac ggcgctggag cgcttccgtt
    11521 cggccgcccc ggagccgcag gcatcgccgg tggcgacgcc ggagcccgga tccagcgcgc
    11581 agccggccga ggccgcgccg tcggagccgg tcgcgaccac cccggacccg gccgggccca
    11641 ccgcggcggc gccggccccc tcccccgctc cgcctccggc cgccggcggc ccggcaccga
    11701 tgccgaaacc gcgcgccggc gtctactcct accggaccac cggctacgag gaggtcgacg
    11761 cgctcggcgg cagccgtcac gactacccgg agaccacgta catgaccatc cgcgacaagg
    11821 ggtgcggcgt ggtgatgcgc tggcagccgc tggaggagcg ctgggacgag agcgacgtct
    11881 gcccggaggg agccgacatc gcgctgcacc gcttcaccac ctaccacgag ttcttccagc
    11941 aggggcaggc acaggcctac gactgcgcgc cggggtcgca cgtgcaccgc ggcggcgcgc
    12001 cgcccggcag cgagtggacg tggcgctgcc agggcgagca cggcgccatc cagacgagcg
    12061 tgcgcgtcgt gggcccggag ccggtcaccg tcggcggcac gaccgtggag gcgatgcgcg
    12121 tgcggtactc gtccgtggcc acggggtcca gccgcggggt gcaggagcag gagcgctggt
    12181 tcgacctgcg gtcgggactg ctggtgcgca tccagaccga tgcgatgctc caggtggaca
    12241 cgcccttcgg gccgaccggc taccgcgagc agtaccgcat cgacctgctc tccctggagc
    12301 cccaaacctg acctgacccg tgtgcgggtg cggtttcaga gcgatggctc tgcaagcgca
    12361 cccgacgacg ggggatcagc ggccgtagcg caggatgcgg tcgtcctcgg ccgcgggctc
    12421 gccgcggcca tcgcggttcg acgtcgtgaa ccacagcgca ccgtcggggg cgctgaccac
    12481 cgcgcgcagc cggccgaact cgccgccgaa cagctcctcc cccggcccca ggccgccctc
    12541 cggcgtgatc gggaagcgcc acagcctccc gccgcgcagc cccgaggcca ccaggctgtc
    12601 gccccactcg ggaaacgcct cgccggccac gaaggccagg cccgaccacg acgcctcgct
    12661 gggctgcttc accgcgagcg cgggacggaa gccgtcggcc cgcgtcgcgc cggtgacctg
    12721 cggccagccg tagttgccgc cgcgcacgat ctggttgacc tcgtcgtcgc ggtccgggcc
    12781 cagctccgcc gcccacatgc gcccgcgccc gtcccaggcc agcccctgca cgttgcggtg
    12841 gcccagggac cacaccggcg agccggggaa cgggttgtcc ggcggcacgg agccgtccgg
    12901 gcgcacgcgc aggatcttgc cggccaggga gccgcgggac tgcgccagct cggaccgccc
    12961 ggcgtcgccc gtgccgacgt acagcatccc gtcgggaccg aaggcgatgc ggccgccgtt
    13021 gtgcacgccc gccttgggga tgccgcgcac cagcacctgc accgggtccg gccggtccag
    13081 cgcgaagcgc accacccggt tgtcctccgc cccggtgaag taggcgtaga ccaggcggtc
    13141 gtcggcgaac tcgggcgaca gggcgatgcc gagcaggccg ccctcgccgc cgggcaccac
    13201 gccgtcgatg ccgcgcaccg ggcggggccg gccctggctg agctcgctga cgacgccggt
    13261 gtcgcgctcc gtgaagaaca cgcggccgtt ggcgctgatc gccaggcccc agggcacctg
    13321 cagctccgtg gcctggatcg tcacggccgg ggacgcgtcc ggttccgcgc cgggccgcgc
    13381 gggcggcgag gccgtcgcgg cggtcccccg ctccggcgcg ccggtcccgt ccggtcccgg
    13441 agggccgccg tcaccggacg cgcagccggc gagcaggacg agcgccagcg cgagcgccgc
    13501 gtacgcaccc gccctactcg tccgtggtgg cctcctgctg ggcaccggcg tccgggaagc
    13561 cctcgctctg cccgatgttg gtgcggggct ccacgctgct ctgcggcggg atgccgccgc
    13621 cggcggcatc ctccggtgag ctgtcaccgc cggagcatcc cgccacggcg gcggccatgg
    13681 cgagcgcgga cacggtggcg ggcagtcggc ggcgcatgtg cgggctccct gggggtcgtg
    13741 gtcctgcccg tatccgtaac cgccgctcat ggccggtaac cctgctcgac gcgcacgagc
    13801 ctggcggcca gcagcgcgcc gacggccggc agcagcgcca ggaccatgag cgcccgcggc
    13861 aggctcacca cttccgacag cccgccgacc gtcgcgctgc ccaccgcgcc gcccacgaag
    13921 aacaccaggt tgaacacgcc cagggcgatg ccgcgcacgt cgtgctccac gccgttggtc
    13981 aggctgtcca gcagggcaac ctgccccccg ccgaagccca tgaagaccag cgccatcccg
    14041 gcgagcaggg ccaccgggac ggcgtggaac agcccgacga cgaccagccc ggccgcgctg
    14101 ccgccgatga ggatcgcggc cacgcgcgag cggccgagcc gcgccgccac cccgccgacg
    14161 gtgcgggacg tgaccgcgcc gacggcggcc ggcaccagca gcaccagccc gatgtgcagc
    14221 ggctcccagc cctggtcgcg cgccaggatc tgcggcacgg cgagcatcgc ccccaggtag
    14281 gccgcgagcg cggtgaggcc cgcgagcccg ttcaggacca ccagcggatt gcgcagcaca
    14341 cggaccggca gcagccccgc cgggcgtgcc cgcacgtgcg ccgccaggaa cgcgaagccg
    14401 gccacggcga cggcggccag gccgagcgcc gggcgcagcc cgatgccggt cgacggcgac
    14461 tgcagcacca tcgtgaacgc cgtgacggcc agtgccacga gcgccgcgcc gcgcacgtcc
    14521 agccgcccgg agcccatcgg ctcgcgcggg gccaggcgcg ccaccggctc ggcgaacagc
    14581 agcgcgatcg ccggcagcgc caggacccag cgccatcccg cgagctgcgc cacacccccg
    14641 ccgatgagcg gcccggcgcc gctgacgatg gacaccacgg cggtgatggc ccccagggcc
    14701 ttgccgcgtg ccgcgtcggt gaagcgggcg gcgatgatgc ccaggccgat cacgggcacc
    14761 gccccggcgc ccatgccctg caggacgcgg cccgccatga gcaccgggaa gctcggcgcc
    14821 gtggcgccca ccagcgagcc ggcggtgaac agcacgatcc cgatgcgcat cggacggcgc
    14881 agcccgtgca ggtccgccag ccggccgaag atcgccgtgg tgatcgcgaa ggacagcgag
    14941 taggccgcga tcacccacgc cgtgcccggc ccgtccaagc ccaggtcctc ccgcaccgtc
    15001 ggcagcgcca cggcgatcgc cgacgagctc accaccgtca aggccacgag cgtgcccagc
    15061 agcagagcca cgcggcgcac cgacgcctca ggggggtcca acgtgtcctc ggtcatcgtt
    15121 cctcccgcgc gccggcgatg ggcgcgaag
//
LOCUS       DATMKC010000052         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_112619, whole genome shotgun sequence.
ACCESSION   DATMKC010000052 DATMKC010000000
VERSION     DATMKC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112619"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1270)
                     /gene="meaB"
                     /locus_tag="VNU01_01470"
     CDS             complement(329..1270)
                     /gene="meaB"
                     /locus_tag="VNU01_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWH31320.1"
                     /translation="MDNRELVDRLLAGERRALARVLTRVEDGSEDKLREIVALLHPRT
                     GSAQLVGITGPPGVGKSTLTSGLIGEWRARGRTVAVLAVDPSSPFSGGALLGDRVRMQ
                     DHVMDAGVYVRSMASRGHLGGLSWATPQALLVLDAAGFDVIIVETVGVGQAEVEIASI
                     ADTTIVALAPGMGDAIQAAKAGILEVADVFCVNKADRDGADRTARELRDMQQLGHGDW
                     IAPIRKTVASSGEGIAELADAVQEHFAWLQDEGRRQRRRLDRARLQVREIALQTVRRR
                     FHEVEGGDLVDELSRRVAAREMDPYTAADKLVSGLHA"
     gene            complement(1333..>2271)
                     /locus_tag="VNU01_01475"
     CDS             complement(1333..>2271)
                     /locus_tag="VNU01_01475"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWH31321.1"
                     /translation="VTVNKVCPSGMTAINLAHQQVLLGEADIVVAGGMESMSNAPYVL
                     PKARTGYRMGNGVLLDSLIHDGLWCAFDNCHMGEGSDAKNAEYAISRQAQDEWSAMSH
                     ERAVTAWKDGKFAGEVTSVEIPQRKGDPVVVDTDEGMRPGTTAESLASLTAAFVKDGT
                     ITAGNASQVSDGGAALIIASRQKAEELGLTPLARILSYGTVAGPDPSLHHQPAAAIEK
                     AAAKIGVEAGGLDLYEMNEAFASVAIHSANLLGLSADKVNVNGGAVALGHPIGCTGAR
                     ITVTLINELRNRGGGRGAASLCGGGGQGDALIVETL"
BASE COUNT          290 a          840 c          816 g          325 t
ORIGIN      
        1 cggcaggacc acggggtcac gaggcggcgc cggcgggatg gtgggcgcgg gctccgggga
       61 cggcggctcc gatgcggcga cctcggtcgc gccggggcgg aacgcggcca cgcccacgcc
      121 gacggtcgcg cccgtcagcg ccagcgccag cgcgccgatg aggaccttgc gggacatccg
      181 catctgctgc gtctttccga tccgtgggga agcgggttcg ggcgttcaga cgcacaggcc
      241 cgccggacgg ttgcccccgg ccgtcccgac ggcacggccg gaagcgcgtt cagggtaacc
      301 gcagaccgcg tgcgcggccg ggcaggcgct acgcgtgcag cccggagacg agcttgtcgg
      361 ccgcggtgta cgggtccatc tcgcgggcgg cgacacgacg ggacagctcg tcgacgaggt
      421 cgccgccctc cacctcgtgg aaccgccggc gcaccgtctg cagcgcgatc tcgcgcacct
      481 gcaggcgcgc gcggtccagg cgccgcctct gtctgcgccc ctcgtcctgc agccaggcga
      541 agtgctcctg cacggcgtcg gccagctccg cgatgccctc gccggaggac gcgacggtct
      601 tgcggatggg ggcgatccag tcgccgtggc cgagctgctg catgtcccga agctcccgcg
      661 cggtgcggtc cgcgccgtcg cggtcggcct tgttcacgca gaacacgtcg gccacttcca
      721 gaatcccggc cttcgccgcc tggatggcgt cgcccatgcc gggggcaagc gccacgatcg
      781 tggtgtcggc gatggaggcg atctccacct ccgcctgccc gacgcccacg gtctccacga
      841 tgatcacgtc gaagcccgcc gcgtccaaca ccagcagcgc ctgtggcgtc gcccagctca
      901 gcccgcccag gtggccgcgg ctggccatcg agcgcacgta cacgcccgcg tccatgacat
      961 ggtcctgcat gcgcacgcgg tcgcccagca gcgcgccgcc cgagaacggc gacgacgggt
     1021 ccacggccag caccgccacg gtcctcccgc gcgcgcgcca ctcaccgatc agcccgctgg
     1081 tcagcgtgga cttgccgacg ccgggcgggc cggtgatgcc gacgagctgg gccgagccgg
     1141 tgcgcgggtg cagcagcgcg acgatctcgc ggagcttgtc ctccgagccg tcctcgacgc
     1201 gcgtgagcac gcgcgccagg gcccgccgct cgccggccag cagccggtcg acgagctcac
     1261 ggttgtccat ccagtggcct tggctacagc gccggtcgcc cgacggctac agggtctcca
     1321 taggctgcat gcctacaggg tctcaacgat gagggcgtcg ccctggccgc caccgccgca
     1381 cagcgacgcc gcgccgcggc cgccgccgcg attgcgcagc tcgttgatga gcgtgacggt
     1441 gatgcgcgcg ccggtgcagc cgatcgggtg gccgagcgcc accgcgccgc cgttcacgtt
     1501 caccttgtcg gccgagaggc cgagcaggtt ggcggaatgg atggcgaccg aggcgaacgc
     1561 ctcgttcatc tcgtacaggt ccaggccgcc cgcctcgacg ccgatcttgg ccgcggcctt
     1621 ctcgatcgcc gccgccggct ggtggtgcag ggacgggtcg gggccggcca cggtgccgta
     1681 ggacaggatg cgcgcgagcg gcgtcaggcc cagctcttcg gccttctggc gggacgcgat
     1741 gatcagcgcc gcgccgccgt cggagacctg gctggcgttg ccggccgtga tggtgccgtc
     1801 cttgacgaac gcggcggtga ggctggccag gctctcggcc gtggtgccgg gacgcatgcc
     1861 ctcgtcggtg tcgaccacga ccgggtcgcc cttgcgctgc gggatctcga cgctcgtgac
     1921 ctcgcccgcg aacttgccgt ccttccaggc ggtcacggcg cgctcgtgcg acatcgccga
     1981 ccactcgtcc tgcgcctggc ggctgatcgc gtactcggcg ttcttcgcgt cgctgccctc
     2041 gcccatgtgg cagttgtcga aggcgcacca caggccgtcg tggatcaagg agtccaggag
     2101 cacgccgttg cccatgcggt agccggtgcg cgccttgggc agcacgtagg gcgcgttgga
     2161 catggactcc atgccgcccg cgaccacgat gtcggcctcg ccgagcagga cctgctggtg
     2221 cgccaggttg atcgccgtca tgccggaggg gcagaccttg ttcaccgtga c
//
LOCUS       DATMKC010000053         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_112440, whole genome shotgun sequence.
ACCESSION   DATMKC010000053 DATMKC010000000
VERSION     DATMKC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112440"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..543)
                     /locus_tag="VNU01_01480"
     CDS             complement(121..543)
                     /locus_tag="VNU01_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWH31322.1"
                     /translation="MAKPADTKTAAQPTGAARSSRSQADTEQIRAALAARRDELHAEY
                     EQTLTEITEMQRDRLTDSAGDDQADTGTKTFEREQEISLANNILERITQVERALERLD
                     EGGYGWCERCGNAIPVERLAAFPSATLCVSCKQLEERR"
     gene            611..928
                     /locus_tag="VNU01_01485"
     CDS             611..928
                     /locus_tag="VNU01_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31323.1"
                     /translation="MRGAVPPVGADPADPAGAVGLAVLDLAVLVLAAVALAVPVLADV
                     ALAVPVLADVALAVADVAAAAPVSALAADRSVAGSPDAPCSPAAAPDPPRGGVAAFFP
                     GVA"
     gene            complement(1169..>2054)
                     /locus_tag="VNU01_01490"
     CDS             complement(1169..>2054)
                     /locus_tag="VNU01_01490"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HWH31324.1"
                     /translation="RWTLRRAALPSAGLYPIAAVALTVLAYAAGAVAHASGFLAVYVA
                     GVLLGNARLPHRHAILGFAEGLAWVAQIGLFVMLGLLVAPSRLGAAVLPALLVGAVLL
                     LLARPASVVASALPFRVTVREQAFLSWAGLRGAVPIVLATIPLSAAVPGAAGLFDTVF
                     VLVVVFTLVQVGTLRPVAWGLRVTAPGEAAELLVETAPLERMRADLLQVDVPVGSRLA
                     GVHLDELRLPAGAVVTLVLRDGVGFVPGRNTRLRTGDSLLIVATADVREATESRLRAV
                     SRRGRLARWLDGRGDQGP"
BASE COUNT          253 a          814 c          731 g          256 t
ORIGIN      
        1 gccatgcccg cctgcgatcg gcgcgcgctg gtgccggcgc tgccgccgcg ctacccgggt
       61 cgtccaccac tgtcctcccc gcggcggtcg cgacgcgggc cctgcgcccg cgcccacgtt
      121 tcagcgccgc tcctccaact gcttgcacga gacacacagt gtggcggacg ggaacgcggc
      181 caggcgctcc acggggatgg cattgccgca ccgctcacac cagccgtagc cgccctcgtc
      241 gagtcgctcc agggcccgct ccacctgggt gatgcgctcc aggatgttgt tcgccagaga
      301 gatctcctgc tcccgctcga acgtcttggt gccggtgtcg gcctggtcgt cgccggccga
      361 gtcggtcagc cggtcgcgct gcatctccgt gatctcggtg agcgtctgct cgtactcggc
      421 gtgcagctcg tcgcggcggg ccgccagcgc cgcccggatc tgctccgtgt ccgcctggct
      481 gcggctggac ctcgcggccc cggtcggctg ggcggcggtc ttggtgtcgg ctggcttggc
      541 catcttcgct ccctcggccg cgggcgtcgc ggcggtcggc cgtgcctggt cccgacccac
      601 ggacgccggc gtgcggggcg ctgtgccccc ggtcggcgcc gaccccgcgg acccggccgg
      661 agcagtcggc ttggcggtgc tggacttggc ggtgctggtc ttggcggcgg tcgccttggc
      721 ggtgccggtc ctggcggacg tcgccttggc cgtgccggtc ctggcggacg tcgccttggc
      781 cgtcgccgac gtcgcggccg cggccccggt gtccgccctg gcggcggacc ggtccgtcgc
      841 cgggtctccg gacgcgccgt gctcgcccgc tgcggccccg gacccgccgc gcggcggggt
      901 ggccgccttc ttccccggcg tggcctgagc cgccgccggg tggtcgccgg tggtccgctt
      961 cgccgacgcg cgcgcccccg cggcgttacg cgcagcgtct gaccgctcgg ccatcgcgtc
     1021 ccccagctgc aaaaacggcg cgcggcagga cgccacgcac tccgaggttg gcaagaatac
     1081 ggaacgtaca cgcgtcccga caacctggcg caccgttcgt actgaattcg gcctttctgc
     1141 ccctgccgtt caggcgggca tcccgccgtc acggaccctg atcgccccgc ccgtccaacc
     1201 accgagccag ccgcccccga cggctcacgg cccgcagacg gctttcggtg gcctcgcgga
     1261 cgtccgcggt ggccacgatc agcaggctgt cgccggtacg cagcctggtg ttgcgcccgg
     1321 gcacgaagcc caccccgtcg cgcaggacca gggtgacgac cgcacccgcc ggcaaccgca
     1381 gctcgtccag gtgcaccccg gcgagccggg aacccaccgg cacgtcgacc tggagcaggt
     1441 cggcgcgcat gcgctccagc ggcgcggtct ccaccagcag ctcggcggcc tcacccggcg
     1501 cggtcacccg cagcccccac gcaaccggcc gcagcgtgcc cacctgcacc agggtgaaca
     1561 ccacgaccag cacgaagacg gtgtcgaaca ggcccgccgc ccccggaacg gccgcggaga
     1621 gcggaatggt ggccagcacg atcggcaccg cgccccgcag cccggcccag gacaggaacg
     1681 cctgctcccg aacggtcacc cggaacggca gcgcgctggc cacgaccgac gcggggcggg
     1741 cgaggaggag caggaccgca ccgaccagca gcgccggcag cacggcggcg cccaggcggg
     1801 acggcgccac caggagcccg agcatcacga acagtccgat ctgcgcgacc caggccaggc
     1861 cctcggcgaa gccgagaatg gcgtgccggt gcggcagccg cgcgttgccc agcagaacgc
     1921 cggcgacgta cacggccagg aatccggagg cgtgcgcgac cgccccggcg gcgtacgcga
     1981 gtacggtcaa cgccaccgcc gcgatcggat agagccccgc ggacggcagg gcggcgcggc
     2041 gcagcgtcca gcgg
//
LOCUS       DATMKC010000054         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_46974, whole genome shotgun sequence.
ACCESSION   DATMKC010000054 DATMKC010000000
VERSION     DATMKC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46974"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="VNU01_01495"
     CDS             complement(<1..1765)
                     /locus_tag="VNU01_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31325.1"
                     /translation="MRRARPAVPVIAAFFLLVAFAVPAAADHTDPTSPLAPTDGIAAD
                     GRATGAGAWEHLQHFPPNPGSDLWFFEKDGATYASGGTLGQGDEGHVGQRIVQLLDGD
                     GELDVRWVADHGSANCPTANPAGTLGLQHDVEPVPLTEPELLVDATDATGRCHDPAGG
                     GIEIVDVSGLGEEGFEVRELHLVRFPGTTHTLTVDQERPWIVYSNNSDSSNRNWVDFM
                     DLRSCLTAASGGTMPEGLSLEDKRAHCAPDVYRIPFEDHWTQQTTTEDGEPSGGSASC
                     HDSTSVGDRLYCSGLKGEVILDVAGLVDGDGDLHPDSVPLACTTADGTLTGANVTNCA
                     MHTSKGSAFAAGWELVGFFNHVGTTAGNTNHNVSADRGVAVSHQSVPTPQEVGDYLIV
                     SDERGGGVVPPGASCAPGIDNPYGNGGLHFFDISDPAAIAHAPLSGTGGEQPAVWIGD
                     VIVPAPSFCTVHVFHHVPDEQRLIMGYYSQGIKVLDYHVEEDGLRFEEIASYVLPGAN
                     TWVANFFKITDNADGTRTYHLMTNDIQRGMDVVSWTGPTNPIPGDDDGRGWLPRSRSA
                     WRSQSGPPRPSSSPGIGLVGPV"
     gene            1790..>2474
                     /locus_tag="VNU01_01500"
     CDS             1790..>2474
                     /locus_tag="VNU01_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31326.1"
                     /translation="MADFGPFGHPVPLLSGLHPLDSVSSALPPAGVLALWALLAVGAG
                     GWLGARLGQSRPAADVPAHHAAEPLGPWRAAGRVAGVALLALAIVAGRLGNPRELSNI
                     APALVVGVAWPALLAGSALFGRVWAVLDPWDGIARVLRAPAGEAEPGDVWPAAAVALL
                     WTWYLAVPFDALSPRNVGLALALYSLVTVAGCLVAGRAGWLARAELFGLLSRWLALWR
                     RGATVPASAK"
BASE COUNT          284 a          863 c          954 g          373 t
ORIGIN      
        1 ggaccgggcc gaccaacccg atccccggcg acgacgacgg ccgcggcggg ccggactgcg
       61 agcgccacgc cgagcgcgag cgcggcaacc agccgcggcc gtcgtcgtcg ccggggatcg
      121 ggttggtcgg cccggtccag ctcaccacgt ccatgccgcg ctggatgtcg ttcgtcatca
      181 ggtggtaggt gcgcgtgccg tcggcgttgt cggtgatctt gaagaagttc gccacccagg
      241 tgttcgcgcc cggcagcacg taggaggcga tctcctcgaa gcgcagcccg tcctcttcca
      301 cgtggtagtc gagcaccttg atgccctgcg agtagtagcc catgatcagc cgctgctcgt
      361 ccggcacgtg gtggaacacg tggacggtgc agaacgacgg cgcgggcacg atcacgtcgc
      421 cgatccacac cgccggctgc tcgccgccgg tccccgacag cggcgcgtgc gcgatggcgg
      481 ccggatccga gatgtcgaag aagtgcaggc cgccgttgcc gtacgggttg tcgatgcccg
      541 gcgcgcacga cgcgcccggc ggcaccacgc cgccgccgcg ctcgtcggac acgatcaggt
      601 agtcgcccac ctcctggggc gtcgggacgc tctgatgcga gaccgcgacg ccccggtcgg
      661 cggacacgtt gtggttggtg ttgccggcgg tggtgccgac gtggttgaag aagccgacca
      721 gctcccagcc ggccgcgaag gccgagccct tgctggtgtg catcgcgcag ttggtgacgt
      781 tcgcgccggt cagcgtgccg tcggcggtgg tgcacgccag cggcaccgag tccggatgga
      841 ggtcgccgtc cccgtccacc aggccggcga cgtcgaggat cacttcgccc ttcagccccg
      901 agcagtacag gcggtcgccg acggacgtgg agtcgtggca gctcgccgag ccgccggagg
      961 gctccccgtc ctcggtggtg gtctgctgcg tccagtggtc ctcgaacggg atgcggtaga
     1021 cgtccggcgc gcagtgcgcg cgcttgtcct ccaggctcaa gccctccggc atcgtgccgc
     1081 ccgacgcggc cgtgaggcag ctgcgcaggt ccatgaagtc gacccagttg cggttggagg
     1141 agtcggagtt gttgctgtag acgatccagg ggcgctcctg gtccaccgtc agcgtgtggg
     1201 tggtgccggg gaagcgcacc aggtgcagct cgcggacctc gaagccctcc tcgcccagcc
     1261 cgctgacgtc gacgatctcg atgccgccgc ccgccgggtc gtggcagcgg ccggtggcgt
     1321 cggtcgcgtc caccaggagc tccggctccg tcagcggcac cggctcaacg tcgtgctgca
     1381 ggcccagcgt gccggccggg ttggcggtcg ggcagttcgc ggagccgtgg tcggcgaccc
     1441 agcgcacgtc cagttcgccg tcgccgtcca ggagctggac gatgcgctgg ccgacgtggc
     1501 cctcgtcgcc ctggcccagc gtcccgccgg aggcgtaggt cgcgccatct ttctcgaaga
     1561 accacaggtc acttcccggg ttcgggggga agtgttggag gtgctcccag gcgcccgcgc
     1621 cggtggcccg cccgtccgcg gcgatcccgt cggtgggcgc gaggggagac gtgggatcgg
     1681 tgtggtccgc ggccgccgga acggcgaacg cgacgagcag gaagaaggcg gcgatgaccg
     1741 gaacggccgg gcgagctctg cgcatgggcg accctcgatg acggctggcg tggccgattt
     1801 cggcccattc gggcacccgg tacccctgct gtcagggctt cacccgctgg attccgtaag
     1861 ttcggcgctg ccgccggcgg gcgtcctggc gctgtgggcg ctgctcgccg tgggcgccgg
     1921 cgggtggctc ggcgccaggc tgggccagtc acgcccggcc gccgacgtgc cggcgcacca
     1981 cgccgccgag ccgctgggcc cgtggcgcgc cgccggccgc gtggccggcg tggcgctgct
     2041 cgccctggct atcgtggccg ggcggctggg caacccccgg gagctgtcca acatcgcccc
     2101 ggccctggtg gtgggcgtgg cgtggccggc gctgctggcg gggtccgcgc tgttcgggcg
     2161 ggtgtgggcg gtgctggacc catgggacgg catcgcgcgc gtgctgcgcg cgccggcggg
     2221 cgaggcggag ccgggcgacg tctggccggc ggccgcggtg gcgctgctgt ggacgtggta
     2281 cctcgcggtg ccgttcgacg cgctgtcacc gcgcaacgtc ggcctcgcgc tggcgctgta
     2341 ctcgctggtc accgtggccg gctgcctggt ggccgggcgt gccggctggc tggcgcgcgc
     2401 cgagctgttc ggcctgcttt cgcgctggct ggcactgtgg cgccgcggcg ccacagtgcc
     2461 agccagcgcg aaag
//
LOCUS       DATMKC010000055        12153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_112238, whole genome shotgun sequence.
ACCESSION   DATMKC010000055 DATMKC010000000
VERSION     DATMKC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112238"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VNU01_01505"
     CDS             complement(<1..856)
                     /locus_tag="VNU01_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWH31327.1"
                     /translation="MRWDLPPLVAGFALGLAAVLALGAAQPSAQRAAAGAQLPSARPA
                     PADPGCQVAAERLAGEVLVAGLPGVLAPNHPLVADLVNLQVGGVLIEKSNVRNAAQVR
                     RLVGAMRDGSDRPLLVTTDEETGRVSNFGEVLGRFSAARTLAKRGSPADVERFARQLG
                     VELANLGMDVVFAPVADVDDGPATGMIGDRSFSGDPEEAGAYALAYARGLWSGGVLPA
                     PKHFPGAGRARAPAAGELPRVDAAPGELRGHDLGPFRAQIRAGVRMLMVGNVVVEAVD
                     PQQPVRGRR"
     gene            complement(905..1675)
                     /locus_tag="VNU01_01510"
     CDS             complement(905..1675)
                     /locus_tag="VNU01_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWH31328.1"
                     /translation="MAATVGTTGERAPGLYRFLHGIIPPVLNALYRPWTEGMEHIPSS
                     GPAILASNHLSFLDSIFLPAMLERPVFFLGKSDYFQGWKRWFFEGVGVMPVHRQGGDA
                     GEASLRKGQEILESGRLLGIYPEGTRSPDGRLYRGKTGPVRLALRSGAPIIPVAMIGT
                     FEILPPGANIPKIRRVGVRVGRPLDFSRYAGMENDRFVLRSATDELMYELMMLSGQEY
                     VDEYAAKVKSGEVIIGSGDLEELAALIGEDERLLSKAS"
     gene            1877..2092
                     /locus_tag="VNU01_01515"
     CDS             1877..2092
                     /locus_tag="VNU01_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31329.1"
                     /translation="MDPFDEPGPDSDEDGPPLVMERLRKFGWVAVVLAPLLVITLAQI
                     SIGVEWAIIVFVGGLVAITAWRQREDG"
     gene            complement(2096..3052)
                     /locus_tag="VNU01_01520"
     CDS             complement(2096..3052)
                     /locus_tag="VNU01_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWH31330.1"
                     /translation="MRGAPAPAAAVGVDVGGTKTVGGVVGADGTVRVRARVPTPPQGG
                     DAVTDAIAALVADLWRQAGGAPLPVGVGAAGVFDRSGVLRYSPNIPGWDEVPLAARLS
                     EALGVAVRADNDANVAAWGECRAGAARDIEGTVVMLTVGTGIGGGLVQDGALVRGAHG
                     MAAEFGHIVVAEGGPLCGCGNRGCLEALASGTAIGRTAAQRLAAGHGSALAGEGSAPT
                     GKSVTLAAQAGDELATAVLAECGFWLGVGIASLVNALDPDLVVVGGGAMQAGEMLLGP
                     AREAAAARVIGGAHRQPTPVVAAALGDDAGLVGAALLALEAG"
     gene            complement(3053..4171)
                     /locus_tag="VNU01_01525"
     CDS             complement(3053..4171)
                     /locus_tag="VNU01_01525"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HWH31331.1"
                     /translation="MDVTAADIRKLVDRPVGRVSVTSVYLNTDGARFPKAADYEARLD
                     ALLRDVRKAADKLDVRRREGVHADVEEISRWVRREFERGDTRGLALFACGGEIFETVQ
                     IAEGFRNVARVGDRPYVVPLEAMLGRYHHIGLALVQRDKAMILRYQLGRLWTWQGLVS
                     DVHGQHVQGGWSQRRFELGIEHEVLHHFKDTAEVLRLLHEEQPMDALVLAGPHEEVVD
                     FQRQLHPYLQKIVHGEPQNLDAYADRNTILERLRAVEQELVSARRAELLERLAAAQGQ
                     AEKAARGLRHVLEAVNGKRVETLFVVEGTGVPGWRSANGALALHEDEAAAFGTPVEPV
                     DDVVDEVIEEAVRSGAHIEFFRDEVRLDGHPVAALLRF"
     gene            complement(4246..4527)
                     /locus_tag="VNU01_01530"
     CDS             complement(4246..4527)
                     /locus_tag="VNU01_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31332.1"
                     /translation="MSDSDGNGQDHPPPDGHAHTLPGGVGGSQECLLCPVCVFLQAMT
                     QARPEVTQHLLAAARELTLALRAVVETQAEAYEQAAQERSARFERIKVD"
     gene            complement(4539..5732)
                     /locus_tag="VNU01_01535"
     CDS             complement(4539..5732)
                     /locus_tag="VNU01_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HWH31333.1"
                     /translation="MRVLLFTGKGGVGKTSVAAASALRAAAAGLRTLVVSTDPAHSLA
                     DALDVPLGDRPVSVAPRLDAEQIDAQARLEEHWRDIQGYLIGLLQWGGVGEIEAEELS
                     VIPGLEEIFALVDVRDHVEAGNYDLLVVDCAPTAETLRLLSLPDALGWYMERVFPAGR
                     TLARMSRPVLSRVTTMPLPQDKVLGSVEDLYRRLAGVRDILSDGSRSTVRLVVNAERM
                     VIAEARRTMTALSLFGYSVDAVVVNRLIPATVTDPWFDRWKAIQAEHLAAVRDGFAGV
                     PVLTAPLFDAEPIGAEALGRLAAELYGEQAVEAVLHEGTPLTFEREGEDWLLRLALPF
                     ADKADLDLFHRGAELTVKVGSHVRKVALPAALGRSDVAGAALRDGRLEVRFSPRAAQP
                     QRAST"
     gene            complement(5740..6177)
                     /locus_tag="VNU01_01540"
     CDS             complement(5740..6177)
                     /locus_tag="VNU01_01540"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH31334.1"
                     /translation="MSETVRESILVKADADTIMDVIADFDAYPDWQPDFKSVEVLETD
                     DDGWATKVRFVLDAMIMTTSYTLAYTYTDTSMSWHLVEGEQVRKVDGSYTLEDAGGGQ
                     TKVTYQLEVDPTISVPGILKRKATKKIVDSALKGMKQRVESLA"
     gene            complement(6243..6821)
                     /locus_tag="VNU01_01545"
     CDS             complement(6243..6821)
                     /locus_tag="VNU01_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31335.1"
                     /translation="MRIETWDTGFVRARRRDVHALLALPLDRYGQWWPGVRARGAGAD
                     RVELAFRPPGLLRRSHRVTVEIVKRRPNLGVSYRFTGRALTGEAEFFYTDEPNGVVVN
                     YLLRVETRGGARRARRLTRDHRAVAREALHALKDRCEGGRLPGTDPDPRLLADQRDAI
                     AEFQAGVEAHARKVAAAQAGQAAEQGGGVAAP"
     gene            complement(6943..7755)
                     /locus_tag="VNU01_01550"
     CDS             complement(6943..7755)
                     /locus_tag="VNU01_01550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWH31336.1"
                     /translation="MEAERTIPVCYRHPDRETRLACTHCDRPVCWDCVTPAPVGQRCP
                     ECARDTGPRIRAVRRDTAPVSMGVIGVAAAVFVLGLLAPAVGRELFLWFGQHNASVLA
                     GDSYRVVTAGFLHAGFTHILFNMWALSAFGPPLEREAGSTAFGGMYLASLLCGGLAYL
                     WLGEPGGFAVGASGAIFGLFGAWTAIAVANRHTVMGQAQLRQMLMLLGINAALPILVP
                     QIAWQAHLGGFLAGFLLGMVWSAVRGQERATVLRAASGYGLAALAWLALLLT"
     gene            8017..9240
                     /locus_tag="VNU01_01555"
     CDS             8017..9240
                     /locus_tag="VNU01_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWH31337.1"
                     /translation="MIFVGIDWSERHHDVEVQRDDGTTVERARVGEGLEGAARLHALL
                     AAHAEDPGEVVIGIETDRGLLVGSLAAAGYQVFAVNPKAVSRYRDRHAMSGAKSDARD
                     AKVLADLVRTDRHNHRPIAGDSDLAAAIKVTARAHQNLIWTRQRQLNQLRNTLRDYYP
                     AALEAFGTDLAHPDAVAVLTKAPTPERGRRLSTAQIASALRRAGRKRNIDSRAAAIRD
                     ALRTPQLSAPPAVADAYGDTTKALFGVIGALTAQISELEAALTDRFEQHPDADIYRSL
                     AGLGVILGARVLAEFGDDPDRYADAKARRNYAGSSPITKASGTRRVVLARYVRNRRLA
                     DALTWWAFCSLTTSPGARAFYDRHRANGHTHHQALRALSNRWVGILHGCLRHHTPYDE
                     TAAWRHRNEQLPTAA"
     gene            complement(9262..10071)
                     /locus_tag="VNU01_01560"
     CDS             complement(9262..10071)
                     /locus_tag="VNU01_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWH31338.1"
                     /translation="MSRARRLLHRAARGAARSLSPDLRELALALGARAAAGPAVLPGP
                     PEGPVLVVAPHPDDETIGCGGALARHARRGDAVTVLVATSGEATRGGSGDVAAAREAE
                     CRAACADLGIGEPVFLRLPDGALAGHAGELAEAVARHGAGARTVYLPSLLDPHADHRA
                     ANAAVAAAGLDADTYGYEVWAAAPVDALLDVTAVWARKETALRRYATALESVDYVRVC
                     AGLAAYRSGAGGLDGAGMAEGFLRLAAEEHRRLAAGVGLARGLGGASEEDG"
     gene            complement(10068..11864)
                     /locus_tag="VNU01_01565"
     CDS             complement(10068..11864)
                     /locus_tag="VNU01_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HWH31339.1"
                     /translation="MAITHVKGRTPVEVAPDQNLTTRLWERAATSTGREILRHWTNGS
                     WRGLTWKQLGDRVAAVGAGLIAAGIEPGDRVALMSGTCLDWTIADLAILSVGGVTVPI
                     YETSSVEQCAWILSDSGAKLAFAQKAEHAKNLDEAREQAKNLSEIFVFADAGLDALAE
                     RGDDAGRAQVLERAKKVKAADLASIIYTSGTTGNPKGCMLTHHNLVWTARQTEQNLRE
                     LFGGEESTLLFLPLAHVFARLIQFGCLESDVTLGYSRGIDQLQEDLVSFSPTFLLSVP
                     RVFEKVFNGAQRKAEGPKRKVFDFAVQTAYAWSEAKNEGRNPGLGVNVKHAVADKLVY
                     SKLRDALGGRVRYCVSGGAPLAPHLAHFFHAAGITILEGYGLTETSAPATANSPQAFK
                     IGTVGQPLPGVEIKIAEDGEILIKGGNVFTGYYKNDAASSEVLEDDGWFHSGDIGELD
                     DQGYLAITGRKKEIIVTAGGKNVAPAVLEERIKANRLVSQAMVVGDNRPFVACLITLE
                     PEELAAFAKEHGLSGTTVAQLKDDPAVLAEVQKAIDHGNAAVSKAEAIRKFAILERDF
                     TIEDDELTPSLKVRRKNVVDHFGEAIEGLYQK"
BASE COUNT         1615 a         4583 c         4311 g         1644 t
ORIGIN      
        1 ggcgccggcc gcgtacgggc tgctgcgggt cgacggcctc gaccaccacg ttgccgacca
       61 tcagcatgcg cacgccggcg cggatctgcg cgcggaaggg gccgaggtcg tggccgcgca
      121 gctccccggg cgcggcgtcc acgcggggca gctcgccggc ggcgggggcg cgcgcccggc
      181 cggcgcccgg gaagtgcttg ggggccggca ggacgccgcc gctccacagc ccccgcgcgt
      241 aggcgagcgc gtacgcgccg gcctcctcgg ggtccccgga gaacgagcgg tcgccgatca
      301 tgcccgtcgc ggggccgtcg tcgacgtccg ccaccggggc gaacaccacg tccatgccga
      361 ggttggccag ctccacgccg agctgccggg cgaagcgttc gacgtcggcg gggctgccgc
      421 gcttggccag cgtgcgcgcc gcggagaagc ggcccagcac ctcgccgaag ttggacaccc
      481 tgccggtttc ctcgtcggtg gtcaccagca gcggccggtc gctgccgtcg cgcatggcgc
      541 cgacgaggcg gcgcacctgc gcggcgttgc ggacgttgct cttctcgatc agcacgccgc
      601 cgacctgcag gttcaccagg tcggccacca gcggatggtt cggcgccagc acgcccggga
      661 gcccggcgac caggacctcg cccgccagcc gctccgccgc gacctggcag cccgggtcgg
      721 ccggggccgg cctggcggag ggcagttgcg ccccggcggc ggcccgctgc gccgacggct
      781 gcgccgcgcc gagcgcgagc acggcggcga ggcccagcgc gaagccggcc acaagggggg
      841 gaaggtccca gcgcacgacg cctgagcgta cccgcgtccg cgcccggcgc ggaatcggcc
      901 gcgcctagct cgccttggac agcagccgct cgtcctcgcc gatgagcgcc gccagctcct
      961 cgaggtcgcc ggagccgatg atgacctcgc cggacttcac cttggcggcg tactcgtcca
     1021 cgtactcctg gccggacagc atcatcagct cgtacatcag ctcgtccgtg gccgagcgca
     1081 gcacgaagcg gtcgttctcc atcccggcgt agcgggagaa gtccagcggc cggcccacgc
     1141 ggacgccgac ccggcggatc ttggggatgt tggcgcccgg cggcaggatc tcgaaggtcc
     1201 cgatcatggc gaccgggatg atcggcgcgc cgctgcgcag ggccagccgc accggcccgg
     1261 tcttgccgcg gtacagccgg ccgtccgggg agcgcgtgcc ctccgggtag atgcccagca
     1321 ggcggcccga ctccaggatc tcctggccct tgcgcaggga cgcctcgccg gcgtcgccgc
     1381 cctggcggtg caccggcatc acgccgacgc cttcgaagaa ccagcgcttc cagccctgga
     1441 agtagtcgct cttgcccagg aagaacaccg ggcgctccag catcgccggc aggaagatgg
     1501 agtccaggaa cgacaggtgg ttggacgcca gaatggccgg cccgctggag gggatgtgct
     1561 ccatgccctc cgtccacggc cggtacagcg cgttgagcac cggcgggatg atcccatgga
     1621 ggaaccggta caaccctggt gcccgctccc ctgtcgtgcc cactgtggcc gccacccctc
     1681 ctcgctcatc ccgccaatgg cggagtctac gcctctgccc ggaatgttct accgcaacgt
     1741 cgtcacaggg gtgactgccc cacgggacaa ggcgcgatgc ggcccggcgc gtgaagaacc
     1801 atccgaaaac ggtggtttcc ggtccaacca gccgctgcgg ccgctgggca cggcgccggc
     1861 gtggcaggct tgacggatgg accccttcga cgagccaggc ccggacagcg acgaggacgg
     1921 cccgccgctc gtgatggagc ggctgcgcaa gttcggctgg gtcgcggtcg tgctggcccc
     1981 gctgctggtg atcacgctgg cgcagatctc catcggcgtg gagtgggcca tcatcgtctt
     2041 cgtcggcggg ctcgtcgcca tcacggcctg gcgccagcgc gaggacggct gacgctcagc
     2101 cggcctcgag cgccagcagg gcggcgccga cgaggccggc gtcgtcgccc agcgccgcgg
     2161 cgaccaccgg cgtgggctgc cggtgcgccc cgccgatgac gcgcgccgcg gccgcctccc
     2221 gcgccggccc cagcagcatc tcgcccgcct gcatcgcgcc gccgcccacc accacgaggt
     2281 ccgggtccaa cgcgttcacc agcgacgcga tgccgacgcc cagccagaac ccgcactcgg
     2341 ccagcaccgc ggtcgccagc tcgtcgccgg cctgggccgc cagcgtcacg gacttgccgg
     2401 tcggcgcgct cccctcgccg gcgagcgcgg acccgtggcc cgcggccagc cgctgcgccg
     2461 ccgtgcgccc gatcgcggtg ccgctggcca gcgcctccag gcagccgcgg ttgccgcagc
     2521 cgcacagcgg accgccctcg gcgaccacga tgtggccgaa ctcggcggcc atgccgtgcg
     2581 cgccgcgcac cagcgcgccg tcctggacca gccccccgcc gatgcccgtg ccgacggtga
     2641 gcatcaccac ggtcccctcg atgtcgcgcg ccgcaccggc gcggcactcg ccccaggcgg
     2701 cgacgttggc gtcgttgtcg gcgcgcacgg ccacgcccag cgcctcgctc aggcgcgccg
     2761 cgagcggcac ctcgtcccag ccggggatgt tcggcgagta gcgcagcacg ccggagcggt
     2821 cgaacacgcc ggccgcgccg acccccaccg gcaggggcgc cccgcccgcc tggcgccaca
     2881 ggtcggcgac cagcgccgcg atggcgtcgg tgaccgcgtc gccgccctgc ggcggcgtcg
     2941 gcacgcgcgc gcgcacgcgg accgtgccgt cggcgccgac cacgccgccc accgtcttgg
     3001 tgccgccgac gtcgacgccg acggcggcgg ccggcgcggg ggcgccgcgc aatcagaacc
     3061 tcagcagcgc ggcgacgggg tggccgtcca ggcgcacctc gtcgcggaag aactcgatgt
     3121 gggcgccgct gcgcacggcc tcctcgatca cctcgtccac cacgtcgtcg accggctcga
     3181 ccggggtgcc gaacgcggcg gcctcgtcct cgtgcagggc cagggcgccg ttggcgctgc
     3241 gccagcccgg cacgccggtg ccctccacca cgaacagcgt ctcgacccgc ttgccgttga
     3301 ccgcctccag cacgtggcgc aggccgcgcg cggccttctc cgcctggccc tgggcggcgg
     3361 ccagccgctc cagcagctcg gcgcgccggg cgctcaccag ctcctgctcc accgcccgca
     3421 gccgctccag gatggtgttg cggtcggcgt aggcgtcgag gttctgcggc tcgccgtgga
     3481 cgatcttctg caggtacggg tggagctggc gctggaagtc gaccacttcc tcgtgcgggc
     3541 cggccagcac cagggcgtcc atcggctgct cctcgtgcag caggcgcagc acctccgcgg
     3601 tgtccttgaa gtggtgcagg acctcgtgct cgatgcccag ctcgaagcgc cgctgcgacc
     3661 agccgccctg gacgtgctgg ccgtgcacgt cgctcaccag gccctgccac gtccacagcc
     3721 gcccgagctg gtagcgcagg atcatcgcct tgtcgcgctg gaccagcgcc aggccgatgt
     3781 ggtggtagcg gcccagcatg gcctccagcg gcaccacgta cgggcggtcg cccacgcggg
     3841 cgacgttgcg gaagccctcg gcgatctgca ccgtctcgaa gatctccccg ccgcacgcga
     3901 acagcgccag cccgcgcgtg tcgccgcgct cgaactcgcg gcgcacccag cgggagatct
     3961 cctcgacgtc ggcgtggacg ccttcgcgcc gccgcacgtc cagcttgtcg gcggccttgc
     4021 ggacgtcgcg cagcagcgcg tccaggcggg cctcgtagtc cgccgccttg gggaagcgtg
     4081 ccccgtcggt gttgaggtag acgctggtga ccgagactct gcccaccggc cggtcgacga
     4141 gcttgcggat gtcggcggcg gtgacgtcca tccttcctcc tgtcgtcttc gcggcgcgcc
     4201 ctgctggggg cgcacgctag cccatcggcc gcagtcctgc cccggtcagt ccaccttgat
     4261 gcgttcgaag cgggcggagc gctcctgggc ggcctgctcg taggcctcgg cctgggtctc
     4321 caccaccgcg cgcagcgcca gagtgagttc gcgggccgcg gccagcaggt gctgggtgac
     4381 ctccggcctg gcctgcgtca tggcctgcag gaacacgcac acggggcaca gcaggcactc
     4441 ctgactgccg ccgacgccgc cgggcagcgt gtgggcgtgc ccgtccggcg gcggatggtc
     4501 ctggccgttg ccgtcactgt cgctcatgtg gcccgagtct aggtgctcgc ccgctggggc
     4561 tgcgccgccc gggggctgaa gcgcacttcc aggcgcccgt cgcgcagcgc ggcgccggcg
     4621 acgtcgctgc gcccgagcgc cgccggcagc gcgaccttgc gcacgtgcga gccgaccttg
     4681 acggtcagct ccgcgccgcg gtggaacagg tccaggtcgg ccttgtcggc gaacggcagc
     4741 gccaggcgca gcagccagtc ctcgccctcg cgctcgaacg tcagcggcgt gccctcgtgc
     4801 aggacggcct ccaccgcctg ctcgccgtac agctcggcgg ccaggcggcc gagcgcctcg
     4861 gcgccgatcg gctcggcgtc gaacagcggc gcggtcagca ccggcacgcc cgcgaagccg
     4921 tcgcgcaccg ccgccaggtg ctcggcctgg atcgccttcc agcggtcgaa ccacgggtcg
     4981 gtgaccgtgg cggggatgag gcggttcacc accacggcgt ccaccgagta gccgaacagg
     5041 ctcagggccg tcatggtgcg ccgcgcctcc gcgatcacca tgcgctcggc gttgaccacc
     5101 aggcgcacgg tggagcggct gccgtcggac aggatgtcgc gcacgccggc cagccggcgg
     5161 tacaggtcct ccacggagcc cagcaccttg tcctgcggca gcggcatggt ggtcacgcgg
     5221 ctgagcaccg gccggctcat gcgcgccagc gtgcgccccg ccgggaacac gcgctccatg
     5281 taccagccca gcgcgtccgg caggctcagc agccgcagcg tctcggcggt gggcgcgcag
     5341 tcgaccacca agaggtcgta gttgccggcc tcgacgtggt cgcgcacgtc gaccagcgcg
     5401 aagatctcct ccaggcccgg gatgaccgac agctcctccg cctcgatctc cccgacgccg
     5461 ccccactgca gcaggccgat caggtagccc tggatgtcgc gccagtgctc ctccaggcgc
     5521 gcctgcgcgt cgatctgctc ggcgtccagg cgcggggcga cgctgacggg ccggtcgccc
     5581 agcggcacgt cgagcgcgtc ggccagcgag tgcgccgggt ccgtggacac caccagggtg
     5641 cgcaggccgg cggcggcggc gcgcagcgcg ctcgccgcgg cgacggacgt cttgccgacg
     5701 ccgcccttgc cggtgaacag caggacgcgc acgcggggcc taggccagcg actcgacgcg
     5761 ctgcttcatg cccttcaggg ccgagtccac gatcttcttg gtggccttgc gcttgaggat
     5821 gccgggcacg gagatggtgg ggtcgacctc gagctggtag gtcaccttgg tctggccgcc
     5881 gccggcgtcc tccagcgtgt agctgccgtc gaccttgcgc acctgctcgc cctccaccag
     5941 gtgccacgac atcgacgtgt ccgtgtaggt gtaggccagc gtgtagctgg tggtcatgat
     6001 catggcgtcc agcacgaagc ggaccttggt ggcccagccg tcgtcgtcgg tctccagcac
     6061 ctcgacgctt ttgaagtccg gctgccagtc cgggtaggcg tcgaagtccg cgatcacgtc
     6121 catgatcgtg tcggcgtccg ccttcacgag gatgctctcc cgcaccgtct cgctcacgtc
     6181 ctgctcctct cctgccgtgg agggggaatc ctagggcgtg ggcggggccg ggcgcgcaac
     6241 gctcagggcg cggcgacgcc gcccccctgc tccgcggcct ggcccgcctg ggcggccgcg
     6301 accttgcgcg cgtgggcctc gacccccgcc tggaactcgg cgatggcgtc gcgctggtcg
     6361 gccagcaggc gcgggtcggg gtccgtgccg ggcaggcggc cgccctcgca gcggtccttg
     6421 agggcgtgca gcgcctcgcg cgcgacggcg cggtggtcgc gcgtcaggcg gcgggcgcgg
     6481 cgggcgcctc cgcgggtctc cacgcggagc aggtagttga cgaccacgcc gttgggctcg
     6541 tcggtgtaga agaactctgc ctcgccggtc agcgcgcggc ccgtgaagcg gtaggacacg
     6601 ccgaggttcg ggcggcgctt gacgatctcg acggtgacgc ggtggctgcg gcgcagcagg
     6661 ccgggcggcc ggaacgccag ctcgacgcgg tccgcgcccg cgccgcgggc gcgcacgccc
     6721 ggccaccact ggccgtagcg gtccagaggc agggcgagca gcgcgtgcac gtcgcgccgc
     6781 cgcgcgcgca cgaacccggt gtcccacgtc tcgatccgca tgacgccccg caggctaccc
     6841 gccccatccc cccgagttgg aagatcaggg ccgtatgtgg gccacatctt ccaacttgga
     6901 gggtcaggcc cgtatgtggg ccagatcttc caactcgggc gatcaggtga gcagcagggc
     6961 gagccaggcc agggccgcca gcccgtagcc gctggcagcg cgcagcaccg tcgcgcgctc
     7021 ctgcccgcgc accgcgctcc acaccatgcc cagcaggaag ccggccagga agccgcccag
     7081 gtgggcctgc caggcgatct gcggcaccag gatgggcagc gccgcgttga tcccgagcag
     7141 catgagcatc tgccgcagct gcgcctggcc catcacggtg tggcggttgg ccacggcgat
     7201 cgccgtccag gcgccgaaca ggccgaagat cgcgcccgag gcgccgaccg cgaagccgcc
     7261 cggctcgccc agccacaggt acgccagccc gccgcacaaa agcgacgcca ggtacatgcc
     7321 gccgaacgcg gtggagccgg cctcgcgctc cagcggcggc ccgaacgccg acagcgccca
     7381 catgttgaac aggatgtgcg tgaagccggc gtgcaggaag ccggccgtca ccacccggta
     7441 gctgtcgccc gccagcacgg aggcgttgtg ctggccgaac cacaggaaca gctcgcggcc
     7501 caccgcgggc gccagcaggc ccagcacgaa cacggcggcg gccacgccga tgacgcccat
     7561 gctgaccggg gcggtgtcgc ggcggaccgc gcggatgcgc gggccggtgt cgcgcgcgca
     7621 ctccgggcag cgctggccga ccggcgccgg cgtgacgcag tcccagcaca ccggccggtc
     7681 acagtgcgtg cacgccagcc gcgtctcccg gtcggggtgg cggtagcaga cggggatggt
     7741 tcgctcggct tccacgccgt ccaccctaat gggactgcaa cggctctgct gaatgcggca
     7801 gggtgttgtc cgagccgacc tggcgggtga gcactgcgtt gcccagggcc attgtgagcc
     7861 ctatggctag accgtgcccc cgtcgggcgg tgaggaggag tgacgactgg tgggatggcg
     7921 tgcctgttcg agcactgatc cattaggccg tgcgcgtgcc tcctcctgcg gctcgagacc
     7981 gtgtccttgc ggcttccccg attggagata ggcgagatga tcttcgtcgg cattgactgg
     8041 tcggagcgtc atcacgacgt ggaggtgcaa cgcgatgacg gcaccacggt ggagcgggca
     8101 cgcgtgggtg aggggctgga aggcgccgcc aggctgcatg ccctgctggc cgcccacgcc
     8161 gaggatcccg gcgaggtcgt gatcggcatc gagaccgacc ggggcctgct ggtcggctcg
     8221 ctggcggctg ccggctatca ggtgttcgca gtcaatccga aagcagtcag ccgttaccgg
     8281 gaccgccacg cgatgtcggg ggcaaagtcc gacgcccggg acgccaaggt cctggccgat
     8341 ctggtgcgca ccgaccggca caaccaccgg ccgatcgccg gtgacagcga cctggccgcg
     8401 gcgatcaaag tgaccgcccg ggcgcaccaa aacctgatct ggacccggca acgccagcta
     8461 aatcagctgc gcaacacgtt gcgcgactac tacccggcag cgctggaagc gttcggcacc
     8521 gacctggcgc accctgacgc ggtcgcggtt ctgaccaagg cgcccacacc cgaacgcggc
     8581 cggcgcctgt cgaccgcgca gatcgcctcg gcgctgcgcc gcgcgggccg caaacgcaac
     8641 atcgacagcc gcgccgcggc gatccgcgac gcactacgca caccgcaact gtcggcgcca
     8701 ccagcggtcg ccgacgccta cggcgacacc accaaggcct tgttcggcgt gatcggcgca
     8761 ctgaccgccc agatcagcga gctcgaagcc gcgctgaccg accgttttga gcagcacccg
     8821 gacgccgaca tctaccgcag tctggccgga cttggtgtca tcctcggcgc ccgggtgctc
     8881 gcggaattcg gggacgaccc cgaccgctac gccgatgcta aagcccgccg caactacgcg
     8941 gggagctccc ccatcaccaa agcctcgggc acacgccgcg tcgtgctcgc ccgctacgtc
     9001 cgcaaccgtc gcctcgccga cgcgctcaca tggtgggcgt tctgctccct gaccacctct
     9061 cccggagcca gagcgttcta cgaccgtcac cgcgccaacg gccacaccca ccaccaagca
     9121 cttcgcgccc tgtcgaaccg ctgggtcggc atcctccacg gctgtctgcg ccaccacacc
     9181 ccctacgacg aaaccgccgc ctggagacac cgcaacgagc aactcccaac cgctgcttga
     9241 cgcactccag ccgtgggatg tctagccgtc ctcctccgag gccccgccaa gaccgcgggc
     9301 caggcccacg ccggcggcga ggcggcggtg ctcctcggcg gccaggcgca ggaagccctc
     9361 cgccataccg gcgccgtcca gtcccccggc gccgctgcgg taggccgcca gccccgcgca
     9421 cacgcgcacg tagtccaccg actccagggc ggtcgcgtag cggcgcagcg ccgtctcctt
     9481 gcgcgcccag acggccgtga cgtccagcag cgcgtccacc ggcgcggccg cccacacctc
     9541 gtagccgtac gtgtcggcgt ccaggcccgc ggcggcgacc gccgcgttcg cggcgcggtg
     9601 gtcggcgtgc gggtccagca gggacggcag gtacaccgtg cgcgcccccg cgccgtgccg
     9661 ggccaccgcc tcggccagct ccccggcgtg gcccgccagc gcgccgtcgg gcaggcgcag
     9721 gaacaccggc tcgccgatgc cgaggtccgc gcaggcggcg cggcactccg cctcgcgcgc
     9781 cgcggccacg tcgccgctgc cgccacgggt cgcctcgccg ctggtggcca ccaggaccgt
     9841 cacggcgtcg ccgcggcggg cgtggcgcgc cagcgcgcca ccgcagccga tggtctcgtc
     9901 gtcggggtgg ggcgccacca ccaggaccgg tccctcggga gggccgggca gcacggcggg
     9961 accggcggcg gcgcgcgcgc ccagcgccag ggcgagctcg cgcaggtccg gggacagcga
    10021 tcgggccgcc ccccgggcgg cccgatgcag cagtctgcgg gcgcggctca cttctggtac
    10081 aggccctcga tcgcctcgcc gaagtggtcc accacgttct tgcgccgcac cttcagcgac
    10141 ggcgtgagct cgtcgtcctc gatggtgaag tcgcgttcca ggatggcgaa cttgcggatc
    10201 gcctcggcct tgctgaccgc ggcgttgccg tggtcgatcg ccttctgaac ctcggcgagc
    10261 accgccgggt cgtccttgag ctgggcgacc gtggtgccgg acaggccgtg ctccttggcg
    10321 aaggccgcca gctcctcggg ctccagggtg atcaggcagg ccacgaacgg ccggttgtca
    10381 ccgacgacca tcgcctggga caccaggcgg ttggccttga tgcgctcctc cagcacggcg
    10441 ggcgcgacgt tcttgccgcc ggccgtgacg atgatctcct tcttgcggcc ggtgatggcc
    10501 aggtagccct ggtcgtccag ctcgccgatg tcgccggagt ggaaccagcc gtcgtcctcc
    10561 agcacctccg aggacgcggc gtcgttcttg tagtagccgg tgaagacgtt gccgcccttg
    10621 atgaggatct cgccgtcctc ggcgatcttg atctccacgc cgggcagcgg ctggccgacc
    10681 gtgccgatct tgaacgcctg cggggagttc gccgttgccg gggcgctggt ctccgtgagg
    10741 ccgtagccct ccaggatggt gatgccggcg gcgtggaaga agtgcgcgag gtgcggggcc
    10801 agcggcgcgc cgccggacac gcagtagcgc acgcgcccgc cgagcgcgtc gcgcagcttg
    10861 gagtagacga gcttgtcggc cacggcgtgc ttcacgttga cgcccaggcc cgggttgcgg
    10921 ccttcgttct tggcttcgct ccacgcgtac gcggtctgca ccgcgaagtc gaacaccttg
    10981 cgcttggggc cctcggcctt gcgctgcgcg ccgttgaaca ccttctcgaa cacgcgcggc
    11041 acgctcagca ggaacgtggg cgagaagctg accaggtcct cctggagctg gtcgatgccg
    11101 cgcgagtagc ccagcgtcac gtcgctctcc aggcagccga actggatcag ccgcgcgaac
    11161 acgtgcgcca gcggcaggaa cagcagcgtg gactcctcgc cgccgaacag ctcgcgcagg
    11221 ttctgctcgg tctgccgcgc cgtccacacc aggttgtggt gggtgagcat gcagcccttg
    11281 gggttcccgg tcgtgcccga cgtgtagatg atcgaggcga ggtcggcggc cttgaccttc
    11341 ttggcccgct ccagcacctg cgcgcgcccg gcgtcgtcgc cgcgctcggc cagcgcgtcc
    11401 aggccggcgt cggcgaacac gaagatctcg ctgaggttct tggcctgctc gcgcgcctcg
    11461 tccaggttct tggcgtgctc ggccttctgc gcgaacgcga gcttggcgcc ggagtcggac
    11521 aggatccagg cgcactgctc cacggagctg gtctcgtaga tcggcacggt gacgccgccg
    11581 acgctgagga tggccaggtc ggcgatggtc cagtccaggc acgtgccgct catcagcgcg
    11641 acgcggtcac ccggctcgat cccggcggcg atgagcccgg cgccgacggc ggcgacgcgg
    11701 tcgccgagct gcttccaggt caggccccgc cacgagccgt tcgtccagtg gcgcaggatc
    11761 tcgcgaccgg tggacgtggc ggcgcgctcc cacagccgcg tggtgaggtt ctggtcgggc
    11821 gcgacctcga ctggggtcct gcccttgacg tgcgtgatgg ccatgtcctc gctccctggc
    11881 atgttctggc gttggcggtg cggcagagcc tatgcgaccg cgacagcgcg cattcttacc
    11941 ggatcaggca gccggcacgc cccccggaga ctgctcggga gactgccccg gagaccgctc
    12001 gggtcggcgc tcggcgtcgc ggagaaaggc ccggaccgcc gtgagccacc tctccgggtt
    12061 ctccttctgt ggcgaatggt aactgacggg gatcacggcg cgctcggccc ccgggatggc
    12121 ggcggcgagg gcccgctgct cgggctcctg cca
//
LOCUS       DATMKC010000056         3593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_69257, whole genome shotgun sequence.
ACCESSION   DATMKC010000056 DATMKC010000000
VERSION     DATMKC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69257"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VNU01_01570"
     CDS             complement(<1..333)
                     /locus_tag="VNU01_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HWH31340.1"
                     /translation="MGKVAVMGAGSWGTAYAMLCADAGEDVVLWARRAEVADEVNREH
                     RNSGYLGDVVLPDTLRATPEAEEALEGAEIVVLGVPSVGLRDALSAWKPIVPRDAIAV
                     SLVKGIELD"
     gene            417..1022
                     /gene="cofC"
                     /locus_tag="VNU01_01575"
     CDS             417..1022
                     /gene="cofC"
                     /locus_tag="VNU01_01575"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HWH31341.1"
                     /translation="MDTVAVVPLKALDRAKERLSPHLDAGARRALAGWMLGRVIEACA
                     GCRHVQRVLVVAGDEHGAELAVAAGAEALLQPVPGLTASLDAADAMLAGAAATLVVAA
                     DLPLALAEDLERVVEAAPDGPAVVVAPTSDGGTAALYRRPGRVIGTAYGPGSADAHLS
                     LARAAGAAGVRLHLPRLALDIDTPQQLAEWRSRFPASLPGA"
     gene            1089..1295
                     /locus_tag="VNU01_01580"
     CDS             1089..1295
                     /locus_tag="VNU01_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31342.1"
                     /translation="MAQGTIKEFDITTRSGVVALDNQDELPIDAETFAASGLLELRLG
                     QRVRFSIEGDGDSRRVRDLDLVSF"
     gene            1442..2386
                     /locus_tag="VNU01_01585"
     CDS             1442..2386
                     /locus_tag="VNU01_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH31343.1"
                     /translation="MFALAEEMGSVRGACRAMGVHPSTFYRWKKLVERYGLEALRPRE
                     RRHPRMPNATSVFVEHRVLAFAIAHPGFGPARIAAELTRDKWGGIVLSPNGVWRILKR
                     HGLNTRAKRLALVAGYAAPPELDREADKQPERHINAEKPGDVVQFDCFQIGRLKGTSG
                     VAWQYTAIDVASSYTWAEIWVTPKNPSPRWTSTLARRVAADLAEHGWQLGRVTTDGGG
                     EFRANEFRDTVAKLGADHVVVTRPQSNGCVERVQLTILNECWRPAFARHLIPRYTALR
                     HDLERYLRYYNTDRAHTGRNNKGRVPIEILGKAKMWAH"
     gene            complement(2525..2779)
                     /locus_tag="VNU01_01590"
     CDS             complement(2525..2779)
                     /locus_tag="VNU01_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31344.1"
                     /translation="MATKAAPKKAAKKAPAKKKAAAKKPAAKKAAAKKAPAKKAPAKK
                     APAKKAAAKKAPAKKAAARKAPAKKAPARKVAKKVTPRKK"
     gene            complement(3010..3279)
                     /locus_tag="VNU01_01595"
     CDS             complement(3010..3279)
                     /locus_tag="VNU01_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31345.1"
                     /translation="MDQAGRIGGDFPGEEPGQRPGQVDAVAFMRVSDGLRAAFAELGA
                     TKLRADEAARWHRRLIAISNVAKRDLPGALEQLERFEHDWSHRRK"
     gene            3357..>3593
                     /locus_tag="VNU01_01600"
     CDS             3357..>3593
                     /locus_tag="VNU01_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31346.1"
                     /translation="MTTSPATESSPPLRASTALAVIGAAGAALMAIAHMGVDVPLLPT
                     QGLGRAVPVAAAAFSVGAVVFAATAWGLARRARWA"
BASE COUNT          462 a         1365 c         1229 g          537 t
ORIGIN      
        1 gtccagctcg atgcccttga ccaggctcac cgcgatggcg tcgcgcggca cgatcggctt
       61 ccacgccgac agcgcgtcgc gcaggccgac ggacggcacg ccgagcacca cgatctcagc
      121 gccctccagc gcctcctccg cctccggggt ggcgcgcagc gtgtcgggca ggaccacgtc
      181 gcccaggtag ccggagttgc ggtgctcccg gttgacctcg tcggccacct ccgcccggcg
      241 cgcccacagc acgacgtcct cgccggcgtc ggcgcagagc atcgcgtacg cggtgcccca
      301 cgacccggcg cccatgaccg cgaccttgcc catcggggtc ctttctggcc gactgcttgc
      361 cacgtgctcg gcagggcgcc gcgagtctac gcgccggccg cgctaccgtg agcggcatgg
      421 acaccgtcgc cgtcgtgccg ctgaaggcac tggaccgggc caaggagcgc ctgtccccac
      481 acctggacgc cggtgcgcgc cgggcgctgg ccggctggat gctcggccgc gtcatcgagg
      541 cgtgcgcggg ctgccggcac gtccagcgcg tgctggtggt ggccggcgac gagcacggcg
      601 ccgagctggc cgtggccgcg ggcgccgagg cgctgctgca gccggtcccc ggcctcaccg
      661 cgtcactgga cgccgccgac gccatgctcg ccggcgccgc ggccaccctg gtggtggccg
      721 ccgacctgcc gctggcgctg gccgaggacc tggagcgggt ggtggaggcg gcaccggacg
      781 ggcccgcggt ggtggtggcg cccaccagcg atggcggcac ggccgcgctg taccggcggc
      841 ccgggcgcgt catcggcacc gcctacgggc cggggtcggc cgacgcccac ctgtcgctgg
      901 cccgcgcggc cggcgcggcc ggcgtgcgcc tgcacctgcc gcgactggcc ctggacatcg
      961 acacgcccca gcagctggcg gaatggcgca gccgtttccc ggcgagcctc cccggcgcgt
     1021 aacctgcagc gggccggcgc gagcgggccg gtcagcgtga cgcgggacgt ggaaggagcc
     1081 gggacctcat ggcgcagggc accatcaagg aatttgacat cacgacccgg tcgggcgtcg
     1141 tggccctgga caaccaggac gagctgccga tcgacgcgga gaccttcgcc gcatccgggc
     1201 tgctcgagct ccggcttggc cagcgtgtgc gcttctccat cgagggcgac ggcgacagcc
     1261 ggagggtccg cgacctcgac ctggtgagct tctaggctgt cctatccccg agtgatggcg
     1321 acacgtcggg ggttcggctc ctgacacagc acagcggcgc atcctgctgc ttgaccgcca
     1381 agtcgagcaa aggagcgccg ctgtgagcgt tgatgatgcc ttgttccgct tccgtgtgcg
     1441 ggtgttcgcc ctggccgagg aaatgggctc ggtgcggggc gcgtgccggg cgatgggcgt
     1501 gcatccctcg acgttttacc ggtggaagaa gctggtcgaa cgttacgggc tggaggcgct
     1561 gcgaccgcgg gagcgtcgcc accctcggat gcccaacgcg acgtcggtgt tcgtcgagca
     1621 ccgggtcctc gcgttcgcga tcgctcaccc ggggttcggg ccagcgcgca tcgccgcgga
     1681 gctcacccgt gacaagtggg gcgggatcgt gctgtcaccc aacggggtgt ggcgcatcct
     1741 gaagcgccat gggctgaaca cacgcgccaa gcgcttggcg ttggtcgccg gctacgccgc
     1801 cccacccgag ctcgaccgtg aggccgacaa gcagcccgag cggcacatca acgcggaaaa
     1861 gcccggcgat gtcgtgcagt tcgactgctt ccagatcggc cggctcaagg gcaccagcgg
     1921 cgtggcgtgg cagtacaccg ccatcgatgt cgcctcctcc tacacgtggg ccgagatctg
     1981 ggtcaccccc aagaacccct cgccccggtg gaccagcacg ctcgcccgcc gtgtcgccgc
     2041 cgatctcgcc gagcacggct ggcaactcgg ccgcgtaacc accgacggcg gcggggagtt
     2101 ccgcgcgaac gagttccgcg acaccgtcgc caagctcggc gcagaccacg tcgtggtgac
     2161 ccgcccgcag tccaacggct gtgtcgaacg ggtccagctg acgatcctca acgagtgctg
     2221 gcgacccgcc ttcgcccgcc acctcatccc tcgctacacg gccctgcgac acgacctcga
     2281 acgctacctg cgctactaca acaccgaccg cgcccacacc ggccgcaaca acaagggccg
     2341 agtgcccatc gagatcctcg gcaaggccaa gatgtgggca cactgaccac cacccgcagc
     2401 aggaagcgtc gctgctactc ggggacagga cattctaggc ggcccgccgg aagcccgcga
     2461 acggcagagc gcccgggaga tcccgggcgc tctggatttt ccgacctctt gtgccgcgga
     2521 gcggctactt cttgcgggga gtgaccttct tcgccacctt ccgcgcgggg gccttcttgg
     2581 ccggcgcctt cctggccgcc gccttcttgg ccggcgcctt cttggccgcc gccttcttcg
     2641 cgggcgcctt cttggccggc gccttcttcg cgggcgcctt cttggccgcc gccttcttcg
     2701 cggcaggctt cttcgccgcc gccttcttct tcgcgggggc cttcttggcc gccttcttgg
     2761 gtgctgcctt ggtcgccacg ggaccgcctt cctcgccggg cagtgccgac gtctctcaac
     2821 cttggccgac tgaaggcggg ctctcgccgc ctgtgctccc cgcgcactcc accgccggaa
     2881 ttcccgcagt gtgagcagca cgcgagatcc ctgcggacgt taggcgttcg cgcacacgta
     2941 atcaacgatc cgcgacgaag cgtgtctggg catcgcgccg ctcgccgcga cccgcttcgt
     3001 cgctcaagct cacttgcggc ggtgggacca gtcgtgctcg aagcgctcca gctgctccag
     3061 cgcgccgggc aggtcgcgct tggcgacgtt cgagatcgcg atcaggcgcc ggtgccagcg
     3121 ggcggcctcg tccgcgcgca gcttcgtcgc ccccagctcc gcgaacgcgg cgcgcagacc
     3181 gtcggacacc cgcatgaacg ccacggcgtc cacctgaccc ggacgctgtc cgggctcttc
     3241 tccggggaag tcgcccccga tccggcctgc ctgatccatc tgctcccctg ctgtgcggcc
     3301 gctccaccgt agcgccccct gcccgggccc cgcggggatc gcattagcat ggcccggtga
     3361 ccacctcccc cgccaccgaa tcgtcgccgc cgctgcgcgc ctccaccgcg ctggccgtga
     3421 tcggcgccgc gggcgccgcg ctcatggcca ttgcgcacat gggcgtggac gtcccgctgc
     3481 tgccgacgca aggcctcggc cgcgccgtcc ccgtcgccgc ggcggccttc tccgtgggcg
     3541 ccgtggtttt cgcggccacg gcatggggcc tggccaggag agcgcgctgg gcc
//
LOCUS       DATMKC010000057         3633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_189287, whole genome shotgun sequence.
ACCESSION   DATMKC010000057 DATMKC010000000
VERSION     DATMKC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189287"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="VNU01_01605"
     CDS             complement(<1..1903)
                     /locus_tag="VNU01_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWH31347.1"
                     /translation="MPQSLEQEAVDVAIGISEDHEALAAAIRGWAERADVRGAAREAL
                     EGPETRPQWWSGLADQGLLGLHVPEESGGSGAGGVELAVTVEELARSLASGPALPTLL
                     ASLLVQRAGGTAAKELLPALVDGSATAAVALAPGGLTATPSADGVTVRGSVRPVVGAG
                     LADLLVLGAEADGGPVWFVVEAAQAQVESLQGLDATRRPAQVVLDDVVVPDARVLRGL
                     DAAAVRTLSAAVVAAEAAGLAGWALETAVDYAKTREQFGRPIGVFQGIKHKCADMLVR
                     AEQARATAWDAARALDEPLAPEAELAVTVAAAVAVQAALDNAKDALQVLGGIGYTWEH
                     DAHVVLKRALSLRALIGDPARWRGRAADLALGGARRHLALDLGEDTAPVREQVRAFLG
                     TLDGLDDAARKAAIADSGYLVPHWPQPWGRNANAVEQLVIDEEFAAAGVRRHDLIIGG
                     WAAPTIVAHGTPEQQERFVRPTLHGEIVWCQMFSEPGAGSDLAALQTRAEKVEGGWKV
                     NGQKVWTSMAQIADWAILLARTSGTGKGPDRHRGITYFLLDMKTPGVDVRPLRELTGH
                     AAFNEVFLDDVFVPDDCVVGEVGGGWPLARTTLANERVAMSSGSPFGRGVEDVLSDVA
                     ARGGFGEDPV"
     gene            2040..2648
                     /locus_tag="VNU01_01610"
     CDS             2040..2648
                     /locus_tag="VNU01_01610"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWH31348.1"
                     /translation="MAMASTTTTSPDVETAAQRDRRSRIVQAALALASRGGYDDVQMR
                     EVAERSGVALGTLYRYFPSKVHLLVNAMAHELERMSSRLQRRTIPGDTPSARVEWVLE
                     RATRSLQKDPNLTEAMIRAMMAADSTVAVEVAAVRSQMGGMIIAAIRGDREAHPDDAE
                     YAAVLQDVWFARQIAWLGGRISSADVWRDIESAIRLVLRDHE"
     gene            complement(2666..>3633)
                     /locus_tag="VNU01_01615"
     CDS             complement(2666..>3633)
                     /locus_tag="VNU01_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH31349.1"
                     /translation="ASIEACDKTFAGVGSVSAFDQGGAPVIQQCGMPDIRGLSTTNPM
                     KKVPNAFPINAAGTGGARSTGMFGWAAEKFPDAIKKAAYVYIDGEVTRELANEDMEAT
                     KAVLGYNWIYSKAIGITETNYGPVVQQLKNSGAEYVTFTGAQEQAASLAKQFKTQGFE
                     PTVWQPTVTAYTPNYISGAGAAAEGTYIAVTASLNEEMGINPELKTYAQWLNQVAPGK
                     TPTGIGQFAWGAAALFVEKMKEIGPKPTRKALLATLPKVRSYTGNGLFPGQDVGGRQL
                     SSCTKVVQVKGGKFVRFTPEKPGSNRCVDKIYDTKTKKASEGFPD"
BASE COUNT          480 a         1352 c         1280 g          521 t
ORIGIN      
        1 gcaccgggtc ctcgccgaag ccgccgcgcg cggcgacgtc ggacagcacg tcctccacgc
       61 cgcgaccgaa cggcgagccc gagctcatcg ccacccgctc gttcgccaag gtcgtacgag
      121 ccagcggcca gccgccgccg acctcgccga cgacgcagtc gtcggggacg aacacgtcgt
      181 cgaggaagac ctcgttgaag gcggcgtgcc cggtgagctc ccgcagcggc cgcacgtcga
      241 cgccgggggt cttcatgtcg agcaggaagt aggtgatccc ccggtgccgg tccggcccct
      301 tgccggtgcc gctggtgcgg gccagcagga tcgcccagtc ggcgatctgc gccatcgacg
      361 tccagacctt ctggccgttg accttccagc cgccctcgac cttctcggcg cgcgtctgga
      421 gcgcggccag gtccgagccc gcgcccggct cgctgaacat ctggcaccag acgatctcgc
      481 cgtgcagggt gggacgcacg aagcgctcct gctgctccgg cgtcccgtgc gccacgatcg
      541 tcggcgccgc ccagccgccg atgatgaggt cgtgccgccg cacccccgcg gcggcgaact
      601 cctcgtcgat gaccagctgc tcgacggcgt tggcgttgcg gccccagggc tgcggccagt
      661 gcggcacgag gtagccggag tcggcgatcg ccgccttgcg cgcggcgtcg tccaggccgt
      721 ccagggtgcc gaggaaggcg cgcacctgct cgcgcaccgg cgcggtgtcc tcgccgaggt
      781 cgagcgccag gtgccggcgt gcgccgccca gcgcgaggtc ggcggcgcgc ccccgccacc
      841 gggccgggtc gccgatcagc gcccgcagcg agagcgcgcg cttgaggacg acgtgggcgt
      901 cgtgctccca cgtgtagccg atgccgccga gcacctgcag cgcgtccttc gcgttgtcca
      961 gcgcggcctg cacggccacc gccgcggcga ccgtcaccgc gagctcggcc tccggtgcca
     1021 gcggctcgtc gagcgcgcgt gcggcgtccc acgcggtggc ccgcgcctgc tccgcgcgca
     1081 cgagcatgtc ggcgcacttg tgcttgatcc cctggaagac gccgatcggc cgcccgaact
     1141 gctcgcgcgt cttcgcgtag tcgaccgccg tctcgagcgc ccatccggcg agaccggccg
     1201 cctcggccgc gacgacagcg gccgacagcg tgcgtacggc agcggcgtcc agcccgcgca
     1261 gcacgcgcgc atccggcacc acgacgtcgt cgaggacgac ctgggccggg cgccgcgtcg
     1321 cgtcgagccc ctgcagcgac tccacctgcg cctgcgctgc ctcgacgacg aaccagaccg
     1381 ggccgccgtc tgcctcggca cccagcacca gcaggtcggc cagcccggca cccacgaccg
     1441 gacggacgct gccgcgcacc gtgaccccgt cggcgctcgg agtggcggtg aggccgccgg
     1501 gtgccagcgc cacggcggcg gtggccgagc cgtcgacgag ggccgggagc agctccttgg
     1561 cggccgtgcc gcccgcgcgc tggaccagca ggctggccag cagcgtcggg agcgccggcc
     1621 cggaggccag cgaacgcgcc agctcctcga cggtgacggc cagctccacg ccgcccgcgc
     1681 cggagccgcc ggactcctcc ggcacgtgca ggccgagcag cccctggtcg gccagcccgg
     1741 accaccactg cggacgcgtc tccggcccct cgagcgcctc gcgggcggcg ccgcgcacgt
     1801 ccgcacgctc ggcccacccg cggatcgcgg cggcgagcgc ctcatggtcc tcgctgatcc
     1861 cgatcgccac gtcgaccgcc tcctgctcga gagattgcgg catactagaa caggttcttg
     1921 ttctcgcgca ggtatccgct gtgcacgtga tgcatgagac accgagaact ggctagaacc
     1981 ggtgctactt tcgctccttc acggggcgct gctcttgtcc cagggagagg cgggaacgca
     2041 tggcgatggc cagcaccacg acgacgtcgc cggacgtgga gacagcggca cagcgcgacc
     2101 gccgctcccg catcgtccag gcggcgctgg cgctcgccag ccgcggcggc tacgacgacg
     2161 tgcagatgcg cgaggtcgcc gagcgcagcg gcgtcgcgct gggcacgctg taccgctact
     2221 tcccctccaa ggtgcacctg ctcgtgaacg cgatggcgca cgagctcgag cgcatgagca
     2281 gccggctgca gcgccgcacc atcccgggcg acacccccag cgcccgcgtc gagtgggtgc
     2341 tggagcgggc gacccggtcg ctgcagaagg accccaacct caccgaggcg atgatccggg
     2401 cgatgatggc cgccgactcc accgtcgcgg tggaggtcgc cgccgtccgc agccagatgg
     2461 gcggcatgat catcgcggcg atccgcggcg accgtgaggc gcacccggac gacgcggagt
     2521 acgccgccgt cctgcaggac gtgtggttcg cgcggcagat cgcctggctc ggcgggcgca
     2581 tcagcagcgc cgacgtgtgg cgcgacatcg agtcggcgat ccggctcgtc ctgcgcgacc
     2641 acgagtagcc gcgcaggcgt ctctgctagt cggggaagcc ttccgacgcc ttcttggtct
     2701 tggtgtcgta gatcttgtcg acgcagcggt tgctgcccgg cttctccggc gtgaagcgca
     2761 cgaacttgcc gcccttcacc tgcacgacct tggtgcagct ggacagctgg cgcccgccga
     2821 cgtcctgccc ggggaacagg ccgttcccgg tgtagctgcg caccttcggc agggtcgcca
     2881 gcagcgcctt gcgggtcggc ttcgggccga tctccttcat cttctcgacg aacagcgcgg
     2941 cggcacccca ggcgaactgc ccgatcccgg tcggcgtctt gcccggcgcc acctggttga
     3001 gccactgcgc gtacgtcttc agctcggggt tgatgcccat ctcctcgttg agcgaggcgg
     3061 tgacggcgat gtaggtgccc tcggccgcag cgcccgcgcc ggagatgtag ttcggggtgt
     3121 acgcggtgac ggtcggctgc cacacggtcg gctcgaagcc ctgggtcttg aactgcttgg
     3181 ccagcgaggc ggcctgctcc tgcgcaccgg tgaaggtgac gtactcggcg ccggagttct
     3241 tcagctgctg cacgaccgga ccgtagttgg tctcggtgat gccgatggcc ttggagtaga
     3301 tccagttgta gccgaggacc gccttggtgg cctccatgtc ctcgttcgcg agctcgcggg
     3361 tgacctcgcc gtcgatgtag acgtaggccg ccttcttgat ggcgtcgggg aacttctcgg
     3421 cggcccagcc gaacatgccg gtgctgcggg caccgccggt gccggcagcg ttgatcggga
     3481 aggcgttcgg caccttcttc atcgggttgg tcgtcgacag gccgcggatg tccggcatgc
     3541 cgcactgctg gatgaccggc gcgccgccct ggtcgaacgc cgacaccgag ccgacgccgg
     3601 cgaaggtctt gtcgcaggcc tcgatgctgg cgc
//
LOCUS       DATMKC010000058        14369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_15801, whole genome shotgun sequence.
ACCESSION   DATMKC010000058 DATMKC010000000
VERSION     DATMKC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15801"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..730
                     /locus_tag="VNU01_01620"
     CDS             134..730
                     /locus_tag="VNU01_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWH31350.1"
                     /translation="MRDVVVLAFEGVQSLDVTGPLEVFASASAALPDGRGYRVAVLTP
                     DGAPVRSSSGLGLLPHGTLADAPARIDTLVVAGGDGTAAAMADPRLLGWLSGAAARCR
                     RVTSVCSGAFLLAEAGLLDGRRATTHWSVCGALARRYPAVTVEPDPIFVRDGDVWTSA
                     GVTAGMDLALALVEDDHGRDIALAVARRLVLFLRRPAN"
     gene            788..1102
                     /locus_tag="VNU01_01625"
     CDS             788..1102
                     /locus_tag="VNU01_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH31351.1"
                     /translation="MQAWVAEHLDADLTVEALARRAAMSSRHFARAFRQETGATPARY
                     VERLRVEAARRRLEESGDAVERIAAECGFGTAETMRRAFLRVVGVGPAEYRRRFRAPA
                     HH"
     gene            1114..1761
                     /locus_tag="VNU01_01630"
     CDS             1114..1761
                     /locus_tag="VNU01_01630"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWH31352.1"
                     /translation="MRIAIPLYDRMTALDAIGPYEVLARIPGAEVVFVGHAAGEVRAD
                     QRLGLTADAVLEDVPAPEVVLVPGGPGQADLMGDERLLSWLRRTHETTTWTTSVCTGS
                     LLLAAAGVLEGRRATSHWLALEALRRFGAEPVQERWVEDGKIITAAGVSAGIDMALEL
                     ASRLAGPQVAQGIQLGIEHDPQPPFDAGSPSKAPDDVVAFLRANSRFVLEGRPTT"
     gene            1824..2906
                     /locus_tag="VNU01_01635"
     CDS             1824..2906
                     /locus_tag="VNU01_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31353.1"
                     /translation="MPPFKESVNEGSVRALARRLSAAGAFPARRFTADATRGLESLEL
                     KARIAHVVGALERHLPGDFAEAARLVEDAVAGSDLGMWSAWPATDWVAAAGLDHPEIA
                     LPLLARLTGVASAEMAIRPFIDRHPARTLRQLRTWTSHPDEHVRRLVSEGSRPRLPWA
                     SRIALLEAEPHLTVPLLDALREDSSPYVRRSVANHLNDLNKVDRGLALETASRWSAQG
                     GQHVEAVLRHGLRTLVKAGDAEALGLVGAPPARVRLADLEVLTPRVRLGGSLRFRFTV
                     TSQEREPMRAVIDYLVHHRKANGGTTQKVFKLSTRTLAPGAPVTIERAHAIRPITTRR
                     YYPGEHRVEVQVNGAVLGGGTFILDP"
     gene            complement(2929..3567)
                     /locus_tag="VNU01_01640"
     CDS             complement(2929..3567)
                     /locus_tag="VNU01_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HWH31354.1"
                     /translation="MSAPSQDPAPARLRVDYPEEGLDRTSTLLRLLYAIPVAVLLGLI
                     GHMNGPSFGGEDAVQIGTFGGGVLVLPVLLTIVFRQKYPRWWFDFNLQLARFSARVLA
                     YLSLMTDRYPSVDEEQSVHLDLAYPDAREDLNRWMPLVKWLLAVPHYVVLLFLGIASV
                     VATVIAWFAILFTGRYPRGLFDFVEGVLRWKLRVEAYALLLITDRYPPFSLR"
     gene            3826..4164
                     /locus_tag="VNU01_01645"
     CDS             3826..4164
                     /locus_tag="VNU01_01645"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWH31355.1"
                     /translation="MARAATTSDVFNAIAEPQRREILALLRAGERPVTELARELGMTQ
                     PGASKHLRVLREVGLVRDRRAGKQRLYALDARGLRPVHEWTGGFAQFWNASFDRLDEY
                     VQDLKHTRQE"
     gene            4168..4674
                     /locus_tag="VNU01_01650"
     CDS             4168..4674
                     /locus_tag="VNU01_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH31356.1"
                     /translation="MAATAGDTPAQSPTADREIVISRVIGAPRELVFEAFTDVRHLSR
                     WWGPEGFTTTTRAFEFRVGGVWDFVMHGPDGTDYQEWITWTEIAPPERIALLHGESRG
                     DPDAFESVLTFQLDGAATRIEMRTVFPTRELRDEAVEKYHAIEGGTQTLDNLAAYVTE
                     VLRSGEQR"
     gene            4674..5294
                     /locus_tag="VNU01_01655"
     CDS             4674..5294
                     /locus_tag="VNU01_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWH31357.1"
                     /translation="MAGKVFFSVTMSLDGFIAPESIEDLMKRQWMELHQWMFPQRFFR
                     ENLKLGEGGEEGRDNDIARETFERTGASVMGKRMFDAGERAWPEEAPFRTPVFVVTHE
                     QRDPWVRPGGTTFHFVNDGIASALDQAREAAGDRDVRIAGGGATILEYVNAGLIDEFS
                     IALSPVLFGSGIRLFEGVDAGRVALEPAGTESSSRVTHLTYTVRQR"
     gene            complement(5412..5487)
                     /locus_tag="VNU01_01660"
     tRNA            complement(5412..5487)
                     /locus_tag="VNU01_01660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5452..5454),aa:Arg,seq:ccg)
     gene            5685..6053
                     /locus_tag="VNU01_01665"
     CDS             5685..6053
                     /locus_tag="VNU01_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH31358.1"
                     /translation="MMACDDDHVIRGLLEVNLEMEGYDVVTASDGQDALDKVRDNPPD
                     LILLDVMMPNLNGWQVAEALQSDESTRGIPIIFLSARAMEADVQKGTSLGAASYVTKP
                     FDPIDLMELVAEHLRKRGIT"
     gene            6122..6976
                     /locus_tag="VNU01_01670"
     CDS             6122..6976
                     /locus_tag="VNU01_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HWH31359.1"
                     /translation="MGIAERGIAAAPGVDRPGDHSCLVYDDAEQRAEHTAFWVRHGLD
                     RGERVVYVQPPGMAGQFQDELASWGLDVTAPLREGRVVLLSPEQALLVDGDWDVARRM
                     ALHEAFVRESVGQGFAAVRMGADAAAAMAHVPGLAALSRYEKGMESLTLRLPVSVLCL
                     YERRAFGQAFGGVALAHPRGMGDRQMHIVAKPGHVTLVGEVDMSNTQLVASALREAAT
                     LDGRLVVDLAGVSFIDLGGTTQLVDLARRLGGRDCVRVVGAPRPLRRILGAAGWTDEL
                     DLVGGGVG"
     gene            6973..7101
                     /locus_tag="VNU01_01675"
     CDS             6973..7101
                     /locus_tag="VNU01_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31360.1"
                     /translation="MTAGGTAPPSGFQHEELTSEGRLPRPPADHRTLRLARGPRDL"
     gene            7242..8846
                     /gene="argS"
                     /locus_tag="VNU01_01680"
     CDS             7242..8846
                     /gene="argS"
                     /locus_tag="VNU01_01680"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWH31361.1"
                     /translation="MIGPDQLAPLVRQALSAAGLPDREPAFERPRQREHGDWATNVAL
                     TLAKPVGKPPREVAESIVAHLPAHDGVDAVEVAGPGFLNFRLSHGAFQDIVREAVRRA
                     AGWGRAEPAQPLGSVNVEFVSANPTGPLHVGAGRWAAAGDAIAALLDAAGWTVTREYY
                     LNDAGNQMELFAESVHAVAQGRPVPENGYQGAYVSELAAELAAAGVDAADLGEQAYRR
                     TRARIEATLERFGVTYDVWFSERSLHDSGAVDRAVARLREGGWVDEVDGALFLRTTEL
                     GDDKDRVLIRSDGRPTYFAADCAYMLSKAERGATRAVYLLGADHHGYVRRLQAVARAL
                     GLGEDWLEVIIGQLVNLYRGGEAVRMSKRTGEMVTFDELIDEVGRDAARYTFLRTSLD
                     VTLDFDIAEVVKQERDNPVYYVQYSHARIAGILRTARERGLEPGAVDDARLELLTHPA
                     EEELIRRVGAYPETVAYAAAERAPHKVARYAEDLAEGFHRFYTECQVVGEDAELSRAR
                     WWLVMAAKQTLVNALGLLGVDSPDRM"
     gene            8852..10171
                     /gene="lysA"
                     /locus_tag="VNU01_01685"
     CDS             8852..10171
                     /gene="lysA"
                     /locus_tag="VNU01_01685"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWH31362.1"
                     /translation="MDESLRRVFPRTAVFEHGRLMAVGGVDVEALAAEHGTPLWVLDR
                     HELVSRMRAYREAFGDDVGVIYAAKALSVTGVLQLAAREGLMVDVASGGELRSAEVAG
                     VPMASVVFHGNNKSVEELEHAARTGVGRVVVDSFTELERLADVAARTGHTFRVQLRVT
                     PGVDTDTHAHIRTGHDDSKFGFTLSTGLADEAVDRAVALPGVELVGLHSHVGSQILAG
                     DAFESAVTAMVELLVRMRLRHGVELPELNLGGGLGIAYLRDDLPVPIEEYARRLLAAV
                     DAECASLGVPRPRLFVEPGRSIAGPAGLTLYRVGTVKDIPGVNTYVAIDGGMSDNPRP
                     ALYGARYEFVPAGPGKPVEQTRAVAIAGKHCETGDVLARDVALPVDLAEGELLAVAAT
                     GAYNQAMASNYNRLPRPAMVLAGDGRADLLLRRETLDDLLAHDVPLP"
     gene            10266..11552
                     /locus_tag="VNU01_01690"
     CDS             10266..11552
                     /locus_tag="VNU01_01690"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWH31363.1"
                     /translation="MSERTLTVGLLGAGVVGSGVVRLIDEHAGEISARLGARLALGPV
                     AVRDLSRARDVAPARLTTDPGEVVNDPEVDIVVEVMGGIEPARALITAALENGKSVVT
                     ANKALISTHGIELIELARAKGVRLEFEAAVAGAIPIIKPMRESLAGDRVRKVLGIVNG
                     TTNFILTRMTEEGMDFADALAEAQRLGFAEADPTADVEGFDAAAKAAILATLAFDAKV
                     VADDVYREGITGVTAQDIAFADRMGYVIKLLAIAEADADGVGVRVHPALVPRTHPLAS
                     VRESFNAVFVEAEAAGDLMFYGRGAGSLPTASAVMGDIVTAARALLSGERQELGAQLT
                     DKPLRPFSDTVVQYYVLLDVADEPGVLASVAAAFGQHRVSIKSVWQEGQGSGAQLLLI
                     THAAREADVRATLEDLRGLDAVRDVASVMRVEGSEV"
     gene            11555..12625
                     /gene="thrC"
                     /locus_tag="VNU01_01695"
     CDS             11555..12625
                     /gene="thrC"
                     /locus_tag="VNU01_01695"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWH31364.1"
                     /translation="MAAAAGVHQWRGLIEEYRDRLPVETSTPVITLREGGTPLIHSEV
                     LSERTGCDVWLKFDGANPTGSFKDRGMTMAISKAVERGAKAVVCASTGNTSASAAAYA
                     AKAGLVCGVLIPKGKIALGKLAQALVHGAQVIQIEGNFDQALAICRTLDEKYPIELVN
                     SVNPFRIEGQKTGAFEVCDFLGRAPDVHVMPVGNAGNITAYWKGYREYRDDGVTDGLP
                     VMRGFQAAGAAPIVNGAIVENPSTIATAIRIGNPASWTQAVAAADESGGSIRAVTDRD
                     IMKAYRSLALEGVFCEMASASSVAGLLQLHGAGDLAPGQTVVCVLTGHGLKEPDWAIT
                     GAATPAVIPPDVDRAAELLGLA"
     gene            12634..13521
                     /gene="thrB"
                     /locus_tag="VNU01_01700"
     CDS             12634..13521
                     /gene="thrB"
                     /locus_tag="VNU01_01700"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWH31365.1"
                     /translation="MTAHDGIGVSVPATSANLGPGFDAFGVALTMSLRARTAPREERR
                     VVPSGDGAAELPTGDDNLVWRAFTTYCEHFGAAVPDVTLLVDNDIPLERGMGSSSAAA
                     VAGIVLARAVTGAGGTHQDLVDLAAGFDGHPDNVAPAVLGGLVVCVAGKATRLQPSGR
                     LRPVVCVPETRQNTHEARGVLPASVPLADAAANGARAALVLAGLAGAAAWDPAAMADV
                     LHEPVRLEVMAASGALVRALRAAGIGACLSGAGPSVLAVVPRADAVGLERVRAEAGDG
                     WRVLPLDWDLAGTRLLRRA"
     gene            13853..>14369
                     /locus_tag="VNU01_01705"
     CDS             13853..>14369
                     /locus_tag="VNU01_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HWH31366.1"
                     /translation="MDRSVLARKQLTELKDIAASLEMRGYQRLKKAELVEAIVAKSGG
                     GADRNGNGAGRAARDADSTASTPEPRAERPSDGDDEGRVRVRTRTRDRGERDDAADGG
                     QTQDRAADGPDTSSDKSPDKSPEQSGGEPAQARESNAGGSGQAGQGGGQERDRGGDGQ
                     RQRQGGQGGGDR"
BASE COUNT         1991 a         5116 c         5339 g         1923 t
ORIGIN      
        1 tcccccgagt tggaagatca gggtcgtatt tggcacagat cctccaattc acaggctgcg
       61 gcgagacctg gatggcagaa actgagggat atccggcatt gccgccagtc ggcacaagcc
      121 gcatgctgag ccgatgcgcg acgtggtggt acttgccttc gaaggcgtcc agagcctgga
      181 tgtcaccggg ccgctcgagg tcttcgcctc ggcgagcgcc gcgctgccgg acggccgcgg
      241 ctaccgcgtc gccgtcctga cgccggacgg cgccccggtg cgcagctcgt cgggcttggg
      301 gctgctgccg cacggcacgc tggccgacgc cccggcgcgc atcgacacgc tcgtggtcgc
      361 cggcggggac ggcaccgcgg ccgccatggc cgacccccgg ctgctcggct ggctgtccgg
      421 cgccgcggcg cgctgccgcc gggtgacgtc ggtgtgcagc ggcgcgttcc tgctggccga
      481 ggccggcctg ctggacggcc gccgcgccac cacccactgg tcggtctgcg gcgcgctcgc
      541 gcgccggtat cccgccgtca ccgtggagcc ggatcccatc ttcgtgcggg acggcgacgt
      601 gtggacctcg gccggggtga ccgccggcat ggacctggcg ctggcgctgg tggaggacga
      661 ccacggccgc gacatcgcgc tggcggtggc gcgccggctg gtgctgttcc tgcgccggcc
      721 ggcgaactag gcgcagttct ccgcgcacct ggcggcgcag ctcgccgacc gagcgccgct
      781 gcgggaattg caggcgtggg tcgccgagca cctcgacgcc gacctgaccg tcgaggcgct
      841 ggcgcgccgc gccgcgatga gctcccgtca cttcgcgcgc gcgttccggc aggagaccgg
      901 cgccacgccc gcccgctacg tcgagcggct gcgcgtcgag gcggcgcgcc ggcggctgga
      961 ggagtccggc gacgcggtgg agcgcatcgc cgccgagtgc gggttcggaa cggccgagac
     1021 gatgcgccgg gcgttcctgc gcgtcgtcgg tgtgggcccc gcggagtacc ggcggcgctt
     1081 ccgcgcgccg gcgcatcatt gacaggagaa gccgtgcgca tcgcgatccc gctgtatgac
     1141 cggatgaccg cgctcgacgc catcgggccg tacgaggtgc tggcccgcat ccccggcgcc
     1201 gaggtggtgt tcgtcggcca cgccgccggt gaggtgcgcg ccgaccagcg gctcggcctg
     1261 accgccgacg ccgtgttgga ggacgtgccg gcgcccgagg tggtgctggt cccgggcggt
     1321 ccgggccagg ccgacctcat gggggacgag cgcctgctgt cgtggctgcg ccgcacccac
     1381 gagacgacga cgtggacgac gtcggtctgt accggctctc tgttgctcgc ggccgcgggg
     1441 gtcctggagg gccggcgcgc gacgtcgcac tggctcgcgc tcgaggcgct gcgccgtttc
     1501 ggcgcggagc cggtccagga gcgctgggtc gaggacggca agatcatcac ggccgcgggc
     1561 gtgtccgcgg gcatcgacat ggcgctggag ttggcgtcgc gcctcgcggg gccgcaggtg
     1621 gctcagggca tccagctcgg gatcgagcac gacccgcagc cgccattcga cgccggctcg
     1681 ccgtccaagg cgccggacga cgtggtcgcc ttcctgcggg cgaactcgcg cttcgtcttg
     1741 gagggccgcc cgacgaccta gccgatcggg gcggccgcgg ccgctttggc ggggctgaca
     1801 cagccgcacg gcacactggc ggcatgccgc cgttcaagga gtcggtcaac gaggggagcg
     1861 tgcgcgctct cgcccggcgc ctgtcggcgg cgggcgcatt cccggcccgg cgcttcacgg
     1921 ccgacgccac gcgcggcctt gagtcgctgg agctcaaggc gcggatcgcg cacgtggtgg
     1981 gggcgctgga acgtcacctg ccgggggact tcgccgaagc ggcgcggttg gtggaggacg
     2041 ccgtcgccgg atccgacctg ggcatgtggt cggcgtggcc ggcgaccgac tgggtggcgg
     2101 ccgcagggct ggaccatccc gagatcgcgc tgccgctgct ggcgcggctg accggggtcg
     2161 ccagcgcgga gatggcgatc cgccccttca tcgaccgcca cccggcacgg acgctgcgcc
     2221 agttgcgcac gtggacctcg caccccgacg agcacgtgcg gcgcctggtc agcgaaggca
     2281 gccgaccgcg gctgccctgg gcgtcgcgca tcgcgttgct ggaggccgag ccgcacctga
     2341 cggtgccgct gctcgacgcc ttgcgagagg attccagccc ctatgtgcgg cggtcggtcg
     2401 ccaaccacct gaacgatctc aacaaggtcg accgggggct ggccctcgag acggcgtcac
     2461 gctggtctgc ccagggaggg cagcacgtcg aggcggtgct gcgccacggt ctgcgcacgc
     2521 tggtgaaggc cggtgacgcc gaggcgctgg ggctggtcgg cgcgccgccc gctcgcgtgc
     2581 ggctggcgga cctggaggtg ctcacccccc gcgtgcgcct cggcgggtcg ctgcgtttcc
     2641 gtttcacggt cacgtcgcag gagcgcgagc cgatgcgggc ggtcatcgac tatctcgtgc
     2701 accatcgcaa ggccaacggc ggcaccaccc agaaggtgtt caagctgtcg acgcggacgc
     2761 tggcgcccgg cgccccggtg acgatcgagc gggcgcacgc gatccgtccg atcaccaccc
     2821 gccgctacta cccgggggag caccgcgtcg aggtccaggt caacggcgcg gtgctcgggg
     2881 gcggaacgtt catcttggac ccgtgagtcc cgcgcggcgc gccactcctc agcgcagcga
     2941 gaacggggga tagcggtccg tgatgagcag cagggcgtag gcctccaccc gcagcttcca
     3001 gcgcagcacc ccttcgacga agtcgaacag cccacgcggg tagcggccgg tgaacaggat
     3061 cgcgaaccac gcgatgacgg tcgccaccac cgacgcgatg cccaggaaga gcagcacgac
     3121 gtagtgcggc acggccagca gccacttgac caggggcatc cagcggttga ggtcctcccg
     3181 cgcgtcggga tacgcgaggt cgaggtggac cgactgctcc tcgtccaccg agggatagcg
     3241 gtcggtcatg agcgacaggt aggcgagcac cctggcggag aagcgcgcga gctggaggtt
     3301 gaagtcgaac caccagcgcg ggtacttctg ccggaagacg atggtcagca gcactggcag
     3361 gaccagcacg ccgccgccga aggtgccgat ctgcacggcg tcctcgccgc cgaaggacgg
     3421 gccgttcatg tgcccgatca ggcccagcag gaccgcgacc ggaatcgcgt agaggaggcg
     3481 tagcagcgtc gacgtgcggt cgagaccctc ttcgggatag tcgactcgca gccgggcggg
     3541 ggcggggtct tgcgatgggg cactcatggc acgttcctct tcgttgtcgg aaccgttcgc
     3601 gacaggctgc gccgcgccat gcgcaatgcc gcacggcgct caggtgtgct gctccggccg
     3661 acctcccggc gccgggagcc ccccggagca ccttacgtcc gaattggggg ctcgaactca
     3721 tcctggacgg attggagcgg gcgcgcgccg gccgtcgagc cccgaccacg gtccaggccg
     3781 gcaggttccg gtttacacat accgatatag gaatatgatg atcccatggc acgagcagcc
     3841 acgacgtcgg acgtcttcaa cgcgatcgcc gagccgcagc gccgggagat cctcgcgctg
     3901 ctgcgggcgg gtgagcggcc ggtgaccgag ttggcccggg agctcggcat gacgcagccg
     3961 ggggcgtcca agcatcttcg ggtgctccgg gaggtcggtc tggtgcggga ccgcagggcg
     4021 ggcaagcagc gcctgtacgc cctcgacgcg cgggggctgc gaccggtcca cgagtggacc
     4081 ggcgggttcg cgcagttctg gaacgcaagc ttcgaccggc tcgacgagta cgtgcaggac
     4141 ctcaagcaca caaggcagga gtgaccgatg gcagcgacag caggggacac gccggcgcag
     4201 tcgccgacgg cggaccgcga gatcgtgatc tcgcgggtca tcggcgcccc gcgggagctg
     4261 gtgttcgagg cgttcaccga cgtgcggcac ctgtcgcgct ggtggggtcc ggagggcttc
     4321 accaccacca cgcgggcgtt cgagttccgc gtcggcgggg tgtgggactt cgtgatgcac
     4381 ggacccgacg ggacggacta ccaggagtgg atcacctgga ccgagatcgc cccgccggaa
     4441 cggatcgcgc tgctccacgg tgagtcccgc ggcgacccgg acgccttcga gtccgtcctc
     4501 acgttccagc tcgacggcgc ggcgacccgg atcgagatgc gcacggtgtt tcccaccagg
     4561 gagctgcgcg acgaggcggt cgagaagtac cacgccatcg agggcggcac gcagaccctg
     4621 gacaacttgg ccgcctacgt caccgaggtg ctgcgcagcg gagagcagcg ctgatggccg
     4681 ggaaggtgtt cttcagcgtc acgatgtcgc tggacggctt catcgcgccc gagtccatcg
     4741 aggacctgat gaagcggcag tggatggaac tgcaccaatg gatgttcccg cagcggttct
     4801 tccgggagaa cctgaagctc ggcgagggcg gcgaggaagg gcgcgacaac gacatcgcgc
     4861 gggagacgtt cgagcgcacc ggcgcgagcg tgatgggcaa gcgcatgttc gacgccggcg
     4921 agcgggcgtg gccggaggaa gcgccgttcc gcacgcccgt gttcgtcgtg acgcacgagc
     4981 agcgagaccc gtgggtgcgg ccgggcggga cgaccttcca cttcgtcaac gacggcatcg
     5041 cgtccgcgct cgaccaggcc cgcgaggcgg ccggcgaccg ggacgtccgc atcgcgggcg
     5101 gcggcgcgac gatcctggag tacgtgaacg cgggtctgat cgacgagttc tcgatcgcgc
     5161 tgtcacccgt cctgttcggc tccggcatcc gcctgttcga gggcgtggat gcgggccgcg
     5221 tggccctgga gccggctggt acggagtcct cctcaagggt gacgcacctg acgtacaccg
     5281 tccggcagcg gtaaccgagc cggccagtac agcatcggcg accgatcgga gcgcgaagtg
     5341 gtccggcacg ccctctggtc caacgcacaa tgtcccgaga caggtgtgaa ccatgtctcg
     5401 ggacatcaca ttggtgcccg cggcaggatt cgaacctgcg gccttctgct ccggaggcag
     5461 acgctctatc ccctgagcta cgcgggcggg ggctcctcga agaggccggc ctactgtagc
     5521 accgggtctc gggcgcccca ccggccgtga agggccagcc ataccccggt ctgccaccta
     5581 tttggtcgcc cgaatggggg gtacatcgca tctacggcca cgccgaccgg actagcatgg
     5641 ccgcatctac cctcatgttg cgacaggagc agggcttggc acgggtgatg gcctgtgatg
     5701 acgaccacgt catccgcggg ttgctcgaag tgaacctcga gatggagggc tatgacgtcg
     5761 tcaccgcctc cgatggccag gacgccctgg acaaggtgcg cgacaacccc ccggacctga
     5821 tcctgttgga cgtgatgatg cccaacctca acggctggca ggtggccgag gcgctgcagt
     5881 cggacgagtc caccaggggc atccccatca tcttcctgtc ggcgcgcgcc atggaggccg
     5941 acgtgcagaa gggcaccagc ctgggcgccg cgtcctacgt caccaagccg ttcgacccca
     6001 tcgatctgat ggagttggtc gccgagcacc tgcgcaagcg cggcatcacc tgaccgccgc
     6061 gcgacgagtc aggacaaacc cgtcatctgt ccctcctggt catcacacct aggctgggcc
     6121 gatgggcatc gccgaacggg gaatcgccgc ggcgccgggc gtcgaccggc ccggcgacca
     6181 cagctgcctc gtctacgacg acgccgagca gcgcgccgag cacaccgcct tctgggtgcg
     6241 ccatggcctg gaccgcggcg agcgcgtcgt ctacgtgcag ccgcccggca tggccgggca
     6301 gttccaggac gagctcgcgt cgtggggcct ggatgtcacc gcgccgctgc gtgagggccg
     6361 cgtcgtcctg ctgtctccgg agcaggccct cctggtcgac ggcgactggg acgtcgcccg
     6421 gcgcatggcg ctgcacgagg cgttcgtgcg cgagtccgtc gggcagggat tcgcggcggt
     6481 gcgcatgggc gcggacgcgg cggccgcgat ggcgcacgtc cccgggctgg cggcgctctc
     6541 gcgctatgag aagggcatgg agagcctgac cctgcggctg cccgtgtccg tgttgtgcct
     6601 gtacgaacgc cgtgcgttcg gtcaagcttt cggtggcgtc gcactcgcgc acccgcgggg
     6661 gatgggggac aggcagatgc acatcgtcgc caagccgggc cacgtgacgc tggtcggcga
     6721 agtcgacatg tccaacactc agctcgtggc cagcgcgctg cgcgaggccg cgaccctgga
     6781 cggccgcctg gtcgtcgacc tggcgggcgt gtcgttcatc gacttgggcg gcaccacgca
     6841 gctggtcgac ctggcacgcc gcctcggcgg ccgcgactgt gtccgtgtgg tcggcgcgcc
     6901 gcggccgctg cggcgcatcc tcggcgcggc cggctggacc gacgagctcg acctggtcgg
     6961 cggtggcgtg ggatgaccgc cggcggcacc gcgccgccat ccggcttcca gcacgaggag
     7021 ctcacctccg agggcaggct gccccggccg ccggccgacc accggacgct gcgcctcgcg
     7081 cgcggcccgc gggacctgtg aacgagctcg cgaccgacag cgtgcgccac ggcggcggtg
     7141 agggcgtcct gcggctgtcc cgagccgggc gcggtcatct gcgaggctga gccggccggg
     7201 ccgcttcgcg ggcgcccgcg cgccgcgtct accattcggc catgatcggc cccgaccagc
     7261 tcgccccgct cgtccgccag gcgctttccg ccgccggact gcccgaccgc gagcccgcgt
     7321 tcgagcggcc gcgccagcgc gagcacggcg actgggccac caacgtggcg ctcacgctgg
     7381 ccaagccggt gggcaagccg ccgcgcgagg tcgccgagtc gatcgtggcg cacctgcccg
     7441 cccacgatgg cgtggacgcc gtggaggtcg cgggaccggg cttcctcaac ttccgcctgt
     7501 cgcacggcgc gttccaggac atcgtgcgcg aggccgtgcg gcgcgccgcc ggctggggcc
     7561 gcgcggagcc ggcgcagccc ctgggcagcg tcaacgtcga gttcgtcagt gcgaacccca
     7621 ccgggccgct gcacgtgggc gccgggcgct gggccgcggc cggcgacgcg atcgccgcgc
     7681 tgctggacgc cgccggctgg accgtcaccc gcgagtacta cctcaacgac gccggcaacc
     7741 agatggagtt gttcgccgag agcgtccatg cggtcgcgca gggccggccg gtgcccgaga
     7801 acggctacca gggcgcctac gtctccgagc tcgcggccga gctggcggcc gccggcgtgg
     7861 acgcggccga cctcggcgag caggcctacc gccgcacccg cgcgcgcatc gaggcgacgc
     7921 tggagcgctt cggcgtgacc tacgacgtgt ggttcagcga gcgctcgctg cacgactcgg
     7981 gtgccgtgga ccgcgccgtg gcgcgcctgc gcgagggcgg ctgggtggac gaggtcgacg
     8041 gcgcgctgtt cctgcgcacc accgagctcg gcgacgacaa ggaccgcgtg ctcatccgct
     8101 ccgacggccg cccgacgtac ttcgccgccg actgcgccta catgctgagc aaggccgagc
     8161 gcggcgccac gcgcgccgtc tacctgctcg gggcggacca ccacggctac gtcaggcgcc
     8221 tccaggcggt ggcgcgcgcc ctggggctgg gcgaggactg gctcgaggtg atcatcggcc
     8281 agctcgtgaa cctgtaccgg ggcggcgagg cggtccgcat gagcaagcgg accggggaga
     8341 tggtcacctt cgacgagctg atcgacgagg tcggccgcga cgcggcccgc tacacgttcc
     8401 tacgcacctc gctggacgtc acgctggact tcgacatcgc cgaggtggtc aagcaggagc
     8461 gcgacaaccc cgtctactac gtgcagtaca gccacgcgcg catcgccggg atcctgcgca
     8521 ccgcgcggga gcgcgggctt gaacccggtg ccgtggacga cgcgcgcctg gagctgctca
     8581 cgcaccccgc cgaggaggag ctgatccgcc gcgtgggcgc ctacccggag acggtggcct
     8641 acgccgcggc cgagcgcgcg ccgcacaagg tcgcccgcta cgccgaggac ctggccgagg
     8701 gcttccaccg cttctacacg gagtgccagg tcgtcggcga ggacgcggag ctgtcgcgcg
     8761 cgcgctggtg gctggtgatg gccgccaagc agacgctggt gaacgcgctg ggcctgctgg
     8821 gcgtcgactc gccggatcgg atgtgagcgc catggacgag tccctgcggc gcgtcttccc
     8881 gcgcacggcc gtgttcgagc acgggcggct gatggcggtc ggcggcgtgg atgtggaggc
     8941 gctggccgcc gagcacggca ccccgctgtg ggtgctggac cgccacgagc tggtctcgcg
     9001 catgcgcgcc taccgcgagg cgttcggcga cgacgtcggc gtgatctacg ccgccaaggc
     9061 gctcagcgtg accggcgtgc tccagctcgc ggcgcgcgag gggctcatgg tggatgtcgc
     9121 cagcggcggc gagctgcgca gcgccgaggt cgcgggcgtg ccgatggcct cggtggtctt
     9181 ccacggcaac aacaagagcg tcgaggagct ggagcacgcg gcgcggaccg gcgtgggacg
     9241 cgtggtggtg gacagcttca ccgagctgga gcgcctggcc gacgtcgcgg cgcgcaccgg
     9301 ccacaccttc cgcgtgcagc tgcgcgtgac gcccggggtg gacaccgaca cacacgcgca
     9361 catccgcacc ggccacgacg actccaagtt cggcttcacg ctgtccaccg gcctggccga
     9421 cgaggcggtg gaccgcgcgg tggccctgcc cggcgtcgaa ttggtcggcc tgcacagcca
     9481 cgtgggcagc cagatcctgg ccggcgacgc gttcgagtcg gcggtcacgg cgatggtcga
     9541 gctgctggtg cgcatgcgcc tgcgccacgg cgtcgagctg cccgagctca acctgggcgg
     9601 cgggctgggc atcgcctacc tgcgcgacga cctgcccgtg cccatcgagg agtacgcgcg
     9661 gcggctgctc gccgccgtcg acgccgagtg cgcgtctctg ggcgtgccca ggccgcggct
     9721 gttcgtggag ccgggacggt ccatcgccgg tccggccggg ctgacgctgt accgggtcgg
     9781 cacggtgaag gacatccccg gcgtgaacac ctacgtggcc atcgacggcg gcatgagcga
     9841 caacccgcgc ccggcgctgt acggcgcgcg ctacgagttc gtgccggccg gccccggcaa
     9901 gccggtggag cagacccgcg cggtcgccat cgcgggcaag cactgcgaga cgggcgacgt
     9961 gctcgcgcgc gacgtcgcgc tgccggtgga cctggccgag ggggagctgc tcgcggtggc
    10021 cgcgaccggc gcctacaacc aggccatggc cagcaactac aaccggctgc cgcgccccgc
    10081 catggtgctg gccggagacg ggcgcgccga cctgctgctg cgccgcgaga cgctggacga
    10141 cctgctcgcc catgacgtgc cgctgccctg accccgcggg gttgggtgcg gtttcagagc
    10201 catggctctg aaaccgcacc cacacaggcg cggaagctgt ggacgcgacc cgtagactcc
    10261 cccgcatgag cgaacgcacc ctcaccgtcg gcctgctcgg cgcgggcgtc gtcggctccg
    10321 gcgtggtgcg cctgatcgac gagcacgcgg gcgagatctc cgcgcgcctg ggagcccgcc
    10381 tcgcgctggg gcccgtcgcc gtgcgcgacc tgtcccgcgc gcgcgacgtg gcgcccgcgc
    10441 gcctgaccac cgaccccggc gaggtcgtca acgaccccga ggtcgacatc gtcgtggagg
    10501 tcatgggcgg catcgaaccg gcccgggccc tgatcacggc ggcgctggag aacggcaaga
    10561 gcgtcgtcac ggccaacaag gcgctgatct ccacgcacgg catcgagctc atcgagctgg
    10621 cgcgcgccaa gggcgtgcgg ctggagttcg aggccgccgt ggccggcgcc atccccatca
    10681 tcaagccgat gcgcgagtcg ctggccgggg accgcgtccg caaggtgctg ggcatcgtga
    10741 acggcaccac caacttcatc ctcacgcgca tgaccgagga gggcatggac ttcgccgacg
    10801 cgctggccga ggcccagcgc ctcggcttcg cggaggcaga ccccaccgcc gacgtcgagg
    10861 gcttcgacgc ggcggccaag gcggcgatcc tggccacgct cgcgttcgac gcgaaggtgg
    10921 tcgccgacga cgtctaccgc gagggcatca ccggcgtcac ggcgcaggac atcgccttcg
    10981 ccgaccgcat gggctacgtc atcaagctgc tggccatcgc cgaggccgac gccgacggcg
    11041 tcggcgtgcg cgtgcacccg gcgctggtgc ccaggacgca cccgctggcc agcgtgcgcg
    11101 agagcttcaa cgccgtgttc gtggaggccg aggccgccgg cgacctcatg ttctacggcc
    11161 gcggcgccgg ctcgctgccc accgccagcg ccgtcatggg cgatatcgtc acggccgcgc
    11221 gcgcgctgct gtccggtgag cgccaggagc tgggcgccca gctcaccgac aagccgctgc
    11281 ggccgttctc cgacaccgtg gtgcagtact acgtgctgct ggacgtggcc gacgagcccg
    11341 gcgtgctcgc cagcgtcgcg gccgccttcg gccagcaccg cgtgtccatc aagagcgtgt
    11401 ggcaggaggg ccaggggtcc ggagcccagc tgctgctgat cacgcacgcc gcccgcgagg
    11461 ccgacgtgcg cgccaccctg gaggacctgc gcggcctgga cgcggtgcgg gatgtcgcca
    11521 gcgtcatgcg ggtcgagggg agcgaggtct agccatggcg gcagcggcgg gcgtgcacca
    11581 gtggcggggg ctgatcgagg agtaccgcga ccgcctgccg gtcgagacgt ccacaccggt
    11641 gatcaccctg cgcgagggcg gcacgccgct gatccactcg gaggtgctgt cggagcgcac
    11701 cggctgcgac gtgtggctga agttcgacgg cgccaacccc acgggctcgt tcaaggaccg
    11761 cgggatgacc atggccatct ccaaggcggt ggagcgcggc gccaaggccg tggtgtgcgc
    11821 ctccaccggc aacacctcgg cgtcggccgc cgcctacgcg gccaaggccg gcctggtctg
    11881 cggcgtgctg atccccaagg gcaagatcgc gctgggcaag ctggcgcagg cgctggtgca
    11941 cggcgcgcag gtcatccaga tcgaaggcaa cttcgaccag gcgctggcga tctgccgcac
    12001 gctggacgag aagtacccca tcgagctggt gaactccgtc aatcccttcc gcatcgaggg
    12061 ccagaagacc ggcgcgttcg aggtgtgcga cttcctgggc cgcgcgcccg acgtgcacgt
    12121 catgcccgtg ggcaacgccg gcaacatcac cgcctactgg aagggctatc gcgagtaccg
    12181 cgacgacggc gtgaccgacg ggctcccggt gatgcgcggg ttccaggccg ccggcgcggc
    12241 gcccatcgtc aacggcgcga tcgtcgagaa cccctcgacc atcgccaccg cgatccgcat
    12301 cggcaacccc gcgtcgtgga cccaggcggt cgcggcggcc gacgaatccg gcggctccat
    12361 ccgcgccgtc accgaccgcg acatcatgaa ggcctaccgc tcgctggcgc tggagggcgt
    12421 gttctgcgag atggcgtcgg cctccagcgt cgccgggctc ctgcagcttc acggggccgg
    12481 cgacctcgcc cccggtcaga cggtggtgtg cgtgctgacc ggccacgggc tcaaggagcc
    12541 cgactgggcg atcaccggcg cggccacgcc ggccgtcatc ccgcccgacg tcgaccgggc
    12601 cgccgagctg ctgggactgg catgagccct ggcgtgaccg cgcacgacgg gatcggcgtc
    12661 tcggtgcccg ccacgagcgc caacctggga ccgggcttcg acgccttcgg cgtcgcgctg
    12721 accatgagcc tgcgcgcgcg gaccgcgccg cgcgaggagc gccgggtcgt gccctccggt
    12781 gacggcgccg cggagctgcc gaccggcgac gacaacctcg tgtggcgcgc cttcaccacc
    12841 tactgcgagc acttcggcgc cgcggtgccc gacgtcacgc tgctggtcga caacgacatc
    12901 ccgctggagc gcggcatggg ctcctcctcg gccgccgcgg tcgccggcat cgtgctggcg
    12961 cgcgccgtga ccggcgccgg cgggacgcac caggacctcg tggatctcgc ggccgggttc
    13021 gacggccatc ccgacaacgt cgcccccgcc gtgctcgggg gactggtggt gtgcgtggcc
    13081 ggaaaggcca ccaggctgca gcccagcggc cgcctgcgtc cggtcgtgtg cgtgccggag
    13141 acccggcaga acacccacga ggcgcgcggc gtgctgcccg cgtccgtgcc gctcgcggac
    13201 gccgccgcca acggcgcgcg cgccgccctg gtcctggcgg gactggcggg cgccgccgcc
    13261 tgggatcccg ccgcgatggc cgacgtcctg cacgagcccg tgcgcctgga ggtcatggcc
    13321 gcctcgggcg cgctcgtgcg cgcgctgcgc gccgccggta tcggcgcgtg cctgtccggt
    13381 gcgggcccca gcgtgctggc cgtggtcccg cgcgcggacg ccgtggggct ggagcgggtg
    13441 cgggccgagg ccggagacgg ctggcgcgtc ctgcccctgg actgggacct cgccggcacg
    13501 cggctgctcc ggcgggcctg agccgcgcga gcggccctgc cgcggtggtt gccggaacag
    13561 ggtgcggtcg ctacacttcg cccatccccg aattcctggc cgttgacggc ttggtgtgtg
    13621 aggggagatt cgaacaggga cgctcgtccc gcagtcccgt cccgcagcgg tgcggcgtgc
    13681 aaggcatctt cgaggggcct accgattcct atccgtggcg cggcagccgg tcttgacgac
    13741 cgctcgcgat gggctctgtg acgggctacc ggttctctct gcgggttggg ttcccgcaat
    13801 gccaccgcgg ccacgcgaag cgtgggccgt accggacagg agtcgaatga gcatggaccg
    13861 cagcgtcctc gcccggaagc agctcactga gctgaaggac atcgccgcct ccctggagat
    13921 gcgcggctac cagcggctca agaaggccga gctcgtcgag gcgatcgtcg ccaagtcggg
    13981 cggaggcgcc gaccgcaacg gcaacggcgc cgggcgcgcg gcgcgtgacg ccgactccac
    14041 ggcttccacg cccgagcccc gcgccgagcg tccctccgac ggcgacgacg agggccgcgt
    14101 gcgggtgcgc acccgcaccc gcgaccgtgg cgagcgcgac gacgccgccg acggcggcca
    14161 gacgcaggac cgcgcggccg acggcccgga cacgtcttcc gacaagtccc cggacaagtc
    14221 ccccgagcag tccggcggcg agcccgccca ggcgcgcgag agcaacgccg gtgggtccgg
    14281 ccaggccggc cagggcggcg gccaggagcg tgaccgcggc ggggacggcc agcggcagcg
    14341 ccaaggcggc cagggcgggg gcgaccgcc
//
LOCUS       DATMKC010000059        10814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_42712, whole genome shotgun sequence.
ACCESSION   DATMKC010000059 DATMKC010000000
VERSION     DATMKC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42712"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1610..2563)
                     /locus_tag="VNU01_01710"
     CDS             complement(1610..2563)
                     /locus_tag="VNU01_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31367.1"
                     /translation="MIPPPRSDPDSLDLLADWVELACLLAHSVAVFDVAEEFRVGGVA
                     GIDSDQFLDEPRLDGGALLAEGDEAEDLARQVWAVLNDRRAELRSAYPFKVSEGLVTS
                     EGWRAWPAYAFLLLLDVGRRYGLSLEPGHERRFEKIVEASMTGLLRSRAVRFGTPAEP
                     DWPVPLPDRVRYLATLLDLEANEQRLARHHLREKDIALDVVGLLKLAGQDGGVAVLAQ
                     CAVGENWTGKSGEPSLTEWRGLLDWNSVLLKAIAVPWRLPEGVWPHRRVAERFEALVL
                     DRPRLVLGHPDRLLTDAFRDELRAACDSPVAQLRRLQGVAS"
     gene            complement(2560..3639)
                     /locus_tag="VNU01_01715"
     CDS             complement(2560..3639)
                     /locus_tag="VNU01_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31368.1"
                     /translation="MEGFEPRTVDPLQTELDPRNPRLTDEEEGSSQDDLRRILVDKFK
                     VFEVAESIAKSGWLPLDPMVGVEEEGVLRIVEGNRRLAALQLLLDPELAPKNKPQWAG
                     LAGGLTAEVREAISSVEVLVAPSRDHADVESYIAFRHVAGVLPWPALEKASYIAHLVT
                     QGYTYEDIAARISSYPSHVRRHHVAHELVRQAKTLEIDGALQMSTSFGVLLRALQAGG
                     VREFLGMSEPATPQDASDPVPGDHIEQLGEFVRWTFGTAEAPRVLRDSRALTMWAKIL
                     SSPESLRYLRSASEPTFQRAWRKAGGERESVSDALWEASFRLEETLPVLKLYRDDEEV
                     RQAVERCELFLSQAREVLGVPNSKA"
     gene            complement(4002..4340)
                     /locus_tag="VNU01_01720"
     CDS             complement(4002..4340)
                     /locus_tag="VNU01_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HWH31369.1"
                     /translation="MRLEAKKYLYDMQQGASLLAEFVAGRTFADYQADAMLRAAVERQ
                     FEIIGEALSKLARLDADLAAQVSEHRRIIAFRDILIHGYADVDHRLVWDIVETKLPLL
                     RSEIESLLAD"
     gene            complement(4330..4638)
                     /locus_tag="VNU01_01725"
     CDS             complement(4330..4638)
                     /locus_tag="VNU01_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWH31370.1"
                     /translation="MNGDLASHQAEIDELCRRFGVLSLELFGSAARGEDQPGVSDLDF
                     LVEFQPEPPGGYADAYFGLSEALQELFGRPVDLVVSSAIRNPFFRESVERTKVLLYAA
                     "
     gene            5106..6332
                     /locus_tag="VNU01_01730"
     CDS             5106..6332
                     /locus_tag="VNU01_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HWH31371.1"
                     /translation="MVPYLPRLVDTLLAELLAELPAVLLVGPRAAGKTTTAARHAASL
                     VRLDRPAEAAAFRADPDAALASFDEPVALDEWQEVPTVLGAVKRAVDADPRPGRFLLT
                     GSVRADIEAETWPGTGRVVRVGMLPMTVGERLERTTRPLVDRVMDGDALAPATPATDL
                     PGYIALAMQSGFPETLGLSEGSRRRWIESYVDQLLTRDAMLVHKGRDPARLRRYLEAY
                     ALNSAGLVEDKTIYDAAGIDRRTALAYEALLVSLQVVEALPAWTSNRLKRLTLSPKRY
                     LVDPALLAGIIGATESAVMRDGDLLGRVLDTFVAAHLRAEADVARHRARLYHLRQEQG
                     RHEIDLLAEIGAERVIGIEVKATSAPDSSSAAHLTWLRERLGDRFIAGVVLHTGPASF
                     SLGDRITAAPISTLWA"
     gene            complement(6576..6728)
                     /locus_tag="VNU01_01735"
     CDS             complement(6576..6728)
                     /locus_tag="VNU01_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31372.1"
                     /translation="MFIELNGGDWAPHPPDVDEAEAAMLAVAGHEVDEAWFASWLRER
                     VHFSQQ"
     gene            6773..7132
                     /locus_tag="VNU01_01740"
     CDS             6773..7132
                     /locus_tag="VNU01_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH31373.1"
                     /translation="MVPRQPARQHRGLVMQGRVREVDPRGLGQGRSGYTGDLLGPDRN
                     RPRPSLPPRHDLRRVDVPVLVSRGDQTPRYFPPVAEAIVEAMPSAEGRVLNGLGHAPH
                     LTHPEKFVTTVADFLTR"
     gene            7279..8091
                     /locus_tag="VNU01_01745"
     CDS             7279..8091
                     /locus_tag="VNU01_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31374.1"
                     /translation="MTGESEAFKARQRQVWSVGDYGAMAEQYQLSADRLVQRLEIGAG
                     MRVLDIATGTGNAAIAAAITGAEVVGIDLTPELFDEAHWRADELGLAIAWQEGDAEAL
                     PYPDGWFDRAVSAFGIMFAPDPHAAARELVRVLRPGGRFGMANWSLRGPVLAVFEAVA
                     GGQVALPASAAHPMPWGEPQAVRTFFDDLGVELEFAHDHVSWVFADADDGVRWLEEVS
                     GAVIAAKAVLEPRGEWAPVRARLVDAVTQLTRPDIEGVWVQAEYLVTLGRKA"
     gene            complement(8092..8889)
                     /locus_tag="VNU01_01750"
     CDS             complement(8092..8889)
                     /locus_tag="VNU01_01750"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWH31375.1"
                     /translation="MTSDWAGERITVERDGHVLLIGVNRPEKRNAFDLQTIAELSQAY
                     EVLGDDPDCWVGVLFAHGDHFSAGLDLAEVSQDVARVGPRAIAGRGKYDPFGVWRDPV
                     PKPVVMAVNGIAFTLSIELALAADIVVCADDVRFNQLEIGRGILPFGGATIRATRQLG
                     WGNAMRFLLTAEEFGAQEAYRIGLVQEVVPAGRHVERAVEIARRIAAQAPLGVQETLA
                     SARIAARVTEEERRHLEEVLPVVMASQDAAEGLRSFVERREARFTGR"
     gene            complement(8937..9248)
                     /locus_tag="VNU01_01755"
     CDS             complement(8937..9248)
                     /locus_tag="VNU01_01755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH31376.1"
                     /translation="MSEERDMGEGKRLRVVRNETASRFEAYLGDELAARLTYVHTDRG
                     LDLQHTVVEPRYRGQGVGEEFAEAVFDELRLTGERIIPTCPFLSAYIEEHPEHRALVV
                     A"
     gene            9325..10077
                     /locus_tag="VNU01_01760"
     CDS             9325..10077
                     /locus_tag="VNU01_01760"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HWH31377.1"
                     /translation="MDLNSDLGESFGAWRLGEDESLLEVVTSANVACGFHAGDPATMR
                     RTVAAAAARGVVIGAQVSYPDLVGFGRRELGATPEQIHDDVLYQLGALDGICRAAGTR
                     VRFLRPHGALYHRCTRDVAAAEAAVAAARAFDAALGVVAPPGSALLAAAAAAGLATVR
                     EGFADRRYLADGTLAPRGTDGAVLTDPAEVAAQAVAIATGEPVRAAAGAPVEVTADTI
                     CLHGDTPGAAALARAVRAALEAHGVPIAPALA"
     gene            10074..10706
                     /gene="pxpB"
                     /locus_tag="VNU01_01765"
     CDS             10074..10706
                     /gene="pxpB"
                     /locus_tag="VNU01_01765"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HWH31378.1"
                     /translation="MTPHPYGSHAALLLFDDAAEVQPWVRAARDANLPGVVDVVPGAR
                     SVVIVGDPARTDADALIAALRTLSQPGTFVRDTGPTFQVGKAEVEVPVTYDGEDLAEV
                     AARTGLTVDEVVAAHAEAAYEVAFCGFAPGFAYLTGLPAALRLPRRDTPRPRVPAGSV
                     AIAAAYSAVYPQASPGGWWLLGRTALTMWDADRDPPALLQPGDRVRFVAT"
BASE COUNT         1777 a         3829 c         3563 g         1645 t
ORIGIN      
        1 cctcgagatc ttccacaacc gccagcgccg ccactccgcg cttggcatgc tcacacccat
       61 cgaatacgag aaccgacgcc tacacgtcgt ggcatgagaa ttccaccccc accgactccg
      121 gagaaccggg ggaagcccaa cccgtccggg atagcggggg aactccaacc cgtccgggat
      181 agcgggggaa ctccaatcca actcgcccgc accgactcgc aagccgccat cgccgaaggc
      241 acccgcgccc tcatgctcca ccgaggacca ctcctgcttg accagcccta cgccgaatgg
      301 acacacgccg aacgccgtca tattgcagaa cgccaccgac tgctcctcga agccctcgct
      361 gacgcaagcg aacaactagg ggaccccttc ggggcgcggc tctaccgcac acactcctga
      421 cgggagccga acgcgcgtgg cgacgcccgt ggagcgtcgg tcgacctgag tcatttgtta
      481 ggcagccggg cgggcgacag ggagtgcggc cgtcacttcg gcgaaagtgg cccttgggtc
      541 ggcggcagct tgagcaacct ctggctcggc ggtcaacccg gcgagggcaa ctcgaaccaa
      601 ccaagctact cgcgccgcca gtggtggcgg aaccgcatta ccgatgtgtc ggcgcgcggt
      661 gtccagtctg ggactcagcg ggtacgagtc cggaaaccct tgcaggcgag ccatttccct
      721 ggtgcgtaag tgtcggccag tccaatggaa tgggccgttg taggtgacgc gttgtgcggg
      781 gatggtaggc acaggccgct cggggtgagc cttgagaagg aacgaccagt aacgtccccg
      841 gtagcggaac aagccctcag ggtgcccgcg ttcgcgcgtc aacatgaggt agttctcacc
      901 aggtgagact tggcgcagag cctccgcgtg cttcccgcgg ggctgctcgt catgctcagg
      961 ctccagcgct ccctcaagat cagcgatggc ccacccggca gacctcttag gcaagggacg
     1021 cagcaggtcc caatcgactg gtggaccgcc tcgaacccca acgacgaata gacgtcggcg
     1081 ggcttgggcc acaccatgct cgctggcgtc caagatcgta tgagagacgg cgtaccctgc
     1141 ctgctcggcg tcaccgatga atgcatcaag cgctggacga tgcgtcttga agcggagacc
     1201 cggtacgttc tccatgatga aggccgcagg ttgaatgcgg tctacggcct cgccatatgc
     1261 cttgaccagt gagcgggcag gatcgctgcc gttacgtttg tagtccaacc agaatccggc
     1321 gtgggaccac ggtgtacacg ggggtccccc aatgagaagc gccggagcct gggcttcagc
     1381 tgcagtgatg atgcgggaaa gctcgcccgc ctcatataga tccgcaacgt cgatgggcgt
     1441 tgcctgggct tgctgcagta gcgaaaggcc ttctgaatcc aagtcacagg cgaagcgcac
     1501 gtcaaagcct gccgcccgcg cgccaaggtc gaggccccca accccgctaa agaggctgac
     1561 gcaagtaagt tcagtcatgg cctgagctca ccagaccggg ccagccaagt caagaagcca
     1621 ctccctgcaa gcgcctgagc tgggcgactg gactgtcaca ggccgcgcga agctcatccc
     1681 tgaaggcatc cgtcaggagt cgatcagggt ggccgaggac caatcgaggt cggtcgagca
     1741 ccagcgcctc gaacctctct gctacgcggc gatggggcca cactccctca ggtagccgcc
     1801 aaggcaccgc tatcgccttg agcaacacag aattccagtc gaggagcccc ctccactcgg
     1861 tcagtgacgg ctcgcccgac tttcccgtcc agttttcgcc tacggcgcat tgggcgagca
     1921 cggcgacgcc tccgtcctga cctgccagct tcaagaggcc aacaacgtca agagcgatgt
     1981 ccttctcccg caagtgatgg cgggcaagtc gttgctcgtt agcctcaaga tccagcaaag
     2041 tggcgaggta acgcacccgg tccggcaatg ggacaggcca gtcgggttca gccggtgtcc
     2101 caaagcggac cgcccggctg cgaaggagcc ccgtcatcga cgcttcaaca atcttctcga
     2161 agcgtcgctc gtgtccgggc tctaaggaca aaccgtaacg gcggccgaca tccagcagca
     2221 agaggaaggc atacgctggc cacgcgcgcc agccctctga cgtcacgagg ccttcgctca
     2281 ccttgaaggg gtaagcactg cgcaactcgg ccctgcggtc attgaggacg gcccaaactt
     2341 ggcgagcgag gtcctcggct tcgtcgccct ccgcgagcag cgctccgcca tccagtcgag
     2401 gctcatcaag gaactgatcg ctatcgattc cggcaacccc tccaacgcgg aactcctcgg
     2461 ctacgtcaaa gacggcgacg gagtgtgcga gaaggcaggc gagttctacc cagtctgcga
     2521 gaagatccag gctgtccggg tcagaacgag gcggcgggat catgccttgc tgttaggcac
     2581 gccaagaacc tcgcgagctt gcgagagaaa caactcgcag cgctccacgg cttgacggac
     2641 ctcttcatca tctcggtaga gcttgaggac cggtaacgtt tcctccaggc ggaacgaagc
     2701 ctcccagagt gcgtcactga cgctctcgcg ctcgccgccg gctttccgcc aagctcgctg
     2761 gaacgttggc tcagaggcgc tacgcagata gcgcagcgac tcgggcgacg agaggatctt
     2821 cgcccacatg gtgagggcgc gggagtcccg cagcacgcgc ggcgcctcag ccgttccgaa
     2881 agtccatcgg acgaattcgc ctagttgctc gatgtggtca cctggaaccg ggtcagaggc
     2941 atcttgaggc gtggctggct ccgacatccc taggaactca cggacaccac cagcttggag
     3001 agcgcgaagt aacacgccaa aggacgtgga catctgcagt gcgccatcga tctctagcgt
     3061 cttggcttgg cgtacaagct catgagccac gtggtgccgt cgcacatgcg atggatagct
     3121 ggagatgcgc gctgctatgt cctcgtaggt gtagccctgc gtaaccaggt gggcaatgta
     3181 gctagccttt tcaagcgcgg gccacggcaa cacgcccgcg acatggcgaa acgctatgta
     3241 ggactcaaca tcggcgtgat ccctcgaagg cgccacgagg acctcgacgg acgaaatcgc
     3301 ctcgcgtacc tcagctgtaa gcccgccagc cagcccggcc cactgaggct tgttctttgg
     3361 ggcgagttcc gggtccaaaa gcagttgcag ggccgcgagc cgacggttgc cttccactat
     3421 gcggaggaca ccctcctcct cgacgcccac catcgggtcc aagggcagcc atcccgattt
     3481 cgcgatcgac tccgccacct cgaacacttt gaacttgtcc accaagatgc ggcggagatc
     3541 gtcctgggaa gaaccctcct cctcgtcggt gagacgtggg ttcctagggt ccagttcggt
     3601 ctgaagcgga tcgacggtcc ggggctcgaa gccctccatg ccacagtcct cctcgagcct
     3661 aagcgcacca cgggagggga ctcccgacgc cttgccccct ctgcttatcg aactgcgctt
     3721 gatcggcgcg tgggttggac gatatcagtc gtggctaggc cgacctctag atatcccgag
     3781 gctggccgtg cgcaccagac ctggtagccc ggtgcgcacg gacaagcatg atggcggcta
     3841 agaacgtggc ggtcagacgt tccccggccc gtcagccgct tggtagccct ctcgtcgaac
     3901 tttggcatga accggaacca cagccatgac gcaaccgaca agcctgccag ggttcgcata
     3961 gtgggaggtc aggtgcacaa cttgccaggg tgcgaaccgg ctcagtcggc cagcagggat
     4021 tcgatctcac tgcgcagaag cggcagcttc gtctcgacga tgtcccacac cagccggtgg
     4081 tccacgtcgg cgtagccgtg gatgaggatg tcgcggaaag cgatgatccg ccgatgctca
     4141 ctcacctggg cggccagatc agcgtccagg cgggcgagct tggacagcgc ttccccgatg
     4201 atctcgaact gccgctcgac cgccgcgcgc aacatcgcgt cggcctgata gtcggcaaag
     4261 gttcgcccag ccacgaactc cgcgagcagc gacgcgccct gctgcatgtc gtagaggtac
     4321 ttcttggcct caagccgcat acagcaacac cttggtccgc tccaccgact cgcggaagaa
     4381 cgggttgcgg atcgccgagg agaccaccag atccacgggc cgtccgaaca gctcctggag
     4441 tgcttccgac aagccgaagt aggcgtcggc atagccgcca ggtggttcag gctggaactc
     4501 gaccagaaag tccagatcgc tgacgccggg ctgatcttct ccgcgggcgg ccgaaccgaa
     4561 taactccaag ctgagaacac caaagcgccg gcagagctca tcgatctctg cctgatgcga
     4621 cgcgagatca ccgttcatcg caccccgctc ctccagccga cagcatctcc ggtcagtgta
     4681 gtggcggggt gtcctcaccc gagcggtatc catctcgccg gaccttcgcg tgaaccgctg
     4741 gcacgacgag tgcggtaccg gccacgccgc cagggttcgc atgctgggag gtcaggtgca
     4801 ccaatgtgcg tgggatgacc ggcttggacc gctcatgtcc cggacgggcc ggcccgtccc
     4861 cgaggagtcc gtcgtcaccg acctgctgtc cgatcttgcg ttgcccgatt acgcgctcgc
     4921 ccggcgagga agtcacgaac tcccgcagcg gtgagccgcc ggcttgacct cgatgccgaa
     4981 cacgcctccg tcgcgacgct ccacgaccac ccaatgcaag cgatgtggga aagtggcatt
     5041 acactatgcg ggaaagcgac atcaacgtct gcgggaaaac ggtatctcac gatagaatgc
     5101 ggggtatggt cccctacctc cccaggctcg tcgacaccct gctggccgaa ctcctcgccg
     5161 agctgccagc ggttcttctc gtgggacctc gtgcggcggg gaagacaacg acggccgccc
     5221 ggcatgccgc aagcctcgtc cgcctcgatc ggcccgccga agcggcagcc ttccgtgccg
     5281 accccgatgc ggcgctggcg agcttcgacg agccggtcgc gctcgacgag tggcaggagg
     5341 ttccgaccgt cctcggcgca gtgaagcgag ccgtcgacgc agacccaaga ccgggccgct
     5401 tcctgctcac cgggtccgtc cgagcggaca tcgaggccga gacctggcca gggacgggcc
     5461 gcgtcgtccg cgtcggcatg ctgcccatga cggtcggcga acgcctcgag cgcacgaccc
     5521 ggccgctcgt tgatcgggtg atggacggcg acgctctcgc gccggcgacg ccggcgacgg
     5581 acctgcctgg ctacatcgcg ctcgcgatgc agagcgggtt tcccgagact ctcggcctgt
     5641 ccgagggcag ccggcgtcga tggatcgaaa gctacgtcga ccagctcctc acgcgggatg
     5701 cgatgctcgt tcacaagggc cgtgacccgg cgcggctgcg gcgctatctg gaggcgtacg
     5761 ccctcaactc ggcagggctc gtcgaggaca agacgatcta cgacgccgcc ggcatcgacc
     5821 gacgcaccgc gctcgcctac gaggcgctgc tcgtgagcct ccaggtcgtc gaggcgctcc
     5881 cggcatggac gtcgaatcgc ctcaagcgcc tgacgctctc gcccaagcgc tacctcgtcg
     5941 atcccgccct cctcgccggg atcatcgggg cgacggagag cgctgtgatg cgtgacggcg
     6001 acctcctcgg tcgtgtcctc gacacctttg tggcggccca tctccgcgcc gaggccgacg
     6061 tcgcgcggca ccgagcccgc ctgtaccacc tgcgtcagga gcaaggccgc cacgagatcg
     6121 acctcctcgc cgagatcggc gccgagcggg tgatcggaat cgaggtcaag gcgaccagcg
     6181 ccccggactc gtcctcggcc gcccatctga cctggctgcg cgagcggctc ggcgaccggt
     6241 tcatcgccgg ggtcgtcctc cacaccgggc cagcgagctt ctccctgggt gatcgcatca
     6301 cggccgcgcc gatcagcacg ctgtgggcct gacatcgtcg cagacttggc gcatacaggg
     6361 ggagggttca acgccgacgg gctcgtgccg agtgcggatg gacccgctac ttcagcgcgc
     6421 accctgcacg ccacggtcat ccacatgcct tgcaggtccg gaggtcgcaa gtctgatccg
     6481 gctcctgccg ggggcaccaa tatttcaagg gtgcgaacct gcgcctggag gaacgatgca
     6541 ccacctgctg gggtgcagtg ccgggacccc cttcttcact gttgcgagaa gtggactcgt
     6601 tcccgcagcc agctggcgaa ccaggcctca tcgacctcgt gtcccgcgac cgcgagcatc
     6661 gcggcttctg cctcgtccac gtcaggggga tggggagccc agtcgccacc gttgagctcg
     6721 atgaacatga ccacacaggc cacgcggcgc gcttgttccc atccggcagc ggatggttcc
     6781 acgccaaccg gcacgtcagc accgcggcct tgtcatgcag ggcagagtcc gcgaggtcga
     6841 cccgcgcggc cttggccaag gtcgaagcgg ctacaccggt gacctgctcg gtccggatag
     6901 aaatcgtcct cgcccatccc tgccgccgcg gcatgacctc cgccgcgtcg acgtgccggt
     6961 gttggtgtca cgcggcgacc agacgccccg gtacttcccg cccgtcgccg aagcgatcgt
     7021 cgaggcgatg ccctcggcgg agggccgtgt cctgaacggt ctcggccatg ctccgcacct
     7081 gacccacccg gagaaattcg tcacgaccgt ggccgacttc ctcacccggt aggcacccgg
     7141 ctcgccgcgc ggctctgcgc gtaccagccg gatcggacca cacggacggt ccgatcggac
     7201 catggcggag atcgttcggc ggcgtaatgt gggctgacat gcccgccgga cggccgggca
     7261 ccgcgccagg ggaacggcgt gacgggggaa tcggaagcgt tcaaggcgcg ccagcgccag
     7321 gtctggtccg tgggcgacta cggcgccatg gccgagcagt accagctcag cgccgaccgc
     7381 ctggtgcagc gcctggagat cggcgccggc atgcgcgtgc tcgacatcgc cacggggacc
     7441 ggcaacgccg ccatcgccgc cgcgatcacc ggcgccgagg tggtcggcat cgacctcacc
     7501 cccgagctct tcgacgaggc gcactggcgc gccgatgagc tggggctggc catcgcctgg
     7561 caggagggtg acgccgaggc gctgccctac cccgacggct ggttcgaccg cgccgtgtcc
     7621 gccttcggga tcatgttcgc cccggacccg cacgcggccg cgcgcgagct cgtgcgcgtg
     7681 ctgcgccccg gcggccgctt cggcatggcg aactggtcgc tgcgcggtcc ggtgctggcg
     7741 gtgttcgaag cggtggccgg cggccaggtc gcgctgccgg cctcggcggc gcaccccatg
     7801 ccctggggcg agccccaggc ggtgcgcacg ttcttcgacg acctgggcgt ggagctcgag
     7861 ttcgcgcacg accacgtgtc gtgggtgttc gccgacgccg atgacggcgt caggtggctg
     7921 gaggaggtct ccggcgcggt catcgccgcc aaggccgtgc tggagccgcg aggcgagtgg
     7981 gcgccggtgc gcgcccgtct ggtcgacgcg gtcacgcagc tgacacggcc ggacatcgaa
     8041 ggcgtctggg tccaggccga gtacctggtg acactgggcc gcaaggcgtg atcaccgccc
     8101 ggtgaagcgg gcctcccggc gctccacgaa gctgcgcagc ccctcggcgg cgtcctggct
     8161 ggccatcacg accggcagca cctcctccag gtggcgccgc tcctcctccg tgacgcgtgc
     8221 cgcgatgcgc gcgctggcca gcgtctcctg cacaccgagc ggcgcctgcg ccgcgatgcg
     8281 ccgcgcgatc tcgacggcgc gctccacgtg gcggccggcg ggcaccacct cctgcaccag
     8341 gccgatgcgg taggcctcct gcgcgccgaa ctcctcggcg gtgagcagaa agcgcatcgc
     8401 gttgccccag ccgagctggc gggtggcgcg gatcgtggcc ccgccgaacg gcaggatgcc
     8461 ccggccgatc tccagctggt tgaagcgcac gtcgtcggcg cacaccacga tgtcggcggc
     8521 cagcgccaac tcgatcgaca gcgtgaacgc gatgccgttg accgccatca ccacgggctt
     8581 gggcaccggg tcgcgccaca ccccgaacgg gtcgtacttg ccccggccgg cgatcgcccg
     8641 cgggccgacg cgcgcgacat cctgggacac ctcggcgagg tccagtccgg cggagaaatg
     8701 gtcgccgtgc gcgaacagca cgcccaccca gcagtccggg tcgtcgccca gcacctcata
     8761 ggcctgcgac aactccgcga tggtctgcag atcgaacgcg ttgcgcttct ccggccggtt
     8821 gaccccgatc agcagcacgt gcccgtcgcg ttccacggtg atgcgctcgc ctgcccagtc
     8881 gctggtcacg tcccgctcct cgtctcgatg ccgacggcgg gcagcctagc ggcggctcag
     8941 gcgaccacga gagcccggtg ctcgggatgc tcctcgatgt aggcggacag gaaggggcag
     9001 gtggggatga tgcgctcgcc ggtgaggcgg agctcgtcga agacggcctc ggcgaactcc
     9061 tcgccgacgc cctgcccgcg ataccgcggc tccaccacgg tgtgctggag gtccagcccc
     9121 ctgtcggtgt gcacataggt cagccgcgcg gcgagctcgt cgccgagata ggcctcgaag
     9181 cgcgacgccg tctcgttgcg cactacccgc agccgcttgc cttcacccat gtcccgctcc
     9241 tcgctcaccg ggtctcctgt cgtccgtgcc aacgccgtcg ccgcaccgac gcttccccgc
     9301 gcccgccgtt ctacgctcca gcgcatggac ctcaacagcg acctcggcga gtccttcggc
     9361 gcgtggcgcc tgggtgagga cgagtcgctg ctggaggtgg tcaccagcgc caatgtggcc
     9421 tgcggcttcc acgccggcga cccggccacg atgcgccgca ccgtggcggc cgccgcggcg
     9481 cgcggcgtgg tgatcggcgc gcaggtgagc taccccgacc tcgtgggctt cggccgccgc
     9541 gagctgggcg ccacgcccga gcagatccac gacgacgtgc tgtaccagct cggcgcgctg
     9601 gacggcatct gccgcgcggc cggcacgcgt gtgcgcttcc tgcgcccgca cggcgccctg
     9661 taccaccgct gcacgcgcga cgtcgccgcc gcggaggcgg cagtggcggc cgcgcgcgcc
     9721 ttcgacgcgg cgctgggggt ggtggcgccg ccggggtcgg ccctgctggc ggcggcggcg
     9781 gcggccgggc tggcgaccgt gcgcgagggc ttcgccgacc gccgctacct ggccgacggc
     9841 acgctggcgc cgcgcggcac ggacggcgcc gtgctcaccg acccggccga ggtcgcggcg
     9901 caagccgtcg cgatcgccac gggggagccg gtccgcgcgg ccgccggcgc accggtcgag
     9961 gtcaccgccg acaccatctg cctgcacggc gacacgcccg gcgccgccgc gctggcgcgc
    10021 gcggtgcgcg cggcgctgga ggcgcacggc gtcccgatcg cgcccgccct ggcatgacgc
    10081 cgcaccccta cggcagccat gcggcattgc tgctcttcga cgacgctgcc gaggtgcagc
    10141 cgtgggtgcg cgccgcgcgt gacgccaacc tgcccggcgt ggtggacgtg gtgcccggcg
    10201 cgcggtccgt ggtgatcgtc ggcgacccgg cgcgcaccga cgccgacgcc ctcatcgccg
    10261 cgctccgcac cctctcccaa cctggaacat tcgtccgaga taccggacca acgttccagg
    10321 tcgggaaggc ggaggtggag gttccggtca cctacgacgg tgaggacctc gccgaggtgg
    10381 cggcacggac cggcctgacc gtggacgagg tcgtcgccgc gcatgccgaa gcggcctacg
    10441 aggtcgcctt ctgcggcttc gcaccgggct tcgcctacct caccggcctg ccggcggccc
    10501 tgcgcctgcc gcgccgcgac acgccgcggc cgagggtgcc cgccgggtcg gtcgccatcg
    10561 ccgccgccta cagcgcggtg tacccgcagg cctcacccgg cggctggtgg ctgctcggcc
    10621 gcaccgccct gacgatgtgg gacgccgacc gggacccgcc cgcgctgctg cagcccggcg
    10681 accgtgtccg cttcgtcgcc acatgatcga gatcgtggcc gcgggggtgc tcgcgctggt
    10741 ggaggacctc ggccggcccg gactggcgcg ctacggcgtc ggaccgtccg gcgcggccga
    10801 ccgcaggtcc catc
//
LOCUS       DATMKC010000060         3086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_159457, whole genome shotgun sequence.
ACCESSION   DATMKC010000060 DATMKC010000000
VERSION     DATMKC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159457"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VNU01_01770"
     CDS             <1..1333
                     /locus_tag="VNU01_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWH31379.1"
                     /translation="FPILERSIRCEDGRERPLVYMDHGASTHAPRPVLEAVMQLMTRH
                     YANVHRGNHTLSQEASDLWDEAILQIAGFVGADPAKQVPIIVGNTTQANDLAAHLMEN
                     VPGRVLTTLMEHHSNDLPYRARGRVLHAGVDEEGRIDLEDVEAKLEQGGVKLLAVSGA
                     SNVTGYMPPIHKMARMAHAHGARILVDGAQLLAHAPIDVKPVGHKEHIDFLSGAGHKA
                     YAPFGAGFLLAPRDIADQAAPYTPGGGTVKWVTAESALFADSPDRHQGGTPNITGTIA
                     LAAALRYLERIGMESVRRHEQELVRQGLKRFAELHDEHGVELLGPHKPKEWKDRLGVF
                     AFLVPEYRHAGVSVRLDRHYAIATRNGCFCAHPLLHRLLRLGDTSQYTKAIAAGQHVE
                     LPGATRATLGLYNTDHEVDVLCDAIGAIAQGKHDGPLRKARVVPRPGQESS"
     gene            1655..>3086
                     /locus_tag="VNU01_01775"
     CDS             1655..>3086
                     /locus_tag="VNU01_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWH31380.1"
                     /translation="MEVFVSSSAPVSKVEVMDDGGAWAAMTYKPEWQSWALSYRIEPG
                     HRVTFRATTAAGVVTSCPFTHPQGVEQCPSSGNPWQQDEYSAISGLGYDLATGDTDGD
                     GRGELYAATELALFEVDQGAVRAVSTLPDWTAVTTGDLDRDGKAEVYGARYSGSASTW
                     EVHRFVRSGSGWSGRIVFAPGSYVSTLTVDDVDRDGQRDLYATGQDSVSRAAAWHLRP
                     TTSGPFVSTRILTVADPWGTAAAEWAWIGDADRDGQVELVVMVAGRDFSEVHMADHTG
                     SWQQVRIGATDDFAGPVVAGDIDGDGKGEVATLGLSLVEVFTFTNGAWSRTVVHETAG
                     IGQDLFLGDGDNDGKQELYILEDRDAWVLRKASGSWSATRIATTPHWALRLIVGDGDR
                     DGIREVFALSFDSDRRLSHITRVQVPATSFDATFSGVRGNEWWVQSMVNGNGQGIAAV
                     DVRLDNGEWRPLARQSWGGWAASYRIV"
BASE COUNT          479 a         1151 c         1054 g          402 t
ORIGIN      
        1 cttcccgatc ctcgagcgca gcatccgctg cgaggacggg cgcgagcggc cgctcgtcta
       61 catggaccac ggcgccagca cgcacgcccc gcgcccggtg ctggaggcgg tcatgcagct
      121 catgacccgc cactacgcca acgtccaccg cgggaaccac accctcagcc aggaggcctc
      181 cgacctgtgg gacgaggcca tcctgcagat cgcgggcttc gtgggcgccg accccgcgaa
      241 gcaggtgccg atcatcgtcg gcaacacgac gcaggccaac gacctcgctg cccacttgat
      301 ggagaacgtg ccggggcgcg tgctcacgac gctgatggag caccacagca acgacctgcc
      361 gtaccgcgcg cgcggccgcg tgctgcatgc cggcgtggac gaggagggtc gcatcgacct
      421 cgaggacgtg gaggccaagc tcgagcaggg cggggtgaag ctgctcgccg tgagcggcgc
      481 gagcaacgtc accggctaca tgccccccat ccacaagatg gcccgcatgg cccacgcgca
      541 cggcgcccgc atcctggtcg acggcgcgca gctgctggcc cacgcgccca tcgacgtcaa
      601 gccggtcggg cacaaggagc acatcgactt cctctccggc gcgggccaca aggcctacgc
      661 gcccttcggc gcgggcttcc tgctggcgcc ccgcgacatc gccgaccagg cggcccccta
      721 cacgccgggc ggaggcaccg tgaagtgggt gacggcggag agcgcgctgt ttgccgactc
      781 gccggaccgc caccagggcg gcaccccgaa catcacggga accatcgcgt tggctgccgc
      841 gctgcggtac ctcgagcgca tcggcatgga gtccgtgcgc cgccacgagc aggaactcgt
      901 gcggcagggg ctgaagcggt tcgctgagct ccacgacgag cacggcgtcg aactgctcgg
      961 cccgcacaag ccgaaggaat ggaaggaccg cctcggcgtc ttcgcgttcc tcgtgcccga
     1021 gtaccggcac gccggcgtct ccgttcgcct cgaccgccac tacgccatcg ccacccgcaa
     1081 cggctgcttc tgcgcccacc cgctgctgca ccgcctgctg cgcctcggcg acacgagcca
     1141 gtacacgaag gcgatcgcgg ccggccagca tgtggagctt cccggcgcca cccgcgcgac
     1201 gctcggcctc tacaacaccg accacgaggt ggacgtgctg tgcgacgcca tcggcgccat
     1261 cgcgcagggc aagcacgacg gccccctgcg caaggcccgc gtcgtgccga ggcccggcca
     1321 ggaatcgagc tgaacccccg ccgacctcga atccaagccc ctctgcgtgg gccgcggcaa
     1381 gcgcaccacc ttgtgcggcg catggacaac cggcgccgtg gtcgaccacg ttcgtccgtg
     1441 ctgcggatat gtcgggcacg ccgttgcggc cgccatgctc cgccccagcc cttcaccaaa
     1501 ccgcctgcaa cgaagctggt cgccagcgtt cgcgggactc accctcgtcc tgctgcttgc
     1561 cgccgcgctt ccgacggacg ctggccaccc gcaggcagca cctggcggcg gctccgtcac
     1621 gttccgccac aaggctggcg gcaacgaatg gtgggtggaa gtgttcgtct ccagctccgc
     1681 ccccgtctcg aaggtggagg tgatggacga cggcggcgcc tgggccgcga tgacgtacaa
     1741 gcccgagtgg cagtcctggg ccctcagcta ccgcatcgag cccggccacc gggtgacgtt
     1801 ccgcgccacc accgctgccg gcgtcgtcac gagctgcccc ttcacgcacc cgcaaggcgt
     1861 ggagcaatgc ccgagcagcg ggaacccgtg gcagcaggac gagtacagcg ccatctcggg
     1921 cctcggctac gacctcgcca ccggggacac cgacggcgac ggccgagggg agctgtacgc
     1981 cgccacggag ctcgcgctct tcgaggtgga ccaaggcgcc gtgcgcgcag tctccaccct
     2041 cccggactgg accgcggtca cgaccggcga cctcgaccgc gacggcaagg ccgaggtgta
     2101 cggcgcccgc tacagcggca gcgcctccac atgggaggtc caccgcttcg tccggtccgg
     2161 ctcggggtgg agcggccgca tcgtgttcgc ccccggctcg tatgtgagca ccctcacggt
     2221 cgacgacgtc gaccgcgacg gccagcgcga cctctacgcc accggccagg acagcgtcag
     2281 ccgcgccgcg gcgtggcacc tgcggccgac cacctccggc ccgttcgttt ccacccgcat
     2341 cctcaccgtg gcggaccctt ggggtaccgc ggcggcggag tgggcgtgga tcggcgacgc
     2401 ggaccgggac ggccaggtgg agctcgtcgt gatggtggcg ggccgggact tctccgaggt
     2461 ccacatggct gaccacacgg ggtcgtggca gcaggtccgc atcggcgcca ccgacgattt
     2521 cgccggaccc gtcgtggcgg gcgacatcga cggcgatggc aagggggagg tggccaccct
     2581 cggcctcagc ctggtggagg tgttcacgtt caccaacggc gcctggtccc gcaccgtggt
     2641 gcatgagacg gccggcatcg ggcaggacct gttcctgggc gacggcgaca acgacggcaa
     2701 gcaggagctg tacatcctgg aggaccgcga cgcgtgggtg ctgcgcaagg cctccggcag
     2761 ctggagcgcc acccgcatcg cgacgacgcc gcactgggcg ctgcgcctca tcgtcggcga
     2821 cggcgaccgc gacggcatcc gcgaggtgtt cgcgctttcc ttcgacagcg accggcggct
     2881 gtcccacatc acccgcgtgc aggtgccggc cacgagcttc gacgcgacct tctcgggcgt
     2941 acgcggcaac gagtggtggg tgcagtcgat ggtcaacggc aacggccagg gcatcgcggc
     3001 cgtcgacgtc cgcctggaca acggcgaatg gaggcccctg gcccgccaat cctggggcgg
     3061 ctgggcggcc tcctaccgga tcgtgg
//
LOCUS       DATMKC010000061         4678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_42620, whole genome shotgun sequence.
ACCESSION   DATMKC010000061 DATMKC010000000
VERSION     DATMKC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42620"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VNU01_01780"
     CDS             <1..230
                     /locus_tag="VNU01_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31381.1"
                     /translation="NGASHPATTTYLGNVGWITSAGGTLPTTGDVLDGPGGNPIGTLV
                     GVTQAQGGTACAAAPGAQTFAVVGASALLPL"
     gene            415..4362
                     /locus_tag="VNU01_01785"
     CDS             415..4362
                     /locus_tag="VNU01_01785"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HWH31382.1"
                     /translation="MVFQSRRVVRSRIVGLAALAAVLMGALPAAASVAPPEDANGSPA
                     VALQQERLRVPDHAHPEWPGEGARQGGARTMWAWSEGRRGYFLGGLGALHGYATVSSV
                     DLDSGEEVARATLSPAALGTGANMAGHAHIATVDEQNGRLLVAYEVPPASRLVEETSD
                     TQGGLGRQGDRLVPVGTYPEGQCTHLEGGAPGSAKDEAHKLCMRYLDKEPGAVAVPKE
                     PEPELILPSGVGLEQCLSHHTECFGGITVLDGETLDPLRTIQYQPVRVDGGAVVPFLR
                     ALSFEPASQAGDDTVPAKVYAIVEEATGFVGDPRMTRTHVSNVVYVVQFDPETGQQDW
                     ALRIDQCRGARESRSDGTNLSELRNLPHAATVYRTTRGEQPVMVVGCHSGGTQVGTVV
                     RVPLTTASVPQPPAAAPGTGAVEDVDLEAGPAALGGLAPQTSQAQSYAGPDKVWEMLA
                     DPASDRVLMKVVDGNPQAEVWWVFDGGEGRFVGTIGIGLYSKDQTVSALDTERGRLYV
                     RAPTTPLAGGALYVADVRRTPLSQALVFPALELPSGGGQMTVDTGGQERPTRLLAKAG
                     DVYQVIVDRLPVSVDLPVDDFAGRTLDLAEVEGVTASTFDAVARGYGMRALFVGGLEA
                     ATRAGPADPAGQAFGAQDVALGTGPFQPGGPGHGLTRDGTLPLTSPCTAGNREVVLGM
                     VGPESPAVVDSSGGRAAAEPVIVDTGLRADSEAPASRCLPIEWNAVWRAALFNNAPVA
                     EPTTRWAFGNGEASCVSTNEDAQGAFGDAVTGGFAASVECQDEQVSGWSYARAMTLEG
                     GVAVGEASSRFTVYRDPGRGVVSRVESIARAVNVPGAFSIDVVRSVAESWANGRRQPV
                     DAKDRPADYDPNCDLERSAGTCFVRQIMGVRSPHYSCTQCSDQRALVEGLNSAFGSQG
                     TVQLRRPEARLAKGAENGFAAAVKKPDAEQFADLVLNNDLLQTVLPGLEIVRYAQPNR
                     LYGGGQRGRQIYQFAGVEVSSSYGISCLLTYDAETNTCDVAKEPPGSLTITLANGKGE
                     PLAGGAFEVRADSDGDGILGLADALLPDGACVTADDGVGTCVFDDVAPGTYHVTQVAA
                     PPGYTKLDEPAAVAVASGEAATLAVTNLTNVAVIEVKASDEAGNPVAGAVFAAYPDPD
                     ADGKVAADAQAAGTCTTDADGGCEIRVPSGSYVLVQTSAPGGLEGIAPVAFAVTGGQT
                     AAVGVVNYPPAMPAAPEPAPVAYEPSPAMPEPVVSSGPYAPSEPVGEVAEPEVSLPEA
                     VGGTVVRVIRAPGDAVRLLARDPMQAVAWIASIALFCLAVLAVRRRIAGLRLQ"
     gene            complement(4374..4545)
                     /locus_tag="VNU01_01790"
                     /pseudo
     CDS             complement(4374..4545)
                     /locus_tag="VNU01_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558666.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          617 a         1635 c         1817 g          609 t
ORIGIN      
        1 ccaacggcgc gtcccacccg gccacaacca cctacctcgg caacgtcggc tggatcacct
       61 cggccggcgg caccctgccg accaccggtg acgtgctcga cggccccggc ggcaacccca
      121 tcggcacgct cgtgggcgtg acccaggcgc agggcggcac ggcgtgcgcc gccgctccgg
      181 gcgcgcagac cttcgcggtc gtcggcgcct cggcgctgct gccgctgtaa gcggacggca
      241 tcaaatcggg ggcgggctgc ggcccgcccc cgacccgtct ccgtgccggg cgcgcaccgc
      301 ttcccgcgga gcgggagacg cccgtaaggc gatggcacag agattcgtat taacgaataa
      361 gagtcgggat cgaggagcgg gatcggcgga cccgccccgg cagccggagg agagatggtg
      421 ttccagtcac gccgtgtcgt gagatcccgc atcgtggggc tcgccgccct ggccgccgtg
      481 ctcatgggtg cgctgccggc cgccgcgtcg gtggcaccgc cggaggacgc aaatggatcg
      541 cccgccgtcg cgctccagca ggagcgcctc cgggtgccgg accacgcgca cccggagtgg
      601 ccgggcgagg gcgctcgcca gggcggcgcg cgcaccatgt gggcgtggtc ggagggccgg
      661 cgcggctact tcctgggcgg cctcggcgcg ctgcacggct acgccacggt ctcctcggtc
      721 gacctggaca gcggtgagga ggtcgcccgc gccacgctga gccccgccgc gctggggacc
      781 ggcgcgaaca tggccggcca cgcccacatc gccacggtgg acgagcagaa cggccggctg
      841 ctggtggcct acgaggtgcc gcccgcgtcg cgcctcgtgg aggagaccag cgacacgcag
      901 ggcggcctgg gccgccaggg cgaccggctg gtcccggtgg gcacctaccc ggagggccag
      961 tgcacgcacc tggagggcgg cgcgccgggc tctgccaagg acgaagccca caagctctgc
     1021 atgcgctacc tggacaaaga gccgggcgcg gtggccgtgc ccaaggagcc agagccggag
     1081 ctcatcctgc caagtggtgt cggcctggag cagtgcctca gccaccacac ggagtgtttc
     1141 ggcggcatca cggtcctgga cggcgagacg ctggacccgc tgcgtaccat ccagtaccag
     1201 ccggtgcgcg tggacggggg cgcggtggtg ccgttcctgc gcgcgctatc gttcgagccg
     1261 gcctcgcagg ccggggacga caccgtcccg gcgaaggtct acgccatcgt ggaggaggcg
     1321 acgggcttcg tcggcgaccc gcgcatgacc aggacccacg tcagcaacgt ggtgtacgtg
     1381 gtgcagttcg acccggagac gggccagcag gactgggcgc tgcgcatcga ccagtgccgc
     1441 ggcgcgcgcg agtcgcgctc cgacggcacc aacctgagcg agctgcgcaa cctgccgcac
     1501 gccgccacgg tgtaccgcac gacgcgcggc gagcagccgg tcatggtggt gggctgccac
     1561 agcggcggca cccaggtggg caccgtggtg cgcgtgcccc tgaccacggc cagcgtcccg
     1621 cagcctccgg cggccgcgcc cggcaccggg gccgtggagg acgtcgacct cgaagccggc
     1681 ccggcggcgc tgggcggcct ggcgccgcag accagccagg cgcagtccta cgccggaccg
     1741 gacaaggtct gggagatgct ggccgacccc gcgtccgacc gcgtgctgat gaaggtcgtc
     1801 gacggcaacc cgcaggccga ggtgtggtgg gtgttcgacg gcggcgaggg ccgcttcgtc
     1861 ggcaccatcg gcatcggcct gtacagcaag gaccagaccg tctccgcgct ggacaccgag
     1921 cgcggcaggc tctacgtgcg cgcgccgacg acgcccctgg ccggcggtgc gctctacgtc
     1981 gccgacgtcc gccgcacgcc gctgtcccag gcgctggtgt tccccgcgct ggagctgccc
     2041 tccggcggtg gccagatgac cgtcgacacc ggcgggcagg agcggccgac gcgcctgctg
     2101 gccaaggccg gcgacgtcta ccaggtcatc gtggaccggc tgcccgtgtc cgtggacctg
     2161 ccggtggacg acttcgcggg ccggacgctg gacctggccg aggtcgaggg cgtgacggcg
     2221 tcgacgttcg acgcggtggc gcgcggctac gggatgcgcg cgctgttcgt gggcggcctc
     2281 gaggcggcca cgcgcgccgg gcccgccgac cccgcggggc aggcgttcgg cgcgcaggac
     2341 gtggcgctgg gaaccggccc gttccagccc ggcgggcccg ggcacggctt gacccgcgac
     2401 ggcaccctgc cgctgaccag cccgtgcacg gcgggcaacc gcgaggtggt cctgggcatg
     2461 gtggggccgg agtcgcccgc cgtggtggac agctccgggg gccgcgccgc ggccgagccc
     2521 gtcatcgtgg acaccggcct gcgcgccgac agcgaggcgc cggcgtcgcg ctgcctgccg
     2581 atcgagtgga acgcggtgtg gcgcgcggcg ctgttcaaca acgcgccggt ggcggagccg
     2641 accacgcgct gggcgttcgg caacggcgag gcgtcctgcg tgtccaccaa cgaggacgcg
     2701 cagggggcgt tcggcgacgc ggtgacgggg ggtttcgccg ccagcgtgga gtgccaggac
     2761 gagcaggtca gcggctggag ctacgcccgc gccatgacgc tggagggcgg cgtggcggtg
     2821 ggcgaggcct cgtcgcgctt caccgtgtac cgcgacccgg gtcgcggcgt ggtctcgcgc
     2881 gtggagtcga tcgcgcgcgc cgtcaacgtg ccgggcgcgt tcagcatcga cgtggtgcgg
     2941 tcggtggccg agtcctgggc caacggccgg cgccagcccg tggacgcgaa ggaccggccc
     3001 gcggactacg accccaactg cgacctggag cgcagcgccg ggacctgctt cgtgcgccag
     3061 atcatggggg tgcgcagccc gcactactcc tgcacgcagt gcagtgacca gcgcgcgctg
     3121 gtggagggcc tgaacagcgc cttcggctcg cagggcacgg tgcagttgcg ccggccggag
     3181 gcgcgcctgg ccaagggcgc cgagaacggc ttcgccgccg cggtgaagaa gccggacgcg
     3241 gagcagttcg ccgacctggt gctcaacaac gacctgctgc agacggtgct gccgggcctg
     3301 gagatcgtgc gctacgccca gcccaaccgc ctgtacgggg gcggccagcg cggccggcag
     3361 atctaccagt tcgccggcgt ggaggtgtcc tcgtcctacg ggatcagctg cctgctgacc
     3421 tacgacgcgg agaccaacac ctgcgacgtg gccaaggagc cgccggggtc gctgacgatc
     3481 acgctggcca acggcaaggg cgagcccctg gccggcggtg ccttcgaggt gcgcgccgac
     3541 tccgacggcg acggcatcct gggcctggcc gacgcgctgc tgccggacgg cgcgtgcgtc
     3601 accgccgatg acggcgtggg cacgtgcgtg ttcgacgacg tggcgcccgg cacctatcac
     3661 gtgacgcagg tggcggcgcc gccggggtac acgaagctgg acgagccggc cgcggtcgcg
     3721 gtcgcctccg gcgaggcggc gacgctggcg gtgaccaacc tcaccaacgt cgcggtgatc
     3781 gaggtgaagg cgtccgacga ggccggcaac ccggtggcgg gcgcggtgtt cgccgcctac
     3841 ccggatcccg acgcggacgg caaggtggcc gcggacgcgc aggcggcggg cacctgcacc
     3901 acggacgccg acggcgggtg cgagatccgt gtcccgtcag gcagctacgt gctggtgcag
     3961 acctccgcgc ccggcggctt ggagggcatc gcgccggtgg cgttcgccgt gaccggcggc
     4021 cagaccgctg cggtcggcgt ggtgaactac ccgccggcga tgcccgcggc gcccgagccg
     4081 gcgccggtgg cctacgagcc ctctccggcg atgccggaac cggtggtctc ctccgggccg
     4141 tacgcgccct cggagccggt cggcgaggtg gcggagcccg aggtcagcct gccggaggcg
     4201 gtgggcggca cggtggtccg cgtcatccgc gcgcctggtg atgccgtgcg gctgctggcc
     4261 cgcgacccga tgcaggccgt cgcctggatc gcctccatcg ccttgttctg cctggcggtc
     4321 ctggcggtcc gccgccgcat cgcgggattg cggctgcagt agcgccgggg cgtctagctg
     4381 tagtgaccag gcaggttgtt gacgcgctgg ataggtgcgt ggccgccgag tgcggtgtgg
     4441 ctgcggtgat ggttgtagat gtgtagccag tcggcaaggg cgccgctgcg ttggtgctct
     4501 gagctgtagc cgaacgtagg cccattcctc aagcagagtt gggcttcccc cggttctccg
     4561 gagtcggtgg gggtggaatt ctcatgccac gacgtgtagg cgtcggttct cgtattcgat
     4621 gggtgtgagc atgccaagcg cggagtggcg gcgctggcgg ttgtggaaga tctcgagg
//
LOCUS       DATMKC010000062         3396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_88443, whole genome shotgun sequence.
ACCESSION   DATMKC010000062 DATMKC010000000
VERSION     DATMKC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88443"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1174)
                     /locus_tag="VNU01_01795"
     CDS             complement(317..1174)
                     /locus_tag="VNU01_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWH31383.1"
                     /translation="MAYEYEHFLVDVDDVGVATVTINRPDKMNAFTYDMERDIAQIMW
                     ALDADDDVRVIVLTGAGRAFSSGIDLSQGPQVFSEEGKDEHAGTTGGSSESLPEKFGW
                     WRMATPVIAAINGAAIGAGLTLTLLADLRVVADDAKLQFVFSRRAMLPEANSTWLLAR
                     LIGASRAMELLLTGRSFDGQEAREYGIAVRSVPADQVLATAQELARDMAVQCSPATLG
                     LIKRVLYHFMSETDRSKAMVQETDLVWWMGTQPDIVAGIMAWMSNKDPQWKMPKYPDV
                     PEHLRFYEE"
     gene            complement(1231..2247)
                     /locus_tag="VNU01_01800"
     CDS             complement(1231..2247)
                     /locus_tag="VNU01_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWH31384.1"
                     /translation="MKLGVTLPYAEGEMTRAQMVEFVQAADRLGYDTVWVPEAWTYDA
                     FMLLASVAELTEHIKLGTSIVNVYSRTPALIGQSTATLDMLSGGRAVLGIGASGPQVI
                     EGWHGMPYRKPLQRTRETIDIVRMILRRDRLNYDGDVFQLGMGLKLINHPLRPDVPIY
                     VASLGPKNVAMTAELADGWIPVLFSPTRWREVFGDALDEGLAKRSADLPPLQITPSVS
                     IGITDAPQAAATIARFGLALYIGGMGSREHNFYNEVARRYGYVDEAAEIQDLFLGGDK
                     GAAAERVTDAMVDEFSVIGPEGLVKEKLSAFKESGASALLVNVIAMSQRQRLEALEKI
                     IGLV"
     gene            complement(2294..>3396)
                     /locus_tag="VNU01_01805"
     CDS             complement(2294..>3396)
                     /locus_tag="VNU01_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31385.1"
                     /translation="ARETQRVLAEDGWAAPQWPREFGGRGCSLVELMIWAEEKAAFHL
                     PVGVFEIGLAMCGPTIMAHGTDEQRARYLPPLLRGDEIWCQLWSEPGAGSDLAGLSSR
                     AERDDSGDWVLNGQKVWTSGAHYAQFGLGVFRSNPDVPKHKGITCLIVDMSAPGVEVR
                     PLRQITGDAHFNEVFFTDAVVPAANVVGEVDGGWGVARTLMMFERAAVGGMTSPSRAI
                     APLLRLARRTVRDGGTAAEHAVLRQRLAELYIAGRLFDLTTARVRSALSKGGMPGTES
                     SILKLAAAQLGTRAAETGLDVLGAGGALVGPDAPEGGRWADALLGAPALHIGGGTDNI
                     QRNIIGEQVLGLPREPSTDRDVPFRDLPVAGR"
BASE COUNT          484 a         1242 c         1197 g          473 t
ORIGIN      
        1 caccttgaca acaacataga gaaaccagcg ctcgcccata ccggctgacc cgccacccca
       61 cccgccaggt ggggcgcgaa gcccgcccac acaccgcgac atggcaagaa cgggcgggca
      121 tcacggccgc gcgggcatgg cgtcgcgcgg gacgtggcgg tcgcggggga accggagctc
      181 gacgaggacg agcccggccc tcggcaccga acacgtgcgg ccgcaagatg gccgccgacg
      241 cccgacaccg cgatatcagc cgggcgagga gcgaggaggg gtccggaccc acgcgaccac
      301 cggcgccggg caggtgctac tcctcgtaga agcgcaggtg ctcaggcacg tccgggtact
      361 tgggcatctt ccactgcggg tccttgttgg acatccaggc catgatgccg gccacgatgt
      421 cgggctgcgt gcccatccac cagaccaggt ccgtctcctg gaccatcgcc ttggagcggt
      481 cggtctcgga catgaagtgg tagagcacgc gcttgatgag gcccagcgtg gcgggcgagc
      541 actggaccgc catgtcgcgc gcgagctcct gcgcggtggc cagcacctgg tcggcgggca
      601 cggagcgcac ggcgatgccg tactcgcggg cctcctggcc gtcgaagctc ctgccggtca
      661 gcagcagctc catggcgcgg gaggcgccga tgaggcgggc gagcagccag gtggagttcg
      721 cctcgggcag catggcgcgg cgcgagaaca cgaactgcag cttggcgtcg tccgcgacga
      781 cgcgcaggtc cgcgagcagc gtgagcgtga gccccgcgcc gatggcggcg ccgttgatcg
      841 cggcgatcac gggcgtggcc atgcgccacc agccgaactt ctccggcagc gactccgacg
      901 agccgccggt ggtgccggcg tgctcgtcct tgccctcctc ggagaagacc tgcggcccct
      961 gggagaggtc gatgccgctg gagaacgcgc ggccggcgcc ggtgagcacg atgacgcgca
     1021 cgtcgtcgtc ggcgtccagg gcccacatga tctgggcgat gtcgcgctcc atgtcgtagg
     1081 tgaacgcgtt catcttgtcc ggacggttga tcgtgaccgt cgcgacgccg acgtcgtcga
     1141 cgtcgaccag gaagtgctcg tactcgtacg ccatgccgat gcctctcgtc cgccgggcgc
     1201 cgcgcgccct gtgcgcttgt ccctgccgcc tcagacgagg ccgatgatct tctccagcgc
     1261 ctcgaggcgc tgccgctgcg acatcgcgat gacgttcacc agcagggccg acgccccgga
     1321 ctccttgaac gccgagagct tctccttcac caggccctcg ggaccgatca cggagaactc
     1381 gtcgaccatc gcgtcggtga cccgctccgc cgcggcgccc ttgtcgccgc ccaggaagag
     1441 gtcctggatc tcggcggcct cgtccacgta cccgtagcgc cgcgcgacct cgttgtagaa
     1501 gttgtgctcg cgggagccca tgccgccgat gtagagggcg agcccgaagc gggcgatggt
     1561 cgccgcggcc tgcggcgcgt cggtgatgcc gatgctcacc gacggggtga tctgcagcgg
     1621 cggcaggtcg gcggagcgct tcgccagtcc ctcgtcgagg gcgtcgccga acacctcgcg
     1681 ccagcgggtg ggcgagaaca gcaccgggat ccagccgtcg gcgagttccg cggtcatcgc
     1741 cacgttcttc gggccgagcg aggccacgta gatcggcacg tcgggccgca gcgggtggtt
     1801 gatcagcttg agccccatgc ccagctggaa gacgtcgccg tcgtagttca gccggtcgcg
     1861 gcgcaggatc attcgcacga tgtcgatggt ctcgcgggtg cgctggagcg gcttgcggta
     1921 cggcatgccg tgccagccct cgatcacctg cgggccggac gcgccgatgc ccagcaccgc
     1981 tcgcccgccg gacagcatgt cgagcgtggc ggtggactgc ccgatcagcg caggcgtgcg
     2041 cgagtagacg ttgacgatgc tggtcccgag cttgatgtgc tcggtcagct cggcgacgga
     2101 cgcgagcagc atgaacgcgt cgtaggtcca cgcctccggg acccacacgg tgtcgtaacc
     2161 caggcggtcc gccgcctgca cgaactccac catctgggcg cgggtcatct cgccctcggc
     2221 gtacggcagc gtcactccca gcttcatgcc ttgctccctc gtcggaatcg gtcccgcctg
     2281 cccggcggcg cgctcaccgg cccgccaccg gcaggtcgcg gaacgggacg tcgcggtcgg
     2341 tggacggctc gcggggcagg ccgagcacct gctcgccgat gatgttgcgc tggatgttgt
     2401 cggtgccgcc gccgatgtgc agcgccgggg cgcccagcag cgcgtccgcc cagcgcccgc
     2461 cctccggggc gtccggcccc acgagcgcgc cgccggcgcc gagcacgtcc aggccggtct
     2521 cggcggcccg ggtgcccagc tgcgcggcgg cgagcttcag gatcgagctc tccgtgcccg
     2581 gcatgccgcc cttggacagc gcgctgcgga cccgggcggt agtgaggtcg aacagccggc
     2641 ccgcgatgta cagctccgcc aggcgctggc gcagcacggc gtgctcggcg gccgtcccgc
     2701 cgtcgcgcac cgtgcgccgc gccagccgca gcagcggcgc gatcgcgcgg ctgggcgacg
     2761 tcatgccgcc gacggcggcc cgctcgaaca tcatcagcgt gcgggccacg ccccagccgc
     2821 cgtcgacctc gccgacgacg ttggccgcgg gcaccaccgc gtcggtgaag aacacctcgt
     2881 tgaagtgcgc gtcgccggtg atctgacgca gcggccgcac ctccacgccc ggcgcggaca
     2941 tgtccacgat caggcaggtg atgcccttgt gcttcggcac gtccgggttg gagcggaaca
     3001 cgcccaagcc gaactgcgcg tagtgggcgc cgctggtcca caccttctgg ccgttcagca
     3061 cccagtcccc ggagtcgtcg cgctcggccc ggctggacag cccggcgagg tccgagccgg
     3121 cgcccggctc cgaccacagc tggcaccaga tctcatcgcc ccgcagcagc ggcggcaggt
     3181 agcgcgcgcg ctgctcgtcc gtgccgtgcg ccatgatcgt cggcccgcac atcgccaggc
     3241 cgatctcgaa cacgccgacg ggcaggtgga aggcggcctt ctcctcggcc cagatcatca
     3301 gctcgaccag cgagcagccg cggccgccga actcccgggg ccactgcggc gccgcccagc
     3361 cgtcctcggc gagcacgcgc tgcgtctcgc gcgcct
//
LOCUS       DATMKC010000063         5529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_207661, whole genome shotgun sequence.
ACCESSION   DATMKC010000063 DATMKC010000000
VERSION     DATMKC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207661"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VNU01_01810"
     CDS             complement(<1..187)
                     /locus_tag="VNU01_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWH31386.1"
                     /translation="MKKCPQCAEEIQDEAVVCRYCGARFDGVPPAPAGAPSAQTTNGF
                     AVASLVLGIVWVWWIGSV"
     gene            complement(240..953)
                     /gene="lipB"
                     /locus_tag="VNU01_01815"
     CDS             complement(240..953)
                     /gene="lipB"
                     /locus_tag="VNU01_01815"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWH31387.1"
                     /translation="MNELGRPPLLWLPAGRVPYEHALAWMRGLVELRHAGAIPDVVLT
                     LEHPPVYTAGRHADVDAHVLPAGAPGSRADIPLVRTDRGGDVTYHGPGQLVAYPVVRL
                     RDGKSARRFVRALERAVIAVLAGYGLEGVPGEDLHRPGVWVGGDKVAAVGVRVSGGVS
                     SHGLALNVDPDLAGFGGIVPCGIADAGVCSLASLGVAATTAEVAERLVPALGEALGRA
                     ARRATGDEVPAPPGIPLLG"
     gene            complement(967..2529)
                     /gene="sucB"
                     /locus_tag="VNU01_01820"
     CDS             complement(967..2529)
                     /gene="sucB"
                     /locus_tag="VNU01_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HWH31388.1"
                     /translation="MSERQRRRRGGAPQEPAQEPASTRRRRRGGPPQAATEHSGRRRR
                     GGTGNAAETPAVAPQEPAQPPVEDRAPRTTQSEPEPRAPERSTTSVATDVTLPQLGES
                     VTEGTITAWLVNVGDHVEADQPLFELSSDKIDTEVPAPASGVLSEILVNVDETVEVGT
                     VVARIGSDGAAGASPDGDGEAPQPAAKAEEPPAEAEPEPAGDKTATPSQRPPLTSPLA
                     RREATQAGVDVDAIQGSGQGGRVTREDVQAAAGGRGAPQPAAAQAKPAASPQPARSVP
                     LSAGDRERLEDLSRIRQRIAAKMTESMQSAAQLTTVQEADVTRIMRLRGKVKQQFAER
                     EGVSLSPFVFLARAAVIAIKRHELANAQADWQAGKVRIPEYVNLGIAVDTEKGLLVPN
                     VKNADDLTLPALARAINEAAAKARGELKLEMHDIEGGTFTVTNTGSRGVLLDTPILNY
                     PEVAILATGAIQKRPVVVDTDTGPSIAIRDMVYLCLTYDHRLLDGADAARFVTDIKQV
                     IETHDWESEIGY"
     gene            complement(2582..4003)
                     /gene="lpdA"
                     /locus_tag="VNU01_01825"
     CDS             complement(2582..4003)
                     /gene="lpdA"
                     /locus_tag="VNU01_01825"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWH31389.1"
                     /translation="MPDTFDLLVLGGGTGGYSTALRAAGIGMKVALVEKAKVGGTCLH
                     WGCIPTKALLQAAEVAEHAAHAAEYGVKATFEGIDVPAVQQYKDTIVDTNWKGLQATM
                     KARGIETVAGTGRVSDPRTLVVQTDEGERTLQAAKGLVLATGSVPREIPVEGAEVDGE
                     TVITSDHALFLDRVPTRPIILGAGAVGVEFATVWNAYGAEKVTVVEMLPGLIPNEDAD
                     SQKALAKEFKKAGIDALVNTKVTKVEKAGDGVRVTVQDAKGGESQLEGDVLMIAVGRR
                     PVTEDMGFEEAGVTVDRGFVPVDAYLRPGPSWDGGAVYAVGDILPPPSLGLAHASFQE
                     GFLVAEHAAGMAVTPIDYAGIPRVTYCHPEVASVGHNEQALKEQGVAYDKKLFPFSHN
                     GRAMMMKAGGHVKVLAAKDGGKVLGVHIVGPRATDLIAEAELIYNWEALPLEVAAFIH
                     PHPTLSEAVGEAHMALAGKALHG"
     gene            4118..5194
                     /gene="gcvT"
                     /locus_tag="VNU01_01830"
     CDS             4118..5194
                     /gene="gcvT"
                     /locus_tag="VNU01_01830"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWH31390.1"
                     /translation="MGERTGPLHERHVALGAAMTDFGGWLMPLHYGSVLAEHRAVRED
                     CGVFDVSHLGRLLVRGDGADAAVSAVLTNDVAALAPGRAQYSLCLDEAGGIVDDVLVY
                     RLTGGWSDGLLVVPNAANAAAVAERLRHAAPAGTQVRDVQDELACLAVQGPRSAEVVE
                     AIGIPVARYGYLDCFDMAGAGVVARSGYTGERGYELFCLAEHAPVVWDRLLAAGAAPA
                     GLGARDTLRLEMGYPLHGSDIGPGTSPVEAGLTWAVKGEHDFTGRAAYEAAKAAGPRR
                     RLRGVRVEGRGIPRAHCAVWRGGERVGELTSGTFSPTLGVGIGMGYLAAGVDVGARVE
                     IEVRGKRLPAAVVAPPFVDRNPRT"
     gene            complement(5198..>5529)
                     /locus_tag="VNU01_01835"
     CDS             complement(5198..>5529)
                     /locus_tag="VNU01_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31391.1"
                     /translation="DGDLGTPLHPSMPRGGEPPPPPPAPGEGGREESVVLDLGGAAPR
                     SLVVLRGEPSEMDVELSADGVRWTPPVRTQSHGPATAVSVQGDARYVRVRTTMGTITR
                     LVEVSVW"
BASE COUNT          617 a         2062 c         2064 g          786 t
ORIGIN      
        1 gcaccgagcc gatccaccac acccacacga tgcccaggac cagggacgcg actgcgaagc
       61 cgttggtggt ctgcgccgaa ggcgcaccgg ccggggccgg cggcacgccg tcgaaccgcg
      121 ccccgcagta gcggcacacg accgcctcgt cctggatctc ctccgcgcac tgcgggcact
      181 tcttcatccg gctctcccca tcgggctctg cgtgccgcct gtggccgtcg ccgccaacac
      241 tagcccagca gcgggatgcc gggcggcgcc gggacctcgt cgccggtggc gcggcgggcg
      301 gcccggccga gggcttcgcc cagcgccggc accagccgct cggccacctc cgcggtggtg
      361 gcggccacgc ccagcgaggc gagcgagcac acgcccgcgt cggcgatgcc gcacggcacg
      421 atgccgccga agccggccag gtccgggtcg acgttcagcg ccagcccgtg gctgctgacc
      481 ccgccggaca cgcgcacgcc gacggccgcg accttgtcgc cgcccaccca cacgcccggg
      541 cggtgcaggt cctcgccggg caccccctcc agcccgtagc cggcgagcac cgcgatgacc
      601 gcgcgctcca gcgctcgcac gaagcgccgc gcggacttgc cgtcgcgcag gcgcacgacc
      661 gggtaggcca caagctggcc cggaccgtga taggtgacgt cgccgccgcg gtcggtgcgc
      721 accagcggga tgtccgcgcg cgagccgggc gcgccggccg gcagcacgtg cgcgtcgacg
      781 tcggcgtggc ggcccgccgt gtagaccggc gggtgctcca gggtgagcac cacgtcgggg
      841 atcgcgccgg cgtggcgcaa ctccaccagc ccgcgcatcc acgcgagcgc gtgctcgtag
      901 ggcacgcggc cggccggcag ccacagcagc gggggccggc cgagctcgtt cacgggcgcg
      961 gaccggctag tagccgatct cggactccca gtcgtgcgtc tcgatgacct gcttgatgtc
     1021 ggtcacgaag cgcgcggcgt cggcgccgtc gagcagccgg tggtcgtagg ttaggcacag
     1081 gtacaccatg tcgcggatcg cgatggacgg accggtgtcg gtgtccacca ccacggggcg
     1141 cttctggatc gcgccggtgg ccaggatggc gacctccggg tagttgagga tcggcgtgtc
     1201 cagcagcacg ccgcggctgc cggtgttggt cacggtgaac gtgccgcctt cgatgtcgtg
     1261 catctccagc ttgagctctc cgcgcgcctt ggcggccgcc tcgttgatcg cgcgcgccag
     1321 cgcgggcagc gtgaggtcgt cggcgttctt cacgttgggg accagcaggc ccttctccgt
     1381 gtccacggcg atgcccaggt tcacgtactc cgggatgcgc accttgccgg cctgccagtc
     1441 cgcctgcgcg ttggccagct cgtgacgctt gatggcgatg accgccgcgc gcgccaggaa
     1501 cacgaacggc gacaggctca cgccctcgcg ctcggcgaac tgctgcttga ccttgccgcg
     1561 cagccgcatg atgcgggtga cgtcggcctc ctgcaccgtc gtgagctgcg ccgcggactg
     1621 catggactcg gtcatcttcg cggcgatgcg ctggcggatg cgcgacaggt cctccaggcg
     1681 ctcgcggtcg cccgcgctca gcgggacgct gcgcgcaggc tgcggcgagg cggccggctt
     1741 ggcctgcgcg gcggccggct gtggggcgcc gcggccgccg gcggccgcct gcacgtcctc
     1801 ccgggtgacc cggccgccct ggccgctgcc ctggatcgcg tcgacgtcga caccggcctg
     1861 cgtggcctcg cggcgtgcca gcggggaggt cagcggcggc cgctgcgatg gcgtggcggt
     1921 cttgtcgccc gcgggctcgg gctccgcctc ggcgggcggc tcctcggcct tcgcggccgg
     1981 ctgcggtgcc tcgccgtcgc cgtccggcga cgcgccggcg gcgccgtccg agccgatgcg
     2041 cgcgacgacg gtgccgacct cgaccgtctc gtccacgttc accaggatct cgctgagcac
     2101 ccccgacgcg ggcgcgggga cctcggtgtc gatcttgtcc gagctcagct cgaacagcgg
     2161 ctggtccgcc tcgacgtggt cgcccacgtt cacgagccat gccgtgatcg tgccctcggt
     2221 gacggactcg ccgagctggg gaagggtgac gtcggtggcc acgctcgtcg tgctcctctc
     2281 cggagcccgc ggctccggct cgctctgggt ggtgcgcggt gcccggtcct cgaccggggg
     2341 ctgtgcaggc tcctgtggtg caaccgccgg cgtctccgcc gcgttcccgg tgcccccgcg
     2401 acgccgccgt ccggagtgct ccgtcgcggc ctgcggcggg ccgccgcgcc gccggcgtcg
     2461 cgtcgacgcc ggctcctgtg ccggctcctg cggcgcgccg cctctccggc gtcgctggcg
     2521 ctcgctcatc gaatggtctt ccctcgcgtg tggtcggaca accttggtgc gcggcggacg
     2581 cctagccgtg cagcgccttg cccgccagcg ccatgtgcgc ctcgccgacg gcctcggaca
     2641 gggtcgggtg cgggtggatg aaggcggcca cctccagcgg cagcgcctcc cagttgtaga
     2701 tcagctccgc ctcggcgatg aggtcggtgg cccgcgggcc cacgatgtgc acgcccagca
     2761 ccttcccgcc gtccttggcc gccagcacct tcacatggcc gccggccttc atcatcatcg
     2821 cgcggccgtt gtggctgaag gggaacagct tcttgtcgta ggcgacgccc tgctccttca
     2881 gcgcctgctc gttgtggccg acgctggcga cctcggggtg gcagtaggtg acgcgcggga
     2941 tgccggcgta gtcgatgggg gtgaccgcca tgccggcggc gtgctcggcg accaggaagc
     3001 cctcctggaa gctcgcgtgc gccaggccca gcgacggtgg cggcaggatg tcgccgaccg
     3061 cgtagaccgc cccgccgtcc caggaggggc cggggcgcag gtaggcgtcc acggggacga
     3121 agccgcggtc gacggtgacg ccggcctcct cgaagcccat gtcctcggtc accgggcggc
     3181 ggccgacggc gatcatgagc acgtcgccct cgagctggct ctcgccgccc ttggcgtcct
     3241 gcacggtcac ccgcacgccg tcgccggcct tctccacctt ggtgaccttg gtgttgacca
     3301 gcgcgtcgat gccggccttc ttgaactcct tggccagggc cttctggctg tcggcgtcct
     3361 cgttcgggat caggccgggc agcatctcca ccacggtgac cttctcggcg ccgtaggcgt
     3421 tccagaccgt ggcgaactcc acgccgaccg cgcccgcgcc caggatgatc gggcgcgtgg
     3481 gcacgcggtc caggaacagc gcgtggtcgg aggtgatgac cgtctcgccg tcgacctcgg
     3541 cgccctccac ggggatctcg cgcggcacgg acccggtggc cagcaccagg cccttggcgg
     3601 cctgcagggt gcgctcgccc tcgtcggtct gcaccacgag cgtgcggggg tcgctgacgc
     3661 ggccggtgcc cgcgacggtc tcgatgccgc gggccttcat ggtggcctgc aggcccttcc
     3721 agttggtgtc gacgatggtg tccttgtact gctgcaccgc cggcacgtcg atgccttcga
     3781 aggtcgcctt cacgccgtac tcggcggcgt gggcggcgtg ctcggccacc tccgcggcct
     3841 gcaggagcgc cttggtgggg atgcaccccc agtgcaggca ggtgccgccg accttggcct
     3901 tctcgaccag cgccaccttc atcccgatgc cggccgcgcg cagggccgtc gagtagccgc
     3961 cggtgccgcc gccgaggacg aggaggtcga aggtgtcggg catcgcgtgg tgctcctgtt
     4021 cgtggtgggt gactctcctg aagaatccta gccctaccgg ttagcctgcc cgctcacacc
     4081 gatggccggg cgccgcccgg cgcgaggagg gcgaccgatg ggcgagcgca ccggcccgct
     4141 gcacgagcgg cacgtcgcgc tgggcgccgc catgaccgac ttcggcggct ggctgatgcc
     4201 cctgcactac ggctccgtgc tggccgagca ccgcgcggtg cgcgaggact gcggcgtgtt
     4261 cgacgtgagc cacctcggcc ggctgctggt gcgcggcgac ggcgcggacg ccgccgtctc
     4321 ggcggtgctc accaacgacg tggcggccct ggcgcccggg cgcgcgcagt actcgctgtg
     4381 cctggacgag gcgggcggca tcgtcgacga cgtgctggtc taccggctca ccggcggctg
     4441 gtccgacggc ctgctggtgg tgcccaacgc cgccaacgcc gcagcggtcg cggagcgcct
     4501 gcgccacgcc gccccggcgg gcacccaggt gcgcgacgtg caggacgagc tggcctgcct
     4561 ggccgtgcag ggcccccgct cggccgaggt ggtcgaggcc atcgggatcc cggtggcccg
     4621 ctacggctac ctcgactgct tcgacatggc cggtgcgggc gtggtggccc gctccggcta
     4681 cacgggggag cgcggctacg agctcttctg cctggccgag cacgcgcccg tggtctggga
     4741 ccggctgctg gccgccggcg cggcgcccgc cgggctgggc gcgcgggaca cgctgcggct
     4801 ggagatgggc tacccgctgc acggcagcga catcggcccg ggcacctcgc cggtggaggc
     4861 gggcctgacg tgggcggtca agggcgagca cgacttcacc gggcgcgccg cgtacgaggc
     4921 ggccaaggca gccgggccgc gccgccggct gcgcggcgtg cgcgtggagg gccgcggcat
     4981 cccgcgcgcg cactgcgcgg tgtggcgcgg cggcgagcgg gtgggagagc tgaccagcgg
     5041 gacgttctcg ccgacgctgg gcgtcggcat cgggatgggg tatctggcgg cgggggtgga
     5101 cgtgggcgcg cgcgtggaga tcgaggtgcg cggcaagcgg ctgcccgccg cggtggtggc
     5161 gccgccgttc gtggaccgca acccgcgcac ctagccgcta ccagacggag acttcgacca
     5221 ggcgggtgat cgtgcccatc gtcgtgcgca cccggacgta gcgcgcgtcg ccctgcaccg
     5281 agaccgccgt cgccggcccg tgcgactgcg tgcgcaccgg cggcgtccag cgcacgccgt
     5341 cggcggacag ttccacgtcc atttccgacg gctcgccgcg cagcaccacc agcgagcgcg
     5401 gcgccgcgcc gccgaggtcg agcacgaccg actcctcgcg cccgccctcg ccgggcgccg
     5461 gcggcggtgg cggcggctct ccgccgcgcg gcatggacgg gtgcagcggc gtgcccaggt
     5521 cgccatcgg
//
LOCUS       DATMKC010000064         8446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_255965, whole genome shotgun sequence.
ACCESSION   DATMKC010000064 DATMKC010000000
VERSION     DATMKC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255965"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VNU01_01840"
     CDS             <1..379
                     /locus_tag="VNU01_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31392.1"
                     /translation="SQIFSMLMEGSEPPPPGFQPAPLWGSPAHLAGLFPEAELSTSVE
                     RLPFRYASVDDWLEFNERNSGPVKLAKQRLEAEGRWDDLRARVRELALAANRASDGGF
                     LLIGEYLLAEVNKPSNPGVRNIP"
     gene            473..1288
                     /locus_tag="VNU01_01845"
     CDS             473..1288
                     /locus_tag="VNU01_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31393.1"
                     /translation="MTLTLDQYKNIQRYLWSTGDLAALDAHVEDAAADLVHRLALTPG
                     ARVLDIATGTGNAALCAAKAGAEVHGIDIAPELFDDAQARAEELGLDITWREGDAEAL
                     PYRDAAFDRVVSNFGVMFAPRHRHAARELVRVLRPGGRFALSTWIPTGIVAMVLHALD
                     SHSPPMPDWAMPAMKWGDPAHVADVFEGLDVELEFATGAVPWVFSSPEDCVRYLEEVA
                     GPVIVAKAALQQLGRWDEARAQMLAGLEPMVRADVGGCWTNAQYLVTVGRRLR"
     gene            1335..1535
                     /locus_tag="VNU01_01850"
     CDS             1335..1535
                     /locus_tag="VNU01_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31394.1"
                     /translation="MMSALLAGAIAVPILDLGSRRGFLPAFAAGSGAASADQLYAGFL
                     GLTLLNPMTVTYFGALVPRAAG"
     gene            complement(1588..2112)
                     /locus_tag="VNU01_01855"
     CDS             complement(1588..2112)
                     /locus_tag="VNU01_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31395.1"
                     /translation="MSEPIRPAAAPEPAPVAVFDIDGVLADVRHRLCHVRAKPKDWQA
                     FFAAAVRDQPHPQGLALLREMARDHDVLLLTGRPEHTRPQTQAWLESHGVGSYPLHMR
                     PAGDRRPAAVVKRQFLRGLVRTRAVALVVDDDPVVVETLRAAGFDVHHADWERRAEQE
                     QAALLEAQEVDGRS"
     gene            complement(2145..4529)
                     /locus_tag="VNU01_01860"
     CDS             complement(2145..4529)
                     /locus_tag="VNU01_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWH31396.1"
                     /translation="MTRLGTRLALLALVLPLVVPLAAKAQPPEFSARITWTAHGIPHV
                     TADDYAGLGYGYGYAFAKDNLCALAEEIVTVNAQRSRWFGPDGEYRSWGNGLRTTNLK
                     SDFFFQHLNDRDATAGYDQPPPLGMSADVKRVVAGYAAGYNRWLADHGTAGTPCDGAP
                     WVRPITELDLKRRFYQLTLLASSFFFAGAIVDAKPPAAGASGLPAAAPPVPAAHEFDP
                     AALPSSESLGIGSNAYGIGKAATANGKGMVLGNPHFPWQGPERFHQMHLTIPGELDVS
                     GAALFGAPGVLIGHNEHVGWSHTVSTAYRFTPYELTLVPGEPTKYVHDGQVRELQPQT
                     VTVQSRRPDGTLEQRSTTIWHSHYGPVVEYPAAFLQWSPAKAYAIRDANAMNYRVLDQ
                     FLAMNKAGSVRELKAAQETWQAIPWVNTMAADSSGEAYYADQSVVPNVPDELVAQCMT
                     PVGAVTFAAARLPVLNGSTSACDWKTDPDAVQPGIFGPANLPDLLRDDYVHNSNDSYW
                     LANPHEPLEGYDRIIGDERSARSLRTRMGLHIMEDRLAGRDGLPGDRWQLPQLGERLF
                     SNRNMSGELAKDAAVTMCSTTPTMISSGGAQVDVAAACQALAGWDTTANLDATGAAVW
                     REFWRRAAAVNGGPWVVPFNPQDPLNTPNTLNVAHPQVRTAFADAVLSLRQFGIDPAA
                     KLGDIQGEYRGAERIPVHGGPHVEGVFNYIAASLGQGGWNDVYHGSSFIMTVSFTDAG
                     PSARTLVTYSQSTDPASPFWADQLRMFSAKQWNEVPFRHSDVQAQAIRRDTVRE"
     gene            complement(4610..5104)
                     /locus_tag="VNU01_01865"
     CDS             complement(4610..5104)
                     /locus_tag="VNU01_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH31397.1"
                     /translation="MSGDLTIREARDDELDIVASLIVDAYAEYAARMSPDAWSFFAQD
                     IANVRGRLGDARLLVAERDGRLIGSVTLYTDWRGAQSGTYSMRLLAVPPECRGQGVGR
                     ALMEDGIARARAAGVSRLVLTAVQEMESVRDLSEHLGFVRDRSLDHEPAPGVRAEGYA
                     LHLG"
     gene            5258..6157
                     /locus_tag="VNU01_01870"
     CDS             5258..6157
                     /locus_tag="VNU01_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWH31398.1"
                     /translation="MDEARPEGHPPPERPGGLMVVGQELPPPSEVNDLLAGAFAGLAL
                     LGAAVFGVGLALRLPMEVYGGGLAVCLLALAIAVRRYFAAAYPDIEAVEPRELPPQES
                     DVARDSRLSSVERAQPPTLPRRLLLAAAGVLGVSFAVPVSSLGPAPGDALRRTAWARG
                     TRLVTTEGRPLKPEDVVTGGIATVWPQGAIQAEDASVVLVRLSGVQPMRPTNLDWVVN
                     GELVAYSKLCTHAGCPVGLFRERDNALFCPCHQSTFDAARGAVPTFGPAARALPQLPL
                     GVDDEGYLVALGDFTEQVGPAFG"
     gene            6154..6969
                     /locus_tag="VNU01_01875"
     CDS             6154..6969
                     /locus_tag="VNU01_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWH31399.1"
                     /translation="MSGRAGAGTRRLRPLLSWALLAVAGTALTAGAPGAQAQQDAIDP
                     ATAIQGADVYTSQCARCHGAAGQGGQTPSGPDAPALRGAHIALVDLVLRTGRMPPGDP
                     DGDTKARRPLTDERRDALLAWMDDQLGLRGEIPRVPVGGNPARGQEVYAANCAQCHGS
                     TGGGGVAGGGAFTPVLVDREPLVIAEAIRMGPFQMPQFGEDVISDRELADTIAFLEEV
                     QGEPGTPVGLVELNPVFAGAFVFAISLAAIVSAMWLSGRVSMFPDAPEDGEQP"
     gene            6966..8294
                     /locus_tag="VNU01_01880"
     CDS             6966..8294
                     /locus_tag="VNU01_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWH31400.1"
                     /translation="MTPWFEWLLQAMDERLRLRAPGRKTLNKVFPDNWSFLLGEIALV
                     SFGILVLTGIFLTMFFSPSTAPVVYDGGAELYRGRELPAAFESIVRLTHDVPGGLFFR
                     RIHRGASHLFIAATVMHLLRVILTGAFRRPREINYHVGIVLLTLAFGAGFTGYSLPFD
                     SLAGTGIRVAYSELLSFPFVGDRLAFWIFGGDFPTGNMIPRFFVFHVLLIPGLLIGLI
                     SLHLAIMARQRHTQFPRPGIDGHRFVLGKPLWPSQFAITTSLVLWTGGLLALAAVLIP
                     WSDVFLIGPYRPGEVGNNAQPDWFLFWLEGALRILPPVEFDLPGVTVSGPFLAGIVLP
                     GLVFGYLAAYPFIERKVYGLHGEWHVLTNPLEVPLRAASAVTVFTFVLILSAAATNDI
                     LSRMTRIPIETITWFFRVTSIVVPAALGYLAWRYAARRGARAGGVASSET"
BASE COUNT         1052 a         3025 c         3110 g         1259 t
ORIGIN      
        1 cagccagatc ttctccatgc tcatggaggg atccgagccg ccgccgcccg gcttccagcc
       61 ggcgcccctg tggggcagtc ccgctcacct cgccggcctg ttcccggagg ctgagctgtc
      121 caccagcgtc gagcggctcc cgttccgcta cgcctccgtg gacgactggc tggagttcaa
      181 cgagcgcaac tccggcccgg tgaagctggc caagcagcgg ctcgaggcgg agggccgctg
      241 ggacgacctg cgcgcccgcg tccgcgagct ggcgctggcg gcgaaccgtg cctcggacgg
      301 cggcttcctg ctgatcgggg agtacctgct cgccgaggtg aacaaaccgt cgaatccggg
      361 tgtccgaaat atcccgtagt caccgggtgc tgatgcgcgt acggtgactc acggggatgg
      421 gctggcatgg aggtggggca atgccgatcc cggcgtggtg gagcgaccac agatgacgct
      481 cacccttgac cagtacaaga acatccagcg gtacttgtgg tccacgggcg acctggccgc
      541 cctcgacgcg cacgtcgagg acgcggccgc cgacctggtg caccgcctgg cgctgacacc
      601 cggcgcgcga gtcctggaca tcgcgacggg caccggcaac gcggcgctgt gcgccgccaa
      661 ggccggcgcg gaggtgcacg gcatcgacat cgcgccggag ctgttcgacg acgcgcaggc
      721 gcgcgccgag gagctcggcc tggacatcac ctggcgtgag ggggacgccg aggcgctgcc
      781 gtaccgggac gcagcgttcg accgggtggt gtccaacttc ggtgtgatgt tcgcgccgcg
      841 ccaccggcac gccgcccgcg agctcgtgcg cgtgctccgg cccggggggc gcttcgcgct
      901 cagcacctgg atccccacgg gcatcgtggc catggtcctg cacgcgctgg actcgcactc
      961 cccgcccatg ccggactggg cgatgcccgc catgaagtgg ggcgaccccg cgcacgtggc
     1021 cgacgtcttc gaaggcttgg acgtggagct ggagtttgcc accggcgccg tcccgtgggt
     1081 gttctcctcc cccgaggact gcgtgcgcta cctggaggag gtcgccggcc ccgtcatcgt
     1141 cgcaaaggcg gcgctgcagc agctcgggcg ctgggacgag gcccgcgcgc agatgctggc
     1201 cggcctggag ccgatggtgc gggcggacgt gggcgggtgc tggacgaacg cgcagtacct
     1261 ggtgaccgtg ggccgcaggc tgcgctgagg cggcccgcgt atcctgcggc gtccccgacg
     1321 caggagcgcc cgcgatgatg tccgcgctgc tggccggcgc gatcgccgtg ccgatacttg
     1381 acctggggtc gcggcggggc ttcctgccgg ccttcgccgc cggatcgggc gccgccagcg
     1441 cggaccagct ctacgcgggc ttcctgggcc tgacgctgct caaccccatg acggtcacct
     1501 acttcggcgc gctggtgccg cgcgccgcag gctgaccggg gccgccggca gcctcgtgat
     1561 cctcgcgctg gcggcccgca tcctggtcta ggagcggccg tcgacctcct gggcctccag
     1621 cagggcggcc tgctcctgct ccgcgcggcg ctcccagtcg gcgtggtgca cgtcgaagcc
     1681 ggccgcgcgc agggtctcga ccaccacggg gtcgtcgtcc accaccagcg cgaccgcgcg
     1741 cgtgcgcacc aacccccgca ggaactgccg cttcaccacc gctgccgggc ggcggtcccc
     1801 cgccggccgc atgtgcagcg ggtagctgcc cacgccatgc gactcaagcc aggcctgtgt
     1861 ctgcggacgc gtgtgctccg gccggcccgt gagcagcagc acgtcgtggt cgcgcgccat
     1921 ctcgcgcagc agcgccaggc cctgcgggtg cggctggtcc cggaccgccg cggcgaagaa
     1981 cgcctgccag tccttcggct tggcgcgcac gtggcacaga cggtgccgca cgtcggccag
     2041 cacgccgtcg atgtcgaaga ccgccaccgg ggcgggctcc ggcgcggccg cggggcggat
     2101 cggctcgctc atgacgaggc gatcctgacc cccgcggcca ccgcctactc cctgaccgtg
     2161 tcccgccgga tggcctgcgc ctgcacgtcc gaatggcgga acggcacttc gttccactgt
     2221 ttcgcggaga acatccgcag ctggtccgcc cagaacggcg acgccgggtc ggtggactgc
     2281 gagtaggtca ccagcgtgcg cgcggagggc ccggcgtcgg tgaacgacac cgtcatgatg
     2341 aagctggagc cgtggtacac gtcgttccag ccgccctggc cgagggacgc ggcaatgtag
     2401 ttgaacacgc cctcgacgtg cgggccgccg tgcaccggga tgcgctcggc gccgcggtac
     2461 tcgccctgga tgtctcccag cttggcggcc gggtcgatgc cgaactgccg cagcgacagc
     2521 acggcatccg cgaaggcggt gcgcacctgc gggtgggcga cgttcagggt gttgggcgtg
     2581 ttcagcgggt cctgggggtt gaacggcacg acccaggggc ccccgttcac cgcggcggcc
     2641 cggcgccaga actcgcgcca caccgccgcg ccggtcgcgt ccaggttggc ggtcgtgtcc
     2701 cagccggcga gcgcctggca cgcggccgcg acgtccacct gggcgccgcc cgaggagatc
     2761 atggtcggcg tggtggagca catcgtcacg gcggcgtcct tggccagctc accggacatg
     2821 ttgcgattgc tgaacagccg ctcgcccagc tgcggaagct gccagcggtc gcccggcagg
     2881 ccgtcccggc cggccaggcg gtcctccatg atgtgcaggc ccatccgcgt gcgcaggctc
     2941 cgggccgagc gctcgtcgcc gatgatgcgg tcgtagccct ccaggggctc gtgcgggttg
     3001 gccagccagt agctgtcgtt ggagttgtgc acgtagtcgt cgcgcaacag gtccggcagg
     3061 ttggccggcc cgaagatgcc cggctgcacg gcgtcggggt cggtcttcca gtcgcaggcg
     3121 ctggtcgagc cgttcagcac cggcagccgc gccgccgcga acgtgaccgc gcccacgggc
     3181 gtcatgcact gcgccaccag ctcgtccggc acgttgggca ccaccgactg gtcggcgtag
     3241 tacgcctcgc cgctggagtc ggcagccatg gtgttcaccc aggggatggc ctgccacgtc
     3301 tcctgcgccg ccttgagctc gcgcaccgag ccggccttgt tcatggccag gaactggtcg
     3361 agcacccggt agttcatcgc gttggcgtcg cggatcgcgt aggccttggc cggactccac
     3421 tgcaagaacg cggccgggta ctccaccacg gggccgtagt ggctgtgcca gatggtggtg
     3481 ctgcgctgct ccagggtccc gtcgggccgc cgcgactgca ccgtcacggt ctggggctgc
     3541 agctcccgga cctgcccgtc gtgcacgtac ttcgtgggtt ccccggggac cagcgtgagc
     3601 tcgtagggcg tgaagcggta cgcggtggag acggtgtggc tccagccgac gtgttcgttg
     3661 tgaccgatca gcacgcccgg cgcgccgaac agcgccgcgc cggagacgtc cagctctccg
     3721 gggatggtca gatgcatctg gtggaagcgc tccgggccct gccacgggaa gtgcgggttg
     3781 cccagcacca tgcccttgcc gttggccgtg gcggccttgc cgatgccgta ggcgttgctg
     3841 ccgatgccca gcgactcgct tgacggcagg gcggccgggt cgaactcatg ggctgcgggc
     3901 actggcggtg cggcggctgg cagcccggac gcccccgcgg ccgggggctt ggcgtccacg
     3961 atggcgccgg cgaagaagaa cgagctcgcc agcagcgtga gctggtagaa gcggcgcttc
     4021 aggtccagtt cggtgatcgg gcgcacccac ggcgcaccgt cacacggcgt gccggcggtg
     4081 ccgtggtcgg cgagccagcg gttgtagccg gcggcgtagc cggccacgac gcgcttgacg
     4141 tcggccgaca tgcccagcgg cggcggctgg tcgtagccgg cggtggcgtc gcggtcgttg
     4201 aggtgctgga agaagaagtc gctcttcagg ttggtggtgc gcaggccgtt gccccaggag
     4261 cggtactccc cgtccggccc gaaccagcgc gagcgctgcg cgttgaccgt gacgatctcc
     4321 tccgccagcg cgcagaggtt gtccttggcg aaggcgtagc cgtagccgta gcccaggccc
     4381 gcgtagtcgt ccgccgtcac gtgcgggatg ccgtgggcgg tccaggtgat gcgcgcggag
     4441 aactccggcg gctgcgcctt cgcggccagc ggcaccacga gcggaaggac gagcgcgagc
     4501 agcgccaagc gcgtgccgag acgggtcatg cggatctcct gcgtgcgaac gacatggaca
     4561 cccggaacag ttcgccgccg tcacgcccga ctcctgcccg gccgcgccgc tagccgaggt
     4621 gaagggcgta gccctccgcc cgcacgccgg gggccggctc gtggtccagc gagcggtcgc
     4681 gcacgaagcc gaggtgctcg gacaggtccc gcacggactc catctcctgc accgcggtga
     4741 gcaccagccg cgacaccccg gcggcgcgcg cgcgggcgat gccgtcctcc atgagcgccc
     4801 tgccgacgcc ctggccccgg cactccggcg gcacggccag cagccgcatg ctgtacgtcc
     4861 cggactgggc gccacgccag tcggtgtaga gcgtgacgga cccgatgagg cggccgtcgc
     4921 gctccgccac cagcaagcgg gcgtcgccca ggcggccgcg cacgttggcg atgtcctggg
     4981 cgaagaacga ccaggcgtcg ggcgacatcc gcgcggcgta ctccgcgtac gcgtccacga
     5041 tcagggacgc gacgatgtcc agctcgtcgt cgcgcgcctc gcgaatggtc aggtcgccgc
     5101 tcatggcgtc accctaccca acggccggac agggtgcccg ggcgtagcag ccgattggcc
     5161 gtcggcgcgc ccggcgtgct ctgattgacg cgcgcaccgg cgtggtaggt agtgcggcag
     5221 gtgttcgact cctaccgcga cggacgatgg gagcgacgtg gacgaagcgc gcccggaggg
     5281 ccacccgccc cccgaacggc ctggcggcct catggtcgtc gggcaggagc tgcccccgcc
     5341 cagcgaggtc aacgacctgc tcgccggggc cttcgccggg ctggccctgc tgggtgcggc
     5401 ggtgttcggc gtcggcttgg cgctgcgcct gccgatggag gtctacggcg gcggcctggc
     5461 ggtgtgcctg ctggcgctgg ccatcgcggt gcggcgctac ttcgccgcgg cctacccgga
     5521 catcgaagcc gtggagccgc gggagctgcc gccccaggag tccgacgtgg cccgggattc
     5581 caggctgtcg tccgtggagc gcgcgcagcc gcccacgctg ccgcggcgcc tgctgctggc
     5641 cgccgccgga gtgctgggcg tgtccttcgc cgtgccggtg tcgtcgctgg ggcccgcgcc
     5701 aggcgacgcg ctgcggcgca cggcgtgggc ccggggcacg cggctggtca ccacggaggg
     5761 acgcccgctc aagccggagg acgtggtgac cgggggcatc gccaccgtct ggccgcaggg
     5821 ggccatccag gccgaggacg ccagcgtcgt gctggtacgc ctgtccggcg tgcagccgat
     5881 gcggccgacg aacctggact gggtggtcaa cggcgagctg gtcgcctact ccaagttgtg
     5941 cacccacgcg ggctgcccgg tggggctgtt ccgtgagcgc gacaacgcgc tgttctgccc
     6001 gtgccaccag tccaccttcg acgccgcgcg cggcgccgtg cccacgttcg gcccggccgc
     6061 gcgggcgctg ccgcagctgc cgctcggcgt ggacgacgag ggctacctgg tcgcgctggg
     6121 cgacttcacc gagcaagtcg gaccggcgtt cggatgagcg gccgcgcggg cgcgggcacc
     6181 cggcggctca ggcccctgct gtcctgggcg ctgctggccg tcgcgggcac ggcgctcacg
     6241 gccggggcgc cgggagccca ggcgcagcag gacgcgatcg atcccgccac ggcgatccaa
     6301 ggggccgacg tctacacctc gcagtgcgcg cggtgccacg gcgcggcggg gcagggcggg
     6361 cagacgccga gcgggccgga cgccccggcg ctgcgcggcg cgcacatcgc gctggtcgac
     6421 ctggtgctgc gcaccggccg catgcccccg ggcgaccccg acggcgacac caaggcgcgg
     6481 cggcccctca ccgacgagcg ccgcgacgcg ctgctggcgt ggatggacga ccagctgggc
     6541 ctgcggggcg agatcccgcg ggtgccggtg ggcggcaacc cggcgcgcgg gcaggaggtc
     6601 tatgccgcca actgcgcgca gtgccacggc tccaccggcg gcggcggcgt cgccggcggc
     6661 ggcgcgttca cgccggtgct ggtggaccgc gagccgctcg tgatcgccga ggcgatccgc
     6721 atggggccgt tccagatgcc gcagttcggc gaggacgtga tcagcgaccg ggagctggcg
     6781 gacaccatcg cgttcctgga ggaggtgcag ggcgagcccg gcacgccggt gggcctggtg
     6841 gagctcaacc cggtgttcgc gggcgcgttc gtgttcgcca tctcgctggc ggcgatcgtc
     6901 tcggcgatgt ggctgtccgg gcgcgtgtcg atgttccccg acgccccgga ggacggtgag
     6961 cagccgtgac cccgtggttc gagtggctgc tgcaggcgat ggacgagcgc ctgcgcctgc
     7021 gggcgccggg ccgcaagacg ctgaacaagg tgttcccgga caactggtcg ttcctgctgg
     7081 gcgagatcgc gctggtgtcg ttcgggatcc tggtgctgac cgggatcttc ctcaccatgt
     7141 tcttctcgcc gtccaccgcg ccggtcgtct acgacggcgg cgccgagctg taccgggggc
     7201 gggagctgcc cgcagcgttc gagtcgatcg tgcggctgac ccacgacgtg ccgggcgggc
     7261 tgttcttccg gcgcatccac cgcggcgcgt cgcacctgtt catcgccgcg accgtcatgc
     7321 acctgctgcg cgtgatcctg acgggcgcgt tccgccggcc tcgcgagatc aactaccacg
     7381 tcggcatcgt gctgctcacg ctggccttcg gcgccgggtt caccggctac tcgctgccgt
     7441 tcgactcgct ggccggcacg ggcatccgcg tcgcctacag cgaattgctc agcttcccgt
     7501 tcgtggggga ccgcctggcc ttctggatct tcggcggcga cttccccacc gggaacatga
     7561 tcccgcgctt cttcgtgttc cacgtgctgc tgatcccggg cctgctgatc gggctgatct
     7621 ccctgcacct ggcgatcatg gcgcggcagc gccacacgca gttcccgcgc cccggcatcg
     7681 acgggcaccg cttcgtgctg ggcaagccgc tgtggcccag ccagttcgcg atcaccacgt
     7741 cgctggtgct gtggaccggc ggcctgctgg cgctggccgc ggtgctgatc ccctggagcg
     7801 acgtgttcct gatcggcccc taccggcccg gcgaggtggg caacaacgcc cagcccgact
     7861 ggttcctgtt ctggctggag ggcgcgctgc gcatcctgcc gcccgtggag ttcgacctgc
     7921 ccggcgtgac cgtgtccggg ccgttcctgg ccggcatcgt cctgcccggc ctggtgttcg
     7981 gctacctggc cgcctacccg ttcatcgagc ggaaggtcta cggcctgcat ggcgagtggc
     8041 acgtgctcac caacccgctg gaggtgccgc tgcgggccgc gtccgcggtc acggtgttca
     8101 cgttcgtcct gatcctgtcg gcagcggcga ccaacgacat cctcagccgc atgacgcgca
     8161 tcccgatcga gacgatcacc tggttcttcc gcgtcacgtc gatcgtggtg cccgcggcct
     8221 tgggctacct ggcgtggcgc tacgcggcac ggcgcggcgc gcgtgcgggc ggcgtagcct
     8281 cttcggagac atgagcaccg acaaccgcaa tctcgccctt ctcgcccacc tgtccgccct
     8341 tgtggtcttc atcggcgtgc cctcgttcgt ggggccgctg gtggtctggc tgtggcagcg
     8401 cgacagcgac agcttcgtcg ccgagcacgc ccgggaggcg ctgaac
//
LOCUS       DATMKC010000065         7305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_111359, whole genome shotgun sequence.
ACCESSION   DATMKC010000065 DATMKC010000000
VERSION     DATMKC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111359"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VNU01_01885"
     CDS             complement(<1..498)
                     /locus_tag="VNU01_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWH31401.1"
                     /translation="MSDFLSELSRRVLVFDGGMGTQIQARDLTAEDFAGLEGCNEVLV
                     RTRPDVIRDVHLAYLDAGVDAVETDTFGGAPWVLDEYGLGAETESLNEAAARVAREAV
                     TQHGSGFVIGSIGPGTKAPTLSLGKDPNAADFIDYDRMVDGYVRQVRGLVAGGADALI
                     VETAFD"
     gene            619..1824
                     /locus_tag="VNU01_01890"
     CDS             619..1824
                     /locus_tag="VNU01_01890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWH31402.1"
                     /translation="MEEQSDAARDGGRVLRFPVREATRPVAQRDLPPIGGSALLVGDA
                     QDALAGLLAEHAGSVRAALLDPPYNTASRFHHYDDSLSHDAWLAERREHFALVRELLR
                     EDGSLWVHLDDSEMHYGKVMLDTLFGRSNFVATVVWQKTRSRENRTDLSTTHEYVLVF
                     AKDRRAWAAARNLLPFGEEQLGRYRNPDGDPRGPWTSGDLTAKAGPGRRASQFYDVVT
                     PSGRVVRPAKGMAWRYTRERFDELLADGRITFGDGAQMPRLKRFLSETKGGLVPTTWW
                     PGLEVGTTDTAKKHLRRLFPDLVPFETPKPEELALRVLRIATDPGDLVLDCYAGSGTT
                     PAVAHKSGRRWIAVERERRTVDEFLLPRLDRVVAGEDPWGVTAETGWTGGGDFALLDL
                     AEVSGELAR"
     gene            complement(1802..3004)
                     /locus_tag="VNU01_01895"
     CDS             complement(1802..3004)
                     /locus_tag="VNU01_01895"
                     /inference="COORDINATES: protein motif:HMM:NF021622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31403.1"
                     /translation="MHAQAERWRQLYGLPASPLELTRRGGSWSVRARYLTGFASVAGL
                     HLEILPKFLPEDAREGGTWRRALWRILLLAGDDVLLDEPTEAVRDSDAPLADLLAEAF
                     LRSVSLGAARGLPRGYVERETTEPVLLGRLDESRWAELVTEPWSLPCVVDTLDEDIPI
                     NRLLRWAARHLAADVSSPARARALAEWADTLAGVGVVPPRLEEARRLAHGPQHRALAP
                     AVEIARLLLQSRGEAHGPGQAELQGFLWVTERVFEALVRRVCAQAARRLGLRTVKSSV
                     PLGLPARGQALRTTPDVRLLRGGRTVMVLDAKYKVEGRPTTADAYQVIAGGKVLDCDE
                     VALVWPVQPGGTEDEHEWAVPGAGRPSRLSTLAVDLSRLAEPAGEQALCGRMEEFLRA
                     RLSAPVPR"
     gene            complement(2899..4824)
                     /locus_tag="VNU01_01900"
     CDS             complement(2899..4824)
                     /locus_tag="VNU01_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31404.1"
                     /translation="MALLPVRAVWWRRVLASSFWNIEHSQAYDSGGWGSLFIEVPGRA
                     VDETLAFTGGRRDDEDKPARHTIVARVIGAPSVQAPIDFGRKSGDRMILERQNRFAPS
                     AARHPAWDPARGFPAAPPGSTDEQIKAACPPGLTVYLVQTADGSYWAGFKVDEDYPQD
                     WPDDPRLRAIYEQQDGVRMVDSSGAGEPEAKVISQIFDAWSRGRGALLYGPPGTGKTR
                     ILSTLWQMLAQDDGGPGLLLDTDDAGNPFRPAGLEVPLPTPLRTDWLTFHQNTSYADL
                     MLGLRPQPEDGGTALKPRMGRLLDAIWEVADPDTDTASAVIVIDEINRANAARAFGEM
                     LTFLDAEYRAADAEGKDNPLRVPVPLPQLDVRDGRTQELQRPLGGSAALPHPFHMPHH
                     VYLVASMNSVDRAAIPLDSALARRFDRIDLRPDLALLAGALGLDWTALAQQAEQVRGG
                     DGGDWERLSAEETAVLLLDRLNVAIAADMGEEFELGHGLLWEVVLAEPPDRWTALTKA
                     WDAELRPQLEDRCAGRPQQLARLLKVDDPPEGGDYAFRPRARLGVREEDGPQIAQPVR
                     LAGLPAPHARRARPLTAPAWPCGSSARRSGWATSARTRSSSCTPRQNGGASSTACPRL
                     PWSSRAAAGPGASGPAT"
     gene            complement(4824..5846)
                     /locus_tag="VNU01_01905"
     CDS             complement(4824..5846)
                     /locus_tag="VNU01_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HWH31405.1"
                     /translation="MYRYLGNKTKLAAWIVDVARTVARPPARVADPMCGTASVAWAFA
                     QAGYDVTVADELRFPVLHAKARLLCKRRPAFRGAPGGSYDGVLRALNGLPGQEGLFHR
                     EYSDGGTPRNGCPPRRYFTAHNAARIDAIRARIRDWRAEGALTGREADLLLHDLVLAV
                     NRVANIAGTYGYFRSSWNRSSLEPLTLAPTVFVPTPGRHAVLQGRVEDLAARLDVDLC
                     YLDPPYTKRQYAGQYHIPETIAREDEPEPVGQGGLRDWYPQSSAFCSKRTVAEAFREV
                     LKRVGAAHVLISYSEDGLLAPDAALELFSEFGRVTRHELDIKRFRSNGGKEGSVTEHL
                     YHVELA"
     gene            6029..6610
                     /locus_tag="VNU01_01910"
     CDS             6029..6610
                     /locus_tag="VNU01_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31406.1"
                     /translation="MQSPITEDGLHIAGEDEIDLDNDALDPDALDPDERHAEERTWSD
                     ADADGELESLRAAFVDAFNSRDLEDLLGIVAADVEVPDLPGEDGAGALAMELERMWAN
                     APALLLTRAFLDGRPCAVAWLPDEEDCWSRVALVCFEHDGDKLTLVAVPDDVDAMERA
                     EAEAPDGDELDEWSDWSEWETGEETHLRVRLNA"
     gene            complement(6614..>7305)
                     /locus_tag="VNU01_01915"
     CDS             complement(6614..>7305)
                     /locus_tag="VNU01_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWH31407.1"
                     /translation="AGQEGVWLGAEATERLLSAFGVTTAMARVVHDAEAAVRAAREIG
                     GKVAVKSAAPVHKTELGGVHLGLEAPEDISEAVYAISRELVRAEHGELEEHGFLVQAM
                     AGEGVEMMVGVTHDATFGPILVAGMGGTLVELLRDVSVRIHPLTDTDVRDMLTSLRGY
                     PLLTGYRGAPPADIAALEDLLYRLSALVEEVPELAELDCNPVFVHREHNGVTVVDARV
                     RLAPGHRRPRR"
BASE COUNT          964 a         2704 c         2670 g          967 t
ORIGIN      
        1 gtcgaaggcc gtctccacga tgagcgcgtc ggcgccgccg gccaccagcc cgcggacctg
       61 gcgcacgtag ccgtcgacca tgcggtcgta gtcgatgaag tcggcagcgt tcgggtcctt
      121 gcccagtgac agcgtgggcg ccttggtgcc cgggccgatg gagccgatca cgaagccgga
      181 accatgctgc gtgaccgcct cgcgggccac ccgggcggcg gcctcgttga gcgactcggt
      241 ctccgccccc aggccgtact cgtccagcac ccagggcgcg ccgccgaacg tgtcggtctc
      301 cacggcgtcg acgccggcgt ccaggtaggc caggtgcacg tcgcggatga cgtccggacg
      361 ggtgcgcacc aggacctcgt tgcagccctc caggcccgcg aagtcctctg cggtgaggtc
      421 gcgtgcctgg atctgcgtgc ccatgccacc gtcgaagacc aggacgcggc gggacagttc
      481 ggagaggaag tcgctcatgg tgtgccgcca tggtagtccg ccccccgcct ggcggggaca
      541 cggcggcgtg gcgccaagcg ggagacgaca caccgacgtg attcacttgc cgccgacgcc
      601 gaccacgagg acgcacgcat ggaagagcag tctgacgccg cgcgcgatgg cggccgggtg
      661 ctgcgcttcc cggtgcggga ggccactcgc cccgtcgccc agcgagacct cccgcctatc
      721 ggcggcagcg cgctgctcgt cggcgacgcg caggacgcgc tggccggctt gctcgccgag
      781 cacgccggct ccgtgcgcgc ggcgctgctg gacccgccct acaacaccgc gtcccggttt
      841 catcactacg acgactcgct gtcgcacgac gcgtggctcg cggagcggcg cgagcacttc
      901 gcgctcgtgc gggaactgct gcgcgaggac ggctccctgt gggtccacct ggacgacagc
      961 gagatgcact acggcaaggt catgctcgac acgctgttcg gccggtccaa cttcgtcgcc
     1021 accgtggtgt ggcagaagac gcgcagccgc gaaaaccgca ccgacctgtc gacgacccac
     1081 gagtacgtcc tggtgttcgc caaggaccgg cgcgcctggg cggccgcccg caacctgctg
     1141 ccgttcggcg aggagcagct cgggcgctac cgcaacccgg acggtgaccc gcgcggcccc
     1201 tggacgtctg gcgacctcac ggccaaggcg ggtcccgggc gcagggcgtc gcagttctac
     1261 gacgtggtga cgccgagcgg ccgcgtggtc cgtcccgcca agggcatggc gtggcgctac
     1321 acgcgcgagc ggttcgacga actgctcgcc gacgggcgca tcaccttcgg ggacggcgcg
     1381 cagatgccgc gcctcaagcg gttcctctcc gagaccaagg gcggcttggt gcccaccacg
     1441 tggtggcccg gcttggaggt cggcaccacg gacacggcca agaagcacct gcggcggctg
     1501 ttcccggacc tggtgccgtt cgagacgccg aagccggagg agctggcgct gcgggtgctg
     1561 cgcatcgcca cggatcccgg cgacctggtg ctggactgct acgcggggtc gggcacgacg
     1621 ccggcggtcg cccacaagtc cgggcggcgc tggatcgcgg tggagcgcga gcgccgcacc
     1681 gtcgacgagt tcctgctgcc ccggctggac cgtgtcgtcg cgggtgagga cccctggggc
     1741 gtgaccgccg agaccggctg gacgggcggg ggcgacttcg cgttgctgga cctcgcggag
     1801 gtcagcgggg aactggcgcg ctgagccggg cacggaggaa ttcctccatg cgcccgcaca
     1861 gcgcctgctc gccggccggc tcggcgaggc gtgagaggtc cacggccaag gtggacaggc
     1921 gcgacggccg cccggcgcca ggcaccgccc actcgtgttc gtcctcggtg ccgccgggct
     1981 gcacgggcca gaccagcgcc acctcgtcgc agtcgagcac cttcccgccg gcgatcacct
     2041 gataggcgtc cgccgtcgtc ggcctgccct ccaccttgta cttcgcgtcc aggaccatga
     2101 cggtccggcc gccccgcagc agccgcacgt ccggcgtggt gcgcagggcc tggccgcgcg
     2161 ccggcagtcc cagcggcacc gacgacttca cggtgcgcag gcccaggcgc cgggcggcct
     2221 gcgcgcagac gcgtcgcacc agcgcctcga acacgcgctc ggtcacccag aggaacccct
     2281 gcagctccgc ctggcccggt ccgtgcgcct cgccgcggct ctgcagcagg agcctggcga
     2341 tctccacggc gggggccagc gcgcggtgct gcgggccgtg agccaggcgc cgcgcctcct
     2401 ccaggcgagg cggcaccacg ccgacccccg ccagcgtgtc ggcccattcg gcgagcgcgc
     2461 gcgcccgcgc cggcgacgac acgtccgcgg cgaggtgccg cgccgcccag cgcagcagcc
     2521 ggttgatcgg gatgtcctcg tcgagcgtgt ccacgacgca cggcagcgac cacggctcgg
     2581 tgacgagctc ggcccaccgg ctctcgtcga ggcgtccgag caggacgggc tcggtcgtct
     2641 cccgctcgac gtagccgcgt ggcagccccc gcgccgcccc cagggagacc gagcgcagga
     2701 aggcctccgc gagcaggtcc gccagcgggg cgtcgctgtc tcgcacggcc tcggtcggct
     2761 cgtccagcag cacgtcgtcg cccgcgagca gcaggatccg ccacagcgcg cggcgccagg
     2821 tgccgccctc gcgggcgtcc tccggcagga acttgggcag gatctccagg tgcaggcccg
     2881 cgacggacgc gaaaccggtc aggtagcggg cccggacgct ccaggacccg ccgcggcgcg
     2941 tgagctccag gggagacgcg ggcaggccgt agagctggcg ccaccgttct gcctgggcgt
     3001 gcaactgctc gacctggtgc gcgccgatgt cgcccagccg gaccgcctcg ccgaactccc
     3061 gcagggccag gctggcgcgg tgagcggtcg cgcccgccgc gcgtgggggg cggggagtcc
     3121 ggccagccgc accggctgcg cgatctgcgg cccgtcctcc tcgcgcacgc ccaggcgggc
     3181 gcgaggccgg aacgcgtagt caccgccctc cggcgggtcg tccaccttga gcaggcgcgc
     3241 cagctgctgt ggccggccgg cgcagcggtc ctccagctgc gggcgcaact cggcgtccca
     3301 cgccttggtc agcgcggtcc agcgatccgg cggctcggcc agcacgacct cccacagcag
     3361 gccatggccg agctcgaact cctcgcccat gtccgcggcg atggcgacgt tcaggcggtc
     3421 cagcagcagt accgcggtct cctccgcgct gagccgctcc cagtcgccgc cgtcgccgcc
     3481 gcgcacttgt tcggcctgct gcgccagcgc ggtccagtcc aggccgagcg ctccggccag
     3541 cagtgccagg tccgggcgca ggtcgatccg gtcgaaccgg cgcgccagtg cgctgtccag
     3601 cgggatcgcg gcccggtcga cggagttcat ggacgccacc aggtagacgt ggtggggcat
     3661 gtggaacggg tgcggcaggg ccgcggagcc acccaggggc cgctgcagct cctgcgtgcg
     3721 cccgtcgcgg acgtccagtt gcggcagcgg cacggggacg cgcagcgggt tgtccttgcc
     3781 ctccgcgtcc gccgcccggt actccgcgtc caggaacgtc agcatctcgc cgaaggcgcg
     3841 cgcggcgttg gccctgttga tctcgtcgat gacgatcacc gccgaggccg tgtccgtgtc
     3901 ggggtcggcg acctcccaga tggcgtcgag gagccggccc atgcgcggct tgagggccgt
     3961 gcccccgtcc tcgggctggg gacgcaggcc cagcatcagg tccgcgtagg aggtgttctg
     4021 gtggaaggtg agccagtccg tgcgcagcgg cgtgggcagg ggcacctcca ggccggccgg
     4081 gcggaacggg ttgccggcgt cgtcggtgtc cagcagcagc cccgggccgc cgtcgtcctg
     4141 ggccagcatc tgccacagcg tgctcaggat gcgcgtcttg ccggtgccgg gcgggccgta
     4201 cagcagcgca ccgcgccccc ggctccacgc gtcgaagatc tgcgagatca ccttcgcctc
     4261 gggctcgcca gcgccggagc tgtccaccat gcgcacaccg tcctgctgct cgtagatcgc
     4321 ccgcaggcgc gggtcgtccg gccagtcctg cgggtagtcc tcgtccacct tgaaccccgc
     4381 ccagtaggac ccgtcggcgg tctgcaccag gtacaccgtg agcccgggcg ggcacgccgc
     4441 cttgatctgc tcgtcggtgc tgccgggcgg cgctgcgggg aacccgcgcg cggggtccca
     4501 tgccgggtgg cgcgcggcgc tgggcgcgaa gcggttctgg cgctccagga tcatgcggtc
     4561 gccggacttg cggccgaagt cgatcggggc ctggacgctc ggcgcgccga tcacccgcgc
     4621 gacgatggtg tgccgcgccg gcttgtcctc gtcgtcgcgc cgcccgccgg tgaaggcgag
     4681 cgtctcgtcg acggcgcggc cggggacctc gatgaacagc gaaccccagc cgccgctgtc
     4741 ataggcctgc gagtgctcga tgttccagaa gctgctcgcc aggacgcgcc gccaccacac
     4801 ggccctcacc ggcagcagcg ccatcaggcc agctccacgt ggtagaggtg ctcggtcacc
     4861 gagccttcct tgcccccgtt gctgcggaag cgcttgatgt ccagctcgtg gcgcgtcacg
     4921 cgcccgaact ctgagaacag ctccagcgcg gcgtccgggg ccagcagccc gtcctcgctg
     4981 tagctgatca gcacgtgggc cgccccgacc cgcttgagca cctcccggaa ggcctcggcc
     5041 acggtgcgct tggagcagaa cgccgaggac tgcgggtacc agtcccggag tccgccctgg
     5101 cccaccggct ccggctcgtc ctcccgcgcg atcgtctcgg ggatgtggta ctgcccggcg
     5161 tactggcgct tggtgtacgg cgggtccagg tagcacaggt ccacgtcgag ccgggcggcc
     5221 aggtcctcca cgcgaccttg cagcaccgcg tgccgccccg gggtgggcac gaacaccgtc
     5281 ggcgccaggg tcagcggctc cagcgacgag cggttccacg acgagcggaa gtagccgtac
     5341 gtgccggcga tgttggccac ccggttgacc gccaggacca ggtcgtgcag cagcaggtcc
     5401 gcctcccggc cggtcagcgc tccttcggcg cgccagtcgc ggatccgcgc gcggatggcg
     5461 tcgatgcggg cggcgttgtg cgcggtgaag tagcgccggg gcgggcagcc gttgcgcggt
     5521 gtcccgccgt cgctgtactc gcggtggaac aggccctcct ggccgggaag gccgttgagg
     5581 gcgcgcagga cgccgtcgta ggagccgccc ggggccccgc ggaaggcggg ccgccgcttg
     5641 cacagcaggc gcgccttggc gtgcaggacc gggaagcgca gctcgtcggc caccgtgacg
     5701 tcgtagccgg cctgggcgaa ggcccaggcg acgctggcgg tcccgcacat cgggtccgcc
     5761 acccgcgccg gcgggcgcgc gaccgtccgg gccacgtcga cgatccacgc ggccagcttc
     5821 gtcttgttgc ccagatagcg gtacaagcgc gctcccggaa cccggtggcc tcaacggtcc
     5881 ggagtgtacg ggggaggggt gacagcctcc ggcgggtgac taccgccaat tgacgaattg
     5941 gggtcttccc aagcggccgg tgccttggga tgctcatgtc gcgaagtccg atcgcacgcc
     6001 gcccgcggcg tcgacgaagg agagggccat gcagagcccg atcaccgagg acggcctgca
     6061 catcgccggc gaggacgaga tcgacctcga caacgacgcg ctggacccgg acgcgctcga
     6121 cccggacgag cgccacgccg aggagcgcac gtggagcgac gccgacgccg acggcgagct
     6181 ggagtccttg cgcgcggcct tcgtggacgc cttcaacagc cgcgacctcg aggacctgct
     6241 cggcatcgtc gccgccgacg tggaggtgcc cgacctgccg ggcgaggacg gcgccggcgc
     6301 gctcgccatg gagctggagc ggatgtgggc caacgccccc gcgctgctgc tcacccgcgc
     6361 cttcttggat ggccgcccct gcgcggtcgc gtggctgccg gacgaggagg actgctggtc
     6421 ccgggtggcg ctggtgtgct tcgagcacga cggcgacaag ctcacgctgg tggcggtgcc
     6481 cgacgacgtc gacgcgatgg agcgcgccga ggcggaggcg ccggacggtg acgagttgga
     6541 cgagtggtcc gactggagcg agtgggagac cggcgaggag acccacctgc gcgtgcgcct
     6601 caacgcctga ccgtcagcgg cgggggcggc ggtggccggg ggccaggcgg accctggcgt
     6661 ccaccacggt cacgccgttg tgctcgcggt gcacgaacac cgggttgcag tccagctccg
     6721 ccagctccgg cacctcctcc accagcgcgg acagccggta cagcaggtcc tccagcgccg
     6781 cgatgtcggc gggtggcgcg ccgcggtagc cggtcagcag cgggtagccg cgcagggacg
     6841 tgagcatgtc gcgcacgtcg gtgtcggtca gcgggtggat gcgcacgctc acgtcgcgca
     6901 gcagttccac cagggtgccg cccattccgg cgaccaggat ggggccgaac gtggcgtcgt
     6961 gggtgacgcc gaccatcatc tcgacgcctt cgccggccat cgcctgcacc aggaagccat
     7021 gctcctccag ctccccgtgc tccgcgcgca ccagctcgcg cgagatcgcg tacaccgcct
     7081 cgctgatgtc ctcgggtgcc tccagcccca ggtgcacgcc gcccagctcc gtcttgtgca
     7141 ccggcgcggc ggacttcacg gcgaccttgc cgccgatctc gcgcgccgcg cgcacggcgg
     7201 cctccgcgtc gtggaccacc cgcgccatgg cggtggtgac gccgaaggcc gacagcagcc
     7261 gctcggtcgc ctcggcgccc agccacaccc cttcctggcc tgccc
//
LOCUS       DATMKC010000066         2917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_41909, whole genome shotgun sequence.
ACCESSION   DATMKC010000066 DATMKC010000000
VERSION     DATMKC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41909"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VNU01_01920"
     CDS             complement(<1..213)
                     /locus_tag="VNU01_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWH31408.1"
                     /translation="MTSPAARRSRGDQTRERLLDAAEELMAERGVHGPSLAEITTRAG
                     QRNNSALSYHFGGREGLLRALAARHQE"
     gene            323..1384
                     /locus_tag="VNU01_01925"
     CDS             323..1384
                     /locus_tag="VNU01_01925"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWH31409.1"
                     /translation="MTISPDRIPERTSTPARFNGLPMPKQASLLFEEDLRPRVIREHR
                     GRWPFPIPNGWFIVAMSEDLPVEGVEPIYAFGKELVLFRDAFGAPRVLDAHCPHLGAH
                     LGVGGQVDGSCLRCPFHGWAFNGESGRCEDVPYDESKKMPTRARIGTYPTIERNGAIW
                     AWHHLEGGAPFYDVPEVFEFSSPDWSAPVMREFTIRTCAQEMAENNVDLAHFKFVHGF
                     AAEPEENFVVDGAYKRAERNDGSFTREGFGLGLGVLRVRDFVTWISSTTPIDRDTVHV
                     RWIFTAPAKNGPEGLENAVHRFCEGISQDIPIWENKIYQDRPVLRGMEGDIIQHRRWS
                     EQFYSGWPVDEVPAQGGPA"
     gene            1381..2199
                     /locus_tag="VNU01_01930"
     CDS             1381..2199
                     /locus_tag="VNU01_01930"
                     /inference="COORDINATES: protein motif:HMM:NF006114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH31410.1"
                     /translation="MSKTWFITGASRGFGRLWSQAALERGDRVAATARDTATLKDLAE
                     AYGDAVLPLQLDVTDRAACRETVQQAHDRFGRLDVLVNNAGYGHFGFAEELTEQEARA
                     QLETNFFGALWTTQAAIPLMRAQGSGHLVHVSSLAGAVAFPGLSMYHASKWALEGMAE
                     SVAGEVAGFGIRTTIIEPGGYSTDWGADSAARSEGIDLYDEVRELIAAGAHPSAQPDP
                     SLTVPAVLAAVDADEPPLRLLLGSFAFDMVVPIVERRVQDWRAWEDLSRSASPS"
     gene            2196..2726
                     /locus_tag="VNU01_01935"
     CDS             2196..2726
                     /locus_tag="VNU01_01935"
                     /inference="COORDINATES: protein motif:HMM:NF039361.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6158 family protein"
                     /protein_id="HWH31411.1"
                     /translation="MTAVPVAELSDEELERQCTVVHATRHWTFLHGTAEQFRTHTERM
                     LVLEQEYLRRFPQRTWQGGGADAAAAGEDDADAQESARLRLLLLSLKAQVDAALVVPP
                     AAGPAPDDDAVRALLRAVGDAGGRLHKLALHQLARSHGLTPPELATLYRSDPPLLRVE
                     RDERVLTDAGRARLSG"
BASE COUNT          393 a         1086 c         1063 g          375 t
ORIGIN      
        1 ctcctggtgc cgggcggcca gcgcccggag cagcccttcg cggccgccga agtggtagct
       61 cagcgcggag ttgttgcgct ggccggcccg ggtggtgatc tcggccaggg acggcccgtg
      121 gaccccgcgc tcggccatca gctcctcggc cgcgtcgagc agccgctcgc gggtctggtc
      181 gccgcgggag cggcgcgccg cggggctggt cacggcgtgg agtgaaccac cggaggggct
      241 tgcgctcgga cgcggcaggg gccacagtaa gaagaccttc tcaaatgcac cacgccgacg
      301 cgtggcggac aggagcctcc ccgtgacgat ctcgcccgat cggatccccg agcgcacgtc
      361 cacgccggcg cgcttcaacg ggctgccgat gcccaagcag gcgtccctgc tgttcgagga
      421 ggacctgcgc ccgcgcgtga tccgggagca ccggggccgg tggcccttcc cgatccccaa
      481 cggctggttc atcgtcgcga tgagcgagga cctgccggtg gagggcgtgg agccgatcta
      541 cgccttcggc aaggagctgg tgctgttccg cgacgccttc ggggcgccgc gcgtgctcga
      601 cgcccactgc ccgcacctcg gtgcccacct cggcgtcggc gggcaggtgg acgggagctg
      661 cctgcgctgc ccgttccacg gctgggcgtt caacggcgag tccggcaggt gcgaggacgt
      721 cccgtacgac gagagcaaga agatgccgac ccgggcgcgc atcgggacct acccgacgat
      781 cgagcgcaac ggcgccatct gggcgtggca ccacctcgag ggcggcgcgc cgttctacga
      841 cgtgccggag gtcttcgagt tctccagccc cgactggtcg gcgccggtca tgcgcgagtt
      901 cacgatccgc acctgcgcgc aggagatggc cgagaacaac gtcgacctcg cgcacttcaa
      961 gttcgtgcac ggcttcgccg ccgagcccga ggagaacttc gtcgtcgacg gcgcgtacaa
     1021 gcgcgcggag cgcaacgacg ggtcgttcac ccgcgagggg ttcggcctgg gcctcggcgt
     1081 cctgcgcgtc cgcgacttcg tcacctggat ctcgagcacg acgcccatcg accgcgacac
     1141 ggtgcacgtc cgctggatct tcaccgcgcc cgcgaagaac ggcccggagg gcctggagaa
     1201 cgccgtgcac cgcttctgcg agggcatcag ccaggacatc ccgatctggg agaacaagat
     1261 ctaccaggac cgcccggtgc tgcgcggcat ggagggcgac atcatccagc accgccggtg
     1321 gagcgagcag ttctacagcg gctggccggt cgacgaggtg ccggcgcagg gcggcccggc
     1381 atgagcaaga cctggttcat caccggtgcg tcccgcggct tcggccgcct gtggtcgcag
     1441 gccgcgctgg agcggggcga ccgcgtcgcg gcgaccgccc gcgacaccgc gacgctgaag
     1501 gacctcgccg aggcgtacgg cgacgcggtc ctgccgctgc agctcgacgt caccgaccgc
     1561 gccgcctgcc gcgagaccgt gcagcaggcg cacgaccgct tcggccggct cgacgtcctg
     1621 gtgaacaacg ccggctacgg ccacttcggc ttcgccgagg agctgaccga gcaggaggcg
     1681 cgggcccagc tcgagaccaa cttcttcggc gcgctctgga cgacgcaggc ggcgatcccg
     1741 ctgatgcgcg cgcagggcag cgggcacctg gtgcacgtgt ccagcctggc cggcgccgtg
     1801 gcgttcccgg gcctgtcgat gtaccacgcc tccaagtggg cgctggaggg catggccgag
     1861 tccgtggccg gcgaggtggc cggcttcggc atccgcacga ccatcatcga gccgggcggc
     1921 tacagcaccg actggggcgc tgactccgcc gcccgctccg agggcatcga cctgtacgac
     1981 gaggtgcgcg agctcatcgc cgccggcgcc cacccgagcg cccagccgga cccgtccctc
     2041 accgtgccgg cggtgctcgc cgccgtggac gcggacgagc cgccgctgcg cctgctgctc
     2101 gggtccttcg ccttcgacat ggtcgtgccg atcgtggaga ggcgcgtcca ggactggcgc
     2161 gcctgggagg acctgagccg atcggccagc ccctcgtgac cgccgtcccg gtcgcggagc
     2221 tgtccgacga ggagctcgag cggcagtgca ccgtcgtgca cgccacccgg cactggacct
     2281 tcctgcacgg caccgccgag cagttccgca cgcacaccga gcggatgctg gtgctggagc
     2341 aggagtacct gcgccgcttc ccgcagcgca cctggcaggg cggcggggcg gacgccgcgg
     2401 ccgccggcga ggacgacgcc gacgcgcagg agtctgcgcg cctgcgcctg ctgctgctca
     2461 gcctcaaggc acaggtcgac gcggcgctcg tcgtgccgcc cgccgcagga ccggcacccg
     2521 acgacgacgc cgtccgggcg ctgctgcggg ccgtcggcga cgccggcggc cggctgcaca
     2581 agctcgccct gcaccagctg gcccgctcgc acggcctgac gccgcccgag ctggccacgc
     2641 tgtaccgcag cgacccgccg ctgctgcgcg tcgagcggga cgagcgcgtg ctgacggacg
     2701 cggggcgcgc gcggctgtcc ggctgagcac cgccgcgggc gcggcgtcag ccctgcgcgc
     2761 tggggaaccg cggcggccgc ttgccgacga aggccgccac cccctcgagc ccgtcgcggc
     2821 cgcctgcgag gtcggcgatg gactgcgcct cggcgtccag ctgctcgtcg taggccgcgc
     2881 cgcccgcctg ctcgaccagc cgccgcgtgg cctgcag
//
LOCUS       DATMKC010000067         2082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_255274, whole genome shotgun sequence.
ACCESSION   DATMKC010000067 DATMKC010000000
VERSION     DATMKC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255274"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VNU01_01940"
     CDS             <1..243
                     /locus_tag="VNU01_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31412.1"
                     /translation="LLDEPLLAALGLPAPSPADRRLVEGTLRARARLLAELPPRRHPK
                     TVTDRPHRTYPAGYAIDELGPEGAGGGLPSPLEAGA"
     gene            240..1004
                     /locus_tag="VNU01_01945"
     CDS             240..1004
                     /locus_tag="VNU01_01945"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWH31413.1"
                     /translation="MTGGLDDHRLAAELARRAGEALLELRAEAERDPGRRWTLRDDGD
                     LLAHRLLMDALRQARPGDAVLSEEGRDHPARLTAERVWIVDPLDGTREYGDPPRDDWA
                     VHVALWSATAGDLVAGAVALPARGVVLSTAEASAPLRQAPPAPRLLVSRSRPHPLVTH
                     VIAPAVDGVLVPMGSAGAKAMAVVLGEAEVYAHAGGQYEWDSAAPVAVARAAGLHASR
                     LDGSPLVYNREDPYLPDLLICRPELADRVLAALAAA"
     gene            complement(1465..>2082)
                     /locus_tag="VNU01_01950"
     CDS             complement(1465..>2082)
                     /locus_tag="VNU01_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31414.1"
                     /translation="RSVSTSPGGRSDVVAGTPRHRARRLLWVVVLWGLAGCGSRVPPP
                     YEVLAADPSLTPSAFEAPIPTASVPSQAAPPSPDSVAAEASPTESFRPFPGSTLYQNG
                     VITITLSATCARHGQVMTADIQAPVRTTVSMFARYSDGDPHGNMFIAPSDDQGRYTWT
                     FVVPPNAPPGFAQVFAASGGPDNSAGGGGIGSADFTLVGHGQPCA"
BASE COUNT          280 a          719 c          810 g          273 t
ORIGIN      
        1 ctgctggacg agccgctgct tgcggcgctt gggctgccgg ccccgtcgcc ggccgatcgc
       61 cgcctggtcg agggcacgct gcgggcgcgg gcgcggctgc tcgccgagct gccgccccgg
      121 cggcacccca agaccgtgac ggacaggccg caccggacct atccggccgg atacgcgatc
      181 gacgaactgg gccccgaggg cgcggggggc gggctgccgt cgccgctgga ggcgggcgcg
      241 tgaccggcgg gctggacgac caccggctgg cggccgagct cgcccgccgc gccggggagg
      301 cgctgctgga gctgcgcgcc gaggccgagc gcgaccccgg gcggcgctgg acgctgcgcg
      361 acgacgggga cctgctggcc caccggctgc tgatggacgc gctgcgccag gcgcgtcccg
      421 gggacgcggt gctgagcgag gaggggcgcg accaccccgc ccggctgacc gccgagcgcg
      481 tgtggatcgt ggaccccttg gacggcacca gggagtacgg ggacccgccc agggacgact
      541 gggccgtgca cgtcgcgctg tggtcggcga ccgcggggga cctggtggcg ggggcggtgg
      601 cgctgcccgc ccgcggcgtg gtgctgtcca ccgccgaggc gtcggcgccg ctgcggcagg
      661 cgcccccggc gccgcgcctc ctggtcagcc ggtcgcggcc gcacccgctg gtgacgcacg
      721 tgatcgcccc ggccgtcgac ggcgtgctgg tgcccatggg ctccgcgggc gccaaggcga
      781 tggccgtggt gctgggcgag gcggaggtgt acgcgcacgc cggcggccag tacgagtggg
      841 acagcgcggc gccggtcgcg gtggcgcgcg ccgcgggcct gcacgcgtcg cggctggacg
      901 ggtcgccgct ggtctacaac cgcgaggacc cgtacctgcc ggacctgctg atctgccgtc
      961 cggagctggc cgaccgcgtg ctggccgccc tcgccgccgc ctgagtcccc gcccaggccc
     1021 ccaagttgga aggtcaggcc gtataccgtt ctagttatgt gccatgcagt cgggcaccac
     1081 agacgttgag catccaccca cggaattttt ggacgcggct ctgtagcgtg agcggctgtg
     1141 accgtcgaca cgtcgtcgtg ggtacgtgag taaccacatc gagttgatgt cgcgcatggc
     1201 cgacgatcac gacgatcacg tttcaacacg tccgaagtcc cctgagtcct cggggtcgac
     1261 cgtgacaagg tgtgccggct catccacgcg ggtgtccctc cggtccgcag gaaggtggcg
     1321 tgttcttcct ccgtccggaa cgtccaaggg gtggcgcgcc aagttcgagc aaggccagcc
     1381 aagagggacg gcgagctgtg ggcgctagat agggccgagc attcggctgc cgccgccgga
     1441 gtggtcgagt cggtaggtcc ggctttaagc acagggttgg ccgtggccga cgagggtgaa
     1501 gtcggccgag ccgatgccgc cgcctcccgc gctgttgtcg ggaccgccgg aggcggcgaa
     1561 cacctgcgcg aacccgggtg gagcgttggg cggaaccacg aatgtccagg tgtagcgccc
     1621 ttggtcatcg ctgggagcga tgaacatgtt cccgtgcggg tcgccgtcgg agtagcgggc
     1681 gaacatcgac accgtcgtgc gcaccggcgc ctggatgtcg gcggtcatca cctggccgtg
     1741 gcgcgcgcac gtcgccgaca gcgtgatcgt gatgaccccg ttctgataca gggtcgaccc
     1801 ggggaacggg cggaaggatt cggtcggcga ggcctccgcg gcgaccgaat ccggcgatgg
     1861 cggcgcggcc tgggacggca cggaggcggt ggggatgggc gcctcgaagg ccgacggcgt
     1921 cagcgacgga tccgccgcga gcacctcata aggcggcggc acgcgcgagc cgcagcctgc
     1981 caggccccac agcaccacga cccacagcag ccgtcgcgca cgatggcgcg gcgtcccggc
     2041 caccacgtca gacctcccgc caggtgacgt ggagaccgac cg
//
LOCUS       DATMKC010000068         6139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_14850, whole genome shotgun sequence.
ACCESSION   DATMKC010000068 DATMKC010000000
VERSION     DATMKC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14850"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1226
                     /locus_tag="VNU01_01955"
     CDS             81..1226
                     /locus_tag="VNU01_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWH31415.1"
                     /translation="MGAGPLDGVRVLELAAIGPAPFGVMLLADLGAEVIRVDRVANVR
                     TQTGPEFGMRGVGRNRRSVGIDVRTPEGRDLVLALAERADVLVEGFRPGVTERLGVGP
                     GDCMGRNPRLVYARMTGWGQDGPLAPTAGHDLNYAAVGGAIHPIGRADSPPPPPLNFL
                     ADFAGGGCYLALGVLAALVERDRSGRGQVVDAAMVDGVVSLTAFFHGLLRMGAWTTER
                     ESNLLDGGTPWYDTYETADGGFMAVGAVEPQFYAELLARLGLDATDWPQHDRERWPAM
                     RKTFTETFRSRTRQEWTAVFDGSDACVAPVLTLAEAPGHPHLVARQAFVDVAGVPEPA
                     PAPRFSRTPGTVRRPAPAFGQDSDEVLAEFGLDAARVADLRARDVVA"
     gene            complement(1229..1660)
                     /locus_tag="VNU01_01960"
     CDS             complement(1229..1660)
                     /locus_tag="VNU01_01960"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH31416.1"
                     /translation="MSDRVQETEELPGDPASILAIVTDFESYPDWQPQMAEAEVLERD
                     AEGRGTLVRYKFKGQMFTANYTLAYAYDDDGVAFHAVEGDILKTLDGRYTLADAGDGR
                     TRVTYELEASPAIKIPAVLRRQGAKRMVEDLFAGLRAKLDG"
     gene            complement(1707..2666)
                     /locus_tag="VNU01_01965"
     CDS             complement(1707..2666)
                     /locus_tag="VNU01_01965"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH31417.1"
                     /translation="MGALDGRVAIITGAGRGIGREHALLFAEEGARVVVNDLGGGTDG
                     SGADQGPAHEVVEEIRAMGGEAVANGDNVAEWEGGRRLVQTAIDAFGRLDVLVNNAGI
                     LRDRVLVNMEEHEWDAVMAVHLKGHFVPTRFAAAYWREQVKAGEAVSGAVINTSSGAG
                     LFGNFGQTNYSAAKAGIAAFTITAAMELGRYGVRVNAIAPLARTRLTLQTPGLGDVVA
                     PPEAEDAFDVWHPGNISPLVAYLGTADCPFTGGVFHVGGREVGLVQNWTVGEVLSSPS
                     GTRWTVAELAAESKRMLEGRPPLASAGTTIDGLTKEFFVRHQG"
     gene            2828..3628
                     /locus_tag="VNU01_01970"
     CDS             2828..3628
                     /locus_tag="VNU01_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWH31418.1"
                     /translation="MAAEILYEADGHVATIWLNRPERANAATFEMAEQLREALTRADR
                     DRDVRVIVLSGKGKAFCSGDDVEQAWGDERMAETLRELADVRPPLTPEARVMLDVTKP
                     MIAAVNGAAVGIGMDFALLCDLRIASEHAKFGQLFVKLGLMADVTGLWRLPQLVGLEK
                     ATELLLTGDVVGAAEAERIGLVSRVVPAEELLPAAMDLARRIAANPPIAVRHIKEGLR
                     RGVGRSYGDLDELAVFVGNGLARLFATKDHREAAMAFVQKRPAVFTGE"
     gene            complement(3647..5233)
                     /locus_tag="VNU01_01975"
     CDS             complement(3647..5233)
                     /locus_tag="VNU01_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWH31419.1"
                     /translation="MADEIVMDEDEEIYGFWMRAAAEPGRRLLVEPDGTEHHAGELAA
                     ASNRLVHGLRDLGLARGDVVAVYLPNCAEVVELLMATQQGGWYLVPINHHLKPAEAAY
                     IVGDSGAKAFVTHARYADVALEVAALIDLPPQARFAVGAVDGFRPYTELRDPYPADAP
                     EDRAAGLLMTYTSGTSGRPKGVKRPLPEFDPDLQGEVQQIANTLFGLGDAGGVHLVTG
                     PMYHTAVIGLAMSALHAGNAVVLMDDWEPEQTLRLIERYRVSTTHMVPTMFNRLLKLP
                     AAVRERYDLSSLTQVTHGAAPCPVPVKHEMIRWFGPVISEYYAATEGGGTLVSSLEWL
                     ERPGTVGRPWQVSRIKITDDDGRELPPGEVGTIWIGMMVGGIENFEYHGDAGKTSLTF
                     DADGNFTVGDVGYLDDDGYLYICDRKIDMIISGGVNIYPAEVESVLAGHPAVLDVAVF
                     GVPDEDWGEQVKAAVELADGFTPSGELEQDLIAYAREHIAHLKCPRSIDFGPLPRDPN
                     GKVFKRVLADPYWQGRDRRV"
     gene            complement(5245..5733)
                     /locus_tag="VNU01_01980"
     CDS             complement(5245..5733)
                     /locus_tag="VNU01_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31420.1"
                     /translation="MPDDCVVGEVGRGWTLARTTLANERVAMSAGSALGGSLEQLVGL
                     AARVVGDDAVALDRLGRLVADEQTLSVMAHRTTLRQVSGTDPGPGASVRKLVSGLHQQ
                     DATEFGLELLGADGATTDGEAAAWSAMFLAMRAMTIAGGTTEVQRNLIAERLLGLPRD
                     DA"
     gene            5614..>6139
                     /locus_tag="VNU01_01985"
     CDS             5614..>6139
                     /locus_tag="VNU01_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31421.1"
                     /translation="MLQAAAERGARAHRDALVGQRRSRERPAAADLAHHAVVRHGYVQ
                     PHWPAPWGRDAGAVEQLVIAEEFRRAGVRPPVLMMGDWVLPTLIQYGDDAQKERFVLP
                     TLEGDIVWCQLFSEPGAGSDLAAVATKASRVDGGWLLNGQKVWNSLAAQADWAICIAR
                     TDPTVPKHEGITYFL"
BASE COUNT          781 a         2243 c         2232 g          883 t
ORIGIN      
        1 gcgatgacgc acaacctggg cggcgcgccc ggggagtgcg tgagcttcgt cagcatcgtc
       61 ggcagcgagc cctccgcctg atgggcgccg ggccgctcga cggcgtccgg gtcctggagc
      121 tcgccgcgat cggcccggcc cccttcggcg tgatgctgct ggcggacctg ggcgcggagg
      181 tgatccgggt cgaccgggtc gccaacgtgc gcacgcagac cggcccggag ttcggcatgc
      241 gcggggtcgg gcgcaaccgc cgctcggtcg gcatcgacgt gcgcacgccc gaggggcgcg
      301 acctggtcct ggcgctggcc gagcgcgccg acgtgctggt ggagggcttc cggccgggcg
      361 tcaccgaacg gctgggcgtc ggccccggcg actgcatggg gcgcaacccg cgcctggtgt
      421 acgcgcgcat gaccggctgg ggccaggacg ggcccctggc cccgacggcc ggccacgacc
      481 tcaactacgc cgccgtgggc ggcgcgatcc accccatcgg ccgcgcggac agccctcccc
      541 cgccgccgct caacttcctg gcggacttcg ccggcggcgg ctgctacctg gccctgggcg
      601 tgctggcggc gctggtcgag cgcgaccgct cgggccgggg gcaggtggtg gacgccgcga
      661 tggtggacgg cgtggtgtcg ttgacggcgt tcttccacgg cctgctgcgc atgggcgcgt
      721 ggaccaccga gcgcgaatcc aacctgctcg acggcggcac gccctggtac gacacctacg
      781 agaccgccga cggcggcttc atggcggtcg gcgcggtcga gccgcagttc tacgccgagc
      841 tgctggcgcg cctgggcctg gacgcgacgg actggccgca gcacgaccgc gagcgctggc
      901 ccgccatgcg caagacgttc acagagacgt tccggtcacg cacccggcag gagtggacgg
      961 cggtcttcga cggctccgac gcgtgcgtcg cgcccgtgct caccctcgcc gaggcgccgg
     1021 gccacccgca cctggtggcg cggcaggcct tcgtcgacgt ggccggcgtg cccgagcccg
     1081 cgcccgcccc acgattctcg cgcacgcccg gcaccgtccg gcgccccgcg ccggccttcg
     1141 gccaggacag cgacgaggtg ctggccgagt tcggcctgga cgccgcgcgc gtcgcggacc
     1201 tgcgcgcgcg cgacgtcgtg gcctgagctc agccgtcgag cttggcccgc aggccggcga
     1261 acaggtcctc gaccatgcgc ttggcgccct ggcggcgcag gaccgccggg atcttgatgg
     1321 ccggcgaggc ctccagctcg taggtcacgc gcgtgcgtcc gtcgccggcg tcggccagcg
     1381 tgtagcgccc gtcgagcgtc ttgaggatgt cgccctcgac ggcgtggaac gccacgccgt
     1441 cgtcgtcgta ggcgtaggcc agcgtgtagt tggcggtgaa catctgcccc ttgaacttgt
     1501 agcgcacgag cgtgccgcgg ccctcggcgt cgcgctcgag cacctcggcc tccgccatct
     1561 gcggctgcca gtccgggtag gactcgaagt cggtgacgat cgccaggatg ctcgccgggt
     1621 cgcccggcag ctcctcggtc tcctgcacgc ggtcactcat gctgcactcc tctcgccgct
     1681 gcggtcacgt ctgcgcgcgg tcgcgctcag ccttggtgcc ggacgaagaa ctccttggtg
     1741 agcccgtcga tcgtggtgcc ggcgctggca aggggcgggc ggccctccag catgcgcttg
     1801 ctctcggcgg cgagctcggc gaccgtccag cgcgtgccgg acggcgagga cagcacctcg
     1861 cccacggtcc agttctgcac cagcccgacc tcgcggccgc cgacgtggaa cacgccgccg
     1921 gtgaacgggc agtccgcggt gcccaggtag gcgaccagcg gcgagatgtt gccggggtgc
     1981 cacacgtcga aggcgtcctc cgcctccggc ggggcgacca cgtcgccgag gcccggggtc
     2041 tgcagcgtca ggcgcgtgcg ggccagcggc gcgatcgcgt tcacgcgcac gccgtagcgg
     2101 cccagctcca tcgccgcggt gatcgtgaag gccgcgatgc cggccttggc ggcggagtag
     2161 ttggtctggc cgaagttgcc gaacaggccg gcgcccgacg aggtgttgat cacggcgccg
     2221 gacacggcct cgcccgcctt gacctgctcg cgccagtacg ccgccgcgaa gcgcgtcggc
     2281 acgaaatggc ccttgaggtg gacggccatg acggcgtccc actcgtgctc ctccatgttg
     2341 accagcacgc ggtcgcgcag gatgccggcg ttgttcacca gcacgtccag gcggccgaag
     2401 gcgtcgatcg cggtctggac gaggcggcgg ccgccctccc attccgccac gttgtcgccg
     2461 ttggcgacgg cctcgccgcc catcgcgcgg atctcctcga ccacctcgtg cgcggggccc
     2521 tggtcggcgc cggagccgtc cgtgccgccg ccgaggtcgt tgacaaccac gcgggcgccc
     2581 tcctcggcga acagcagcgc gtgctcgcgg ccgatgccgc gtcccgcgcc ggtgatgatg
     2641 gcgacgcgtc cgtcaagagc tcccatggtt ggcggccttc tttcttgtca cggcccgcag
     2701 gcccagccta tgtttgtggc gattcacttt gtagcagatg cggggttggt gcggaagccc
     2761 gttgccggga ccggcgtcgc actgctacgt tattctcgac ttactcagtg cgacgtggga
     2821 gggcggcatg gctgcggaga tcctgtacga ggccgacggg cacgtcgcca cgatctggct
     2881 gaaccgcccg gagcgcgcca acgccgcgac cttcgagatg gccgagcagc tccgcgaggc
     2941 gctcacgcgc gccgaccggg accgcgacgt gcgggtcatc gtgctgtccg gcaagggcaa
     3001 ggcgttctgc tccggcgacg acgtcgagca ggcctggggc gacgagcgca tggcggagac
     3061 gctgcgcgaa ctcgccgacg tgcgcccgcc cctgacaccg gaggcccggg tgatgctcga
     3121 cgtcaccaag ccgatgatcg cggccgtcaa cggcgccgcg gtcggcatcg gcatggactt
     3181 cgccctgctg tgcgatctgc gcatcgcctc ggagcatgcc aagttcggcc agctgttcgt
     3241 caagctgggc ctcatggccg acgtcaccgg cctgtggcgg ctgccgcagc tggtgggcct
     3301 ggagaaggcg acggagctgc tgctcaccgg cgacgtcgtc ggcgccgccg aggcggagcg
     3361 catcggcctg gtgagccgcg tcgtgccggc agaggagctc ctgcccgccg cgatggacct
     3421 cgcgcggcgc atcgccgcca acccgccgat cgcggtgcgc cacatcaagg agggactgcg
     3481 ccgcggcgtc ggacgctcgt acggggacct ggacgagctg gccgtgttcg tcggcaacgg
     3541 cctggcgcgc ctgttcgcca ccaaggacca ccgcgaggcg gcgatggcgt tcgtgcagaa
     3601 gcgcccggcg gtgttcaccg gcgagtgagc gcgctccggc cgggggtcac acgcggcggt
     3661 cccggccctg ccagtacggg tcggccagga cgcgcttgaa caccttgccg ttggggtcgc
     3721 gcggcagcgg gccgaagtcg atcgagcgcg ggcacttcag gtgcgcgatg tgctcgcggg
     3781 cgtaggcgat caggtcctgc tccagctccc cggacggcgt gaagccgtcc gcgagctcca
     3841 cggccgcctt gacctgctcg ccccagtcct cgtccggcac gccgaacacc gcgacgtcca
     3901 gcaccgccgg gtgcccggcg agcacgctct cgacctcggc cgggtagatg ttcacgccgc
     3961 ccgagatgat catgtcgatc ttgcggtcgc agatgtacag gtagccgtcg tcgtccaggt
     4021 agcccacgtc gccaaccgtg aagttgccgt cggcgtcgaa ggtgagcgag gtcttgccgg
     4081 cgtcgccgtg gtactcgaag ttctcgatgc cgccgaccat catcccgatc cagatggtgc
     4141 cgacctcacc gggcggaagc tcccgcccgt cgtcgtcggt gatcttgatg cgcgacacct
     4201 gccacggtcg gcccacggtg ccgggccgct ccagccactc cagggaggac accagcgtgc
     4261 cgccgccctc ggtcgcggcg tagtactcgg agatcaccgg cccgaaccag cggatcatct
     4321 cgtgcttgac cggcaccggg cacggcgccg ccccgtgcgt gacctgggtg agcgaggaca
     4381 ggtcgtagcg ctcgcgcacc gccgccggca gcttcagcag ccggttgaac atcgtcggca
     4441 ccatgtgcgt ggtcgacacg cgataccgct cgatgaggcg cagcgtctgc tcgggctccc
     4501 agtcgtccat gagcaccacc gcgttgccgg cgtgcagcgc cgacatcgcc aggccgatca
     4561 cggccgtgtg gtacatcggg ccggtgacca ggtgcacgcc gcccgcgtcg cccaggccga
     4621 acagcgtgtt ggcgatctgc tgcacctcgc cctgcaggtc cgggtcgaac tcgggcagcg
     4681 gccgcttcac gcccttgggc cgcccgctgg tgccggaggt gtaggtcatg agcagcccgg
     4741 cggcgcggtc ctccggcgcg tcggccgggt aggggtcgcg cagctcggtg tagggccgga
     4801 agccgtccac ggcccccacc gcgaagcggg cctgcggcgg caggtcgatc agcgcggcga
     4861 cctccaaggc gacgtcggcg tagcgcgcgt gggtgacgaa cgccttcgcg cccgagtcac
     4921 ccacgatgta ggcggcctcg gcgggcttga ggtggtggtt gatgggcacc aggtaccagc
     4981 cgccctgctg ggtggccatg agcagctcga ccacctcggc gcagttgggc aggtacacgg
     5041 cgaccacgtc gccgcgcgcc aggccgaggt cgcgcagccc gtgcacgagg cggttggagg
     5101 cggcggcgag ctcgcccgcg tggtgctcgg tgccgtcggg ctccaccagc aggcgccggc
     5161 ccggctcggc ggccgcgcgc atccagaagc cgtagatctc ctcgtcctcg tccatcacga
     5221 tctcgtccgc catggccagt cctctcaggc gtcgtcgcgc ggcaggccga gcaggcgctc
     5281 ggcgatcagg ttgcgctgca cttcggtcgt gccgccggcg atggtcatgg cgcgcatcgc
     5341 caggaacatc gcgctccagg cggccgcctc gccgtcggtg gtggcgccgt ccgcgccgag
     5401 cagctccagc ccgaactcgg tggcgtcctg ctggtgcagg ccgctgacca gcttgcgcac
     5461 gctggcgccc ggcccggggt cggtgccgct cacctgccgc agcgtggtgc ggtgcgccat
     5521 gaccgacagc gtctgctcgt cggccaccag gcggcccagc cggtccagcg ccaccgcgtc
     5581 gtcaccgacc acgcgcgccg ccaggcccac cagctgctcc aggctgccgc cgagcgcgga
     5641 gcccgcgctc atcgcgacgc gctcgttggc cagcgtcgtt cgcgcgagcg tccagccgcg
     5701 gccgacctcg cccaccacgc agtcgtccgg cacggctacg tgcagccgca ctggccggcg
     5761 ccgtgggggc gcgacgccgg cgcggtcgag cagctcgtca tcgccgagga gttccgccgc
     5821 gccggcgtgc gtccgcccgt gctgatgatg ggcgactggg tgctgcccac gctgatccag
     5881 tacggcgacg acgcgcagaa ggagcgcttc gtgctgccga cgctggaggg cgacatcgtc
     5941 tggtgccagc tcttcagcga gcccggcgcc ggctccgacc tcgcggcggt ggccaccaag
     6001 gcgtcgcgcg tcgacggcgg ctggctgctc aacggccaga aggtgtggaa ctccctggcc
     6061 gcgcaggcgg actgggcgat ctgcatcgcg cggaccgatc ccaccgtgcc caaacacgag
     6121 ggcatcacct acttcctgg
//
LOCUS       DATMKC010000069         4327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_183879, whole genome shotgun sequence.
ACCESSION   DATMKC010000069 DATMKC010000000
VERSION     DATMKC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183879"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="bioF"
                     /locus_tag="VNU01_01990"
     CDS             complement(<1..1124)
                     /gene="bioF"
                     /locus_tag="VNU01_01990"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWH31422.1"
                     /translation="MPEPLDWVAGELARLEAAGLRRRLRRRSSPAGRTADGLVNLSSN
                     DYLDLAGDPRLAIAAAEAARDWGAGSGASRLVTGTTALHTQLEREIAAWKGTQDAVVF
                     SSGYLANVGTIQALVGRGDTVCSDALNHASIIDGCRLSRADVRVFAHADADALDRALA
                     GAAGRKLVVTDGVFSMDGDAAPLRELCDVAEAHGAMVMVDDAHGCGVTGPDGRGTAAA
                     QGAEHRVHVQLGTLSKAFGAAGGYVAGSAELCDWLRNRARSFVFDTAPPPPVVGAALE
                     GLRVARAEPHRRERATALAQRLAGALRLPSPAAAVVPFVVGTPDAALAAQAELEHAGL
                     LVTAIRPPTVPDGSSRLRFALTAAHTEDDVDRAADVLSGRG"
     gene            complement(1117..2481)
                     /gene="bioA"
                     /locus_tag="VNU01_01995"
     CDS             complement(1117..2481)
                     /gene="bioA"
                     /locus_tag="VNU01_01995"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HWH31423.1"
                     /translation="MHSPGSGDSARLDLQHVWHPFTPMHLWLEEDPLVIERAEGCWLF
                     DTDGNRYLDGNSSLWVNVHGHRKPELDAALKAQVDKVAHTTYLGLGNPAASQLAARLV
                     ELAPPGLARVFFSESGASAVEVALKIAFGYWRNIGERQRDLFVSMDGAYHGDTLGAVS
                     VGHMSDFHDKYRPLLFPTDAFAQPYCYRCPLGLEHPACDLACADTLDDVLARHGDRVA
                     AVIVEPLVQGAAGIVTAPDGHLARVAEIARRHGTLLIVDEIATGFGRTGAMFACDLEG
                     VSPDLMTVGKGLTGGYLPMSATLTTQAIFDAYARAEDTFFHGHSYSGNPLSAAVALAN
                     LDVFAAEDTVGRARALGEVLEAELKQLADLAHVGDVRRRGIMAGVELVADRATRAPYD
                     PAARMGAAVCRRARDLGLVIRPLGDTIVVMPAPAMSADELRFLVATLARAIRDVTEDG
                     GGGA"
     gene            2578..4020
                     /locus_tag="VNU01_02000"
     CDS             2578..4020
                     /locus_tag="VNU01_02000"
                     /inference="COORDINATES: protein motif:HMM:NF005883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HWH31424.1"
                     /translation="MPVLRIVIVGGGPGGYEAALVAAEAGAQVTVVTAEPTLGGNSVL
                     WDSVPSKTLIVSAEAMGWAQSAHRLGVRLESGEALAGQARVDLGAVMDRVRSLAGTQS
                     ADITAKVEAAGVAVVRGTARLAGRGAVEVEEDGGATRVLPADVVLIATGSNPRTLPFS
                     VPDGERVFTSRELGNLRELPERLIVVGSGATGAEYAHAFARFGAEVHLISSRDQVLPN
                     EDADAAAVIEGSFERWGMVIHRRTRAVDLERSADGVRLKVTRRATPDAEPQEEWVEGS
                     HALFCIGQVPSSAKLGLEAAGVELAEGGAIPVDGVSRTNVPTIYAAGDVTGGIMLASV
                     AAMQGRNAMWHALGQAVAPLRRHTIAACIFTDPEVASVGITEAQARERQLPFETVSLT
                     YAGNPRAKMAENTDGFVKLHAMEGSGTVLGGVVVADRASDLITPVSVAVANGLSVAQL
                     AHAFSIYPSMSGSLQETARQLMGRLASPWT"
     gene            complement(4078..>4327)
                     /locus_tag="VNU01_02005"
     CDS             complement(4078..>4327)
                     /locus_tag="VNU01_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HWH31425.1"
                     /translation="PCIGALRTSVAPMQGTIVKTAVEEGQTVSAGDLVLVLEAMKMEN
                     HISAHRDGVVTALHVSAGDVVNSGDKLATIEDAPAAES"
BASE COUNT          554 a         1583 c         1639 g          551 t
ORIGIN      
        1 ccccggcccg acagcacgtc ggcggcgcgg tcgacgtcgt cttcggtgtg cgcggcggtc
       61 agcgcgaatc gcaggcgcga gctgccgtcg ggcaccgtcg gcggccggat cgccgtgacc
      121 aagagccccg cgtgctccag ctcggcctgc gcggccagcg ccgcgtccgg ggtgccgacc
      181 acgaacggca cgaccgccgc cgcggggctc ggcagtcgca gtgcgccggc cagccgctgg
      241 gccagcgcgg tggcgcgctc gcggcggtgc ggctcggcac gcgccacgcg caggccctcc
      301 agcgccgcac ccaccaccgg gggcggcggg gccgtgtcga agacgaagct gcgcgcgcgg
      361 ttgcgcagcc agtcgcacag ctcggcgctg cccgccacgt agccgcccgc ggcgccgaac
      421 gccttggaaa gggtgccgag ctgcacgtgc acgcggtgct cggcgccctg cgccgcggcg
      481 gtgccgcgcc cgtccgggcc ggtcacgcca cagccgtgcg cgtcgtcgac catcaccatg
      541 gcgccgtgcg cctcggcgac gtcgcagagc tcgcgcaggg gggcggcgtc gccgtccatg
      601 gagaacacgc cgtccgtgac gaccagcttg cgaccggcgg cgcccgccag tgcccggtcg
      661 agcgcgtcgg cgtcggcgtg ggcgaacacg cgcacgtccg ctcgggacag gcggcagccg
      721 tcgatgatcg aggcgtggtt cagcgcgtcg ctgcacactg tgtcgccgcg gccgaccagg
      781 gcctggatgg tgccgacgtt cgccaggtag ccggaggaga acaccaccgc gtcctgcgtg
      841 cccttccacg cggcgatctc gcgctccagc tgcgtgtgca gcgccgtggt gccggtgacc
      901 aggcgtgagg cgccggaccc ggcgccccag tcgcgcgccg cctcggcggc ggcgatcgcc
      961 aggcgcgggt cgcccgccag gtccaggtag tcgttgctgg acaggttgac caggccgtcg
     1021 gccgtgcgcc cggcggggga ggagcgccgg cgcagccgcc gccgcaaccc cgcggcctca
     1081 agtctcgcga gttccccggc cacccagtcc agcggctcag gcaccgccgc cgccgtcctc
     1141 ggtcacgtcg cggatcgccc gcgccagcgt ggccaccagg aagcgcagct cgtcggcgct
     1201 catcgccggc gcaggcatga ccacgatggt gtcgcccagc ggccggatca ccaggccgag
     1261 gtcgcgtgcc cgccggcaca ccgccgcgcc catgcgcgcc gccgggtcgt agggcgcgcg
     1321 ggtggcgcgg tcggcgacca gctccacgcc cgccatgatg ccgcgccggc gcacgtcgcc
     1381 gacgtgggcc aggtcggcca actgcttgag ctcggcctcc agcacctcgc ccagggcacg
     1441 ggcgcgcccc acggtgtcct cggcggcgaa cacgtccagg ttcgccagcg ccacggccgc
     1501 gctcagcggg ttgccggagt agctgtggcc gtggaagaag gtgtcctcgg cgcgggcgta
     1561 ggcgtcgaag atcgcctggg tggtcagggt cgccgacatc ggcaggtagc cgccggtcag
     1621 gcccttgccc acggtcatga ggtccgggga cacgccctcc aggtcgcagg cgaacatcgc
     1681 gcccgtgcgg ccgaagccgg tggcgatctc gtcgacgatg agcagcgtgc cgtggcggcg
     1741 ggcgatctcg gccacgcgcg ccaggtggcc gtccggcgcg gtgacgatgc cggcggcgcc
     1801 ctgcaccagc ggctccacga tcacggcggc gacgcggtcg ccgtggcggg cgagcacgtc
     1861 gtcgagcgtg tccgcgcacg cgaggtcgca ggccgggtgc tccaggccca gcgggcagcg
     1921 gtagcagtac ggctgcgcaa aggcgtcggt cggaaacagc agcggccggt acttgtcgtg
     1981 gaagtcgctc atgtgcccga cgctgacggc gcccagcgtg tcgccgtggt acgcgccgtc
     2041 catggacacg aacaggtcgc gctgccgctc accgatgttg cgccagtagc cgaaggcgat
     2101 cttcagcgcg acctcgaccg cgctggcgcc ggactcggag aagaacacgc gggccagccc
     2161 ggggggcgcc agctccacga ggcgcgcggc gagctgggag gccgcggggt tgcccaagcc
     2221 caggtacgtg gtgtgcgcga ccttgtcgac ctgcgccttc agcgcggcgt ccagctccgg
     2281 cttgcggtgc ccgtgcacgt tgacccacag cgagctgttg ccgtcgaggt agcggttgcc
     2341 gtcggtgtcg aacagccagc agccttccgc ccgctcgatg acgagcgggt cctcctccag
     2401 ccagaggtgc atcggtgtga agggatgcca gacgtgctgg aggtcgaggc gcgcggagtc
     2461 gccggaaccg gggctgtgca cgccgatcag ggtgcgcggg ggcgactggc cggtcaaatc
     2521 tccgtgcgcc tcgcgcagtg aggatgaaat cgggcgcggc ggcgtaggct ccggcacatg
     2581 cctgtgctgc gcatcgtcat cgtcggcggc ggaccgggcg gctacgaggc cgcgctcgtg
     2641 gccgcggagg ccggcgccca ggtcaccgtc gtcaccgccg agcccacgct gggcggcaac
     2701 agcgtcctgt gggactccgt gccgtccaag accctgatcg tgtccgcgga ggcgatgggc
     2761 tgggcgcagt ccgcgcaccg cctcggcgtg cggttggaga gcggcgaggc gctggcgggc
     2821 caggcccgcg tggacctcgg tgccgtcatg gaccgggtgc gcagcctggc cggcacgcag
     2881 tcggcggaca tcaccgccaa ggtcgaggcc gccggcgtgg cggtcgtgcg cggcacggcc
     2941 cggctggccg ggcgcggcgc ggtggaggtg gaggaggacg gcggcgccac gcgcgtgctg
     3001 ccggcggacg tggtgctgat cgccacgggc tccaacccgc gcaccctgcc gttctccgta
     3061 ccggacggcg agcgcgtctt cacctcccgc gagctgggca acctgcgcga gctgcccgag
     3121 cggctgatcg tcgtcggctc cggtgccacg ggggccgagt acgcgcacgc gttcgcgcgc
     3181 ttcggcgccg aggtgcacct gatctccagc cgcgaccagg tcctgcccaa cgaggacgcc
     3241 gacgcggccg ccgtcatcga gggcagcttc gagcgctggg gcatggtgat ccaccgccgc
     3301 acgcgcgccg tggacctgga gcgctcagcc gacggcgtgc gcctgaaggt gacgcgccgg
     3361 gcgacgccgg acgccgagcc gcaggaagaa tgggtcgagg gcagccacgc gctgttctgc
     3421 atcggccagg tgccgtcgtc ggccaagctg ggcctggagg cggcgggcgt cgagctcgcc
     3481 gagggcggcg ccatccccgt cgacggcgtg agccgcacga acgtgccgac catctacgcg
     3541 gccggcgacg tgaccggcgg catcatgctc gccagcgtcg cggcgatgca ggggcgcaac
     3601 gcgatgtggc acgcgctcgg gcaggcggtg gcgccgctgc gccgccacac catcgcggcg
     3661 tgcatcttca ccgacccgga ggtcgcctcc gtcggcatca ccgaggcgca ggcgcgcgag
     3721 cggcagctgc cgttcgagac ggtgtcgctg acctacgccg gcaacccgcg cgcgaagatg
     3781 gccgagaaca ccgacggctt cgtgaagctg cacgcgatgg agggctccgg gaccgtgctc
     3841 ggcggcgtcg tggtcgccga ccgcgccagc gacctcatca cgccggtcag cgtcgccgtc
     3901 gcgaacgggc tgagcgtcgc gcagctcgcc cacgcgttct cgatctaccc gtccatgtcg
     3961 ggctccctgc aggagacggc ccgccagctc atgggccgcc tggcgtcgcc ctggacctag
     4021 ccccccgccc gcctgcggag tcactcgcgg ccgatagtgg ccggaatcga ctccgcatca
     4081 gctttcggcg gcgggggcgt cctcgatggt ggccagcttg tcgccgctgt tgacgacgtc
     4141 gcccgcggac acgtgcagcg ccgtcaccac accgtcgcga tgcgcggaga tgtggttctc
     4201 catcttcatc gcctccagca ccaggaccag gtcgccggcg ctgaccgtct ggccctcctc
     4261 gacggcggtc ttgacgatgg tgccctgcat cggcgcgacg gaggtcctca gcgcgccgat
     4321 gcagggc
//
LOCUS       DATMKC010000070         2816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_305453, whole genome shotgun sequence.
ACCESSION   DATMKC010000070 DATMKC010000000
VERSION     DATMKC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305453"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VNU01_02010"
     CDS             <1..311
                     /locus_tag="VNU01_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWH31426.1"
                     /translation="PEAAVEAGGVRVDAERHEVRVRGEVVALPPKEFALLELLVRNAG
                     RVLTRDVLIDRVWGSDYVGDTKTLDVHVKRLRGRVEPDPRAPSLIVTVRGVGYKFADA
                     "
     gene            313..1527
                     /locus_tag="VNU01_02015"
     CDS             313..1527
                     /locus_tag="VNU01_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH31427.1"
                     /translation="MGVRRLVFLLIGAGVAAELAAAAWPGARWAVLAVFLAAASLLAW
                     AALQDEQRRRRTEVRRLEDSLERRISELATERHRIERLLERLPIAVLVFAPGGLAYGN
                     PAARALFPAAEEGRSPLRVLTLRALADAVAEARESGRTVHVEVERDERWLSARAVVMA
                     EGEVALVVTDQTEARRVEAVRRDFVVNASHELKTPVATMQVLAETLEMALSRDPATAA
                     GMAQRLRAEAVRMGQLVRDLLDLARMEEQGAERRERVELAGLVAGEVARVATDARALQ
                     VEVSADVGDAVSVVAVPEDVRLIVANLLENAVRYNRPGGRVLVRAARAGADVVIEVVD
                     TGIGLAEADRVRVFERFYRVDRARSRDAGGTGLGLALVRHAAERHGGEVSVDSVLGEG
                     STFRVILPVSAD"
     gene            1558..2295
                     /gene="nth"
                     /locus_tag="VNU01_02020"
     CDS             1558..2295
                     /gene="nth"
                     /locus_tag="VNU01_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWH31428.1"
                     /translation="MPTTPPDPTTLDRRSPKTKRGPVIVETLLETYEGGVVELDHGNA
                     FELLIATILSAQSTDKKINEITKTLFAKYRTPRDYLAVPEEELQQDVYQSGFYRQKTK
                     SIRGMCQALIERYGGEVPLSMAELVTLPGVARKTANVVLGAVAPERHREDPDAGIAVD
                     THVKRLSRRFGLTTHEDPEKIERDLMRLLPKQQWPRASLGIILHGRRVCDARRPRCGD
                     CVVEPLCPSSLVAGCKDKAGQAVGLGS"
     gene            complement(2292..>2816)
                     /locus_tag="VNU01_02025"
     CDS             complement(2292..>2816)
                     /locus_tag="VNU01_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31429.1"
                     /translation="PHAREGSREQAPRSSRAAHVGSTELYDCPTTWDGRAVRFTGEVV
                     GGVLQRGTHAWLQLNDDVYGGEPGPLPFHRELRGPNAGVGVLVPLEFIGAITHVGGPA
                     VQGDRVEVEGFFHRVDRESREVGVIRAETLKVVSAGQKLPRDVEPRRVLVAALLAVIA
                     AALLWLQWRARRRR"
BASE COUNT          367 a          989 c         1099 g          361 t
ORIGIN      
        1 agccggaggc cgcggtggag gcgggcggtg tgcgcgtcga cgcggagcgc cacgaggtgc
       61 gggtgcgcgg cgaggtggtc gcgctgccgc cgaaggagtt cgcgctgctg gagctgctgg
      121 tgcgcaacgc cgggcgggtg ctcacccggg acgtgctgat cgaccgcgtg tggggctcgg
      181 actacgtcgg cgacacgaag acgctggacg tgcacgtgaa gcggctgcgc ggtcgcgtcg
      241 agcccgaccc gagggcgccg tcgctgatcg tcacggtgcg cggcgtgggc tacaagttcg
      301 cggacgcctg acgtgggggt gcgccggctc gtcttcctgc tcatcggcgc cggcgtggcg
      361 gcggagctcg ccgccgcggc gtggccgggc gcgcgctggg cggtcttggc ggtgttcctg
      421 gcggcggcgt ccctgctggc gtgggcggcc cttcaggacg agcagcgccg gcggcgcacc
      481 gaggtgcgcc ggctggagga ctcgctggag cggcggatct ccgagctcgc cacggagcgc
      541 caccgcatcg aacggctgct ggagcgcctg cccatcgcgg tcctggtgtt cgcccccgga
      601 gggcttgcgt acgggaaccc ggcggctcgc gcgctgttcc cggcggctga ggagggccga
      661 tcgccgctgc gggtgctgac gctgcgcgcg cttgccgacg ccgtcgcgga ggcgcgcgag
      721 agcgggcgca ccgtgcacgt ggaggtggag cgcgacgagc gctggctgtc cgcgcgcgcc
      781 gtggtgatgg ccgaggggga ggtcgcgctg gtggtgaccg atcagaccga ggcccggcgc
      841 gtggaggcgg tgcgccgcga cttcgtggtg aacgcgtccc acgagctcaa gacgccggtc
      901 gccaccatgc aggtcctggc cgagacgctg gagatggcgc tgtcgcgcga tcccgccacg
      961 gcagccggca tggcccagcg cctgcgcgcg gaggccgtgc gcatggggca gctggtgcgc
     1021 gatctgctgg acctggcgcg catggaggag cagggcgccg agcgccgcga gcgggtggag
     1081 ctggcggggc tcgtggccgg cgaggtcgcg cgggtggcca cggacgcacg ggctctccag
     1141 gtggaggtga gcgccgacgt gggcgacgcc gtgtcggtgg tggccgtccc ggaggacgtc
     1201 cgcctcatcg tcgcgaactt gctggagaac gccgtgcgct acaaccggcc gggcggccgc
     1261 gtgctggtcc gggcggcgcg cgccggcgcc gacgtggtga tcgaggtggt ggacaccggc
     1321 atcggcctgg cggaggcgga ccgcgtccgc gtgttcgagc gcttctaccg cgtcgaccgc
     1381 gcgcgcagcc gcgacgccgg cggcaccggt ctcgggctgg ccctggtgcg ccacgccgcg
     1441 gagcgccacg gcggcgaggt gagcgtcgac agcgtcctgg gggagggcag cacgttccgg
     1501 gtgatcctgc ccgtcagcgc cgactgagcg ccgcggccac gcacctacac tctcgccatg
     1561 ccgacgaccc cgcccgatcc cacgacgctg gaccgccgca gtcccaagac caagcgcggc
     1621 ccggtcatcg tggagacgct gctggagacg tacgagggcg gcgtggtgga gctcgaccac
     1681 ggcaacgcct tcgagctctt gatcgcgacg atcctgtcgg cgcagtcgac cgacaagaag
     1741 atcaacgaga tcaccaagac gctgttcgcc aagtaccgca cgccgcggga ctacctggcg
     1801 gtgcccgagg aggaactcca gcaggacgtc taccagtccg gcttctaccg gcagaagacc
     1861 aagagcatcc gggggatgtg ccaagcgctg atcgagcgct acggcggcga ggtcccgctg
     1921 tcgatggccg agctggtgac gctgccgggc gtggcgcgca agaccgccaa cgtggtgctg
     1981 ggcgcggtcg cccccgagcg ccaccgcgag gacccggacg ccggcatcgc cgtcgacacg
     2041 cacgtcaagc gcctgtcccg ccgcttcggg ctgaccacgc acgaggaccc ggagaagatc
     2101 gagcgcgacc tcatgcgcct gctgcccaag cagcagtggc cgcgggcgag cctgggcatc
     2161 atcctgcacg gccgccgcgt ctgcgacgca cgccgcccac gctgcggcga ctgcgtcgtc
     2221 gagccgctgt gcccgtcgtc actggtggcc ggctgcaagg acaaggccgg ccaggccgtg
     2281 ggcctgggct cctagcgccg ccgccgcgcg cgccactgca gccacagcag cgcggcggcg
     2341 atcaccgcca gcagcgcggc gaccaggacg cgcctgggtt ccacgtctct gggcagcttc
     2401 tggccggctg acaccacctt cagcgtctcc gcgcggatga cgccgacctc gcgggactcg
     2461 cggtcgacac ggtggaagaa cccctcgacc tcgacgcggt ccccctggac ggccggcccg
     2521 cccacatggg tgatcgcgcc gatgaactcc agcggcacca gcacgcccac cccggcgttc
     2581 ggcccgcgga gctcgcggtg gaacggcagg gggccgggtt cgccgccgta gacgtcgtcg
     2641 ttgagctgca gccatgcgtg cgtgccccgc tggagcacgc cgcccacgac ttcgccggtg
     2701 aaccgcacgg cgcggccatc ccaggtggtc gggcagtcgt acagctccgt ggagccgacg
     2761 tgcgccgcac ggctggagcg cggtgcctgc tcccgcgacc cttcgcgcgc gtgcgg
//
LOCUS       DATMKC010000071         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_14672, whole genome shotgun sequence.
ACCESSION   DATMKC010000071 DATMKC010000000
VERSION     DATMKC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14672"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1810)
                     /locus_tag="VNU01_02030"
     CDS             complement(116..1810)
                     /locus_tag="VNU01_02030"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HWH31430.1"
                     /translation="MALDVEIGWFSARGPREHNEDFVAALRPAAHEAARGLVAAIADG
                     VSQGGGGREAAQTTVMTLLQDYFATPDTWDTSVALDRVIGAQNAWLAGRNRRNPGQAA
                     MTTLTAIVLRGHSYTLAHVGDSRAWLLRDGRCEPLTHDHVFDHSDVRSRVTRAIGLDE
                     HVHPDYSSGELRPGDVFVLTTDGVHGTLRGSAIAAHVGGDDAQAASEGIVRAAVAAGS
                     RDNASALVIRVRELDGADLRDGLLLARSLPALPRLRVGDELDGFVITGLLRDTGVHRL
                     YQAREAASGRLVVLKAMHEARANDPEERAMLAHEAWLGQRLADVPGFVAAREHAGASA
                     LYAVFEWHPGTTLQQMLDRGRAFGVAQVVEGAVAVAGALGRLHRLGVVHRDIKPANLH
                     LGDDGVWRILDLGAAISGRESPEQRALHAGTPSYMNPEQWEEGAGATPQSDLFALGVT
                     LYQWLTGHLPYGEVEPYQRARYRRDPKAPSRLRPDVPIWLDHIVLKAVALDARQRFET
                     AEELLLALERGASRTLHAPHATPLIARDPATLWKVALAVSAALNLLLVFWLLFLPR"
     gene            1876..>2076
                     /locus_tag="VNU01_02035"
     CDS             1876..>2076
                     /locus_tag="VNU01_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH31431.1"
                     /translation="MKKSKLVVVGNGMAGVRAIEELLKIAPQLYDVTVFGAEPHPNYN
                     RILLSPVLAGEQTLDEIVLNSWE"
BASE COUNT          283 a          758 c          759 g          276 t
ORIGIN      
        1 atgcagatcc gctggacccg cacctgggca gagaagggag gccgtgcccg gcacgtcccc
       61 gcccccgcgg ccgcccccgc taccgggccc ctgcaacgga gggcctgaag tgagctcacc
      121 ggggcaggaa cagcagccag aacaccagca ggaggttgag cgccgccgag acggcgagcg
      181 ccaccttcca cagggtcgcc ggatcgcgcg cgatcagcgg cgtggcgtgc ggcgcgtgga
      241 gcgtgcgcga ggcgccgcgc tccagcgcga gcagcagctc ctcggcggtc tcgaagcgct
      301 ggcgcgcatc gagcgccaca gccttgagca cgatgtggtc cagccagatc ggcacgtcgg
      361 gccgcagccg ggacggcgcc ttggggtcgc gccggtagcg cgcgcgctgg tacggctcga
      421 cctccccgta cggcaggtgg ccggtgagcc actggtacag cgtgacgccc agggcgaaca
      481 ggtcgctttg cggggtcgcg ccggcgccct cctcccactg ttcgggattc atgtagctcg
      541 gcgtgccggc gtgcagcgcg cgctgctcgg ggctctcgcg gccggaaatc gccgcgccca
      601 ggtccaggat gcgccacacg ccgtcgtcac cgaggtgcag gttggcgggc ttgatgtcgc
      661 gatggacgac gccaaggcgg tgcaggcggc ccagggcacc cgcgacagca accgcgccct
      721 ccaccacctg cgccacgccg aaggcccggc cgcgatcgag catctgctgc aaggtcgtgc
      781 cgggatgcca ctcgaacacc gcgtagagcg cgctcgcgcc ggcgtgctcg cgcgcggcca
      841 cgaagccggg aacgtcggcg agccgctgcc cgagccaggc ctcgtgcgcc agcatcgcgc
      901 gctcctccgg gtcattggcg cgggcctcgt gcatggcctt gagcacgacg aggcggccgc
      961 tcgcggcctc gcgcgcctgg tacagccggt gcacgccggt gtcgcgcagg aggccggtga
     1021 tgacgaagcc gtccagctcg tcgcccacgc gcagccgcgg cagggcgggc aggctgcgcg
     1081 cgagcagcag gccatcgcgc aggtcggcgc cgtcgagttc gcgcacccgg atgacgagcg
     1141 cgctggcgtt gtcgcggctg ccggcggcga ctgccgcccg cacgatgccc tcgctcgccg
     1201 cctgggcgtc gtcgccaccc acgtgcgccg cgatcgccga gccacgcagc gtgccgtgca
     1261 cgccgtcggt ggtcagaacg aacacgtcgc ccgggcgcag ttcgcccgag ctgtagtcgg
     1321 ggtggacgtg ttcgtccagg ccgatcgcgc gcgtgacgcg gctgcgcacg tcggagtggt
     1381 cgaacacgtg gtcgtgcgtg agcggctcgc agcggccgtc ccgcagcagc caggcccggc
     1441 tgtcgccgac gtgggccagc gtgtagctgt ggccgcgcag gacgatggcc gtcagcgtgg
     1501 tcatcgccgc ctggccgggg ttgcggcggt tgcggcccgc cagccaggcg ttctgcgcgc
     1561 cgatgacgcg gtccagcgcc acgctggtat cccaggtgtc cggcgtggcg aagtagtcct
     1621 gcagcagcgt catcaccgtg gtctgcgccg cctcgcgccc gccgccgccc tgcgacacgc
     1681 cgtcggcgat cgcggcgacc agcccgcgcg cggcttcgtg cgcggcgggt cgcagcgccg
     1741 ccacgaagtc ctcgttgtgt tcgcgcggac cgcgcgcgct gaaccagccg atctccacgt
     1801 ccagtgccat cgcgccatcg tagccgcggg cacggcgttt ccctttcccg tttgcacctg
     1861 gcaaggagtc cagccatgaa gaaatcgaaa ctggtcgtcg tcggcaacgg catggccggc
     1921 gtgcgcgcga tcgaggagct gctcaagatc gcgccgcagc tgtacgacgt gacggtgttc
     1981 ggcgccgagc cgcaccccaa ctacaaccgc atcctgctct cgcccgtgct ggccggcgag
     2041 cagacgctgg acgaaatcgt gctgaactcc tgggag
//
LOCUS       DATMKC010000072        12467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_236281, whole genome shotgun sequence.
ACCESSION   DATMKC010000072 DATMKC010000000
VERSION     DATMKC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236281"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VNU01_02040"
     CDS             complement(<1..849)
                     /locus_tag="VNU01_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWH31432.1"
                     /translation="MDHTGAGHRGPDGLTLVTFAVATTLGASNFIAVRFSNRELPPFW
                     GAGLRFGLAAALFVAICLALRLRRPRGGDLLLTAVYGALAFALSYALMYWALVRVTAG
                     MATIVLAVVPLMTLLLAAAQGSERLSPRSAAGALLALAGIAWMTLGPGAAAVPLDALA
                     AMAAAALAIAQSVILGKRLSGNHPAMTNAVGMTTGAVLLLGLSLAAGEAWAVPSGRST
                     LLALGYLVTFGSAGLFVLVLLVVRRWTASASSYMFVLFPVVTLALGALLAGEPVTRDA
                     VLGAVLV"
     gene            928..1143
                     /locus_tag="VNU01_02045"
     CDS             928..1143
                     /locus_tag="VNU01_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31433.1"
                     /translation="MWSEPIARLPERVCFTLAETGRLLFSIDVAVGLTAPESSGGRLA
                     RAAQRVVTRRLWPELGRLLEDHDGEEA"
     gene            1154..1345
                     /locus_tag="VNU01_02050"
     CDS             1154..1345
                     /locus_tag="VNU01_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31434.1"
                     /translation="MTTAERHVSVPEAAELLGMDGGELYLMIFRGGIRATPTLEQGVL
                     VPLDEVERLARERRADDGR"
     gene            complement(1342..2196)
                     /locus_tag="VNU01_02055"
     CDS             complement(1342..2196)
                     /locus_tag="VNU01_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31435.1"
                     /translation="MKLALATSAEHHDLIEDDQPLRKSLTERGADVTAAVWDDEGVDW
                     SSFDLVVIRSTWDYWHRLDEFTAWVDRVGGLTRLENPPNVVKWNAHKGYLDELQARGV
                     PVVPTETLARGSSHELGAVLRRRGWGEAVVKPAVSAGSQGTVRLRLREAAEHQAHLDG
                     LLAQGDVLVQPFVESVTQGGERSLVFFDGRYSHALRKQAAEGDFRVQPQFGGVITPDQ
                     PPQRVKDLARSILEAAEVEPLYARVDLVRIGERWKLIELELIEPLLYFHTDAKSVHRF
                     VDAILRRA"
     gene            complement(2207..2731)
                     /locus_tag="VNU01_02060"
     CDS             complement(2207..2731)
                     /locus_tag="VNU01_02060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH31436.1"
                     /translation="MSAGPAAPGAAPQGQEPDPRLIALWQETDAAGFLRAVSDGRVPR
                     SPHQEHAGLRIGAVEPGHVELEWRPEPHICNRAGIVHGGYLAIVLDDAAGLAGASLGE
                     RFVPMLTMDLRIEYIRPALPHQAYRAIGTVVHAGKTRVIADGRVEDGDGRMLARASGS
                     FTPNRAFLPERFQS"
     gene            complement(2728..3420)
                     /locus_tag="VNU01_02065"
     CDS             complement(2728..3420)
                     /locus_tag="VNU01_02065"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWH31437.1"
                     /translation="MAITLQRDGDFAHLSMDDGKANAFDLGFFTELDAALDDCGDAAA
                     VILRGRDGMFSGGLNLKVLQGSTPEALLELLSRFGRTMHRVWLEPRPVVAACTGHSVA
                     GGTILAMAADHAVAARGEFRWGLNETAIGMVMPEWILAIARANVRADRYEDLILSGAL
                     ISPEAAVEAGFADELADPADVVAAAEAKAAALAALPRHAYAATKRRLRAAASEASLAV
                     MEADLRAALGMA"
     gene            complement(3486..3959)
                     /locus_tag="VNU01_02070"
     CDS             complement(3486..3959)
                     /locus_tag="VNU01_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWH31438.1"
                     /translation="MAALRLTSPAFEHDDPLPARYTADKDGVSPPLAWEGVPPDAKEL
                     VLICDDPDGEEGVVTHWVVYGILPDTTGLPEGIPDDAVVEDDEVSIVQGLNEFDEVGY
                     TGPSVDADAGPHRIFFRLFALDVETTDLQPGITRAELRKAVKEHIIAQAELVGIV"
     gene            complement(3967..5373)
                     /locus_tag="VNU01_02075"
     CDS             complement(3967..5373)
                     /locus_tag="VNU01_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWH31439.1"
                     /translation="MTPSGIGAHREAYASGECTPVEVAERFLAAQPVGGDVDAFVSVD
                     PDALLAEAAASARRLRDGAPSGPLEGVLVGVKDFFAVRGHVTRGGTSFLAGDAQPEAE
                     LVTRLRRAGAIIAGKNRTTELGLSPIGVNPSGGSPRNPHDRERPCGGSSSGGAAAVAS
                     GMVPLAVGTDGGGSLRIPPAVCGVLGLKPTFDRVPMGGELNVGWWSVDVAGPIARTSA
                     DLAEAYAILADVAVPDLSAGLDGVVVGVDWDWWGAPDRAIDQACRPIAEGLAAKPVTL
                     AHMDLVRVAEYVTIGAEVAAALYDDLRDHPERFGDDVQAVLGAALEIPAVDYLRAQQA
                     RTLIDRAFGAVFAEVDVIVSPTVASTVPRLPDAVWRDGLLDEPLLRRLTAYTFPANLV
                     GLPAVTVPVGTDADGMPIGLQIMAARGREDLVLRAAAALERDGACAVPRPATWFDPLP
                     QAEGQATGGAPEAHHDRR"
     gene            complement(5370..5564)
                     /locus_tag="VNU01_02080"
     CDS             complement(5370..5564)
                     /locus_tag="VNU01_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31440.1"
                     /translation="MTGPVLRGVARATRAPLVGGGMRAVSLQLMGVTALRRARLGDVP
                     PYVAELTRPGRLAEGEEPTA"
     gene            complement(5618..7048)
                     /locus_tag="VNU01_02085"
     CDS             complement(5618..7048)
                     /locus_tag="VNU01_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH31441.1"
                     /translation="MNVADLVGAAAAEHGERTAFRMLDGSSTTWADVDARVDAFAEQV
                     RALGVRPGDRVAIVLPNGVGFAVAFWGVLRAEAVVVPVNPAYTPREMAHILTDSGAVA
                     TVTAEGPARIADAAAEPVDRDTAVICYTSGTTGVPKGALLTHGNFLANLHAFAGLPAL
                     RLTADDVLLGLLPFFHIFGLNVILNAAALHGAEVLVMDRFSPTGSLHAMAEHGVTVAY
                     GAPPVFAAWCAVPAGQAPPLPRLRAAVSGADSLPERVWHAFAERYGVPIQESYGLTET
                     APGLTSNAAAPSPRAGTVGMPLPGVEVRVLAPDGTELGPGQVGEITARGPNVFRGYHG
                     HGAATAEVLDPDGWFRTGDLGEWDADAYLVIKGRLKDLVIVSGFNVYPREVEAVLLEH
                     PGIADAAVVGVPDGRTGERVRAVVVPAPGASLTADAVLDHCRERLARYKLPREVVVAE
                     AIPRRSTGKVERTALRAIETGAAPSG"
     gene            complement(7065..8267)
                     /locus_tag="VNU01_02090"
     CDS             complement(7065..8267)
                     /locus_tag="VNU01_02090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWH31442.1"
                     /translation="MSSASSPLSRRIQQVEESATLAIDAKAKALKAAGEDVIGFGAGE
                     PDFATPAHIVEAAMKACEDPAMHRYTPAAGLPALREAIAQKTLRDSGYEVDASQVVVA
                     NGGKHALYNVFQALVGDGDEVLLPAPYWVSYPEQIKLAGGVPVELATGVATGFRVTVE
                     QLEAARTPRTKALVFVSPSNPTGAVYPPEEVAAIGRWALEHGIWVITDEIYEHLVYGD
                     ARFTSMPVAVPELAARCVVLNGVAKTYAMTGWRVGWSIAPKEVTAGILKLQSHLTSNV
                     SNVAQAAALAAVSGGLEDVAVMRETYDRRRRIAHDKLNAIPGIVCPEPKGAFYVFPSV
                     EGLLGREVAGRVIDSSLTFCEVMLEQAKVALVPGEGFGAPGYVRISYALSDADLTDGL
                     DRLAATLA"
     gene            8422..8937
                     /locus_tag="VNU01_02095"
     CDS             8422..8937
                     /locus_tag="VNU01_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HWH31443.1"
                     /translation="MDQQTAQRVIDLLDQRLVALADLALTLKHVHWNVVGPNFIAVHE
                     MLDDHVAQVRLMVDAVAERIATLGGVPVGTPGHLAKQRTWDDYSVGRADVREHLAALD
                     VVYVGVIRDHRAAIQELDELDLVSQGILLAQAELLEQYQWFVRAHLEDRDGNLSHEGE
                     ATERGAARAAR"
     gene            9084..11498
                     /locus_tag="VNU01_02100"
     CDS             9084..11498
                     /locus_tag="VNU01_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH31444.1"
                     /translation="MLRRPTSRPAILTTLTVAALVAVMPPGPAVGEHRDATVALRAED
                     AVEAAIAWSGHAYPEGATAAALATTGDFADALAGGLVQGTLDAPLLLTPGETLDPRVR
                     AELERLGVTEVRVLGGPAALSEDVVAELADMGLTTTRLSGQTRIETAIAIAEDQAPDA
                     QAVLLARAFAGDGGDPTRAWADSLAAGPLAAGTGRPLLLTATDALPASVVSYLESSSV
                     TTVEVIGGAAAVSDRVVAELEALGMATARLSGPNRAATAVAIAQRRGFADASAAGTVL
                     VLDGQEADAWAAGLAAAGPAVLAQAPMVLASGEVLPPESEAFLSASPDAPPALVCAPL
                     LARRACDLAAGAAGYDALHANATASAAPELVRAELRSVADGVVHVRYWFDEEVRLGSR
                     GAGAFRLAPPDTTAAVAAERAAVAEDAPEAVDAAFTAGHAALHATLALADAGAVQDAQ
                     EVDSPDGGAELGDVRGTPGRTRRPDLVAAVAEAGALVLTFDAPVDTAVGRNIVAVTAD
                     SRAVTGGNATVSDGGRTVRVSVEGLDPEQVRRAALRPGAVFTSAGSNPLSAVAVAGGG
                     LTDGPDLVEVVPRPASDEVRFTFDADLAPFSDGGDRAGFRLRYVDGTEVTAGEATELV
                     DARTVAARFPEGAVNRLVVGAAVAEGAVQTLDAVPAMDDAAGLAQAFAFGESLGPRLI
                     AAELRELEAEGATAAITGDAEVRFVFDGAVTQANSLRFAAVDRDGARVNLSSCSVEPG
                     IAQDTVVCSVPPPPIGEESRMLVAGTVAYDAVRAATAYRTGTEPASGFGRSVSMPGVP
                     WTVPFA"
     gene            complement(11504..12262)
                     /locus_tag="VNU01_02105"
     CDS             complement(11504..12262)
                     /locus_tag="VNU01_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31445.1"
                     /translation="MRTKTTTNPVFFADEALLELSDEARRDALLHDQRSVDLLTWNLF
                     ASLERHRDRAWLADRLRLLGGDKVREPIRISLWTGRTREPRLDPSPRYLALVRERVER
                     SGTTGDALDEAMREFAQPSEVPVRIESPDVLCLVDTYLAGYPVGVGGRDRLLELVDTG
                     LDQAARLGKQLAVAVVYPSGTELGGQVSARVQALRGGLRQELAHRDRVGDVVLRELSW
                     QQLLKVWESEVDWLDLGGQPVKAFLSHCAALGLR"
BASE COUNT         1573 a         4600 c         4743 g         1551 t
ORIGIN      
        1 gaccagcacc gcgcccagca cggcgtcccg cgtgaccggc tcgcccgcca gcagcgcgcc
       61 cagcgcgagc gtcaccacgg ggaacagcac gaacatgtac gagctcgccg acgcggtcca
      121 ccggcgcacc accagcagca ccaggacgaa cagcccggct gacccgaacg tcaccaggta
      181 gcccagggcc agcagcgtgg accggccgga cggcaccgcc cacgcctcgc cggcggccag
      241 cgacaggccg agcagcagga cggcgcccgt ggtcatgccg acggcgttgg tcatggcggg
      301 atggttgccc gacagccgct tgcccaggat gacgctctgc gcgatggcga gcgcggcggc
      361 cgccatggcg gccagcgcgt ccagcggcac ggccgcggcg ccggggccca gcgtcatcca
      421 ggcgataccg gccagcgcca gcagggcccc ggcggcgctg cgcggcgaca gccgctcgga
      481 gccctgggcg gcggccagca gcagcgtcat caacggcacc accgccagca cgatcgtggc
      541 catccccgcg gtgacgcgca ccagcgccca gtacatcagc gcgtagctca gcgcgaacgc
      601 cagcgcgccg tacacggccg tgagcagcag gtcgccgccg cgcggccggc gcagccgcag
      661 cgccaggcag atcgccacga acagcgccgc ggccaggccg aagcgcaggc cggcgcccca
      721 gaacggcggc agctcgcggt tgctgaagcg cacggcgatg aagttggagg cgcccagcgt
      781 cgtggccacc gcgaaggtga ccagcgtcaa gccgtcgggc ccccggtgac ccgctccggt
      841 gtggtccatg cgcccagcat cgcacgcgac cgcccgcttg tggacaactc gcgacgctgg
      901 tggcggccac gaggcacgat gcggctcatg tggtccgagc ccatcgcccg cctgccggaa
      961 cgtgtctgct tcacgctggc cgagaccggt cggctgctgt tctccatcga cgtggccgtg
     1021 ggcctgaccg ctcccgagtc gtccgggggc aggctggcgc gcgccgcgca gcgggtggtg
     1081 acgcgcaggc tctggccgga actggggcgg ctgctggagg accacgacgg cgaggaggcg
     1141 tagcgtgtca cccatgacca ccgccgagcg ccacgtgtcc gtgcccgagg ccgcggaact
     1201 gctgggcatg gacggcggcg agctgtacct gatgatcttc cgcggcggca tccgcgccac
     1261 gcccaccctg gagcaggggg tgctggtgcc cctggacgag gtggagcgcc tggcccggga
     1321 acgccgggcg gacgacgggc gctaggcgcg gcgcaggatc gcgtcgacga agcggtgcac
     1381 cgacttcgcg tcggtgtgga agtacagcag cggctcgatg agctccagct cgatcagctt
     1441 ccagcgctcg ccgatgcgga ccaggtccac ccgcgcgtac aggggctcca cctccgccgc
     1501 ctccaggatg gagcgcgcca ggtccttgac gcgctgcggc ggctggtccg gcgtgatgac
     1561 cccgccgaac tgcggctgca cgcggaagtc gccctcggcg gcctgcttgc gcagcgcgtg
     1621 ggagtagcgc ccgtcgaaga acaccaggga gcgctcgccg ccctgcgtga ccgactcgac
     1681 gaacggctgc accagcacgt cgccctgggc cagcaggccg tccaggtgcg cctggtgctc
     1741 cgcggcctcg cgcaggcgca gccgcacggt gccctgcgag cccgcggaca cggcgggttt
     1801 gaccaccgcc tcgccccagc cccgccggcg caggaccgcg ccgagttcgt gcgacgaccc
     1861 ccgggccagc gtctcggtcg gcaccaccgg cacgccgcgc gcctgcaact cgtccaggta
     1921 gcccttgtgc gcgttccact tcaccacgtt gggcgggttc tccaggcgcg tgaggccgcc
     1981 cacgcggtcc acccatgcgg tgaactcgtc caggcggtgc cagtagtccc acgtggagcg
     2041 gatcaccacc aggtcgaacg acgaccagtc gacgccctcg tcgtcccaca cggcggcggt
     2101 cacgtccgcg ccgcgctcgg tgagcgactt gcgcagcggc tggtcgtcct cgatgaggtc
     2161 gtggtgctcg gcggaggtcg cgagcgcgag cttcatggcg ggggccttac gactggaagc
     2221 gctcgggcag gaacgcccgg ttgggcgtga agctacccga ggcgcgcgcc agcatgcgcc
     2281 cgtcgccgtc ctccacccgg ccgtcggcga tgacgcgcgt cttgccggcg tggacgaccg
     2341 tgccgatcgc gcggtaggcc tggtggggca gcgcggggcg gatgtactcg atgcgcaggt
     2401 ccatcgtcag catgggcacg aagcgctcgc ccagcgaggc gcccgccagg ccggcggcgt
     2461 cgtcgagcac gatcgccagg tagccgccgt gcacgatgcc cgcccggttg cagatgtggg
     2521 gctccgggcg ccactccagc tccacgtggc cgggctcgac ggcgccgatg cgcaggccgg
     2581 cgtgctcctg gtggggcgag cgcggcacgc gcccgtcgct cacggcgcgc aggaagccgg
     2641 cggcgtcggt ctcctgccac agcgcgatga ggcgcgggtc cggttcctgc ccttgcggcg
     2701 ccgcgcccgg ggcggcgggg ccggcgctca cgccatgccc agcgcggcgc gcaggtccgc
     2761 ctccatgacc gccagggacg cctccgacgc cgcggcgcgc aggcgccgct tggtggcggc
     2821 gtacgcgtgg cgcggcaggg ccgcgagcgc ggcggccttg gcctcggcgg ccgccacgac
     2881 atcggccggg tcggccagct cgtcggcgaa gccggcctcc acggcggcct ccggcgagat
     2941 cagggcgccc gacaggatca ggtcctcgta gcggtcggcg cgcacgttcg cgcgggcgat
     3001 cgccaggatc cattccggca tgaccatgcc gatcgcggtc tcgttgagcc cccagcggaa
     3061 ctcgccgcgc gcggcgaccg cgtggtcggc cgccatcgcc aggatggtgc cgcccgccac
     3121 cgaatggccg gtgcaggccg ccaccacggg gcggggctcc agccacacgc ggtgcatggt
     3181 ccgcccgaag cggctcagca gctccagcag cgcctccggc gtggagccct ggagcacctt
     3241 caggttcagg ccgccggaga acatgccgtc gcggccgcgc aggatcaccg cggcggcgtc
     3301 gccgcagtcg tccagcgccg cgtccagctc ggtgaagaag cccaggtcga aggcgttggc
     3361 cttgccgtcg tccatggaca ggtgcgcgaa gtcgccgtcg cgctgcaggg tgatggccac
     3421 gtgaggttcc tgactcgtgc tcgcgaccgc gcgcgggcgc ggacggggtg cggggacggg
     3481 gcggctcaga cgatgccgac gagctcggcc tgggcgatga tgtgctcctt gaccgccttg
     3541 cgcagctcgg cgcgcgtgat gccgggctgc aggtcggtgg tctcgacgtc cagggcgaac
     3601 agccggaaga agatgcggtg cgggccggcg tcggcgtcga cggacgggcc ggtgtagccc
     3661 acctcgtcga actcgttgag cccctgcacg atcgagacct cgtcgtcctc cacgaccgcg
     3721 tcgtccggga tgccctccgg cagcccggtg gtgtccggca ggatgccgta gacgacccag
     3781 tgggtcacca cgccctcctc gccgtcgggg tcgtcgcaga tgagcaccag ctccttggcg
     3841 tcggggggca ccccctccca cgccagaggc ggggacaccc cgtccttgtc cgcggtgtac
     3901 cgcgccggca gcgggtcgtc gtgctcgaac gcgggactgg tcaggcggag cgccgccatc
     3961 gtcgtgctac ctcctgtcgt gatgcgcctc gggcgcaccg ccggtcgcct gcccctccgc
     4021 ctgcggcaga gggtcgaacc aagtcgcggg gcggggcacc gcgcaggcgc cgtcgcgctc
     4081 cagcgcggcg gcggcgcgca ggaccaggtc ctcgcggccg cgggcggcca tgatctgcag
     4141 gccgatgggc atgccgtcgg cgtcggtgcc cacgggcacg gtcacggccg gcagcccgac
     4201 caggttcgcc gggaacgtgt acgcggtgag gcgccgcagc agcggctcgt ccagcaggcc
     4261 gtcgcgccac accgcatccg gcaggcgcgg cacggtcgag gccacggtgg gcgacacgat
     4321 cacgtcgacc tcggcgaaca cggccccgaa cgcgcggtcg atcagcgtgc gcgcctgctg
     4381 cgcgcgcagg tagtccacgg cggggatctc cagggccgcg ccgagcaccg cctggacgtc
     4441 gtcgccgaag cgctccgggt ggtcgcgcag gtcgtcgtag agcgcggcgg cgacctccgc
     4501 gccgatggtg acgtactccg cgacgcggac gaggtccatg tgcgccagcg tgacgggctt
     4561 cgcggccagg ccctcggcga tgggccggca cgcctggtcg atcgcgcggt cgggcgcgcc
     4621 ccaccagtcc cagtccacgc ccaccacgac gccgtccagg ccggcggaca ggtccggcac
     4681 cgccacgtcc gccaggatgg cgtacgcctc ggcgaggtcc gcgctggtcc gggcgatggg
     4741 cccggccacg tccacgctcc accagccgac gttcagctcg ccgcccatcg gcacgcggtc
     4801 gaacgtcggc ttgagtccca gcacgccgca gacggccggc gggatgcgca ggctcccgcc
     4861 cccgtcggtg ccgacggcca gcggcaccat gccggaggcc accgccgcgg ccccgccgct
     4921 ggaggagccg ccgcacggcc gctcgcggtc gtgcgggttg cgcggcgagc cgcccgacgg
     4981 gttcacgccg atgggggaca ggcccagttc cgtcgtgcgg ttcttgccgg cgatgatcgc
     5041 gccggcgcgg cgcagcctgg tgaccagttc cgcctcgggc tgggcgtcgc cggccaggaa
     5101 cgaggtgccg ccacgggtga cgtgcccgcg caccgcgaag aagtccttga cgccgaccag
     5161 cacgccctcc aggggcccgg acggcgcgcc gtcgcgcagg cgccgcgccg acgcggcggc
     5221 ttcggccagc agcgcgtcgg ggtccacgga cacgaacgcg tcgacgtcgc cgccgacggg
     5281 ctgggcggcc aggaagcgct cggcgacctc gacgggcgtg cactcgcccg aggcgtacgc
     5341 ctcgcggtgg gcgccgatgc cggagggggt catgccgtcg gctcctcgcc ctcggccagg
     5401 cggcccggcc gcgtgagctc cgccacgtac ggcgggacgt cgccgagccg ggcgcggcgc
     5461 agcgcggtca cgcccatgag ctgcagcgac acggcgcgca tgccgccgcc cacgaggggc
     5521 gcgcgcgtgg cgcgcgcgac accgcggagg accggaccgg tcatcctgcg aggctgcact
     5581 cggatgctcc cttcctgcga ggtgtggtcg cgaaagccta gccggacggc gcggcgcccg
     5641 tctcgatggc gcgcagcgcc gtgcgctcga ccttgccggt ggaccgccgg gggatcgcct
     5701 cggcgacgac gacttcccgc ggcagcttgt agcgggccag gcgctcacgg cagtggtcca
     5761 gcaccgcgtc ggcggtcagg ctggccccgg gcgccggcac gaccacggcg cggacgcgct
     5821 cgccggtgcg cccgtccggc acgccgacca cggccgcgtc ggcgatcccc gggtgctcca
     5881 gcagcaccgc ctccacctcg cgggggtaga cgttgaagcc gctgacgatc accaggtcct
     5941 tcaggcgccc cttgatcacc aggtaggcgt cggcgtccca ctcgcccagg tcgccggtgc
     6001 ggaaccagcc gtccgggtcc agcacctccg cggtcgccgc gccgtggccg tggtagccgc
     6061 ggaacacgtt gggcccccgc gccgtgatct cgccgacctg cccgggtccc agctccgtgc
     6121 cgtcgggggc gagcacgcgc acctccacgc cgggcagcgg catgccgacg gtgcccgcgc
     6181 gcggggaggg agcggccgcg ttggacgtga gccccggtgc ggtctccgtg aggccgtagc
     6241 tctcctggat gggcacgccg tagcgttcgg cgaacgcgtg ccacacgcgc tccggcaggc
     6301 tgtcggcgcc ggacacggcg gcgcgcaggc gcggcagcgg cggcgcctgg ccggcgggca
     6361 ccgcgcacca ggcggcgaac accggcggcg cgccgtaggc gaccgtcacg ccgtgctcgg
     6421 ccatggcgtg cagcgacccg gtgggggaga agcggtccat gaccagcacc tcggcgccgt
     6481 gcagggccgc ggcgttgagg atcacgttga gcccgaagat gtggaagaac ggcagcaggc
     6541 ccagcaggac gtcgtcggcg gtgagccgca gcgccggcag gccggcgaag gcgtgcaggt
     6601 tggccaggaa gttaccgtgc gtgagcagcg cgcccttggg caccccggtg gtgccgctcg
     6661 tgtagcagat gacggcggtg tcgcggtcga ccggctccgc cgccgcgtcg gcgatgcgcg
     6721 ccggcccttc ggcggtgacg gtggccaccg cgccgctgtc ggtgaggatg tgcgccatct
     6781 cgcgcggcgt gtacgcgggg ttgaccggga ccaccaccgc ttccgcgcgc agcacccccc
     6841 agaacgcgac ggcgaagccg acaccgttgg gcagcacgat ggccacgcgg tcgcccgggc
     6901 ggacgccgag ggcgcggacc tgctcggcga acgcgtcgac gcgcgcgtcc acgtcggccc
     6961 acgtggtgga cgagccgtcg agcatgcgga aggcggtccg ttccccgtgc tcggccgccg
     7021 ccgctcccac cagatccgcg acgttcaccc gtcgagcatg ccagttaggc gagggtggcg
     7081 gcgaggcggt ccaggccgtc ggtgaggtcg gcgtcgctga gcgcgtagga gatgcgcacg
     7141 tagcccggcg cgccgaagcc ctcgccgggc accagtgcca ccttggcctg ctcgagcatc
     7201 acctcgcaga acgtcaggga gctgtcgatg acgcgcccgg cgacctctcg gcccagcagc
     7261 ccctccacgg acgggaacac gtagaaggcg cccttcggct ccgggcacac gatgcccggg
     7321 atggcgttga gcttgtcgtg ggcgatgcgg cggcggcggt cgtaggtctc gcgcatcacg
     7381 gccacgtcct ccagcccgcc ggacacggcc gccagcgcgg cggcctgcgc gacgttggag
     7441 acgttcgacg tcaggtggct ctgcagcttg aggatgccgg cggtgacctc cttgggcgcg
     7501 atcgaccagc ccacccgcca gccggtcatg gcgtaggtct tggccacgcc gttcagcacc
     7561 acgcagcgcg cggccagctc cggcacggcc accggcatcg acgtgaagcg cgcgtcgccg
     7621 tagaccaggt gctcgtagat ctcgtcggtg atgacccaga tgccgtgctc gagcgcccag
     7681 cgcccgatgg ccgccacctc ctccggcggg tacaccgcgc cggtggggtt ggacggggac
     7741 acgaacacca gcgccttggt gcgcggcgtg cgcgccgcct cgagctgctc cacggtgacg
     7801 cggaagccgg tggccacgcc ggtggcaagc tccaccggca cgccgcccgc cagcttgatc
     7861 tgctcggggt agctcaccca gtacggcgcc ggcagcagga cctcgtcgcc gtcgccgacc
     7921 agcgcctgga agacgttgta gagcgcgtgc ttgccgccgt tggcgaccac gacctggctc
     7981 gcgtcgacct cgtagccgct gtcgcgcagc gtcttctgcg cgatcgcctc gcgcagcgcg
     8041 ggaagccccg cggccggcgt gtagcggtgc atcgccgggt cctcgcaggc cttcatggcc
     8101 gcctcgacga tgtgcgcggg ggtggcgaag tccggctcgc cggcaccgaa cccgatgaca
     8161 tcctccccgg cggccttgag cgccttcgcc ttcgcgtcga tcgccagggt ggcggactcc
     8221 tccacctgct ggatccggcg cgacaggggc gaggatgcgc tgctcatggc tcctggtctc
     8281 ttctccgggg cccgtggccc gctgctggtg gccggcgccg ccgcgggcgg ttgccccgcg
     8341 gccgctgcgg ggagggtttc cccgaggccg attccgacac cacgagtgtt gcagatgccg
     8401 aacaacttca ccgtcccggg gatggaccag cagaccgctc agcgcgtgat cgacctgctc
     8461 gaccagcgcc tggtcgctct ggcggacctc gcgctcacgc tcaagcacgt gcactggaac
     8521 gtggtcggcc ccaacttcat cgccgtccac gagatgctcg acgaccacgt ggcccaggtc
     8581 cgcctgatgg tggacgcggt ggcggagcgc atcgccacct tgggcggggt gccggtgggc
     8641 acgccgggcc acctggccaa gcagcgcacg tgggacgact acagcgtcgg cagggccgac
     8701 gtgcgggagc acctggcggc gctggacgtg gtgtacgtgg gggtgatccg cgaccaccgc
     8761 gccgccatcc aggagctgga cgagctggat ctggtcagtc aggggatcct gctggcgcag
     8821 gcggagctgc tggagcagta ccagtggttc gtgcgcgccc acctcgagga tcgcgacggc
     8881 aacctgtcgc acgagggcga ggcgacggag cggggcgcgg cacgggcggc gcgctgaccg
     8941 ccggcctctc cggcccgggg gcggcggccg ccgccctttg cgccagcgtg gtttcgattt
     9001 ccctgagtgg gtacgtcccg ttgcaggccg tcggcgggtg acggcgtcgg cggccaggcg
     9061 gatgagacgg ggaaagggac atcatgctgc gccgtccgac gagcaggcct gccattctga
     9121 cgacgctcac cgtggcggcc ctggtggcgg tcatgccgcc gggccccgcc gtgggtgagc
     9181 accgcgacgc gaccgtggcg ctgcgagcgg aggacgccgt ggaggcggcc atcgcctgga
     9241 gcggccacgc ctacccggaa ggcgcgacgg cggccgcgct ggccaccacg ggcgacttcg
     9301 ccgacgcgct ggcggggggc ctggtgcagg gcaccctgga tgcgcccctg ctgctgacgc
     9361 ccggcgagac gctggacccg cgcgtgcgcg ccgagctgga acggctcggc gtgacggagg
     9421 tccgcgtcct gggcggcccg gccgcgctgt cggaggacgt cgtggcggag ctcgccgaca
     9481 tggggctgac cacgacgcgg ctgtcgggcc agacgcgcat cgagacggcg atcgccatcg
     9541 cggaggacca ggcgcccgac gcgcaggcgg tcctgctggc tcgcgccttc gccggtgacg
     9601 gcggcgaccc cacccgcgcg tgggccgaca gcctggccgc cggaccgctg gccgcgggca
     9661 ccggcaggcc gctgctgctg accgcgaccg acgcgctgcc ggcgtccgtc gtgtcctacc
     9721 tggagtcctc ctccgtgacg acggtggagg tcatcggcgg ggccgccgcc gtgtccgacc
     9781 gggtggtcgc cgaactggag gcgctcggca tggccaccgc ccgcctgtcg gggcccaacc
     9841 gcgccgccac ggccgtggcg atcgcccagc gccgcggctt cgcggacgcg tccgcggccg
     9901 gcaccgtgct ggtcctggat ggccaggagg ccgacgcgtg ggccgccggg ctggcggccg
     9961 ccggtcccgc cgtgctggcg caggcaccga tggtgctggc gtccggggag gtcctgccgc
    10021 cggagtcgga ggcgttcctg tccgcctcgc cggacgcgcc gcccgcgctg gtgtgcgcgc
    10081 cgctgctggc ccggcgcgcc tgcgacctgg ccgccggggc cgccggctac gacgcgctgc
    10141 acgccaacgc cacggcctcg gccgccccgg agctggtgcg ggcggagctg cgctccgtgg
    10201 ccgacggcgt ggtgcacgtg cgctactggt tcgacgagga ggtgcgcctg ggcagccgcg
    10261 gcgcgggcgc gttccgcctg gcgccgcccg acaccacggc ggcggtcgcg gccgagcggg
    10321 ccgccgtggc cgaggacgcg ccggaggcgg tcgacgccgc cttcacggcc ggccatgccg
    10381 cgctgcacgc caccctggcg ctggccgacg ccggcgcggt ccaggacgcc caagaggtcg
    10441 acagtcccga cggcggcgcc gagctcggcg acgtgcgcgg cacccccggc cgcacgcggc
    10501 ggccggacct ggtcgcggct gtggcggagg ccggcgccct ggtgctgacc ttcgacgcgc
    10561 cggtggacac cgccgtgggg cgcaacatcg tggcggtgac cgccgacagc cgcgcggtga
    10621 ccggcggcaa cgcgaccgtc tccgacggtg gccgcaccgt gcgcgtgagc gtggagggcc
    10681 tggacccgga gcaggtccgc agggcggcac tgcgccccgg cgccgtgttc acgtcggcgg
    10741 gctccaaccc cctgtcggcg gtggcggtcg ccggcggcgg cctcaccgac gggccggacc
    10801 tggtggaagt cgtcccgcgg ccggcgtcgg acgaggtccg cttcaccttc gacgccgacc
    10861 tggcgccgtt ctccgacggc ggcgaccgcg ccggcttccg gttgcgctac gtggacggca
    10921 ccgaggtgac cgccggcgag gcgaccgagc tcgtcgacgc ccgcacggtg gcggcgcgct
    10981 tcccggaggg cgccgtcaac cgcctcgtcg tcggcgcggc cgttgccgaa ggggcggtgc
    11041 agacgctgga cgccgtgccg gcgatggacg acgccgcggg gctggcgcag gcgttcgcgt
    11101 tcggggagag cctgggcccg cgcctgatcg ccgcggagct gcgcgagctg gaggcggagg
    11161 gcgcgacggc cgccatcacc ggcgacgcgg aggtgcgctt cgtcttcgac ggcgcggtga
    11221 cgcaggccaa ctcgctgcgc ttcgcggcgg tggaccgcga cggggcgcgc gtgaacctgt
    11281 cgagctgctc ggtggagccg ggcatcgccc aggacacggt ggtgtgctcg gttcccccgc
    11341 cgccgatcgg cgaggagtcg cggatgctgg tggccgggac ggtcgcctac gacgccgtgc
    11401 gcgccgccac cgcgtaccgc acgggcaccg agccggccag cggcttcggc cgcagcgtgt
    11461 cgatgccggg cgtgccgtgg acggtgccgt tcgcctgatc gcgtcagcgc aggccgagcg
    11521 cggcgcagtg ggacaggaac gccttgacgg gctgcccgcc caggtccagc cagtcgacct
    11581 cggactccca caccttcagc agctgctgcc agctgagctc gcgcagcacc acgtcgccga
    11641 cgcggtcgcg gtgggccagc tcctggcgca ggccgccgcg cagcgcctgc acccgcgcgg
    11701 acacctggcc gcccagctcc gtgccggagg ggtagaccac tgccacggcg agctgcttgc
    11761 ccaggcgcgc cgcctggtcc agccccgtgt cgacaagctc cagcaggcgg tcgcggccgc
    11821 cgacgcccac ggggtagccc gccaggtagg tgtcgaccag gcacagcacg tctggtgact
    11881 cgatgcgcac cggcacctcg gacggctggg cgaactcgcg catggcctcg tcgagcgcgt
    11941 cgccggtcgt gccggagcgc tcgacgcgct cgcgcaccag cgccaggtag cgcgggctcg
    12001 ggtccaggcg cggctcgcgc gtccggccgg tccacaaaga gatgcggatc ggctcgcgca
    12061 ccttgtcgcc gccgagcagc cgcaggcggt cggccagcca ggcgcggtcg cggtggcgct
    12121 ccagggaggc gaacaggttc caggtcagca ggtcgacgct gcgctggtcg tggaggaggg
    12181 cgtctcgccg cgcctcgtcg gacaactcca gcagcgcctc gtcggcgaag aacaccgggt
    12241 tggtcgtggt cttggtcctc atggcgcggg atcgtacgcg gtcggcgcgc gggcgggcca
    12301 tcgccgccgc gtccgcgccg ctcgcgggat gctcctatgg atgtcaagtc ggtgatggag
    12361 tggtgaggtc ggccatcagg gcgcggtgga cttctcgggc gatgtagcgc ttgaggcagc
    12421 gcaagatgtc tttgttggtc ttctgttcct tggcgcggcg gtcgcgg
//
LOCUS       DATMKC010000073         7731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_87473, whole genome shotgun sequence.
ACCESSION   DATMKC010000073 DATMKC010000000
VERSION     DATMKC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87473"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VNU01_02110"
     CDS             complement(<1..1340)
                     /locus_tag="VNU01_02110"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWH31446.1"
                     /translation="MSAGAAGLLVFLASAAVLVLEILAGRVLAPFVGVTLETYTAVIG
                     IVLAGIALGASWGGRVADRMDPRRIIGPLLAAGGALALLSVPIVLFLGPPLRGGGAAS
                     VLILTSAGFFLPAVALSAVAPAVVKLRLRDLDQTGHVVGRLSALGTAGALAGVFVTGF
                     VLVALIPTRLIILAVGAALVAGGLLLHWRLKAGPAESPGQDVRAYAALGLVGALLTGV
                     SQAPCDVESAYYCAEVVTDERDPTRRTLILDTLRHSAVDLDDPEYLEFHYMRWFAGVV
                     DARWPGGRPLDALHVGGGGFAMPRWLEATRPGSDSLVLEIDPALVELNRRELGLRDSP
                     GLDVRVGDARNAVRTLPQAAYDVVIGDAFGGLAVPWHLTTLEFAQRLRDLLRPGGVYV
                     VNVIDGPPFDFARAELRTLHEVFAHVAVVAPRDRFDGRTGGNLVLVASDAPLPE"
     gene            1360..2469
                     /locus_tag="VNU01_02115"
     CDS             1360..2469
                     /locus_tag="VNU01_02115"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HWH31447.1"
                     /translation="MIEASDPQPPAGADLRELTAWFVEQQAEASARLGSLLYAALMRH
                     AAQDVRAGGVTWRVLAPHASADRGSALTLRFLAAVHRLVLQGRAPALAASYPSAGGSG
                     DVEGAWQAFHATLEEHEAELVEVVGWSCQTNEVGRAAGLVCGFLRAVARTGVPRLRLL
                     ELGASAGLQLRWDHFRYEWDEHPDTHHWGPPDSPVVLSGHWDPPAELLAQRVEVVERR
                     GCDPDPVDVTTEEGRLRLRQSVWADQPERFARLNGALELAARIPAEVERARAVDWVPA
                     ALAGRVPGTLTVLYHSVVWQYLEPGERDEVRDAIHRAGARATADAPLAWVRSEPENIL
                     RAMRVRLTLWPGGQEELVAKAAAHGLPVRWQTVRA"
     gene            2480..3454
                     /gene="deoC"
                     /locus_tag="VNU01_02120"
     CDS             2480..3454
                     /gene="deoC"
                     /locus_tag="VNU01_02120"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWH31448.1"
                     /translation="MTVVVPSAVDFAAVSDLADAGESDAALRRFLHGLPGVDQVGADA
                     RAATLATRSIKKQAKLQAIDMAIAMTDLTTLEGMDTPGKVRTLCAKARRPDPADPSVP
                     PVAAVCVYGDLVPAAAEALRGSPVRIAAVATAFPSGRASLRVKLEDTREAVAAGATEI
                     DMVIDRGAFLAGRYGQVFDEIRAVKDACGPAHLKVILETGELATYDNVRRASWLALAA
                     GADFIKTSTGKVAPSATLPVALVMLEAVRDVAELTGQYRGVKVAGGVRTTKDAIRYLV
                     LAHEVAGAEWLRADRFRIGASSLLNDLLMQRAKQATGRYWGPDYVTIE"
     gene            3455..4936
                     /locus_tag="VNU01_02125"
     CDS             3455..4936
                     /locus_tag="VNU01_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWH31449.1"
                     /translation="MTETTTPDLVTPTWDYAPAPEARDIVRLSSSYGLFIDGAFVDTL
                     DGGALKTVNPATEEVLAEVAEAGAKDIDRAVAAARRAFTEVWGPMPGRERAKYLYRIA
                     RLIQERAREFAVLESLDNGKPIRESRDVDVPLAAAHFFHHAGWADKLAHAGYGPDPRP
                     LGVAAQVIPWNFPLLMAAWKIAPALATGNTVVLKPAETTPLSALLLAEVVQQAELPPG
                     VVNIVTGAGETGAALVGHPDVDKVAFTGSTEVGKAIQRQIAGTRKKLTLELGGKAANV
                     VFDDAPIDQAVEGIVNGIFFNQGHVCCAGSRLLVQESVETEVLERLKARLATLRLGDP
                     LDKNTDIGAINSAQQLAKITELAASGEQEGAQRWSPESCVLPERGYWFAPTVFTGVAQ
                     SHRIAREEIFGPVLSVLSFRTPQEALDKANNTPFGLSAGVWTEKGSRILWMAQRLRAG
                     VVWANTFNRFDPTSPFGGYKESGFGREGGRQGLAAYLDLEVDA"
     gene            4933..5802
                     /locus_tag="VNU01_02130"
     CDS             4933..5802
                     /locus_tag="VNU01_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWH31450.1"
                     /translation="MSERLQVRRTAKLFVGGAFPRSESGRSYEVTAADGGFLANAALA
                     SRKDARDAVVAARKAFPGWSGATAYNRGQILYRVAELLEGRAAQFIDEVAAAEGVDLD
                     HATAQVDAAIDRWVWYAGWSDKIAMVAGSANPVAGPYFNFSVPEPTGVVAVLAPQGSS
                     LLGMVSVVAPAIVSGNTAVVVACEARPLPAVSLGEVLATSDVPAGVVNVLTGRTAELA
                     PILAGHMDVNAIDLCGAPAEQVPELERLAAENVKRVVRPPREGEPDWTAEPSPRRMLA
                     LTETKTVWHPQGV"
     gene            5931..7034
                     /locus_tag="VNU01_02135"
     CDS             5931..7034
                     /locus_tag="VNU01_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWH31451.1"
                     /translation="MGRAWWKTFIGLLALLLALAACTDGGDGDGDGGEEGEEGSDTEL
                     SEGDGLLAAVRERGTLRCGVNNAVPGFGFLDDEGNYSGFDIDFCRAIAAGVLGDADAV
                     EYVPLTAEQRFTSLQSGDIDVLSRNTTWTASRDGGEGAQFAMTTFYDGQGMMVRADSE
                     FQSVDDMADTDICVLSGTTTELNLATRFSGMNYNPVPFEGNDTLQQAFAEGRCDGWTS
                     DKSQLAGVRSNWPEGPEALRILDETFSKEPLGPAVRQGDTDWFDAVNWSVMATVLAEE
                     LGISSENIDEMMESEDADIRRFLGQPGGEDNSVLDPGLNLEPNFAEQIVRQVGNYGEI
                     YERNVGPDTPLGLERGLNALWTEGGLMYAPPLR"
     gene            7066..>7731
                     /locus_tag="VNU01_02140"
     CDS             7066..>7731
                     /locus_tag="VNU01_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWH31452.1"
                     /translation="MSSDTATVAAATKRPPPWRDVRVLRVAGQVVFLLLVGALLAYLL
                     GNLRDNLRDSGLPTGFGFLDQPAGVDIRDSSFRPSQPVRDAFLVGFLNTLRVAVLGIA
                     IASLIGLLVGIARLSSNWLVRKTAALYVEALRNVPVLVWIVFAYTAVILKLPPITEAA
                     EWLDAVVLSNRGLVVPWYERNLASGALLALALALVGVAAALWVWRTRRSDATGQPHHR
                     VLWA"
BASE COUNT         1073 a         2782 c         2889 g          987 t
ORIGIN      
        1 tcctccggca ggggggcgtc ggaggccacc aggacgaggt tgccgccggt gcgcccgtcg
       61 aagcggtctc gcggcgccac caccgcgacg tgggcgaaga cctcgtgcag cgtccgcagc
      121 tcggcccgcg cgaagtcgaa cggcggcccg tcgatcacgt tgaccacgta cacgccgccg
      181 gggcgcagga ggtcgcgcag ccgctgggcg aactccagcg tggtcagatg ccacggcacc
      241 gccaggccgc cgaaggcgtc gccgatcacc acgtcgtagg ccgcctgagg cagtgtccgg
      301 acggcgttgc gggcgtcgcc gacgcgcacg tccagtcccg gactgtcccg cagccccagc
      361 tcgcgccggt tgagctccac cagcgccggg tcgatctcca ggaccaggct gtcgctgccg
      421 ggccgtgtgg cctccagcca gcgcggcatc gcgaagccgc ccccgccgac gtgcagcgcg
      481 tccagcggcc ggccgccggg ccagcgcgcg tccaccaccc cggcgaacca gcgcatgtag
      541 tggaactcca ggtactccgg gtcgtccagg tccacggcgc tgtggcgcag cgtgtccagg
      601 atcagggtgc ggcgcgtggg gtcccgctcg tcggtgacca cctccgcgca gtagtaggcg
      661 ctctccacgt cgcagggcgc ctgcgacacc ccggtgagca gcgcgccgac gaggcccagc
      721 gcggcgtagg cgcgcacgtc ctggccgggt gactccgccg gtcccgcctt gagccgccag
      781 tgcagcagca gcccgcccgc cacgagtgcc gcgcccaccg ccaggatgat caggcgcgtg
      841 gggatcagcg ccaccagcac gaagccggtg acgaacaccc cggcgagcgc cccggcggtg
      901 cccagggccg acaggcggcc gacgacgtgg ccggtctggt ccaggtcccg cagccgcagc
      961 ttcaccaccg cgggcgcgac cgcggacagc gccacggcgg gcaggaagaa gcccgccgag
     1021 gtgaggatca ggacgctcgc cgcgccgccg ccgcgcagcg gcggccccag gaacagcacg
     1081 atgggcacgg acagcagcgc gagcgcgccg ccggcggcca gcagcgggcc gatgatgcgg
     1141 cgcgggtcca tgcggtccgc cacgcgcccg ccccacgagg cgcccagcgc gatgcccgcc
     1201 agcacgatgc cgatgaccgc ggtgtaggtc tccagggtca cgccgacgaa cggggccagg
     1261 acccgcccgg ccaggatctc caggaccagc accgcggctg aggcgaggaa cacgagcaga
     1321 cctgcggcgc cggcggacat gcgtgcaacg atagccgtca tgatcgaggc ctcggatccc
     1381 cagccgcccg ccggagcgga tctgcgcgag ctgaccgcgt ggttcgtgga gcagcaggcc
     1441 gaggcctccg cgcgcctggg ttcgctgctg tacgcggccc tgatgcgcca tgccgcgcag
     1501 gacgtgcgcg cgggtggcgt gacctggcgc gtgctggccc cgcacgccag cgccgatcgc
     1561 ggcagtgcgc tgacgctgcg gtttctcgcc gcggtgcaca ggctggtgct ccagggccgc
     1621 gcgcccgcgc tggccgcgtc ctacccgtcg gcggggggca gcggcgacgt cgagggcgcg
     1681 tggcaggcgt ttcacgcgac cctggaggag cacgaggccg agctcgtcga ggtggtcggg
     1741 tggtcctgcc agaccaacga ggtggggcgc gccgcgggcc tggtgtgcgg cttcctgcgc
     1801 gccgtcgccc gcacgggcgt gccccggctg cgcctgctgg aactaggggc gagcgccggg
     1861 ctgcagcttc gctgggacca cttccgctac gagtgggacg agcatccgga cacgcaccac
     1921 tggggcccgc cggacagtcc cgtggtcctg tccgggcact gggacccgcc ggcggagctg
     1981 ctcgcgcagc gggtcgaggt ggtcgaacgc cgcggctgcg accccgaccc ggtggacgtc
     2041 accaccgagg agggccgcct gcgcctgcgc cagtcggtgt gggccgacca gccggagcgc
     2101 ttcgcccgcc tcaacggcgc gctcgagctg gccgcgcgca tccccgccga ggtggagcgc
     2161 gcccgcgcgg tggactgggt gccggccgcc cttgcgggtc gcgtccccgg cacgctgacg
     2221 gtgctgtacc actcggtggt gtggcagtac ctggagccgg gcgagcggga cgaggtgcgc
     2281 gacgcgatcc accgggccgg cgcgcgcgcg accgcggacg cgccgctggc ctgggtgcgc
     2341 tcggagccgg agaacatcct gcgcgccatg cgggtccgcc tgacactgtg gcccggtgga
     2401 caggaggagc tggtggccaa ggcggccgcc cacggcctgc cggtgcgctg gcagacggta
     2461 cgcgcatagg cttgtgacga tgactgttgt ggtgccttcc gccgtggact tcgccgccgt
     2521 cagcgacctg gctgacgcgg gcgagtccga cgccgccctg cggcgcttcc tgcacggcct
     2581 gcccggcgtg gaccaggtcg gcgcggacgc gcgcgccgcc acgctggcga cccggtccat
     2641 caagaagcag gccaagctcc aggcgatcga catggccatc gccatgacgg acctgaccac
     2701 gctggaaggc atggacactc ccggcaaggt ccgcacgctg tgcgccaagg cccgccgccc
     2761 ggaccccgcc gaccccagcg tgccgcccgt cgcggcggtg tgcgtgtacg gcgacctcgt
     2821 gcccgcggcg gccgaggcgc tgcgcggcag cccggtgcgg atcgcggccg tcgcgacggc
     2881 gttcccctcc gggcgcgcgt cgctgcgcgt gaagctggag gacacgcgcg aggccgtcgc
     2941 cgccggtgcg accgagatcg acatggtgat cgaccgcggc gcgttcctgg ccggccgcta
     3001 cgggcaggtg ttcgacgaga tccgcgcggt gaaggacgcg tgcggccccg cccacctgaa
     3061 ggtcatcctg gagacgggcg agctcgcgac ctacgacaac gtgcgccgcg cgtcctggct
     3121 ggccctggcc gcgggcgccg acttcatcaa gacctccacc ggcaaggtgg cgcccagcgc
     3181 aaccctgccc gtggccctgg tgatgctgga ggccgtgcgc gacgtcgccg agctcaccgg
     3241 ccagtaccgg ggggtgaagg tcgccggggg cgtgcgcacc accaaggacg cgatccgcta
     3301 cctggtcctg gcccacgagg tggccggagc cgagtggctg cgcgcggacc gcttccgcat
     3361 cggcgcgtcc agcctgctca atgatctgct catgcagcgc gccaagcagg ccaccggccg
     3421 ctactggggc cccgactacg tgacgatcga gtagatgacc gagaccacca ctcccgacct
     3481 ggtcacgccg acgtgggact acgctcccgc gccggaggcc cgcgacatcg tgcgcctgtc
     3541 ctccagctac gggctgttca tcgacggcgc gttcgtcgac acgctggacg gcggagcgct
     3601 caagaccgtg aacccggcca ccgaggaggt cctcgccgag gtcgccgagg ccggcgcgaa
     3661 ggacatcgac cgcgcggtcg ccgcggcccg cagggcgttc accgaggtgt gggggccgat
     3721 gcccgggcgg gagcgcgcca agtacctgta ccggatcgcc cggctgatcc aggagcgcgc
     3781 ccgcgagttc gccgtgctgg agtccctgga caacggcaag ccgatccgcg agtcgcgcga
     3841 cgtggacgtg ccgctggcgg ccgcgcactt cttccaccac gccggctggg cggacaagct
     3901 ggcgcacgcc ggctacgggc cggacccgcg cccgctcggc gtggccgcgc aggtgatccc
     3961 gtggaacttc ccgctgctca tggccgcgtg gaagatcgcg ccggcgctgg ccaccggcaa
     4021 caccgtcgtg ctcaagcccg cggagaccac gccgctgtcg gcgctgctgc tcgccgaggt
     4081 cgtccagcag gcggagctgc cgccgggcgt ggtgaacatc gtcacgggcg cgggcgagac
     4141 cggcgccgcg ctggtcggcc accccgacgt cgacaaggtg gcgttcaccg ggtccacgga
     4201 ggtcggcaag gcgatccagc gccagatcgc cgggacccgc aagaagctca cgctggagct
     4261 gggcggcaag gcggccaacg tcgtgttcga cgacgcgccg atcgaccagg ccgtcgaggg
     4321 catcgtcaac ggcatcttct tcaaccaggg gcacgtgtgc tgcgccggca gccggctgct
     4381 ggtgcaggag tccgtcgaga ccgaggtgct ggagcggctc aaggcccggc tggcgacgct
     4441 gcgcctgggc gacccgctgg acaagaacac cgacatcggc gccatcaact ccgcgcagca
     4501 gctcgccaag atcaccgagc tcgcggccag cggcgagcag gagggtgcgc agcgctggtc
     4561 gccggagagc tgcgtgctgc ccgagcgcgg ctactggttc gcgccgacgg tgttcaccgg
     4621 ggtcgcgcag agccaccgca tcgcgcgcga ggagatcttc ggcccggtgc tctcggtgct
     4681 ctcgttccgc acgccgcagg aggcgctgga caaggcgaac aacaccccgt tcggcctgtc
     4741 cgcgggtgtg tggacggaga agggcagccg gatcctgtgg atggcgcagc gcctgcgcgc
     4801 cggcgtggtg tgggccaaca cgttcaaccg cttcgatccc acctctccct tcggcggcta
     4861 caaggagtcc ggcttcgggc gcgagggcgg ccggcagggt ttggccgcct acctggacct
     4921 cgaggtggac gcatgagcga gcgcctccag gtcaggcgca cggcgaagct gttcgtcggc
     4981 ggggcgttcc cgcgctcgga gtcgggccgc agctacgagg tgacggcggc agacggcggc
     5041 ttcctggcca acgcggcgct ggccagccgc aaggacgcgc gcgacgccgt ggtggcggcg
     5101 cgcaaggcgt tccccgggtg gagcggcgcc acggcctaca accgcgggca gatcctgtac
     5161 cgcgtcgcgg agctgctgga gggccgcgcg gcgcagttca tcgacgaggt ggcggcggcc
     5221 gagggcgtcg acctggacca cgccaccgcg caggtggacg ccgcgatcga ccgctgggtc
     5281 tggtacgccg gctggagcga caagatcgcg atggtcgccg gatcggcgaa ccccgtcgcg
     5341 gggccgtact tcaacttctc ggtgcccgag cccaccggcg tggtggccgt cctggcgccg
     5401 cagggctcca gcctgctggg catggtgagc gtcgtggcgc cggccatcgt cagcggcaac
     5461 accgcggtgg tcgtggcctg cgaggcgcgc ccgctgccgg cggtgtcgct gggcgaggtg
     5521 ctcgcgacca gcgacgtgcc cgccggcgtg gtgaacgtcc tgaccggccg cacggcggag
     5581 ctcgcgccga tcctggcggg ccacatggac gtcaacgcga tcgacctgtg cggcgcgccc
     5641 gccgagcagg tgcccgagct ggagcggctg gccgcggaga acgtcaagcg cgtcgtccga
     5701 ccgccgcgcg agggcgagcc ggactggacc gccgagccgt cccctcgccg catgctcgcg
     5761 ctcacggaga ccaagacggt ctggcacccc cagggcgtgt aggcgcgccg agtgttcgcg
     5821 agcctccgaa cggcttcccg acagaaagcc gtgacggggg cactccggtt ccctaggctc
     5881 accctcgtcc gtacggcgac gcgccctcgg gtgcgagggg aggaccatca gtgggacgag
     5941 catggtggaa gacgttcatc gggctgcttg cgctgctgct tgcgctggcc gcgtgcaccg
     6001 acggcggcga cggcgacggc gacggcggcg aggagggtga agagggcagc gacaccgagc
     6061 tgagcgaggg cgacggcctg ctcgcggccg tgcgcgagcg cggcaccctg cgctgcggcg
     6121 tgaacaacgc ggtgccgggc ttcggtttcc tggacgacga gggcaactac tccggcttcg
     6181 acatcgactt ctgccgcgca atcgccgccg gcgtgctcgg tgacgccgac gcggtcgagt
     6241 acgtgccgct gacagccgag cagcgcttca cgtcgctgca gtccggcgac atcgacgtgc
     6301 tgtcgcgcaa caccacgtgg acggcgtcgc gggacggcgg tgagggcgcg cagttcgcca
     6361 tgaccacgtt ctacgacggc caggggatga tggtccgcgc ggacagcgag ttccagagcg
     6421 tggacgacat ggccgacacc gacatctgcg tgctgtcggg caccacgacg gagctgaacc
     6481 tggccacgcg gttcagcggc atgaactaca acccggtgcc gttcgagggc aacgacacgc
     6541 tccagcaggc cttcgccgag ggccgctgcg acggctggac ctcggacaag tcccagctcg
     6601 ccggcgtgcg ctccaactgg ccggagggcc cggaggcgct gcgcatcctg gacgagacct
     6661 tctccaagga gccgctcggc ccggccgtgc gccagggcga cacggactgg ttcgacgcgg
     6721 tgaactggtc ggtcatggcc accgtgctgg ccgaggagct gggcatcagc tcggagaaca
     6781 tcgacgagat gatggagagc gaggacgcgg acatccgccg cttcctgggc cagccgggcg
     6841 gcgaggacaa cagcgtcctg gacccgggcc tgaacctgga accgaacttc gccgagcaga
     6901 tcgtgcggca ggtcggcaac tacggcgaga tctacgagcg caacgtcggt cccgacacgc
     6961 cgctgggcct ggagcgcggg ttgaacgcgc tgtggaccga gggcggcctg atgtacgcgc
     7021 cgccgctgcg ctaggaggac gggctccgcg aggcgcgtgg gacccatgag cagcgacacc
     7081 gccacggtgg cggccgccac gaagcggccg ccaccgtggc gcgacgtgcg cgtcctgcgc
     7141 gtcgcggggc aggtcgtgtt cctgctgctg gtcggcgccc tgctggccta cctgctgggc
     7201 aacctgcgcg acaacctgcg tgactccggc ctgccgaccg gtttcggctt cctggaccag
     7261 ccggcgggcg tggacatccg cgacagctcc ttccggccgt cccagccggt gcgcgacgcc
     7321 ttcctggtgg gcttcctcaa cacgctgcgc gtcgccgtgc tcggcatcgc gatcgcgtcc
     7381 ctgatcggcc tgctcgtcgg catcgcgcgc ctgtcgtcca actggctggt gcgcaagacc
     7441 gccgccctgt acgtggaggc gctgcgcaac gtccccgtgc tggtgtggat cgtgttcgcg
     7501 tacaccgccg tgatcctgaa gctgccgccc atcacggaag cggccgagtg gctcgacgcg
     7561 gtggtgctgt ccaaccgcgg cctggtggtg ccctggtacg agcgcaacct cgcctccggc
     7621 gcgctgctcg cgctggcgct ggcgctggtc ggcgtcgccg cggccttgtg ggtctggcgc
     7681 acccgccgct ccgacgcgac cgggcagccc caccatcgcg tgctgtgggc c
//
LOCUS       DATMKC010000074         4532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_110578, whole genome shotgun sequence.
ACCESSION   DATMKC010000074 DATMKC010000000
VERSION     DATMKC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110578"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VNU01_02145"
     CDS             complement(<1..605)
                     /locus_tag="VNU01_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HWH31453.1"
                     /translation="MDVLTDIDLVRAVAPRLTPASLRAADAADDGYLGTLDVRFSVFN
                     NWYEIDSFFEGRFLERIAPGAFRKTMKESRDGIKVLYDHGMDFHVGNKVLGPIRELGE
                     DDDAAYGIVPLFDTSYNRDLLPGLREGVYGSSFRFRVVKDEWNDDPGTSRHNPQGIAE
                     RTIKEVRLFEFGPVTFPANPAATAGMRSMTDEFYERLRARNP"
     gene            complement(605..1966)
                     /locus_tag="VNU01_02150"
     CDS             complement(605..1966)
                     /locus_tag="VNU01_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HWH31454.1"
                     /translation="MATLAQRLLSRGKTEQRHDVSISSVDDFFTFLGSSYPFGVNTSW
                     TRGPEVPPPSDYAGYAGMFTSDPVVFAAVAFRLEVFSQGRFQWRELLNGRPQAPFGNE
                     ALRLLERPWFGGRTSDLLAQMLVYGDIAGNAYPLKVDNGRGLSLLRPDWVTLVLGSNL
                     DPEDPGTAEDAELIAIAYSPGGKLGKGSRVYLPGEFAHYAPYPDPMVRYRGMSPLTPI
                     VREVQGDKAATEHKLKFFQHGATPNMVIKHDPSMTAEQVKAFKALFDEEHQGLANAYK
                     TLHLGGGADATVVGANLQQIDFKSTQGKSETRILMALGVHPTLVGASEGMQGSSLNAG
                     NYSQTRRAFSDVKLQHLWGEACGALEVLLPRPSDSAHLVIDQRDIPFLQDDQKDKAEI
                     QNHQANAMRTLVDGGFEPDSVRDAVLADDYSLLKHTGKLSVQLQEPGTDPDAAPSTEG
                     DDA"
     gene            complement(1970..2107)
                     /locus_tag="VNU01_02155"
     CDS             complement(1970..2107)
                     /locus_tag="VNU01_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31455.1"
                     /translation="MNAVAVVLTALGLGLSAGAFGLRPGLFIGGLALAGLTMFVLDVE
                     G"
     gene            complement(2104..3411)
                     /locus_tag="VNU01_02160"
     CDS             complement(2104..3411)
                     /locus_tag="VNU01_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31456.1"
                     /translation="MPWQRYVADVAGEYDALTRIPFYRDIGTTVPRQQGKTTLSFAEM
                     VDRGLNWGSPQRSVFTAQTGKDARDKFLDEIFPNLRASKLMPLVKQILEGMGNEGIRW
                     KTGSILRLASTSAGTGHGKTIGQAMLDEIWHDVDDRREQGLRPAMLTVADAQLWWWST
                     AGTAASTIYNRKVKTGRGAVLADTGSGTAFFDWGAGENWDPDDWDSYFGFMPALCPNP
                     PCRCGDGDGGWRHTVTLDVLKAERAAMSDGEYRRAYGNIPTAGADVVIPLELWRELCD
                     PDVKPEGALRFGLAVSPERESAAIVSCADGVLELVDHRAGTGWVVDRCNALTAKHGGK
                     VALDKGGPAGVFADEIRSCDAMQGGDVIRAHGAFYDAVIQKRVKFRTDPALDAAVEGA
                     VKKIVGDNWVWSRRASMNDVTPLEAATLAYAPAEARESFFLFS"
     gene            complement(3479..3820)
                     /locus_tag="VNU01_02165"
     CDS             complement(3479..3820)
                     /locus_tag="VNU01_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31457.1"
                     /translation="MDCPDCDFVAKNGNGLARHRQAKHKEQRAEPGPNERAIAITLRA
                     LGEAGRLEAVDEARVTALRTMAVALDLNPFNSQMFREYREALEGLMVDDSDSGGLEEL
                     IAAMRSPVRDT"
     gene            complement(3824..4102)
                     /locus_tag="VNU01_02170"
     CDS             complement(3824..4102)
                     /locus_tag="VNU01_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWH31458.1"
                     /translation="MGRRGEFSHSSPAWRAVRLAALERDGYLCQIRGPHCRVKATEGD
                     HIVPLALGGAWFDLDNVRASCKPCNAGRMMREAVGGGQAAVRRPSREW"
     gene            complement(4236..>4532)
                     /locus_tag="VNU01_02175"
     CDS             complement(4236..>4532)
                     /locus_tag="VNU01_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31459.1"
                     /translation="PPYRMQVPWSREADVTTLHASTMTPEQHTQIVPWLEAHGITASE
                     CYQVTYTADTLTAYRYALDAEGNHIVEGDDLAVAEQVTVPIQTPPPASLATTGR"
BASE COUNT          660 a         1641 c         1495 g          736 t
ORIGIN      
        1 gggttgcggg cgcgcaggcg ctcgtagaac tcgtcggtca tggaccgcat gcccgccgtg
       61 gcggcggggt tggccgggaa cgtcaccggg ccgaactcga acaggcggac ctccttgatg
      121 gtccgctccg cgatcccctg cgggttgtgc cgggacgtgc ccgggtcgtc gttccactcg
      181 tccttgacga cgcggaaacg gaagctcgac ccgtacacgc cctcgcgcag gcccggcagc
      241 aggtcacggt tgtaggaggt gtcgaacagc gggacgatgc cgtacgccgc gtcgtcgtcc
      301 tcgcccaact cgcggatcgg acccaggacc ttgttgccga cgtggaagtc catgccgtgg
      361 tcgtagagga ccttgatccc gtcgcgggac tccttcatcg tcttgcggaa cgcgcccggc
      421 gcgatgcgct ccaggaagcg gccctcgaag aacgagtcga tctcgtacca gttgttgaag
      481 acggagaagc gcacgtccag cgtccccagg tagccgtcgt cggcggcgtc ggccgcgcgc
      541 aggctcgccg gcgtgaggcg cggcgcgacc gcgcgcacaa ggtcgatgtc ggtcagaacg
      601 tccatcaggc gtcgtctcct tcggtggacg gtgcggcgtc gggatcggtg cccggctcct
      661 ggagttgcac cgagagcttc ccggtgtgct tgagcagcga gtagtcgtcc gccagaaccg
      721 cgtcgcgtac cgagtccggc tcgaagccgc cgtccaccag cgtccgcatc gcgttcgcct
      781 ggtggttctg gatctcggcc ttgtccttct ggtcgtcctg caggaagggg atgtcgcgct
      841 ggtcgatgac gagatgcgcc gagtccgacg ggcgcggcag cagcacttcc agcgcgccgc
      901 acgcctcgcc ccacaggtgc tggagcttca cgtcggagaa cgcccggcgg gtctgggagt
      961 agttgcccgc gttcaggctc gacccctgca tgccctccga ggcgccgacc agcgtgggat
     1021 gcacgcctag cgccatgagg atccgcgtct cggacttgcc ctgcgtggac ttgaagtcga
     1081 tctgctggag gttcgcgccg accaccgtgg cgtccgcacc accgcccagg tgcagcgtct
     1141 tgtaggcgtt ggcgagcccc tggtgctcct catcgaacag cgccttgaac gccttgacct
     1201 gctcggccgt catggacggg tcgtgcttga tgaccatgtt cggcgtcgcg ccgtgctgga
     1261 agaacttgag cttgtgctcc gtcgccgcct tgtccccctg cacctcccgc acgatcggcg
     1321 tcaacgggct catgccgcgg tagcgcacca tcgggtccgg gtagggcgca tagtgcgcga
     1381 actcgcccgg caagtagacg cgtgagccct tgccgagctt gccgcccggc gagtaggcga
     1441 tcgcgatgag ctcggcgtcc tcagccgtgc ccgggtcctc cgggtccagg ttcgagccga
     1501 gaaccagcgt cacccagtcc gggcgcagca gcgacagtcc ccggccgttg tccaccttca
     1561 acgggtaggc gttgcccgcg atgtcgccgt acaccagcat ctgcgccaga aggtccgacg
     1621 tgcggccccc gaaccagggg cgctccagca gccgcagcgc ctcgttgccg aacggcgcct
     1681 gcgggcggcc gttcagcagc tcccgccact ggaaccggcc ctgggagaac acctccagcc
     1741 ggaacgccac cgccgcgaac accaccgggt ccgacgtgaa catccccgcg tacccggcgt
     1801 agtccgacgg cggcggcacc tccggccctc gcgtccacga ggtgttcaca ccgaacgggt
     1861 aggaggaccc caggaacgtg aagaagtcgt ccacgctgct gatcgacacg tcatggcgct
     1921 gctcggtctt gcccctggag aggagccgct gcgcaagtgt cgccaccggc tacccctcca
     1981 cgtcgagcac gaacatggtc agcccggcca gggccagccc gccgatgaac aggccgggac
     2041 gcagcccgaa tgcgccggcc gacagtccca ggcccagcgc ggtgagcacg acagcgaccg
     2101 cgttcacgag aacaggaaga acgactcgcg ggcctcggcc ggggcgtagg cgagcgtggc
     2161 cgcctccagg ggtgtcacgt cgttcatgga tgccctccgt gaccagaccc agttgtcacc
     2221 gacgatcttc ttcaccgcgc cctccaccgc cgcgtccaag gcggggtcgg tgcggaactt
     2281 cacgcgcttc tgaatcacgg cgtcgtagaa ggcgccgtgc gcgcggatca cgtcgccgcc
     2341 ctgcatcgcg tcgcaggacc gaatctcgtc cgcgaacacc ccggcggggc cgcccttgtc
     2401 cagcgccacc ttgccgccgt gcttggcggt cagcgcgttg cagcggtcca ccacccagcc
     2461 ggtgccggcc cggtgatcca ccagttccag caccccatcg gcgcaggaca cgatcgcggc
     2521 cgactcgcgt tcgggcgaca cggccagccc gaaccgcagc gcgccctccg gcttgacatc
     2581 cgggtcgcac agttcgcgcc acaactccag tgggatcacg acatcggccc ccgcggtcgg
     2641 gatgttgccg taggcccgcc ggtactcgcc gtcgctcatc gccgcacgct cggccttgag
     2701 cacgtccagc gtcaccgtgt ggcgccagcc gccgtcgccg tcaccgcacc tgcagggtgg
     2761 gttcgggcag agcgccggca tgaagccgaa gtaggagtcc cagtcgtcgg gatcccagtt
     2821 ctcccccgcg ccccagtcga agaacgccgt tcccgagccg gtgtcggcca ggacggcccc
     2881 acggcccgtc ttgaccttgc ggttgtagat cgtggaagcg gcggtgcccg cagtggacca
     2941 ccaccacagt tgcgcgtccg cgacggtcag catcgccggg cgcagaccct gctcgcggcg
     3001 gtcgtccacg tcgtgccaga tctcgtccag catcgcctgg ccgatggtct tgccgtggcc
     3061 cgtgccggcc gaggtggacg cgaggcgcag gatcgagccg gtcttccacc ggatgccctc
     3121 gttgcccatc ccttcgagga tctgcttcac cagcggcatc agcttcgagg cccgcaggtt
     3181 cggaaagatc tcatccagga acttgtcgcg cgcgtccttg ccggtctgcg cggtgaacac
     3241 cgaccgctgg gggctgcccc agttcagccc ccggtccacc atctccgcga aggagagcgt
     3301 cgtcttgccc tgctggcgcg gaaccgtcgt gccgatgtcc cggtagaacg ggatccgcgt
     3361 gagtgcgtcg tactcgccgg ccacgtccgc gacgtagcgc tgccacggca tgagcggctg
     3421 gcccagcagc tcagcgacct tcgcgaccct gccccccgtg gtcgggcgct ccggccgcct
     3481 acgtgtcgcg taccggggag cgcatagccg cgatgagctc ttcaaggccg ccgctgtccg
     3541 agtcatcgac catcagcccc tccagcgcct ccctgtactc gcggaacatc tgcgagttga
     3601 aggggttcaa gtcgagggcc accgccatgg tcctgagcgc cgtcaccctg gcctcatcca
     3661 cggcctccag ccgcccggcc tcgcccagcg ccctcagggt gatcgcgatc gcgcgctcgt
     3721 tcggccccgg ctcggcgcgc tgctccttgt gcttcgcctg ccggtgccgg gccagcccgt
     3781 tgccgttctt cgccacgaag tcacagtcgg gacagtccat cggctaccac tcccgactcg
     3841 gccggcgcac cgccgcctgt ccaccgccca cggcctcacg catcatccgg cccgcgttgc
     3901 acggcttaca cgacgcgcgc acgttgtcca ggtcgaacca cgcaccgccc agggccagcg
     3961 gcacgatgtg atccccctcc gtcgccttca cccgacagtg cggcccgcgg atctgacaga
     4021 ggtacccgtc gcgctccaaa gcggccagtc gcaccgcccg ccacgccgga ctcgaatgac
     4081 tgaactctcc ccgtcgcccc atggccgacc tcgaaacatt cgatccgtgt aaaaaagagg
     4141 cgcggagctt ccgcgtgccc ggcgcggcgg tgcgtgtgga ggccgccccc ctacccctgc
     4201 cggtccctgc gggcttctgc cgtcctgtcg cgtggtcagc gtccagtcgt ggcgagcgag
     4261 gcgggaggcg gggtctggat cggtacggtg acctgctctg ccactgctag gtcgtcgcct
     4321 tccacgatgt ggttgccttc ggcgtcgagc gcgtagcggt aggcggtcag ggtgtctgcc
     4381 gtgtaggtga cctggtagca ctcggatgcg gtgatgccgt gcgcctccag ccatgggacg
     4441 atctgagtgt gctgctcggg tgtcatggtg gatgcgtgca gtgtggtcac gtcagcctca
     4501 cgggaccagg gcacctgcat gcggtatgga gg
//
LOCUS       DATMKC010000075         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_281230, whole genome shotgun sequence.
ACCESSION   DATMKC010000075 DATMKC010000000
VERSION     DATMKC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281230"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VNU01_02180"
     CDS             <1..965
                     /locus_tag="VNU01_02180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH31460.1"
                     /translation="RDPWERMAYRGEMHQLVSTPAGPPLVASLIRSLAARVREQARAG
                     APDVLHVHWWVPGAIAARLAAVDVPTVVTVHGTDVALIEARPRLAALARWALGGADRV
                     EAVSSDLAERLERAVGIRADAVNPMPLDPSWLRPAAVVPRAPDGVARVLAVGRMVPEK
                     GFADLIAAVARLKQPVVLELIGDGPEFDRLLALADELRVALELPGRVAPAGLRAAYAR
                     ADVVAQPSHREGLGLVAAEAVLAGTPVVAADSGGVRDVLPEDRLVPAGDVGALAERLG
                     RALANLPAEREATAALAAGIAQRLSPQASAERTLAGYEALMERP"
     gene            complement(999..1994)
                     /locus_tag="VNU01_02185"
     CDS             complement(999..1994)
                     /locus_tag="VNU01_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWH31461.1"
                     /translation="MRAVILAGGQGTRLRPLTDSRPKPLVPFMGEPFALGLLRRLADI
                     GVTRADFLVGDDAAPFAPLHAAGERAGVEVAVRTEESPLDTAGAVRRLFAEGAGEQPV
                     LVCNGDILTDLDYAGLVRAHGDSGADATLALTRVADTSSFGVVVCRADGRVERFVEKP
                     PPGTLDADTVNAGTYVLAPDVFDAFPGDGPLSFEREVFPGLLEAGRTLLGVTSDAYWQ
                     DLGTPRRYLDGHRAVLDGRCDWPGDPLLERRGGEVAVHRDAKVDPSAKLGPGTVILAH
                     CTIGAGARLTDSVLLEGALVGDRAMVRGSILCEVASVEPAAALDAMVLPQGASFG"
     gene            complement(2004..>3195)
                     /locus_tag="VNU01_02190"
     CDS             complement(2004..>3195)
                     /locus_tag="VNU01_02190"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWH31462.1"
                     /translation="RHLVGPGGPLRAAVERDQLSSVILWGPPGTGKTSLAHVIAATTQ
                     AAFTELSAVTAGVKDVRAVVEQARDRRAASDRRTVLFVDEIHRFNKSQQDALLPGVED
                     GSVVLIGATTENPYFEVNAPLLSRSLLYRLEPLDGDDVRALLERAVADERGLPGLAVA
                     PEALDALVAAADGDARVALTGLEAAAATRGADLVTAEMVKAALGRPHLRYDRDDSHYD
                     QVSAFIKAMRGSDPDAAVYWLVRMLAGGEDPRFVARRMVILAGEDVGLADPRALQVAV
                     AAFQALDVVGLPEARYALAEAAAYLALAPKSKSVTTALARADEAVRRLGNAPVPAHLR
                     DAHYKGAAKLGHGEGYRYPHDDPRGWVEQRYLPEGLEPGAIFSPGEHGEEAILWERLR
                     RRRE"
BASE COUNT          378 a         1229 c         1209 g          379 t
ORIGIN      
        1 gccgtgaccc gtgggagcgc atggcctacc gcggggagat gcaccagctg gtgagcacgc
       61 ccgccggccc gccgctggtc gcgtccctga tccggtcgct ggcggcgcgc gtgcgcgagc
      121 aggcgcgcgc cggtgcgccc gacgtcctgc acgtgcactg gtgggtgccc ggcgcgatcg
      181 cggcgcggct ggcggccgtc gacgtgccga ccgtggtgac ggtgcacggc accgacgtgg
      241 cgctgatcga ggctcgcccg cggctggcgg cgctggcgcg ctgggcgctg ggtggcgccg
      301 accgcgtgga ggcggtgtcg tccgacctgg ccgagcgcct ggagcgcgcc gtcggcatcc
      361 gcgcggacgc ggtcaacccg atgccgctcg acccgtcgtg gctgcgcccg gcggccgtgg
      421 tgccgcgcgc gcccgacggt gtcgcgcggg tgctcgcggt ggggcgcatg gtcccggaga
      481 agggcttcgc cgacctgatc gcggcggtgg cgcgcctgaa gcagccggtg gtactggagc
      541 tgatcggcga cggcccggaa ttcgaccggc tcctggcgct ggccgacgaa ctgcgcgtgg
      601 cgctggagct gccggggcgg gtggcgccgg cggggctgcg cgccgcctac gcgcgcgccg
      661 acgtggtcgc gcaaccgtcg catcgggagg gcctcggcct ggtggcggcc gaggcggtgc
      721 tggcgggcac gccggtggtg gccgccgact ccggcggtgt gcgcgacgtg ctgcccgagg
      781 accgcctggt ccccgcgggc gatgtcggcg cgctggcgga gcggctggga cgcgcgctgg
      841 cgaacctgcc cgcggaacgc gaggcgacgg cggccctggc cgccgggatc gcccagcgcc
      901 tgtcaccgca ggcctcggcc gagcgcaccc tcgccggcta cgaggcgctc atggagcgcc
      961 cctagccgaa gttgacgccc agcctcaacc cttcggcgtc agccgaagct ggcgccttgg
     1021 ggcagcacca tcgcgtcgag ggcggccgcg ggctccacgg acgccacctc gcacaggatg
     1081 gagccgcgca ccatggcgcg gtcccccacc agcgccccct ccagcagcac cgaatcggtc
     1141 aggcgcgcac cggcaccgat cgtgcagtgc gccaggatca ccgtgccggg gccgagcttg
     1201 gcgctcgggt ccaccttggc gtcacggtgc accgcaacct cgccgccgcg ccgctccagc
     1261 agcgggtcgc ccggccagtc gcagcgcccg tcgagcacgg cgcggtggcc gtccaggtag
     1321 cgccgcggcg tgccgaggtc ctgccagtac gcatcgctcg tcacaccgag cagcgtgcgg
     1381 ccggcctcca gcagcccggg gaagacctcg cgctcgaacg acagcgggcc gtcgcccgga
     1441 aaggcgtcga agacgtccgg cgccagcacg tacgtcccgg cgttcaccgt gtcggcgtcg
     1501 agcgtgccgg gcggcggctt ctcgacgaag cgctccaccc gcccgtccgc gcggcacacc
     1561 acgacaccga agctcgacgt gtcggcgacg cgggtcagcg cgagcgtggc gtcggcgccg
     1621 ctgtcgccgt gagcgcgcac cagcccggcg tagtccaggt cggtgaggat gtcgccgttg
     1681 cagaccagca cgggctgctc gccggcgccc tcggcgaaga gccggcgaac cgcgccggcc
     1741 gtgtccagcg gcgactcctc ggtgcgcacc gcgacctcga cgccggcccg ctccccggcg
     1801 gcgtgcagcg gcgcgaaggg cgcggcgtcg tcacccacca ggaagtcagc gcgcgtcacg
     1861 ccgatgtcgg cgaggcggcg cagcagcccg agcgcgaacg gctcgcccat gaagggcacc
     1921 agcggcttgg gacggctgtc ggtgagggga cgcaggcggg tgccctggcc gcccgcgagg
     1981 atcacggcac gcatgccgcc aggctactcg cggcggcggc gcaggcgctc ccacaggatc
     2041 gcctcctcgc cgtgctcgcc gggtgagaag atcgcgccgg gctccaggcc ctccggcagg
     2101 tagcgctgct cgacccagcc gcgcgggtcg tcgtgcgggt agcggtagcc ctcgccgtga
     2161 ccgagcttcg ccgcgccctt gtagtgcgcg tcgcgcaggt gcgcgggcac cggcgcgttg
     2221 cccaggcgcc gcacggcctc gtcggcgcgt gccagcgccg tggtgacgct tttggacttc
     2281 ggcgccagcg ccaggtaggc cgccgcctcg gccagcgcgt agcgcgcctc cggcaggccc
     2341 accacgtcca gcgcctggaa cgccgcgacc gccacctgca acgcgcgcgg gtcggccagg
     2401 cccacgtcct cgcccgccag gatcaccatc cgccgcgcca cgaagcgggg atcctctccc
     2461 ccggccagca tgcgcaccag ccagtacacc gcggcgtccg ggtccgagcc ccgcatggcc
     2521 ttgatgaacg cgctgacctg gtcgtagtgg ctgtcgtcgc ggtcgtagcg caggtgcggc
     2581 cgccccagcg ccgccttcac catctccgcc gtgaccaggt ccgccccgcg cgtcgccgcg
     2641 gcggcctcca gcccggtcag cgccacgcgc gcgtcaccgt ccgcggcggc caccagcgcg
     2701 tccagcgcct cgggcgccac cgccaggccg ggcaggccgc gctcgtcggc gaccgcgcgc
     2761 tccagcagcg cgcgcacgtc gtcgccgtcc agcggctcca gccgatacag caggctgcgc
     2821 gacagcagcg gcgcgttcac ctcgaagtac gggttctccg tggtggcgcc gatcagcacc
     2881 acggagccgt cctcgacgcc gggcagcagc gcgtcctgct gggacttgtt gaagcggtgg
     2941 atctcgtcca cgaacagcac ggtgcgccgg tccgaggcgg cgcggcggtc ccgcgcctgc
     3001 tccaccaccg cccgcacgtc cttcaccccg gcggtcacgg cggacagctc ggtgaacgcc
     3061 gcctgcgtgg tggcggcgat cacgtgggcg agcgaggtct tgccggtgcc gggcggtccc
     3121 cacaggatca cgctggacag ctggtcgcgc tccacggcgg cgcgcagcgg gccgccgggg
     3181 ccgacgagat gacgc
//
LOCUS       DATMKC010000076         3543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_67283, whole genome shotgun sequence.
ACCESSION   DATMKC010000076 DATMKC010000000
VERSION     DATMKC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67283"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VNU01_02195"
     CDS             complement(<1..1200)
                     /locus_tag="VNU01_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH31463.1"
                     /translation="MAKPVVLAVDDEPRVLSAVEADLRSHYGRDYHVVGAGSGEEALE
                     ALRTLALRGRPVALVLADQRMPGMTGVELLREARALAPEAKRALLTAYADTEAAIRAI
                     NEVALDHYLLKPWDPPEERLYPVVDDLLDEWWASYRPGAQGIRVVGHRWSREAHELKD
                     FLARNQIPYTWLDVERDPEAARLLTSAGADTAAGPVVVLEDGQALVAPSPAEVADRVG
                     LHTRAALPFYDLVVVGGGPAGLAAGVYGSSEGLRTLVVERLAPGGQAGLSTLIENYLG
                     FPKGLSGADLARRANAQALRLGAELLASQEVAGLDARDGFHVLRLGDGTEVAANGCIV
                     ATGVAYRMLDVPGAEGLTGSGVYYGASREEAQAHADEDVFVLGGGNSAGQAAAYLSAH
                     CASVTILV"
     gene            1423..>3543
                     /locus_tag="VNU01_02200"
     CDS             1423..>3543
                     /locus_tag="VNU01_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH31464.1"
                     /translation="MRAAAVPHRRRLAAALLVSALAATGLPRPAAAAGAPELSVSSVP
                     TGEDFAAVAYGDPFDFANPQDLRLLPDRTTPALHSPRMESGEYRFDARGGASLTTFVF
                     PGYPGALSMERDGGALPIDADRYTHLAMRIFSPSQRAMSFSFDRCLSAEFANQFCRTS
                     TDNVILEPGWNTVVRDLRALTTGRQWPWAGKVYAFRMMNDVADGYRVGYLRLQTAPET
                     VEVTWDRSAGTDLYWDADTSLANNTPDAPGWGRLGGSGTATFPAGAMEAGTYAFYART
                     GSGTPSAYSAGLRVAPRPAPVVLDPDLAGGEDYATVANGKPWDFSTPADVIAQGGFTG
                     GTFGPGGLQGTNSGNDPFFYLRMNQPLDASRYHRLTVHATYDGAFNLADTAGGGTHGR
                     LLWTRADRDQTHVIQGNKLIESRELVHYPGQSSFTVDLKTPPDPALVMETDRPERDGW
                     STGSPVTHLRYDPNEDRGARRWRIARIELRATDATSANAFDIRWRDDSGATGVKVTLS
                     YRPESGGAETAIAGGIDQQPGVNTYRWDTAGVAPGRYVVRVTGDDGVSRGSGTSTGPV
                     DVRASLRTAGSNRIATGVALSRLAYPGGAGTAVLARADGFADALAAAPLAAAADGPLL
                     LNAGDRLDPAVAAELDRLKAATVFLLGGESAQSSAVEAALEASTAEDCADSPPSRKTV
                     AAFRRSSSAATAGSSRSPAFSRSGP"
BASE COUNT          452 a         1428 c         1245 g          418 t
ORIGIN      
        1 caccaggatc gtcaccgagg cgcagtgggc ggacaggtag gcggccgcct gccccgccga
       61 gttgcccccg cccagcacga acacgtcctc gtccgcgtgc gcctgcgcct cctcgcggct
      121 ggcgccgtag tacacgcccg agccggtcag cccctccgcg cccggcacgt cgagcatgcg
      181 gtaggccacg cccgtcgcga cgatgcagcc gttcgcggcg acctcggtgc cgtcaccgag
      241 ccgcaggacg tggaacccgt cgcgcgcgtc gaggccggcg acttcctgcg aggcgagcag
      301 ctccgcgccc aggcggagcg cctgcgcgtt ggctctgcgg gcgaggtccg cgcccgacaa
      361 ccccttggga aagcccaggt agttctcgat cagcgtgctc aggcccgctt ggcctcccgg
      421 cgccagccgt tccacgacga gcgtgcgcag cccctccgac gagccgtaca ccccggccgc
      481 gagcccggcc ggaccgcccc cgaccaccac caggtcgtag aacggcagcg cggcccgggt
      541 gtgcagtccc acgcggtcgg cgacctccgc gggcgacggc gccaccagcg cctgcccgtc
      601 ctccaggacc accaccgggc cggcggcggt gtccgcgccc gcgctggtga gcaggcgcgc
      661 cgcctccggg tcgcgctcca cgtcgagcca ggtgtagggg atctggttgc gcgccaggaa
      721 gtccttcagc tcgtgggcct cgcgcgacca gcggtgcccg acgacgcgga tcccctgggc
      781 tcccgggcgg taggaggccc accactcgtc gagcaggtcg tcgacgaccg ggtacaggcg
      841 ctcctcgggg ggatcccacg gcttgagcag gtagtggtcg agcgcgacct cgttgatcgc
      901 gcggatcgcc gcctccgtgt cggcgtacgc ggtcagcagc gcccgcttgg cctcgggcgc
      961 gagcgcgcgc gcctcgcgca gcagctccac gccggtcatg ccgggcatgc gctggtcggc
     1021 gagcaccagg gcgacggggc ggccgcgcag cgccagcgtg cgcagcgcct ccagcgcctc
     1081 ctcgccggag ccggcgccga ccacgtgata gtcgcggccg tagtggctgc gcaggtcggc
     1141 ctccaccgcg ctgagcacgc gtggctcgtc gtccacggcc aggaccaccg gcttggccaa
     1201 tgtccctccc tccgccgatc cccgtcgagt ttcccgccag ggcaacctac cccgcgaccc
     1261 ggggcagcgg aagccgcccg ggcgcgacga ccgcccgcgc cgggccgggc cgccacatcc
     1321 tgacgggtcc ggggttgtgt gcggcggcgc ggctgccgac acagaaggtg accggcctcc
     1381 cctggccggt cgcgaagcgt gcgacgagga gcaggccccc tgatgcgtgc agccgctgtc
     1441 ccccaccgcc ggcgcctggc cgccgccctc ctggtgtcgg cgctggccgc cacgggcctg
     1501 ccgcgcccgg ccgccgccgc gggcgcgccg gagctgtccg tgtcctccgt gcccaccggg
     1561 gaggacttcg ccgcagtcgc ctacggcgac ccgttcgact tcgccaaccc ccaggacctg
     1621 cgcctgctgc cggaccgcac caccccggcg ctgcacagcc cgcgcatgga gtccggcgag
     1681 taccgcttcg acgcgcgcgg cggcgcgtcc ctgaccacgt tcgtcttccc cggctacccg
     1741 ggcgcgctgt ccatggagcg cgacggcggc gccctgccga tcgacgccga ccgctacacc
     1801 cacctcgcga tgcggatctt ctcccccagc cagcgcgcga tgtcgttctc gttcgaccgc
     1861 tgcctgtcgg cggagttcgc caaccagttc tgccgcacgt ccacggacaa cgtcatcctg
     1921 gagcccggct ggaacaccgt ggtgcgcgac ctgcgcgccc tcaccacggg caggcagtgg
     1981 ccgtgggcgg gcaaggtcta cgcgttccgc atgatgaacg acgtggccga cggctaccgc
     2041 gtcggctacc tgcgcctgca gaccgccccg gagaccgtgg aggtgacctg ggaccgctcc
     2101 gcgggcaccg acctgtactg ggacgccgac acgagcctgg ccaacaacac gccggatgcg
     2161 ccgggctggg gcaggctcgg cgggagcggc acggcgacgt tcccggcggg cgccatggag
     2221 gccggcacgt acgcgttcta cgcgcgcacc ggctcgggca ccccgtccgc ctacagcgcc
     2281 gggctgcggg tcgccccccg cccggcgccc gtggtgctgg acccggacct cgccggcggc
     2341 gaggactacg ccaccgtggc caacggcaag ccgtgggact tctcgacacc cgccgacgtg
     2401 atcgcccagg gcgggttcac gggcggcacg ttcggccccg gcggcctgca gggcaccaac
     2461 agcggcaacg acccgttctt ctacctgcgg atgaaccagc cgctggacgc ctcgcggtac
     2521 caccgcctca ccgtgcacgc cacctacgac ggcgccttca acctcgccga cacggccggc
     2581 ggcggcacgc acgggcgcct gctgtggacg cgcgccgacc gcgaccagac gcacgtgatc
     2641 cagggcaaca agctgatcga gtcgcgcgag ctggtgcact acccgggcca gagctcgttc
     2701 acggtcgacc tgaagacgcc gccggacccc gcgctcgtca tggagaccga ccggcccgag
     2761 cgcgacggct ggtccaccgg ctcgccggtc acccacctgc gctacgaccc caacgaggac
     2821 cggggcgcgc ggcgctggcg catcgcccgc atcgagctgc gcgccacgga cgccaccagc
     2881 gccaacgcct tcgacatccg ctggcgcgac gactccggcg ccacgggcgt gaaggtgacg
     2941 ctgtcctacc ggcccgagtc cgggggcgcc gagacggcca tcgccggcgg catcgaccag
     3001 cagcccggcg tgaacaccta ccgctgggac accgccggcg tggcgccggg ccgctacgtg
     3061 gtccgggtca ccggcgacga cggcgtcagc cgcggctccg gcacctccac cggcccggtg
     3121 gacgtgcggg cctcgctgcg caccgccggg agcaaccgca tcgccaccgg cgtcgcgctg
     3181 tcccggctgg cctaccccgg cggcgccggc accgccgtgc tcgcccgggc cgacggtttc
     3241 gccgacgcgc tcgccgccgc cccgctggcc gcggccgccg acggcccgct cctgctgaac
     3301 gccggcgacc ggctggaccc ggccgtcgcg gcggaactgg accgcctgaa ggccgccacc
     3361 gtcttcctgc tgggcggcga gtccgcgcag tcctcggccg tcgaggccgc actggaggcc
     3421 tcgacggccg aggactgcgc ggactcgccg cccagcagga agacggtggc ggccttcagg
     3481 cggtccagtt ccgccgcgac ggccgggtcc agccggtcgc cggcgttcag caggagcggg
     3541 ccg
//
LOCUS       DATMKC010000077         6274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_330646, whole genome shotgun sequence.
ACCESSION   DATMKC010000077 DATMKC010000000
VERSION     DATMKC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330646"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VNU01_02205"
     CDS             complement(<1..1551)
                     /locus_tag="VNU01_02205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWH31465.1"
                     /translation="MGPGAQVRVLRGDAERGPREWAAGVVPALSAALAAALLWLWLFA
                     QPGYRRPVTAVVNALVMVVLVAVTASLVRLRDLGPGERRAWRTFLTAFLLFSVSSSAG
                     AYVQLAPEQVPSWLPTLRQVVVVSGFGVMVLALFRLPAAPVRGLAKARFALDTVTIFA
                     SSTIFLWHFVIAPALRARGMTGREAVVVVVAVGFMAVFVGALSMLLLRERTPGTREAL
                     IRLVVGVALVFGVIVASLTAHRPPAVPGPLWSTALTWFAYVLVISAAVQQRRHVMGSE
                     VVDRDVEHRALLALPYVAVALAFAFLLFAGLREAPLELRGVLVSASVVGVCVLIRQRL
                     LLHENELLRRRLVERASHDDLTGLRNRGSWEAEAEREVARARRNGHPLSVLVLDVDDF
                     KTVNDQYGHAAGDLVLRAVGQALREGVRTADLPARLGGDEFVVLLPDTGLEDAVHLAQ
                     AVCRRIERLDVPEAANVAFTVSVGAASMPPASSVRGLVAVADSAMYAAKRDGRNRVRS
                     AESPLSVLG"
     gene            1692..2330
                     /locus_tag="VNU01_02210"
     CDS             1692..2330
                     /locus_tag="VNU01_02210"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH31466.1"
                     /translation="MAEGTADTGGEAAPTRERILEAAFNAVCVHGLGRLSLEDVAREA
                     ELSRQTVYRYFGGKQALVEAVVLREEEQFIERILAATDEHDDVRPALEAAILAALQAA
                     REHPLLDRLLATEPEAMLPLLMTGNGPVLPAARTALEALFARRLPHLSAEEAWRVADA
                     TTRLFISYAINPPEDTSEAVAAGLADLILNGLKDPTNWDPTSTAGQTQGEHP"
     gene            2327..3466
                     /locus_tag="VNU01_02215"
     CDS             2327..3466
                     /locus_tag="VNU01_02215"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWH31467.1"
                     /translation="MSTRHYALPVEATGWTVPGVANVHFNWEYDENREKLLSLYEKGK
                     NKQWNAQERIDWSIEIDIEDQGFTPDEYVSIYGSPMWERMTRKEKDQVRHHMAAWLNS
                     QFLHGEQGALICAARTVETVPDIDAKWYGATQVIDEARHVETYAKYLLEKLELAYPIN
                     PHLKELLNSTLTDSRWDMTYLGMQIMIEGVALAAFSMIRDFTTEPLSKSVNAFVMQDE
                     ARHVAFGLLSLKDAYADMSRAELDEREEFVVEASYLLKDRFLAEEVWDNLGLPVKECM
                     EYADRHQRMSDYRKALFSRIVPNVKKIGLWGPKIQNAFVDMGVMMFEDLDPDEAFARD
                     ESIAEEMDKLIAEGRHREPRGTWETDAEQVAETIAVGYQAEQAGE"
     gene            complement(3509..6214)
                     /locus_tag="VNU01_02220"
     CDS             complement(3509..6214)
                     /locus_tag="VNU01_02220"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWH31468.1"
                     /translation="MRVSVIGVGHVGLVTAACMAELGHDVVGMDDDAAKVATLVQGRV
                     PFMEDGVQELVDRGMAEGRLRFTGSAQEAVRHADVVFVCVGTPRKADGAPNLAYVQQA
                     AASVAEHAERPVVLAEKSTVPVRTGERIRHALALHAQATGSDVTHHVVSNPEFLREGT
                     AVQDTLRPDRVVVGADSDEAHEVMRRLYAPMLERHSCPYVATDVRTAELIKHASNAFL
                     ATKISFINAIARVCELAGADVHTIADAMGHDARIGRAFLNAGLGFGGSCLVSEETVLA
                     RRDGTVRLLTMGEFFAACAATGVAGWEVLSWEPERRTPLFLPVAAVTARPHQGEIVEV
                     RTKMGRRLRCTPDHPFVVGDGRAEAVSLRLAGELSERDWLPLACGSWGEAGGVPERLS
                     VLAAVGAGTVARPDVIQRLDAVQLEAVRGAPGLAPGRRRDVLRRGAMRLGEFERLGVG
                     TPGGRFGTVRNGTCVPGQIPLDADFWRMVGLYLAEGYVTADGRRRRICWTFHPTDEME
                     LADFVVRYWDQLGVKASVHQRQTALAVSVSSRLLASWLLDVLEVGRDAYTKNVPDVAW
                     QAPADHQRALLRGLWDGDGSWSLVSGGPSVVLEYGTVSRRLADAMVRLLGHLGVVARL
                     KVGRTAKSTVDTYWLVISGAEQVEAALWLFPPEEAEEITAHIGRQRKRIAPTGYRRDG
                     EGAAWVRVTDVRREPFDGWVYSLEIPGAHTVVTSGGLVTHNCFPKDVEAFVHIADELG
                     YDFGLLRETDRINREAKAWPLAQLRRLLWNVADKEVAVLGLAFKPGTDDIRDAPALDV
                     IGSLVEEGARVRLHDPVALPQVEGRWDGVTLYDKAEDALRGAHAVVVCTEWDEYRAIP
                     PERLGELLGYPVVVDARNVWDAAALEAAGLTVASVGRPLPEAPGA"
BASE COUNT         1012 a         2360 c         2130 g          772 t
ORIGIN      
        1 ccccaggacc gacagcggcg actccgccga ccggaccctg ttgcggccat cccgcttggc
       61 ggcgtacatg gcggagtcgg ccacggccac gagtccgcgc acggacgagg ccggcggcat
      121 ggacgccgca ccgacgctga ccgtgaaggc cacgttcgcc gcctccggca cgtccaggcg
      181 ctcgatgcgg cggcacaccg cctgggccag gtggaccgcg tcctccagcc ccgtgtccgg
      241 cagcaggacg acgaactcgt cgcctcccag gcgcgccggc aggtcggcgg tgcgcacgcc
      301 ctcgcgcagc gcctgcccca cggcccgcag caccaggtcc ccggcggcgt gcccgtactg
      361 atcgttcacc gtcttgaagt catcgacgtc cagcaccagc acggacagcg ggtggccgtt
      421 gcggcgcgcc ctggcgacct cccgttccgc ctcggcctcc cagctgccgc ggttgcgcaa
      481 gcccgtcagg tcgtcgtgcg aggcgcgctc caccaggcgg cggcgcaaca gctcgttctc
      541 gtgcagcagc agccgctggc ggatgagcac gcacaccccg accaccgacg ccgacaccag
      601 cacgccgcgc agctccaacg gcgcctcgcg caggccggcg aacagcagga aggcgaacgc
      661 cagggcgacg gccacgtacg gcagcgccag gagcgcacgg tgctcgacgt cgcggtccac
      721 gacttcggac cccatgacgt ggcggcgctg ctgcacggcc gccgagatca ccagcacgta
      781 cgcgaaccac gtcagggccg tggaccacag cgggccgggc acggccggcg gccggtgggc
      841 ggtcagcgac gcgacgatca cgccgaacac cagcgccacc ccgacgacga gccggatgag
      901 ggcctcccgc gtcccgggcg tgcgctcccg cagcagcagc atgctcagcg cgcccacgaa
      961 caccgccatg aagcccaccg ccacgacgac cacgaccgcc tcgcgccccg tcatgccacg
     1021 cgcccgcagc gccggggcga tcacgaagtg ccacaggaaa atggtggagg aggcgaagat
     1081 cgtcacggtg tccagggcga agcgcgcctt cgccaggccc ctcaccggcg cggccggaag
     1141 acggaacagc gccagcacca tcacgccgaa gccgctcacg acgaccacct ggcgcagcgt
     1201 gggcagccag gacggcacct gctccggcgc gagctgcacg taggcaccgg ccgaggacga
     1261 cacggagaac agcaggaagg cggtcaggaa cgtgcgccac gcgcgccgct cgcccggccc
     1321 caggtcgcgc aggcgcacca gcgaggcggt caccgccacc aggacgacca tcaccagcgc
     1381 gttcaccacc gccgtgacgg ggcggcgata gccaggctgc gcgaacagcc acagccacag
     1441 gagcgccgcg gccagtgccg cggacagcgc gggcaccaca cccgccgccc attccctcgg
     1501 tccgcgctcg gcgtcgccgc gcaggaccct gacctgcgca cccggaccca tgcccaccat
     1561 ggtcaggccg cacccggtgc cgcggccata gaccgttgtg accatttttg taacgaaacg
     1621 cgaggcggag gtcacgcagc ggcacgcaga aagtgcttgt caccatgtga cagtctccgt
     1681 atgttcgtca catggcggag ggcacagcgg acaccggcgg cgaggccgcc cccacgcgcg
     1741 agcgcatctt ggaggccgcg ttcaacgccg tgtgcgtcca cggcttgggc cggctgagcc
     1801 tggaggacgt cgcgcgcgag gcggagctgt cccgccagac ggtgtaccgc tacttcggcg
     1861 gcaagcaggc gctggtggag gccgtcgtgc tgcgcgagga ggagcagttc atcgagcgca
     1921 tcctcgccgc caccgacgag cacgacgacg tgcggcccgc gctggaggcc gcgatcctcg
     1981 ccgcgctcca ggccgcacgg gagcacccgc tgctcgaccg gctcctggcc accgagccgg
     2041 aggcgatgct gccactgctg atgaccggca acggtcccgt gctgcccgcg gcccgcaccg
     2101 ccctggaggc gctgttcgcc cgccggctgc cgcacctgtc cgcggaggag gcgtggcggg
     2161 tcgccgacgc caccacgcgc ctgttcatca gctacgcgat caacccgccg gaggacacct
     2221 ccgaggcggt cgcggccggc ctggccgacc tgatcttgaa cggactgaag gacccgacga
     2281 actgggaccc cacgagcacg gcggggcaga cacaagggga gcacccatga gcacgcgcca
     2341 ctacgccctc ccggtcgagg ccaccggctg gaccgtgccg ggcgtcgcga acgtccactt
     2401 caactgggag tacgacgaga accgcgagaa gctgctgtcg ctctacgaaa agggcaagaa
     2461 caagcagtgg aacgcccagg agcgcatcga ctggtcgatc gagatcgaca tcgaggacca
     2521 gggcttcacg cctgacgagt acgtgtccat ctacggctcg cccatgtggg agcgcatgac
     2581 gcgcaaggag aaggaccagg tgcgccacca catggccgcc tggctgaact cccagttcct
     2641 gcacggcgag cagggcgcgc tgatctgcgc cgcccgcacg gtggagacgg tgccggacat
     2701 cgacgcgaag tggtacggcg ccacgcaggt catcgacgag gcgcgccacg tcgagaccta
     2761 cgcgaagtac ctgctggaga agctggagct cgcctacccg atcaacccgc acttgaagga
     2821 gctgctcaac tccacgctca ccgacagccg ctgggacatg acctacctgg gcatgcagat
     2881 catgatcgag ggcgtggcgc tggcagcgtt cagcatgatc cgcgacttca ccaccgagcc
     2941 gctgagcaag tcggtgaacg ccttcgtgat gcaggacgag gcccggcacg tcgcattcgg
     3001 cctgctgtcg ctcaaggacg cctacgcgga catgtcgcgg gccgagctgg acgagcgcga
     3061 ggagttcgtg gtcgaggcga gctacctgct caaggaccgc ttcctggccg aggaggtctg
     3121 ggacaacctg ggcctgcccg tcaaggagtg catggagtac gccgaccgcc accagcgcat
     3181 gagcgactac cgcaaggccc tgttcagccg catcgtgccc aacgtgaaga agatcgggct
     3241 gtgggggccc aagatccaga acgcgttcgt ggacatgggc gtgatgatgt tcgaggacct
     3301 ggacccggac gaggccttcg cgcgcgacga gtccatcgcc gaggagatgg acaagctcat
     3361 cgccgagggg cgccaccgcg agccgcgcgg cacctgggag accgacgccg agcaggtcgc
     3421 cgagaccatc gccgtgggct accaggccga gcaggccggc gaatagacca ccacccgcac
     3481 cgggtgccgg gagcctgcct accccccgtc aggctcccgg cgcctcgggt aacggccgcc
     3541 cgacgctcgc cacggtgagg ccggccgcct ccagggcggc cgcgtcccag acgttgcggg
     3601 cgtccaccac gacgggatag ccgagcagct cgccgaggcg ctccggcggg atcgcgcggt
     3661 actcgtccca ctcggtgcag accaccacgg cgtgcgcgcc gcgcagggcg tcctccgcct
     3721 tgtcgtacag ggtgacgccg tcccagcggc cttcaacctg cggcagtgcc acggggtcgt
     3781 gcaggcgcac gcgggcgccc tcctccacca ggctgccgat cacgtccagc gccggcgcgt
     3841 cgcggatgtc gtcggtgccc ggcttgaacg ccaggcccag gacggccact tccttgtccg
     3901 cgacgttcca cagcaggcgg cgcagctgcg ccagcggcca cgccttggcc tcgcggttga
     3961 tgcggtcggt ctcgcgcagc aggccgaagt cgtagccgag ctcgtcggcg atgtgcacga
     4021 acgcctcgac atctttgggg aagcagttgt gcgtcaccag cccgccgctg gtcaccacgg
     4081 tgtgcgctcc cgggatctcc agcgagtaca cccagccgtc gaagggctcc cgtcgcacgt
     4141 cggtgacgcg cacccaggcc gcgccctcgc cgtccctgcg gtaaccggtc ggcgcgatgc
     4201 gcttgcgctg ccgtccgatg tgggcggtga tctcctcggc ctcctcaggc gggaacagcc
     4261 aaagcgccgc ctcgacctgt tccgcgcccg agatcaccag ccaatacgta tcgaccgtcg
     4321 acttggcggt ccggcccacc ttcaaccgtg cgaccacacc aaggtgcccg aggagccgca
     4381 ccatggcgtc ggcgagcctt cgggagacgg tcccgtactc cagcacgacg ctcgggccgc
     4441 cggagacgag cgaccagctc ccgtccccgt cccacagtcc gcgcagcagt gcgcgctggt
     4501 ggtctgccgg cgcttgccag gcgacgtcgg gaacgttctt ggtgtaggcg tcccggccga
     4561 cctcgagcac gtcgagcagc catgaggcca gcagcctgct cgagacggac accgccaggg
     4621 cggtctggcg ctggtggacc gatgccttga ccccgagctg gtcccagtac cgcacaacga
     4681 agtcggcgag ctccatctcg tcggtgggat ggaaggtcca gcagatccgt cgacggcgcc
     4741 cgtccgcggt gacgtagcct tccgcgagat agaggcccac catgcgccag aagtccgcgt
     4801 cgagcggaat ctggcccggc acgcacgtcc cgttgcgcac ggtgccgaag cgaccgcccg
     4861 gcgtcccgac ccccaggcgc tcgaactcgc ccagccgcat cgcgcccctg cgcaggacat
     4921 cgcggcgccg accgggggcc agtcccgggg ccccgcgcac cgcctccagc tgcacggcgt
     4981 cgagacgctg gatcacgtcc gggcgcgcga cggtcccggc accgacggcc gccagcacgc
     5041 tcagccgctc cggcaccccg ccagcctcgc cccaggagcc acacgcgagc ggcagccagt
     5101 cccgctcgct cagctcgccg gcgaggcgga gcgacacagc ctccgcgcgg ccgtccccga
     5161 ccacgaacgg gtggtccggt gtgcagcgca ggcggcgtcc catcttcgtg cggacctcga
     5221 cgatctcacc ctgatgcggc cgcgcggtca ccgccgccac cgggaggaac agcggcgtcc
     5281 ggcgttcggg ctcccaggaa aggacctccc accccgccac gccggtcgcc gcgcacgcgg
     5341 cgaagaattc gcccatggtg agcagacgca ccgtcccgtc gcgtcgcgcg agcaccgtct
     5401 cctcggacac caagcacgag ccgccgaagc caagtccggc gttgaggaac gcgcggccga
     5461 tgcgggcgtc gtgccccatc gcgtcggcga tcgtgtgcac gtcggcgccg gcgagctcgc
     5521 acacgcgcgc gatcgcgttg atgaagctga tcttggtggc caggaacgcg ttgctggcgt
     5581 gcttgatgag ttccgccgtg cgcacgtcgg tggcgacgta cgggcacgag tggcgctcca
     5641 gcatcggcgc gtacagccgg cgcatgacct cgtgcgcctc gtcgctgtcc gcgccgacca
     5701 ccacgcggtc gggccggagg gtgtcctgga ccgccgtgcc ctcgcgcagg aactcggggt
     5761 tgctgaccac gtggtgcgtg acgtcgctgc cggtggcctg cgcgtgcagc gcaagcgcgt
     5821 ggcggatgcg ctcgccggtg cgcaccggga ccgtcgactt ctccgccagc accaccggcc
     5881 gctcggcgtg ctcggcgacg ctcgccgccg cctgctgcac gtaggccagg ttcggcgcgc
     5941 cgtcggcctt gcgcggcgtg ccgacgcaga cgaacaccac gtcggcgtgg cgcaccgcct
     6001 cctgcgcgct gcccgtgaag cgcaggcgtc cctcggccat gccgcggtcg accagctcct
     6061 gcacgccgtc ctccatgaac gggacccggc cctgcaccag cgtggcgacc ttcgcggcgt
     6121 cgtcgtccat gccgaccacg tcgtggccca gctcggccat gcaggcggcg gtgaccagac
     6181 cgacgtggcc caccccgatc acgctgaccc tcacgatggc gaatgctcct cccccggcac
     6241 ggggctaggc gtcctccagc cagacggtgt ccac
//
LOCUS       DATMKC010000078         9162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_110424, whole genome shotgun sequence.
ACCESSION   DATMKC010000078 DATMKC010000000
VERSION     DATMKC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110424"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VNU01_02225"
     CDS             <1..1251
                     /locus_tag="VNU01_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31469.1"
                     /translation="GELATLTAHWEAEKQAIARIRELKQDIEAARERADRAERDGELQ
                     TAAELRYGRIPGLERDLEQANAALAELQSTQRMLKEEVDAEDVAEVVSRWTGIPVSRL
                     VEGERDKLVRLEDTLRERVIGQDEAVQAVADAVRRSRAGLADPDRPLGSFLFLGPTGV
                     GKTELARALAEFLFDDERAMVRLDMGEYGEKHTVSRLIGAPPGYVGYEEGGQLTEPVR
                     RRPYSVILLDEVEKAHADVFNTLLQVLDDGRLTDGQGHTVDFRNTVVIMTSNLGSQWI
                     LDPIESEQVVRERVMAAVREHFRPEFLNRLDEILIFHRLALPQLRAIVDVQLERVAGR
                     FAQRDLKLEVSPAARDWLADRGYDPVYGARPLKRVLRKELEDKVALALLNGSIAEGDT
                     VEVDVADGALSVAGSSGAGARAPR"
     gene            complement(1205..3943)
                     /locus_tag="VNU01_02230"
     CDS             complement(1205..3943)
                     /locus_tag="VNU01_02230"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HWH31470.1"
                     /translation="MAVPADLLTQRLPWAAAADSGAAANVELRDQLDTYFPVQRELVQ
                     RLRAGEGADWLHDVSTGVPGTAFIGWGLWSPFNLPAAVLDFSLAWAWGMALRLFAAMA
                     GAYLLVRRLGAGRLGGTVAGAAFGLSGFMVLWLGWPQSHVAAVAPWVWWATHRTVAED
                     APWWAGPALAVSTAALWLGGFPAVSAYVLAAAGAVALHTAWPLRHRRRAALRGLGAAA
                     LAVSLGTGAAAFTLLPSLRILEEFSLGYRDQAWRTGLPTRALLTFVAPGFFGDVVHHR
                     LWLGVSYVEAVAYTGVVSLLLGCLAWTVAPRRRGLGLFTAMAAGGLLLVYGLPPLRTL
                     LSLAPMLRSNVPTRALAVVSLALAVLGGLGADALRRLLRGEAAWDRRAARATAVVAAG
                     AGLAYVAMHPPGRLRELAYRRLPQEVAQRAGDVAGWEAAAAFGFVLLAAAVVTGALWL
                     RPRAPRRAEAVLTGGLVAVLAWDLLAFAGGWNVQAPPAQLFPPVAAIQHLARTSVNHH
                     VAGADDVLMPNTHLAYGVADIRAHAFVTRRFTDVLARLDARRQSPTLWDLQAVDSGQW
                     RPWLPLLGVREVLIPDSGPRLGATLVRERHDDVGPALTEGVVHELEATAPRGGTLEGV
                     LLRVGTYGRANDGELVVRVEGPAGAARGAVVAAGLRDLAWTFVPVTPLDVEEGERLRI
                     EVEGTAARADQAVSVFGHRDGEEFTPALALHHGEELPSRPLADADGVRVVELPDALPL
                     VFAAKEARPSAEGGALGAQALQVAQRDVVVVEPYGGRELPALPDGDGTAEVVSWSRAG
                     GRVQAEVRSDRGAVVTTLDEALEGWTATVDGRPATVVRVNHLFTGVVVPRGVHTVELR
                     YRAPLTAAGLVTSGRSVGAIVGWALLRRLRDRTGMSTRQLLLTAAHELQRRWIQRR"
     gene            4152..4964
                     /locus_tag="VNU01_02235"
     CDS             4152..4964
                     /locus_tag="VNU01_02235"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWH31471.1"
                     /translation="MYEFDEDTAVRVQADGSYRGTVTDRWSVGDRPNGGYLMAIGLRA
                     MAQTLAQPDPLTVTTHFLRSPAPGPVAVEVEVVRKGRTVETAEARLHSGDREHVRMLA
                     TFGDLRAATGPTQVLLRAPALPGPERCVPLPSEAKLPDGTTATIMQRFDVRMTPESVG
                     WVMGRPSARAEAQGWVRFRDDRPADLISLVQVLDAFAPAVLAAGHRGSVPTIELTAHF
                     RAHPAPGWLRLVTRTNVLVDGYLEEEAEVWDAEDRLVAQARQFARLAGAGEG"
     gene            4991..5923
                     /locus_tag="VNU01_02240"
     CDS             4991..5923
                     /locus_tag="VNU01_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWH31472.1"
                     /translation="MSAPPESSLPPASGLPRAPVPLWRRVLRVLWLVVVLVTLALVLR
                     RRGSGVLDALGDVSAWSLAGAALAVTAGVGASAQVWRGILGGMGVHLPWRAGARVFFV
                     GQLGKYLPGSVWPLVAQMELGRDYGVGRRVSVGALGLFMVVHLLSAGIVAAIALPVAG
                     LVAWPWLLAAPPLLALLLPRPLGSALRLAFRVLRRPAPALPGGRALAVALGWALCMWA
                     LYGLHLWLLARDAGADLGLLGAVGAFALAWAAGFLFVVAPVGAGAREAVLVGIAAGTG
                     GTGVLLAVALLSRALSTVADALWGVAALAVRRSS"
     gene            complement(5907..6542)
                     /locus_tag="VNU01_02245"
     CDS             complement(5907..6542)
                     /locus_tag="VNU01_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWH31473.1"
                     /translation="MTDVRAQAETPGVVIGNHHHKYTSSNPAIRFLTERFLERLESMF
                     AQVQAGAPDARVLEVGCGEGEIAQRLRARWPEVTGLDLPDAGLRQAWDGVDGVRFLHG
                     DAERLPFPDGAFDVVVAVEVLEHLRDPAQGLRELARVSRGHLVLSVPREPIFRTGNLL
                     AGRHVRALGNTPGHLNHWSTAGFVRFASQAGAVRAVAKPLPWTIAWVRKNV"
     gene            complement(6535..7425)
                     /locus_tag="VNU01_02250"
     CDS             complement(6535..7425)
                     /locus_tag="VNU01_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWH31474.1"
                     /translation="MESPPIDLDVTVVLPAFNEAGHIGVEVERITQALKDSGRTFEIL
                     VVDDGSTDGTAELVEGLPFVRVLRFPVNRGSGTARRIGTAQARGRWVVWTDADLTYPN
                     ERIPELVDVLASGRAQQVVGARTSEEGTYKLLRVPAKWAIRRLAEFLTATRIPDLNSG
                     LRAFAREDARPYLGLLPAGFSCVTTITLAFLANGLVVEYVPIEYRPRSGRSHFHPIKD
                     AYRYILQVLRMVTFFEPLRVFAPVALTLLAGGAVKFVVDIVRHDFRVATNTVLIFVTG
                     MILFSLGLLADLIVRVSRHD"
     gene            7491..8231
                     /locus_tag="VNU01_02255"
     CDS             7491..8231
                     /locus_tag="VNU01_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH31475.1"
                     /translation="MTILAWQRGGCAGGPAVLLLHGWASDGRSDFEAAGWVTALGPAG
                     YEALVPDLPGHAASADVALPPNTDPAGWSAGVVVQDLDRLGVGAVRLVGYAEGCLVAG
                     HLAARHPERMRPLVLLSRDDTVGVRGGAQVAALLREERAGLWDAEAAEVAARARRDGW
                     HHGPALADWVERAVWPAAPRLAALDMPVLLAVGADDEHRHGAPRLAQLFHDARLVTVP
                     GDARTMRKAPETIEAVVAFLKEHEPEGA"
     gene            8234..8614
                     /locus_tag="VNU01_02260"
     CDS             8234..8614
                     /locus_tag="VNU01_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWH31476.1"
                     /translation="MDRSRIAELAALAGRGFEGEAEDAVEAFTDDAELQDQPGEELVS
                     GRPALEGFFLDYAGRRARFALREVVADGDRAAVAYDLWFRSDSTAYGQHGMALLRLRD
                     DRIASWRGVWVQTAGHDLSPWDFD"
     gene            8620..>9162
                     /locus_tag="VNU01_02265"
     CDS             8620..>9162
                     /locus_tag="VNU01_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HWH31477.1"
                     /translation="MVALVLAAPLVLVLVLVVEAVIARRRDYPFEAFERIQHRTAGQG
                     DPLRVAMLGDSTVAGVGVLQVAETLPVQTAERVAAALGRPVEVTGFGVSGARTRQVAG
                     MQGEELDRFDVVVVVIGSNDVTGLTPPWRFRDQTRALLAELPKPAVLGGIPLFGGATA
                     IPRPLRDLVRAYAGVLRGVQV"
BASE COUNT         1144 a         3441 c         3467 g         1110 t
ORIGIN      
        1 ggcgagctcg cgacgctgac cgcccactgg gaggcggaga agcaggcaat cgcgcgcatc
       61 cgggagctca agcaggacat cgaggcggcg cgcgagcgcg ccgaccgcgc cgagcgcgac
      121 ggcgagctgc agaccgccgc ggagctgcgc tacgggcgca tcccgggcct ggagcgggac
      181 ctggagcagg ccaacgcggc gctggccgaa ctgcagtcca cccagcgcat gctcaaggag
      241 gaggtcgacg ccgaggacgt cgccgaggtg gtcagccgct ggaccggcat cccggtgagc
      301 aggctggtcg agggcgagcg cgacaagctc gtgcgcctgg aggacacctt gcgcgagcgg
      361 gtgatcgggc aggacgaggc cgtccaggcc gtcgcggacg cggtgcgccg ctcgcgcgcc
      421 gggctggccg acccggaccg gcctttgggc tcgttcctgt tcctggggcc gaccggcgtg
      481 ggcaagaccg agctggcccg cgcgctggcg gagttcctgt tcgacgacga gcgcgccatg
      541 gtgcgcctgg acatgggcga gtacggcgag aagcacaccg tgtcgcgcct gatcggcgcg
      601 ccccccggct acgtcggcta cgaggagggc ggccagctca ccgagccggt gcgccgccgc
      661 ccctacagcg tgatcctgct ggacgaggtc gagaaggccc acgccgacgt gttcaacacg
      721 ctgctgcagg tgctggacga cggccggctc accgacggcc agggccacac ggtggacttc
      781 cgcaacaccg tggtgatcat gacctccaac ctgggctcgc agtggatcct ggatcccatc
      841 gagagcgagc aggtcgtgcg cgagcgcgtc atggccgccg tgcgcgagca cttccgcccg
      901 gagttcctca accggctgga cgagatcctg atcttccacc ggctcgcgct gccgcagctg
      961 cgcgcgatcg tggacgtgca gctggagcgc gtggccggcc gcttcgccca gcgcgacctg
     1021 aagctggagg tcagcccggc ggcccgggac tggctggccg accgcggcta cgaccccgtc
     1081 tacggcgccc ggccgctgaa gcgcgtgctg cgcaaggagc tggaggacaa ggtggcgctc
     1141 gcgctgctga acggctcgat cgccgagggc gacaccgtgg aggtggacgt cgccgacggc
     1201 gcgctcagcg tcgctggatc cagcggcgct ggagctcgtg cgccgcggtg agcagcagct
     1261 ggcgcgtgct catgccggtg cggtcgcgca gccggcgcag cagcgcccag cccacgatcg
     1321 cgcccacgga gcggccgctg gtgaccagcc cggcggcggt gagcggcgcc cggtagcgca
     1381 gctccacggt gtgcaccccc cgcggcacca ccacgccggt gaacaggtgg ttgacccgca
     1441 ccacggtcgc cggtcggccg tccacggtcg cggtccaccc ctcgagggct tcgtcgagcg
     1501 tggtcaccac cgcgccgcgg tcactgcgga cctcggcctg gacgcgcccg ccggcccggc
     1561 tccacgacac cacctcggcc gtgccgtcgc cgtccggcag cgccggcagc tcccggcccc
     1621 cgtacggctc caccacgacg acgtcgcgct gggccacctg gagcgcctgg gcgcccagcg
     1681 cgccgccctc cgcggacgga cgcgcctcct tcgccgcgaa caccagcggc agggcgtcgg
     1741 gcagctccac cacccggacg ccgtcggcgt cggccagcgg ccggctgggc agctcctcac
     1801 cgtggtgcag cgcgagggcc ggcgtgaact cctccccgtc gcggtggccg aacacgctga
     1861 ccgcctggtc ggcgcgtgcg gcggtgccct cgacctcgat gcgcaaccgc tcgccctctt
     1921 ccacgtccag cggcgtcacc gggacgaacg tccacgccag gtcgcgcagc ccggcggcca
     1981 ccacggcgcc gcgcgcggcg ccggcaggac cttcgacgcg caccacgagc tcgccgtcgt
     2041 tcgcgcgccc gtacgtgccg acgcgcagca gcacgccttc cagcgtgccg cccctggggg
     2101 cggtggcctc cagctcgtgt acgacgccct cggtcagcgc cggacccacg tcgtcgtggc
     2161 gctcgcggac cagcgtggcg cccaggcgcg gcccgctgtc gggaatcagc acctcccgca
     2221 cccccagcag cggcagccat ggccgccact ggccgctgtc cacggcctgg aggtcccaca
     2281 gggtcggcga ctggcggcgc gcgtccaggc gcgccagcac gtccgtgaag cggcgcgtca
     2341 cgaacgcgtg ggcgcggatg tcggcgacgc cgtaggccag atgcgtgttg ggcatcagga
     2401 cgtcgtccgc cccggccacg tggtggttca ccgaggtgcg cgccaggtgc tggatggcgg
     2461 ccacgggcgg gaagagctgc gcgggaggcg cctggacgtt ccagcccccg gcgaacgcca
     2521 gcaggtccca tgccagcacc gccaccagcc cgccggtcag caccgcctcc gcacgacgcg
     2581 gcgcgcgcgg ccgcagccac agcgccccgg tcacgaccgc cgcggccagc agcacgaagc
     2641 cgaacgccgc cgcggcctcc cagcccgcga cgtcgccggc gcgctgcgcg acctcctgcg
     2701 gcaggcgccg gtacgccagc tcccgcaggc ggcccggtgg gtgcatcgcg acgtaggcca
     2761 gcccggcacc ggcggcgacc acggcggtcg cgcgcgcggc gcgccggtcc cacgccgcct
     2821 cgccgcgcag caggcgccgc agcgcgtcgg cgccgaggcc gcccaggacc gccagcgcca
     2881 ggctcaccac cgcgagcgcg cgcgtgggca cgttgctgcg cagcatcggc gccagcgaca
     2941 gcagcgtccg cagcggcggc aggccgtaca ccagcagcag cccgccggcc gccatggcgg
     3001 tgaacagtcc caggccgcgg cggcgcggcg cgaccgtcca cgccaggcag cccagcagca
     3061 gcgagaccac gccggtgtag gcgaccgcct ccacgtagct cacgcccagc cacagtcggt
     3121 ggtgcaccac atcgccgaag aagccgggcg ccacgaacgt cagcagcgcc cgcgtcggca
     3181 gcccggtgcg ccaggcctgg tcgcggtagc ccaggctgaa ctcctccagg atccgcagcg
     3241 acggcagcag cgtgaacgcg gctgcccccg tgcccagcga gacggccaag gcggccgcgc
     3301 ccagcccgcg cagcgccgcg cggcgcctgt gccgcagggg ccaggccgtg tgcagcgcga
     3361 ccgccccggc ggcggccagc acgtaggccg acacggcggg aaacccgccg agccacagcg
     3421 ccgcggtcga cacggccagc gccgggccgg cccaccacgg cgcgtcctcg gcgacggtcc
     3481 ggtgcgtcgc ccaccacacc cacggggcga cggcggcgac gtgcgactgc ggccagccca
     3541 gccacagcac catgaagccc gacaggccga aggccgcgcc cgccacggtg ccgccgagcc
     3601 gccccgcgcc cagccgccgc acgagcaggt aggcgccggc catggcggcg aacagccgca
     3661 gggccatgcc ccaggcccag gccagtgaga agtccagcac cgcggccggg aggttgaacg
     3721 ggctccacag cccccagccg atgaacgccg tgccggggac gccggtgctg acgtcgtgca
     3781 gccagtcggc gccctcgccg gcgcgcaggc gctggaccag ctcacgctgc accgggaagt
     3841 aggtgtccag ctggtcgcgc agctccacgt tcgccgccgc gccgctgtcc gccgccgccg
     3901 cccagggcag gcgctgggtg agcaggtcgg cgggcaccgc cacccgggcg ccggtcagcg
     3961 acggccccag gacggcggcg accaggacgg cgaagacggc gaacggccac gcgcgcgtca
     4021 gcggtccgac ggaacgcaga tcgacctccg aggctctcca gcggctctgc agggggcggc
     4081 aagcctaacc gccgttcgcg gcacactgcc ggttggtgcg ctgacccggg gcgtctacca
     4141 tcagcggcgc catgtacgag ttcgacgagg acaccgcggt ccgcgtccag gcggacgggt
     4201 cgtaccgcgg gaccgtgacg gaccggtgga gcgtggggga tcgcccgaac ggggggtatc
     4261 tcatggccat cggtcttcgc gcgatggccc agacgctcgc ccagccggac ccgctcacgg
     4321 tgaccaccca cttcctgcgc tctcccgcgc ccggcccggt ggctgtggag gtcgaggtgg
     4381 tgcgcaaggg ccgcaccgtg gagacggccg aggcccggct gcactccggc gaccgtgagc
     4441 acgtgcgcat gctggccacg ttcggcgacc tgcgcgccgc gaccgggccc acgcaggtcc
     4501 tcctgcgcgc acccgcactg ccgggccccg agcgctgcgt gccgctgccg tcggaagcca
     4561 agctgcccga cggcacgacc gcgacgatca tgcagcgctt cgacgtccgc atgaccccgg
     4621 agtcggtggg ctgggtgatg ggccgcccgt cggcgcgcgc cgaggcccaa gggtgggtgc
     4681 gcttcaggga cgaccggccc gccgacctga tcagcctggt gcaggtgctg gacgccttcg
     4741 cgccggccgt cctggccgcc ggccatcgcg gcagcgtgcc gaccatcgag ctcaccgctc
     4801 acttccgcgc ccatccggcc cccggatggc tgcggctggt cacgcgcacc aacgtcctgg
     4861 tcgacggcta cctggaggag gaggccgagg tgtgggacgc cgaggaccgc ctggtcgccc
     4921 aggcccgcca gttcgcccgg ctcgcggggg caggcgaggg ctgagccccg ccttgcgtac
     4981 cctgcgcggc gtgagcgcgc ctcccgagtc ctctctcccc ccggcctccg gccttccgcg
     5041 cgcccccgtc ccgttgtggc gccgggtgct gcgcgtgctg tggctggtgg tcgtgctggt
     5101 gacgctcgcc ctggtgctgc gccggcgcgg cagtggcgtg ctggacgcac tcggcgacgt
     5161 gtccgcctgg tcgctcgccg gcgccgcgct ggcggtcacg gccggcgtgg gcgcgagcgc
     5221 ccaggtgtgg cgcggcatcc tcggcgggat gggcgtccac ctgccgtggc gggcgggcgc
     5281 gcgcgtgttc ttcgtgggcc agctcggcaa gtacctgccg ggcagcgtct ggccgctggt
     5341 cgcgcagatg gagctcggcc gcgactacgg cgtgggccgg cgcgtctccg tcggcgcgct
     5401 gggcctgttc atggtcgtgc acctgctgag cgccggcatc gtcgccgcga tcgcgctgcc
     5461 ggtcgccgga ctggtcgcgt ggccgtggct gctcgccgcg ccgccgctgc tggcgctgct
     5521 gctgccgcgc ccgctgggct ccgccctgcg gctggccttc cgggtcctgc gccgccccgc
     5581 gcccgcgctg ccgggcgggc gggcgctggc cgtggcgctc ggctgggcgc tgtgcatgtg
     5641 ggcgctgtac gggctgcacc tgtggctgct cgcgcgcgac gccggcgcgg acctgggcct
     5701 tttgggcgcg gtgggcgcgt tcgcgctggc gtgggccgcg ggcttcctct tcgttgtcgc
     5761 gccggtgggc gcgggcgcgc gcgaggccgt gctggtcggg atcgcggccg gcacgggcgg
     5821 gaccggcgtg ctgctggcgg tggcgctgct gtcgcgggcg ctgagcacgg tggcggacgc
     5881 gctgtggggc gtggcggccc tggccgtcag acgttcttcc tgacccaggc gatcgtccac
     5941 ggcagcggct tcgccacggc gcgcacggcg ccggcctggg acgcgaaccg cacgaacccc
     6001 gcggtgctcc agtggttgag gtgccccggc gtgttgccca gggcgcgcac gtggcgcccg
     6061 gccagcaggt tgccggtgcg gaagatcggc tcgcgcggca ccgacaggac caggtggccg
     6121 cggctgaccc gcgcgagctc gcgcagcccc tgtgccgggt cgcgcaggtg ctccagcacc
     6181 tccacggcca ccacgacgtc gaacgcgccg tccgggaagg gcagccgctc cgcgtcaccg
     6241 tgcaggaagc gcacgccgtc cacgccgtcc caggcctgcc gcaggcccgc gtccggcagg
     6301 tccaggccgg tcacctccgg ccagcgggcg cgcagccgct gcgcgatctc gccctcgccg
     6361 cagccgacct ccagcacgcg ggcgtccggc gcgccggcct gcacctgggc gaacatcgac
     6421 tccaggcgct ccaggaaccg ttccgtgagg aagcggatcg ccgggttgga cgaggtgtac
     6481 ttgtgatggt ggttgccgat caccacaccg ggtgtctccg cctgcgcccg cacgtcagtc
     6541 atggcggctc acgcgcacga tgaggtccgc cagcagccca aggctgaaca ggatcatccc
     6601 ggtcacgaag atcaggaccg tgttggtggc cacgcggaag tcgtggcgca cgatgtcgac
     6661 gacgaacttc acggcgccgc ccgccagcag cgtgagcgcc acgggggcga acacgcgcag
     6721 gggctcgaag aacgtcacca tccgcaggac ctggaggatg tagcggtagg cgtccttgat
     6781 cggatggaag tggctgcgcc cggaccgcgg ccggtactcg atcggcacgt actcgaccac
     6841 gaggccgttg gccaggaacg ccagggtgat cgtggtcacg caggagaacc cggcgggcag
     6901 caggcccagg tacgggcggg cgtcctcccg cgcgaaggcg cgcaggccgg agttcagatc
     6961 ggggatgcgc gtcgcggtga ggaactccgc cagccggcgg atcgcccact tggccggtac
     7021 gcgcagcagc ttgtacgtgc cctcttcgct ggtgcgcgcg ccgactacct gctgcgcgcg
     7081 cccggaggcc agcacgtcga ccagctcggg gatgcgctcg ttggggtagg tcaggtccgc
     7141 gtccgtccag accacccagc ggccgcgcgc ctgtgccgtg ccgatgcggc gtgccgtgcc
     7201 ggagccgcgg ttcaccggga agcgcaggac ccggacgaac ggcagcccct cgaccagctc
     7261 ggcggtgccg tcggtggagc cgtcgtcgac caccaggatc tcgaacgtgc gcccggagtc
     7321 cttcagtgcc tgggtgatcc gctccacctc cacgccgatg tgccccgcct cgttgaaggc
     7381 gggcagcacg acggtgacgt cgaggtcgat ggggggggac tccacccgat gagcataacc
     7441 gggtccctgg cctccccgcc tccggcgacg ccccctcgta gcatcccgcc atgaccatcc
     7501 tcgcctggca gcgtggcgga tgcgccgggg gtcccgcggt gctgctgctg cacggctggg
     7561 caagcgacgg gcgcagcgac ttcgaggcgg cgggctgggt caccgcgctg ggcccggccg
     7621 gctacgaggc gctcgtgccg gacctgcccg gccatgccgc ctcggccgat gtggcactgc
     7681 cgcccaacac cgacccggca ggctggtcgg ccggcgtggt ggtccaggat ctcgaccggc
     7741 tgggcgtcgg tgcggtgcgg ctggttggct acgcggaggg ctgcctggtg gccgggcacc
     7801 tggccgcccg ccatcccgag cgcatgcggc cgctggtgct gctgtcgcgc gacgacaccg
     7861 tgggcgtgcg cggcggcgcg caggtcgcgg cgctgctgcg cgaggagcgc gccggcctgt
     7921 gggacgccga ggccgcggaa gtcgccgccc gcgcgcgccg cgacgggtgg caccacggcc
     7981 ccgcgctggc ggactgggtc gagcgggcgg tgtggccggc cgccccgcgc ctggccgccc
     8041 tggacatgcc ggtcctcctg gccgtcggcg cggacgacga gcaccgccac ggcgcaccca
     8101 ggctggcgca gctcttccac gacgcgcggc tggtcacggt gcccggcgac gcgcgcacga
     8161 tgcggaaggc gccggagacc atcgaggccg tggtggcgtt cctgaaggag cacgagccag
     8221 aaggagcgtg agggtggacc gcagcaggat cgctgagctg gccgcactgg cgggccgcgg
     8281 cttcgagggc gaggccgagg acgccgtgga ggcgttcacc gacgacgccg agctgcagga
     8341 ccagccgggg gaggagctgg tctccggccg cccggcgctg gagggcttct tcctggacta
     8401 cgcgggccgc agggcgcgct tcgcgctgcg cgaggtggtc gcggacggcg accgcgccgc
     8461 cgtcgcctac gacctgtggt tccgctccga ctcgaccgcc tacggccagc acggcatggc
     8521 gctgctgcgg ttgcgcgacg accgcatcgc ctcgtggcga ggcgtgtggg tgcagaccgc
     8581 ggggcatgac ctgtccccgt gggacttcga ttgagacgcg tggtcgcgct cgtgctggcc
     8641 gcgcccctcg tgctcgtgct ggtcctggtc gtcgaggcgg tcatcgcccg gcggcgcgac
     8701 tatcccttcg aggcgttcga gcgcatccag caccgcaccg cggggcaggg cgacccgctg
     8761 cgcgtcgcga tgctcggcga ctccaccgtc gccggggtcg gcgtgctgca agtggccgag
     8821 acgctgccgg tgcagaccgc tgagcgggtg gcggcggcac tgggccggcc ggtcgaggtc
     8881 accggcttcg gcgtgtcggg ggcgcgcacg cgtcaggtgg cggggatgca aggggaggag
     8941 ctggaccgct tcgacgtggt ggtggtcgtc atcggctcca acgacgtgac cggcctcacg
     9001 ccgccctggc ggttccgcga ccagacccgg gcgctgctcg ccgagctgcc gaagccggcc
     9061 gtcctgggcg gcatcccgct gttcggcggc gccaccgcga tcccgcgccc gctgcgcgac
     9121 ctggtgcgcg cctacgccgg cgtgctgcgc ggcgtgcagg tg
//
LOCUS       DATMKC010000079         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_350017, whole genome shotgun sequence.
ACCESSION   DATMKC010000079 DATMKC010000000
VERSION     DATMKC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350017"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VNU01_02270"
     CDS             complement(<1..2078)
                     /locus_tag="VNU01_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWH31478.1"
                     /translation="MGAGAETRTALRVCPFCEATCGLSLTLEGDRVTAVHGDDEDVFS
                     RGYLCPKGVAIGELHDDPDRLREPLVRRGGRLEPAGWDEAFEAADDALNRLIERHGRE
                     ALAIYFGNPTTHNVSGPLYNPVLARAAGTRSIFSASTLDQMPKHVSVGLMYGDKFSIP
                     VPDIDRCDYLVVMGADPFTSNGSLWTVPDLPGRMRALKARGGRLVVIDPRRSRTARAA
                     DRHLAILPGTDALLLLGVVHTLFAEDLVRLRRLDGLVRGIDDVRAVAAPYAPETVAAR
                     CGVAAEDIRTLARELAAAERGCLYGRIGTTTQRFGTLTSWLIEVVNVLCGLLDHEGGP
                     MFPLAAAGHRNALPGPARPLELHRWRSRVSGLGEVYGELPSACLAEEIDTPGDGQIRG
                     LITVAGNPARSAPGGPRLDAALADLEAMVCVDIYLNETTRHADVILPPPSPLARPHFD
                     LAFNQLAVRNTARWSPPALSPDPGQPDEWEILLRLTGILTGQGPKADVGAVDAFVART
                     VIQREIAHPASPLHGRDPDELVALLEPRRGPERLIDFLLRSGPYGDAFGARDGLSLQA
                     LLDAPHGVDLGPLRPRTPEVLLNGERAIELAPAPVLEDLPRLAAWAQADGAPLTLVGR
                     RHLRSNNSWMHNLPLLNSGTNRCTLHVHPDDAAAAGLADGSLAQVTARTGSVRVEVEV
                     TEDIRPGVVS"
BASE COUNT          295 a          863 c          877 g          309 t
ORIGIN      
        1 atgctcacca cgccggggcg gatgtcctcg gtgacctcca cctccacccg caccgagccc
       61 gtgcgcgcgg tgacctgcgc caggctcccg tcggccagcc cggccgccgc cgcgtcgtcg
      121 gggtggacgt gcagcgtgca gcggttggtg ccggagttga gcagcggcag gttgtgcatc
      181 cacgagttgt tggagcgcag gtgccgccgg ccgaccagcg tcagcggcgc cccgtccgcc
      241 tgcgcccacg ccgccaggcg cggcaggtcc tccagcaccg gtgcgggagc cagttcgatc
      301 gcgcgctcgc cgttgagcag cacctccggc gtgcgcggcc gcagcggccc caggtccacg
      361 ccgtgtggcg cgtcgagcaa cgcctgcagg ctcaggccgt cgcgtgcccc gaacgcgtcg
      421 ccgtacgggc cgctgcgcag caggaagtcg atcagccgct cggggccccg gcgcggctcg
      481 agcagcgcca cgagctcgtc ggggtcgcgg ccgtgcaggg gagaggccgg gtgggcgatc
      541 tcgcgctgga tcaccgtgcg ggccacgaac gcgtcgacgg cgccgacgtc ggccttcggt
      601 ccctgtcccg tcaggatgcc ggtgaggcgc agcaggatct cccactcgtc cggctgcccg
      661 gggtccgggc tcagcgccgg cggcgaccag cgcgccgtgt tgcgcaccgc gagctggttg
      721 aacgcgaggt cgaagtgcgg ccgcgccagc ggcgacggcg gcggcaggat cacgtcggcg
      781 tgccgggtgg tctcgttcag gtagatgtcg acgcagacca tcgcctccag gtcggccagc
      841 gccgcgtcca ggcgcggccc gccgggcgcg ctgcgcgccg ggttgcccgc cacggtgatg
      901 agcccgcgga tctggccgtc gccgggcgtg tcgatctcct cggccaggca ggcgctgggc
      961 agctcgccgt agacctcgcc caggccgctg acgcggctgc gccagcggtg cagctccagc
     1021 ggccgggcgg ggcccggcag cgcgttgcgg tgcccggccg cggccagcgg gaacatcggg
     1081 ccgccctcgt ggtccagcag gccacacagc acgttcacca cttcgatcag ccagctcgtc
     1141 agcgtgccga agcgctgcgt ggtggtgccg atgcgcccgt acaggcagcc gcgctcggcc
     1201 gcggccagct cgcgggccag cgtgcggatg tcctcggccg ccacgccgca gcgcgccgcg
     1261 acggtctccg gcgcgtacgg cgcggcaacc gcccggacgt cgtcgatgcc gcggaccagg
     1321 ccgtccaggc ggcgcaggcg caccaggtct tcggcgaaca gcgtgtgcac cacgccgagc
     1381 agcagcaggg cgtcggtgcc cggcaggatc gccaggtggc ggtcggcggc ccgtgccgtg
     1441 cgggaccggc gcgggtcgat cacgaccagc cgcccgccgc gcgccttgag cgcgcgcatg
     1501 cggccgggca ggtccggcac ggtccacagc gagccgttcg aggtgaacgg gtcggcgccc
     1561 atgacgacca ggtagtcgca gcggtcgatg tcgggcaccg ggatggagaa cttgtcgccg
     1621 tacatgagcc cgacgctgac gtgcttgggc atctggtcca gcgtcgatgc gctgaagatg
     1681 ctgcgcgtgc cggcggcgcg cgccaggacc gggttgtaca gcgggccgga gacgttgtgg
     1741 gtggtggggt tgccgaagta gatggccagc gcctcgcgcc cgtggcgctc gatcagccgg
     1801 ttcagggcgt cgtcggccgc ctcgaacgcc tcgtcccagc cggccggctc caggcgcccg
     1861 ccgcggcgga ccagcggctc gcgcagccgg tcggggtcgt cgtgcagttc gccgatcgcc
     1921 acgcccttgg ggcacaggta gccgcgcgag aacacgtcct cgtcgtcgcc gtgcaccgcg
     1981 gtgacgcggt cgccctccaa ggtcagcgac agcccgcacg tcgcctcgca gaacgggcac
     2041 acccgcagcg ccgtccgtgt ctccgcccca gcgcccatgc cagctcccat ggggggccac
     2101 ctcccgcgtc gtccccgttc ccccgcgcac ggtagcagcc ggcggccggc gacggaacga
     2161 aaatgccgct cgcgttccat gcccgcgcgc cgctggtcta ggccgtaggg atcacaggta
     2221 ggggcgacac tgggtccgct gtgtgaggcg gtcacggtgc tcgactctct ggaccttcca
     2281 cagttcagag gagagcaccg tgaccgctac agacgttatc taccagcggc gtgtcgccgc
     2341 gatt
//
LOCUS       DATMKC010000080        12567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_182978, whole genome shotgun sequence.
ACCESSION   DATMKC010000080 DATMKC010000000
VERSION     DATMKC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12567
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182978"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VNU01_02275"
     CDS             <1..331
                     /locus_tag="VNU01_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31479.1"
                     /translation="PADDEICVKTSTRRTFNTLVFQVEVQGYLASDTSALANGVGAWT
                     GLTTGTTTRGAVQQVTIPSGTLITSTLGQATFTLTVARTSALPTAPDVSNFTVPVRAV
                     ANSGDCG"
     gene            328..858
                     /locus_tag="VNU01_02280"
     CDS             328..858
                     /locus_tag="VNU01_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWH31480.1"
                     /translation="MSARRDERGFTVVEVLVTMSITVVVGALVVTSLIQGSRSTVAAQ
                     DRSEGIAELQLALERLSRELRAADPLTAAEADSVELEITRDGQVRRIAYAVTGRELVE
                     SRTVGAATTSTVLAQEVDVLALTYYDDDGDVIAAPVPTAGLDDVHRAVIRIERFIPGS
                     GEPIEVQTTVYLRNST"
     gene            855..2147
                     /locus_tag="VNU01_02285"
     CDS             855..2147
                     /locus_tag="VNU01_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31481.1"
                     /translation="MMATVRTRALTAGRARLDDERGSLLLALLVAILAMTMAVGALAS
                     ARVADRAVRRDVSYATVVQAADAGVQHALSRLNQQQVLASQVTPVPIGDHEFTYAVTP
                     VVTSSGSLAYEVQATGTAPDGEQRVVVARLEQESRLPYALFTNLSIDFRGANLVDSYS
                     SVAVCNSLPGCAWDAPYDGLGMVGSNNSVLYPGNADADKTILFDYDANPDPLRCRENR
                     DPCDPPADTEDDRLDLTTADQLAFIDDMTADCDARVVEGWHDWTSSDSPASPAVLTPP
                     ATPLYDFLCVDDLVFDEDTVLAPIGGSLDARPVVMVVRGAISIGDPANNSAGAEINCT
                     GTCQQDTSVQYPRRLQIYSATAGTVTINPQTGFAGVVYAPRRTCQTGASANVEYFGAL
                     VCDGISQVGGWELHYDRALAGIGTGRYVVGEWREELAP"
     gene            2144..3838
                     /locus_tag="VNU01_02290"
     CDS             2144..3838
                     /locus_tag="VNU01_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWH31482.1"
                     /translation="MTPIPKRRGAGLGTILIEAGLVSESQLAAAQDEQAQSGRSLASV
                     LVDGGYVEERDLVRTLAEHLGLEFVELGDRQVDTEAASLVPEQLARRYNVLPLGFAED
                     GALLVAMSDPSNVLAHDDLRTVTDHEIRPLMATRTDISAAIDRFAGMADAVDDVVGDL
                     GGDDDEVPDLSQIREIVDDAPIVKFVNALIAKAVTDRASDIHIEPQEREIRVRYRVDG
                     VLHDVTSQSRRIHSGVVSRLKIMADLDIAERRIPQDGRISLRTGGKQIDLRVSTLPTV
                     YGEKVVMRILDKTSILLELEDLGFMESNLARYRSSFSKPYGMILVTGPTGSGKSTTLY
                     ATLNIISKPSVNVLTVEDPVEYRLPGISQVQINPKAGLTFPTALRSILRQDPDVVLVG
                     EMRDHETAQIGMEAALTGHLVLSTLHTNDAPSAVTRLTEMRIDPFLVASAVDCVLAQR
                     LARRLCPQCKEPYTPTMAELESAGIPLGEHDRLPTLFRPVGCGHCARTGYRGRLAVHE
                     IMSVTEEIERMIVEKASTDDIAKMAKTQGMHSLREDGLAKVLLGQTTLEEIGRVIV"
     gene            3835..5400
                     /locus_tag="VNU01_02295"
     CDS             3835..5400
                     /locus_tag="VNU01_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWH31483.1"
                     /translation="MSSGLAEVLVAQGVVDEAAVAQAVTLAGERGVPLGKVLVELGAA
                     SEGDIVGAVARQLGLPFADLAPGAVDPSAAALLPRSVARELMALPVEFGADGGLVVAI
                     ADPANRGAAERVSALTGMACTVALAVKGELMEAIEHLADPDGDAGGGATPAPGADTPK
                     LAFEEAGLFDLAAALAELVESGGSDLHLTAGLPPTMRVNGELAPMGGYPVLTPTELQK
                     AIYGILTQRQREQFENELELDLSHSVSGLARFRVNVFRQRDAIGAVIRVIPFGVKAFD
                     QLGLPSQVANFAYLPRGFVLVTGPTGSGKSTTLASLIDIINRERACHIMTVEDPIEFL
                     HEHKRSVVNQRELGADTRSFAQALKHVLRQDPDVILVGEMRDLETIQVALTAAETGHL
                     VFGTLHTQDAPQTVDRVIDVFPPHQQEQIRVMLSGALQGVLTQQLLKTADGKGRVLAA
                     EVMVATPAIRNLIREGKTHQMYSMLQSGKQHGMVTMDQSLADLVKAGKVRYEDALERC
                     TSVPDFNRLCGRA"
     gene            5400..6623
                     /locus_tag="VNU01_02300"
     CDS             5400..6623
                     /locus_tag="VNU01_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWH31484.1"
                     /translation="MSTATTYRYTVRDATGKTRSGTLEAADKKLVASRLREMGFAPVS
                     ITPVETGGLQREISIPGFGPKVDLEALSVFSRQFATMIGSGVTLIRALNILAEQTENK
                     KLAEIIGEMRSEIEGGRSLSDSLRKHEDFPPLYVAMVKAGETAGNLDQVLLRIADTLE
                     KDLALRRKIKAALTYPVVVLILAVFLTGVMLTFIVPTFVGMFESLGGELPLPTKMLLL
                     MSAALRKFWWLLMLLPPLAWRGFLRARKVPEVRRRLDVVKLKLPVFGDLFHKVALSRF
                     SRNFGTLIRSGVPILTALEITADTVNNAVMGDAVNDVKLSVKEGESIAAPLGRHEIFP
                     PMVVQMIAVGEETGALDTMLGKIADFYDSEVESTTESLTAMLEPVMIAILGGLVGGMV
                     ISLYMPMFRIFDLIQ"
     regulatory      6746..6833
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6905..7294
                     /locus_tag="VNU01_02305"
     CDS             6905..7294
                     /locus_tag="VNU01_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HWH31485.1"
                     /translation="MTIQNLREREDGFTLIELLVVIIIIGILAAIAIPTFLNQREKGW
                     DSAAKSDLKSAAIAQESYFTENNGYAGTTALLNDEGFNGSNDVDLTFIGTATGDSDYC
                     MSAAHESSSTTWYLDSEDGVITTAACS"
     gene            7537..7749
                     /locus_tag="VNU01_02310"
     CDS             7537..7749
                     /locus_tag="VNU01_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH31486.1"
                     /translation="MAEFTGFGDDILLTVAEVAQHMRVSNMTVYRLIKSGQLPALRVG
                     KNFRIRGRDLSAYLEASRTGVEESQG"
     gene            7753..8820
                     /gene="pilM"
                     /locus_tag="VNU01_02315"
     CDS             7753..8820
                     /gene="pilM"
                     /locus_tag="VNU01_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HWH31487.1"
                     /translation="MPAAIGLDIGSSTVRAVQISPSGRGHATLERVAQAPLAPGAVHD
                     GEVVDPELVAMALRELWAAGGFKGRRVALGVSNQQVVVRQVDLPYLPDRELRQSLQFQ
                     VDEAIPIPVEQAILDYHPLETLTAEDGRQISRILLVAAQREMVQRLVEAVSRARLEPV
                     LVDLDAFAMLRSLAPESSRGLLDEPDGELLIDLGSVVTNLVVHSQGQPRFVRILLMGG
                     DAITDELEHTLGVDRDEAERLKAETGLLEPMELLEAHQAARIISERAGRFLDEVRGSV
                     DYYAAQPDAVPVRRAVLTGGASRLRGLAERLEDALGMPVERGHPMQELRIGTVGLSHA
                     ELVDLQPDLAVAIGLALGAAE"
     gene            8817..9452
                     /locus_tag="VNU01_02320"
     CDS             8817..9452
                     /locus_tag="VNU01_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31488.1"
                     /translation="MSVKVNLLPREVEERARERRVGLLSLTAVGVWVLLLALLYLQSV
                     MAVNNARDERDAEQAELERLQAQAERLAPYRELARRYDTHNQLLASAMAPQVSWSGVL
                     NDLALVFPANSSLLTLSASLGVPEGEVGGEVPLIGSLQFNGYSVERYAPGVESVLLAL
                     GDAPAFQALYLSSATRGLIGTTQVTNFTGSAELTERVYTGRFAQGLPEGER"
     gene            9449..10099
                     /gene="pilO"
                     /locus_tag="VNU01_02325"
     CDS             9449..10099
                     /gene="pilO"
                     /locus_tag="VNU01_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HWH31489.1"
                     /translation="MSRRAPLFAVLAALLLAVLAWMFLITPERDERALLLEETEAIRT
                     QQQRQRLEIARLEEIRDNELEVRAAIARLEQYIPHGPAQPSTIRQIQLAADASGVEIT
                     ALAFADPQVVVDAPSPADPGSIMAQIPVTLTIEGAYFQTTDFMRRVETELARAVMVDS
                     VTATEASAPAGFPTLSVSMTARIFTVVPVSAVPGAELLAPPEDEAAEGAEEEGSAA"
     gene            10096..10755
                     /locus_tag="VNU01_02330"
     CDS             10096..10755
                     /locus_tag="VNU01_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31490.1"
                     /translation="MSDVERPAVSRNLLIGLGAIALLAVLWLLVLGPMLAGDEEGGDD
                     VVVVPPREARSPDAPLPGEDAPDADGGLLYPETTEVFAARDPFAQLVRTGPDGGGGDD
                     DDGGTGAGTDGGTGTGDGTSGGTGDGTSGGTGDGTSPERPSNGSSARVGGTTVKLVDV
                     YTEDDQERALVTVNGRSYDVAEGESFGDRFRLLDLSGECGTFLFGDSRFVLCEGDEIR
                     K"
     gene            10752..12035
                     /locus_tag="VNU01_02335"
     CDS             10752..12035
                     /locus_tag="VNU01_02335"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HWH31491.1"
                     /translation="MTRVHDRGPLRPRADDLGEQIERSLRESRPVRLALLVGCLGLLA
                     QAAVTLLSLPLGDDLVVYWKGGWAVLHGLPLFGEHFTAYAGIDNALVFTYPPFAALLF
                     VPLALVPPEAAMVAWTVASLAALAALAADGFRPWWRGRPGPAVVFVLLAVLAVSFTFQ
                     PVSNGLLLGQIGFMLTLLCYADVVVLHGRRPAGVAIGLATAIKLTPGLFLVHFAVTRQ
                     WRALRNAVLTIAACWGVAFVALPAATVTYFRHLMLDTTRMGSVVQPINQSLNATVQRF
                     GLPQPRLWWLLLAAVVTAAGLDRARRAHDLGERRRAAVLVAMAMLLVSPVSWTHHAIW
                     LIPLFGLLLTDRRPGPRLLGVVAVLLAAPTRQLLGLPGLGDGIMVEGYVLLYLAALVW
                     LPVDSAAPLPAAAPAQNQGQAAGPRAAVPELATRS"
     gene            12032..>12567
                     /locus_tag="VNU01_02340"
     CDS             12032..>12567
                     /locus_tag="VNU01_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31492.1"
                     /translation="MIAGLADRYPWAAAAVFGAAGFALGDHAHEPADFTLFAEAGGHL
                     LRGDVPAVLGDAAVQAGPLLLALMGLLDGATAWLGGAQRHAVLLALCLTVSVSLLAAV
                     RALLPQPGAARGRALLAAGVAALAFGVVTLNEAHPTHAVVPLLWLLAALACRRDRPAA
                     AGVLLGLAAGLDSWGVLGA"
BASE COUNT         1751 a         4486 c         4542 g         1788 t
ORIGIN      
        1 ccctgccgac gacgagatct gcgtcaagac gtccactcgc cgcaccttca acaccttggt
       61 cttccaggtg gaggtgcagg gctacctcgc ctcggacacc tccgcgctcg ccaacggtgt
      121 cggcgcctgg acgggcctca ccacgggcac gacgacgcgc ggcgccgtgc agcaggtcac
      181 cattccgtcg gggacgctga tcacctcgac gctcggccag gccacgttca cgctcacggt
      241 cgcccgcacg agcgcgctcc cgaccgcgcc ggacgtgagc aatttcacgg tcccggtgcg
      301 ggccgtcgcc aactcggggg actgcggatg agcgcccgcc gggacgagcg cggcttcacg
      361 gtggtggagg tcctcgtgac gatgtccatc accgtcgtgg tcggcgccct ggtcgtgacg
      421 tcgctgatcc agggctcccg ttccaccgtg gcggcccagg accgcagcga gggcatcgcg
      481 gagttgcagc tggccctgga gcggctgagc cgcgagctgc gcgcggcgga cccgctcacg
      541 gcggcggagg ccgacagcgt cgagctggag atcacgcgcg acgggcaggt ccgccggatc
      601 gcctacgccg tcacggggcg ggagctcgtg gagagccgca ccgtgggggc cgccaccacg
      661 tcgaccgtgc tggcgcagga ggtcgacgtg ctggcgctca cctactacga cgacgacggc
      721 gacgtgatcg ccgcaccggt gccgaccgcc ggcctggacg acgtgcaccg cgccgtgatc
      781 cgcatcgagc gctttatccc gggctccggt gagccgattg aagtgcagac gaccgtgtac
      841 ctgaggaatt cgacgtgatg gccaccgtgc gcacccgcgc gctcaccgca ggccgcgccc
      901 gtctcgacga cgagcgcggc tcgctcctgc tcgcgctgct ggtggcgatc ctggcgatga
      961 ccatggcggt cggggcgctc gcgtccgcgc gggtggccga ccgggccgtc cgccgcgacg
     1021 tgtcctacgc cacggtcgtg caggcggccg acgccggggt gcagcacgcg ctgtcccggc
     1081 tcaaccagca gcaggtcctg gccagccagg tcaccccggt ccccatcggg gaccacgagt
     1141 tcacctacgc cgtgacgccg gtggtcacgt cctcagggag cctggcctac gaggtgcagg
     1201 ccaccggcac ggctccggat ggcgagcagc gcgtcgtggt cgcgcgcctg gagcaggagt
     1261 cgcgcctgcc ctacgcgctg ttcaccaacc tgagcatcga tttccgcggc gccaacctcg
     1321 tcgactccta ctcgtcggtg gcggtgtgca actcgctgcc ggggtgcgcc tgggacgcgc
     1381 cctacgacgg gctcggcatg gtgggcagca acaactccgt gctgtacccg ggcaacgcgg
     1441 acgcggacaa gaccatcctg ttcgactacg acgccaaccc ggacccgctg cgctgccggg
     1501 agaaccgcga tccgtgcgat ccgcccgccg acaccgagga cgaccggctg gacctgacca
     1561 ccgccgacca gctcgcgttc atcgacgaca tgacggccga ctgcgacgcg cgcgtcgtcg
     1621 agggctggca cgactggacc tcgtcggact cgccggcgag cccggccgtg ctcacgccgc
     1681 cggccactcc cctctacgac ttcctctgcg tcgacgacct ggtcttcgac gaggacacgg
     1741 tgctcgcccc catcggcggc tcgctggacg ccaggccggt ggtcatggtg gtccgcggcg
     1801 cgatcagcat cggtgacccg gccaacaaca gcgcgggggc ggagatcaac tgcacgggca
     1861 cctgccagca ggacacgtcc gtgcagtacc cgcggcggct gcagatctac agcgccaccg
     1921 cgggcaccgt gacgatcaac ccgcagaccg gcttcgccgg ggtggtctac gccccccgcc
     1981 ggacctgcca gaccggcgcc tcggcgaacg tggagtactt cggcgcgctg gtgtgcgacg
     2041 gcatcagcca ggtcggcggc tgggagctgc actacgaccg cgcgctcgcg ggcatcggca
     2101 ccggccgcta cgtggtgggc gagtggcgcg aggagctggc gccgtgacgc cgatccccaa
     2161 gcgccgtggc gccggcctcg gcacgatcct gatcgaggcc ggcctggtgt ccgagtccca
     2221 gctcgccgcc gcccaggacg agcaggccca gtccggtcgg tcgctggcca gcgtgctggt
     2281 cgacggcggc tacgtcgagg agcgcgacct ggtgcgcacc ctggccgagc acctgggcct
     2341 ggagttcgtg gagctgggcg accgccaggt cgacaccgag gccgccagcc tggtgccgga
     2401 gcagctggcg cgccgctaca acgtgctgcc gctgggcttc gccgaggacg gcgcgctgct
     2461 ggtcgccatg agcgatccgt ccaacgtgct ggcgcacgac gacctgcgca ccgtcaccga
     2521 ccatgagatc cggccgctga tggccacgcg caccgacatc agcgcggcga tcgaccgctt
     2581 cgcgggcatg gccgacgcgg tggacgacgt ggtcggcgac ctcggcggcg acgacgacga
     2641 ggtcccggac ctgtcccaga tccgcgagat cgtcgacgac gcgccgatcg tgaagttcgt
     2701 caacgccctg atcgccaagg cggtcaccga ccgcgcgtcg gacatccaca tcgagccgca
     2761 ggagcgcgag atccgcgtgc gctaccgcgt ggacggcgtg ctgcacgacg tcaccagcca
     2821 gtcgcgccgc atccactccg gcgtggtcag ccgcctgaag atcatggcgg acctggacat
     2881 cgccgagcgg cgcatcccgc aggacgggcg catcagcctg cgcaccggcg gcaagcagat
     2941 cgacctgcgc gtgtcgaccc tgccgaccgt gtacggcgag aaggtcgtca tgcgcatcct
     3001 ggacaagacc tccatcctgc tggagctgga ggacctgggc ttcatggagt ccaacctggc
     3061 gcgctaccgc tccagcttca gcaagccgta cgggatgatt ctggtcaccg ggcccaccgg
     3121 ctcgggcaag tccaccaccc tgtacgcgac gctgaacatc atctccaagc ccagcgtgaa
     3181 cgtgctcacg gtggaggacc ccgtcgagta ccggctgccc ggcatcagcc aggtgcagat
     3241 caaccccaag gcgggcctga cgttcccgac ggcgctgcgc tccatcctgc gccaggaccc
     3301 cgacgtggtc ctggtgggcg agatgcgcga ccacgagacc gcccagatcg gcatggaggc
     3361 ggcgctcacg ggccacctgg tgctgtcgac gctgcacacc aacgacgcgc cgtccgcggt
     3421 cacgcgcctg acggagatgc gcatcgaccc gttcctggtg gcgtccgcgg tggactgcgt
     3481 gctggcccag cgcctggcgc ggcgactgtg cccgcagtgc aaggagccgt acacgcccac
     3541 gatggccgag ctggagtccg cgggcatccc cctgggcgag cacgaccgcc tgcccacgct
     3601 gttccggccg gtcggctgcg gccactgcgc gcgcaccggt taccgcgggc ggctggcggt
     3661 ccacgagatc atgagcgtca ccgaggagat cgagcgcatg atcgtggaga aggcgtccac
     3721 ggacgacatc gccaagatgg ccaagacgca aggcatgcac agcctccgcg aggacgggct
     3781 ggcaaaggtg ctcctgggtc agacgaccct ggaggagatc ggtcgggtga tcgtgtgagc
     3841 agtggtctcg cggaagtgct ggtggcgcag ggcgtcgtcg acgaagcggc ggtggcgcag
     3901 gctgtcacgc tcgccggcga gcgcggcgtg ccgctgggca aggtgctcgt ggaactgggg
     3961 gcggccagcg agggcgacat cgtcggcgcc gtcgcccgcc agctcggctt gccgttcgcc
     4021 gacctggcgc ccggtgccgt ggacccctcg gcggcggcgc tgctgccgcg ctcggtggcg
     4081 cgcgagctca tggcgctgcc cgtggagttc ggcgcggacg gcgggctcgt cgtggcgatc
     4141 gcggaccccg ccaaccgcgg cgcggccgag cgcgtgagcg cgctcacggg catggcgtgc
     4201 accgtggcgc tggccgtgaa gggagagctg atggaggcca tcgagcacct ggcggacccc
     4261 gacggggacg ccggcggcgg ggcgacgccg gctcccggcg ccgacacgcc caagctggcg
     4321 ttcgaggagg cggggctgtt cgacctcgcc gccgcgctgg ccgagctcgt cgagtccggc
     4381 ggcagcgacc tgcacctgac ggccggcctg ccgccgacga tgcgcgtcaa cggcgagctc
     4441 gcgcccatgg gcggctaccc ggtgctgacg cccacggagc tgcagaaggc gatctacggc
     4501 atcctcacgc agcgccagcg cgagcagttc gagaacgagc tggagctgga cctgtcccac
     4561 tcggtctccg gcctggcccg cttccgcgtc aacgtgttcc gccagcgcga cgccatcggc
     4621 gccgtgatcc gcgtgatccc gttcggcgtc aaggcgttcg accagctcgg cctgccgtcg
     4681 caggtggcca acttcgccta cctgccgcgc ggcttcgtgc tggtgaccgg ccccaccggc
     4741 tcgggcaagt ccaccacgct ggcctcgctg atcgacatca tcaaccgcga gcgcgcgtgc
     4801 cacatcatga ccgtggagga ccccatcgag ttcctccacg agcacaagcg cagcgtggtc
     4861 aaccagcgcg agctgggcgc ggacacgcgc agcttcgccc aggcgctcaa gcacgtgctg
     4921 cgccaggacc ccgacgtgat cctggtgggc gagatgcgcg acctggagac catccaggtg
     4981 gcgctgaccg ccgcggagac cggccacctg gtgttcggca cgctgcacac ccaggacgcg
     5041 ccgcagaccg tggaccgcgt catcgacgtg ttcccgccgc accagcagga gcagatccgc
     5101 gtcatgctct ccggcgccct ccagggcgtg ctcacgcagc agctgctgaa gaccgccgac
     5161 ggcaagggcc gcgtcctcgc cgcggaggtc atggtggcca cgccggccat ccgcaacctc
     5221 atccgcgagg gcaagaccca ccagatgtac tccatgctgc agtccggcaa gcagcacggc
     5281 atggtgacca tggaccagtc gctggccgac ctggtcaagg ccggcaaggt gcgctacgag
     5341 gacgccctgg agcgctgcac gtcggtgccg gacttcaacc gcctctgcgg gagggcgtga
     5401 tgtcgaccgc cacgacgtac cgctacacgg tccgcgacgc cacgggcaag acccgcagcg
     5461 gcacgctgga ggcggccgac aagaagctgg tcgcctcgcg cctgcgcgag atgggcttcg
     5521 cccccgtgtc gatcaccccg gtggagacgg gcgggctcca gcgggagatc tccatcccgg
     5581 gcttcggtcc gaaggtggac ctggaggcgc tgtcggtctt ctcgcggcag ttcgcgacca
     5641 tgatcggctc cggcgtcacg ctcatccgcg cgctcaacat cctggccgag cagacggaga
     5701 acaagaagct ggccgagatc atcggcgaga tgcggtcgga gatcgaaggc ggccgctcgc
     5761 tgtcggacag cctgcgcaag cacgaggact tcccaccgct gtacgtcgcc atggtcaagg
     5821 ccggcgagac ggcgggcaac ctggaccagg tgctgctgcg catcgccgac acgctggaga
     5881 aggacctggc gctgcggcgc aagatcaagg ccgcgctgac ctacccggtg gtcgtgctga
     5941 tcctggcggt gttcctcacc ggcgtgatgc tcacgttcat cgtgccgacg ttcgtcggca
     6001 tgttcgagtc gctgggcggg gaattgccgc tgcccaccaa gatgctgctg ctcatgtcgg
     6061 ccgccctgcg caagttctgg tggctgctca tgctgctgcc gccgctggcc tggcgggggt
     6121 tcctgcgcgc gcgcaaggtg ccggaggtgc gccgtcgcct ggacgtggtg aagctcaagc
     6181 tgccggtgtt cggcgacctg ttccacaagg tggcgctgtc gcgcttctcg cggaacttcg
     6241 gcacgctgat ccgcagcggc gtgccgatcc tcaccgccct ggagatcacc gccgacacgg
     6301 tgaacaacgc cgtcatgggc gacgcggtga acgacgtgaa actgtccgtc aaagagggcg
     6361 agagcatcgc cgcgccgctg gggcgccacg agatcttccc cccgatggtg gtgcagatga
     6421 tcgccgtggg ggaggagacc ggcgcgctgg acaccatgct gggcaagatc gccgacttct
     6481 atgacagcga ggtggagtcc accaccgagt cgctgacggc catgctggag ccggtgatga
     6541 tcgcgatcct gggcggcctg gtcggcggca tggtgatctc gctgtacatg ccgatgttcc
     6601 gcatcttcga cctcatccag tagcgccggg aattcgctca agccgcggct tgcgggcgcc
     6661 gatatgtgag gcaggcctgc cctcgatcat cgtcatcctc ttttcgccgc gggtgggccc
     6721 agtcaggcaa gtaccgcaag gagcggcgca gaaacggcaa acccgtcgtg aggcggggac
     6781 gcaaagccac gggaccgcat ggccccatgc cagtggtcag ccgggttgcc tcgcgcgaca
     6841 cgtcacgtgc gcgttggggg tcgggcaccg cgccttgcct gaacgcacct caaggaggcg
     6901 caccgtgacc atccagaacc tgcgcgagcg cgaggacggc ttcaccctca tcgagctgct
     6961 cgtcgtcatc atcatcatcg gcatcctggc ggccatcgcc atcccgacgt tcctgaacca
     7021 gcgcgagaag ggctgggact cggccgcgaa gagcgacctc aagagcgccg cgatcgccca
     7081 ggagtcctac ttcaccgaga acaacggcta cgcgggcacc accgcgctgc tcaacgacga
     7141 gggcttcaac ggctccaacg acgtggacct gaccttcatc ggcaccgcga cgggcgactc
     7201 ggactactgc atgagcgcgg cgcacgagag ctcgtccacg acgtggtacc tggacagcga
     7261 ggacggggtc atcaccacgg ccgcgtgctc gtaagccgcc gcggcggcaa cgggagttcg
     7321 gtggggcggt ccttcggggc cgccccaccg ccgtttcccc gtcgcatcct ccccgtaagg
     7381 tttcgccccc tgctgccgat gtgaatgttg acgaagaatg ttgctgctgt tcaccatgtg
     7441 gcgaacgggg cgcatcgcga ctaccgtgca gggcttgcag cgggcgggac gagaagggca
     7501 caccgggatg tgcccggatg cggagaggac cgacagatgg cggagttcac gggttttggc
     7561 gacgacatcc tgttgactgt tgccgaagtg gcacagcaca tgagggtgtc gaacatgacc
     7621 gtgtaccgcc tgatcaagtc cggacagctg ccggccctgc gggtcggcaa gaacttccgc
     7681 atccgcggcc gagacctcag cgcttacctg gaggcctcgc gcacgggggt ggaggaatcc
     7741 caggggtagt ccatgcccgc cgccatcgga ctcgacatcg ggtccagtac ggtccgcgcc
     7801 gtacagatct cgccgtccgg gcgtggccac gccacgctcg aacgcgtcgc ccaggcgccg
     7861 ctggcgcccg gggcggtgca tgacggcgag gtcgtcgacc cggagctggt cgccatggcc
     7921 ctgcgcgagc tgtgggccgc gggcggcttc aagggccggc gggtcgccct gggggtgtcc
     7981 aaccagcagg tcgtcgtgcg ccaggtcgac ctgccgtacc tgccggacag ggagttgcgg
     8041 cagtccctgc aattccaggt cgacgaggcc atcccgatcc cggtcgagca ggccatcctg
     8101 gactaccacc cgctggagac gctgaccgcc gaggacggcc gccagatcag ccggatcctg
     8161 ctggtggccg cgcagcggga gatggtgcag cgcctggtcg aggcggtgtc acgggcgcgc
     8221 ctggagccgg tgctggtcga cctggacgcc ttcgccatgc tgcgcagcct cgcgccggag
     8281 tcctcccgcg ggctgctgga cgagccggac ggcgagctgc tgatcgacct gggcagcgtg
     8341 gtcaccaacc tggtcgtgca ctcgcagggg cagccgcgct tcgtgcgcat cctgctcatg
     8401 ggcggcgacg cgatcaccga cgagctggag cacaccctgg gcgtggaccg cgacgaggcc
     8461 gagcgcctca aggcggagac cggcctgctg gagccgatgg agctgctgga ggcccatcag
     8521 gccgcacgga tcatctccga gcgcgccggg cgcttcctgg acgaggtgcg cggctcggtc
     8581 gactactacg cggcccagcc cgacgccgtg ccggtgcgcc gcgccgtgct caccggcggc
     8641 gccagccgcc tgcgcggcct ggccgagcgg ctggaggacg cgctgggcat gccggtcgaa
     8701 cgcggccatc ccatgcagga gctgcgcatc ggcacggtgg gcctcagcca cgccgagctg
     8761 gtcgacctgc agcccgacct cgccgtggcg atcggcctgg cgctgggggc ggcggaatga
     8821 gcgtcaaggt caacctgctg ccgcgcgagg tcgaggagcg cgcccgcgag cgccgggtcg
     8881 ggctgctgtc gctcaccgcc gtcggcgtgt gggtgctgct gctcgcgctg ctgtacctgc
     8941 agagcgtgat ggcggtgaac aacgcgcgcg acgagcgcga cgccgagcag gcggagctgg
     9001 agcgcctcca ggcccaggcc gagcgcctgg cgccctatcg cgagctggcc cgccgctacg
     9061 acacgcacaa ccagctcctg gccagcgcga tggccccgca ggtgtcgtgg tcgggcgtgc
     9121 tgaacgacct ggcgctggtg ttcccggcca actcctcgct gctgacgctc agcgcctcgc
     9181 tgggcgtgcc ggagggcgag gtcggcggcg aggtcccgct gatcggctcc ctgcagttca
     9241 acggctactc ggtggagcgc tacgcgccgg gtgtggagtc ggtcctgctg gcgctgggcg
     9301 acgcgcccgc gttccaggcg ctgtacctgt ccagcgccac gcgcggcctc atcggcacca
     9361 cgcaggtcac caacttcacc ggctcggccg agctcaccga acgcgtctac accgggcgct
     9421 tcgcccaggg cctgccggag ggtgagcgat gagccggcgc gcgcccctgt tcgccgtgct
     9481 cgccgcgctg ctgctggccg tgctggcgtg gatgttcctg atcacgccgg agcgcgacga
     9541 gcgcgcgctg ctgctggagg agaccgaggc catccggacc cagcagcagc gccagcgcct
     9601 ggagatcgcc cggctggagg agatccgcga caacgagctc gaggtgcgcg cggccatcgc
     9661 gcggctggag cagtacatcc cgcacgggcc ggcgcagccc tcgacgatcc gccagatcca
     9721 gctcgcggcc gacgcctccg gtgtggagat caccgcgctg gccttcgccg acccccaggt
     9781 ggtcgtcgac gccccgtctc cggccgatcc gggcagcatc atggcgcaga tcccggtgac
     9841 gctgaccatc gagggcgcct acttccagac caccgacttc atgcggcgcg tggagaccga
     9901 gttggcgcgc gccgtcatgg tcgactccgt gaccgccacc gaggcctcgg cgcccgcggg
     9961 gttccccacc ctgtcggtca gcatgaccgc gcgcatcttc accgtggtcc ccgtgtccgc
    10021 cgtgcccggc gccgagctgc tggcgccgcc cgaggacgag gcggccgagg gcgccgagga
    10081 ggaggggagc gcggcatgag cgacgtggag cggccggcgg tgagccgcaa cctcctgatt
    10141 ggcctggggg cgatcgccct gctggcggtg ctgtggctgc tcgtgctggg ccccatgctc
    10201 gccggcgacg aggagggcgg cgacgacgtc gtggtcgtgc cgccccgcga ggcccggtcc
    10261 ccggatgcgc cgctgccggg agaggacgcg cccgacgccg acggcggcct gctgtacccg
    10321 gagaccacgg aggtgttcgc ggcacgcgac ccgttcgcgc agctggtcag gaccgggccc
    10381 gacggcggtg gcggcgacga cgacgatggc ggcaccgggg ccgggaccga cgggggaacg
    10441 ggcacgggcg acggcacgtc cggcggcacc ggcgacggca cgtccggcgg caccggtgac
    10501 ggcacgtcgc ccgagcggcc cagcaacggc tcgtcggccc gcgtgggcgg caccacggtc
    10561 aagctggtcg acgtgtacac cgaagatgac caggagcggg cgctggtcac ggtcaacggg
    10621 cgctcctacg acgtggccga gggcgagtcg ttcggggacc ggttccgcct gctggacctg
    10681 tccggcgagt gcgggacgtt cctgttcggc gacagccgct tcgtcctgtg cgaaggcgac
    10741 gagatcagga agtgacgcgg gtgcacgaca gggggccgct ccgcccacgg gccgacgacc
    10801 tgggggagca gatcgagcgc agcttgcggg agtcgcggcc cgtgcgcctc gcgctgctcg
    10861 tcggctgcct cgggctgctg gcccaggcag cggtcacgct gctgagcctg ccgctgggcg
    10921 acgacctggt cgtctactgg aagggcggct gggcggtcct gcacgggctg cccctgttcg
    10981 gcgagcactt caccgcctac gccggcatcg acaacgcgct ggtgttcacg tacccgccgt
    11041 tcgcggcgct gctgttcgtg ccgctggcgc tggtgccgcc cgaggcggcg atggtggcct
    11101 ggacggtcgc gtcgctggcc gccctggccg cgctggcggc cgatggcttc cggccctggt
    11161 ggcgcgggcg ccccggcccc gccgtggtgt tcgtgctgct ggccgtgctg gccgtgtcgt
    11221 tcacgttcca gcccgtctcc aacgggctgc tgctcggcca gatcggcttc atgctcacgc
    11281 tgctgtgcta cgccgacgtg gtggtcctcc acggccgccg gccggcgggt gtcgccatcg
    11341 gcctggccac ggcgatcaag ctcaccccgg ggctgttcct ggtgcacttc gccgtgacgc
    11401 ggcagtggcg ggcgctgcgc aacgccgtgc tgaccatcgc cgcctgctgg ggcgtcgcgt
    11461 tcgtggcgct gcccgcagcc acggtcacct acttccgcca cctcatgctg gacaccacgc
    11521 gcatgggctc ggtggtgcag ccgatcaacc agtcgctgaa cgccacggtg cagcgcttcg
    11581 gcctgccgca gccgcgcctg tggtggctgc tgctggccgc cgtggtgacc gcggccgggc
    11641 tcgaccgcgc ccgccgcgcg cacgacctgg gcgagcgccg ccgcgccgcc gtgctggtcg
    11701 cgatggccat gctgctggtg tcgccggtgt cctggacgca ccacgcgatc tggctgatcc
    11761 cgctgttcgg cctgctgctc accgaccggc ggcccggccc gcgcctgctg ggcgtggtgg
    11821 cggtgctgct cgccgcgccc acgcggcagc tcctgggcct gcccggcctc ggcgacggga
    11881 tcatggtcga gggctacgtc ctgctctacc tcgcggcgct ggtgtggctg ccggtggaca
    11941 gcgccgcccc gctgcccgcc gcggccccgg ctcagaacca gggccaggcg gcggggccgc
    12001 gcgccgccgt gccggagctc gccacgcgct cgtgatcgcg ggcctggcgg accgctaccc
    12061 gtgggcggcc gcggccgtct tcggcgctgc gggcttcgcg ctcggggacc acgcgcacga
    12121 gcccgcggac ttcacgctgt tcgccgaggc gggcgggcac ctgctgcgcg gcgacgtccc
    12181 ggcggtgctc ggcgacgccg ccgtgcaggc cggaccgctg ctgctcgcgc tcatgggcct
    12241 gctggacggg gccaccgcgt ggctcggcgg cgcgcagcgt cacgcggtgc tgctggccct
    12301 gtgcctcacc gtgtcggtgt cgctgctggc ggccgtgcgc gcgctgctgc cgcaacccgg
    12361 tgccgcgcgc ggccgggcgc tgctcgccgc cggcgtggcg gccctcgcgt tcggcgtcgt
    12421 gacgctgaac gaggcgcatc ccacgcacgc cgtggtcccg ctgctgtggc tgctcgcggc
    12481 gctggcgtgc cggcgcgacc ggccggccgc ggccggggtg ctgctcgggc tcgccgcggg
    12541 gctggacagc tggggcgtgc tgggggc
//
LOCUS       DATMKC010000081         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_66604, whole genome shotgun sequence.
ACCESSION   DATMKC010000081 DATMKC010000000
VERSION     DATMKC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66604"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VNU01_02345"
     CDS             <1..448
                     /locus_tag="VNU01_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWH31493.1"
                     /translation="LPTTYWTPGPTAGPGGDLDHGMDFGEDDDLVWSLYQQGRARLKD
                     GQPSAAAEVLELAVEREPEKASLREALGRAYFASARLERARQEFERALDLNPSDDYAH
                     FGLGRCLERQGRLAAAAKHLKLAVAMRDRDDYRTALGRVLARLGTG"
     gene            complement(455..1240)
                     /locus_tag="VNU01_02350"
     CDS             complement(455..1240)
                     /locus_tag="VNU01_02350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWH31494.1"
                     /translation="MLTPAPVVAPMVIPLGDHNPTRIRPWATWALIALNLWAFIGVQP
                     WDASECAQQAFYLRWAAVPAELTRGEPLDQAEVAAASDAVCQVQAMPGKPVYLTILTS
                     MFLHAGWLHLLGNMLYLWIFGNNIEDRLGHVRFLLFYLAAGLVATVAFVAPNADNIST
                     LVGASGAIAGVLGAYLVLFPRAHVTVAVPFLLFFITRLPAFLVLGLWFLLQVQGLREP
                     LLEGGGVAYLAHAGGFVFGMAAALLLRSGSGSGGGRPARSRRG"
     gene            complement(1251..>2188)
                     /locus_tag="VNU01_02355"
     CDS             complement(1251..>2188)
                     /locus_tag="VNU01_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH31495.1"
                     /translation="GAAEELRRPDGALLRAVVSGAGHAAGAPTFVLTHGLACDHRLWA
                     YQARLLGEVGRVVTWDLRGHGDSTLGDAAPARALRPDVHAADLAAVVERLATGPVFLV
                     GHSLGGLATLLALRDNEALRARVAGAALVATPTAHVAGGTRGGSAPSAVEAAAVRMLL
                     RWLVGDPVVQRLFLDPGTARARGYAALRVGGFGPRPSPAHVEALRAQIVATPAAVRRA
                     TLAGMERVDIRGTLGAVTTPTLVFVGGRDRLVDPRQAVRLAAELPNARPVVFSSAGHA
                     VVLERHAAITRRIALLAAEILSGRGDLDMVASAAR"
BASE COUNT          319 a          813 c          832 g          224 t
ORIGIN      
        1 gttgccgacg acatactgga cacccgggcc gaccgccggc cccggaggcg acctggacca
       61 cggcatggac ttcggtgagg acgacgacct ggtctggtcc ctgtaccagc agggccgggc
      121 acggctgaag gacggccagc cgtccgccgc ggccgaggtc ctggaactgg cggtggagcg
      181 cgagccggag aaggcgtccc tgcgcgaggc gctgggccgg gcctatttcg cctcggcgcg
      241 cctggagcgc gcccgccagg agttcgagcg ggcgctggac ctcaacccca gcgacgacta
      301 cgcccacttc ggcctgggcc gctgcctgga gcgccagggc aggctggcgg ccgccgccaa
      361 gcacctcaag ctcgccgtgg cgatgcggga ccgcgacgac taccgcacgg ccctgggcag
      421 ggtgctggcg cgcctcggga ctggctgagg ccggtcagcc gcgccggctg cgggccgggc
      481 ggccgccccc cgagcctgag cccgagcgca gcagcagcgc ggccgccatg ccgaacacga
      541 acccgccggc gtgggccagg taggcgacgc cgccaccctc cagcagcggt tcgcgcagtc
      601 cctggacctg gagcaggaac cacagcccca gcaccaggaa cgccggcagc cgggtgatga
      661 agaacagcag gaacggcacc gcgacggtga cgtgcgcgcg cgggaacagc accaggtagg
      721 cgccgagcac gccggcgatc gcgccggacg cgccgaccag ggtggagatg ttgtcggcgt
      781 tgggagccac gaacgcgacc gtcgccacca gcccggccgc caggtagaac agcaggaagc
      841 gcacgtggcc caggcggtcc tcgatgttgt tgccgaagat ccacaggtac agcatgttgc
      901 cgagcaggtg cagccacccg gcgtgcagga acatcgacgt gaggatcgtc aggtacaccg
      961 gcttgccggg catggcctgg acctggcaca ccgcgtcgga ggccgcggcg acttccgcct
     1021 ggtcgagcgg ctcgccgcgg gtgagctccg ccggcaccgc ggcccaccgc aggtagaacg
     1081 cctgttgcgc gcactccgag gcgtcccacg gctgcacgcc gatgaacgcc cagaggttca
     1141 gggcgatcag cgcccaggtg gcccacgggc ggatccgggt ggggttgtgg tccccgagcg
     1201 ggatgaccat gggtgcgacg acgggggcgg gggtgagcat gggcgctctt ctaccgcgcc
     1261 gcggacgcca ccatgtccag gtcgccgcgg ccgctcagga tctccgcggc cagcagcgcg
     1321 atgcgccggg tgatggcggc gtggcgctcc agcaccacag cgtggccggc gctggagaac
     1381 accacaggcc gcgcgttcgg cagctcggcg gcaagccgga ccgcttgccg tgggtccacc
     1441 aggcggtcac ggccgcccac gaacaccagc gtcggagtcg tcaccgcccc cagcgtcccg
     1501 cggatgtcga cgcgctccat gcccgccagc gtggcgcggc ggaccgccgc gggggtcgcc
     1561 acgatctggg cgcgcagcgc ctcgacgtgc gcgggcgagg gccgcgggcc gaatccgccg
     1621 acgcgcagcg cggcgtagcc gcgcgcccgc gcggtgccgg ggtccaggaa cagccgttgc
     1681 accacggggt cgcccaccag ccagcgcagc agcatgcgca cggcggcggc ttcgacggcg
     1741 gaaggcgcgg acccgccgcg cgtgcctccg gccacgtgcg ccgtgggcgt cgccaccagc
     1801 gcggctccgg cgacccgggc ccgcagcgcc tcgttgtcgc gcagcgccag cagcgtggcc
     1861 aggccgccca gcgagtggcc cacgaggaac accggccccg tggccaagcg ctcgaccaca
     1921 gcggccaggt ccgcggcgtg cacgtccggg cgcagggcgc gcgcgggcgc ggcgtcgccg
     1981 agggtggagt ctccgtgccc gcgcaggtcc caggtgacca cgcggccgac ctcgccgagc
     2041 aggcgcgcct ggtaggccca caggcggtgg tcgcaggcca gcccgtgcgt cagcacgaac
     2101 gtgggcgctc cggccgcgtg gccggcacca gagacgaccg ctcgcagcag cgcgccgtcc
     2161 ggccggcgca gttcctccgc ggctccga
//
LOCUS       DATMKC010000082         3936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_280671, whole genome shotgun sequence.
ACCESSION   DATMKC010000082 DATMKC010000000
VERSION     DATMKC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280671"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..512)
                     /gene="moaC"
                     /locus_tag="VNU01_02360"
     CDS             complement(42..512)
                     /gene="moaC"
                     /locus_tag="VNU01_02360"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWH31496.1"
                     /translation="MPGLTHLDEHGEARMVDVAGKQVTQRTATARAVVRMSQGTAALL
                     ASGHLPKGDALAVARVAGIMAAKRTPELIPLCHPIALAGVDVSVTVDPDDGRADVLVT
                     CRAADRTGVEMEALTAAAVAALSVYDMVKGVERGVTIERIELLEKTGGKQDWRR"
     gene            complement(536..1897)
                     /gene="glp"
                     /locus_tag="VNU01_02365"
     CDS             complement(536..1897)
                     /gene="glp"
                     /locus_tag="VNU01_02365"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWH31497.1"
                     /translation="MIFRRRQDDTDPEPPPLFGPEARPAAVPPEAGPAAVPPAAPPHG
                     PREPQPLVPFAEHRKDVLARIRPLEPIELGILDAQGCVLAQEAVAVSDLPPFPNSAMD
                     GYALLAMDAVPGAELALVGESAAGSPARTPVGRGQAVRIMTGAPMPPGADAVVPVEQA
                     EEWPGVVRLAAGAEPGQHVRMAGEDVRHGEVVLSSGRVLAPADIGMLAALGYPRVLVR
                     PQPRVVVISTGDELVEPEEPTGLGQIRDANSYTLTAMARDAGASAYRHQIVRDDERAL
                     TEAFEGALVHADLVVTSGGVSAGRYDLVKVVLARLGDVRFVRVGMRPGMPQAFGLLPR
                     EPDRAVPVFGLPGNPVSAFVSFEMFVRPAIRRLQGRTDTYRPRISAVLDEPLRSPREK
                     VEFVRVRLRREGGAWHARPTGEQGSGILRSVVDADGLAEVPPERTQVAAGERLVVHLL
                     ERP"
     gene            complement(1894..2772)
                     /gene="galU"
                     /locus_tag="VNU01_02370"
     CDS             complement(1894..2772)
                     /gene="galU"
                     /locus_tag="VNU01_02370"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWH31498.1"
                     /translation="MRARKAVIPAAGLGTRFLPATKAQPKEMLPVVDKPAIQYVVEEA
                     VKAGLDDILLVTGRGKRALEDHFDHAVELERQLAAAGKEDLLAAVRAVSQLALVHYIR
                     QGQPLGLGHAVGCARHHVGHEPFAVLLGDDLIGEQERLLSQMVDLSERTGRSVVAVME
                     FGDEALSQYGVIDAEPDPDNPGAYLVRDLVEKPGKANAPSNLCIIGRYVLTPDIFDHI
                     AGTRPGRGGEIQLTDAMRAQCQEEPIRALKFEGLRYDVGAKQDYLRAMVELAAHRADL
                     GPTFRAFLKEFVKGLE"
     gene            complement(2858..>3936)
                     /locus_tag="VNU01_02375"
     CDS             complement(2858..>3936)
                     /locus_tag="VNU01_02375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWH31499.1"
                     /translation="ARLEELSRSRDQLRQSLSRLGQTLSSSLDLNRTLAVVVETAMDT
                     FEAERGALLLFTPERDALYVKVGRNLPDGMAPLRVDEGVAGWVARTGTAVRLPADAAA
                     TPAPAQGELVGVQQITVPLLGRGRTLGVLSLTRDAPELPFSADDLDAIQTFAGQASGA
                     IENVMLHHEARRLSVTDALTGLWNFRYLQLQAERELESSERFDRPMSLLICDLDHFKS
                     VNDEFGHQVGDDVLVEVAARLRDATRVPDVVARYGGEEFVVLLPGTDPEGAAATAERI
                     RAAVADVPVTTSAIRRDGHPLELQVTCSVGVATHPRDGRTVAALLRSADAAMYNAKRG
                     GRNRVVVAAGGMAPSAKPRKRKDG"
BASE COUNT          474 a         1570 c         1379 g          513 t
ORIGIN      
        1 gccgtgaccg tcgacgagcg gctcgagcgc cgcgggcgcg ctcaccggcg ccagtcctgc
       61 ttgccgccgg tcttctcgag cagctcgatc cgctcgatcg tcacgccgcg ctcgacgccc
      121 ttgaccatgt cgtagacgct cagcgcggcc accgccgccg cggtcagcgc ctccatctcc
      181 acgccggtgc ggtccgccgc ccggcaggtc accagcacgt cggcccgccc gtcgtcgggg
      241 tcgaccgtca cggacacgtc gacgccggcc agcgcgatcg ggtggcacag cgggatcagc
      301 tccggcgtgc gcttggccgc catgatcccc gcgacgcgcg ccaccgccag cgcgtcgccc
      361 ttcggcaggt gcccggacgc cagcagcgcc gccgtgccct ggctcatgcg caccacggcg
      421 cgcgccgtcg cggtgcgctg cgtgacctgc ttgccggcca cgtcgaccat gcgcgcctcg
      481 ccgtgctcgt ccaggtgcgt cagcccgggc aggccgtccg gctccgccgt gccgctcatg
      541 gccgctccag caggtggacc accagccgct cgccggcggc gacctgcgtg cgctcgggcg
      601 gcacctcggc gaggccgtcg gcgtccacga cgctgcgcag gatcccgctg ccctgctccc
      661 ccgtggggcg cgcgtgccac gcgccgccct cgcgccgcag gcgcacgcgc acgaactcga
      721 ccttctcgcg cggactgcgc agcggctcgt cgagcaccgc gctgatgcgc ggccggtagg
      781 tgtccgtgcg gccctgcagc cggcggatcg ccggccgcac gaacatctcg aagctcacga
      841 acgcgctcac cgggttgccg ggcagcccga acaccggcac cgcgcggtcc ggctcgcgcg
      901 gcagcagccc gaacgcctgc ggcatccccg ggcgcatccc cacccgcacg aagcgcacgt
      961 cgcccagccg cgccagcacc accttcacca ggtcgtagcg cccggcgctg acgccgccgg
     1021 acgtcaccac caggtcggcg tgcaccagcg cgccctcgaa cgcctcggtg agcgcgcgct
     1081 cgtcgtcgcg cacgatctgg tggcggtacg ccgacgcgcc cgcgtcgcgc gccatcgccg
     1141 tcagcgtgta ggagttcgcg tcgcggatct ggccgaggcc ggtcggctcc tccggctcca
     1201 ccagctcgtc gcccgtggag atcaccacca cgcgcggctg cggccgcacc agcacgcggg
     1261 ggtagcccag ggcggcgagc atcccgatgt cggccggcgc cagcacccga cccgacgaca
     1321 gcaccacctc gccgtggcgc acgtcctccc cggccatgcg cacgtgctgg cccggctcgg
     1381 cgccggcggc caggcgcacc acgccgggcc actcctcggc ctgctcgacc ggcaccaccg
     1441 cgtccgcgcc cggcggcatc ggcgcgcccg tcatgatgcg caccgcctgg ccgcggccca
     1501 ccggcgtgcg cgccggcgag cccgccgcgg actcgccgac cagtgccagc tccgcgcccg
     1561 gcaccgcgtc catcgccagc agcgcgtagc cgtccatcgc gctgttgggg aacggcggca
     1621 ggtcgctgac cgccacggcc tcctgcgcca gcacgcagcc ctgcgcgtcc aggatcccca
     1681 gctcgatcgg ctccagcggg cggatgcgcg ccagcacgtc cttgcggtgc tcggcgaacg
     1741 gcaccagcgg ctgtggctcg cgcggaccgt gcggcggcgc cgcgggcggg accgcggcgg
     1801 gcccggcctc cggcgggacc gcggccgggc gcgcctccgg tccgaacagc ggcggcggct
     1861 ccgggtccgt gtcgtcctgc ctgcggcgga agatcactcc agccccttca cgaactcctt
     1921 gaggaacgca cggaaggtgg gccccaggtc ggcgcggtgc gccgccagct ccaccatcgc
     1981 gcgaaggtag tcctgcttgg cacccacgtc gtagcgcagg ccttcgaact tcagcgcgcg
     2041 gatcggctcc tcctggcact gcgcgcgcat cgcgtcggtg agctggatct cgccgccgcg
     2101 cccgggccgc gtgccggcga tgtggtcgaa gatgtccggc gtcagcacat agcggccgat
     2161 gatgcacagg ttgctcggcg cgttggcctt gccgggcttc tccaccaggt cgcgcaccag
     2221 gtaggccccc gggttgtcgg ggtccggctc ggcgtcgatc acgccgtact ggctcagcgc
     2281 ctcgtcgccg aactccatga ccgccaccac gctgcggccg gtgcgctcgg acaggtcgac
     2341 catctgcgac agcagccgct cctgctcgcc gatcaggtcg tcgccgagca gcaccgcgaa
     2401 cggctcgtgg ccgacgtgat ggcgggcgca gccgaccgcg tggccgaggc ccagcggctg
     2461 gccctgccgg atgtagtgca ccagggcgag ctggctgacc gcgcgcaccg cggcgagcag
     2521 gtcctctttg ccggcggctg ccagctgccg ctccagctcc acggcgtggt cgaagtggtc
     2581 ctccagcgcg cgcttgcccc gcccggtgac gagcaggatg tcgtccaggc ccgccttcac
     2641 cgcctcctcc accacgtact ggatcgcggg cttgtccacg acgggcagca tttccttcgg
     2701 ctgcgccttg gtcgcgggca ggaagcgcgt gccgagtccg gcggccggga tgaccgcttt
     2761 gcgagcgcgc aaaaggaggt cctttcgtgt cgttccgcgg atgcagccgc atcgtagccg
     2821 gagccgtcgg ccggtggccg ggagtcgcgc ggcggggcta gccgtccttg cgcttgcgcg
     2881 gcttggccga cggcgccatc ccgccggccg ccaccacgac gcggttgcga ccgccgcgct
     2941 tggcgttgta catggccgcg tcggcgctgc gcagcagcgc ggccaccgtg cgcccgtcgc
     3001 gggggtgcgt ggccacgccg acgctgcagg tgacctgcag ctccagcggg tggccgtcgc
     3061 ggcggatcgc ggacgtggtc accggcacgt cggccaccgc cgcacggatg cgctcggcgg
     3121 tggcggccgc gccctccggg tccgtgccgg gcaacagcac cacgaactcc tcaccgccgt
     3181 agcgggccac cacgtccggc acgcgcgtcg cgtcgcgcag ccgcgcggcc acctccacca
     3241 gcacgtcgtc gccgacctgg tggccgaact cgtcgttgac cgacttgaag tggtccaggt
     3301 cgcagatcag cagcgacatc gggcggtcga agcgctccga gctctccagc tcgcgctccg
     3361 cctgcaactg caggtagcgg aagttccaca agcccgtgag cgcgtcggtc accgacaggc
     3421 gccgcgcctc gtggtgcagc atcacgttct cgatggcgcc cgacgcctgc cccgcgaacg
     3481 tctggatggc gtccaggtcg tcggcgctga acggcagctc gggcgcgtcg cgcgtcagcg
     3541 acagcacgcc cagcgtgcgg ccgcggccaa gcagcggcac ggtgatctgc tgcacgccga
     3601 ccagctcccc ttgtgcgggc gcgggtgtcg cggccgcgtc ggccggcagg cgcacggccg
     3661 tgcccgtgcg cgccacccag ccggccacgc cctcgtccac gcgcagcggc gccatcccgt
     3721 cgggcaggtt gcggcccacc ttcacgtaca gcgcgtcgcg ttccggcgtg aacagcagca
     3781 gggcgccgcg ctccgcctcg aacgtgtcca tggcggtctc gaccaccacc gccagcgtcc
     3841 ggttcaggtc caggctggac gacagcgtct ggccgaggcg cgacagcgac tggcgcagct
     3901 ggtcgcgcga gcggctgagc tcctccaggc gcgccg
//
LOCUS       DATMKC010000083         4085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_86763, whole genome shotgun sequence.
ACCESSION   DATMKC010000083 DATMKC010000000
VERSION     DATMKC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86763"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VNU01_02380"
     CDS             <1..1062
                     /locus_tag="VNU01_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWH31500.1"
                     /translation="LGDVPTQLLLSAKGHVDNLVREFALAAGGAASGTTAAVPAHLAQ
                     LIDTVVHRFAEARDSIKRQALAAARQGHDHLRLELRLPVSAARAGEEYLAALDQADAY
                     CRAARLLTLESPPQHRVFRRWYVGELVAQLRQAAEGHEPAPAETFEERLLREIDAFAA
                     AERTAERFARLHSLSAALASAGTPEAVTEAVLREGVAALGATGGAVLVASPAGELAVP
                     TAVGYGEQVMARLRAESPDAELPAAVALRTGEPVWLESREARDARFPELEGVEPGTLS
                     MCAVPLIVDGRTVGAMRFSFSRNRLFDAEERDFIDTMAAQTAQALDRALMYRQWQEPG
                     RRPPGAPGGGESGMGIEPT"
     gene            complement(1034..1105)
                     /locus_tag="VNU01_02385"
     tRNA            complement(1034..1105)
                     /locus_tag="VNU01_02385"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1070..1072),aa:Cys,seq:gca)
     gene            complement(1123..1353)
                     /locus_tag="VNU01_02390"
     CDS             complement(1123..1353)
                     /locus_tag="VNU01_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31501.1"
                     /translation="MRAPRLGPDQRIAAHVTRVGRFRRIDPSKGRRGPSRWALLGRLG
                     WAVLVAESLRRAWRRSATGDSAAGDSAASGPG"
     gene            1597..1788
                     /locus_tag="VNU01_02395"
     CDS             1597..1788
                     /locus_tag="VNU01_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31502.1"
                     /translation="MERELLHRLLSQAFLYDDPNAYRAGVQAAWGALETLRSAPPQRS
                     DDPAPTQPRVAEAPVRATG"
     gene            complement(1785..2435)
                     /locus_tag="VNU01_02400"
     CDS             complement(1785..2435)
                     /locus_tag="VNU01_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HWH31503.1"
                     /translation="MNTLAWTALAVAALAAVLDWYAVARERTRLEYVAKPATLVLLIG
                     VALALEPADATQRAWWVGALVFSLAGDVFLMLPGRFVPGLASFLVAHLAYIAGFWLGP
                     VGGTGPLEFLVAVLPVAVLAVVLGPRILAGARAHDGRLVGPVANYLVVICAMAASALL
                     TGDPVAIAGAGLFFGSDAMIGFRNFVASKPWMDLAIITTYHVGQALLVLSLLPGPA"
     gene            2525..3163
                     /locus_tag="VNU01_02405"
     CDS             2525..3163
                     /locus_tag="VNU01_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31504.1"
                     /translation="MRRLMAVCLLLGSLLAPGAARANHEIPDESHLPNAEPYARAALS
                     LAQNIRPIPWGTTLHIDQSEETWRIDCTDGQGNTWPGECPFVLEGALDLDPHYGPLDD
                     PGCSIPRGHSGEGWFDPEWTLGLPEVGLTSEPYAGATIDDARFARTPYVDQARAGALL
                     IEGRWSDEHGRSDRFLAEFKMTFPAWPPGSTCSSSVRHYFIDGHVMLLGVLR"
     gene            3221..>4085
                     /locus_tag="VNU01_02410"
     CDS             3221..>4085
                     /locus_tag="VNU01_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWH31505.1"
                     /translation="MAGMGDRAAIIGAGPAGLAAGAALKAAGVPFTIYERHGDVGGLW
                     DIDNPGTPVYETAHFISSRTLSGFDGFPFPDDYPDYPGRRQVLDYLRAFAERHDLRPH
                     VATGHELARAEPLADGGWSLEFTGGRADLARWLVAANGHLWEPNLPAYPGEFHGEAYH
                     ARDYRSPEQLWGRRVLVVGGGNSGVDIACDAATAASGAAISLRRGYWFVPKHVFGVPA
                     DVIGHGGPTLPPWLERRVIGLLLRLTVGDLRRYGLPKPDHQPLSSHPILNSQLLHFLA
                     HGDVAARPDVAE"
BASE COUNT          573 a         1498 c         1503 g          511 t
ORIGIN      
        1 ctcggggacg tgccgacgca gctgctgctg tcggccaagg gccacgtcga caacctggtc
       61 cgggagttcg cgctcgcggc gggcggcgcg gcgtccggga cgacggccgc ggtgccggcg
      121 cacctggcgc agctcatcga caccgtggtg caccggttcg ccgaggcgcg cgactccatc
      181 aagcgccagg cgctcgcggc ggcgcgccag ggccacgacc acctgcgcct ggagctccgg
      241 ctgccggtga gcgccgcacg ggcgggcgag gagtacctgg ccgcactcga ccaggccgac
      301 gcctactgcc gcgccgcacg gctgctcacg ctggagtcac cgccgcagca tcgggtgttc
      361 cgccgctggt acgtcggcga gctggtcgcg caactgcggc aggccgccga ggggcacgag
      421 ccggcgccgg ccgagacctt cgaggagcgc cttctgcgcg agatcgacgc gttcgccgcg
      481 gccgagcgca cggccgagcg tttcgcgcgg ctgcactcgc tgtccgccgc cctggcgtcg
      541 gcggggacgc cggaggcggt caccgaagcg gtcctgcggg agggtgtcgc cgcgctcggc
      601 gccaccggcg gggcggtgct ggtcgcgtcg cctgccggcg agctcgccgt gccgacggcc
      661 gtgggctacg gcgagcaggt gatggcgcgg ctgcgcgcgg agtcgcccga cgccgagctg
      721 ccggcggcgg tggcgctgcg caccggcgag ccggtgtggc tggagtcgcg ggaagcgcgg
      781 gacgcgcgct tcccggaact ggagggtgtg gagccgggca ccctgtcgat gtgcgcggtc
      841 ccgctgatcg tggacggccg caccgtcggc gccatgcgct tctcgttctc ccgcaaccgg
      901 ctgttcgacg ccgaggagcg cgacttcatc gacacgatgg ccgcgcagac cgcgcaagcc
      961 ctggaccgcg ccctgatgta ccggcaatgg caggaaccgg gacggcggcc gccgggggcg
     1021 cctggaggcg gagagtccgg gatgggaatc gaacccacgt agacggtttt gcagaccgtt
     1081 gcctgaacca ctcggctacc cggacgtagc aaaggcaggg tatcaaccag ggccggacgc
     1141 tgccgagtcc ccggccgccg agtcccccgt ggccgaccgc cgccaggcgc ggcgcaggga
     1201 ctccgccacc agcacggccc agcccagccg accgagcagc gcccagcgcg acggtccccg
     1261 ccggcccttg gacgggtcga tgcggcggaa gcggcccacg cgcgtgacgt gcgcggcgat
     1321 gcgctggtcc ggccccaagc gcggtgccct catcggcttc tccctgtggc ggtcggtcgg
     1381 cgtgctgccc gccgccgagc atatggcacc tctggcacgc gccaccgggc ggtcgccccc
     1441 gccgacccgg ggatctttgc gcaacacagt ggtttcgaga cgttcacggt gggtacacac
     1501 ggcggccacc tgcgttgtgc accctgtggt cgcagacgtt ggtcccggac gacaacaagc
     1561 aaccggtttt ccttgtggaa acggggggat gcagccatgg agcgggaact tctccaccgc
     1621 ctgttgagcc aggcgttcct ctatgacgac ccgaacgcct accgcgccgg ggtgcaggcc
     1681 gcctggggtg ccctggagac gctgcggagc gcgccgccgc agcgctcgga cgaccccgcg
     1741 ccgacgcagc cgcgcgtcgc cgaggcgccc gtgcgcgcga ccggctaagc cggcccgggc
     1801 agcagggaca gcaccagcaa cgcctggccg acgtgatagg tcgtgatgat ggccaggtcc
     1861 atccacggct tgctcgccac gaagttgcgg aagccgatca tcgcgtcgga gccgaagaac
     1921 agccccgcgc cggcgatggc caccgggtcg cccgtcagca gggcgctggc cgccatcgcg
     1981 cagatgacca ccaggtagtt ggccaccggc cccaccaggc gcccgtcgtg ggcacgggct
     2041 ccggccagga tgcgcgggcc cagcaccacg gccaggaccg cgaccggcag gaccgccacc
     2101 aggaactcca gcgggcccgt gccgccgacc gggccgagcc agaagcccgc gatgtaggcc
     2161 aggtgcgcga ccaggaagct cgccaggccc gggacgaagc gccccggcag catcaggaag
     2221 acgtcgccgg ccaggctgaa caccagcgcg ccgacccacc acgcgcgctg cgtggcgtcg
     2281 gccggctcca gcgccagcgc gacgccgatc agcagcacca gggtggcggg cttggcgacg
     2341 tactccaggc gcgtgcgttc ccgcgcgacc gcgtaccagt ccagcacggc ggcgagcgcc
     2401 gcgacggcga gcgcggtcca cgcgagcgtg ttcatggccg acagcgtaaa ggcggacgcg
     2461 aaccggcggc cgtaggatct ccccgtggcg gtcggcggga ccgcctcgga cacgggggat
     2521 tcctatgcga cgactgatgg cggtgtgcct gctgctcggc tccctgctcg cgcccggtgc
     2581 cgcgcgcgcg aaccatgaga ttcccgacga gagtcacctt ccgaacgctg agccctacgc
     2641 gcgagcagca ctgtccctgg cgcagaacat ccgtccgatc ccgtggggga ccacgctgca
     2701 catcgatcag tccgaggaga catggcgcat cgactgcacc gacggccagg gcaacacctg
     2761 gccaggcgag tgcccgttcg tgcttgaggg cgcgctggac ttggatccgc actacggccc
     2821 gctggacgac cccggctgct cgattcctcg aggccacagt ggcgaggggt ggttcgaccc
     2881 ggagtggacg ctgggcctgc cggaggtggg gctgacgagc gagccctacg ccggtgccac
     2941 catcgacgac gcccggttcg cacgaacacc gtacgtcgac caagcacgcg cgggggctct
     3001 gctgatcgaa ggccgatggt ccgacgagca cggccgcagc gaccgcttcc tcgcggagtt
     3061 caagatgacg ttcccggcgt ggcccccggg cagcacctgc tcgagctccg ttcggcacta
     3121 cttcatcgac gggcacgtca tgctgctggg ggtccttcgc tgacatcgcc gcccgcgccc
     3181 gccgccgcgt atcgtgaggg ggcgggcgcg aggaggcggc atggcgggca tgggtgatcg
     3241 ggcggccatc atcggggcgg ggccggcggg cctggcggcg ggagcggcgc tgaaggcggc
     3301 gggcgtgccg ttcacgatct acgagcgcca cggcgacgtg ggcgggctgt gggacatcga
     3361 caaccccggc acgccggtct acgagacggc gcacttcatc tcctcgcgca cgctgtcggg
     3421 cttcgacggc ttccccttcc cggacgacta cccggactat cccggccgcc ggcaggtgct
     3481 ggactacctg cgcgccttcg ccgagcgcca cgaccttcgc ccccacgtcg ccaccggcca
     3541 cgagctggcg cgcgccgagc cgctggccga cggcggttgg agcctggagt tcaccggcgg
     3601 acgcgcggac ctggcccgct ggctggtggc cgccaacggg cacctgtggg agcccaacct
     3661 gccggcctac ccgggcgagt tccacggcga ggcctaccac gcgcgcgact accgctcacc
     3721 ggaacagctg tggggccgcc gcgtgctggt ggtgggcggc ggcaactccg gcgtggacat
     3781 cgcctgcgac gccgcgaccg ccgccagcgg ggcggcgatc agcctgcggc gcggttactg
     3841 gttcgtgccc aagcacgtgt tcggcgtgcc cgcggacgtc atcggacacg gcgggccgac
     3901 cctgcccccc tggctggagc gccgcgtcat cgggctgctc ctgcggctca ccgtcgggga
     3961 cctgcgccgc tacggcctgc ccaagccgga ccaccagccg ctgtccagcc atcccatcct
     4021 gaacagccag ctgctgcact tcctggccca cggggacgtg gcggcgcggc ccgacgtggc
     4081 ggagc
//
LOCUS       DATMKC010000084         6194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_40815, whole genome shotgun sequence.
ACCESSION   DATMKC010000084 DATMKC010000000
VERSION     DATMKC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40815"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VNU01_02415"
     CDS             <1..1109
                     /locus_tag="VNU01_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31506.1"
                     /translation="EEQIVIALTKWLNKRGGIRSRTVVPVIHKTNPTSGSFSTQAQAA
                     CATFTEDNQVFAAGSAPAAGSEVLAACMASKRTPFLAHNYWPFGEQHFRQWAGYFYQP
                     GRAAGERHWAASVDALAGAGFFKGAKVGIVHFDAPHFRHLVKDVVVPGLARHGVKAPT
                     EAAVSTIYQASDVSKVSAEANSIILRFRAEGVTHVLFVEYQGTIPFFFLPQAESQGYR
                     PAYGLVSSNNPSTVAKQSPAAQLQKVTGVGWNPSGDTLKGNYHPNATAAQCEMAMREG
                     GYAVDYSAAGFYLYPHCDTLLFLKAAIERAQELTPQGLAAAVAGLGESYLSPQTMVTR
                     LGPGRADGSDGHRLFKFDAACACFAYTGPVRRLG"
     gene            1188..2822
                     /locus_tag="VNU01_02420"
     CDS             1188..2822
                     /locus_tag="VNU01_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH31507.1"
                     /translation="MTQDVTLDRTLVLPHLIDALAAEDATAVLVEDVEDVPGGRSYTR
                     AELQDAYRRWAGALRGAGVSAGDTVLTMLANRAETYPVWLGISWLRAIEAPMHTQYKG
                     PMLRYGANYSEARVGVVDGRFLPLFAELAGELEHLRLLVVVDPPAKLPDLGVDVILAE
                     RFLDGAARADDEPGPAYHDIACMVFTSGTTGPSKGVLMPWAELYEFPAFQKPADMLAP
                     GQGMYQVLPQFHAAGKVGLYVAALARARAVCREVFSITEFWPDVRRYDCHAAGVVGPL
                     AEILIQMPEHPDEIGNPLERVMMGPLIGEVDLFRKRFGVEVVTGYGMTEIGVPLASDG
                     YQLANNTSCGRVREGYELRIVNEYDEPVADGDVGELIVRHRYPWRLNAGYWRMPEATA
                     EAWRNGWFHTGDAFRRDDDGNYYFVDRIKDAMRRRGENISSFEVEMHIAEHPDVFEVA
                     AMPVRSPLGEDEVGVVVSAEESLTAEALIEWLIPRMPRFMIPRYVKLVDEELPRTATM
                     RIQKFKLKESFPDEHTFDREAAGIVLPKDTEQAVKG"
     gene            2819..3250
                     /locus_tag="VNU01_02425"
     CDS             2819..3250
                     /locus_tag="VNU01_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWH31508.1"
                     /translation="MTATTSRPIAEGLFTWPADEPRLIGAACAACGTTVFPRRSSCPK
                     CGAQDMAERLLSPTGTLWTWTTQGFLPKAPYGGPETDETFQPFALGYVELAGEVRVEA
                     RLTEPDPARLRIGMPMRLVVVPFRTDPDGTEVVSFAFAPAD"
     gene            3262..4422
                     /locus_tag="VNU01_02430"
     CDS             3262..4422
                     /locus_tag="VNU01_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWH31509.1"
                     /translation="MDIAIVGIGIHPFGRHEGVSGAQMGAHAVREALRDAGAAWPDVQ
                     FAAGGSLAYALPGGGAMPDTLVNELGLTGLPFVNVANGCATSATAVLSAANAILAGAA
                     DLAVAVGFDKHQRGHFNAEPETNGVDDWYGELGMMITTQFFAMKINRYLHEHGIGHDI
                     LAKVAAKAFRNGALNPNAWRRQALSEEEIVGARMLNYPLTQYMFCSPDEGAAALVLCR
                     ADQAHRYTDRPVYLRAVTLRTRKFGTFEVFSPWVALEEADTPVREASRACFEAAGLGP
                     EDIDLAQIQDSESGAEIMHMAENGLCKDGEQEALIREGATEIGGRLPVNTDGGLLANG
                     EPIGASGMRQFYETVLQLRGDAGPRQVPGNPRVGYTQVYGAPGVGACALLTT"
     gene            4419..5447
                     /locus_tag="VNU01_02435"
     CDS             4419..5447
                     /locus_tag="VNU01_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWH31510.1"
                     /translation="MSATVPARAAGGGPAAAERVAGGVWQIRVPVPFGPTGGTLVYLL
                     EADGGPVLVDAGWDTPAGWEALTAGITATGHDVADVRGVVVTHFHPDHHGMSGRVREA
                     AGAWVAMHPVEAEMVTRQRDGGVADFTGIAAAFFAAGAPAASYDDVPDALPVALPPAA
                     VPDRLLADGQLADLPGRRLRALWTPGHSPGHLSFHLEDARLLMTGDHILPRISPHVGL
                     WMQADDADPLGDFLASLERLSTLDVDEVLPAHEHRFPGLRERIAELRMHHAARLAAVQ
                     ARLADSPATLWEVAEAMPWEARWQDVPAVMRHIALGEAASHVRHLERLGRVVRVDSGG
                     PTRFTVPA"
BASE COUNT          850 a         2251 c         2256 g          837 t
ORIGIN      
        1 aggaggagca gatcgtcatc gcgctgacga agtggctgaa caagcggggc ggcatccgca
       61 gccgcacggt ggtgccggtg atccacaaga ccaacccgac gtcgggcagc ttctccaccc
      121 aggcccaggc cgcctgcgcg accttcaccg aggacaacca ggtgttcgcc gcgggctccg
      181 cgccggcggc gggcagcgag gtgctcgccg cctgcatggc gtccaagcgc accccgttcc
      241 tcgcgcacaa ctactggccg ttcggggagc agcacttccg ccagtgggcg ggctacttct
      301 accagcccgg ccgggccgcc ggggagcgcc actgggccgc ctccgtcgac gccctggccg
      361 gggcgggctt cttcaagggc gccaaggtgg gcatcgtgca cttcgacgcc ccgcacttcc
      421 gccacctcgt gaaggacgtc gtcgtgccgg gcctggcgcg ccacggcgtg aaggcgccca
      481 cggaggccgc cgtgtcgacc atctaccagg cctccgacgt gagcaaggtc agcgccgagg
      541 ccaacagcat catcctgcgg ttccgggcgg agggcgtcac gcacgtgctg ttcgtggagt
      601 accagggcac catcccgttc ttcttcctgc cgcaggcgga gagccagggc taccgcccgg
      661 cctacgggct ggtgtcgtcc aacaacccct ccaccgtggc caagcagtcc cccgccgcgc
      721 agctgcagaa ggtgaccgga gtggggtgga acccgtcggg cgacacgctg aagggcaact
      781 accaccccaa cgccacggcc gcccagtgcg agatggcgat gcgcgagggc ggctacgccg
      841 tggactactc ggcggcgggc ttctacctgt acccgcactg cgacacgctg ctgttcctga
      901 aggccgcgat cgagcgcgcc caggagctca cgccccaggg cctggccgct gcggtcgccg
      961 ggctgggcga gtcctacctg tcgccgcaga ccatggtcac ccgcctgggt cccgggcgcg
     1021 cggacggcag cgacggccac cggctgttca agttcgatgc ggcctgcgcg tgcttcgcct
     1081 acaccggacc ggttcggcgc ctggggtgag cgcggccctg gacgccgagc cgacgctgcg
     1141 ctaatttaaa ataacattgg accccggccc gctggaggcg ccccctcatg acacaggacg
     1201 tcacgctcga ccgcacgctg gtcctgcccc acctgatcga cgcgctggcg gcggaggacg
     1261 cgaccgcggt cctggtggag gacgtggagg acgtgccggg cggccgctcc tacacgcgcg
     1321 cggagctgca ggacgcctac cggcgctggg ccggcgcgct gcgcggggcc ggggtgagcg
     1381 cgggcgacac cgtgctgacg atgctggcca accgcgcgga gacctacccg gtgtggctgg
     1441 gcatctcgtg gctgcgcgcc atcgaggcgc cgatgcacac ccagtacaag gggccgatgc
     1501 tgcgctacgg cgcgaactac agcgaggcgc gcgtcggcgt ggtggacggc cgcttcctgc
     1561 cgctgttcgc cgagctcgcc ggggagctgg agcacctgcg gctgctggtg gtggtcgacc
     1621 cgccggcgaa gctgccggac ctgggcgtcg acgtgatcct ggccgaacgg ttcctcgacg
     1681 gcgcggcgcg cgcggacgac gagccggggc ctgcgtatca cgacatcgcc tgcatggtgt
     1741 tcacgtcggg caccaccggc ccgtccaagg gcgtgctgat gccgtgggcc gagctgtacg
     1801 agttccccgc gttccagaag ccggcggaca tgctggcgcc cggccagggc atgtaccagg
     1861 tgctgccgca gttccacgcc gccggcaagg tcggcttgta cgtggcggcc ctggcgcgcg
     1921 cccgcgcggt gtgccgggag gtgttctcca tcaccgagtt ctggccggac gtgcgccgct
     1981 acgactgcca cgcggcgggc gtggtcggcc cgctggcgga gatcctcatc cagatgccgg
     2041 agcacccgga cgagatcggc aacccgctgg agcgagtgat gatggggccg ttgatcggcg
     2101 aggtggacct gttccgcaag cgcttcggcg tggaggtggt gaccggctac ggcatgaccg
     2161 agatcggcgt cccgctggcc agcgacggct accagctcgc caacaacacc agctgcggcc
     2221 gcgtgcgcga gggctacgag ctgcgcatcg tcaacgagta cgacgagccg gtggcggacg
     2281 gcgacgtggg cgagctgatc gtgcgccacc gctacccgtg gcggctcaac gccggctact
     2341 ggcgcatgcc ggaggcgacc gcggaggcgt ggcgcaacgg ctggttccac accggcgacg
     2401 cgttccggcg ggacgacgac ggcaactact acttcgtcga ccgcatcaag gacgcgatgc
     2461 gccggcgcgg ggagaacatc tcctcgttcg aggtggagat gcacatcgcc gagcaccctg
     2521 acgtgttcga ggtcgcggcg atgccggtgc gctccccgct gggcgaggac gaggtcggcg
     2581 tggtcgtctc ggccgaggag tccctgacgg ccgaagcgct gatcgaatgg ctcatcccgc
     2641 gcatgccgcg cttcatgatc ccccgctacg tgaagctggt ggacgaggag ctgccgcgca
     2701 cggccacgat gcgcatccag aagttcaagc tcaaggagtc cttcccggac gagcacacgt
     2761 tcgatcgcga ggcggccggc atcgtgctgc ccaaggacac cgagcaggcg gtgaagggat
     2821 gacggcgacc accagccggc cgatcgcgga gggcctgttc acctggccgg ccgacgaacc
     2881 gcgcctgatc ggcgccgcgt gcgccgcctg cggcaccacc gtgttcccgc gccggtcctc
     2941 ctgtcccaag tgcggcgcgc aggacatggc cgagcgcctg ctgtcgccga ccgggacgct
     3001 gtggacctgg acgacgcagg gcttcctgcc caaggccccg tacggcgggc cggagaccga
     3061 cgagacgttc cagccgttcg ccctgggcta cgtggagctg gccggcgagg tgcgcgtgga
     3121 ggcgcgcctg accgagcccg accctgcgcg cctgcgcatc ggcatgccga tgcgcctggt
     3181 ggtggtcccg ttccgcaccg acccggacgg caccgaggtc gtgtccttcg ccttcgcccc
     3241 cgccgactga caggagcatc catggacatc gcgatcgtcg gcatcggcat ccacccgttc
     3301 gggcggcacg agggcgtgtc gggcgcgcag atgggcgccc acgccgtgcg cgaggcactg
     3361 cgcgacgccg gcgccgcatg gccggacgtg cagttcgcgg cgggcggcag cctggcctac
     3421 gcgctgccgg gcggcggcgc gatgccggac accctggtga acgagctggg cctgaccggg
     3481 ctgccgttcg tgaacgtggc gaacggctgc gcgacctcgg ccaccgcggt gctgtccgcc
     3541 gcgaacgcca tcttggccgg cgccgcggac ctggccgtcg cggtcggctt cgacaagcac
     3601 cagcgcggcc acttcaacgc cgagccggag accaacggcg tggacgactg gtacggcgag
     3661 ctgggcatga tgatcaccac ccagttcttc gccatgaaga tcaaccgcta cctgcacgag
     3721 cacggcatcg ggcacgacat cctggccaag gtcgccgcga aggcgttccg caacggcgcg
     3781 ctcaacccca acgcctggcg ccgccaggcg ttgagcgagg aggagatcgt gggcgcgcgg
     3841 atgctcaact acccgctcac gcagtacatg ttctgctcgc ccgacgaggg cgccgccgcg
     3901 ctggtgctgt gccgcgccga ccaggcgcac cgctacaccg accggcccgt gtacctgcgc
     3961 gccgtgacgc tgcgcacacg caagttcggc accttcgagg tgttcagccc gtgggtggcg
     4021 ctggaggagg cggacacccc ggtgcgggag gcctcgcgcg cctgcttcga ggcggcgggc
     4081 ctgggaccgg aggacatcga cctggcgcag atccaggaca gcgagtccgg ggcggagatc
     4141 atgcacatgg ccgagaacgg cctgtgcaag gacggcgagc aggaggcgct catccgcgag
     4201 ggcgcgaccg agatcggcgg ccggctgccg gtcaacaccg acggcggcct gctggccaac
     4261 ggcgagccga tcggcgcctc cggcatgcgg cagttctacg agacggtcct gcagctgcgc
     4321 ggcgacgccg gtccccggca ggtgcccggc aacccgcgcg tgggctacac gcaggtctac
     4381 ggcgcgcccg gtgtcggcgc gtgcgcgctg ctgacgacgt gagcgccacg gtcccggcgc
     4441 gggcggccgg cggcgggccg gccgccgccg agcgggtggc gggcggcgtc tggcagatcc
     4501 gcgtgcccgt gccgttcggc cccaccggcg gcacgctggt ctacctgctg gaggcggacg
     4561 gcggccccgt gctggtcgac gccggctggg acaccccggc cggatgggag gccctcaccg
     4621 ccggcatcac cgccacggga cacgacgtcg ccgacgtgcg cggcgtggtg gtcacgcact
     4681 tccatccgga ccaccacggc atgtccggcc gcgtgcgcga ggccgccggc gcctgggtcg
     4741 cgatgcaccc ggtcgaagcc gagatggtca cgcgccagcg cgacggcggc gtcgcggact
     4801 tcaccggcat cgcggcggcg ttcttcgccg cgggtgcccc ggccgcctcc tacgacgacg
     4861 tgcccgacgc cctgccggtg gcgctgccgc ccgccgccgt gcccgaccgg ttgctggcgg
     4921 acggacagct cgccgacctc cccgggcgcc gcctgcgggc gctgtggacg ccgggccact
     4981 cccccggcca cctgagcttc cacctggagg acgcccggct gctcatgacg ggggaccaca
     5041 tcctcccgcg catcagcccc cacgtcggcc tgtggatgca agcggacgac gccgacccac
     5101 tgggggactt cctcgcctcg ctggagcggc tgtccacgct cgacgtcgac gaggtcctgc
     5161 ccgcccacga gcaccgcttc cccggcctgc gcgagcgcat cgccgagctg cggatgcacc
     5221 acgccgcgcg cctcgccgcc gtccaggcgc gcctcgcgga cagccccgcg accctgtggg
     5281 aggtcgccga ggcgatgccg tgggaggcgc gctggcagga cgtgccggcg gtcatgcgcc
     5341 acatcgccct cggcgaagcg gcctcgcacg tccgccacct cgagcgcctc ggccgtgtcg
     5401 tccgggtgga cagcggcggc cccacccgct tcacggtgcc ggcctaaccg gtctgtgccc
     5461 gcgcgcggcg gcgctcgccc gcgtgtgtgc ggtcgcgctc gcccgcgcgt gtgcggtcgc
     5521 gctcgcccgc gtgtccgcgg tcggcgtggg aaccggaccc ctcctcgctc ctcgcccggc
     5581 tgatatcgcg gtgtcgggcg tcggcggcca ggttgcggcc gcgcgtgcgt gatgccgcga
     5641 gccgggctcg tcctcgtcgg tctccggttc ccacgccgac cgccaccccg cccgcctgtt
     5701 ggcggtgtgt gggcgggctt cgcgccccac ctggcgggtg gggtggcgga tcagccggta
     5761 tgcgcgagcg cgagggctgg gttctctatg ttgttgtcaa ggtgcagtgg ttccggtggt
     5821 gcgggtgctt ccggtggtgc gtggccgggt gccgggggtg gcccggcggt gtagcggccg
     5881 tgttcttgcc ggacttgcca gccgtgttgg tggatgtggc tgtggcagcg ccagcagacc
     5941 aggacgaggt tgtccaggtc tgctggcgct gccacagcca catccaccag cacggctggc
     6001 acgtccggca agaacacggc cgctacaccg ccgggccacc cccggccgac ggccccgcac
     6061 caccggaacc actgcacctt gacaacaaca tagagaaacc agcgctcgcc cataccggct
     6121 gacccgccac cccacccgcc aggtggggcg cgaagcccgc ccacacaccg ccaacaggcg
     6181 ggcggggtgg cggt
//
LOCUS       DATMKC010000085         5020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_205857, whole genome shotgun sequence.
ACCESSION   DATMKC010000085 DATMKC010000000
VERSION     DATMKC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205857"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VNU01_02440"
     CDS             complement(<1..387)
                     /locus_tag="VNU01_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWH31511.1"
                     /translation="MRALPERFLPNHSHPSPRRYGLEIQGAGAWTVATGDGRCAIWRG
                     ADDRVHAWLRTDAESWLAIVRGDLSGLDLFLAGELQVTGDLNEALRLETMFAAPPGTP
                     GEVRGGRLVRYRLDPRRSGRVVALGDG"
     gene            453..2228
                     /locus_tag="VNU01_02445"
     CDS             453..2228
                     /locus_tag="VNU01_02445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWH31512.1"
                     /translation="MPAPNASAEPPTSDIGALLSGLNAEQRAAAEAVRGAVCILAGAG
                     TGKTRTITHRIAAQIVSQTARPDQVLAVTFTEKAAGELRARLAALEVPGPVRAATFHA
                     AAWAQLRFFWRRVSDQPLPEVLPSKLPLLGRAALKLRAEARDLASEIEWAKARGVEPE
                     GYWAAAVGRDLPVPADVMADVYAGYERAKAEQHAIDYEDMILRTAVLLESDAEAAEVV
                     RDRYRFLTVDEFQDVNPAQWRLLRAWLGDSDELCVVGDDDQSIYGFTGATPEYLLTFT
                     KRFPTARQFRLSRNYRSTPQILDLANGVLGRRSAGRRLVAAGLPGPAPRLEEFATGED
                     EVRGVTDAVAGLVGGGVRPGEIAICFRVNRQSEPFESALRDAGIPYVVRGEGFFAQPE
                     VRQAVRALRVAAEQAAAPPARGAVPPVGGAAPARPPRLDREVERVLREALAFHPKREP
                     SGRAAKERWRNLTTLLELARARAQAGAATGLAAWVAELEERAAAGHDTADDNGAVTLV
                     TLHKAKGLEFDAVFLVGVEEGLLPIGHAKDDGSIAEERRLLYVGVTRARRHLWISWAR
                     SRPGSGGGPSRRRPSRFLHWLRRRA"
     gene            2240..3115
                     /locus_tag="VNU01_02450"
     CDS             2240..3115
                     /locus_tag="VNU01_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH31513.1"
                     /translation="MHANAEAHAAAGRHVETPAGRVFVREDGAANGGTPVVLLHGVPV
                     SSYVWRPVLPLLAGAGLHAVAPDFPGLGLSDRPDPDDFDYSFQGLHVALEQTLDALGI
                     ARAHLVVHDIGGPIGFLLAARAPERIASLTVLNTWVDLDTHRKPWPMWLFTKPGVDKA
                     TVAAMTAPTAWALLQQVGVKRLRAIGLGDAAAIVDLLRHADGGRAFLAIMRGFEQSPA
                     VRRELEEALARRAYPAAVVWGRHDPAIGAARAAHLAGLLHCGEPAFLDAKHFLMLDQP
                     AAVADAIARTAHAAA"
     gene            3112..3798
                     /locus_tag="VNU01_02455"
     CDS             3112..3798
                     /locus_tag="VNU01_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWH31514.1"
                     /translation="MSPLPDRPGDVAVAVDVVVLSLAGERAEPVFLAQPRRRDPYHGD
                     WSLPGGIVREREDLEAAVRRALARATALERVRHLEQLGTFGDPARDPRGRVVSVSYLA
                     LLPEPGPAGAGAGWLPATAPPPLAFDHAEILAAALERLRGKLSYSTVAYGLVGDEFTL
                     SELQAVYEAVLGEELDKRNFRKKVLALGVLEEAEGMRRGAHRPARLYRFRHRELVLFD
                     DVVTRAGTAL"
     gene            3795..>5020
                     /gene="pncB"
                     /locus_tag="VNU01_02460"
     CDS             3795..>5020
                     /gene="pncB"
                     /locus_tag="VNU01_02460"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019357 - nicotinate nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWH31515.1"
                     /translation="MSGYGATSALLTDLYELTMMAGYFDAGRHREQATFDLFFRRPPD
                     GVDVVVACGLDPALEYLAQLRFTDAELAYLRGLELFSPAFLDCLRELRFTGEVWAMPE
                     GTPVFPDEPLLRVSGPLLEAQLVETALLNRVCYATLVASVAAQVVDAAGGKDVLEFGT
                     RRAHGPDGALTGSRAAVIGGCGATSNTEAGMRFGVPVSGTQAHAWIMSFPSELDAFRC
                     YAETFPDSTVLLVDTYDTLGIGVPNAITVARELAERGHRLRGIRLDSGDLLELSRAAR
                     ALLDEAGLADVRILASGDLDAERVRALEAAGAPIDGYGVGTALLTAKRDPALSGVYKL
                     AEVGGEAVLKISSDEAKTTSPGRKQVWRDGGGDVIGLEGEDHPGRPLLTNVMHGGQRL
                     RHPEPVMRLREGSMAEV"
BASE COUNT          602 a         1876 c         1910 g          632 t
ORIGIN      
        1 gccgtcgccc agcgccacca cgcggccgct gcggcgcggg tccaggcggt agcgcaccag
       61 gcggccgccg cgcacctccc ccggcgtgcc gggcggcgcg gcgaacatcg tctccagccg
      121 cagcgcctcg ttgaggtcgc cggtcacctg cagctcgccg gccaggaaca ggtccaggcc
      181 ggacaggtcg ccgcgcacga tcgccagcca cgactccgcg tcggtgcgca accaggcgtg
      241 cacccggtcg tcggcgccgc gccagatcgc gcagcggccg tccccggtcg ccacggtcca
      301 cgcgcccgcg ccctggatct ccaagccgta gcgccgcggg gatggatggc tgtggttggg
      361 caggaagcgc tcgggaagcg cgcgcacgct gtcggcgacc gcctcggaaa gagccgaggg
      421 ggccgtcgca gccctccgat acgctgcgcc caatgcctgc tccgaacgcg tctgccgaac
      481 cccccacctc agacatcggc gccctgctgt ccggcctgaa cgccgagcag cgcgccgccg
      541 ccgaggcggt gcgtggagca gtctgcatcc ttgcgggcgc ggggacgggc aagacgcgca
      601 cgatcacgca ccgcatcgcc gcgcagatcg tctcgcagac ggcccgtccc gaccaggtcc
      661 tcgccgtgac gttcacggag aaggccgccg gcgagctgcg cgcccgcctg gccgccctgg
      721 aggtccccgg gccggtccgc gccgccacgt tccatgccgc cgcgtgggcg cagctgcgct
      781 tcttctggcg ccgtgtgtcc gaccagccgc tgccggaggt gctgccgtcc aagctgccgc
      841 tgctgggccg cgcggcgctg aagctgcgcg ccgaggcgcg cgacctggcc agcgagatcg
      901 agtgggccaa ggcgcgcggc gtggagccgg agggctactg ggccgccgcc gtgggccggg
      961 acctgcccgt gcccgccgac gtcatggccg acgtgtacgc cggctacgag cgggccaaag
     1021 ccgagcagca cgccatcgac tacgaggaca tgatcctgcg caccgccgtg ctgctggagt
     1081 ccgacgccga ggccgccgag gtggtgcgcg accgctaccg cttcctcacc gtggacgagt
     1141 tccaggacgt caatccggcg cagtggcggc tgctgcgcgc ctggctgggc gactccgacg
     1201 agctgtgcgt ggtcggcgac gacgaccagt ccatctacgg cttcacgggc gccacgcccg
     1261 agtacctgct cacgttcacg aagcgcttcc ccaccgcgcg ccagttccgg ctgtcgcgca
     1321 actaccgctc caccccgcag atcctggacc tggccaacgg cgtgctgggg cggcgctccg
     1381 ccggccggcg cctggtggcg gccgggctgc ccggaccggc gccgcgcctg gaggagttcg
     1441 ccacgggcga ggacgaggtg cgcggcgtga ccgacgccgt ggcgggcctc gtcggcggcg
     1501 gcgtgcgccc gggcgagatc gccatttgct tccgcgtgaa ccgccagtcc gagccgttcg
     1561 agtcggcgct gcgcgacgcc ggcatcccct acgtcgtgcg cggcgagggc ttcttcgccc
     1621 agccggaggt gcgccaggcg gtgcgggcgc ttcgggtcgc ggcggagcag gccgccgcgc
     1681 cgccggcgcg cggcgcggtg cccccggtgg gcggcgcggc gccggcgcgc ccgccgcgcc
     1741 tggaccgcga ggtggagcgg gtgctgcgcg aggcgctggc gttccacccc aaacgcgagc
     1801 cgtcggggcg ggccgccaag gagcgctggc gcaacctcac cacgctgctg gagctggcgc
     1861 gggcacgcgc ccaggccggc gcggcgaccg gcctggcggc ctgggtggcc gagctggagg
     1921 agcgcgcagc cgcgggacac gacaccgccg acgacaacgg cgcggtcacg ctggtcaccc
     1981 tgcacaaggc caaggggctg gagttcgacg cggtgttcct ggtcggcgtg gaggagggcc
     2041 tgctgcccat cggccacgcc aaggacgacg gctcgatcgc cgaggagcgc cgcctgctgt
     2101 acgtgggcgt cacgcgggcc aggcggcacc tgtggatctc ctgggcacgc agccgtcccg
     2161 gctcgggcgg cgggccgtcg cggcggcgcc cctcacgttt ccttcactgg ctgaggcgtc
     2221 gcgcataggc tttgcagcca tgcacgccaa cgccgaggcg catgccgccg cgggacgcca
     2281 cgtcgagacg ccggccggcc gggtctttgt ccgtgaggac ggcgcggcca acggcggcac
     2341 gccggtggtc ctgctgcacg gcgtgcccgt gtccagctac gtgtggcggc cggtgctgcc
     2401 gctgctggcg ggcgcgggcc tgcacgccgt ggccccggac ttccccggcc tcggcctgtc
     2461 cgaccggccg gacccggacg acttcgacta ctccttccag ggcctgcacg tcgcgctgga
     2521 gcagaccctg gacgccctgg gcatcgcgcg cgcgcacctg gtggtgcacg acatcggcgg
     2581 gccgatcggc ttcctgctgg ccgcgcgggc acccgaacgg atcgccagcc tcaccgtgct
     2641 gaacacctgg gtggacctgg acacgcaccg caagccgtgg ccgatgtggc tgttcaccaa
     2701 gccgggcgtc gacaaggcca cggtggcggc catgaccgcg ccgacggcgt gggcgctcct
     2761 gcagcaggtg ggcgtgaagc gcctgcgcgc catcggcctg ggcgacgccg cggcgatcgt
     2821 ggacctgctg cgccacgccg acggcgggcg cgccttcctg gcgatcatgc gcggcttcga
     2881 gcagtccccc gccgtgcgca gggagctgga ggaggcgctg gcgcggcggg cctacccggc
     2941 cgccgtggtg tggggccgtc acgacccggc catcggcgcc gcccgcgccg cacacctggc
     3001 cggcctgctg cactgcggcg agccggcgtt cctggacgcc aagcacttcc tgatgctgga
     3061 ccagccggcc gccgtcgccg acgccatcgc gcgcaccgcg cacgccgccg cgtgagcccc
     3121 ctgccggacc ggccgggcga cgtggccgtc gccgtggacg tggtggtgct gagcctcgcc
     3181 ggcgagcgcg ccgagccggt gttcctggcc cagccgcgcc ggcgcgaccc gtaccacggc
     3241 gactggtcgc tgcccggcgg catcgtgcgc gagcgggagg acctggaggc ggccgtgcgc
     3301 cgggcgctgg cgcgcgcgac cgccctggag cgcgtgcgcc acctggagca gctcggcacg
     3361 ttcggcgatc cggcacgcga cccgagggga agggtggtgt ccgtgagcta cctggccctg
     3421 ctgcccgagc ccggcccggc gggcgccggg gcgggctggc tgcccgcgac cgcgccgccg
     3481 ccgctggcgt tcgaccacgc cgagatcctg gccgcggcgc tggagcggct gcgcggcaag
     3541 ctgtcctact ccaccgtggc ctacgggctg gtgggcgacg agttcacgct gagcgagctg
     3601 caggcggtgt acgaggcggt gctgggcgag gagctggaca agcgcaactt ccgcaagaag
     3661 gtgctggcgc tgggcgtgct ggaggaggcc gagggcatgc gccggggcgc gcaccggccc
     3721 gcgcggctgt accgcttccg ccaccgcgag ctggtgctgt tcgacgacgt ggtgacccgc
     3781 gcggggacgg cgctgtgagc ggctacggcg ccaccagcgc cctgctcacc gacctgtacg
     3841 agctcaccat gatggccggc tacttcgacg cgggccgcca ccgcgagcag gcgacgttcg
     3901 acctgttctt ccgccggccg ccggacggcg tcgacgtggt cgtcgcctgc ggcctggacc
     3961 cggcgctgga gtacctggcc cagttgcgct tcaccgacgc cgagctggcc tacctgcgcg
     4021 gcctggagct gttctcaccc gcgttcctgg actgcctgcg cgagcttcgc ttcaccggtg
     4081 aggtgtgggc gatgccggag ggcacgccgg tgttccccga cgagccgctg ctgcgcgtga
     4141 gcggcccgct gctcgaggcg caactggtcg agaccgcgct gctcaaccgc gtgtgctacg
     4201 cgaccctggt ggcgtccgtg gccgcccaag tggtggacgc ggccggcggc aaggacgtgc
     4261 tggagttcgg cacgcgccgg gcgcacggcc cggacggcgc gctcaccggc tcgcgcgccg
     4321 ccgtgatcgg cggctgcggg gccacctcca acacggaggc ggggatgcgc ttcggcgtgc
     4381 cggtgtcggg cacgcaggcg cacgcctgga tcatgtcgtt cccgtcggag ctggacgcgt
     4441 tccgctgcta cgccgagacg ttcccggact ccaccgtcct gctggtggac acctacgaca
     4501 ccctgggcat cggcgtgccc aacgcgatca ccgtggcgcg cgagctcgcc gagcgcggcc
     4561 accgactgcg cggcatccgc ctggactccg gcgacctgct ggagctgtcg cgcgcggccc
     4621 gcgcgctgct ggacgaggcc ggcctggccg acgtgcgcat cctggcctcc ggtgacctgg
     4681 acgccgagcg cgtgcgcgcg ctggaggcgg ccggcgcgcc catcgacggc tacggggtgg
     4741 gcaccgcact gctgaccgcc aagcgcgacc cggcgctgtc aggggtgtac aagctggccg
     4801 aggtcggcgg cgaggcggtg ctgaagatct ccagcgacga ggccaagacc accagccccg
     4861 ggcgcaagca ggtgtggcgc gacggcggcg gcgacgtgat cggcctggag ggcgaggacc
     4921 accctggccg gccgctgctg accaacgtca tgcacggcgg gcagcggctg cgtcaccccg
     4981 agcccgtcat gcgcctccgc gagggcagca tggccgaggt
//
LOCUS       DATMKC010000086         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_65950, whole genome shotgun sequence.
ACCESSION   DATMKC010000086 DATMKC010000000
VERSION     DATMKC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65950"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VNU01_02465"
     CDS             <1..380
                     /locus_tag="VNU01_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31516.1"
                     /translation="ISKAYDGQGTIVYADGTVVWLTDVGWKQDVGVMRGKASGQKGKK
                     TDELFVLQTRFGVGDPNCLTKRDNLPPPMGTGPKQLGQGGTAFAHAGWYGVFPFGGQQ
                     NPNDLPFLCKDDVNPVCLYNSKK"
     gene            394..1194
                     /locus_tag="VNU01_02470"
     CDS             394..1194
                     /locus_tag="VNU01_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31517.1"
                     /translation="MLRKGMVIGVGAAVVLTLLAPPAVANKAQPKQPHNEVPNPLPAK
                     DVGLLDGEVKVGKARDGCSNDGKGNVTGGGLGLPGVTTPKNAYYQFTGSVTSLVNRDM
                     ALTEVCGRLTKQHSAVGNGLGAACGASKGWDGKGKVSWEGLPGKQSLWLQDVGWKFTV
                     GGLIFVTGKVVAAGSSAEAKTKQSQDHLLALLLAQGGAECFNKRDAVPPPAGTGEKQE
                     GVGATRFTIIGIYGVLNGPPPDRNAGFQCKFGPEHGCLNRPKKQGGSG"
     gene            complement(1227..2201)
                     /locus_tag="VNU01_02475"
     CDS             complement(1227..2201)
                     /locus_tag="VNU01_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31518.1"
                     /translation="MAAIGVIVALSAITPVRTGLVTVTWRWVASSVGLLGLRATPGEG
                     GLLTFTHGGAAFSYRIVEECTAITVVGLFAVAVLATPSIPWAKRVAGLVAGTVALLAL
                     NWLRLLSLAWVGVHHHELFDLAHVWLWQGVAVAAVIGMWLAWLAWASGRPSGPHWRQA
                     GWFVATAGVMGLLLQATALGSLYSRGVDSVLHLVQRATALLPYRTIQFAEPDSEWGML
                     VRTVSLALAVALTVTLAGDSPRERLRAGLTAVPLVFAAHWAGALLEDASDARLADASA
                     SALTGFVQIPLLVLAHGFTLAVPLLLWARHRREAAEREPAPPFNAVEN"
     gene            2478..>3707
                     /locus_tag="VNU01_02480"
     CDS             2478..>3707
                     /locus_tag="VNU01_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31519.1"
                     /translation="MRIARVIAVLVAVVLSLSGLGAAPAAAHHDFSCAFHDETEFKNL
                     PPGCRNFLIDPGVGNSLTRAKLMAHPELYAKQGINEQFLWHARETVRHVGKDETVSWN
                     RCAYVPGAGNACDPGSVLSSITTIGRQFGGAVTLTVLEHAGMFIGLACGNVRDDVPPP
                     EMPTPGIAGSKFLDRDRDGGWDGDEPGLGGWRIDLHLVESFVGGFAPGKVRETTTASD
                     GGYLFRLDGLPPGRYRVTEESRPGWAQTRQPADVTVPFGIGNDVVKGNDFGNVETRAD
                     VVKASFELIDPPLELRADQPTELLVRATLRNDGPADIVDVTDTLTVGGLEDCAILPRR
                     QTVTRRLVEGQVVVVDLAVTVTCGDPSFHPFDFANDLAVATPGVTDPDPGDNHREFTH
                     TIPVIDLADISVDEVVLD"
BASE COUNT          611 a         1265 c         1310 g          521 t
ORIGIN      
        1 ggatcagcaa ggcctacgac ggccagggca cgatcgtcta cgcggacggg accgtcgtct
       61 ggctgaccga tgtcggctgg aagcaggacg tcggtgtgat gcggggcaag gcgtcggggc
      121 agaagggcaa gaagaccgac gagctgttcg tgcttcagac gcggttcggc gtgggcgacc
      181 ccaactgtct caccaagcgg gacaacctgc caccgccgat ggggacgggc ccgaagcagc
      241 tcggccaggg cgggaccgcg ttcgcccatg ccgggtggta cggcgtgttc ccgttcggcg
      301 gccagcagaa ccccaacgac ctgcccttcc tgtgcaagga cgacgtcaat ccggtctgcc
      361 tctacaactc caagaagtga gaggggagtc gacatgttgc gcaaggggat ggtgatcgga
      421 gtcggcgcgg ccgtcgtgtt gaccctgctg gcgccgcccg cggtcgcgaa caaagcacag
      481 cccaagcagc cgcacaacga ggtgccgaac cctctgccgg ccaaggacgt cggtctgctc
      541 gacggtgagg tgaaggtggg caaggcgcgg gatggctgca gcaacgacgg gaagggcaac
      601 gtgacgggcg gcggtctggg actgcctggc gtgaccacgc cgaagaacgc ctactaccag
      661 ttcacgggga gcgtgacgtc gctcgtcaac cgcgacatgg cgctcacaga ggtgtgcgga
      721 cggctgacga agcagcacag cgcggtcggc aacgggctcg gcgcggcgtg tggggcctcc
      781 aagggctggg acggcaaggg gaaggtgtcc tgggaggggc ttcccggcaa gcagagcctg
      841 tggctgcaag acgtgggctg gaagttcacc gtcggcgggt tgatcttcgt caccggcaag
      901 gtggtcgccg ccggctcgtc ggctgaggcg aagaccaagc agtctcagga ccacttgctc
      961 gcgctgctct tggctcaggg tggcgccgag tgcttcaaca agcgggatgc cgtgccgccc
     1021 cccgcaggca ccggggagaa gcaggaaggc gtcggggcga cacgcttcac catcatcggg
     1081 atctatgggg tgctcaacgg tccgccgcca gaccgcaacg ctggcttcca gtgcaagttc
     1141 ggacccgagc acggctgcct caaccggccg aagaagcagg ggggcagcgg ctgatcgcgc
     1201 aggcccagaa cgtctcaccc gccgaatcag ttttccacgg cattgaaggg tggtgcgggc
     1261 tcgcgttcgg ctgcttcgcg gcggtggcgg gcccacagga ggagcgggac cgccagggtg
     1321 aacccgtgcg ccagcacgag gaggggtatc tgcacgaagc cggtcagcgc ggaggcgctc
     1381 gcgtcggcga ggcgggcgtc gctggcgtcc tccaacaggg cgccggccca gtgggcggcg
     1441 aagacgagcg gcacggcggt cagcccggca cggaggcgct cgcgcgggct gtcgccggcg
     1501 agcgtcacgg tgagtgccac ggccagggcc agtgacacgg tgcggacgag catcccccat
     1561 tcgctgtccg gctcggcgaa ctggatagtc cggtagggaa ggagcgccgt ggcgcgctgc
     1621 acgaggtgca ggacgctgtc gacgccacgg ctgtagagcg atcccagcgc agtggcctgc
     1681 agaagcaggc ccatcacccc cgcggtggcc acgaaccacc ccgcctgccg ccagtggggc
     1741 ccggacggtc ggccgctcgc ccaggccagc caggcgagcc acatgccgat caccgcggcc
     1801 actgccacgc cctgccagag ccacacgtgc gccaggtcga acagctcgtg atggtgcacc
     1861 cccacccacg cgaggctcag caggcgaagc cagttgagcg cgagcagcgc caccgttccc
     1921 gccacgagcc ccgcaacgcg cttcgcccag gggatcgacg gcgtcgcaag caccgcgacg
     1981 gcgaacaagc cgacgacggt gatggcggtg cactcctcga cgatgcggta ggagaacgcg
     2041 gcgccgccgt gcgtgaaagt gagcagccct ccctcgcctg gggtcgccct gagcccgagc
     2101 aatccgaccg atgacgccac ccaccgccac gtcacggtga ccaggccggt gcgcacgggc
     2161 gtgatcgcgc tgagcgccac gatcacgccg atggcggcca tagccagtcg ccaggcgaac
     2221 tcgttcgcgc cgctgcttct caccgccgct tgggcagcag gcccctcccc catggcgccc
     2281 ccgtcggttc ggagcctcac ggttcccacg ggcaagcatg gcccatggcc ctcctgcggt
     2341 caacgcccgc actccaccgc acaatcgggt tccgcacgtt tgtccccgaa ccagacgccg
     2401 ccgtacagtc caccgcggtc ggtggggtgc gggtcccacc cacggcatga cgggagcggg
     2461 tcatggtgcg gagcagtgtg cgaatcgccc gggtgatcgc ggtgctggtg gccgtggtgc
     2521 tttcgctgtc ggggctcggc gcggcccccg cggccgccca tcacgacttt agctgcgcct
     2581 ttcacgatga gaccgagttc aagaacctcc ccccgggttg ccgcaacttc ctcattgatc
     2641 ccggcgtagg caactccctc acccgagcca agttgatggc gcatccggag ctgtacgcca
     2701 agcagggcat caacgagcag ttcctgtggc acgcccgtga gaccgtccgc cacgtcggca
     2761 aggacgagac ggtgtcgtgg aaccgctgcg cctacgtgcc tggcgccggc aacgcgtgcg
     2821 accccggcag cgtgttgtcg tccatcacca cgatcggcag gcagttcggt ggggccgtca
     2881 cgctcaccgt tctcgagcac gccggcatgt tcatcggcct ggcgtgcggc aacgtccgtg
     2941 acgacgtgcc gccgcccgag atgccgactc caggcatcgc gggatcgaag ttcctggatc
     3001 gtgaccgcga cggcggctgg gacggcgacg agcccggctt gggcggctgg cggatcgacc
     3061 tccatctggt cgagtccttc gtcggcgggt tcgctcccgg caaggttcgg gagaccacga
     3121 cggcttccga cggcggctac ctgttccggc tggacggcct gccgccgggg cgctaccgcg
     3181 tgacggagga gtcccggccg ggctgggcgc agacgcgcca gcccgcggac gtgaccgtgc
     3241 ccttcggcat cggcaacgac gtggtgaagg gcaacgactt cggcaacgtc gagacccgtg
     3301 cggacgtggt gaaggcctcc ttcgagttga tcgatccgcc ccttgaactt cgggcggacc
     3361 agccgaccga gctgctggtg cgcgccaccc tgcgcaacga cggtccggcc gacatcgtcg
     3421 acgtgaccga cacgctgacc gtcggcggcc tcgaggactg cgcgatcctg ccgcggcggc
     3481 agaccgtcac tcgccggctg gtggaggggc aggtcgttgt ggtcgacctg gccgtgacgg
     3541 tgacttgcgg cgaccccagc ttccatccgt tcgacttcgc caatgacctc gccgtcgcga
     3601 cgccgggcgt caccgatccc gaccctggcg acaaccaccg cgagttcacg cacaccatcc
     3661 cggtcatcga cctcgccgac atctcggtgg acgaggtcgt cctggac
//
LOCUS       DATMKC010000087         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_329128, whole genome shotgun sequence.
ACCESSION   DATMKC010000087 DATMKC010000000
VERSION     DATMKC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329128"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VNU01_02485"
     CDS             complement(<1..755)
                     /locus_tag="VNU01_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWH31520.1"
                     /translation="MAKRRLPTPSEITEGVALTTLPSGLRIVSEHMPDMRSATVGFWV
                     DAGSRDERDPVAGASHFLEHLLFKGTARRTAQQIAEEIDAVGGDLNAFTSKEYTCFYA
                     RCLDRDLRLAVDVLGDMITSALIRAEDVDAERNVVLEEIRMHYDTPDDLVHSAFSEAW
                     FGPHPLGREILGDLDTITNMKRGQVHGYYRRKYAPRNIVVAAAGNFTHAELVDAVCDV
                     LGDWAGPADVSVPSRKPPRVFPAEPVKVRSRPT"
     gene            complement(761..>2051)
                     /locus_tag="VNU01_02490"
     CDS             complement(761..>2051)
                     /locus_tag="VNU01_02490"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWH31521.1"
                     /translation="YNFPPFSTGETGFMRGPKRREIGHGALAERALLPVIPSGEEFPY
                     TLRLVSEVLSSNGSTSMASVCASTLSLMDAGVPIKAPVAGIAMGLVSEDGKYTTLTDI
                     LGAEDAFGDMDFKVAGTQDFVTALQLDTKLSGIPADVLAGALTQAKEARLAILDVITE
                     CIAEPRAEMRPSAPRVLTEYIPQDKIGEVIGPKGKIIREITDETGADINIDEVDGRGV
                     VHIYSADGEKAQMALDRIRAIANPVVPKEGERYYATVVKTADFGAFMSLTPGSDGLLH
                     ISKLGGGKRLQHADEAVSVGDKIWVEVAEVKDGRKFSLKPVEGPDGGELDRVGTKPGT
                     EAPAGEARQERAGDRAPRGDRGDRGGRGERGGRDRGERRPRTDTPAPEGQRERIRERS
                     DAATDAAPAPEAGAPDAGEGAGGEGEGEGRRRVRRRG"
BASE COUNT          250 a          763 c          717 g          321 t
ORIGIN      
        1 tcggtcgggc ggctgcgcac cttcaccggc tccgcgggga acacgcgcgg cggcttgcgc
       61 gacggcacgg acacgtcggc cggccccgcc cagtcgccca gcacgtcgca gaccgcgtcg
      121 accagctcgg cgtgcgtgaa gttgccggcg gcggccacca cgatgttgcg cggcgcgtac
      181 ttgcgccggt agtagccgtg cacctgcccg cgcttcatgt tcgtgatcgt gtccaggtcg
      241 cccaggatct cgcggcccag cgggtgcggc ccgaaccacg cctcgctgaa cgcgctgtgc
      301 accaggtcgt ccggcgtgtc gtagtgcatg cggatctctt ccagcaccac gttgcgctcg
      361 gcgtccacgt cctcggcgcg gatgagcgcc gaggtgatca tgtcgccgag cacgtccacc
      421 gccaggcgca ggtcgcggtc caggcagcgc gcgtagaagc aggtgtactc cttggaggtg
      481 aacgcgttga ggtcgccgcc caccgcgtcg atctcctcgg cgatttgctg cgccgtgcgc
      541 cgcgccgtgc ccttgaacag caggtgctcc aggaagtggc tggcgccggc caccgggtca
      601 cgctcgtcgc gcgacccggc gtccacccag aagcccaccg tggccgagcg catgtccggc
      661 atgtgctcgg acacgatgcg cagcccgctg ggcagggtgg tcagggccac gccctcggtg
      721 atctccgagg gcgtgggcag acggcgcttg gccatgcggc tcagccgcgg cggcgcacgc
      781 ggcgccggcc ttcgccttca ccctcgccgc cggccccttc accggcgtct ggggcaccag
      841 cctccggggc cggtgcggcg tcggtggcgg cgtcgctgcg ctcgcggatg cgctcgcgct
      901 gaccctcggg ggccggcgtg tccgtgcgcg gccggcgctc gccgcggtca cggccgccac
      961 gctcgccacg gccaccgcgg tcaccgcggt cgccgcgcgg cgcgcggtcg cccgcacgct
     1021 cctgacgagc ttcgcccgcc ggcgcctccg tgcccggctt ggtgccgacg cggtcgagct
     1081 cgccgccgtc cggcccctcg accggcttca gcgagaactt gcggccgtcc ttgacctcgg
     1141 ccacctcgac ccagatcttg tcgccgaccg agaccgcctc gtccgcgtgc tgcaggcgct
     1201 tgccgccacc cagcttcgag atgtgcagca ggccgtcgct gcccggcgtg agggacatga
     1261 aggcgccgaa gtcggcggtc ttcacgaccg tcgcgtagta gcgctcgccc tccttgggca
     1321 ccaccgggtt ggcgatcgcg cggatgcggt ccagcgccat ctgcgccttc tcgccgtccg
     1381 ccgagtagat gtgcaccacg ccgcggccat cgacctcgtc gatgttgatg tcggcgccgg
     1441 tctcgtcggt gatctcgcgg atgatcttgc ccttcggacc gatcacctcg ccgatcttgt
     1501 cctgcgggat gtactccgtg agcacccgcg gggcgctcgg gcgcatctcc gcgcgcggct
     1561 cggcgatgca ctccgtgatg acgtccagga tcgccaggcg ggcctccttg gcctgcgtca
     1621 gcgcgccggc caacacgtcg gccgggatgc cggacagctt ggtgtcaagc tggagcgccg
     1681 tcacgaagtc ctgcgtgccg gcgaccttga agtccatgtc gccgaacgcg tcctccgcgc
     1741 ccaggatgtc cgtcagggtc gtgtacttgc cgtcctcgct gaccaggccc atcgcgatgc
     1801 cggccaccgg cgccttgatc ggcacaccgg cgtccatcag cgacagcgtg gacgcgcaca
     1861 cggaggccat ggaggtggag ccgttggagc tcagcacctc cgacaccagg cgcagcgtgt
     1921 aggggaactc ctcgccgctg gggatcaccg gcagcagcgc gcgctcggcg agcgcgccgt
     1981 ggccgatctc gcgacgcttg gggccgcgca tgaatccggt ctcgcccgtg gagaacggcg
     2041 ggaagttgta g
//
LOCUS       DATMKC010000088         3461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_13510, whole genome shotgun sequence.
ACCESSION   DATMKC010000088 DATMKC010000000
VERSION     DATMKC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13510"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VNU01_02495"
     CDS             <1..752
                     /locus_tag="VNU01_02495"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HWH31522.1"
                     /translation="LELLIAGPAEGDPKDARPALPLSTTIAAFAVDGDTAVVELSQEA
                     VSDAGTVIASPEHELLAMAAVANTLTEFPAIDHVRLSVSGAGREFWGGWGLPELLNRD
                     EAVIGVPREGDPLPPLERFAVEAQRVGSAAAGAVAIGDVRTHDRIGFVRVTVELGDPG
                     GEAPARGVPPTSARLEGDRLVLQIDDVVSSAANPAPGQRLALEGLPFDALEAEAGVLP
                     GPARFALPTGPRPFWLHTLTSPTRVVLDVRK"
     gene            760..3270
                     /gene="gyrA"
                     /locus_tag="VNU01_02500"
     CDS             760..3270
                     /gene="gyrA"
                     /locus_tag="VNU01_02500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWH31523.1"
                     /translation="MSDETPTPDAPAGAVDAPDEVTAQTGRLRVEPIEIEEELQRSYL
                     DYAMSVIVGRALPAVRDGLKPVHRRILFAMFEGGMRAGTQHRKSAAAVGDVMKKYHPH
                     GDSAIYDALVRMAQPWAIRYPLIEGHGNFGSVDGDPAAAMRYTEARLSPLAMELLRDI
                     DEETVDFVENYDGQESEPTVLPSRFPNLLVNGSSGIAVGMATNIPPHNLVEISDAVIA
                     TMANPEITLDELMEIVPGPDFPTGALIMGTQGIRDAYERGRGSIRMRAVSEVEEDDKG
                     VRIVVTELPYQVNKANLAMKIADLVREKRVVGIRDLRDESNRKGTRLVIELKRDANPQ
                     VILNQLYKMTQLQDTFGVINLSLVDGVPRTLPLKDTITHYIAHQVEIITRRTRYRLRK
                     AQERAHVLEGLIVALDHVDEVIALIRAAESAEAALGQLQERYGLSEIQARAILDMQLR
                     RLAGLERQKILDEYAELQRLIAELQGILDDPAKVRAIISDELTELRDKFGDARRTKIV
                     PADGEFDMEDLIAEEDVVVTLTKAGYVKRVKVSEYRTQKRGGKGVSGASLREDDIVRD
                     LFVTTTHHWLLFFTNQGRVYRVKAWQIPEKQRAARGTYVANVEGLALQPDETIAAVVD
                     LKDFEGDRYLVFATRKGIVKRTALKEYDSPRSVLIAINLREGDELIGVTVTSGSDELI
                     LVSRKAMSIRFPETDARPMGRDTSGVIGMDLRADDEVLSMTKCVADGQLLVVTEEGYG
                     KRTPLERYPAQKRGGKGVKTAHLTEERGGLVGALVAAYEQEIFVVTDTGTIIRMDVKD
                     IRPTGRSTQGVRVMKPSEGAKVASIAPVMEGQDLVAEE"
BASE COUNT          569 a         1218 c         1235 g          439 t
ORIGIN      
        1 ccctggagct gctgatcgcc gggccggccg agggcgaccc gaaggacgcg cgccccgcgc
       61 tgccgctgtc gaccaccatc gcggccttcg ccgtggatgg cgacaccgcc gtggtggagc
      121 tgtcccagga ggcggtcagc gacgcgggca cggtgatcgc ctcgccggag cacgagctgc
      181 ttgccatggc cgccgtggcc aacaccctga cggagttccc ggccatcgac cacgtgcggc
      241 tgtcggtgag cggcgcgggc cgcgagttct ggggcggctg gggcctgccg gagctgctca
      301 accgggacga ggcggtgatc ggcgtgcccc gtgagggcga tccgctgccc ccgttggagc
      361 gcttcgccgt cgaggcccag cgggtcggca gcgccgccgc cggcgcggtg gccatcggcg
      421 acgtgcgcac ccatgaccgc atcggcttcg tccgcgtgac cgtggagctc ggcgacccgg
      481 gcggggaggc cccggcgcgc ggcgtgccac ccacgtcggc ccggctggaa ggcgaccggc
      541 tggtgctgca gatcgacgac gtcgtctcca gcgcggccaa tccggcgccg ggccagcgcc
      601 tggcgctgga ggggctgccg ttcgacgccc tggaggcgga ggccggcgtg ctgcccggcc
      661 ccgcgcgctt cgccttgccg acgggcccgc gcccgttctg gctgcacacc ctgaccagtc
      721 ccacacgcgt cgtgctggac gtgcggaagt gaacctgaca tgagcgacga gacccccacc
      781 cctgacgcgc ccgcgggcgc ggtggacgcc cccgacgagg tgaccgccca gaccgggcgg
      841 ctgcgcgtcg agcccatcga gatcgaggag gagctgcagc gcagctacct cgactatgcg
      901 atgagcgtca tcgtcggccg tgcgctgccg gccgtgcgcg acggcctcaa gccggtgcac
      961 cgccggatcc tgttcgcgat gttcgaaggc ggcatgcgcg cgggcacgca gcaccgcaag
     1021 tcggcggccg ccgtcggcga cgtgatgaag aagtaccacc cgcacggcga ctcggcgatc
     1081 tacgacgcgc tggtgcgcat ggcccagccg tgggcgatcc gctacccgct gatcgaaggc
     1141 cacggcaact tcggctccgt ggacggcgac ccggccgcgg ccatgcgcta caccgaggcg
     1201 cgcctgtcgc cgctggcgat ggagctgctg cgcgacatcg acgaggagac ggtcgacttc
     1261 gtcgagaact acgacggcca ggagtccgag ccgacggtcc tgccgtcgcg cttccccaac
     1321 ctgctggtca acggcagctc cggcatcgcc gtgggcatgg cgaccaacat cccgccgcac
     1381 aacctggtgg agatctccga cgcggtcatc gccacgatgg ccaaccccga gatcacgctg
     1441 gacgagctca tggagatcgt gcccggcccc gacttcccga cgggtgcgct gatcatgggc
     1501 acgcagggca tccgcgacgc ctacgagcgc ggccgcggct ccatccgcat gcgcgcggtg
     1561 agcgaggtcg aggaggacga caagggcgtc cgcatcgtcg tgaccgagct gccgtaccag
     1621 gtcaacaagg cgaacctggc gatgaagatc gccgacctgg tgcgcgagaa gcgggtcgtg
     1681 ggcatccgcg acctgcgcga cgagtccaac cgcaagggca cccggctggt catcgagctg
     1741 aagcgcgacg ccaacccgca ggtcatcctg aaccagctct acaagatgac gcagctccaa
     1801 gacaccttcg gcgtgatcaa cctgtcgctg gtggacggcg tcccgcgcac gctgccgctc
     1861 aaggacacga tcacccacta catcgcccac caggtcgaga tcatcacccg ccggacgcgc
     1921 taccgcctgc gaaaggcgca ggagcgggca cacgtcctgg agggcctgat cgtcgccctg
     1981 gaccacgtcg acgaggtgat cgcgctgatc cgcgcggccg agtccgccga ggcggcgctg
     2041 ggccagctcc aggagcgcta cggcctgtcg gagatccagg cgcgggcgat cctggacatg
     2101 cagttgcggc gcctggccgg cctggagcgc cagaagatcc tggacgagta cgccgagctg
     2161 cagcggctga tcgccgagct gcaggggatc ctggacgacc ccgccaaggt gcgcgccatc
     2221 atcagcgacg agctgaccga gctgcgcgac aagttcggcg acgcccgccg gaccaagatc
     2281 gtcccggccg acggcgagtt cgacatggag gacctgatcg ccgaggagga cgtggtcgtc
     2341 acgctcacca aggcgggcta cgtcaagcgc gtgaaggtca gcgagtaccg cacgcagaag
     2401 cgcggcggca agggcgtgtc cggcgcctcg ctgcgcgagg acgacatcgt ccgcgacctg
     2461 ttcgtcacca ccacgcacca ctggctgctg ttcttcacca accagggccg cgtgtaccgc
     2521 gtgaaggcct ggcagatccc ggagaagcag cgcgccgcgc gcggcaccta cgtggccaac
     2581 gtggaaggcc tggcgctgca gccggacgag accatcgcgg cggtggtgga cctcaaggac
     2641 ttcgagggcg accgctacct ggtcttcgcc acccgcaagg gcatcgtgaa gcgcacggcg
     2701 ctcaaggagt acgactcgcc gcgcagcgtg ctgatcgcga tcaacctgcg cgagggcgac
     2761 gagctcatcg gcgtgacggt caccagcggc agcgacgagc tgatcctggt gtcgcgcaag
     2821 gccatgtcga tccgctttcc ggagaccgac gcccggccga tgggccgcga caccagcggc
     2881 gtgatcggca tggacttgcg cgccgacgac gaggtcctgt ccatgaccaa gtgcgtggcc
     2941 gacgggcagc tgctggtggt cacggaggag ggctacggca agcgcacgcc gctggagcgc
     3001 taccccgccc agaagcgcgg cggcaagggc gtgaagacgg cgcacctcac cgaggagcgc
     3061 ggcggcctgg tcggcgccct cgtggccgcc tacgagcagg agatcttcgt ggtcaccgac
     3121 accgggacca tcatccgcat ggacgtcaag gacatccggc ccaccggccg gtcgacacag
     3181 ggcgtgcgtg tcatgaagcc ctccgagggc gccaaggtcg cctcgatcgc gcccgtgatg
     3241 gaagggcagg atctggtcgc cgaggagtag gagggcgcac gacaatggtg tgacggcgcc
     3301 ttggggtccg ctacagtgcc ccggcatgcc gcatgacgtc cccgagacgg cagcaggaga
     3361 gcgcgtgagc gaagcggaac cgggaggcca gcgtcctcaa ggcggcgagc ggcgccgacc
     3421 gtccggatcc gtccctcccg cccgtcgcaa caacgaccgc c
//
LOCUS       DATMKC010000089         6284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_86217, whole genome shotgun sequence.
ACCESSION   DATMKC010000089 DATMKC010000000
VERSION     DATMKC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86217"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VNU01_02505"
     CDS             <1..435
                     /locus_tag="VNU01_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31524.1"
                     /translation="TLASALRPAGEVPETDGPPDTGGPSPTVADGTAASAPPEGFAVE
                     TVGLIRRGEFFPAGPGLVLTRTDRVRIAVRYGGATDGVLLEGHWTVDGAPFRELRVTL
                     SSRQSMHVFTSPVPEDGWPPGLHQVVLTVGGEVVAGIDFRVV"
     gene            523..1038
                     /locus_tag="VNU01_02510"
     CDS             523..1038
                     /locus_tag="VNU01_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWH31525.1"
                     /translation="MGELAVTAVGADRPGIIARVTGVLLEHGGNLEDSSMTILGGQFA
                     IALLVRADSGAAELERALAAATQDLGLLVAVREVDPQGRSAEPTHMVSVYGADRPGIV
                     HAVAETLAEHEVNVTDLTTRVLGDERPVYAMVLEVALPGDVTDAALDAALREAVAGVE
                     VTVRPLDDTAL"
     gene            1038..1553
                     /gene="def"
                     /locus_tag="VNU01_02515"
     CDS             1038..1553
                     /gene="def"
                     /locus_tag="VNU01_02515"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWH31526.1"
                     /translation="MAERPVVGIPEPVLKRPAEPCGGPDAELARDLVDTMRVSPGCVG
                     LAAPQIGVSRRAFCLDLTGHPKVAQPHGLVVLFDPELIVAEGSEVGREGCMSVPDFTA
                     NVRRALRVVVRGMDEQGNERVYESEGFEARAFQHELDHLDGKLILDRAVSNKDVFRRK
                     VYQPPKPKADG"
     gene            1646..3838
                     /locus_tag="VNU01_02520"
     CDS             1646..3838
                     /locus_tag="VNU01_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWH31527.1"
                     /translation="MDTLDLIRIVASLAVIGVFGFFAARRALFLLLLLRKARPMPDRM
                     GNLGEKLRYQLVHVLGQQKILKWTGAGVMHAFVFWGFLVLQSQSLEAIGEVFDPDFHI
                     PLIGRWEFLGFVQDLFTVLVLIGVIGFVLIRLAQNPGRRGRTSRFAGSNLSAGWWVLI
                     LEFSLLYTVMVLRGVRVAEGTLPYEQGAFASRWLGETFLTGLSHDQLEVIATAFLVAH
                     ISVLFGFLLFTLHSKHLHILTIPFNVAFARLPKALGALQADKIDIENMSEDDVLGVGQ
                     IEHFGFKRFLDMYTCTECGRCQSQCPAWNTGKPLSPKLLIMDLRDHLYAKGPYLLDPA
                     KAEKDATAAPLNAKLVGDVPGEDNAVIDFDVLWSCTTCGACVEECPVDIEHVDHILDL
                     RRYKAQMESSFPQEAGAMLRNIENAGDPWGAGQAKRMEWAEGLDVPVVNGQIDPEVEY
                     LFWVGCAGALEDRSKKVTRTVAELLNAAGVKYAVLGQAEACTGDPARRLGMEYLFQMM
                     AQTNVETLKSAGVKKIVAWCPHCFNTLRNEYPDFDGHFEVIHHTQLLSQLVKDGRLAP
                     QTDIEATVTYHDPCYLGRHNEVYDPPRMVVDTIQGLEKVEMGRCRNHGFCCGAGGARF
                     FMEETIGKRVNHERIDEALALNPDFVSTACPFCFVMLDDAVNDKVGSGALGEGQVRVV
                     DVSQLLAQSLLPVASVNGNPVATPAAGSAGPTTDPGESAPVADGPADH"
     gene            3905..4294
                     /locus_tag="VNU01_02525"
     CDS             3905..4294
                     /locus_tag="VNU01_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31528.1"
                     /translation="MAEQEEGRARSAATIVAGLVGLFLVVAGLWAFLAPRPFFDALAV
                     FEPYNAHFIRDIGAFQIGLGAVLLLALRLEDALTVALGGVGAGALFHAAAHVIDRDLG
                     GTPATDIPFFGVMAVVLLAAAFTRRRA"
     gene            complement(4301..4867)
                     /gene="dcd"
                     /locus_tag="VNU01_02530"
     CDS             complement(4301..4867)
                     /gene="dcd"
                     /locus_tag="VNU01_02530"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWH31529.1"
                     /translation="MILSDRSIREQLEAGRVVIDPLGDNAIQPSSVDVRLDDSFRVFA
                     NHRYPYIDVRQPLEDLTELVKATEDDPFVLHPGEFVLGSTFERVTLPDDLVARLEGKS
                     SLGRLGLLIHSTAGFVDAGWDGWLTLELSNVSTLPMALYPGMKIGQLAFFQLTTPAEH
                     PYGSGEVGSKYKGQRGPTPSRYFMNFDR"
     gene            complement(4920..5513)
                     /locus_tag="VNU01_02535"
     CDS             complement(4920..5513)
                     /locus_tag="VNU01_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31530.1"
                     /translation="MTRLNVAFVLPAGLLGCLTMSAALVVGHAEISQLRPHERRMNPV
                     VETVESPFRVIAGVRVPDPAPSHTSVPSPSAATAPASVPLPDDVPVQPPADLLPADTL
                     APPEVAKPGSLRGAVDRQVERTPDAAEPVVAQVVDTVFDAALGAIDLLGLGVPLQQGW
                     RTAEPTYDAAWDQSEPARADAVRAWEDTSAAVQGARS"
     gene            5794..5864
                     /locus_tag="VNU01_02540"
     tRNA            5794..5864
                     /locus_tag="VNU01_02540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5826..5828,aa:Gly,seq:ccc)
     gene            complement(5886..>6284)
                     /locus_tag="VNU01_02545"
     CDS             complement(5886..>6284)
                     /locus_tag="VNU01_02545"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWH31531.1"
                     /translation="TEDHMASPDRAPSPSKLIDARIEELGDWRGEMLARIRRLIKEAE
                     PEVVEEWKWRGVPTWYRDGMLCTGETYKDKVKVTFAKGAALDDRAGVFNASLDGSVRR
                     AIDLRQGDELDEDAFQDLVRAAARLNARSD"
BASE COUNT          915 a         2175 c         2241 g          953 t
ORIGIN      
        1 accctggcga gcgcgctgcg accggccggc gaggtgcccg agacggatgg cccaccggac
       61 acgggaggac cgagcccgac cgtggcggac ggcacggccg cgtcggcgcc acctgaaggc
      121 ttcgccgtgg agaccgtggg cctgatccgc cgcggagagt tcttcccggc cgggccgggg
      181 ctggtgctga cgcgcacgga tcgcgtgcgc atcgccgtgc gctatggcgg cgccaccgac
      241 ggcgtgctgc tggagggcca ctggaccgtc gatggcgcgc cgttccggga gctccgcgtg
      301 acactgtcca gccgtcagag catgcacgtg ttcacctcgc ccgtgcccga ggacggctgg
      361 ccgccgggcc tgcaccaggt ggtgctcacc gtgggcggcg aagtggtggc cggcatcgac
      421 ttccgggtgg tctgaccgcc cgccggcccc ccggggccgc ggctggggcc gtggcctaga
      481 ctcgcgccgg cacttcacca ccaccagcaa ggagcggggc acatgggcga actcgccgtc
      541 accgccgtcg gcgcggaccg gccgggcatc atcgcccggg tgaccggcgt gctcctggag
      601 cacggcggca acctcgagga cagttccatg accatcctgg gcggccagtt cgccatcgcc
      661 ctgctggtgc gcgcggacag cggcgcggcg gagctggagc gcgcactggc ggccgccacc
      721 caggacctgg gcctgctggt ggcggtgcgc gaggtcgacc cgcagggacg cagcgccgag
      781 cccacgcaca tggtctccgt ctacggcgcg gaccggcccg gcatcgtcca cgccgtggcg
      841 gagacgctgg cggagcacga ggtgaacgtc accgatctca ccacgcgcgt cctgggcgac
      901 gagcggcccg tgtacgcgat ggtgctcgag gtcgcgctgc ccggcgacgt caccgacgcc
      961 gcgctggacg ccgcgctgcg agaggcggtg gccggggtcg aggtcaccgt gcgcccgctg
     1021 gacgacacgg cgctgtaatg gccgaacgcc ccgtcgtcgg gatccccgag ccggtcctca
     1081 agcgccccgc cgagccctgc ggtggcccgg acgcggagtt ggcgcgcgac ctcgtggaca
     1141 cgatgcgcgt gtcccccgga tgcgtcgggc tcgccgcccc gcagatcggg gtgagcaggc
     1201 gcgcgttctg cctggacctc accggccatc cgaaggtggc gcagccgcac gggctggtgg
     1261 tgctgttcga cccggagctg atcgtggcgg agggcagcga ggtggggcgc gaaggctgca
     1321 tgagcgtgcc ggacttcacc gcgaacgtgc gccgcgccct gcgcgtggtc gtgcgcggga
     1381 tggacgagca gggcaacgag cgggtctacg agtccgaggg gttcgaggcg cgcgcgttcc
     1441 agcacgagct ggaccacctg gacggcaagc tcatcctgga ccgggcggtg tccaacaagg
     1501 acgtgttccg ccgcaaggtc taccaaccgc ccaagccgaa ggcggacggc tgacgggtcg
     1561 cgcgttcgcg ggccggacag ccggttgact acgatgcccg caccgtcagt gcccccgcat
     1621 gcccggcccc ttcaccgagg gagacatgga cactctggac ctgatccgca tcgtcgcgag
     1681 cctcgcggtg atcggcgtct tcggcttctt cgcggccagg cgcgcgctgt tcctgctcct
     1741 gctgctgcgc aaggcgcggc ccatgcccga ccgcatgggc aacctgggtg agaagctgcg
     1801 ctatcagctc gtccacgtgc tgggccagca gaagatcctg aagtggaccg gcgcgggcgt
     1861 catgcacgcc ttcgtgttct ggggcttcct ggtcctgcag agccagtccc tggaggccat
     1921 cggcgaggtc ttcgaccccg acttccacat cccgctgatc ggccgctggg agttcctggg
     1981 cttcgtccag gacctgttca cggtgctggt gctgatcggc gtcatcggct tcgtgctgat
     2041 ccgcctggcg cagaacccgg gacgccgcgg gcgcacgtcg cgcttcgccg gctccaacct
     2101 cagcgccggc tggtgggtgc tgatcctgga gttcagcctg ctctacacgg tgatggtgct
     2161 gcgcggcgtg cgcgtggcgg agggcacgct gccctacgag cagggcgcgt tcgcctcgcg
     2221 ctggcttggc gagacgttcc tcaccgggct gtcgcacgac cagctggagg tcatcgccac
     2281 ggcgttcctg gtggcccaca tctccgtgct gttcggcttc ctgctgttca cgctgcacag
     2341 caagcacctg cacatcctga cgatcccgtt caacgtggcg ttcgcgcgcc tgccgaaggc
     2401 cctgggcgcg ctgcaggccg acaagatcga catcgagaac atgtccgagg acgacgtgct
     2461 gggtgtcggc cagatcgagc acttcggctt caagcgcttc ctggacatgt acacgtgcac
     2521 cgagtgcggc cgctgccaga gccagtgccc cgcgtggaac accggcaagc cgctgtcgcc
     2581 gaagcttttg atcatggacc tgcgcgacca cctctacgcc aagggcccgt acctgctgga
     2641 ccccgccaag gcggagaagg acgcgaccgc ggccccgctg aacgccaagc tggtcggcga
     2701 cgtcccgggc gaggacaacg cggtcatcga cttcgatgtg ctgtggagct gcaccacgtg
     2761 cggcgcgtgc gtggaggagt gcccggtcga catcgagcac gtcgaccaca tcctggacct
     2821 gcgccgctac aaggcgcaga tggagtcgag cttcccgcag gaggccggcg cgatgctgcg
     2881 caacatcgag aacgccggcg acccgtgggg cgccggccag gccaagcgca tggagtgggc
     2941 cgagggcctg gacgtgccgg tggtcaacgg ccagatcgac ccggaggtgg agtacctgtt
     3001 ctgggtgggc tgcgcgggcg cgctggagga ccgctccaag aaggtcacgc gcacggtggc
     3061 ggagctgctc aacgccgccg gggtgaagta cgcggtgctg ggccaggcgg aggcctgcac
     3121 gggcgacccc gcgcggcgcc tgggcatgga gtacctgttc cagatgatgg cccagaccaa
     3181 cgtcgagacg ctgaagtcgg cgggcgtcaa gaagatcgtg gcgtggtgcc cgcactgctt
     3241 caacacgctg cgcaacgagt accccgactt cgacgggcac ttcgaggtga tccaccacac
     3301 gcagttgctg agccagctcg tgaaggacgg ccgcctggcg ccgcagaccg acatcgaggc
     3361 cacggtcacc taccacgacc cgtgctacct ggggcggcac aacgaggtct acgacccgcc
     3421 gcgcatggtg gtggacacca tccagggcct ggagaaggtg gagatgggcc gctgccgcaa
     3481 ccacggcttc tgctgtggcg ccggcggcgc gcgcttcttc atggaggaga ccatcggcaa
     3541 gcgcgtcaac cacgagcgca tcgacgaggc gctggcgctg aacccggact tcgtgtccac
     3601 ggcctgcccg ttctgcttcg tgatgctgga cgacgcggtg aacgacaagg tcggctccgg
     3661 cgcgctcggt gagggccagg tgagggtggt cgacgtgagc cagctcctgg cgcagtcgct
     3721 gttgccggtg gcctcggtca acggcaaccc ggtcgccacg ccggccgccg gctccgccgg
     3781 ccccaccacg gacccgggcg agtccgcgcc cgtcgccgac ggcccggccg accactagcc
     3841 tccaccacct tgggacgggc cggacggata ctcgtccggt ccgttctcgt tccaaggagg
     3901 ggccatggca gagcaggagg aagggcgcgc gcggtcggcg gcgacgatcg tggcggggct
     3961 ggtggggctg ttcctcgtgg tggcgggact gtgggcgttc ctggccccgc ggccgttctt
     4021 tgacgcgctc gcggtgttcg agccctacaa cgcccacttc atccgtgaca tcggcgcgtt
     4081 ccagatcggg ctgggcgctg tgctgctgct ggcgctgcgc ctggaagacg cgctcacggt
     4141 ggcgctgggc ggcgtgggcg ccggcgcgct gttccacgcg gcggcgcacg tcatcgaccg
     4201 agacctcggg ggcacgcccg cgaccgacat cccgttcttc ggcgtgatgg cggtggtgct
     4261 gctcgccgcc gcgttcaccc gccgccgggc ctgagcggtc ctagcggtcg aagttcatga
     4321 aatagcggct gggggtgggc ccgcgctggc ccttgtactt ggaacccacc tcgccgctgc
     4381 cgtacggatg ctccgccggg gtcgtgagct ggaagaacgc gagctggccg atcttcatcc
     4441 cgggatacag cgccatcggc agcgtggaga cgttggacag ctccagtgtc agccagccgt
     4501 cccatccggc gtccacgaag cccgcggtgg agtggatgag caggccaagg cggcccaggc
     4561 tggacttgcc ctccagccgc gccaccaggt cgtcgggcag cgtgacccgc tcgaatgtgc
     4621 tgcccagcac gaactcgccg gggtgcagga cgaaggggtc gtcctcggtg gccttcacca
     4681 actccgtgag gtcctccagg ggctggcgga cgtcgatgta ggggtagcgg tgattggcga
     4741 acacccggaa gctgtcgtcc aggcgcacat cgacgctcga cggctggatc gcgttgtcgc
     4801 ccagcgggtc gatgacgacg cggccggcct cgagctgctc gcggatggag cggtcggaca
     4861 ggatcatggg gccctcgctg cctggtgtcg gacagcgcaa gggtagagca ccgcgaggtc
     4921 taggagcgtg ccccctgcac cgcggccgac gtgtcctccc aggctcgcac ggcgtccgcg
     4981 cgagccggct ccgactgatc ccaggccgcg tcgtaggtgg gctccgcggt ccgccagccc
     5041 tgctgcagcg gcacgcccaa gcccagcagg tcgatcgcgc cgagggcggc atcgaacacc
     5101 gtgtcgacca cctgagcgac caccggttcc gccgcgtccg gcgtgcgctc cacctggcgg
     5161 tccacagccc cgcgcaacga accgggcttg gccacctcag ggggcgcgag cgtgtcggca
     5221 ggaagaaggt ccgccggcgg ctggacggga acgtcgtctg ggagaggcac cgatgccggc
     5281 gccgtcgctg ccgaggggga tgggacggac gtatgcgacg gcgccgggtc cggcacgcgt
     5341 actccggcga tcaccctgaa gggcgattcg acggtctcga cgaccgggtt catacggcgc
     5401 tcatgcggac ggagctgaga gatctcggcg tgccccacca ccagcgccgc ggacatcgtg
     5461 agacatccga gcaaccccgc tgggaggaca aacgcgacgt tcagtcgcgt catgccggct
     5521 cctttacccg accgatgaga ctgccaccgt acgcaggacg taggagcact gtcaagggaa
     5581 ttccgtacgc catagtacgt acgtctcata cgtagaccac ccgccaccgt cgcgcccgcc
     5641 gcgcgacctc tcaagggaga tcttcggcac gtcggggcca gaacttcact cgatcggggg
     5701 atggtcgcag tcccccggct gggcgacacc gtcacggcgc tgcccagccg attgcagggg
     5761 ccgtccgcgc gcgcctatca ttcgcgagcc tccgcgggtg tagttcaatg gtagaacacg
     5821 agcttcccaa gctcggaacg ggggttcgat tcccctcacc cgctctccgc tccttggtgt
     5881 ccgggtcagt cagacctggc gttcaggcgc gcggccgcgc gcacgaggtc ctggaacgcg
     5941 tcctcgtcca gttcgtcccc ctgtcgcagg tcgatcgcgc gcctgacgct cccgtccagg
     6001 ctggcgttga acacgcccgc gcggtcgtcg agcgctgcac ccttggcgaa ggtgaccttc
     6061 accttgtcct tgtaggtctc tcccgtgcac agcatcccgt cgcggtacca cgtcggcact
     6121 ccgcgccact tccactcctc cacgacctct ggctccgcct ctttgatgag ccggcggatg
     6181 cgggcgagca tctccccgcg ccagtcaccg agttcctcga tcctggcgtc gatcagcttg
     6241 gaaggggacg gggctcggtc ggggctcgcc atgtggtcct ctgt
//
LOCUS       DATMKC010000090         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_328741, whole genome shotgun sequence.
ACCESSION   DATMKC010000090 DATMKC010000000
VERSION     DATMKC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328741"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2049)
                     /locus_tag="VNU01_02550"
     CDS             complement(<1..2049)
                     /locus_tag="VNU01_02550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWH31532.1"
                     /translation="MEAPFTERSMRRIRAAGVVLAVVELSLVPHPPAAAWLVESLLVA
                     VPLMLLGANAVSLWAPSWRLGPAAQIVLDAGVALSVIGGLGFEPQGSLWVLLALPIAE
                     GALRWGMGGVVRVWAGVLPLHALVWDWGYARFGVDPLDLESVGYRSGMLLLLGSITGV
                     LSGHLGRQAAELQRALRESERRAELLRTVAATGRRLPVEDLGSLLDAVAGSAMALGFD
                     AAELALVDPRTEQRITAAHRGLPEEAAQPRPIDRGLVGEVWRTGRTVVIEDYSSWAGA
                     DPQVLRVGLRQAIGVPVWVSGAVEGVLCAGWLRSRTVGSDEMECLEMLAVEAGAALAA
                     SRRLEDQQAYAEQLRRQARRDALTGLANRGQLLEVVRARAAEVPAALLFVDLDRFKHV
                     NDSYGHAGGDQLLRAVSARMGGAVRPPDVIARFGGDEFVVLLQEADEIAAVRVAERLH
                     ETLREPFTVFNRPVRVSASIGVALLPPGGDPHLALVQADMAMYAAKQSRKRRTAVHDE
                     RLHREALRRVRIEVDLHGVEERGELSVAYQPLCLPDGRGVVGAEALLRWTHPELGVVG
                     PSEFVPMAERIDCLRPIGWWLVATACEQLARWRERDPSFCLWLNVSPGQLTEPGFVPR
                     LSMTLGRLRLPGSALVLELTESPLLAEDVAAARAAMADLHQLGVRMVADDFGQESSSL
                     G"
BASE COUNT          286 a          847 c          746 g          210 t
ORIGIN      
        1 ccccagggac gaggactcct ggccgaagtc gtcggcgacc atgcgcacgc cgagctggtg
       61 caggtccgcc atggcggccc gcgccgccgc gacgtcctcg gccagcagcg gcgactcggt
      121 cagctccagg accagggcgg agcccggcag gcgcaggcgc cccagcgtca tggacagccg
      181 gggcacgaac ccgggctcgg tgagctgccc cgggctgacg ttgagccaca ggcagaacga
      241 cgggtcccgc tcgcgccagc gcgccagctg ctcgcacgcc gtggcgacga gccaccagcc
      301 gatcggccgg aggcagtcga tgcgctcggc catcggcacg aactccgaag ggcccaccac
      361 acccagctcc gggtgcgtcc agcgcagcag cgcctcggcg ccgaccacgc cacggccgtc
      421 cggcaggcac agcggctggt aggccacgga cagctcgccg cgctcctcca cgccgtgcag
      481 gtccacctcg atgcgcacgc gccgcagcgc ctcgcggtgc aggcgctcgt cgtgcacggc
      541 cgtgcgccgc ttgcggctct gcttggcggc gtacatcgcc atgtcggcct ggaccagcgc
      601 caggtggggg tcgccgcccg gcggcagcag cgccaccccg atgctcgcgc tgacccgcac
      661 gggccggttg aacacggtga acggctcccg cagcgtctcg tgcaggcgct cggccacccg
      721 taccgccgcg atctcgtcgg cctcctggag caggaccacg aactcgtcgc cgccgaagcg
      781 cgcgatcacg tcgggcggcc gcacggcgcc gcccatgcgc gcggacacgg cgcgcaggag
      841 ctggtcgccg ccggcgtggc cgtagctgtc gttgacgtgc ttgaagcggt cgaggtccac
      901 gaacagcagc gcggccggca cctcggcggc gcgggcgcgc accacctcca ggagctgccc
      961 gcggttggcc aggcccgtga gcgcgtcgcg gcgcgcctgg cggcgcagct gctcggcgta
     1021 ggcctgctgg tcctccagcc gccgggacgc cgccagcgcc gcccccgcct ccacggcaag
     1081 catctccagg cactccatct cgtcggagcc gacggtgcgg ctgcgcagcc agcccgcgca
     1141 cagcacgccc tccacggcgc cggacaccca caccggcacg ccgatggcct ggcgcaggcc
     1201 cacgcgcagg acctgggggt cggcgccggc ccacgagctg tagtcctcga tcaccacggt
     1261 gcgcccggtg cgccacacct cgcccaccag gccgcggtcg atgggccgcg gctgcgccgc
     1321 ctcttcgggc aggccgcggt gcgcggcggt gatgcgctgc tcggtgcgcg ggtccaccag
     1381 cgcgagctcg gccgcgtcga agcccagcgc catggcgctg ccggcgacgg cgtccagcag
     1441 cgagccgagg tcctcgacgg gcagccgccg gccggtggcc gcgaccgtgc gcagcagctc
     1501 cgcgcggcgc tccgactcgc gcagggcgcg ctgcagctcc gccgcctgcc ggcccaggtg
     1561 cccgctcagc acaccggtga tcgagccgag cagcaacagc atgccgctgc ggtagccgac
     1621 cgactccagg tccagcgggt cgacaccgaa gcgcgcgtag ccccagtccc acacgagcgc
     1681 gtgcagcggg agcacgccgg cccacacgcg cacgacgccg cccatgcccc agcgcagcgc
     1741 gccctcggcg atgggcagcg ccagcagcac ccacagggag ccctgcggct cgaagcccag
     1801 gccgccgatg accgacaggg cgaccccggc gtccaggacg atctgggcgg ccggccccag
     1861 ccgccaggac ggcgcccaca ggctcacggc gttggcgccc agcagcatga gcgggacggc
     1921 cacgagcagc gactcgacga gccacgcggc cgcgggcggg tgcgggacca ggctgagctc
     1981 gacgacggcc agcacgacgc ccgcggcgcg gattcgccgc atcgaccgtt cggtgaacgg
     2041 tgcctccatg ctcccgcctc gctccgcccg gcccccgtcc gccgccgtc
//
LOCUS       DATMKC010000091        17501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_303178, whole genome shotgun sequence.
ACCESSION   DATMKC010000091 DATMKC010000000
VERSION     DATMKC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 17501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17501
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303178"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VNU01_02555"
     CDS             complement(<1..820)
                     /locus_tag="VNU01_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWH31533.1"
                     /translation="MASHDKDILTVSGIPTRGVYTPADTPGLADAYGDKLGEPGKFPF
                     TRGVYGTMYRGRPWTMRQYAGFATAAESNARYKYLLSQGQTGLSVAFDLPTQMGYDSD
                     ADIAAGEVGKVGVAIDSLADMRILFDGIPLDQVSTSMTINAPASLLLLLYQLVGEEQG
                     VSPSELRGTIQNDVLKEYIARGTYIFPPEPSLRIITDTFSYCAQEMPNFNTISISGYH
                     MGEGGATPVQEIAFTIANGIAYVQAAVDAGLDVDQFAPRLSFFWVARSTLLEEVA"
     gene            875..1684
                     /locus_tag="VNU01_02560"
     CDS             875..1684
                     /locus_tag="VNU01_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31534.1"
                     /translation="MGQTSGFQVQGSAPEHYDRHVAVFMAPMVRALVERSGLSAGGAL
                     LDVACGTGFVARAAAPVVGPEGRLAGADVNPAMLEHARAVCAGVVPPISFHHAPAESM
                     PFGEAEFDAVLCQQGLQFFPDVDAATAEAARVLRPGGRYAATVWTALDDSPYFVAQCQ
                     AVRALAGDDDADAFASAFSFPPDRLTGALTNAGLRDVEVETIAPVVELPPLEDFVPGH
                     FGAIPWGGTIAEQPDGLATATRMIAEALGDRVRADGSVAVPFGVLVVSGTR"
     gene            1681..2490
                     /locus_tag="VNU01_02565"
     CDS             1681..2490
                     /locus_tag="VNU01_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31535.1"
                     /translation="MTGASGFQLRAGGPASYERYNAVFMAPLVAALLERSGLGTGGAL
                     LDLACGPGLIARAAAAIVGPQGRLDGVDVNGPMIAHARAASSGTVPSITYHEAPAEAL
                     PLADAEFDAVLCQQGMQFFTDLDAAVAECARVLRPGGRLAATVWSALEESPYMQAHSL
                     ALARLEGPEEGAAVAAPFSFPPERLTAAFEQAGLRNVAVERVSPEVELPALEEFAPGH
                     FAGVPWGRRTSADPVQTAKAVALMKEHLRRWTRPDGRVVVPFSSVLVSGVR"
     gene            2593..2988
                     /gene="sdhC"
                     /locus_tag="VNU01_02570"
     CDS             2593..2988
                     /gene="sdhC"
                     /locus_tag="VNU01_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWH31536.1"
                     /translation="MATSTAHAPARKSFYRGGEGMFTWVLHRVTGVLIFVFLFAHIVD
                     TSLIVVSPQLYDGVIEVYHHPLIKLMEIGLVVAVLYHACNGIRVTLVDFWSKGAQRNR
                     GMMRVQRVVFVALSAGAAIPMTLQLINDL"
     gene            3002..3427
                     /locus_tag="VNU01_02575"
     CDS             3002..3427
                     /locus_tag="VNU01_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HWH31537.1"
                     /translation="MTQLNTAENRSRAMQPARQRTSSNTELYSWIFMRVSGILLVLLL
                     FGHLWIMHLIEGGVSRVNFAFVAGRLASPFWQTYDWALLFLATIHGMNGMRIIISDYV
                     RTPQKRFWAKMALYATAGFMLLLGTLVIVTFDPALPLEG"
     gene            3441..5183
                     /gene="sdhA"
                     /locus_tag="VNU01_02580"
     CDS             3441..5183
                     /gene="sdhA"
                     /locus_tag="VNU01_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWH31538.1"
                     /translation="MIHTHTYDVVIVGAGGAGLRAAIEAAGKVRTAVITKLYPTRSHT
                     GAAQGGMCAALGNLEEDKWEWHAFDTVKGSDYLGDQDAIDVMCKEAVDAVIELEHMGL
                     PFSRNDDGTIAQRPFGGHTRNHGEAPVRRACYAADRTGHMILQTLYQQCVKRDVEFFN
                     EFYVLDFLKSPDGTQTAGVVAYELRTGDLHVFRAKSVVFASGGFGKMFKVTSNAHALT
                     GDGPAVLYRRGFPLEDMEFFQFHPTGIYRLGILLSEAARGEGGILRNSDGERFMERYA
                     PTIKDLAPRDMVSRAIRQEINEGRGVGPNGDYVLLDLTHLPKEQLDEKLPDITEFART
                     YLGVEPYSEGVPIQPTAHYAMGGIPTNVNAQVRTGAGETFPGLYAAGECASVSVHGAN
                     RLGTNSLLDIVVFGRRAGVAAVEHALSTRDVADLPENPTADVEAQLERIRSNTTGESA
                     VKIREELQQSMNDNAQVFRTGELLAEQQRILQGLQQRYDGIRIDDKGKSFNTDLVEAV
                     ELGHLLDLAEALVDGAMTREESRGAHYREDFPKRDDANWLKHTLVYRNPDGSRRFDYR
                     PVTMGRYEPMERKY"
     gene            5219..5917
                     /locus_tag="VNU01_02585"
     CDS             5219..5917
                     /locus_tag="VNU01_02585"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWH31539.1"
                     /translation="MEATLRVLRYNPEGDTEPHFEEYKVPVDPMDRVLDALHHIKWNF
                     DGSLSFRRSCAHGICGSDAMLINGRNRLACKVLMKDLPGTVTVEPIKGLRILKDLIVD
                     MDAFFESYKSVLPFLINDEPTGDKERLQSQEDRERFDDTTKCILCASCTTSCPIYWPD
                     NSYVGPASIVNAHRFIFDSRDRGAAERLDKLAGADGVWKCRTTFNCTNACPRDIDVTT
                     AIQQVKRELIFNRV"
     gene            5917..6333
                     /locus_tag="VNU01_02590"
     CDS             5917..6333
                     /locus_tag="VNU01_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH31540.1"
                     /translation="MEQRVSLITLGVADVHRAWRFYEALGWTGETPDDDVVFFQAGGM
                     VVALWSREKLAEDSVVTDPGGWGGVTLAYCTRSREEVDEILRTAEAGGARIGRWGAET
                     FWGGYSGIFVDPDGHPWEVAHNPGWIVTEDGRTLLR"
     gene            complement(6349..6762)
                     /locus_tag="VNU01_02595"
     CDS             complement(6349..6762)
                     /locus_tag="VNU01_02595"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HWH31541.1"
                     /translation="MEPVQIDPLAVLVAAVAHFVLGGLWYSPLLFAKPFTRAVGKTEE
                     ELREGSSPLMYVWAFLGGLAKSVGLAVVLGWIADDLGVAEAAGVGLLIGVLIHGAADA
                     VNAFFESRNRTLVAISVAYDAAGLAIAGAIIAAMS"
     gene            6825..7283
                     /locus_tag="VNU01_02600"
     CDS             6825..7283
                     /locus_tag="VNU01_02600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH31542.1"
                     /translation="MTDHIMRELAADEAHLAAAALLALRPHFAGEDAITAAVRRQMAQ
                     GYRLLAAFAPGDPAAAAVAGFRVVEYLAWGRALYVDDLSTREEHRRAGHARALLDELM
                     ALAEREGCAQFHLDSGFGASRANAHRLYYEVGLYPTSLHFSSRVPPTPRG"
     gene            complement(7280..8470)
                     /locus_tag="VNU01_02605"
     CDS             complement(7280..8470)
                     /locus_tag="VNU01_02605"
                     /inference="COORDINATES: protein motif:HMM:NF006102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HWH31543.1"
                     /translation="MEKVFVVGVGMTKFEKPGAKEWDYPDMAKEAGTKALADAGIDYR
                     LVQQAMVGYVYGDSTAGQRAVYELGLTGVPVVNVNNNCSTGSTALWLAAQHIRGGLAD
                     CVLALGFEKMEKGSLGAKFTDRTLPLDKHYMKMFELREPAQSPPAPQIFGNAGREHME
                     RYGSTAEMFAAVGEKNHRHSANNPYAQFQDVYSLEQILSSPMIHDPLTKLQCSPTSDG
                     SGAAVLASERFVREHGLEDKAVEILGQSMVTDMESTFSTNSVISLVGADMTREAARQV
                     YEQSGLGPEDLDVIELHDCFSANEIITYEALGIAPEGKGGELALAGATTYGGDVVVNP
                     SGGLISKGHPLGATGLAQCAELNWQLRGQADARQVDGARVALQHNIGLGGAVVVTMYR
                     KAAL"
     gene            8600..9853
                     /locus_tag="VNU01_02610"
     CDS             8600..9853
                     /locus_tag="VNU01_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HWH31544.1"
                     /translation="MDEPRDDMTPFEAVNWFFHRAADRVGLDDQTRAVLAGTYREIRV
                     QVALRRDDGSVQTVYGYRVQHNGARGPYKGGVRFHPEADLEEVRALASLMTWKTAVVE
                     LPFGGAKGGVQVDPKQLSLPELERLTRRYMSQVSYVVGVNRDIMAPDVNTNAQTMAWM
                     MDAYGQRKGHTPGIVTGKPVELGGSLGREAATGRGVVMVTNEMVAAAGKSPEQMTMAI
                     QGFGNVGSWTARVGAADGYKVVAVSDVNGGIYAAHGLPVADLLQHIEETGTVVGYPGC
                     DALTNEELLELDVDVLVPAALGGVITKANADAIKARWVVEGANHPVTPAADGVLAGNG
                     ITVVPDILANAGGVTVSYFEWTQNIQQFAWDEERVNTELGKVMREAVRQVRDRSVADG
                     TSLRDAAFSIAVERVAQAAHLRGYV"
     gene            complement(9844..10416)
                     /gene="orn"
                     /locus_tag="VNU01_02615"
     CDS             complement(9844..10416)
                     /gene="orn"
                     /locus_tag="VNU01_02615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HWH31545.1"
                     /translation="MTEALKDPIVWVDLEMTGLDPETDVVLEIAVIVTDGSLETVIEG
                     PDLVLYATDEELQRMPPVVVEMHGVSGLTKAVRDSKIDVREAQREALAFIRQHVPEAR
                     TAPLAGNSIGTDRMFLRKHMPELEAHLHYRNIDVSTLKELVGRWYPGALAQRPEKNGG
                     HRALADIRESIAELRWYREHIFRKPEAPQT"
     gene            complement(10431..10850)
                     /locus_tag="VNU01_02620"
     CDS             complement(10431..10850)
                     /locus_tag="VNU01_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWH31546.1"
                     /translation="MIAYFDSSAFVPLLIDEPASAAALDLWSDADRVVSVPLLYVETR
                     AALGRARRLSRLAGYSYQSTLALFELLHRNVAQARIDEELIRRAGELADAQALRGYDA
                     MHLATAERLADDDVLFVSGDRALCAAARALGLQVSEL"
     gene            complement(10847..11146)
                     /locus_tag="VNU01_02625"
     CDS             complement(10847..11146)
                     /locus_tag="VNU01_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWH31547.1"
                     /translation="MVMHNVAPSKGQRMEVGIRELRNHLSRYLDEVRQGEEIVVTDRG
                     RAVARLVPLSGERKIDRLIAEGLVTPAKNRGPRTVPKPIKIDGTVSDLIIEERRW"
     gene            complement(11171..12949)
                     /locus_tag="VNU01_02630"
     CDS             complement(11171..12949)
                     /locus_tag="VNU01_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWH31548.1"
                     /translation="MNLVSLERVSKRHAEKQVLSEVTLGIDDGDRVGVIGVNGSGKST
                     LLRLIAGAEEPDDGRVVLANDARVHYLPQEPGLDPADTPLQAVLRGIEAPQHRAEEML
                     DRLGVRDWDRPVSERSGGERKRVALARALADEANVLILDEPTNHLDVDVIEWLEGRLL
                     GWQGALILVTHDRYLLDDVATRIVEVERGGIHTGYGNYADYLQTRAVREEQAAAEERK
                     RANRTRTELEWLRRGPKARTSKAKYRVERAKALVEFRAEERRRDVEIGLPSRRIGSKV
                     VNLHNVGKRFADGAGERWVLRGVEHKLAPDARIGLVGPNGSGKSTLLRLIVGELEPDE
                     GKVVVGETIVPGFYRQEPTPMPNTRVIDALKEVVLETNTVEGITLSASQLLERFLFTG
                     EAQKAYVEELSGGERRRLELLRVLATAPNLLLLDEPTNDLDLDTLRVLEEYLDGWQGA
                     LVVASHDRYFLDRVCDDIWSVQPDGSLRHHPGGWSAYQAAQEQEQERAAAAASQPLST
                     GEQPRGERRRRKLGYNETRELALLSERIPQLEARRAQLERAVQEAGADYEAAAEAGDE
                     LATLLAELDAAETRWLELSEFADDGT"
     gene            complement(12969..13952)
                     /gene="trpS"
                     /locus_tag="VNU01_02635"
     CDS             complement(12969..13952)
                     /gene="trpS"
                     /locus_tag="VNU01_02635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWH31549.1"
                     /translation="MAIVFSGMQPTGEVHLGNYLGAVSRWVRDQAPGQYFCVVDLHAI
                     TIPIPPAELRARTVDLATWWLAAGLDPEVCTVFVQSQVREHTELAWILNCVAGMGELG
                     RMTQFKDKAEGKDFVSVGLFDYPVLQAADILLYGTQEVPVGADQRQHIELARDIAQRF
                     NSRFGDTFTLPKAVFPEAGARIMDLQHPDKKMSKSADSPQGTILLAEEDTATRKKVMR
                     AVTDSGSEVRAEPLPDKQGIANLLDMMAAVTGRTVAQLERDYDGRGYGDFKKDLAEAV
                     NEFLRPVRERYQSLKDDRGAVEEALRKGADKAREVAAATMAQVRERVGLSA"
     gene            complement(14078..16033)
                     /gene="acs"
                     /locus_tag="VNU01_02640"
     CDS             complement(14078..16033)
                     /gene="acs"
                     /locus_tag="VNU01_02640"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWH31550.1"
                     /translation="MTDAAIENLMREGRTFPPSPEFTAQANVSDASIYEQAERDPEGW
                     WVSQAERLRWMEPWHTVLDWKPPHAKWFLGGKLNVSDNCLDRHVEAGHGGQVAYHWEG
                     EPGEQRAITYAELKDEVSRFANALKSMGVKKGDPVTIYLPMIPELPVAMLACARIGAP
                     HSVVFAGFSSQALVDRIEDAKARFLITADGSYRKGAVVKLKDAADAAMEQTDLIERCV
                     VVRRTGEDVGFTTGRDVWWHEAVAGQPTECPPEPMDAEDTLFILYTSGSTGKPKGIQH
                     TTGGYLTHVAATHQLVFDLKPDTDIYWCAADIGWVTGHSYIVYGPLANRATSILYEGA
                     PDFPDKDRWWEMIARYKATILYTAPTAIRTFMKWGTELPARHDLSSLRLLGSVGEPIN
                     PEAWVWYHTTIGGGRCPVVDTWWQTETGGIMITPLPGVTAAKPGSAMTPFPGVKAVVV
                     DQEGNPVPPGGGGYLTLQRPWPGMLRTLWGDDQRYVDTYWSRFGTEVYFAGDGAKLDD
                     DGHIWLLGRVDDVMNVSGHRISTTEVESALVGHPKVAEAAVVGRQDAMTGQAIVAYVT
                     LKGGNEPGDELAAELREHVGTEIGKIARPANILFTDDLPKTRSGKIMRRLLRDIADGR
                     ELGDVTTLANADIVSEIAARGATTRED"
     gene            complement(16212..16472)
                     /locus_tag="VNU01_02645"
     CDS             complement(16212..16472)
                     /locus_tag="VNU01_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31551.1"
                     /translation="MPHRNRPGRPEGAQTAGQRVRSRVMARPEVELSDEELRALADDG
                     IDPDDLRRIARERSDALEASDHDRDTVRDELEGLTLEAEDDY"
     gene            complement(16518..>17501)
                     /gene="mdh"
                     /locus_tag="VNU01_02650"
     CDS             complement(16518..>17501)
                     /gene="mdh"
                     /locus_tag="VNU01_02650"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWH31552.1"
                     /translation="AVGSAATSLSRIVSKECPVKITVVGAGKYGSTTVQRIAEAGYAD
                     EVVMTDIVEGLPQGLALDMNQSRPIERFRTKVIGTNDYAATAGSDIVVVTAGKPRKPG
                     MSRMDLLKDNAQIVGGVAKEVARTSPDAVVIVVSNPLDEMTALFQKVSGFPSARVMGQ
                     AGMLDSARFADKTAEKLGVSPLAVEAITLGSHGETMVPVPSKVTVDGKPLRDLLSADE
                     IEQIVQATRDGGAEVVALLKTGSAYYAPSSAAAKMVRAIAEDTKDVMPVCALVTGEYG
                     ISDVYLGVPARIGRGGVEEIVELDLEPQELDALKTAAEAVREKQSDVDSLV"
BASE COUNT         2461 a         6246 c         6090 g         2704 t
ORIGIN      
        1 tcgccacctc ctccagcagc gtggaacggg cgacccagaa gaagctcagc cgcggcgcga
       61 actgatccac gtccaggccg gcgtccacgg cggcctgcac gtaggcgatg ccgttggcga
      121 tggtgaaggc gatctcctgg accggcgtcg ccccgccctc gcccatgtgg tagccgctga
      181 tggagatggt attgaagttg ggcatctcct gggcgcagta ggagaacgtg tcggtgatga
      241 tcctcaagga aggctcgggc gggaagatgt aggtgccgcg cgcgatgtac tccttgagca
      301 cgtcgttctg gatggtgccg cgcagctcac tggggctgac gccctgctcc tcgccgacca
      361 gctggtacag cagcagcagc agcgacgctg gcgcgttgat cgtcatcgac gtggacacct
      421 ggtccagcgg gatgccgtcg aacaggatgc gcatgtccgc cagcgagtcg atggccacac
      481 cgaccttgcc gacctcgccc gccgcgatgt cggcgtcgga gtcgtagccc atctgggtcg
      541 gcaggtcgaa ggcgaccgac aggcccgtct ggccctgtga cagcaggtac ttgtagcggg
      601 cgttggactc cgcggcggtc gcgaagccgg cgtactggcg catcgtccac gggcgccccc
      661 ggtacatcgt cccgtacacg ccgcgcgtga acgggaactt gccgggctcg cccagcttgt
      721 cgccgtacgc gtcggccagg ccgggcgtgt ccgcgggcgt gtagacgccc ctggtgggga
      781 tgccggagac cgtcaggatg tccttgtcat ggctggccat gggcgcgatt gtaggcaccg
      841 gcgggaaccg tcggcacacg cgggagggcg gcgaatgggc cagacctcgg gattccaggt
      901 gcagggatcg gcaccggagc actacgaccg tcacgtggcg gtgttcatgg cgcccatggt
      961 ccgggcgctg gtggagcggt ccgggctgtc ggccggcggc gcactgctcg acgtggcctg
     1021 cggcacgggg ttcgtcgcgc gcgcggcggc ccccgtggtc ggccccgagg ggcggctggc
     1081 cggggcggac gtgaaccccg cgatgctcga gcatgctcgc gccgtgtgcg caggcgtggt
     1141 gcctccgatc tccttccacc acgcgcccgc ggagtcgatg cccttcggcg aggcggagtt
     1201 cgacgcggtg ctgtgccagc aaggcctgca gttcttcccc gacgtggacg ccgcgacggc
     1261 cgaggccgcc cgtgtcctgc gaccgggcgg caggtacgcc gccaccgtgt ggacggccct
     1321 ggacgattcg ccgtacttcg tggcgcagtg ccaggcggtc cgggccctgg cgggcgacga
     1381 cgacgcggac gccttcgcct ccgccttctc gttcccgccc gaccggctca ccggcgccct
     1441 caccaacgcg ggcctccgcg acgtcgaggt ggagacgatc gcgcccgtgg tcgagctgcc
     1501 gccgctggag gacttcgtgc cgggccactt cggcgcgatc ccgtggggag gcacgatcgc
     1561 cgagcagccg gacggcctgg ccacggccac ccggatgatc gcggaggcac tcggcgaccg
     1621 ggtgcgcgcc gacggctccg tggccgtccc cttcggcgtg ctggtggtgt ccgggacgcg
     1681 atgacgggag cgtccggctt ccaactgcgc gcgggtggcc cggcgtccta cgagcgctac
     1741 aacgccgtgt tcatggcgcc actcgtggcc gcactgctgg agcggtccgg gctgggcacc
     1801 ggcggcgcgc tgctggacct ggcctgcggt cccggcctca tcgcgcgagc ggcagcggcc
     1861 atcgtcggcc cgcaggggcg cctggacggg gtggacgtga acgggccgat gatcgcgcac
     1921 gcgcgcgcgg cgtcctccgg cacggtgccg tcgatcacct accacgaggc gccggccgag
     1981 gcgctgccgt tggccgacgc ggagttcgac gcggtcctgt gccagcaggg catgcagttc
     2041 ttcaccgacc tggacgcggc cgtcgcggag tgcgcccgcg tgctacgccc cggcgggcgg
     2101 ctggccgcca cggtgtggtc cgccctggag gagtcgccct acatgcaggc ccacagcctg
     2161 gccctggcgc ggctggaggg accggaagag ggagccgcgg tcgccgcgcc gttctcgttc
     2221 cccccggagc ggctgaccgc cgccttcgag caagcgggcc tgaggaacgt cgccgtggag
     2281 cgggtgtccc ccgaggtgga gctccctgcc ttggaggagt tcgctcccgg gcacttcgcc
     2341 ggggtgccgt gggggcggcg cacgtcggcc gacccggtgc agaccgccaa ggcggtggcc
     2401 ctcatgaagg agcacctgcg ccgctggacc cgaccggacg ggcgcgtcgt ggtgccgttc
     2461 tcctccgtgc tggtttccgg cgtgcgctga ggacgcggcg attggggggc gaaacggctg
     2521 cgggtgtagc ctgacgcgcg cagcccggtc atccgtcggg cgcgacggtc ccgctccaac
     2581 aaggacttcc gcatggcgac gagcaccgcg cacgcaccgg cgcgcaagag cttttaccgc
     2641 ggcggcgagg gcatgttcac ctgggtcctc caccgcgtca ccggtgtcct gatcttcgtc
     2701 ttcctgttcg cccacatcgt ggacacgagc ctgatcgtgg tgtcgccgca gctgtacgac
     2761 ggtgtgatcg aggtgtacca ccacccgctg atcaagctga tggagatcgg cctggtggtc
     2821 gccgtgctgt accacgcgtg caacggcatc cgggtcactc tggtggactt ctggtccaag
     2881 ggcgcgcagc gcaaccgcgg gatgatgcgc gtccagcgcg tcgtgttcgt ggcgctgtcg
     2941 gcgggcgccg ccatccccat gaccctccag ctcatcaacg acctgtaggc gggacgcgac
     3001 catgactcag ctcaacaccg ccgagaaccg ctccagggcc atgcagccgg cgcggcagcg
     3061 cacctcgtcc aacaccgagc tgtactcgtg gatcttcatg cgggtcagcg ggatcctgct
     3121 ggtgctcctg ctgttcgggc acctgtggat catgcacctc atcgagggcg gcgtgtcccg
     3181 cgtgaacttc gcgttcgtgg ccgggcgcct ggcgagcccg ttctggcaga cctacgactg
     3241 ggcgctgctg ttccttgcca ccatccacgg catgaacggc atgcggatca tcatcagcga
     3301 ctacgtgcgc acgccgcaga agcgcttctg ggcgaagatg gccctgtacg ccaccgccgg
     3361 cttcatgctg ctgctgggca cgctcgtcat cgtcacgttc gaccccgcgc tgccgctcga
     3421 aggctaggaa gacgtcaccc atgatccaca cccacaccta cgacgtcgtc atcgtcggcg
     3481 cgggcggcgc cgggctgcgc gccgccatcg aggccgccgg caaggtccgc acggccgtca
     3541 tcaccaagct ctaccccacc cgctcccaca ccggcgccgc gcagggcggc atgtgcgccg
     3601 cgctcggcaa cctggaggag gacaagtggg agtggcacgc gttcgacacg gtgaaggggt
     3661 cggactacct gggtgaccag gacgccatcg acgtcatgtg caaggaggcc gtggacgcgg
     3721 tcatcgagct ggagcacatg ggcctgccgt tcagccgcaa cgacgacggc accatcgccc
     3781 agcgcccgtt cggcggccac acccgcaacc acggcgaggc gccggtgcgc cgcgcctgtt
     3841 acgccgccga ccgcaccggc cacatgatcc tgcagacgct gtaccagcag tgcgtgaagc
     3901 gcgacgtgga gttcttcaac gagttctacg tgctggactt cctgaagtcc ccggacggca
     3961 cgcagaccgc cggcgtggtg gcctacgagc tgcgcaccgg cgacctgcac gtgttccggg
     4021 ccaagtccgt ggtgttcgcc tccggcggct tcggcaagat gttcaaggtc acctccaacg
     4081 cgcacgcact caccggcgac ggccccgccg tgctgtaccg gcgcggcttc ccgctggagg
     4141 acatggagtt cttccagttc caccccaccg gcatctaccg gctgggcatc ctgctgtcgg
     4201 aggcggcgcg cggcgagggc ggcatcctgc gcaactccga cggtgagcgc ttcatggagc
     4261 gctacgcgcc gaccatcaag gacctcgcgc cgcgcgacat ggtgtcccgc gcgatccgcc
     4321 aggagatcaa cgagggccgc ggcgtcgggc ccaacggcga ctacgtgctg ctggacctga
     4381 cgcacctgcc caaggagcag ctggacgaga agctgccgga catcaccgag ttcgcgcgca
     4441 cctacctggg cgtggagccc tacagcgagg gcgtgccgat ccagccgacg gcgcactacg
     4501 ccatgggcgg catcccgacc aacgtgaacg cgcaggtgcg caccggcgcg ggcgagacct
     4561 tcccgggcct gtacgccgcc ggcgagtgcg ccagcgtgtc ggtgcacggc gccaaccggc
     4621 tgggcaccaa ctcgctgctg gacatcgtgg tgttcggccg gcgcgccggc gtcgcggcgg
     4681 tcgagcacgc gctgtcgacg cgggacgtcg cggacctgcc ggagaacccg accgccgacg
     4741 tcgaggccca gctggagcgc atccgctcga acaccacggg cgagtccgcg gtgaagatcc
     4801 gcgaggagct ccagcagtcg atgaacgaca acgcccaggt gttccgcacc ggcgagctgc
     4861 tggccgagca gcagcgcatc ctgcagggct tgcagcagcg ctacgacggc atccgcatcg
     4921 acgacaaggg caagtcgttc aacaccgacc tggtcgaggc ggtggagctg ggccacctgc
     4981 tggacctggc cgaggcgctg gtcgacggcg cgatgacccg ggaggagtcg cgcggcgcgc
     5041 actaccgcga ggacttcccc aagcgcgacg acgccaactg gctgaagcac acgctggtgt
     5101 accgcaaccc ggacggcagc cggcggttcg actaccgccc ggtcacgatg ggccgctacg
     5161 agccgatgga gcgcaagtac tgatgttcct catgaagcga agcgttagga acctctagat
     5221 ggaagccacg ctgcgagtcc tccggtacaa ccccgaaggc gacaccgagc cgcacttcga
     5281 ggagtacaag gtccccgtgg acccgatgga ccgtgtcctg gacgcgctcc accacatcaa
     5341 gtggaacttc gacggctcgc tgtcgttccg ccgctcctgc gcgcacggca tctgcggctc
     5401 cgacgcgatg ctcatcaacg ggcgcaaccg cctggcgtgc aaggtcctca tgaaggacct
     5461 gcccggcacg gtcaccgtcg agccgatcaa gggcctgcgc atcctcaagg acctgatcgt
     5521 cgacatggac gcgttcttcg agtcctacaa gtcggtgctg ccgttcctga tcaacgacga
     5581 gccgaccggc gacaaggagc ggctgcagtc gcaggaggac cgcgagcgct tcgacgacac
     5641 caccaagtgc atcctgtgcg cctcctgcac cacgtcgtgc ccgatctact ggccggacaa
     5701 cagctacgtc ggtccggcct cgatcgtgaa cgcgcaccgg ttcatcttcg acagccgcga
     5761 ccgcggcgcg gccgagcgcc tggacaagct ggccggcgcc gacggcgtgt ggaagtgccg
     5821 cacgacgttc aactgcacga acgcctgccc gcgcgacatc gacgtcacca ccgcgatcca
     5881 gcaggtcaag cgcgagctga tcttcaacag agtctgatgg aacagcgcgt ctcgctgatc
     5941 acgctcggcg tcgccgacgt gcaccgggcg tggcgcttct acgaggcgct cggctggacc
     6001 ggcgagacac ccgacgacga cgtcgtgttc ttccaggcgg gcggcatggt cgtcgcgctg
     6061 tggagccgcg agaagctcgc cgaggacagc gtcgtgactg atcccggcgg ctggggcggc
     6121 gtgacgctgg cctactgcac ccgctcgcgc gaggaggtcg acgagatcct ccgcacggcc
     6181 gaggccgggg gcgcgcggat cggccggtgg ggcgcggaga cgttctgggg cggctactcg
     6241 gggatcttcg tcgaccccga cggccacccc tgggaggtcg cgcacaaccc cggctggatc
     6301 gtcaccgagg acggccggac actgctgcgc tgaccgacgg gcgcggcttc agctcattgc
     6361 ggcgatgatc gcgccggcga tggcgagccc ggcggcgtcg taggcgacgc tgatggcgac
     6421 cagcgtgcgg ttgcggctct cgaagaacgc gttcaccgcg tcggccgcgc catggatcag
     6481 cacgccgatc agcaggccga cgcccgccgc ctccgcgacg ccgagatcat cggcgatcca
     6541 gccgagcacg accgccaggc cgacggactt ggccagcccg ccgaggaacg cccacacgta
     6601 catcagcgga gaggagccct cccgcagctc ctcctcggtc ttgccgacgg cgcgcgtgaa
     6661 cggcttggcg aacagcagcg gggagtacca cagcccgccc aggacgaagt gggcgacggc
     6721 cgccaccagc accgcgagcg gatcgatctg caccggctcc atgaggacct cctgaccgct
     6781 cggccacccg cgggcgcgga cggcgccact tagagtgcgc gaccatgacc gaccacatca
     6841 tgcgggaact ggccgccgac gaggcgcatc tggcggcggc cgcgctgctg gcgctgcgcc
     6901 cgcacttcgc cggcgaggac gcgatcacgg cggcggtgcg gcgccagatg gcgcagggct
     6961 accgcctgct ggccgcgttc gcgccggggg acccggcggc cgcggccgtc gcgggtttcc
     7021 gcgtcgtgga gtacctggcg tgggggcgcg ccctgtacgt cgacgacctg agcacgcgcg
     7081 aggagcaccg gcgcgccggc cacgcccgcg cgctgctcga cgagctcatg gccctggcgg
     7141 aacgcgaggg ctgcgcccag ttccacctgg actcgggctt cggggcgtcg cgcgcgaacg
     7201 cccaccggct gtactacgag gtggggctgt accccacctc gctgcacttc tcctcgcggg
     7261 tgccgccgac gccgcgcggc tagagcgcgg ccttgcggta catcgtgacc acgacggcgc
     7321 cgcccagccc gatgttgtgc tggagcgcca cgcgcgcgcc gtcgacctgc cgggcgtcgg
     7381 cctgcccgcg cagctgccag ttgagctcgg cgcactgcgc caggcccgtg gcccccagcg
     7441 ggtggccctt ggagatcagg ccgcccgacg ggttgaccac cacgtcgccg ccgtaggtgg
     7501 tggcacccgc cagcgcgagc tcgccgccct tgccctcggg cgcgatgccc agcgcctcgt
     7561 aggtgatgat ctcgttggcc gagaagcagt cgtgcagctc gatgacgtcg aggtcctccg
     7621 ggccgaggcc ggactgctcg tagacctggc gcgccgcctc gcgcgtcatg tcggcgccga
     7681 ccagcgagat cacgctgttg gtcgagaacg tggattccat gtccgtgacc atcgactgac
     7741 ccaggatctc cacggccttg tcctccaggc cgtgctcgcg cacgaaccgc tcgctcgcga
     7801 gcaccgccgc accggagccg tccgaggtcg gcgagcactg cagcttggtc agcgggtcgt
     7861 ggatcatcgg gctgctgagg atctgctcca gcgagtagac gtcctggaac tgcgcgtagg
     7921 ggttgttggc cgagtggcga tggttcttct cccccaccgc ggcgaacatc tcggcggtgg
     7981 agccgtagcg ctccatgtgc tcgcggccgg cgttgccgaa gatctgcggc gcgggcgggg
     8041 actgcgccgg ctcgcgcagc tcgaacatct tcatgtagtg cttgtccagg ggcagggtgc
     8101 ggtcggtgaa cttcgcgccc agggagccct tctccatctt ctcgaagccc agcgccagca
     8161 cgcagtccgc caggccgccg cggatgtgct gcgccgccag ccagagagcc gtggagcccg
     8221 tggagcagtt gttgttgacg ttcaccaccg gcacgccggt caggccgagt tcgtagacgg
     8281 cgcgctggcc ggcggtcgag tcgccgtaga cgtagccgac catggcctgc tggaccagcc
     8341 tgtagtcgat gcccgcgtcg gcgagcgcct tggtgcccgc ctccttggcc atgtccgggt
     8401 agtcccactc cttcgcgccc ggcttctcga acttggtcat gccgacgccg acgacgaaca
     8461 ccttctccac ggccggattc ctttcgactg gtctcgcgct cacgttgccg ccgattgtga
     8521 cacggttgga cgcggagcgt tccggtgacg cgggtgccgg agccgatagg gtgcggcgcg
     8581 agcgggaaag gagccaccca tggacgagcc acgcgacgac atgacgccct tcgaggcggt
     8641 gaactggttc ttccaccgcg ccgccgaccg tgtgggcctg gacgaccaga cccgcgccgt
     8701 cctggccggc acctaccgcg agatccgcgt ccaggtggcg ctgcgccgcg acgacggctc
     8761 cgtgcagacg gtgtacggct accgcgtcca gcacaacggc gcgcgcgggc cgtacaaggg
     8821 cggcgtgcgc ttccaccccg aggcggacct ggaggaggtg cgcgcgctcg cctccctgat
     8881 gacgtggaag accgccgtgg tggagctgcc gttcggcggc gccaagggcg gcgtgcaggt
     8941 ggaccccaag cagctgtcgc tgcccgagct ggagcgcctc acgcgccgct acatgtcgca
     9001 ggtgagctac gtggtcggcg tcaaccgcga catcatggcg cccgacgtca acaccaacgc
     9061 ccagaccatg gcctggatga tggacgccta cgggcagcgc aagggccaca cgcccggcat
     9121 cgtcaccggc aagccggtcg agctcggcgg ctcgctcggt cgcgaggcgg ccaccggccg
     9181 gggcgtcgtc atggtgacca acgagatggt ggccgccgca ggcaagtcac ccgagcagat
     9241 gaccatggcg atccagggct tcggcaacgt cggctcgtgg accgcccgcg tcggcgcggc
     9301 ggacggctac aaggtcgtgg cggtgtccga cgtgaacggg ggcatctacg ccgcgcacgg
     9361 cctgccggtg gcggacctgc tccagcacat cgaggagacc ggaaccgtgg tgggctaccc
     9421 gggctgtgac gcgctcacga acgaggagct gctggagctc gacgtcgacg tgctggtgcc
     9481 ggcggcgctg ggcggcgtga tcaccaaggc caacgccgac gcgatcaagg cgcggtgggt
     9541 cgtggagggc gccaaccacc cggtcacccc ggccgcggac ggcgtcctgg ccggcaacgg
     9601 catcacggtc gtccccgaca tcctggccaa cgccggcggt gtcaccgtgt cctacttcga
     9661 gtggacgcag aacatccagc agttcgcctg ggacgaggag cgcgtgaaca ccgagctcgg
     9721 caaggtcatg cgcgaggcgg tgcgtcaggt gcgggaccgt tccgtcgcgg acggcacgtc
     9781 actgcgcgac gcggcgttct cgatcgcggt ggagcgcgtc gcccaggccg cccacctgcg
     9841 cggctacgtc tgaggcgcct cgggcttgcg gaagatgtgc tcgcgatacc agcgcagctc
     9901 ggcgatggac tcgcggatgt cggccagcgc gcggtggccg ccgttcttct ctggacgctg
     9961 cgccaacgcc ccggggtacc agcgccccac cagctccttc agcgtcgaca cgtcgatgtt
    10021 gcggtagtgc aggtgcgctt ccagctccgg catgtgcttg cgcaggaaca tgcggtccgt
    10081 gccgatggag ttgcccgcca gcggcgccgt gcgcgcctcg gggacgtgct gcctgatgaa
    10141 cgccagggcc tcccgctggg cctcgcgcac gtcgatcttc gagtcgcgca ccgccttggt
    10201 gagcccggac acgccgtgca tctccaccac caccggcggc atgcgctgga gttcctcgtc
    10261 ggtggcgtac agcaccaggt cgggtccctc gatcacggtc tccagggagc cgtcggtgac
    10321 gatgacggcg atctccagga ccacgtcggt ctccgggtcc aagccggtca tctccaggtc
    10381 cacccacacg atgggatctt tcagcgcctc ggtcacgtcc tgctcctcag tcacaactcg
    10441 ctcacctgga gtcccagagc tcgcgccgca gcgcacaacg cacgatcgcc ggaaacgaac
    10501 agcacatcgt cgtccgccag ccgctcggcc gtggccaagt gcatcgcgtc gtatccccgc
    10561 aaggcttggg cgtcggccag ctcgcccgcg cggcggatca gctcctcatc gatgcgtgcc
    10621 tgggcgacat tccggtgcag cagctcgaac agcgcgaggg tggactggta ggaatatccc
    10681 gccagccggc tcaaccgacg tgccctgccc agcgcggcac gcgtttccac gtacagcagt
    10741 ggcacggaca ccacccggtc ggcatcgctc cacaagtcga gcgccgccgc ggacgcgggt
    10801 tcgtcgatga gcagcggcac aaacgcggag ctgtcgaagt acgcgatcac cagcgccgct
    10861 cttcgatgat cagatcgctc accgtcccgt cgatcttgat tggcttcggg acggtgcggg
    10921 gcccgcggtt cttcgcgggc gttaccagac cctccgcgat gaggcgatcg atcttgcgct
    10981 ccccggagag cggcaccagt cgcgctactg cgcgaccgcg gtcggtgacc acgatctcct
    11041 cgccctgccg cacctcgtcg aggtagcggc tgagatggtt gcgcaactcc cggatgccga
    11101 cttccatgcg ctggcccttc gatggcgcca cattgtgcat caccatggta gccacttccg
    11161 cctcctactc ctaggtcccg tcgtcggcga actcgctcaa ctccagccag cgtgtctccg
    11221 ccgcgtccag ctccgccagc agcgtcgcca gctcgtcgcc ggcttcggcg gccgcctcgt
    11281 agtccgcgcc ggcctcctgc acggcccgct ccagctgcgc gcgccgcgcc tccagctgcg
    11341 ggatgcgttc gctgagcagc gcgagctcgc gggtctcgtt gtagcccagc ttgcgccggc
    11401 ggcgctcccc ccgaggctgt tcgcctgtgg acaacggctg cgacgcggcg gcggccgccc
    11461 gttcctgctc ctgctcctgg gcggcctggt aggcggacca gccgccgggg tggtggcgca
    11521 gtgagccgtc cggctgcacc gaccagatgt cgtcgcacac gcggtccagg aagtagcggt
    11581 cgtgggaggc gaccacgagc gcgccctgcc agccgtccag gtactcctcc agcacccgca
    11641 gggtgtccag gtccaggtcg ttggtcggct cgtccagcag cagcaggttc ggcgcggtcg
    11701 ccagcacgcg cagcagctcc aggcggcggc gctcgccacc ggacagctcc tcgacgtagg
    11761 ccttctgcgc ctcgccggtg aacaggaagc gctccagcag ctgcgacgcg gacagcgtga
    11821 tgccctcgac cgtgttcgtc tccagcacca cctccttcag cgcgtcgatc acgcgcgtgt
    11881 tgggcatcgg cgtcggctcc tggcggtaga agccgggcac gatcgtctcg cccaccacga
    11941 ccttgccctc gtccggctcc agctcgccga cgatgagccg cagcagcgtg gacttcccgc
    12001 tgccgttggg ccccaccagg ccgatgcgcg cgtccggcgc cagcttgtgc tccacgccgc
    12061 gcaggaccca gcgctcgccc gcgccgtcgg cgaagcgctt gccgacgttg tggaggttga
    12121 ccaccttgct accgatgcgg cgcgatggca ggccgatctc cacgtcgcgg cgccgctcct
    12181 ccgcgcggaa ctccaccagg gccttggcgc gctccacccg gtacttggcc ttggacgtgc
    12241 gcgccttcgg cccgcggcgc agccactcca gctcagtgcg cgtgcggttg gcgcgcttgc
    12301 gctcctccgc ggcggcctgc tcctcccgca ccgcgcgcgt ctggaggtag tcggcgtagt
    12361 tgccgtagcc ggtgtggatg ccgccgcgct ccacctccac gatgcgcgtc gcgacgtcgt
    12421 cgagcaggta gcggtcgtgc gtcaccagga tgagggcgcc ctgccagccg agcaggcggc
    12481 cctccagcca ctcgatcacg tcgacgtcga ggtggttggt cggctcgtcc aggatgagca
    12541 cgttggcctc gtccgccagc gcgcgcgcca gcgcgacgcg cttgcgctcg ccgccgctgc
    12601 gctcgctcac ggggcggtcc cagtcccgca cgcccagccg gtccagcatc tcctccgcgc
    12661 ggtgctgcgg agcctcgatg ccgcgcagca cggcctgcag cggcgtgtcc gccgggtcca
    12721 ggccgggctc ctgcggcaag tagtggacgc gcgcgtcgtt ggccagcacg acgcgcccgt
    12781 cgtcgggctc ctccgcgccg gcgatgagcc gcagcagcgt ggacttgccg ctgccgttca
    12841 cgccgatcac acccacgcgg tcgccgtcgt cgatgccgag cgtgacctcg ctcagcacct
    12901 gtttctcggc gtggcgcttg ctcacccgct ccaggctgac gaggttcacc ggcgcgccat
    12961 ccgccggatc acgcggacag gccgacgcgc tcccgcacct gcgccatggt cgcggcagcc
    13021 acctcgcgcg ccttgtcggc gcccttgcgc agcgcctcct cgaccgcgcc gcggtcgtcc
    13081 ttcagcgact ggtagcgctc gcgcaccggg cgcaggaact cgttgacggc ctcggccaga
    13141 tccttcttga agtcgccgta gcccctgccg tcgtagtcgc gctcgagctg cgcgacggtg
    13201 cggccggtca cggcggccat catgtccagc aggttggcga tcccctgctt gtccggcagc
    13261 ggctccgccc tcacctcgct gcccgagtcg gtcaccgcgc gcatgacctt cttgcgcgtc
    13321 gcggtgtcct cctcggccag caggatggtg ccctgcggcg agtcggccga cttcgacatc
    13381 ttcttgtcgg ggtgctgcag gtccatgatg cgcgcgccgg cctccgggaa caccgccttg
    13441 ggcaaggtga acgtgtcgcc gaagcgggag ttgaagcgct gcgcgatgtc gcgcgcgagc
    13501 tcgatgtgct ggcgctggtc cgcgccgacc ggcacctcct gcgtgccgta cagcaggatg
    13561 tccgcggcct ggaggaccgg atagtcgaac agcccaacgc tgacgaagtc cttgccctcg
    13621 gccttgtcct tgaactgcgt catgcggccc agttcgccca tgccggccac gcagttgagg
    13681 atccacgcca gctcggtgtg ctcgcgcacc tggctctgca cgaagacggt gcagacctcc
    13741 gggtccaggc cggcggccag ccaccaggtc gccaggtcca cggtgcgggc gcgcagctcc
    13801 gccggcggga tcgggatggt gatggcgtgc aggtccacga cgcagaagta ctggccgggc
    13861 gcctggtcgc gcacccagcg gctgacggcg ccgaggtagt tgccgagatg cacctcgccg
    13921 gtcggctgca tgccgctgaa cacgatcgcc actgcccgtc accctccttg tcgtcgaggg
    13981 catcgtagcg gcgccggggc gccccccttc ccgggatggg tgcatcccgg gcgccatctg
    14041 gcccggagtg cgcccacagg ggcgcggatt ctgtggatca gtcctctcgg gtggtggcgc
    14101 cgcgggcggc gatctccgag acgatgtcgg cgttggccag cgtggtgacg tcgccgagct
    14161 cgcgcccgtc ggcgatgtcg cgcagcaggc gccgcatgat cttgccggag cgcgtcttcg
    14221 gcagatcgtc ggtgaacagg atgttggccg gccgcgcgat cttgccgatc tcggtgccga
    14281 cgtgctcgcg cagctccgcg gccagctcgt cgcctggctc gttgccgccc ttgagcgtga
    14341 cgtaggcgac gatcgcctgg cccgtcatgg cgtcctgacg tccgacgacc gcagcctcgg
    14401 cgaccttcgg gtggcccacc agcgcgctct cgacctccgt ggtggagatg cggtgcccgc
    14461 tgacgttcat cacgtcgtcg acgcggccga gcagccagat gtgcccgtcg tcgtccagct
    14521 tcgcgccgtc accggcgaaa tagacctcgg tgccgaagcg ggaccagtac gtgtcgacgt
    14581 agcgctggtc gtcgccccac agcgtgcgca gcatgcccgg ccacggacgc tgaagggtca
    14641 ggtagccgcc gccgcccggt ggcaccgggt tgccctcctg gtccacgacc acggccttca
    14701 cgccggggaa cggcgtcatc gcgctgccgg gcttggcggc ggtgacgccg ggcagcggcg
    14761 tgatcatgat cccgccggtc tcggtctgcc accaggtgtc gaccaccggg cagcggccgc
    14821 cgccgatggt ggtgtggtac cagacccatg cctcggggtt gatcggctcg ccgacggagc
    14881 cgagcaggcg caggctggac aggtcgtgcc tggccggcag ctccgtgccc cacttcatga
    14941 acgtgcggat cgcggtgggt gccgtgtaga ggatcgtggc cttgtagcgg gcgatcatct
    15001 cccaccagcg gtccttgtcg gggaagtccg gcgcgccctc gtagaggatc gacgtggcgc
    15061 ggttcgcgag cggcccgtag acgatgtagc tgtgcccggt gacccagccg atgtccgcgg
    15121 cgcaccagta gatgtcggtg tccggcttga ggtcgaacac gagctggtgg gtggcggcca
    15181 cgtgggtcag gtagccgccg gtggtgtgct ggatgccctt cggcttcccg gtgctgccgg
    15241 acgtgtagag gatgaacagc gtgtcctcgg cgtccatcgg ttccggcggg cactccgtgg
    15301 gctggccggc gacggcctcg tgccaccaca cgtcgcggcc cgtggtgaag ccgacgtcct
    15361 cgcccgtgcg ccgcaccacc acgcagcgct cgatcaggtc ggtctgctcc atggccgcgt
    15421 cggcggcgtc cttgagcttc accacggcgc ccttgcggta gctgccgtcg gcggtgatga
    15481 gaaagcgcgc cttggcgtcc tcgatgcggt cgaccagggc ctgggagctg aagccggcga
    15541 acacgacgct gtgcggggcg ccgatgcgcg cgcacgccag catcgcgacg ggcagctcgg
    15601 ggatcatcgg caggtagatg gtgaccgggt cgcccttctt gacccccatc gacttgagcg
    15661 cgttggcgaa gcggctgacc tcgtccttga gctccgcgta cgtgatcgcg cgctgctccc
    15721 caggctcgcc ctcccagtga tacgcgacct ggccgccgtg gccggcctcg acgtggcggt
    15781 ccaggcagtt gtcggagacg ttgagcttgc cgcccaggaa ccacttggcg tgtggcggct
    15841 tccagtcaag gacggtgtgc cagggctcca tccagcgcag gcgttcggcc tggctgaccc
    15901 accagccctc ggggtcgcgc tcagcctgct cgtagatgga ggcgtcactg acgttcgcct
    15961 gcgccgtgaa ctccggtgag ggcgggaagg tccgcccctc gcgcatcagg ttctcgatcg
    16021 ccgcgtccgt catggcgcac cactccttgt gtcgcttcct cgcccgcgca gcctcgcgcg
    16081 acgagcgcag ccaggcaagg gcgcgaccgc cggccatgcg gcaccaggcg agcaaaaccg
    16141 gggtgtgtcg actcaatcaa tcggagagtg actcagtcga cacaccctgg gtggaacgtc
    16201 ccggagcagg gctagtagtc gtcctcggct tcgagggtga ggccctcgag ctcgtcgcgg
    16261 acggtgtcgc ggtcgtggtc gctggcctcc agggcgtcgc tgcgctcgcg cgcgatgcgg
    16321 cgcagatcat ccggatcgat cccgtcgtcg gccagggcgc gcagctcctc gtcggacagc
    16381 tcgacctccg ggcgcgccat cacgcggctg cgcacgcgct ggcccgcggt ctgggcgccc
    16441 tccgggcgcc ccgggcggtt gcgatgtggc atgaggctcc ccctcgggct tcggcggctg
    16501 gcgtctgcgg ctgcccgcta caccagcgag tcgacgtcgg actgcttctc ccggaccgcc
    16561 tcggccgcgg tcttcaaggc gtccagctcc tgcggctcca ggtccagctc gacgatttcc
    16621 tcgacgccgc cccgtccaat cctggccggc acgccgaggt acacgtcgct gatcccgtat
    16681 tccccggtga ccagggcgca caccggcatg acgtccttgg tgtcctcggc gatcgcgcga
    16741 accatcttgg ccgcggccga cgacggcgcg tagtacgccg agccggtctt cagcagcgcc
    16801 acgacctccg cgccgccgtc acgcgtggcc tgcacgatct gctcgatctc gtccgccgac
    16861 agcaggtcgc gcagcggctt gccgtccacg gtgaccttcg acggcaccgg caccatggtc
    16921 tcgccgtggg agcccagcgt gatcgcctcc acggccagcg gcgacacgcc cagcttctcg
    16981 gcggtcttgt cggcgaagcg ggcgctgtcg agcatgccgg cctggcccat aacgcgcgcc
    17041 gaagggaagc cggacacctt ctggaacagc gcggtcatct cgtccagcgg gttggacacc
    17101 acgatcacca cggcgtccgg ggacgtgcgc gcgacctcct tcgccacgcc gccgacgatc
    17161 tgggcgttgt ccttcagcag gtccatgcgg ctcatgccgg gcttgcgcgg cttgccggcc
    17221 gtcaccacca cgatgtcgct gcccgccgtc gcggcgtagt cgttggtgcc gatcaccttg
    17281 gtgcggaaac gctcgatcgg gcgcgactgg ttcatgtcca gcgccaagcc ctggggcagc
    17341 ccctcgacga tgtcggtcat gacgacctcg tccgcatagc cggcctcggc gatgcgctgc
    17401 acggtggtcg agccgtactt cccggcgccg acaacggtga tcttcacggg gcactccttg
    17461 gagacgatgc gggacaggct tgtcgcggcg gagcctaccg c
//
LOCUS       DATMKC010000092         9143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_136025, whole genome shotgun sequence.
ACCESSION   DATMKC010000092 DATMKC010000000
VERSION     DATMKC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136025"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VNU01_02655"
     CDS             complement(<1..1038)
                     /locus_tag="VNU01_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWH31553.1"
                     /translation="MQRICAVALVGALSLLATACGTEAGNEPAEGTTVSSGDGTVDAV
                     GFIYIGPKDDFGYNQAAYEGSLAVEEAFGDVTVLQAENVPETTEAEVVMEDMISQGAD
                     LIFATSYGHLEFAANLAARHPDVVFVHQGGLEGDSKLDNLGTYFGTVYEPVYTAGIAA
                     GKASRTGKLGYVYAFPIPQTLANINAFTLGAQSVNPDIETITISTGNWCDPGLQAEAA
                     KSLIDQGADVITQHQDCTKTIIEATEAAGLYSVGYHADASELAPEGWLTGSEWDWETL
                     YVDIVETVMNGEFADSPYNGDFRVGLQTGDNPFVQSAFGPAVDEETQALIDDARQRFI
                     DGGSPFAGPVVD"
     gene            complement(1079..1981)
                     /locus_tag="VNU01_02660"
     CDS             complement(1079..1981)
                     /locus_tag="VNU01_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH31554.1"
                     /translation="MLLEAVLTGAVRGGTSILYAALGEVVAERSGVINLGTEGCMLSG
                     ALAAYIVGVRTGNPWAGAAAGLAAGALLALVFAGVVIGRRANQLAVGLVVSFLGLGLT
                     SLFGQAYVGQGVEAFSRVPVPLLADLPLVGTVLFQHDPLTYLSFVAAPAVWWMLFRTK
                     TGLRVRAAGERPEVLDAFGISAARVRCAAVVAGGALAGLGGAQLSTAFALNWSENMTV
                     GRGFVAVALVIFAAWHPLRAVGGAYLFAGALALQLQLQARGVDVNRYLLESLPYLTVL
                     VILAVMSRHRLHHGPEALAKVFDG"
     gene            complement(1981..3114)
                     /locus_tag="VNU01_02665"
     CDS             complement(1981..3114)
                     /locus_tag="VNU01_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH31555.1"
                     /translation="MSQVAPPAARDAEPPEGHDTTWRDGLVRAPESARRRLAAAASAA
                     LPGAARWTAVIGAGVVVYAVFLALQGASPFAVLRAMAVSAAGSQTAIAETLVRATPLL
                     LAALAVAVPARAGLFNIGGEGQLLMGAVSAMGAARLLDGALPPPLSLIALALAGVAGG
                     ALWALVVGVLRVFSSTSEAISSLLLNYVAGLVLTWLVFGPWKDPGSLGQAYSRSLEGP
                     ERLPILWGHRFHLGVLIALAVAVGVWLLLRQTSWGFQLRIVGGNPEAARRAGLRVGEL
                     HLGALLLGGALAGLGGMVELAGVEARLRPDMLFGFGFVGFLASWLARHHALKAVAAAV
                     VLGAIAVGGNGLKLASGLSGSAVNILMAVLLLAVLGWAPGRSR"
     gene            complement(3111..4622)
                     /locus_tag="VNU01_02670"
     CDS             complement(3111..4622)
                     /locus_tag="VNU01_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWH31556.1"
                     /translation="MTAPPAVLQAVDVTVRFGDVVANRAVNLDIQRGEVHALLGENGA
                     GKSTLMKVLYGVNRPQEGAILVDGREVRLDAPSDARALGIGMVFQDLRLVPALTVEEN
                     IALSVSSLPRGRDARRQVVLAAGVHAGIVVQPERLVRDLSLAERQQVEILRALMAEAR
                     ILILDEPTSALAPQEVESLLATITALRERGLAIVLITHKLAEARGVADRVTVLRGGRV
                     VLAGADPRTLSDDELVAHMVGVTVPPLPSDRVGTRSHEHALVVEDLDADTDDGRPALR
                     QVALRVGRGEIVGVAGVSGNGQRELIDAVMGLCRHASGRVTVNGRRIRHGVPADALEA
                     GAVAIFEDPQAASVVPGLDVCEHLVLDGRPFPRSQLTLDWRMLRRRAGELRDAAPLRL
                     APLQAQVSTLSGGNVQRVLLGRAFAKQPLTLLVLAYPARGLDIASVRATQQVLLTQRA
                     AGVGVLMVSEDLDELLSVADRIVVLHGGAVAGTVLPTRTTRQEIGRLMMRGAA"
     gene            complement(4619..5341)
                     /locus_tag="VNU01_02675"
     CDS             complement(4619..5341)
                     /locus_tag="VNU01_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWH31557.1"
                     /translation="MQDDTDGATRTEALTGRIRRDIATNQLPAGMRLTEKGLARRYGV
                     SRTPVREALRSLAQEALLEYAPHTGYRVTRLLLSDLDDLYAVRIAVETHAVRRLAGGV
                     GDRETVEDLLATWSRSPQEAPPPADADGTLISADEDFHEALASAAGGSVLLETLRTIN
                     RRIHAIRMREFIDAERVSRTFEQHAGIARAILAGDPDLAVALMHAHILEGHRYVRAYA
                     LAHGLVEHAGLDLPGLREEYAP"
     gene            complement(5490..5882)
                     /locus_tag="VNU01_02680"
     CDS             complement(5490..5882)
                     /locus_tag="VNU01_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWH31558.1"
                     /translation="MVTRVLDAWAVVAYLHGAEPAASRVAGLLRRGRPVMSWVNLGEV
                     HYVVARRHGAREADQVVRDLRHRLTLEAPSPGRVLDAARLKASYRLSYADAFAAATAV
                     AADGLLLTGDPELLADGAPWRAEDLRRS"
     gene            complement(5876..6106)
                     /locus_tag="VNU01_02685"
     CDS             complement(5876..6106)
                     /locus_tag="VNU01_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWH31559.1"
                     /translation="MTHRVGPKGQVVIPKELREELGIAPGDEVTFWREGAHVALAPVR
                     SRRPLKGRFAGSDLLGVLAAERAEERTRDQRW"
     gene            complement(6173..7003)
                     /gene="fdhD"
                     /locus_tag="VNU01_02690"
     CDS             complement(6173..7003)
                     /gene="fdhD"
                     /locus_tag="VNU01_02690"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWH31560.1"
                     /translation="MSARKRIEKTPVLKLRDGEATRAWDALAAEEPMEIRVSGEAVSV
                     TMRTPGHDFELALGFCLTEGIVADAHAVETMRYCAGEDPETGLNTYNVVDLHLRGGVT
                     VAPELKRNVYTTSSCGICGTASLEAVRRRATPVHDDPLRVPAALLDGLPGKLRAAQEV
                     FDRTGGLHAAGLFDAGGTLLCLREDVGRHNAVDKVIGWAATEGRLPLRSHMLMVSGRV
                     AFEIAQKALLAGIPLVAAVSAPSSLAVDLARETGMTLIGFLREGRMNVYAGEERVENV
                     "
     gene            complement(7000..7242)
                     /locus_tag="VNU01_02695"
     CDS             complement(7000..7242)
                     /locus_tag="VNU01_02695"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HWH31561.1"
                     /translation="MAEDSVEPFFRDLAATAGTVPLSDAEADAVLDLTRVVAHTRERR
                     LAPLTAYAAALAVAELDEAGRIAHLRALIEQLRDSA"
     gene            complement(7277..7918)
                     /locus_tag="VNU01_02700"
     CDS             complement(7277..7918)
                     /locus_tag="VNU01_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWH31562.1"
                     /translation="MTGTPYIAAVLLDLDGTLVDSVYLHVQTWQQALREAGLDVPAWR
                     VHAGIGMGSDRLVPWLLGRHTEQQSELSDAHHRLFLERADELRPTRGARELVEDLERR
                     KVPFTIATSAKPPVRKALLAALGRQDLDSTDAGDVDSPKPAPDLLLAACQELGVDPDR
                     ATLVGDSPWDAEAAIKVGMRCLAVRTGGFGDDRLLDAGADDVVDDPLALVGRL"
     gene            7965..>9143
                     /locus_tag="VNU01_02705"
     CDS             7965..>9143
                     /locus_tag="VNU01_02705"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HWH31563.1"
                     /translation="MTELDDVRARAERWLAHDPDPDTVAELRALLADAEPTLLTARFG
                     ERLPFGTAGIRGAIGVGPAAMNRLVVRQVSAGLARRLLAEPDASSRGVVVGQDARHKS
                     DEFAADAAAVFAAHGLPVHRFDGPVPTPLVAFAVRMLGAAAGVQVTASHNPATDNGYK
                     VYWGDGGQITAPLDTEIAAEIDGIAEPWDVALAEPGDSLLGEVPPGVGESYLKLTLDQ
                     RVAPGATALRIVYTPLHGVAWATVRELLSRAGYGDVDVVAEQAEPDPDFPTVGFPNPE
                     EPGALHLAMALAARRGADLLLANDPDGDRIAAAVPEPDGRWRALSGDEIGCLLADHVL
                     TYGGGGDERVVATTVVSSRLLSRIAADHDVGYAETLTGFKWLAAEAAAAEARGGRMVL
                     A"
BASE COUNT         1263 a         3306 c         3379 g         1195 t
ORIGIN      
        1 gtcgacgacc ggtccggcga acggcgaccc gccgtcgatg aagcgctgcc gcgcgtcgtc
       61 gatcagggcc tgggtctcct cgtccacggc ggggccgaac gcggactgca cgaacgggtt
      121 gtcgccggtc tgcagcccga cgcggaagtc gccgttgtag gggctgtcgg cgaactcgcc
      181 gttcatgacc gtctcgacga tgtcgacgta cagcgtctcc cagtcccact cggatccggt
      241 gagccagcct tccggcgcga gctcggaggc gtcggcgtga tagccgacgg agtacagccc
      301 ggccgcctcg gtcgcctcga tgatggtctt ggtgcagtcc tggtgctgcg tgatgacgtc
      361 ggcaccctgg tcgatcaggc tcttcgccgc ttccgcctgg aggcccgggt cgcaccagtt
      421 cccggtggag atggtgatgg tctcgatgtc cgggttcacg gactgggcgc cgagtgtgaa
      481 ggcgttgatg ttggccaggg tctgggggat ggggaaggcg tagacgtacc cgagcttgcc
      541 ggtccggctg gctttgccgg cggcgatgcc ggcggtgtag acgggctcgt agaccgttcc
      601 gaagtaggtg ccgaggttgt ccagcttgct gtcgccctcg aggccgccct ggtgcacgaa
      661 gacgacgtcc gggtggcggg cggccaggtt cgccgcgaac tccaggtgcc cgtagctcgt
      721 ggcgaagatg agatcggcgc cctggctgat catgtcctcc atgaccacct cggcctcggt
      781 ggtctccggc acgttctccg cctggagcac ggtgacgtcc ccgaatgcct cctcgaccgc
      841 caggctgccc tcgtaggcgg cctggttgta gccgaagtcg tccttggggc cgatgtagat
      901 gaaaccgacg gcgtcgacgg ttccgtcgcc cgaggacacc gttgtgccct cggctggttc
      961 gttgcccgct tcggtaccac aggcggtcgc cagcagcgac agcgccccga ccagggctac
     1021 cgcacagatg cgttgcattg tctcgctcct tgggttcggt ggaacaggcg gacggcgttc
     1081 agccgtcgaa gacctttgcg agggcctcgg gcccgtggtg gaggcggtgg cggctcatca
     1141 ccgcgaggat cacgagcacg gtcaggtagg gcaggctctc cagcaggtag cggttgacgt
     1201 ccacgccgcg tgcctggagc tggagctgga gggcgagggc tcccgcgaac aggtaggcgc
     1261 cgcccaccgc gcgcaggggg tgccaggccg cgaagatcac cagcgcgacc gcgacgaagc
     1321 cgcggccgac ggtcatgttc tcggaccagt tgagcgcgaa ggcggtggag agctgggcgc
     1381 cgcccaggcc cgcgagcgcg ccgccggcca cgaccgccgc acagcgcacg cgcgcggccg
     1441 agatgccgaa ggcgtccagc acctcggggc gttcacccgc cgctcgcacc cgcagcccgg
     1501 tcttggtgcg gaagagcatc caccacaccg ccggcgccgc gacgaacgac aggtacgtga
     1561 gcgggtcgtg ctggaacagg accgttccca ccagcggcag gtccgccagc agcggcaccg
     1621 gcacgcgcga gaacgcctcg acgccctggc cgacgtaggc ctgaccgaag aggctggtca
     1681 ggcccagccc caggaagctc acgaccagtc cgacggccag ctggttggcg cggcgtccga
     1741 tcaccacccc ggcgaacacc agcgccagca gggcgcccgc ggccagtccc gccgccgcgc
     1801 cggcccacgg attgcccgtg cgcacgccca cgatgtaggc ggcgagcgct ccgctgagca
     1861 tgcagccctc ggtgcccagg ttgatcaccc cgctgcgctc ggcgaccacc tcgccgagcg
     1921 ccgcatacag gatcgacgtg ccgcctcgca cggcgccggt gagcacggcc tcgagcagca
     1981 tcagcgggac ctccccggcg cccagcccag gacggcgagc aggagcaccg ccatcaggat
     2041 gttcaccgcc gagccggaca ggccggacgc cagcttgaga ccgtttccgc ctaccgcgat
     2101 ggcgccgagc acgacggctg cggcgactgc cttcagcgcg tggtggcgag ccagccagga
     2161 tgcgaggaag ccgacgaacc cgaagccgaa gagcatgtcg ggacgcaggc gagcctccac
     2221 tccggcgagc tcgaccatgc cgcccagccc ggccagcgcg ccgccgagca gcagggcgcc
     2281 aaggtgcagc tcgcccacgc gcagtcccgc gcggcgcgcg gcctccgggt tgccacccac
     2341 gatgcgcagc tggaagcccc aggacgtctg gcggagcagc aaccacacgc cgaccgcgac
     2401 ggcgagggcg atcagcacgc cgaggtggaa ccggtgtccc cacaggatcg gcagccgttc
     2461 gggtccttca agcgagcgcg agtacgcctg cccgaggctg cccgggtcct tccaggggcc
     2521 gaacacgagc caggtgagca ccaggcccgc gacgtagttg agcagcagcg aggagatcgc
     2581 ctcgctggtc gagctgaaca cgcgcagcac accgacgacc agcgcccaca gggcaccgcc
     2641 ggcgactccg gccagcgcga gggcgatgag gctgagcggg ggtggcagcg cgccgtcgag
     2701 caggcgcgcg gcgcccatgg cggacacggc gcccatcagg agctgtccct cgccgccgat
     2761 gttgaacagg cccgcgcgcg ccggcaccgc cacggcgagc gcggccagca gcaggggcgt
     2821 cgcgcggacc agggtctccg cgatcgcggt ctggctgccg gcggcggaga ccgccatggc
     2881 acgcagcacc gcgaacggcg acgcgccctg gagggccagg aaaaccgcgt acaccaccac
     2941 cccggcaccg atgaccgccg tccagcgcgc ggcgcccggc agggcggcgg aagctgcggc
     3001 cgccaaccgc cggcgcgcgg actccggcgc gcggaccagc ccgtcgcgcc atgtcgtgtc
     3061 gtgcccctcg gggggctcgg cgtcccgcgc cgcgggtggg gcgacctggc tcatgcggcg
     3121 ccacgcatca tcaggcgccc gatctcctgg cgggtggtcc gggtcggcag gaccgtgccc
     3181 gccacagcgc cgccgtgcag gacgacgatg cgatcggcca ccgacagcag ctcgtcgagg
     3241 tcctccgaca ccatgagcac cccgacgccg gccgcccgct gcgtcagcag cacctgctgc
     3301 gtggcgcgga cggaggcgat gtcgagcccg cgcgccgggt aggccagcac cagcagcgtg
     3361 agcggctgct tggcgaacgc gcggcccagc agcacgcgct ggacgttgcc acccgacagc
     3421 gtcgagacct gcgcctgcag cggggccagc cgcagcggcg cggcgtcacg cagctcgcct
     3481 gcccgccggc gcagcatgcg ccagtcgagc gtcagctggc ttcggggaaa cgggcgcccg
     3541 tccaggacca ggtgctcgca gacgtccagc cccggaacga cggacgccgc ctgggggtct
     3601 tcgaagatcg cgacggcgcc ggcctccagc gcgtcggcgg ggacgccgtg ccggatccgg
     3661 cggccgttga cggtcacgcg gcccgacgcg tggcggcaca ggcccatcac cgcgtcgatg
     3721 agctcccgct gtccgttgcc ggacacgccc gcgacaccca cgatctctcc gcgcccgact
     3781 ctgagggcga cctgccgcag cgccggccgc ccgtcgtccg tgtccgcgtc gaggtcctcc
     3841 acgacgagtg cgtgctcgtg agagcgggtg ccgacacgat ccgagggcag cgggggcacg
     3901 gtgacgccca ccatgtgggc cacgagctcg tcgtcgctga gcgtgcgcgg gtccgcgccg
     3961 gccagcacga cccggccgcc gcgcaggacg gtgacgcggt ccgcaacgcc ccgtgcctcc
     4021 gcgagcttgt gggtgatgag cacgatcgcg agcccgcgct cacgcagcgc ggtgatggtc
     4081 gcgagcagcg actccacctc ctgcggcgcg agcgcgctgg tgggctcgtc caggatgagg
     4141 atgcgcgcct ccgccatgag cgcgcgcagg atctccacct gctggcgctc cgccagcgac
     4201 aggtcgcgca ccagtcgctc cggctgcacc acgattccgg cgtgcactcc ggcggcaaga
     4261 accacctgcc gccgcgcgtc ccggcctctc ggcagcgacg acacggacag cgcgatgttc
     4321 tcctccacgg tgagcgccgg caccagccgc aagtcctgga acaccatgcc gatgccgagt
     4381 gcgcgtgcgt cggacggggc gtcgaggcgg acctcacgcc cgtcgacgag gatggcgccc
     4441 tcctgcgggc ggttgacgcc gtacaggacc ttcatgaggg tgctcttgcc ggcgccgttc
     4501 tcgccgagca gcgcatgcac ctcgccccgc tggatgtcca ggttcactgc ccgattggcg
     4561 accacgtcgc cgaatcggac cgtcacgtcg acggcttgga ggacggccgg cggcgccgtc
     4621 atggcgcgta ctcctcgcgg agtcccggca ggtcgaggcc cgcgtgctcg acgaggccgt
     4681 gggcgagggc gtaggcacgg acgtagcggt ggccctcgag gatgtgcgcg tgcatgaggg
     4741 cgaccgccag gtcggggtcg ccggccaaga tggcgcgcgc gatcccggca tgctgctcga
     4801 acgtgcggga cacgcgctcg gcgtcgatga actcgcgcat gcgtatggcg tggatgcggc
     4861 ggttgatcgt ccgcagggtc tcgagcagca ccgaaccgcc cgccgcggac gccagcgcct
     4921 cgtggaagtc ctcgtccgcc gagatgagcg tgccgtcggc gtccgcgggc ggtggggcct
     4981 cttgcggact gcgtgaccac gtcgcgagaa ggtcctcgac ggtttcgcga tccccgacgc
     5041 cgcccgcgag gcgccgtacg gcgtgggtct ccacggcgat gcggaccgca tagaggtcgt
     5101 cgaggtcgga caggagaagc cgcgtgacgc gatacccggt gtgcggggcg tactccagga
     5161 gtgcctcttg cgccagagac ctcagcgcct cccgcaccgg tgtgcgggac acgccgtaac
     5221 ggcgggccag ccccttctcg gtgaggcgca tgcccgcggg aagctggttc gtcgcgatgt
     5281 cccgcctgat gcgccccgtg agcgcctcgg tccgtgtcgc gccatccgtg tcgtcttgca
     5341 tgcaacgaga aggtaggcag gcgctgtttc ccgcccgttt ccgcgaggca acatgtccaa
     5401 gtcaatcctg ttgtgtgcaa tggggccgtg ccggaggtgg gcctgtcggc cttccttcgc
     5461 cggaggagtt cccgcgagct tcgaggtggt caggagcggc gcaggtcctc ggcgcgccag
     5521 ggcgccccgt ccgcgagcag ctccgggtcg ccggtgagca ggaggccgtc cgccgccacc
     5581 gccgttgcgg ccgcgaacgc gtcggcgtag gacagccggt aggacgcctt caggcgcgcc
     5641 gcgtcgagca cgcggccggg ggacggggct tccagggtca gccggtggcg caggtcgcgc
     5701 accacctgat cggcttcgcg cgcgccgtgc cttcgggcaa cgacgtaatg gacctcgccc
     5761 aggttgaccc agctcatgac ggggcggccg cgtcggagca ggccggcgac ccggcttgcc
     5821 gcgggctccg cgccgtggag gtacgccact accgcccagg cgtcgaggac gcgggtcacc
     5881 accgctggtc gcgcgtgcgc tcctcggccc gctccgcggc gaggacgccg agcaggtcgg
     5941 agccggcgaa gcggcctttc agcgggcgtc gtgagcgcac cggtgccagc gccacgtgcg
     6001 cgccctcgcg ccagaaggtc acctcgtcgc ccggcgcgat gccgagctct tcgcgcagct
     6061 ccttgggaat cacgacctgc cccttgggtc cgacgcggtg agtcatactg tcgagtataa
     6121 ctccgcgtgc cgttggtgtc agcgacaggc tggcacatcg ccgccgaacg cgtcagacgt
     6181 tttccacacg ctcctcgcct gcgtagacgt tcatgcgtcc ctcgcgcagg aagccgatca
     6241 gcgtcatgcc ggtctcgcgc gcgaggtcca ccgcgagcga ggacggcgcg gacacggcgg
     6301 ccaccagcgg gatcccggcc aggagcgcct tctgcgcgat ctcgaacgcg acgcgccccg
     6361 acaccatgag catgtgcgag cgcagcggaa ggcgcccctc ggtggcggcc cagccgatca
     6421 ccttgtcgac ggcgttgtgc cgcccgacgt cctcgcgcag gcacagcagc gtcccgcccg
     6481 cgtcgaacag gccggccgcg tgcaggccgc cggtgcggtc gaacacctcc tgggcggcgc
     6541 gcagcttgcc gggcagcccg tcgagcagcg ccgccggcac gcgcagcggg tcgtcgtgca
     6601 ctggggtggc ccgcctgcgc acggcttcca gcgacgcggt gccgcagatg ccgcaggagg
     6661 acgtcgtgta cacgttgcgc ttgagctcgg gcgcgaccgt cacgccgccg cgcagatgca
     6721 ggtccacgac gttgtaggtg ttcagcccgg tctccggatc ctcgccggcg cagtagcgca
     6781 tggtctccac cgcgtgcgcg tcggcgacga tgccctccgt caggcagaag cccagggcga
     6841 gctcgaagtc gtgcccgggt gtgcgcatcg tcaccgacac cgcctcgccg ctgacgcgga
     6901 tctccatcgg ctcctcggcg gccagcgcgt cccatgcccg ggtcgcctcg ccgtcgcgca
     6961 gcttgagcac cggggtcttc tcgatgcgct tgcgcgcgct cacgcgctgt cccgcaactg
     7021 ctcgatgagc gcgcgcagat gcgcgatgcg cccggcctcg tcgagctcgg cgacggccag
     7081 ggccgcggcg taggcggtga gcggggccag ccgccgctcg cgggtgtgcg cgacgacgcg
     7141 cgtgagatcc agcacggcgt cggcctcggc gtcgctcagc ggcaccgtgc cggcggtcgc
     7201 ggccaggtcg cggaagaacg gttcgacgga gtcttcggcc atggccgaat gctagggggg
     7261 cggatgcgag gccgcctcac agccgcccga ccagggcgag cggatcgtct acgacgtcgt
     7321 cggcgccggc gtcgagcagc cggtcgtcgc cgaagccgcc cgtacggacc gcgaggcagc
     7381 gcatgccgac cttgatcgcg gcctcggcgt cccacggtga gtcgccgacg agcgtcgcgc
     7441 ggtccgggtc cacaccgagc tcctggcagg cggccagcag caggtccggc gcaggcttcg
     7501 gcgagtcgac gtcgccggcg tcggtggagt ccaggtcctg ccgccccagt gcggccagca
     7561 gcgccttgcg cacgggcggc ttggccgacg tcgcgatggt gaagggcacc ttccgtcgct
     7621 ccaggtcctc caccagctcg cgggcgccgc gcgtgggtcg cagctcgtcc gcgcgctcca
     7681 ggaacaggcg gtggtgggcg tcggacagct cggactgctg ctcggtgtgg cggccgagca
     7741 gccacggcac caggcgatcc gagcccatgc cgatgccggc gtggacgcgc cacgccggca
     7801 cgtcgaggcc ggcctcgcgc agcgcctgct gccacgtctg cacgtgcagg tacacggagt
     7861 cgaccagggt gccgtccagg tcgagcagga cggcggcgat gtagggggtg ccggtcatgg
     7921 cgccaggagt ccccgcgcgg ggcggccgct atgctcgcgc ggcgatgacc gaactggacg
     7981 acgtccgagc ccgcgccgag cgctggctgg cgcatgatcc cgaccccgac acggtcgccg
     8041 agttgcgggc gctgctggcc gacgccgagc cgacgctgct gaccgcccgc ttcggggagc
     8101 gcctgccgtt cggcaccgcc ggcatccgcg gcgcgatcgg cgtgggcccc gcggcgatga
     8161 accgcctggt ggtgcgccag gtcagcgccg gcctggcgcg gcggctgctg gccgagccgg
     8221 acgcctcctc gcgcggcgtc gtggtgggtc aggacgcccg ccacaagtcc gacgagttcg
     8281 cggcggacgc ggccgcggtg ttcgccgcgc acgggctgcc cgtgcaccgc ttcgacgggc
     8341 cggtgccgac gccgctggtg gccttcgcgg tgcgcatgct gggcgcggcc gccggggtgc
     8401 aggtcacggc ctcgcacaac cccgccacgg acaacggcta caaggtctac tggggcgacg
     8461 gcggccagat caccgcgccg ctggacaccg agatcgccgc ggagatcgac ggcatcgccg
     8521 agccctggga cgtcgcgctg gccgagccgg gcgacagcct gctgggcgag gtcccgccgg
     8581 gcgtcgggga gagctacctc aagctgacgc tggaccagcg cgtggccccc ggcgcgaccg
     8641 ccctgcgcat cgtgtacacg ccgctgcacg gcgtggcgtg ggcgacggtg cgggagctgc
     8701 tgtcccgggc ggggtacggc gacgtggacg tggtcgccga gcaggccgag cccgacccgg
     8761 actttccgac ggtcggcttc cccaatccgg aggagccggg ggcgctgcac ctggcgatgg
     8821 cgctggccgc ccggcgcggg gccgacctgc tgctggccaa cgacccggac ggcgaccgca
     8881 tcgccgcggc ggtccccgaa ccggacggcc gctggcgcgc gctgtccgga gacgagatcg
     8941 gctgcctgct ggccgaccac gtgctcacct acggcggcgg cggcgacgag cgcgtggtgg
     9001 cgaccaccgt ggtgtcctca cggctgctgt cgcgcatcgc ggcggaccac gacgtcggtt
     9061 acgcggagac gctgacgggc ttcaagtggc tcgccgcgga ggcggcggcg gcggaggctc
     9121 ggggtgggcg catggtgctg gcc
//
LOCUS       DATMKC010000093         4792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_253554, whole genome shotgun sequence.
ACCESSION   DATMKC010000093 DATMKC010000000
VERSION     DATMKC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253554"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VNU01_02710"
     CDS             <1..1266
                     /locus_tag="VNU01_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH31564.1"
                     /translation="GLESVYVHLNNDSPGTDDGAGGPEWAYAPGIREGVRVERGQWIG
                     YVGDSGNAESTGSHLHFELHDPELDDPCLDGYRNPTWMNPYPSLVGAWDRGDVPEPPA
                     GDVTRLAGSNRVLTSVVIARRFDPGVRAVIVVPEGSHAEALVAAPLAGLLDAPILLAG
                     TAGPSEEALAEIRRLGAGNAYVLGREDQLAPATEALLREAGVANVARIAEPDPFALSA
                     RVAEEVRSYSEAPKLERVFLAVGDHPEPSRAWPDALSAGALAALTKAPVLLTRSGELP
                     EAVRDALRDLAPPLVQVIGGPGAVADGVEAAAREAAGGQSARLAGSNRFATSVAVADE
                     AVRLGLSAPRAWLATGHAFPDALAAGPAAAREGAALILVDGREPGGAPEPEEWLRGRG
                     VSDAIAVGGHAAITEEVRLHLARVLTERH"
     gene            1405..2403
                     /locus_tag="VNU01_02715"
     CDS             1405..2403
                     /locus_tag="VNU01_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWH31565.1"
                     /translation="MARGRPPREEIYARLETQIDELWQRMGGLPNPLEAADIWTGIWF
                     QEAHHSTAIEGNTLVLKQVETLLAEGRAVGDKQLKEYMEVRGYADAAKWVYGQAIEPG
                     EWTNGELLSLTEVRRIHEMAMTPVWNVAPPKEATPSERPGAFRQHEIEPFPGGMQPCP
                     WVEVPALMADWVEAVCNVDPQATNFPEQVAAVHCWFEQIHPFLDGNGRAGRLVLNLIL
                     VRLGYPPAIIYKNQRTAYLRALRRGDQGEFGALGEFIARAVLDNLYKFVVPAVAGPAR
                     LVPLAALATNEISAGALRVAAIRGRLQATKGPDGQWRSCRNWVDDYTKNRFRRGAA"
     gene            complement(2407..2907)
                     /locus_tag="VNU01_02720"
     CDS             complement(2407..2907)
                     /locus_tag="VNU01_02720"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWH31566.1"
                     /translation="MQFGRYYEDFEVGDVYKHWPGKTITEHEDHLFCQITYNQHPLHS
                     DRNYAEKATHFGKNVVVGNLVYSVVLGQSVPDVSGRAIANLEIEYLKHANPTFHGDTI
                     YSETKVLEKKLTSKGDRGIVKVKTIGYKQDGTVVCEFQRKVMVPLKENAGEEFPGRPE
                     PKAEDW"
     gene            complement(2967..3587)
                     /locus_tag="VNU01_02725"
     CDS             complement(2967..3587)
                     /locus_tag="VNU01_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWH31567.1"
                     /translation="MRELDALRAECERIEATLATVSADAWERPALGEWSVHKLCVHLT
                     RGVGRLAAYLGQPVEGSAVKDRVSYFQYDPAEVAPGVAARTAREAESLPAADVPQAFA
                     AAWRDSVAKASEAGRDRIMATPFGAMHVQEYSATRVLEAVVHHMDLRRALDRPADPDP
                     QAADITVEILEGLLDGPRPRNLGRDRFVLVATGRIPHDDPRFPVLG"
     gene            3707..>4792
                     /locus_tag="VNU01_02730"
     CDS             3707..>4792
                     /locus_tag="VNU01_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31568.1"
                     /translation="MDVSLDQARTLIDAARAVVDGAAARLAQASAKDGKLDIGLMDRE
                     QILAYDLSSLASQVTAAEEMLDYGEQGELERMLTVGFAGEVVADLAGRMQGRAEAWAA
                     GDANPLDDAKVQDALAAARSPELREAIAARVLRNPELPRHLDEEMSMVRETFRDFAEK
                     KVKPVAEHVHRTDADIPDDIISELAGMGCFGLSIPEDFGGFAAPTSAGHSENDFMSMV
                     VVTEELSRGSLGVAGSLITRPEILSKALVKGGTDEQKQQWLPKIAAGELMCGVAVTEP
                     DFGSDVAGLKVQAVSDGDFYVVNGTKTWCTFGGRANLLMLLARTGAPEDKHKGLSLFV
                     VEKPSYPGHEWSHDGEHGGRMDARAIGT"
BASE COUNT          686 a         1676 c         1730 g          700 t
ORIGIN      
        1 ggcctggagt cggtgtacgt gcacctcaac aacgactcgc cgggcaccga cgacggcgcc
       61 ggcgggccgg agtgggcgta cgcgccgggc attcgcgagg gcgtccgggt ggagcgcggg
      121 cagtggatcg gctacgtcgg cgactccggc aacgccgagt ccacgggatc gcacctgcat
      181 ttcgagctgc acgaccccga actcgacgac ccgtgcctgg acggctatcg caaccccacg
      241 tggatgaacc cctatcccag cctggtcggc gcgtgggatc gcggcgacgt gccggagccg
      301 cccgcggggg acgtgacgcg cctggccggc agcaaccgcg tgctcacctc ggtcgtgatc
      361 gcgcgccgct tcgacccggg tgtgcgcgcg gtgatcgtcg tgccggaggg cagccacgcc
      421 gaggcgctgg ttgcggcgcc gctcgcgggg ctgctcgacg ccccgatcct gctggcgggc
      481 accgccgggc cgtccgagga ggcgctggcg gagatccgcc gcctgggcgc gggcaacgcc
      541 tacgtgctcg gccgtgagga ccagctcgcc ccggccaccg aagcgctgct gcgcgaggcg
      601 ggcgtggcca acgtcgcgcg catcgccgag ccggacccct tcgcgctgtc ggcgcgggtc
      661 gccgaggagg tgcgctccta ctccgaggcg cccaagctgg agcgcgtgtt cctggccgtg
      721 ggcgaccacc cggaaccctc gcgcgcgtgg ccggacgcgc tgtccgccgg tgcgctcgcg
      781 gcgctcacca aggcgccggt cctgctgacg cgctcgggcg agctgccgga ggcggtgcgc
      841 gacgccctgc gcgacctggc gccgccgctg gtccaggtga tcggcggccc gggcgcggtg
      901 gccgatggcg tggaggcggc tgcgcgcgag gcggcggggg gccagagcgc gcggctggcc
      961 ggcagcaatc gcttcgcgac ctcggtggcg gtggccgacg aggccgtgcg cctggggctg
     1021 tcggcgccgc gcgcgtggct ggcgaccggc cacgcgttcc ccgacgccct ggcggccggc
     1081 cccgccgccg cccgcgaggg cgcggcgctg atcctggtgg acggcaggga gccgggcggt
     1141 gccccggagc cggaggagtg gctgcgcggc cgcggcgtgt ccgacgccat cgccgtcggc
     1201 ggccatgccg ccatcaccga ggaggtgcgc ctccacctcg cccgcgtgct gacggagagg
     1261 cactgagaat cctcgcaggg tggccgcctg aatcacaccg ctgtcatccc tagggtacgg
     1321 tcgaattcgt tggcgtggct gcgtacactg atcttgtacg gaacaattcg atagccggaa
     1381 cgcgtacaac acctggagcg atcagtggca cggggacgac cgccgcggga ggagatctat
     1441 gcgcgtctgg agacacagat cgacgagctc tggcagcgta tgggcgggct cccgaaccct
     1501 ctcgaggcgg ccgatatctg gaccgggatc tggttccagg aggcgcatca ctccactgcg
     1561 atcgaaggca acacactcgt cctgaaacag gtcgagacgc tgctcgccga aggccgtgcc
     1621 gttggggaca agcaactgaa ggagtacatg gaggtccgtg gctacgcgga cgccgcaaag
     1681 tgggtgtacg gccaggccat cgagcctgga gagtggacca acggcgagtt gctcagtctg
     1741 accgaggtcc ggcgcatcca tgagatggct atgacgccgg tgtggaatgt cgcgcctcca
     1801 aaagaagcca cgccctccga gcgcccgggc gctttccgcc aacacgagat cgagccgttt
     1861 cccggaggga tgcagccctg tccgtgggtc gaagtgccgg ctctgatggc ggattgggtc
     1921 gaggcggtct gtaatgtcga cccgcaggcg acgaacttcc ccgagcaggt ggccgccgtc
     1981 cactgctggt tcgagcagat ccaccccttc ctcgacggga atggaagggc cgggcggctg
     2041 gtgctcaacc tcatcctggt gcgcctgggc tacccgcccg cgatcatcta caagaaccag
     2101 cgcactgcgt accttcgtgc gctcagaaga ggcgatcagg gcgaattcgg ggcgctcggc
     2161 gaattcatcg ccagggccgt gctggacaac ctctacaagt tcgtcgtccc tgcggtcgcc
     2221 ggacccgcgc gactcgtccc gcttgctgcg ctggcgacga acgagatcag cgccggtgcg
     2281 ctgcgagtgg ccgccatacg gggacggttg caggccacca aggggccaga cggccagtgg
     2341 cgcagttgtc gcaattgggt cgacgactac acgaagaatc gctttcgccg cggcgccgcc
     2401 tgagccctac cagtcctcgg ccttgggctc cggccggccg gggaactcct cgcccgcgtt
     2461 ctccttcagc ggcaccatca ccttgcgctg gaactcgcac accaccgtgc cgtcctgctt
     2521 gtagccgatg gtcttcacct tcacgatgcc gcggtcgccc ttggaggtga gcttcttctc
     2581 cagcaccttg gtctcggagt agatcgtgtc gccgtggaac gtcggattgg cgtgcttgag
     2641 gtactcgatc tccaggttcg cgatcgcgcg cccggacacg tccggcacgc tctggccgag
     2701 caccacggag tacacgaggt tgccgaccac cacgttcttg ccgaagtggg tcgccttctc
     2761 ggcgtagttg cggtcggagt gcagcgggtg ctggttgtag gtgatctggc agaacaggtg
     2821 gtcctcgtgc tcggtgatcg tcttgccggg ccagtgcttg tagacgtcgc cgacttcgaa
     2881 gtcctcgtag taccgcccga actgcatcct ggctctccct tgtcccgtga ccgcgtgggt
     2941 cgctcgaccg gggattctcg cgcaggtcag cccaggaccg gaaatcgggg gtcgtcgtgg
     3001 gggatccgcc cggtggccac caggacgaag cggtcgcggc ccaggttgcg cggccgcggc
     3061 ccgtccagga gcccctccag gatctccacc gtgatgtccg ccgcctgcgg gtcggggtcc
     3121 gccggccggt ccagcgcccg gcgcaggtcc atgtggtgca cgaccgcctc gagcacgcgg
     3181 gtggcgctgt actcctgcac gtgcatggcg ccgaagggag tcgccatgat ccggtcgcgg
     3241 cccgcctcgg aggccttcgc gacgctgtcg cgccaggcgg ccgcgaacgc ctgcggcacg
     3301 tccgccgccg gcagcgactc ggcctccctc gcggtacgcg ccgcgacgcc cggagcgacc
     3361 tcggccgggt cgtactggaa gtaggacacc cggtccttga cggcgctgcc ctcgaccggc
     3421 tggccgaggt aggcggccag gcgcccgacg ccgcgcgtca ggtgcacgca cagcttgtgc
     3481 acgctccact cgcccagggc cggccgctcc cacgcgtccg cgctcacggt ggccagcgtg
     3541 gcctcgatcc gttcgcattc ggcccgcagc gcatccagtt cccgcatgcc gtcagcgtag
     3601 ggcggacgcg ccgcctgcgc ccggtccacg cgcgtgcgga ttggctcccc ggcgtcgcgc
     3661 agcgttcaat gtggcgaccc ttccgtccga ccctctagga gctccggtgg acgtctcgct
     3721 cgaccaggcc cgcacgctca tcgacgccgc acgcgccgtg gtggacggcg ccgccgcgcg
     3781 cctggcccag gccagcgcca aggacggcaa gctggacatc ggcctgatgg accgagagca
     3841 gatcctggcc tacgacctgt cgagcctggc gagccaggtc accgccgccg aggagatgct
     3901 ggactacggc gagcagggcg agctcgagcg catgctgaca gtgggcttcg ccggcgaggt
     3961 cgtggccgac ctggccgggc ggatgcaggg ccgcgccgag gcgtgggcgg ccggcgacgc
     4021 caacccgctc gacgacgcca aggtccagga cgcgctcgcc gccgcgcgct cgccggagct
     4081 gcgcgaggcc atcgcggcgc gcgtgctgcg caacccggag ctgccgcgcc acctcgacga
     4141 ggagatgtcg atggtgcgcg agaccttccg cgacttcgcc gagaagaagg tcaagccggt
     4201 cgccgagcac gtgcaccgca ccgacgccga catccccgac gacatcatca gcgagctcgc
     4261 cggcatgggc tgcttcgggc tgtcgatccc ggaggacttc ggcggcttcg ccgcgccgac
     4321 cagcgccggc cacagcgaga acgacttcat gtcgatggtg gtggtcaccg aggagctgtc
     4381 gcgcgggtcg ctgggcgtcg cgggctcgct gatcacgcgg ccggagatcc tgtccaaggc
     4441 gctggtcaag ggcggcacgg acgagcagaa gcagcagtgg ctgcccaaga tcgccgcggg
     4501 cgagctcatg tgcggcgtgg cggtcaccga gccggacttc ggctccgacg tcgccggcct
     4561 caaggtccag gcggtctccg acggcgactt ctacgtcgtc aacggcacca agacgtggtg
     4621 cacgttcggc ggccgcgcga acctgctgat gctgctggcg cgcacgggcg ccccggagga
     4681 caagcacaag ggcctgagcc tgttcgtggt ggaaaagccc agctaccccg gccacgagtg
     4741 gtcgcacgac ggcgagcacg gcggccgcat ggacgcgcgc gccatcggca cc
//
LOCUS       DATMKC010000094        13706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_16747, whole genome shotgun sequence.
ACCESSION   DATMKC010000094 DATMKC010000000
VERSION     DATMKC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16747"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VNU01_02735"
     CDS             <1..397
                     /locus_tag="VNU01_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWH31569.1"
                     /translation="FVVLSSRMAAATGADKTSMVVFFGEDRPGLLLRILDEFALRGIN
                     LTKIESRPTKQQLGEYCIFIDAAAHPVEPRMAEALRSVHRHVAELRILGAYPRADGVA
                     SAAAPADSEPAYREAAAWYTGLLEQMDPS"
     gene            394..1797
                     /locus_tag="VNU01_02740"
     CDS             394..1797
                     /locus_tag="VNU01_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HWH31570.1"
                     /translation="MTHGHANADEEGDDPVVHHRGLLLIVALIGLLGVVGMAVPPPSD
                     EFSALGPAAPDELAPDSLLLDGRITGIEVADIAGDDPTLLPGALHLDVTVVLEDGGEV
                     TFPMTDDTGDTFQVGQRVRVVEIPAQDGRPTYYISDFQRSRPLLILLGLFLAAVIWFG
                     RLQGLRALLGLGVTLALISGSLVPSIMQGKNPVLAALSTAAVIMIVTLYLSHGFHPKT
                     TAAAIGTAAALALTGLLSWVFVEAAAITGMTSEEARMATFSVGGLSLKGLLLAGIIIG
                     GLGVLDDVTMSQSSTVMQLRRANPRASFAELMRGGIAVGRDHIAATVNTLFLAYAGAS
                     LPLLLLFTTSQDSLGTIVTSEVVAVEVVRTLVGSVGLIAAVPLTTALAAALVPRVSTA
                     SAPATASAPATASAPVRVQSQSDDSESAPGEGGGSQDAEVVRPAALRHDDESDEEWEQ
                     RLRDAYRLPPRGPGGTD"
     gene            1906..2415
                     /locus_tag="VNU01_02745"
     CDS             1906..2415
                     /locus_tag="VNU01_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31571.1"
                     /translation="MLPGWVQRHLAVAGLSLVTGAAAIGLAVVGVGPLGQVAASVHGA
                     GAALSRGVLSVVMPMDPPAQDAEPAAEERERAPEAAPADQPVESQGESPEPEPEPEEP
                     APSAPVIGAERPDTEGLLAPAGSVLDGDVVDSGADDVEASAGGSGQEPDAAERLFKPV
                     QDLTTGALR"
     gene            2426..3685
                     /gene="serS"
                     /locus_tag="VNU01_02750"
     CDS             2426..3685
                     /gene="serS"
                     /locus_tag="VNU01_02750"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWH31572.1"
                     /translation="MIDIRLLRDDPDGVAALMARRGVPREDIDHIVSLDAERRGLIAR
                     GDELRAEQKARSREIGSAKPDERQALIDAVQSVSAQLDELEPLQAASEQRMLSALARL
                     PNLPHPEAPDGDTDADAVELFTFGDKPAFDFPVRDHLEIGEALGIIDIPRAVKVSGSR
                     FSFLLGQAVLLELALVRYTLDLLIQEGFTPVIPPVLTRAEALYGTAFLPFGEEQLYRI
                     PEDELYLVGTSEVPLAGYHMDEILDLDALPARYGGFSTCFRREAGTYGKDTRGMFRVH
                     QFDKVEMFSYVRPEDSEAEHLRLRDLQVRILSGLGLHGRVVDIPVGDLGASATRKYDV
                     EVWLPGQEAYRELTSASNCLDYQARRLRTRYRTEDGENEMVHTLNGTACAVGRTIIAL
                     LETHQLADGTVAVPQALRPYLGADRIG"
     gene            3781..3921
                     /locus_tag="VNU01_02755"
     CDS             3781..3921
                     /locus_tag="VNU01_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31573.1"
                     /translation="MKRLRRRPAPVVFCGEGNKVCSRRAVARELREQSKASAAFGHLA
                     WK"
     gene            3932..4327
                     /locus_tag="VNU01_02760"
     CDS             3932..4327
                     /locus_tag="VNU01_02760"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HWH31574.1"
                     /translation="MSRVQLALNVSDLDRAVAFYSRLFGAEPAKRRPGYANFAVEDPP
                     LKLVLIEGEAEGGTLNHLGVEVADTAAVAQAAGRLSSEGLATSEEQGVECCYAVQDKV
                     WVSDPDGAPWEVYAVLADSPVLVKAQGCC"
     gene            complement(4496..4741)
                     /locus_tag="VNU01_02765"
     CDS             complement(4496..4741)
                     /locus_tag="VNU01_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31575.1"
                     /translation="MDDSVAVAAELERLRLELENRDRVAMRMAAKARRLEDAIALHRR
                     RKSGEADDVAFLDPADIDALLWQELEPTDERHLLDSA"
     gene            complement(4881..4970)
                     /locus_tag="VNU01_02770"
     tRNA            complement(4881..4970)
                     /locus_tag="VNU01_02770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4932..4934),aa:Ser,seq:gct)
     gene            5069..5158
                     /locus_tag="VNU01_02775"
     tRNA            5069..5158
                     /locus_tag="VNU01_02775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5105..5107,aa:Ser,seq:tga)
     gene            5241..5798
                     /locus_tag="VNU01_02780"
     CDS             5241..5798
                     /locus_tag="VNU01_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH31576.1"
                     /translation="MRGVALDEQELVRRARGGDLAAYEDLVRAHQAAAARLAAILAGP
                     ADAEDIAQTAFVKAHAALSRFRHDAPFRPWLLRIVANEARNTVRGRQRRAARDTRWSQ
                     EPIVPQPDVEQHLLARERDAALWAALDRLGHADRAVLACRYLMELSVAETAAALRWPP
                     GTVKSRQSRALRRLERLLAGQEVAR"
     gene            5795..6550
                     /locus_tag="VNU01_02785"
     CDS             5795..6550
                     /locus_tag="VNU01_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31577.1"
                     /translation="MSVVEERLRAAGAALADAPVPDVSGRVMERLESGRSRRGPALPR
                     TLAAAAAALVLLAGTVLVFPSAGAALADLLGLPGLRIVFERRMPPVHGGLALGEAVSL
                     AEARAATAFPLAFPKALGRPDEVWLDRHAVPGGQVTLLWRPGPGLPASGPHRLGALVT
                     VLPGRLDAGALAKSLWQEDTELTAVRVGGQEGWWVSGADHALVYFDAEGGVRADRSRL
                     AGPTLLWQDGEVLYRLESGLSLARALEVAASVR"
     gene            6641..7159
                     /locus_tag="VNU01_02790"
     CDS             6641..7159
                     /locus_tag="VNU01_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWH31578.1"
                     /translation="MRTRTLLALLLLTLLLPAAPASAGGGRCHEGFTDGQGPKVTTSG
                     GCFLPTVVRVAPGDTVTWRSGDGVGHNVVTSAGFGGDLPPKTPLTHTFAEPGVYPYVC
                     TLHPGMVGAVVVGDGIPQKASFVPASAAVEAGPADGAPRWALAAALGLLMVLALARAG
                     MRKRRAAGPAPS"
     gene            7264..8904
                     /locus_tag="VNU01_02795"
     CDS             7264..8904
                     /locus_tag="VNU01_02795"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWH31579.1"
                     /translation="MQGLMQDTPLTLQMVLRRAGTLYGDKVIATKTADGVRRRTFAET
                     VERVHRLASALRDLGIGPGDRVATFGWNSDHHLEAYLAVPCMGAVLHTLNIRLHPDQV
                     AWIADHAQDSVVLVDASLAEAWSQVKDVESIRHVVVMDDTGQGAGVVPGALDYEELIA
                     GASPAFDWPELDELSAAAMCYTSGTTGDPKGVVYSHRSSTLHALASLFADNLALSERD
                     TVLPIVPQFHVNAWGIPYAALMTGASVAMPSRFMDPASVAALVRETGTTISAAVPTVW
                     LGVLQALQGGQVDAADLATLNRIVIGGSACPESLMRAYDELGVNVVHAWGMTETSPLG
                     TVARVKTTVPQDQVWSRRLTQGLPAPTLDVRIVDDAGVPQPWDGESPGELEISGPWIA
                     AAYYDPQAPDGRGGEDRFADAEGRRWLRTGDVAVIHPDGYVQLVDRVKDLVKSGGEWI
                     STVELENLLIAHPKVAEAAVIAVPHPKWDERPLACVVKADDSVTGAELLEYLAGHVAK
                     WQVPDDVVFIDEVPKTSVGKFDKKVLRSQFGQYQLPES"
     gene            8979..9404
                     /locus_tag="VNU01_02800"
     CDS             8979..9404
                     /locus_tag="VNU01_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWH31580.1"
                     /translation="MTMPPTPAHAPSRHYERLTHQECLDRLQRGGVGRLAFSDGPAPV
                     IVPVNYGMLGDTPVLRTAFGTVIHGLAEGEGVALEIDEVDPITHLAWSVLVRGRIERV
                     TDPADLLACEALRIEAYAPGRRTHWRRIVPIRVTGRVIR"
     gene            complement(9394..10284)
                     /locus_tag="VNU01_02805"
     CDS             complement(9394..10284)
                     /locus_tag="VNU01_02805"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HWH31581.1"
                     /translation="MEVLRTPDERFSALPDWPYEPRYAEVGDGLRAHYVDEGPRDGEP
                     VLLLHGEPTWGYLYRHMIPPLAAAGLRVVVPDLIGFGRSDKPAAQADYTYARHVEWMR
                     ALLFDHLSLSGVTLFGQDWGGLIGLRLVGEHPDRFARVVAGNTFLPTGDHEPSEAFLA
                     WQRFSQETPEFPIGRIVNGASKRELSEAEVAAYDAPFPEESYKAGARRFPMLVPTSPE
                     DPAAEDNRKAWETLSAWDKPFLCTFSDGDPITAGGDRVFRKLVPGTAGMAHRTVSGGH
                     FLQEDSGPELAAAIVELISG"
     gene            complement(10294..11193)
                     /locus_tag="VNU01_02810"
     CDS             complement(10294..11193)
                     /locus_tag="VNU01_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWH31582.1"
                     /translation="MIFFGAALYATGPVFVGASSVPGPVFSFWRLWLGVAVLGLATLV
                     WRLAGGRLPPLAAWRWPLLAGLSFGTHQVLFMTAIKATSVTTVALIGTISPIIVAIAA
                     VPLFGERMGARFRLWTVLAMAGAAVVVTGASTGPAGDLPGTVMAVLNVVFFAGFFLVS
                     KVSRDHIPVVPFLLGVMVTAAIWVSLFLLATGQAVRGMDSHDYLYALAVAAGPGALGH
                     FVMTWPLRYVAANVPPVVRLSQPVISGTLAWAFLGEAIGRSHVLGGALTLLGVAGAML
                     SPSGRELPGTALEEDEPAPELPG"
     gene            11389..11462
                     /locus_tag="VNU01_02815"
     tRNA            11389..11462
                     /locus_tag="VNU01_02815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11423..11425,aa:Arg,seq:acg)
     gene            complement(11566..12099)
                     /locus_tag="VNU01_02820"
     CDS             complement(11566..12099)
                     /locus_tag="VNU01_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31583.1"
                     /translation="MSRRERREPGLLQVRLFGPPRIIRNGTEMPMPPRKPATALKILA
                     LRGGLVHQHKLAGWLWPDEDPSIALPRIRDVLAKARRATGAHIQQRNEAIRVIDQLDC
                     DIVKFIHLASRAIARIDSPQEVNRLAIQAQSLWVGPPLEEDRYEPWAQTPLRQATELQ
                     RQLWKLLPDTDHRTRTD"
     gene            12654..>13706
                     /locus_tag="VNU01_02825"
     CDS             12654..>13706
                     /locus_tag="VNU01_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWH31584.1"
                     /translation="MGLYGGALSRTHAALALAIVTVLTLAGGAVVASEFGPVPVSRQP
                     APDARVNEGSPEIAVVFDEDIALVEGAGAPTIVDAAGVSRPGVTSALENPRKLLIQYT
                     GPLPDGGYTVTIPEGHVSRADLPMSTNATITWKFQVDTVASPPVVAAVDDPVNLQKQS
                     AVAVAGTSEPGSTVTVEASDGVKILVSAPGLTANQDGQWDTTLNVTDLADGPLTVSAT
                     ATDLAGNTSPRSAPRHTRKDATPPTVRLTVPAGAVSATSKPGIVLEFSESVRPTPYDG
                     EDGVRVRQGSVTLVHADSGESVEGVTGVVEAATGTLHVLYEGELDDGDYTVSVAEDTV
                     RDTAENPNDAASLDFSV"
BASE COUNT         1915 a         4748 c         5106 g         1937 t
ORIGIN      
        1 gttcgtggtg ctgagcagcc gcatggccgc ggcgacgggc gcggacaaga cctccatggt
       61 ggtgttcttc ggcgaggatc gtccgggcct gctgctgcgc atcctggacg agttcgcgct
      121 gcgcggcatc aacctgacca agatcgagtc gcggccgacc aagcagcagt tgggcgagta
      181 ctgcatcttc atcgacgcgg cggctcaccc cgtcgagccg cgcatggcgg aggccctgcg
      241 cagcgtgcac cgccacgtcg ccgaactgcg catcctgggg gcgtacccgc gcgccgacgg
      301 cgtggcgagc gccgcggccc cggccgactc ggagccggcc taccgggagg cggccgcgtg
      361 gtacaccggc ctgctggagc agatggaccc gtcgtgaccc acggccacgc gaacgccgac
      421 gaggagggcg acgacccggt cgtccaccat cgcgggctgc tgctgatcgt ggcgctgatc
      481 ggcctgctcg gcgtggtcgg catggcggtg ccgccgccgt cggacgagtt ctccgcgctc
      541 ggacccgcgg cgcccgacga gctcgcgccg gacagcctgc tcctggatgg ccggatcacg
      601 ggcatcgagg tggccgacat cgccggagac gaccccacgc tgctgccagg cgcgctgcat
      661 ttggatgtca ccgttgtgct ggaggacggc ggcgaggtca cgttcccgat gaccgacgac
      721 acgggcgaca cgttccaggt cggccagcgc gtgcgcgtgg tggagatccc cgcgcaggac
      781 ggccggccca cgtactacat cagcgacttc cagcgctcgc ggccgctgct gatcctgctg
      841 ggcctgttcc tggccgccgt catctggttc ggccggctgc agggcctgcg cgcgctgctc
      901 ggcctgggtg tgacgctcgc gctcatcagc ggctcgctcg tgccctcgat catgcagggc
      961 aagaacccgg tgctcgccgc gctgtcgacc gcggcggtga tcatgatcgt caccctgtac
     1021 ctgtcacacg gcttccaccc caagaccacg gcggcggcga tcggcaccgc ggcggcactg
     1081 gccctcacgg ggctgctgtc gtgggtgttc gtggaggcgg cggcgatcac cggcatgacc
     1141 agcgaggagg cgcgcatggc gaccttctcg gtgggtgggc tgtcgctgaa ggggctgctg
     1201 ctggcgggga tcatcatcgg cgggctgggc gtgctggacg acgtgacgat gtcgcagtcg
     1261 tccacggtga tgcagttgcg ccgggccaac ccgcgggcgt cgttcgccga gctcatgcgc
     1321 gggggcatcg cggtggggcg cgaccacatc gccgcgacgg tcaacacgct gttcctcgcc
     1381 tacgccggcg cgtcgctgcc gctgctgctg ctgttcacca ccagccagga ctcgctgggc
     1441 acgatcgtga cgtccgaggt ggtggcggtg gaggtggtcc gcacgctcgt cgggtcggtg
     1501 gggctgatcg cggcggtgcc gttgaccacg gcgctggccg ccgcgctggt cccgcgagtg
     1561 tccacagctt ccgccccggc cacagcttcc gccccggcca cagcttccgc cccggtacgg
     1621 gtgcagtccc agagcgatga ctctgaaagt gcacccggtg aggggggcgg gtcgcaggac
     1681 gccgaggtgg tgcggccggc ggccttgcgc cacgacgacg agtccgacga ggagtgggag
     1741 cagcgcctgc gcgacgccta ccgcctgccc ccccgcgggc ccggcggcac ggactagtcc
     1801 gcccgggggg ttttgggata tggcccgtga tgcgcggccg cgtcctgccg ttgtcccagg
     1861 tagcgacgcc gcagcgggtg gcgtccctgc cgagaggatc gcgtcgtgct ccctggatgg
     1921 gtccagcgtc acctggccgt ggccgggctg tcgctggtca ccggcgcggc cgcgatcggc
     1981 ctcgccgtcg tgggtgtcgg cccgctcggc caggtcgcgg cgagcgtcca cggggccggt
     2041 gcggcactga gccgcggcgt gctgtccgtg gtcatgccca tggacccgcc cgcgcaggac
     2101 gccgaaccgg ccgccgagga gcgcgagcgc gcgccggagg ccgctccggc ggaccagccg
     2161 gtggagtcgc agggagagtc gccggagccg gagcccgagc ccgaggagcc ggcgcccagc
     2221 gcgccggtga tcggcgccga gcggccggac accgaggggc tgctggcgcc ggccggcagc
     2281 gtgctggatg gcgacgtggt cgactcgggc gccgacgacg tggaagcgtc ggccggcggc
     2341 tccgggcagg aacccgacgc ggccgagcgc ctgttcaagc ccgtgcagga tctgaccacg
     2401 ggagcgctgc gctagcctct cgcgcatgat cgacatccgc ctcctgcgcg atgaccccga
     2461 cggtgtggcc gccctcatgg cacggcgcgg cgtgccgcgt gaggacatcg accacatcgt
     2521 gtccctggac gccgagcgcc gcgggctgat cgcgcgcggc gacgagttgc gcgcggagca
     2581 gaaggcccgt tcccgggaga tcggctcggc caagccggac gagcgccagg ccctcatcga
     2641 cgccgtgcaa tcggtctcgg cgcagctcga cgagctggag ccgctgcagg ccgcctcgga
     2701 gcagcgcatg ctctccgcgc tggcgcggct gcccaacctg ccgcacccgg aggcgccgga
     2761 cggggacacc gacgccgacg ccgtggagct gttcacgttc ggcgacaagc cggcgttcga
     2821 cttcccggtg cgcgaccacc tggagatcgg cgaggcgctc gggatcatcg acatccctcg
     2881 cgcggtcaag gtcagcggca gccgcttcag cttcctgctc gggcaggcgg tcctgctgga
     2941 gctggccctg gtccgctaca cgctggacct gttgatccag gagggcttca cgccggtgat
     3001 cccgccggtg ctcacgcgcg cggaggcgct gtacggcacg gcgttcctgc cgttcgggga
     3061 ggagcagctg taccggatcc cggaggacga gctgtacctg gtgggcacgt ccgaggtgcc
     3121 gctggccggc taccacatgg acgagatcct ggacctcgac gcgctgccgg cccgctacgg
     3181 cggcttctcg acctgctttc gccgcgaggc cggcacgtac ggcaaggaca cgcgcggcat
     3241 gttccgcgtc caccagttcg acaaggtgga gatgttcagc tacgtgcggc cggaggactc
     3301 cgaggccgag cacctgcgcc tgcgcgacct ccaggtgcgc atcctgtccg ggctggggct
     3361 gcacgggcgc gtcgtggaca tcccggtcgg cgacctcggc gccagcgcca cgcgcaagta
     3421 cgacgtggag gtgtggctgc cggggcagga ggcctaccgc gagctcacca gcgcctccaa
     3481 ctgcctggac taccaggcgc gccgcctgcg cacgcgctac cgcaccgagg atggcgagaa
     3541 cgagatggtg cacacgctga acggcacggc gtgtgcggtg gggcggacga tcatcgcctt
     3601 gctggagacc caccagctcg ccgacggcac cgtggcggtg ccccaggcgc tgcggcccta
     3661 cctgggcgcc gaccgcatcg gctgaacggg tcgagaacgc ccggccgaaa agattgacaa
     3721 ccttcgatgg agccctctat ggtccttaca tcgaagaacg tcgaatcagg aggtccggtc
     3781 atgaagcgtc tgcgccgccg tcccgcgccc gtcgtgttct gcggcgaggg caacaaggtc
     3841 tgcagccgcc gcgccgtggc ccgcgaactc cgtgagcagt ccaaggcgtc ggccgccttc
     3901 ggccacctcg cctggaagta ggaggcccgc catgtcgcgt gtgcaactcg ccctgaacgt
     3961 gtccgacctc gaccgggcgg tggcgttcta ctcccgcctg ttcggcgccg agccggccaa
     4021 gcgccgcccc gggtacgcca acttcgccgt ggaggatccg ccgctgaagc tcgtcctcat
     4081 cgagggcgag gcggagggcg gcacgctcaa ccatctgggc gtagaggtgg cggacacggc
     4141 cgcggtcgcg caggccgccg gccgcctgag ctcggaaggg ctggcgacct cggaggagca
     4201 gggcgtcgag tgctgctacg cggtgcagga caaggtgtgg gtgtcggacc cggacggcgc
     4261 gccatgggag gtgtacgcgg tcctggccga ctcgcccgtg ctggtcaagg cccagggctg
     4321 ctgctgacgg cccgatgacg tatccggaaa tagcccttca ggactagaca cggattaccg
     4381 aatagataga tgcaaccgcg cgggatgtcc ccccgtcgtc ccccgcgggc acgcgagcaa
     4441 gactcgcacg ggaggccttc ggcgtgagcc ggaggcctcc tctcgtgcgc gccggtcagg
     4501 ccgagtccag cagatggcgc tcgtcggtgg gttccagctc ctgccacagc agggcgtcga
     4561 tgtcggcggg gtccaggaag gcgacatcgt cggcctcgcc ggacttgcgc cgcctgtgca
     4621 gcgcgatggc gtcctcgagg cgccgggcct tggcggccat ccgcatcgcg acccggtcgc
     4681 ggttctccag ctcaaggcgc agccgctcga gttcggccgc gaccgccacg ctgtcgtcca
     4741 tcctgccccc gtatggcgcc gccacgacgt gccgtggcgg acggtcaatg gtgtcctggc
     4801 gcgccatggg cgatggacta gagcgatgat cggcgcgcgc agcgccggcg cgccccgtcg
     4861 caaccaggcg tgccgatgcg cggaggctga gggattcgaa ccctcgagag gggaaacccc
     4921 tcaacgggat tagcaatccc gcgccataga cctgactagg cgaagcctcc tgggtgctcg
     4981 cggtgccgga gtgctcccgg cggcgaacga cggcgagtgt atcgaccgca ccggccacct
     5041 gcgacccggc tgcctacaat cgcggcacgg aggcgtggcc gagtccggct gatggcgcag
     5101 gtcttgaaaa cctgtgagcg gttctgccgc tccgtgggtt cgaatcccac cgcctccacg
     5161 atgcccgcct gccagcggtg gaaccggacc gggcgccgcg gtgtacggat gcggacggca
     5221 tccgggcagg cggagacgac gtgcggggag tcgcgctgga cgaacaggaa ctggtccgcc
     5281 gggcgcgcgg cggcgatctg gccgcgtacg aggatctcgt ccgggcgcac caggccgcgg
     5341 ccgcacgcct ggccgcgatc ctggcgggcc ccgcggacgc cgaggacatc gcgcagacgg
     5401 cctttgtgaa ggcgcacgcc gccctgtccc gcttccgcca cgacgcgccg tttcgcccct
     5461 ggttgctgcg catcgtggcc aacgaggcgc gcaacaccgt gcgaggacgc cagcgtcgcg
     5521 ccgctcgcga cacgcgctgg agccaggagc cgatcgtgcc ccagccggac gtcgagcagc
     5581 acctgctggc gcgcgagcgg gatgcggcgc tgtgggcggc cctcgatcgg ctggggcatg
     5641 ccgaccgcgc cgtgctggcc tgccggtacc tgatggagtt gtccgtggcc gagacggcgg
     5701 cggcgctgcg ctggccgccc ggcacggtga agtcccgcca gagccgggcg ttgcggaggc
     5761 tggagcggct gctggccggc caggaggtgg cccgatgagc gtcgtggagg agcggctgcg
     5821 cgccgcgggc gccgccctgg cggacgcgcc ggtgccggat gtgtccggcc gggtcatgga
     5881 gcggctggaa agcgggcgat cgcggcgggg accggcgttg ccccgcacgc tcgccgcggc
     5941 cgcggcggcg ctggtgctgc tggccggcac ggtgctggtg ttcccctcgg cgggtgccgc
     6001 cttggcggac ctgctcggcc tgccggggct gcgcatcgtg ttcgagcggc gcatgccgcc
     6061 cgtccacggc gggcttgccc tcggcgaagc ggtgtcgctg gccgaggcgc gtgccgccac
     6121 cgcgttcccg ttggcgttcc cgaaggcgct cggcaggccc gacgaggtct ggctggaccg
     6181 gcacgcggta ccgggcggcc aggtcacgct gctgtggcgg ccgggacccg gcctgcccgc
     6241 gtcggggccg catcgcctcg gcgcactggt caccgtgctg cccggcaggc tggacgccgg
     6301 cgcgctggcc aagtcgctgt ggcaggagga cacggagctg accgcggtcc gggtgggcgg
     6361 ccaggagggc tggtgggtgt ccggcgcgga ccatgcgctg gtctacttcg acgccgaagg
     6421 cggcgtgcgc gccgaccgca gcaggctcgc ggggcccacg ctgctgtggc aggacggcga
     6481 ggtgctgtac cggctggagt ccggcctgtc cctggcgcgc gccttggagg tcgccgcgtc
     6541 ggtgcgctga gggggaaccg gcttcctcct gcgcggaacc gggcgcgtcg cggcggtgtc
     6601 gtgaccgtga ccgcgtcaac ccggctgtga agggggaccc atgcgcaccc gcacgctgct
     6661 cgcgctgttg ctgctcacgt tgctgctgcc ggccgcaccg gcgtcggccg gcggtggacg
     6721 ctgccacgag gggttcaccg acgggcaggg gcccaaggtc accacctcgg gaggctgttt
     6781 cctgcccacg gtggtgcgcg tggcgcccgg cgacacggtg acgtggcgca gcggcgacgg
     6841 ggtcgggcac aacgtggtga cgagcgccgg attcggcggg gacctgccgc ccaagacccc
     6901 gctgacccac accttcgccg agccgggcgt gtacccgtac gtctgcacgc tgcaccccgg
     6961 catggtgggc gccgtggtcg tcggcgacgg cataccgcag aaggcgtcgt tcgtcccggc
     7021 gtcggcggcc gtcgaggcgg gccccgccga cggcgcgccg cggtgggcgc tggccgccgc
     7081 cctggggctg ctgatggtgc tggcattggc ccgggccggc atgcggaagc ggcgcgccgc
     7141 cggccccgcg ccctcgtagc gccctgcctc cgctccctcc gcccgaactg gccgtggacc
     7201 gccgcggtcc caggggccaa gatgcccgcc ggagcgcgac gcgacggacg cgggagtggg
     7261 cggatgcagg gactgatgca ggacacgccg ttgacgctgc agatggtgct gcggcgggcg
     7321 ggcacgctgt acggcgacaa ggtgatcgcc accaagaccg cagacggcgt gcgacgccgc
     7381 acgttcgccg agaccgtgga gcgcgtgcac cgcctcgcct ccgcgctgcg cgatctgggg
     7441 atcggcccgg gggatcgcgt cgccacgttc ggctggaaca gcgaccacca cctggaggcc
     7501 tacctggcgg tgccgtgcat gggcgccgtg ctgcacacgc tcaacatccg cctgcacccc
     7561 gaccaggtcg cctggatcgc cgaccacgcc caggacagcg tggtgctggt cgacgcgtcc
     7621 ctggccgagg cgtggagcca ggtcaaggac gtcgagtcca tccgtcacgt ggtggtgatg
     7681 gacgacaccg gccagggcgc gggcgtcgtg cccggcgcgc tcgactacga ggagctgatc
     7741 gccggcgcct cgccggcgtt cgactggccg gagctggacg agctgtccgc ggcggccatg
     7801 tgctacacgt cgggcaccac cggcgacccc aagggcgtcg tgtactcgca ccgctcctcg
     7861 acgctgcacg cgctggcgtc gctgttcgcc gacaacctgg cgctgtccga gcgcgacacg
     7921 gtgctgccga tcgtgccgca gttccacgtc aacgcctggg gcatcccgta cgcggcgctg
     7981 atgaccggcg catccgtggc gatgcccagc cgcttcatgg acccggcctc ggtcgccgcg
     8041 ctggtgcgcg agaccggcac gacgatctcc gccgcggtcc ccaccgtgtg gctgggcgtc
     8101 ctgcaggcgc tgcagggcgg gcaggtggac gcggcggacc tggccacgct gaaccgcatc
     8161 gtgatcggcg gctcggcctg cccggagtcg ctgatgcgcg cctacgacga gctgggcgtc
     8221 aacgtggtgc acgcctgggg catgacggag acctcgccgc tgggcacggt ggcccgcgtg
     8281 aagaccacgg tgccacagga ccaggtgtgg tcccggcggc tcacgcaggg cctgcccgca
     8341 ccgacgctgg acgtgcgcat cgtcgacgac gcgggcgtcc cgcagccgtg ggacggcgaa
     8401 tcacccggcg agctggagat cagcgggccg tggatcgccg cggcctacta cgacccgcag
     8461 gcgcccgatg gccgtggcgg cgaggaccgc ttcgccgacg ccgagggacg gcgctggctg
     8521 cgcaccggcg acgtcgcggt catccatccc gacggctacg tgcagctcgt cgaccgcgtg
     8581 aaggacctgg tgaagtccgg cggggagtgg atctccaccg tggagctgga gaatttgctg
     8641 atcgcccacc cgaaggttgc cgaggccgcc gtcatcgcgg tgccgcaccc caagtgggac
     8701 gagcgcccgc tggcgtgcgt ggtcaaggcc gacgactcgg tgacgggcgc cgagctgctg
     8761 gagtacctgg cggggcacgt ggccaagtgg caggtccccg acgacgtggt cttcatcgac
     8821 gaagtgccca agacctcggt cggcaagttc gacaagaagg tcctgcgcag ccagttcggc
     8881 cagtaccagc tcccggagag ctgacggggc gggagtccgg ctcgcggcgg gactttcggc
     8941 cctgggggac cggggacgcg atgcgcagca tggacaccat gacgatgcct cccacccccg
     9001 cgcacgcccc gagccgccac tacgagcgcc tcacgcacca ggagtgcctg gacaggctcc
     9061 agcgtggcgg cgtgggacgg ctggcgttct ccgacggccc cgcgccggtc atcgtgcccg
     9121 tgaactacgg gatgctcggc gacacgccgg tgctgcgcac ggcgttcggc acggtcatcc
     9181 acgggctcgc cgagggtgag ggcgtcgcct tggagatcga cgaggtcgac ccgatcacgc
     9241 acctggcgtg gagcgtgctg gtgcgcggcc gcatcgagcg cgtcaccgac cccgcggacc
     9301 tgctcgcctg cgaggcgctg cggatcgagg cctacgcgcc ggggcggcgg acgcactggc
     9361 ggcgcatcgt gccgatcagg gtcaccggcc gcgtcatccg ctgatcagtt cgacgatggc
     9421 ggcggccagc tccgggccgc tgtcctcctg caggaagtgg ccgccgctca cggtgcggtg
     9481 cgccatgccg gcggtgccgg gcacgagctt gcggaacacc cggtccccgc ccgcggtgat
     9541 ggggtcgccg tcgctgaagg tgcacaggaa cggcttgtcc cacgccgaca gcgtctccca
     9601 cgccttgcgg ttgtcctcgg ccgcggggtc ctcgggcgag gtggggacca gcatggggaa
     9661 gcgccgggcg ccggccttgt aggactcctc cgggaacggc gcgtcgtagg cggcgacctc
     9721 cgcctccgac agctcccgct tggacgcgcc gttgacgatg cggccgatgg ggaactcggg
     9781 cgtctcctgg ctgaagcgct gccacgccag aaacgcctcc gacggctcgt gatcgcccgt
     9841 cggcaggaag gtgttgccgg ccaccacccg cgcgaagcgg tccgggtgct cgcccaccag
     9901 gcgcaggccg atgaggccgc cccagtcctg gccgaacagc gtcacgccgc tcaagctcag
     9961 gtggtcgaac agcagcgcgc gcatccactc gacgtggcgg gcataggtgt agtcggcctg
    10021 cgccgccggc ttgtcgctgc ggccgaagcc gatcaggtcg ggcaccacga cgcgcagccc
    10081 ggcggcggcc agcggcggga tcatgtgccg gtacaggtag ccccaggtgg gctcgccgtg
    10141 cagcagcagc acgggctcgc cgtcgcgcgg gccctcgtcc acgtaatggg cccgcagccc
    10201 gtcgcccact tcggcgtagc gcggctcgta cggccagtcc ggcagtgccg agaaccgctc
    10261 gtccggtgtg cgcaggacct ccatgacgcg ctcctatccc ggcagctcgg gcgccggctc
    10321 gtcttcctcc aaagcggtgc cgggcagctc ccgcccggag ggggacagca tcgcacccgc
    10381 gacgccgagc agcgtgagcg cgccgcccag cacgtgcgag cggccgatcg cctcgcccag
    10441 gaacgcccat gccagcgtgc cggagatcac cggctgggac aggcgcacca ccggcgggac
    10501 gttggcggcc acgtagcgca ggggccaggt catcacgaag tggccgagcg cgccaggccc
    10561 ggcggcgacg gccagcgcgt acaggtagtc gtgggagtcc atgccccgga cggcctgacc
    10621 ggtggccagc aggaacagcg acacccagat cgcggccgtc accatcacgc ccagcaggaa
    10681 cggcaccacg gggatgtggt cgcgcgagac cttggacacc aggaagaagc cggcgaagaa
    10741 caccacgttc agcacggcca tgaccgtgcc cggcaggtcc cccgccgggc cggtcgacgc
    10801 accggtgacc accacggccg cgcccgccat ggccaggacg gtccacaagc ggaaccgcgc
    10861 gcccatgcgc tcgccgaaca gggggaccgc ggcgatcgcc acgatgatcg gcgagatcgt
    10921 gccgatgagg gcgaccgtgg tcaccgacgt ggccttgatg gcggtcatga acagcacctg
    10981 gtgcgtgccg aacgacaggc cggccagcag cggccagcgc caggccgcca gcggcggaag
    11041 gcgcccgccg gccagccgcc acacaagcgt ggccaggccc aggacggcca cgcccagcca
    11101 cagccgccag aagctgaaca cgggcccggg tactgaggac gcccccacga acacgggccc
    11161 cgtggcgtac agcgccgcgc cgaagaagat catcgccaac ggatggcgcg ccgcgacgcc
    11221 atgtgccgcg gcacgccgcc tcgcgaccga ctctctcgct gcctctcgcg ccatgtcggg
    11281 gcagcgtgcc acacggcgcc aagcgcctcg ggtgggccga tggacggtgt gcggctcgca
    11341 aatgcggcgg gtactatcgg cgcccggtcc gccttggcga ggccgcccgc gccggtagct
    11401 cagtggatta gagcatctga ctacggatca gaaggtcggg ggttcgagtc cctcccggcg
    11461 cgctttctgc gatgtcccga gacatggtca cacccagtct cgggacatcg ttgcgtttga
    11521 acgaccggcg tcggtggccc cgagaggcca gtcacgcggc acgtgtcagt ccgtgcgagt
    11581 tcggtggtcg gtgtcgggga gcagtttcca gagctggcgt tgaagttccg tggcttggcg
    11641 gagtggtgtc tgggcccagg gctcgtagcg gtcttcttcc aggggtgggc cgacccagag
    11701 cgattgggct tggatggcca ggcggttgac ctcttggggg ctgtcgatgc gggcgatggc
    11761 gcggctggcc aagtggatga acttgacgat gtcgcagtcg agctggtcga tgaccctgat
    11821 cgcttcgttg cgctgttgga tgtgggcgcc ggtggcgcgg cgcgctttgg cgagcacgtc
    11881 gcggatgcgg ggcagggcga tggaggggtc ctcgtcgggc cacagccatc cggcgagttt
    11941 gtgctggtgg acgagcccgc cgcgcagggc gaggattttg agggcggtgg cgggctttct
    12001 ggggggcatg ggcatctcgg tgccgttgcg gatgattctg ggcggtccga acagccgcac
    12061 ttggagcagg ccgggttctc ggcgttcacg acggctcact ggcggcgctc cgcgcgcacg
    12121 ccgcgactgt ctcccttgcg ggtgtggacc gctgggcagg gagtggctgg cgccacggtg
    12181 gacggtccgg ggggatagag gggcatgggc gtcgctctgt cctacgtcct aggcattcgg
    12241 gcacgctgac gcgggtggac tggcccactt cgcgcggcca ctgaacgcgc ggattgctcg
    12301 gccctttcgg ccgagggaca agagatacaa aaaagataca tctgttttgg gatgcctaat
    12361 cgcgccaggc ccggccccca agaatggcgg tgtgggaacg tactcgcggg acgtacccac
    12421 aacgcaccag tcgcgtaccg ttgcgccaaa agcgtcgcgc cgcgctcggg ccacgaccac
    12481 cgatgcctta aatcagttgt atcgagtttg tatctcattt gtatctcccc gtcggccggt
    12541 cataagaata ggaacattat tgccgtactc ttgccagccg tggttggcgg gacccggtca
    12601 tgggacagcg ccagtgcatc gggcagcggc ggccgacggg gagacacgac gagatgggcc
    12661 tgtacggggg agcgttgagc cgcacgcatg cggccttggc actggccatc gtgaccgtct
    12721 tgacgctggc gggaggggct gtggtggcca gcgagttcgg ccccgtgccc gtaagtcgcc
    12781 agccggcgcc ggatgcgcgg gtcaacgagg ggtcacccga gatcgccgtc gtgtttgatg
    12841 aggacatcgc actcgtcgag ggggctggtg cgccgaccat cgtggacgct gcgggcgtgt
    12901 cgcggccggg tgtgacctcg gcgctggaga acccgcggaa gctgctgatc cagtacaccg
    12961 gcccgctgcc ggacggcggg tacaccgtca cgattcctga agggcatgtg tcccgggctg
    13021 atctgccgat gtcaacgaac gcgacgatca cctggaagtt ccaggtcgac acggtcgcgt
    13081 cccccccagt cgtggccgcc gtggacgacc ccgtgaacct ccagaagcag tcggccgtgg
    13141 ctgttgcggg cacgtctgag ccgggaagca ccgtgacggt cgaggcgtcc gatggcgtca
    13201 agattttggt cagcgccccc ggcctcacgg ccaaccagga cgggcagtgg gacacgacgc
    13261 tgaacgtgac ggacctggcc gacggcccct tgaccgtctc cgccacagcc accgacctgg
    13321 ccggcaacac cagcccgcgg tccgcgcccc gtcacacccg caaggacgcc accccgccga
    13381 cggtgcggct gacggtcccg gccggagcag tctccgcgac atccaagccg ggcatcgtgc
    13441 tggagttctc cgaatccgtg cggccgactc cgtacgacgg cgaagacggt gtcagggtgc
    13501 ggcagggctc cgtgacgctg gtgcacgccg actccggtga atcggtcgag ggcgtgaccg
    13561 gcgtggtgga ggcggccacg ggcacgctcc atgtgctcta tgagggggaa ctcgacgacg
    13621 gggactacac cgtttccgtt gccgaggaca cggttcgcga taccgccgag aacccgaacg
    13681 acgcagcgtc cctcgacttc agcgtc
//
LOCUS       DATMKC010000095         7666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_302815, whole genome shotgun sequence.
ACCESSION   DATMKC010000095 DATMKC010000000
VERSION     DATMKC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302815"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1279
                     /locus_tag="VNU01_02830"
     CDS             107..1279
                     /locus_tag="VNU01_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH31585.1"
                     /translation="MRARVSRWTTAALAALLALGALAPAGGQVPAEPDGPRIGTSFPE
                     DFPVIVDHSLGVPVIGFGAKGPVTRVPVVLLHGNNDTPYPTDCNGAYGKIQALAQHFA
                     DHGYAPSELWALGYQGEQCDLVTSPPNRSGPAHSTVENVPDLRAFVHAVLAYTGAGQV
                     DIVGHSLGATLPREWMRQDGAHHLVRRLVSIDGPHHGIINCSPNPRNIWQTPASGGFT
                     PDSAICVEYGADDTPFLRALNAGDETPGPTEYFAIRNADASFVFMDRQDGRVPPVPAE
                     DREGRPHDFSMSAALAGAQRSLDVTGAGAYDDALAAAHTGVVNSPEVWDAALAFLTEG
                     TEPPAQEVVDDGAPGGPASTGGPELPASGPAFPLALALAVLALGLALLPPPALRRR"
     gene            1358..2191
                     /locus_tag="VNU01_02835"
     CDS             1358..2191
                     /locus_tag="VNU01_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31586.1"
                     /translation="MSRALCLLIGAVLLVPVGTAAPAAGEGLQLPDLKPRSSTVAYNA
                     ATDELWFHTHVENHGEYAFEVLGSPHTDGPSQYAHADQCVRWADPRVHVGSKVCAEYQ
                     RIGRLVWHAEHRHLHLDGLARYTLFHVGEDGSLSEVRSSDKVGFCLTDTWPLRYERFP
                     QRVHPLLDPALDDQYGFRRTQTDRWYFECTYAYGVVPWPSLRMGISPGWKDIYNGPVP
                     GQQFPVGDLPDGQYRLRTRLNTSDSLRFREVSLDDNEHWLDVCLHRDAQGRRQAREGA
                     C"
     gene            complement(2192..3097)
                     /locus_tag="VNU01_02840"
     CDS             complement(2192..3097)
                     /locus_tag="VNU01_02840"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: protein motif:HMM:NF006186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HWH31587.1"
                     /translation="MGPVSEIFDASAWRPVEGFGFTDITYHRAVDAGVVRIAFDRPEV
                     RNAFRPHTVDELYTALDHARMSTDVGCVLLTGNGPSPRDGGWAFCSGGDQRIRGRDGY
                     RYAEGETADTVDPGRAGRLHILECQRLIRFMPKVVIAVVPGWAAGGGHSLHVVCDLTL
                     ASRQHARFKQTDADVGSFDGGYGSAYLARQVGQKFAREIFFLGREYDAAAAQRMGMVN
                     EVVDHADLERVALEWGREICGKSPTAQRMLKFSFNLIDDGLVGQQVFAGETTRLAYMT
                     DEAVEGRDAFLEKRAPDWSPFPYYY"
     gene            complement(3154..3921)
                     /locus_tag="VNU01_02845"
     CDS             complement(3154..3921)
                     /locus_tag="VNU01_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31588.1"
                     /translation="MHRFLRLTRAAAIAAALLLMLTACGDDDADSADGGDTVTAYCDY
                     VAELDDGESLPTEEQLDRLEELAPEEIAEEIAVASNGLREQGTNAFGDPDVNKAFDAI
                     ESYESEHCPDNGEDGEDGEEDEDEGPVAQEPDPDATRVDVVARDLEFVLPDGIAAGKT
                     AFVMTNEGEQPHHMSIVRFKADVTEEQVRATIDAGEDVQQHFESEVGESTDAAPGETA
                     VVNAELEPGLYGMACFVEGEDGVPHFFQGMYALFQVS"
     gene            complement(4022..4891)
                     /locus_tag="VNU01_02850"
     CDS             complement(4022..4891)
                     /locus_tag="VNU01_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31589.1"
                     /translation="MRTRAMAVVLAVALFGATAVLTVTMVFTCWDRSPCARGAVLAAL
                     AELALLGTAVSLAVALTGSTAWLWRRPARSVVGVLVVALLAMNAYGYWQESGQERREV
                     SGWLTGFRHGWVCYVDDGPDPHCMEGYVPGLQDIQPGDAFRGTIGRERRTGPWHLYLD
                     RAGPDVRPLVDRPGRPIPDAAVVRAQSVWTSWADARPTAQAPSRVAYVVEWTELAGPP
                     IGAAFRIAEPVPGLDLDVTLTCPSGRPATLWLGRGPASGVEALHEEAGWVRLRATVPA
                     ARIGEPFFCGPAA"
     gene            complement(4981..6126)
                     /locus_tag="VNU01_02855"
     CDS             complement(4981..6126)
                     /locus_tag="VNU01_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31590.1"
                     /translation="MRRTIFGPDHEAFRESAREYCDRHLRPNHERFIQQRGFDREIWL
                     EAGKQGFLGLEVAQEYGGSAAGDYRFNAVLTEELAKVGVSYASCFGIHFDIVAPYLVD
                     LCTDEQKRRWLPGFCSGEILTAIGMTEPSGGSDLAALRTTAVRDGGDWILNGSKTFIT
                     NGYTADLVVVAARTSPEKGAKGITLFGVEEGMKGFTRGRKLDKVGQHESDTAELFFED
                     VRVPDANRIGELDRGFIHMMERLPQERLGAAVTNIGQAADILAETVQYAKDRQAFGQP
                     IGKFQHNKFLLADLVTRVEVTQHYVDQCVAAHAGGELSAIDAAKAKWWSAEVQNDVLD
                     HCVQIFGGYGYMNEYRVARAWQDARVTKIWAGTNEIMKELIGRDLGL"
     gene            6575..7099
                     /locus_tag="VNU01_02860"
     CDS             6575..7099
                     /locus_tag="VNU01_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31591.1"
                     /translation="MGDSTILDGGGISCWAVDLERDYPLASALAATWDAGPARFLIRL
                     PDDVFGPTGVVGRVDLGRGEAVFDLPPPPGLAGLPYFDAAALLAAVLAEAPSWVYLGR
                     TEEGARFGRRDMAVAAALDRDWVVVAATIGGGGRLRSVEYLLPAQATLRPAVLRLFDF
                     DGAEPLDHAAAVPS"
     gene            7130..>7666
                     /locus_tag="VNU01_02865"
     CDS             7130..>7666
                     /locus_tag="VNU01_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31592.1"
                     /translation="MRARNIVVVLCVVWPLVLGPAAEASVPIIAGQSPLQRLAESRRT
                     ALRAAEALRGLGGTRLPVPRPNGRWLITFTDAETAAAAVDSPLLAGTGARGIARRVLL
                     LDRLPDGLTATELAAALPGALAVEADKSGYRAARVPSDPLVAEQWAHEQARAFEAWDT
                     TTGDAGVLVAVIDSGVWAE"
BASE COUNT         1023 a         2789 c         2772 g         1082 t
ORIGIN      
        1 caagggtacg gagccagagc ccgccacata cgaaaaaccg gtgggaaaga ctttggagaa
       61 tcgacaaggc ggttgcggcg tacaacggcg ggcaggaggt ggcggcatgc gcgcacgcgt
      121 gagcaggtgg acgacggcgg cactggcggc gctgctggcg ctgggcgcgc tggcccccgc
      181 gggcggccag gtgccggccg agccggacgg cccgcgcatc ggcacgtcgt tccccgagga
      241 cttcccggtg atcgtggacc actcgctggg ggtgcccgtg atcggcttcg gcgccaaggg
      301 gccggtgacg cgcgttcccg tcgtgctgtt gcatggcaac aacgacacgc cctacccgac
      361 ggactgcaac ggcgcctacg gcaagatcca ggcgctggcg caacacttcg ccgaccacgg
      421 ctacgcgccg agcgagctgt gggcgctggg ctaccagggc gagcagtgcg acctggtgac
      481 ctcgccgccg aaccgctccg gccccgcgca ctccacggtg gagaacgtgc cagacctgcg
      541 cgcgttcgtg catgccgtgc tggcctacac cggcgccggc caggtcgaca tcgtcggcca
      601 ctcgctgggc gcgacgctgc cgcgcgagtg gatgcgccag gacggcgccc accacctcgt
      661 gcggcggctg gtgtccatcg acgggccgca ccacggcatc atcaactgct cgccgaaccc
      721 gcggaacatc tggcagacgc cggccagcgg cggcttcacg cccgactcgg cgatctgcgt
      781 ggagtacggc gcggacgaca ccccgttcct gcgggcgctg aacgccgggg acgagacgcc
      841 ggggccgacc gagtacttcg cgatccgcaa cgccgacgcg agcttcgtgt tcatggaccg
      901 ccaggacggc cgtgtgccgc cggtgcccgc ggaggaccgc gagggccgcc cgcacgactt
      961 ctccatgagc gccgcgctgg cgggtgcgca gcggtcgctg gacgtcaccg gcgcgggcgc
     1021 ctacgacgac gcgctggccg ccgcgcacac cggcgtcgtg aacagccctg aggtctggga
     1081 cgcggcgctg gcgttcctga cggagggcac ggagccgccc gcgcaggagg tcgtcgacga
     1141 cggcgcgccc ggcggcccgg cgtccacggg cgggccggag ctgcccgcca gcggcccggc
     1201 gttccccctg gctctcgcct tggcggtgct ggcgctgggc ctcgccctcc tgccgccccc
     1261 cgcgctgcgc aggcgataac ggccaccatc ggccggaatc gactccgcag cggagggatc
     1321 ggcgttaggc atccggttat ccggacgtac cggtggcatg tcccgagccc tgtgtctcct
     1381 catcggcgcg gtgctgctcg tacccgtggg caccgccgct cccgccgccg gcgagggctt
     1441 gcagcttccc gacctgaagc cgcgctcgtc gaccgtcgcc tacaacgctg cgacggacga
     1501 gttgtggttc cacacccacg tcgagaacca cggcgagtac gccttcgaag tgctcggctc
     1561 gccgcacacc gacggcccgt cgcagtacgc ccacgccgac cagtgcgtgc gctgggccga
     1621 cccccgggtg cacgtcggct ccaaggtgtg cgcggagtac cagcgcatcg gtcggctggt
     1681 gtggcacgcc gagcaccgcc atctgcacct ggacggcctg gcgcggtaca cgctgttcca
     1741 cgtcggcgag gacggttcgc tgagcgaggt gcgcagctcc gacaaggtcg gcttctgcct
     1801 gaccgacacg tggccgctgc ggtacgagcg cttcccgcag cgcgtccacc cgctgctcga
     1861 cccggcgctg gacgaccagt acggcttccg gcgcacgcag accgaccgct ggtacttcga
     1921 gtgcacgtac gcctacggcg tggtgccgtg gccgagcctg cgcatgggca tctcaccggg
     1981 ctggaaggac atctacaacg gtcccgtgcc cggtcagcag ttcccggtcg gcgacctgcc
     2041 cgacggccag taccgcttgc gcacgcgcct gaacacctcg gacagcttgc gcttccgcga
     2101 ggtgagcctg gacgacaacg agcactggct ggacgtctgc ctgcaccggg acgcccaggg
     2161 ccggcgacag gcgcgcgagg gcgcctgctg atcagtagta gtaggggaag ggcgaccagt
     2221 ccggggcgcg cttctccagg aaggcgtcgc ggccctcgac cgcctcgtcc gtcatgtagg
     2281 caaggcgcgt ggtctcgccg gcgaacacct gctggccgac gagcccgtcg tcgatcaggt
     2341 tgaaggagaa cttgagcatg cgctgcgcgg tcgggctctt cccgcagatc tcacgacccc
     2401 actccagggc gacgcgctcc aggtcggcgt ggtccacgac ctcgttcacc atgcccatgc
     2461 gctgcgcggc cgcggcgtcg tactcgcgcc ccaggaagaa gatctcacgg gcgaacttct
     2521 gcccgacctg gcgcgccagg taggccgagc cgtagccgcc gtcgaaactg ccgacgtcgg
     2581 cgtcggtctg cttgaagcgc gcgtgctggc ggctggccag cgtgaggtcg cacaccacgt
     2641 gcaggctgtg cccgccgccg gccgcccagc ccgggaccac ggcgatgacg accttgggca
     2701 tgaagcggat caggcgctgg cactccagga tgtgcagccg tcccgcgcgg cccgggtcca
     2761 cggtgtcggc ggtctcgccc tcggcgtagc ggtagccgtc acggccgcgg atgcgctggt
     2821 ccccaccgga gcagaacgcc cagccgccgt ccctggggga agggccgttg ccggtcagca
     2881 gcacgcagcc gacatcggtg gacatgcgcg cgtggtcgag cgcggtgtag agctcgtcca
     2941 cggtgtgcgg gcggaaggcg ttgcgcacct cgggacggtc gaacgcgatg cgcaccacgc
     3001 ccgcgtcgac ggcgcgatgg taggtgatgt cggtgaagcc gaacccctcc acggggcgcc
     3061 acgccgacgc gtcgaagatc tccgagacag ggcccatggg gcgacaggct agcggctgcg
     3121 acgccgccgg cccgccgccc ccggcggtgc cgtctagctg acctggaaca gcgcgtacat
     3181 gccctggaag aagtgcggca cgccgtcctc gccttcgacg aagcaggcca tgccgtacag
     3241 gccgggctcg agctcggcgt tgaccaccgc cgtctcgccg ggagccgcgt ccgtgctctc
     3301 gcccacctcg gactcgaagt gctgctgcac gtcctcgccc gcgtcgatgg tcgcccgcac
     3361 ctgctcctcg gtgacgtcgg ccttgaagcg cacgatgctc atgtggtggg gctgctcgcc
     3421 ctcgttcgtc atcacgaacg cggtcttgcc ggcggcgatg ccgtcgggca gcacgaactc
     3481 gaggtccctg gcgacgacgt ccacccgggt ggcgtcgggg tcgggctcct gcgcgacggg
     3541 cccctcgtcc tcatcctctt cgccgtcctc gccgtcctcg ccgttgtccg ggcagtgctc
     3601 ggactcgtag gactcgatcg cgtcgaacgc cttgttgacg tccgggtcac cgaacgcgtt
     3661 ggtgccctgc tccctgaggc cgttcgacgc gacggcgatc tcctcggcga tctcctcggg
     3721 ggccagctcc tcgagccggt ccagttgctc ctcggtcggc aggctctccc cgtcgtccag
     3781 ttccgccacg tagtcgcagt acgcggtgac ggtgtcgcca ccgtccgcac tgtcggcgtc
     3841 gtcatcgccg cacgcggtga gcatgagcag cagcgcggcc gcaatggcgg cggcgcgggt
     3901 gagacgcaga aaacgatgca tcgaacattc ctcccgacca tgaaacgatt cgccgccggc
     3961 ggcggcggcg cagcatacgc gtccggagtg tccggatggc gtaagaatca gcgcgcctgc
     4021 ctcaggccgc ggggccgcag aagaacggct ccccgatgcg ggcggcgggc acggtcgcgc
     4081 gcagccgcac ccagcccgcc tcctcgtgca gcgcctcgac ccccgaggcg ggaccgcgtc
     4141 cgagccacaa ggtcgccggc ctgccggacg ggcaggtcag cgtgacgtcc aggtccaggc
     4201 caggcaccgg ctcggcgatg cggaacgccg ccccgatggg cgggcccgcc agctcggtcc
     4261 actccaccac gtaggcgacg cgggacggcg cctgggcggt cgggcgggca tcggcccacg
     4321 acgtccacac cgactgcgcg cggacgacgg cggcgtccgg gatggggcgg cccggccggt
     4381 ccaccagcgg gcgcacgtcc gggcctgcgc ggtccaggta caggtgccat ggccccgtcc
     4441 gccgctcacg gccgatagtg ccccggaatg cgtcgcccgg ctggatgtcc tgcaggcccg
     4501 gcacgtaacc ctccatgcag tgcgggtcgg gcccgtcgtc gacgtagcag acccagccgt
     4561 gacggaagcc ggtgagccag ccggagacct cgcggcgctc ctgcccggac tcctgccagt
     4621 agccgtaggc gttcatggcg agcagcgcga cgaccagcac gccgacgacc gagcgggccg
     4681 ggcgtcgcca cagccacgcg gtggagccgg tcagcgcaac cgccaggctc acggcggttc
     4741 cgagcagcgc gagctcggcc agtgcggcca ggaccgcgcc gcgcgcgcag gggctgcgat
     4801 cccagcaggt gaacaccatg gtgacggtga gcaccgccgt cgcgccgaac agggcgacgg
     4861 cgagcaccac ggccatcgcc cgcgtccgca tcagcaggat gatcggcgcc cggccccctc
     4921 accttcaccg tgcggagtcg gttccggccg atagtggcca gaatcgactc cgcagcggga
     4981 tcacaggccg aggtcgcggc cgatgagctc cttcatgatc tcgttggtgc cggcccagat
     5041 cttggtgacg cgcgcgtcct gccacgcgcg cgccacgcgg tactcgttca tgtagccgta
     5101 gccgccgaag atctgcacgc agtggtccag cacgtcgttc tgcacctcgg cgctccacca
     5161 cttggccttg gccgcgtcga tcgcgctgag ctcgccgccg gcgtgcgcgg cgacgcactg
     5221 gtcgacgtag tgctgggtca cctccacgcg cgtgaccagg tcggccagca ggaacttgtt
     5281 gtgctggaac ttgccgatgg gctggccgaa cgcctggcgg tccttggcgt actggacggt
     5341 ctcggccagg atgtccgcgg cctggccgat gttggtcacc gccgcgccga gccgctcctg
     5401 cggcagccgc tccatcatgt ggatgaagcc gcggtccagc tcgccgatgc ggttggcgtc
     5461 cgggacgcgc acgtcctcga agaacagctc ggcggtgtcg gactcgtgct ggccgacctt
     5521 gtccagcttg cgcccgcgcg tgaagccctt catgccttcc tcgacgccga acagcgtgat
     5581 gcccttggcg cccttctccg gcgacgtgcg cgccgcgacc acgacgaggt ctgcggtgta
     5641 gccgttggtg atgaacgtct tggagccgtt caggatccag tccccgccgt cgcgcaccgc
     5701 cgtggtccgc agcgcggcga ggtcgctgcc gccggacggc tcggtcatgc cgatggcggt
     5761 gaggatctcc ccggagcaga agccgggcag ccagcgccgc ttctgctcgt cggtgcacag
     5821 gtcgacgagg tagggcgcca cgatgtcgaa gtggatgccg aagcagctcg cgtagctgac
     5881 gccgaccttg gcgagctcct cggtcagcac ggcgttgaag cggtagtcgc ccgccgcgct
     5941 gccgccgtac tcctgggcca cctccaggcc caggaagccc tgcttgcccg cctccagcca
     6001 gatctcccgg tcgaagccgc gctgctggat gaagcgctcg tggttgggcc gcagatggcg
     6061 gtcgcagtac tcgcgcgccg actcgcggaa cgcctcgtgg tccggaccga agatcgtgcg
     6121 tcgcaccgct gtgcctttcc cgctgtgccg atgggccgat atcgccaatt gccgcatcgg
     6181 gttatacccc cctggtcggc ggcgtgaggc gggggacgat ggacagggac cgagaggagt
     6241 ggggcgagcc gcacggcccc atgccgtggc gtccgcgcac gggggcgctc ggttgcctgg
     6301 cgctcgtcct ggcactggtc ctggtcgcgt tcgcggtccc ggccgccatc gacagcggcc
     6361 tggaggacct gccgcccgag cgccgcgccg ccggcgccga ggcgctgatg ctggcgtcca
     6421 tcggctgccg cgacaacccg atcgggcggg tcctcgcccc cgcgctgaag ctcacctggc
     6481 tgagccgcgc ctgggacccg gcggccgaac cacaggcggg atggcgcccg gagatcgccg
     6541 cccggttgcg ctcctacacc tgttcggcct gccggtgggc gacagcacca tcctggacgg
     6601 cggcggcatc tcctgctggg cggtcgacct cgagcgggac tacccgctgg cctccgcgct
     6661 ggccgcgacg tgggacgccg ggccggcgcg cttcctgatc cggctgccgg acgacgtgtt
     6721 cgggccgacc ggcgtggtcg gccgcgtcga cctgggccgc ggagaggcgg tcttcgacct
     6781 gccgccgccg cccggtctcg ccgggctgcc ctacttcgac gccgccgcgc tgttggcggc
     6841 ggtcttggcg gaggcgccga gctgggtcta cctcggcagg acggaggagg gcgcgcgctt
     6901 cggccgccgc gacatggccg tggcggccgc gctggaccgc gactgggtgg tggtcgccgc
     6961 caccatcggc ggcggcggcc ggctgcgcag cgtcgagtac ctgctgccgg cgcaggccac
     7021 gctgcggccc gccgtgctgc gcctgttcga cttcgacgga gcggagcctt tggaccacgc
     7081 ggccgcagta ccctcgtgac ggtcgggagc gcggcagaaa ggcgaaggcg tgcgggcgcg
     7141 gaacatcgtg gtggtgctgt gcgtggtgtg gcccctggtc ctggggccgg cggcggaggc
     7201 gtccgtgccg atcatcgccg ggcagtcacc cctgcagcgc ctggcggagt cgcggcgcac
     7261 ggcgctgcgc gccgcggagg cccttcgcgg cctcggcggc acccgcttgc ccgtgccgcg
     7321 ccccaacggg cgctggctga tcacgttcac cgacgccgag acggccgcgg cggccgtcga
     7381 ctcgcccctc ctggcgggga ccggggcgcg cggcatcgcg cggcgcgtcc tgctgctgga
     7441 ccgcctgccg gacggcctga cggccaccga gttggcggca gcgctgcccg gcgccctggc
     7501 cgtggaggcc gacaagtccg ggtaccgcgc cgcgcgcgtg ccgtccgacc cgctggtcgc
     7561 cgagcagtgg gcccacgagc aggcgcgcgc gttcgaggcg tgggacacga ccacgggcga
     7621 cgccggcgtg ctggtggccg tgatcgacag cggcgtgtgg gcggaa
//
LOCUS       DATMKC010000096        11562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_302725, whole genome shotgun sequence.
ACCESSION   DATMKC010000096 DATMKC010000000
VERSION     DATMKC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302725"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VNU01_02870"
     CDS             complement(<1..628)
                     /locus_tag="VNU01_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31593.1"
                     /translation="MQQPVDYQLADTADLARGWLSEHAAGAGIVAVDTETTGWDPLRD
                     RLLLVQVSAGPDRPVLVLDAQRVDPLVLQPLFADEQALKVFHHGAFDLRFLSMAGLRV
                     RRVADTMLAQQLLDGGESTEAGLGLAGLADFRLGMRLDKTVRDTFQDPGQADPTEQQL
                     AYAAADAAATWGVFDQQWRELVGHGLTRVAQLEFAALPVLAAMQLRGVA"
     gene            complement(568..2091)
                     /locus_tag="VNU01_02875"
     CDS             complement(568..2091)
                     /locus_tag="VNU01_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWH31594.1"
                     /translation="MTVHIPSRLRVIGVTVLVACLTLSMAGESLAATSLDKARARRAE
                     LEQRIAQVSQQLEDVAARVDEAETLRHTLETDVAALQERATHADRALHARAVALYKRG
                     DMGVLQQLITAGGPAEVVQRSRLLAGLGRREQETIERAAVARLTLARRKAELEQVTQR
                     LARDQRHLAKLRAELGRAFADAKAREGELVSRRGRQRKVSRARQNGVYACPLGRPFHF
                     RDTWGAPRSGGRRHKGVDMFAPMNTPVYAMTNGVISRQSSSRLGGLGLYLRGDDGNVY
                     YYAHLRRIHPGYGPGRRVEAGELIAYNGDSGNARGGAPHVHFEVHPGGDRAAGEDGAD
                     LAHRHDVGVHRALHGQGVAGLCDRRAVRPPQHHGVGREVHGVALRPLHLVALAVGLHP
                     RAAALQPGVRAEGAGDGRQLVVVRLRAGRVPAVSRLVEDRLVRGRGGVVATGGQQQRG
                     GEQERERSHRWSLSRMHQVGRSDARRRRFVPSRADWGRCSSPSTISSPTPPTSRAAG"
     gene            2279..4285
                     /locus_tag="VNU01_02880"
     CDS             2279..4285
                     /locus_tag="VNU01_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HWH31595.1"
                     /translation="MRFPDPRGTSEPMREKDRPWLMRTYSGHSSARASNELYRTNLAK
                     GQTGLSVAFDLPTQTGYDADHVLARGEVGKVGVPICSIDDMRTLFDGIPLGEMNTSMT
                     INATAAWLLALYVAAAEEQGAPRAALQGTTQNDIVKEYLSRGTYVFPPQPSMRLTADV
                     ITYTVREIPKWNPMNVCSYHLQEVGATPVQEVAYAMANATAILDAVRASGAVPEAEFG
                     QVVGRISFFVNAGIRFVEEIAKLRVMADLWDGLCRERYGVTDEKLRRFRYGVQVNSLG
                     LTEAQPENNIARILLEMLAVTMSKKARARAIQLPAWNEALGLPRPWDQQLSLRMQQIL
                     AYETDLLEYGDLFDGSPVMAGIEAEIAEGAREEYRKVLEMGGAVEAVEYMKTELVRSN
                     AERIRKVESGERTVVGVNKFVETEPSPLVSEEDAGILTVDPRAEAEQIERLEAFRATR
                     DNSAVAKALDALKRAAESGENIMPASIEAAHAGVTTGEWSDALRQAFGEYRGPTGVSG
                     QTAAIDMAQLEQARAKVAEAEKAVGGKIKLLVGKPGLDGHSSGAEQIAVRARDAGFEV
                     IYQGIRLTPAQIVRAAADEDVHVVGLSILSGSHAELVPQVVDGLREQGLDVPVVVGGI
                     IPPADARMLRERGVAAVYTPKDYEVTRIIGEIADLVASRAAQPA"
     gene            complement(4341..5099)
                     /locus_tag="VNU01_02885"
     CDS             complement(4341..5099)
                     /locus_tag="VNU01_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWH31596.1"
                     /translation="MCHEPDDHPPEAPAQGMLAGVQRRTLTASDGAECAATVATTRRQ
                     GAPGIVIVPDVRGLHPFYERLAEQYAQAGVHALAVDQYGRTAGAEWRGAGFAYGPLVA
                     DASATVHLDIAAAAQALREAGCERVFVTGFCFGGRAALMAAAAEGVDGVIGFYGWPVT
                     AGVDGASPESLARERRLRAPVLALFGEADEGIPPGDVERYADALRASGVAHDVRVYAD
                     APHSFFDRARTGHAGHCADAWGRALAFIETGHPA"
     gene            complement(5136..5543)
                     /locus_tag="VNU01_02890"
     CDS             complement(5136..5543)
                     /locus_tag="VNU01_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31597.1"
                     /translation="MAGVVEAVSASPRHTVAKRNQLWIRLSEGLGVEGDAHAGRTVRH
                     RSRVAREHTQPNLRQVHLIHAELLDELRAAGFAVSPGRMGENVTTRGVDLLGLPRGAL
                     LRLGAEAVVELTGLRDAVGVELPAGPHERLVPV"
     gene            complement(5553..6773)
                     /gene="ilvA"
                     /locus_tag="VNU01_02895"
     CDS             complement(5553..6773)
                     /gene="ilvA"
                     /locus_tag="VNU01_02895"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWH31598.1"
                     /translation="MSASGSDLVSLDDVRAAARVLDEVAVRTPVEESRALSAAAGARV
                     LLKCEHLQRTGSFKIRGAYYRISRLTDDERARGVACASAGNHAQGVALSARLAGVRAT
                     VFMPEGAPLPKVEATRAYGAEVVLTPGGFDDSASACAAWAEREGAVVVPPFEHPDIVA
                     GQGTVGLEIAEQVPDAGSVVVAVGGGGLAAGVATAMKGLRPGVQVVGVQAEAVAPAAA
                     LLAGRPAQDGVRGHTIADGIAVKQPGALTMAHLRARLDGIVTVPDESIARAVLLLAER
                     AKQVVEPSGAAPVAALLDGALAGLPGPLVLVLGGGNVDPLLLLRILRAGMGEEGRTWS
                     FRTRLPDQPGALSAMLALIAREGANVVAVEHHRYGTRFELDEVEVYLELETRGGEHIA
                     ELREALRADGYALD"
     gene            complement(6816..7124)
                     /locus_tag="VNU01_02900"
     CDS             complement(6816..7124)
                     /locus_tag="VNU01_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31599.1"
                     /translation="MRGKGERLAIRIRGGRGGVGVAAGAAVATLAVGMLAAGLMLQRF
                     FDPEVMGALSVLVWPAWLLGAMLTFFGLVAFAIAVVSTAAGVGLSALRGAPAQGSAGS
                     "
     gene            complement(7227..7964)
                     /locus_tag="VNU01_02905"
     CDS             complement(7227..7964)
                     /locus_tag="VNU01_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HWH31600.1"
                     /translation="MASSPAEPREGERRALERLTAARLEPLGWIRAASNEALLVRLDG
                     SPDDLAVYKPDSGERPLWDFPPATLARRETAAFALSDWLGWDVVPATVYRDGPHGPGS
                     VQRFVPHDPARHYFVLLEDESLHPVLRRVALFDLVTNNADRKASHVMLGPEGVRGCDH
                     GLCFHVEPKLRTVIWDFAGEPIGVEDRDDLRRLADALDSGGGPALGGLLDPAECAALL
                     RRTRALLGLGHLPDPPEDRRPYPWPLF"
     gene            complement(7945..8490)
                     /locus_tag="VNU01_02910"
     CDS             complement(7945..8490)
                     /locus_tag="VNU01_02910"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HWH31601.1"
                     /translation="MGESVEFESTEWITAGAVGEPGARTFFIQARAEGRVVALLVEKG
                     QVAGIAELAQELLGRFDVTVTPDDLDEAHHRLIDGIEPLWRVGELSLGASSDGERFVL
                     EAMEILFIDPDEAPDDVLAEEPGQARFWLTRHQLVRLAAYAAYAVEAGARERCRLCGR
                     PIDPVEGHVCPATNGHGKLTR"
     gene            complement(8483..9145)
                     /locus_tag="VNU01_02915"
     CDS             complement(8483..9145)
                     /locus_tag="VNU01_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWH31602.1"
                     /translation="MSTLLLVRHATTAATGKKLGGRTEAPLDDAGRAQASAAAGRLRG
                     LKVTAVYASPLARTMETARIVAAPHGLGVRPAEGLLEVEYGRWTDRPLAQVARTKLWP
                     VIQQRPSQVTFPDGESIRAMQARAVDAVEEIVARHPKGTVVAVSHADVIKALVSFYLG
                     QPLDLFQRIHIAPASVTALQLSPGGTPVLTRLNDVGPPGGGPGDDGPWPGAEKGKAAR
                     HG"
     gene            9228..10625
                     /locus_tag="VNU01_02920"
     CDS             9228..10625
                     /locus_tag="VNU01_02920"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWH31603.1"
                     /translation="MADTRIERDSMGEMEVPASAYYGAQTQRAVENFPISGQPIPREL
                     IRALGLIKASAAVVNHAKGVLSDEQFAAIAQAAQEVADGELDAHFPVDVFQTGSGTSS
                     NMNANEVIANRATEILGGELGSKLVHPNDHVNAGQSSNDVFPTAVHVAAYTASVERLL
                     PALETLRASLAAKADEFADVVKPGRTHLMDATPVTLGQEFSGYARQIELGAARVRAAL
                     ERVAELPLGGTAVGTGLNAPEGMTEGVIAELRARTGIAELREADNHFEAQGARDALVE
                     LSGAFKVIAVSLTKIANDVRWMGSGPRTGFGEIQLPEVQPGSSIMPAKVNPVIPESVT
                     MVAAQVIGNDGAVTVGGLSGAFELNVYIPMMARNVLESGRLLATTCANFAAQCVDGIT
                     ANVERCWELSERNLSTVTALVPAIGYDKSAELAKKALKEDRPLREVVKEAGVLPEADV
                     DRILDTKKMTQGGVL"
     gene            10746..>11562
                     /locus_tag="VNU01_02925"
     CDS             10746..>11562
                     /locus_tag="VNU01_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31604.1"
                     /translation="MGRRWSAVLLVGFGLAALPASAVTAPALPAAPPVTVHEGAPHTP
                     AAPVSSAAATDLRGTFRITAGACAGAVTGSRFRMVQPGGTPASGPFVENADSPCADKT
                     YTPLRPGTDGGLVSGSHQPQPSAPFDGTGNGTAARIVRPQRFFGVDFAVATNPTDPQS
                     GTDTPAPVIRRDGSTLSGDLRAYAVAWNRQHFNQGAPKPDGSTPGQTTLPSGTIDAST
                     KAYRLEWASTIVGGPFNNFTGLWHLEGTFDGALGDQAGPVSQPPGSVPAPPAGG"
BASE COUNT         1608 a         4358 c         4195 g         1401 t
ORIGIN      
        1 acgccacgcc gcgcagctgc atcgccgcca ggacgggcag cgccgcgaac tccagctggg
       61 ccacgcgcgt gagcccgtgc ccgacgagct cccgccactg ctggtcgaac acgccccagg
      121 tcgcggcggc gtccgcggcc gcgtaggcga gctgctgctc ggtcgggtcg gcctggccgg
      181 ggtcctggaa cgtgtcgcgc accgtcttgt ccaggcgcat gcccaggcgg aagtcggcca
      241 ggccggccag tcccaggccc gcctccgtgg actcgccgcc gtccagcagc tgctgggcca
      301 gcatcgtgtc cgcgacgcgg cgcacgcgca gcccggccat ggacaggaag cgcaggtcga
      361 acgcgccgtg gtggaacacc ttgagcgcct gctcgtcggc gaacagcggc tggagcacca
      421 gcgggtcgac gcgctgggcg tccagcacca gcacgggacg gtccggcccc gcggacacct
      481 ggacgagcag cagccggtcg cgcagcgggt cccagcctgt ggtctcggtg tccaccgcga
      541 cgatgcccgc gccggccgcg tgctcgctca accagccgcg cgcgaggtcg gcggtgtcgg
      601 cgagctgata gtcgacgggc tgctgcatcg gccccagtct gcacgcgagg gaacgaaacg
      661 gcgccgccgt gcgtcggacc tgccgacctg atgcattcga gaaaggctcc accgatgcga
      721 ccgctcgcgc tcctgttcgc cgccacgctg ctgctgaccg cctgtggcga cgacgccgcc
      781 tctcccccgg acgagccggt cctcgaccag ccggctgacg gcggggaccc ggccggcacg
      841 cagccgcacg acgacgagct ggcgtccatc accggcacct tcggcgcgga cgccgggctg
      901 gagggcggct gcgcgtgggt ggagaccgac ggccagcgct acgaggtgca gtggccggag
      961 ggctacaccg tggacttcgc ggccgacccc gtggtgctgc gggggccgga cggcgcggcg
     1021 gtcgcacagg ccggcgacac cctgaccgtg gagggctcgg tgaacaccga cgtcatgacg
     1081 gtgtgccaga tcggccccgt cttcaccggc agcgcggtcg ccgcccggat gcacctcgaa
     1141 gtgcacgtgc ggcgcgccgc cgcgggcgtt gccggagtcg ccgttgtagg cgatcagctc
     1201 gcccgcctcg acgcgccgtc ccgggccgta gccggggtgg atgcgccgca ggtgcgcgta
     1261 gtagtagacg ttgccgtcgt cgccgcgcag gtacagcccc aggccgccca ggcgggagct
     1321 ggactgccgg ctgatcacgc cgttggtcat ggcgtacacc ggcgtgttca tgggggcgaa
     1381 catgtccacg cccttgtggc ggcgcccgcc ggagcgcggc gccccccacg tgtcgcggaa
     1441 gtggaacggc cggcccagcg ggcaggcgta cacgccgttc tgccgggccc gggacacctt
     1501 gcgctggcgg ccccggcggc tgaccagctc gccctcgcgc gccttggcgt cggcgaaggc
     1561 ccggcccagc tcggcgcgca gcttggccag gtgccgctgg tcgcgcgcca ggcgctgcgt
     1621 gacctgctcc agctcggcct tgcgccgggc cagcgtgagc cgggccaccg cggcccgttc
     1681 gatcgtctcc tgctcccgtc ggcccaggcc ggccagcagg cgcgagcgct gcaccacctc
     1741 ggcggggccg cccgcggtga tgagctgctg cagcacgccc atgtccccgc gcttgtacag
     1801 cgcgacggcg cgggcgtgca gggcgcgatc ggcgtgggtg gcgcgctcct ggagcgcggc
     1861 cacgtccgtc tcgagcgtgt ggcgcagcgt ctccgcctcg tccacgcgcg cggccacgtc
     1921 ctccagctgc tggctgacct gggcgatgcg ctgctccagc tccgcgcgcc gcgcccgcgc
     1981 cttgtccagc gacgtcgccg ccagcgactc gccggccatg gacagggtga gacaggccac
     2041 caggacggtg accccgatca cacgcaggcg ggacgggatg tggacggtca tctcgctttc
     2101 ggcgggcgcg cgtgggcaag ccgccgggga agggaatcgt caagagcgta ctcacccgcc
     2161 gaaacggacg gcaaggcgcc cacgagcccg gagagcccgg cgaatacgtt gaaatcgggc
     2221 atgtcgccct ggcccgttgg acgggtcggc gggcggcgag cgacaatccc ccggcaccgt
     2281 gcgcttcccc gacccgagag gaacgagcga accgatgcgc gagaaggatc gaccctggct
     2341 gatgcggacc tactccgggc actcctccgc ccgggcgtcc aacgagctgt accgcaccaa
     2401 cctcgccaag ggccagacgg gcctgtcggt cgccttcgac ctgccgacgc agaccggcta
     2461 cgacgccgac cacgtgctgg cgcgcgggga ggtcggcaag gtcggcgtgc cgatctgctc
     2521 catcgacgac atgcgcacgc tgttcgacgg catccccctg ggcgagatga acacctccat
     2581 gacgatcaac gcgacggccg cgtggctgct ggcgctgtac gtcgccgccg ccgaggagca
     2641 gggcgccccg cgcgcggcgc tgcagggcac cacgcagaac gacatcgtca aggagtacct
     2701 gtcgcgcggc acctacgtgt tcccgccgca gccgtccatg cggctgaccg ccgacgtcat
     2761 cacctacacc gttcgcgaga tccccaagtg gaacccgatg aacgtgtgca gctaccactt
     2821 gcaggaggtc ggcgcgacgc cggtgcagga ggtcgcctac gcgatggcca acgccaccgc
     2881 gatcctcgac gcggtgcgcg cctccggcgc ggtgcccgag gccgagttcg gccaggtggt
     2941 cgggcgcatc agcttcttcg tgaacgccgg catccgcttc gtggaggaga tcgcgaagct
     3001 gcgcgtcatg gcggacctgt gggacgggct gtgccgcgag cgctacggcg tgaccgacga
     3061 gaagctgcgc cgcttccgct acggcgtgca ggtcaactcc ctgggcctga ccgaggcgca
     3121 gccggagaac aacatcgcgc gcatcctgct ggagatgctg gccgtcacca tgagcaagaa
     3181 ggcgcgcgcc cgcgcgatcc agctcccggc gtggaacgag gcgctgggcc tgccgcggcc
     3241 gtgggaccag cagctcagcc tgcgcatgca gcagatcctg gcctacgaga cggacctgct
     3301 ggagtacggc gacctgttcg acggctcgcc ggtcatggcc ggcatcgagg ccgagatcgc
     3361 cgagggcgcg cgcgaggagt accgcaaggt gctggaaatg ggcggcgccg tggaggccgt
     3421 ggagtacatg aagaccgagc tggtgcgcag caacgccgag cgcatccgca aggtcgagtc
     3481 cggcgagcgc acggtcgtcg gggtcaacaa gttcgtcgag accgagccgt cgccgctggt
     3541 gagcgaggag gacgcgggca tcctcacggt ggacccgcgc gccgaggccg agcagatcga
     3601 gcgcctggag gcgttccgcg cgacacgcga caactccgcg gtcgccaagg ccctcgacgc
     3661 gctgaagcgc gcggccgagt ccggcgagaa catcatgccg gcgtccatcg aggcggccca
     3721 cgccggcgtg accaccggtg agtggtccga cgccctgcgc caggcgttcg gcgagtaccg
     3781 cggcccgacc ggcgtgtccg ggcagacggc ggccatcgac atggcgcagc tggagcaggc
     3841 gcgcgccaag gtggcggagg ccgagaaggc cgtgggcggc aagatcaagc tgctcgtggg
     3901 caagcccggc ctggacggtc acagctccgg cgccgagcag atcgcggtgc gcgcgcgcga
     3961 cgcgggcttc gaggtgatct accagggcat ccgcctcacg ccggcccaga tcgtgcgcgc
     4021 cgcggccgac gaggacgtgc acgtcgtggg cctgtcgatc ctgtccggct cgcacgccga
     4081 gctggtgccg caggtggtgg acggcctgcg cgagcagggc ctggacgtgc ccgtggtggt
     4141 cggcggcatc atcccgccgg ccgacgcgag gatgctgcgc gagcgcggcg tcgcggccgt
     4201 ctacacgccc aaggactacg aggtgacgcg catcatcggc gagatcgccg acctggtcgc
     4261 ctcccgcgcc gcccagcccg cctgaccggg gtgtgtcgag taagtcaacg ccacgtgtac
     4321 tgagtcgaca caccccgggt tcaggcgggg tggccggtct cgatgaaggc cagggcgcgg
     4381 ccccacgcgt cggcgcagtg cccggcgtgc cccgtgcgcg cccggtcgaa gaagctgtgc
     4441 ggcgcgtccg cgtagacgcg gacgtcgtgc gccacgccgc tggcccgcag cgcgtcggcg
     4501 tagcgctcga cgtcgcccgg cgggatgccc tcgtcggcct cgccgaacag cgcgagcacc
     4561 ggggcgcgca gccgccgctc ccgcgccagc gactccggtg acgcgccgtc cacgccggcc
     4621 gtcaccggcc agccgtagaa gccgatgacc ccgtccaccc cttcggccgc cgcggccatc
     4681 agcgccgcgc ggccgccgaa gcagaacccg gtgacgaaca cccgctcgca gcctgcctcg
     4741 cgcagcgcct gcgccgccgc ggcgatgtcg aggtggaccg tggccgaggc gtcggcgacc
     4801 agcggtccgt acgcgaagcc ggcgccgcgc cactcggcac cggcggtgcg gccgtactgg
     4861 tccaccgcca gggcgtggac gcccgcttgg gcgtactgct cggccagccg ttcgtagaac
     4921 gggtgcagcc cgcgcacgtc cggcacgatc acgatgccgg gagcgccctg gcgccgcgtc
     4981 gtggcgacgg tcgccgcgca ctcggcgcca tcgctcgcgg tcagtgtccg ccgttgcacg
     5041 ccggcgagca tgccctgcgc gggcgcctcg ggcggatggt cgtcaggctc gtggcacacg
     5101 gcatgcctcc acggtcgggg cggtcattcg accactcaca ccggcaccag gcgctcatgc
     5161 ggcccggcgg gcagctcgac gcccacggcg tcgcgcaacc cggtgagctc gaccaccgcc
     5221 tcggcaccga gccgcagcag ggcaccgcgc ggcaggccga gcaggtcgac gccgcgcgtg
     5281 gtgacgttct cgcccatgcg ccccggcgag accgcgaagc ccgcggcgcg cagctcgtcc
     5341 agcagctcgg cgtggatgag gtggacctgg cggaggttgg gctgggtgtg ttcgcgcgcg
     5401 acgcgcgagc ggtggcgcac cgtgcgaccg gcgtgggcgt cgccctcgac gccgagcccc
     5461 tcactcaggc ggatccagag ctggttgcgc ttggcgacgg tgtggcgcgg gctcgcgctc
     5521 accgcctcca ccacgcccgc catcgctccc cctcagtcca gcgcgtagcc gtcggcccgc
     5581 agcgcctcgc ggagctcggc gatgtgctcg ccgccgcgcg tctccagctc cagatacacc
     5641 tcgacctcgt ccagctcgaa acgcgtgccg tagcgatggt gttccacggc cacgacgttg
     5701 gccccctcgc gcgcgatcag cgccagcatc gccgacaggg cgcccggctg gtccggcagg
     5761 cgcgtgcgga acgaccacgt ccggccctcc tcgcccatgc cggcgcgcag gatccgcagc
     5821 agcagcagcg ggtcgacgtt gccgccaccc agcaccagga ccagcggccc gggcaggccg
     5881 gccagggcgc cgtcgagcag cgcggcgacc ggtgcggcgc cgctgggctc caccacctgc
     5941 ttggcccgct cggccagcag cagcacggcg cgcgcgatgg actcgtccgg cacggtgacg
     6001 atgccgtcca ggcgcgcgcg caggtgcgcc atcgtgagcg cgcccggctg cttgaccgcg
     6061 atgccgtcgg cgatggtgtg gccgcgcacg ccgtcttgcg ctgggcggcc cgcaagcagc
     6121 gcggcggccg gcgcgaccgc ctccgcctgc acgccgacga cctgcacgcc ggggcgcagc
     6181 cccttcatcg cggtcgccac tccggcggcc aggccgccgc cgcccacggc gaccaccacg
     6241 ctgccggcgt cgggcacctg ctcggcgatc tccaggccca cggtgccctg gccggccacg
     6301 atgtcggggt gctcgaacgg cggcaccacc acggcgccct cgcgctcggc ccatgcggcg
     6361 caggccgacg cgctgtcgtc gaagccgccc ggcgtcagca ccacctcggc gccgtacgcg
     6421 cgcgtggcct ccaccttggg cagcggcgcc ccctccggca tgaacaccgt ggcgcgcacg
     6481 ccggccaggc gggccgacag cgccacaccc tgcgcgtggt tgcccgccga ggcgcacgcc
     6541 acgccgcgcg ctcgctcgtc gtcggtcagg cgcgagatgc ggtagtaggc gccgcggatc
     6601 ttgaacgagc cggtgcgctg gaggtgctcg cacttgagca gcacgcgcgc gcccgccgcg
     6661 gcggacagcg cgcgggactc ctcgacgggc gtgcgcacgg ccacctcgtc caggacgcgc
     6721 gcggcggcgc gcacgtcgtc gaggctgacg aggtcggagc cggaggcgga catgccctca
     6781 ggctacggct cccgaggcgt cctcggcgcc tgcggtcagg agccggcgga gccttgcgcg
     6841 ggagcgccac gcagggcgct caggcccacg cccgcggccg tggacaccac ggcgatcgcg
     6901 aaggcgacca gtccgaagaa cgtcagcatg gcgccgagca gccaggccgg ccacaccagc
     6961 acggacagcg cgcccatcac ctcggggtcg aagaagcgct gcagcatgag ccccgcggcg
     7021 agcatgccga cagcgagcgt cgccacggcc gccccggcgg ccacgccgac gccgccgcgc
     7081 ccgccgcgga tccggatcgc gagccgttca cccttgccgc gcatggcgtt cctccacctc
     7141 gatggtgcgc tcgtggggga agcatcggca ccggcggacg cggcgcccat ccgccgatcg
     7201 ggtgacgccg tcccaacctg ggacggttag aagaggggcc aggggtacgg gcggcggtcc
     7261 tccggcgggt cgggcagatg cccgaggccc agcagcgcgc gcgtgcgccg cagcagcgcc
     7321 gcgcactccg ccgggtccag caggccgccc agcgccggcc cgccgccgga atcgagcgcg
     7381 tcggccaggc gccgcagatc gtcccggtcc tccacgccga tcggctcgcc cgcgaagtcc
     7441 cagatcacgg tgcgcagctt cggctcgacg tggaagcaca ggccgtggtc gcagccgcgc
     7501 acgccctccg gccccagcat gacgtgactg gccttgcggt cggcgttgtt cgtcaccagg
     7561 tcgaacagcg ccacgcgccg cagcacgggg tgcagcgact cgtcctccag cagcacgaag
     7621 tagtgccggg ccgggtcgtg cggcacgaag cgctgcaccg agccggggcc gtgcggcccg
     7681 tcgcggtaca cggtcgccgg caccacgtcc caccccagcc agtcgctcag cgcgaacgcg
     7741 gccgtctcgc gccgcgccag cgtcgcgggc ggaaagtccc acagcggccg ctcgccgctg
     7801 tcgggcttgt agacggccag gtcatccggg gagccgtcaa ggcgcaccag cagcgcctcg
     7861 ttgctcgccg cgcggatcca gcccagcggc tccagccgcg cggccgtcag gcgctccagc
     7921 gcccgccgct cgccctcccg cggctcagcg ggtgagcttg ccatgcccgt tggtcgccgg
     7981 gcagacgtgg ccctcgaccg ggtcgatggg ccggccgcac agccggcagc gctcacgggc
     8041 cccggcctcc acggcatagg cggcgtacgc ggccaggcgc accagctggt ggcgcgtcag
     8101 ccagaagcgc gcctgccccg gctcctccgc cagcacgtcg tcgggcgcct cgtccgggtc
     8161 gatgaacagg atctccatcg cctccagcac gaagcgctcg ccgtcggagc tcgcgccgag
     8221 gctcagctcc cccacccgcc acaagggctc gatgccgtcg atcaggcggt ggtgcgcctc
     8281 gtcgaggtcg tccggcgtga cggtgacgtc gaagcggccg agcagctctt gcgccagctc
     8341 ggcgatgccg gccacctgac ctttctccac cagcagggcg acgacgcggc cctcggcgcg
     8401 cgcctggatg aagaaggtgc gcgcgccggg ctcgcccacc gcgcccgcgg tgatccactc
     8461 ggtggactcg aactcgaccg actcacccat gccgggcagc cttccccttc tcggcccccg
     8521 gccatggccc gtcgtccccc gggccgccgc caggcggccc gacatcattg agccgggtca
     8581 ggacgggcgt gccgcccggc gacagctgga gcgcggtcac ggacgccggc gcgatgtgga
     8641 tgcgctggaa caggtccagc ggctggccca ggtagaagct gaccagcgcc ttgatcacgt
     8701 cggcgtggct cacggcgacc acggtgccct tggggtggcg cgcgacgatc tcctccaccg
     8761 cgtccaccgc gcgcgcctgc atcgcccgga tgctctcgcc atccgggaag gtgacctgag
     8821 acggccgctg ctggatgacc ggccacagct tggtcctggc cacctgcgcc aaggggcggt
     8881 cggtccagcg gccgtactcg acttccagca gcccctcggc ggggcgcacc cccagcccgt
     8941 gcggcgccgc gacgatgcgg gccgtctcca tcgtgcgggc gagcgggctc gcgtagaccg
     9001 ccgtgacctt gaggccccgc aggcgcccgg cggcggccga ggcctgcgcc cgaccggcgt
     9061 cgtcgagcgg cgcctccgtg cgcccgccca gcttcttgcc ggtggcggcg gtggtggcgt
     9121 ggcgcaccag cagcagggtc gacatgcggc gagcctatac cgctcgatac gctgcgcctc
     9181 cccgacccac gacagtggca accgacagcg aagggaacac gcaccccatg gccgacacgc
     9241 ggatcgaacg cgactcgatg ggcgagatgg aggtcccggc ctccgcgtac tacggtgcgc
     9301 agacgcagcg cgcggtggag aacttcccga tctccggcca gccgatcccg cgcgagctga
     9361 tccgcgcgct gggcctgatc aaggccagcg ccgcggtggt caaccacgcc aagggcgtgc
     9421 tgtccgacga gcagttcgcc gcgatcgccc aggccgcgca ggaggtggcc gacggcgagc
     9481 tggacgccca cttccccgtg gacgtgttcc agaccgggtc cggcaccagc tccaacatga
     9541 acgccaacga ggtcatcgcc aaccgcgcca ccgagatcct gggcggcgag ctgggctcca
     9601 agctggtgca ccccaacgac cacgtgaacg caggccagtc ctccaacgac gtgttcccga
     9661 cggccgtgca cgtcgccgcc tacaccgcct ccgtggagcg actgctgccg gcgttggaga
     9721 cgctgcgcgc gtcgctggcc gccaaggcgg acgagttcgc cgacgtggtc aagccggggc
     9781 gcacgcacct catggacgcc acacccgtga cgctgggcca ggagttctcc ggctacgcgc
     9841 gtcagatcga gctcggcgcc gcccgcgtgc gcgccgccct ggagcgggtg gccgagctgc
     9901 cgctgggcgg caccgcagtg ggcacgggcc tgaacgcgcc cgagggcatg accgaggggg
     9961 tcatcgccga gctgcgcgcc cgcaccggca tcgccgagct gcgcgaagcc gacaaccact
    10021 tcgaggcgca gggcgcgcgc gacgcgctgg tggagctgag cggcgcgttc aaggtcatcg
    10081 ccgtgtcgct caccaagatc gccaacgacg tccgctggat gggctccggc ccgcgcaccg
    10141 gcttcgggga gatccagctc cccgaggtgc agcccggcag ctcgatcatg cctgcgaagg
    10201 tcaaccccgt gatcccggag tcggtgacca tggtggccgc gcaggtgatc ggcaacgacg
    10261 gcgcggtgac ggtcggcggg ctgtccggcg ccttcgagct gaacgtctac atcccgatga
    10321 tggcgcgcaa cgtgctggag tccgggcggc tgcttgccac cacgtgcgcc aacttcgccg
    10381 cccagtgtgt cgacggcatc acggccaacg tcgagcgctg ctgggagctg tcggagcgca
    10441 acctgtcgac ggtgacggcg ctggtgccgg cgatcggcta cgacaagtcg gccgagctgg
    10501 ccaagaaggc gctgaaggag gaccgcccgc tgcgcgaggt ggtcaaggag gccggggtcc
    10561 tgccggaggc ggacgtggac cgcatcctcg acaccaagaa gatgacccaa ggcggcgtgc
    10621 tgtagcacgc cgccggtgaa aagtgctggg cggcgtgctc taggcttttc cacggaacga
    10681 acacggaggc gggtgcggtt cggcgcatat acgcgccggg gtgcacccac ctcgggggtg
    10741 atcttgtggg cagacggtgg agtgcggtcc tgctggtggg cttcggcctg gcggcccttc
    10801 cggcgtcggc ggtaaccgcg ccggcgctgc cggcggcgcc gccggtcacc gtccacgagg
    10861 gcgcgcccca caccccggcc gcccccgtgt cgagcgcggc cgccacggac ctgcgcggca
    10921 ccttccggat cacggccggc gcctgcgcag gggcggtcac cggctcgcgg ttccgcatgg
    10981 tgcagccggg cggcacgccg gccagcggcc cgttcgtgga gaacgccgac tcgccctgcg
    11041 cggacaagac ctacaccccc ctgcgcccgg gcacggacgg cggcctggtc agcggcagcc
    11101 accagccgca gccctccgcc ccgttcgacg gcacgggcaa cggcacggcc gcgcgcatcg
    11161 tccggccgca gcgcttcttc ggcgtggact tcgccgtggc caccaacccc acggacccgc
    11221 agagcgggac ggacacgccc gcgccggtga tccgccggga cggctccacg ctgagcggtg
    11281 acctgcgcgc ctacgcggtc gcgtggaacc gccagcactt caaccagggc gcccccaagc
    11341 cggacggctc cacgcccggg cagaccacgc tgcccagcgg caccatcgac gcctccacca
    11401 aggcctaccg gctggagtgg gccagcacca tcgtgggggg gccgttcaac aacttcaccg
    11461 gcctgtggca cctggagggc acgttcgacg gcgcgctggg cgaccaggcc ggaccggtgt
    11521 cccagccgcc ggggtccgtg ccggccccgc ccgccggcgg ca
//
LOCUS       DATMKC010000097         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_85837, whole genome shotgun sequence.
ACCESSION   DATMKC010000097 DATMKC010000000
VERSION     DATMKC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85837"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..274)
                     /locus_tag="VNU01_02930"
     CDS             complement(11..274)
                     /locus_tag="VNU01_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31605.1"
                     /translation="MPVDEKTRNEVFRTLEQKLGQTAAVVIMSVVGAFDPDLLATKAD
                     LVLVKTELTAAEARMSSRLYRALFFQTIAILAANVALIEALAG"
     gene            455..1033
                     /locus_tag="VNU01_02935"
     CDS             455..1033
                     /locus_tag="VNU01_02935"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWH31606.1"
                     /translation="MDETPGLTGLVLAGGRSTRMGRDKALLAWDGTPLVDRVAEVLAE
                     CCDEVLIASGDGTRLGRPHEIADAVPSAGPLGGLLAGMEAAAQPLVAVVAVDMPHASA
                     GVLRALAGALGDAGAAVPLVEGRTHPLHAVYRASCAATVRACLDEGQRSVMGLLARID
                     VREAGPEVWGSADPAGRFATNINRPEEFPRPD"
     gene            complement(1030..1284)
                     /locus_tag="VNU01_02940"
     CDS             complement(1030..1284)
                     /locus_tag="VNU01_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31607.1"
                     /translation="MQLDEQTRHEVFGTLRDKLGETAAVVLMGAIPPFDWSEVATKSD
                     LYAMEERLTDRFTGRMYRAMFFQTLGLFGANVALISAFAR"
     gene            complement(1382..2113)
                     /locus_tag="VNU01_02945"
     CDS             complement(1382..2113)
                     /locus_tag="VNU01_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31608.1"
                     /translation="MLEREWVSITDPTDDHDRYVFDVTFLLSGYRCIYGAGCQGIGPS
                     GVASHGCCSLGAHYADADDRERVEAMAARLGPEFMQFHREAARGGVTARLPGEEFRTR
                     IVQGGCIFLNRQDWPAGAGCALHQYAVARGEHYMTYKPEVCWIVPLRREVVEDVADDG
                     EPLWVTTITSYDRGAWGGGGAAFAWWCIDAPEAYGAPEPVYRSMERELRALSSDAVYE
                     ELAAYLDDRRATARKPLPMLIEQPR"
     gene            2197..>2434
                     /locus_tag="VNU01_02950"
     CDS             2197..>2434
                     /locus_tag="VNU01_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31609.1"
                     /translation="MGYYDDHMDYGGRVLWGRVGVLGGALVLMFLLGRCTAPDGVPPS
                     ELASANAQIQQLESRNAQLQEQIAAMSAGATAEPT"
BASE COUNT          366 a          866 c          858 g          344 t
ORIGIN      
        1 cgcccacccg ctaccccgcg agcgcctcga tcagcgcgac gttggcggca aggatcgcga
       61 tcgtctgaaa aaacagcgca cggtagagac ggctggacat ccgcgcctcg gcggccgtga
      121 gctccgtctt gaccaacacg agatccgcct tggtggccag aaggtcagga tcgaacgcgc
      181 cgacgacact catgatgacg accgccgcag tctgaccaag cttctgctca agggtcctga
      241 acacctcgtt ccgcgtcttc tcatccaccg gcatactggc gctcctcggc ccgcgctgac
      301 aagtgacgcg gcgagtgtcc ccccgttgcc gtgacttcgc tggaaatcca gcgccgcgcc
      361 tgtggacaac gggcgcgtgc cacaggaacg gcgagaaccc acccgcgtca tgcaagaccc
      421 gcggccgtgc cgcccgtggc agtagcgtgc gggcatggac gagacgccgg gcttgacggg
      481 gctggtgctg gcgggcgggc gctccacgcg catgggccgc gacaaggctc tgctggcgtg
      541 ggacgggaca ccgctggtgg accgtgtggc ggaggtcctg gccgagtgct gcgacgaggt
      601 gctgatcgcc agtggcgacg gcacgcgcct gggacgaccc catgagatcg cggacgcggt
      661 gcccagcgcg gggccactcg gcgggctgct cgccgggatg gaagccgccg cacagccgct
      721 ggtggccgtg gtcgccgtgg acatgccgca cgccagtgcc ggggtgctgc gggcgctggc
      781 gggggcgctg ggcgatgcgg gcgcggcggt cccgctggtc gaggggcgga cgcacccgct
      841 gcacgctgtc taccgggcga gctgcgcggc caccgtgcgc gcctgtctgg acgaagggca
      901 gcggtccgtg atggggctgc tggcgcgcat cgacgtgcgc gaggccgggc cggaggtgtg
      961 gggctccgct gatcccgcgg gccgcttcgc cacgaacatc aaccgaccgg aggagttccc
     1021 gcggccggat tagcgcgcga aggccgagat cagcgcgacg ttggccccga acaggcccaa
     1081 ggtctggaag aacatcgcac ggtacatccg gccggtgaac cgatccgtca gccgttcctc
     1141 catggcgtac aggtccgact tggtggcgac ctcgctccag tcgaacggcg ggatggcgcc
     1201 catcagcacc acagccgccg tctcgccgag cttgtcgcgc agcgtgccga acacctcgtg
     1261 acgtgtctgc tcatcgagtt gcatcttcac atccccgggc ccgcgttgac aaggactcga
     1321 acgagtcttg cccaaccgcc gcggctgtgc cggaaatcga gccgcgagac tgtggacaac
     1381 ctcagcgggg ctgctcgatc agcatcggca aaggcttgcg ggcggtcgcc cggcgatcgt
     1441 ccaggtaggc ggccagctcc tcgtacaccg cgtcggagga cagcgcgcgc agctcccgct
     1501 ccatcgagcg gtacaccggc tcgggcgcgc cgtaggcctc cggcgcgtcg atgcaccacc
     1561 acgcgaacgc cgccccgccg ccgccccacg cgccccggtc gtaggacgtt atcgtggtca
     1621 cccacagcgg ctcgccgtcg tcggccacgt cctcgacgac ctcgcgccgc agcggcacga
     1681 tccagcagac ctccggcttg taggtcatgt agtgctcgcc gcgggccacg gcgtactggt
     1741 gcagcgcgca gcccgccccc gccggccagt cctggcggtt caggaagatg cagccgccct
     1801 gcacgatgcg ggtgcggaac tcctcgcccg gcaggcgcgc ggtcacgccg cctcgggccg
     1861 cctcgcggtg gaactgcatg aactccggcc ccagccgcgc cgccatggcc tccacgcgct
     1921 cgcggtcgtc cgcgtcggcg tagtgcgcgc ccagcgagca gcagccgtgc gacgccacgc
     1981 cgctggggcc gatgccctgg cagcccgcgc cgtagatgca gcggtagccg gacagcagaa
     2041 acgtcacgtc gaagacgtag cggtcgtgat catccgtggg gtcggtgatg gacacccact
     2101 cgcgttccag catggtgctc gcctgtcgcg tccgttcggg cctctcgatg gcgagagtat
     2161 gtgcgcgagc gcaaagtcgt ggatacttga ccgccaatgg gctactacga cgatcacatg
     2221 gactacggcg ggcgcgtcct ctggggacgg gtcggcgtcc tgggcggcgc gctcgtgctg
     2281 atgttcctgc tgggccgctg caccgccccc gacggggtgc ccccgagcga gctcgcgtcg
     2341 gccaacgccc agatccagca gctggagtcg cgcaacgccc agctgcagga gcagatcgcg
     2401 gccatgtccg ccggtgcgac ggccgagccc accg
//
LOCUS       DATMKC010000098         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_279599, whole genome shotgun sequence.
ACCESSION   DATMKC010000098 DATMKC010000000
VERSION     DATMKC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279599"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1010
                     /locus_tag="VNU01_02955"
     CDS             324..1010
                     /locus_tag="VNU01_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH31610.1"
                     /translation="MSRQDGDIGALAGGTVRQELGDAERQELFSREALPYVDQLYSAA
                     LRYTRNPADAEDLVQEAFLKAYAAFHQFEPGTNLRAWLYRILTNAYINRYRKEQRRPS
                     EVSADAWAARVTEDGGGDFSLYDRIAETTERSAEVEALSHLTDEEVKRALADLPEQFR
                     MAVYLADVEGFSYAEIADIMGTPIGTVMSRLHRGRAALQRALYSYAVERGLVAPTESV
                     TEAATGSQTR"
     gene            1007..1312
                     /gene="rsrA"
                     /locus_tag="VNU01_02960"
     CDS             1007..1312
                     /gene="rsrA"
                     /locus_tag="VNU01_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HWH31611.1"
                     /translation="MTDQCGTPRPPEEFDPSAVARPMLDSCREVLEELDLFLDNECGD
                     DLAASVRRHLGHCPPCMDRADFERALRELIASKCRDAAPSGLVDRIIDSLKPASPPA"
     gene            1309..2199
                     /locus_tag="VNU01_02965"
     CDS             1309..2199
                     /locus_tag="VNU01_02965"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWH31612.1"
                     /translation="MTRILVTGGAGVIGSHLVDRLIAEDHYVTVADDLSSGRLHYLQD
                     ARRHPDKRLTFQRVDVTSTALERTVAKARPEVVVHLASRADPQASDDPTRDALTDVVG
                     TVNLIEACRAHDVGKIVFAGHLDDRRAAPPTAFLAGKRAAEEYLRALGRSGPRWTVLS
                     IAEVYGPRQDPAVPRHLVARLADWMRERAPVTLAGGPERAWDLVAVDDVVEALVLAMS
                     AGDGLRIPVGSGRPTTQGEVVATLAALTAWTGEPHWLPGDGTEPPLLADVERAGEALG
                     WSPRVGLEDGLRALLEWLRG"
     gene            2214..>2827
                     /locus_tag="VNU01_02970"
     CDS             2214..>2827
                     /locus_tag="VNU01_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWH31613.1"
                     /translation="MAEAAVRTELGGGVLRVTIDRPDQRNALNADVNRGLLEALARLR
                     ADGDAKVMVLTGAGDKAFCAGADLGGLNPDDGAVALHRGRSLFADVLRELRSVPKPVV
                     GRVNGHALAGGFGLALGCDLLVAADHATFGTTEVKVGMWPYMITAVIVEHLGPKRTME
                     LMMTGERIDAARAREWGLVNRVAAAADLDAATDELVESLLALSPA"
BASE COUNT          405 a         1049 c         1030 g          343 t
ORIGIN      
        1 ccgtcgcgca gccagtcgcc catcggcccc gcggcgccct gcggcccgcc gccaccgggc
       61 gcgccgccac cgggtgcccc ggcgccgtgc gccccggcgc tcggggggcc ggcccagccg
      121 aacgcgccgc ccgcggagcc ctgcgcgtcg tggtagcccg catccgtcat cgcccacccc
      181 cgttgccgtg ccttgccgtc agtgcagcta acaacaagga ttaagaagtg acaagacgca
      241 acaacatcaa gctgtggata ttcgcgcggc agggagccac tgcctagact cagcgcacgt
      301 cggcacagtg gcaggagccc ggaatgagca ggcaggacgg cgacatcggc gccctcgcgg
      361 ggggcacggt gcgccaggag ctcggcgacg cggaacggca ggagctgttc tcgcgcgagg
      421 cgctcccgta cgtcgaccag ctgtactccg cggcgctgcg ctacacccgc aacccggccg
      481 acgccgagga cctggtgcag gaggcgttcc tgaaggccta cgccgccttc caccagttcg
      541 agcccggcac gaacctgcgc gcgtggctgt accggatcct caccaacgcc tacatcaacc
      601 ggtaccgcaa ggagcagcgg cgcccctccg aggtgtccgc cgacgcgtgg gccgcccggg
      661 tcaccgagga cggcggcggc gacttttccc tgtacgaccg catcgcggag accaccgagc
      721 gctccgcgga ggtcgaggcg ctgagccatc tcaccgacga ggaggtcaag cgggcgctgg
      781 cagacctgcc ggagcagttc cgcatggccg tgtacctggc cgacgtggag ggcttctcct
      841 acgccgagat cgcggacatc atggggacgc ccatcggcac ggtcatgagc cggctgcacc
      901 ggggaagagc agcgctgcag cgcgcgttgt actcctacgc cgtggagcgc ggactggtcg
      961 ccccgacgga gagcgtgacg gaagccgcca caggaagcca gacgcgatga ctgaccaatg
     1021 cgggacccca cgcccgccgg aggagttcga tccctcggcg gtcgcgcggc cgatgctgga
     1081 cagctgccgc gaggtgctgg aggagctcga cctgttcctg gacaacgagt gcggcgacga
     1141 cctcgccgcc agcgtccgcc gccacctggg ccactgcccg ccgtgcatgg accgcgccga
     1201 cttcgaacgc gcgctgcgcg agctgatcgc ctccaagtgc cgtgacgccg ccccgtcggg
     1261 cctggtggac cgcatcatcg acagtctgaa gccggccagc ccgcccgcat gacgcggatc
     1321 ctggtcaccg gcggcgccgg cgtcatcggc tcgcatctcg tggaccggct gatcgccgag
     1381 gaccactacg tcaccgtcgc cgacgacctg tccagcggca ggctgcacta cctccaggac
     1441 gcccgccgcc acccggacaa gcgcctgacg ttccagcgcg tcgacgtcac gtccacggcg
     1501 ctggagcgca cggtcgccaa ggcccgtccc gaggtggtgg tgcacctggc ctcgcgcgcc
     1561 gacccgcagg cctccgacga ccccacgcgc gacgcgctga ccgacgtggt gggcaccgtg
     1621 aacctgatcg aggcgtgccg cgcgcacgac gtcggcaaga tcgtcttcgc cggccacctc
     1681 gacgaccgcc gcgccgcgcc gcccacggcg ttcctggccg gcaagcgcgc cgccgaggag
     1741 tacctgcgcg cgctcgggcg gtccggtccc cgctggacgg tgctgtccat cgccgaggtg
     1801 tacggcccgc gtcaggaccc ggcggtgccc cgccacctcg tcgcccggct ggccgactgg
     1861 atgcgcgagc gggcccccgt gacgctggcc ggcggacccg agcgagcctg ggacctggtc
     1921 gccgtcgacg acgtcgtgga ggcgctggtc ctggcgatgt ccgcgggcga cggcctgcgc
     1981 atccccgtgg gcagcggccg gccgacgacg cagggcgagg tggtggccac gctggccgcg
     2041 ctcaccgcct ggaccggtga gccgcactgg ctgcccggtg acggcacgga gccgccgctg
     2101 ctggccgacg tcgagcgcgc cggcgaggcg ctgggctggt cgccgcgcgt gggcctggag
     2161 gacggactgc gggcgctgct ggagtggctg cgcggctaga caaggagagc gacgtggcgg
     2221 aagcggcagt gcggaccgag ctcggcggcg gggtcctgcg ggtgaccatc gaccgccccg
     2281 accagcgcaa cgccctcaac gccgacgtga accgcggcct gctcgaggcg ctggcgcggc
     2341 tgcgcgccga cggcgacgcc aaggtcatgg tgctcaccgg cgcgggcgac aaggcgttct
     2401 gcgcgggcgc ggacctgggc gggctgaacc ccgacgacgg ggcggtcgcg ctgcaccgcg
     2461 gacggtcgct gttcgccgac gtgctgcgcg aactgcgcag cgtgcccaag ccggtcgtcg
     2521 gacgggtgaa cggccacgcg ctggcgggag gcttcggcct ggcgctgggc tgcgacctgc
     2581 tcgtcgccgc cgaccacgcc accttcggca ccaccgaggt gaaggtcggc atgtggccgt
     2641 acatgatcac ggcggtcatc gtcgagcacc tgggccccaa gcggacgatg gagctcatga
     2701 tgaccggtga gcgcatcgac gcggcgcgcg cgcgcgagtg ggggctggtc aaccgcgtcg
     2761 ccgcggccgc cgacctcgac gcggcgaccg acgagctcgt cgagagcctg ctggcgctgt
     2821 ccccggc
//
LOCUS       DATMKC010000099         4401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_307352, whole genome shotgun sequence.
ACCESSION   DATMKC010000099 DATMKC010000000
VERSION     DATMKC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307352"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VNU01_02975"
     CDS             <1..304
                     /locus_tag="VNU01_02975"
                     /inference="COORDINATES: protein motif:HMM:NF026044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31614.1"
                     /translation="LRESVQVRALPDNLPSSLELDITSLESFDDVLHVSDLSVPEGVT
                     VLTDPGESLARVQAPRVEEAAVPGVEEGAPEEGAEGEAAEGGAEAGSAEAADTDAS"
     gene            complement(513..1169)
                     /locus_tag="VNU01_02980"
     CDS             complement(513..1169)
                     /locus_tag="VNU01_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH31615.1"
                     /translation="MAGRERRIERSAWLMRRELADAGAELRNARLAAGLTLRDVADAI
                     GVSASFVLKCERGLPPGARPEVLATHAAAVGMRARIRVYPDGEPLRDAAQLRLLARLR
                     ERIGGRAVLRAEQPVTTDPADRRAFDAVLELPGCRCAIECISRLHDCQAQLRQLHLKQ
                     RDGGVDRLIVVVGATRHNRRALREIEAAVAPTFPLDTRQVLRALRDGRDPGANGIILL
                     "
     gene            1243..2391
                     /locus_tag="VNU01_02985"
     CDS             1243..2391
                     /locus_tag="VNU01_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWH31616.1"
                     /translation="MRIVGLVCALLIAACSGGTTSPPPIETATPMPSLTVLPPGPTAT
                     PTIQPATPTIDRPTASPVSTPGLGEGSVAWSRLELDGGPSAREDHTFTADADGRFAYL
                     FGGRAGNRAFNDLWRYDLATDAWQLIEPRGPAPEPRFGHVAVWQPELGLVVWSGQQSA
                     RVFFGDIWSFDPGTEQWQELPDAGDVPEARYGSCGSIGPDGRLWISHGFTEDTGRFFD
                     TRAYDFGRLAWTDETPADGDVPVLRCLHDCLWTPDGELVLYGGQTTGAPAIGDLWAYG
                     FVSGAWAEAERPTAPPRQLYALASIGSAAFVFGGAGADGALLDDLWRLDLTGLTMSEA
                     DVSGAEGPGARLGATLLADEARGRMILFGGKGEAGELADLWILSPAGS"
     gene            complement(2399..3880)
                     /locus_tag="VNU01_02990"
     CDS             complement(2399..3880)
                     /locus_tag="VNU01_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31617.1"
                     /translation="MDSRTTQRMAALALLTFIFAAAAFMPAADAAHAGPDHACPFCAT
                     RPQPIAAMPQPEAYPTPAATQAATAVTLGATTVRLRAAVYQVYRFGARGKLLEQRTVR
                     FASPRVWRAPAEEVTLGTGRYVKLASGPYSGWYVPAASTVSAATTHFTPHLNVTVRSG
                     RLVARRFFGPVVTRRMATLATTATFKSSKRALFNGATHYLIETGPLAGRWVAASRIIV
                     PSATDSQKATPTPAASPTASAQPTASAQPSQTAQPTVAPAAGPLSTWKTVVLLYRETD
                     LTFKRADGSDYQLKARMNDTMYNLVRKTLGQFEKSAGTWSGELAAMDMTILDMPNPLT
                     RVDALGSSYWVGPNTIQADLDKYAPKGKWDSVFVIYQPKDDKGVSIPIGGWGLTIPDG
                     SWSNNTGFSSVVTPHELWWWTGSSAPEEVFVHEWMHQVLFFHERAGRTNLDLHAGDKF
                     GYKDKDGSWKTWLMDVMQGKVKDGSKTIGVSADVWASGKPSAP"
BASE COUNT          605 a         1504 c         1592 g          700 t
ORIGIN      
        1 cctgcgcgag tcggtccagg tgcgcgccct gccggacaac ctgccttcat cgctcgagct
       61 ggacatcacg agcctcgagt cctttgacga cgtgctgcac gtctctgatc tctccgtgcc
      121 ggagggcgtg accgtcctca ctgaccccgg cgagtcgctg gcccgcgtgc aggcgccgcg
      181 cgtggaggag gccgccgtgc ccggcgtcga ggagggagcg cctgaggagg gcgctgaggg
      241 cgaggccgcc gagggcggcg ccgaggctgg cagcgccgag gccgcggaca cagacgcgag
      301 ctagggctcc cagggccgat tctccgcgcg cgcgccggct gttgttctcc cggcgtgaag
      361 taacgacaag aagcgcagcg actaccgcgc gtgaaggctc ttggcggttc gcctgtgcac
      421 gccgctcgaa caaacggccc acggaccggg gaaagaacgg gtgcgcggcc cagatcttcg
      481 ccgttagctc acccgcggtg aacgggcggc tgctacagca ggatgatgcc gttggcgcct
      541 gggtcgcgac cgtcgcgaag tgcgcgcagg acctggcgag tgtccagcgg gaaggtgggc
      601 gccaccgcag cctctatctc gcgcagcgca cgccggttgt ggcgcgtggc gcccaccacc
      661 acgatcagcc gatccactcc tccgtcacgc tgcttgaggt gcagctgccg cagctgtgcc
      721 tggcagtcgt gcaggcggct gatgcactcg atcgcgcagc ggcagccggg caactccagg
      781 accgcgtcga acgcgcgccg gtcagccgga tctgtggtga ccggctgctc cgcgcgtaga
      841 accgcccgcc cgccgatgcg ctcgcggagc ctggccagca gccgcagctg ggcggcgtcg
      901 cgcagcggct cgccatctgg gtagacccgg atccgggcac gcatcccgac tgccgcggca
      961 tgtgtggcca acacctccgg cctggctccg ggtggcaggc cgcgctcgca tttgagcacg
     1021 aacgaggccg agacaccgat tgcgtccgcg acgtcgcgca gcgtgagccc agctgccagc
     1081 ctggcgttgc gcagctccgc gccggcgtca gccagctcgc gccgcatcag ccacgccgag
     1141 cgttcgatgc gtcgctccct gcccgccatg cctcgcagcc tgacgatccg tgcttatcgg
     1201 ccgctttgcc gccgattgat tacgctgtcg gcggtgccgt gcatgaggat cgtcggcctg
     1261 gtctgcgcgc tgctgattgc cgcgtgcagc ggcggcacga cctcgccgcc cccgatcgag
     1321 accgccaccc caatgccgag cctcaccgtc ctgcctcccg gcccgactgc gaccccgaca
     1381 atccagccgg cgacgccgac catcgaccgg cccacggcca gccccgtttc aacacccggg
     1441 ctcggcgagg gcagcgtcgc ctggtcgagg ctggagctcg atggcggccc gtcggcgcgc
     1501 gaggaccaca ccttcacggc tgacgcagac ggccgcttcg cgtacctgtt cggcggccgt
     1561 gccggcaacc gggcattcaa cgacctgtgg cgctacgacc tggcgaccga tgcctggcag
     1621 ctgatcgagc cgcgcggccc agcaccggag ccgcgcttcg gccacgtcgc cgtctggcag
     1681 ccagagcttg gcctcgtggt gtggagtggc cagcaatcgg cgcgcgtctt cttcggcgac
     1741 atctggtcgt tcgatccggg cacggagcag tggcaggagc tgcccgatgc gggcgacgtt
     1801 cccgaggcgc gctacggctc gtgcggctcg atcgggccgg atggccggct gtggatcagc
     1861 cacggcttca ccgaggacac gggccgcttc ttcgacaccc gcgcgtatga cttcgggcgc
     1921 ctcgcctgga ccgacgagac accggctgac ggagacgtgc cggtgctgcg ctgcctgcac
     1981 gactgcctgt ggacgcctga tggcgagctg gtgctgtatg gcgggcagac gaccggcgca
     2041 ccggcgatcg gcgacctgtg ggcctacggg ttcgtgtccg gtgcctgggc cgaggctgag
     2101 cgcccgactg ctcctcctcg ccagctctac gcgctggcat ccatcgggtc ggcggccttc
     2161 gtctttggtg gcgcgggcgc ggacggcgcg ctgctcgacg acctgtggcg gctcgacctg
     2221 accggcctga cgatgagtga ggcagatgtg agcggcgctg aaggcccggg tgcgcgcctt
     2281 ggcgcgacgc tgctggcgga cgaggcgcgc ggccggatga tcctcttcgg cgggaagggt
     2341 gaggccggcg agctggccga cctgtggatc ctgtccccgg caggctccta ggcttggctc
     2401 agggcgcgct cggcttgccg gaggcccaga cgtcggcaga gacgccaatc gtcttgctgc
     2461 cgtctttgac cttgccctgc atgacgtcca tcagccaggt cttccagctg ccgtccttgt
     2521 ccttgtagcc gaacttgtca ccggcgtgaa ggtcgaggtt cgtgcggccc gcgcgctcgt
     2581 ggaagaagag cacctggtgc atccactcgt ggacgaagac ctcctccggt gctgacgagc
     2641 cggtccacca ccagagctcg tgcggcgtga cgaccgagct gaaaccggtg ttgttcgacc
     2701 acgagccgtc cgggatggtc agcccccagc cgccgatcgg gatcgacacg cccttgtcgt
     2761 ccttgggctg gtagatcacg aagacgctgt cccacttgcc cttgggcgcg tacttgtcga
     2821 gatcggcctg gatggtgttc gggccgaccc agtagctgct gcccagggca tcgacgcggg
     2881 tcagcggatt gggcatgtcg aggatggtca tgtccatcgc cgccagctcg cccgaccagg
     2941 tcccggccga cttctcgaac tggccgagcg tcttgcgcac gaggttgtac atggtgtcgt
     3001 tcatccgcgc cttgagctgg tagtcggagc cgtcagcgcg cttgaacgtg aggtccgtct
     3061 cgcggtacag cagcaccacc gtcttccagg tgctgagcgg gccggccgcc ggggcgaccg
     3121 tcggctgagc ggtctgggag ggctgggctg aggccgtcgg ctgagcggag gcggttggtg
     3181 atgcggcggg agtgggcgtg gctttctgcg aatcggtcgc gctgggcacg atgatccggc
     3241 tggcggccac ccagcgcccg gcgagcgggc cggtctcgat gaggtaatgg gtggcgccgt
     3301 tgaacagcgc acgcttggac gacttgaagg ttgccgtcgt tgccagcgtg gccattcgcc
     3361 gggtcacgac cgggccgaag aatcgccgcg cgacgagccg ccctgaccgc accgtgacat
     3421 tcaggtgcgg agtgaaatgg gtcgtcgctg cggacactgt gctggcggct ggcacgtacc
     3481 agccgctgta cgggccgctg gccagcttga cgtagcggcc ggtgccgagc gtgacctcct
     3541 cggccggcgc gcgccagaca cgcgggctgg cgaagcggac cgtgcgctgc tcgagcagct
     3601 tgccgcgcgc gccaaagcga tagacctggt agacagccgc gcgcagccgg accgtcgtcg
     3661 cgccgagcgt caccgccgtg gcggcctggg tcgcggcggg agtgggatac gcctcgggct
     3721 ggggcatcgc ggcgattggc tggggccggg tggcgcagaa cggacatgcg tggtccggcc
     3781 ctgcgtgcgc ggcgtcggcg gccggcatga aggccgcggc cgcgaagatg aaggtcagga
     3841 gcgcgagcgc tgccatgcgc tgcgtggtcc ggctgtccac gacctatgaa tccctccgct
     3901 ccagcccggt gcggggctgg cagtgcgcgc gacgtgtcga gttcgcactt cggagagtcg
     3961 gagcggaggt ggcgcggcag tatcacccct aaggactggc ctccggggag aagggtccta
     4021 gcgctggggc gtcgggtggg gtcttgggcg gtgtcagctg cggcccgccg gtccctacat
     4081 gccaccacaa ccgccagcag cgttgctcac cgcccctgtc gtgcgtgtcg cttccgggta
     4141 cggaacgcgc gacccgtggc cggatcagtg gcttccggat tgatccatgg aaccggagca
     4201 gccgacactg cgtccaagcg ccagacgagg agattccctc cctccccgtc gacgccacag
     4261 gagaaaccct ctctctcctg ctgaaggggc cgggcctgct gcgctggggc gcgcgcccgg
     4321 ccccttcgtt tctcccgcgc ggtcaaagtg aaagccgggc cgactttcgg cggcccggct
     4381 tcagacgggc tgggtgtgat c
//
LOCUS       DATMKC010000100         3107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_156669, whole genome shotgun sequence.
ACCESSION   DATMKC010000100 DATMKC010000000
VERSION     DATMKC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156669"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="VNU01_02995"
     CDS             complement(<1..1782)
                     /locus_tag="VNU01_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH31618.1"
                     /translation="MAESGSQGPWDGMRGRARVATLRTLADVWVPGLRPPPGADEPLA
                     AFWRRSASELGVEHAVARYIQERLGVQDRDGLVRLLDLLTVLAPRVGPPAVRERVLAG
                     IRRLHPDVAAGLDALRALTLLHFYAQPGPDGRNPNWPALGYAGPPQATPPAGDRLPTV
                     TPAAGAERLELEADACVIGSGAGGGVIAATLAQAGLAVVVIEAGAHREEPDFRPYELE
                     AYRDLYWRGGYQATEDRTVSLIAGATLGGGTVVNWMNCVAPPDFVREQWAGLGLAGLD
                     GADFDRHLDAVRARISANTDCSDFNGPNARLAEGAKALGWSWTVAARNADPATYDPET
                     AGHMGFGDRSGSKQSTLRTYLRDAVAAGARVVPSARAARVLVRDGRAAGVEGSVARPD
                     GGTAELRVRASLVVAACGALETPALLLRSGVGGPAVGRHLRLHPVDALAGLYGEEQRA
                     WWGAPQTVIVDEHRDLADGHGFLLEAPHFGTGLSAASVPWTGGRAHKELVARSARMAT
                     FIGVVRDRGEGRVTVDDGGEAVVAYPLDDALDRAHLRRALEEMARLHEAAGAAEILDL
                     SPGLPRWRRGDGDLEVWLSRVGDLPMGA"
     gene            1967..2146
                     /locus_tag="VNU01_03000"
     CDS             1967..2146
                     /locus_tag="VNU01_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31619.1"
                     /translation="MPDLWAYCSPCARWFYCARSGRDPVDECPACGVPSRTRQDRHPA
                     RATADAPDRDATQES"
     gene            2197..2274
                     /locus_tag="VNU01_03005"
     tRNA            2197..2274
                     /locus_tag="VNU01_03005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2232..2234,aa:Pro,seq:tgg)
     gene            2307..>3107
                     /locus_tag="VNU01_03010"
     CDS             2307..>3107
                     /locus_tag="VNU01_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31620.1"
                     /translation="MTGSLDKSRAGRRPLRNAVLAAVMLLALACAGTPALAQEACEDF
                     DDVAGANPAAAAVWAEIDAAARAARVPPALLAAVAFAEGYDEATARNWIQFRADGRPI
                     VSFDCGIGMLQLTAADGYDARRLAADWRYNVRAGAAVLRAKWDASQANAPDGLERDER
                     FAENWWAALCRYNGQGAAATAYADRAMAFATDPLGEAAVFTPPLRTLRTPSDVFPAFT
                     PSHWYVVRDGEAILATAEGDVVERRAGFVPTALDASLAGPARPPAPRVG"
BASE COUNT          363 a         1230 c         1144 g          370 t
ORIGIN      
        1 cgcgcccatc ggcaggtcgc ccacgcgcga cagccagacc tccaggtcgc cgtcgccgcg
       61 ccgccagcgg ggcagtccgg gtgacaggtc caggatctcg gcggcgcccg ccgcctcgtg
      121 cagccgggcc atctcctcca gcgcccggcg caggtgggcg cggtccagcg cgtcgtccag
      181 cgggtaggcc acgaccgcct cgccaccgtc gtcgacggtg acgcgcccct cgccgcggtc
      241 gcgcaccacg ccgatgaacg tggccatgcg ggcgctgcgc gccaccagct ccttgtgcgc
      301 gcgcccgccg gtccagggca cgctggcggc cgacaggccg gtgccgaagt gcggcgcctc
      361 cagcaggaag ccgtggccgt cggccaggtc gcggtgctcg tccacgatca cggtctgggg
      421 cgcgccccac caggcgcgct gctcctcgcc gtacaacccg gccagcgcgt ccaccgggtg
      481 caggcgcagg tgccggccca ccgcgggacc gccgacgccg gagcgcagca gcagcgccgg
      541 tgtctccagc gcgccgcagg ccgccacgac cagggacgcg cggacgcgca gctccgccgt
      601 gccgccatcg ggacgggcga cgctgccctc caccccggcc gcccggccgt cgcgcaccag
      661 cacgcgcgcc gcgcgcgcgc tcggcaccac ccgggcaccg gcggccaccg cgtcccggag
      721 gtaggtccgc agcgtgctct gcttgctgcc ggagcggtcg ccgaagccca tgtggccggc
      781 ggtctcgggg tcgtaggtgg ccgggtcggc gttgcgcgcc gcgacggtcc atgaccagcc
      841 gagcgccttg gcgccctccg ccagccgggc gttgggcccg ttgaagtcgc tgcagtcggt
      901 gttggcggag atgcgcgccc gcaccgcgtc caggtggcgg tcgaagtcgg cgccgtcgag
      961 gccggccagc cccaggccgg cccactgctc gcgcacgaag tccggcggcg ccacacagtt
     1021 catccagttg accaccgtgc cgccgcccag cgtcgcgccg gcgatcaggc tgaccgtgcg
     1081 gtcctcggtg gcctggtagc cgccgcgcca gtacaggtcg cggtaggcct ccaattcgta
     1141 cgggcggaag tccggctcct cgcgatgggc gcccgcctcg atcacgacca ccgcgagccc
     1201 ggcttgcgcc agggtggcgg cgatgacgcc gccacccgcg ccggacccga tgacgcaggc
     1261 gtcggcctcc agctccaggc gctcggcgcc ggccgcaggc gtcaccgtgg gcagccggtc
     1321 cccggccggc ggtgtggcct gaggcgggcc cgcgtagccc agcgccggcc agttcgggtt
     1381 tcgcccgtcc ggccccggct gggcgtagaa gtgcagcagc gtcagcgcgc gcagcgcgtc
     1441 caggccggcc gccacgtcgg ggtgcaggcg gcggatgccc gcgagcaccc gttcgcgcac
     1501 ggccggcggt cccacgcgcg gcgcgagcac ggtgagcagg tccagcaggc gcaccagccc
     1561 gtcacggtcc tgcacgccga ggcgctcctg gatgtagcgc gcgacggcgt gctccacgcc
     1621 cagctcggaa gccgagcgcc gccagaatgc ggccagtggc tcgtccgcgc ccggcggcgg
     1681 ccgcagcccc gggacccaca cgtcggccag cgtgcgcagc gtcgccacgc gggcgcgccc
     1741 gcgcatgccg tcccaggggc cttgcgagcc ggactccgcc atgtcgtgac ctcctgaggt
     1801 ggcgccgcgg gggcaggagt ctagagcgcg agcgaatcgg ccggaggcgt caaaaccatc
     1861 cctgagctct gcaatatctg ttaccggcgg tatgttcggc tctcgctcgt gaccccggcg
     1921 gccccatttc cctcgtcgtg gccccttggg ggcaggagtg tttggcatgc cggatctctg
     1981 ggcctactgc tcgccgtgcg cgcgctggtt ctactgcgcg cggtccgggc gtgaccccgt
     2041 ggacgagtgc cccgcctgcg gcgtgccctc ccgcacgcgc caggacaggc accccgcccg
     2101 cgccaccgcg gacgcgcctg accgggacgc cacccaggag tcgtgagccg cggccgccgc
     2161 ctacactggt cgtcggcgcc cgcacgcggg caccgacggg atgtggcgca gcttggttag
     2221 cgcgcgtgct ttgggagcac gaggccccgg gttcgaatcc cggcatcccg accacgctcg
     2281 cgtcagaccg gggctacgct ccgcccatga ccgggtccct cgacaaaagc cgtgccggca
     2341 ggcgcccctt gcgcaacgcc gtgctcgccg ccgtgatgct gctcgcgctg gcgtgcgcgg
     2401 gcaccccggc gctggcccag gaggcgtgcg aggacttcga cgacgtcgcc ggcgcgaacc
     2461 cggccgccgc cgcggtgtgg gccgagatcg acgcggccgc ccgcgcggcc cgtgtccccc
     2521 cggcgctgct ggcggcggtc gccttcgcgg agggctacga cgaggccacc gcgcgcaact
     2581 ggatccagtt ccgcgccgac ggcaggccga tcgtgtcgtt cgactgcggc atcggcatgc
     2641 tccagctcac cgccgcggac ggctacgacg cgcggcggct ggcggcggac tggcgctaca
     2701 acgtgcgggc cggcgccgcc gtgctgcgcg ccaagtggga cgcctcccag gccaacgccc
     2761 ccgacggcct ggagcgcgac gagcgcttcg cggagaactg gtgggcggcg ctgtgccgct
     2821 acaacggtca gggcgccgcc gccacggcct acgccgatcg cgccatggcg ttcgccaccg
     2881 accccctcgg cgaggcggcc gtgttcacgc cgccgctgcg cacgctgcgc acgccgagcg
     2941 acgtcttccc ggccttcacc cccagccact ggtacgtggt gcgcgacggg gaggcgatcc
     3001 tggccaccgc cgagggcgac gtggtggagc gacgcgccgg cttcgtgccg accgcgctgg
     3061 acgcgagcct ggccggcccg gcacggccgc ccgcgccgcg cgtgggc
//
LOCUS       DATMKC010000101         3833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_213485, whole genome shotgun sequence.
ACCESSION   DATMKC010000101 DATMKC010000000
VERSION     DATMKC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213485"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /gene="cimA"
                     /locus_tag="VNU01_03015"
     CDS             <1..1582
                     /gene="cimA"
                     /locus_tag="VNU01_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HWH31621.1"
                     /translation="YDTTLRDGTQQEGISLSVADKLRVASVLDTLGVNFIEGGWPGAN
                     PKDTEFFKRAASGELALRRATLTAFGMTRRAHVDASGDPGLLTLLEAQTPVVCLVGKA
                     DLLHVTEALGTDRDEAVRMVADSVAFLRGEGRRVFFDAEHFFDGHRRDASFALDVVTA
                     AAEAGAECVVLCDTNGGALPEDVDRVVREVAGRVGGASQVGIHVHNDADCAVANSMVA
                     VRAGATHVQGTANGIGERCGNANLMSLIPNLKLKLGLDVIGDDDLRRLTAVAHEVAEV
                     MNLTLDPHAPYVGSAAFAHKAGLHVSALAKNTDLYQHVPPESVGNTLRLLVSELAGRS
                     TVVLKGKDFGVDLSTDGGAVTRILDRVKDLEHIGYSFEAADASFELLVRQETGTFREF
                     FRLESFRTIVTRDETGQVKAEAIVKLWVDDERLMAVGEGNGPVSALDAAFRTAVNGRY
                     PALASVHLADYKVRIIDPGSGTGATTRVLVTSTDGEREWETVGVSGNIIDASWKAMQD
                     AYTYALLHAESGGGPEGS"
     gene            1582..2355
                     /locus_tag="VNU01_03020"
     CDS             1582..2355
                     /locus_tag="VNU01_03020"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWH31622.1"
                     /translation="MRIVRVARPDGPAFGVVRDTEQGEVVELIAPHPFAEFAGTGQVA
                     PLERVKLLAPVIPTKILAIGRNYADHAAEMGGEVPPEPLVFAKLPTTVIGPDDAIVLP
                     ELSGEVHHEAELAVVVKRIARQVPVERAHEAILGYTCANDVTARDLQRTDGQWTRAKG
                     FDTFCPLGPWISTDLDLADAAVRCRVNGQTRQDGSTRDLVFSIPQLVSHLSAFTTLLP
                     GDVILTGTPAGVGELRDGDTVEVEVGGIGVLRNPVRAEA"
     gene            complement(2407..2799)
                     /locus_tag="VNU01_03025"
     CDS             complement(2407..2799)
                     /locus_tag="VNU01_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31623.1"
                     /translation="MNALLRATERVPNWFRAAAMVVLPFLAYWAATRKAPLPSAVFVG
                     WSLVTLLVHLPVMFFDGPLPVSELGVHHSGEIHPWEDVVAVEPLGEKDLRAVLADGRT
                     VKLRVRGGRTRADMLAAIAAYRPELAKG"
     gene            2867..>3833
                     /locus_tag="VNU01_03030"
     CDS             2867..>3833
                     /locus_tag="VNU01_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWH31624.1"
                     /translation="MTAPRVRFCPAPSGWLHVGGARTALYNWLYARQAGGAFILRIED
                     TDADRATRESAEAMMEGLRFLGLDWDEGPGHTGAHGPYFQSERTDLYQAVAHALLQAG
                     HVYPAFETEEELKAQREQAMKEGRPPGYSGAHRDLTQEQIDAFVAEGRTPVLRVRTPD
                     AGVIGFDDAVRGQVEFDWKDIPDFVVQRADGSTVYFLANAVDDLAMGVNFVARGEDLL
                     SATPRQLLLYELLTHDGLIDRVLADLGYPARPDGAMVPPRFAHLPLLVGEDRKPLSKR
                     HGSVAIEEFRRQGFLPEVLLNFLALCGWSYEGVRERFTVDELVEKF"
BASE COUNT          546 a         1376 c         1396 g          515 t
ORIGIN      
        1 gtacgacacc acgctgcgcg acggcaccca gcaggagggc atctccctgt cggtggccga
       61 caagctgcgc gtggcgtcgg tcctggacac cctgggtgtg aacttcatcg agggcggctg
      121 gcccggcgcg aacccgaagg acaccgagtt cttcaagcgg gccgcgtccg gcgagctggc
      181 gctgcggcgc gccacgctga cggcgttcgg catgacccgg cgggcccacg tcgacgcctc
      241 cggcgacccg ggcctgctga cgctgctgga ggcgcagacg cccgtggtgt gcctggtcgg
      301 caaggccgac ctgctgcacg tcacggaggc gctgggcacc gaccgggacg aggcggtgcg
      361 catggtggcc gactccgtgg cgttcctgcg cggcgagggc cggcgcgtgt tcttcgacgc
      421 cgagcacttc ttcgacgggc accgccgcga cgcgtccttc gccctggacg tggtgacggc
      481 cgccgcggag gccggcgcgg agtgcgtggt gctgtgcgac acgaacggcg gcgcgctgcc
      541 ggaggacgtc gaccgcgtgg tgcgcgaggt ggccggccgc gtcggcggcg ccagccaggt
      601 cggcatccac gtgcacaacg acgccgactg cgccgtggcg aactccatgg tcgccgtgcg
      661 cgcgggggcg acgcacgtgc agggcacggc caacggcatc ggcgagcgct gcggcaacgc
      721 caacctcatg tcgctgatcc cgaacctcaa gctgaagctg ggcctggacg tcatcggcga
      781 cgacgacctg cggcgcctca ccgccgtggc gcacgaggtc gcggaggtca tgaacctgac
      841 gctggacccg cacgcgccgt acgtgggcag cgccgcgttc gcgcacaagg ccgggctgca
      901 cgtctcggcc ctggccaaga acaccgacct gtaccagcac gtgccgccgg agtcggtggg
      961 caacacgctg cgcctgctgg tgtcggagct ggcggggcgg tccaccgtgg tgttgaaggg
     1021 caaggacttc ggcgtggacc tgtccaccga cggcggcgcc gtcacgcgca tcctcgaccg
     1081 ggtcaaggac ctggagcaca tcggctacag cttcgaggcg gccgacgcgt ccttcgagct
     1141 gctggtgcgc caggagaccg gcacgttccg cgagttcttc cggctggaga gcttccgcac
     1201 catcgtcacg cgcgacgaga cggggcaggt caaggccgag gcgatcgtca agctgtgggt
     1261 ggacgacgag cggctcatgg cggtggggga gggcaacggc ccggtgtccg ccctggacgc
     1321 cgcgttccgc acggcggtca acgggcgcta ccccgcgctc gcgtccgtgc acctggccga
     1381 ctacaaggtg cgcatcatcg acccgggctc cggcacgggc gcgaccacgc gcgtgctggt
     1441 gacgtccacg gacggggagc gcgagtggga gaccgtcggg gtctccggca acatcatcga
     1501 cgcctcctgg aaggcgatgc aggacgccta cacctacgcg ctgctgcacg ccgagtcggg
     1561 cggcgggccg gagggctcct gatgcgcatc gtgcgggtgg cccggccgga cggcccggcg
     1621 ttcggcgtgg tgcgcgacac cgagcagggc gaggtggtgg agctgatcgc gccgcacccg
     1681 ttcgccgagt tcgccggcac cgggcaggtg gcgccgctgg agcgcgtcaa gctgctcgcg
     1741 ccggtgatcc ccaccaagat cctggccatc ggccgcaact acgccgacca cgcagccgag
     1801 atgggcggtg aggtgccgcc cgagccgctg gtgttcgcca agctgccgac cacggtgatc
     1861 ggcccggacg acgccatcgt gctgcccgag ctgtccggcg aggtgcacca cgaggcggag
     1921 ctggccgtgg tggtcaagcg catcgcccgc caggtgccgg tggagcgcgc ccacgaggcg
     1981 atcctgggct acacctgcgc caacgacgtc acggcccgcg acctgcagcg gaccgacggg
     2041 cagtggactc gggcgaaggg cttcgacacg ttctgcccgc tggggccgtg gatctccacg
     2101 gacctggacc tggccgacgc ggcggtgcgc tgccgcgtga acggccagac gcgccaggac
     2161 ggttcgacgc gcgacctggt gttctccatc ccgcagctgg tgagccacct gtcggcgttc
     2221 acgacgctgc tgcccggcga cgtgatcctc acgggcacgc cggccggcgt gggcgagctg
     2281 cgcgacggcg acaccgtgga ggtcgaggtc ggcggcatcg gcgtgctgcg caaccccgtc
     2341 cgcgccgagg cgtagccccc gcctttccca acctggaacg ttcgtccggt gtttcggacg
     2401 aacggttcaa cccttcgcga gctccggcct gtaggcggcg atggcggcca gcatgtccgc
     2461 gcgcgtgcgg ccgcccctga cgcggagctt cacggtgcgg ccatcggcca gcaccgcccg
     2521 caaatccttc tcgcccagcg gctccaccgc caccacgtcc tcccacgggt ggatctcgcc
     2581 ggagtggtgc acccccagct cgctcaccgg cagcggcccg tcgaagaaca tcacgggcag
     2641 gtgcaccagc agcgtcacca gcgaccagcc gacgaacacc gccgagggca gcggcgcctt
     2701 ccgcgtggcg gcccagtacg ccaggaacgg caacaccacc atggccgcgg cgcggaacca
     2761 gttcgggacg cgctcggtgg cgcggaggag ggcgttcatc tggggctcta gggtatgcgc
     2821 aacatcagcc gcatccgctg cgcccgttgc ccctcccgag gtacccatga ccgcaccacg
     2881 cgtccgcttc tgccccgcgc ccagcggctg gctccacgtc ggtggcgccc gcacggcgct
     2941 gtacaactgg ctgtacgcgc gccaggcggg cggcgccttc atcctgcgca tcgaggacac
     3001 cgacgccgac cgcgccacgc gcgagtcggc cgaggcgatg atggagggcc tgcgcttcct
     3061 gggcctcgat tgggacgagg gccccggcca caccggcgcg cacggcccct acttccagtc
     3121 ggagcgcacc gacctgtacc aggcggtcgc gcacgcgctg ctgcaggccg gccacgtcta
     3181 ccccgccttc gagaccgagg aggaactcaa ggcgcagcgc gagcaggcca tgaaggaggg
     3241 ccgtcccccc ggctactcgg gcgcccatcg cgacctcacg caggagcaga tcgacgcctt
     3301 cgtggcggag gggcgcacgc cggtccttcg cgtgcgcacg ccggacgcgg gcgtgatcgg
     3361 cttcgacgac gcggtgcgcg gccaggtcga gttcgactgg aaggacatcc ccgacttcgt
     3421 ggtgcagcgc gccgacggca gcaccgtgta cttcctggcc aacgcggtgg acgacctggc
     3481 catgggcgtg aacttcgtcg cccgcggcga ggacctgctg tcggccacgc cgcgccagct
     3541 cctgctctat gagctgctca cccacgacgg cctgatcgac cgcgtgctcg ccgacctggg
     3601 ctaccccgcc cggccggacg gcgcgatggt gcccccgcgc ttcgcgcacc tgccgctgct
     3661 ggtgggcgag gaccgcaagc cgctgtccaa gcgccacggg tcggtggcca tcgaggagtt
     3721 ccgccgccag ggcttcctgc ccgaggtgct gctcaacttc ctggcgctgt gcgggtggag
     3781 ctacgagggc gtgcgggagc gcttcacggt cgacgagctg gtggagaagt tca
//
LOCUS       DATMKC010000102         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_64362, whole genome shotgun sequence.
ACCESSION   DATMKC010000102 DATMKC010000000
VERSION     DATMKC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64362"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1109)
                     /gene="galE"
                     /locus_tag="VNU01_03035"
     CDS             complement(147..1109)
                     /gene="galE"
                     /locus_tag="VNU01_03035"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWH31625.1"
                     /translation="MKLLVTGGAGYIGSVCAAHLLACGHEVVVLDDLSTGHADAVPEG
                     ARFVQAELGAAAERVLAEGFDGVLHFAARSLVGESVERPELYWWGNVVTSLRLLEAMR
                     AHDTPRLVFSSTAATYGEPAEIPIREDAPTRPTNPYGASKLAIDHAISSYAAAHGLAA
                     TSLRYFNVAGAHGPFGERHAVETHLIPIVLQVAAGTREAVQVFGEDWPTPDGTCVRDY
                     IHVHDLATAHLLALEHARPGHHAVYNLGNGTGFSVRQVLEACREVTGHPVPAVSAPRR
                     AGDPAVLVASSAAATAELGWRPERTSLTTIVADAWRFANAPVHI"
     gene            complement(1146..1868)
                     /locus_tag="VNU01_03040"
     CDS             complement(1146..1868)
                     /locus_tag="VNU01_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWH31626.1"
                     /translation="MNELVDTTEMYLRTIYELEEEGIVPLRARIAERLGQSGPTVSQT
                     VARMERDGLVVVAGDRHLELTDRGRGLAVAVMRKHRLAERLLVDVIGLEWEYVHAEAC
                     RWEHVMSDAVERKLVALLGNPTISPYGNPIPGLDELRRRPEEDRPGIAAPPSLEVGLQ
                     RLDEFARRGGGRVEIRRIAEHVQADHDLMGELKGAGVVPGHDVEVRAIPRFGDAVPVV
                     SGDGSESTVAPLVAHAVLVRAR"
BASE COUNT          272 a          807 c          765 g          280 t
ORIGIN      
        1 gcacgccgtc gaacccttcg gcgagcaccc gctccgccgc cgcgccgagt tcggcctgca
       61 cgaaccgggc gccctccggc acggttgcgg gagtgcctgc tcagcgcccg cccggacgag
      121 cccggcgacc cgctgtgagc gcgctgtcag atgtgcacgg gcgcgttggc gaaccgccac
      181 gcatcggcca cgatcgtcgt gagcgacgtg cgttcggggc gccagccgag ctcggcggtg
      241 gcggcggcgc tcgaggcgac gagtacggcg gggtcgccgg cccggcgtgg ggccgacacg
      301 gccggcacgg gatgccccgt gacctcccgg caggcctcca gcacctggcg cacggagaag
      361 ccggtgccgt tgcccaggtt gtacacggcg tggtggcccg ggcgggcgtg ctcgagcgcg
      421 agcaggtgtg ccgtggccag gtcgtgcacg tggatgtagt cgcgcacgca ggtgccgtcc
      481 ggggtcggcc agtcctcgcc gaacacctgc accgcctcgc gggtgccggc ggcgacctgc
      541 aggacgatcg ggatcaggtg cgtctccacc gcgtggcgct cgccgaacgg tccgtgggcg
      601 ccggcgacgt tgaagtagcg caggctcgtg gcggccaggc cgtgggcagc ggcgtaggac
      661 gagatcgcgt ggtcgatggc gagcttgctc gcgccgtagg ggttcgtcgg ccgggtgggc
      721 gcgtcctcgc ggatcgggat ctcggcgggc tcgccgtagg tggccgcggt ggaggagaac
      781 accagccgcg gggtgtcatg ggcccgcatg gcctccagca ggcgcaggga ggtcacgacg
      841 ttgccccacc agtacagctc cgggcgctcc accgactcgc cgaccagcga acgggcggcg
      901 aagtgcagca cgccgtcgaa cccttcggcg agcacccgct ccgccgccgc gccgagttcg
      961 gcctgcacga accgggcgcc ctccggcacg gcgtcggcgt ggccggtgga caggtcgtcg
     1021 agaaccacca cctcgtggcc gcaggccagc aggtgtgccg cgcagacgct tccgatgtag
     1081 cccgccccgc cggtgaccag gagtttcacg tgcctcgggc tccttcgggc cgcggggccg
     1141 cccggtcagc gggcacggac cagcaccgcg tgggccacca gcggggcgac ggtggactcg
     1201 ctgccgtccc cggacaccac cggcacggcg tcgccgaagc gggggatcgc ccgcacctcc
     1261 acgtcgtgcc ccggcaccac gccggcgccc ttgagctcgc ccatcaggtc gtggtcggcc
     1321 tgcacgtgct cggcgatccg acggatctcc acgcgcccgc cgccccggcg ggcgaactcg
     1381 tccaggcgct gcaggccgac ctccagcgac ggcggtgcgg cgatgcccgg gcggtcctcc
     1441 tccggccggc gccgcagctc gtccaggccc gggatcgggt ttccgtaggg ggagatggtc
     1501 gggttgccca gcagcgccac cagcttgcgt tccacggcat cgctcatcac gtgctcccac
     1561 cggcaggcct cggcgtgcac gtactcccac tccaggccga tgacgtccac cagaaggcgc
     1621 tcggccagcc ggtgcttgcg catcacggcg accgccaggc cacggccacg gtcggtgagc
     1681 tcgaggtgcc ggtcacccgc cacgacgacg agcccgtcgc gctccatccg ggccaccgtc
     1741 tgactcacgg tcgggccgct ctggccgagc cgctcggcga tccgcgcccg cagcgggacg
     1801 atgccctcct cctcgagttc gtagatcgtc cgcagataca tctcggtggt gtcgacaagc
     1861 tcgttcacgg cgggcccctt cgtctcggtc catcctatcc gggcaacgcg ttccggctcc
     1921 gaatacgtcc cgggacccgc ggccggctgc acagcacccg taccgccact gaccacggcc
     1981 gatccggcgc ccgcgggccg cggcgacgcc gcggatcgcc tacggtcgct ggcatgaacc
     2041 ccggcgccgc ggtggtctgg accccggact tcctcagcta ccagctctcc gacgaccacc
     2101 cgctcaaccc ggtccggctg gacc
//
LOCUS       DATMKC010000103         7837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_302105, whole genome shotgun sequence.
ACCESSION   DATMKC010000103 DATMKC010000000
VERSION     DATMKC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302105"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VNU01_03045"
     CDS             complement(<1..822)
                     /locus_tag="VNU01_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31627.1"
                     /translation="MRPFILHTHFYQPERLNPWTGALDPEPSAAPDRDWNARILRECY
                     APNGSARVFDQRRRVERIVNNYERVSFNFGPTLLSWMEQAAPRTYAKVLDGDFRSAVR
                     LGHGNAMAQAYNHMILPLANARDQRTQILWGMADFRHRFRRDPEGMWLPEAAIDQGTV
                     DALIDAGVRFTVLAPSQAGRVQEPDGGWRDANGDLDTSVPYRLRHSDDSGRWVAAFFY
                     DGELAQGLAFDPATVEAAVLVERLRAAGGDGLVHAALDGETFGHHHAFGELGLAYT"
     gene            complement(822..2672)
                     /gene="treZ"
                     /locus_tag="VNU01_03050"
     CDS             complement(822..2672)
                     /gene="treZ"
                     /locus_tag="VNU01_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWH31628.1"
                     /translation="MHQRPHRHGPELHGDGTATFRLWAPRAERVTLELRDGSHEMAAD
                     AGGWHTLRASALPGDRYGFTLPDGKTRPDPASRRQPDGVHELSAVADLGALPFGVERW
                     DAAPLERTVIYELHVGAFTPEGTFAAAAGRLDHLAALGVTHVEVMPVNGVNGTWNWGY
                     DGVAWYTVHEPYGGPDGFAAFVDACHARGLGVILDVVYNHLGPSGNFLPDFAPYLTQG
                     RSTWGDLLNLDGPDSDPVRDFIVGNALQWLRDYRCDGLRLDAVHALLDSSARHILAEL
                     ADAVDALEDELGRPLVLIAESDRNDPLTITRRAEHGLGMRAQWVDDLHHAIHTAVTGE
                     RDGYYVDYDGLPDVARAYRSGFLYDGRYSVYRQRTVGAPLPDTVPGHRLVTCVQNHDQ
                     VGNRAAGERLTTLVPPELVRVAIVLLLASPTTPMLWMGEEYGETNPFQFFTSHPEQEL
                     GRAVSEGRREEFADFSSWSAADVPDPQDPETFRRSTLDWARLDSPGGRERLALWRDLL
                     RLRRDVPALGEGRRDLVRIHRADADVLVAERRGPGGAPDGGAVLVCANIGEEPAAVPL
                     PGEGWSLLLDSEDRAYGGANRATVVGSTLTMGPGAAALLARRALEAPRGP"
     gene            complement(2689..4950)
                     /gene="glgB"
                     /locus_tag="VNU01_03055"
     CDS             complement(2689..4950)
                     /gene="glgB"
                     /locus_tag="VNU01_03055"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWH31629.1"
                     /translation="MRPSRRGTPEADRKPRDGGTDAPPRVQWRARPDEVEALLHGRHS
                     DPHRLLGAHPTGGAEHDGRAVGAVVRTHRPDAERVEVLVAGREPVEAERTAPGFFEAP
                     LPQQPQPDEYRLRVTYPAGSAFELRDPYAFWPTLGAIDLHLAGEGRHEELWRRMGAHV
                     IEVSGVRGTAFAVWAPNARSVRVVGDFNTWDGRLHPMRLLGSSGIWELFLPDVEPGAR
                     YKYEIVTADGSLVTRADPFAFWAEVPPGNASRIYSSEFAWTDDEWLGAREARQGVAAP
                     MSIYEVHLGSWRPGLDYRELAEQLADHVTALGFTHVELMPVAEHPFGGSWGYQVTGYY
                     APTSRFGTPDDFRWFVDRLHARGVGVIVDWVPAHFPKDEWALAAFDGTNLYEHMDPRK
                     GEHPDWGTLVFNFGRNEVRNFLIANALFWVEELHVDGLRVDAVASMLYLDYSRREGEW
                     VPNAFGGRENLEAVEFMMELNRTVYGRNPGVVTIAEESTAWPGVSRPAHLGGLGFGFK
                     WNMGWMHDTLAYFSRDPIHRRYHHNELTFGLMYAWSENFVLPLSHDEVVHGKRSLLDK
                     MPGDRWQKFANLRALYGWMWAHPGKQLLFMGGEFGQWREWSEERSLDWHLLEEDDHAG
                     LQRLVGDLNAVYRDQPALWARDADPAGFEWIDANDADHNFLAFTRRDADGAPLVCLAN
                     LSPVPRAGARVGMPAPGRWREVLNTDAEVYGGSNVGNLGGVEAEPAEWHGQPASAVVT
                     LPPLAVVWLAPEA"
     gene            complement(4956..6377)
                     /locus_tag="VNU01_03060"
     CDS             complement(4956..6377)
                     /locus_tag="VNU01_03060"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31630.1"
                     /translation="MSVLDALAAPSGERIVAGALRAWLPRQRWYAGKARDLEELHVHD
                     LAVLAGGPQPLLLVLVDVRYADGGTERYHVPLALEDGEADGADVVARLPEAALVDAGA
                     SAEACRLLVRLCLDDHAHGTSRGARLSGGALTAWRPADDAAVRRMGGEQSNTSVVLGD
                     AVLKVFRKLEAGENPDVELTRALTAGGTRTVPVQHGALLLQEREPTVLAVVSELVAPA
                     HEGWELAVGEVETLAEGRPRPQPGLLDDLDALGAAVAATHEGLARAFGRHDADTALLR
                     RWSAGMRAQALRVLDLAAERAPVECAAVLDRREELLEACAAGLEDPAAVGPVIRVHGD
                     LHLGQVLRGPDGWKLLDFEGEPARPLPERREPHTPLKDVAGMLRSFDYAAARVPPGSQ
                     VENWRATARRRFLAGYRATAAPAGLLPASEADGGRLLALMELDKATYELGYELANRPD
                     WVRVPVEGILTALDRRDDPTQEW"
     gene            complement(6374..>7837)
                     /gene="treS"
                     /locus_tag="VNU01_03065"
     CDS             complement(6374..>7837)
                     /gene="treS"
                     /locus_tag="VNU01_03065"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWH31631.1"
                     /translation="GYDIADYYTLLPEYGSVDDVAELLEAAHARGIRVIVDMVVNHTS
                     DAHPWFQEARDRDSDKHDWYVWSDTDDRWPDARIIFVDTERSNWTWDPVAERYYWHRF
                     FSHQPDLNYDNPEVREAMLEVIRFWLRLGFDGLRLDAVPYLFERDGTNCENLPETHAF
                     LRRIRAMVDEEFDDRVLLAEANQWPEDVVDYFGDADECHMAFHFPVMPRMFMAVRREE
                     AKPIVEILTNTPAIPDGCQWGIFLRNHDELTLEMVTDEERDYMYKEYARDPRMKMNVG
                     IRRRLAPLLDNSRRQIELFHALLLSLPGTPVMYYGDEIGMGDNIYLGDRDGVRTPMQW
                     TPDRNAGFSRADFAQLYLPVIMDPVYGYEALNVEQQQRNPNSLLHWVRDMVHIRKAHP
                     VFGTGSFRALDASNPKVLAFLRQGGAPGSGETILCVSNLASSAQPVELDLREFDGCMP
                     VELLGGTPFPPIGELPYFLTLAPHGFYWFELTAEARG"
BASE COUNT         1012 a         2876 c         2866 g         1083 t
ORIGIN      
        1 cgtgtacgcc aggcccagct ccccgaaggc gtgatggtgg ccgaaggtct ctccgtccag
       61 cgccgcgtgc accagcccgt cgccgccggc cgcgcgcagc cgctccacga gcacggcggc
      121 ctccaccgtg gccgggtcga acgccagccc ctgggccagc tcgccgtcgt agaagaacgc
      181 cgcgacccac cggccggagt cgtcgctgtg gcgcagccgg tagggcacgg aggtgtccag
      241 gtcgccgttc gcgtcgcgcc agcctccgtc gggctcctgc acgcggccgg cctgcgaggg
      301 cgccagcacg gtgaagcgca cgccggcgtc gatcagcgcg tccacggtgc cctggtcgat
      361 cgccgcctcc ggcagccaca tgccctcggg gtcgcggcgg aagcggtgcc ggaagtcggc
      421 catgccccac aggatctggg tgcgctggtc gcgcgcgttg gccagcggca ggatcatgtg
      481 gttgtaggcc tgcgccatcg cgttgccgtg gcccaggcgc acggcgctgc ggaagtcgcc
      541 gtcgagcacc ttggcgtagg tgcgcggcgc cgcctgctcc atccacgaca gcagcgtcgg
      601 gccgaagttg aacgacacgc gctcgtagtt gttgacgatg cgctcgaccc tgcggcgctg
      661 gtcgaacacg cgcgccgagc cgttgggcgc gtagcactcg cgcaggatgc gcgcgttcca
      721 gtcccggtcc ggggcggcgg agggctcggg gtccagcgcg cccgtccagg ggttcagccg
      781 ctccggctga tagaagtgcg tgtgcaggat gaacggtctc atcagggtcc tctgggtgcc
      841 tccagggcgc gtcgcgccag cagggccgcg gcccctggcc ccatcgtcag ggtggagccc
      901 accaccgtag cgcggttcgc gccgccgtag gcccgatcct ccgagtccag caggaggctc
      961 cagccctccc cgggcagcgg caccgccgcc ggctcctcgc cgatgttggc gcacaccagc
     1021 accgcgccgc catccggtgc gccgcctggg ccgcgccgct cggccaccag cacgtccgcg
     1081 tcggcgcggt ggatccgcac gaggtcgcgg cggccctcgc ccagcgccgg cacgtcgcgc
     1141 cgcagccgca gcaggtcccg ccacagggcc agccgctccc ggccgccggg gctgtccagc
     1201 cgtgcccagt ccagggtgga gcgccggaac gtctcggggt cctgcgggtc gggcacgtcg
     1261 gcggccgacc aggaggagaa gtcggcgaac tcctcgcgcc ggccctcgct cacggcccgc
     1321 cccagttcct gctcgggatg ggaggtgaag aactggaagg ggttggtctc gccgtactcc
     1381 tcgcccatcc acagcatcgg cgtcgtgggt gaggccagca gcagcacgat cgccacgcgc
     1441 accagctcgg gcggcaccag cgtggtcagc cgctcgcccg ccgcgcggtt gcccacctgg
     1501 tcgtggttct ggacgcacgt caccaggcgg tgtcccggga cggtgtcggg cagcggcgcg
     1561 cccacggtgc gctgccggta gaccgagtag cgcccgtcgt ataggaaccc ggagcggtac
     1621 gcgcgcgcga cgtcgggcag gccgtcgtag tcgacgtagt agccgtcgcg ctcgcccgtg
     1681 accgccgtgt ggatggcgtg gtgcaggtcg tcgacccact gcgcccgcat gcccaggccg
     1741 tgctcggcgc gccgggtgat cgtgagcgga tcgttgcggt cggactcggc gatcaggacc
     1801 agcggcctgc ccagctcgtc ctccagcgcg tccaccgcgt cggccagctc cgccaggatg
     1861 tggcgggcgg aggagtccag cagggcgtgt accgcgtcca gccgcaggcc gtcgcagcgg
     1921 tagtcgcgca gccactgcag cgcgttgccc acgatgaagt cgcgcaccgg gtcgctgtcg
     1981 gggccgtcca ggttgagcag gtcgccccag gtgctgcggc cctgtgtgag gtagggcgcg
     2041 aagtccggca ggaagttgcc gctgggcccg aggtggttgt agacgacgtc caggatcacg
     2101 ccgagcccgc gcgcgtggca ggcgtcgacg aacgcggcga agccatccgg gccgccgtag
     2161 ggctcgtgca ccgtgtacca ggcgacgccg tcgtagcccc agttccaggt cccgttgacg
     2221 ccgttgaccg gcatcacctc cacgtgggtg acccccagtg ccgccaggtg gtccagccgc
     2281 ccggcggccg ccgcgaaggt cccctccggc gtgaacgcgc cgacgtgcag ctcgtagatg
     2341 acggtgcgct ccagcggcgc ggcgtcccag cgctcgacgc cgaagggcag cgcgccgagg
     2401 tccgcgaccg cggacagctc gtgcacgcca tcgggttggc gccgcgacgc gggatccgga
     2461 cgggtcttgc cgtccgggag cgtgaagccg tagcggtcgc caggcagcgc cgaggcgcgc
     2521 agggtgtgcc agccgcccgc gtcggccgcc atctcgtggg acccgtcgcg cagctccagc
     2581 gtcacccgct cggcgcgtgg cgcccacagc cggaacgtcg ccgtgccgtc gccgtggagc
     2641 tcgggtccat ggcgatgggg gcgctggtgc acgggacgcg ctcctgtctc aggcctcggg
     2701 cgccagccag acgacggcaa ggggcggaag ggtcaccacg gcggacgcgg gctggccgtg
     2761 ccactcggcg ggctctgcct ccacgccgcc gaggttgccg acgttgctgc cgccgtagac
     2821 ctcggcgtcg gtgttgagca cctcgcgcca gcggccgggc gccggcatgc ccacgcgggc
     2881 gccggcccgc ggcaccggcg acaggttcgc caggcacacc agcggcgcgc catccgcgtc
     2941 gcggcgcgtg aacgccagga agttgtggtc ggcgtcgttc gcgtcgatcc actcgaatcc
     3001 cgccgggtcg gcgtcgcgcg cccacagcgc gggctggtcg cggtacaccg cgttgaggtc
     3061 gccgaccagg cgctgcaggc ccgcgtggtc gtcctcctcc agcaggtgcc agtccagcga
     3121 gcgctcctcg ctccactcgc gccactgccc gaactccccg cccatgaaca gcagctgctt
     3181 gccggggtgc gcccacatcc agccgtacag cgcgcgcagg ttcgcgaact tctgccagcg
     3241 gtcgccgggc atcttgtcga gcaggctgcg cttgccgtgc acgacctcgt cgtgcgacag
     3301 cggcagcacg aagttctcgc tccaggcgta catcagcccg aaggtgagct cgttgtggtg
     3361 gtagcggcgg tggatcgggt cgcgcgagaa gtacgccagg gtgtcgtgca tccagcccat
     3421 gttccacttg aagccgaacc ccaggccgcc caggtgcgcc ggccgcgaca cgcccggcca
     3481 ggcggtggac tcctcggcga tggtgaccac gcccgggttg cgcccgtaca cggtgcggtt
     3541 gagttccatc atgaactcga ccgcctccag gttctcgcgg ccgccgaagg cgttgggcac
     3601 ccactcgccc tcccgccgcg agtagtccag gtacagcatc gaggcgacgg cgtccacgcg
     3661 caggccgtcg acgtgcagct cctccaccca gaacagggcg ttggcgatca ggaagttgcg
     3721 cacctcgttg cgcccgaagt tgaacaccag cgtgccccag tccgggtgct cgcccttgcg
     3781 cgggtccatg tgctcgtaca ggttcgtgcc gtcgaaggcg gccagcgccc actcgtcctt
     3841 ggggaagtgc gcgggcaccc agtccacgat cacgccgacg ccgcgcgcgt gcaggcggtc
     3901 gacgaaccag cggaagtcgt ccggggtgcc gaagcgcgac gtgggcgcgt agtacccggt
     3961 gacctggtag ccccaggagc cgccgaaggg gtgctccgcc acgggcatca gctcgacgtg
     4021 cgtgaagccg agcgcggtga cgtggtcggc gagctgctcg gccagctccc ggtagtccag
     4081 gcccggccgc caggagccca ggtggacctc gtagatcgac atgggggcgg ccacgccctg
     4141 gcgcgcctcc cgggcgccga gccactcgtc gtcggtccag gcgaactcgg aggagtagat
     4201 gcggctggcg ttgccgggcg ggacctcggc ccagaacgcg aacgggtccg cgcgcgtcac
     4261 cagcgagccg tcggcggtga cgatctcgta cttgtagcgg gcgcccggct cgacgtcggg
     4321 caggaacagc tcccagatgc cggaggagcc cagcaggcgc atggggtgga ggcggccgtc
     4381 ccaggtgttg aagtcgccga ccacccgcac gctcctggcg ttgggggccc acacggcgaa
     4441 ggcggtgccg cgcacgccgc tcacctcgat gacgtgcgcg cccatgcgcc gccacagctc
     4501 ctcgtggcga ccctcgccgg ccaggtgcag gtcgatcgct cccagcgtgg gccagaaggc
     4561 gtaggggtcg cgcagctcga aggcggagcc cgccgggtag gtcacccgca gccggtactc
     4621 gtccggctgc ggctgctgcg gcagcggcgc ctcgaagaag ccgggcgcgg tgcgctcggc
     4681 ctcgacgggc tcgcgcccgg ccaccagcac ctcgacgcgc tcggcgtcgg gccggtgcgt
     4741 gcggaccacc gcgccgacgg cgcggccgtc gtgctcggcg ccgccggtgg ggtgcgcgcc
     4801 gagcaggcgg tgcgggtcgc tgtggcgtcc gtgcagcagc gcctccacct cgtccggccg
     4861 tgcccgccac tggacgcgcg gcggcgcgtc cgtgccgccg tcgcgcggct tgcggtcggc
     4921 ttccggggtg ccgcgtcggc tcgggcgcat gcgtttcacc actcctgtgt cggatcgtct
     4981 cgtcggtcca gtgcggtcag gattccctcc acgggcactc tcacccagtc cggacggttg
     5041 gccagctcgt agcccagctc gtaggtcgcc ttgtccagct ccatgagcgc cagcagcctg
     5101 ccgccgtccg cctccgaggc cggcagcagc ccggccgggg cggcggtcgc gcggtagccg
     5161 gccaggaagc gccggcgggc cgtggcgcgc cagttctcca cctggctgcc cggcggcacg
     5221 cgggccgcgg cgtagtcgaa cgagcgcagc atcccggcga cgtccttgag cggggtgtgc
     5281 ggctcgcgcc gttccggcag cggccgggcc ggctcccctt cgaagtccag cagcttccac
     5341 ccgtccgggc cgcgcagcac ctggcccagg tgcaggtcgc cgtgcacgcg gatgaccggg
     5401 cccacggcgg ccgggtcctc aaggcccgcg gcgcacgcct ccagcagctc ctcgcggcgg
     5461 tccagcaccg cggcgcactc cacgggcgcg cgctcggcgg ccaggtccag gacgcgcagc
     5521 gcctgtgccc gcatccccgc ggaccatcgc cgcagcagcg cggtgtcggc gtcgtggcgg
     5581 ccgaacgcgc gcgccaggcc ctcgtgcgtc gcggcgaccg ccgcgcccag cgcgtcgagg
     5641 tcgtccagca gccccggctg gggccgcggc cgcccctcgg ccagcgtctc gacctcgccg
     5701 acggccagct cccagccctc gtgcgccggc gcgaccagct cgctcaccac ggccaggacc
     5761 gtcggctcgc gctcctgcag cagcagggcg ccgtgctgga ccggcacggt gcgggtgccg
     5821 ccggcggtca gggccctggt gagttccaca tcggggttct ccccggcttc cagcttgcgg
     5881 aacaccttga ggaccgcgtc gcccagcacc accgacgtgt tggactgctc gccgcccatg
     5941 cggcgcacgg ccgcgtcgtc cgccggccgc cacgcggtga gcgcgccgcc ggacaggcgg
     6001 gccccccggc tggtgccgtg cgcgtggtcg tccaggcaca ggcgcaccag cagccggcac
     6061 gcctccgccg aggcgcccgc gtcgacgagg gcggcctcgg gcagccgcgc cacgacgtcg
     6121 gcgccgtcgg cctcgccgtc ctccagcgcg agcggcacgt ggtagcgctc ggtcccgccg
     6181 tcggcgtagc gcacgtccac gagcaccagc agcagcggct gtggcccgcc ggccagcacg
     6241 gccaggtcgt ggacgtgcag ctcctccagg tcgcgcgcct tgcccgcgta ccagcgctgc
     6301 cgtggcagcc acgcccgcag cgcgcctgcg acgatgcgct cgccggacgg cgccgccagg
     6361 gcgtcgagca cgctcatccc cgcgcctcgg cggtcagctc gaaccagtag aagccgtgcg
     6421 gcgcgagcgt caggaagtac ggcagctcgc cgatgggcgg gaacggggtg ccgcccagca
     6481 gctccaccgg catgcagccg tcgaactcgc gcaggtccag ctcgaccggc tgggcgctgg
     6541 aggcgaggtt gctgacgcac aggatggtct cgccggagcc cggtgcgccc ccttggcgca
     6601 ggaacgcgag caccttgggg ttggaggcgt ccagggcgcg gaacgagccg gtgccgaaga
     6661 ccggatgcgc cttgcggatg tgcaccatgt cgcgcaccca gtgcagcagc gagttcgggt
     6721 tgcgctgctg ctgctccacg ttgagcgcct cgtagccgta gacggggtcc atgatcaccg
     6781 gcaggtacag ctgggcgaag tcggcgcggc tgaagcccgc gttgcggtcg ggcgtccact
     6841 gcatgggcgt gcggacgccg tcgcggtcac ccaggtagat gttgtcgccc atgccgatct
     6901 cgtcgccgta gtacatgacc ggcgtgcccg gcaggctcag cagcagcgcg tggaacagct
     6961 cgatctggcg gcgggagttg tccagcagcg gcgccaggcg gcggcggatg cccacgttca
     7021 tcttcatgcg ggggtcgcgc gcgtactcct tgtacatgta gtcgcgctcc tcgtcggtga
     7081 ccatctccag ggtcagctcg tcgtggttgc gcaggaagat gccccactgg cagccgtcgg
     7141 ggatcgccgg ggtgttggtg aggatctcca cgatcggctt ggcctcctcg cgccgcaccg
     7201 ccatgaacat gcgcggcatg acggggaagt ggaacgccat gtggcactcg tcggcgtcgc
     7261 cgaagtagtc cacgacgtcc tccggccact ggttcgcctc ggccagcagc acgcggtcgt
     7321 cgaactcctc gtccaccatg gcgcggatgc gccgcaggaa cgcgtgcgtt tccggcaggt
     7381 tctcgcagtt ggtcccgtcg cgctcgaaca ggtacggcac cgcgtccagg cgcagcccgt
     7441 cgaagcccag gcgcagccag aagcggatga cctccagcat cgcctcgcgg acctcggggt
     7501 tgtcgtagtt gaggtccggc tggtggctga agaagcggtg ccagtagtag cgctcggcca
     7561 ccgggtccca ggtccagttg gagcgctcgg tgtccacgaa gatgatgcgc gcgtcgggcc
     7621 agcggtcgtc ggtgtcggac cacacgtacc agtcgtgctt gtccgagtcg cggtcgcgcg
     7681 cctcctggaa ccacgggtgg gcgtcgctgg tgtggttgac caccatgtcc acgatcacgc
     7741 ggatgccgcg cgcgtgcgcg gcctcgagca gctccgccac gtcgtcgacg ctgccgtact
     7801 ccggcagcag cgtgtagtag tcggcgatgt catagcc
//
LOCUS       DATMKC010000104         4582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_348341, whole genome shotgun sequence.
ACCESSION   DATMKC010000104 DATMKC010000000
VERSION     DATMKC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348341"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VNU01_03070"
     CDS             complement(<1..335)
                     /locus_tag="VNU01_03070"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH31632.1"
                     /translation="MAEPLGYQFTVDCADPAGLAAFWAQALGFEPDAHHSGQDGRYAA
                     IRDPRRRPGGRIVFQRVAEPKTVKNRAHLDVLCGGERRAEQVARLVALGASVLGEVDE
                     AAGRWTVLA"
     gene            complement(365..1291)
                     /locus_tag="VNU01_03075"
     CDS             complement(365..1291)
                     /locus_tag="VNU01_03075"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWH31633.1"
                     /translation="MPITEVSKIWMDGELVDWADATVHILTPTLHYGWGVFEGVRAYA
                     TDRGPAVFRHREHMERLHRSAEILQMDIPFSVDELMEGTREVIRAAGVESCYIRPLAY
                     LGYGEMGLNPLPSKVSVCIAIWPWGTYLGDEGLLNGIRAKVSSWTRIGANMIPTGAKA
                     CGVYINSSLAKVEALKAGYDEAILLNEHGRVAEGSGENIFVVRDGVLMTPALSEGVLD
                     GITRASVIQFAQDLDIPFVEANLLRQDLYTADEAFFTGTAAEVVPIRSVDEREIGAPG
                     PVTKQIQDLFFDVVKGKSERYAGMLDYVDDRT"
     gene            complement(1298..2374)
                     /locus_tag="VNU01_03080"
     CDS             complement(1298..2374)
                     /locus_tag="VNU01_03080"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWH31634.1"
                     /translation="MEAAVGSYDLAVIGGDGVGPEVTAQALKAVRAAADRFGFSVETT
                     GYDLGGVRYLSTGEVLPETVIEELRRHDAILLGAVGTPDVPPGVLERGLLLKLRFAFD
                     QYVNWRPVKLYEGVPTPIAGLTPERCDLVVVRENTEGLYAGAGGSVYKGTPNEVATQE
                     SLNTRHGVERVVRFAFDQAQARRGKLTLCHKTNVLTFAGDLWQRTVDAVAADYPDVTT
                     DYVHVDAMCLYLVQSPERFDVVVTDNLFGDIVTDLGAAVQGGLGLAASGNLNPDKTAP
                     SMFEPVHGSAPDIAGKGWANPVAAVLSAAMCLAHLGERKAAEAVEQAAARQLTTLGAM
                     AGAAMGYSTDEIGDRIASEIAQGA"
     gene            complement(2409..2999)
                     /locus_tag="VNU01_03085"
     CDS             complement(2409..2999)
                     /locus_tag="VNU01_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWH31635.1"
                     /translation="MPTVLCLDLMGVVLHDPYLEAIEAAVEVPLAEVWRHKDATSWPE
                     FECGVIDEAEFERRFWADAGMAFDAEAFHRARRAGYRFMDGMEALLGDARAAGLELHV
                     ASNYPVWIEELRERFGLDELFDGVWASCHLGVRKPDPRFFERLLDKVGRPAAQCLFAD
                     DRPPNVEAAAAAGLRAHLFTSAADLRARLAAEGVPV"
     gene            3186..3692
                     /locus_tag="VNU01_03090"
     CDS             3186..3692
                     /locus_tag="VNU01_03090"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWH31636.1"
                     /translation="MTVLHGAATTLRPVQPGDAEALTRILAEPEVSRWWPRYDADRVQ
                     RELIDAEHTTVFAIEVEGQVVGSIQYVEEPSPDYRHASVDVFLDPLWHGKGLGTDAVR
                     TLARHLVHDRGHHRLAIDPAAENEKAIRTYKRVGFRPVGVMRSYERSADGEWQDCLLM
                     DLLKADLH"
     gene            3697..4086
                     /locus_tag="VNU01_03095"
     CDS             3697..4086
                     /locus_tag="VNU01_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH31637.1"
                     /translation="MHVVNSRVLLRPRDFDASVRFYSDAIGLVRFREWGAPGRRGVVF
                     FLGGGLLELTESAGASGEVGTVRLWLQVADAEAARAELEAKGVAVASPPERKPWGLVE
                     MTVHDPDGLPLVIVEVPDDHPLRKDTR"
     gene            complement(4091..>4582)
                     /locus_tag="VNU01_03100"
     CDS             complement(4091..>4582)
                     /locus_tag="VNU01_03100"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWH31638.1"
                     /translation="VYVDEPGVEVVVGERPSTHRLHLDYIGEGLGRACLSEVVRERPV
                     YAFSVLGAEDAPVRVAAAAVGHAGIPWVSVGTLFPGTHISVRPPGVSKWAGVLAWCAD
                     QGIDPAHVLAVGDGENDLELLEAARVSCVVSDGCEAALALADHVIEPAEHGGWRAILE
                     LLA"
BASE COUNT          645 a         1722 c         1595 g          620 t
ORIGIN      
        1 tcggcgagca cggtccagcg gccggcggcc tcgtcgacct cgccgagcac ggaggcgccc
       61 agcgccacca gccgcgcgac ctgctcggcg cggcgctcac cgccgcacag cacgtccaga
      121 tgggcccggt tcttgaccgt cttgggctcg gcgacccgct ggaacacgat gcgcccgccg
      181 gggcgccgcc gggggtcccg gatcgcggcg tagcgcccgt cctgcccgct gtggtgggcg
      241 tcgggctcga agccgagcgc ctgcgcccag aacgccgcga gtccggcggg gtcggcgcag
      301 tcgacggtga actggtagcc gagcggctca gccatcgcca cgcgccaccg tcttcacgga
      361 gccgtcaggt ccggtcgtcc acatagtcga gcatgccggc gtagcgctcg ctcttgccct
      421 tcaccacgtc gaagaacaga tcctggatct gcttggtcac cggcccgggc gcgccgatct
      481 cgcgctcgtc cacggagcgg atcggcacca cctcggccgc ggtgcccgtg aagaacgcct
      541 cgtcggcggt gtacaggtcc tgccgcagca ggttggcctc cacgaacggg atgtcgaggt
      601 cctgggcgaa ctggatgacg ctggcgcggg tgatgccgtc cagcacgccc tcgctcagcg
      661 ccggcgtcat cagcacgccg tctctgacca cgaagatgtt ctcgccggag ccctccgcga
      721 cgcggccgtg ctcgttgagc aggatggcct cgtcgtagcc ggccttcaac gcctccacct
      781 tggccaggga gctgttgatg tagacgccgc acgccttggc gccggtgggg atcatgttcg
      841 cgccgatgcg cgtccaggag ctgaccttgg cgcggatgcc gttgagcagg ccctcgtcgc
      901 ccaggtaggt gccccacggc cagatcgcga tgcacacgct caccttgctg ggcagtgggt
      961 tcaggcccat ctcgccgtag cccaggtagg ccagcggccg gatgtagcag ctctccaccc
     1021 ccgcggcgcg gatgacctcg cgcgtgccct ccatcagctc gtcgaccgag aacgggatgt
     1081 ccatctgcag gatctcggcc gagcggtgca ggcgctccat gtgctcacgg tggcggaaca
     1141 ccgccgggcc gcgatccgtg gcgtaggcgc gcacgccctc gaagacgccc cagccgtagt
     1201 gcagcgtcgg cgtgaggatg tggacggtgg cgtcggccca gtcgaccagc tcgccgtcca
     1261 tccagatctt ggagacctcg gtgatgggca tggcggttca ggctccttgc gcgatttcag
     1321 aggcgatgcg gtcgccgatc tcgtccgtgg agtatcccat ggccgctccc gccatggcac
     1381 ccagcgtcgt gagctggcgg gccgccgcct gctccaccgc ctcggcggcc ttgcgctccc
     1441 ccaggtgcgc caggcacatc gcggcgctca gcaccgcggc gaccgggttg gcccacccct
     1501 tgcccgcgat gtcgggcgcg gagccgtgca ccggctcgaa catgctgggc gcggtcttgt
     1561 ccgggttcag gttgccggag gcggccaggc ccaggccgcc ctgcaccgcg gcgcccaggt
     1621 ccgtgacgat gtcgccgaac aggttgtcgg tcaccaccac gtcgaaccgc tccggcgact
     1681 gcaccaggta caggcacatc gcgtcgacgt gcacgtagtc ggtggtcacg tccgggtagt
     1741 cggccgcgac cgcgtcgacg gtgcgctgcc acaggtcacc ggcgaaggtg agcacgttgg
     1801 tcttgtggca cagggtgagc ttgccgcgcc gggcctgcgc ctggtcgaac gcgaagcgca
     1861 ccacacgctc cacgccgtgg cgggtgttga gcgactcctg cgtggcgacc tcgttggggg
     1921 tgcccttgta gacgctgccg ccggcgccgg cgtacaggcc ctcggtgttc tcccgcacca
     1981 ccaccaggtc gcagcgctcc ggggtcaggc ccgcgatcgg cgtgggcacg ccctcgtaca
     2041 gcttcacggg acgccagttg acgtactggt cgaacgcgaa gcgcagcttc agcagcaggc
     2101 cgcgctccag cacgccgggc gggacgtcgg gggtgcccac ggcgcccagg aggatggcgt
     2161 cgtggcggcg cagctcctcg atcacggtct cgggcaggac ctcgccggtg gacaggtagc
     2221 gcaccccgcc caggtcgtag cccgtggtct ccacggagaa gccgaagcgg tccgcggcgg
     2281 cgcgcacggc cttcagggcc tgcgcggtca cctccggccc cacgccgtcc ccaccgatga
     2341 ccgcaaggtc gtagctgccc actgccgcct ccatgccgag tcccgaacgc caggcagtgt
     2401 acgggccgtc agacgggcac gccctcggcg gcgaggcggg cgcggaggtc ggcggcggac
     2461 gtgaacaggt gcgcgcgcag cccggcggcc gccgccgcct ccacgttcgg cgggcggtcg
     2521 tcggcgaaca ggcactgcgc cgccgggcgg cccaccttgt cgagcagccg ctcgaagaag
     2581 cgcgggtcgg gcttgcgcac ccccaggtgg cagctcgccc acacgccgtc gaacagctcg
     2641 tccaggccga agcgctcgcg cagctcctcg atccagacgg gatagttgct ggcgacgtgc
     2701 aactccagcc cggccgcgcg ggcgtcgccg agcagcgcct ccatgccgtc catgaaccgg
     2761 tagcccgcac ggcgggcgcg gtggaacgcc tccgcgtcga acgccatgcc cgcatcggcc
     2821 cagaagcgcc gctcgaactc cgcctcgtcg atgacgccgc actcgaactc cggccaggag
     2881 gtcgcgtcct tgtggcgcca gacctcggcc agcggcacct cgacggccgc ctcgatcgcc
     2941 tccaggtagg ggtcgtgcag gacgacaccc atcaggtcca ggcacagcac cgtcggcatg
     3001 gccccattgt gcccgcggcg aacccccgcg acggaacccg cgacgcggga cgcggcgtct
     3061 ggcaccatgg acgcatcgca cggccctgcc gccaccggcg ccggcaggcc ggccacgccg
     3121 gacgcgcccg tgcggcgcga tccgagccaa ggcacaacgg ggctctcccc aggccaggag
     3181 gacgaatgac cgtgttgcac ggcgccgcta ccacgctccg cccggttcag ccgggggacg
     3241 ccgaggcgct gacccgcatc ctggccgaac ccgaggtgtc ccgctggtgg ccccgctacg
     3301 acgccgaccg ggtgcagcgc gagctcatcg acgccgagca caccacggtg ttcgccatcg
     3361 aggtcgaagg gcaggtggtc ggctccatcc agtacgtcga ggagccgtcg ccggactacc
     3421 gccacgccag cgtcgacgtg ttcctggacc ccctgtggca cggcaagggc ctgggcaccg
     3481 acgcggtgcg cacgctggcc cgccacctcg tccacgaccg cgggcaccat cgcctggcga
     3541 tcgacccggc cgccgagaac gaaaaggcca tccggaccta caagcgggtg ggcttccggc
     3601 ccgtcggcgt catgcgctcc tacgagcgca gcgccgacgg cgaatggcag gactgcctgc
     3661 tcatggacct gctcaaggcc gacctgcact gacgccatgc acgtcgtgaa ctcgcgggtg
     3721 ctgctgcggc cgcgcgactt cgacgcctcg gtgcgcttct actccgacgc catcggcctg
     3781 gtgcgcttcc gcgagtgggg ggcgccgggc cggcgcggcg tggtgttctt cctgggcggc
     3841 ggcctgctgg agctcaccga gtccgccggc gccagtggcg aggtgggcac cgtgcggctg
     3901 tggctgcagg tcgccgacgc cgaggcggcg cgcgcggagc ttgaggccaa gggggtcgcg
     3961 gtggcctcgc cgccggagcg caagccgtgg ggcctggtgg agatgaccgt gcacgacccc
     4021 gacggcctgc cgctggtcat cgtggaagtc cccgacgatc acccattgcg aaaagacacg
     4081 cgctagcgcg ctaggcgagc agttccagga tcgcgcgcca gccgccgtgc tcggcaggct
     4141 cgatgacgtg gtcggccagc gcgagcgccg cctcgcagcc gtcggacacc acgcacgaca
     4201 cccgcgccgc ctccagcagc tccaagtcgt tctcgccgtc cccgaccgcc agcacgtggg
     4261 ccgggtcgat gccctggtcc gcgcaccacg cgagcacgcc cgcccacttg ctcacgcccg
     4321 gcgggcgcac gctgatgtgg gtccccggga acagcgtgcc cacgctcacc cacgggatgc
     4381 cggcatggcc cacggcggca gccgcgacgc gcaccggggc gtcctcggcg cccaacacgg
     4441 agaacgcgta caccgggcgc tcccgcacca cctcgctgag gcaggcccgg cccagcccct
     4501 ccccgatgta gtccaggtgc agccggtgcg tcgacgggcg ctcccccacc accacctcga
     4561 ccccaggctc gtccacgtac ac
//
LOCUS       DATMKC010000105         2913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_165089, whole genome shotgun sequence.
ACCESSION   DATMKC010000105 DATMKC010000000
VERSION     DATMKC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165089"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VNU01_03105"
     CDS             complement(<1..975)
                     /locus_tag="VNU01_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31639.1"
                     /translation="MSHVAFVLHSHLPWLRRNGTYPVGEEWLFQSWSESYLPVLALLE
                     RLAEDGHRDVLTLGVTPVLAEQMDDPYLLREFHGWLGRRLVDLEYTVSRYGAGDRAAL
                     GEVWEHHWRRQTALLDVVERRILSTGLTAPFRALADAGVIELLGGPATHPYLALADDP
                     ALIDAQIAGGLDVHERRFGRRPTGVWTPECAYRPAGTVADPTAPPVAVAADGTPTLSR
                     SATVLPGLEEFWEAAGVDHFVVDGPTLARAAGAPPRDWAHTGGELVPVGDPLDVLDHP
                     VLVGDSGVAAFGRNLAVSYAVWNPHGGYPAHPDYRDFYRIDLEGGFKSW"
     gene            complement(972..1754)
                     /locus_tag="VNU01_03110"
     CDS             complement(972..1754)
                     /locus_tag="VNU01_03110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWH31640.1"
                     /translation="MTHSLPLTGERTVPGIPHENYWFQRHVVAYRFARARIRGLAVMD
                     AGCGEGYGAAMLADAASAVTGVELVPDVVAHAKAAYPPAAHPGLSFVEADLCRLPMPD
                     ASFDAVVSFQVIEHLPDVGGYLDEIRRVLKPGGEFLCATPNRLTFTPGSDTPVNPFHV
                     KEFTAAELAECLGSRFRVKAVLGVHHGGRIKALERLARKPVTDLQFAAPPDEWPRWLR
                     TAIAAVQPRDFTLKAADVDASLDLVAVAQRPPTPKARVEPAA"
     gene            1898..2689
                     /locus_tag="VNU01_03115"
     CDS             1898..2689
                     /locus_tag="VNU01_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWH31641.1"
                     /translation="MRIICPVKRVPDTAADKKINPDDNTVNRDASEPVLNANDEWAIE
                     EAMRIKERTPGTEVVALCMGPDAAQSIVRKALSYGLDAAIQVTDPALHGSDAVATARV
                     LAKALEGETYDLIIMGNQSSDARSMLVPAMLAEFLGLPALTFCQKLDVDGTTFRAERQ
                     TSNGYQVVEAQGPVVVSVVEAINEPRYPSFKGIMAAKSKPLTTKSLGDLGVDGGEVGL
                     DGSRTQVIDVTPRPPKQAGEKVEDDGSGTVGAKALADFLAAKKFV"
     gene            2720..>2913
                     /locus_tag="VNU01_03120"
     CDS             2720..>2913
                     /locus_tag="VNU01_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31642.1"
                     /translation="MADILVFVDHDKGTVKKVSAQMLTAAKNKGGSVSAAIFGEGASG
                     AVERAGAFGASKAYVWEAPEA"
BASE COUNT          456 a         1057 c         1035 g          365 t
ORIGIN      
        1 ccacgacttg aagccgccct cgaggtcgat ccggtagaag tcgcggtagt cggggtgggc
       61 cgggtagccg ccgtgcgggt tccacaccgc gtagctgacc gcgaggttgc gcccgaacgc
      121 cgccacgccg ctgtcgccga cgagcacggg atggtccagc acgtccagcg ggtcgccgac
      181 gggcacgagc tctccgcccg tgtgcgccca gtcgcgcggc ggggcgccgg cggcgcgcgc
      241 cagggtcggc ccgtcgacca cgaaatggtc gaccccggcc gcctcccaga actcctccag
      301 gccgggcagc accgtggccg agcgcgacag cgtcggggtg ccgtcggcgg ccacggcgac
      361 cggcggcgcg gtcgggtcag ccaccgtgcc ggcgggccgg taggcgcact ccggcgtcca
      421 cacgccggtg gggcgccggc cgaagcggcg ctcgtggacg tccagcccgc ccgcgatctg
      481 cgcgtcgatc agcgcggggt cgtcggccag cgcgaggtac gggtgcgtcg ccgggccgcc
      541 cagcagctcg atcacgccgg cgtcggccag tgcccggaac ggggcggtga gccccgtgga
      601 caggatccgc cgctccacca cgtccagcag cgcggtctgc cgacgccagt ggtgctccca
      661 cacctcgccc agcgcggccc ggtcgccggc tccgtagcgc gacaccgtgt actccaggtc
      721 caccaggcgc cggcccagcc agccgtggaa ctcgcgcagc aggtaggggt cgtccatctg
      781 ctcggccagc accggggtca cgcccagcgt gagcacgtcg cgatgcccgt cctcggccag
      841 gcgctccagc agcgccagca ccggcaggta gctctccgac caggactgga acagccactc
      901 ctcgcccacg ggataggtcc cgttgcgccg cagccacggc aggtggctgt gcagcacgaa
      961 cgcgacgtga ctcatgccgc cggctccact cgtgccttcg gcgttggggg ccgctgggcc
     1021 acggccacca ggtccagcga cgcgtcgacg tcggcggcct tcagcgtgaa gtcgcgcggc
     1081 tgcaccgcgg cgattgcggt gcgcagccag cgcggccact cgtccggggg cgcggcgaac
     1141 tgcaggtccg tcacgggctt gcgcgccagg cgctccagcg ccttgatgcg cccaccgtgg
     1201 tggacgccca ggacggcctt cacccggaag cgcgagccca ggcattcggc cagctcggcg
     1261 gcggtgaact ccttgacgtg gaaggggttc accggtgtgt cgctgcccgg ggtgaacgtc
     1321 agccggttgg gtgtggcgca caaaaactcc ccaccgggct tgagcacgcg gcggatctcg
     1381 tccaggtagc cgccgacgtc cggcaggtgc tcgatgacct ggaagctgac caccgcgtcg
     1441 aaggaggcgt ccggcatggg caggcggcac aggtccgcct ccacgaagga caagccgggg
     1501 tgggccgccg ggggataggc cgccttcgcg tgcgccacca cgtcggggac cagctccacg
     1561 cccgtcaccg ccgacgccgc gtccgcgagc atcgccgcgc cgtagccctc gccgcagccg
     1621 gcgtccatga cggccagtcc gcggatgcgc gcccgcgcga agcgataggc cacgacatgc
     1681 cgctggaacc agtagttctc gtgcgggatg ccgggcacgg tgcgctcgcc cgtcagggga
     1741 agagagtggg tcatcaacag tccggatggt ctggcaacgg ggccttgccg atttgacgca
     1801 cgatattacc gcgcggtaac taatgagccg aagactggac cacgtccctg ggctctcgcc
     1861 ctcccgccac cacccgccga cctcgacgga ggaccctgtg aggatcatct gcccggtcaa
     1921 gcgcgtgccg gacacggccg cggacaagaa gatcaacccg gacgacaaca cggtcaaccg
     1981 tgacgcctcc gagcccgtcc tgaacgccaa cgacgagtgg gcgatcgagg aggcgatgcg
     2041 catcaaggag cgcacgcccg gcaccgaggt cgtcgcgctg tgcatgggcc cggacgccgc
     2101 ccagtcgatc gtgcgcaagg cgctgagcta cggcctcgac gccgccatcc aggtcaccga
     2161 ccccgcgctg cacggctccg acgccgtcgc caccgcgcgc gtgctggcca aggcgctcga
     2221 gggcgagacc tacgacctca tcatcatggg caaccagtcc tccgacgccc ggtcgatgct
     2281 cgtgcccgcg atgctcgccg agttcctcgg cctgcccgcg ctgacgttct gccagaagct
     2341 ggacgtcgac ggcaccacgt tccgcgccga gcgccagacc tccaatggct accaggtcgt
     2401 cgaggcgcag ggccccgtgg tcgtctccgt ggtggaggcg atcaacgagc cgcgctaccc
     2461 gtcgttcaag gggatcatgg ccgccaagtc caagccgctg accaccaagt cgctcggcga
     2521 cctgggtgtc gacggcggcg aggtcggcct ggacggctcc cggacgcagg tcatcgacgt
     2581 gacgccgcgc ccgcccaagc aggccggtga gaaggtcgag gacgacggct ccggcaccgt
     2641 gggcgccaag gcgctggccg acttcctggc tgccaagaag ttcgtgtagg cgacgacgag
     2701 cgaaaaggag aacacaccaa tggctgacat cctcgtcttc gtcgaccacg acaagggcac
     2761 ggtcaagaag gtcagcgcgc agatgctgac cgccgccaag aacaagggcg gcagcgtgag
     2821 cgccgcgatc ttcggtgagg gcgcgagcgg cgccgtcgag cgcgccggcg cgttcggcgc
     2881 gagcaaggcg tacgtgtggg aggccccgga ggc
//
LOCUS       DATMKC010000106         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_39547, whole genome shotgun sequence.
ACCESSION   DATMKC010000106 DATMKC010000000
VERSION     DATMKC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39547"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VNU01_03125"
     CDS             <1..734
                     /locus_tag="VNU01_03125"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWH31643.1"
                     /translation="TEYAVYAERLQPYIADTGTLLHEALESGETILLEGAQGTLLDLD
                     HGTYPFVTSSNPVAGGALTGAGLGPRHVDRVIGITKAYVTRVGAGPFPTELHDDIGER
                     MTAVGQEYGTTTGRRRRVGWFDAVLARYAARVNGLTEHFLTKLDVLSEFDTLRVCRAY
                     RYQGEEYDHFPPHQSIFHHAEPVYEELPGWRDDITQVAEYDDLPVQAKGYVERLEELC
                     GVPIRWVSVGPARAQTLDREPKLSL"
     gene            735..2003
                     /gene="purD"
                     /locus_tag="VNU01_03130"
     CDS             735..2003
                     /gene="purD"
                     /locus_tag="VNU01_03130"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWH31644.1"
                     /translation="MRVLVLGGGGREHALCWALARSGHEVLCGPGNAGIAEVARVVAL
                     DAEDPEAVVAAARDEGADLVVVGPEAPLVAGVADALAAAGVPVFGPTAKGAAIEGSKA
                     FAKAVMAEAGVPTAGYWTGTDAAEAKKALDGFAPPYVVKADGLAAGKGVRICADRAEA
                     EEAIDDAIVRRVFGEAGGTVVIEEFMTGPEVSLFGLCDGRTVVPMVPAQDFKRALDGD
                     QGLNTGGMGAYSPVPAFTPAMVADVRRDVLQPVLDALAARGIDYVGVLYAGLMLTPQG
                     VKVLEFNARFGDPETQVVLPRLRSDLGELLLACATGRLAAFGPLEWDERACVTVIMAS
                     GGYPGDYRTGVPIGGLAEAAALEDVMVFHAGTRADAGQTVTAGGRVLAVSAFGDGVAA
                     ARERAYEGVDRIGFAGAHVRRDIAARPTGG"
     gene            2034..3062
                     /locus_tag="VNU01_03135"
     CDS             2034..3062
                     /locus_tag="VNU01_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31645.1"
                     /translation="MSVLLVVLGFVLIVVALWDATSTMVSVEGGGPITSRIGQAVFRT
                     LARLSPDPHSRVQSKGGEAISFLVILLWITLLWAGWTVIFLSASGAVVDTATGDPAGL
                     VDTIYFAGFGLFTLGVGDFRPAPGFFQIATALSSLSGLFLVTVGISYLLSLTNAVAER
                     RGVAAVISGLGHSGEEILRTSWNGRDFSGLEHQLATLASGVAALAEQHLAKPVLHYFH
                     SPNRHTALEPSVVSLYEAVLLLQYAVEPGVRPRPPSLGATKAACVRLFEVYRQVFVRH
                     PVDEVAEPCLDALEEAGIPLRPAADVRRDLEGERETRLLAASLLAESGWPPASADYDA
                     PGYEPMRH"
     gene            3116..>3707
                     /locus_tag="VNU01_03140"
     CDS             3116..>3707
                     /locus_tag="VNU01_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWH31646.1"
                     /translation="MIPRYTLPEMGALWTDEARLRTWVEVEVLACEAHAELGVIPAGD
                     LAAIKQGTPPSPDEVTAIERETNHDVIAFLTAFMRTIPGEAGRWVHYGMTSSDLVDTA
                     YGATLARATDLVLAKADALVAVLKRRALEEWDTVCVGRTHGVQAEPTTFGHKLAQFAF
                     AVDRGRARLRAARTAIAVGSISGAVGTYSNIDPFVEA"
BASE COUNT          464 a         1309 c         1418 g          516 t
ORIGIN      
        1 agacggagta cgccgtctac gccgagcgcc tgcagcccta catcgccgac accggcacgc
       61 tcctgcacga ggcgctggag tcgggcgaga cgatcctgct ggagggcgcg cagggcacgc
      121 tgctggacct ggaccacggc acgtatccgt tcgtgacgtc gtccaacccg gtggccggcg
      181 gcgcgctgac cggcgcaggc ctggggccgc gccacgtcga ccgcgtcatc ggcatcacca
      241 aggcctacgt cacgcgggtc ggcgccggcc cgttccccac cgagctccac gacgacatcg
      301 gcgagcgcat gaccgccgtg ggccaggagt acggcaccac cacgggacgc cgccggcgcg
      361 tgggctggtt cgacgcggtg ctcgcgcgtt acgccgcccg cgtcaacggt ctgaccgagc
      421 acttcctcac caagctggac gtgctgagcg agttcgacac gctgcgggtg tgccgggcct
      481 accgctacca gggcgaggag tacgaccact tcccgccgca ccagtcgatc ttccaccacg
      541 ccgagccggt gtacgaggag ctaccgggct ggcgcgatga catcacgcag gtggccgagt
      601 acgacgacct gcccgtccag gcgaagggct acgtcgagcg gctggaagag ctgtgcggcg
      661 tgccgatccg ctgggtgtcc gtcggcccgg cgcgggcgca gacgctggac cgcgaaccca
      721 agctgtcgct ctagatgcgg gtcctggtcc tgggcggggg cggccgcgag cacgcgctgt
      781 gctgggcgct ggcgcggtcg ggccacgagg tgctgtgcgg cccgggcaac gcggggatcg
      841 cggaggtcgc gcgcgtggtc gcgctggacg ccgaggaccc ggaggccgtc gtggcggcgg
      901 cgcgggacga gggcgccgac ctcgtcgtgg tgggtcccga ggcaccgctg gtcgcgggcg
      961 tcgcggacgc gctggcggcg gccggggtgc cggtgttcgg ccccaccgcg aagggcgcgg
     1021 ccatcgaggg cagcaaggcg ttcgccaagg cggtcatggc cgaggcgggg gtgcccaccg
     1081 cgggctactg gacgggcacc gacgccgctg aggccaagaa ggcgctggac ggcttcgcgc
     1141 cgccgtacgt ggtgaaggcc gacgggctgg ccgcgggcaa gggggtgcgc atctgcgccg
     1201 accgggcgga ggcggaggag gcgatcgacg acgcgatcgt gcgccgggtg ttcggcgagg
     1261 cgggcggcac cgtcgtcatc gaggagttca tgaccgggcc cgaggtctcg ctgttcgggc
     1321 tgtgcgacgg gcgcaccgtg gtgccgatgg tgcccgcgca ggacttcaag cgcgcgctgg
     1381 acggcgacca ggggttgaac acgggcggca tgggcgccta ctcgccggtg ccggccttca
     1441 cgccggccat ggtggcagac gtgcggcgcg acgtgctcca gccggtgctc gacgcgctcg
     1501 cggcgcgcgg catcgactac gtcggcgtgc tgtacgcggg actgatgctc acgccgcagg
     1561 gcgtgaaggt gctggagttc aacgcgcgct tcggcgaccc ggagacgcag gtggtgctgc
     1621 cgcggctgcg cagcgacctg ggggagctgc tgctggcctg cgccaccggg cgcctggccg
     1681 cgttcggccc gctggagtgg gacgagcgcg cctgcgtgac ggtgatcatg gccagcgggg
     1741 gctacccggg cgactaccgc acgggggtgc ccatcggcgg cctggcggag gcggcggcgc
     1801 tggaggacgt catggtcttc cacgcgggga cgcgcgcgga cgccggccag accgtcacgg
     1861 cgggcggccg cgtgctcgcg gtgtccgcgt tcggcgacgg cgtcgcggcg gcgcgcgaac
     1921 gcgcgtacga gggcgtggac cgcatcggct tcgcgggcgc ccatgtgcgc cgcgacatcg
     1981 ccgcccggcc cacgggaggg tgaccgcttc tccggcgggg taggaccacg gcaatgtcgg
     2041 tcctgctggt cgtcctgggg ttcgtgctca tcgtggtggc cctgtgggac gccacgtcca
     2101 ccatggtgag cgtcgagggc ggcggaccca tcacctcgcg catcggccag gcggtgttcc
     2161 gcacgttggc gcggctctcg ccggacccgc actcgcgcgt gcagtccaag ggcggggagg
     2221 cgatctcctt cctggtgatc ctgctgtgga tcacgctgct gtgggcgggc tggacggtga
     2281 tcttcctgtc cgcttccggc gcggtcgtcg acacggccac cggcgacccg gccggcctgg
     2341 tcgacaccat ctacttcgcc ggcttcggtt tgttcaccct cggggtcggc gacttccggc
     2401 ccgcgcccgg cttcttccag atcgccacgg ccctgtcgtc gctgagcggg ctgttcctgg
     2461 tgaccgtcgg catctcctac ctgctgtcgc tgaccaacgc ggtggcggag cgccgcgggg
     2521 tggcggcggt catctccggc ctcggccaca gcggcgagga gatcctgcgc acgtcgtgga
     2581 acggccggga cttctccggg ctggagcatc agctcgccac actggccagc ggcgtggcgg
     2641 cgctggccga gcagcacctc gccaagccgg tgctgcacta cttccacagc cccaaccggc
     2701 acactgccct ggagccgagc gtcgtctcgc tctacgaggc cgtcctgctg ctgcagtacg
     2761 ccgtggaacc cggcgtgcgg ccacgtccgc cgtcactcgg cgccaccaag gccgcctgcg
     2821 tgcgcctgtt cgaggtgtac cggcaggtct tcgtccgcca ccccgtggac gaggtggcgg
     2881 agccgtgcct ggacgccctg gaggaggccg gcatccccct gcgcccggcc gccgacgtgc
     2941 gccgcgatct ggagggcgag cgcgagaccc ggctgctcgc ggcctcgctg ctggccgaga
     3001 gcggatggcc gccggcttcc gcggactacg acgcccccgg ctacgagccg atgcggcact
     3061 gaggccgggt ggcggcggtt cgccccacgg ccgcgcatgc gtcatgctct agcgcgtgat
     3121 cccgcgctac acgctccccg agatgggcgc cctctggacg gacgaggcgc gcctgcgcac
     3181 gtgggtcgag gtcgaggtgc tggcgtgcga ggcgcacgcc gagctcggcg tcatccccgc
     3241 cggcgacctg gccgccatca agcagggcac gccgccgtcc ccggacgagg tcacggcgat
     3301 cgagcgcgag accaaccacg acgtcatcgc gttcctcacc gcgttcatgc gcaccattcc
     3361 cggtgaagcc gggcgctggg tgcactacgg catgacgtcc tccgacctgg tcgacaccgc
     3421 ctacggcgcc accctggcgc gcgcgaccga cctggtgctg gccaaggccg acgccctggt
     3481 ggccgtgctg aagcggcggg ccctggagga gtgggacacc gtctgcgtcg ggcggacgca
     3541 cggcgtgcag gccgagccca cgaccttcgg ccacaagctg gcgcagttcg ccttcgcggt
     3601 ggaccgcggc cgcgcgcgcc tgcgcgccgc gcgcacggcg atcgcggtgg gctccatcag
     3661 cggcgcggtg gggacctact ccaacatcga cccgttcgtg gaggcct
//
LOCUS       DATMKC010000107         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_302033, whole genome shotgun sequence.
ACCESSION   DATMKC010000107 DATMKC010000000
VERSION     DATMKC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302033"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..372
                     /locus_tag="VNU01_03145"
     CDS             43..372
                     /locus_tag="VNU01_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31647.1"
                     /translation="MSQPSTPKYGATLTEIIATLEAAGFTGQMAARAGGAMQCLTCRQ
                     ESPANDFRLGAMRRTEGASDPADMLAVVGITCPKCGAQGTAVLGYGPEADPDDAEAVL
                     RLGVLES"
     gene            complement(369..755)
                     /locus_tag="VNU01_03150"
     CDS             complement(369..755)
                     /locus_tag="VNU01_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWH31648.1"
                     /translation="MADDGKELVTRYLEEAWNAGNVDVVDELFTPELAARLKPLIGAF
                     RAGFPDWHCVIDDFVVEGDKVVNRWTGKATHTGNFFGIPPTGKPVTVEGITIHQIADG
                     RIVNDWSQSDQLALMQQLGVAPGPQG"
     gene            863..1198
                     /locus_tag="VNU01_03155"
     CDS             863..1198
                     /locus_tag="VNU01_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWH31649.1"
                     /translation="MDTTQLLKGVLDLAVLAVVRDEDGYGYDIVRRLRTAGLDDVGDA
                     SVYGTLRRLYQAGALKSYVVASEEGPHRKYYGATPAGRDLYETWIKKWRGFAEAMDVL
                     IDTPERTTA"
     gene            1195..>2033
                     /locus_tag="VNU01_03160"
     CDS             1195..>2033
                     /locus_tag="VNU01_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31650.1"
                     /translation="MSSTLDFGATGDTATRDAERYLKDVAPHLAALPAEERADLLDDL
                     AQHLREIAAEEGPPLVERLGPPEAYAAELLASAGVALGRRPGVPLLDRAARVVERGRR
                     SWAGQELMRLSPALRPAWWVARAFLAVAFLAEAGGGSADVFPIPELAGSRLIGLLAIL
                     AAIPLSVRLAERSLPRSGRLLVAGAGAVLAVYGLSLLNGAGSADVHYVDAYGPPSFAD
                     RGCLTASSGRPITNLYAYDSEGRLLDPVLLFDQDGQPVDNLCPEADWRGRQLFTEYRQ
                     DVNG"
BASE COUNT          283 a          742 c          709 g          299 t
ORIGIN      
        1 cctgctggag gacaacgtga cactacgggt agaagtggcc agttgagcca gccgtcgacg
       61 cccaagtacg gcgccaccct gaccgagatc atcgccaccc tggaggccgc cgggttcacc
      121 ggccagatgg cggcccgcgc cggcggtgcc atgcagtgcc tgacctgccg ccaggagagc
      181 ccggccaacg acttcaggct gggtgcgatg cgccgcaccg agggcgcctc cgacccggcc
      241 gacatgctgg cggtggtggg catcacctgt cccaagtgcg gggcccaggg gaccgcggtg
      301 ctgggctacg gcccggaagc ggatcccgac gacgccgagg ccgtcctccg tctcggggtg
      361 ctcgagagct aaccctgggg gccgggggcg accccgagct gctgcatgag cgccagctgg
      421 tcgctctggc tccagtcgtt gacgatgcga ccgtcggcga tctggtggat ggtgatgccc
      481 tccacggtga ccggcttgcc ggtcggaggg atcccgaaga agttgccggt gtgggtggcc
      541 ttgcccgtcc accggttgac caccttgtcg ccttcgacga cgaagtcgtc gatcacgcag
      601 tgccagtccg ggaagccggc gcgaaaggcg ccgatgagcg gcttcagccg ggcggccagc
      661 tcgggcgtga acagctcgtc gaccacgtcg acgttgccgg cgttccacgc ctcctcgaga
      721 taccgggtga ccagttcctt gccgtcgtca gccatcggtc gcctccctta cacgccagcc
      781 gccatcgacc ctagcgtctg tcaccacgct ttcccactac tgcggattcc gtggtaccgt
      841 gcagaacgga gaagggaggc gtatggacac cacgcagctc ctcaaagggg tgctcgacct
      901 ggccgtactg gcggtggtac gcgacgagga cgggtacggg tacgacatcg tccgccgcct
      961 gcgcaccgcc ggcctcgacg atgtcggtga cgcctcggtg tacggcaccc tccgccggct
     1021 gtaccaggcg ggggcgctga agtcctacgt ggtggcgtcg gaggaaggcc cccaccgcaa
     1081 gtactacggc gccacgccgg ccgggcgcga cctgtacgag acgtggatca agaagtggcg
     1141 gggcttcgcg gaggccatgg acgtgctgat cgacaccccg gaaaggacga ccgcgtgagc
     1201 tccaccctgg acttcggcgc caccggcgac acggcgactc gggacgccga gcggtacctg
     1261 aaggacgtcg ccccccacct ggccgccctg ccggccgagg agcgcgccga cctcctcgac
     1321 gacctggccc agcatcttcg ggagatcgcg gccgaggaag gtccgccgct ggtcgagcgg
     1381 ttggggccgc ccgaggccta cgccgccgag ctgctggcct ccgccggcgt ggcactcggc
     1441 cggcggccgg gcgtgccgtt gctcgaccgc gccgcccgcg tggtcgagcg cggtcgccgg
     1501 tcgtgggccg gccaggagct gatgcgcctg tcgcccgcgc tccggccggc gtggtgggtg
     1561 gcccgggcct tcctcgccgt cgccttcctg gccgaggccg gcggcgggag cgccgacgtc
     1621 ttcccgatcc cggagctggc cggcagccgg ctgatcggcc tgctggccat cctcgcagcc
     1681 atcccactgt ccgtgcgcct ggccgagcga tcccttcccc gcagcggccg gctcctggtc
     1741 gccggcgccg gcgccgtgct ggccgtctac gggctctcgc tcctcaacgg cgccggttcg
     1801 gccgacgtcc actacgtcga cgcgtacggc ccaccgagct ttgccgaccg aggctgtctc
     1861 accgcgtcgt cgggccggcc catcaccaac ctctacgcct acgacagcga gggccggctg
     1921 ctcgaccccg ttctcctctt cgaccaggac ggccagcccg tcgacaacct gtgcccagaa
     1981 gccgactggc ggggccggca gctcttcacc gagtaccgcc aggacgtgaa tgg
//
LOCUS       DATMKC010000108         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_337489, whole genome shotgun sequence.
ACCESSION   DATMKC010000108 DATMKC010000000
VERSION     DATMKC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337489"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VNU01_03165"
     CDS             complement(<1..736)
                     /locus_tag="VNU01_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HWH31651.1"
                     /translation="MEATHAMAALGSRASSRTASPGARTTRTTVPPTASGSERAGGPA
                     TRGTAGTSATVSTGAGAATGADGVGSCEFPSVPPGDYLVVQTAAPEGHAAAAEPHPVA
                     LAAGEARTITFTNASDMSTVEVSATDGAGAPLEGAAFAAYADADSDGTVAPGAAPAAT
                     CVTGPGGTCRMRLPAGAYVLVQTAAPAGLEPMDPLAFRFATGGQVAAVTVRNATPAAG
                     APAPAAAPVYSAPVAAPARSRPARSRP"
     gene            951..1565
                     /locus_tag="VNU01_03170"
     CDS             951..1565
                     /locus_tag="VNU01_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31652.1"
                     /translation="MRIRATGLLAAIGLLVGMMAAPASADAPTDAGIFNGQASVSQDR
                     GTDQTKWCQPDGTFAGLNTQTGVGLPLLGGVDKAYWKIDAPQGAVISLARVATGSLQM
                     CGELAPLTTSPAQLGASCITTKGSNGQGFGDFDGDQIWLSGLSWEATVGGTFTVTAKA
                     GSTQGDTGSDLVAIVQALDEAVLLNCLSQNGARTFNVQAVYAVV"
     gene            complement(1629..1943)
                     /locus_tag="VNU01_03175"
     CDS             complement(1629..1943)
                     /locus_tag="VNU01_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31653.1"
                     /translation="MSAEYPREPREPVRDREVIVSEPAGGGPGGVIAVIIGIVALVLV
                     VWLLISMLGGDGEQNLEIDGGVDVPEEIDVNVDNGGGTDTTGGTDTTGGTDTGGDTAA
                     DG"
BASE COUNT          250 a          734 c          771 g          261 t
ORIGIN      
        1 ccggtctact ccgcgccggt ctactccgcg ccggcgcggc gaccggcgcg gagtagaccg
       61 gcgcggccgc cggtgcgggc gcgccagcgg ccggcgtggc gttgcggacc gtgacggcgg
      121 cgacctggcc gcccgtcgcg aagcggaacg ccaacgggtc catgggctcc aggccggccg
      181 gcgcggcggt ctgcaccagc acgtaggcgc ccgccggcag gcgcatgcgg cacgtgccgc
      241 cgggtccggt cacgcaggtc gccgcaggcg cggcgccggg ggcgaccgtg ccgtcgctgt
      301 cggcgtcggc gtaggccgcg aaggccgcgc cctccagtgg cgcgccggcg ccgtcggtgg
      361 cgctgacctc gaccgtcgac atgtcggagg cgttcgtgaa cgtgatcgtc cgcgcctcgc
      421 cggcggccag cgcgacgggg tgcggctcgg cggctgcggc atggccctcc ggcgcggccg
      481 tctgcaccac caggtagtcc ccgggcggca cggacgggaa ctcgcaggag ccgacaccgt
      541 ccgcgccggt cgccgcgccg gcgccggtgg agaccgtcgc ggaggtcccg gcggtgccgc
      601 gggtcgccgg cccgccggcc cgctcgctgc ccgaggcggt gggcgggacc gtggtgcgcg
      661 tcgtccgcgc gcccggcgac gcggtgcggc tgctggcccg ggagcccagg gccgccatcg
      721 cgtgggtggc ctccatcgcc ctgctgtgcc tggccgcact cgccgtgcgc cggcggcatc
      781 gaggactcgt tctgatcgac cagagaggag cgagcacgcc gtagcggcgg atccgtcacg
      841 gcagacacgc gtcgcggggc acggacggcg gggtccgtgc ccggtgggac gacgcacgca
      901 tcgccgggcg cctcgcaaca ggcgcgccgc agatctttcg aggaggagca atgcgcatca
      961 gggctacagg actgctggcc gccatcggcc tgctcgtcgg catgatggcc gcgccggcat
     1021 ccgccgacgc gccgaccgac gccggcatct tcaacggaca ggcgtcggtc agccaggacc
     1081 gtgggacgga ccagaccaag tggtgccagc cggacggcac cttcgccggc ctcaacaccc
     1141 agaccggcgt gggtctgccg ctgctgggcg gcgtggacaa ggcctactgg aagatcgacg
     1201 ccccgcaggg cgccgtgatc tccctggcac gggtggcgac gggctcgctg cagatgtgcg
     1261 gggaactcgc ccccctgacc acgagcccgg cccagctcgg tgcctcctgc atcaccacca
     1321 agggcagcaa cgggcagggc ttcggcgact tcgacggcga ccagatctgg ctgagcgggt
     1381 tgagctggga ggccaccgtg ggcggcacgt tcacggtgac ggccaaggcc ggcagcacgc
     1441 agggcgacac gggcagcgac ctggtggcga tcgtgcaggc gctggacgag gcggtgctgc
     1501 tgaactgcct cagccagaac ggcgcccgga ccttcaacgt gcaggcggtg tacgcggtcg
     1561 tgtgacgctg gatcacgcag gcggcccccg gcgtgatcgc cgggggccgc tgtcgctccg
     1621 gggcggtgct atccgtccgc ggccgtgtcg cccccggtgt cggtgccgcc ggtggtgtcg
     1681 gtgccgccgg tggtgtcggt gccgccaccg ttgtcgacgt tgacgtcgat ctcctccggg
     1741 acgtccacgc cgccgtcgat ctccaggttc tgctccccgt cgccgccgag catgctgatc
     1801 agcagccaca ccaccagcac gagggccacg atcccgatga tgacggcgat gacgcctccc
     1861 gggcctccgc ccgcgggctc gctgacgatg acttcacggt cgcgcaccgg ctctcgcggc
     1921 tcgcgcgggt actcggcgct catggggacc tcctgggtcg gtcgaaaggg gctttgcctg
     1981 gctaccctgc gtgcatggac gacaaacggt ccgcac
//
LOCUS       DATMKC010000109         2427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_200036, whole genome shotgun sequence.
ACCESSION   DATMKC010000109 DATMKC010000000
VERSION     DATMKC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200036"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VNU01_03180"
     CDS             <1..313
                     /locus_tag="VNU01_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31654.1"
                     /translation="TRGDEGPGPVRVAETRLTPPPPARRQAGEVLQAPTEPVPLVDTH
                     ARTFETHAEELADKPAADVIRAARDLSTDELRALYEYEQGHKKRKTVLQEIEAQLAPV
                     G"
     gene            complement(390..>2427)
                     /gene="purL"
                     /locus_tag="VNU01_03185"
     CDS             complement(390..>2427)
                     /gene="purL"
                     /locus_tag="VNU01_03185"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWH31655.1"
                     /translation="ILVGPGENAGVVDVGNGQAVTFKIESHNHPSFVEPFQGAATGVG
                     GIIRDILTMGARPIALMDPLRFGDPASDKTRWVVDGVVQGIGHYGNSIGVATVGGETV
                     FDPCYNGNPLVNVFCVGVMDADRIQLAVAERVGDIAVLIGQRTGRDGIGGASVLASAE
                     FGEDGVDEAKRPNVQVGDPFAGKLLIESCLQLYAEDLLSGIQDMGAAGIACSTSEMAA
                     KAGMGMRVDLDKVPLREPSMEAWEILCSESQERMLALVSPDRLDRVLEICRRWGVDAT
                     PIGEVVEGDRLVFTRHGEVVHDAPAKALVDEGPVYHRPVEDWVHPEAHEDVDAWPAPA
                     DLGEAVRQVLSSPNVGDARWVFQQYDSIVQHGTVQGPGSDAAVLRLPATGAEDTEYPR
                     GIAISTDGNGRWCALDPEEGAKLVVAEAARNVACTGAVPVAATNCLNFGSPERPEIMG
                     QFRDTIAGLSAACEALGTPITGGNVSFYNQTGDTAIHPTPVIGVLGVFDQVVTQVPNA
                     FREAGDVLFLVGAPTRPGLGGSEYLWRVHGRIAGRPPRIDLEEERRLHGLLWAAAEAQ
                     WLRSAHDVSTGGLVVTLAESALPAMLGARVIPEPGVEPVQFMFSESPSRVVVSLKAPD
                     AEQFRRLCEARGVHCQKLGMVIEEPVLAFQDLLDIDLAEARSLHDTALEDALHS"
BASE COUNT          343 a          897 c          856 g          331 t
ORIGIN      
        1 cacccgcggg gacgaggggc ccgggccggt gcgcgtggcg gagacccggc tcacgccgcc
       61 gcctccggcg cggcgccagg ccggcgaggt gctccaggcg ccgacggagc cggtcccgct
      121 ggtggacacg catgcccgca cgttcgagac ccacgccgag gagctggccg acaaacccgc
      181 cgccgacgtg atccgcgcgg cgcgcgacct gtccacggac gagttgcgcg ccctgtacga
      241 gtacgagcag gggcacaaga agcgcaagac cgtgctgcag gagatcgagg cgcagctcgc
      301 ccccgtcggc tgagccgatc cacagactcc ccctggaagc gggtgcagtt tggcgcacat
      361 acgcgccgaa ctgcacccgc ttcaggtggt cagctgtgga gggcgtcctc gagggcggtg
      421 tcgtggaggc tgcgggcctc ggcgaggtcg atgtcgagca ggtcctggaa cgcaaggacc
      481 ggctcctcga tcaccatgcc gagcttctgg cagtgcacgc cgcgcgcctc gcacaagcgg
      541 cggaactgct ctgcgtccgg cgccttgagg ctcaccacca cgcgtgacgg cgactcgctg
      601 aacatgaatt gcaccggctc gaccccgggc tcggggatga cgcgcgcgcc gagcatcgcg
      661 ggcagcgccg actccgccag cgtgaccacc aggccgcccg tggacacgtc gtgggcgctg
      721 cgcagccact gcgcctcggc ggcggcccac agcaggccgt ggaggcggcg ctcctcctcc
      781 aggtcgatgc gcggcgggcg cccggcgatg cgcccgtgca cgcgccacag gtactccgac
      841 ccgcccaggc cgggccgcgt gggcgcgccc accaggaaca gcacgtcgcc cgcctcccgg
      901 aaggcgttgg gcacctgggt gacgacctgg tcgaacacgc cgagcacgcc gatcaccggt
      961 gtggggtgga tcgccgtgtc gccggtctgg ttgtagaagc tcacgttgcc gccggtgatg
     1021 ggcgtgccca gcgcctcgca ggcggcgctc aggccggcga tggtgtcgcg gaactggccc
     1081 atgatctccg gccgctcggg gctgccgaag ttcaggcagt tggtcgcggc caccggcacg
     1141 gcaccggtgc aggcgacgtt gcgcgccgcc tccgcgacga cgagcttggc gccctcctcg
     1201 gggtccagcg cgcaccagcg gccgttgccg tccgtggaga tcgcgatgcc ccgcgggtac
     1261 tcggtgtcct ccgcgcccgt cgcgggcagg cgcagcaccg ccgcgtccga gccaggaccc
     1321 tgcacggtgc cgtgctgcac gatcgagtcg tattgctgga acacccagcg ggcgtcaccg
     1381 acgttgggtg agctcagcac ctgccgcacg gcctcgccga ggtccgccgg ggcgggccag
     1441 gcgtcgacat cctcgtgcgc ctcggggtgc acccagtcct cgacgggacg gtggtagacc
     1501 ggcccctcgt ccaccaatgc cttggcgggc gcgtcgtgca cgacctcgcc atggcgggtg
     1561 aacaccagcc ggtcgccctc caccacctcg ccgatcggcg tggcgtccac gccccagcgc
     1621 cggcagatct ccagcacgcg gtccagcctg tcgggcgaca ccagcgccag catgcgctcc
     1681 tggctctccg agcacaggat ctcccacgcc tccatggacg gctcgcgcag cggcaccttg
     1741 tccaggtcca cgcgcatgcc catgccggcc ttggcggcca tctcggaggt ggagcaggcg
     1801 atgcccgccg cgcccatgtc ctggatgccg ctcagcaggt cctcggcgta cagctgcagg
     1861 cagctctcga tcaagagctt gcccgcgaac gggtcgccca cctgcacgtt tgggcgcttc
     1921 gcctcgtcca cgccgtcctc gccgaactcg gcggaggcca gcacggaggc accgccgatg
     1981 ccgtcgcggc cggtccgctg cccgatcagc acggcgatgt cgcccacgcg ctcggccacc
     2041 gcgagctgga tgcggtcggc gtccatgacg cccacgcaga acacgttgac cagcgggttg
     2101 ccgttgtagc aggggtcgaa cacggtctcg ccgcccacgg tggccacgcc gatggagttg
     2161 ccgtagtggc cgatgccctg caccacgccg tccacgaccc agcgcgtctt gtcggaagca
     2221 ggatcgccga agcgcagcgg gtccatgagg gcgatgggcc gggcgcccat ggtgaggatg
     2281 tcgcggatga tcccgccgac gccggtcgcc gcgccctgga acggctccac gaagctggga
     2341 tggttgtggc tttcgatctt gaaggtgacc gcctggccgt tgccgacgtc caccacaccg
     2401 gcgttctcgc cgggtccgac caggatg
//
LOCUS       DATMKC010000110         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_124600, whole genome shotgun sequence.
ACCESSION   DATMKC010000110 DATMKC010000000
VERSION     DATMKC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124600"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /gene="serA"
                     /locus_tag="VNU01_03190"
     CDS             complement(<1..1499)
                     /gene="serA"
                     /locus_tag="VNU01_03190"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWH31656.1"
                     /translation="MKVLVADKLSEAGVQALAEHFDVDVNTGLPKDELLKVVPDYDAI
                     VIRSATTVDADVIEAAGKLKVIARAGIGLDNVDVKAATARGVIVCNAPQSNIISAAEH
                     TVALLLSLARRIPEADTSLKGGEWKRSALEGTELHGKVLGVLGLGRIGVLVAQRLSAF
                     GMKLVAYDPFISAERAARMGVELVPTVDELIAVADVVTIHLPKTPETVGIIGEAQLKA
                     MKPGALLVNCARGGLVDEDALYVALSERWIAGAALDVFSAEPMTDSKLFTLDNIVVTP
                     HLGASTTEAQDKAGTQVAEAVVLALRGDFVPSAVNVAITAGIPEELKPFMPLTEKLGR
                     LFTALNGGNPSEITLECVGKIADANTEALTLSSIRGLLTDVVEEPVTFVNAPQLASDR
                     GLNVSTLTSHESRDYVSVVRLSGGGLRVAGTLVGPSDRERLVEVWGFDLDMEPTDHML
                     FFRYADVPGVVGTIGGKLGEAGVNIAGMQVGRREAGGEALMALSVDSAM"
     gene            1685..1933
                     /locus_tag="VNU01_03195"
     CDS             1685..1933
                     /locus_tag="VNU01_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWH31657.1"
                     /translation="MDVSATLTSKGQVTIPREVREALGLHEGDRVIFRVVGDTAVLAR
                     TDDLLDLAGTIPVPARASALTPGEERRAAHRAQTRRSA"
     gene            1933..2316
                     /locus_tag="VNU01_03200"
     CDS             1933..2316
                     /locus_tag="VNU01_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWH31658.1"
                     /translation="MQVDTNVLVRHVTGSPAAQARRATAFLRQAERLDLPDLIVAELV
                     SVLESVYRQPRPAVAAVVRAVVAFPAIRVGDERLVLRAIELYETQRVDFAEAYLAAAA
                     ERTDGQVVSFDRGLDRIGSVRRIEP"
BASE COUNT          353 a          889 c          886 g          355 t
ORIGIN      
        1 ctcatggcgc tgtcgacgga cagcgccatg agcgcctcgc ccccggcctc gcgccggccg
       61 acctgcatgc cggcgatgtt cacaccggcc tcgcccagct tgccgccgat cgtgcccacg
      121 acccccggca cgtccgcgta gcggaagaac agcatgtggt cggtgggctc catgtccagg
      181 tcgaagcccc acacctccac caggcgctcg cggtcgctgg ggccgaccag ggtgccggcc
      241 acgcgcagcc cgccaccgga caggcgcacc acggagacgt agtcgcgcga ctcgtgcgac
      301 gtcagggtgg agacgttcag gccgcgatcg gaggcgagct gcggcgcgtt gacgaacgtg
      361 accggctcct ccaccacgtc ggtgagcagg ccgcggatgg acgacagcgt cagcgcctcc
      421 gtgttcgcgt cggcgatctt gccgacgcac tccagcgtga tctcgctggg gttgccgccg
      481 ttgagcgcgg tgaacagccg gcccagcttc tccgtgagcg gcatgaaggg cttgagctcc
      541 tcggggatgc cggcggtgat ggccacgttg accgcgctgg gcacgaagtc gccgcgcagc
      601 gccagcacca ccgcctcggc gacctgcgtg ccggccttgt cctgcgcctc ggtggtcgag
      661 gcgcccaggt gcggcgtgac cacgatgttg tccagcgtga acagcttgga gtccgtcatc
      721 ggctcggcgg agaacacgtc cagcgcggcg ccggcgatcc agcgctccga cagggcaacg
      781 tacagcgcgt cctcgtcgac caggccgccg cgggcgcagt tgaccaggag ggcgcccggc
      841 ttcatcgcct tgagctgcgc ctcgccgatg atgcccacgg tctcgggggt cttgggcagg
      901 tggatcgtga cgacgtcggc cacggcgatg agctcgtcca ccgtcggcac cagctccacg
      961 cccatgcgcg cggcgcgctc ggcggagatg aacgggtcgt aggcgaccag cttcatgccg
     1021 aacgccgaca ggcgctgcgc gaccagcacg ccgatgcgcc ccaggcccag cacgccgagg
     1081 accttgccgt gcagctcggt gccctccagc gcgctgcgct tccactcccc gcccttgagc
     1141 gaggtgtccg cctccgggat gcggcgggcc agcgacagca gcagcgcgac ggtgtgctcg
     1201 gcggcggaga tgatgttgga ctgcggcgcg ttgcagacga tcacgccgcg cgcggtcgcg
     1261 gccttcacgt cgacgttgtc caggccgatg cccgcgcgcg cgatcacctt gagcttgccc
     1321 gccgcctcga tgacgtcggc gtccacggtg gtcgcggagc ggatcacgat ggcgtcgtag
     1381 tcgggcacga ccttgagcag ctcgtccttg ggcagcccag tgttgacgtc cacgtcgaag
     1441 tgctccgcca gggcctgcac accggcctcg gagagcttgt cggcaacgag caccttcatg
     1501 tggggcgatt ccttcgtcgg tgacggatgc gccgtgcgcc gcgcgggcac ggagcacaac
     1561 tgtagggctc accgaacagc gcggccaaag cgcggcgcgc gtccgggcct gtggacgagc
     1621 cgatggcccg ccgggcggca cgcgcgtata atctccttac ccactgataa tgtaaggagg
     1681 cggcatggac gtcagcgcca cgttgacctc gaagggacag gtgacgatcc cgcgagaggt
     1741 ccgtgaggca ctgggtctcc acgagggaga ccgggtgatc ttccgcgtgg tgggcgacac
     1801 cgccgtgctc gcgcggaccg acgacctgct cgatctcgcc gggaccatcc ccgtgcccgc
     1861 gcgcgcgtcg gcgctcaccc cgggcgagga gcgccgggcg gcccaccggg cacagacgcg
     1921 ccggagcgcg tagtgcaggt cgacaccaac gtcctggtcc gccatgtgac cggctcgccg
     1981 gcggcgcagg cgagacgcgc caccgcgttc ctgcgccagg ccgagcggct cgacctcccc
     2041 gacctgatcg tggccgagct ggtctccgtc ctggaatccg tctaccggca gccgcggccg
     2101 gcggtggcgg cagtggtgag agccgtggtg gcgttcccgg cgatccgggt cggcgacgag
     2161 cggctggtgc tgcgcgcgat cgagctgtac gagacccagc gcgtcgactt cgcggaggcg
     2221 tacctggccg ccgccgccga gcggaccgac ggccaggtcg tgtccttcga ccgcggcctg
     2281 gaccgcatcg gctccgtgcg gcgcatcgag ccctgaggcg cgccgccgtc tacaggcgca
     2341 ggccctggaa gacctcgcgc gtcgccgtgg agcggttcag cgtgaagaag tggatgccgg
     2401 gcgcgccgcc gtccaacagc tcctggcaca actcggtggc gaactccacg ccgatgcgcc
     2461 gcacggcttc cgggtcgtcc tcc
//
LOCUS       DATMKC010000111         2935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_97544, whole genome shotgun sequence.
ACCESSION   DATMKC010000111 DATMKC010000000
VERSION     DATMKC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97544"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..244)
                     /locus_tag="VNU01_03205"
     CDS             complement(14..244)
                     /locus_tag="VNU01_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31659.1"
                     /translation="MVRITPADTGSRVSVRRRIPHEQGQPSMTDVVGVLESWSDGVLR
                     IRTKRGELVDVDEPSLVAGRIISVGFPQTGGE"
     gene            complement(256..909)
                     /locus_tag="VNU01_03210"
     CDS             complement(256..909)
                     /locus_tag="VNU01_03210"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH31660.1"
                     /translation="MTSAAVTIAGPDGSVLRGRLRLADGAPRGAVVLCHPHPAFGGNM
                     DVWLLPAIGQALAADGWHVLRFDFRSVRTGVDPRGHLDGHAAERADVAAAVGHLREEG
                     LADGRLVVAGWSFGALVALLHGLDDERVTDWVGIAPAVAPLEELPMSAVPVARVRAWA
                     ARRVVVAGEHDQFFAPERMAAVSPHALRVIGGTDHFFFDRDAEVASAVAECLREPAP"
     gene            complement(947..1786)
                     /locus_tag="VNU01_03215"
     CDS             complement(947..1786)
                     /locus_tag="VNU01_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWH31661.1"
                     /translation="MPDDAPSLAALLRGAERTVVFTGAGVSTDSGIPDFRSPGGIWTR
                     YDPREFTFDRYVESADTRRRSWAMRREFWAVAPRPNAAHLAVAALESAGRVSGVMTQN
                     IDGLHQAAGSRVVVELHGTAREVTCIGASPRGGVPDGCGFRADTRWAFDRIDAGDEDP
                     ACPRCGGLVKSATVSFGQAMDAGALERAAMLFAHADLVLAVGSSLQVYPAAALPERAA
                     AAGVPLVIVNAEPTPLDDLAALVLHTRATPLLQEVAALVLEDGPAVDRRDGARAGPRP
                     SER"
     gene            complement(1820..2446)
                     /locus_tag="VNU01_03220"
     CDS             complement(1820..2446)
                     /locus_tag="VNU01_03220"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWH31662.1"
                     /translation="MTDLRTLRRAGGDALRLLVDLLPPDWDPRSGEEETSIVFADVSG
                     YSDFVAATGDDTALLVLAALDDAVAGALNGRKGARVVKRLGDGIMLAARRAGDGPAIA
                     AELVAAFERAAQSHEWPLRLRAGVHRGVVRRQGEDRIGYHVNLAARVAEAAPPGRTLA
                     SEAALAGVDLAREGLAARPAGRLHAKGVNGPVAVRTVWPDPAARRAAS"
     gene            complement(2539..>2935)
                     /locus_tag="VNU01_03225"
     CDS             complement(2539..>2935)
                     /locus_tag="VNU01_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWH31663.1"
                     /translation="DLLAVGLRPTRQRRSVVEALRERDDAVTAQELHASLRSAGEPIG
                     LATVYRTLTALAEAGFLDTFEREGEQAFRLCGEQHHHHLVCEACNRVEEIDAAEVEAW
                     VEAVARRRGYTVTAHRADVFGLCGRCTNG"
BASE COUNT          383 a         1158 c         1038 g          356 t
ORIGIN      
        1 cttcagtcta accctactcg cccccggttt gcggaaatcc gacagaaatg attcgcccag
       61 caaccagaga cggctcatcg acgtccacca gctccccgcg cttggtgcgg atccgcagga
      121 caccatcgga ccaggattcc agcacgccca ccacatccgt catgctcggc tgcccctgct
      181 cgtgcggaat ccgccgccga acgctcaccc ggcttcccgt atcggcgggc gtgatgcgga
      241 ccacgccgcg tgccctcatg gcgccggctc gcgcaggcac tcggcgaccg ctgacgccac
      301 ttcggcgtcg cggtcgaaga agaagtggtc ggtgccgccg atcacgcgca gcgcatgggg
      361 tgacaccgcc gccatgcgct ccggggcgaa gaactggtcg tgctcgccgg ccaccaccac
      421 gcggcgggcg gcccaggcgc gcacgcgcgc cacgggcacc gcgctcatcg gcagctcctc
      481 caggggggcc accgcgggcg cgatcccgac ccagtccgtc acgcgctcgt cgtccaagcc
      541 gtgcagcagc gcgaccagcg cgccgaagct ccagcccgcc accaccaggc ggccgtccgc
      601 cagtccctcc tcccgcaggt ggccgaccgc cgcggccacg tcggcgcgct cggcggcgtg
      661 accgtccaga tgcccgcgcg ggtccacacc cgtgcgcacg gagcggaagt cgaagcgcag
      721 cacgtgccag ccgtccgcgg ccagcgcctg gccgatcgcc ggcagcagcc acacgtccat
      781 gttgccgccg aacgcgggat gcgggtggca cagcacgacg gcgccgcggg gcgcaccgtc
      841 cgcgagccgc agccggcccc gcagcacgga accgtccggc cccgcgatgg tcactgctgc
      901 gctggtcacg atccggccct ctccctcgct cggcgggaaa ccctttttag cgctcgctcg
      961 gtcgagggcc ggctcgtgcc ccatcgcgcc ggtccactgc tggtccgtcc tcgagcacga
     1021 gggcggccac ctcttgcagc aagggggtgg cgcgggtgtg caggaccagg gcggcgaggt
     1081 cgtccagggg tgtgggctcg gcgttgacga tcaccaacgg gacgccggcg gcggcggccc
     1141 gctcgggcag cgcggccgcc gggtagacct gcagcgacga gccgacggcc agcaccaggt
     1201 ccgcgtgggc gaacagcatg gcggcacgct ccagggcccc cgcgtccatc gcctggccga
     1261 agctcaccgt ggcggacttc accaggccgc cgcagcgcgg gcacgccgga tcctcgtcgc
     1321 ccgcgtcgat gcggtcgaac gcccagcgcg tgtccgcgcg gaacccgcag ccgtccggca
     1381 cgccgccgcg cgggctcgcg ccgatgcagg tcacctcgcg cgcggtgccg tgcaactcga
     1441 ccacgacgcg cgagcccgcc gcctggtgca gcccgtcgat gttctgggtc atcacgccgg
     1501 acacgcggcc ggccgactcg agggcggcca cggccaggtg cgcggcattc gggcgcggcg
     1561 ccaccgccca gaattcccgg cgcatcgccc aggagcggcg cctggtgtcg gcggactcca
     1621 cgtagcggtc gaacgtgaac tcgcgggggt cgtagcgcgt ccagatcccg ccgggggagc
     1681 ggaagtccgg gatcccggag tcggtggaga cgccggcgcc ggtgaacacc accgtgcgct
     1741 cggcgccgcg cagcagcgcg gcgagggacg gcgcgtcgtc gggcatcgca ccacggtagc
     1801 ccgcgcggcg tgcgcgggct cagctcgcgg cgcgccgcgc cgccgggtcc ggccacaccg
     1861 tccgcacggc gaccggcccg ttcacgccct tggcgtgcag ccggccggcc ggtcgcgccg
     1921 ccagcccttc ccgggccagg tcgaccccgg ccagggcggc ctcgctcgcc agggtgcgcc
     1981 ccggcggcgc cgcctcggcc acgcgcgcgg ccaggttgac gtggtagccg atgcggtcct
     2041 cgccctggcg ccgcaccacg ccgcgatgga cgccggcgcg cagccgcagc ggccattcgt
     2101 gtgactgcgc ggcgcgctcg aacgccgcga cgagctcggc ggcgatggcc ggaccgtcgc
     2161 cggcgcgccg cgccgcgagc atgatgccgt cgcccagccg cttcaccacg cgcgcgccct
     2221 tgcggccatt gagcgccccg gcgaccgcgt cgtccagcgc agccagcacc agcagcgcgg
     2281 tgtcgtcgcc cgtggcggcg acgaagtcgc tgtagccgct cacgtcggcg aacacgatgc
     2341 tggtctcctc ctcgccgctg cggggatccc agtccggcgg gagcaggtcc acgagcaggc
     2401 ggagcgcgtc ccccccggcg cggcgcagcg tgcgcaggtc ggtcatggcg tccctctgcc
     2461 ggtgcttgca acagttatga agccgttagc ttcctgctag caggctacca gcatgtgggc
     2521 gggtttggcc gcagcgggtc agccgttggt acaacggccg cacaagccga agacgtccgc
     2581 gcggtgcgcg gtcacggtgt agccacggcg gcgcgccacg gcctccaccc aggcctccac
     2641 ctccgcggcg tcgatctcct ccacgcggtt gcaggcctcg cacaccaggt gatggtggtg
     2701 ttgctccccg cacagccgga acgcctgctc gccctcgcgc tcgaaggtgt ccaggaagcc
     2761 ggcctccgcc agcgcggtca gcgtccggta caccgtcgcc aggccgatcg gctccccggc
     2821 ggagcgcagg ctcgcgtgca gttcctgcgc ggtcacggcg tcgtcgcgct cgcgcagcgc
     2881 ctccacgacg ctgcgccgtt gccgcgtcgg gcgcagcccg accgccagca ggtcc
//
LOCUS       DATMKC010000112         8022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_149126, whole genome shotgun sequence.
ACCESSION   DATMKC010000112 DATMKC010000000
VERSION     DATMKC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149126"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VNU01_03230"
     CDS             <1..216
                     /locus_tag="VNU01_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31664.1"
                     /translation="LPVGAAVLGGAVVVTALAGVRGAVAVAAGVVVAVAIGVWARRRD
                     DRWTTELADAGALVVETIALLATVAVR"
     gene            416..616
                     /gene="infA"
                     /locus_tag="VNU01_03235"
     CDS             416..616
                     /gene="infA"
                     /locus_tag="VNU01_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWH31665.1"
                     /translation="MELEGVVEEALPNTMFRVKLDNGHTVLGHISGKMRKHYIRILPG
                     DRVTVELSPYDLTRGRITYRYK"
     gene            complement(657..1640)
                     /locus_tag="VNU01_03240"
     CDS             complement(657..1640)
                     /locus_tag="VNU01_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HWH31666.1"
                     /translation="MGRVVIAPEPGGPEALVFVERPAPRPGPGELAVRVRATAVNRAD
                     VLQRRGFYPPPPGASDVLGLELAGEVTAVGDRVSGWSVGDELCAVVPSGGYAEEAVVP
                     AEVALPLPPGLDAVSAAAVPEVFTTAFDNLVNRARLAPGETVLVHGGAGGVGSAAIQL
                     AKRLGCTVLATAGSPEKADACVGLGADAAIHYGEEDFAERVRELTGGRGADVVLDVMG
                     AAYLARNLDALAMEGRLAVIGLQGGVMGELNLGLLLAKRLTVLGSTLRPRTVEERAPL
                     TAQLVAQVWPGFADGSLRPVIDRILPLEQAAEAHALMEAGELIGKVVLRVS"
     gene            1795..2163
                     /locus_tag="VNU01_03245"
     CDS             1795..2163
                     /locus_tag="VNU01_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWH31667.1"
                     /translation="MSVQIGDVVTGKVVRLHDFGAFVEVDGETTGLVHISEVHRDFVE
                     NIHAYLTEGQEVQVKVLAIKEDGRIDLSIKRAEPDWEDEVQRRQTVRPDKEFNQRLRK
                     FMHQSDMIQGEVRRRKRARQ"
     gene            2238..3374
                     /locus_tag="VNU01_03250"
     CDS             2238..3374
                     /locus_tag="VNU01_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWH31668.1"
                     /translation="MPDNPEVQAVILAGGKGTRLRPVTAELPKPLVPIANRPLMEHQL
                     RHLAAAGVRDVTLALGYNAAAFAGVEREADELGLRLRILTEPAPLGTGGALRWCLDGG
                     AFDERPLLWLNGDVVASPDVASLLEFHTSRAASLTFWLTSVRDVTDFGVLELDPEGRV
                     QRFLEKPAPEESPSNLVNAGILMLDPQMLSRVPPDTFFSFEKSLLPGLVREREPIYGL
                     FDGGYWMDTGRPRTYLAANRHVLENRVDWEPAGELREGVWEGPGVKRDGAIIIHPTVL
                     GEGAVIEDGANIFGRVVVGRGTVVHEGAQLDSSVLHDDVEIGRDCEIVNSIVCKGARI
                     GDGVVLEHAIVGARAVVGERNVLRGARLWNDIELPRRSLVIDQL"
     gene            3426..3668
                     /locus_tag="VNU01_03255"
     CDS             3426..3668
                     /locus_tag="VNU01_03255"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWH31669.1"
                     /translation="MKTTIVLPDALLAEAKRAAAEEQTTLRALIEEGLREVLERRRRR
                     PAFRLPDAAVDGDGLREEFRGADWAAIRDAAYGTPR"
     gene            3665..4096
                     /locus_tag="VNU01_03260"
     CDS             3665..4096
                     /locus_tag="VNU01_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HWH31670.1"
                     /translation="MIALDTNVLVYAHRADSPFHDQAARVLRDLAEGTANWAIPWPCL
                     HEFFAVATHPRIYDPPSTPAQALAQMEAWCASPTVSLLAEELDHLDVLGRLLRAGDVR
                     GPQVHDARVAAVCLSAGVRELWSVDRDFSRFPQLRVVNPLR"
     gene            4140..4619
                     /locus_tag="VNU01_03265"
     CDS             4140..4619
                     /locus_tag="VNU01_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH31671.1"
                     /translation="MSTVSPFSRTPEADHLVGLLGESRGRIVEALRRTPHSVGALAEL
                     IGVSEVAVRRHLSVLERDGLVTAETVRREGPGRPCAEYHLTDRGRRLFPDQSAQFACE
                     LLEFLEEEHGRSAVLRFFQWRQHRHPGRPGAGLAGLIGEDHQTFTTTTTGDSDGHAS"
     gene            4603..7086
                     /gene="sufB"
                     /locus_tag="VNU01_03270"
     CDS             4603..7086
                     /gene="sufB"
                     /locus_tag="VNU01_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWH31672.1"
                     /translation="MATRAEDLQLGTYKYGWSDPSTSVFVPKKGLSEDVVREISALKD
                     EPQWMLDVRLKAYKQFLARPMPTWGGDLSKIIFDDIYYFIRSTEKQATSWDELPDDIK
                     NTYDKLGIPEAEKQRLVSGVAAQYESEVVYHKVREDLEEQGVLFLDTDSALREHPDLF
                     REHFGKVIPPNDNKFAALNTAVWSGGSFIWIPEGVKVDVPLQAYFRINQQNMGQFERT
                     LIIAEPGSYIHYVEGCLPAGELVSAADGSRVPIEQVRVGDEVLDQRGRPVRVTATQAR
                     DYRGPVYTFVPTSPDNAFTVTAEHPVLVVPRADVAVKRAPRGGWLPEVDSGKLKAAEP
                     RWVEAKDVVEGDFLVFPKPRPALAETVLPLEFARLAGYYLADGSATITNGAKSLQFSL
                     HIDDTESVDEIRRLCKELYQAEGSVLWNEAKHEARVLVYTPAGYEALRAHVGTRSHTK
                     KLSAELMGQDAAFLSALVETYLRGDGCVRERAGALWHRANTTSRTWAHQLQAIMARLG
                     VYATISVDRPAGPREILGRAVSRHALYRVQWTVGGSGPRLVRDAGEHFLVPVKRREVG
                     DFEGLVHNLDVRDPDSYLANGFAVHNCTAPIYSSDSLHSAVVEIIVKPGARVRYTTIQ
                     NWSNNVYNMVTKRAAAYEDATMEWIDGNIGSKLTMKYPAVWLMGRGARGEVLSVAFAG
                     DGQHQDAGAKMVHHAPDTSSQIVSKSVSQGTGRTSYRGLVDIQEGAHRVKSSVVCDAL
                     LLDETARSDTYPYMTIAEDDAQIGHEASVSKIGEEQLFYLQSRGVDEAEAMAMIVRGF
                     IEPISKELPMEYSVELNRLISLNMEGSVG"
     gene            7086..>8022
                     /locus_tag="VNU01_03275"
     CDS             7086..>8022
                     /locus_tag="VNU01_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HWH31673.1"
                     /translation="MAAPRELTEHDVAELSAAYGEPDWLRDVRLAAFKAFSALDWPDN
                     RVEEWRYTDPRRFDLARAILTDAGPADAVATGITATVRDAGGSIRIVDGAVVQATVCA
                     EGAEQGVVVTDLATAARDHADLLRDALGTAVGSDEKFDALNLAAFTGGGFVHVPADVE
                     FAQPIIVTVQATRDGAILPRVVISTGRHAKATVYVDHVGAAAATVVEVVELVAGEGSV
                     VQLVSAQDWGGAVDHVATHRGLTRDNATVKALEVTLGGRTVYVRPDVHLAHGAVVAAA
                     DHVAVVDLQERDGVGLRPVREDEVAVGLVRRRGARV"
BASE COUNT         1196 a         2859 c         2880 g         1087 t
ORIGIN      
        1 ctgccggtcg gcgccgccgt cctgggcggg gcggtggtgg tgacggcgct ggccggcgtg
       61 aggggcgccg tggcggtcgc ggccggcgtc gtggtggcgg tggcgatcgg cgtgtgggcg
      121 cgccgccggg acgatcgctg gacgactgag ctcgccgacg cgggggcatt ggtggtggag
      181 accatcgccc tgctcgcgac ggtcgcggtg cgctagctgg tagggtggat gtcgaaatcc
      241 ccgcgccgtg cggcactccg gcgcgagcgg caaccccggt acggactcca agaggaagag
      301 gctgcacacc cttatggcga gatcacgcag cggacgcacg ccgtcacggc gctacgtcga
      361 cgcggagacc cgcgcgaagc gcgaggcccg gtcgggctcc gagaaggaag ccgccctgga
      421 gctcgagggc gtcgtcgaag aggcgctgcc gaacacgatg ttccgcgtga agctcgacaa
      481 cggccacacg gtgctggggc acatctccgg gaagatgcgc aagcactaca tccgcatcct
      541 gccgggggac cgcgtcaccg tggagctgtc gccctacgac ctgacgcgcg gccgcatcac
      601 ctaccgctac aagtagcgcc gcgcccagcg agcgggttga gcacacatcc ggcgggtcac
      661 gacacgcgca gcaccacctt gccgatcagc tcgcccgcct ccatcagggc atgcgcctcc
      721 gccgcctgct ccagcggcag gatgcggtcg atcaccggcc gcagcgaccc gtccgcgaag
      781 cccggccaca cctgggccac gagctgcgcg gtgagcggcg cgcgctcctc cacggtccgc
      841 gggcgcagcg tcgagcccag cacggtcagc cgcttcgcca gcagcaggcc aaggttgagc
      901 tcgcccatca cgccgccctg caggccgatg accgccaggc gcccctccat ggccagcgcg
      961 tcgaggttgc gcgccaggta cgccgcgccc atgacgtcca gcaccacgtc ggcgccgcgg
     1021 ccgccggtga gctcgcgcac ccgctcggcg aagtcctcct cgccgtagtg gatcgccgcg
     1081 tcggcgccca ggccgacgca cgcgtcggcc ttctccggcg agccggcggt ggccagcacc
     1141 gtgcagccca ggcgcttggc gagctggatc gctgcgctgc cgaccccgcc ggcgccgccg
     1201 tgcaccagca cggtctcgcc cggtgccagc cgcgcgcggt tcaccaggtt gtcgaacgcg
     1261 gtggtgaaca cctcggggac cgcggcggcc gacaccgcgt ccaggcccgg cggcagcggc
     1321 agcgccacct cggccggcac gaccgcctcc tcggcgtagc cgccgctggg gaccaccgcg
     1381 cacagctcgt cgcccacgga ccagccgctg acgcggtcgc ccacggcggt gacctcgccc
     1441 gccagctcca gccccagcac gtcggacgcc ccgggcggcg gcgggtagaa gccgcgccgc
     1501 tggagcacgt cggcgcggtt gacggcggtc gcgcgcacgc gcacggccag ctcgcccggc
     1561 cccggccgcg gcgccgggcg ctccacgaac accagcgcct ccggcccgcc cggctccggc
     1621 gcgatcacga ctctgcccat tgcgtctcct cgtcgtctgc cgtttgccgc gcagtatcgg
     1681 ccgcaaaacc tgggtgtgcg gaatggcgcc tctggcgagg ctggtaccct ggcccaaacc
     1741 aggagcagtg cgaggcggca gccgcgatca gcggcaggcc gcgagaggag agttgtgtcc
     1801 gttcagattg gagatgtcgt caccggcaag gtcgtccgcc tgcacgactt cggcgcgttc
     1861 gtcgaggtcg acggggagac caccgggctg gtccacatca gtgaggtcca ccgcgacttc
     1921 gtggagaaca tccacgccta cctcaccgag ggccaggaag tccaggtcaa ggtgctcgcg
     1981 atcaaggaag acgggcgcat cgacctgtcg atcaagcgag ccgagccgga ctgggaggac
     2041 gaggtccagc ggcgccagac ggtgcggccg gacaaggagt tcaaccagcg gctgcgcaag
     2101 ttcatgcacc agtccgacat gatccagggc gaggtgcggc ggcggaagcg cgcgcggcag
     2161 taaccggcgg cgccggtttg gggcacggcg gccgcgcgga atgactcgcg ggccgccgcg
     2221 acgaaaggcc tccaaacgtg cccgacaacc cggaagtgca ggcagtcatc cttgcggggg
     2281 gcaagggaac ccgcctgcgc cccgtgacgg ccgagctgcc caagccgctg gtgccgatcg
     2341 ccaaccggcc gctgatggag caccagttgc gccacctggc ggccgccggc gtgcgcgacg
     2401 tcacgctcgc gctgggctac aacgccgccg cgttcgcggg cgtggagcgc gaggcggacg
     2461 agctgggcct gcgcctgcgc atcctgacgg agccggcgcc actgggcacg ggcggcgcgc
     2521 tgcgctggtg cctggacggc ggcgcgttcg acgaacggcc gctgctgtgg ctcaacggcg
     2581 acgtggtggc cagccccgac gtcgcctcgc tgctggagtt ccacacctcg cgcgcggcgt
     2641 cgctgacgtt ctggctgacc tcggtccgtg acgtcaccga cttcggcgtg ctggagctgg
     2701 acccggaggg ccgcgtgcag cggttcctgg agaagccggc gccggaggag tcgccgtcca
     2761 acctggtgaa cgccgggatc ctcatgctcg acccgcagat gctgtcgcgc gtgccgccgg
     2821 acacgttctt ctcgttcgag aagagcctgc tgcccggcct cgtgcgcgag cgcgagccca
     2881 tctacgggct gttcgacggc ggctactgga tggacacggg ccgcccgcgc acgtacctgg
     2941 cggccaaccg ccacgtcctg gagaaccgcg tggactggga gcccgccggg gagctgcgcg
     3001 agggcgtgtg ggaggggccg ggggtcaagc gcgacggcgc gatcatcatc catcccaccg
     3061 tgctggggga gggcgcggtg atcgaggacg gcgcgaacat cttcggccgc gtggtggtgg
     3121 gccggggcac cgtcgtgcac gagggggcgc agctggacag ctccgtgctg cacgacgacg
     3181 tcgagatcgg ccgcgactgc gagatcgtga actccatcgt gtgcaagggc gcccgcatcg
     3241 gcgacggcgt ggtcctggag cacgccatcg tcggggcccg cgccgtggtg ggcgagcgca
     3301 acgtgctgcg cggcgcccgc ctgtggaacg acatcgagct gcccaggcgc agcctcgtca
     3361 tcgaccagct ctgacacggc tcgcggcgta gccgtatgta cggctgccat ggtacggtca
     3421 gccgtatgaa gaccacgatc gtgcttcccg atgcgctgct cgccgaggcc aagcgcgccg
     3481 ccgccgagga gcagaccacc ctgcgcgccc tgatcgagga aggcctgcgg gaggtcctgg
     3541 agcggcggcg ccgacggccg gcgttccgcc ttcccgacgc cgcggtggac ggcgacgggc
     3601 tgcgcgagga gttccgcggc gcggactggg cggcgatcag ggatgccgcc tacggcacgc
     3661 cgcggtgatc gccctcgaca cgaacgtcct ggtgtacgcc caccgcgccg actctccgtt
     3721 ccacgaccag gcggcgcgtg tgctgcggga cctggctgag ggcacggcga actgggcgat
     3781 cccgtggccc tgcctgcacg agttcttcgc cgtggcgacg cacccccgga tctacgaccc
     3841 gccgtccacg cccgcgcagg cgctggccca gatggaggcc tggtgcgcgt cgccgacggt
     3901 gtccctgctg gccgaggagc tcgaccacct cgacgtgctg gggcgcctcc tgcgcgccgg
     3961 cgacgtgcgc gggccgcagg tgcacgacgc ccgggtggcg gcagtctgcc tgtccgcggg
     4021 cgtgcgcgag ctgtggtccg tcgaccgcga cttcagccgg tttccccagc tccgggtggt
     4081 gaaccccctg cgctgacggg cgatgtgcag ggtttctcaa cattctgtat gcttatttca
     4141 tgtccaccgt ctcgccgttc tcgcgcacgc cggaggccga ccacctggtc ggcctgctcg
     4201 gcgagtcgcg cgggcgcatc gtcgaggcgc tgcggcgcac gccgcacagc gtcggcgccc
     4261 tggccgagct catcggcgtg tccgaggtgg ccgtgcgccg gcacctgtcg gtgctggagc
     4321 gcgacggcct cgtcaccgcc gagaccgtgc ggcgcgaggg gcccgggcgc ccgtgcgccg
     4381 agtaccacct gaccgacagg ggccgccggc tcttcccgga ccagtccgcg cagttcgcct
     4441 gcgagctgct ggagttcctg gaggaagagc acggccgctc cgcggtgttg cgcttcttcc
     4501 agtggcgcca gcaccgccac cccgggcgtc ccggcgccgg gctcgccggc ctgatcggcg
     4561 aggaccacca gacgttcacg accacgacca caggagacag tgatggccac gcgagctgaa
     4621 gacctccagc tcggcacgta caagtacggc tggagcgatc cctccacgtc ggtcttcgtg
     4681 ccgaagaagg gcctgtccga ggacgtggtg cgcgagatct ccgcgctcaa ggacgagccg
     4741 cagtggatgc tggacgtgcg cctgaaggcc tacaagcagt tcctggcccg gcccatgccg
     4801 acctggggcg gcgacctgtc caagatcatc ttcgacgaca tctactactt catccgctcc
     4861 acggagaagc aggcgacgtc gtgggacgag ctgcccgacg acatcaagaa cacctacgac
     4921 aagctgggca tcccggaggc ggagaagcag cgcctcgtct ccggtgtagc ggctcaatat
     4981 gagtcagagg tcgtctacca caaggtgcgc gaggacctcg aggagcaggg cgtgctcttc
     5041 ctggacaccg acagcgcgct gcgcgagcac cccgacctgt tccgcgagca cttcggcaag
     5101 gtgatcccac ccaacgacaa caagttcgcg gcgctgaaca ccgcggtgtg gtccggcggg
     5161 tccttcatct ggatcccgga gggcgtcaag gtcgacgtcc cgctgcaggc ctacttccgc
     5221 atcaaccagc agaacatggg ccagttcgag cgcacgctga tcatcgccga gcccggcagc
     5281 tacatccact acgtggaggg ctgcttgccc gccggcgagc tcgtgtccgc ggcggacggt
     5341 tcgcgcgtgc cgatcgagca ggtccgggtc ggcgacgagg tgctcgacca gcgcggccgc
     5401 cccgtgcggg tcacggccac gcaggcgagg gactaccgcg ggccggtcta cacctttgtt
     5461 cccacctcgc cggacaacgc cttcacggtc accgccgagc acccggtcct cgtggtgccg
     5521 cgtgccgacg tcgcggtgaa gcgagcgccg cgcggcggat ggctgccgga ggtcgacagc
     5581 ggcaagctca aggcggctga gccgcgctgg gtcgaggcca aggacgtcgt cgagggtgat
     5641 ttcctggtct tccccaagcc caggccggct ctggcggaga cggtcctgcc gctggagttc
     5701 gcccggctgg ccggctacta cctcgcggac ggctcggcga cgatcaccaa cggcgccaag
     5761 tcgctccagt tctccctgca catcgatgac acggagtcgg tcgacgagat ccgccgcctc
     5821 tgcaaggagc tgtaccaggc cgaaggctcc gtgctgtgga acgaggccaa gcacgaggcg
     5881 cgggtgctcg tctacacccc cgccggctac gaggcgctgc gcgcgcacgt gggaacgcgg
     5941 tcgcacacca agaagctgtc ggccgagctg atgggccaag acgcggcgtt cctgtcggca
     6001 ctcgtggaga cgtacttgcg tggcgacggg tgtgtgcgcg agcgcgccgg cgccttgtgg
     6061 catcgcgcga acaccacgtc ccgtacctgg gcgcaccagc ttcaggcgat catggcccgg
     6121 ctcggggtgt acgcgaccat cagcgtcgac cggccggccg gaccccgtga gatcctgggc
     6181 cgcgcggtga gccgccacgc gctgtaccgc gtccagtgga cggtcggcgg cagcggcccc
     6241 cggctcgtcc gcgacgcggg tgagcacttc ctggtgccgg tcaagcggcg tgaggtcggt
     6301 gacttcgagg ggctggtcca caacctcgac gtgcgcgacc ccgacagcta cctggccaac
     6361 gggttcgccg tccacaactg caccgcgccc atctacagct ccgacagcct gcacagcgcg
     6421 gtcgtcgaga tcatcgtcaa gccgggcgcc cgcgtgcgct acacgaccat ccagaactgg
     6481 tcgaacaacg tctacaacat ggtgaccaag cgcgcggcgg cctacgagga cgccaccatg
     6541 gagtggatcg acggcaacat cggctccaag ctcaccatga agtacccggc ggtgtggctg
     6601 atgggccgcg gcgcccgcgg tgaggtgctg tcggtggcgt tcgccggcga cggccagcac
     6661 caggacgccg gggccaagat ggtccaccac gcgcccgaca cgtccagcca gatcgtgtcc
     6721 aagtccgtgt cgcagggcac cggccgcacc agctaccgcg gcctggtgga catccaggag
     6781 ggcgcgcacc gcgtgaagtc cagcgtcgtg tgcgacgcgc tgctgctgga cgagaccgcg
     6841 cgcagcgaca cctacccgta catgaccatc gccgaggacg acgcgcagat cggccacgag
     6901 gccagcgtgt ccaagatcgg cgaggagcag ctgttctacc tgcagtcgcg cggcgtcgac
     6961 gaggccgagg ccatggccat gatcgtgcgc ggcttcatcg agccgatcag caaggagctg
     7021 cccatggagt actccgtgga gctcaaccgc ctgatcagcc tgaacatgga agggtccgtc
     7081 ggctgatggc agcaccaagg gagctcaccg agcacgacgt cgccgagctg tcggccgcct
     7141 acggcgagcc ggactggctc cgcgacgtgc gcctggccgc cttcaaggcg ttcagcgcgc
     7201 tggactggcc ggacaaccgc gtggaggagt ggcgctacac cgacccgcgc cgcttcgacc
     7261 tggcgcgcgc gatcctgacc gacgccgggc cggccgacgc ggtcgccacc ggcatcaccg
     7321 ccaccgtgcg ggacgccggc ggctcgatcc gcatcgtcga cggcgccgtg gtgcaggcca
     7381 ccgtgtgcgc cgagggcgcg gagcagggcg tcgtcgtcac ggacctggcc acggccgcgc
     7441 gcgaccacgc cgacctgctg cgcgacgcgc tgggcaccgc ggtgggcagc gacgagaagt
     7501 tcgacgcgct caacctggcg gcgttcaccg gcggcggctt cgtccacgtg cccgccgacg
     7561 tggagttcgc ccagccgatc atcgtgacgg tgcaggccac ccgcgacggg gcgatcctgc
     7621 cgcgcgtggt gatcagcacc ggccgccacg ccaaggccac cgtgtacgtc gaccacgtcg
     7681 gcgccgcggc ggccaccgtg gtcgaggtcg tggagctggt cgccggcgag ggctccgtgg
     7741 tccagctcgt cagcgcccag gactggggcg gcgcggtcga ccacgtcgcc acgcaccgcg
     7801 gcctcacgcg cgacaacgcc acggtcaagg cgctggaggt caccctgggc gggcgcaccg
     7861 tctatgtgcg cccggacgtg cacctggccc acggagctgt ggtggccgca gcggaccacg
     7921 tcgctgttgt ggatctccag gaacgggatg gagtcggcct tcgccccgtc cgtgaggatg
     7981 aggttgcggt tggtctggtc cgacgccgtg gcgcgcgcgt gc
//
LOCUS       DATMKC010000113        11167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_16738, whole genome shotgun sequence.
ACCESSION   DATMKC010000113 DATMKC010000000
VERSION     DATMKC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16738"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..660)
                     /locus_tag="VNU01_03280"
     CDS             complement(250..660)
                     /locus_tag="VNU01_03280"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HWH31674.1"
                     /translation="MKGLKSTTDYRMASRALVDQVSRGLVAIQDVCDAHPELLRAARN
                     VGAPARRACPICTLADDRAEVPRDEAESLRLVTYVFGDALKRLSGHIVWERRELDELA
                     RSYDSFMAYVVECCLVCGWNHMVESYLMGRRHAV"
     gene            841..1449
                     /locus_tag="VNU01_03285"
     CDS             841..1449
                     /locus_tag="VNU01_03285"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWH31675.1"
                     /translation="MMLELAVLGLLKERAMHGYELRKQLSQKLGLFWTVSFGSLYPTL
                     RRLEKRGAVEKIFPEDSTTRRKQVYRITDTGEREFLELLSEGASSTFEEDKFSLRVAF
                     FRYLKPEMRIRLLERRKAYLQDKLEEGHLALVRAKRGRADGYTLSLMRHGMDTTQADI
                     AWLDELINAESAQLAEADPAKPEPAPPPEPRVEDSPETLKTT"
     gene            1491..2624
                     /locus_tag="VNU01_03290"
     CDS             1491..2624
                     /locus_tag="VNU01_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWH31676.1"
                     /translation="MVSDGRTIRVAIVGVGNCASSLVQGVHYYRDAEPGTDVPGLMHV
                     ELGGYHVRDLEFVAAFDVDAKKVGRDLAEAIVAPPNNTIQFAEVPPIGVEVQRGPTLD
                     GFGEFYREVCEESDQPAVDVAQVLRDTRAEVLVCYLPVGSEQAARFYAAECLKAGVAF
                     VNALPVFIASDPEWAQRFTDAGVPIIGDDIKSQVGATITHRILTRLFEDRGVSIDRTY
                     QLNFGGNMDFMNMLERSRLKSKKVSKTQSVTSQMDAPIAKDAIHIGPSDHVPWLEDRK
                     WAYIRMEGRNFGDVPLNIELKLEVWDSPNSAGVVIDAVRCAKLALDRGIGGPLLAPSS
                     YFMKSPPVQFHDDVCHKMVEEFIAGPVEDDIVGLVEEAEARAG"
     gene            complement(2629..2964)
                     /gene="sodX"
                     /locus_tag="VNU01_03295"
     CDS             complement(2629..2964)
                     /gene="sodX"
                     /locus_tag="VNU01_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HWH31677.1"
                     /translation="MPRGATKRWWPLTRVTVTGASMAPHLLPGDRLVVLRTRRVRPGD
                     IAVAADPRDPSRPLVKRVADIQGAGVVLVGDNAAASTDSRAFGPVPRERLRGRAVYRY
                     APSERAGLL"
     gene            3029..3448
                     /gene="sodN"
                     /locus_tag="VNU01_03300"
     CDS             3029..3448
                     /gene="sodN"
                     /locus_tag="VNU01_03300"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HWH31678.1"
                     /translation="MSLIARLFAAADRVAEPRTVHAHCDLPCGVYDPAQARIEAESVR
                     NCQQKYTENDDPVFRQRAVLIKEERAEMVKHHLWVLWTDYFKPNHLEQYPQLHELFWK
                     ATKAAGEAKKSQDPAQGEELLGLVDQIAEIFWETKKA"
     gene            complement(3528..3923)
                     /locus_tag="VNU01_03305"
     CDS             complement(3528..3923)
                     /locus_tag="VNU01_03305"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWH31679.1"
                     /translation="MGRPGIVTFEPMALPEHELLARIFRTLGDSTRLRMIERLLEVGE
                     ASQSELIELVGATQSRASEHLSCLAWCGIVRVQRDGRTARYCLVGEHPEQFLKLARSF
                     LRDNPNAVGHCTILEEEDGQPQSTSMAHA"
     gene            4201..4677
                     /locus_tag="VNU01_03310"
     CDS             4201..4677
                     /locus_tag="VNU01_03310"
                     /inference="COORDINATES: protein motif:HMM:NF033632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HWH31680.1"
                     /translation="METAELIRQWRTAVRVSHRAHADAAARWERAGRLVGVPAIGLAS
                     LVGTVILATIEASPGRTARAFAGLAAVAAAALVSLQTFLGHSDRAQRHREAALRYAWL
                     RRELDDLAAGGERDAKAKRRLEDIRARWAEVDAMAPAIPARQLARARDLVLGTRES"
     gene            complement(4697..4927)
                     /locus_tag="VNU01_03315"
     CDS             complement(4697..4927)
                     /locus_tag="VNU01_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31681.1"
                     /translation="MGTGPDEAKRGVLEEAKGMAKELGGEVTGNERMRQEGEAQQEKA
                     DAHRDAARAEARAEKERAQAQRHEAEQRANQQ"
     gene            complement(5090..5854)
                     /locus_tag="VNU01_03320"
     CDS             complement(5090..5854)
                     /locus_tag="VNU01_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31682.1"
                     /translation="MAWTDREGNPVEVPEPPPSGSYYTEVGDFQGAAYERNAFALGTR
                     QEVEFLWDALRLRPGEVLLDVGCGTGRHTRAFAERGLRSVGVDISPGLLRAAAAHGTT
                     AAFVLADARAIPLADAAVDAVVCLCQGGFGITPGGDRAILREIARVLRPGGRLALTAF
                     SLVFAARYLAPEDAIDTSRGLVWSPAEVRGADDERRRFDLWTSCYSAGELRDMLLTSG
                     FDLESLSGVEPGAYGRHAPTIAAPELLALAVRRPQL"
     gene            complement(5855..7303)
                     /locus_tag="VNU01_03325"
     CDS             complement(5855..7303)
                     /locus_tag="VNU01_03325"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR02692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWH31683.1"
                     /translation="MASSLTAAQSRQLAEMVAVYPVALELGERFAAAGHELYLVGGTV
                     RDTLLGRGDQPDLDFATSARPEDTERLLKGWADAVWLTGAAFGTVSCQKADAKGDWTL
                     EITTFRSDVYVPGSRHPEVTYGDAIEEDLSRRDFTVNAMAVRVPDLQFVDPFGGLKDL
                     HAKVLRTPVAPQQSFADDPLRMVRLARFAAVLGATPDAAARSAAADMAGQLDAVSRER
                     IRIELDKLLVAEFAGAGLDLLVETGLADRFLPELPALRLERDPLHHHKDVYRHTLAVV
                     ENCPRHDVVLRLAALLHDIGKPATREFHPDGKVSFHHHEVVGARMAEKRLKELKYPNE
                     VVQQVRDLVFLHLRFHGYGEQAWTDSAVRRYARDAGTPQQLWRLNQLTRADITTQNRA
                     KARRLKAAVDELEQRIALLEEQEELSKLRPALDGNEIMAHLRIPPGPLVGQAWNMLLE
                     ARLDRGPMTAEEAYALLDEWARTQGVPPDREP"
     gene            complement(7337..8410)
                     /locus_tag="VNU01_03330"
     CDS             complement(7337..8410)
                     /locus_tag="VNU01_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWH31684.1"
                     /translation="MDLHDYLRYTADKGGSDLHLKAGGPAYVRIDGTLSPCNDLPALT
                     AADTERFARQAMSDELWERFSSGAEADFAYAAEGLGRFRVAVFRQRGEVGLVLRRVLP
                     GAQRFDELNLPPAVRKLAEEHRGLVLVTGPTGSGKTTTTAAMLSHINATRPCHIVTVE
                     DPIEVLHHDDLAIVDQREVGVDTASFSSALRSVARQDPDVIFIGEMRDEETVSAALQA
                     AETGHMVISTLHTTNAKETVNRILDIFPAHQQNQVRISLAHALQGIVCQRLLPKAGGG
                     RIAAIECLVNTTRVREFILDPDKTSMIEDAVAEGAYYGMQTFDQHLLELFKSGQVTLK
                     DAMDAATSPHDFRIMVRNAGLEA"
     gene            8498..8989
                     /locus_tag="VNU01_03335"
     CDS             8498..8989
                     /locus_tag="VNU01_03335"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWH31685.1"
                     /translation="MARYPTQEAVSAGGLVFSDRPDGRWVVLISRRSAAGALHWTLPK
                     GRLEDGESSEQAAVREVAEETGFACEITQPLGTIDYWFVWRPDKVRYHKFVHYFLMRY
                     LGPAPAPRDDEAEDVVWLPVKEALTRLTHANERDLLARALEAHEQEAAAVTGTVADGA
                     REA"
     gene            8986..11118
                     /locus_tag="VNU01_03340"
     CDS             8986..11118
                     /locus_tag="VNU01_03340"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HWH31686.1"
                     /translation="MRRLRPRRSAPAVVALALAVALPGGGAAAQSERGLDGDETRPGR
                     GGVALEVADFTGAVSPEGSFRLRVRVANRTRSAEESLRIVATFHRRAFHRLDFARAVD
                     EGRVGDVYTSVSQELTSLEGRRVRTVEIRRSAEELGFGRGEDHQGVYPLVIRLLREDT
                     TIQTVRTAVVVVSSEVAEPVRAALVVPLDAPPALLPDGVLRPDVLRQDLAPRGRLRAL
                     LTDLGERPDVPVTFAVDARLLDDVADFADGYRSLGADGPVEHDADSSLARASRGFLSE
                     LGRVLPQPHVETLALPYGPADLSALVRGGMSQEAGRLISEGAARVEAHTGARPSPNVL
                     LPADDLSPAALSEVVSAGADGVVLDGDTLEPGAAASTSSPSPARLLRTASGTTVTAAI
                     PDPWLTELLADEEDLEELDRPVAIQRLIAETAAVYFERPFATQPRGLLLLAPLRWSPP
                     GDFAGGLAGALRAAPWLQPVTLTTLLDEVEAPEGSPARVEYPDTARARELSPAYVTQL
                     RASRRALGSLAGVLAADTATPSRYDRMLLAAASVWYREDPAAGLALVREVLGTVDELY
                     DAVRVADSPTVLLPGIEGQIPVTVENRSPIPLRLAIRMQSQRFAFEDGPTRVVVVGAG
                     QTHTVAFRAHDRSPGGTSPIRVVVADADGQLQLAEGTLVVRSTAVSAAALAVVVGAGL
                     FLLGWWLRTFRRRRSGRRADQAAAPDAA"
BASE COUNT         1573 a         3993 c         4038 g         1563 t
ORIGIN      
        1 ctcatgggcc gcggcgtgcc gcgcggccaa ccgcaagggc gccgaaaggc cgggcagcgg
       61 caaggagcgt cgcccgcggc gccgcccgag gagcgaacga gcggcccaag gacctcatgg
      121 gtcgcggcgg catggcagaa aagaacaggc gccagaacgg gcgcggggcg ccgcccgagg
      181 agcgaacgat tcgcgccgaa ggcgcgactg agcgacgagg gatggcgccc gcgcccgacg
      241 gcgccaggct caaacggcgt ggcgccgacc catgaggtac gactccacca tgtggttcca
      301 cccgcacacc aggcagcact ccaccacgta ggccatgaag gagtcgtagc tgcgcgcgag
      361 ctcgtccagc tcccgccgct cccacacgat gtggccggac aggcgcttca gcgcgtcgcc
      421 gaagacgtag gtgaccaggc gcagggactc cgcctcgtcg cgcgggacct cggcgcggtc
      481 gtcggccagg gtgcagatgg ggcaagcgcg acgggccggc gcgcccacgt tgcgggcggc
      541 gcgcagcagt tccggatggg cgtcgcagac gtcctggatg gcgacgagcc cgcgcgagac
      601 ctgatcgacc agcgctctgc tggccatccg gtagtcggtg gtgctcttca ggcccttcat
      661 cgcgtgtcga gtgtaggtcg gcacgttcgc ccgggcgctg aaaagctcgt ggcgggtgct
      721 tgcgccgagc gcgcgctgat gtagcatcga gatatatcgc gctgatacat cgatgggata
      781 tgtccccgat acgtcagttg ccgtgcgaag accacccgcc gaagtgagga acgccgcccc
      841 atgatgctgg aactggccgt tctcgggctc ctgaaggagc gcgcgatgca cggctatgag
      901 ctgcgcaagc agctcagcca gaagcttggc ctgttctgga ccgtgtcgtt cggctcgctg
      961 tacccgaccc tgcggcggct ggagaagcgc ggcgcggtgg agaagatctt ccccgaggac
     1021 tcgaccacgc ggcgcaagca ggtgtaccgc atcacggaca cgggcgagcg ggagttcctg
     1081 gagctgctga gtgagggcgc cagcagcacc ttcgaggagg acaagttctc cttgcgggtc
     1141 gccttcttcc gctacctgaa gccggagatg cgcatccgcc tgctggagcg ccgcaaggcg
     1201 tacctccagg acaagctgga ggaaggccac ctggccctgg tgcgggccaa gcgcggccgc
     1261 gccgacggct acaccctgtc gctgatgcgc cacggcatgg acacgaccca ggcggacatc
     1321 gcctggctgg acgagttgat caacgcggag tccgcccagc tcgctgaggc ggacccggcc
     1381 aagcccgagc cggccccccc gccggagcca cgcgtcgagg acagccccga gacgctcaag
     1441 acgacctgac gcgcggacct gcgcgcgagg cacacctaca aggagaaccg atggtcagcg
     1501 atggccggac gattcgagtc gcgatcgtgg gcgtgggcaa ctgcgccagc tcgctggtgc
     1561 agggcgtcca ctactaccgg gacgccgagc cgggcacgga cgtgcctggc ctgatgcacg
     1621 tggagctggg cggctaccac gtgcgggacc tggagttcgt ggccgccttc gacgtggacg
     1681 ccaagaaggt gggccgcgac ctggcggagg ccatcgtcgc gccgcccaac aacaccatcc
     1741 agttcgccga ggtgccgccg atcggcgtgg aggtgcagcg cggccccacc ctggacggct
     1801 tcggcgagtt ctaccgcgag gtgtgcgagg agtccgacca gcccgcggtc gacgtcgccc
     1861 aggtgctgcg cgacacgcgc gccgaggtgc tggtgtgcta cctgccggtg ggatcggagc
     1921 aggcggcgcg cttctacgcc gcggagtgcc tgaaggccgg tgtcgcgttc gtcaacgcgc
     1981 tgcccgtctt catcgccagc gacccggagt gggcgcagcg cttcaccgac gccggcgtgc
     2041 cgatcatcgg cgacgacatc aagagccagg tgggcgcgac gatcacccac cgcatcctca
     2101 cccgcctgtt cgaggaccgg ggcgtgtcca tcgaccgcac ctaccagctc aacttcggcg
     2161 gcaacatgga cttcatgaac atgctggagc gcagccgtct gaagtccaag aaggtctcca
     2221 agacgcagtc cgtgacgtcg cagatggacg cgcccatcgc caaggacgcc atccacatcg
     2281 gcccctccga ccacgtgccg tggctggagg accgcaagtg ggcctacatc cgcatggagg
     2341 gccgcaactt cggcgacgtg ccgctgaaca tcgaactgaa gctggaggtc tgggactccc
     2401 ccaactccgc cggcgtcgtc atcgacgcgg tgcgctgcgc caagctggcg ctggaccgcg
     2461 gcatcggcgg cccgctgctg gccccgtcca gctacttcat gaagtccccg ccggtgcagt
     2521 tccacgacga tgtctgccac aagatggtgg aggagttcat cgccggcccg gtcgaggacg
     2581 acatcgtggg gctggtcgaa gaggccgagg cgcgcgccgg ctaggcgctc acaggaggcc
     2641 ggctctctcc gagggggcgt agcggtacac cgcgcgcccc ctcagccgtt cccggggcac
     2701 ggggccgaag gcccggctgt cggtgctggc ggcggcgttg tcccccacga ggacgacgcc
     2761 cgcgccctgg atgtcggcca cccgcttgac cagcggccgc gacgggtctc gcggatcggc
     2821 cgccacggcg atgtcgccgg gccgcacgcg gcgggtgcgc agcaccacca ggcggtcgcc
     2881 gggcagcagg tgcggcgcca tgctggcgcc cgtcacggtc acccgggtga gcggccacca
     2941 ccgcttggtt gcgcctcggg gcatggggat agagtacgcc gggaatcgca aaggactcgt
     3001 tcacgccaaa cccgaaggag cgcttgacat gtcgctgatc gctcgcctgt tcgcggccgc
     3061 cgaccgcgtg gctgagccgc gcacggtgca cgcccactgc gacctcccgt gcggggtgta
     3121 cgacccggcg caggcccgga tcgaggcgga gtcggtgcgc aactgccagc agaagtacac
     3181 cgagaacgac gacccggtgt tccgccagcg cgccgtgctc atcaaggagg agcgggccga
     3241 aatggtgaag caccacctgt gggtgctgtg gaccgattac ttcaagccca accacctgga
     3301 gcagtacccg cagctgcacg agctgttctg gaaggccacc aaggccgccg gcgaggccaa
     3361 gaagagccag gacccggcgc agggcgagga gctgctgggc ctggtcgacc agatcgccga
     3421 gatcttctgg gagaccaaga aggcctgatc gccgagcgga acgcagcggc ccccgggaga
     3481 ccgggggccg ttttttcgcc tgaagcgcgc tggtgggggt tcgcggttca ggcgtgcgcc
     3541 atgctcgtgg actgaggttg gccgtcctcc tcctcgagga tcgtgcagtg cccaacggca
     3601 ttggggttgt ctcgcaagaa gctgcgcgcc agcttcagga actgctcggg gtgctcgccg
     3661 accaggcagt agcgggccgt gcggccatcg cgttgcacgc gcacgatccc gcaccaggcc
     3721 aggcaggaca ggtgctcgga tgcccgggac tgcgtcgcgc cgaccagttc gatgagctcg
     3781 gactgcgacg cctcgcccac ctcgagcagc cgctcgatca tgcgcagtcg cgtcgagtca
     3841 ccgagtgtcc ggaagatgcg tgcaaggagc tcgtgctccg gcaacgccat cggctcgaac
     3901 gtgacgattc ctggccggcc catcttgtac tcccccgtgt caaccctcgc gaggatgtgc
     3961 gccttacggt aaggcacact gccgcggact gccggaatat cggataaaat agcccgatat
     4021 gcccggcccc ccgtggccgt tactcttcgg aacctaccat ccggtagcta ccccggaaag
     4081 caagcggttc ggcgcttact caccccactc aacggggcca gggccattcc ctttcgcact
     4141 ttcttcgcgc cggctggaat ttcttctggc accggcacgc ggctggcatc atccgcccgg
     4201 atggaaaccg cggaactgat caggcagtgg cgcacggcgg tgcgcgtcag ccaccgcgcc
     4261 cacgccgacg ccgcggcgcg ctgggagcgg gccgggcggc tggtcggtgt gccggcgatc
     4321 ggcctggcct cgctggtcgg cacggtcatc ctggcgacca tcgaggcctc acccggccgc
     4381 acggcccgcg cgttcgcggg gctggccgcg gtggcggccg cggcactggt gtcgctgcag
     4441 acgttcctgg gacacagcga ccgggcccag cgccaccggg aagcggcgct gcgctacgcg
     4501 tggctgcggc gcgagctgga cgacctggcg gccggcggcg aacgcgacgc caaggcgaag
     4561 cggcgcctgg aggacatccg cgcgcgctgg gcggaggtcg acgccatggc gcccgcgatc
     4621 ccggcgcgcc aactcgcgcg cgcccgggat ctggtcctgg gcacgcgcga gagctgatcc
     4681 ggccatccgt ggccgtctac tgctggttgg cgcgctgctc cgcctcgtgg cgttgggctt
     4741 gcgcgcgctc cttctcggcc cgcgcctcgg cgcgggcggc gtcacggtgg gcgtcggcct
     4801 tctcctgctg cgcctcgccc tcctggcgca tgcgctcgtt gccggtcacc tcgccaccca
     4861 gctccttggc catgcccttg gcctcttcca gtacgccgcg cttcgcttcg tccgggccag
     4921 ttcccatcgg tgcacctccc tcgatgtcga cgaatgccga ccgtggcggc ctacccgggc
     4981 gccgcatgcg cgaagcccgc ccaccgggcg gaaagtccgc cgcgtgcggc cgacgacgtg
     5041 gactggaagt gatttccatg acatcgaggg cgagtatcaa caatgttgtt cacagttgtg
     5101 gacgacggac cgcgagggcg aggagctcgg gcgcggcgat cgtcggcgca tggcgcccgt
     5161 aggcgcccgg ctcgacaccg gaaagacttt ccagatcgaa gccgctggtc agcagcatgt
     5221 cccgtagttc cccggcgctg tagcagctcg tccacagatc gaagcggcgg cgttcgtcgt
     5281 ccgcgccgcg cacctcggcc ggcgaccaca ccaggccgcg cgaggtgtcg atcgcgtctt
     5341 ccggcgccag atagcgcgcc gcgaagacga gcgagaacgc ggtcagcgcc aggcggccac
     5401 cagggcgcag cacccgtgcg atctcccgga ggatcgcgcg gtcgcctccg ggcgtgatgc
     5461 cgaagccgcc ctggcacagg cagacgaccg cgtccacggc ggcgtcggca agcgggatgg
     5521 cgcgcgcgtc ggcgagcacg aacgcggccg tggtgccatg cgccgcggcg gcgcgcagca
     5581 gccccggcga gatgtcgacg ccgaccgacc ggagcccacg ttcggcgaac gcccgcgtgt
     5641 gacgcccggt gccgcagccg acgtccagca ggacctcgcc agggcgcagg cgcagcgcgt
     5701 cccacaggaa ctccacctcc tgccgggtgc ccagcgcgaa cgcgttgcgc tcgtacgccg
     5761 ctccctggaa gtcgccgacc tcggtgtagt acgagcccga cggcgggggc tccggcacct
     5821 cgacgggatt gccctcccgg tccgtccagg ccacctaggg ctcccggtcc ggcgggacgc
     5881 cttgggtccg cgcccactcg tccagcagcg cgtacgcctc ctcggcggtc atgggccccc
     5941 gatccaggcg cgcctccagc agcatgttcc acgcttggcc gaccaggggc ccggggggga
     6001 tgcggaggtg ggccatgatc tcgttgccgt ccagcgccgg gcgcagcttg gagagctcct
     6061 cctgctcctc caacagcgcg atgcgctgct ccagttcgtc caccgcggcc ttcaggcgcc
     6121 gcgccttggc gcggttctgc gtggtgatgt cggcacgcgt cagctggttc aggcgccaca
     6181 gctgctgggg cgtgccggcg tcgcgcgcgt agcggcgcac cgccgagtcg gtccacgcct
     6241 gctcgccgta gccgtggaag cgcaggtgca aaaacaccag gtcgcggacc tgctgcacga
     6301 cctcgttggg gtacttcagc tccttcaggc gcttttcggc catgcgcgcc ccgaccacct
     6361 cgtggtggtg gaagctgacc ttgccgtccg ggtggaactc ccgggtcgcg ggcttgccga
     6421 tgtcgtggag cagggccgcc aggcgcagga cgacgtcgtg ccgcgggcag ttctccacga
     6481 ccgccagcgt gtggcggtac acgtccttgt ggtggtgcag cggatcacgt tccagacgca
     6541 gcgccggcaa ctcgggcagg aaccggtccg cgagccccgt ctccaccagg agatcaaggc
     6601 ccgcgccggc gaactccgcc acgagcagct tgtccagctc gatgcggatg cgctcccggg
     6661 agaccgcgtc cagctggccg gccatgtccg cggccgccga ccgggcggcg gcgtcgggcg
     6721 tcgcgcccag gaccgcggcg aagcgcgcca ggcgcaccat gcgcagcgga tcgtccgcga
     6781 agctctgctg cggcgccacc ggcgtgcgca gcaccttggc gtgcaggtcc ttcaggccgc
     6841 cgaacgggtc gacgaactgc aggtccggca cgcgcacggc catggcgttg accgtgaagt
     6901 cccgacggga gaggtcctcc tcgatcgcgt cgccgtaggt gacctcggga tgccgggaac
     6961 ccgggacgta gacgtccgag cggaatgtcg tgatctccag cgtccagtcg cccttcgcgt
     7021 ccgccttctg gcagctgacg gtgccgaacg ccgcgccagt cagccagacc gcgtcggccc
     7081 agcccttgag cagcctctcg gtgtcctccg ggcgggcgga ggtcgcgaag tccaggtccg
     7141 gctggtcgcc gcggcccagg agcgtgtcgc gcacggtccc gccgaccagg tacagctcgt
     7201 ggccggcggc ggcgaagcgt tccccgagct ccagcgccac cgggtagaca gcgaccatct
     7261 cggcgagctg ccgcgactgg gcagcggtga gtgaagaggc catgacggcc tcatcctagg
     7321 cggagaccgg tggacctcag gcttccaggc cggcgttgcg caccatgatg cggaagtcgt
     7381 gcgggctggt ggccgcgtcc atggcgtcct tgagggtcac ctggccggac ttgaacagct
     7441 ccagcaggtg ctggtcgaaa gtctgcatgc cgtagtaggc gccctcggcc acggcgtcct
     7501 cgatcatcga cgtcttgtcg gggtccagga tgaactcgcg cacgcgcgtg gtgttcacca
     7561 ggcactcgat cgcggcgatg cggccgccgc cggccttggg cagcagccgc tggcagacga
     7621 tgccctggag cgcgtgggcc agcgagatcc tgacctggtt ctgctggtgg gccgggaaga
     7681 tgtccaggat gcggttgacg gtctccttgg cgttggtggt gtgcagcgtc gagatcacca
     7741 tgtgcccggt ctcggcggct tgcagcgccg cggacaccgt ctcctcgtcg cgcatctcgc
     7801 cgatgaagat cacgtccggg tcctggcggg ccacggagcg cagcgcggag gagaacgacg
     7861 cggtgtccac gccgacctcg cgctggtcca cgatcgccag gtcgtcgtgg tgcagcacct
     7921 cgatggggtc ctccaccgtg acgatgtgac agggccgcgt ggcgttgatg tggctgagca
     7981 tggccgcggt ggtggtggtc ttgccggagc cggtggggcc ggtgaccagc accaggccgc
     8041 ggtgctcctc cgccagcttg cgcaccgccg gcggcaggtt cagctcgtcg aaccgctggg
     8101 cgccgggcag gacgcgccgc agcaccagcc ccacctctcc gcgctggcgg aacaccgcca
     8161 cgcggaagcg gcccagtccc tccgccgcgt aggcgaagtc cgcctcggcc ccggacgaga
     8221 agcgctccca cagctcgtcg ctcatcgcct ggcgcgcgaa gcgctccgtg tccgccgccg
     8281 tcagggccgg caagtcgttg cacggcgaca gcgtgccgtc gatgcggacg tacgcgggac
     8341 cgcctgcctt gaggtgcagg tccgagccgc ctttgtccgc cgtgtagcgc aggtagtcgt
     8401 ggagatccac gagttgggtg acctttcggg atcgagggca aacactgttc atttcggcgg
     8461 gcgcgccttc gggcttgacc cgcactatcc tccgcgcatg gcccggtatc ccacccagga
     8521 ggcggtgtcc gccggcgggc tggtcttctc agaccgtccc gacgggcgct gggtcgtgct
     8581 catctcccgc cgcagcgccg cgggggcgct gcactggacc ctgccgaagg gccggctgga
     8641 ggacggcgag tcctccgagc aggcggccgt gcgggaggtg gcggaggaga ccggcttcgc
     8701 ctgcgagatc acccagcccc tgggcaccat cgactactgg ttcgtctggc gcccggacaa
     8761 ggtccggtac cacaagttcg tgcactactt cctcatgcgc tacctggggc cggcgcccgc
     8821 gccgcgcgac gacgaggcgg aggacgtggt gtggctgccg gtgaaggagg cgctgacgcg
     8881 gctgacgcac gcgaacgagc gggacctgct ggcgcgcgcg ctcgaagccc acgagcagga
     8941 ggccgcggcc gtcaccggca cggtcgccga cggggcgcgg gaggcgtgag gcgcctgagg
     9001 ccccggcgca gcgcgccggc cgtcgtggcg ctggcgctgg ccgtcgcgct gccgggcggc
     9061 ggtgccgcgg cgcagtcgga acgcggcttg gacggggacg agacgcggcc gggccgcggc
     9121 ggcgtggccc tggaggtcgc cgacttcacc ggagcggtca gccccgaggg cagcttccgc
     9181 ctgcgggtgc gggtggcgaa ccggacccgg tcggccgagg agagcttgcg catcgtggcc
     9241 acgttccacc ggcgcgcctt ccaccgtctg gacttcgccc gggccgtgga cgaaggccgc
     9301 gtcggggacg tctacaccag cgtgagccag gagctcacct ccctggaggg ccggcgcgtg
     9361 cgcacggtgg agatccgccg cagcgccgag gagctgggct tcggccgcgg cgaggaccat
     9421 cagggcgtgt accccctggt gatccgcctg ctgcgggagg acaccaccat ccagacggtg
     9481 cgcaccgccg tggtggtggt gagcagcgag gtcgccgagc cggtgcgcgc cgcgctcgtg
     9541 gtgcccctgg acgcccctcc cgccctgctt ccggacggcg tgctgcgacc cgacgtgctg
     9601 cggcaggacc tggccccgcg cggacgcctg cgcgcgctgc tgaccgacct gggggagcgc
     9661 cccgacgtcc cggtcacgtt cgccgtcgac gcgcgcctgc tcgacgacgt cgccgacttc
     9721 gccgacggct accggtccct gggcgccgac ggccccgtgg agcacgacgc ggactccagc
     9781 ctcgcgcggg cctcgcgcgg tttcctgtcc gagctcggcc gcgtgctgcc gcagccccac
     9841 gtcgagacgc tcgcgctgcc gtacggcccg gcggacctgt ccgccctggt gcgcggcggg
     9901 atgagccagg aggccggccg gctgatcagc gagggagcgg cccgcgtgga ggcccacacg
     9961 ggcgcgcgcc cctcgccgaa cgtcctgctg ccggcggacg acctcagccc cgccgcgctg
    10021 tcggaggtgg tcagcgcggg cgcggacggc gtcgtcctcg acggggacac gctggagccc
    10081 ggcgccgccg cctccacgtc ctcgccgtcg cccgcgcgcc tcctgcgcac cgcctcgggg
    10141 acgaccgtca cggcggcgat ccccgacccg tggctcaccg agctgctggc cgacgaggag
    10201 gacctcgagg agctggaccg gcccgtggcc atccagcgcc tgatcgccga gaccgcggcg
    10261 gtctatttcg agcgcccgtt cgccacccag cctcgcggcc tgctgctgct ggcgccgctg
    10321 cgctggtcgc cgccggggga cttcgccggc ggcctggcgg gcgcgctgcg cgccgccccg
    10381 tggctgcagc ccgtcacgct caccacgctg ctcgacgagg tcgaggcgcc ggagggctcg
    10441 cccgcccgcg tcgagtaccc ggacacggcc cgggcccggg agctgtcgcc ggcgtacgtg
    10501 acgcagctgc gcgcgtcgcg ccgggcgctc ggctcgctcg ccggcgtgct ggccgccgac
    10561 accgccacgc cgtcccgcta cgaccgcatg ctcctggccg ccgcatcggt gtggtaccgc
    10621 gaggacccgg cggccggcct cgcgctcgtc cgcgaggtgc tgggaaccgt cgacgagctg
    10681 tacgacgccg tgcgcgtcgc cgacagcccc accgtgctgc tgccgggcat cgaagggcag
    10741 atccccgtca cggtggagaa ccgctctccc atcccgctgc gcctggcgat ccgcatgcag
    10801 agccagcgct tcgccttcga ggacggtccc acccgcgtcg tggtcgtggg cgccgggcag
    10861 acgcacaccg tggcgttccg ggcgcacgac cgctcacccg gcggcacctc ccccatccgc
    10921 gtcgtggtgg ccgacgccga cgggcagctg cagctcgcgg agggcacgct ggtggtgcgc
    10981 tccacggccg tgtcggcggc ggcgctggcc gtggtggtcg gcgccgggct gttcctgctg
    11041 ggctggtggc tgcgcacctt ccggcgccgc cgctcgggcc ggcgcgccga tcaggccgcc
    11101 gcgccggacg cggcatgagc gggccggccg accccgtgac gggaccgggc agcgccccgg
    11161 ccgggca
//
LOCUS       DATMKC010000114        15743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_266997, whole genome shotgun sequence.
ACCESSION   DATMKC010000114 DATMKC010000000
VERSION     DATMKC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266997"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VNU01_03345"
     CDS             complement(<1..627)
                     /locus_tag="VNU01_03345"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWH31687.1"
                     /translation="MWRGLLLGEDSTLKRLRTVFNRLPGTPRCKVCAAPFRGPARMVT
                     RIAMHGRSPENPLMCGVCMWSLRRRPGGAEVPGSVLFADVRGSTGLAEGRTAAQYHRL
                     LQRFYALAATAIERHRGILDKFLGDGVMALYIPLTSGDAHARAAVGTARDLLAALRRA
                     DGDLAALGVGCGVHTGDMFVGALGTDDRVDFSALGDTVNIAARLGSIAG"
     gene            complement(796..1308)
                     /locus_tag="VNU01_03350"
     CDS             complement(796..1308)
                     /locus_tag="VNU01_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31688.1"
                     /translation="MAGWLDAHATELAAGGALALALVGSVLAVLLARMLRQGRRALLG
                     AQPVLRATRIVPGPAGGLRLEVGIENMAAWAAQDVSVGATVEGRPLEGGLTGASAFGV
                     SPDGATAHGLRASFSWPVGVLDAAVEVRWSWRDGAGRHRAAWRGHLRTPAGGLPGRAA
                     PTAPRGAPRA"
     gene            1383..1455
                     /locus_tag="VNU01_03355"
     tRNA            1383..1455
                     /locus_tag="VNU01_03355"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1416..1418,aa:Ala,seq:ggc)
     gene            1637..2656
                     /locus_tag="VNU01_03360"
     CDS             1637..2656
                     /locus_tag="VNU01_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31689.1"
                     /translation="MADDLSDQGQRHARHHQQADVAVAQVVRGGVRDARDLRRAEHRA
                     AEPGRRERGVDDRRERCACVCGRHRLQERQGLRHEGHPPPGRARLGPAHTQTRAAHVD
                     VRPPQALELAHAQAGLLEQVHHRVPLGGHRAAQGEEVGLRGGLRDLTHGARHAHRAVP
                     GRVRAVEVGEVEHLLDDARVHRDRLRRQLTLRVHPRLHVPGTDLAHLHAPERPQPAHG
                     LAVVRVSRRAHVDARVRELLAHLGERACARLRLCRDVRECAHVRHAHVRKLPRYPVTA
                     RTRFAQRAERAAVHCARLAAADAVLDVVAHPCARGAPLDPDARHEKSEARGACARRST
                     RAHAS"
     gene            complement(2633..2770)
                     /locus_tag="VNU01_03365"
     CDS             complement(2633..2770)
                     /locus_tag="VNU01_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31690.1"
                     /translation="MDVERLFRELPVVSLPGSRKVYVRRDDLEAYLRTHTYDSGRVRP
                     C"
     gene            complement(3466..7689)
                     /locus_tag="VNU01_03370"
     CDS             complement(3466..7689)
                     /locus_tag="VNU01_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31691.1"
                     /translation="MTVTQIERVWVRPTGSLSYVDDGFVPDPDGPLGAFAPDVVKLPE
                     STGGTCLMLIGEPGLGKSTALRADAQKTASLARSGTDHLLTVNLGSTRQEAKLERNIF
                     GSTAWQSWLSATSGVLHVFLDGLDEALLRVDTVASLLIEGLAEAPIDRLSLRITCRSA
                     DRQLALEDFLKDRFRPNGFEIYELLPLRRREIAEAARGRGLDGDSFVTEVIDRRLQAL
                     AMSPMTLKMLLDQRCDGGPLKENRAELYEEACLRWCEEPNPQRPREDSRRLSVGERLA
                     VATRIAAATMLSGRSSIRLDERSAGPDEVALNDLAGGQEIEPLRAIHDAFAVGPVEVH
                     ETLRTGLFSARGVECVGFAHPTVAEYLAARYLAYSSLSDAQIDDLLFSETGRGYRVIP
                     QLSEIAAWLASQSPPFVQRLLEADPAVLLRGELRHLADEDRGRLVEAFLVAVSHREVD
                     RWDPAVRSGEGALFHGGLADQLRAWIRDRTRDPRTRATACDIARQAGVSDLTEELADV
                     GLDPNEDMGVRVAAVSAVGDLGDVAARRRLVPLALDELGQQDPDDELKGAALLTTWPD
                     VLGPEEVLSALTPAKRDSLFGLYRHFVERAFPTTLPVDALPEALHWAASLPVEHQPTD
                     ALSDLREALVLRALDHTERHDVAEALVQVICALLNESVELLSYQAIKQRGGVLAEEST
                     RRRIVNRLVSAVADDSLSANAVVLATPPLLSENDVPFLIDKLMREYDVELQKGYVKLL
                     TAMLGRGVNPEPILEARGNSLALREATSAYFDAVPLHSELARQQRETHRRLKELRRKR
                     NERREPTLDVVARVEEALTAFERGDIDGFWHALYWLEIDGARRDRKVFVSDPRALPGW
                     QFLDDALRTRMVAAAEKYLQEGDPAPERWFGKRILHAPATAGYRALRLLAEVRDDRRE
                     LDTATWERWAPAVIGWPASGADDESAFNTWAVDRVIQRAAATSAEWIGRELDRDLRSG
                     DTPFVLHRVKAGWSSELEAAVLKRAKRSPLSAGARAEVLRFLIRHGSEAARQHAERLV
                     TPAALAAGGRRRDLALKVGALLAAESEDAAWSRLWPLIESDADFGKSLITELATGYER
                     QIAPSLTARQVGALYNWLLIHFPPEEDGHEEGAHFISPREEVGLWRDRVLASLTERAT
                     SAAVDELARLAAAHTNRPYLLARRAQAAQELCRRKWIAPSPAVIVQMTHNAGRRWVKS
                     AADLRRLLVNTIRDSEAQLQKRSQVYWLWNTAPLRPKPENALSDWLKSHLDDVLRERG
                     IVAGREVQIRPGPGHHMGEASDLLITAAAGERVEGAPLVEVVVEVKGCWHREVETALK
                     TQLCDRYLQAGGRDQGIYVVGWYDADGWDESDKASRSRCRRYTVAELSTMLERQAESV
                     SAGNGVSITAVVLDCSLPPPGSAGSARRG"
     gene            8485..10056
                     /locus_tag="VNU01_03375"
     CDS             8485..10056
                     /locus_tag="VNU01_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31692.1"
                     /translation="MDSAQQWPLQFTVLPNGGRLPSGARSSAYLLTDNWDDWFTYNTL
                     YVLYVYDDAGRQHRLGGVKIGQFGMTEGQRRPDLPSTFSTLDDRFFSLGQDAEYYEIL
                     QSLPASMRQHILTGLHDLAFDLILFDRALLEDVTGVSLLGSVARTTVLGQFHRLSTGG
                     VRLSRFDFGYEAPRASADHESSLRLTFKVRPESEPPTNTHVLIGRNGVGKTRLFDLMA
                     QALVNPGASREEVGAFFSADATGSPGSLFANVVSVTFSAFDPFQPLQRPQNRAVGPDY
                     AYIGLKRTRSLGESGPDGRGPKTPDDLAEEFGDSVRQCVSGPRAERWKRALAILESDP
                     IFRDAEVAGLAELSEKEAVLESRGRFMRLSSGHKIVLLTVTRLVETVEERTLVLLDEP
                     EAHLHPPLLSAFIRSLSDLLVNRNGVALIATHSPVVLQEVPRSCGWIMRRSGSYAVVE
                     RPEIETFGENVGILTRRVFGLEVSDSGFHTIIARAVAERLDLSEIASRFGHQLGGEAL
                     ALAQALIATRDSKDS"
     gene            complement(11995..12393)
                     /locus_tag="VNU01_03380"
     CDS             complement(11995..12393)
                     /locus_tag="VNU01_03380"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWH31693.1"
                     /translation="MSRARSRPAAGGAAREQRTAAARRALDALAPLGPVEARAMFGGH
                     GVYLEGAMLALVAGGEVYLKVDDVNRQLFVAAGLPPFVHVARGREITMSFHRAPEPLD
                     DWDSLEPFAESALAAARRALAARASGRRRG"
     gene            complement(12390..12734)
                     /locus_tag="VNU01_03385"
     CDS             complement(12390..12734)
                     /locus_tag="VNU01_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31694.1"
                     /translation="MTIPSDAIERERARRRQRNEQILNGLNERLEERIEEMRDTDLTA
                     GAPGDPIRYLCECSDLDCRGRVEMDPERFADVHRRPQDFVVLPGHEQPDIESVVARLP
                     EQALVVRKYDLG"
     gene            complement(12900..13634)
                     /locus_tag="VNU01_03390"
     CDS             complement(12900..13634)
                     /locus_tag="VNU01_03390"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31695.1"
                     /translation="MTAGIGDSLQRASDTLFEWLPRLIGALVILLVAYLIARAVAALL
                     ERVLGKAGIDRALHSGGVGARVKQAAPDLSPSSVIAKIAFWLIFLTGALIALSALGIP
                     AIDNFVAQAIAYLPNVVAAILIVAIAVAISGAVAAAARRFMGDTAMGRIVSVAAPAVI
                     LTIAGFMALVQLRIAVPIVTGAFYIVLGAIALGAAIAFGLGGQGAARRLIDEAYERGR
                     QNLPQAKAEAQIGKQRAEQEAHDRMG"
     gene            13783..14784
                     /locus_tag="VNU01_03395"
     CDS             13783..14784
                     /locus_tag="VNU01_03395"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="HWH31696.1"
                     /translation="MGTQIDLGQPGLLQGRTVYGSDGEKLGTVSDVLTDDSGRVEYVE
                     VKSGWFGTKRHVVPASSLTAGDGDDLLTPYSKDQLENAPTFDEDEHVDYDAEQRIGAH
                     YGTDVRDWDDSRDAWHRGEDLSRGPTPETRHPDGALDDAADTTQGPTPTTRTAMRSTD
                     DDAAASGQPSTDSRTREGAGTDMDMGRTGSGADAPPMGDDLSGPETDNAMTRSEEELR
                     VGTERREAGRVRLRKWVETEQQTRTVPVQHEEVRISREPVTDANMGRAMDGPSISEEE
                     HEITLTRDEPVASVEAHPVERVRLDKETVTEQQEVSGEVRKERIEVEGDEGLRGDRM"
     gene            14781..15308
                     /locus_tag="VNU01_03400"
     CDS             14781..15308
                     /locus_tag="VNU01_03400"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HWH31697.1"
                     /translation="MSVPPEAEGRSGGGDRPGEVPGRDAPIELVRTEEELVVGMRSEE
                     AATVRVRTEVETRHVRHLVPVTAEDVEVFEVEVPEGAEDSGQVETTPEGDVSIPVTEE
                     RLFVVRRPVVVKRVVVRRVARPAGRRPVEGELRAQRVVVEHADDPDRRWEARPTPGRE
                     RVRMEEPPAGGGDPA"
     gene            complement(15534..>15743)
                     /locus_tag="VNU01_03405"
     CDS             complement(15534..>15743)
                     /locus_tag="VNU01_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWH31698.1"
                     /translation="VAEPGTPAAGAGVRAGDVIVTVDGEPVDAMEDLYAALRARAPGD
                     RLPLEIARGGGRLSVTPVLADRPEG"
BASE COUNT         2446 a         5454 c         5451 g         2392 t
ORIGIN      
        1 gcccgcgatg gagccgagcc gggcggcgat gttcaccgtg tcgccgagcg ccgagaagtc
       61 gacgcggtcg tcggtcccca gcgcgcccac gaacatgtcc cccgtgtgca cgccgcagcc
      121 gacgcccagc gcggccaggt caccgtcggc gcggcgcagg gcggcgagga ggtcccgcgc
      181 ggtgcccacc gccgcacgcg cgtgcgcgtc gccggaggtg agcgggatgt agagggccat
      241 cacgccgtca ccgaggaact tgtccaggat gccccggtgc cgctcgatgg ccgtggcggc
      301 cagggcgtag aagcgctgca gcagccggtg gtactgcgcg gccgtgcgac cctccgcgag
      361 acccgtggag ccgcgcacgt cggcgaacag gacgctgccg ggcacctccg cgccgccggg
      421 cctgcggcgc aggctccaca tgcagacgcc gcacatcagc gggttctcgg gggagcgccc
      481 gtgcatcgcg atccgcgtca ccattcgcgc gggcccccgg aagggggcgg cgcagacctt
      541 gcaacggggc gtgcccggga gccggttgaa cacggtgcgg agccgcttga gcgtggagtc
      601 ctcgccgagc agcaggccgc gccacatctc cgtcagggcg gcctccgaga gcggttccgc
      661 cggccggtgc cgcgcccggc gggccggccg tcggcccggt cgcgtccctc cgccgtggcc
      721 gctgggcatc gtgagaccct ccatcgtcgc cccgttgccg agcctatggc ggtaccgggc
      781 gccggcacca gtgcctcagg cccggggcgc gcctcgggga gccgtgggcg cggcccggcc
      841 gggaaggccc ccggccggcg tgcgcaggtg cccgcgccac gccgcccggt ggcggccggc
      901 accgtcgcgc cagctccagc gcacctccac cgccgcgtcg agcacgccca ccggccagga
      961 aaacgaggcc cgcaggccgt gcgcggtggc cccgtccggg ctgacgccga aggcactcgc
     1021 cccggtgagg ccgccctcca ggggacgccc ctccaccgtg gcgcccacgc tgacgtcctg
     1081 cgcggcccag gcggccatgt tctcgatgcc cacctccagc cgcaggccgc ccgccgggcc
     1141 cggaacgatg cgcgtcgcac gcagcaccgg ctgcgcgccg agcagcgccc gccgcccctg
     1201 ccgcagcatg cgcgccagga gcacggccag cacggagccc accagcgcca gcgcgagcgc
     1261 gccacccgcg gcgagttccg tggcgtgcgc gtccagccag ccggccatcc ccggacggta
     1321 gcgtccgctc aggtcggcgc cgtttgcgcc gatggaacgg tcgcctataa tcggccgtcc
     1381 gcgggggatt agctcagctg ggagagcgtc gcgctggcag cgcgaaggtc agcggttcga
     1441 gcccgctatc ctccacctcc gccgaagccc tgctctttgc agggctttcg tcgttttcgg
     1501 gccggaaggc gagctgctcg ggtagcaccg gggtagcacc ggaacgcatc acgccggccc
     1561 tcagcgcaca gagccgggcc tcgggttcgg ccagcagcac gtgcgcgtag acgtccaggg
     1621 tcaccgaggc gcggctgtgg ccgacgacct gtccgatcaa gggcagcggc acgcccgcca
     1681 tcaccagcag gctgatgtag cggtggcgca ggtcgtgcgg ggtggcgtgc gcgatgcccg
     1741 cgacctgcga cgcgcggagc atcgcgcggc cgagcccggc cggcgtgagc gcggggtcga
     1801 cgaccggcgc gagcggtgcg cgtgcgtctg cgggcgtcat cgcctccagg agcgtcaggg
     1861 tctcaggcat gaggggcacc caccgcctgg ccgcgcgcgt cttggtcccg cgcacacgca
     1921 gacgcgcgcc gcgcacgtcg atgtccgccc acctcaggcc ctcgagctcg cccacgcgca
     1981 ggccggtctg ctcgagcagg tccatcaccg cgtgccactt ggtgggcatc gcgctgcgca
     2041 gggcgaggaa gtcggcctgc gaggggggct ccgggacctc acgcacggtg cgcggcacgc
     2101 gcaccgagcg gtgccgggcc gcgttcgggc ggtcgaggtc ggcgaagtcg agcacctgct
     2161 tgatgacgcc cgggtacacc gcgaccgtct tcggcgacag ctcactctgc gcgttcaccc
     2221 acgcctgcac gtgcccggga cggatctcgc tcacctccat gcgcccgagc gcccccagcc
     2281 ggcgcatggt ctggcggtag tgcgcgtgag ccgccgcgcc cacgtcgacg cgcgtgtgcg
     2341 cgaactcctc gcacacctgg gcgagcgtgc gtgcgcgcgt ctccgcctgt gccgcgacgt
     2401 gcgtgagtgc gcgcacgtcc ggcacgcgca tgtgcgcaag ctcccccgct atccagtcac
     2461 ggcgcgcacg cgcttcgcgc agcgtgccga acgcgccgcc gtgcactgcg cgcgcctcgc
     2521 ggccgccgac gcggtacttg acgtggtagc gcacccctgt gcgcgaggtg cgccgctgga
     2581 tccagacgct cggcacgaga aatccgaggc taggggcgcc tgcgcgcgcc gatcaacacg
     2641 ggcgcacgcg tcctgagtcg taggtgtgcg tcctcaggta cgcctcgagg tcgtcgcggc
     2701 gcacgtagac cttgcggctg cccggcaggc tcaccacggg aagctcgcgg aacaggcgct
     2761 cgacgtccac gcgcgtgagg cccagatgcc ggcgaacggc ggctcggtca agaagggcgg
     2821 gtgtcggcgg aatcggcgcc atcaccagag ggatggcggc acgggtcggc acgagggggt
     2881 gctacccagc cctgctacga cagggcgctc acacccagca cctgaaggac tcagttcggc
     2941 agctccggga tgggcgggtc gatatcgcgt ctgaaggcgc cacccctcgg gctctgcccc
     3001 tccggccggg gctgcgcgtc ccgtgggcct ctgaggttgg gtgggggccc acccgacggc
     3061 tcacccgcgg cttcggacgg ccggtcggaa aggtcgagca tccgttgatg caactgtgcg
     3121 ccttgcaagc cgcggcctcg taagacagag ccgtcgcacg cataggccat ctgcacgcct
     3181 tggccagcgc cacccgaatt cggcgacgga gaaggcgtgg gatcggggcc ccggtgtcgg
     3241 agcagcacga agtcttccgc gtctccgatg ctgggacggt cgtgtgcagg agtcccggca
     3301 gctctcgctc gtggccgacg cctctagagg tccccgtcct acaagccgcg gtcgagagtc
     3361 acccggcgcg cctgctgagt cacgcgcgtc ctcgtcgtcg cctgctgtct cgaccgaggt
     3421 cgcgctgcgc gaccctagtc gccgcttggc cgctgcgcgg cgcagtcagc cccggcgcgc
     3481 cgaaccggcg cttcccggcg gtggcaggga gcagtcgagc acgaccgcgg tgatcgagac
     3541 gccgttgccg gcagagacgc tctcggcctg gcgttcgagc atggtgctga gctcggcaac
     3601 ggtgtacctc cggcatcggc tgcggctcgc cttgtcgctt tcgtcccagc cgtcggcgtc
     3661 gtaccagccc actacgtaga taccctggtc gcgcccgccg gcttggaggt agcgatcgca
     3721 caactgagtc ttgagcgccg tttccacctc gcggtgccag catcccttca cctcgacgac
     3781 gacctcgacg agcggcgctc cttcgacccg ctcgcccgcc gccgcggtga tgagcaaatc
     3841 ggaggcctcg cccatgtggt gaccgggacc gggacgaatc tggacctcgc ggcccgcaac
     3901 gatgccgcgt tcccgtagaa catcgtcgag gtgcgactta agccagtctg agagcgcgtt
     3961 ctccggcttg ggccgcaggg gtgctgtgtt ccagagccag tagacttgcg accgcttctg
     4021 gagctgcgcc tccgaatcgc ggatagtatt caccagtagg cggcgcaggt cagccgccga
     4081 cttgacccac cggcgccctg cattgtgtgt catctgaacg atcactgccg gcgaaggagc
     4141 aatccacttg cggcggcaca actcctgagc cgcctgtgct ctccgcgcga gcaggtaggg
     4201 acggttggta tgggctgccg ccaaccgagc aagttcgtcg acagcagcgg acgtggcgcg
     4261 ctccgttagc gatgccagca cgcgatcacg ccacaagccc acctcttccc gcggactgat
     4321 gaagtgagca ccttcctcgt ggccgtcttc ctctggaggg aagtggatca gtagccagtt
     4381 gtacagcgcg ccgacttgac gcgcggtcaa actgggggcg atctggcgct cgtagccagt
     4441 cgcgagttcg gtaatgagcg acttgccgaa gtcggcgtcg ctctcgatca acggccacag
     4501 ccgcgaccag gcggcgtctt cagactccgc cgcaagcaag gcgccgacct ttaaggccaa
     4561 gtcccggcgg cggccgccag ccgcgagagc cgcgggcgtg acaagtcgct cggcgtgttg
     4621 gcgtgccgcc tccgagccat gtcggatgag gaaccgcagc acctccgcgc gggcgccagc
     4681 cgagagaggg ctacgcttgg cgcgcttaag gacggctgct tcgagctcag aggaccaacc
     4741 agccttaaca cggtgcagca caaacggcgt atcgccgctg cgcaaatccc ggtcgagttc
     4801 acgtccaatc cactcggcgc tcgtagctgc agcacgctga atgacccgat caaccgccca
     4861 cgtgttgaac gcgctttcgt cgtcggcccc agaagccggc caccctataa cggcgggcgc
     4921 ccagcgctcc cacgtcgccg tatccaattc ccgccgatca tcgcgcacct ctgcgagcag
     4981 ccggagtgcg cgatacccgg cggtcgctgg cgcgtggagg attctcttgc caaaccatcg
     5041 ctccggcgca gggtcgccct cctgcaggta cttctcagct gccgccacca tccgagtgcg
     5101 caacgcatcg tccagaaatt gccatccggg aagcgctcgc gggtcagaca cgaacacttt
     5161 gcggtcgcgg cgtgcgccgt caatctccag ccagtacagc gcgtgccaga acccgtcgat
     5221 gtcgcctctc tcaaaggccg tcagtgcctc ctcgacgcga gcgacgacgt ctaacgtcgg
     5281 ttcgcgccgc tcgttgcgct tgcgtcgaag ctccttaagg cggcgatggg tctcgcgctg
     5341 ttgacgcgca agctctgagt gaagcggcac cgcgtcgaag tacgcggacg tcgcttcgcg
     5401 cagcgcaagg ctgttgccgc gagcttccag aattggctcc ggattgaccc cacggccgag
     5461 catcgccgtg aggagcttga catacccctt ctgaagttcg acgtcgtact cgcgcattag
     5521 tttgtcgatc aggaacggta cgtcgttctc ggagagaagt ggtggtgtcg caaggacgac
     5581 tgcattggcc gatagggaat cgtcggccac agcggaaacg aggcggttaa cgatgcgacg
     5641 ccgtgtactc tcctcggcga gcacaccgcc ccgctgcttg atcgcctggt agctcaggag
     5701 ttctacggac tcgtttagga gcgcacaaat cacctggacg agggcctcgg cgacgtcatg
     5761 ccgctcggtg tgatccaagg cgcgtagaac gagggcctcg cgcagatcgg agagcgcgtc
     5821 ggtcggttga tgttcgacag gaaggctcgc cgcccagtgc agcgcttcgg ggagggcgtc
     5881 gacgggcaac gtcgttggaa aggcgcgctc tacaaagtgc cggtagagcc cgaaaagcga
     5941 gtcgcgcttc gcgggggtga gcgcggaaag aacttcctcg ggccccagaa catcaggcca
     6001 cgtggtaagg agcgctgcgc ccttgagctc gtcgtcaggg tcttgctggc cgagctcgtc
     6061 tagagccagc ggtacaaggc gtcggcgagc agcaacgtcg ccaagatcac cgacggcgct
     6121 caccgccgcg acgcgcacac ccatgtcctc attggggtcg agcccaacgt ccgcgagttc
     6181 ctccgtaagg tcgctcaccc cggcttggcg agcgatgtcg caggctgttg cccgagtgcg
     6241 gggatcccgc gtccggtcgc gtatccatgc tcgcagctgg tcggcgagac caccgtggaa
     6301 aagcgctcct tccccgctgc gaaccgccgg gtcccatcga tcgacttcgc ggtgcgaaac
     6361 cgcgacgagg aacgcttcca cgagccgtcc gcgatcctcg tcggcgaggt gccgcagctc
     6421 ccctcggagg aggaccgccg ggtcggcttc gagcagccgc tgaacgaaag gtggcgactg
     6481 cgaggcgagc catgcggcga tctcgctgag ctgcgggatg actcggtagc cgcgccctgt
     6541 ctcgctgaag agcaggtcgt cgatctgggc gtcggagagc gagctgtacg ccaggtagcg
     6601 ggcggcgagg tattccgcca ccgtgggatg cgcgaatccg acacactcga cgccacgagc
     6661 ggagaagaga ccggtccgca gtgtctcatg gacctcgacc ggccctactg cgaacgcatc
     6721 gtggatcgcg cgcaggggtt cgatctcctg gccaccagcg agatcattta gcgccacttc
     6781 atcggggccg gcggatcgtt cgtcaagcct tatggatgag cggccggaga gcatcgtcgc
     6841 cgcggcgatc ctcgtggcca ccgccaggcg ctcgcccacc gagagtcggc gactgtcctc
     6901 gcgagggcgc tggggattcg gctcctcgca ccagcgcagg caggcctctt cgtacagctc
     6961 ggcgcggttc tccttaaggg gtccgccgtc gcacctctgg tcgagcagca tcttgagcgt
     7021 catcggactc atggctaaag cctgcaagcg cctgtcgatc acctcggtga cgaacgagtc
     7081 tccgtccagc ccgcgccccc ttgccgcctc agcgatctcc cgtcgccgaa ggggcaagag
     7141 ctcgtagatc tcgaatccgt tgggccggaa ccggtccttc agaaagtcct ctagcgccag
     7201 ctgccgatcc gcgctgcggc aagtgatccg aaggcttaag cggtcgatcg gcgcttctgc
     7261 gagcccttca atcagaaggc tggccaccgt gtccacgcgc agcagcgcct cgtccaaccc
     7321 gtcgaggaat acgtgcagca cgccgctggt cgcgctcagc cacgattgcc aggccgtaga
     7381 cccgaagata tttcgctcta acttcgcttc ctgacgcgtt gacccaaggt tcacggtcag
     7441 taggtggtcg gtgccacttc gtgcgaggct ggcggtcttc tgggcgtcgg ctcggagagc
     7501 cgtcgacttt cccaggcccg gctctccgat cagcatcagg caggtcccgc ccgtcgactc
     7561 cggcagcttg accacgtccg gggcaaaggc ccctagcggc ccgtcgggat ccggaacaaa
     7621 cccgtcgtcg acgtagctga ggcttccggt ggggcggacc cagacgcgtt cgatttgggt
     7681 gacggtcatt cggtggccgg gcgcggagat acggttcctt ggattctgaa gtttttctcc
     7741 gagcactgct cgatctcagt gagccgcagc ccgccgcgcc tcctaggcgg gcgcgtaatc
     7801 gctccctggt ctcgggtcct gccccccagc cacgcgtctt gtatccgtca gccgacccgc
     7861 tcacgcgggt cgtcatcgtc gtgcccaagg ccgtgcgccg ggccgcagcg ctgagccccg
     7921 ctgtgctatt ggtcgcaagg tgtgcagtac gacgcagcgc ggctgcgccg cgcggcttct
     7981 ccgctgcatt caaggcggac tgcacgcctt agccttgggg gccaacgcga cctctcgcgt
     8041 gggcgcgcgc cgcactggtg gcacaacccg gtcgtccccc ttgccgccga gcgaagacga
     8101 gccgaggctc cgggtcccgg actgcgcccg ccgacgtacc cgagaaccag gatcggcaca
     8161 acgctgagtc aacgtctgcc cgaattcgcg tgggccacga gtcgccaccg ccacccgccc
     8221 actcccgacc accgataccg ccccgagcaa cgtcctccac gccgtgcttc ccgaacatcc
     8281 agcctgcgac gtgacttcag accctccctg cgcgcccgcc gcgaagttgg tcgagcagca
     8341 ctcgattcgc ttgcagccag gaagacacgc tcagcccccg cacctcccga ctgtgccgct
     8401 tccaacacgg aacaatcctc ggccgagcca ctggagtcga ttcgagcatt gccaacgtag
     8461 ccagcgcact ggatactggt gaagatggat tcagcgcagc aatggccact ccagtttacc
     8521 gtcctgccca acggtggtcg cctcccgtca ggggcaaggt cgtccgcgta tctactaacg
     8581 gacaactggg acgactggtt cacctacaac acgctctacg tcctatacgt ctatgacgac
     8641 gctggccggc aacaccgact tggtggcgtc aagattgggc aattcggcat gaccgaggga
     8701 cagcgccggc ccgatctccc atcgacattc tcgaccttgg atgaccgctt cttctccctg
     8761 ggccaggacg cggagtacta cgagattctt caatccttac cggctagtat gcgacagcat
     8821 attctgacgg ggcttcacga ccttgccttc gaccttatcc tcttcgatcg cgcgctactc
     8881 gaagatgtta cgggcgtttc gctcttgggg tccgttgccc gtacgaccgt attggggcag
     8941 tttcatcgcc taagcaccgg gggcgtgcgc ctgtcgcgat tcgatttcgg ctatgaggcg
     9001 cctagggcca gcgccgacca cgaatcgtca ctcaggctaa cgtttaaggt ccgacctgag
     9061 tccgagccgc cgacgaacac gcacgttctc atcggcagga atggcgttgg caagacacgc
     9121 ctcttcgatc taatggccca agccctagtg aacccggggg ctagccgcga agaggtgggc
     9181 gccttctttt ctgcggatgc caccggctct cctggctccc tgtttgccaa cgtcgtatca
     9241 gtaactttca gcgcgttcga tcccttccag ccactgcagc gaccccagaa cagagccgtc
     9301 ggccccgact atgcatacat aggtctaaaa agaacgcgat cgcttggtga gagcgggcct
     9361 gatgggcgag gaccaaagac ccctgatgat ctcgccgagg aatttggcga tagcgtccgg
     9421 caatgcgtct ccgggccgcg tgccgagcgc tggaagcgcg cactggccat ccttgaatct
     9481 gatccgattt tccgtgacgc cgaagtcgcg ggtctcgccg aactttctga gaaggaggct
     9541 gttctagaat cgcggggccg gttcatgcgc ttgagttctg ggcacaagat cgtgcttctc
     9601 acagttacac gattggttga aaccgttgag gaacgtaccc ttgtcctcct ggatgagcca
     9661 gaggcgcatc tgcatcctcc cctcctttcc gccttcatcc gctccctgtc ggatttgctc
     9721 gtcaaccgta acggcgtcgc gctcatcgcc acacactccc ctgtagttct tcaggaggtt
     9781 ccccgctcct gtgggtggat catgcggcgg agtggttcat atgctgttgt ggagcggcca
     9841 gagatcgaga ccttcgggga gaacgtcgga attctgaccc gaagagtgtt tggcttagag
     9901 gtgtcggact ccggtttcca cacaataatc gctcgggcgg tcgcagagcg gctcgacctg
     9961 agcgagatcg cctcccgatt cgggcaccag ctcgggggcg aagcgctggc gctcgcgcag
    10021 gctctcatcg ctacacgcga ctcgaaggac agttagtgcg ctgccttgag aggccgcggc
    10081 ggcgcgcgtt gaagtcctac atcgtctgct cgagtcgaat taaagacgca ggtctacgtg
    10141 ggcgcatgga cggcattagg cccgagattg cacgcgccgg ggatgctttc gaggccgcga
    10201 tgaagagggg cgaccccgcg tccgtagcaa ccagcgataa cgtaggtgaa gtcaccgggg
    10261 ctgagatgtc tgcgctttac gtacagagga tggcgaagat tggtgcgccg ggtcggcaaa
    10321 tctatgacga gctagttggc gcggcgccag cgagtcggtg tccactgtgc gcacagcgac
    10381 gcgtttcgac tctagaccat tatctcccga aggcggctta cccatcgttg gccgttgcgc
    10441 cagacaacct aatcccggcc tgcgccgact gcaacaaaag caagtcaggc cgcagtccta
    10501 gtaaccgcgc gatcgcgacg cttcatccct actatgatgc ggtggaggat gaggtgtggc
    10561 tagaggcagc ggtcatcggg gaagcgccgg ctgccttgcg gttttacgtg ggcgacgtgg
    10621 gtgcatggga taaacattta ctggcgaggg ttaggtgcca ttttgaggtc ttcgggctcg
    10681 ggccgttgta tgctgttgaa gctgccgacg agatggcgag cattcgtgac tcgcttcggc
    10741 ggttagacga tgcaggcgga gccgcaagcg tcagggcttg gctggaagac cggagacaaa
    10801 gctgtcgagc gaacgctcta aacagttggc gcaccgcaac gtataccgcc ctggcgggga
    10861 gcgactggta ctgccaaggt ggattcaacc tctgagcgat ctgggccgtg cgtgccggcg
    10921 tgagtcgcga tctcatttcc cgctgaagtg ggccggcacc ggcgatcgga ccgctgaggt
    10981 ggcggtgagt gttcagcgtg agcgcccgct gtttcggccg tacaccctcc tgtcagggag
    11041 gagtcggagg ggaggtcgtg gtctccggtc taccggtttc ggagggctgc cgtcggtgcc
    11101 ggatcctctg gtggggcctg agcggtgaac cggtgtgggc gaacgtgggc ctggagttgg
    11161 agtgagagtc ccgaagggaa caccgccccg cccggaccca tgcgacggct gatggtcgtc
    11221 gggcgcgtgc ctccgcagtt gacgctagga gaggcgcagc gtctcgatca atcctccgag
    11281 tctccgttat cgcgacaggc agcccgcctg caacagtcgg cgaagccgtg aacgcggcag
    11341 accagcgatc ggcgcgaagc tggacatccg atcccgcacc aagcttgtcg ttcgcgtcga
    11401 ggtggttacc ggttaggccc cgagctagcg ttcccaggtt cccgacagga cacgcggccc
    11461 ggcactcgca gcaggagcca gggcgaagca gctcccccgt gacgcgctgc cgcgacgacg
    11521 gctgccccac accgtggcta gccgcctccg ctcgttggcc tcagcctgag aggggctaag
    11581 aacgacgcac aaggaccagc agaagcgaca gcttctcgag aaggccagta agggctcggg
    11641 actggcgtca tcccagattc gtcaacaggt ccgacggcga ctcatcggct cttggtcggg
    11701 atccagccgg gggaacacat gcgcccgcgc tgcgacgcct gcccctttcc agcagcgttc
    11761 atgacggagc acaaggcgca gtgcggcgtc ggtgccttcg agcgtaacgg gtagcacccg
    11821 ggtagcacgg cggggcgccg gacgagcatg caacgctcaa aaaagcccta tttcaagggc
    11881 tttccgtcgc ccccttcccc ccctgaagag ccgagcccgc tatcctccac ctcgtcgacg
    11941 ccctgcccct gtgcagggcg tccgtggttc tcaggcggca cccgaccacc ggtgctagcc
    12001 gcgccgccgg cccgatgcgc gtgcggcaag ggcccgccgc gcggcggcca gggcggactc
    12061 ggcgaacggc tccagggagt cccagtcgtc cagcggctcg ggggcgcggt ggaagctcat
    12121 cgtgatctcg cggccgcggg cgacgtggac gaacggcggc agcccggcag cgacgaagag
    12181 ctgccggttg acgtcgtcca ccttcaggta gacctcgccg ccggcgacca gggccagcat
    12241 ggcgccctcc aggtagacgc cgtggccgcc gaacatcgcc cgggcctcga cggggccgag
    12301 gggcgcgagg gcgtcgaggg cgcgccgggc ggccgcggtg cgctgctccc gcgccgcgcc
    12361 gccggccgcc gggcggctgc gggcgcggct cacccgaggt cgtacttgcg cacgaccaac
    12421 gcctgctcgg gcagccgggc gacgaccgat tcgatgtccg gctgctcgtg cccggggagc
    12481 accacgaaat cctgcggacg ccgatggacg tcggcgaagc gctccgggtc catctcgacg
    12541 cggccgcggc agtcgaggtc ggagcactcg cacaggtagc ggatcgggtc gcccggcgca
    12601 ccggcggtga ggtccgtgtc gcgcatctcc tcgatccgct cctcgagccg ctcgttcagc
    12661 ccgttgagga tctgctcgtt gcgctggcgc cggcgcgctc tctcgcgctc gatggcgtcg
    12721 ctggggatcg tcatggcccc cggttacccg ttggcggcct ccgccatcgg cgtcgcgtgg
    12781 gtcccatcgc cgacccccgt gcgccagccc cggggggccg gcgcgagggc ctccgccccg
    12841 gccgcggctc agcggacgac cggccgcggc cggggcggag ttcggggggc gccgggcggc
    12901 taccccatcc tgtcgtgggc ctcctgctcg gcccgctgct tgccgatctg ggcctcggcc
    12961 ttcgcctgcg gcaggttctg ccggccgcgc tcgtaggcct cgtcgatcag ccggcgcgcg
    13021 gcgccctgcc cgccgagccc gaaggcgatg gcggcgccga gcgcgatggc gccgagcacg
    13081 atgtagaacg cgccggtcac gatcggcacg gcgatccgca gctgcacgag cgccatgaag
    13141 cccgcgatcg tcaggatcac cgcgggggcg gcaacggaca cgatgcggcc catggcggtg
    13201 tcgcccatga agcggcgggc ggctgcggcc acggcgccgg agatggcgac ggcgatcgcc
    13261 acgatcagga tggccgcgac gacgttcggc aggtaggcga tcgcctgcgc gacgaagttg
    13321 tcgatggccg ggatgccgag cgccgacagc gcgatgaggg cgccggtcag gaagatgagc
    13381 cagaaggcga tcttcgcgat gacgctgctc gggctgagat cgggcgccgc ctgcttcaca
    13441 cgagcgccca cgccgccgga gtgcagcgcg cgatcgatac ccgccttgcc cagcacgcgc
    13501 tccagcagcg cggccaccgc gcgggcaatc aggtaggcga ccagcaggat caccagggcg
    13561 ccgatcaggc gcgggagcca ttcgaacagc gtgtcggacg cccgctgcag gctgtcgccg
    13621 attccggcgg tcatgaagtg cacgtcgttc tccttcttcg ggtccggggt ccgctcgggc
    13681 tgccccgtcc gtctccaccg caaccctcga cccggctgcg cgcttgacgc tcgggggcag
    13741 gggtacgcgc cgacccgtca cccacttccg aggaggccag acatggggac ccagatcgac
    13801 ctcggtcagc cgggcttgct gcagggccgc accgtgtacg gcagcgacgg cgagaagctc
    13861 ggcacggtca gcgacgtcct gacggacgac tccgggcgcg tggagtacgt ggaggtcaag
    13921 agcggctggt tcggcaccaa gcggcacgtc gtgccggcct cgtcgctcac cgccggcgac
    13981 ggcgacgacc tgctgacgcc gtacagcaag gaccagctcg agaacgcgcc gaccttcgac
    14041 gaggacgagc acgtggacta cgacgccgag cagcgcatcg gcgcccacta cggcaccgac
    14101 gtgcgcgact gggacgactc gcgcgacgcc tggcaccggg gcgaggacct ctcccgcggg
    14161 ccgacgcccg agacgcggca ccccgacggc gccctggacg acgccgccga caccacccag
    14221 ggcccgacgc ccacgacccg gacggcgatg cggtcgaccg acgacgacgc ggccgcgtcg
    14281 gggcagccgt cgaccgactc gcgcacccgc gagggcgccg ggacggacat ggacatgggc
    14341 cggaccggct cgggtgccga cgcgccgccc atgggcgacg acctgagcgg cccggagacc
    14401 gacaacgcga tgacccgctc cgaggaggag ctgcgcgtgg gcaccgagcg ccgcgaggcc
    14461 ggccgcgtgc ggctgcgcaa gtgggtggag accgagcagc agacccgcac ggtgcccgtt
    14521 cagcacgagg aggtgcggat ctcgcgcgag ccggtgacgg acgccaacat ggggcgcgcc
    14581 atggacgggc cgtcgatctc cgaggaggag cacgagatca cgctgacgcg cgacgagccc
    14641 gtggcgtcgg tcgaggccca cccggtcgag cgcgtgcggc tcgacaagga gaccgtcacc
    14701 gagcagcagg aggtctccgg cgaggtgcgc aaggagcgca tcgaggtgga gggcgacgag
    14761 ggcctgcgcg gggaccggat gtgagcgtgc cgccggaggc cgaggggcgc tccggcggcg
    14821 gcgaccggcc gggcgaggtg cccggccggg acgcgccgat cgagctcgtc cgcaccgagg
    14881 aggagctggt cgtcgggatg cgttccgagg aggcggcgac cgtgcgcgtg cgcacggagg
    14941 tggagacccg gcacgtccgg catctcgtcc ccgtcaccgc cgaggacgtc gaggtgttcg
    15001 aggtcgaggt gcccgagggt gccgaggact cggggcaggt ggagacgacg cccgagggcg
    15061 acgtgtccat ccccgtcacc gaggagcgcc tgttcgtcgt gcggcggccc gtggtggtca
    15121 agcgcgtcgt ggtgcggcgg gtggcccgcc cggccggccg acggccggtg gagggcgagc
    15181 tccgcgcaca gcgggtggtc gtggagcacg ccgacgaccc ggaccgccgc tgggaggcgc
    15241 gccccacgcc agggcgcgag cgtgtgcgga tggaggagcc gccggccggc gggggcgacc
    15301 ccgcctgacg ccggacgccg ccgcgccgga acgcggcgcg gcgggccacc gcggggcccg
    15361 ggtccggagc gtcgccccgc gcgccgacat cgccgcggtt catctcgcgg tgccgccccg
    15421 gtgcggggtg ggtcgtccga atccgcccgg tccggcgtcg gccctcgcac gcggcggtgc
    15481 gatgacccgg gcgtgccggg tcccgtgaga ggcggccgca cgggcccggc ctgtcagccc
    15541 tccggccggt ccgccagcac gggcgtcacg gagaggcggc ccccgccgcg ggcgatctcc
    15601 agcggcagcc ggtcgcccgg cgcgcgtgcg cgcagcgccg cgtagaggtc ctccatcgcg
    15661 tccaccggct ccccgtcgac ggtgacgatg acgtcccccg cccgcacccc ggcgcccgcg
    15721 gcgggggtgc cgggctcggc cac
//
LOCUS       DATMKC010000115         6482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_5294, whole genome shotgun sequence.
ACCESSION   DATMKC010000115 DATMKC010000000
VERSION     DATMKC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5294"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VNU01_03410"
     CDS             <1..614
                     /locus_tag="VNU01_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31699.1"
                     /translation="TAFLVPQDSPGYEGRQIHGKLGLRAGDTAEISLRDVRVPDRYRL
                     GELGEGMRIALTALDSGRYSLAAGCTGICQASLEHSLRYASERTQFGKPIARFQLVQQ
                     LLAEIHMDTEAARALYWKVAWKHGLGERHTLESSVAKTFCSEAAVRCADKAVQVHGGY
                     GYIDEYPVAKLLRDARVTTLYEGTSQVQRLLMGRALTGENAFT"
     gene            617..1453
                     /locus_tag="VNU01_03415"
     CDS             617..1453
                     /locus_tag="VNU01_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWH31700.1"
                     /translation="MYDLHTHTVFSDGTTTPEDNAALAAAAGLDGVALTDHDTLDGWE
                     RMAAACEAAGVEFVPGVELSAEADGRSVHVLGYWVDPADPALRAECVRLRAERERRGR
                     VMVERLAAHGTGVTWDEVAVIAGPAPVGRPHLASALVARGAARDIEHAFTRWLAEDGP
                     VHEPKRALAPAAAVRLVVEAGGAAVLAHPASTFPGPALPDALLDELRACGLRGLEADH
                     PAHDEASTRRWRDAAASRGLLVTGASDFHGGRKAVRIGTRTTPDGVVALLREASRGGA
                     RW"
     gene            1447..2040
                     /locus_tag="VNU01_03420"
     CDS             1447..2040
                     /locus_tag="VNU01_03420"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWH31701.1"
                     /translation="MVGPAKRLTPLPMFVVTSDGALKGRVLVVDDDELVLGRRDNSDL
                     VLPDPHVSRAHAVVRRSSGAIWLEDLGSTGGTFVNGEQITGSQALKHGDLVRFGTVET
                     RFEDRSSLVDQDDATQMMALPEVEERPVLSPRQQEVLGYLQLGLTNPEIAVKLGVTER
                     TVKAHCQEVFDRLGARNRTAAVAAAMRLRLLPEDPEA"
     gene            complement(2047..3018)
                     /locus_tag="VNU01_03425"
     CDS             complement(2047..3018)
                     /locus_tag="VNU01_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWH31702.1"
                     /translation="MSVLRVALVGAGSMGTLHARVIAESTGATLVQVVDPDPIRGGAL
                     AAAHGAVWTPEGPLDDCDAVVVAAPTEHHLAWAQQAIAARLPVLVEKPLSAHPLEAAA
                     MIDAADAADVPLMCGFLERFNPAVRTALSVVPEPVHLHAVRHSPYHQRIKVGVSHDML
                     IHDVDLVVRLAGMPSTVAAGLIVHHEKSDADAEDAVEAVLGYPSMLAALSASRLSQTK
                     VRRLSLAGQEQLAEVDLVMGTVTVYRHVLAEFVAGGDRTYRQQTVVDVPFIRKPAEPL
                     AAQFAHFLALARGEADAAAERATLLAPHMLVARILDSARRPEPAPVV"
     gene            complement(3015..4142)
                     /locus_tag="VNU01_03430"
     CDS             complement(3015..4142)
                     /locus_tag="VNU01_03430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWH31703.1"
                     /translation="MSSERVPISKVPVNEDIESLVLEVLRSGHLAQGPMVARLEAAFA
                     ELCAVPHAIAVSNGTVALEAAAAALGLGPGDEIITTPFTFAATANAMLHTGATVRLAD
                     IEPDTFTLDPEAVESLVGPRSRAIVPVHLYGHCADMTALAALAERDGLALIEDAAQAH
                     GATWKGAPAGGFGVGCFSLYATKNVTSGEGGVVTTRDDDVAARLRLLRNQGMSRRYQY
                     DLPGHNWRLTDVQAAIALPQMDRLAERTAARRRNAAVLSAGLADLPGLITPTTHAAAE
                     HVFHQYTVRVTPDAPLGRDALAAALDAAGVDTGAYYPKLVGDYDCYASHPNLVVDPTP
                     RAARAAREVLSLPVHPELTHEQLERVVTAVRDAFTKAGGTS"
     gene            complement(4139..4852)
                     /locus_tag="VNU01_03435"
     CDS             complement(4139..4852)
                     /locus_tag="VNU01_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31704.1"
                     /translation="MTRVHPSAVIGPGVQLAPGVIVGPHAVLLGPCEVREGAWIGAGA
                     IVGAPPGDVAAPHRSWDEPQGGGVVVGERARVREHAQIESGQAASTIVGPDAFVMPHA
                     HLSHDCVLGTHVVIASGAVLGGTVRVGDHVFVGLNTSVHQRVVIGAHAVVGLQSAVVD
                     DIPPFARAAGSPARILGVNHVGMRRRGLDRLAPAAEEILLRGGAPGDASVADELADRF
                     SEFAAWRRGSDRRTAKVAR"
     gene            5277..5921
                     /locus_tag="VNU01_03440"
     CDS             5277..5921
                     /locus_tag="VNU01_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWH31705.1"
                     /translation="MPSPADVFHAATFLTTFVTVLVIMDPVGNVPVFLSLSAAMEGPA
                     RRRAARQAVTVAGAVILTFALFGQQILHLLSISLEALQVAGGLLLVLIALELLRPEDD
                     ASIAEVGRNVALVPLGTPLLAGPGAIAATMLAMRRADTVAEVSSVVLAIVAVLVVAWA
                     ALRFSLVISRVLKENGIHLISRVMGVLLAAIAVQLVASGVAGWVEHGVQGATAG"
     gene            complement(5925..>6482)
                     /locus_tag="VNU01_03445"
     CDS             complement(5925..>6482)
                     /locus_tag="VNU01_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31706.1"
                     /translation="AAVLHRVGPPPVVDSGNLGWLTRTTARHYAAHRAAAPVLATALV
                     LSEGVPAETRRTVQEALLLADAVVVLERQDMEATELAVLGISPGAVQVRALDWQDDEG
                     LDEILASLRCEQVLVLGAGETLDLTSYEEARVEIESLPDGPTQVRVGPDASPEVRLHP
                     PTLEALAHIGSQDGPVAQTFAILPV"
BASE COUNT          854 a         2313 c         2426 g          889 t
ORIGIN      
        1 ccaccgcgtt cctggtgccg caggactcgc ccggctacga gggcaggcag atccacggca
       61 agctcggcct gcgcgccggc gacaccgccg agatcagcct gcgcgacgtg cgcgtgccgg
      121 accgctaccg cctgggcgag ctgggcgagg gcatgaggat cgcgctcacc gcgctggact
      181 ccgggcgcta ctcgctggcc gccggctgca ccgggatctg ccaggcgtcg ctggagcact
      241 ccctgcggta cgcctccgag cgcacgcagt tcggcaagcc gatcgcgcgg ttccagctcg
      301 tgcagcagtt gctcgccgag atccacatgg acaccgaggc ggcgcgcgcc ctgtactgga
      361 aggtcgcgtg gaagcacggc ctgggcgagc gccacacgct ggagtccagc gtcgccaaga
      421 cgttctgctc ggaggcggcg gtgcgctgcg ccgacaaggc cgtgcaggtc cacggcggct
      481 acggctacat cgacgagtac ccggtggcca agctgttgcg cgacgcgcgc gtgacgacgc
      541 tctacgaggg caccagccag gtgcagcgcc tgctcatggg ccgcgcgctc acgggcgaga
      601 acgcgttcac ctagtcgtgt acgacctgca cacccatacg gtcttctcgg acggcaccac
      661 cacgccggag gacaacgccg ccctggcggc ggcggcgggc ctggacggcg tggcgctgac
      721 cgaccacgac acgctggacg gctgggagcg catggcggcc gcgtgcgagg ccgcgggcgt
      781 ggagttcgtg ccgggcgtgg agctgtcggc cgaggcggac ggccgcagcg tccacgtcct
      841 gggctactgg gtcgacccgg ccgatccggc cctgcgcgcc gagtgcgtgc ggctgcgcgc
      901 ggagcgggag cgccgtgggc gcgtcatggt cgagcgcctg gcggcccacg gcacgggcgt
      961 gacctgggac gaggtcgccg tcatcgccgg cccggcgccc gtcgggcgtc cccatctggc
     1021 ctcggcgctg gtcgcccgcg gcgccgcgcg agacatcgag cacgcgttca cgaggtggct
     1081 ggccgaggac ggcccggtgc acgagccgaa gcgcgccctg gcgcccgcgg ccgccgtgcg
     1141 gctcgtcgtg gaggccggcg gtgcggccgt gctggcccat ccggcgagta cgttccccgg
     1201 accggccctg ccggatgcgc tgctggacga gctgcgagcc tgcgggctgc gcgggttgga
     1261 ggcggaccat ccggcgcatg atgaggcgtc gacgaggcgg tggcgggacg ccgccgcctc
     1321 gcgcggcctg ctcgtcaccg gtgccagtga ctttcatggc ggccgcaaag cggtcaggat
     1381 tgggacacgc acgacccctg acggggtggt ggccctgttg cgcgaggcaa gccgaggagg
     1441 cgcgcggtgg taggtccggc gaagcggctg acgccgttgc ccatgttcgt cgtcaccagc
     1501 gacggcgcct tgaaggggcg cgtgctggtg gtcgacgacg acgagctggt cctgggccgg
     1561 cgcgacaact ccgacctggt gctgcccgac ccgcacgtca gccgcgccca cgcggtggtg
     1621 cgccgctcgt cgggcgcgat ctggctggag gacctgggct ccaccggcgg cacgttcgtc
     1681 aacggcgagc agatcaccgg ctcccaggcg ctcaagcacg gcgacctggt gcgcttcggc
     1741 acggtggaga cgcgcttcga ggaccgctcg tcgctggtgg accaggacga cgcgacgcag
     1801 atgatggcgc tgcccgaggt ggaggagcgc ccggtgctgt ccccgcgcca gcaggaggtg
     1861 ctgggctacc tgcagctcgg gctgaccaac cccgagatcg cggtgaagct gggcgtcacg
     1921 gagcgcaccg tgaaggcgca ctgccaggag gtcttcgacc ggctgggcgc gcgcaaccgt
     1981 acggccgcgg tggccgccgc gatgcgcctg cgcctgctgc cggaggaccc ggaggcctga
     2041 tccggatcac accaccggtg cgggctccgg gcgccgcgcg ctgtccagga tgcgcgccac
     2101 cagcatgtgc ggcgcgagca gggtcgcccg ctcggcggcg gcgtccgcct cccctcgcgc
     2161 cagcgccagg aagtgcgcga actgggccgc cagcggctcg gcgggtttgc ggatgaacgg
     2221 cacgtccacc acggtctgct gacggtaggt gcggtcgcct ccggccacga actcggccag
     2281 cacatgccgg tagaccgtga cggtccccat gacgaggtcc acctcggcca gctgctcctg
     2341 gccggccagg gacagtcgcc gcactttggt ctgggacagg cggctcgcgg acagcgcagc
     2401 gagcatgctg ggatagccga gcaccgcctc gaccgcgtcc tcggcgtcgg cgtcggactt
     2461 ctcgtggtgg acgatcaggc cggcagcgac agtggacggc atgccggcca gccgcaccac
     2521 gaggtccacg tcgtggatga gcatgtcgtg ggaaacgccg accttgatgc gctggtggta
     2581 gggggagtgg cgcaccgcgt gcaggtgcac cggctccggt acgacgctca acgcggtgcg
     2641 cacggccgga ttgaagcgct ccaggaagcc gcacatgagc ggcacgtcgg cggcgtccgc
     2701 ggcgtcgatc atcgccgcgg cctccagcgg gtgcgccgac agcggcttct cgaccagcac
     2761 cggcagccgc gcggcgatcg cctgctgcgc ccacgcgagg tggtgctccg tgggggccgc
     2821 caccaccacg gcgtcgcagt cgtccagcgg gccctccggc gtccacacgg cgccgtgtgc
     2881 ggcggccagc gcgccaccgc ggatgggatc cgggtccacg acctgcacga gtgtcgcgcc
     2941 cgtggactcg gcgatcaccc gtgcgtgcag cgtgcccatg ctgcctgcgc cgaccagcgc
     3001 gacgcgcaga acgctcatga tgtgccacct gccttcgtga acgcgtcgcg cacggcggtc
     3061 acgacacgct ccagttgctc gtgggtcagt tccgggtgga cggggagcga caggacttct
     3121 cgggcggccc gcgccgcgcg cggcgtgggg tccaccacca ggttcgggtg ggaggcgtag
     3181 cagtcgtagt cgcccaccag cttcgggtag taggcgccgg tgtcgacgcc ggccgcgtcc
     3241 agcgcggctg cgagggcgtc gcggcccagc ggggcatccg gggtgacgcg gacggtgtac
     3301 tggtggaaga cgtgctcggc ggcggcgtgc gtggtcgggg tgatcaggcc tggcaggtcc
     3361 gcaaggcccg cgctcagcac cgcggcgttg cgcctgcgtg cggcggtgcg ctcggccaac
     3421 cggtccatct gcggcagtgc aatcgccgcc tgcacgtcgg tcagccgcca gttgtgcccc
     3481 ggaaggtcgt actggtaacg acggctcatc ccctggttgc ggagcagccg cagcctggcc
     3541 gccacgtcgt cgtcgcgagt ggtgaccacg ccgccctcgc cggacgtcac gttcttcgtg
     3601 gcgtacaggg agaagcagcc gacgccgaag ccgccggccg gcgcgccctt ccacgtcgcg
     3661 ccgtgcgcct gcgcggcgtc ctcgatcagc gccaggccgt cgcgctcggc cagcgccgcc
     3721 aaggccgtca tgtccgcgca atggccgtac aggtgcacgg ggacgatcgc ccggctgcgc
     3781 ggcccgacca gggactcgac cgcctcgggg tccaacgtga acgtgtccgg ctcgatgtcc
     3841 gcgaggcgca cggtcgcgcc tgtgtgcagc atcgcgttgg cggtcgccgc gaaggtgaac
     3901 ggcgtggtga tgatctcgtc gccgggaccg agtcccaggg ccgcggccgc cgcctccagc
     3961 gccaccgtgc cgttgctgac cgcgatcgcg tggggcaccg cgcacaactc tgcgaaggca
     4021 gcctccaggc gcgcgaccat ggggccctgg gcgaggtgtc cggagcgcag cacttccagg
     4081 acgagggact cgatgtcctc gttgacgggc accttcgaaa tggggacgcg ttcgctgctc
     4141 accgtgccac cttggcggtc ctgcggtcgg atccgcggcg ccaggccgcg aactccgaga
     4201 acctgtcggc gagttcgtcc gccacagaag catcgccggg ggcgccgccg cgcagcagga
     4261 tctcctcggc cgccggggcg agcctgtcca gtccgcgccg gcgcatgccg acgtggttca
     4321 cgcccaggat gcgcgcgggc gacccggcgg cgcgcgcgaa cggggggatg tcgtccacga
     4381 ccgccgactg caggcccacc acggcgtgcg cgccgatcac gacgcgctgg tgcacgctcg
     4441 tgttcaggcc cacgaagaca tggtcgccca cacgcacggt gccgcccagc acggcccccg
     4501 aggcgatcac cacgtgcgtc ccgagcacgc agtcgtggct gaggtgcgcg tgcggcatca
     4561 cgaaggcgtc cggaccgacg atcgtggagg ccgcctggcc gctctcgatc tgggcgtgct
     4621 cgcggacgcg cgcgcgctcg ccgaccacga cgccgccgcc ctgcggttcg tcccatgacc
     4681 gatgcggcgc cgcgacgtca cccggaggcg caccgacgat cgcgccggcc ccgatccacg
     4741 cgccctcacg cacctcgcag ggaccgagca gcacggcgtg cggaccgacg atcacgccgg
     4801 gtgccaactg gacaccggga ccgatcacgg cgctggggtg cacacgcgtc acgctgtcct
     4861 cctccgacgc ccgccggcgc cgccgggacg agcataacga gcaccgcggc cgttgcgggc
     4921 ggacgacgcc ggcccggacc cggccgccgg cttgctcagg ttggcaggtg tgtcggccga
     4981 tgcctgacgg tagccttgtc ggtccagcgg gccggccagg ccccgtcctc ctgttacaac
     5041 cgctcggagg ctccacacca acaccagtgg gctcaactca ggcggcccga cccgcgaggg
     5101 caggaccctc actcgggaag cgtcgccggg agccccatac tggcagcccc tccccggggg
     5161 cctcggccca ccgggatgac gctccagagt ggaacattcg ctctgagacg ggcttgtgtc
     5221 ggagggcggg acctggccgg cctgccgggt ccgcggtcgg ggggtagggt gcgcgcatgc
     5281 cctccccggc cgacgtcttc cacgccgcga cgttcctgac caccttcgtc accgtgctgg
     5341 tgatcatgga tccggtcggc aacgtgccgg tgttcctgtc gctgagcgcg gccatggaag
     5401 gaccggcacg ccggcgcgcc gcgcgccagg cggtgacggt ggcgggcgcg gtgatcctga
     5461 ccttcgcgtt gttcggccag cagatcctcc acctgctcag catctcgctc gaggcgctgc
     5521 aggtcgccgg cgggctgctg ctggtgctca tcgccttgga gctgctgcgt ccggaggacg
     5581 atgcctccat cgctgaggtg ggccgcaacg tcgcgctcgt cccgctgggc accccgctgc
     5641 tcgcggggcc gggcgcaatc gcggcgacga tgctggcgat gcggcgggcg gacacggtcg
     5701 cggaggtctc ctccgtggtc ctggcgatcg tggcggtgct ggtcgtggcc tgggccgccc
     5761 tgaggttttc gctggtgatc agccgtgtgc tcaaggagaa cggcatccac ctcatctcgc
     5821 gcgtcatggg tgtcctgctc gcggccatcg cggtgcagct ggtggcgagc ggtgtcgccg
     5881 gctgggtcga gcacggcgtg cagggcgcca cggcgggctg agcgctacac gggcaggatg
     5941 gcaaacgtct gcgccaccgg tccgtcctgc gagccgatgt gcgccagggc ttcgagggtg
     6001 ggggggtgca ggcgcacctc gggggaggcg tccggtccga cgcgaacctg cgtgggcccg
     6061 tcgggcaggg actcgatctc gacccgcgcc tcctcgtacg acgtcagatc cagcgtttct
     6121 cccgcaccca gcaccagcac ctgctcgcag cgcaacgagg ccaggatctc gtcgagcccc
     6181 tcgtcgtcct gccagtcgag tgcgcggacc tgcacggccc ccgggctgat gccgaggacc
     6241 gcgagctccg tggcttccat gtcctgccgc tcgagcacga cgacggcgtc ggcgagcagg
     6301 agggcttcct gcaccgttcg gcgggtctcc gccggcactc cctcggagag gacgagcgcg
     6361 gtggccagga cgggcgcggc tgcacgatgg gcggcgtagt ggcgcgctgt cgtgcgggtc
     6421 aaccatccca ggttgccgct gtcgaccacc ggcggcgggc cgacccggtg caggacggcg
     6481 gc
//
LOCUS       DATMKC010000116         4467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_300265, whole genome shotgun sequence.
ACCESSION   DATMKC010000116 DATMKC010000000
VERSION     DATMKC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300265"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..816)
                     /locus_tag="VNU01_03450"
     CDS             complement(64..816)
                     /locus_tag="VNU01_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31707.1"
                     /translation="MDRGDAGGDDGLDALRRFRRTAAGFLVFLALAFASLVVAHGVDE
                     ARFSACLGMRDGPAGASIRPDLHVLPPSVDCHLTWQDGREQVRRVALDPIVPAAVAGV
                     VAAWAALAGAAVYAATRGGGAAMLDRAAATLGSGVLVGAVAVALVASIAHQDGAFAPV
                     PRRGIWTPLQAGWVAPAVLVSVASASFTAAVRARSRPWIAGVAAAVVLWLFLVVGAFV
                     HPATPRIGAALTAGVAGSVLGRVARAVRGRPA"
     gene            complement(826..1080)
                     /locus_tag="VNU01_03455"
     CDS             complement(826..1080)
                     /locus_tag="VNU01_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWH31708.1"
                     /translation="MPIYEYRCETCGADDERLQALGADAPVDGCQACGGPLKRRFSRV
                     AVKYQGWGFSSTDALVADNRGKDFRALRDRAERISDAGGG"
     gene            complement(1237..1464)
                     /locus_tag="VNU01_03460"
     CDS             complement(1237..1464)
                     /locus_tag="VNU01_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HWH31709.1"
                     /translation="MPKSRSKRSTYVPPKPPKPKPSPRWVPWLGLGLIGVGLVEILLN
                     YFVPGFPGGNLNLIVGFILMAGGLVTLSQWR"
     gene            1550..2347
                     /locus_tag="VNU01_03465"
     CDS             1550..2347
                     /locus_tag="VNU01_03465"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HWH31710.1"
                     /translation="MRGDSERLSESPEAAAGPAGQPAAPTPLTRAAFALTLVLLVLVL
                     VTAAHSRPATQDARLGRRVELVELIRAEQARVNGLAARVEELSGQIAAFEAQASADPE
                     LLAALQAEIAAMSGPAGLTPVQGPGLVVTLTDSSLEESSTGDLNDLVIHEQDLQAVIN
                     ALWAGGAEAMSVNGQRVLATTAIRCVGNTLLLHGSVYSPPYQIRSVGDPDAMRAELAR
                     DLAVRQFQRAAEQFQLGFSVVEEEAMLVPAFEGSAGLRSARALGART"
     gene            2344..3000
                     /locus_tag="VNU01_03470"
     CDS             2344..3000
                     /locus_tag="VNU01_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWH31711.1"
                     /translation="MSAPEAMPPEARRAAMRLVGWALVLGGLGVLSWLAWMVLATNAT
                     TKAAQEDLLEEWLRRGEAETPVIDGAAAQPAAGAGGPVAVMEFLRDGRRILHDEPVVV
                     VAGVTAADLTKGPGHYPGSALPGGPGNMAIAGHRTTYGAPFADIDRLAEGDEIRVEDG
                     DGVTWTYRVVEQRIVEPSDTTVIASDPLGNGRPTLTLTTCHPRYSNRQRLVVFAQLAP
                     "
     gene            3059..>4467
                     /locus_tag="VNU01_03475"
     CDS             3059..>4467
                     /locus_tag="VNU01_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH31712.1"
                     /translation="MSAMSNKPAVKKATAKKATVKKATVKKATAKKATAKKATAKKAT
                     AKRRMPDPAEIPRPWLASYPPGVPESYDYPLVPLTRFLDDAAQDFPHTEAVEFLGYAL
                     TYRELRDQVDRLATALHNLGVRKGDRVGIVLPNCPQHVVAVFAILRLGAVVAENNPLY
                     TEAELEHQINDAGCSVLIVLDPVYQRIAKLKGKLPSVRHVISTGIQDALPFPKNVLFP
                     LKGKKDGTYYAIPEGEGVLRLSELVARTAPAVQQAEVDPREDLAMLVYTGGTTGVSKG
                     VMLSHFNLVANAFQARLWMPDVQAGRENILCVLPFFHSYGLTTCLTLGILSAATLTLL
                     PRFDRDMVLKTIDKRKPTLFPGVPTIYVALNTAPDVRKYDLTSVRACLSGAAPLPVEV
                     ARQFEELTGAKLREGYGLTETSPITHANPIYGKAKKGSIGLPVTDTVCAIVDLDDPTK
                     PVEPGQPGSTGLVGSSRST"
BASE COUNT          682 a         1687 c         1540 g          558 t
ORIGIN      
        1 gatcgcgccg cgccactgcg gcgagaagat ggtcgagcgc cgcgaagtcg cccgcgagcg
       61 caactaggcg ggccggcccc gcacggcgcg cgccacgcgg cccagcacgc tgccggccac
      121 cccggccgtc agcgccgcac cgatccgggg cgtcgcggga tgcacgaagg cgcccaccac
      181 caggaacagc cacagcacca cggccgccgc cacgccggcg atccagggcc ggctgcgtgc
      241 ccgcacggcg gcggtgaacg acgccgacgc caccgagacc aggaccgccg gagccaccca
      301 cccggcctgc agcggcgtcc agatgccgcg ccggggcacc ggcgcgaacg cgccgtcctg
      361 gtgcgcgatc gaggcgacca gcgccaccgc gacggctccg acgagcacgc cgctccccag
      421 cgtggcggcg gcccggtcca gcatcgccgc gccgccgccc cgtgtcgccg cgtacacggc
      481 ggcgccggcc agcgcggccc acgcggccac gacgccggcc accgcggcag gcacgatcgg
      541 gtccagcgcc acccgccgca cctgctcgcg accgtcctgc cacgtcagat ggcagtccac
      601 cgacggcggc agcacgtgca ggtccggacg gatcgacgcg ccggcggggc cgtcgcgcat
      661 gcccaggcag gcgctgaagc gcgcctcgtc gacgccgtgg gccacgacga gcgaggcgaa
      721 cgccagggcg aggaacacga gaaagcccgc cgccgtccgt cggaagcggc gcagcgcgtc
      781 cagcccgtcg tcccccccgg cgtcccccct gtccaccagg cgggcctagc cgccgccggc
      841 gtcgctgatg cgctcggcgc ggtcgcgcag cgcgcggaag tccttgccgc ggttgtcggc
      901 gaccagcgca tccgtcgatg agaaacccca gccttggtac ttcaccgcca cgcgcgagaa
      961 cctgcgtttc agcggcccac cgcaggcctg gcagccgtcc accggcgcgt cggcgcccag
     1021 cgcctgcagg cgctcgtcat ccgcgccaca ggtctcgcag cggtactcgt agatcggcat
     1081 cggagttctt cacagttggg gatgaccctg tggaacaaat cacaggagtg tcaccggagc
     1141 gaaccctttg tccccaagct gtggataaag ttggggatca atcacggagg tgtggttcat
     1201 caccccccgt gggtgaggtc taggtcgagc gcggtgtcag cgccactggc tgagcgtcac
     1261 caagccgccc gccatgagga tgaagcccac gatgaggttc aagttgccgc ccgggaagcc
     1321 cggcacgaag tagttgagca ggatctcgac caggcccacg ccgatgaggc cgagacccag
     1381 ccacggcacc cagcgcgggc tgggcttggg cttgggcggc ttcggcggca cgtaggtgga
     1441 ccgcttcgaa cgggacttgg gcacgactct caaccgtcct ttttgccaac agcggatgga
     1501 cactggcgcg atacgctcgc gtggcgagca tacggcagga aggccagcgg tgcgcggcga
     1561 cagcgagcgc ctgagcgagt ccccggaggc ggcggctgga cccgccgggc agccggctgc
     1621 gcccacgccg ctgacgcgtg cggccttcgc gctgaccctg gtcctgctgg tgctggtact
     1681 ggtgaccgcg gcccactccc gccccgccac gcaggacgcc cgcctgggcc gccgtgtcga
     1741 gctcgtcgag ctcatccgcg ccgagcaggc tcgcgtcaac ggcctcgcgg ccagggtgga
     1801 ggagctgtcc ggccagatcg cggcgttcga ggcccaggcc tcggccgatc ctgagctgct
     1861 ggccgcgctc caggcggaga tcgcggcgat gtccggcccc gccggcctca cgcccgtgca
     1921 gggccccggc ctcgtcgtca cgctgaccga ctcttccctg gaggagtcct cgaccggcga
     1981 cctcaacgac ctggtcatcc acgagcagga cctgcaggcc gtcatcaacg cgctgtgggc
     2041 ggggggcgcg gaggccatga gcgtcaacgg gcagcgcgtg ctggcgacca cggcgatccg
     2101 ctgcgtcggc aacaccctcc tgctgcacgg gtccgtgtac tcgccgccgt accagatccg
     2161 ctccgtgggt gatccggacg ccatgcgcgc cgagctcgcc cgcgacctgg cggtccggca
     2221 gttccagcgc gccgccgagc agttccagct cggcttctcc gtggtggagg aggaggccat
     2281 gctggtgccg gccttcgaag gcagcgccgg cctgcgctcc gcccgcgcgc tgggcgcccg
     2341 cacatgagcg cgccggaggc gatgcccccc gaggcccgcc gcgccgccat gcgcctcgtc
     2401 ggctgggccc tcgtgctcgg cggcctggga gtgctgtcct ggctggcctg gatggtgctg
     2461 gcgaccaacg ccaccaccaa ggcggcccag gaggacctcc tggaggagtg gctgcgcaga
     2521 ggcgaggccg agacccccgt gatcgacggt gcggcggcgc agccggccgc gggcgccggc
     2581 ggccccgtcg ccgtgatgga gttcctccgc gacggccggc ggatcctgca cgacgagccc
     2641 gtggtggtcg tcgccggcgt cacggcggcg gacctcacca aggggcccgg ccactatccc
     2701 ggctcggcgc tgcccggcgg ccccggcaac atggccatcg ccggccatcg caccacctac
     2761 ggcgcgccct tcgccgacat cgaccggctg gccgagggcg acgagatccg cgtggaggac
     2821 ggcgacgggg tcacgtggac ctaccgcgtc gtggagcagc gcatcgtgga gccgtccgac
     2881 accacggtca tcgccagcga cccgctcggc aacggccggc cgacgctgac gctgacgacc
     2941 tgtcacccgc gctactccaa ccgtcagcgc ctggtggtct tcgcgcaact ggctccctga
     3001 gtcgcggcgc gcgggcgccg gttggacccc ccggcggggc tgtggaacca tcccgcgcat
     3061 gagcgcgatg tcgaacaagc ccgccgtcaa gaaggcgact gccaagaagg cgaccgtcaa
     3121 gaaggcgacc gtcaagaagg cgaccgccaa gaaggcgacc gccaagaagg cgaccgccaa
     3181 gaaggcgacc gccaagcggc ggatgcccga ccccgccgag atcccacggc cgtggctggc
     3241 gtcgtaccca cccggcgtcc cggagtccta cgactacccg ctggtcccgc tgacccgctt
     3301 cctggacgac gccgcgcagg acttcccgca caccgaagcc gtggagttcc tgggttatgc
     3361 cctgacctac cgggagctgc gcgaccaggt cgaccgcctc gccacggcgc tgcacaacct
     3421 gggcgtgcgc aagggcgacc gcgtcggcat cgtgctgccc aactgcccgc agcacgtggt
     3481 cgccgtcttc gcgatcctgc gcctgggcgc cgtggtcgcg gagaacaacc cgctgtacac
     3541 ggaagccgag ctggagcacc agatcaacga cgccggctgc tcggtgctga tcgtgctgga
     3601 tcccgtgtac cagcgcatcg ccaagctgaa gggcaagctg cccagcgtcc gccacgtcat
     3661 ctccacgggc atccaggacg cgctgccgtt ccccaagaac gtgctgttcc cgctgaaggg
     3721 caaaaaggac ggcacgtact acgcgatccc cgagggcgaa ggcgtgctgc gcctcagcga
     3781 gctggtcgcg cgcacggcgc cggccgtgca gcaggccgag gtcgacccgc gtgaggatct
     3841 cgcgatgctg gtctacaccg gcggcaccac cggcgtcagc aagggcgtca tgctcagcca
     3901 cttcaacctc gtggccaacg cgttccaggc gcggctgtgg atgccggacg tgcaggcggg
     3961 gcgggagaac atcctctgcg tcctgccgtt cttccactcc tacgggctca ccacgtgcct
     4021 gaccctgggc atcctgtccg ccgcgacgct caccctcctg ccgcgcttcg accgcgacat
     4081 ggtgctgaag acgatcgaca agcggaagcc caccctgttc cccggcgtgc cgaccatcta
     4141 tgtcgccctc aacaccgcgc cggacgtgcg caagtacgac ctcacgtccg tgcgcgcctg
     4201 cctgtccggc gcggcgccgc tgccggtcga ggtcgcccgg cagttcgaag agctcacggg
     4261 cgcgaagctg cgcgagggct acggcctcac ggagacgtcc ccgatcaccc acgccaaccc
     4321 gatctacggc aaggccaaga agggttcgat cggcctgccg gtgacggaca cggtctgcgc
     4381 catcgtggac ctggacgacc ccaccaagcc ggtcgagccg ggccagcccg gctcgaccgg
     4441 cttggtgggg tcgtccaggt ccacgat
//
LOCUS       DATMKC010000117         5662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_32191, whole genome shotgun sequence.
ACCESSION   DATMKC010000117 DATMKC010000000
VERSION     DATMKC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32191"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VNU01_03480"
     CDS             complement(<1..519)
                     /locus_tag="VNU01_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31713.1"
                     /translation="MRRIWLVLVLLVALASTGAAPVASAAEETGKDCGEPRLVSDIVR
                     TGRLHARDVSPVRSVGPAPVAPCTGVRPGAAIHIPKGDTTGLCTLNFAFIGTDALKRR
                     HVYMGTAGHCLLAETNVDREKGEHHWAPGKGPVVNDPTGKRIGEFAYAILSGPKDFAL
                     IRIDYGVKLSAQM"
     gene            complement(600..1598)
                     /locus_tag="VNU01_03485"
     CDS             complement(600..1598)
                     /locus_tag="VNU01_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31714.1"
                     /translation="MPEVTPPEDWPLLFEDALACPHDPGLSVLLDHAARGAVARRDAG
                     GTFWRALDDDVAGPLLLAATPRGRELVIRGWSSVKWAGEAAPAPTGWNEGDAFHLARA
                     WVGLDDDGWGEEVLDGHPLTRELRRRLGPLRLSSVLSVQEAVGRAVLGQLVQFREAER
                     SAAQLVRLAGREVGGLHAWPTQRALLKLETWDLRRRCGMSMRGVAALRAAAFDDARLS
                     RLAREHRWDEFDAVLGALPGVGVWTSAVARLALGDPDAVPFGDYHLPSLVCHALSDGA
                     EPPGGWTDAHLAELLEPFRPQRGRVVRLLLQGAMRRQGPRPARRAPHAPLSAHRYW"
     gene            complement(1690..2610)
                     /locus_tag="VNU01_03490"
     CDS             complement(1690..2610)
                     /locus_tag="VNU01_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH31715.1"
                     /translation="MSTHRTTERTSVPEDLLDLYFSELGKVRLLTAADEVRLAKTIEA
                     GREAQERLDAGETKNTEELQRLAREGQEAFDHFVAANLRLVVSVAAQFSKRSSLDLGE
                     LIQEGNLGLLRAVEKFDWRRGYKFSTYATWWIRQAIQRGVAGSERTIRLPVALHDALV
                     KVRAASARLESETGREPTIDELAKATRMTPAKVRRAMEGDHSMTSLDRPVGYDSDASE
                     LGEFVAVASDSPAEEVVERDFLTEVFSVAREALDDRSWYVLTRRYGLDGEEALTLDSL
                     GKELGLSRESVRKIENQALARLQQELSPAA"
     gene            2869..4068
                     /gene="dapC"
                     /locus_tag="VNU01_03495"
     CDS             2869..4068
                     /gene="dapC"
                     /locus_tag="VNU01_03495"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HWH31716.1"
                     /translation="MNPLLTRLGGYPLAAFQDLKAELQAQGLPLHDFSIGDPVEPTPA
                     FIRQALIDAVPEVSQYPTAAGPPALRQAIAGWVGRRFGVTVDPDTQVLPTAGSKEAIF
                     HFPLGVLDPHSDRRAVIWGAPGYQVYERGQLFAGGTSDPVALTDERGWLLELDQLPAA
                     RLRAACIAWVNYPHNPTGAAAGLDYYERCVAAAREHGVVLASDECYMDIYPEPSADAG
                     PVDAPPPASALQAAGPDLRGVVAAFSLSKRSGMTGYRCGALVGDAELITAQRTLRPNI
                     GTASPEFVQRAAIAAWSDDAHVAARRALFDAKRAVMLDFLRQAGFRVGGSQATFYLWV
                     AAPGGDDAAYAEALLRRRIIVSPGRAFGPAGQGWFRLALVPSVEGCREAAALWAEAIG
                     AGELPGH"
     gene            4122..4943
                     /locus_tag="VNU01_03500"
     CDS             4122..4943
                     /locus_tag="VNU01_03500"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWH31717.1"
                     /translation="MTDTQRLREIIDAAFDQGTHDTPDAREAVAQALELLDRGRERIA
                     EKRDGDWVVNDWLKRAILLGFRQRGMETIEVGPFEYHDKVPLKTGYAEAGVRVVPPAV
                     VRHGAFVESGAILMPSYVNIGAWVGSGTMVDTWATVGSCAQIGRNVHLAGGVGIGGVL
                     EPPGARPVIIEDNCFVGSRAIIVEGTVIEEGAVVGAGVVITKSTQIIDVTGAEPVTLK
                     GRVPARSVVIPGTRQKEFAAGTFGVPCALIIGTRSTGTDEKVSLNDALRDYDVPV"
     gene            4954..>5662
                     /locus_tag="VNU01_03505"
     CDS             4954..>5662
                     /locus_tag="VNU01_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWH31718.1"
                     /translation="MSPLGDLLLELLALPCVTGQEGPIADWLEARYRGRGEDVVRVGH
                     SVVAGRLRDERPTVLLVGHTDVVPPTPGDLEARRADGAIHGRGASDMKSGLAVGMDCF
                     EDAALRAGPHNLVLVAYAGEEGPHDGNELGPLLDAVPALREAALAIVLEPTDLTVQLG
                     CMGALHAEVTFIGRAAHSARPWQGENALSKAGAFLTSLHERPPADVEVDGLRFREVVT
                     PTRAWTHPVREARNVVPD"
BASE COUNT          739 a         2055 c         2107 g          761 t
ORIGIN      
        1 catctgcgcg ctcagcttca cgccgtagtc gatgcggatc agcgcgaagt ccttgggtcc
       61 ggacaggatg gcgtaggcga actcgccgat gcgcttgccg gtcgggtcgt tgaccacggg
      121 gcccttgccg ggcgcccagt ggtgctcgcc cttctcgcgg tcgacgttcg tctccgccag
      181 caggcagtgg cccgccgtgc ccatgtagac gtgcctgcgc ttcagggcgt cggtgccgat
      241 gaacgcgaag ttcagcgtgc acaggccggt ggtgtcgccc ttggggatgt ggatggcggc
      301 gccgggacgc acgccggtgc acggcgccac gggcgccggg cccacggagc gcaccgggct
      361 gacgtcgcgc gcgtgcaggc ggccggtgcg gacgatgtcg gagaccagcc ggggctcgcc
      421 gcagtccttg ccggtctcct cggccgcgct cgcgaccggc gccgcacccg tcgaggcgag
      481 ggcgacaagc aggaccagga ccagccatat gcggcgcata gtgcccttta cggtagccgc
      541 ctccccgatc tgacgcagca gtccgtccga attcggtgaa gcgatgcgcg ccggcggcct
      601 caccagtagc ggtgggcgct caagggcgcg tgcggcgccc gcctggccgg ccgcgggccc
      661 tgccggcgca tggcgccctg caggagcagg cggacgacgc gcccgcgctg ggggcggaac
      721 ggctcgagca gctccgccag gtgcgcgtcg gtccagccgc cgggcggttc ggcgccgtcg
      781 gacagcgcgt ggcacaccag cgacggcagg tggtagtcgc cgaagggcac cgcgtccggg
      841 tcgcccaggg ccaggcgcgc cacggcgctc gtccacacgc ccacgcccgg cagcgcgccc
      901 agcaccgcgt cgaactcgtc ccacctgtgc tcccgcgcca gccgggacag gcgcgcgtcg
      961 tcgaacgccg cggcacgcag tgccgcaacc ccgcgcatcg acataccgca gcgccgccgc
     1021 aggtcccagg tctccagctt cagcagcgcg cgctgcgtgg gccaggcatg cagccctccg
     1081 acctcccggc ccgccaggcg caccagctgc gccgcgctgc gctcggcctc ccggaactgc
     1141 acgagctggc cgaggaccgc cctgcccacc gcctcctgga cgctcagcac gctggacagg
     1201 cgcagcggcc caagcctccg gcgcagctct cgtgtcagcg gatggccgtc cagcacctcc
     1261 tcgccccagc cgtcgtcgtc caggcccacc cacgccctgg ccagatggaa cgcatcgccc
     1321 tcgttccagc ccgtcggggc cggggcggcc tcgccggccc atttcacgct ggaccagccg
     1381 cggatgacca gctcgcgccc acggggggtc gccgccagca gcaggggacc ggcgacatcg
     1441 tcgtccagcg cgcgccagaa cgtgccacca gcgtcccttc gcgcgaccgc gccccgcgcc
     1501 gcgtggtcta gcagcacgga caggcccgga tcgtgcgggc aggccagcgc gtcctcgaaa
     1561 aggagcggcc agtcctcggg gggtgtgacc tcgggcatgg gcctagcatg ccagcgggct
     1621 gggacaggcg gccggaaagc gcgaagggca cccgggggga gggtgccctt gcgtctcggg
     1681 gtgaaggggt caggccgcgg ggctcagctc ctgctgcagc cgcgccagcg cctggttctc
     1741 gatcttgcgg acggactcgc gcgacaggcc gagctccttg cccagcgagt ccagcgtgag
     1801 cgcctcctcg ccgtccaggc cgtagcggcg cgtgaggacg taccagctgc ggtcgtccag
     1861 cgcctcgcgc gccacggaga acacctcggt gaggaagtcg cgctcgacga cctcctccgc
     1921 cggggagtcg ctggccacgg cgacgaactc gcccagctcg ctcgcgtcgc tgtcgtagcc
     1981 gaccgggcgg tccagcgagg tcatcgagtg gtcgccctcc atggcgcggc ggaccttggc
     2041 cggcgtcatg cgcgtggcct tggccagctc gtcgatggtg ggctcgcggc cggtctcgga
     2101 ctccagccgc gcggacgcgg cgcggacctt caccagggcg tcgtgcagcg cgacgggcag
     2161 gcggatggtg cgctcgctgc ccgcgacgcc gcgctggatc gcctggcgga tccaccacgt
     2221 cgcgtaggtg gagaacttgt agccgcggcg ccagtcgaac ttctccacgg cgcgcagcag
     2281 gccaaggttg ccctcctgga tcagctcgcc gaggtccagc gagctgcgct tggagaactg
     2341 cgccgccacg ctgaccacca gccgcaggtt cgccgcgacg aagtggtcga acgcctcctg
     2401 gccctcacgg gccaggcgct ggagctcctc ggtgttcttg gtctcgccgg cgtccaggcg
     2461 ctcctgcgcc tcccgccccg cttcgatggt cttggcgagc cggacctcgt ccgccgcggt
     2521 cagcaggcgg acctttccga gctcgctgaa gtacaggtcc aggaggtcct ccgggacgct
     2581 cgtacgctcg gtggttcggt gcgttgacat ggttcctgcc gattcgtcga tcgtgtgctg
     2641 gtgacaacgt gacctggggt cagtttgttc ccgcaaccgg tgcgctgccc ggggcgtttc
     2701 ccgtcctcct cgccgggtcg cggccggagt cggggaaaag tcccgagcat ctgtagacgc
     2761 tcgccgggct ccggaagttg cgggccacgg ccccgcgatg tggcttgttt cgcctccgcg
     2821 cggcgcggac cggcggtaac ggtgtccgga tggcctagca tcgggggggt gaatcccctg
     2881 ctgacgcggc tcggcggcta tccgctggcc gccttccagg acctcaaggc cgaattgcag
     2941 gcccagggct tgcccctgca cgatttctcc atcggcgacc cggttgaacc cacgccggcg
     3001 ttcatccgcc aggcgctgat cgacgccgtg cccgaggtga gccagtaccc gaccgcggcg
     3061 ggtccgccgg cgctgcgcca ggcgatcgcc ggctgggtcg gccgccgctt cggcgtcacg
     3121 gtcgacccgg acacgcaggt gctgcccacc gccggcagca aggaagccat cttccacttc
     3181 cccctgggcg tgctggaccc gcactcggac cggcgggccg tgatctgggg ggcgcccggc
     3241 taccaggtct acgagcgcgg ccagctcttc gcgggcggca ccagcgaccc cgtggcgctg
     3301 accgacgagc gcggctggct gctggagctg gaccagctcc cggccgcgcg cctgcgcgcc
     3361 gcctgcatcg cctgggtgaa ctacccgcac aacccgaccg gcgccgcggc ggggctggac
     3421 tactacgagc gctgcgtggc ggcggcgcgc gagcacggcg tcgtgctggc cagcgacgag
     3481 tgctacatgg acatctatcc ggaaccatcc gctgacgcgg ggccggtgga cgccccgccg
     3541 cccgcgtcgg cgctccaggc cgcgggcccg gacctgcgcg gcgtggtggc ggcgttcagc
     3601 ctgtcgaagc gctccggcat gacggggtat cgctgtggcg cgctggtcgg cgacgcggag
     3661 ctgatcacgg cgcagcgcac gctgcggccc aatatcggca cggccagccc cgagttcgtg
     3721 cagcgggcgg ccatcgcggc gtggtccgac gacgcgcacg tggccgcccg gcgcgcgctg
     3781 ttcgacgcca agcgcgcggt gatgctggac ttcctgcgcc aggccggctt ccgcgtcggc
     3841 ggcagccagg cgacgttcta cctgtgggtg gcggcccccg gaggcgacga cgccgcctac
     3901 gccgaggcgc tgctgcgccg gcgcatcatc gtctcgcccg gccgcgcgtt cggcccggcc
     3961 ggacaaggct ggttccggct ggcgctcgta ccctccgtgg aaggatgccg ggaggccgcc
     4021 gccctgtggg ccgaggcgat cggcgccggg gagctgccgg ggcactgacg ggccccggcg
     4081 cgaagacgat gcccaggaca acgccaaggg agactgacga cgtgaccgac acgcagcgcc
     4141 tgcgcgagat catcgacgcg gcgttcgacc aggggacgca cgacacgccc gacgcccgcg
     4201 aggcggtggc gcaggcgctg gagctgctcg accgcggccg ggagcgcatc gccgagaagc
     4261 gcgacggcga ctgggtggtc aacgactggc tgaagcgcgc gatcctgctc ggcttccgcc
     4321 agcgcggcat ggagaccatc gaggtcgggc cgttcgagta ccacgacaag gtgccgctga
     4381 agaccggcta cgccgaagca ggcgtgcgcg tggtgccgcc cgcggtggtg cgccacggcg
     4441 cgttcgtgga gtccggcgcc atcctgatgc ccagctacgt caacatcggc gcgtgggtcg
     4501 gcagcggcac gatggtggac acctgggcga cggtcggctc gtgcgcgcag atcgggcgca
     4561 acgtgcacct ggcgggcggg gtcggcatcg gcggcgtcct ggagccgccg ggcgcccggc
     4621 ccgtgatcat cgaggacaac tgtttcgtcg gctcgcgcgc gatcatcgtg gagggcaccg
     4681 tgatcgagga gggcgccgtc gtgggcgccg gcgtggtgat caccaagtcc acgcagatca
     4741 tcgacgtgac cggcgcggag cccgtcaccc tgaaggggcg ggtgcccgcg cgctccgtcg
     4801 tgatccccgg cacgcggcag aaggagttcg ccgcggggac gttcggcgtg ccgtgcgcgc
     4861 tgatcatcgg cacgcgctcg accggcaccg acgagaaggt gtccctgaac gacgcgctgc
     4921 gcgactacga cgtaccggtc taggcggaag cgcatgagcc ccctcggcga cctgctgctg
     4981 gagctgctgg ccctaccctg cgtgacgggc caggagggcc ccatcgcgga ctggctggag
     5041 gcccgctacc gcggccgggg cgaggacgtg gtgcgggtgg gccattcggt ggtcgccggg
     5101 cgcctgcgcg acgagcggcc gaccgtgctg ctggtcggcc acaccgacgt ggtcccgccg
     5161 acgccgggcg accttgaggc ccgccgtgcc gatggcgcga tccacggccg cggcgccagc
     5221 gacatgaagt cgggcctggc cgtgggcatg gactgcttcg aggacgcggc cctgcgcgcg
     5281 gggccgcaca acctggtgct ggtggcctac gcgggggagg aggggcccca cgacggcaac
     5341 gagctcggtc cgctgctgga cgcggtgccg gcgctgcggg aggcggccct ggcgatcgtg
     5401 ctcgagccga cggacctcac cgtgcagctg ggctgcatgg gggcgctgca cgccgaggtg
     5461 accttcatcg gcagggcggc gcactccgcc cgcccctggc agggcgagaa cgcgctgagc
     5521 aaggcgggcg ccttcctgac atcgctgcac gagcggccac cggcggacgt cgaggtggac
     5581 ggcctgcgct tccgcgaggt cgtcacaccg acgcgcgcgt ggacccaccc ggtgcgcgag
     5641 gctcgcaacg tggtgccgga cc
//
LOCUS       DATMKC010000118         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_279523, whole genome shotgun sequence.
ACCESSION   DATMKC010000118 DATMKC010000000
VERSION     DATMKC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279523"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VNU01_03510"
     CDS             <1..509
                     /locus_tag="VNU01_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWH31719.1"
                     /translation="VAQLDEFGRLVGGLVELARGARPAGAVTPVRLDELVGRVASSAR
                     LGPGAPPVSLDAAPTTVLGEEDRLERAVANLVDNAVKYGGGGPVEVTVRDGTVVVRDH
                     GPGIPPEHLPHVFDRFYRAPETRAAPGSGLGLAIVRQVADAHGGTAHAANAPDGGAVL
                     TLRLPVKG"
     gene            complement(562..1083)
                     /locus_tag="VNU01_03515"
     CDS             complement(562..1083)
                     /locus_tag="VNU01_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH31720.1"
                     /translation="MGITRLNHAVLYVRDLDRSVAFYTRVLGFRVVPIAPAGFRAAFL
                     QAAGSDNDHDLGLFEVGAGAGPSAAGRQTVGLYHLAWEVDTLEELERIQGELGRAGAL
                     GGMSDHGTSKSLYGHDPDGLEFEVAWFLPEELVDAEILAARKSIRPLDLGREAARYGA
                     TTPGRNAPQRPGP"
     gene            1211..1672
                     /locus_tag="VNU01_03520"
     CDS             1211..1672
                     /locus_tag="VNU01_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWH31721.1"
                     /translation="MTEPRWLDEDEQRAWRAFLAASRLLFDRLERELQHDAGMPHAYY
                     EILVRLSEAPDRRLRMSELADKSLSSRSRVSHAARRLEEAGWLRREECETDRRGAWAV
                     LTDAGYAVLQAAAPGHVEGVRTHLFDQLSAEQVAQLRQISEAVVAHLEPLD"
     gene            complement(1694..1912)
                     /locus_tag="VNU01_03525"
     CDS             complement(1694..1912)
                     /locus_tag="VNU01_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31722.1"
                     /translation="MDSWTDRCGHCGRGIARVGRAQSYVHANQFHMRFDHPAEPARHT
                     RPEPLAVGGHWGPDRGRIVRRPSHLRVA"
BASE COUNT          275 a          762 c          786 g          275 t
ORIGIN      
        1 tcgtcgcgca gctggacgag ttcggccggc tggtcggcgg cctggtggag ctggcgcgcg
       61 gcgccaggcc cgccggcgcg gtcacgcccg tgcgcctcga cgagctggtg ggccgcgtgg
      121 cctcgtcggc aaggctgggg cccggcgcgc cgccggtcag cctggatgcc gcgccgacca
      181 ccgtgctggg cgaggaggac cggctggagc gcgccgtcgc gaacctggtc gacaacgcgg
      241 tgaagtacgg cggcggcggg ccggtggagg tcaccgttcg ggacggcacg gtggtcgtgc
      301 gggaccacgg cccgggcatc ccgcctgagc acctgccgca cgtcttcgac cgcttctacc
      361 gcgcgccgga gacacgcgcc gcgccggggt ccggactggg gctcgccatc gtgcgccagg
      421 tggccgacgc gcacggcggc acggcgcacg ccgccaacgc gccggacggc ggcgcggtcc
      481 tgaccctgcg cctgcccgtc aaaggttgaa ccgttcgtcc gagatctcgg acgaacggtt
      541 ccaggttggg aagggaggcg gtcagggacc ggggcgttgc ggggcgttgc ggcccggcgt
      601 cgtggccccg tagcgggcgg cctcgcgacc caggtccagc ggccggatgc tcttgcgggc
      661 cgccaggatc tccgcgtcga ccagctcctc cggcaggaac caggccacct cgaactccag
      721 gccgtcgggg tcgtggccgt acaggctctt ggacgtgccg tggtcgctca tgccgcccag
      781 cgcgcccgcc cggccgagct cgccctggat gcgctccagc tcctccagcg tgtcgacctc
      841 ccacgccagg tggtacaggc cgacggtctg gcggcccgcg gccgacgggc ccgctccggc
      901 accgacctcg aacaggccca ggtcgtggtc gttgtccgag ccggccgcct gcaggaacgc
      961 ggcacggaac cccgccggcg ctatcggcac cacgcggaag cccagcaccc gcgtgtagaa
     1021 ggcgacgctg cggtccaagt cccgcacgta cagcaccgcg tggttgagcc tcgtgatgcc
     1081 catcccgatc actccctcgt atcgtggtcc ctgcgcggga cagtctcgcc ccgcacggca
     1141 tggaacaaag ttgagtgctc aactgttccc tggagggtga accgagggcg gaaccccaag
     1201 gggggaaccc atgactgaac cacgctggct ggacgaggac gagcagcgcg cctggcgcgc
     1261 gttcctcgcg gcgtcccgcc tgctgttcga ccgcctggag cgcgagctcc agcatgacgc
     1321 cggcatgccg cacgcctact acgagatcct ggtgcgcctg tcagaggctc cggatcgccg
     1381 gctgcgcatg agcgagctcg ccgacaagtc gctgtcctcg cgcagccggg tgtcccacgc
     1441 cgcgcggcgg ctggaggaag cgggctggct gcgccgagag gagtgcgaga cggaccgccg
     1501 gggcgcgtgg gcggtgctca ccgacgccgg ctacgccgtc ctgcaggctg ccgcgcccgg
     1561 gcacgtcgag ggggtccgca cgcacctgtt cgaccagctc tcagccgagc aggttgcgca
     1621 gctgcgccag atcagcgaag ccgtggtggc ccacctggag ccgctggact gagacgcgcg
     1681 gacgcgcgtg cgctcaggcg acgcgcaggt gggacggccg ccgcacgatg cggccgcggt
     1741 ccgggcccca atgcccgccc acggcgagcg gctccgggcg ggtgtgccgc gcgggctcgg
     1801 ccgggtggtc gaagcgcatg tggaactggt tggcgtgcac gtaggactgc gccctgccga
     1861 cgcgagcgat gccgcggccg cagtggccgc accggtccgt ccagctgtcc atcgctgacc
     1921 cctcacgcca gtgcatgact cctggagcaa gtccatccca cgatgcggcg acgcgcagtc
     1981 ccccatcggg actgaatcgg ccgcacgtcc tgactagtca gcgcgccggc ggcggcggcg
     2041 ggtacggggg gcggcggaat ttgcgtggcg ggtcctcccc ttccggcccg gcctgggc
//
LOCUS       DATMKC010000119         6057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_226344, whole genome shotgun sequence.
ACCESSION   DATMKC010000119 DATMKC010000000
VERSION     DATMKC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226344"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..670)
                     /gene="nusB"
                     /locus_tag="VNU01_03530"
     CDS             complement(227..670)
                     /gene="nusB"
                     /locus_tag="VNU01_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWH31723.1"
                     /translation="MAPGDERDEPRVPRGSRHEARRRALEILYEADLKERPVPTILAA
                     HLAGDEQPPEYALALVRGVHRGIARIDELITTHAKGWTLGRMPIVDRNLLRIAVHELL
                     DVEDVPTAVAINEAVELAKELSTDDSGRFINGVLARIAEEGPAAP"
     gene            complement(670..1227)
                     /gene="efp"
                     /locus_tag="VNU01_03535"
     CDS             complement(670..1227)
                     /gene="efp"
                     /locus_tag="VNU01_03535"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWH31724.1"
                     /translation="MVSTNNLKNGMTVKIDGELWRVDYFQHVKPGKGGAFVRTTLKGV
                     QNGKTIDRTFRAGEELEQAILEKRSLQYLYREGDSFVFMDTEHYEQSHVPEDAVREAA
                     KWIKEGDTIDLVFHDGEVVDVNLPANVTLTITQTEPGVQGDRVSGATKPATLETGAVV
                     QVPLFINEGEAIKVDTRSAEYISRA"
     gene            complement(1265..2353)
                     /locus_tag="VNU01_03540"
     CDS             complement(1265..2353)
                     /locus_tag="VNU01_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWH31725.1"
                     /translation="MPQSAHAARRERLRARLADHGLAGYLVTRLVNVRYLTGFTGSNG
                     QVLVSARASGDVFATDGRYDEQAAAEAGDLPRVLAREEDWLPERLAAGDRVGFESHDV
                     SWERGQALSAALRERAGVELVAAPRWVEELREVKDASEVAALRRACALADAAFAGLLE
                     WLRPGMTERDVAGRLEQAMREGGADGPSFETIVASGPNSARPHHRPTSRAIASGDVVK
                     VDFGALVDGYHSDMTRTVAVGEPDAALRGVHDLVRRAQAAGRAAALAGAPASAVDAAC
                     REAIAAAGHGERFVHPTGHAVGLEIHEQPILHGAATATLRAGMAVTVEPGVYLPGVGG
                     VRIEDVVVVADQGPPEVLTTSPRELLVL"
     gene            complement(2672..3091)
                     /locus_tag="VNU01_03545"
     CDS             complement(2672..3091)
                     /locus_tag="VNU01_03545"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWH31726.1"
                     /translation="MTVLLLHGPNLSQLGTRDPAHYGTATLADVEAAVRAVLGDVDAF
                     QSEHEGDLVARLHAARGDGTTAVIANPGALTHYSYALRDALELLEVPKVEVHLSQTHA
                     REEWRRHSVVSPVVDVTITGAGVTGYRLAAEYVQSRL"
     gene            complement(3088..4170)
                     /gene="aroB"
                     /locus_tag="VNU01_03550"
     CDS             complement(3088..4170)
                     /gene="aroB"
                     /locus_tag="VNU01_03550"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWH31727.1"
                     /translation="MTGPIRVDLPGRGYDIVVEAGALGRLPELLGPPPHARRAALVTN
                     AAVAALYEAPVVAGLQSLGLDVQVVTVPDGEDAKSVATLAMLWHRFAGMPLNRDDLVV
                     ALGGGVVGDLAGFAAATWNRGIAVAQLPTTLLAMVDAAIGGKTGINLPEGKNLVGAFH
                     QPLVVLADTATLTTLPPRELRAGLGEVAKYGFISDPLVLELLEKDPQAAQAGDPALLT
                     EVVRRGAAVKARIVAADERESGERALLNYGHTVGHAIESLTGYDSYRHGEAVGLGMVF
                     AARLGERIGVSEPGLAERTVAVLDALGLPTGGVRLEPSAVWDVLARDKKARAGVRFVL
                     CRTPGEALLVEQPERRIVDEVLRSLA"
     gene            complement(4167..4745)
                     /locus_tag="VNU01_03555"
     CDS             complement(4167..4745)
                     /locus_tag="VNU01_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWH31728.1"
                     /translation="MSGDGARNIVLGGLMGTGKTTLARRLAVRLGRQWLDTDALVAQR
                     AGCSIAQLFARRGEEAFRELEREVVAEAAELRRHIVSLGGGVLLDGGNVELLRAGGVV
                     VLLVGDVAVLAERVTRAGVARRPLLAGETDVHGRLERLAGERRVAFEAAADHVEDTTD
                     GRSADAIAASIVAWAATRPDVLTPGERKAVAA"
     gene            complement(4742..5944)
                     /gene="aroC"
                     /locus_tag="VNU01_03560"
     CDS             complement(4742..5944)
                     /gene="aroC"
                     /locus_tag="VNU01_03560"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWH31729.1"
                     /translation="MTLRYLTAGESHGAALVGILEGMPAGVPVDRARIERELERRRLG
                     HGRSPRMNFEQDRLEILGGVVHGRTLGSPIAIVVHNSEWPKWTEVMSPDPVENPEALR
                     ETGRGEPLTRPRPGHADLAGMQKYGFDEARPVLERASARETAARVGLACFGRFLLEEL
                     GIRVLSHVVRIGEVAVAEDAPLPGPDDLERVDADPVRCFDADASARMVERIDRAKGEG
                     DTLGGVVEVVVHGLPPGLGSHVHWDRKLDARLAAALMSIQSVKGVEAGEGFRSAEVPG
                     SKAHDEIVPDSERGYRRVSGRSGGIEGGMSTGEVLRVRAAMKPISSIPRPLRTVDVRS
                     GDPAEAIHQRSDACAVPRGAVVAEHVVALELAGALLEKTGGDSLEEVRRNLDGYVSSL
                     TPPWLGRP"
BASE COUNT          761 a         2375 c         2126 g          795 t
ORIGIN      
        1 cccagcggca gcgccttcgc gcgacgcaac acgcgctcgg ccacgtccag cagaccgtga
       61 aggtccgcgg tggccagcgc cgcgtcgatc ccgaaccccg cactcgaaca catgttcgac
      121 accatacaac gcgactgcga caggtcaacc cacgaaattg aatctgtggg aaagatgcct
      181 tgacgacgaa gtcctcggac cgccggacac gaacacggca accgcgtcag ggagcggcgg
      241 ggccttcctc ggcgatccgc gccagcaccc cgttgatgaa gcgccccgag tcgtcggtgg
      301 acagctcctt ggccagctcc accgcctcgt tgatcgccac ggccgtgggc acgtcctcca
      361 cgtccagcag ctcgtgcacg gcgatgcgca gcaggttgcg gtcgacgatg ggcatgcggc
      421 ccagcgtcca gcccttcgcg tgcgtggtga tcagctcgtc gatgcgggcg atgccgcggt
      481 gcaccccgcg caccagcgcc agggcgtact cgggtggctg ctcgtcgccg gccaggtgcg
      541 cggccagaat cgtcgggacc gggcgctcct tcagatcggc ctcgtacagg atctccaggg
      601 cgcggcggcg cgcctcgtgc cggctgcccc gcgggacgcg gggctcgtcc cgctcgtcgc
      661 ctggagccat caggcgcgcg agatgtactc ggcactgcgc gtgtcgacct tgatggcctc
      721 gccctcgttg atgaacagcg gcacctgcac cacggcgccc gtctccagcg tggccggctt
      781 ggtggccccc gacacgcggt cgccctgcac gccgggctcg gtctgcgtga tggtcagcgt
      841 gacgttggcc ggcaggttca cgtccaccac ctcgccgtcg tggaacacca ggtcgatggt
      901 gtcgccctcc ttgatccact tggcggcctc gcgcaccgcg tcctcgggca cgtgcgactg
      961 ctcgtagtgc tcggtgtcca tgaacacgaa cgagtcgccc tcgcggtaca ggtactgcag
     1021 cgagcgcttc tccaggatgg cctgctcgag ctcctcgccc gcgcggaacg tccggtcgat
     1081 ggtcttgccg ttctgcacgc ccttcagcgt ggtgcgcacg aaggcgccgc ccttgcccgg
     1141 cttgacgtgc tggaagtagt cgacgcgcca cagctcgccg tcgatcttga ccgtcatgcc
     1201 gttcttgagg ttgttcgtcg agaccatggc tgcttccgac ttcctgatcg ggtgcggcgc
     1261 cgcgctacag cacgagcagc tcgcggggac tggtggtgag gacctccggc ggcccctggt
     1321 cggcgaccac caccacgtcc tcgatgcgga cgccgcccac gccggggagg tagacgcccg
     1381 gttcgacggt gaccgccatg ccggcgcgga gtgtagcagt ggccgcgccg tgcaggatcg
     1441 gctgctcgtg gatctccagc cccaccgcgt gcccggtggg gtgcacgaag cgctccccgt
     1501 ggccggctgc cgcgatcgcc tcccggcagg cggcgtccac cgccgaggcg ggggcgcccg
     1561 ccagcgcggc cgcgcgcccg gcggcctgcg cccggcgcac caggtcgtgc acgccccgca
     1621 gcgccgcgtc cggctcgccc accgcaacgg tccgcgtcat gtcgctgtgg tagccgtcca
     1681 ccagcgcgcc gaagtccacc ttcaccacat cgccggaggc gatcgcccgg gacgtcggcc
     1741 ggtggtgcgg ccgcgcgctg ttgggcccgg acgccacgat ggtctcgaac gacggcccgt
     1801 cggccccgcc ctcgcgcatg gcctgctcca gccggcccgc cacgtcacgc tccgtcatgc
     1861 cggggcgcag ccactccagc agcccggcga acgcggcgtc cgccagggca caggcgcgcc
     1921 gcagcgccgc cacctccgag gcgtccttga cctcgcgcag ttcctccacc cagcggggcg
     1981 ccgccaccag ctccacaccg gcgcgctcgc gcagcgccgc cgacagcgcc tggccgcgct
     2041 cccacgacac gtcgtggctc tcgaagccca cccggtcgcc ggccgccagg cgctccggca
     2101 gccagtcctc ctcgcgcgcc agcacgcgcg gcaggtcgcc cgcctccgcg gccgcctgct
     2161 cgtcgtagcg gccgtcggtg gcgaacacgt ccccggacgc ccgcgcgctc accagcacct
     2221 gcccgttgct gcccgtgaag ccggtcaggt agcggacgtt gaccaggcgc gtgaccaggt
     2281 accccgccag gccgtggtct gcgaggcggg cgcggaggcg ctcccggcgg gcggcgtggg
     2341 ctgattgggg catggcgact cactgtaacg gtcgggcgcg gcgcgccccg cccgggtgaa
     2401 caggggtcta cgaccggcct acgacgcacg gttgacttct caccctgcgg aatcgccccg
     2461 ctcacccgta cgggggaaga actgtgcggg caatgcccct agcttctggc ccaggagacg
     2521 gggggacagc cgagctgtcc aggtttccaa acgtccaccc cgccggtccc tggcgctccc
     2581 cggggtcggc ggggtggacc ctttctcgga ggcgatcgcg tacctgcgcg cgccttcggc
     2641 gcgctgaccg cgatcggccg gcccctcggg tctacaggcg ggactgcacg tactcggcgg
     2701 ccaggcggta gccggtgacg cccgcgcccg tgatggtcac gtcgaccacg gggctcacca
     2761 cgctgtggcg gcgccactcc tcgcgcgcgt gggtctggga caggtgcacc tccaccttgg
     2821 gcacctccag gagctccagc gcgtcgcgca gcgcgtagct gtagtgcgtc agcgccccgg
     2881 ggttggcgat caccgcggtg gtgccgtcgc cgcgcgcggc gtgcagccgg gcgaccaggt
     2941 cgccctcgtg ctcggactgg aacgcgtcca cgtcccccag caccgcgcgc accgccgcct
     3001 cgacgtcggc cagcgtggcc gtgccgtagt gggccgggtc gcgcgtgccg agctgcgaca
     3061 ggttggggcc gtgcagcaac aggacggtca tgccaggctc ctcagcacct cgtcgacgat
     3121 gcggcgctcc ggctgctcga ccagcagcgc ctcccccggc gtccggcaca gcacgaagcg
     3181 cacaccggcc cgggccttct tgtcccgcgc gagcacgtcc cagaccgccg agggctccag
     3241 gcgcacgccg ccggtcggca ggcccagcgc gtccagcacc gcgacggtgc gctcggccag
     3301 gccgggctcg ctcacgccga tgcgctcccc cagccgggcg gcgaacacca tgccgaggcc
     3361 gacggcctcg ccgtggcggt agctgtcgta gccggtgagc gactcgatcg cgtgccccac
     3421 ggtgtgcccg tagttgagca gcgcccgctc gccggactcg cgctcgtccg cggcgacgat
     3481 gcgcgccttc accgcggcgc cgcggcgcac cacctccgtg agcagcgccg ggtcgccggc
     3541 ctgcgcggcc tgggggtcct tctccagcag ctccagcacc agcgggtcgg agatgaagcc
     3601 gtacttggcc acctcgccca gcccggcccg cagctcgcgg ggcggcagcg tggtcagcgt
     3661 cgccgtgtcg gccagcacga cgagcggctg atggaacgcg ccgaccaggt tcttgccctc
     3721 cggcaggttg atgccggtct tgccgccgat ggcggcgtcg accatggcca gcagcgtggt
     3781 gggcagctgc gccacggcga tgccgcggtt ccacgtcgcc gcggcgaagc ccgcgaggtc
     3841 ccccaccacg ccgccgccga gcgcgaccac caggtcgtcg cggttcagcg gcatgccggc
     3901 gaagcggtgc cacagcatcg ccagggtggc gaccgacttc gcgtcctcgc cgtccggcac
     3961 ggtgaccacc tggacgtcca ggcccaggga ctgcaggccg gcgaccacgg gcgcctcgta
     4021 cagcgccgcc acggccgcgt tggtgaccag cgcggcgcgc cgggcgtgcg gcggcgggcc
     4081 gagcagctcc ggcaggcgcc ccagggcgcc ggcctccacg acgatgtcgt agccgcgccc
     4141 cggcaggtcc acgcggatgg gcccggtcac gccgccaccg ccttgcgctc gccgggcgtc
     4201 agcacgtcgg gccgggtcgc cgcccaggcg acgatgctcg cggcgatggc gtcggcggag
     4261 cggccgtccg tggtgtcctc gacgtggtcg gcggcagcct cgaaggccac ccgccgctcg
     4321 cccgccaggc gctccagccg cccgtgcacg tcggtctcgc cggccagcag cggccggcgc
     4381 gccactcccg cgcgcgtcac gcgctcggcc agcaccgcca cgtcgccgac caggagcacc
     4441 acgacgcccc cggcgcgcag cagctcgacg ttgcccccgt ccagcagcac gccgccgccc
     4501 agggacacga tgtgacggcg cagctcggcg gcctccgcga ccacttcccg ctccagctcc
     4561 cggaacgcct cctcgccgcg gcgcgcgaac agctgcgcga tcgagcagcc ggcgcgctgg
     4621 gcgaccagcg cgtcggtgtc cagccactgc cgccccaggc gcacggccag ccgccgcgcc
     4681 agcgtggtct tgccggtgcc catcagcccg ccgagcacga tgttgcgcgc cccgtcgccg
     4741 ctcacggccg gccgagccag ggcggcgtca gggaggacac gtagccgtcg aggttgcgcc
     4801 ggacctcctc cagcgagtcc ccgccggtct tttccagcag cgccccggcg agctccagcg
     4861 ccaccacgtg ctcggcgacc acggcgccgc gcggcaccgc gcacgcgtcg ctgcgctggt
     4921 ggatcgcctc cgcggggtcg ccgctgcgca cgtccacggt gcgcaggggc cgcgggatgc
     4981 tggagatggg cttcatggcg gcgcgcacgc gcagcacctc gccggtggac atgccgcctt
     5041 cgatgccgcc ggagcgtccg ctcacccggc ggtagccgcg ctcgctgtcc ggcacgatct
     5101 cgtcgtgcgc cttggagccg ggcacctcgg cggaacggaa gccctcgccg gcctccacgc
     5161 ccttcaccga ctggatgctc atcagcgccg cggccaggcg cgcgtccagc ttgcggtccc
     5221 agtgcacgtg gctgcccagg cccggcggca gcccgtggac cacgacctcc accacgccgc
     5281 ccagcgtgtc gccctcgccc ttggcgcggt cgatgcgctc gaccatgcgc gcggaggcgt
     5341 cggcgtcgaa gcagcgcacc gggtccgcgt cgacgcgctc caggtcgtcc gggcccggca
     5401 ggggcgcgtc ctcggcgacg gcgacctcgc cgatgcgcac gacgtgcgac agcacgcgga
     5461 tgccgagctc ctccagcagg aaccggccga agcaggccag gcccacgcgg gcggcggtct
     5521 cccgcgcgct cgcgcgctcc aggacggggc gcgcctcgtc gaagccgtac ttctgcatgc
     5581 cggccaggtc cgcgtggccg ggacggggcc gggtgagcgg ctcgccgcgc ccggtctccc
     5641 gcagggcttc cgggttctcg accgggtccg ggctcatcac ctcggtccac tttggccact
     5701 cgctgttgtg caccacgatg gcgatcggcg agccgagggt gcggccgtgc accacgccgc
     5761 ccaggatctc caggcggtcc tgctcgaagt tcatccgcgg cgagcggccg tgccccaggc
     5821 ggcggcgctc cagctcgcgc tcgatgcgcg cgcggtcgac gggcacgccg gcgggcatgc
     5881 cctccaggat gccgaccagc gcggccccgt gggactcacc ggccgtcaga taacgaagcg
     5941 tcatggtccc gagaaggtac cagcagcgac gccgcctggg gcacggccgc tcgccgcgga
     6001 tgaacttcga gcaggaccgc ctggagatcc tgggcggcgt ggtgcacggc cgcaccc
//
LOCUS       DATMKC010000120         3361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_5039, whole genome shotgun sequence.
ACCESSION   DATMKC010000120 DATMKC010000000
VERSION     DATMKC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5039"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VNU01_03565"
     CDS             <1..772
                     /locus_tag="VNU01_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH31730.1"
                     /translation="TARPSPPGRLSTALTFGWRGMLKVKHVPEQLLDVTITPVMFVLM
                     FTYLFGGAVAGSTAAYLDYILPGILVMSVLFTTVYSGIALNTDLTKGVVDRFRSLPIW
                     RPAPLVGSLLGDSVRYVLAGTVIITVGVILGYRPGAGVLGAMSALGLVVLFAFGLSWV
                     FTTMGLVLRTPNAVMNAGFMGIFPLTFLSNVFVDPRTLPGPLEAFVKVNPISILATAS
                     RGLMEGNVATSDILTVLAVAAGLTAVFAPLTTRLYRSR"
     gene            complement(800..1723)
                     /locus_tag="VNU01_03570"
     CDS             complement(800..1723)
                     /locus_tag="VNU01_03570"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor RsbA family regulatory
                     protein"
                     /protein_id="HWH31731.1"
                     /translation="MTAATLRHDALLYDSDASFVSALAPFVCEGVDAGEGVMVVTARP
                     RIEALRDELGRDADDVSFVPAADWYVHPVTTIAAWADALRSLRERGHPVTRVVGEVQF
                     GDTEEARHSWVRYESALNAVFADSDTWIVCPYDARVLGTDLLEAAARTHPAVWHGRDR
                     RPSADYVEPGRLLRDVPEPWPAPPGPPVLRAEFTDGLVSLRHDVRAAVGRALPPDRLE
                     DALVVLTELVTNALRHGAPPALVTVWARPGEVVAEVADHGVAGLDPLAGYHPPPPGSS
                     NGMGLWVARHLSDRLAVRSGAGTVVRVAVHG"
     gene            complement(1720..2595)
                     /locus_tag="VNU01_03575"
     CDS             complement(1720..2595)
                     /locus_tag="VNU01_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HWH31732.1"
                     /translation="MPNESGDSREPATLAPGDHACWAFGGVDSWRDTVAAYLIAGRLA
                     GERLLLLGTPQRTGQALDALDALGMDSAGLRAEGALVVADAEAAYLPGGAFDADARIA
                     VYERLVLAALADGYTGLRVAADVPPALREITASGSWPSYELRADLLAARLPFTALCCY
                     DRQWWAPDDVAPIAAVHALAFGPEGAAGHCGFRLHSGPDGAIALDGELDMAGSELLGR
                     LLRSASAEMSGGLLDVSGLRFVDIVGLRAVADAAIALADLTSRPAVIRGASPAFVRLW
                     HLLGLQEECRVKVAA"
BASE COUNT          459 a         1218 c         1208 g          476 t
ORIGIN      
        1 cacggcccgg ccgtccccgc ccgggcgcct gtccacggcg ctcacgttcg gctggcgcgg
       61 catgctcaag gtgaagcacg tgccggagca gctgctggac gtgaccatca cgccggtgat
      121 gttcgtgctg atgttcacgt acctgttcgg cggcgccgtg gccggctcga ccgccgcgta
      181 cctggactac atcctgcccg gcatcctggt gatgtcggtg ctgttcacca cggtctactc
      241 cggcatcgcg ctgaacaccg acctcaccaa gggcgtggtc gaccggttcc gctcgctgcc
      301 catctggcgg ccggcgccgc tggtcggctc gctgctcggc gacagcgtgc gctacgtgct
      361 ggccggcacg gtgatcatca ccgtcggcgt gatcctgggc taccgcccgg gcgccggcgt
      421 cctgggcgcc atgtccgcgc tgggcctcgt ggtgctgttc gcgttcgggc tgtcgtgggt
      481 gttcaccacg atgggcctgg tgctgcgcac gcccaacgcg gtcatgaacg ccgggttcat
      541 gggcatcttc ccgctgacgt tcctgtccaa cgtgttcgtg gaccccagga cgctgcccgg
      601 gccgctcgag gcgttcgtca aggtgaaccc gatctcgatc ctggccaccg cgtcgcgcgg
      661 cctgatggag ggcaacgtcg cgacgtcgga catcctgacc gtcctggcgg tcgcggccgg
      721 actcaccgcg gtcttcgccc cgctcaccac gcgcctgtac cgcagccgct gaacctgccg
      781 cggccgctcc gcggcgggct cagccgtgca cggcgacgcg cacgaccgtg ccggcaccgc
      841 tgcggacggc caggcggtcc gacaggtggc gcgccaccca cagtcccatg ccgttggacg
      901 acccgggcgg cggcgggtgg taccccgcca gggggtccag tcccgcgacg ccgtggtcgg
      961 cgacctcggc gaccacttcg cccgggcgtg cccacaccgt caccagggcc ggtggggcgc
     1021 cgtgacgcag ggcgttggtg acgagctccg tcaggacgac gagcgcgtcc tccaggcggt
     1081 cgggaggcag ggcgcggccc acggcggcgc gcacgtcgtg gcgcaggctg accagcccgt
     1141 cggtgaactc cgcgcggagc accggcgggc ccggcggcgc cggccacggc tcgggcacgt
     1201 cgcgcagcag gcgcccgggc tccacgtagt ccgcgctcgg gcgccggtcg cggccgtgcc
     1261 acacggcggg gtgggtgcgg gccgccgcct ccagcaggtc ggtcccgagc acccgggcgt
     1321 cgtaggggca cacgatccag gtgtcgctgt cggcgaagac ggcgttcaag gcggactcgt
     1381 agcggaccca ggagtggcgc gcctcctcgg tgtcgccgaa ctgcacctcg cccaccacgc
     1441 gcgtgacggg gtggccgcgc tcgcgcagcg agcgcagcgc atcggcccag gcggcgatcg
     1501 tggtgacggg gtggacgtac cagtcggcgg cgggcacgaa cgagacgtcg tcggcgtcgc
     1561 ggccgagttc gtcgcgcagc gcctcgatgc gcggccgtgc ggtgaccacc atgacgccct
     1621 cgccggcgtc cacgccctcg cagacgaatg gggcgagcgc ggacacgaag gaggcgtcgc
     1681 tgtcatagag cagcgcgtca tggcgcaggg tcgcggccgt catgcggcga ccttcacgcg
     1741 gcattcctcc tgcagtccca gcaggtgcca caagcgcacg aacgccggtg acgccccgcg
     1801 gatgaccgcc gggcgggacg tcaggtcggc cagcgcgatg gcggcatcgg cgaccgcacg
     1861 gagcccgacg atgtcgacga accgcaggcc gctcacgtcc agcagcccgc cggacatctc
     1921 agccgacgcg ctgcgcagga ggcggccgag cagctcggag cccgccatgt ccagttcgcc
     1981 gtccagcgcg atggcgccgt ccgggccgct gtgcagacgg aagccacaat ggccggcggc
     2041 gccctccggc ccgaacgcga gcgcgtggac ggcggcgatg ggcgcgacgt cgtccggggc
     2101 ccaccactgc cggtcgtagc agcacagtgc cgtgaacggc agtcgcgcgg cgagcaggtc
     2161 cgcgcgcagc tcgtacgacg gccaggagcc ggacgcggtg atctcgcgca gagcgggcgg
     2221 cacgtccgcg gcgacgcgga gcccggtgta gccgtccgcc aaggccgcca ggacgagccg
     2281 ctcgtacacg gcgatcctcg cgtcggcgtc gaaggcgccg ccgggcaggt atgccgcttc
     2341 cgcgtccgcc accacaaggg cgccctctgc ccgcaagccc gcgctgtcca tcccgagggc
     2401 gtccagcgcg tccagcgcct ggccggtgcg ctgcggggtg ccgagcagca ggaggcgctc
     2461 accggcgagg cgcccggcga tcaggtacgc ggcgaccgtg tcccgccacg agtccacccc
     2521 gccgaacgcc cagcacgcgt gatcgccagg cgccagtgtg gctggttcgc ggctgtcgcc
     2581 cgattcgttc ggcatctgcc gactgtagcc cagcacccga cggccgggaa gggcacgcgc
     2641 ggcgcggccg ggaacccgac cagtccgcca cgtcgtggtg tgcgcctcct acgacttcca
     2701 gtcgtcgccc ggcatggtgc ggcgcatgaa ctcgtcgaaa agcggcacgg tgaacgcggt
     2761 atcgccgtgg ctggggctcc acgaccgatg aggcccggga ccaagttccg ccatcgctcg
     2821 caggtatctc ttctccgcag gcgtcaatcg atcgagcctc acgcgaaaga agctctcgtc
     2881 gagtgcagcg atcgcggagt gcgatgctgc tttcgacggg ttttatagcc atcttagccg
     2941 tattagccgc ggcgactaat tctcctaagt tccgcctaac cgctggccgc ttgtaccggg
     3001 agtggccgcg ggacacagtc acggcgggca gcgaaaggac acgttcgcac cctggaccgt
     3061 ttcgtgggct cagcttgcca ccatgcgaac cccgccggaa cggtagacgg gccgcccatc
     3121 gcgctggcgc ccgtccatca caaggtccgg cgcgatggct accgacgtgc aagctgaggc
     3181 ggcgcggcgc gcgtctcagc tgtcgaggcg gaaccgtgcg ggcctcccat gtgtcacagt
     3241 ccggcggcat cctgtcctgc gccgggttgc ccccgtggcg aagggaggtt gacgaatgcc
     3301 agacagcttt cacgcactgc tcgtgatgct ggtcgctctg ctgcctgggg cgctttacgt
     3361 c
//
LOCUS       DATMKC010000121        12886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_118626, whole genome shotgun sequence.
ACCESSION   DATMKC010000121 DATMKC010000000
VERSION     DATMKC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118626"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNU01_03580"
     CDS             complement(<1..469)
                     /locus_tag="VNU01_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31733.1"
                     /translation="MEIMMRVAVPDRPGALAALAGAIGEQGGDIQAVEVVDHAQGEAL
                     DDLVVWIDGPERLRALVACVDELDGFRLVHAGPSRGHPGDAVTRVAMGLGALLDTSMT
                     EDHALVTLVGGLLRADSAEMLPAEDAPRADAKTLVLPFADRVLVARRDYRFTET"
     gene            818..2392
                     /locus_tag="VNU01_03585"
     CDS             818..2392
                     /locus_tag="VNU01_03585"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWH31734.1"
                     /translation="MSTDGNDVPAVRIFDTTLRDGEQAPGISLDVKEKLEIAEQLYRL
                     KVDIVEAGFPITSQGDYEAVKAIADTVGTEQGSPVIAALARCHADDIRRAGEALKGAD
                     RKRIHVFLSTSEIHRNAMLHATEDQIVEQTIEGVKRALEYTEDVEFSPQDATRTDYDF
                     LMRVIDAAVEAGATTINVPDTVGYAVPFDFGNLIADVVKRVGEDVFVSVHCHNDLGLA
                     VANSIEAVRNGARQVEVAVNGLGERAGNCSMEEVVMALATRIDLLGVPTNVNTREITR
                     TSRLVSTLTGYPIQFNKAVVGKNAFAHESGIHQHGVLQDRLTYEIMHAEDVGAVGAQI
                     VLGKHSGKHAFSKQLEDMGYTLSKEEVGRAFARFKELADRKVELTDKDLEAIVADEVY
                     ASDEEAYELVGFEVAGGTEHEPEATVKVRVVSDGQVVEEHGSGDGMVDAACSAIRRAT
                     GIDGTLVNFNVSAVTKGVDALGDVTVQVEVEGRRYTGRGMSTDIVEASARAFVNALNK
                     SVRLRNRADVPASETP"
     gene            2407..2637
                     /locus_tag="VNU01_03590"
     CDS             2407..2637
                     /locus_tag="VNU01_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31735.1"
                     /translation="MTEKPPARAGGKPRVRLHVPPDHTPEDEAALVAQVAAAIAESTG
                     PDSADVEVVKTEEVNVTGDVAHRMHQGARWGA"
     gene            2652..4052
                     /gene="leuC"
                     /locus_tag="VNU01_03595"
     CDS             2652..4052
                     /gene="leuC"
                     /locus_tag="VNU01_03595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWH31736.1"
                     /translation="MSGKTLVEKIWERHVVRSAEGEPDLLYIDLHLVHEVTSPQAFEG
                     LRLAGRTVRRPDLTLATMDHNVPTTARELPVTDELSKKQMDTLEANCADFGVRVFNRF
                     SNQVGIVHTIGPELGVTEPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLP
                     QARPKLMAVEISGELPFGVTAKDLILEVIQHIGTDGGVGHVIEYRGPAIEALTMEGRM
                     TVCNMSIEAGARAGLIAPDETTFAWLKGRPHAPQGAAWDEALEYWRTLRTDDDAEFDT
                     VVRLDASTFEPTVTWGTTPSQSVGVSGRVPTPEETRDPEQTRRSLEYMGLRGGEPVTD
                     VKLDRVFIGSCTNGRISDLRAAARVVEGKRVADGVSAMVVPGSYQVKLQAEAEGLDEV
                     FKEAGFDWREPGCSMCLGMNPDILQPGERCASTSNRNFEGRQGKGGRTHLVSPEMAAA
                     AAIHGHFVDVREVIAK"
     gene            4049..4642
                     /gene="leuD"
                     /locus_tag="VNU01_03600"
     CDS             4049..4642
                     /gene="leuD"
                     /locus_tag="VNU01_03600"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWH31737.1"
                     /translation="MNPVTVIEGRMVPLDRADVDTDQIMPKQFLKRIERTGFGEFVFF
                     DWRSDPGFVLNDPRYAGANVMVAGPNFGSGSSREHAPWGLQQYGFDAIVAPSFADIFR
                     NNCAKIGLLAVTLPAETCKRLIAMAEAEPATGIHIDLPNQTLAAGDVDARFDFDPHTK
                     HLLVNGLDDIGMTLQHDDAIAAFEATRPAWLPTTVAR"
     gene            4651..5430
                     /locus_tag="VNU01_03605"
     CDS             4651..5430
                     /locus_tag="VNU01_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH31738.1"
                     /translation="MADPPPAAISTRDLRKVFTGGSGPAVTAVDGVDLDVRPGEVFGL
                     LGPNGAGKTTTIGMLTTRVVPTGGTARIDGIDVAARPTRVKRRIGVVSQTNTLDRSLT
                     VRENLTFHGRYFGLGGRESARRADELLARFRLADRRDADVNTLSGGMAQRLMVARALL
                     HRPAVLFLDEPSTGLDPQSRLALWEVLRGLHAEGQTILLTTHSMDEADQLCERVAIMD
                     HGRILALDTPTELKKAHVEGAPGEATLEDVFIALTGRDLRE"
     gene            5505..6284
                     /locus_tag="VNU01_03610"
     CDS             5505..6284
                     /locus_tag="VNU01_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH31739.1"
                     /translation="MRGFGGLLLRDLRVQSRQPVRFLVRTLSQPFLLVFVFTYVFPRI
                     GQGLRAPGGGESFTTVLVPGVVAIAVFIKGVQAVALPLVQEFGYTREVEDRVLAPLPV
                     PAIAMAKLTWGAIEGVVAALLVFPVVALVPAEPANLAVQWPQLLAVLALGPFLASAVG
                     LFLGTMVPPTQVPLLFSVVILPITFLGATYYPWAALEPIRWLQIVTLANPLVYIAEGF
                     RMALTPQLPHMTSGLVLAALAAFAGAVGYAGVRGFQHRVLS"
     gene            6295..6906
                     /locus_tag="VNU01_03615"
     CDS             6295..6906
                     /locus_tag="VNU01_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWH31740.1"
                     /translation="MHDWDDAERVRQRLAHRLHDGPIQELTAAQLFLDGLALRMESDG
                     ADPALRQSLERGLEALRNATLGCRRLMDTLRPGLEGEGELAERLHRQVAERWPDAVVA
                     VDVPAGFGTDAPADALRVYRVVEELLDEARERGAVVRRVDVAARLGRAEVSVALDAGG
                     GFDLGSDALSRLAAVLETAGGGARVADGGLVALLEVPLGARVP"
     gene            6938..7459
                     /locus_tag="VNU01_03620"
     CDS             6938..7459
                     /locus_tag="VNU01_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31741.1"
                     /translation="MPTMTTVPDYAWNWLQRSRDVVHEIAARLTGGAPAPGFMEGLRP
                     QFTADQFVRDVVIGVVVDVAFGGRVPTRRPAGATWDRGLTWWAAACAGTTPAEFEQRS
                     RPEPVPQPRLFAEPEPPAPPAPEAGRRTPARSRPLDRARMAQALRALLTLEEDGRVPA
                     AEIRALLREIERG"
     gene            7507..10560
                     /locus_tag="VNU01_03625"
     CDS             7507..10560
                     /locus_tag="VNU01_03625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWH31742.1"
                     /translation="MDGLASRPKGPPLSTLQALFDPSPEWPARGRLLWYGAGDAAELE
                     AAAARLGLPAGEAVKVRVAVPAGAQLEARQAEALAVPVGDALPALLRLPRDSGPLHRP
                     VCDSLRAWSAAARLALAAVAAQRAVPALRVAAQAGGPDDAVHGVWRVEPGEHGGRESL
                     VRLAAALPRAAHALVRDDGALWDPLALLEAFEEAVADHAVRAGGEPPAPGRPRARLLP
                     WTARWAAALADPADPVVPLRDDAAELVAAVAAWGRPEDSAGVLHLRLEAPDAEDGPWL
                     VHLALLGSDGLLRPAARVWEPAEDDPLPAPALHEALLRGVALAARLSPPFEDALRAAA
                     PEAVELGLADAWEFLVEAQPALRDAGAVVEVPLGLAEQAVRLRFVVGDDDGAVTDAGT
                     GAEADDFPVDFTDSQEEFVARWEVDLGGEVLGEEELAALAEHEVPLVRWRDQWVLVDP
                     ERLAALRRLRGPARLGLGEALALALSGGHAAELDLDGVTRQEDTEVVARGRVAHLAER
                     IRDAGEPQALEGEPPGFEGELRPYQRRGVAWLGGMAALGLGGILADDMGLGKTVQLIA
                     HLLQADVRAGRLGRHLIVCPTSVLGNWERELHRFAPALPVARFHGQERPDDLGAFQGV
                     VLTSYGMLRREPDPFAAVPWDIVTVDEAQHVKNPATAGARAVRRLRAAQRVAMTGTPL
                     ENRLADLWALMDLVNPGLLGARSVFDRRYATPIERRADQGAARRLRRLVGPFVLRREK
                     RDPAVIADLPDKIEREVVCGLTTEQAALYEETVTEAFRGGLGDGIARRGRVLALLTAL
                     KQICNHPAQYRKREDEDLHGRSGKLATAREIVREAVGSGEQVLVFTQFTSMGRLLVRQ
                     LSEDLGTWVPFLHGGVRAEERDRMVAAFQGEGDVEPPPVLIVSLRAGGTGLNLTAATQ
                     VLHYDRWWNPAVEDQATDRAHRIGQRHAVEVHKLVTAGTVEERIAAMLARKRALADAV
                     VGAGEAWVTELGDAELKELVALSREPGVVVDADWVDDADDVEVSG"
     gene            10557..11345
                     /locus_tag="VNU01_03630"
     CDS             10557..11345
                     /locus_tag="VNU01_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31743.1"
                     /translation="MSARAGDEWARRWAALLAEAPGDIARRARQGFALVRSGRVNDVR
                     VSTGRLSGSVQGSRATPYVAEVAVPVLSEAEWDRIAGTIASQVHHSARLLAGQAPEGL
                     AAELEAMGVRLFPRREELDVACACGQGLCAHAAAVWYAAGEQLAEDPFVLLALRGRGR
                     QRVLDDVAGRRGSVREGAASGIALEALDVAGWWDARGPLDEVGVRSQASAGAPRTPAP
                     ALRALGDPPGWAGGVSAYDLFRPLVERGAGWAAGLLTEADDTDE"
     gene            11387..>12886
                     /locus_tag="VNU01_03635"
     CDS             11387..>12886
                     /locus_tag="VNU01_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH31744.1"
                     /translation="MIRRRLPPLPGALALVLLFLLLPAGPAAAHPLAHPAGPRVLNVR
                     PAGGDVVARGAVTVAALVVSGQDIAAHTVRVDGVVVESTRGTGSHATVAAVVDLAAGD
                     HIAEVVARDAGGRQATRAWRFTVSDLPVRRLRGTGRVETAVAVSADLFPRQGDAAAAV
                     LARADDYADALAGVPLAQHAGGPLLLSAGDALSAPSAEELRRAVAPGGDVYLLGGPAA
                     LSAKVAGDVAALGFRVRRLSGRTRYATAAAIAAELPPAETAVVVSGASFADALAASSP
                     AAANGWPVLLTLPDALPGETRAALDERGVGQVFVVGGTAAVSDAVLEELRARGGAVER
                     LAGPGRYDTADRVARRFFAAAGVAALASGEHFPDALAGSRHAAAHGVPLLLAAPGRLL
                     PPQRAYVEEHAPDRAVVYGGSAAVAERAVADVRRAALDRGGPELVRAEPAEGRVVSTL
                     DQVVLGFDRELVPEHSIVFAEIGGHEVATSLSIGDFPDTLVVSVAELPSN"
BASE COUNT         1620 a         4685 c         4971 g         1610 t
ORIGIN      
        1 ccgtctcggt gaagcggtag tcgcgccggg ccaccagcac gcggtcggcg aacggcagca
       61 ccagcgtctt ggcgtcggcg cgcggcgcgt cctcggcggg cagcatctcg gccgagtcgg
      121 cgcgcagcag gccgccgacc agcgtcacca gcgcgtggtc ctccgtcatg gacgtgtcca
      181 ggagcgcccc gaggcccatg gcgacgcgcg tcaccgcgtc accgggatgg cctcgcgacg
      241 gcccggcgtg gaccaggcgg aagccgtcga gctcgtccac gcaggccacc agcgcgcgca
      301 gccgctccgg cccgtcgatc cagacgacca ggtcgtccag cgcctcgccc tgggcgtgat
      361 cgacgacctc cacggcctgg atgtcgccgc cctgctcgcc tatggcgccg gccagcgcgg
      421 ccagtgcgcc gggccggtcc ggcacggcga cccgcatcat gatctccacc gggcgagcct
      481 agcccgcccc gccgcaggcg acgaaccgac cgccgcaggc gacgaactga tttccggcgg
      541 gcggcgaacc ggccgtccgg gcaggtgcgc gcagttgacg acggcgcccc ctcgggcgta
      601 caactggcct tcgatgaccg acgcagccgc tcctttcgcc gccgggcgcg ccttccgcgc
      661 cctgtcgctg cgcgccatcg tctcggccct gcttgcgggc ctcctgctcc tcctttgatg
      721 ccgcggggcg gactcacacc ggcactcgaa ccccccgcgg agcgcatcca tcgagagtgc
      781 gtaggctgca cgcatcgcgc gcaaggagag caggcacatg agcaccgacg ggaatgacgt
      841 tccggccgtc cgcatcttcg acacgaccct gcgggacggc gagcaggcgc ccggcatctc
      901 gctggacgtc aaggagaagc tcgagatcgc cgagcagctg taccggctga aggtcgacat
      961 cgtcgaggcg ggcttcccga tcaccagcca gggcgactac gaggccgtga aggccatcgc
     1021 cgacaccgtc ggcacggagc agggctcgcc ggtcatcgcc gcgctggcgc gctgccacgc
     1081 cgacgacatc cgccgtgcgg gcgaggcgct gaagggcgcc gaccgcaagc gcatccacgt
     1141 gttcctgtcc acctcggaga tccaccgcaa cgccatgctg cacgccaccg aggaccagat
     1201 cgtcgagcag acgatcgagg gcgtgaagcg cgcgctggag tacaccgagg acgtggagtt
     1261 ctcgccgcag gacgccacgc ggacggacta cgacttcctc atgcgggtga tcgacgcggc
     1321 ggtggaggcg ggcgccacca ccatcaacgt gccggacacg gtcggctacg ccgtgccgtt
     1381 cgacttcggc aacctgatcg ccgacgtggt caagcgcgtg ggcgaggacg tgttcgtgag
     1441 cgtgcactgc cacaacgact tgggcctggc cgtggccaac tccatcgagg ccgtgcgcaa
     1501 cggcgcccgc caggtggagg tcgcggtcaa cgggctgggc gagcgcgccg gcaactgctc
     1561 catggaggag gtcgtgatgg cgctggccac gcgcatcgac ctcttgggcg tgcccaccaa
     1621 cgtcaacacg cgcgagatca cgcgcacctc gcgcctggtg tcgacgctga ccggctaccc
     1681 gatccagttc aacaaggccg tggtgggcaa gaacgcgttc gcccacgagt ccggcatcca
     1741 ccagcacggc gtgctgcagg accgcctgac ctacgagatc atgcacgccg aggacgtcgg
     1801 cgccgtgggc gcgcagatcg tgctgggcaa gcactccggc aagcacgcgt tctccaagca
     1861 gctggaggac atgggctaca cgctgtccaa ggaggaggtc ggccgcgcct tcgcccgctt
     1921 caaggagctg gcggaccgca aggtggagct gaccgacaag gacctggagg cgatcgtcgc
     1981 cgacgaggtc tacgcctccg acgaggaggc ctacgagctg gtcggcttcg aggtcgcggg
     2041 cggtaccgag cacgagcccg aggcgacggt gaaggtgcgc gtggtctccg acggccaggt
     2101 ggtcgaggag cacggcagcg gcgacggcat ggtcgacgcg gcctgctcgg ccatccggcg
     2161 cgccaccggg atcgacggca cactggtgaa cttcaacgtg tccgccgtca ccaagggtgt
     2221 cgacgccctg ggcgacgtca cggtccaggt tgaggtggag ggcaggcgct acaccggccg
     2281 cggcatgtcc accgacatcg tcgaggccag cgcccgcgcc ttcgtcaacg cattgaacaa
     2341 gtcggtgcgg ctgcgcaacc gcgcggacgt gccggcctcc gagacgccgt agggatggtg
     2401 gggaccatga ccgagaagcc cccggctcgt gccggcggga agcccagggt caggctgcac
     2461 gtgccgccgg accacacccc ggaggacgag gccgcgctgg tggcgcaggt cgccgccgcc
     2521 atcgcggagt cgaccgggcc ggactcggcc gacgtggaag tggtcaagac cgaagaggtg
     2581 aacgtcacgg gcgacgtggc gcaccgcatg caccagggcg cccgctgggg cgcgtgaagg
     2641 agctgagcag cgtgtctggg aagacccttg tcgagaagat ctgggagcgc cacgtggtgc
     2701 gctcggccga aggcgagcct gaccttctct acatcgacct tcacctggtg cacgaggtga
     2761 ccagcccgca ggccttcgag ggcctgcgcc tggccgggcg cacggtccgc cgcccggatc
     2821 tcacgctggc caccatggac cacaacgtgc cgaccacggc gcgcgagctg ccggtgaccg
     2881 acgagctgtc caagaagcag atggacacct tggaggccaa ctgcgccgac ttcggcgtgc
     2941 gggtgttcaa ccgcttctcc aaccaggtcg gcatcgtgca caccatcggc cccgagctgg
     3001 gcgtcaccga gcccggcatg acgatcgtct gcggggacag ccacaccgcc acgcacggcg
     3061 cgttcggcgc gctggcgttc ggcatcggca ccagcgaggt ggagcacgtc ctggccacgc
     3121 agacgctgcc gcaggccagg ccgaagctga tggccgtgga gatctccggc gagctgccgt
     3181 tcggcgtcac cgccaaggac ctgatcctgg aggtcatcca gcacatcggc accgacggcg
     3241 gcgtggggca cgtgatcgag taccgcggcc cggcgatcga ggcgctgacg atggagggcc
     3301 gcatgacggt ctgcaacatg tccatcgagg cgggcgcgcg cgccggcctg atcgcgccgg
     3361 acgagaccac gttcgcctgg ctcaagggcc gcccccacgc gccgcagggt gccgcgtggg
     3421 acgaggcgct ggagtactgg cgcacgctgc gcaccgacga cgacgccgag ttcgacacgg
     3481 tggtgcgcct ggacgcgtcc acgttcgagc ccacggtcac gtggggcacg acgccctcgc
     3541 agtcggtcgg cgtgagcggc cgcgtgccca cccctgagga gacgcgcgac ccggagcaga
     3601 cccgccgctc gctggagtac atgggcctgc gaggcggcga gccggtcacc gacgtgaagc
     3661 tggaccgggt gttcatcggc tcctgcacca acggccgcat cagcgacctg cgcgccgccg
     3721 ccagggtggt cgagggcaag cgggtggccg acggcgtcag cgccatggtg gtgcccggct
     3781 cctaccaggt gaagctgcag gcggaggccg agggcctcga cgaggtgttc aaggaggcgg
     3841 gcttcgactg gcgcgagccg ggctgctcga tgtgcctggg catgaacccc gacatcctgc
     3901 agccggggga gcgctgcgcg tcgaccagca accgcaactt cgagggccgg cagggcaagg
     3961 gcggccgcac gcacctggtg tcgccggaga tggcggccgc ggccgccatc cacggccact
     4021 tcgtggacgt gagggaggtc atcgccaagt gaaccccgtg accgtgatcg aagggcgcat
     4081 ggtgccgctg gaccgcgccg acgtcgacac cgaccagatc atgccgaagc agttcctcaa
     4141 gcgcatcgag cgcaccggct tcggcgagtt cgtgttcttc gactggcgct ccgacccggg
     4201 cttcgtgctg aacgacccgc gctacgcggg cgccaacgtc atggtggcgg gcccgaactt
     4261 cggctccggc tccagccgcg agcacgcgcc gtggggcctg cagcagtacg gcttcgacgc
     4321 gatcgtcgcg ccgagcttcg ccgacatctt ccgcaacaac tgcgcgaaga tcggcctgct
     4381 cgccgtgacc ctgccggccg agacctgcaa gcggctgatc gcgatggccg aggccgagcc
     4441 ggccaccggc atccacatcg acctgccgaa ccagacgctg gcggccgggg acgtggacgc
     4501 ccgcttcgac ttcgacccgc acaccaagca cctgctggtc aacgggctgg acgacatcgg
     4561 catgacgctg cagcacgacg acgccatcgc ggcgttcgag gccacgcgcc cggcctggct
     4621 gcccacgacg gtcgcgcgct aggagccgcc ttggctgacc cgcccccggc cgcgatctcg
     4681 acgcgggacc tgcgcaaggt cttcacgggc gggagcgggc ctgcggtcac cgcggtggac
     4741 ggcgtggacc tggacgtgcg ccccggggag gtcttcggcc tcctggggcc caacggcgcc
     4801 ggcaagacca cgacgatcgg catgctgacc acgcgcgtgg ttccgaccgg ggggacggcg
     4861 cgcatcgacg ggatcgacgt ggccgcgcgg cccacgcgag tgaagcggcg catcggcgtg
     4921 gtcagccaga ccaacacgct cgaccgcagc ctgacggtgc gcgagaacct gaccttccac
     4981 gggcgctact tcgggctggg cgggcgcgag tcggcccgcc gggcggacga gctgctggcg
     5041 cgcttccgcc tggccgaccg gcgcgacgcc gacgtcaaca cgctgtccgg gggcatggcg
     5101 cagcggctca tggtggcgcg cgccctgctg caccgtcccg ccgtgctgtt cctggacgag
     5161 ccgagcacgg ggctggaccc gcagagccgg ctggcgctgt gggaggtgct gcgcggcctg
     5221 cacgccgagg gccagacgat cctgctcacc acgcactcca tggacgaggc cgaccagctc
     5281 tgcgagcgcg tggcgatcat ggaccacggc cggatcctgg ccctggacac gcccacggag
     5341 ctcaagaagg cgcacgtcga gggcgcgccg ggagaggcca cgctcgagga cgtcttcatc
     5401 gccctcaccg gaagggacct gcgcgagtga ccgccccgcc ttcccaacct ggaacgtttg
     5461 tccggtatct cggacaaacg ttccaacctg gagcgggcgc ggcggtgcgc ggcttcggcg
     5521 ggctgctgct gcgggacctg cgcgtgcagt cgcgccagcc ggtgcgcttc ctggtgcgca
     5581 cgctcagcca gccgttcctg ctcgtgttcg tgttcaccta cgtgttcccg cgcatcggcc
     5641 agggcctgcg cgcgcccggc ggcggcgagt cgttcaccac cgtgctggtc ccgggcgtgg
     5701 tcgccatcgc cgtgttcatc aagggcgtgc aggccgtcgc cctgccgctc gtgcaggagt
     5761 tcggctacac gcgcgaggtc gaggaccgcg tcctggcgcc cctgccggtt cccgcgatcg
     5821 ccatggccaa gctcacgtgg ggcgcgatcg agggcgtcgt cgccgcgctg ctggtgttcc
     5881 ccgtcgtcgc actggtcccg gccgagccgg cgaacctggc ggtgcagtgg ccgcaactgc
     5941 tcgcggtgct ggcgctgggg ccgttcctgg cgtcggcggt ggggctgttc ctcggcacga
     6001 tggtgccgcc gacccaggtg ccgctcctgt tcagcgtcgt catcctcccg atcacattct
     6061 tgggtgcgac ctactacccg tgggcggccc tggagccgat ccgctggctg cagatcgtca
     6121 ccttggccaa cccgctggtc tacattgccg agggcttccg catggcgctc acgccgcagc
     6181 tgccgcacat gacgtccggc ctggtgctcg cggcgctggc agcgttcgcg ggcgcggtcg
     6241 ggtacgccgg ggtgcggggg ttccagcacc gggtgctgag ctagggggac gccgatgcac
     6301 gactgggacg acgcggaacg cgtgcggcag cggctcgcgc accgcctgca cgacgggccg
     6361 atccaggagc tcaccgccgc gcagctgttc ctggacgggc tggcgctgcg catggaatcc
     6421 gatggcgcgg accccgcgct gcgccagtcc ctggagcgcg ggctggaggc gctgcgcaac
     6481 gccacgctgg gctgccgccg cctcatggac acgctgcgcc ccgggctgga gggggaaggg
     6541 gagctggcgg agcggctgca ccggcaggtg gcggagcgct ggccggacgc cgtcgtggcc
     6601 gtcgacgtgc ccgccggctt cggcacggac gcgcccgcgg acgcgctgcg ggtctaccgc
     6661 gtcgtggagg agctgctcga cgaggcgcgc gagcgcggcg cggtggtgcg ccgcgtcgac
     6721 gtggcggcac ggctcggcag ggcggaggtg tccgtggcgc tggacgccgg cggggggttc
     6781 gacctgggat ccgatgcgct gagcaggctg gcggccgtcc tggaaacggc gggcggcggc
     6841 gcgcgcgtcg ccgacggcgg gctggtggcg ctgctggaag tgccccttgg cgcgcgggtt
     6901 ccttgaccgg cgccgccctg gcggggacgc tgcgcggatg ccgaccatga cgaccgtccc
     6961 ggactacgcc tggaactggc tccagcgctc gcgcgacgtg gtgcacgaga tcgcggcccg
     7021 gctcacgggc ggcgcgcccg cgcccgggtt catggaaggg ctgcgcccgc agttcaccgc
     7081 cgaccagttc gtccgcgacg tggtcatcgg cgtggtggtc gacgtcgcgt tcggcggacg
     7141 cgtgcccacc cgccgcccgg ccggggccac ctgggaccgc gggctgacct ggtgggccgc
     7201 ggcctgcgcc ggcaccacgc cggccgagtt cgagcagcgc tcccggcccg agccggtgcc
     7261 ccagcccagg ctgttcgccg agccggagcc accggcgccg ccggcgccgg aggcgggacg
     7321 ccgcacgccc gcgcgatccc ggccgctgga ccgcgcgcgc atggcgcagg ccctgcgcgc
     7381 cctgctcacg ctggaggagg acgggcgcgt gcccgccgcc gagatccgcg cgctgctcag
     7441 ggaaatcgag cgcggctgag cgtggccgcg gccggccggc agacgggcgc ggcctgagcc
     7501 gctagcgtgg acggactcgc cagccgtccg aaaggtccac ccctgtccac cctccaggcg
     7561 ctgttcgacc cgtcccccga gtggcccgcg cggggacgcc tgctgtggta cggcgccggg
     7621 gacgccgccg aactcgaggc tgccgcggcc cggctgggcc tgcccgccgg cgaggccgtc
     7681 aaggtgcgcg tcgcggtgcc cgccggcgcg caactggagg cgcggcaggc ggaggcgctg
     7741 gccgtcccgg tcggcgacgc cctgccggcc ctgctgcggc tgccgcgcga cagcggcccc
     7801 ctgcaccggc cggtgtgcga cagcctgcgc gcgtggagcg ccgcggcgcg cctcgcgctc
     7861 gcggccgtcg cggcgcagcg cgccgtcccg gcgctgcgcg tggcggcgca agccggcggc
     7921 ccggacgacg cggtgcacgg cgtgtggcgc gtggagcccg gcgagcacgg cgggcgggag
     7981 agcctcgtgc gcctcgccgc cgcgctgccc cgggccgccc acgcgctcgt gcgcgacgac
     8041 ggcgcgctgt gggaccccct cgcgctgctg gaggcgttcg aggaggcggt cgccgaccac
     8101 gcggtgcgcg ccggcggcga gccgcccgcg ccggggcggc cgcgggcgcg cctgctgccg
     8161 tggaccgcgc gctgggccgc cgcgctggcc gacccggccg acccggtcgt gccgctgcgc
     8221 gacgacgccg ccgagctggt ggccgccgtg gccgcgtggg gccgcccgga ggattccgcc
     8281 ggcgtgctgc acctgcgcct ggaggcgccc gacgccgagg acggcccctg gctggtgcac
     8341 ctggcgctgc tgggcagcga cgggctgctg cgcccggccg cccgggtctg ggagccggcc
     8401 gaggacgacc cgctgcccgc ccccgcgctg cacgaggcgc tgctgcgcgg cgtggcgctg
     8461 gcggcgcgcc tgtcgccgcc gttcgaggac gcgctgcgcg ccgcggcgcc ggaggcggtg
     8521 gagctgggcc tcgccgacgc ctgggagttc ctggtggagg cgcagcccgc cctgcgcgac
     8581 gccggggcgg tcgtggaggt gccgctgggc ctggcggagc aggccgtgcg cctgcgtttc
     8641 gtggtgggcg acgacgacgg tgccgtgacc gacgccggca ccggcgcgga ggccgacgac
     8701 ttccccgtcg acttcaccga cagccaagag gagttcgtgg cgcgctggga ggtcgacctg
     8761 ggcggggagg tcctgggcga ggaggagctg gccgcgctgg cggagcacga ggtgccgctg
     8821 gtgcgctggc gagaccagtg ggtgctggtg gacccggagc gcctggcggc gctgcggcgc
     8881 ctgcgcggcc ccgcccggct gggcctgggg gaggcgctcg ccctggcgct gtccggcggc
     8941 cacgccgccg agctggacct ggacggcgtc acccgccagg aggacaccga ggtggtcgcg
     9001 cgcgggcgcg tggcgcacct ggccgagcgc atccgcgacg ccggcgagcc gcaggccctg
     9061 gagggcgagc ctcccggttt cgaaggcgag ctgcggccgt accagcgccg cggtgtggcc
     9121 tggctcggcg gcatggccgc gctcgggctc ggcggcatcc tggccgacga catgggcctg
     9181 ggcaagaccg tgcagctcat cgcgcacctg ctccaggccg acgtgcgcgc cggccggctg
     9241 ggacgccacc tgatcgtctg tcccacctcc gtgctgggca actgggagcg cgagctgcac
     9301 cgcttcgcgc cggccttgcc ggtggcgcgc ttccacggcc aggagcgtcc cgacgacctg
     9361 ggcgccttcc aaggcgtggt gctgacgagc tacggcatgc tgcggcgcga gccggacccc
     9421 ttcgcggccg tgccgtggga catcgtcacg gtggacgagg cgcagcacgt caagaatccc
     9481 gcgacggccg gcgcgcgcgc cgtgcgccgg ctgcgggcgg cgcagcgcgt ggccatgacg
     9541 ggcacgccgc tggagaaccg cctcgcggac ctgtgggcgc tcatggacct ggtcaacccg
     9601 ggactgctgg gcgcgcgcag cgtgttcgac cgccgctacg ccacgccgat cgagcgccgc
     9661 gccgaccagg gcgccgcccg gcggctgcgg cggctggtgg gcccgttcgt cctgcgccgc
     9721 gagaagcgcg acccggccgt gatcgccgac ctgccggaca agatcgagcg cgaggtggtc
     9781 tgcggcctga ccaccgagca ggccgcgctg tacgaggaga ccgtcaccga ggcgttccgc
     9841 ggcggcctcg gcgacggcat cgcccggcgt ggccgggtgc tggcgctgct cacggcgctc
     9901 aagcagatct gcaaccaccc ggcgcagtac cgcaagcgcg aggacgagga cctgcacggg
     9961 cgctcgggca agctcgccac cgcgcgggag atcgtgcggg aggccgtggg ctcgggcgag
    10021 caggtgctgg tgttcacgca gttcaccagc atgggccgcc tgctggtgcg ccagctgtcg
    10081 gaggacctgg gcacctgggt gccgttcctg cacggcggcg tgcgcgcgga ggagcgcgac
    10141 cgcatggtcg ccgcgttcca gggcgagggc gacgtcgagc cgcccccggt gctcatcgtc
    10201 agcctgcgcg ccggcggcac cggcctgaac ctgaccgccg ccacccaggt cctgcactac
    10261 gaccgctggt ggaacccggc cgtggaggat caggcgaccg accgcgcgca ccgcatcggg
    10321 cagcgccacg ccgtcgaggt gcacaagctg gtcaccgccg ggaccgtgga ggagcgcatc
    10381 gccgcgatgc tggcgcgcaa gcgcgcgctg gccgacgcgg tcgtgggcgc cggtgaggcc
    10441 tgggtcaccg agctcggcga cgccgagctc aaggagctcg tcgcgctgtc gcgcgagccg
    10501 ggcgtggtgg tcgacgcgga ctgggtcgac gacgccgacg acgtggaggt gagcgggtga
    10561 gcgcccgtgc cggagacgag tgggcgcggc gctgggcggc gctgctggcc gaggcgcccg
    10621 gggacatcgc ccggcgcgcg cggcagggct tcgccctggt gcgcagcggg cgggtgaacg
    10681 acgtgcgcgt gtccaccggg cggctgtccg gcagcgtcca gggcagccgc gcgacgccgt
    10741 acgtggccga ggtggccgtg ccggtgctga gcgaggccga gtgggaccgc atcgccggca
    10801 cgatcgcctc gcaggtgcac cacagcgccc gcctgctggc cgggcaggcc ccggaggggc
    10861 tggccgcgga gctggaggcg atgggcgtgc gcctgttccc gcggcgcgag gagctggacg
    10921 tggcgtgcgc gtgcggccag gggctgtgcg cgcacgcggc ggcggtctgg tacgccgccg
    10981 gtgagcagct cgccgaagac ccgttcgtcc tgctggcgct gcgcggccgc ggccgccagc
    11041 gcgtcctgga cgacgtcgcg ggccggcgcg gcagcgtccg ggagggcgcg gccagcggca
    11101 tcgcgctgga ggcgctcgac gtggccggct ggtgggacgc gcgcggcccg ctggacgagg
    11161 tgggcgtgcg ctcccaggcc tcggcgggcg cgccgcggac gcccgcgccg gcgttgcgcg
    11221 ccctgggtga cccgcccggc tgggccggcg gcgtgagcgc ctacgacctg ttccggccgc
    11281 tggtggagcg gggcgccgga tgggcggcag ggctcttgac ggaagcggac gatacggacg
    11341 agtaggttgg ccggcgcccg accgatcgct gaaaggctcc gcgtccgtga tccgccgccg
    11401 cctgcccccg ctgccggggg cgctcgccct ggtcctgctg ttcctgctgc tgcccgccgg
    11461 cccggccgcc gcccacccgc tggcgcatcc cgccggcccg cgcgtcctca acgtccgtcc
    11521 cgcgggcggc gacgtggtgg cgcggggagc ggtcaccgtg gccgcgctgg tggtgtccgg
    11581 ccaggacatc gccgcgcaca ccgtccgcgt cgacggcgtg gtggtggagt ccacgcgcgg
    11641 gaccggcagc cacgccacgg tcgccgccgt ggtggacctg gccgcgggcg accacatcgc
    11701 cgaagtcgtc gcgcgggacg ccggcgggcg ccaggccacg cgggcctggc gcttcaccgt
    11761 cagcgacctg cccgtgcggc ggctccgcgg gaccggccgc gtggagaccg cggtggcggt
    11821 cagcgccgac ctgttcccgc gccagggcga cgccgccgcg gcggtcctgg ccagggccga
    11881 cgactacgcc gacgcgctgg cgggggtgcc gctcgcccag cacgccggcg gcccgctgct
    11941 gctgtccgcc ggcgacgccc tgagcgcgcc cagcgccgag gagctgcgcc gcgccgtggc
    12001 ccccggcggc gacgtctacc tgctgggtgg cccggcggcg ctgtcggcga aggtcgccgg
    12061 cgacgtggcg gcgctgggct tccgggtgcg ccggctgtcc gggcgcaccc gctacgccac
    12121 ggccgccgcc atcgccgcgg agctgccgcc cgcggagacg gccgtggtcg tctccggcgc
    12181 ctcgttcgcc gacgcgctcg cggcgtccag cccggcggcg gccaacggct ggccggtgct
    12241 gctgacgctg cccgacgcgc tgcccggcga gacccgcgcc gcactggacg agcgcggcgt
    12301 cgggcaggtc ttcgtggttg gcggcacggc cgcggtgtcc gatgcggtgc tggaggagct
    12361 gcgcgcccgc ggcggcgccg tggagcggct cgcggggccc gggcgctacg acaccgccga
    12421 ccgggtcgca cggcgcttct tcgccgcggc cggcgtggcg gcgctggctt ccggggagca
    12481 cttcccggac gcgctggccg gtagccgtca cgcggctgcg cacggcgtgc cgctgctcct
    12541 ggccgctccg ggtcgcctgc tgccgccgca gcgggcctac gtcgaggagc acgccccgga
    12601 ccgcgccgtc gtctacgggg gctccgccgc cgtggcggag cgcgccgtcg cggacgtgcg
    12661 ccgcgcggcg ctggaccgcg gcgggccgga actggtgcgc gccgagccgg cggaggggcg
    12721 cgtcgtgtcg acgctggacc aggtggtgct cggcttcgac cgcgagctgg tgcccgagca
    12781 ctccatcgtg ttcgccgaga tcggcggcca cgaggtcgcc accagcctgt cgatcgggga
    12841 cttccccgac acgctggtcg tgagcgtcgc ggagctgccg tccaac
//
LOCUS       DATMKC010000122        10565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_16128, whole genome shotgun sequence.
ACCESSION   DATMKC010000122 DATMKC010000000
VERSION     DATMKC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16128"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VNU01_03640"
     CDS             complement(<1..988)
                     /locus_tag="VNU01_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWH31745.1"
                     /translation="MTTPALWVVALALWAGCLHPRGGGALAAAGACAVTLALAPALRG
                     RGGPALALAAAGLVLAGAGLAGAREAAAAGAHLARLAPARAFVTVHARALDDARRTAG
                     GSWQLLRVDAVDGRTVRERVLVRWPGSARPPRLGETVEASGTLRPLGRDGFAASVRLR
                     HAVAELRVADHRLLARAPWHWRAAESLRARAARVFEAGLPDGHAALLLGLTLGVPPPA
                     ELAAPFDAAGLTHLLVVSGQHAVLTVLAVSALAARAGCGFRARRIAALAALAWLVLLV
                     RPQPAVLRAAGAVTAAVGAQLLGRPGVALHVLGAVTLALLLADPFLARQTGFL"
     gene            complement(985..1572)
                     /locus_tag="VNU01_03645"
     CDS             complement(985..1572)
                     /locus_tag="VNU01_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HWH31746.1"
                     /translation="MREDGVLGAVRSRLAAPLAPLQGLALLAVTAVGATALLGRLTQA
                     PAEQPPPQVEIRDEPVVVHVAGHVRSPGLYRLPGGSRVADAVAAAGGATEHADLDGLN
                     LARVVEDGEQLLVPAAGAPGSPERAGVRPDGKVDLNRATAAELDALPGIGPVLAARIV
                     AYRERHGGFRTVRDLRRVQGIGEKLYGSLAELVAV"
     gene            complement(1725..4232)
                     /gene="leuS"
                     /locus_tag="VNU01_03650"
     CDS             complement(1725..4232)
                     /gene="leuS"
                     /locus_tag="VNU01_03650"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWH31747.1"
                     /translation="MAGYDPQEIELRWQRAWDEARLFHADDTSAKPPFYALHMFPYPS
                     GDLHMGHAEAFSLADAVARYARLRNHEVMNPIGWDSFGLPAENAAIKRGIDPKGWTYA
                     NIETQAATMRRMGFAWDWSRRLHTSDPEYYRWTQWIFLQLYGAGWAYRKGANVNWCPS
                     CQTVLANEQVINGLCDRCDTPVTKKSLEQWFFKITDFAQRLLDDMSQLEGQWPERVLT
                     LQRNWIGRSEGAEVTFRIAETGEDVVVYTTRPDTLFGATFFVVAPEHPRAAEWAALGG
                     VAERFEEFLEGVKRKSEIERLATGDAPKQGLALGVHAVNPVNGERLPVYAADYVLMDY
                     GTGAIMAVPAHDQRDLDFARAHGLPVRVVVRPVSSSSEAVGHLGQGAGELPDPDTMTE
                     AAAGDGVTVNSGGYDGLPWREAKRRITDDLQDKGLGRHTVNFRLRDWLISRQRYWGAP
                     IPIIHCPSCGEVPVPEDQLPVRLPEPDEMDFAPTGESPLAKHPTWSQVACPACGGDAT
                     RDTDTMDTFVDSSWYYLRYVSPGRDDVAFDRELVDRWLPVRQYTGGIEHAILHLLYSR
                     FFTKALHDLGHLGFTEPFARLKSQGMVLMDGAAMSKSRGNMVQPKAVVDEFGADTLRA
                     TMLFAGPIEDDVDWADVSTSGMYKWLSRLVRLAGEIAEGAGAGDAGDPLPLRKAAHKT
                     IAAVTEDYEQFKYNTAIAKLMTLANETSAALREHGVRGPALREAIEAIAIMLSPIAPH
                     VCEEIWHRLGNDGFVAAASWPDFDPALLVEDTLQVPVQVDGKVRATMTMPAGASQEHA
                     ESVARAEDNVARHLDGRQVVKVIWVPDRLLNLVTKPA"
     gene            4343..5482
                     /locus_tag="VNU01_03655"
     CDS             4343..5482
                     /locus_tag="VNU01_03655"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HWH31748.1"
                     /translation="MLTRRSLIAAAAGAALGLALSAAQPGAAGPLRIDAGAARTMLGA
                     MLGSLITVAAVLFWIRGTFVQLAAAQFSARVVQGHLSDRHERRALPFLVGAFSYAAVV
                     LLGLPGRGAAPATATLGALLVSVAAFLTVVRTIEVGLRMSQPSTLVLALARHAVTVIR
                     ARLPAGDGSDRAADAHGPPGAGAPVCARASGWLTGVDEERLLGALPPGALLELRTAPG
                     RLVSPGQEIARLHGGGDAEAVADALGGALALSERRPADDIDVALRELVDVTLQAMAPG
                     AGDALRGYEAMDALAWVLAELLSRDVPVGRRERDGRVLLRGAEPSHEVRVRAVLEPLA
                     EVVTRWGPPTRALQRALDDAAARLERQGLPARAALLRRTWADVRG"
     gene            complement(5487..6299)
                     /locus_tag="VNU01_03660"
     CDS             complement(5487..6299)
                     /locus_tag="VNU01_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWH31749.1"
                     /translation="MTRLFQVDAFTATPFSGNPAAVVLLERWPEDEWLRRVSAEMNLA
                     ETAFVLPERDGARPLRWFTPTVEVPLCGHATLATAHALWESGLEPDGAPLAFATRHSG
                     QVTARRDGTHIVLDLPAYAVADKQPPDDVVALAGGAVRGAALVDDLAGDRTWLLELES
                     EAAVRAFRADDAALLATGCGLVLTAAAAPGPGGVDVVSRCFFPGAGISEDPVTGAAHC
                     ALAPYWAPRLGRDELLGYQASPRGGFVRMRLAGDRVELRGQAVTVFTGELRG"
     gene            complement(6296..6538)
                     /locus_tag="VNU01_03665"
     CDS             complement(6296..6538)
                     /locus_tag="VNU01_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31750.1"
                     /translation="MFAVHCPAHGTRVLIWSSGVEAVRNTDDGIEVDYRCTCGHRGTW
                     RTGRRRSERARLTIAKDAGAEYGVMRPPLAVSGSPA"
     gene            6690..8162
                     /locus_tag="VNU01_03670"
     CDS             6690..8162
                     /locus_tag="VNU01_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWH31751.1"
                     /translation="MSERELREALGEWAVRDRPVYLALAERLAELAAQGRVPVGARLP
                     AERRLAQALNVSRGTVVAAYERLREQGIVRTRHGSGTVVDDGSNHLAGPRDAHLTAAL
                     ARNTIFAGFLGDAGDVVDLRGAYWVGTEELPRAAFTHPAAELDQLLEDHGYQPAGLPA
                     LRTAIAGRLTETGLATSPEEVLVTTGAQQAISLIAELVLSPGDRALTEELTFPGALDA
                     FISAEARVRTTPLGAKGVDAAALRDRIARIQPRLVYLCPVHNPTGQVLPALARRRVAE
                     AVSGWEGVVVEDLTLAETQVSGTMPPPIASFTDGDPRTTVLTIGSLSKIMWGGLRIGW
                     IRGPEPVIHRLARIKALVDLGTPLLDQAVAARLLGDIGTIRTRRVAGVQERRGVLSDA
                     LRRELPGWAFTEPEAGLCLWVRLPAASSAVEFAALALRHGVAVVPGTVSAPEGGAGDH
                     LRIAFGQRPDVLEEGVRRLAAAWREWSGLGAPRAELGVLV"
     gene            complement(8221..8856)
                     /gene="wrbA"
                     /locus_tag="VNU01_03675"
     CDS             complement(8221..8856)
                     /gene="wrbA"
                     /locus_tag="VNU01_03675"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HWH31752.1"
                     /translation="MAKIGIVYYSMYGSTFELAKELAEGVRRDGGDPQLRRVAELLPP
                     EVIQEQPGLRASIEAQRDVELARVEELPGFDGIVLGSPTRFGNMTAQLRNFLDQTGPL
                     WAKGELSGKAAGFFTGASTIHGGHESTILTMSTFAFHHGMVVVPLGYAIAAQAGGTRT
                     GGGPYGPSHWSPQEGKDGLDDDEIQIARLYGQHFHRISAQLAGELRERKAA"
     gene            complement(8958..9740)
                     /locus_tag="VNU01_03680"
     CDS             complement(8958..9740)
                     /locus_tag="VNU01_03680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWH31753.1"
                     /translation="MSGRREYAFGDDEEAGNRLAALARLYEDSTRDFVLAHAPAEPAL
                     AVDLGCGPGYTTTLLAGVLRPRRTVGVDDSEAFLERARAGAPEGVEFLRHDVREVPLP
                     LPPADVVFARFLLAHLPEPVAVAERWTGLLAPGGVLLLEDTERIESSNATCARYLELV
                     RATMAAEGRDVFPGGRLAGLRGGEGWSVRHSGTVTLRRPARHYAALFAPNLRQWGPRA
                     LESGVAQELDIRRLATGMEGLLTGDGGGTVSAELRQVVLARS"
     gene            complement(9774..>10565)
                     /locus_tag="VNU01_03685"
     CDS             complement(9774..>10565)
                     /locus_tag="VNU01_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HWH31754.1"
                     /translation="EVDPWGFMRVVDVGAAGGIEQLADPSQWREVAQFSSHHVKDPNP
                     PADSTFTAHNPILGPDGRVYFSWYSNGVRVLDLSDGGATVSEQAWFVPLPSDHEGDND
                     TDPRGIREANAGYWGSWPLCHPGTGDLLVFNADSNRGISILRATYDDCREDALPDLTL
                     ASDDIAFEGRGRTVTITATVRNVGAGDAGPVQVRFEGASSGLIGTVTLAAVPAAGSAV
                     AAVEWDTAGVGSEEVVTVTVDPDGLVAESGTAPNQATATWSRRPV"
BASE COUNT         1300 a         3945 c         3993 g         1327 t
ORIGIN      
        1 gcaggaagcc ggtctggcgc gccaggaacg ggtccgccag cagcagcgcc agcgtgaccg
       61 cccccaggac gtgcagcgcc acgccggggc ggcccagcaa ctgcgccccg acggcggccg
      121 tgaccgcgcc ggcggcgcgc agcaccgccg gctgcgggcg caccagcagc acgagccacg
      181 ccagcgccgc cagggccgcg atccgccgcg cccggaagcc gcagccggcg cgcgcggcca
      241 gcgccgacac ggccagcacg gtgagcaccg cgtgctggcc ggacaccacc agcaggtgcg
      301 tgaggccggc ggcgtcgaag ggcgccgcca gctcggccgg tggcggcacc cccagcgtca
      361 ggcccagcag cagcgcggcg tgcccgtccg gcaacccggc ctcgaagacg cgggcggcgc
      421 gcgcccgcag gctctcggcg gcgcgccagt gccacggcgc ccgggccagc agccggtggt
      481 cggcgacgcg cagctcggcc acggcgtggc gcagccggac ggaggccgcg aagccgtcgc
      541 ggcccagcgg ccgcagcgtg ccggaggctt ccaccgtctc gccgagccgc ggtgggcggg
      601 ccgacccggg ccagcggacc aggacgcgct cacgcaccgt gcgcccgtcg acagcgtcca
      661 cgcgcagcag ctgccacgat cccccggcgg tccggcgcgc gtcgtcgagc gcgcgggcgt
      721 gcacggtgac gaacgcccgt gcgggtgcca gtcgcgccag gtgggcgccc gccgcggctg
      781 cctcgcgcgc gccggcgagc ccggccccgg cgagcaccag gccggcggcg gcgagcgcca
      841 gtgccggccc gccacgcccg cgcagggccg gcgcaagcgc cagcgtcacg gcgcaggcgc
      901 cggcggccgc gagcgcaccg ccgccgcgcg ggtgcaggca gccggcccac agggccagcg
      961 ccaccaccca cagcgcgggc gtggtcatac ggcgacgagc tctgcgagcg agccgtacag
     1021 cttctcgccg atgccctgga cccggcgcag gtcccgcacc gtgcggaagc cgccgtggcg
     1081 ctcgcggtag gcgacgatgc gcgcggccag cacggggccg atgccgggca gcgcgtcgag
     1141 ctcggcggcc gtcgcgcggt tgaggtcgac cttgccgtcc gggcgcacgc ccgcgcgctc
     1201 gggggagccc ggcgcccccg cggccggcac cagcagctgc tcgccgtcct cgaccacgcg
     1261 cgcgaggttc agcccatcga ggtcggcgtg ttccgtggct ccgccggccg cggccacggc
     1321 gtcggccacg cgcgagccgc ccggcagccg gtacagcccg gggctgcgga cgtgcccggc
     1381 gacgtggacg accaccggct cgtcacggat ttccacctgg ggcggcggct gttcggcggg
     1441 cgcctgcgtc agccgtccga gcagcgccgt cgcgcccacc gcggtgacgg cgagcagcgc
     1501 cagcccttgc agcggcgcga gcggcgcagc cagccggctg cgcacggcgc cgagcacccc
     1561 gtcttcgcgc atcgcggcaa gctacggccc gccgcgcgcc gggacgcgtc aggggcgccc
     1621 gaccctgtgg acacgctgga gtgtgaaccg ttgccctcgt attcgaggga aacggttcac
     1681 tgtgaaccgc caggggtcag gcggggcggg ccaggcgaaa ggggtcaggc gggcttggtc
     1741 accaggttga gcaggcggtc gggcacccag atgaccttga ccacctgcct gccgtcgaga
     1801 tggcgtgcga cgttgtcctc ggccctggcc accgactcgg cgtgctcctg cgacgccccg
     1861 gcgggcatgg tcatcgtggc gcgaaccttg ccgtccacct gcaccggcac ctgcaaggtg
     1921 tcctccacca gcagcgccgg gtcgaagtcc ggccacgagg ccgccgcgac gaagccgtcg
     1981 ttgcccagcc ggtgccagat ctcctcgcac acgtgcggcg cgatgggcga cagcatgatc
     2041 gcgatggcct cgatggcctc gcgcagcgcc gggccgcgca caccgtgctc gcgcagcgcc
     2101 gccgaggtct cgttcgccag cgtcatcagc ttggcgatcg cggtgttgta cttgaactgc
     2161 tcgtagtcct cggtgaccgc cgcgatggtc ttgtgcgccg ccttgcgcag cggcagcgga
     2221 tcgcccgcgt cgccggcccc agcgccttcc gcgatctcac cggccaggcg caccaggcgc
     2281 gacagccact tgtacatgcc gctggtggac acgtcggccc agtcgacgtc gtcctcgatc
     2341 ggcccggcga acagcatcgt cgcgcgcagc gtgtccgcgc cgaactcgtc caccaccgcc
     2401 ttgggctgca ccatgttccc gcgcgacttc gacatcgccg cgccgtccat cagcaccatg
     2461 ccctgggact tgagccgcgc gaacggctcc gtgaagccca ggtggcccag gtcgtgcagc
     2521 gccttggtga agaagcgcga gtacagaagg tgcaggatcg cgtgctcgat gccaccggtg
     2581 tactgccgca cgggcagcca gcggtccacc agctcacggt cgaacgcgac gtcgtcgcgc
     2641 cccggcgaga cgtagcgcag gtagtaccag gagctgtcga cgaacgtgtc catcgtgtcg
     2701 gtgtcgcgcg tggcgtcgcc gccgcaggcc gggcaggcca cctgggacca ggtggggtgc
     2761 ttggccagcg gcgactcgcc ggtcggcgcg aagtccatct cgtccggctc cggcagccgc
     2821 accggcagct ggtcctccgg caccggcacc tcgccgcagg acgggcagtg gatgatcggg
     2881 atcggcgccc cccagtagcg ctggcgggag atcagccagt cgcgcaggcg gaagttgacc
     2941 gtgtggcggc ccagcccctt gtcctgcagg tcgtcggtga tgcgccgctt ggcctcgcgc
     3001 cacggcaggc cgtcgtagcc gccggagttc accgtgacgc cgtcgcccgc ggcggcctcg
     3061 gtcatcgtgt cggggtcggg cagctcgccg gcaccctgcc caaggtgtcc taccgcttcg
     3121 ctgcttgagg acaccggccg caccacgacg cgcaccggca gcccgtgggc gcgcgcgaag
     3181 tcgaggtcgc gctggtcgtg ggcgggcacc gccatgatcg cgccggtgcc gtagtccatg
     3241 agcacgtagt ccgcggcgta caccggcagc cgctcgccgt tgacggggtt gacggcgtgc
     3301 acgcccagcg ccaggccctg cttgggcgcg tcgccggttg ccagccgctc gatctcgctc
     3361 ttgcgcttga cgccctccag gaactcctcg aaccgttcgg cgacgccgcc cagcgcggcc
     3421 cactccgccg cgcgcgggtg ctcgggcgcc accacgaaga acgtcgcgcc gaacagcgtg
     3481 tccggacgcg tcgtgtagac cacgacgtcc tcgccggtct cggcgatccg gaacgtgacc
     3541 tcggcgccct ccgagcggcc gatccagttg cgctgcagcg tcagcacgcg ctcgggccac
     3601 tggccctcga gctggctcat gtcgtccagc aggcgctgcg cgaagtcggt gatcttgaag
     3661 aaccactgct ccaggctctt cttggtgacc ggggtgtcgc agcggtcgca cagcccgttg
     3721 atgacctgct cgttggcaag cacggtctgg caggacgggc accagttgac gttggcgccc
     3781 ttgcggtagg cccagcccgc gccgtacagc tgcaggaaga tccactgggt ccagcggtag
     3841 tactccgggt cggaggtgtg caggcgccgg gaccagtccc aggcgaagcc catgcggcgc
     3901 atcgtggcgg cctgggtctc gatgttcgcg tacgtccagc ccttggggtc gatgccgcgc
     3961 ttgatcgcgg cgttctccgc gggcaggccg aagctgtccc agccgatggg gttcatgacc
     4021 tcgtggttcc gcagccgcgc gtagcgcgcg acggcgtcgg ccaggctgaa cgcctcggcg
     4081 tggcccatgt gcaggtcgcc cgaggggtac gggaacatgt gcagggcgta gaagggcggc
     4141 ttggccgacg tgtcgtcggc gtggaacagg cgcgcctcgt cccacgcgcg ctgccagcgc
     4201 agctcgatct cctgcgggtc gtacccagcc actgctcgcc actccttgcg ccccggctga
     4261 caccggcgcg ctcaccgtcg gcccgacgcg ggcaagtcta gccagggtgt ggcggggcgc
     4321 ccccgccggg tagcgccacc gcatgctcac ccgacgatca ctcatcgcgg cggcggccgg
     4381 cgccgcgctg ggactggcgc tgagtgccgc gcagcccggc gccgcgggac cgctgcgcat
     4441 cgacgcgggc gcggcgcgga cgatgctcgg cgcgatgctc ggctcgctga tcaccgtggc
     4501 cgccgtgctg ttctggatcc gcggcacgtt cgtgcagctt gccgccgcgc agttctcggc
     4561 gcgcgtggtg cagggccacc tgtcggaccg ccacgagcgg cgcgccctgc cgttcctggt
     4621 gggcgcgttc tcctacgcgg ccgtggtgct gctgggcctg ccggggcgcg gtgcggcgcc
     4681 ggcgaccgcc acgctgggcg ccctgctggt gtccgtcgcg gccttcctga cggtggtgcg
     4741 caccatcgag gtcggcctgc gcatgagcca gccgagcacg ctggtgctgg ccctggcccg
     4801 ccacgccgtg accgtcatcc gcgcgcggct gcccgccggc gacgggagtg accgcgccgc
     4861 ggacgcgcac gggccgcccg gcgcgggcgc gccggtgtgc gcgcgcgcca gcggctggct
     4921 caccggcgtg gacgaggagc ggctgctggg cgcgctgccg cccggcgcgc tgctggagct
     4981 gcgcaccgcg cccggccggc tggtcagccc cgggcaggag atcgcccggc tgcacggcgg
     5041 cggggacgcc gaggcggtcg cggacgcgct cggcggcgcg ctggcgctca gcgagcggcg
     5101 gccggccgac gacatcgacg tcgcgctgcg cgagctcgtg gacgtgacgc tgcaagccat
     5161 ggcgcccggc gccggtgacg cgctgcgcgg ctacgaggcg atggacgcgc tcgcgtgggt
     5221 cctggccgag ctgctgagcc gagacgtgcc ggtggggcgc cgcgagcgcg acgggcgcgt
     5281 gctgctgcgc ggcgcggagc catcgcacga ggtgcgcgtc cgcgcggtgc tggagccgct
     5341 ggccgaggtg gtgacgcgat ggggaccgcc gacgcgggcg ctccagcgcg cgctggacga
     5401 cgcggcggcg cggctggagc ggcagggcct gcccgcgcgc gcggcgctgc tgcgccggac
     5461 gtgggcggac gtgcgcggct gaggagctac ccgcgaagtt cgccggtgaa cacggtgacg
     5521 gcctgaccgc gcagctccac gcggtccccg gccagccgca tgcgcacgaa gccaccgcgc
     5581 ggggaggcct ggtagcccag cagctcgtcg cgtcccagcc gcggcgccca gtacggcgcc
     5641 agggcgcagt gcgcggcgcc cgtgacgggg tcctcgctga tgccggcgcc ggggaagaag
     5701 cagcgcgaca ccacgtcgac gccgccgggc ccgggcgcgg ccgccgccgt caggacgagg
     5761 ccgcagccgg tggccagcag cgcggcgtcg tcggcgcgga acgcgcgcac cgccgcctcg
     5821 gactccagtt ccaggagcca ggtgcggtca ccggccaggt cgtccaccag cgcggcgccg
     5881 cgcacggcgc cccccgcgag cgcgaccacg tcgtcgggcg gctgcttgtc cgccacggcg
     5941 taggcgggca agtcgagcac gatgtgggtg ccgtcgcggc gcgcggtgac ctggccgctg
     6001 tgacgggtgg cgaacgccag cggcgcgccg tccggctcca ggccgctctc ccacagcgcg
     6061 tgcgccgtgg ccagggtggc gtggccgcac agcggcacct ccacggtggg cgtgaaccag
     6121 cgcaacgggc gggcgccgtc gcgctcgggc agcacgaacg cggtctcggc gaggttcatc
     6181 tcggcggaga cccgccgcag ccattcgtcc tccggccacc gctccaggag cacgaccgcg
     6241 gcggggttgc cggagaacgg cgtcgcggtg aacgcgtcga cctggaacag ccgcgtcacg
     6301 ccgggctccc cgagacggcc agcggcgggc gcatgacccc gtactcggcg ccggcgtcct
     6361 tggcgatggt caggcgcgcc cgctccgagc ggcgccggcc ggttcgccag gtgccgcggt
     6421 ggccgcacgt gcagcggtag tccacttcga tgccatcatc ggtgttgcgg acggcctcca
     6481 cgccggagga ccagatcaac accctcgtcc catgtgccgg gcaatgcacc gcgaacatgt
     6541 cgcctccttc ccaccggcca gtcgccgtaa aagtggacca gtccattcca ccgtgccacc
     6601 cgtaaagtgg cctggaccct ttgtaggtcg gtggactagc attccgtcga tccagtcggc
     6661 gccgaagtgg acaggaccgc accatgctcg tgagcgaacg ggaactgcga gaggcactgg
     6721 gcgagtgggc ggtgcgcgac cgccccgtgt acctggcgct ggccgagcgc ctggccgagc
     6781 tggccgcgca gggccgcgtc ccggtgggcg cccggctgcc cgcggagcgg cgcctggccc
     6841 aggcgctcaa cgtcagccgc ggcaccgtgg tcgccgccta cgagcgcctg cgcgagcagg
     6901 gcatcgtgcg cacccgccac ggcagcggca cggtcgtcga cgacggctcc aaccacctgg
     6961 ccgggccgcg cgacgcccac ctcacggcgg cgctggcgcg caacacgatc ttcgccggct
     7021 tcctgggcga cgccggcgac gtcgtcgacc tgcgcggcgc ctactgggtg ggcaccgagg
     7081 agctgccgcg cgccgccttc acgcaccccg ccgccgagct ggaccagctc ctggaggacc
     7141 acggctacca gccggcgggg ctgccggcgc tgcgcacggc gatcgcgggc cgcctgacgg
     7201 agacgggcct ggcgacgtcg ccggaggagg tgctggtgac caccggcgcc cagcaggcga
     7261 tctcgctgat cgcggagctg gtgctgtccc ccggcgaccg cgccctgacc gaggagctca
     7321 cgttccccgg cgcgctggac gcgttcatct ccgccgaggc ccgggtgcgc accacgccgc
     7381 tcggcgccaa gggcgtcgac gccgcggcgc tacgcgaccg catcgcgcgc atccagccgc
     7441 gcctggtgta cctgtgcccg gtgcacaacc ccaccggcca ggtgctgccc gcgctggcgc
     7501 gccggcgggt cgccgaggcg gtgagcggct gggagggcgt cgtggtcgag gacctcacgc
     7561 tggccgagac ccaggtgtcg gggaccatgc cgccgccgat cgcctcgttc accgacggcg
     7621 acccgcgcac gacggtgctc acgatcggct cgctgtccaa gatcatgtgg ggcggcctgc
     7681 gcatcggctg gatccgcggc ccggagccgg tgatccaccg gctggcgcgc atcaaggcgc
     7741 tggtggacct cggcacgccg ctgctggacc aggccgtggc cgcccggctg ctcggtgaca
     7801 tcggcacgat ccgcacccgg cgcgtcgccg gcgtccagga gcgccgcggc gtgctgtccg
     7861 atgcgctgcg ccgggagctg cccggctggg cgttcacgga gccggaggcg ggcctgtgcc
     7921 tgtgggtgcg gctgcccgcc gcgtccagcg ccgtggagtt cgccgcgctg gcgctgcgcc
     7981 acggcgtcgc ggtggtgccc ggcacggtga gcgcgccgga gggcggcgcc ggcgatcatc
     8041 tgcgcatcgc cttcggccag cgccccgacg tcctcgagga gggcgtgcgc cgcctggccg
     8101 cggcatggcg cgagtggagc ggcctcggcg cgccccgggc ggagctcggc gtcctggtct
     8161 gacccggggt gtgtcgactg ggtcaatgtg aggatgactg agtcgacaca ccgcggttgg
     8221 tcaggcggcc ttgcgctccc gcagctcgcc cgcgagctgg gcggagatgc ggtggaagtg
     8281 ctgcccgtac agccgggcga tctggatctc gtcgtcgtcc agcccgtcct tgccctcctg
     8341 ggggctccag tggctgggac cgtacggacc gccgccggtg cgggtgccgc cggcctgcgc
     8401 ggcgatcgcg tagcccagcg gcacgaccac catgccgtgg tggaaggcga acgtgctcat
     8461 ggtgaggatc gtgctctcgt gcccgccgtg gatggtcgac gcgccggtga agaagcccgc
     8521 ggccttgccg gacagctcgc ccttggccca cagcgggccg gtctggtcca ggaagttgcg
     8581 cagctgcgcg gtcatgttgc cgaagcgggt ggggcttccc aggacgatgc cgtcgaagcc
     8641 gggcagctcc tccacgcgcg cgagctccac gtcgcgctgc gcctcgatgg aggcccgcaa
     8701 gccgggctgc tcctggatca cctcgggcgg cagcagctcg gccacgcgcc gcagctgggg
     8761 gtcgccgcca tcgcggcgga cgccctccgc gagctccttg gcgagttcga acgtgctgcc
     8821 gtacatggag tagtagacga tgccgatctt ggccatgaaa cgattcacct cggggggtct
     8881 ggtcaacggg tgcgtgctgt tcgcgtcccc gcccggtgtg cgcctgacac gcccgcggcg
     8941 cggtcttgac ggccgggcta gctgcgcgcg agcacgacct ggcgcagctc cgcggacacg
     9001 gtgccgccgc catcgccggt gagcagcccc tccatgcccg tggccagccg ccggatgtcc
     9061 agctcctgcg caacaccgga ctccagggcg cgcgggcccc actggcgcag gttcggagcg
     9121 aacagcgcgg cgtagtggcg cgccggccgg cgcagcgtca ccgtgccgga atggcggacg
     9181 ctccagccct cgccgccgcg caggcccgcc agcctgccgc cggggaagac gtcgcggccc
     9241 tccgcggcca tggtcgcgcg caccagctcc aggtagcgcg cgcaggtggc gttggaggac
     9301 tcgatgcgct cggtgtcctc cagcagcagc acgccgccgg gcgcgagcag gccggtccag
     9361 cgctcggcca cggcgacggg ctcgggcagg tgggcgagca ggaagcgggc gaacaccacg
     9421 tcggcgggcg gcagcggcag cggcacctcg cgcacgtcgt ggcgcaggaa ctccacgccc
     9481 tcgggcgcgc cggcgcgcgc ccgctccagg aacgcctcgg agtcgtcgac gccgacggtg
     9541 cggcgcgggc gcagcacgcc cgcgagcagg gtggtggtgt agcccggccc gcagcccagg
     9601 tccacggcga gggccggctc ggcgggtgcg tgcgcgagca cgaagtcgcg ggtgctgtcc
     9661 tcgtacaggc gcgcgagggc cgccagccgg ttgccggctt cctcgtcgtc gccgaacgcg
     9721 tactcccgcc tgccgctcac ccggctcccg tccccgtggc cgccggcgcc acgctacacc
     9781 gggcggcggc tccaggtggc ggtcgcctgg ttgggcgccg tgcccgactc cgccacgagg
     9841 ccgtccgggt cgacggtgac ggtcaccacc tcctcggacc cgaccccggc ggtgtcccac
     9901 tcgacggccg cgacggcgga gccggcggcg ggcacagcgg ccagcgtgac cgtgccgatc
     9961 aggccactgc tcgcgccctc gaagcgcacc tggaccggcc ctgcgtcccc ggcgccgacg
    10021 ttgcgcaccg tcgcggtgat cgtgacggtg cggccgcggc cctcgaacgc gatgtcgtcg
    10081 gacgcgagcg tcaggtcggg cagcgcgtcc tcgcggcagt cgtcgtaggt ggcgcgcagg
    10141 atcgagatgc cgcggttgga gtcggcgttg aacacgagca ggtcgccggt gccggggtgg
    10201 cacagcggcc aggagcccca gtagccggcg ttggcctcgc ggatgccgcg cgggtcggtg
    10261 tcgttgtcgc cctcgtggtc gctcggcagc ggcacgaacc aggcctgctc gctcacggtg
    10321 gcgccgccgt cggagaggtc caggacgcgc accccgttgg agtaccacga gaagtacacg
    10381 cgcccgtcgg gacccaggat cgggttgtgc gccgtgaacg tgctgtccgc gggcgggttg
    10441 gggtccttga cgtggtggct ggagaactgc gccacctcgc gccactgcga cgggtcggcg
    10501 agctgctcga tgccgcccgc cgcaccgacg tcgaccacgc gcatgaagcc ccacgggtcc
    10561 acttc
//
LOCUS       DATMKC010000123         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_133124, whole genome shotgun sequence.
ACCESSION   DATMKC010000123 DATMKC010000000
VERSION     DATMKC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133124"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VNU01_03690"
     CDS             <1..954
                     /locus_tag="VNU01_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWH31755.1"
                     /translation="GARINTAHATGGASCLVSTVKKTTGIPVTHYAEVNFVGFLRMVD
                     AVGGVSLYLSEPMVDRYAGVDLPAGCVTLDGRKALGFVRARHVDSDLGRIARQQRFLK
                     ELASEIAQPATLLNLPRLFRTAHAGAAALTADEDLGPVELLKLARGARGMAGGGIASY
                     TVPAAGARRGGAAVLLPDEDAAADLFAAFATGRVLEASGALPDPSDVTVAVRNGAGIA
                     GLAGQVAEELRAQGFRVADVGNADTVDRTVIRHAPEDELAARRVAEAVPGAVLEVRAP
                     GTPLVLVLGPMSPSSEPPASAEPGEQPPAPAVGAGEPPPDC"
     gene            1002..1958
                     /locus_tag="VNU01_03695"
     CDS             1002..1958
                     /locus_tag="VNU01_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWH31756.1"
                     /translation="MDLYSIANMEPYDDLIRSVQPALAFLQSHVGYDCWTVARKVDDE
                     YIVVAARDRAYGIAGGEVLRWSDTLCARMALGLGPRVAPALAVVPAYTRAPFARHTPV
                     GAYLGVPLHGPDGDLIGSLAALHPTEMPNGVREHLPMVEAMAKTIAGSLAAHVVLAER
                     GRVAERAMADASTDPLTGVGNRRAWEWTIAAEEERCARFRRPAAVISLDVDGLKQTND
                     EHGHAAGDDLLRRAAVAISSAVRAHDVVARLGGDEFGVLAIECDGRTARRLAKRIARA
                     LEEEGVRASMGIAGRADGGLAAAVEAADAAMYAAKRHLRATR"
     gene            complement(1972..>2740)
                     /locus_tag="VNU01_03700"
     CDS             complement(1972..>2740)
                     /locus_tag="VNU01_03700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH31757.1"
                     /translation="QGSGVPFASILSASSPADAMRRSSYVDIVSRADRREVEGVAIAQ
                     VAVAAQREELEAREADLQRVLGEQRELLAQVEAIRDSRAAAAAGVSQQLRKLQAEERH
                     LASESQRIAALSRRSSARVSRSVELPAPAASKAGWTWPVRGPVTSEYGRRWGRQHEGI
                     DIGAGTGSPIYAARAGVVTYVGRMSGYGNLTLVDHGDGIVTAYAHQSRFGVTRGERVD
                     VGERIGSVGCSGSCTGPHLHFEVRVNGSPRNPRRYLP"
BASE COUNT          325 a         1030 c         1053 g          332 t
ORIGIN      
        1 ggcgcgcgca tcaacacggc ccacgccacg ggcggcgcct cctgcctggt gagcacggtg
       61 aagaagacca ccggcatccc cgtcacccac tacgccgagg tgaacttcgt cggcttcctg
      121 cgcatggtcg acgccgtcgg cggcgtgtcg ctgtacctgt ccgagccgat ggtggaccgc
      181 tacgcgggcg tggacctgcc cgccggctgc gtcacgctgg acggccgcaa ggcgctgggc
      241 ttcgtgcgcg cccgccacgt cgacagcgac ctggggcgca tcgcgcgcca gcagcgcttc
      301 ctgaaggagc tggcctccga gatcgcccag cccgcgaccc tgctgaacct gccgcggctg
      361 ttccgcaccg cgcacgcggg ggccgccgcg ctcaccgcgg acgaggacct cggcccggtt
      421 gagctgctga agctggcgcg cggcgcgcgc ggcatggcgg gcggcggcat cgcctcctac
      481 acggtgccgg cggccggcgc gcgccgcggc ggcgccgcgg tgctgctgcc ggacgaggac
      541 gcggcggccg acctgttcgc cgcgttcgcc acgggtcggg tgctggaggc cagcggcgcg
      601 ctgccggacc cgtccgacgt caccgtcgcc gtccgcaacg gcgcgggcat cgcgggactg
      661 gccggccagg tggccgagga gctgcgcgcg cagggcttcc gcgtcgccga cgtgggcaac
      721 gccgacacgg tggaccgcac ggtgatccgc cacgctcccg aggacgagct ggcggccagg
      781 cgggttgccg aggcggtgcc cggagcggtc ctggaggtcc gcgcgccggg cacgcccctg
      841 gtgctggtct tggggccgat gtcgccctcg tctgagccgc cggccagcgc ggagccgggc
      901 gagcagccgc ccgcgccggc ggtcggcgcg ggggagccgc cgccggactg ctgaggccgc
      961 ccgcccttaa ggcggcggcg ccacggccgt tgctatcgga tgtggacttg tactcgatcg
     1021 ccaacatgga gccgtacgac gacctgatca ggtcggtcca gccggccctc gccttcctcc
     1081 agtcgcacgt cggctacgac tgctggacgg tggcgcgcaa ggtggacgac gagtacatcg
     1141 tcgtggcggc ccgcgaccgc gcctacggca tcgccggcgg cgaggtgctg cgctggtccg
     1201 acacgctgtg cgcccgcatg gcgctggggc tggggccccg cgtcgccccg gcgctggcgg
     1261 tggtgcccgc gtacacccgc gcgccgttcg cccgccacac ccccgtcggc gcctacctgg
     1321 gcgtgcccct gcacggtccg gacggcgacc tgatcggctc gctggcggcg ctgcacccca
     1381 cggagatgcc caacggggtg cgcgagcacc tgccgatggt ggaggccatg gccaagacca
     1441 tcgcgggcag cctcgcggcg cacgtggtgc tggccgagcg cggccgcgtg gccgaacgtg
     1501 cgatggccga cgcctccacc gacccgctca ccggcgtggg caaccgccgc gcgtgggagt
     1561 ggacgatcgc cgcggaggag gagcgctgcg cgcggttccg ccgtccggcg gcggtgatca
     1621 gcctggacgt ggacgggctg aagcagacca acgacgagca cggccacgcg gccggcgacg
     1681 acctgctgcg ccgggcggcc gtggcgatct ccagcgccgt gcgcgcgcac gacgtcgtgg
     1741 cgcgcctggg cggcgacgag ttcggggtcc tggcgatcga gtgcgacggc cggacggccc
     1801 ggcgcctggc caagcgcatc gcccgcgccc tggaggagga gggcgtgcgc gcgtcgatgg
     1861 gcatcgcggg ccgcgccgac ggcggcctgg ccgctgcggt cgaagccgcc gacgccgcca
     1921 tgtacgccgc gaagcgccac ctccgcgcca cccgctgacg cggttcgcgg cctacggcag
     1981 gtagcgccgc gggttgcgcg gagagccgtt cacgcgcacc tcgaagtgca ggtgcggccc
     2041 ggtgcagctg ccggagcagc cgacggagcc gatgcgctcg ccgacgtcga cacgttcgcc
     2101 gcgtgtcacg ccgaagcggc tctgatgcgc gtaggccgtg acgatgccgt cgccgtggtc
     2161 gaccagggtg aggttgccgt agccggacat ccgcccgacg taggtgacca cgccggcgcg
     2221 cgccgcgtag atgggcgacc cggtccccgc gccgatgtcg atgccttcgt gctggcggcc
     2281 ccagcggcgt ccgtactccg acgtcaccgg cccgcgcacg ggccacgtcc agccggcctt
     2341 ggaggccgcg ggcgccggca gctccaccga gcgcgacacg cgtgccgagg agcgccgcga
     2401 cagcgccgcg atgcgctggc tctccgaggc gaggtggcgc tcctccgcct ggagcttgcg
     2461 cagctgctgg gacacgccgg cggcggccgc ggcgcggctg tcgcggatgg cctccacctg
     2521 cgccaagagc tcccgctgct cgccgagcac gcgctgcagg tccgcctcgc gcgcctcgag
     2581 ctcctcgcgc tgcgcggcca cggcgacctg cgcgatggcc acgccttcga cctcgcggcg
     2641 atcggcccgg ctgacgatgt ccacgtagga gctgcggcgc atcgcgtcgg cggggctgga
     2701 cgcggacagg atcgaggcga acggcacgcc ggagccctgc
//
LOCUS       DATMKC010000124         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_223041, whole genome shotgun sequence.
ACCESSION   DATMKC010000124 DATMKC010000000
VERSION     DATMKC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223041"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VNU01_03705"
     CDS             <1..223
                     /locus_tag="VNU01_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31758.1"
                     /translation="TSDDFWRYVVAEGRASLGPVATEPGDETCRRLLELYEHVRGAHP
                     NPAEFHDAMVAERRLELSFAPEHWYPVAG"
     gene            456..899
                     /gene="rimP"
                     /locus_tag="VNU01_03710"
     CDS             456..899
                     /gene="rimP"
                     /locus_tag="VNU01_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWH31759.1"
                     /translation="MSDSERVVRGFAEELAAASGLDLVDVALKGQGSRQLIRVMIDRK
                     GGVDLAACQELSRRLSARLDEVDPVPGRYTLEVTSPGVTHPLEGRRAFERVEGRQVRV
                     LRDEAPELHGTVRAADDEAVVLDVEGEQVRVPYADIVKATQALPW"
     gene            946..2094
                     /gene="nusA"
                     /locus_tag="VNU01_03715"
     CDS             946..2094
                     /gene="nusA"
                     /locus_tag="VNU01_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWH31760.1"
                     /translation="MKVEIEALRSIEREKGIAFETVIEALESALASAYKRSRAEAEEA
                     RVVIDRDTGEVVVYAQELDDDGNIVREWVDTPHDFGRIVAQTAKQVLLQRLREAEREI
                     TYGEYSGKEGDLVTGTIQIHDNRVVVLDLGNAEAVLPYAEQVPTERYDHGTRLKAYIT
                     EVRRSIKGPQIICSRTHPGLVEELFKLEVPEIDDGLVRIRGIAREAGHRTKIAVESTN
                     PSIDPVGACVGTRGTRVRAIVEELNEEKIDIITWSDEPAQLVANALSPAKVSAVYLYE
                     EDSTALVVVPDYQLSLAIGREGQNARLAARLTGWRIDIKSETQFAEEQAAFEAAYARG
                     EVDEYGNPIAPPAEGTGPTARPAGEDVQPPAGVGTDSEPESSPAATEG"
     gene            2096..2419
                     /locus_tag="VNU01_03720"
     CDS             2096..2419
                     /locus_tag="VNU01_03720"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HWH31761.1"
                     /translation="MVRTPIRRCLSCRHPQPKTDLVRLVVAENTVVVDPEARRHGRGA
                     YVCARPECLDAALKRGGAALARALRRPGVEVPQQAVRAGWRNACAPTQIAEGSTAAAP
                     REEYE"
     gene            2416..>3011
                     /locus_tag="VNU01_03725"
     CDS             2416..>3011
                     /locus_tag="VNU01_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HWH31762.1"
                     /translation="MSKVRIYELAKETGLPNKEVIRRLAELGVEAKSHSSTVEDDHAA
                     AFRASLGKQREERRKRQEEQQRKEAEEYDLTVLETPPKDTTARRILPPHLRQRQEAAA
                     AAKAAPPAEAPDEPAQAPPPPPRFRPPVAPFRPVGEPGGVSVGGEHEDQAAAAAPAEQ
                     PAAPAEPQAPAARGDVPPATAADAARAGVVRPAGVRPEP"
BASE COUNT          463 a         1075 c         1135 g          338 t
ORIGIN      
        1 gaccagtgac gacttctggc gctacgtggt cgcggagggc cgggcgtcgc tcggcccggt
       61 ggccacggag ccgggggacg agacctgccg gcggctgctg gagctgtacg agcacgtgcg
      121 cggcgcgcac cccaacccgg ccgagttcca cgacgcgatg gtggcggagc gccgcctgga
      181 gctgtcgttc gcgcccgagc actggtaccc ggtcgcgggc tgaggggccg gcgacggggg
      241 ctccgggttc cccgaggcgt tgccgcccct gtggacggct ggtaggttga ggtcaagatg
      301 cgtccggccg ggatggtcgg cgtgatccgg tgcccccgtt cgcggggccg ccggctcgtc
      361 aacttgaggc ggaagcatcg aaagtgggcg tcccgcccac tttttgtttc gtccgcccaa
      421 cggcgcgggc ccggcaggac cacgaaggag gtggcatgag cgacagcgag cgcgtggtgc
      481 gcggcttcgc ggaagagctc gccgccgcct ccgggctgga cctcgtcgac gtcgcgctga
      541 aggggcaagg ctccaggcag ctcatccgcg tcatgatcga ccgcaagggc ggggtggacc
      601 tggccgcgtg ccaggagctg tcccgccggc tgtcggcacg gctggacgag gtggatcccg
      661 tgccgggccg ctacacgctg gaggtcacgt ccccgggggt cacccacccc ctggaggggc
      721 ggcgcgcctt cgagcgcgtg gagggccggc aggtccgcgt gctgcgcgac gaggcgcccg
      781 agctgcacgg cacggtgcgc gccgcggacg acgaggccgt cgttttggac gtggagggcg
      841 aacaggtccg cgtgccctac gcggacatcg tgaaggcgac gcaggcgctg ccctggtagc
      901 gggagccggg acggcacacg ggtcggcaac gagagaggtc gagggatgaa ggtcgagatc
      961 gaggcgctgc gcagcatcga gcgcgagaag ggcatcgcct tcgagacggt gatcgaggcg
     1021 ctggagagcg cgctggccag cgcctacaag cgcagccgcg ccgaggctga ggaggcccgc
     1081 gtcgtcatcg accgcgacac cggcgaggtc gtcgtctacg cgcaggagct ggacgacgac
     1141 ggcaacatcg tccgcgagtg ggtcgacacg ccgcacgact tcggccgcat cgtcgcccag
     1201 accgccaagc aggtgctgct gcagcgcctg cgcgaggccg agcgcgagat cacctacggc
     1261 gagtactccg gcaaggaggg cgacctcgtc acgggcacca tccagatcca cgacaaccgc
     1321 gtcgtggtcc tggacctggg caacgccgag gcggtcctgc cctacgccga gcaggtcccc
     1381 accgagcgct acgaccacgg cacgcgcctc aaggcctaca tcaccgaggt gcgccgctcc
     1441 atcaagggcc cgcagatcat ctgctcgcgc acgcaccccg gcctggtgga ggagctgttc
     1501 aagctggagg tgccggagat cgacgacggc ctggtgcgca tccgcggcat cgcccgcgag
     1561 gccggccacc gcaccaagat cgccgtggag tccaccaacc cctcgatcga cccggtcggc
     1621 gcctgcgtcg gcacgcgcgg cacgcgcgtg cgcgcgatcg tggaagagct aaacgaggag
     1681 aagatcgaca tcatcacctg gtccgacgag cccgcgcagc tcgtcgccaa cgcgctgtcg
     1741 cccgccaagg tgagcgcggt gtacctgtac gaggaggact ccaccgcgct ggtggtggtg
     1801 cccgactacc agctctcgct ggcgatcggc cgcgagggcc agaacgcgcg cctggccgcg
     1861 cggctgaccg gctggcgcat cgacatcaag agcgagacgc agttcgccga ggagcaggcc
     1921 gccttcgagg ccgcgtacgc gcgcggcgag gtggacgagt acggcaatcc gatcgcgccc
     1981 cctgccgagg gcaccgggcc cacggcacgc cccgccggcg aggacgtgca gccgccggcg
     2041 ggcgtcggga cggactcgga acccgagtcg tcccccgcgg ccaccgaggg gtagcatggt
     2101 gaggacaccg atccgacggt gcctctcgtg ccgacacccc cagcccaaga ccgatctggt
     2161 gcggctggtc gtcgcggaga acaccgtcgt ggtcgacccg gaggcgcgcc ggcacggccg
     2221 aggcgcctac gtgtgcgccc ggccggaatg cctcgacgcc gcgctcaagc gcggaggagc
     2281 ggcactcgcc cgagcgttgc gccgtccggg tgtcgaagtc ccacaacaag cggtgcgcgc
     2341 cgggtggcgc aatgcgtgcg caccgacaca gatcgctgaa ggcagtaccg cggccgcgcc
     2401 gcgtgaggag tacgagtgag caaggtccgg atctacgagc tggccaagga gacgggactt
     2461 cccaacaagg aggtcatccg tcgcctcgcc gagctcggcg tggaggcgaa gtcgcactcc
     2521 tccacggtcg aggacgacca cgccgcggcg ttccgcgcca gcctgggcaa gcagcgcgag
     2581 gagcgccgca agcgccagga ggagcagcag cgcaaggagg cggaggagta cgacctcacg
     2641 gtcctggaga cgccgccgaa ggacaccacg gcgcgccgga tcctgccgcc gcacctgcgc
     2701 cagcggcagg aggccgccgc ggcggccaag gccgcgccgc ccgccgaggc accggacgag
     2761 cccgcgcagg cgccgccgcc tccgccgcgc ttccgtccgc cggtcgcccc gttccgcccc
     2821 gtcggcgagc ccgggggtgt cagcgtcggc ggcgagcacg aggaccaggc cgcggccgcc
     2881 gcgccggcag agcagcccgc cgcgcccgcg gagccgcagg cccccgccgc tcgcggggac
     2941 gtgccgccgg cgaccgccgc cgacgccgcg cgcgccggcg tggtgcgccc cgccggcgtg
     3001 cgcccggagc c
//
LOCUS       DATMKC010000125         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_268339, whole genome shotgun sequence.
ACCESSION   DATMKC010000125 DATMKC010000000
VERSION     DATMKC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268339"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VNU01_03730"
     CDS             complement(<1..141)
                     /locus_tag="VNU01_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWH31763.1"
                     /translation="MAKEKFERTKPHLNIGTIGHIDHGKTTLTAAITNVLHKHNPNVA
                     FTP"
     gene            complement(261..333)
                     /locus_tag="VNU01_03735"
     tRNA            complement(261..333)
                     /locus_tag="VNU01_03735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Met,seq:cat)
     gene            complement(353..425)
                     /locus_tag="VNU01_03740"
     tRNA            complement(353..425)
                     /locus_tag="VNU01_03740"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Thr,seq:ggt)
     gene            complement(934..1015)
                     /locus_tag="VNU01_03745"
     tRNA            complement(934..1015)
                     /locus_tag="VNU01_03745"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Tyr,seq:gta)
     gene            1131..1619
                     /locus_tag="VNU01_03750"
     CDS             1131..1619
                     /locus_tag="VNU01_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HWH31764.1"
                     /translation="MADSSFDVVAEVDKQEVDNALNQASKEIATRFDFKNTQAEIAWS
                     GEEIHIQANSEERVLAALDVFKDKLVKRKVSLKSLDAGEPKAGGKGLYHLEIKLVSGI
                     PTDKGKEMIKLIKTSKLKVTPTNMGDHIRVSSKSRDELQEVQALLRANDQDLPLRFTN
                     YK"
     gene            1727..2380
                     /locus_tag="VNU01_03755"
     CDS             1727..2380
                     /locus_tag="VNU01_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31765.1"
                     /translation="MTRRVLTRRVLFVLLPFALLLAGAGLAHAQDASEYASEGASEQA
                     SESASEQASEDTSAQATEDASEEATEGTTEEATSSEEAADGTGTDEDAGNDAADDTEP
                     APEPSGGEDGGDEAAGDDQSDDGSGFTLGLPGGEDAEEAPEPETAEVALETGTAPDAG
                     IAVDPAAGPVPSRVDAGGGYLAASRDQRQGLGWAGLAGLALLALLLPRARAAIRHRG"
     gene            2355..3050
                     /locus_tag="VNU01_03760"
     CDS             2355..3050
                     /locus_tag="VNU01_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWH31766.1"
                     /translation="MPPSAIAADQATPQATAPLWWAVALVVGAAMLGAAATASPSTVP
                     LEIFVEPATPGGAPGGRGAAEPAEPALLVIPSIGVRADVVPVGLHRDGTLETPPATEA
                     GWYRHGTLPGRRGSAVIAGHVDSRSAPGVFAALDRLRPGAVAGVRDTEGVWTLFRVHA
                     LSRHPKDRLPPAVWAPGDRRLLRLITCGGVFDSATGHYRDNVVAHAVALGRWRPPPEQ
                     PAAEPPWPRVAGG"
BASE COUNT          544 a         1195 c         1194 g          456 t
ORIGIN      
        1 cggcgtgaac gccacattcg ggttgtgctt gtgcagcaca ttcgtgatcg ccgcggtcag
       61 cgtcgtcttc ccgtggtcga tatggccgat cgtgccgatg ttcagatgcg gcttcgtccg
      121 ctcgaacttc tccttggcca tgctgtgctc acgtcctccc gggtaggcgg gccgcgcgac
      181 tgcgatcccg tcggttcacg catgcaggac cgagcgatct gcccggtccc gcttgggtgg
      241 tgctgtggtg ttgtgttgcg tagcggcggg tggattcgaa ccaccgacaa ctcgattatg
      301 agccgagtgc tctaacccct gagctacgcc gcctcgtggg tcgtgcgacc ggagcccccc
      361 aacggaatcg aaccgttgac cttctcctta ccatggagac gctctgccga ctgagctagg
      421 ggggcgcaag cgaggcacag tatgaagtgc ccccctgcgg gctgcaacct ggtgcggggg
      481 gcggcctggg gacaacggcc cgccgcggcg cggttttcgt cacacccgcg cgggacgatg
      541 ccgccatgga caccatccgc cgccgcaacc cccgccacca gggcgacatc ggcctgggcg
      601 ccgccatcgc ctggttcagt tcgcagggct accggatcct cgtgcccctg tcggagagcc
      661 aggcctacga cctcgtgatc gacgacggct ccggccccct gcgcgtccag gtcaagacca
      721 cgacgttccg cggccgttac ggccgcttcg tcgtcagcat cgagacagcg ggcggcaatc
      781 agagctttca cacgcgcaag cccttcgaca acaccgccag cgacctgctc ttcgtgcttg
      841 ccgatgatgg gaaccagtac gtcatcccat gcagcgccat acaagcgaag cgcgccctct
      901 ccctgggtga gaagtgggcg cgctttcgcg tcgtggtggg agaaggattc gaaccttcga
      961 aggctgagcc ggcggcttta caggccgctc cctttggcca ctcgggcatc ccaccgtgtg
     1021 acgcgcggaa tggtagggcc gcccgcaaac ccccgcaacc gcgcgcgcta gcatgcccgg
     1081 cgcaactggt gctcccgttg gcgccagacg ccgaggacga ggaggcaagc gtggccgaca
     1141 gcagcttcga cgtggtggcg gaggtcgaca agcaggaggt cgacaacgcg ctcaaccagg
     1201 cgtccaagga gatcgcgacg cgcttcgact tcaagaacac gcaggccgag atcgcctggt
     1261 ccggcgagga gatccacatc caggccaact ccgaggagcg ggtgctggcc gcgttggacg
     1321 tgttcaagga caagctggtc aagcgcaagg tgagcctcaa gtccctggac gccggcgagc
     1381 cgaaggcggg cggcaagggc ctgtaccacc tggagatcaa gctggtctcg ggcatcccca
     1441 cggacaaggg caaggagatg atcaagctga tcaagacctc caagctcaag gtcacgccga
     1501 cgaacatggg cgaccacatc cgcgtcagct ccaagagccg cgacgagctg caggaggtcc
     1561 aggcgctgct gcgcgcgaac gaccaggacc tgcccctgcg cttcacgaat tacaagtaag
     1621 gctaaacgga caattccgca aaggttcggc ctcccgggtt acgcccgcgc ccgcaggtgg
     1681 gaatgcggtt cgacggcaca tgtgccgccg acgacgggga ggtcctatga ccaggcgggt
     1741 tctgaccagg cgagtgctgt tcgtgttgct gcccttcgcg ctgctccttg cgggcgcggg
     1801 gctggcccat gcgcaagacg cgtccgagta cgccagcgaa ggcgcttccg aacaggcgag
     1861 cgagagcgcg tccgagcagg ccagcgagga cacgtccgcg caggccaccg aggacgcctc
     1921 cgaggaggcc acggagggca ccacggagga ggcgacgtcc tccgaggagg ctgcggacgg
     1981 caccggcacc gacgaggacg ccgggaacga cgccgcagac gacaccgagc cggcgcccga
     2041 accatccggc ggtgaggatg gcggcgacga ggcggccggc gacgaccagt ccgacgacgg
     2101 cagcggcttc acgctgggcc tgcccggcgg cgaggacgcc gaggaagcgc cggaaccgga
     2161 gacggccgag gtggcgctgg agaccggtac ggcgcccgac gcgggcatcg ccgtggaccc
     2221 ggcggccggg ccggtgccat cgcgggtcga cgccggcggc ggctacctcg cggcgtcgcg
     2281 cgaccagcgg cagggtctcg gctgggccgg gctggccggc ctcgcgctgt tggcgctgct
     2341 gctgccacgg gcccgtgccg ccatccgcca tcgcggctga ccaggccaca ccccaggcga
     2401 ccgccccgct gtggtgggcg gtcgccttgg tcgtcggcgc cgccatgctc ggcgccgccg
     2461 cgacggcgtc cccgagcacc gtcccactcg agatcttcgt cgagccggcc acgcccggcg
     2521 gcgcgccggg gggccgcggc gcggccgagc cggccgagcc ggcgctgctg gtcatcccgt
     2581 ccatcggcgt gcgcgccgac gtggtgcccg tgggactgca ccgcgacgga acgctcgaga
     2641 cgccgccggc cacggaggcg ggctggtacc ggcacggcac gctgccggga cgccgcggct
     2701 cggcggtgat cgcgggccac gtcgactcgc gctcagcgcc gggcgtcttc gcggcactgg
     2761 accggctgcg ccccggtgcg gtggcgggcg tgcgcgacac cgagggcgtg tggacgctgt
     2821 ttcgcgtgca cgcgctcagc cggcacccga aggaccggct cccgcccgcc gtgtgggcgc
     2881 ccggtgaccg ccgactcctc cggctgatca cgtgtggcgg agtgttcgac tcggccaccg
     2941 gccactaccg cgacaacgtc gtggcgcacg ccgtggcgct ggggcgctgg cgcccgccac
     3001 cggagcagcc cgcggcggag ccgccgtggc cgcgcgtcgc cggcggctga gtcggccgta
     3061 cgccacccga aacggtcgag ccgtcaagac cagcggacgg cttgtcaacc gtaaaaccgc
     3121 aaaaacaccc tcgggaggag tatcagcacg cagcgtgcgg ggcactacgt acgcacggta
     3181 acgcagtggc tccccacggc gcggagccgg acggaaaaga ctcccccaca ggaaggccaa
     3241 gcccatgaag gctttgactc gtgctgccat cgtgctgagt gcagccgcgc tgatggcgct
     3301 gtccctgacc ggcacggcgc tcgccgccca caacgagtgc caccagcccg cgacccccgc
     3361 cacccccgcg acgccggctg ggccggcgt
//
LOCUS       DATMKC010000126         3312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_6501, whole genome shotgun sequence.
ACCESSION   DATMKC010000126 DATMKC010000000
VERSION     DATMKC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6501"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VNU01_03765"
     CDS             <1..232
                     /locus_tag="VNU01_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWH31767.1"
                     /translation="VLRMATSGAADALGFAGRKGIVAPGADADLVAVAGDPVEQLETL
                     LTPFAVLARGRFAVGPAGAAEATEGPLEGPGR"
     gene            complement(236..871)
                     /locus_tag="VNU01_03770"
     CDS             complement(236..871)
                     /locus_tag="VNU01_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31768.1"
                     /translation="MEEELRDRWLRLLAPLGVSEAQAAPVLDDLWRRYGEPGRAYHTI
                     AHVRHVLEVVDFLRGREPVDDPIAIELAVWFHDAVYEPGGQRNEERSAALAAETLLGW
                     GLAAERVAHVVDLVHVTAAHLPGDADGRVLADADLAVLASPPDAYELYRRGVRVEYAH
                     LDDGAWRAGRAAFLRGMLAREAIFSTPTFRGQAERVARANLSAELERLDSG"
     gene            936..1301
                     /locus_tag="VNU01_03775"
     CDS             936..1301
                     /locus_tag="VNU01_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31769.1"
                     /translation="MRSSQHGAPALPVLALLLAVALTSCARGPRAVPLDELAADPDAF
                     HGQTVSLTGHVREIEDDLGDAGRHFVIEDGESNRVRLLPDEAAEPYAGQFVRVVGAFA
                     FDEGTGRRLEVETIERPAP"
     gene            complement(1306..2259)
                     /locus_tag="VNU01_03780"
     CDS             complement(1306..2259)
                     /locus_tag="VNU01_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWH31770.1"
                     /translation="MRLSPAVVSALAVLLAACAPAIGDTVPRDTPAMPVPGKRAVEAA
                     EPAAPSDGVPPAPAEGKPVAPEDAAELARLLTAAESALRDPARTPESLADAGHWQQVA
                     YRKLVQRPELVEPVIAALPAELRAAARGNVLAGERLAAMHSPAKPAQPAETPAAPPTP
                     GPLPKWRILTPPPADQLKAHYQEAEAATGVHWTYLAAVNLVETRMGRIRGDSVVGAQG
                     PMQFMPPTWAEWGEGDVHDPRDAIMAAGRYLRWGGMPGDVDGAIFRYNRDVRYVDAVR
                     AYAEQMQADPRAYYGYYHWQVYYATGGDSVWLREGYDATSG"
     gene            2388..2888
                     /locus_tag="VNU01_03785"
     CDS             2388..2888
                     /locus_tag="VNU01_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWH31771.1"
                     /translation="MSDDLLQNLLAANRDYADGGHDAMAPVTPRLRLAIVTCMDARLM
                     PPDALGLQPGDAHIIRNAGGRVTDDVLRSLAVSCAVLGVRRIVVIPHTQCGMYKSEEE
                     IRAAIRDVSGHDGPARLHEYGDPEAALRRDVERVRSAPFLPPDVSVWGARFDVETGRI
                     TVEVDA"
     gene            2931..3308
                     /locus_tag="VNU01_03790"
     CDS             2931..3308
                     /locus_tag="VNU01_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HWH31772.1"
                     /translation="MPAKEEMPDTIRRSDDKAQRTWSKAHDSAVDTYGEGERAHRTAY
                     SALKHTHEKVGDHWEPKDGKGPSDPGAKGGGPNQSRETFGGVDVEGNTKQELYERAKK
                     LGVDGRSRMDKKELARAIARQQD"
BASE COUNT          436 a         1261 c         1213 g          402 t
ORIGIN      
        1 cgtgctgcgc atggcgacct ccggcgccgc cgacgcgctc ggcttcgccg gtcgcaaggg
       61 gatcgtcgcg cccggcgccg acgcggacct ggtcgcggtc gccggtgatc ccgtcgagca
      121 gttggagacg ctgctcaccc cgttcgcggt cctggcgcgc ggccgcttcg ccgtgggacc
      181 ggccggcgcg gccgaggcga cggagggacc gctggagggc ccaggccgct gacgctcagc
      241 cgctgtccaa gcgctccagt tccgctgaca ggttggcgcg cgccacccgc tccgcctgcc
      301 cccggaacgt gggcgtgctg aagatggcct ccctggccag cattccgcgc aggaacgccg
      361 cgcggccggc gcgccaggcc ccgtcgtcca gatgcgcgta ctccacgcgc acgccccgcc
      421 ggtacagctc gtaggcgtcg ggcggcgagg cgagcaccgc aaggtccgcg tcagcaagca
      481 cccgcccgtc ggcgtcgccg ggcaggtgcg ccgcggtgac gtgcaccagg tcgaccacgt
      541 gcgcgacgcg ctcggccgcc agcccccagc ccagcagcgt ctcggcggcc agggcggcgc
      601 tgcgctcctc gttgcgctgc ccgcccggct cgtacaccgc gtcgtggaac cacacggcaa
      661 gctcgatcgc gatcgggtcg tccacgggct cacggccgcg caggaagtcc accacctcca
      721 gcacatggcg gacgtgcgcg atcgtgtggt acgcccgccc cggctcgccg tagcgccgcc
      781 acaggtcgtc cagcaccggt gccgcctgcg cctcactcac gcccagcggc gcgagcaggc
      841 gcagccagcg gtcgcgcagt tcctcctcca tggcccggca tcctcccacg ccgggcgcgg
      901 cgcgaccggt cggcgggcgg gtagcgtggc gcaccatgcg cagctcacaa catggtgcac
      961 ctgccctgcc cgtcctggcc ctcctgctgg cggtggcctt gacctcgtgc gcgcgaggcc
     1021 cccgcgcggt cccgctggac gagctggccg ccgaccccga cgccttccac ggccagaccg
     1081 tgtccctgac cggccacgtc cgcgagatcg aagacgacct gggcgacgcc gggcgccact
     1141 tcgtgatcga ggatggcgag tccaaccgcg tgcggctgct cccggacgag gccgcggagc
     1201 cctacgcggg ccagttcgtt cgcgtggtcg gcgccttcgc cttcgacgag gggaccggcc
     1261 gccggctgga ggtcgagacc atcgagcgcc cggccccgta aggggtcagc cggacgtggc
     1321 gtcgtagccc tcgcgcagcc agacgctgtc gccgccagtc gcgtagtaga cctgccagtg
     1381 gtagtagccg tagtaggcgc gcgggtccgc ctgcatctgc tcggcgtagg cgcgcaccgc
     1441 gtccacgtag cgcacgtcgc ggttgtagcg gaagatcgcg ccgtcgacgt cgcccggcat
     1501 gccgccccag cgcaggtagc gcccggccgc catgatcgcg tcgcgggggt cgtgcacgtc
     1561 gccctcgccc cactcggccc acgtgggcgg catgaactgc atggggccct gcgcgccgac
     1621 cacgctgtcg ccgcggatgc ggcccatgcg cgtctccacc aggttcacgg cggcgaggta
     1681 cgtccagtgc acaccggtgg ccgcctcggc ctcctggtaa tgggccttga gctggtcggc
     1741 cggcggcggc gtcaggatgc gccacttcgg cagcggcccg ggcgtgggcg gagccgccgg
     1801 cgtctcggcg ggctgggcag gcttggccgg gctgtgcatc gccgcgaggc gctcgcccgc
     1861 gaggacgttg ccgcgcgcgg cggcgcgcaa ctcggccggg agggcggcga tcaccggctc
     1921 caccagctcc gggcgctgca ccagcttgcg gtaggccacc tgctgccagt gcccggcgtc
     1981 ggccaggctc tcgggcgtcc gcgcggggtc gcgcagggcc gactcggccg cggtcagcag
     2041 gcgcgccagc tcggcggcat cctcgggcgc gaccggcttg ccctccgccg gcgccggcgg
     2101 caccccgtcg gacggggccg ccggctccgc ggcctcgacg gcgcgcttgc ccggcacggg
     2161 catggccggg gtgtcgcggg gcaccgtgtc gccgatggcg ggagcgcagg ccgcgagcag
     2221 cacggccaac gcggaaacga ctgcggggga aaggcgcatg cggcaacggt aggcgtgcgg
     2281 cggcatcgtg cagcccgcaa gcgcaaggat cgggccggtg tcgcccccgc gcctgttcct
     2341 ccgggcgggt tttgcgccct ccgcgggaat ccgccatgtt ctggggcatg tccgacgacc
     2401 tgctccagaa cctgctcgcg gccaaccgcg actacgccga cggcggccac gacgccatgg
     2461 cccccgtcac accccgcctg cggctggcca tcgtcacctg catggacgcg cgcctcatgc
     2521 cgccggacgc gctgggcctg cagccgggtg acgcgcacat catccgcaac gccggcgggc
     2581 gggtcaccga cgacgtgctg cgctcgctcg ccgtgtcctg cgccgtgctc ggcgtgcgcc
     2641 gcatcgtggt gatcccgcac acgcagtgcg gcatgtacaa gtcggaggag gagatccgcg
     2701 cggccatccg cgatgtctcc ggccatgacg gcccggcgcg gctccacgag tacggcgacc
     2761 ccgaggcggc gctgcgccgc gacgtcgagc gcgtgcgcag cgcaccgttc ctgcctcccg
     2821 acgtgtccgt ctggggcgcc cgcttcgacg tggagaccgg ccgcatcacc gtggaggtcg
     2881 acgcgtagga cgggccgcgt ccgggcacgc atgcgggtgg aggtgagacg atgcccgcca
     2941 aggaagagat gcccgacacg atccgccgca gcgacgacaa ggcgcagcgc acctggtcca
     3001 aggcgcacga ctcggccgtg gacacctacg gcgagggcga gcgcgcacac cgcaccgcct
     3061 actcggccct gaagcacacg cacgagaagg tcggcgacca ctgggagccc aaggacggca
     3121 aggggcccag cgaccccggc gccaagggcg gcggaccgaa ccagagccgc gagaccttcg
     3181 gcggcgtcga cgtcgagggc aacaccaagc aggagctgta cgagcgcgcc aagaagctcg
     3241 gcgtcgatgg ccgctcccgc atggacaaga aggagctggc ccgggccatc gcccgacagc
     3301 aggactaggg cc
//
LOCUS       DATMKC010000127         4965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_269231, whole genome shotgun sequence.
ACCESSION   DATMKC010000127 DATMKC010000000
VERSION     DATMKC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269231"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VNU01_03795"
     CDS             <1..718
                     /locus_tag="VNU01_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWH31773.1"
                     /translation="NIDPFVEAYVCEHLGLAVEPVATQVVARDRHAELLTAIATLAAS
                     VEQIAVEIRHLQRTEVREAEEPFGAGQKGSSAMPHKRNPITCERITGLARLLRGNAVA
                     ALENVALWHERDISHSSVERVILPDSLIAADYQLHLMIRVIEGLRIFPERMRANLEAT
                     GGLVYSSQVLLSLVDQGMPRDDAYKIVQTAAMRTWETGTPFRETLAQEGVELPESAFD
                     PAGFLARHHVVRERLESLDA"
     gene            complement(715..1629)
                     /gene="ligD"
                     /locus_tag="VNU01_03800"
     CDS             complement(715..1629)
                     /gene="ligD"
                     /locus_tag="VNU01_03800"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWH31774.1"
                     /translation="MSAVTLSSPDKVLWPEAGLTKQDLADYYAAAADRLLPWVRDRPL
                     TLKVFARGVDQDGFFLKNAPKHTPDWIATERVWSPSSNRDIDYLLVRSGEDLRWLAQQ
                     NAVELHPWFSRVDTPDRPDLVAFDLDPADDDPPVWQAARWLKEVLDELGLASIVKTSG
                     KRGLHVYLPVERRYEHPYLRGFALGLSQAVADRHPDRLTTEMRKERRHGRLLMDWSRP
                     GPAQTLVAPWSPRPHPAGTVSMPLAWDEVGPDLDVTAFTLRTALDRPDHWAEPPAPQR
                     IDKARDELCAAGYACEDRNPRGDASRKA"
     gene            complement(1626..1895)
                     /locus_tag="VNU01_03805"
     CDS             complement(1626..1895)
                     /locus_tag="VNU01_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWH31775.1"
                     /translation="MRRVRLIAKGLVQGVWFRASARDRAVALGLTGWVRNRPDGSVEC
                     EAQGDAAAVDAFVEFCRAGPGRAVVERLEVADLEPAEHATGFEVR"
     gene            1934..2113
                     /locus_tag="VNU01_03810"
     CDS             1934..2113
                     /locus_tag="VNU01_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31776.1"
                     /translation="MFAAFLSRRVRRWLLLTVGVPAGAWALQRLGEELEQRRGQSPVA
                     NGLRSAADRLGTLRR"
     gene            2174..3082
                     /locus_tag="VNU01_03815"
     CDS             2174..3082
                     /locus_tag="VNU01_03815"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWH31777.1"
                     /translation="MTDLADELEGAAGVTRLGSGKVRELYEVGSHLLLVASDRVSAFD
                     VILPTPIPGKGEILTRISAFWFARLRNLVPNHLVTMDVEHFPEQLRPSAEALRGRAML
                     CRRAQMLPVECVARGYLAGSGWKEYRDGGTVCGIPLPEGLRESDRLPEPIFTPATKAA
                     VGDHDENIDFARTVEILGAETAERVRELTLALYRAAHEHAAARGIILADTKFEFGWID
                     GELTLCDEALTPDSSRYWPADAYEPGRGQDSYDKQIVRDWLESSGWDKTPPGPALPDD
                     VVARTRARYIEVYERLTGEPFREDVN"
     gene            3082..3324
                     /gene="purS"
                     /locus_tag="VNU01_03820"
     CDS             3082..3324
                     /gene="purS"
                     /locus_tag="VNU01_03820"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWH31778.1"
                     /translation="MPKVHIDVMLKDEILDPQGQAVERQLPGLGVTGVRAVRIGKHIE
                     LDVEDAGDIEARVEDLCAKLLANPVIESWTWRVEQE"
     gene            3366..4088
                     /locus_tag="VNU01_03825"
     CDS             3366..4088
                     /locus_tag="VNU01_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31779.1"
                     /translation="MREHPRDPWRRTRTGEQGDPLLPRWFAILAVVMVPVGLAVAVLA
                     FLNAGRGEVPVAERRPAGEDSLTTAVGGFEAGAAAPRRFEAPCAVVDGMQVAGQERDL
                     RTLESGLSALCERALRAEVVAPLRTFGNFAGVVRFAVFERTGVDSTARAPESGRVPVI
                     YLNAKYSRTDPEWIAPLVAYEAVMLAGEADRAETVLRARRAELAVCRTLGLETRTPGC
                     RDAAALLALPDPAAALGAAGYR"
     gene            4099..4782
                     /gene="purQ"
                     /locus_tag="VNU01_03830"
     CDS             4099..4782
                     /gene="purQ"
                     /locus_tag="VNU01_03830"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWH31780.1"
                     /translation="MRVGVVQFPGSCDERDAAYALTVVGAEPVLLWHGEADLNAVEAV
                     IVPGGFSYGDYLRAGALARFSPVMGAVADFAGRGGPVLGICNGFQVLCEAGLLPGALT
                     RNAGLRFIHREQALCVEATCAWTNGIAPGTVLTVPIKHGEGRYVHDDVDGLAARGQVL
                     VRYCRPDGTVDDAANPNGSLGNIAGVTNEAGNVAGLMPHPEHAVDAFLGGTDGRLIVG
                     SVLQAVAAR"
     gene            4779..>4965
                     /locus_tag="VNU01_03835"
     CDS             4779..>4965
                     /locus_tag="VNU01_03835"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31781.1"
                     /translation="MTDIPKPAPNAPQLAKELGLTHDEYERVVEILGREPSVAELGTF
                     SVMWSEHCSYKSSKIYLK"
BASE COUNT          671 a         1754 c         1866 g          674 t
ORIGIN      
        1 caacatcgac ccgttcgtgg aggcctacgt ctgcgagcac ctgggcctgg ccgtggagcc
       61 ggtcgccacg caggtcgtgg cgcgtgaccg ccacgccgag ctgctcaccg ccatcgcgac
      121 gctggccgcc agcgtcgagc agatcgcggt ggagatccgc cacctgcagc gcaccgaggt
      181 gcgcgaggcg gaggagccgt tcggggccgg gcagaagggc agctcggcga tgccgcacaa
      241 gcgcaacccg atcacctgcg agcgcatcac gggactggcg cggctgctgc gcggcaacgc
      301 ggtcgcggcg ctggagaacg tggcgctgtg gcacgagcgc gacatcagcc acagctccgt
      361 ggagcgggtc atcctgccgg actcgctgat cgccgccgac taccagctgc acctgatgat
      421 ccgcgtcatc gaggggctgc ggatcttccc ggagcgcatg cgcgccaacc tggaggcaac
      481 cggcggcctg gtctacagct cccaggtgct gctgagcctg gtggaccagg ggatgccgcg
      541 cgacgacgcc tacaagatcg tgcagaccgc ggcgatgcgg acgtgggaga ccggcacgcc
      601 gttccgcgag acgctggccc aggagggcgt cgagctgccg gagtcggcgt tcgaccccgc
      661 cggcttcctg gctcgccacc acgtcgtccg cgagcgcctc gagtccctgg acgcctaggc
      721 tttgcggctg gcgtcgcccc gggggttgcg gtcctcgcag gcgtagccgg ccgcgcagag
      781 ctcgtcgcgg gccttgtcga tgcgctgcgg cgcgggcggc tcggcccagt ggtccgggcg
      841 gtccagcgcc gtgcgcaggg tgaacgccgt gacgtcgagg tccggtccga cctcgtccca
      901 ggccaacggc atggagacgg tgccggccgg gtgcgggcgg gggctccacg gcgccaccag
      961 cgtctgggcc ggacccggcc gcgaccagtc catgagcagg cggccgtggc ggcgctcctt
     1021 gcgcatctcg gtggtgagcc ggtccgggtg gcggtcggcg acggcctggc tgagccccag
     1081 ggcgaagccc cgcaggtacg ggtgctcgta gcggcgctcg accggcaggt agacgtgcag
     1141 gccgcgcttg ccgctggtct tcacgatgga ggccagcccc agctcgtcca gcacctcctt
     1201 gagccaccgc gccgcctgcc acacgggcgg gtcgtcgtcg gccgggtcga ggtcgaacgc
     1261 gaccaggtcc ggccggtccg gggtgtcgac gcgggagaac caggggtgga gctcgacggc
     1321 gttctgctgc gcgagccagc gcaggtcctc acccgaccgg acgagcaggt agtcgatgtc
     1381 ccggttcgac gaggggctcc acacccgttc cgtggcgatc cagtccggcg tgtgcttggg
     1441 cgcgttcttc aggaagaagc cgtcctggtc gacgccgcgc gcgaacacct tcagggtgag
     1501 cggccggtcg cgcacccatg gcagcagccg gtccgcggcg gcggcgtagt agtcggcaag
     1561 gtcctgcttg gtcaggccgg cctcgggcca caggaccttg tcgggagacg agagggtgac
     1621 ggcgctcatc ggacctcgaa cccggtcgcg tgctcggcgg gctccaggtc ggccacctcg
     1681 agccgctcga cgaccgctcg cccgggtccg gcccggcaga actcgacgaa cgcgtccacg
     1741 gcggcagcgt ccccctgggc ctcgcattcc acgctgccat cgggccggtt gcgcacccag
     1801 ccggtcaggc cgagcgcgac cgcacggtcc cgcgcggagg cgcggaacca cacgccctgc
     1861 acgaggcctt tggcgatgag acggacgcgc cgcatgggcg tgcttctacc ccgggcgggg
     1921 gagaatggcg cgcatgttcg ccgccttcct gtcccgccgg gtccgccgct ggctcctgct
     1981 cacagtcggt gtgccggccg gggcgtgggc gctgcaacga ctgggggagg agctggagca
     2041 gcgccgcggc cagtcgcccg tggccaacgg cctgcgctcc gccgccgacc gcctcggcac
     2101 cctccgccgc tgacctccca ctgtcccaca ttggaattcg gtgacggcgc ggggcggcgc
     2161 gtagcctgac cgcgtgacgg atctggctga tgaactcgag ggcgcggccg gcgtgacgcg
     2221 cctcggctcg ggcaaagtgc gcgagctgta cgaggtgggc tcgcacctgc tgctggtcgc
     2281 ctccgaccgc gtcagcgcgt tcgacgtgat cctgcccacg ccgatcccgg gcaagggcga
     2341 gatcctcacg cgcatctcgg cgttctggtt cgcccggctt cgcaacctcg tgcccaatca
     2401 cctggtcacc atggacgtcg agcacttccc cgagcagctc cggccctccg ccgaggcgct
     2461 gcgcgggcgg gcgatgctgt gccgccgggc gcagatgctg cccgtcgagt gcgtcgcccg
     2521 cgggtacctg gccggcagcg gctggaagga gtaccgcgac ggcggcacgg tgtgcggcat
     2581 cccgcttccc gagggcctgc gcgagtccga ccgcctgccg gagccgatct tcacgccggc
     2641 caccaaggcc gccgtcggcg atcacgacga gaacatcgac ttcgcacgca ccgtggagat
     2701 cctgggcgcc gagaccgccg agcgtgtccg cgaactgacg ctggcgctgt accgcgccgc
     2761 gcacgagcac gccgccgcgc gcggcatcat cctggccgac acgaagttcg aattcggctg
     2821 gatcgacgga gagctgacgc tgtgcgacga ggcgctgacg ccggacagct cgcgttactg
     2881 gcccgccgac gcgtacgagc ccggccgcgg tcaggacagc tacgacaagc agatcgtgcg
     2941 cgactggctg gaatcgtccg gctgggacaa gacgccgccc gggccggcgc tgcccgacga
     3001 cgtggtggcg cgcacccgcg cgcgctacat cgaggtgtac gagcggctca ccggtgagcc
     3061 cttccgcgag gacgtgaact gatgcccaag gtgcacatcg acgtgatgct gaaggacgag
     3121 atcctggacc cgcagggcca ggccgtggag cgccagcttc ctggtctcgg agtcaccggc
     3181 gtgcgcgcgg tgcgcatcgg caagcacatc gagctggacg tggaggacgc cggcgacatc
     3241 gaagcccgcg tcgaggactt gtgcgccaag ctgctggcca acccggtgat cgagagctgg
     3301 acgtggcggg tggagcagga gtgagcgcac cgccgcccga cggcgcgggg gggaaggcac
     3361 ggggcatgcg tgagcaccct cgcgacccgt ggcgccgcac ccgcaccggc gagcagggcg
     3421 acccgctgct gccgcgctgg ttcgcgatcc tggcggtcgt gatggtcccg gtcgggctcg
     3481 ccgtcgcagt gctcgcgttc ctcaacgccg gacgcggcga ggtgccggtg gccgagcgcc
     3541 ggcccgccgg cgaggacagc ctgaccacgg cggtgggggg cttcgaggcg ggtgcggccg
     3601 cgccgcgccg gttcgaggcg ccgtgcgccg tcgtggacgg catgcaggtc gccggccagg
     3661 agcgcgacct gcggaccctg gagtccgggc tgtcggcgct gtgcgagcgg gcgctgcgcg
     3721 ccgaggtggt cgcgccgctg cgcacgttcg ggaacttcgc gggcgtggtc cggttcgcgg
     3781 tgttcgagcg caccggcgtc gacagcacgg cgcgtgcgcc ggagtcgggc cgcgtccccg
     3841 tcatctacct gaacgccaag tactcgcgca cggaccccga gtggatcgcc cccctcgtcg
     3901 cgtacgaggc ggtcatgctg gccggcgagg cggaccgcgc cgagacggtt ttgcgcgccc
     3961 ggcgggcaga gctggcggtg tgccgtaccc ttggcctgga gacgcggacg cccggctgcc
     4021 gggacgccgc ggccctgctt gccctcccag atcccgcggc cgccttgggg gccgccggct
     4081 accggtagag aaggacacat gcgcgtcggt gtggtgcagt tccccgggtc ctgcgacgaa
     4141 cgggacgcgg cctacgccct gacggtggtg ggcgccgagc cggtcttgct gtggcacggc
     4201 gaggcggacc tcaacgccgt cgaggcggtg atcgtgccgg gcgggttcag ctacggcgac
     4261 tacctgcgtg ccggggcgct ggcgcgtttc tcgccggtga tgggcgcggt cgcggacttc
     4321 gccggccgcg gcgggccggt gctcggcatc tgcaacggct tccaggtctt gtgcgaggcg
     4381 gggctgctgc cgggcgcgct cacgcgcaac gcggggctgc gcttcatcca ccgcgaacag
     4441 gcgctgtgcg tcgaggcgac gtgcgcgtgg accaacggca tcgcgccggg gaccgtgctg
     4501 accgtcccga tcaagcacgg cgagggccgc tacgtgcacg acgacgtcga cgggctcgcc
     4561 gcgcgcggcc aggtgctggt gcgctactgc cggccggacg gcacggtcga cgacgcggcc
     4621 aaccccaacg gcagcctcgg caacatcgcc ggcgtcacca acgaggcggg caacgtcgcg
     4681 gggctgatgc cccatcccga gcacgcggtg gacgcgttcc tcggcggcac cgacgggcgc
     4741 ctgatcgtcg gcagcgtgct ccaggcggtc gcggcgcgat gacggacatt cccaagcccg
     4801 cgccgaacgc tccgcagctc gccaaggagc tcggcctcac ccacgacgag tacgagcgcg
     4861 tggtggagat cctgggccgc gagcccagcg tcgccgagct cggcacgttc agcgtgatgt
     4921 ggtcggagca ctgctcctac aagtcctcca agatctacct gaaga
//
LOCUS       DATMKC010000128         9140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_275335, whole genome shotgun sequence.
ACCESSION   DATMKC010000128 DATMKC010000000
VERSION     DATMKC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275335"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VNU01_03840"
     CDS             complement(<1..677)
                     /locus_tag="VNU01_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH31782.1"
                     /translation="MRALDEELVGELRGLLGDGAVATDPGELASRTRDCWPGLTIRER
                     AGEALPQPGAVVWPADTAQVSALYAWASERGVAVVPYGGGGGVTGGATPLAGCLMVDT
                     KRMTAIGPLDEVSGLVTVGPGVIGQTLEEWLGYRGWTLGHFPSSITVSSVGGFAAARS
                     AGQASTKYGKFEHMVAGLEAVLPDGTVLRRTPQDRAVGQHRLQAGDHVLELAVLGGGL
                     PGRARRG"
     gene            complement(762..3542)
                     /locus_tag="VNU01_03845"
     CDS             complement(762..3542)
                     /locus_tag="VNU01_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWH31783.1"
                     /translation="MSAEPLRPPGAQDDGQAGVEPGELVEAFGGAYGFPLDDFQLLAI
                     TALIEGRSVLVAAPTGAGKTVVGEFAVWQALHRGGKCFYTTPIKALSNQKYADLVRRY
                     GADKVGLLTGDNAINGDAPVVVMTTEVLRNMLYEGSRALVGLHAVVLDEVHYLADRER
                     GAVWEEVIIQLPLSVQLACLSATVSNAEEFGAWLRGVRESCDVVISELRPVPLEHHYF
                     VNGKILPVFRTGSKAAGKKAEAAREERARQARGGVPNPEILMLERRAGVSNRVSRNGR
                     PVASDVRLRPPRRADVVYELRRRTWLPAIYFLFSRQGCDDAVRQLLADRVSLTTPEER
                     RRIREVVAERTAHLPPEDLEVLGHAEWVAGLEQGIAAHHAGMVPVFKETVEELFVANL
                     VKVCFATETLALGINMPARTVVIERLEKWTGQRHELLTPGQFTQLTGRAGRRGLDPIG
                     HAVVLYQRDIDFPTVASLVGRRTEPLRSSFAPSYNMAVNLLRRHTREEAEALLARSFA
                     QYQADATVLGEEERIERNRRALAGYAENLRSDAGDFAEYWALRRELSQLESRTARERK
                     RREADAIERALEELREGDVVAIHGARDSELAAIVGRTQSSKGVPLASAVTIERKLVRL
                     GPREFEAPPAKLGWIRLPKSGGPRSANYRKEIANALRGLKVGPPRSFRKQVRADDEAA
                     RARIEALRTDIRRHPVHSDPALAEIEVWARRHDELRDETERLERGVRRRTGSLVRQFD
                     RILDVLTELGYLEGSEAEPRPTEHGRRLAGIYAETDLVLAESIRRGVLDGLNAADLAA
                     VVSAFAYETRSKDEPVFAFPNEAVRDAVRAAAEVWQDIAAREEQASLPVTRMLDPAFG
                     EVVFRWSQGADLEDALGDAELTPGDFVRSVKQVADLLRQLRDACQGLPLSDVAHQAAK
                     SLVRGVVAYAGV"
     gene            complement(3545..4336)
                     /gene="tatC"
                     /locus_tag="VNU01_03850"
     CDS             complement(3545..4336)
                     /gene="tatC"
                     /locus_tag="VNU01_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWH31784.1"
                     /translation="MDEQSRLDASEMTLVEHLRELRTRLVRSVLAVAAGFAVGFAVHV
                     PVFNLLVKPYCALPSRLRAGSSGLDPDRCVLVFTDVMGGFFLSVKAAAIVAVVLSAPV
                     VCYQIWRFVTPGLRPVERRYALPFIVISQLLFAGGAVFAYFLIPKGLEFLLGFAGENI
                     VSLMDANRYFTFLLQAMIGFGLAFELPLILIMLVLMGVLGSEQLRKARSGALFGIFVA
                     AAVITPTQDPVTMTVMALPLFGFYEVSIVVARLVERRRRRHALAV"
     gene            complement(4424..4633)
                     /locus_tag="VNU01_03855"
     CDS             complement(4424..4633)
                     /locus_tag="VNU01_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWH31785.1"
                     /translation="MSVPGGPEWIVILLIVVLLFGAKKLPELGGSVGKSIKEFKKGVQ
                     DGKNAETDEDDTAAEQHAPTSQTEQ"
     gene            complement(4692..4985)
                     /locus_tag="VNU01_03860"
     CDS             complement(4692..4985)
                     /locus_tag="VNU01_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31786.1"
                     /translation="MPVFVILALALAVMAAAVLALVAVQLWRAVRRLLTASAAVAERL
                     APLTQELQSEAAVTGTESEALQRRLDAWQRARTDRRRAKEDLREGLRRSARGD"
     gene            complement(5022..5366)
                     /locus_tag="VNU01_03865"
     CDS             complement(5022..5366)
                     /locus_tag="VNU01_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31787.1"
                     /translation="MTTIKATCPDCGEVSLTPDEMALHVDSSGGGRSSYAFMCPDCGE
                     DVRKPADERIVRLLLSGGVPVVEEASAPTLADRRFAGPAFTHDDLLDFHTLLESDAWF
                     EVLLEVDRRHVA"
     gene            complement(5507..6442)
                     /locus_tag="VNU01_03870"
     CDS             complement(5507..6442)
                     /locus_tag="VNU01_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWH31788.1"
                     /translation="MSAAPTLQRLERVLLMVPWLLEHPGVTVEEVSERFGVPPADLVR
                     DLDILGYCGLPGYGGGDLVEASIVGDRISVRMADFFSRPLALSLREALTLLLAGSALA
                     AVEDLPESADLASAVAKLRGAMGADARLAIDLRTPGDEHLEALRGAVRDRRVVRLVYR
                     SGSKAETTERLVEPWAVVGARGAWYLHAWCRAAQAPRHFRLDRIRELDVTREPAERRP
                     PETPQPAAYTPEPGDESVVLELEPGATWLAEWVVPDTLEELGGGRLRLAFRTPSVEWV
                     ARVLLRLGADARVVEPAGLRTRAAELAASALRHYA"
     gene            complement(6439..7401)
                     /locus_tag="VNU01_03875"
     CDS             complement(6439..7401)
                     /locus_tag="VNU01_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWH31789.1"
                     /translation="MTDRLERLINLVIALRETRRPMTAEEIRRRVAGYGQDEYEAFRR
                     MFERDKSDLRALGVPIEMAPLDAFETESGYRIDPKRYDLPELHLEPRELTALALALRA
                     TGLYDAAGTGLLKLQVDAGDPDARRVPLEPGLGLDLGAPHLQALMEAQATRTPVRFAY
                     RPLGRDEAQRTVDPHALVHRRGRWYLVGRDHERDARRSFRLDRIAGAVKAAGEPGAFP
                     PPPEVGVDDVVPTAEGGPATAEVLAAPEVAWTVARRARGGGRERPDGWTAFTVAVGEP
                     EDFLAWALDLGPDLVVEEPAELRAEVVARLRAAAAATAAVEGAR"
     gene            complement(7415..8017)
                     /locus_tag="VNU01_03880"
     CDS             complement(7415..8017)
                     /locus_tag="VNU01_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31790.1"
                     /translation="MSALAVRGWIGPGARRLDVAVAAGDCAEAAVPDPRATLRRVAGL
                     AAPRLWVRVGADELGRLPAVARARAGLATVDGALPAARELRVIDAVMAGRPLPGRLGF
                     LGAFTDARAKLAQEREADARALAGVLGLGPLLEDPVAGLDRRAAAVADVARALLAEPR
                     ALVAALPPDEEAAAWLRARLAVEARRRGLPVLLLSRERAG"
     gene            complement(8014..8868)
                     /locus_tag="VNU01_03885"
     CDS             complement(8014..8868)
                     /locus_tag="VNU01_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31791.1"
                     /translation="MRDSPLGLVGAYAAAALVVSAVRPSLAAVLLEITVLALIGLSLD
                     VALRRVGAPALLQPGAVAAGAWATAALLGRNQSPVIALAAAALAGAVAGLAVLGGHPG
                     GPVGLVFAGIAVAILATAIVPPAPGFVPPVFLGVSLASSRATLGVALLALAAGAALYR
                     ATVDGDLGRWLGIARRTPDLVTRVGVSLPAVLGSGLALSGMLAGLAAWAAALGTLGVP
                     AMRPIDPGVGLAWLLIPLAGGGGVAGALGGAAVFAAVTKIAAAAGVPALAIAGPLVAV
                     LWWRGAAR"
     gene            complement(8865..9056)
                     /locus_tag="VNU01_03890"
     CDS             complement(8865..9056)
                     /locus_tag="VNU01_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31792.1"
                     /translation="MAVVALLGRTPLRAAWAGVAAAAVLAVSAALSLDPSAVVAVAVL
                     GLTGLAAARAGRPGQAGAR"
BASE COUNT         1214 a         3558 c         3341 g         1027 t
ORIGIN      
        1 tcgccgcggc gcgctcggcc gggcaggcct ccaccaagta cggcaagttc gagcacatgg
       61 tcgccggcct ggaggcggtg ctgcccgacg gcacggtcct gcggcgtgcg ccgcaggacc
      121 gtgccgtcgg gcagcaccgc ctccaggccg gcgaccatgt gctcgaactt gccgtacttg
      181 gtggaggcct gcccggccga gcgcgccgcg gcgaacccgc cgacgctgga caccgtgatg
      241 gacgacggga agtggcccag ggtccagccg cggtagccca gccactcctc cagggtctgg
      301 ccgatgacgc ctggccccac ggtgaccagg cccgacacct cgtccagcgg gccgatcgcg
      361 gtcatgcgct tggtgtcgac catcaggcag ccggccagcg gcgtcgcgcc gccggtgacg
      421 ccgccgccgc cgccgtaggg caccacggcg acgccgcgct cgctggccca cgcgtacagc
      481 gccgagacct gcgcggtgtc ggccggccac accaccgcgc ccggctgggg cagcgcctcc
      541 ccggcgcgct cgcggatcgt caggccgggc cagcagtcac gggtgcggct ggccagttcg
      601 ccgggatcgg tggccacggc cccgtcgccc agaaggccac ggagctcgcc gaccagctcc
      661 tcgtccagtg cccgcatacc gggcggatcg tacttcccac ccggggtgtg tcgactgagt
      721 cagtcctggg ttgactcagt cgacacaccc cgggtcggcc gttacacgcc cgcgtaggcg
      781 accacgccgc ggaccagcga cttcgcggcc tggtgggcca cgtcggacag cggcaggccc
      841 tggcaggcgt cgcgcagctg gcgcagcagg tccgcgacct gcttcaccga ccgcacgaag
      901 tcgccgggcg tgagctcggc gtcgcccagg gcgtcctcca ggtcggcgcc ctggctccag
      961 cgaaacacca cttcgccgaa cgccgggtcc agcatccgcg tgaccggcaa ggacgcctgc
     1021 tcctcgcgcg cggcgatgtc ctgccacacc tcggcggcgg cgcgcaccgc gtcgcggacc
     1081 gcctcgttgg ggaacgcgaa caccggttcg tccttggagc gcgtctcgta ggcgaaggcg
     1141 ctcacgacgg cggcgaggtc ggcggcgttc agcccgtcga gcaccccgcg ccggatggac
     1201 tcggccagga ccaggtccgt ctcggcgtag atgcccgcca ggcggcggcc gtgctcggtg
     1261 ggccggggct cggcttcgga cccttccagg tagccgagct cggtcagcac gtcgaggatg
     1321 cggtcgaact ggcgcaccag cgaaccggtg cgccgccgca cgccgcgctc cagtcgctcg
     1381 gtctcgtcgc gcagctcgtc gtggcgccgg gcccacacct cgatctcggc cagggccggg
     1441 tcgctgtgca ccgggtggcg gcggatgtcg gtgcgcagcg cctcgatgcg ggcgcgcgcc
     1501 gcctcgtcgt cggccctgac ctgcttgcgg aagctgcgcg gcggcccgac cttcaggccg
     1561 cgcagcgcgt tggcgatctc cttgcggtag ttggccgagc gcgggccgcc ggacttcggc
     1621 aaccggatcc agccgagttt ggccggcggc gcctcgaact cgcgagggcc caggcgcacc
     1681 agcttgcgct cgatggtcac ggcgctggcc agcggcacgc ccttgctgga ctgcgtccgg
     1741 cccacgatcg ccgcgagctc gctgtcgcgc gcgccgtgga tggcgaccac gtcgccctcc
     1801 cgcagctcct ccagcgcgcg ctcgatggcg tccgcctcgc ggcgcttgcg ctcccgcgcg
     1861 gtgcgcgact ccagctgcga cagctcgcgg cgcagcgccc agtactcggc gaagtccccc
     1921 gcgtcggagc gcagattctc cgcgtaaccc gccagcgccc ggcggttgcg ctcgatgcgc
     1981 tcctcctcgc ccagcaccgt cgcgtcggcc tggtactgcg cgaagctgcg cgccagcagc
     2041 gcctcggcct cctcgcgcgt gtgccggcgc agcaggttga ccgccatgtt gtaggacggc
     2101 gcgaagctgg agcgcagcgg ctcggtgcgc cggcccacca gcgaggcgac ggtcgggaag
     2161 tcgatgtcgc gctggtacag caccaccgcg tggccgatcg ggtccaggcc gcggcgccct
     2221 gcccggccgg tgagctgcgt gaactggccg ggtgtgagca gctcgtggcg ctggcccgtc
     2281 cacttctcca gccgctcgat caccacggtg cgcgccggca tgttgatgcc cagcgccagc
     2341 gtctcggtgg cgaagcagac cttcaccagg ttcgcgacga acagctcctc gacggtctcc
     2401 ttgaacaccg gcaccatgcc cgcgtggtgc gcggcgatgc cctgctccag gccggccacc
     2461 cactcggcgt ggcccagcac ctccaagtcc tccggcggca ggtgcgcggt gcgctccgcc
     2521 accacctcgc ggatgcggcg ccgctcctcg ggcgtggtca gcgacacgcg gtcggccagg
     2581 agctggcgca cggcgtcgtc gcagccctgg cgcgagaaca ggaagtagat cgcgggcagc
     2641 cacgtgcgcc ggcgcagctc gtagaccacg tcagcccgcc gcggcgggcg caggcgcacg
     2701 tccgacgcca ccggccgccc gttgcgcgac acgcggttgc tcaccccggc gcgccgttcc
     2761 agcatcagga tctcggggtt gggcacgccg ccgcgcgcct ggcgggcgcg ttcctccctg
     2821 gccgcctcgg ctttcttgcc ggcggccttg gacccggtgc ggaagacggg gaggatcttg
     2881 ccgttgacga agtagtggtg ctccagcggg acgggccgca gctcggagat caccacgtcg
     2941 cagctctcgc gcacgccgcg cagccaggcc ccgaactcct cggcgttgct gaccgtggcg
     3001 gacaggcacg ccagctgcac cgacagcggg agctggatga tgacctcctc ccacacggcg
     3061 ccgcgctcgc ggtcggccag gtagtggacc tcgtccagca ccaccgcgtg cagccccacc
     3121 agcgcgcgcg agccctcgta gagcatgttg cgcaggacct cggtggtcat gaccaccacg
     3181 ggcgcgtcgc cgttgatcgc gttgtccccc gtgagcaggc cgaccttgtc cgcgccgtag
     3241 cggcgcacca ggtcggcgta cttctggttc gacagcgcct tgatcggcgt ggtgtagaag
     3301 cacttgccgc cgcggtgcag cgcctgccac accgcgaact cccccaccac ggtcttgccc
     3361 gcgccggtgg gtgcggccac cagcaccgag cgcccctcga tcagcgccgt gatcgccagg
     3421 agctggaagt cgtccagcgg gaagccgtag gcgccgccga aggcctcgac gagctcgccc
     3481 ggctcgacgc cggcctgacc gtcgtcctgg gcacccgggg gccgcagggg ctcggccgac
     3541 accgctacac cgccagcgcg tgccggcggc ggcggcgctc gaccaggcgg gcgacgacga
     3601 tgctgacctc gtagaagccg aacagcggca gagccatcac ggtcatggtg accgggtcct
     3661 gcgtgggcgt gatcaccgcg gcggccacga agatgccgaa cagcgcgccg ctgcgcgcct
     3721 tgcgcagctg ctcggagccc agcaccccca tgagcacgag catgatcagg atgagcggca
     3781 gttcgaacgc cagcccgaag ccgatcatgg cttgcaggag gaacgtgaag taccggttgg
     3841 cgtccatgag gctgacgatg ttctccccgg cgaagccgag cagaaactcc aggcccttgg
     3901 ggatcaggaa gtacgcgaac acggcgccgc cggcgaacag cagctggctg atcacgatga
     3961 acggcagcgc gtagcggcgc tccacgggcc gcaggcccgg cgtgacgaag cgccagatct
     4021 ggtagcacac gaccggcgcg gacagcacca cggcgacgat ggcggccgcc ttcacgctga
     4081 ggaagaaccc gcccatgacg tcggtgaaca ccaggacgca gcggtcgggg tccaggcctg
     4141 agctgccggc gcgcaaccgg gacggcaggg cgcagtacgg cttgaccagc aggttgaaca
     4201 ccggcacgtg gaccgcgaag ccgaccgcga agccggcggc caccgcgagc acggagcgca
     4261 ccaggcgcgt gcgcagctcg cgcaggtgct ccaccagggt catctcggac gcgtcgaggc
     4321 gcgactgctc atccaccggt ggactggtgg gtggtccggg cgccccgccg gcgcccgatg
     4381 gcgtcgcggt ggctgccatg gggactggac gagcggccgg cgcctactgc tcggtctgcg
     4441 aggtcggggc gtgctgctcg gccgccgtgt cgtcctcgtc cgtctcggcg ttcttgccgt
     4501 cctgcacgcc cttcttgaac tccttgatcg acttgccgac cgagccgccg agctcgggga
     4561 gcttcttggc gccgaacagc agcacgacga tgaggaggat gacgatccac tcggggccac
     4621 cgggcactga catggcgttc ctcctggcgt tgacgggctc tgcgccgagt gtacgcgctc
     4681 gggcccccag gtcagtcccc gcgggcggag cgccgcaggc cttcgcgcag gtcctccttg
     4741 gcgcggcggc ggtcggtgcg cgcccgctgc caggcgtcca ggcggcgctg cagcgcttcg
     4801 ctctcggtcc cggtgacggc ggcctccgac tgcagctcct gggtcagcgg cgccagccgc
     4861 tcggccacgg cggcggaggc ggtgagcagc cggcgcaccg cgcgccacag ctggacggcg
     4921 acgagcgcca ggacggcggc ggccatcacg gccagggcaa gggcgaggat cacgaagacg
     4981 ggcacatccg cagcctagcg aaccggcggc ggaggagccg ctcaggcgac gtggcggcgg
     5041 tcgacctcga gcaggacctc gaaccaggcg tcggactcca gcagcgtgtg gaagtccagc
     5101 aggtcgtcgt gggtgaacgc ggggccggcg aagcgccggt cggccagggt cggcgcggac
     5161 gcctcctcga ccacgggcac gccgccggac agcagcagcc gcacgatgcg ctcgtccgcg
     5221 ggcttgcgca cgtcctcgcc gcagtccggg cacatgaacg cgtagctgga gcggcccccg
     5281 ccggaggagt ccacatgcag cgccatctcg tcgggggtga gcgacacctc gccgcagtca
     5341 ggacaggtcg ccttgatcgt cgtcatggtc ccgcacctcc ctgtaggccg tccggccatc
     5401 ccggctgtca ctcgtccggg tgggggcttc ctgcccctat ggggggatga tcggcctgcg
     5461 ccgcggcggg cttgaggcga aaaaccccag caggcgggcg gggaattcag gcgtagtggc
     5521 gcaaggcgga cgcggccagc tcggccgccc tcgtgcgcag cccggccggc tccaccacgc
     5581 gcgcgtcggc gcccaggcgc agcagcacgc gcgcgaccca ctccacgctc ggcgtgcgga
     5641 acgccagccg caggcggccg ccgccgagct cctccagggt gtccggcacc acccactcgg
     5701 ccagccacgt cgcgccgggc tccagctcca gcaccacgga ctcgtcaccg ggctcggggg
     5761 tgtaggccgc gggctgcggc gtctccggag ggcggcgctc agcaggctcc cgcgtgacgt
     5821 ccagctcccg gatgcggtcc aggcggaagt gccgcggcgc ctgcgccgcg cggcaccacg
     5881 cgtgcaggta ccaggcgccg cgcgcgccca ccaccgccca cggctccacc agccgctccg
     5941 tggtctccgc cttcgacccg gaccggtaca ccagccgcac cacgcggcgg tcgcgcaccg
     6001 cgccgcgcag cgcctccagg tgctcgtcgc ccggcgtgcg caagtcgatt gccaggcgcg
     6061 cgtccgcgcc catcgccccg cgcagcttcg ccacggcgga ggcgaggtcc gccgactcgg
     6121 gcaggtcctc cacggcggcc agggcgctgc cggccagcag cagcgtcagc gcctcgcgca
     6181 ggctgagcgc cagggggcgc gagaagaagt ccgccatgcg caccgagatg cggtcgccca
     6241 cgatggacgc ctcgacgagg tccccgccgc cgtagccggg caggccgcag tagcccagga
     6301 tgtccaggtc gcgcaccagg tcggcgggcg gcacgccgaa gcgctccgag acctcctcga
     6361 cggtcacgcc ggggtgctcc aagagccacg gcaccatcag caggacgcgc tccaggcgct
     6421 gcagggtggg cgccgcgctc accgggcccc ctccaccgcc gccgtggcgg cggccgcggc
     6481 gcgcagccgc gccaccacct cggcgcgcag ctcggcgggc tcctcgacca ccaggtccgg
     6541 tcccaggtcc agggcccagg ccaggaagtc ctccggctcg ccgacggcca cggtgaacgc
     6601 ggtccagccg tcgggccgct cccgcccgcc gccccgggcg cggcgcgcca cggtccaggc
     6661 gacctccggc gcggccagga cctccgccgt cgcgggcccg ccctcggcgg tgggcacgac
     6721 gtcgtcgacg ccgacctccg ggggcggcgg gaacgcgccc ggctcgccgg cggccttcac
     6781 cgcgccggcg atgcggtcca ggcggaacga ccggcgcgca tcccgctcgt ggtcgcggcc
     6841 gaccaggtac cagcggccgc gccggtgcac cagcgcgtgg gggtccacgg tgcgctgcgc
     6901 ctcgtcgcgg cccagcggcc ggtaggcgaa gcgcacgggc gtgcgggtgg cctgtgcctc
     6961 catcagcgcc tgcaggtgcg gcgcgccgag gtcgaggccc agccccggct ccagcggcac
     7021 ccggcgggcg tccgggtcgc cggcgtcgac ctggagcttg agcaggccgg tcccggcggc
     7081 gtcatagagg cccgtggcgc gcaaggccag tgccagggcg gtgagctcgc gcggctccag
     7141 gtgcagctcc ggcaggtcgt agcgcttggg gtcgatgcgg tagccggact cggtctcgaa
     7201 ggcgtccagc ggcgccatct cgatcggcac cccgagcgcg cgcaggtcgg acttgtcccg
     7261 ctcgaacatg cggcggaacg cctcgtactc gtcctgcccg tagccggcga cccgccgccg
     7321 gatctcctcg gcggtcatcg ggcgccgggt ctcgcgcagg gcgatgacca ggttgatcag
     7381 tcgttccagg cggtcggtca tggaggcgct gagcctatcc cgcgcgctcc cgcgacagca
     7441 gcagcaccgg caggccgcgc cggcgcgcct ccacggccag gcgcgcacgc agccacgccg
     7501 cggcctcctc gtccggtggc agcgcggcga cgagcgcacg cggctcggcc agcagcgccc
     7561 tggccacgtc ggccacggcg gcggcgcgcc ggtcaaggcc ggccacgggg tcctccagca
     7621 gcgggccgag ccccagcacg ccggccagcg cccgggcgtc ggcctcccgc tcctgggcga
     7681 gcttcgcgcg cgcgtcggtg aacgccccca ggaagccgag cctgcccggc agcgggcggc
     7741 ccgccatcac cgcgtcgatg acccgcagct cccgcgcggc gggcagcgcc ccgtccaccg
     7801 tggccaggcc cgcgcgcgcc cgcgcgaccg cgggcagccg ccccagctcg tccgcgccca
     7861 cccggaccca cagccgtggc gcggcgagcc ccgcgacgcg ccgcagggtg gcgcgcgggt
     7921 cgggcaccgc ggcctcggcg cagtcccccg ccgccaccgc cacgtccagg cggcgcgcac
     7981 cgggaccgat ccagccgcgc acggccagcg cgctcaccgc gcggctcccc gccaccacag
     8041 gacggcgacg agcggcccgg cgatggccag cgccgggacc ccggccgccg cagcgatctt
     8101 ggtgacggcg gcgaacacgg ccgcgccgcc gagcgcgccc gccacgccgc cgccgcccgc
     8161 gagggggatg agcagccacg ccagtccgac gcccgggtcg atcggccgca tcgcgggcac
     8221 gccgagggtc cccagcgccg cggcccacgc cgccaggccc gccagcatgc cggacaacgc
     8281 gagcccggag cccagcaccg cgggcaggga caccccgacc cgcgtgacca ggtccggcgt
     8341 gcgccgcgcg atgcccagcc agcgccccag gtcgccgtcg acggtggcgc ggtacagggc
     8401 ggcgcccgcg gccagggcca gcagggccac gcccagcgtg gcgcggctcg acgccaggct
     8461 cacaccaagg aagaccggcg gcacgaagcc cggtgcgggc ggcacgatcg cggtggccag
     8521 gatcgccacc gcgatgccgg cgaagaccag cccgaccggc ccgccgggat gcccgcccag
     8581 caccgccagc ccggcgacgg cgcccgccag cgccgcggcg gccagggcga tcacgggtga
     8641 ctggttgcgg cccagcagcg ccgccgtggc ccacgcgccg gcggcgaccg ccccgggctg
     8701 cagcagcgcc ggcgcgccga cgcggcgcag cgccacgtcc agggacaacc cgatcagcgc
     8761 gagcaccgtg atctccagca gcaccgccgc cagggacgga cgcacggcgg acaccacgag
     8821 cgccgcggcg gcgtaggcgc ccaccagccc caggggcgag tccctcaccg ggcgcccgcc
     8881 tggccgggcc tgcccgcgcg agccgccgcc aggcccgtga ggccgagcac ggcgacggcg
     8941 accacggccg acggatcgag cgacagcgcg gcgctgacgg ccaggacggc ggcggcggcc
     9001 acgcccgccc acgcggcgcg caggggcgtc cggccgagca gcgcgacgac ggccaccgtg
     9061 accgccagcg tcagcggctc cgggcgggcg taggcgtgcg cggccgccgc ccagggcgcc
     9121 gcggccgcgc acgcctacgc
//
LOCUS       DATMKC010000129         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_189626, whole genome shotgun sequence.
ACCESSION   DATMKC010000129 DATMKC010000000
VERSION     DATMKC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189626"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VNU01_03895"
     CDS             complement(<1..569)
                     /locus_tag="VNU01_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWH31793.1"
                     /translation="MPVDPEKALGAELPPVTGSWAPDDVILYHLGVGAGDPPTDPREL
                     AYTYEGGLKVLPTFGVIAAAPALAGMLEIPGLEINWATLLHGEQDLTIHRPLPTSADV
                     VTSGRVAGLYDKGKAALLVLDVDTREEGGEALFTNRYSLFLRGEGGFGGEPGPQPGND
                     APERPADFTVVSPTLPQQALLYRLSGDKNP"
     gene            complement(585..1700)
                     /locus_tag="VNU01_03900"
     CDS             complement(585..1700)
                     /locus_tag="VNU01_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31794.1"
                     /translation="MNFAFSDEQLELGRIVRQFLEDKSGEPEVRRLMDTEQGYDPAVW
                     RQMAEQMGLQGLIVPEEHGGAGYSFLELVVVLEQMGAALLCAPYFSTVVLGVSTLLHA
                     GDDAARKEWLPGIAAGDTVATLALTEDNGRWDAEGVQLEVRRDGERWTLHGHKSYVLD
                     GHVADLVLVAARTEGGVTLFAVPGDAPGLARTVLSTMDQTRKLARLEFDAVPARLVGQ
                     EGGGWQVVERVLDLAAVALAAEQVGGAQRCLDLSVAYAKERIQFGRPIGSFQAVKHRC
                     ADMLVAVEQAKSAAYYAGWCAAELDADLPVAAAMAKAYCSEAYFKAAADTIQIHGGIG
                     FTWEHPAHLYFKRAKSSELLFGDPAHHRELLAQRLGI"
     gene            1795..>2513
                     /locus_tag="VNU01_03905"
     CDS             1795..>2513
                     /locus_tag="VNU01_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31795.1"
                     /translation="MADATEQPRERVRDLTRRLVAAHDPATTPAEELWGAQFDAGLAW
                     IHFPEGRGGLGLPPDLQMVVDEELRAAGAPSNVLRNFMGVGMAGPTLVAHGTPEQQER
                     LLRPLFTCEEIWCQLFSEPGAGSDLASLSTRAEQDGDTWVVTGQKVWCSNGRVADKGI
                     LLARTDPDAPKHKGISFFLVDMRAPGVETRPLRQLTGDSEFDEVFLDEVRIPDNDLVG
                     PLHGGWGVAMSTLTNERGHIGA"
BASE COUNT          348 a          930 c          895 g          340 t
ORIGIN      
        1 gggttcttgt cgccgctcag ccggtacagc agcgcctgct gcggcagcgt cggcgacacc
       61 acggtgaagt ccgcgggccg ctcgggggcg tcgttgccgg gctgcgggcc gggctcgccg
      121 ccgaagccgc cctcgccgcg caggaacagc gagtagcggt tggtgaacag cgcctcgccg
      181 ccctcctcgc gcgtgtcgac gtccaggacc agcagcgcgg ccttgccctt gtcgtacagg
      241 cccgcgaccc gcccggaagt gaccacgtcc gccgacgtgg gcagcggccg gtggatggtg
      301 aggtcctgct cgccgtgcag cagcgtcgcc cagttgatct ccaggcccgg gatctccagc
      361 atgccggcca gcgccggggc cgcggcgatc acgccgaagg tcggcaggac cttcaggccg
      421 ccctcgtagg tgtaggcgag ctcgcgcggg tccgtcggcg gatcgcccgc ccccacgccc
      481 aggtggtaca ggatcacgtc gtcgggcgcc cacgagcccg tcacgggggg cagttcggcg
      541 ccgagggcct tctccgggtc gaccggcatc gcgcgggtcc tttctcagat gccgagacgc
      601 tgcgccagca gctcccggtg gtgggcgggg tcaccgaaca gcaactccga gctcttcgcg
      661 cgcttgaagt acaagtgcgc ggggtgctcc caggtgaagc cgatgccgcc gtggatctgg
      721 atggtgtcgg cggcggcctt gaagtaggcc tccgagcagt acgccttggc catggcggcg
      781 gccacgggca ggtccgcgtc cagctcggcc gcgcaccagc cggcgtagta ggccgcggac
      841 ttggcctgct ccaccgcgac cagcatgtcg gcgcaacggt gcttgacggc ctggaacgag
      901 ccgatcggtc ggccgaactg gatgcgctcc ttggcgtagg ccaccgacag gtccaggcag
      961 cgttgcgcgc cgccgacctg ctcggcggcc agcgccacgg ccgccaggtc cagcacgcgc
     1021 tcgaccacct gccagccgcc gccctcctgg ccgaccagcc gggcgggcac cgcgtcgaac
     1081 tccaggcggg cgagcttgcg cgtctggtcc atggtcgaaa ggaccgtgcg cgccaggccc
     1141 ggcgcgtcgc ccggcaccgc gaacagcgtg acgcctccct ccgtgcgcgc ggcgaccagc
     1201 accaggtcgg cgacgtggcc gtccagcacg tagctcttgt ggccgtgcag cgtccagcgc
     1261 tcgccgtcgc ggcggacctc gagctgcacg ccctcggcgt cccagcggcc gttgtcctcc
     1321 gtgagcgcga gcgtcgcgac ggtgtcgccg gcggcgatgc ccggcagcca ttccttgcgc
     1381 gccgcgtcgt cgcccgcgtg cagcagcgtc gagacgccga gcacgacggt ggagaagtac
     1441 ggcgcgcaca gcagcgccgc gcccatctgc tccagcacca cgaccagctc caggaacgag
     1501 tagccggcgc cgccgtgctc ctcgggcacg atcaggccct gcaggcccat ctgctcggcc
     1561 atctggcgcc acaccgccgg gtcgtagccc tgctcggtgt ccatgagccg gcgcacctcc
     1621 ggctcgccgg acttgtcctc caggaactgg cggacgatcc ggccgagctc gagctgctcg
     1681 tcactgaagg cgaagttcat cgatccctcc cgggcgccgc gggcttccgc cacgcgtggt
     1741 gtcggtgtat gttatcccgt attcacgtaa ccccgcaaga gcgaggagac cgaagtggcc
     1801 gacgcgaccg agcagccccg cgagcgggtg cgcgacctca cccggcgcct ggtggcggca
     1861 cacgacccgg cgacgacgcc cgccgaggag ctctggggcg cgcagttcga cgcgggcctg
     1921 gcctggatcc acttccccga gggccgcgga gggctgggac tgccgcccga cctccagatg
     1981 gtggtggacg aggagctgcg ggcggccggc gcgccgagca acgtgctgcg caacttcatg
     2041 ggcgtcggca tggccgggcc cacgctggtg gcgcacggca cgccggagca gcaggaacgc
     2101 ctcctgcggc ccctgttcac ctgcgaggag atctggtgcc agctgttcag cgagcccggc
     2161 gccggctccg acctggcgag cctctccacc cgggcggagc aggacggcga cacgtgggtg
     2221 gtcaccggcc agaaggtgtg gtgctcgaac ggcagggtcg ccgacaaggg gatcctcctc
     2281 gcccgcaccg accccgacgc tccgaagcac aagggcatca gcttcttcct cgtcgacatg
     2341 cgcgcgcccg gcgtcgagac gcggccgctc cgccagctca ccggcgacag cgagttcgac
     2401 gaggtcttcc tcgacgaggt ccgcatcccc gacaacgacc tcgtcggccc gctccacggc
     2461 ggctgggggg tggccatgtc gacgctgacg aacgagcgcg gccacatcgg tgc
//
LOCUS       DATMKC010000130         4080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_184971, whole genome shotgun sequence.
ACCESSION   DATMKC010000130 DATMKC010000000
VERSION     DATMKC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184971"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(395..1813)
                     /gene="glnA"
                     /locus_tag="VNU01_03910"
     CDS             complement(395..1813)
                     /gene="glnA"
                     /locus_tag="VNU01_03910"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWH31796.1"
                     /translation="MGTPKDVIKQIADEGIEFVDFRFCDLPGLMQHYTIPARVLDESV
                     FEEGLGFDGSSIRGFQEIQESDMLLVPDATTTYVDEFRQHKTLNIHCFVRDPLTGESY
                     SRDPRYVATKAEAYLKGTGIADTAYFGPEAEFFIFDEVRFDTNAHESYYHVDSIEGAW
                     NSGRAEGPNLGYKPRHKEGYFPVPPMDHFQDLRSQMVLHLERAGIEVELQHHEVGTAG
                     QAEIDIKFDTLLRTADKMMTFKYIIKSTAAAAGRTVTFMPKPIFGDNGSGMHTHQSLW
                     NGGDPLFFDENGYAQLSDTARWYIGGLLAHAPALTAFTNPTTNSYRRLVPGFEAPVNL
                     VYSQRNRSAACRIPLVSNSPKAKRVEYRVPDPSCNPYLAFSALLMAGLDGVRNKIEPP
                     DPVDKDIYELPREELESLPGVPASLEEALEALRGDHEFLLEGGVFTQDLIDTWIDYKM
                     NREVSQIRLRPHPYEFHLFYDI"
     gene            complement(2034..3089)
                     /locus_tag="VNU01_03915"
     CDS             complement(2034..3089)
                     /locus_tag="VNU01_03915"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWH31797.1"
                     /translation="MRLLLTQSLTTFTLAQASAFSPEAQAACTPANPPDGFEPNFVCQ
                     QVFEWTQLRWLALASGTIFKVVFVLVLAWFATRLVRRALKRFAERVTREGMDSGRLVK
                     VMSPLADTAPINLQRAQMRARTITGVIRSFAVVAIWITAGITVLGAFNINLGPLIAGA
                     GIAGVALGFGAQNLVRDFLSGIFMLLEDQYGVGDVINAGEATGIVESVSLRTTRLRSV
                     DGTVWYVPNGEIKRVGNMSHQWSRALLDIGVAYDTDLDLATRVIQATANAMAEDPEWA
                     PLILSEPEVWGLEQWGESQLTIRLVVQTVPLEQWNVSRELRRRLKEAFDEAGIEIPFP
                     QRTVWIRNPGEEAGTGI"
     gene            complement(3093..4043)
                     /gene="lipA"
                     /locus_tag="VNU01_03920"
     CDS             complement(3093..4043)
                     /gene="lipA"
                     /locus_tag="VNU01_03920"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWH31798.1"
                     /translation="MTTLIPEGATRLTLVGVPQVPQRKPEWLRQHVPLAGPGYRDIKE
                     TMRGLTLHTVCEEAACPNIHECWEAREATFLIGGDQCTRACGFCAIATGKPGMLDRHE
                     PLRVAEAVRAMGLRFAVVTGVARDDLSDGGAWLYAETLRQIRALVPGCGVELLVPDFG
                     GVQEHIRKITDEAPEVLAHNLETVRSRFKHIRRGFRYDRTLEVLALMRRLLPPGSAVK
                     SNLMLGLGETEQEVLASMRDLRAAGVEILTIGQYLPPTHTHAALQRYAPPEEFDRYRQ
                     AGAEMGFAHVESGPLVRSSYRAGRQAEAAGVWGAVASGMT"
BASE COUNT          577 a         1400 c         1446 g          657 t
ORIGIN      
        1 cgtgcgggcc gagcagcggc gcctgtcgcg ccacaaggtc gagcacgtgg ccgacgagcc
       61 ggtggacgcg cccggcgggg tctggctggg cttcaccgac ccgtggggca accgcctggg
      121 ctactggcag gaacgcgacg aggactaggc gtatcggccg aggttgacgg ctccggggct
      181 cgggtaggcc tctttacgcg acctgccccc gatccacccg aggaggagcc gtgtacatcg
      241 gtctcggcac cctggccgtc atcatcatcc tgatcctgct gctgacctag cgcaggatgc
      301 gatgaagggg cccgccggtt ctccggcggg ccccttcatg tgcgctgtgg gacgacttgt
      361 gtccggctat ggacgcaagt cgtcccacaa gggtctagat gtcgtagaag aggtggaact
      421 cgtaggggtg cgggcgcagg cggatctggg acacctcgcg gttcatcttg tagtcgatcc
      481 aggtgtcgat caggtcctgc gtgaacacgc cgccctcgag caggaactcg tggtcgccgc
      541 gcagcgcctc cagggcctcc tccaggctcg ccggcacgcc gggcagggac tccagctcct
      601 cgcgcggcag ctcgtagatg tccttgtcca ccgggtccgg cggctcgatc ttgttgcgca
      661 cgccgtccag gcccgccatg agcagcgccg agaacgcgag gtaggggttg caggacgggt
      721 ccggcacccg gtactccacg cgcttggcct tcggcgagtt gctgaccagc gggatgcggc
      781 acgcggccga gcggttgcgc tggctgtaga ccaggttcac cggcgcctcg aagcccggca
      841 ccagacggcg gtagctgttg gtggtcgggt tggtgaacgc ggtcagcgcc ggcgcgtggg
      901 ccagcaggcc cccgatgtac cagcgcgcgg tgtcggagag ctgcgcgtag ccgttctcgt
      961 cgaagaacag cgggtcgccg ccgttccaca gcgactggtg ggtgtgcatg ccggagccgt
     1021 tgtcaccgaa gatcggcttg ggcatgaacg tcaccgtgcg gcccgccgcg gcggccgtgg
     1081 acttgatgat gtacttgaac gtcatcatct tgtccgcggt gcgcagcagc gtgtcgaact
     1141 tgatgtcgat ctcggcctgg ccggcggtgc cgacctcgtg gtgctgcagc tccacctcga
     1201 tgccggcccg ctccaggtgc aggaccatct gggaccgcag gtcctggaag tggtccatcg
     1261 gcggcaccgg gaagtagccc tccttgtggc gcggcttgta gcccaggttg gggccctcgg
     1321 cgcgaccgga gttccacgcg ccctcgatcg agtcgacgtg gtagtaggac tcgtgcgcgt
     1381 tggtgtcgaa gcgcacctcg tcgaagatga agaactccgc ctcggggccg aagtaggccg
     1441 tgtcggcgat gccggtgccc ttgaggtacg cctccgcctt ggtggcgacg tagcgcgggt
     1501 cgcggctgta gctctcgccc gtgagcgggt cgcgcacgaa gcagtggatg ttcagcgtct
     1561 tgtgctggcg gaactcgtcc acgtacgtcg tggtcgcgtc cggcaccagg agcatgtcgg
     1621 actcctggat ctcctggaag ccgcggatcg acgagccgtc gaagccgagg ccctcctcga
     1681 acacgctctc gtccagcacg cgtgccggga tggtgtagtg ctgcatcagg cccggcaggt
     1741 cgcagaaccg gaagtcgacg aactcgatgc cctcgtccgc gatctgcttg atgacgtcct
     1801 ttggagtccc caacgttgct cctttcgtga ttcacgctgc gtgccgtacc cggttggcag
     1861 gtccggcgct cgggtggttc gccccccgag tggcgtccgg ggccgacagg cggccccagt
     1921 cgaccactgt tggggccagg gggcgaaccg ccccagcgcc gcgtctgccg tttcggtggc
     1981 agcaccgtag gcaggggggg tgtcgccgcc gttgctcgaa tgtttccagg ctgttaaatg
     2041 cccgttcccg cctcctcgcc cgggttgcgg atccacacgg tgcgctgggg gaaggggatc
     2101 tcgatgccgg cctcgtcgaa ggcctccttg agccgccggc gcagctcgcg gctgacgttc
     2161 cactgctcca gcggcacggt ctgcaccacc aggcggatgg tcagctgcga ctcgccccac
     2221 tgctccaggc cccacacctc cggctcgctg aggatcagcg gcgcccactc cgggtcctcg
     2281 gccatggcgt tcgcggtggc ttggatgacg cgcgtcgcga ggtcgaggtc ggtgtcgtag
     2341 gccacgccga tgtccagcag cgcccgcgac cactggtggc tcatgttgcc gacgcgcttg
     2401 atctcgccgt tgggcacgta ccagaccgtg ccgtccacgg agcgcaggcg cgtggtgcgc
     2461 aggctcaccg actccacgat cccggtggcc tcgcccgcgt tgatcacgtc gccgaccccg
     2521 tactggtcct ccagcagcat gaagatgccc gacaggaagt cgcgcacgag gttctgcgcg
     2581 ccgaagccca gcgccacgcc ggcgatgccg gcgccggcga tgagcgggcc caggttgatg
     2641 ttgaacgcgc ccagcacggt gatccccgcg gtgatccaga tggccaccac cgcgaagctg
     2701 cggatcacgc cggtgatcgt gcgcgcgcgc atctgcgcgc gctggaggtt gatcggcgcc
     2761 gtgtccgcca gcggcgacat caccttcacc aggcggccgg agtccatgcc ctcccgggtc
     2821 acccgctccg cgaagcgctt cagcgcccgg cgcaccagcc gcgtggcgaa ccacgccagc
     2881 accaggacga agaccacctt gaagatcgtc ccagaggcca gtgccagcca gcgcagctgc
     2941 gtccactcga agacctgctg gcacacgaag ttcggctcga agccgtccgg cgggttggcc
     3001 ggggtgcagg cggcctgtgc ctccggcgag aaggcggagg cctgagcgag cgtgaacgtg
     3061 gtgagtgact gcgtcagcag aaggcgcatg ggtcaggtca taccagacgc caccgcgccc
     3121 cacacgcccg cggcctcggc ctggcgaccg gcacggtagg aggagcgcac cagcggacct
     3181 gactccacgt gggcgaagcc catctcggcg cccgcctgcc ggtagcggtc gaactcctcc
     3241 ggcggcgcgt agcgctgcag cgcggcgtgc gtgtgcgtgg gcggcaggta ttggccgatc
     3301 gtgaggatct ccacgcccgc cgcgcgcagg tcgcgcatcg acgccagcac ctcctgctcc
     3361 gtctcgccca ggcccagcat caggttggac ttcacggcgc tgcccggcgg cagcagccgg
     3421 cgcatcagcg ccaggacctc cagcgtgcgg tcgtagcgga agccgcggcg gatgtgcttg
     3481 aagcggctgc gcacggtctc gaggttgtgc gccagcacct ccggcgcctc gtcggtgatc
     3541 ttgcggatgt gctcctggac gccgccgaag tcggggacca gcagttccac gccgcagccg
     3601 ggcaccagcg cgcggatctg gcgcagcgtc tcggcgtaca gccaggcgcc gccgtcggac
     3661 aggtcgtcgc gcgcgacgcc ggtcaccacg gcgaagcgca gccccatggc gcgcaccgcc
     3721 tcggcgacgc gcagcggctc gtggcggtcg agcatccccg gcttgcccgt ggcgatggcg
     3781 cagaagccgc acgcgcgcgt gcactggtcg ccgccgatga ggaacgtggc ctcgcgcgcc
     3841 tcccagcact cgtggatgtt ggggcaggcc gcctcctcgc acacggtgtg cagggtcagc
     3901 ccgcgcatcg tctccttgat gtcgcggtag ccggggcccg ccagcgggac gtgctggcgc
     3961 agccactcgg gcttgcgctg gggcacctgc ggcacgccca cgagcgtgag ccgtgtggcg
     4021 ccttcgggga tgagcgtggt cactggcctc gcctcgtcag atcctcaccg ggtccatgcc
//
LOCUS       DATMKC010000131         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_339856, whole genome shotgun sequence.
ACCESSION   DATMKC010000131 DATMKC010000000
VERSION     DATMKC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339856"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..803
                     /locus_tag="VNU01_03925"
     CDS             204..803
                     /locus_tag="VNU01_03925"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWH31799.1"
                     /translation="MAINAYARSAADAVLRPVVAGLVRVGATADAMTVLGLLTVLIGA
                     GLVAAGQRQAGALVVLAGALLDALDGGVARARGTQRPFGAFLDSVTDRVADVAVFGAV
                     AWLVRDDAVLFTVTLVALGAAQVTSYMRAKAESLGWHATVGVVERAERLGILLLGIFF
                     APLLVPAVWLLAVGGVVTIAQRFATVARQAREPGPAGGA"
     gene            800..1819
                     /locus_tag="VNU01_03930"
     CDS             800..1819
                     /locus_tag="VNU01_03930"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HWH31800.1"
                     /translation="MSYRGTGVPGVPVDRNPGAADRIPPDPRPEPLRLRLTGWLYVAV
                     WEAARRLPESWAHALAQAAARWMLRRRGPAQERLRRNLARVVPADELDDTVAEAFRSY
                     ARYWVEAFRAADLSAHDVDRRTTSTGFDKLDAVLEQGRGAIVLLAHHGSWDVAAQWGE
                     THGYHLAVVAELLRPRAVFRRFVRLREAIGLEVVPLRRGDDMVGRLTEVLAANHMVGL
                     LSERDLTGKGPVVDFFGEPARVPPGPVVLSQRTGVPIVPAVLLQRPGLRWHFEILDPF
                     DVEGMTVAEGCQRLAHALEDIIRTDPAQWHAFQPVWLADLPPRHGPDKRGDWRPPDGA
                     SGAAS"
     gene            1816..2928
                     /locus_tag="VNU01_03935"
     CDS             1816..2928
                     /locus_tag="VNU01_03935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWH31801.1"
                     /translation="MKIALVCPYDWSRHGGVQAHIAALRAHLGADHEVRVFAPASQPV
                     RDRDVVVVGRPLPIRYNRSVAPVALSPLAARRVVRALREFSPHVTHVHEPLSPVVSAA
                     AAAFGPRPIVGTFHAWSSSDRLYRAAAPAVRRVAERLSVRIAVSPAAQSYAGQALGLP
                     LGAFRLVPNGVATANYRDATPIPELVDPERPLLLFVGRLEPRKGLDVLVRAYLRLRAS
                     RPRLRLCVVGEGPDRQRCQEMIPDSVRPDVLFVGAVSEEEKPRYHASADLYVAPNTGG
                     ESFGIVLLEGMAAGLPVVASDIPGFRTVLKDGRQGRLVGPSDAFALAEAIDTLLSNDK
                     LRLAMAAEGRRTAAEYDWGVVSARILELYREAMTGA"
     gene            3072..>3576
                     /locus_tag="VNU01_03940"
     CDS             3072..>3576
                     /locus_tag="VNU01_03940"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HWH31802.1"
                     /translation="MLVALFAVALTLTACGGGEPEPEPTPSETEPTPSPTPEPELAPL
                     TGEEVESTGILDRPVVAVKIDNASPARPQAGLGAADVVFEELVEGGYTRFLALYQSQA
                     PETAGPVRSGRDVEADLLPPYQPLFVISGAAEPTYAVLRGAGLKIREEGQPEDAFQRA
                     SDRKRPYN"
BASE COUNT          372 a         1355 c         1383 g          466 t
ORIGIN      
        1 cggcggcggc ggggcgggcc tcggcgacgt gctgggcgag gtggccgcgc tggggatgtt
       61 cgcggcggtg ctgctgccgc tggcggtgtg gcgcctcgcg cgcaccggct gaagtgcccc
      121 gctccgggac gacttgtgtc ccatagcggc cacaagtagt cccgcagcga agagggcggc
      181 ggcggtggcg gtacgctgcg ggcatggcca tcaacgccta tgcccgctcg gccgcggacg
      241 cggtcctgcg ccccgtcgtg gcgggcctgg tccgcgtcgg cgccaccgcc gacgccatga
      301 ccgtgctggg actgctcacc gtgctcatcg gcgccggcct cgtcgcggcg gggcagcgcc
      361 aggccggtgc gctcgtcgtg ctcgccggcg cgctgctgga cgccctggac ggcggcgtcg
      421 cgcgcgcccg cggcacgcag cgcccgttcg gcgcgttcct ggacagcgtc accgaccgcg
      481 tcgccgacgt cgccgtgttc ggcgccgtcg cgtggctggt ccgcgacgac gccgtgctgt
      541 tcaccgtgac gctggtcgcc ctgggcgccg cccaggtcac cagctacatg cgcgcgaagg
      601 ccgagtcgct cgggtggcac gccaccgtcg gggtggtcga gcgcgccgag cgcctgggca
      661 tcctgctgct cggcatcttc ttcgcgccgc tgctcgtgcc ggccgtgtgg ctgctcgcgg
      721 tcggcggcgt cgtcaccatc gcgcagcgct tcgccacggt ggcccgccag gcgcgcgagc
      781 cgggaccggc ggggggcgcg tgagctaccg aggcaccggg gtccccggcg tgccggtgga
      841 ccgcaatccc ggcgcggcgg accgcatccc gcccgacccc cgtccggagc cgctgaggct
      901 gcggctgacc ggctggctgt acgtggccgt gtgggaggcc gcgcgccggc tgccggagtc
      961 gtgggcgcac gcgctggcgc aggccgccgc gcgctggatg ctgcggcgcc ggggcccggc
     1021 ccaggagcgg ctgcgccgca acctcgcgcg cgtggtcccc gccgacgagc tcgacgacac
     1081 ggtggccgag gcgttccgtt cctacgcccg ctactgggtg gaggcgttcc gcgccgctga
     1141 cctgtccgct cacgacgtcg accggcgcac caccagcacc ggcttcgaca agctcgacgc
     1201 ggtgctggag cagggccgcg gcgccatcgt gctgctggcg caccacggct cctgggacgt
     1261 ggcggcgcag tggggcgaga cgcacggcta ccacctcgcc gtggtcgcgg agctgctgcg
     1321 cccccgcgcg gtgttccgcc gcttcgtgcg gctgcgcgag gcgatcggcc tggaggtggt
     1381 gccgctgcgg cgcggcgacg acatggtcgg ccgcctcacg gaggtgctgg ccgccaacca
     1441 catggtgggc ctgctgtcgg agcgcgacct caccggcaag gggccggtcg tcgacttctt
     1501 cggcgagccg gcgcgcgtgc cgccggggcc cgtcgtgctc agccagcgca ccggcgtgcc
     1561 gatcgtgccg gccgtgctgc tgcagaggcc gggcctgcgc tggcacttcg agatcctgga
     1621 cccgttcgac gtggagggca tgacggtggc ggagggctgc cagcgcctgg cgcacgccct
     1681 ggaggacatc atccgcaccg accccgccca gtggcacgcg ttccagccgg tgtggctggc
     1741 ggacctgccc cctcggcatg ggccggacaa gcgcggcgac tggcggccgc ccgacggcgc
     1801 gagcggggcg gcgtcgtgaa gatcgccctg gtctgcccgt acgactggtc gcgccacggc
     1861 ggcgtgcagg cgcacatcgc cgcgctgcgc gcgcacctgg gcgccgacca cgaggtgcgc
     1921 gtgttcgccc ccgcgtcgca gccggtgcgc gaccgcgacg tcgtggtggt cgggcggccg
     1981 ctgccgatcc gctacaaccg ctcggtggcg cccgtggcgc tgtcgccgct ggccgcccgc
     2041 cgcgtcgtgc gcgcgctgcg ggagttctcg ccgcacgtca cccacgtcca cgagccgctg
     2101 tcgccggtgg tctcggcggc cgccgcggcg ttcggcccgc ggccgatcgt cggcacgttc
     2161 cacgcgtgga gctcctcgga ccggctgtac cgcgcggcgg ccccggcggt gcgccgcgtc
     2221 gccgagcggc tgtcggtgcg catcgcggtg tccccggcgg cgcagtccta cgccggccag
     2281 gcgctgggcc tgccgctggg cgcgttccgg ctggtgccca acggcgtggc cacggccaac
     2341 taccgcgacg cgacgcccat cccggagctg gtcgacccgg agcggccgct gctgctgttc
     2401 gtcggccgcc tggagccgcg caagggcctg gacgtgctgg tgcgcgcgta cctgcgcctg
     2461 cgcgcgtcgc gcccgcggct gcgcctgtgc gtggtggggg agggcccgga ccgccagcgc
     2521 tgccaggaga tgatccccga ctcggtgcgc cctgacgtgc tgttcgtcgg cgcggtgagc
     2581 gaggaggaga agccgcgcta ccacgccagc gcggacctct acgtcgcccc gaacaccggc
     2641 ggggagagct tcggcatcgt cctgctggag ggcatggcgg cgggcctgcc ggtggtggcc
     2701 tccgacatcc ccggcttccg cacggtgctc aaggacggcc ggcaggggcg cctggtgggc
     2761 cccagcgacg cgttcgcgct ggcggaggcg atcgacacgc tgctgtccaa cgacaagctg
     2821 cgcctggcga tggccgccga gggccgccgc accgccgccg agtacgactg gggcgtggtc
     2881 agcgcccgca tcctggagct gtaccgcgag gcgatgaccg gcgcgtaggg cgcgccgggc
     2941 gccccgccgg gccgccggca cggcccccct gaccggacgg ccgggcctgc cccccggtgc
     3001 cccggccgta cactcgctcg gcaagccggt cggaccccaa cccccgcaag gagtgcttca
     3061 ccgtgcgccg aatgctggtc gcgctgttcg ccgtcgccct cacgctgacc gcctgtggcg
     3121 gcggcgagcc ggagccggag cccacgccgt cggagaccga gccgacgccc tcgccgacac
     3181 ccgagccgga gctcgcgccc ctgaccggcg aggaggtcga gtccacgggc atcctggacc
     3241 gccccgtggt ggccgtgaag atcgacaacg cctcgcccgc gcggccgcag gccggcctgg
     3301 gcgccgccga cgtggtgttc gaggagctcg tggagggcgg ctacacccgc ttcctggcgc
     3361 tgtaccagtc ccaggcgccg gagacggccg ggccggtgcg ctccggccgc gacgtcgagg
     3421 cggacctgct gccgccgtac cagccgctgt tcgtgatctc cggagccgcc gagccgacgt
     3481 acgcggtgct gcgcggcgcg ggcctgaaga tccgcgagga gggccagccg gaggacgcct
     3541 tccagcgcgc gtcggaccgc aagcgcccct acaacc
//
LOCUS       DATMKC010000132         3026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_246662, whole genome shotgun sequence.
ACCESSION   DATMKC010000132 DATMKC010000000
VERSION     DATMKC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246662"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VNU01_03945"
     CDS             complement(<1..1408)
                     /locus_tag="VNU01_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HWH31803.1"
                     /translation="MLFRPDRTDFRLIGFYTGRVVGGVGLAMLVPTVLAFALGEHSDG
                     FGFLIGASVALIAGSLGTLLLRTRAPLRTTHGLATVALAWLVAPLPAAIPFILSGHYA
                     SFLDAYFEAMSGFATIGLTLANDLDHMSVSVNLWRHLTHFLGGQGIVIVVLTIFAAGG
                     GHVGSLYTGEGREEKIVPNVISTARFIWKVALVYGLLGTTLLWVAMLFAGLQPLSALY
                     HAASAFMASFDTGGFAVMSSSAAFYRSPAVELVLIVLMLAGAFSFALHYQLWRGRQSE
                     LYRNIETRTMALSILMLFTFALIGLARTGTFEHWSELFRHGFFQIVSGHTSTGLGTVP
                     GRLFLTDWGMLAPAMLVTAMAIGGMAGSTAAGIKAIRVGVVLKGLRADIRRVLLPEHA
                     AVVETFHSTTKRILRAEQVRQASMLLVLFVLMYLLGGLLGLFYGYDMRLALFESTAAG
                     SGAGFSVGVVRPALEWPIK"
     gene            complement(1413..1592)
                     /locus_tag="VNU01_03950"
     CDS             complement(1413..1592)
                     /locus_tag="VNU01_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31804.1"
                     /translation="MTANPPHPPGPGEPARGGRAEFEGNPLSGGTQGILAVTLATLGL
                     ALAAAAIAGVVAWLV"
     gene            complement(1589..2257)
                     /locus_tag="VNU01_03955"
     CDS             complement(1589..2257)
                     /locus_tag="VNU01_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWH31805.1"
                     /translation="MYVVIAGGGKVGRAIAHDLIEEGHDVAIIEKVEHRCEQLLRDHD
                     LLVIHGDACDVRYLEQARPERADVFAATTGDDDDNFVSCQLALTTFGVPRAIARVNTP
                     RNEEIFERLGIEAVSTTTLISRLIREEVTVGELIHLTTLRGGKVNLVELDIPDDAPGR
                     PRLVSELHLPKQSVLVCIFRDEQTIIPRGETPLLAGDQVIALTAPELEEALRAAVLGE
                     DERR"
     gene            complement(2259..2924)
                     /locus_tag="VNU01_03960"
     CDS             complement(2259..2924)
                     /locus_tag="VNU01_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWH31806.1"
                     /translation="MHIIIGGCGRVGAELAERLSADGHDVVIVDARDSSFDRLGGGFN
                     GETLVGDITDKDVLVRAGIQGSDALIAVTDLDNANLMCVQIATELFGLRNTVARLFNP
                     QREASYKKMGVHYVSGTRLVAKAILNEIRAGTFPHHIAFEDSDIQVVEMRVHRDGHGA
                     TIAELEAGGGVRVAAFRSGVRVRIPRQDDRVREDDIVVAAVSQGAYRHLGKFAEHPLA
                     GGA"
BASE COUNT          470 a         1071 c         1081 g          404 t
ORIGIN      
        1 ccttgatcgg ccattccagg gcgggccgca cgacccccac ggagaacccg gcgccgctgc
       61 cggccgcggt gctctcgaac agcgccaggc gcatgtcata gccgtagaac agcccgagca
      121 ggccgccgag caggtacatc agcacgaaca gcaccagcag catcgacgcc tgccgcacct
      181 gctcggcgcg gagaatgcgc ttggtggtgg agtggaaggt ctccaccacg gcggcgtgct
      241 cgggcagcag gacccggcgg atgtccgcgc gcaggccttt gagcaccacg ccgacgcgga
      301 tcgccttgat gcccgcggcc gtggacccag ccatgccgcc gatcgccatc gcggtcacca
      361 gcatcgccgg cgcgagcatg ccccagtcgg tcaggaacag ccgcccgggc accgtcccca
      421 ggccggtcga ggtgtggccg ctgacgatct ggaagaagcc gtggcggaac agctcgctcc
      481 agtgctcgaa cgtgcccgtg cgcgcaaggc cgatgagcgc gaacgtgaac agcatgagga
      541 tggacagcgc catcgtccgc gtctcgatgt tgcgatagag ctcgctctgg cggccgcgcc
      601 acagctgata gtgaagggcg aagctgaacg cgccggcgag catgagcacg atcagcacga
      661 gctccaccgc gggcgagcgg tagaaggcgg cgctcgacga catcaccgcg aagccgccgg
      721 tgtcgaacga agccatgaac gccgacgcgg cgtgatacag cgcggacagc ggctggaggc
      781 cggcgaacag catcgccacc cacagcagcg tcgtccccag caagccgtag accagcgcca
      841 ccttccagat gaagcgggca gtggagatga cgttcggcac gatcttctcc tcgcgcccct
      901 cgccggtgta cagggaaccg acgtgccccc cgccggccgc gaagatcgtc agcaccacga
      961 tcacgatgcc ctgcccgccc aggaagtgcg tgagatgccg ccagaggttc acgctgacgc
     1021 tcatgtggtc caggtcgttg gccagcgtga gcccgatcgt ggcgaacccg ctcatggcct
     1081 cgaagtaggc gtcgaggaag gacgcgtagt ggccggagag gatgaacggg atggcggccg
     1141 gcagcggggc gacgagccat gccagcgcca cggtggccag gccgtgcgtg gtgcgcaggg
     1201 gcgcgcgcgt gcgcaggagc aacgtgccca gcgagccggc gatcagagcc accgaggcgc
     1261 cgatgaggaa gccgaagccg tcgctgtgct cgcccagggc gaaggccagc accgtgggga
     1321 cgagcatggc caggcccacg ccgcccacca cccggccggt gtagaagccg atcaggcgga
     1381 agtccgtgcg gtccggacgg aacagcacgc cgctacacca gccaggcgac gacgccggcg
     1441 atggccgcgg ccgccagcgc cagccccagc gtggcgagcg tgaccgcgag gatgccctgg
     1501 gtgccgccgg acagcgggtt gccttcgaac tcggcgcggc cgccgcgcgc cggctcgccc
     1561 ggcccggggg ggtgcggggg gttggccgtc atcggcgctc gtcctcgccc agcacggcgg
     1621 cgcgcagcgc ctcctccagc tccggggcgg tcagggcgat gacctggtcg ccggccagca
     1681 gcggggtctc gccgcgcggg atgatggtct gctcgtcgcg gaagatgcac acgaggacgg
     1741 actgcttcgg cagatgcagc tcgctcacca gccgcggcct gccgggcgcg tcgtcgggga
     1801 tgtccagctc caccaggttg accttgccgc cgcgcagcgt ggtcaggtgg atgagctcgc
     1861 cgacggtgac ctcctcgcgg atcagccggg agatcagcgt ggtggtggag acggcctcga
     1921 tgcccagccg ctcgaagatc tcctcgttgc gcggggtgtt gacccgggcg atcgcgcgcg
     1981 gcaccccgaa cgtggtgagc gcgagctggc aggacacgaa gttgtcgtcg tcgtcgccgg
     2041 tcgtggcggc gaacacgtcg gcgcgctcgg gccgcgcctg ctccaggtag cgcacgtcgc
     2101 aggcgtcgcc atggatgacc agcaggtcgt ggtcgcgcag cagctgctcg cagcggtgct
     2161 ccaccttctc gatgatggcc acgtcgtggc cctcctcgat gaggtcgtgc gcgatggcgc
     2221 gccccacctt gccgcctccg gcgatcacca cgtacatgtc aggcgcctcc ggccagcggg
     2281 tgctcggcga acttgccgag gtgccggtag gcgccctggc tgaccgcggc caccacgatg
     2341 tcgtcctcgc gcacgcggtc gtcctggcgc gggatgcgca cgcgcacccc gctgcggaac
     2401 gccgccacgc gcacgccccc gccggcctcc agctcggcga tcgtcgcgcc gtggccatcg
     2461 cggtgcacgc gcatctcgac cacctggatg tcgctgtcct cgaaggcgat gtggtgcggg
     2521 aacgtgcccg cgcggatctc gttgaggatc gccttggcca ccagccgcgt gcccgacacg
     2581 tagtgcacgc ccatcttctt gtaggacgcc tcgcgctgcg ggttgaacag ccgcgcgacc
     2641 gtgttgcgca ggccgaacag ctccgtggcg atctgcacgc acatcaggtt cgcgttgtcc
     2701 aggtccgtca cggcgatcag cgcgtccgag ccctggatgc cggcgcgcac caggacgtcc
     2761 ttgtcggtga tgtcgccgac cagggtctcg ccgttgaacc cgccgcccag gcggtcgaac
     2821 gaggagtccc gggcgtccac gatcacgacg tcgtggccgt cggcggacag gcgctcggcc
     2881 agctcggcgc cgacgcgccc gcagccgccg atgatgatgt gcacgccttg ccctcctgtc
     2941 gcggcgaccg ggtcggtcga ccggccagta tgccccggag ccgttgccag cgcggccgga
     3001 tgccgcgtcc cggggcgtcc ctatac
//
LOCUS       DATMKC010000133         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_100420, whole genome shotgun sequence.
ACCESSION   DATMKC010000133 DATMKC010000000
VERSION     DATMKC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100420"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VNU01_03965"
     CDS             complement(<1..565)
                     /locus_tag="VNU01_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31807.1"
                     /translation="MTSLTALLSAAAALGGVLAVAGSWSRGIRPGAIPHLSVGAVAGV
                     GSGVALQFAQAPALLTWMAAAAAGALCGAALHQAGHAPPRPDREVPVGGDLVTLAGLL
                     GVAALLAPPGPGAGTRTLLGGQWPAVDGSSLLASLAVLALGAGLAAALALAPAAVLPL
                     RARSVLAGAGTAALAAAAAQGAAAAHAY"
     gene            703..>2790
                     /locus_tag="VNU01_03970"
     CDS             703..>2790
                     /locus_tag="VNU01_03970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWH31808.1"
                     /translation="MWRRLALLATTLLAVTLALALGAATVVPALARSVAAMSGLKVTM
                     PTAADLSLAALEERSVVYAADGSVLAVLHDEVDRRIVPLEEIPEHVRDAVITAEDQKF
                     WEHGGYDVEGIARAAIANAKARDVLQGGSTITQQLAKSLVGNQRTLERKLSELTYAVA
                     LESRYTKEELLERYLNQVYLGAGAYGVAAAAEEYFHKQPSELTPAEGAMLAAVIRSPS
                     RLDPRRNGARVKVRRDAVLRAMAGNGLLHGAEAQAALSSPLGVLPHRPSEVREPYFVE
                     AMKREFFANPAFGATREERINLLFSGGLQIQTTLRPELQAAAEEVIGQFFPEPGGPTA
                     AIATVDPRDGRVLALASGVKFTEEQFDLAAQGRRQPGSSFKPFVLAAALEYGFPLDMR
                     LRGDGPISIDMPGAQEDWEVDNYAGSSYGDLDLRSALTRSVNTAFAQLVMLVGPAAAA
                     DVAERLGINVTAALGGLPPYPAIALGGLQVGVTPLEMASAYGTLAVAGQRVAPRVIER
                     VTNRAGDVLWQARPRPETVIEPAVAGTMIDVMQDVVRRGTGTAARIRGWEPAGKTGTT
                     QNNADAWFIGTVPVMSTAVWVGHPDAQIPMRGVTGGSLTARIWQRFMAQALEGVEPVP
                     FPETPPLSSVLGKGNTQIPDVVGMEATEALRTLARAGLVGTVRSVDTTRTPGSVLWQS
                     PRDGRTARRGSTVT"
BASE COUNT          366 a         1010 c         1094 g          320 t
ORIGIN      
        1 cgtaggcgtg cgcggccgcg gcgccctggg cggcggccgc ggcgagcgcg gccgttccgg
       61 cgccggccag cacggacctg gcgcgcagcg gcaggacggc cgcgggcgcc aatgccagcg
      121 cggcggcgag cccggcgccg agggccagga ccgccaggga cgccagcagg gacgagccgt
      181 ccacggccgg ccactggccg cccaggaggg tccgcgtgcc ggcgcccggg cccggcgggg
      241 ccagcagcgc ggccacgccg agcaggcccg cgagggtgac gaggtcgccg cccacgggca
      301 cctcccggtc gggcctcggg ggcgcgtggc cggcctggtg gagggccgcg ccgcagagtg
      361 cgccggccgc ggcggccgcc atccaggtca gaagcgcggg cgcctgcgcg aactggagcg
      421 cgacgccgct gccgacgccg gcgaccgcgc ccacgctaag gtgcggtatg gcgcccgggc
      481 ggatgccccg gctccacgag ccggccactg ccaggacgcc gcccagtgcg gccgcggcgc
      541 tgagcagggc ggtgagcgac gtcatgggaa gcgcatcgta gagggcaacg gttcgacacc
      601 catggagggg ggaacgattc acccatcgtg agtactcgga cactccctgc gcttgtgcct
      661 gtccgcgacg gttccgcgac gaccgggaac cgtcgcgccc gtgtgtggcg ccgcctcgcc
      721 ctgctggcca cgaccctgct ggcggtcacg ctcgcgctcg ccctgggcgc ggcgacggtc
      781 gtgcccgccc tggcgcgcag cgtcgcggcg atgtcgggcc tgaaggtcac catgccgacc
      841 gccgcggacc tgagcctggc cgccctggag gagcgctccg tggtgtacgc cgcggacggt
      901 tcggtgctgg ccgtgctgca cgacgaggtg gaccgccgca tcgtgcccct ggaggagatc
      961 cccgagcacg tccgcgacgc ggtcatcacg gcggaggacc agaagttctg ggagcacggc
     1021 ggctatgacg tcgagggcat cgcgcgcgcc gcgatcgcca acgccaaggc gcgcgacgtg
     1081 ctgcagggcg gctccacgat cacccagcag ctcgccaaga gcctggtcgg caaccagcgc
     1141 acgctggagc gcaagctcag cgagctgacc tacgcggtcg ccctggagag ccgctacacc
     1201 aaggaggagc tgctcgagcg ctacctcaac caggtgtacc tgggcgcggg cgcgtacggc
     1261 gtggcggcgg ccgccgagga gtacttccac aagcagccgt cggagctcac gccggcggag
     1321 ggcgcgatgc tggcggccgt gatccgctcg ccgtcgcgcc tggacccgcg ccgcaacggc
     1381 gcgcgggtca aggtccgccg ggacgcggtg ctgcgcgcga tggccggcaa cggcctgctg
     1441 cacggcgcgg aggcgcaggc cgcgctgtcc tccccgctgg gcgtcctgcc gcaccgcccg
     1501 tcggaggtgc gcgagccgta cttcgtggag gcgatgaagc gcgagttctt cgccaacccg
     1561 gcgttcggcg ccacccgcga ggagcgcatc aacctgctgt tctccggcgg cttgcagatc
     1621 cagacgacgc tgcgcccgga gctgcaggcg gccgccgagg aggtcatcgg gcagttcttc
     1681 cccgagccgg gcgggcccac ggccgccatc gcgacggtgg acccgcgcga cgggcgtgtc
     1741 ctggccctgg ccagcggcgt gaagttcacc gaggagcagt tcgacctggc ggcgcagggc
     1801 aggcgccagc cgggctcgtc cttcaagccg ttcgtgctgg ccgccgcgct ggagtacggc
     1861 ttcccgctgg acatgcgcct gcgcggcgac ggccccatct cgatcgacat gcccggggcg
     1921 caggaggact gggaggtcga caactacgcg ggctccagct acggcgacct ggacctgcgc
     1981 tccgcgctca cgcgcagtgt gaacacggcg ttcgcgcagc tggtgatgct ggtgggcccg
     2041 gccgcggccg ccgacgtcgc cgagcgcctg ggcatcaacg tcaccgcggc gctgggcggg
     2101 ctgcccccct accccgccat cgcgctgggc ggtctgcagg tgggcgtgac gccgctggag
     2161 atggcctcgg cgtacgggac gctggcggtg gccgggcagc gcgtggcgcc ccgcgtgatc
     2221 gaacgggtga ccaaccgcgc cggcgacgtc ctctggcagg cgcggccgcg gccggagacg
     2281 gtcatcgagc cggcggtggc cggcacgatg atcgacgtga tgcaggacgt ggtgcgccgg
     2341 ggcacgggca cggcggcgcg cattcgcggc tgggagccgg cgggcaagac cggcaccacg
     2401 cagaacaacg ccgacgcgtg gttcatcggc accgtcccgg tgatgtccac ggccgtgtgg
     2461 gtgggccacc cggacgccca gatcccgatg cgcggcgtga cgggcggctc actgacggcg
     2521 cgcatctggc agcgcttcat ggcgcaggcg ctggagggcg tggagccggt gccgttcccg
     2581 gagacgccgc cgctgtccag cgtgctgggc aagggcaaca cgcagattcc ggacgtggtc
     2641 ggcatggagg ccaccgaggc gctgcgcacc ctggcgcggg cggggctggt cggcaccgtg
     2701 cgcagcgtgg acaccacgcg cacgcccggc tcggtgctgt ggcagagccc gcgcgacggg
     2761 cgcacggccc gccgcggctc gaccgtgacc
//
LOCUS       DATMKC010000134        17463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_351749, whole genome shotgun sequence.
ACCESSION   DATMKC010000134 DATMKC010000000
VERSION     DATMKC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 17463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17463
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351749"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNU01_03975"
     CDS             <1..210
                     /locus_tag="VNU01_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31809.1"
                     /translation="AAFYLNAPGFKQVAQKVFRSEDGREFMVNSMVLNFDHRKLTGKR
                     HLVAVGLLASYPLWLKLGRRLGARA"
     gene            complement(229..645)
                     /locus_tag="VNU01_03980"
     CDS             complement(229..645)
                     /locus_tag="VNU01_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31810.1"
                     /translation="MRRYLVVANRTLPGPHLSQFVTDCLGAGTCHFDIVVPATPLPGH
                     WVSDVDEEHRMARERLEETLAAFRALGASAEGWVTDPHPIAAVSDALRGRDYDEIVVS
                     TLPPGASRWLRMDLPSRLERLTAVPVTHLVAEPAHA"
     gene            complement(718..1182)
                     /locus_tag="VNU01_03985"
     CDS             complement(718..1182)
                     /locus_tag="VNU01_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWH31811.1"
                     /translation="MPRDLPVTQVMSRDVLTVRPQDSLEDVVRLLAGRNISGAPVVDA
                     DGMLTGLLDDSDLLVADARLHAPTVVELFGAYLTMPGEQRRYEEELRHALARTVGDVM
                     RTPSPSVTDEALVEDVATIMVEKGVSRVPVIDHTGRVVGIVTRGDLVAAMGR"
     gene            complement(1226..2956)
                     /locus_tag="VNU01_03990"
     CDS             complement(1226..2956)
                     /locus_tag="VNU01_03990"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWH31812.1"
                     /translation="MTSYAEFTEIDEFERLIADFRSGAVDEETFRTRRLHMGCYGIRQ
                     SDTHMIRVKVPGGTLAPEHLDALADVAERWSRGFGHITTRQNFQFHFVRLDDVPAVMR
                     RIAEAGMTTREACGNAIRNVTQSELAGIDPTELVDTRPAVETTVQYFLRHPAFQALPR
                     KFKIAFDGSSADRAQLGIHDVGALGTVGPDGTTGFQVFVGGGLGSTPREADLLEPFTP
                     ADRFLPTVEAILEVFNEHGERRNRVRARMKFLLNKWGIEKFREEVFTRRERLEAELSY
                     PPLVLPRREPAPVLSRRPEPTDVLDPEAAVAFRQWRDVNVFAHEGPSADDPRFGAFVT
                     FHLGDVTVDQFRMLAKVWRELGPQVELRATIRQNLLFTNLTAQDLSDLYAVLRDGGMD
                     LARAEKSGDVVSCPGAETCNLAITASRGLGDAVTDALVAAGLDEIENLRINISGCPNA
                     CGQHTSADLGFAGMARRDANGNEAPGYRIYVGARLDAGGVKFGHYVAKVPAKKAPEVA
                     VEILGRYAAERDSGESFADWVARITPDELKAALKPHDTMPAMDEDPSFYADWGMSRQF
                     EVILGRGECA"
     gene            3130..4440
                     /locus_tag="VNU01_03995"
     CDS             3130..4440
                     /locus_tag="VNU01_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HWH31813.1"
                     /translation="MDVWERIGGALEAVRGHSASPPGGRLGAALALLHDPGDGDLELV
                     FTRRRDDLRSHPGQISFPGGRVDPGETPEQAAVREAREEVALDPSTVTVLGRLPAFYI
                     PPSRFWLHAVLARWDAPHPLVPAEAEVAEVLRARVSALRDPSVWRSVELSSGGHTWAW
                     QLDERHLLWGATAVVTSVILSLIDPAWSGGMSPAHLDAERMVRPWEGAPAARPQAARL
                     HGVAELPLADAAVLPPGALRGVPEAAALAEASRAVAAAAQNLLAGRGGAGGVLVLVGR
                     GGTGAIGAAAAAALAAHGAAVTVVTAGDPGDLPEATRRHLDGLDARPFDGRLPDAALV
                     VDALLGGGLEERVREPALGPVLALRHLAVPVVSVDVPSGVHPDGGLVGEAVTADVTVA
                     IGGVRPAACMAGIEPFVGDLYLASGGTPPLLRLVRPARRPGWRE"
     gene            complement(4450..6720)
                     /locus_tag="VNU01_04000"
     CDS             complement(4450..6720)
                     /locus_tag="VNU01_04000"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HWH31814.1"
                     /translation="MGRFRITAAGFALVLVAAFAVAEAWRPPVPAPDRGRGAGTGTGT
                     EVLDVTLASSLEPFDDCDAFLDTVRGEALAQVSAYGLGGGEQFMGGGAVAMREEAAMD
                     TAAGAPAAMGGEAGGGSTAGSEPMPMPQEMTQDDAGVVEVSGTNVQEGGVDEPDVVKT
                     DGRVLAAIARGRLQLVDLAGGARVAATVPLPDGWDHQLLLHGDRLLVTANVHEAMAPG
                     EPVDQPADPAASRPAEDRFATSDMAIMPAGAPKVDLVLYDVSDPGAPEQLGSVRLDGQ
                     LASSRLVDGVVRLVLRSQPQPALTLPQPGPEADAAQMRALESQAEERNREIVRTSQAA
                     DWLPGYAVRDAGGDTVREAPLVDCADVRHPEQFRGFGMLSVLALPLDGDGAQALAPTG
                     TASVLGDGDTVYATPDRLYVAMNRYEQAMTADIHEFDTSDPRRPVYTASGSVPGHLLN
                     QWALSFHEGHLRVAATTGQSGPDSADSESAVHVLRRDGARLVEVGRAGGLGKGERIYA
                     VRYFGDYGFVVTFRQTDPLYTLDLSDPAAPKVLGELKVPGFSSYLHPVGDGLLLGVGQ
                     DADESTGRRLGVQVSLFDVSDLAAPARVANLTLEPESSTDVEHDHRAFLWWPAARRAV
                     MPYMHARWDESAQREAGFFGALALHVDGGLREAGRISHAEPDARDPWWSQVRRALVVG
                     DTLWTMSERGLMASDVASLARRGFVEFDGPTPLDFTEDPGVIEPEPVDPPRREPAAER
                     DPGPGLDVVKELAPRG"
     gene            6871..7431
                     /locus_tag="VNU01_04005"
     CDS             6871..7431
                     /locus_tag="VNU01_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HWH31815.1"
                     /translation="MASVQVATRFFALLASACALFVVAAAALGLASAAGHERARRVRA
                     DVADALRAPAMALVAAIALVATGGSLYYSEIAGFPPCRLCWLQRGAMYPLAVAATAAA
                     AAGAAGWRRPLRVLALAGAAVSAWHVAVERWPALEGAACDPANPCSVVWVRDLGVFGI
                     PAMALAGFAAAAVLLSLRPPAHGGAS"
     gene            8045..8437
                     /gene="mscL"
                     /locus_tag="VNU01_04010"
     CDS             8045..8437
                     /gene="mscL"
                     /locus_tag="VNU01_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWH31816.1"
                     /translation="MLGEFKQFVLRGNVLDLAVGVVLGVAFNAVVQAFTDGVLMAAVA
                     ALFGEPNFDAIVWGLGDGELRVGALLTALVNLLLVGLALFAVVKAVNALRDRADREES
                     AEPAAKPAPSDEAVLLTEIRDLLRDGRR"
     gene            complement(8442..8516)
                     /locus_tag="VNU01_04015"
     tRNA            complement(8442..8516)
                     /locus_tag="VNU01_04015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8480..8482),aa:Leu,seq:taa)
     gene            complement(8539..10251)
                     /locus_tag="VNU01_04020"
     CDS             complement(8539..10251)
                     /locus_tag="VNU01_04020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWH31817.1"
                     /translation="MTGREIDAQPPAATGGGALAQRLRELRQASAAMRRVRWFGAAFV
                     AVQFTLYRPPAGMELPFPRYTVAAAVLAAVLAVNVASLWSRRVDSPRLLARIGAAELV
                     TDACIVLTVTWLFSFDTTSALWALLIIPVLEGAVRGQLRGAVSTWGLIAVGYLLRDLW
                     AASRYDYAPFLPESVTYRMFIVLIVAITSGLLARNLTRTAADLAVQVRAHREAQRESE
                     RRADLLQAVAAAGRHMTSLDLDTLLAIVVDSATGIGFDSAAICLLQPDGGFTVPHLSG
                     LPECDPEETFPSDRGIVGLALRRGETVAVTDPLPWFSLDQPVSFARTVAAPVWSGEEM
                     AAVLVAGLHGDMEIPLHVQECFELLAGQAGAALVNALRYRERRAFEAELQHQAYHDAL
                     TGLPNRAAFLEALTGSLAPLGPDEHGDGLVAVLFCDLDRFKTINDTLGHDFGDKLLAA
                     VAERLRQCVKEQDMVARLGGDEFTVLLGAIQDETQAEATARRVIHAMNRPLLLDGREV
                     YVTTSVGIAYGFRGRHSETDLLRAADLAMYQAKDEGRDRYAVSEASGALPDEFGLAAR
                     EVVL"
     gene            complement(10319..11341)
                     /gene="zapE"
                     /locus_tag="VNU01_04025"
     CDS             complement(10319..11341)
                     /gene="zapE"
                     /locus_tag="VNU01_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HWH31818.1"
                     /translation="MPIRLVDRHPDPPAAKLIAGLVPPPRFDAVRFGTYAPTHDSQHT
                     AAQRLESLAAELHRRAHGRGGVLGRLRRREDAPRSVYLDGGFGVGKTHLLASLWHASP
                     GPKTYATFGELVALVGALGMTAASEALRGTRLVCIDEFELEDPANTRLIAALLHRLVA
                     DGACVAATSNTLPGELGQGRFHAEHFKREIETLAAAFDTLTLHGQDYRGRGAPEPPPP
                     WPAERVLATARALGDRASLDDGVALLAHLATLHPIRYRDLVDGLDLVLVTDVRPVRDL
                     SGALRVAYLVDELYDQQIPVALSGAGGAELFAEPLLRGTYRKTLGRCVSRLGALQREA
                     EERAAA"
     gene            complement(11439..13136)
                     /locus_tag="VNU01_04030"
     CDS             complement(11439..13136)
                     /locus_tag="VNU01_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31819.1"
                     /translation="MDFFQEPPRLTNTYRADVPLREHLERLLPADVLAQVEPELDTVG
                     EDAAGGLLELARQAEREQPVHVPYDPWGRRVDEIRVSPAWEELHRYQAAHGMCAVPYE
                     GAFGEHARVVQHALIHVYSPSSAIYTCPMAMTDAAVRVLLDHAEPELRDRVVPRLTSR
                     EPDAWTSGQWMTEREGGSDVGRTATRARKGPDGRWKLYGTKWFTSATTADCALTLARP
                     EGAPEGSGALALFLVELVDPVTGESQIGKTIRVNRLKDKLGTKALPTAELTLDGAFVT
                     PIGDPWAGRGLKKISGMLNVTRAHNAMGAASGMRRGLDLALAYARVRESFGASLIDLP
                     LHRETMAELAVEAEASFALALRVMQLTGRVEHGVATEVEARALRGLLPVTKLLTGKDA
                     VAHASEALESFGGAGYIEDTGLPQLLRNAQVLPIWEGTTNVLSLDLLRATVKENALSA
                     LLEDLVARLEDADAPALADTLRLVAAERDALAAQALAWAQTDPELVQAGMRRFALRLG
                     RAYTAALLVEHAAYRLAKHNDDRAAIVARRYAERWLDPSHAPAPTADHAADTARLLT"
     gene            13193..14341
                     /locus_tag="VNU01_04035"
     CDS             13193..14341
                     /locus_tag="VNU01_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWH31820.1"
                     /translation="MTAEEERIALFLDYENLAIGARDDLGGFKFDLKPLADALAERGR
                     VVVRRAYADWSYFDDDRRMLTQQHVELIEIPQRMGTVRKNAADIKMAVDAIELSFERD
                     YITTFVICTGDSDFTPLVGKLRELNKRVIGVGLEASTSKLLPPACDEFLFYERLEGVN
                     APSSRRGSGGGRKSRGGRGGDRSRGGKVQQAAPVPVDELEEIEELDEQDAPQEEKNTD
                     PAELQKLVTQTLSGLQRSTGGAVLASTLKRALLRKDPTFSEADYGFRAFGELLRHLEE
                     RKIIELTVGSAKGDPEVSFPEHSSDEEGAFELLREVVADLEADAPPPLSGLKDQLRKR
                     RPEFSEKEFGFGGFLQFCKAARARGIVRMEWDEDVDDYVLRTQDAGTD"
     gene            14488..15129
                     /locus_tag="VNU01_04040"
     CDS             14488..15129
                     /locus_tag="VNU01_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH31821.1"
                     /translation="MSGETIRVVIVDDHALVREGLRSLLGQYGDIAVVGEAGTVDDAL
                     ETVETLRPDLVLLDLRLDGEDGAEVARRLRATGSKVRILVLSVHDTSRHLREALAAGA
                     DGYLLKSVSAASLADGIRGAAAGQTVIGQEFVPKLLEDASRGVPMGEPELTPREQQVL
                     ELVAEGLANREIAEKLGISTRTAQKHLENLFKKVGVHDRTELVTRAFRQGMLG"
     gene            15273..15611
                     /locus_tag="VNU01_04045"
     CDS             15273..15611
                     /locus_tag="VNU01_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWH31822.1"
                     /translation="MKLVVAIVKPFKVDDVKEALREAGVAGLTVTDARGFGRQRGHTE
                     VYRGAEYQVDFVPKSRIEVMVDDAEVDGVIDAIVKAARTGKIGDGKIAVLPIEEVVRI
                     RTGETGAEAL"
     gene            15631..>17463
                     /locus_tag="VNU01_04050"
     CDS             15631..>17463
                     /locus_tag="VNU01_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWH31823.1"
                     /translation="MRPADVTAPAGPQWARAWTALLDAGLSASLEAVGQAAGGVALVA
                     LGSYARGEVCPSSDVDLLLLHDGRPQRDLEGVVRTVCYPLWDGGLSVGHAVRTPKDAV
                     RAAGERIDTSTALVDRRLVAGDIGLADELGERAGRWLRKAGPGLLERLAAADAERHAR
                     AGSHAGMLEPDLKDGAGGLRDLQSLRWAAACLLGDATLDALVGARYLGAADRRELAEA
                     NAVLLATRCALHLVRDGGRPGRPGGDADRLRLDTQDEVAAALGDADGDALLHRVNLAM
                     RVVAHVHGRAWPLLLSDARGGRRRRKAPARRAGDGIAVLDGFVELDPEARLSDDPSLP
                     LRACAVAAAEGTHLGRASAMRLRRELEGHPGLPWDEPGRRALLDLLRQGPRALPVLAD
                     ADHVGLLNALLPEWERVRGLPQRNPFHRFALDLHGFQAVAELTRVAAGELDERHARLW
                     RELPDPDVLLLAALLHDVGKAWDGDHSVVGAGVAARWLAHMGFDSGRIATVATLVRHH
                     LLLPDTATRRDLDDEDEIAGVAAAVGSVEVLDGLYLLSLADARATGPSAWSAWKDQLM
                     AQLHRRARRVLDGRAARTDTPETLLEEARRRLGGGTELREEELQE"
BASE COUNT         2176 a         6351 c         6651 g         2285 t
ORIGIN      
        1 gcggcgttct acctgaacgc gcccgggttc aagcaggtgg cgcagaaggt gttccgctcc
       61 gaggacggcc gcgagttcat ggtgaactcg atggtgctca acttcgacca ccgcaagctg
      121 accggcaagc gccacctcgt cgcggtgggc ctgctcgcca gctacccgct gtggctcaag
      181 ctcggccggc gcctcggcgc ccgcgcctga gggcgctcgc ggcggggctc aggcgtgcgc
      241 cggttcggcg acgaggtgcg tcaccggcac ggccgtcagg cgctccagcc gcgatggcag
      301 gtccatgcgc agccagcgtg aggccccggg tggcagcgtg gacaccacga tctcgtcgta
      361 gtcgcggccg cggagcgcgt cgctgaccgc cgcgatggga tgggggtccg tcacccagcc
      421 ctcggccgag gcgcccaggg cgcggaaggc ggcgagagtc tcctccaggc gctcgcgcgc
      481 catgcggtgc tcttcgtcca cgtcgctgac ccagtgtccc ggcagcggcg tcgcggggac
      541 gacgatgtcg aagtggcagg taccggctcc caggcagtcc gtgacgaact ggctcaggtg
      601 cgggccgggc agagtccggt tggcgaccac gaggtagcgt cgcatctgac gcctccttgt
      661 cggtggggat cccaccgtac gcccgcgcgg tcccggcgtg aagatcgaac ggcggtgtca
      721 gcggcccatg gcggcgacca ggtcgccgcg cgtgacgatg cccacgacgc ggccggtgtg
      781 gtcgatgacg gggacccgcg acaccccctt ctcgaccatg atcgtcgcga cgtcctccac
      841 cagcgcctcg tcggtcaccg acggcgaggg cgtgcgcatc acgtccccca cggtgcgcgc
      901 cagcgcgtgg cgcagttcct cctcgtagcg ccgctgctcg cccggcatcg tgaggtaggc
      961 gccgaacagt tccaccacgg tgggcgcgtg caggcgggcg tcggcgacca gcaggtcgct
     1021 gtcgtccagc agtcccgtga gcatgccgtc ggcgtccacc accggggcgc cgctgatgtt
     1081 gcggccggcg agcagccgca cgacatcctc caggctgtcc tgcgggcgca cggtgaggac
     1141 gtcgcggctc atgacttggg tgacgggaag gtctctgggc atgactgcat cctaggcctc
     1201 cgcccctgct gcgggtcggg aacgctcagg cgcactcgcc gcggccgagg atgacctcga
     1261 actggcggct catgccccag tcggcgtaga agctcgggtc ctcgtccatc gcgggcatgg
     1321 tgtcgtgggg cttcagcgcc gccttcagct cgtccggcgt gatgcgggcc acccagtcgg
     1381 cgaaggactc gccggagtcg cgctcggcgg cgtaacggcc gaggatctcc accgccacct
     1441 cgggggcctt cttggccggc accttggcga cgtagtggcc gaacttcacc ccgcccgcgt
     1501 ccaggcgggc gcccacgtag atgcggtagc cgggcgcctc gttgccgttg gcgtcgcggc
     1561 gcgccatgcc ggcgaagccg aggtcggccg aggtgtgctg cccgcaggcg ttggggcagc
     1621 cggagatgtt gatgcgcagg ttctcgatct cgtccaggcc cgcggcgacc agcgcgtcgg
     1681 tgaccgcgtc gcccagcccg cgcgaggcgg tgatcgccag gttgcaggtc tccgcgccgg
     1741 ggcagctcac cacgtcgccg gacttctcgg cgcgcgccag gtccatgccg ccgtcgcgca
     1801 gcaccgcgta caggtccgac aggtcctgcg cggtcaggtt ggtgaacagc aggttctggc
     1861 ggatggtcgc gcgcagctcc acctgcggac ccagctcgcg ccacaccttg gcgagcatgc
     1921 ggaactggtc cacggtcacg tcgccgaggt ggaacgtcac gaaggcgccg aagcgggggt
     1981 cgtccgcgga cgggccctcg tgcgcgaaca cgttcacgtc ccgccactgg cggaacgcca
     2041 ccgccgcctc cgggtccagc acgtcggtgg gctccggccg ccgggacagc accggcgccg
     2101 gctcgcgccg cggcagcacc agcggcgggt aggacagctc ggcctccagg cgctcgcggc
     2161 gcgtgaagac ctcctcccgg aacttttcga tgccccactt gttgagcagg aacttcatcc
     2221 gggcgcgcac gcggttgcgg cgctcgccgt gctcgttgaa gacctccagg atcgcctcca
     2281 ccgtcggcag gaagcggtcg gcgggcgtga acggctccag caggtccgcc tcccgcggcg
     2341 tggagcccag gccgcccccg acgaacacct ggaagccggt ggtgccgtcc ggccccacgg
     2401 tgcccagcgc gccgacgtcg tggatgccga gctgcgcgcg gtccgcggag gagccgtcga
     2461 aggcgatctt gaacttgcgc ggcagcgcct ggaacgcggg gtggcgcagg aagtactgca
     2521 ccgtggtctc cacggcgggg cgcgtgtcca ccagctccgt ggggtcgatg ccggccagct
     2581 cgctctgcgt cacgttgcgg atcgcgttgc cgcaggcctc gcgcgtggtc atgccggcct
     2641 cggcgatgcg gcgcatcacc gccggcacgt cgtccagccg cacgaagtgg aactggaagt
     2701 tctgccgcgt ggtgatgtgg ccgaagccgc ggctccagcg ctcggccacg tccgccagcg
     2761 cgtcgaggtg ctcgggggcg agcgtgcccc cgggcacctt cacgcggatc atgtgcgtgt
     2821 cggactggcg gatgccgtag cagcccatgt gcagccgccg cgtgcggaag gtctcctcgt
     2881 ccacggcgcc cgagcggaag tccgcgatca ggcgctcgaa ctcgtcgatc tcggtgaact
     2941 cggcgtagga ggtcatgggt cctcactggg ggttcctggg tgccgtgcgg ctgtccacga
     3001 ggatgggcgc cggccgtgcg ggcggcaacg gacagcggca gggaaaacac acaaaacccc
     3061 ccgtcgggac agtgaaaacc cgtcccggcg ggcgagggac ctccgcgccg ggtcgggtag
     3121 ggtgcggccg tggacgtgtg ggagcggatc ggcggcgcgc tcgaggcggt gcgcgggcac
     3181 agcgcgtccc cgccgggcgg gcggctgggc gccgcgctgg cgctgctgca cgaccccggc
     3241 gacggcgacc tggagctggt gttcacccgc cgccgcgacg acctgcgcag ccaccccggc
     3301 cagatctcct tcccgggcgg gcgcgtcgac ccgggtgaga cgccggagca ggccgccgtg
     3361 cgggaggcgc gggaggaggt ggcgctcgac ccgtccaccg tgacggtgct cggccgcctg
     3421 cccgcgttct acatcccgcc atcgcgcttc tggctgcacg ccgtgctggc gcgctgggac
     3481 gccccccacc cgctggtccc cgccgaggcc gaggtcgccg aggtgctgcg cgcgcgcgtg
     3541 tcggccctgc gcgacccgtc cgtgtggcgc tccgtggagc tgtccagcgg cgggcacacc
     3601 tgggcctggc agctcgacga gcgccacctg ctgtggggcg ccaccgccgt ggtgacgtcg
     3661 gtgatcctaa gcctgatcga cccggcctgg tccggcggca tgagcccggc gcacctggac
     3721 gccgagcgca tggtgcgccc gtgggagggc gcgccggcgg cgcgcccgca ggcggcgcgc
     3781 ctgcacggcg tggcggagct gccgctcgcc gacgccgcgg tgctgccgcc gggcgccctg
     3841 cgcggtgtgc cggaggccgc cgccctggcg gaggcgtcac gcgccgtggc ggccgcggcg
     3901 cagaacctgc tggcgggccg cggcggggcg ggcggcgtgc tggtcctggt cggccggggc
     3961 ggcacgggcg ccatcggggc ggccgccgcc gcggcgcttg ccgcgcacgg cgcggccgtc
     4021 accgtggtga cggccggaga ccccggggac ctgccggagg cgacccggcg gcacctggac
     4081 ggcctggacg cgcggccctt cgacgggcgc ctgccggacg ccgcgctggt cgtggacgcg
     4141 ctgctgggcg gcggcctgga ggagcgggtc cgcgagccgg ccctcgggcc ggtcctggcg
     4201 ctgcgccacc ttgccgtgcc cgtcgtcagc gtcgacgtgc ccagcggcgt gcacccggac
     4261 ggcgggctgg tcggcgaggc ggtcaccgcg gatgtcaccg tggcgatcgg cggcgtccgg
     4321 ccggccgcgt gcatggccgg catcgagccg ttcgtgggcg acctgtacct ggcgtcgggc
     4381 gggacgccgc cgctgctgcg gctggtgcgc ccggcgcgcc gccccggctg gcgggagtga
     4441 ctccgggcgt cagccgcgcg gggccagttc cttcaccacg tccaggccgg ggcccgggtc
     4501 ccgctcggcc gcgggctcgc gccgcggcgg gtcgaccggc tcgggctcga tcacgcccgg
     4561 gtcctcggtg aagtccagcg gcgtggggcc gtcgaactcc acgaagccgc gccgcgccag
     4621 gctggcgacg tcgctggcca tgagcccgcg ctcggacatg gtccacaggg tgtcgcccac
     4681 cacgagggcg cggcgcacct gcgaccacca cgggtcgcgc gcgtccggct cggcgtggct
     4741 gatgcggccg gcctcgcgca ggccgccgtc gacgtgcagc gccagggcgc cgaagaagcc
     4801 ggcctcgcgc tgcgcggact cgtcccagcg ggcgtgcatg tacggcatga cggcgcgccg
     4861 agcggccggc caccacagga acgcgcggtg gtcgtgctcc acgtcggtgg acgactccgg
     4921 ctccagcgtg aggttcgcca cccgcgcggg cgcggccagg tcggacacgt cgaacagcga
     4981 cacctgcacg cccaggcggc ggccggtgga ctcgtcggcg tcctggccca cccccagcag
     5041 cagcccgtcg ccgacggggt ggaggtagga ggagaagccg ggcaccttga gctcgccgag
     5101 caccttgggg gcggccgggt ccgacaggtc cagcgtgtac agggggtcgg tctggcggaa
     5161 cgtcaccacg aagccgtagt cgccgaagta gcgcacggcg tagatgcgct cgcccttgcc
     5221 caggcccccg gcgcggccga cctcgaccag ccgcgcgccg tcgcgccgca ggacgtgcac
     5281 ggcgctctcg ctgtcggcgc tgtccgggcc ggactggccg gtggtcgccg ccacacgcag
     5341 gtggccctcg tggaacgaca gcgcccactg gttgagcagg tgcccgggca cgctgcccga
     5401 ggcggtgtag acgggccggc gcgggtcgct ggtgtcgaac tcgtggatgt cggcggtcat
     5461 cgcctgctcg tagcggttca tcgccacgta cagccggtcc ggtgtggcgt acaccgtgtc
     5521 gccgtcgccc aggacgctgg ccgtgccggt gggggcgagg gcttgcgcgc cgtcgccgtc
     5581 caggggcagg gccagcacgg acagcatgcc gaagccgcgg aactgctcgg ggtggcgcac
     5641 gtcggcgcag tccaccagcg gcgcctcgcg cacggtgtcg cccccggcgt cgcgcacggc
     5701 gtagcccggc agccagtcgg ccgcctgcga ggtccgcacg atctcgcggt tgcgctcctc
     5761 ggcctgcgac tccagcgcgc gcatctgcgc ggcgtcggcc tccgggccgg gctgggggag
     5821 cgtcagcgcc ggctggggct gggagcgcag caccaggcgc accacgccgt cgacgaggcg
     5881 ggaggacgcg agctggccgt ccaggcgcac actgccgagc tgctcgggcg cacccgggtc
     5941 ggagacgtcg tacaggacca ggtccacctt gggcgcgccc gcaggcatga tcgccatgtc
     6001 gctggtcgcg aagcggtcct cggcggggcg cgacgccgcc gggtcggcag gctggtcgac
     6061 cggttcgccc ggcgccatgg cctcgtggac gttcgcggtc accagcagcc ggtcgccgtg
     6121 cagcagcagc tggtggtccc agccgtccgg cagcggcacg gtcgcggcga cgcgcgcgcc
     6181 gccggccagg tcgacgagct ggaggcggcc gcgcgcgatc gcggccagga cccgcccgtc
     6241 ggtcttcacc acgtccggct cgtcgacgcc gccctcctgg acgttggtgc cggagacctc
     6301 caccacgccc gcgtcgtcct gggtcatctc ctggggcatc ggcatcggct cggagccagc
     6361 cgtggagccg ccgccggctt cgccgcccat cgcggcgggc gcgcccgccg ccgtgtccat
     6421 ggccgcctcc tcgcgcatgg ccacggcgcc cccgcccatg aactgctcgc cgccgcccag
     6481 gccgtaggcg ctcacctgcg ccagcgcctc gccgcgcacg gtgtccagga acgcgtcgca
     6541 gtcgtcgaac ggctccagcg acgaggccag cgtcacgtcc agcacctcgg tgccggtgcc
     6601 ggtgccggcg ccgcggccgc gatcgggcgc gggcacgggt gggcgccacg cctcggccac
     6661 ggcgaacgcc gcgaccagca ccagcgcgaa ccctgcggcg gtgatccgga aacggcccat
     6721 ctcgttcctc ctcgcggccg ggtcatgtca cggccatggt ggtgtgacgc cgcaccgcac
     6781 ccggcggttc cacggttgtg ggccggaagt cccgcaatgc ccgggccctg cgcgggtggg
     6841 ccccgcgcgc cgcgggcgga ccatgggctc atggcatcgg tccaggtggc gacgcgcttc
     6901 ttcgccctgc tggcatcggc ctgcgcgctg ttcgtcgtcg ccgcggcggc gctggggctc
     6961 gcctccgccg cggggcacga gcgcgcccgc cgcgttcgcg ccgacgtggc cgacgcgctg
     7021 cgggcaccgg ccatggccct cgtggcggcg atcgcgctcg tcgccaccgg cggcagcctg
     7081 tactactcgg agatcgcggg ctttccgccg tgccggctgt gctggctgca gcgcggggcg
     7141 atgtaccccc tggcggtcgc ggcgacggcc gcggcagcgg ccggcgcggc cggatggcgg
     7201 cggccgctgc gcgtgctcgc gctggccggt gccgccgtgt ccgcatggca cgtcgccgtg
     7261 gagcgctggc ccgcgctgga gggcgcggcc tgcgatccgg ccaacccgtg ctcggtcgtc
     7321 tgggtgcgcg acctgggcgt gttcggcatt ccggccatgg cgctggccgg cttcgccgcc
     7381 gccgccgtcc tgctgtcgct tcgaccgccc gcccacggag gtgcgtcatg accctcgccc
     7441 ccctcgctgc ccggcgactg tccgttccgt ggctggtcgc cggcctgctc ctcttcgcgg
     7501 gactcgtggc cctgctcacc gccggcggcg accccgcggg gccatcacag ttcggccccg
     7561 tgacggtcga gggggccccg ctgcccgcgg acggctcggc cggcatgccc gccccggcgc
     7621 tcgtgggggt cgacgtgcac cgcgacccgg tggaggtccg gcccgggcgg ccgacgctgc
     7681 tggtcttcgt ggcgcactgg tgcgcccact gccaggccga ggtgccccgc gtggtgcgct
     7741 ggcaccggga cggcggtggc ggagggctgc ggctggtggc cgtgtccacg gcgcagagcg
     7801 cgagcaagcc caactggccc gccacccggt ggctggaggc cgagggctgg ccgggcgcgc
     7861 tgctcctgga cgacgaggcg gggacggctg cgcgggcctt cggcgtgacg gcgttacccg
     7921 ttcttcgtgc tgctcgacgc ggacggcatc gtcctcgcgc gggcgtccgg cgagctgagc
     7981 ccggagtcgc tgcacgcgct ggacgggctt gtctgagcct gcggcgcggt gcacgcttgc
     8041 cggcatgctc ggcgagttca agcagttcgt gctgcgcggc aacgtgctcg acctggccgt
     8101 cggcgtggtg ctgggtgtgg cgttcaacgc ggtggtgcag gcgttcaccg acggcgtgct
     8161 gatggccgcg gtcgccgcac tgttcggtga gccgaacttc gacgcgatcg tctggggcct
     8221 gggcgacggc gagctgcgcg tcggcgcgct gctcaccgca ctggtgaacc tgctgctggt
     8281 gggcctcgcg ctgttcgccg tggtcaaggc ggtgaacgcg ctgcgtgatc gcgccgaccg
     8341 ggaggagtcc gcggagcccg cggccaagcc ggcgcccagc gacgaggccg tgctgctcac
     8401 cgagatccgc gacctgctcc gcgacggccg ccgctgagac gtgggggcgg tgggattcga
     8461 acccacacct gtcgccgttt taagcggcgt gcctctgcca gttgggccac acccccgtgg
     8521 tccaggccgc cagcgtagct agagcacgac ctcgcgggcg gccagcccga actcgtcggg
     8581 cagcgcgccg gacgcctccg acaccgcgta gcggtcacgc ccctcgtcct tggcctggta
     8641 catcgccagg tccgccgcgc gcagcaggtc cgtctccgag tggcgcccgc ggaagccgta
     8701 ggcgatgccg acgctggtcg tgacgtacac ctcccggccg tccagcagca gcggccggtt
     8761 catcgcgtgg atgacccggc gcgccgtcgc ctcggcctgc gtctcgtcct ggatggcgcc
     8821 cagcagcacc gtgaactcgt cgccgccgag gcgcgcgacc atgtcctgct ccttgacgca
     8881 ctggcgcagg cgctcggcca cggcggccag cagcttgtcg ccgaagtcgt ggccgagcgt
     8941 gtcgttgatg gtcttgaagc ggtccaggtc gcagaacagc accgcgacca ggccatcgcc
     9001 gtgctcgtcc gggcccagcg gggcgagcga gccggtgagg gcctccagga acgccgcgcg
     9061 gttcggcagg ccggtcaggg cgtcgtggta ggcctggtgc tgcagctccg cctcgaaggc
     9121 gcggcgctcg cggtagcgca gcgcgttgac cagggcggcg ccggcctggc cggcgagcag
     9181 ctcgaagcac tcctgcacgt gcagggggat ctccatgtcg ccgtgcagcc ccgccacgag
     9241 cacggccgcc atctcctcgc cggaccacac cggcgcggcg acggtgcgcg cgaacgacac
     9301 cggctggtcc aggctgaacc acggcagcgg gtcggtgacc gccacggtct ccccgcgccg
     9361 cagcgccagg ccgacgatgc cccggtcgga cgggaaggtc tcctccgggt cgcactccgg
     9421 caggccggac aggtgcggca cggtgaagcc gccgtccggc tggagcaggc agatggcggc
     9481 gctgtcgaag ccgatgccgg tggcggagtc cacgacgatg gccagcagcg tgtccaggtc
     9541 cagcgaggtc atgtgccggc cggcggccgc caccgcctgc agcaggtcgg cccggcgctc
     9601 cgactcgcgc tgcgcctcgc ggtgcgcgcg gacctgcacg gccagatcgg cggcggtgcg
     9661 ggtgaggttg cgcgcgagca ggccggaggt gatcgccacg atcagcacga tgaacatccg
     9721 gtaggtcacc gactccggca ggaacggcgc gtagtcgtag cggctggccg cccacaggtc
     9781 gcgcagcaga tacccgacgg cgatcaggcc ccaggtcgac acggcgccgc gcagctggcc
     9841 gcgcaccgcg ccttccagca ccgggatgat cagcagcgcc cacagcgccg acgtggtgtc
     9901 gaaggagaac agccacgtca cggtgagcac gatgcaggcg tccgtgacca gctcggcggc
     9961 gccgatgcgc gccagcagcc gtggcgagtc gacgcgccgg ctccacaggg atgccacgtt
    10021 cacggccagc accgccgcca ggaccgcggc ggcgaccgtg tagcgcggga agggcagttc
    10081 catcccggcc ggcggccggt acagggtgaa ctgcaccgcc acgaacgcgg cgccgaacca
    10141 gcgcacgcgc cgcatggcgg cggacgcctg gcgcagctcg cgcaggcgct gcgcgagagc
    10201 gccgccgcct gtcgcggccg gcggctgcgc gtcgatttcc cgaccggtca tagggactgg
    10261 tatcggcgcg cggggcgcgg gactagagca ttccgcccat ctccgtctac gtacctgctc
    10321 aggcggctgc gcgctcctcc gcctcgcgct gcagcgcgcc cagccggctc acgcagcggc
    10381 ccagcgtctt gcggtaggtg ccgcgcagga gcggctcggc gaagagctcc gcccccccgg
    10441 caccggacag cgccacgggg atctgctggt cgtacagctc gtcgaccagg tacgcgacgc
    10501 gcagcgcgcc ggacaggtcg cgcaccgggc gcacgtccgt gaccagcacc aggtccaggc
    10561 cgtcgacgag gtcgcggtag cggatcgggt gcagcgtggc gaggtgggcc agcagggcca
    10621 cgccgtcgtc caggctggcg cggtcgccca gcgcgcgcgc cgtcgccagc acgcgctcgg
    10681 ccggccacgg cggcggcggc tccggcgcgc cgcggccgcg gtagtcctgc ccgtgcagcg
    10741 tgagcgtgtc gaacgccgcg gccagcgtct ctatctcgcg cttgaagtgc tcggcgtgga
    10801 agcggccctg tcccagctcg ccgggcagcg tgttggaggt ggccgccacg caggcgccgt
    10861 cggcgaccag gcggtgcagc agcgcggcga tgagccgggt gttggccggg tcctccagct
    10921 cgaactcgtc gatgcacacc agccgggtcc cgcgcagcgc ctcggaggcg gccgtcatgc
    10981 cgagcgcccc caccagggcg accagctcgc cgaacgtcgc gtaggtcttc gggcccgggc
    11041 tggcgtgcca cagtgacgcc agcaggtgcg tcttgccgac gccgaagccg ccgtccaggt
    11101 agaccgagcg cggcgcgtcc tcccggcggc ggaggcgtcc caggacgccg ccgcggccgt
    11161 gcgcgcgccg gtgcagctct gccgccaggg actccaggcg ctgcgccgcg gtgtgctggc
    11221 tgtcgtgcgt gggggcgtag gtgccgaagc ggaccgcgtc gaagcgcggt ggcggcacca
    11281 ggccggcgat cagcttcgcg gcgggcggat cggggtggcg gtcgaccagg cggatgggca
    11341 cgaggccgga tggtaccggc gctcgctgat ccacagtttc acgccagaag cgggtgcggt
    11401 ttggcgcaga tacgcgccaa accgcacccg cctcggcgtc aggtgagcag gcgcgcggtg
    11461 tcggcggcgt ggtcggcggt gggggccggg gcgtgtgacg ggtccagcca acgttcggcg
    11521 tagcggcgcg ccacgatcgc ggcgcggtcg tcgttgtgct tggccagccg gtaggcggcg
    11581 tgctccacca ggagcgcggc cgtgtaggcg cgtcccagcc gcagcgcgaa ccggcgcatg
    11641 ccggcctgga ccagctccgg atccgtctgc gcccaggcca gcgcctgggc cgcgagggcg
    11701 tcgcgctcgg cggccaccag gcgcagggtg tccgcaagcg cgggcgcgtc ggcgtcctcc
    11761 aggcgcgcca ccagatcctc cagcagtgcc gacagcgcgt tctccttgac cgtggcgcgc
    11821 agcaggtcca gggacagcac gttggtggtg ccctcccaga tgggcaggac ctgggcgttg
    11881 cgcagcagct gcggcaggcc ggtgtcctcg atgtagccgg cgccgccgaa gctctccagc
    11941 gcctcgctgg cgtgcgccac cgcgtccttg ccggtcagca gcttggtcac gggcagcagg
    12001 ccgcgcagcg cgcgcgcctc cacctccgtg gccacgccgt gctccacacg gccggtgagc
    12061 tgcatgacgc gcagcgccag ggcgaacgac gcctcggcct ccacggccag ttccgccatc
    12121 gtctcgcggt gcagcggcag gtcgatgagg ctggcgccga aggattcgcg cacgcgcgca
    12181 tacgccaggg cgaggtccag gccgcggcgc atccccgagg ccgcgcccat cgcgttgtgc
    12241 gcgcgcgtga cgttgagcat gccgctgatc ttcttcaggc cccgcccggc ccacgggtcg
    12301 ccgatcggcg tcacgaacgc gccgtccagc gtgagctccg cggtcggcag cgccttggtg
    12361 cccagcttgt ccttgaggcg gttcacgcgg atcgtcttgc cgatctggct ctcgcccgtg
    12421 acggggtcga ccagctccac caggaacagc gccagcgcgc cgctgccctc cggcgcccct
    12481 tccggccgcg ccagcgtcag cgcgcagtcc gccgtggtcg ccgacgtgaa ccacttggtg
    12541 ccgtacagct tccagcgccc gtccgggccc ttgcgcgcgc gggtcgcggt gcggccgacg
    12601 tcgctgccgc cctcccgctc ggtcatccac tgcccggacg tccaggcgtc gggctcgcgc
    12661 gaggtcaggc gcggcaccac gcggtcccgc agctcgggct cggcgtggtc cagcaggacg
    12721 cgcaccgcgg cgtccgtcat ggccatcggg caggtgtaga tcgccgagga cggcgagtag
    12781 acgtggatca gcgcgtgctg caccacccgc gcatgctcgc cgaaggcgcc ctcgtagggc
    12841 acggcgcaca tgccgtgcgc ggcctggtag cggtgcaact cctcccacgc cgggctgacg
    12901 cggatctcgt cgacgcggcg gccccacggg tcgtagggca cgtggaccgg ctgctcccgc
    12961 tcggcctggc gggccagctc cagcagcccg cccgcggcgt cctcgccgac ggtgtcgagc
    13021 tccggttcca cctgcgccag cacgtcggcc ggcagcagcc gttccaggtg ctcgcgcagc
    13081 gggacgtcgg cccggtaggt gttggtcaag cgcggaggtt cctggaagaa gtccatgtgg
    13141 gcaatcttgt cgtgcgacag gtccccgcgt caaaggtcta gagtcggatc ccatgaccgc
    13201 agaagaagaa cgcatcgccc tgttcctgga ctacgagaac ctcgccatcg gcgcacgaga
    13261 cgacctcggc gggttcaagt tcgacctcaa gcccctggcc gacgcgctcg ccgagcgcgg
    13321 ccgggtggtg gtgcgccgcg cgtacgcgga ctggtcctac ttcgacgacg accgccgcat
    13381 gctcacccag cagcacgtgg agctgatcga gatcccgcag cgcatgggga cggtccgcaa
    13441 gaacgccgcc gacatcaaga tggccgtcga cgcgatcgag ctcagcttcg agcgcgacta
    13501 catcaccacg ttcgtgatct gcaccggcga cagcgacttc acgccgctgg tcggcaagct
    13561 gcgcgagctg aacaagcgcg tgatcggcgt ggggctggag gcctcgacct ccaagctgct
    13621 gccgcccgcg tgcgacgagt tcctgttcta cgagcgcctg gagggcgtga acgcgccgtc
    13681 cagccgccgg ggctcgggcg gcgggcgcaa gtcccgcggc ggccgcggcg gggaccgctc
    13741 gcgcggcggc aaggtgcagc aggccgcgcc cgtgccggtg gacgagctgg aggagatcga
    13801 ggagctcgac gagcaggacg cgccgcagga ggagaagaac accgaccctg cggagctgca
    13861 gaagctcgtg acgcagaccc tgtcgggcct gcagcgctcg accggcggcg cggtgctcgc
    13921 ctccacgctc aagcgcgcgc tgctgcgcaa ggacccgacg ttctccgagg cggactacgg
    13981 cttccgcgcg ttcggcgagc tgctgcgcca cctggaggag cgcaagatca tcgagctgac
    14041 cgtcggctcc gccaagggcg acccggaggt cagcttcccg gagcactcca gcgacgagga
    14101 aggggccttc gagctgctgc gcgaggtggt cgcggacctg gaggccgacg cgccgccgcc
    14161 gctgtcgggc ttgaaggacc agctgcgcaa gcgccgcccg gagttcagcg agaaggagtt
    14221 cggcttcggc ggcttcctgc agttctgcaa ggccgctcgt gcccgcggca tcgtgcgcat
    14281 ggaatgggac gaggatgtcg atgactacgt cctgcgcacc caggatgccg gcacggattg
    14341 attggaacgg aagcgacgtg tgcctcctcg ctattactca caggagcgtg cagaatgcgc
    14401 cgagttacct gactctcggt caaaccgccc gggtggggcg tcggaactgg cgaagcgagc
    14461 atggtgcttg aggcagcaga ggaactgatg tcgggggaga cgatccgggt cgtcatcgtc
    14521 gacgaccacg cgctggtccg cgagggtctg cgcagcctgc tcggccagta cggcgacatc
    14581 gccgtggtgg gcgaggccgg cacggtcgac gacgcgctgg agacggtcga gaccctgcgg
    14641 ccggacctgg tgctgctcga cctgcggctg gacggcgagg acggcgccga ggtcgcccgc
    14701 cgcctgcgcg ccaccggctc caaggtgcgc atcctcgtcc tgagcgtgca cgacacctcc
    14761 cggcacctgc gcgaggcgct ggccgccggc gcggacggct acctgctcaa gagcgtcagc
    14821 gcggcgagcc tggccgacgg catccgcggc gccgcggccg gccagaccgt gatcggccag
    14881 gagttcgtgc ccaagctgct ggaggacgcc tcccgcggcg tgcccatggg cgagccggag
    14941 ctgaccccgc gcgagcagca ggtcctggag ctggtggccg agggcctggc gaaccgggag
    15001 atcgccgaga aactgggcat ctccacgcgc accgcccaga agcacctgga gaacctgttc
    15061 aagaaggtcg gcgtccacga ccgcacggaa ctcgtgaccc gcgccttccg gcagggcatg
    15121 ctcggctagg caggcgcctc gcgggcaggg ctgctcggcg cagcaggtgt aaccaaggcg
    15181 aaacaccggg ctgacgacgc agaaatctgc ggggtgcacg ctggagccat cgaatctggc
    15241 agcagcggcc gacgcgccgc gggagggacg tcatgaagct ggtcgtggcc atcgtcaagc
    15301 cgttcaaggt cgacgacgtc aaggaggccc tccgcgaggc cggtgtggcc gggctcacgg
    15361 tgaccgacgc gcgcggcttc ggccgccagc gcgggcacac ggaggtgtac cgcggcgcgg
    15421 agtaccaggt ggacttcgtg cccaagagtc gcatcgaggt gatggtcgac gacgccgagg
    15481 tcgatggcgt gatcgacgcc atcgtcaagg cggcgcgcac cggcaagatc ggcgacggca
    15541 agatcgccgt gctgccgatc gaggaggtcg tgcgcatccg caccggcgag acgggcgccg
    15601 aggcgctcta ggcgcccggc ctcccgcgcc atgcggcccg cggacgtcac cgcgcccgcg
    15661 ggcccgcagt gggcccgcgc gtggaccgcg ctgctcgacg ccgggttgtc ggcctcgctc
    15721 gaagccgtcg gccaggcggc cggcggcgtg gcgctggtgg cgctgggctc ctacgcccgc
    15781 ggcgaggtgt gcccgtcctc cgacgtcgac ctgctcctgc tgcacgacgg ccgtccgcag
    15841 cgcgacctgg agggcgtcgt ccggacggtc tgctacccgc tgtgggacgg cggcctgtcc
    15901 gtcgggcacg cggtgcgcac ccccaaggac gccgtgcgcg cggccggcga gcgcatcgac
    15961 acgtcgaccg cgctggtgga ccgcaggctc gtggccggcg acatcggcct ggccgacgag
    16021 ctgggcgagc gcgccggccg ctggctgcgc aaggccggtc ccgggctgct ggagcgcctg
    16081 gccgccgcgg acgccgaacg ccacgccagg gccggcagcc atgccggcat gctcgagccg
    16141 gacctcaagg acggcgccgg cggcctgcgc gacctgcaga gcctgcgctg ggccgcggcc
    16201 tgcctgctcg gcgacgcgac gctggacgcc ctggtcggcg cccgctacct gggcgcggcg
    16261 gaccggcgcg agctggccga ggcgaacgcc gtcctgctgg ccacccgctg cgcgctgcac
    16321 ctggtgcgcg atggcggccg gcccggccgg cccggcggcg acgccgaccg cctgcggctc
    16381 gacacgcagg acgaggtggc ggcggccctg ggtgacgccg acggcgacgc gctgctgcac
    16441 cgtgtcaacc tggcgatgcg cgtcgtcgcg cacgtgcacg gccgtgcctg gccgctgctg
    16501 ctgagcgacg cccgcggcgg ccgacggcgc cgcaaggccc cggcccggcg cgccggcgac
    16561 ggcatcgccg tactggacgg cttcgtggaa ctggacccgg aggcgcggct gagcgacgac
    16621 ccgtccctgc cgctgcgggc atgcgccgtc gcggcggccg agggcacgca cctcggccgg
    16681 gccagcgcca tgcggctgcg ccgcgagctg gagggccatc ccggcttgcc ctgggacgag
    16741 ccgggacggc gggcgctcct ggacctgctg cgccagggcc cgcgcgcgct cccggtcctg
    16801 gcggacgcag accacgtcgg cctgctcaac gcgctgctgc ccgagtggga gcgcgtgcgc
    16861 ggcctgccgc agcgcaaccc gttccaccgc ttcgcgctgg acctgcacgg cttccaggcc
    16921 gtcgccgagc tgacacgcgt ggccgcgggg gagctggacg agcgccacgc gcgcctgtgg
    16981 cgcgagctgc cggaccccga cgtgctgttg ctggccgccc tgctgcacga cgtgggcaag
    17041 gcgtgggacg gcgaccacag cgtcgtgggc gcgggggtgg cggcccgctg gctggcgcac
    17101 atgggcttcg acagcgggcg catcgccacg gtggccacgc tggtgcgcca ccacctgctg
    17161 ctgcccgaca ccgcgacgcg ccgcgacctg gacgacgagg acgagatcgc gggggtggcc
    17221 gccgccgtgg gcagcgtgga ggtgctggac gggctgtacc tgctgtcgct ggcggacgcg
    17281 cgcgcgaccg ggccgtccgc ctggtcggcg tggaaggacc agctcatggc gcagttgcac
    17341 cgccgcgcca ggcgggtgct ggacgggcgc gcggcgcgca cggacacacc cgagacgctg
    17401 ctggaggagg cgcgccgccg gctgggcggc ggcacggagc tgcgcgagga ggagctccag
    17461 gag
//
LOCUS       DATMKC010000135         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_268096, whole genome shotgun sequence.
ACCESSION   DATMKC010000135 DATMKC010000000
VERSION     DATMKC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268096"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VNU01_04055"
     CDS             <1..925
                     /locus_tag="VNU01_04055"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HWH31824.1"
                     /translation="CAHDRHAGDRPARQAGAVTGAPLRTAVLGPGRVGTALAMALPPE
                     LYRVVAVAGRGRAALARFLAAVPGAAERGAVDAAREADFVLLAVPDDALVPLVRDLAA
                     AGAVRAGAAWVHTAGGHGLAALEPARLAGARVAACHPAQTFPDPETGRASLPGTAWAV
                     TAAAGDLGWARSLVHALGGVPVEVAEANRALYHAGLVVGANGASSVVAQARELLLGAG
                     VPDPEAFLAPLVVAAVRNAAGRGVAALTGPVRRGDTGTLQRHVAELRRAMPEAVEAYL
                     ALARLALAQARRAGLEPARADAVEALLREES"
     gene            925..1776
                     /gene="panC"
                     /locus_tag="VNU01_04060"
     CDS             925..1776
                     /gene="panC"
                     /locus_tag="VNU01_04060"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWH31825.1"
                     /translation="MLLVEEPEELRATLETLRCAGRRVGFVPTMGALHEGHLSLVRAA
                     RADCDVVVVSIFVNPLQFGAGEDLDRYPRQLEADLALLEPLAADVVFHPAAERFTPPG
                     RLTSVRVAGITEVLEGAARPGHFDGVSTIVTKLFNAVGPCRAYFGEKDFQQLAVVRRM
                     AADLNQPVEVIGCPIVRDADGLALSSRNVYLSPEQRRDALALSAALREVAGKWRGDAG
                     WARTYLANTLGDAPNVRLDYAEVVDPVTLEPLEGVVRGEARALVAAYVGDTRLIDNIR
                     LEPPTGA"
     gene            1807..2190
                     /gene="panD"
                     /locus_tag="VNU01_04065"
     CDS             1807..2190
                     /gene="panD"
                     /locus_tag="VNU01_04065"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWH31826.1"
                     /translation="MKSKIHRATVTAANLHYIGSITLDPELMEVANLLPNEKVQVVDI
                     DNGARLETYVISGVRGSGDVCLNGAAARLVQPGDKIIVISYGIFDDAEAREHEPVVVF
                     CDEDNEPILGVAERAHTTVEDLLGA"
     gene            2187..>2807
                     /gene="nadC"
                     /locus_tag="VNU01_04070"
     CDS             2187..>2807
                     /gene="nadC"
                     /locus_tag="VNU01_04070"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWH31827.1"
                     /translation="MSEQLAALVALALHEDLEHGGDVTATATIPADARGRARVVARRP
                     GVLSGLVAARAVVAAVDPSVAVEELASDGDALEPGTPVLELDGATRSLLAAERTMLNF
                     LGHLSGVATETARYVEELAGTRCALRDTRKTTPAMRELEKAAVRAGGGTNHRMGLYDG
                     ILVKDNHVAACGGVFEATRQALGYVAETGRDVEVQVEVDDVDQLREA"
BASE COUNT          333 a          981 c         1142 g          351 t
ORIGIN      
        1 gtgtgcgcat gatcggcacg caggtgaccg gcccgcacgg caggccggcg cggtgaccgg
       61 ggcgccgctg cgcaccgcgg tgctcggccc cgggcgggtg ggcacggccc tggccatggc
      121 gctgccgccg gagctgtacc gggtggtcgc ggtggcgggg cgcgggcgcg cggcgctggc
      181 gcgcttcctg gccgccgtgc cgggcgccgc ggagcgcggc gcggtggacg ccgcgcgcga
      241 ggcggacttc gtgctgctgg cggtgcccga cgacgcgctg gtgccgctgg tgcgggatct
      301 ggcggccgcc ggcgccgtgc gggccggcgc ggcgtgggtc cacacggccg gcggccacgg
      361 cctggccgcc ctggagccgg cgcgcctggc gggcgcgcgc gtggcggcgt gccaccccgc
      421 gcagaccttc ccggacccgg agaccggccg cgcgtccctg cccgggaccg cctgggcggt
      481 cacggcggcg gccggcgact tgggctgggc gcgcagcctg gtgcacgcgc tgggcggggt
      541 gccggtggag gtggccgagg cgaaccgggc gctgtaccac gcggggctcg tcgtcggcgc
      601 caacggcgcc tcctcggtgg tggcgcaggc gcgcgagctc ctgctcggcg ccggcgtgcc
      661 ggaccccgag gcgttcctgg cgccgctggt cgtggcggcc gtgcgcaacg cggccggccg
      721 cggcgtggcg gcgctgaccg ggccggtgcg ccgcggcgac accggcacgc tccagcggca
      781 cgtcgcggag ctgcgccggg cgatgccgga ggcggtcgag gcctacctgg cgctggcgcg
      841 cctggcgctg gcgcaggcgc ggcgcgcggg tctggagccg gcgcgggcgg acgccgtcga
      901 ggcgctgctg cgggaggaga gctgatgctg ctggtcgagg agcccgagga gctgcgcgcc
      961 acattggaga cgctgcggtg cgccggccgg cgcgtcggct tcgtgcccac gatgggcgcg
     1021 ctgcacgagg ggcacctgtc cctggtgcgc gccgcgcggg ccgactgcga cgtcgtggtg
     1081 gtgagcatct tcgtgaaccc gctgcagttc ggcgccggcg aggacctgga ccgctacccg
     1141 cggcagcttg aggcggacct ggcgctgctg gagccgctgg ccgcggacgt ggtgttccat
     1201 ccggccgccg agcgcttcac accgcccggc cggctgacgt cggtgcgggt ggcgggcatc
     1261 acggaggtgc tggagggggc cgcgcgcccc ggccacttcg acggcgtgag caccatcgtc
     1321 accaagctgt tcaacgccgt ggggccgtgc cgcgcgtact tcggcgagaa ggacttccag
     1381 cagctcgccg tggtgcgccg gatggccgcg gacctgaacc agcccgtgga ggtgatcggc
     1441 tgcccgatcg tgcgcgacgc ggacgggctg gcgctgtcca gccgcaacgt ctacctgtcg
     1501 cccgagcagc gccgcgacgc gctggcgctg tcggccgcgt tgcgcgaggt cgccggaaag
     1561 tggcgcggcg acgcgggttg ggcaaggacg tatctggcga atacactggg tgacgcgccg
     1621 aatgtccggc tcgactatgc ggaggtcgtg gaccccgtga ccctcgaacc actggagggc
     1681 gtcgtgcggg gcgaggcgcg ggcgctggtc gccgcgtacg tgggtgacac gcgtttgatc
     1741 gacaacatcc ggttggagcc gccgacaggg gcctgacacg ggagcgaagc ggtgcagcgt
     1801 cacctgatga agtccaagat ccaccgggcc acggtcacgg cggcgaacct ccactacatc
     1861 gggtccatca ccctggaccc ggagctcatg gaggtcgcga acctgctgcc gaacgagaag
     1921 gtccaggtcg tcgacatcga caacggtgcc cgcctggaga cctacgtgat ctccggcgtg
     1981 cgcggctccg gcgacgtgtg cctgaacggc gcggcggccc gcctggtgca gcccggcgac
     2041 aagatcatcg tgatcagcta cgggatcttc gacgacgccg aggcgcgcga gcacgagccg
     2101 gtcgtggtgt tctgcgacga ggacaacgag cccatcctgg gcgtggccga gcgcgcgcac
     2161 acgacggtcg aggacctgct cggcgcgtga gcgagcagct cgccgccctg gtggcgctcg
     2221 ccctccacga ggacctcgag cacggcggcg acgtcacggc cacggccacc atcccagccg
     2281 acgcgcgcgg ccgcgcgcgc gtggtcgccc ggcgtcccgg cgtgctgagc ggcctcgtgg
     2341 ccgcgcgtgc cgtcgtggcc gccgtggatc cgtccgtggc ggtcgaggag ctcgcctccg
     2401 acggcgacgc gctggagccg ggcaccccgg tgctggagct ggacggcgcg acgcgcagcc
     2461 tgctggccgc cgagcgcacg atgctcaact tcctgggcca cctgtccggc gttgccacgg
     2521 agacggcgcg ctacgtcgag gagctggccg gcacgcgctg cgcgctgcgc gacacgcgca
     2581 agaccacccc ggcgatgcgc gagctggaga aggccgccgt gcgcgccggc ggtgggacca
     2641 accaccgcat gggcctctat gacgggatcc tggtgaagga caaccatgtc gccgcgtgcg
     2701 gcggcgtgtt cgaggccacc cgccaggcgc tgggctacgt ggccgagacg gggcgcgacg
     2761 tcgaggtgca ggtggaggtc gacgacgtcg accagctgcg cgaggcg
//
LOCUS       DATMKC010000136         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_172455, whole genome shotgun sequence.
ACCESSION   DATMKC010000136 DATMKC010000000
VERSION     DATMKC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172455"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..556)
                     /locus_tag="VNU01_04075"
     CDS             complement(179..556)
                     /locus_tag="VNU01_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31828.1"
                     /translation="MGLFDRFRRKRRHSPVPAPSAQLTRELVAFMRERDGVEAFIEPP
                     TAVWPMSLCLVDGDGDSVRRPVQDEDQARRLCNEQGVPLYDARIVGYPKRMKDFQRGV
                     KGERVSLDEMPPLDVVEDPDTDR"
     gene            638..1189
                     /locus_tag="VNU01_04080"
     CDS             638..1189
                     /locus_tag="VNU01_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWH31829.1"
                     /translation="MNECELRTRPMYRHEYEALGALGFFDDENVELLDGQVVLAAEEG
                     PDHGAVYRRLARILIEAVPAGEGEIGVGNPLSLSDLSSPQPDFMVVPPSDAYRAAHPT
                     TASFVVEVSNASRRVDLGFKAVLYAAAGIPEYWVVDLVRDEIVVHRDPAVKTWKSITR
                     HRDGVVRSLRHPNLAVDVRALLR"
     gene            complement(1198..>2215)
                     /locus_tag="VNU01_04085"
     CDS             complement(1198..>2215)
                     /locus_tag="VNU01_04085"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWH31830.1"
                     /translation="WKTVNRNKLPVTLNLADPRGAELCRELAKRSDVLVENFRPGTLE
                     RWGLGPDVLLADNPNLVVTRVSGFGQDGPYSGRPGFGTLAESLSGFAGTHGEEGGPPQ
                     LPAIALADEAAGVWGTMATLVALLHRERNGGGQVVDVSLYEPLFAMLGPLPALYDLTG
                     QEAPRLGNRMPFTAPRGAYRTADGKWLGVSGTSPAVAHRILAAVGRPELAEDPRFATN
                     EARLRHAEELDGIIAEWVGRHSLDDALAAFEAADAAAAPIYRMADVFADPHFAARGTI
                     ARVPDDDLGEVAMADVVPRLSRTPGRLRWAGKGMGAANEQVFNGLLGLDAAHLDQLRA
                     DGVI"
BASE COUNT          291 a          802 c          811 g          311 t
ORIGIN      
        1 acacgaacgg cagccagttc ttcatctgcc tggacgacgt gccgctgccg ccggcctacg
       61 ccgtgttcgg ccaggtcacg gccggcatgg acgtggtgga cgccatcgcg gcgctgccga
      121 cgcggggcga gacgcccgcc cagacggtgt tcgtggagtc ggtccgcgcc gcgtagggct
      181 agcggtcggt gtccggatcc tccacgacgt cgaggggcgg catctcgtcc agtgacacgc
      241 gctcgccctt gaccccccgc tggaagtcct tcatccgctt ggggtagccg acgatgcggg
      301 cgtcgtagag cggcacgccc tgctcgttgc agagccggcg ggcctgatcc tcgtcctgca
      361 ccggccggcg gacgctgtcg ccgtcgccat cgaccaggca caggctcatc ggccacacgg
      421 cggtgggcgg ctcgatgaac gcctcgacgc cgtcgcgctc gcgcatgaag gcgaccagct
      481 cgcgggtgag ctgggccgac ggtgcgggca cgggggagtg gcggcgcttg cgccggaagc
      541 ggtcgaacag gcccatggcc gccagtgtag gcacttgtcc acaagctgag cgggcccggc
      601 gttgtcgggc agccggctct cgacccatgc tgtcgccatg aacgaatgcg aactccggac
      661 ccgcccgatg taccggcacg agtacgaggc gctgggcgcc ctgggcttct tcgacgacga
      721 gaacgtggag ctcctcgacg ggcaggtggt gctggccgcc gaggagggcc ccgaccacgg
      781 cgcggtctac cgccggctcg cccgcatcct gatcgaggcc gtcccggccg gggagggcga
      841 gatcggagtc ggcaacccat tgagcttgag cgacctgtcc tcgccgcaac ccgacttcat
      901 ggtggtgccg cccagtgacg cctatcgcgc ggcgcacccg acgaccgcga gcttcgtggt
      961 cgaggtgtcc aatgccagcc gccgggtgga tctcggcttc aaggcggtgc tgtacgccgc
     1021 ggccggcatc cccgagtact gggtggtgga cctggtgcgc gacgagatcg tggtccatcg
     1081 cgacccggcg gtgaagacct ggaagtccat cacgcggcac cgcgacggcg tggtccgctc
     1141 acttcggcac ccgaacctgg cggtggacgt gcgggcgctg ctgcgctgac ttcaggctca
     1201 gatgacgccg tcggcgcgaa gctggtccag gtgcgcggcg tccagtccga gcagtccatt
     1261 gaacacctgc tcgttggccg cgcccatgcc cttgccggcc cagcgcagcc gtcccggcgt
     1321 gcgcgacagc cgcggcacca cgtccgccat cgcgacctcg cccaggtcgt cgtcgggcac
     1381 gcgcgcgatg gtgccgcgcg cggcgaagtg cgggtcggcg aacacgtcgg ccatgcggta
     1441 gatcggcgcc gccgcggcgt cggccgcctc gaacgccgcc agcgcgtcgt cgaggctgtg
     1501 gcggccgacc cactcggcga tgatcccgtc cagctcctcg gcgtggcgca ggcgcgcctc
     1561 gttggtggcg aagcgcgggt cctcggccag ctccggccgc ccgaccgccg ccaggatccg
     1621 gtgcgccacc gccggggagg tgccggagac gccgagccac ttgccgtcgg cggtgcggta
     1681 cgcgccgcgc ggcgccgtga acggcatgcg gttgcccagg cggggcgcct cctggccggt
     1741 gaggtcgtac agcgccggca gcggcccgag catcgcgaac agcggctcgt acaggctgac
     1801 gtcgacgacc tggccgccgc cgttgcgctc ccggtgcagc agcgcgacca gcgtagccat
     1861 cgtgccccac acgccggcgg cctcgtccgc gagcgcgatc gccgggagct gcggcgggcc
     1921 gccctcctcg ccgtgggttc cggcgaagcc ggacagggac tcggccagcg tgccgaaccc
     1981 gggccggccc gagtagggcc cgtcctggcc gaacccgctg acgcgcgtga cgaccaggtt
     2041 ggggttgtcc gcaagcagca cgtcgggccc gaggccccag cgctccagcg tgccgggccg
     2101 gaagttctcc accagcacgt cgctgcgctt ggccaactcg cggcacagct ccgcgccgcg
     2161 cgggtcggcc aggttcagcg tgaccggcag cttgttgcgg ttgacggtct tccac
//
LOCUS       DATMKC010000137         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_77910, whole genome shotgun sequence.
ACCESSION   DATMKC010000137 DATMKC010000000
VERSION     DATMKC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77910"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VNU01_04090"
     CDS             <1..519
                     /locus_tag="VNU01_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWH31831.1"
                     /translation="PELEDQARMWAESSNSQEAEDPVAAAERERDEYLDALRRERAEF
                     ENFRKRTTKERMDALDRGAEQLVAGLLSVLDSFGLALDAAGKSDDEQLAKGVELVHSQ
                     LLSTLEQAGLSQVEGVGAAFDPTIHEAMLQVEAADELGREVDEPVVVEVLRAGYRFKG
                     RVLRPAAVKVAR"
     gene            542..1699
                     /gene="dnaJ"
                     /locus_tag="VNU01_04095"
     CDS             542..1699
                     /gene="dnaJ"
                     /locus_tag="VNU01_04095"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWH31832.1"
                     /translation="MSQRDYFEKDYYATLGVARDASQAEIAKAYRKLARELHPDANPG
                     DKAAEERFKEVSEAYSVLSSTDKRAEYDEVRRLVSSGGFRGGFPGGGPGGGFRSGAGG
                     YTATGQGFEFDLGDLLGGIFGGGAAGGRRGAGGPRRGRDVETELTLSFSDALRGVTTT
                     LRLSGAAPCSTCAGSGARPGTAPVPCATCGGAGTIAQDQGLFSFSEPCPACGGSGRQI
                     PNPCANCKGSGVESRTREIRARIPAGVKDGATIRLKGKGEPGRNGAPAGDLLVKVHVQ
                     PHTLFGRRGDDLTIRVPITFAEAALGAKVSVPTLDGPVTVRIPGGTESGKTFRVRGKG
                     VERDGRRGDLLVSVDVAVPRKLTREQRKLLEQLADTQRGDDVRAHLAEVVQ"
     gene            1696..2106
                     /locus_tag="VNU01_04100"
     CDS             1696..2106
                     /locus_tag="VNU01_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH31833.1"
                     /translation="MSTHAVYIISVAAELAGVHPQTLRTYERRGLIRPARTAGGTRRY
                     SERDVERVRLIQELTQGEGVNLAGVLRILALQDRVDELRERLAEAERAIQELGREAER
                     AIDEHRRRTSAALVPYRPTQIERHPGVRGRSQGR"
     gene            2114..>3459
                     /locus_tag="VNU01_04105"
     CDS             2114..>3459
                     /locus_tag="VNU01_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWH31834.1"
                     /translation="MDLNRFTLKSSEALQAAAALATERGNPEIAPLHLLHALTGQAEG
                     VVYPVLAKLGAVPTALRSKAEEALRALPAAHGATSQPGVGKALLGVLNDAQERAKALG
                     DEYTSTEHLLLALSAEDGQAGKILREAGVAGDALLAALREVRGSQRVTSQTPEDTYQA
                     LERYARDLTKEARDGRLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGDPGVGKTAIVEG
                     LAQRIVAGDVPEGLKDKRLVSLDLGAMVAGAKYRGEFEERLKAVLTDIEAAEGEIVTF
                     VDELHTIVGAGKAEGSLDAGNMLKPMLARGALRMIGATTLEEYRDVEKDKALERRFQP
                     VHVGEPSVEDTVAILRGLKERYEVHHGVRITDSAIVAAARLSDRYLTNRFLPDKAIDL
                     IDEATSRLRIEIDSMPSEIDVLDRRAKQLEIEKAALSAESDDAAFSISSCLARRSST"
BASE COUNT          524 a         1231 c         1306 g          398 t
ORIGIN      
        1 cccgagttgg aggatcaggc ccgtatgtgg gccgaatcct ccaactcaca ggaagcggag
       61 gacccggtgg ccgcggccga gcgcgagcgg gacgagtatc tggacgccct gcgccgggag
      121 cgcgcggagt tcgagaactt ccgcaagcgc accaccaagg agcgcatgga cgccctggat
      181 cgcggcgccg agcagctcgt cgcgggcctg ctgagcgtcc tggacagctt cggcctggcg
      241 ctggacgccg ccggcaagtc cgacgacgag cagctcgcca agggcgtgga gctggtgcac
      301 agccagctct tgtccacgct cgagcaggcc ggcctgtccc aggtcgaggg cgtcggcgcc
      361 gccttcgacc cgaccatcca cgaggcgatg ctccaggtgg aggcggccga cgagctgggg
      421 cgcgaggtcg acgagccggt ggtcgtggag gtgctgcggg ccggttaccg cttcaagggg
      481 cgggtcctgc gccccgcggc cgtgaaggtg gcccgctgac gcggccgccg gaggtgcgtg
      541 catgagccag cgcgactact tcgagaagga ctactacgcg acgctcggcg tcgcgcggga
      601 cgcctcgcag gccgagatcg ccaaggcgta ccgcaagctg gcccgtgaac tgcaccccga
      661 cgccaacccc ggcgacaagg ccgccgagga gcgcttcaag gaagtcagcg aggcctactc
      721 ggtgctgtcc agcaccgaca agcgcgccga gtacgacgag gtgcgccggc tcgtgtcctc
      781 cgggggcttc cgcggtggct tcccgggcgg cggccccggc ggcggcttcc gctccggcgc
      841 cggcggctac accgccaccg ggcagggctt cgagttcgac ctgggcgacc tgctgggcgg
      901 catcttcggc ggcggcgccg ccggtggccg ccggggcgcc ggaggcccgc gccggggacg
      961 cgacgtcgag accgagctga ccctgtcgtt ctccgacgcc ctgcgcggcg tgaccaccac
     1021 gctgcgcctg tccggcgccg cgccgtgctc gacgtgcgcg ggcagcggcg cgcgccccgg
     1081 caccgcaccc gtgccgtgcg ccacgtgcgg cggcgcgggc accatcgccc aggaccaggg
     1141 cctgttcagc ttctccgagc cgtgccccgc ctgcggcggc agtggtcggc agatcccgaa
     1201 cccgtgcgcc aactgcaaag gcagcggggt ggagtcgcgc acgcgcgaga tccgggcgcg
     1261 catcccggcc ggcgtgaagg acggcgccac catccggctc aagggcaagg gcgagccggg
     1321 ccgcaacggc gcgccggccg gcgacctgct cgtgaaggtc cacgtccagc cgcacacgct
     1381 gttcggccgc aggggcgacg acctcaccat ccgcgtgccg atcacgttcg cggaggcggc
     1441 gctgggcgcg aaggtgtccg tgccgacgct ggacggcccg gtgacggtga ggatcccggg
     1501 cggcaccgag agcggcaaga cgttccgcgt gcgcggcaag ggcgtggagc gcgacggccg
     1561 ccgaggcgac ctgctggtct cggtcgacgt ggcggtgccg cgcaagctga cgcgcgagca
     1621 gcgcaagctg cttgagcagc tcgcggacac ccagcgcggc gacgacgtgc gcgcccacct
     1681 ggcggaggtc gtgcaatgag cacccacgcc gtctacatca tctccgtggc cgcggagctc
     1741 gcgggcgtgc acccgcagac gctgcggacc tacgagcgca ggggcctcat ccgcccggcc
     1801 cgcaccgccg gcgggacgcg ccgctacagc gagcgcgacg tggagcgcgt gcggctgatc
     1861 caggagctca cgcagggcga gggcgtcaac ctggccggcg tgctgcgcat cctggcgctg
     1921 caggaccgcg tggacgagct gcgggagcgg ctcgccgagg ccgaacgcgc catccaggag
     1981 ctgggccgcg aggcggagcg cgccatcgac gagcaccgcc ggcgcaccag cgccgcgctg
     2041 gtgccgtacc gccccacgca gatcgagcgg cacccgggcg tccggggccg atcccagggc
     2101 aggtgagcaa gtcatggacc tcaaccgttt cacgctgaag tcctccgagg cgctgcaggc
     2161 cgccgccgcg ctggccacgg agcgcggcaa cccggagatc gcgccgctgc acttgctgca
     2221 cgcgctcacc gggcaggccg agggggtggt gtaccccgtg ctggccaagc tgggcgccgt
     2281 cccgaccgcg ctgcgctcga aggcggagga ggcactgcgg gcgctgccgg ccgcgcatgg
     2341 cgccaccagc cagcccggcg tcggcaaggc gctgctgggc gtgctgaacg acgcgcagga
     2401 gcgcgccaag gcgctgggcg acgagtacac ctccaccgag cacctgctgc tggcgctgtc
     2461 cgcggaggac ggccaggccg gcaagatcct gcgcgaggcg ggcgtggccg gcgacgcgct
     2521 gctggcggcc ctgcgcgagg tgcgcggcag ccagcgcgtg accagccaga cgccggagga
     2581 cacctaccag gcgctggagc gctacgcgcg cgacctcacc aaggaggccc gcgacggcag
     2641 gctggacccg gtcatcggcc gcgacgagga gatccgccgg gtcatccagg tactgtcccg
     2701 gcgcaccaag aacaaccccg tgctgatcgg tgacccgggt gtcggcaaga ccgccatcgt
     2761 ggaggggctg gcgcagcgca tcgtggccgg cgacgtgccg gagggcttga aggacaagcg
     2821 cctggtgtcc ctggacctgg gggcgatggt ggccggcgcc aagtaccgcg gcgagttcga
     2881 ggagcggctc aaggccgtgc tcaccgacat cgaggcggcg gagggcgaga tcgtcacgtt
     2941 cgtcgacgag ctgcacacca tcgtgggcgc cggcaaggcg gaagggtccc tggacgccgg
     3001 caacatgctc aagcccatgc tggcgcgagg ggcattgcgc atgatcggcg cgaccacgct
     3061 ggaggagtac cgcgacgtcg agaaggacaa ggcgctggag cgacgcttcc agcccgtcca
     3121 cgtgggcgag ccgagcgtcg aggacacggt ggcgatcctg cgcgggctga aggagcgcta
     3181 cgaggtgcac cacggcgtgc gcatcaccga cagcgccatc gtcgccgcgg cccggctcag
     3241 cgaccgctac ctcaccaacc ggttcctgcc cgacaaggcc atcgatctca tcgacgaggc
     3301 gacctcccgc ctgcgcatcg agatcgactc gatgcccagc gagatcgacg tgctggaccg
     3361 gcgcgccaag cagctggaga tcgagaaggc ggcgctgtcg gccgagtccg acgacgccgc
     3421 cttctcgatc tccagctgct tggcgcgccg gtccagcac
//
LOCUS       DATMKC010000138         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_268840, whole genome shotgun sequence.
ACCESSION   DATMKC010000138 DATMKC010000000
VERSION     DATMKC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268840"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VNU01_04110"
     CDS             complement(<1..1239)
                     /locus_tag="VNU01_04110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWH31835.1"
                     /translation="MERGSELLRWDDVGLSGDGSPSPGTEEQFRLMFEQAPAGMALID
                     AHGRWMRVNAALCRLTGRTEQSMLALNLPALLHEDSELQAGRLAAFLSGRSLEYRDEL
                     RLRRASGDIVWTTLHAARIQAGGSADAFAIVQLEDVTERRAAEAMLTARALQDPLTGM
                     ANRVMLMERLRQALDTYETRVAVLFIDLDRFKVVNDTHGHEVGDRVLVEVAHRIQSNL
                     RSGDIAARLGGDEFIVLCTDVRSAADVTELVDRLLARVKEPIGANGQYVSVSASVGVV
                     VPEGGARPPEDLLRQADLAMYRAKQLGRARMIMFDDHLERHLERRLRLELGLHEAFRS
                     RQFEVWYQPLVDLRDGLVSGAEALVRWRHPARSSCRRATCWAGPCRRRSSRRCWPPSP
                     PRWRACGRTAPGRRVGAMDTD"
     gene            complement(1487..1573)
                     /locus_tag="VNU01_04115"
     tRNA            complement(1487..1573)
                     /locus_tag="VNU01_04115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1537..1539),aa:Leu,seq:cag)
     gene            1801..>2033
                     /locus_tag="VNU01_04120"
     CDS             1801..>2033
                     /locus_tag="VNU01_04120"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HWH31836.1"
                     /translation="MGILRDFERRLGGAVEGFFARAFRSGLQPVELAKALQRYAADYQ
                     QVGLDGVFIPNVYRFDLAPEDLDRFGSFRDMLE"
BASE COUNT          300 a          738 c          718 g          277 t
ORIGIN      
        1 gtcggtgtcc atggcaccga ctcgcctacc cggtgccgta cgcccgcatg cccgccagcg
       61 aggtgggctg ggcggccagc accgcctcga actccgccgc cggcatgggc cggcccagca
      121 ggtagccctg cgacagctcg accgtgccgg gtggcgccag cgcaccagcg cctccgcgcc
      181 gctgaccagc ccgtcgcgca agtcgacgag cggctggtac cagacctcga actgccggct
      241 gcggaacgcc tcgtgcaggc cgagctccag gcgcagccgc cgctccaggt gccgttccag
      301 gtggtcgtcg aacatgatca tgcgcgcgcg gccgagctgc ttggcgcggt acatcgccag
      361 gtcggcctgg cgcagcaggt cctccggcgg gcgggcaccg ccctccggca ccaccacgcc
      421 gacgctggcg gacacggaga cgtactggcc gttcgcgccg atcggctcct tgacgcgcgc
      481 cagcagccgg tccaccagct ccgtcacgtc cgcggcgctg cgcacatcgg tgcacaggac
      541 gatgaactcg tcaccaccga gacgggccgc gatgtcgccg gagcggaggt tggactggat
      601 gcggtgggcg acctcgacca ggacgcgatc gccgacctcg tgcccgtgcg tgtcgttcac
      661 gaccttgaag cggtccaggt cgatgaacag gacggccacg cgcgtctcgt aggtgtccag
      721 cgcctggcgc aggcgctcca tgagcatgac gcggttggcc atgccggtga gcgggtcctg
      781 cagggcgcgc gcggtcagca tcgcctccgc ggcccgccgc tccgtgacgt cctccagctg
      841 cacgatcgcg aaggcgtcgg cggagccgcc ggcctggatg cgcgccgcgt gcaaggtggt
      901 ccacacgatg tcgcccgagg cccggcgcag gcgcagctcg tcgcggtact ccagggatcg
      961 cccgctgagg aacgccgcca ggcgccccgc ctgcaactcg gagtcctcgt gcagcagcgc
     1021 ggggaggttg agcgcgagca tcgactgctc ggtgcggccc gtgagccggc acagcgcggc
     1081 gttgacacgc atccagcgcc cgtgcgcgtc gatcagcgcc atgccggccg gcgcctgctc
     1141 gaacatgagc cggaactgct cctcggtgcc cggggagggc gagccgtccc cggacaggcc
     1201 gacgtcgtcc catcgcagaa gttccgagcc ccgttccacg ccgcttctct tttccgtcgc
     1261 cggccgaaca tcggccgacg gacgaccgta ccgccctgtg ccccagggga acgagcgttg
     1321 gtcgcccgcc ttacggaaag agcgcaggct gaacggaaag ggtccggggt cgggcgtgta
     1381 ccaaagggca ggctgggccg tgtcaccggc gccggcgagg ttccgggacc actttcgtcc
     1441 gctcgaggac acaactcgtc ccgcgacggc ggtgaggtga ggggtgtggt gggcgagggg
     1501 ggacttgaac ccccacgtcc ttgcggacac aggaacctga atcctgcgcg tctgccaatt
     1561 ccgccactcg cccgagtgac atccgccgcc ccggggaagg gcaacggcgg cgaagtgtag
     1621 cagcgccgcc ggagggcggc atgtccagca ggcgcccgat ttgccccccg atcacggcgt
     1681 tgacgcgggg gctcacgcgt gtgaacgacc ggacaggagc gggcctgccg cgcagcgggt
     1741 acctccttct agactctcac ccacggccca cccccgacga cgagaggtgg caccaccagc
     1801 atgggcatcc tgcgggactt cgagcggcga ctcggcggcg cggtcgaggg cttcttcgcg
     1861 cgcgcgttcc gctccggcct gcagcccgtg gagctggcca aggcgctgca gcgctacgcc
     1921 gcggactacc agcaggtggg cctggacggg gtgttcatcc ccaacgtcta ccgcttcgac
     1981 ctggccccgg aggacctcga ccgcttcggc agcttccgcg acatgctgga gaa
//
LOCUS       DATMKC010000139         5096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_125228, whole genome shotgun sequence.
ACCESSION   DATMKC010000139 DATMKC010000000
VERSION     DATMKC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125228"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1459)
                     /locus_tag="VNU01_04125"
     CDS             complement(233..1459)
                     /locus_tag="VNU01_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31837.1"
                     /translation="MALAIALLAVGLPAAGHPGGSHGGSGGSGEHSHTDQPRHGLEEF
                     GFHPLDNKGFNTDIFPWASGDGRVWAATGTWGTALEPGRDCPSETDNPSDPAHSGIWI
                     IEATDPATPRLAAKLGTIPGAQNLDMKVVKVDAPDGPRDVLVHSLEPCGVEGLLHQVP
                     GTPLVDYAHTIQREAKLHQTGFQLYDVTDPDNPVRLGTFNNGGLGTHNLYPFHQGDRA
                     FVAAVYNEVDFVGLQNDRFIRGHVQIVEITDPAEPVLLSQWDLYDAAADGGPAEDALC
                     KERGVDSAFCYNHDVWVSDDGTILYNAFWDAGLVLLDISDPANPRFIGQAQEHTQADD
                     PEGWLNEEGNTHSMIPMELDGRHPSRSRRGAARCSRRSRTAPAPGSSRSARWTSAHRS
                     PRRRAPAGTRPAARSR"
     gene            1728..1800
                     /locus_tag="VNU01_04130"
     tRNA            1728..1800
                     /locus_tag="VNU01_04130"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1761..1763,aa:Ala,seq:ggc)
     gene            1970..2209
                     /locus_tag="VNU01_04135"
     CDS             1970..2209
                     /locus_tag="VNU01_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWH31838.1"
                     /translation="MTRLHRGDVANVRFADVVALMRWLGFDLVRVAGSHHVFAHPAVP
                     ELVNLQEVRGQAKPYQLRQVVALVQRYALSFEEER"
     gene            2206..2421
                     /locus_tag="VNU01_04140"
     CDS             2206..2421
                     /locus_tag="VNU01_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWH31839.1"
                     /translation="MTDYHINVFYSDEDGGYIADIPDLTACSAFGNTPEEALAEVLRA
                     KDAWLTAAREAGKPIPPPRYRAMPHAG"
     gene            2724..3065
                     /locus_tag="VNU01_04145"
     CDS             2724..3065
                     /locus_tag="VNU01_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31840.1"
                     /translation="MQYPDDLPEQPVALDGTILSVQREAYDEDAAGAPVTVELRVNEA
                     FRGDLGDTLTVRTWDFTNPGASPGPDPLGVRLLAAMTDSRDLMACGFTRPYSEQGAQA
                     WREAFRRERFE"
     gene            3369..4499
                     /locus_tag="VNU01_04150"
     CDS             3369..4499
                     /locus_tag="VNU01_04150"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HWH31841.1"
                     /translation="MAAEVTRRVLPDAERGCLLLADISGYTTYLTHTELEHAQDVLAD
                     LTTVITSSLEPVLHVVEVEGDAVFAYVLAGELDAGSLLDTIDTAYFWFRARARDVAQG
                     STCQCGACSLVPSLDLKFVVHDGRFVLQEIAGKRKPAGPDVVLAHRLLKNHVSEAFGL
                     TGYALLTQACTEALSIDPAALGLHPHVERYDDVGEVVCHIEDLQARWESEKQRRRVYV
                     LPADAQFSIERTLPATPVELWELSTEPSKRMLWHQGVTDINESNPGGRRGLGSTAHCL
                     HGSDVMVEEILDWQPYRYFTRRSVLPMIGPWAWTFEFAEVGDGQTQIRMRGEPLRGKA
                     RVAWTLMRPMMMRQLNHNFDLLEQVVVGGQRPANSRSTANTL"
     gene            complement(4457..4948)
                     /locus_tag="VNU01_04155"
     CDS             complement(4457..4948)
                     /locus_tag="VNU01_04155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HWH31842.1"
                     /translation="MALTALLSGPFALLAAMYENTMAGVAAVLLLAAVAPAIEETVKI
                     AGAAYVAEQRPWLVPTALVLPVVGLVSGLVFATVENWWYLTVLVPDPPRDLVAWRWVF
                     GPLVHGTGSFTAGIGVAVMWRRARESGHRPSFSDAQPWLVAAVVWHGAYNVFAVLLEF
                     AGR"
BASE COUNT          757 a         1814 c         1762 g          763 t
ORIGIN      
        1 tggagcacca cggccccgtc cggccagtca ggggacttct ggtacgccgg caccggctgc
       61 accccggccg actacgccat ggccgccgcc tacaacggct ccgggccgga gggcaagatc
      121 gtcttcgtcg actcgttcac gcccgagccc ccgttcgccc cgtgcggcgg ctacctgtac
      181 gcgcagaagg tcgccctggc cgaggccagt ggcgcggccg gcctggtgca gctcaccgac
      241 tgcgtgccgc tggacgagta cccgccggag cccgacgccg cggactgcgg tgagccgacg
      301 tccaccgcgc agacctcgac gatcccggcg ctggagctgt tcggctccga cgcgagcatc
      361 gcgctgcgcc gcgccgtgat cgactcgggt gccgcccgtc gagctccatc gggatcatcg
      421 agtgggtgtt gccctcctcg ttgagccacc cttccgggtc gtcggcctgg gtgtgctcct
      481 gggcctgccc gatgaagcgc gggttcgccg ggtcgctgat gtccagcagg accaggcccg
      541 cgtcccagaa ggcgttgtag aggatggtcc cgtcgtcgga gacccacacg tcgtggttgt
      601 agcagaacgc ggaatcgacg ccgcgctcct tgcagagcgc gtcctcggcg gggccgccgt
      661 cggccgccgc gtcgtagagg tcccactggc tgagcaggac cggctcggcg ggatcggtga
      721 tctcgacgat ctgcacgtgc ccgcggatga agcgatcgtt ctgcaggccg acgaagtcga
      781 cctcgttgta gaccgcggcg acgaacgccc ggtcgccctg gtggaacggg tacaagttgt
      841 gcgtgccgag gccgccgttg ttgaaggtgc ccaggcggac cgggttgtcg gggtcggtga
      901 cgtcgtagag ttggaagccg gtctggtgga gcttcgcctc gcgctggatg gtgtgcgcgt
      961 agtcgaccag cggcgtgccc gggacctggt gcagcaggcc ctccacaccg cacggctcca
     1021 ggctgtgcac caggacgtcg cgcgggccgt cgggcgcgtc gaccttcacg accttcatgt
     1081 cgaggttctg cgcgccgggg atggtgccga gcttggcggc cagccgcggg gttgcggggt
     1141 cggtggcttc gatgatccag atgccggagt gcgcggggtc gctggggttg tcggtctccg
     1201 acgggcagtc ccggccgggc tccagcgcgg tgccccaggt gcccgtcgcg gcccacacgc
     1261 gcccgtcgcc ggaggcccac gggaagatgt cggtgttgaa gcccttgttg tccagggggt
     1321 ggaaaccgaa ctcttccagg ccgtggcgag gctggtcggt gtgcgagtgc tcgccgctgc
     1381 cgccgctgcc gccgtggctg ccgcccggat gcccggcggc gggcagcccc accgcgagca
     1441 gcgcaatcgc cagcgccacc aagaccgggt gccgccgcgg gccccgccga ccacggattg
     1501 ccttcccacc attcgccatg ccgcgcgctc ccacgccggg cctggcgccc ccgccggtcc
     1561 tggctccccc ggacggcgca ccaggcgatc ccacctgttc ccatcgcgtt acccaccgtg
     1621 acaacgggtt gcacgtatcg ggcgtaaaaa cgccccgccc agcacggtcc ggcacggtcc
     1681 ggctggtgtg acacctccgg ggctcggtgg taccttcgtg acgcttcggg gctatagctc
     1741 agttggtaga gcgcttccat ggcatggaag aggtcagggg ttcgattccc cttagctcca
     1801 catttcgtgt caccgtacga acaccgttcc gcttggacgc cccggctttt ggccggggtg
     1861 tttgcgttcg gacgtgagcg acgcgtcaag cccacgactg cttgtggata gaccgactgc
     1921 gagaccagcc gcggcggtta gcgttcggcg catgcgtcct cgtgctctga tgacccgcct
     1981 gcacagaggc gatgtcgcca acgtgcggtt cgccgatgtg gtcgcgctga tgcggtggct
     2041 cggcttcgat ctcgtgcgtg tcgccggtag ccaccacgtc ttcgcccacc ccgctgttcc
     2101 cgaactggtg aacctgcagg aggtgcgcgg tcaggccaag ccctaccaac tccgccaagt
     2161 cgtcgcgctc gtccagcggt acgctctgtc attcgaggag gagcggtgac cgactatcac
     2221 atcaacgtct tctacagcga cgaggatggc ggctacatcg ccgacatccc cgacctcacc
     2281 gcctgctcgg cgtttggcaa cactcccgag gaggcgctcg cggaggtctt gcgcgccaag
     2341 gacgcctggc tcaccgctgc tcgcgaggca ggcaagccga tcccgccacc ccgctaccgg
     2401 gcgatgcccc acgccggttg aatccgccgc cggctcgctc gcgctcgaag cgagttcgaa
     2461 taagaacaag ccttctccac catccgcgac ctcatccgaa ccgcgtgcgc cccggttttt
     2521 ggccggggcg tctgcgccgg gacgttgccc aacggcgtgg cttcgtggac tcgtagtccg
     2581 acaggttcgg cgcgcgaggc gaagcgcgaa acgcccgcga acgcgccgac gcggcgtcga
     2641 ccaacacgaa tgcgcgacag gaagagccgc ccgattggct cgtctccatg tcggacgcgg
     2701 ctccgccggc cgccacttgt gccgtgcagt acccggacga cctgcccgag cagccggtcg
     2761 cgctcgacgg caccatcctg tcggtgcaac gcgaggcata cgacgaggac gccgccggcg
     2821 cgcccgtcac ggtagagctg cgcgtcaacg aggccttccg tggcgatctc ggtgacacac
     2881 tcacggttcg cacgtgggac ttcaccaacc ccggcgcttc acccgggcca gatccactcg
     2941 gcgtgcgcct gctcgccgcc atgaccgaca gccgcgacct catggcctgc ggtttcaccc
     3001 ggccctacag cgaacaaggc gctcaagcct ggcgcgaagc gttccggaga gagcggttcg
     3061 aataagaaca agccttctcc acgcacgcgg cctgcccttg gtcacgaggg caggccgctc
     3121 gcgttccagg cttgtcaccc cgcccttctc gggtgaggcg atgactcccg tcggaggctg
     3181 gcatgcccgg gccgtggggg ccggggggtg tcgcggcggg ccgcgccgta ctaggttccg
     3241 cgtgtgccgc cacagtgtcg gcatgacggc ctggcacccg cgacgggcaa tggggccagc
     3301 atcgagcgtg agacgcggac cgtcacgctc gcgtcgggcc ggcgaccacg atcgaggcga
     3361 ggatgtcgat ggcagccgaa gtgacacgac gagttctgcc ggacgcggag cgaggctgcc
     3421 tgctcctggc ggacatctcc ggctacacca cctacctcac acacaccgag ctggagcacg
     3481 cccaggacgt gctcgccgac ctgacgacgg tgatcaccag cagcctggag ccggtcctgc
     3541 acgtcgtgga ggtggagggc gacgcggtgt tcgcctacgt gctggccggc gagctggatg
     3601 ccggcagcct cctggacacc atcgacaccg cgtacttctg gttccgcgcg cgcgcacgcg
     3661 acgtcgcgca gggctccacc tgccagtgcg gggcctgctc gctcgtgccc tccctggatc
     3721 tcaagttcgt ggtccacgac ggccgcttcg tgctgcagga gatcgccggc aagcgcaagc
     3781 ccgcggggcc cgacgtggtg ctcgcgcacc ggttgctgaa gaaccacgtg tcggaggcct
     3841 tcggcctgac cggctacgcc ctgctcaccc aggcgtgcac ggaggcgctg tcgatcgacc
     3901 ccgccgccct tggcctgcac ccgcacgtcg agcgctacga cgacgtcggc gaggtcgtgt
     3961 gccacatcga ggacctgcag gcgcgctggg agtcggagaa gcagcgccgg cgcgtgtacg
     4021 tgctgcccgc cgacgcgcag ttcagcatcg agcgcaccct gccggccacc ccggtggagc
     4081 tgtgggagct gagcaccgaa cccagcaagc gcatgctgtg gcatcagggc gtcaccgaca
     4141 tcaacgagag caatcccggg ggacgccgcg ggcttggctc gacggcccac tgcctgcacg
     4201 gcagcgacgt gatggtggag gagatcctcg actggcagcc ctaccgctac ttcaccaggc
     4261 gatcggtgct gccgatgatc ggcccctggg cgtggacgtt cgagttcgcc gaggtgggcg
     4321 acgggcagac gcagatccgc atgcgcggcg agcccctgcg cggcaaggcg cgcgtggcct
     4381 ggacgctcat gcggccgatg atgatgcgcc agctgaacca caacttcgac ctgctcgaac
     4441 aggtcgtggt cggcggtcag cggccggcga actcgaggag caccgcgaac acgttgtagg
     4501 cgccgtgcca cacgacggcg gccaccagcc agggctgcgc gtcggagaag gacgggcggt
     4561 gaccgctctc ccgcgcacgc cgccacatca ccgcgacacc gatgcccgcg gtgaacgagc
     4621 ccgtgccgtg gaccagcgga ccgaacaccc agcgccacgc cacgaggtcg cgcggcgggt
     4681 cggggacgag cacggtgagg taccaccagt tctcgactgt cgcgaacacc agcccggaca
     4741 ccagcccgac gaccggcagc accagcgcgg tgggaaccag ccagggacgc tgctcggcca
     4801 cgtaggcggc gccggcgatc ttcacggtct cctcgatcgc cggggcgacg gccgcgagca
     4861 ggaggaccgc cgcgaccccg gccatcgtgt tctcgtacat ggcggcgagc agggcgaacg
     4921 ggccggacaa cagcgccgtg agcgccatgg ccgaccattt gcgcgcgcgc ccggtcgacg
     4981 cccaccgctc cgcgaacgcg tgcgcgcgca ccgggccgtc cgggcgccac ccgagcgccg
     5041 gctcggccga ctggtcgaag tcgccgttca ttcgtcagac ccgcacggcg gtgccg
//
LOCUS       DATMKC010000140        10502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_335802, whole genome shotgun sequence.
ACCESSION   DATMKC010000140 DATMKC010000000
VERSION     DATMKC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335802"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNU01_04160"
     CDS             <1..271
                     /locus_tag="VNU01_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31843.1"
                     /translation="GPGQGQFDADAFLAFAECMRANGVEDFPDPGADGRPNVEAMQGL
                     DRSSEEFQSASETCADEVGFEGPMGGGRGGFGGPGGPRGGPGGQA"
     gene            268..1398
                     /locus_tag="VNU01_04165"
     CDS             268..1398
                     /locus_tag="VNU01_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWH31844.1"
                     /translation="MKREATSSTLATGAPDATSSRRAPRWLWVLGVLALAGGVVVLIS
                     PGGRPSQASDAEGTATPRSTATVQRRDIVSTTSLSGTLTYAEARDVGAARGGTITWLP
                     EVGTTIEPGHPLFAVNGEPVVLLPGQLPMWRDLSVDSDPGADVEVLERALIDLGVTDE
                     ANLTVDETFTWVTAAAVADLREQVGLPEGDSIAVGEVVYLPGVSRVAGVHLEVGDLVQ
                     PGEAVLTATATARIVKVDLDAADQGLVAEGDTVEVELPDGSSVAGTVALVSPVAEART
                     STGQNATTTYVIPVEITLSSGGEAFDEAPVSITVASDVATDVLAVPVEALLALAEGGY
                     ALEVVQADGSTSLVAVTIGGFSDGWVAVEGADLSEGMEVVTA"
     gene            1395..2108
                     /locus_tag="VNU01_04170"
     CDS             1395..2108
                     /locus_tag="VNU01_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH31845.1"
                     /translation="MTAMPSTEQVISLRGVHKRYPGGVHALRGVDLDVNAGEMLAIVG
                     PSGSGKSTMLHIVGTLDLPTEGSVHLAGHDISHLNDRELSGLRARTIGFVFQQFFLLS
                     GTSALDNVATGAVYTGLPPRERRARAAAALDRVGLGHRVDHHPNELSGGERQRVAVAR
                     ALINDPQLILADEPTGNLDSKTEAQIIDLLQHLHDEGTGVLVITHSHEIAATMPRQVS
                     LHDGRIVADTAGRHVGAPA"
     gene            2105..3310
                     /locus_tag="VNU01_04175"
     CDS             2105..3310
                     /locus_tag="VNU01_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH31846.1"
                     /translation="MSATTPSTPLPTSVFRPGDIARAASGGLAANRLRAILSALGIAI
                     GIASMVAVLGIASSSRAELLATLDRLGTNLLAVTSGTSFVGDDVELPEMSTAMVERIG
                     PVEATASTTAVDAAVFRNELMPSGRTGGLAVHAADADLLHVLNGTLASGRFLDTAPPG
                     LPVTVLGATAAERLGGIRPGMQVVIDDERFGVIGVLEPLELVPALDSAAIVPRGVAET
                     LFGTSTAPSTLYVRTTPAQIDAVSGVLAATANPENPEAVEVSRPSDALAARDATESTF
                     TTLLLGLGAVALVVGGLGIANVMVIAVLERRSEIGLRRALGATRRFISRQFFGEAVLL
                     SGIGGLGGGLLGVGVVAVYATSRALPVAIDPGVPFAGVGAAVLIGAVAGVYPAVRAAR
                     MSPTEALNH"
     gene            complement(3563..4339)
                     /locus_tag="VNU01_04180"
     CDS             complement(3563..4339)
                     /locus_tag="VNU01_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWH31847.1"
                     /translation="MDVVVTERQGRIAVIRLDRPPVNAMDTGLLSSLILALEAYAESD
                     ARAAVLTADGGVFCAGVDLRMLLEGGPEYVREFLPLLSECFAALFTVPKPVIAAVNGH
                     ALAGGCILAAACDLRLMADGPGTIGVTELPVGVPFPSWALEIMRHATAPQHLGEVVLT
                     GRRYTPAEGLARGLLDEVVAPERLLDRALELAEDLARVPHATFAHTKRQLRHPAVERV
                     ERHGPDYNALAEGLWAAPEVTDAIRAYLEARVGRSGSRKD"
     gene            4532..4732
                     /locus_tag="VNU01_04185"
     CDS             4532..4732
                     /locus_tag="VNU01_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31848.1"
                     /translation="MLKNKLLIAMMAGLLSLGAVACADDAGDDAGTGTELEGDTGTDL
                     GTDTGTDTDLGTDTGTEGEVSE"
     gene            4886..5845
                     /locus_tag="VNU01_04190"
     CDS             4886..5845
                     /locus_tag="VNU01_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWH31849.1"
                     /translation="MTVRRRLFALSLLVVPALALAPAGAGPLPDNPWLERRVLNIAHQ
                     GGEIEAPSDTLYAFKTAVAKGVDMLELDVHATSDGHIVVLHDTTVNRTTDGAGRVDAL
                     TLKEIQRLDAAHWFVEGCGTCHGRPAADYTLRGIATHQRKAPRGFTWKDFRIPTLEEV
                     LKTFPDVPINIEIKNTAPDTPAYERTLADLLAEYGRDDDVVVVSFLDHAIEQFKLWAP
                     HVHTATATGETAAFWAGSQQALPGAPNPRYVAFQVPVTFNGVTVVTEDFVRDAHANGM
                     AVHVWTINDRAEMEWLLDIGVDGIMTDVPTVLEELLAERGQNS"
     gene            complement(5842..6273)
                     /locus_tag="VNU01_04195"
     CDS             complement(5842..6273)
                     /locus_tag="VNU01_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31850.1"
                     /translation="MTLPPDDPHAPPPHATPREVEAAGKVSEALEWVERARGRLYDLH
                     QMIGRADFLLGDAADLLDDAGHGGLAALLRRDVIGRNVVEGRWTFQLVEEFDATYYDS
                     VRDAERRVREELMDGRRHVHEALLKEARRTPGLRHHERRPG"
     gene            complement(6270..6971)
                     /locus_tag="VNU01_04200"
     CDS             complement(6270..6971)
                     /locus_tag="VNU01_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31851.1"
                     /translation="MKTRFRPEVPAPLAASLAGAFGGLSWLRHARVFHPRGVAFRGTL
                     RRTGLDPGTGSAFLDEPGEQLALVRFSKGAGLPDPLPDVLGIAVRALDADGPGRHRDL
                     LFASSAAPPVLRHLLVPARRLASRPCSSVLPYATPAGTVMLAAAAEDGPSVDRMAAGA
                     APAAVRLLAATTTGPWRTWAVLLPEHRLSAAEHAELAFDPWNAGVDLRPSGLLNRLRH
                     PAYAASRAARPGGGR"
     gene            7165..7956
                     /locus_tag="VNU01_04205"
     CDS             7165..7956
                     /locus_tag="VNU01_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWH31852.1"
                     /translation="MEAVNGRGQLLDAGIVALAGATLGQFAGELPLLLGLAGLSVLRL
                     AMGLRPRLGRARLRRRVPRLLRVVRLARPRRRRDADTGQLQLVLPEAPAVHDDAPELA
                     VIDALTGLPNRRGVERSLDDACRQAVGANQPLGVLVFQVRDAGQVRRLFGPQAARDVL
                     RQTGTRLSKALGDAGLVGRWAGEEFVAVVPGLRAEAAALLAERFRRVLEADPYRLSSG
                     RRVPVAMAAGWAASPRDGISGQFLVSRAQGRAAAARSDAGALALS"
     gene            8099..9955
                     /locus_tag="VNU01_04210"
     CDS             8099..9955
                     /locus_tag="VNU01_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWH31853.1"
                     /translation="MSIDEVLVERASIDEEIHGKTLLDLFERNAREHGDRPALNWMDA
                     QGQWQSATWREYRERAVRAAMGLRSLGVDPGDFVAIMAGNRPEHVIADVAAMYNGATA
                     VSLYNTLAPEQIVYVADNCGAKIAILENRDYFKRWEQIHDQLPRCEKVVLMEHADEFR
                     GYDWVLSWDEVMAAGEQALAAQPDAVEERRKTLTPETPATVIYTSGTTGPPKGVVLTH
                     RNILWELTATDHFNAFPDFPRGISYLPLAHVAERSFSHWNGINKAGSTYFCPEITRAM
                     EVVPVARPQAFVAVPRVWEKLHAGLLAALQAEPDERKRKIALKAVAVGQEVIERTQRG
                     DSVPLKLRLQHRLFDKLVYSKIREKIGLDACELALSGAAPIARDILVFFAGIGLPIHE
                     VYGMSETTAVTHGNPLGAIKIGTVGVPLPGVECRIAEDGEVLVRGGNMTPGYHNRPEA
                     TAELLDDEGWVHTGDLGTVDADGYLTIVGRKKELIITAGGKNISPNNIEGVLKEHPLI
                     GQVCCVGDMKPFISALIVLDGEAAPVWAAQHGVPFTDFASFSRDERVLSEVASAVEAA
                     NQRLAKVEQIKKWRVLEREWTPETEELTPTLKLKRNVIHDKYAADIESLYSS"
     gene            10114..>10502
                     /locus_tag="VNU01_04215"
     CDS             10114..>10502
                     /locus_tag="VNU01_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31854.1"
                     /translation="MQDMAQGIEYVNDVLFVGLAVVAFRQWRSRRDPQTAWVAVTFVL
                     LSAVVIGGLVLPESGEQPWRVWATKALLVGLLLFPWALYRFTVSFQGSRPRVETVARA
                     LTAGVLALTVLLPDVPAPAVSARATVST"
BASE COUNT         1484 a         3777 c         3836 g         1405 t
ORIGIN      
        1 ggggccgggg cagggccagt tcgacgccga cgcgttcctc gcattcgccg agtgcatgcg
       61 tgccaacggt gtcgaggact tcccggaccc tggggcagat gggcggccga acgtcgaagc
      121 gatgcagggt cttgatcgat ccagcgagga gttccagtcc gcgtcggaga cctgtgccga
      181 cgaggtgggc ttcgaggggc ccatgggtgg tggccggggc ggcttcggtg gaccgggggg
      241 tccccgcggt gggccgggag gtcaggcatg aagcgggaag ccacctcgtc gacgctggcc
      301 accggagcgc cggatgccac ctcgtcccgc cgcgcccctc gatggctgtg ggtgttgggc
      361 gtcctggcac tggcgggagg cgtggtcgtg ctgatctcgc cggggggccg tccttcgcaa
      421 gcatccgacg cggaggggac cgcgacgccg cggtcgaccg ccacggtcca gcggcgcgac
      481 atcgtgtcga cgacgtccct gtcaggcacc ttgacctatg cggaggcgcg tgacgtcggc
      541 gcggccaggg gtgggaccat cacctggctg ccggaggtcg gcaccaccat cgagccagga
      601 cacccgctgt tcgcggtcaa cggcgagccg gtcgtgctgc ttccaggtca actgccgatg
      661 tggcgggatc tgtccgtgga cagcgacccg ggtgcggatg tggaggttct cgagcgggcg
      721 ttgatcgatc tgggcgtgac ggacgaagcc aacctgaccg tggacgagac cttcacctgg
      781 gtgaccgcag cggccgttgc ggacctgcgt gagcaggtcg gcctgcccga aggtgacagc
      841 atcgcggtcg gcgaggtcgt ctacctgccg ggcgtgagcc gggtcgcggg tgtgcatctg
      901 gaggtcggcg acctggtgca gcccggggag gcggtcctga cggcgacggc gacagcgcgc
      961 atcgtgaagg tggatctgga cgcggccgac caaggtctgg tggccgaggg cgacacggtc
     1021 gaggtcgagc tgcctgacgg cagcagtgtg gccggcacgg tcgcgctcgt ctcgcctgtc
     1081 gccgaggcgc gcacgtcgac cggccagaac gccaccacca cgtatgtcat cccggtcgag
     1141 atcaccttgt cgagcggcgg cgaggcgttc gacgaggcgc ctgtgagcat caccgtggcc
     1201 tcggacgtgg ccaccgatgt cctggccgtg cccgtcgagg cgctcctggc gctggcagaa
     1261 ggcggctacg cgctcgaggt cgtgcaagcc gacggatcca cctcgctggt ggccgtcacg
     1321 atcggaggct tctcggacgg ctgggtcgct gtcgaaggcg ccgacctgag cgagggcatg
     1381 gaggtcgtga ccgcatgacg gcgatgccct ccaccgagca ggtcatctcg cttcgcggcg
     1441 tccacaagcg ataccccggc ggcgtccacg cgctgcgcgg cgtcgacctg gacgtcaacg
     1501 ccggcgagat gcttgccatc gtcgggccgt ccgggtcggg caagtccacg atgctgcaca
     1561 tcgtcgggac gctcgacctg ccgaccgagg gcagcgtgca cctcgccgga cacgacatct
     1621 cgcacctgaa cgatcgcgaa ctgtcgggtc tgcgcgcccg caccatcggg ttcgtcttcc
     1681 agcagttctt tctgctcagc ggcacgtcgg cgttggacaa cgtggcaacc ggcgccgtct
     1741 acacgggact gccaccgcgg gagcggcgcg ctcgggcggc agccgcgctg gaccgcgtcg
     1801 ggctcggaca ccgggtcgac caccacccca atgaactgtc cggaggcgag cgtcagcgag
     1861 tcgccgtcgc gcgcgcgctc atcaacgatc cccaactcat cctggccgac gaacccaccg
     1921 ggaacctcga ctccaagacc gaagcgcaga tcatcgacct gctgcagcac ctgcacgacg
     1981 aggggacagg cgtgctcgtg atcacgcact cgcacgagat cgccgcgacg atgccccggc
     2041 aggtgtccct gcacgacggc cgcatcgtgg cggacaccgc cggccgacac gtcggagcgc
     2101 cggcgtgagc gcaacgacgc cttcgacccc tctgccgacg tcggtgtttc gacccggcga
     2161 catcgccaga gctgcctccg gggggctggc cgccaaccgt ctgcgcgcga tcctgtccgc
     2221 cctcggtatc gcgatcggga tcgcgtcgat ggtcgcggtg ctcggcatcg cgtcgtcctc
     2281 gcgtgccgag ctccttgcga cacttgaccg gctcgggacc aacctgctcg cggtgacgtc
     2341 gggcaccagc ttcgtcggcg acgacgtcga gctgccagag atgtcgacgg cgatggtcga
     2401 gcgcatcggc cccgtcgagg cgaccgcctc gaccaccgcc gtcgacgccg ccgtgttccg
     2461 caacgagctg atgccgtcgg gccggacagg aggactcgcg gtccacgccg cggacgccga
     2521 cctgctccac gtcctgaacg gcacgctcgc gtccggccgg ttcctcgaca cggccccgcc
     2581 aggcctgccg gtgaccgtgc tcggcgcgac cgccgccgaa cgcctgggcg gcatccggcc
     2641 cggcatgcag gtcgtgatcg acgacgaacg cttcggcgtc atcggggtcc tcgaaccgct
     2701 cgagctcgtc ccagccctgg attcggcggc catcgtcccc cgcggcgtag ccgagacgct
     2761 gttcggcacc tccacagcac cctccacgct ctatgtccgg accaccccag cgcagatcga
     2821 cgccgtcagc ggtgtgctcg ccgcgaccgc caaccccgag aaccccgaag ccgtcgaggt
     2881 gtcacggccg tccgacgcgc tcgcggcacg ggacgcgacg gagagcacgt tcacgacact
     2941 gctgctcggg ctgggtgcgg tcgcgctcgt cgtcggaggc ctcggtatcg ccaacgtcat
     3001 ggtcatcgcg gtgctcgagc gccgcagcga gatcggactg cgtcgcgccc tcggtgcgac
     3061 acgccggttc atcagccgtc agttcttcgg agaggctgtg ctgctgtccg gcatcggcgg
     3121 cctcggaggc ggactcctcg gcgtcggcgt cgtggccgtc tacgcaacca gccgcgccct
     3181 ccccgtcgcg atcgacccgg gggtgccgtt cgccggcgta ggggcagcgg tcctcatcgg
     3241 tgccgtcgcc ggggtctacc ccgcggttcg ggccgcacgc atgagcccca ccgaggccct
     3301 caaccactag gacagtctga ccagagcgcg ccgcttggcc gggctgttgc accagcgggg
     3361 cgacccgtgg aaggccagag tcgtcgcgcc gctcagtcca ggcggagctt gcgggcgatg
     3421 atctcgtagc gtcgccacgc tgccctggac acggtcggtg ggcgtccgcg cggcggaggc
     3481 acgccccttc gctcttcccc gccagccccc cgacgacatg ctggtcttgg cccttgccgt
     3541 ccgtcatgcg cgccgaacgc agtcagtcct tccgcgaacc gctgcggccc acacgggcct
     3601 ccagataggc gcggatggcg tcggtcacct ccggcgccgc ccacagtccc tccgccagcg
     3661 cgttgtagtc cgggccgtgg cgctcgacgc gttcgaccgc ggggtgccgc agctgccgct
     3721 tggtgtgcgc gaacgtcgcg tggggcacgc gggccaggtc ctcggccagt tccagcgcgc
     3781 ggtcgagcag gcgctcgggc gcgaccacct cgtccagcag gcctcgcgcg agcccctcgg
     3841 ccggggtgta gcggcgcccg gtgagcacca cctcgccgag gtgctggggc gccgtcgcgt
     3901 ggcgcatgat ctccagcgcc caggacggga acggcacgcc gacggggagc tccgtcacgc
     3961 cgatcgtccc gggcccgtcc gccatgaggc gcaggtcgca ggcggccgcc aggatgcagc
     4021 cgccggccag cgcgtggccg ttcacggcgg cgatcacggg cttgggcacg gtgaacaggg
     4081 cggcgaagca ctccgacagc aggggcagga actcccgcac gtactccggg ccgccctcga
     4141 gcagcatgcg caggtccacg ccggcgcaga acacgccgcc gtcggccgtg agcacggcgg
     4201 cgcgcgcgtc ggactccgcg tacgcctcca gcgccaggat gaggctgctc agcaggcccg
     4261 tgtccatcgc gttcaccggc ggccggtcca gccggatgac cgcgatccgc ccctgccgtt
     4321 cggtgaccac cacgtccatc cgcacgcctc ctcgcccgct gccccgactc cggcgggcat
     4381 cgtacgggcc gcgcctgccc agccggacag gggccagccg atacgcgcaa gattcgtcgc
     4441 cgaaagcgcg ccggaagcag gattgacggg gtgtgaccgg ggtaggagga cgccgcaccc
     4501 cagtccgtct ctcgcggaag gaacctccca catgctcaag aacaagctgc tgatcgcgat
     4561 gatggccggc ctgctgtcgc tcggcgcggt cgcctgcgcc gatgacgccg gtgacgacgc
     4621 cggcaccggc accgagctcg agggcgacac cggcacggac ctcggcacgg acaccggcac
     4681 cgacacggac ctcggcaccg acaccggcac cgagggcgag gtctcggagt aggcgcccgc
     4741 cctccaaaga tcccgcacca gcgcgaagcc ccgcccgtcc cggcggggct ttggcgcgcg
     4801 tgcaggagcg cccgggcacg gcggcgaatc tgcagggcag cggccgtcgt cacccaggag
     4861 cggcccgaac caccgtggag gtcgtgtgac cgtgcgccgg cgactgttcg ccctgtccct
     4921 gctcgtcgtc ccggcgctgg cgctggcgcc cgccggcgcg ggcccgctgc cggacaaccc
     4981 ctggctggag cggcgcgtgc tcaacatcgc ccaccagggc ggggagatcg aggcgccgtc
     5041 ggacaccctc tacgcgttca agaccgccgt ggccaagggc gtggacatgc tggagctgga
     5101 tgtccacgcc accagtgacg gccacatcgt ggtgctgcac gacaccacgg tgaaccggac
     5161 caccgacggc gccgggcggg tcgacgcgct gacgctcaag gagatccagc gcctggacgc
     5221 cgcccactgg ttcgtcgagg gctgcggcac ctgccacggg cggcccgccg ccgactacac
     5281 gctgcgcggc atcgcgaccc accagcgcaa ggcgcccagg ggcttcacct ggaaggactt
     5341 ccgcatcccc acgctggagg aggtcctgaa gacgttcccg gacgtgccca tcaacatcga
     5401 gatcaagaac accgcgcccg acacgcccgc gtacgagcgc acgctggccg acctgctcgc
     5461 cgagtacggc cgcgacgacg acgtcgtggt cgtgagcttc ctggaccacg cgatcgagca
     5521 gttcaagctg tgggcgccgc acgtgcacac cgccaccgcg acgggcgaga cggccgcgtt
     5581 ctgggccggc agccagcagg cactgcccgg cgcgccgaac ccccgctacg tcgccttcca
     5641 ggtgcccgtc acgttcaacg gggtcaccgt ggtcaccgaa gacttcgtgc gggacgcgca
     5701 cgccaacggc atggccgtgc acgtctggac gatcaacgac cgcgccgaga tggagtggct
     5761 gctcgacatc ggcgtggacg ggatcatgac cgacgtgccg accgtgctgg aggagctgct
     5821 ggccgagcgc ggccagaaca gctagcccgg ccgccgctcg tggtggcgca gccccggggt
     5881 gcgccgcgcc tccttgagca gcgcctcgtg gacgtggcgc cggccgtcca tgagctcctc
     5941 gcgcacgcgg cgctccgcgt cgcgcaccga gtcgtagtag gtcgcgtcga actcctccac
     6001 gagctggaac gtccagcggc cctccaccac gttgcggccg atgacgtcgc gccgcagcag
     6061 cgcggccagc ccgccgtggc cggcgtcgtc gagcaggtcc gccgcgtcgc ccagcaggaa
     6121 gtcggcgcgg ccgatcatct ggtgcaggtc gtacagccgc ccccgagccc gctcgaccca
     6181 ctccagggcc tcgctgacct tcccggcggc ctccacctcg cgcggcgtcg cgtgcggcgg
     6241 cggggcgtgc gggtcgtcgg gcggcagggt caccggccgc ccccggggcg ggccgcgcgg
     6301 ctggccgcgt aggcgggatg gcgcaggcgg ttcagcagcc ccgacgggcg caggtcgacg
     6361 ccggcgttcc acgggtcgaa ggccagttcg gcgtgctcgg cggcggacag gcggtgctcc
     6421 ggcagcagca cggcccacgt gcgccacggc ccggtcgtgg tggcggccag caggcgcacc
     6481 gccgccggcg cggcgcccgc ggccatccgg tccacggacg ggccgtcctc cgccgcggcc
     6541 gcgagcatca ccgtgccggc aggcgtggcg tagggcagca cgctcgagca gggccgcgac
     6601 gccaggcgcc gcgccggcac cagcaggtga cgcagcacgg gcggcgcggc ggaactcgcg
     6661 aacagcaggt cgcggtggcg tcccggcccg tcggcgtcga gtgcgcgcac cgcgatgccc
     6721 agcacgtcgg gcaggggatc gggcaggccg gcgcccttgg agaagcgcac cagcgcgagt
     6781 tgctccccgg gctcgtccag gaacgccgat ccggtgcccg ggtccaggcc ggtgcgccgc
     6841 agggtgccgc ggaaggcgac accacgggga tggaacaccc gggcgtgccg cagccacgac
     6901 aggccgccga aggcgccggc cagcgaagcg gccaagggcg ccgggacctc gggacggaat
     6961 cgcgtcttca cgcgctccgg cgtaccccgc ccgggggctt ggcacgcgat gcgtacccgt
     7021 tgcctacgcg ctcccgccgc cgccctacgc atgtcggctc gcaagcgctc gtcccttcag
     7081 gtgaaacagg cccggcgcgc ttcaccgcct cgattcgggc accgaacggc atgggaggca
     7141 acgagtcgac gaggagacga acgcatggaa gcggtcaacg ggcgcgggca gttgctcgac
     7201 gcgggaatcg tggcgctggc cggcgccacg ctcgggcagt tcgccggcga gctgcctctg
     7261 ctgctgggct tggccggcct ctcggtgctg cggttggcca tggggctgcg gcctcgcctc
     7321 ggcagggcgc ggctgcgccg ccgcgtgcca cggctgctgc gcgtggtgcg gttggcgcga
     7381 ccacgtcggc gccgcgatgc ggataccggg cagctccagc tggtgctgcc ggaggcgccc
     7441 gccgtccacg acgacgcgcc ggagctggcg gtcatcgacg cgctgaccgg cctgccgaac
     7501 cggcgcggcg tcgagcgcag cctcgacgac gcctgccgcc aggcggtggg cgccaaccag
     7561 ccgctcggcg tgctggtctt ccaggtgcgc gacgcggggc aggtccggcg gctgttcggg
     7621 ccgcaggccg ccagggacgt cctgcgccag acgggcacgc gcctgtccaa ggcgctcggt
     7681 gacgccggcc tcgtcggccg ctgggccggc gaggagttcg tcgcggtcgt gcccgggctg
     7741 cgcgcggagg cggcggccct gctggcggag cgcttccgcc gcgtgctcga ggccgacccc
     7801 taccggctga gctccggccg gcgcgtcccc gtggcgatgg ccgccggctg ggcggccagt
     7861 ccccgcgacg gcatcagcgg ccagttcctg gtctcgcgcg cccaggggcg cgcggcggcg
     7921 gcgcgcagcg acgcgggagc gctggcgctc agctgacggg cagccccttc cggacgggca
     7981 gcgcggcgtt tacgctgctg gcgggtcccg ccccggggcg ccccccgggg gtcgtatggg
     8041 gggccgatcc gggggagccg gatgtcggga cccgtccccc agacggaaag ggcagcacat
     8101 gagcatcgac gaggtcctgg tggaacgggc gtcgatcgac gaggagatcc acggcaagac
     8161 gctgctggac ctgttcgagc gcaacgcccg tgagcacggc gaccgtccgg ccctgaactg
     8221 gatggacgcg caggggcagt ggcagtcggc gacgtggcgg gagtaccgcg agcgggcggt
     8281 gcgcgccgcc atgggcctgc gctcgctggg cgtcgacccc ggcgacttcg tggcgatcat
     8341 ggcgggcaac cgtcccgagc acgtgatcgc ggatgtggcc gccatgtaca acggcgccac
     8401 cgcggtgtcc ctgtacaaca cgctggcccc cgagcagatc gtctacgtgg cggacaactg
     8461 cggcgccaag atcgccatcc tggagaaccg cgactacttc aagcgctggg agcagatcca
     8521 cgaccagctc ccgcgctgcg agaaggtcgt gctcatggag cacgccgacg agttccgcgg
     8581 ctacgactgg gtgctgtcct gggacgaggt catggccgcg ggcgagcagg cactggcggc
     8641 gcagccggac gcggtggagg agcggcgcaa gacgctgacg ccggagacgc cggccacggt
     8701 gatctacacg tccgggacca ccgggccgcc gaagggcgtg gtgctgaccc accgcaacat
     8761 cctgtgggag ctcaccgcca ccgaccactt caacgcgttc ccggacttcc cgcgcggcat
     8821 cagctacctg ccgctggcgc acgtggcgga gcgcagcttc agccactgga acggcatcaa
     8881 caaggccggc tccacctact tctgcccgga gatcacgcgg gcgatggagg tcgtgccggt
     8941 ggcgcggccg caggcgttcg tcgcggtgcc ccgtgtgtgg gagaagctgc acgccggcct
     9001 gctggcggcg ctgcaggccg agcccgacga gcgcaagcgc aagatcgcgc tgaaggccgt
     9061 cgcggtgggc caggaggtga tcgagcgcac ccagcgcggc gactccgtgc cgttgaagct
     9121 gcgcctgcag caccggctgt tcgacaagct ggtctactcc aagatccggg agaagatcgg
     9181 gctggacgcc tgcgagctgg cgctgtccgg cgcggcgccc atcgcccggg acatcctggt
     9241 gttcttcgcc ggcatcggcc tgccgatcca cgaggtctac ggcatgagcg agaccaccgc
     9301 ggtcacgcac ggcaacccgc tcggggcgat caagatcggc accgtcggcg tgccccttcc
     9361 cggtgtggag tgccgcatcg ccgaggacgg cgaggtgctg gtgcgcggcg gcaacatgac
     9421 cccgggctac cacaaccggc cggaggccac ggcggagctg ttggacgacg agggctgggt
     9481 gcacaccggc gacctgggca ccgtggacgc cgacggctac ctgaccatcg tcgggcgcaa
     9541 gaaggagctg atcatcacgg cgggcggcaa gaacatctcg ccgaacaaca tcgagggcgt
     9601 cctcaaggag cacccgctga tcggccaggt gtgctgcgtc ggggacatga agccgttcat
     9661 ctccgccctg atcgtgctgg acggcgaggc ggcgccggtg tgggcggcgc agcacggcgt
     9721 gcccttcacc gacttcgcga gcttctcccg cgacgagcgg gtgctcagcg aggtcgccag
     9781 cgcggtcgag gcggccaacc agcggctggc caaggtggag cagatcaaga agtggcgcgt
     9841 gctcgagcgc gagtggacgc ccgagaccga ggagctcacg cccacgctga agctcaagcg
     9901 caacgtgatc cacgacaagt acgccgcgga catcgaaagc ctgtactcgt cgtgaccggc
     9961 aggtcccggt gagccgggag ggggcgtccc gaggggcgcc ccctcccatc cggaaatgat
    10021 catttcgggc catggccggg ctcgccgcgc ctccacatag tgggggtgtt cccccgccga
    10081 cggtggtcgg ggtggcggcg gagaggcgcg gccgtgcagg acatggcaca gggcatcgag
    10141 tacgtcaacg acgtgctgtt cgtcggcttg gccgtcgtgg cgttccgcca gtggcgcagc
    10201 cgccgcgacc cgcagaccgc ctgggtggcg gtcacgttcg tcctgctgtc ggcggtcgtg
    10261 atcggcggcc tggtcctgcc ggaatccggt gagcagccct ggcgcgtctg ggccaccaag
    10321 gcgctgctgg tcggcctgct gttgttcccc tgggcgttgt accgcttcac cgtgtcgttc
    10381 cagggctccc gcccgcgtgt ggagacggtc gcccgtgcgc tgacggccgg tgtgctcgcc
    10441 ctcaccgtcc tgctgccgga cgtccccgcg ccggccgtca gcgcacgggc gaccgtctcc
    10501 ac
//
LOCUS       DATMKC010000141         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_270615, whole genome shotgun sequence.
ACCESSION   DATMKC010000141 DATMKC010000000
VERSION     DATMKC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270615"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VNU01_04220"
     CDS             <1..1354
                     /locus_tag="VNU01_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31855.1"
                     /translation="PAAASCVPPLMRGYTTVDDPMPHGDGHDHADPAQHRLARNATLL
                     AVDDLRRFGWTETTVVGAHALARHGDLLAVAVNGGRDGSQQGFHLFDVSVADAPRHLG
                     MFEAGVSVAGDRTIAFSHDGRTVFLGYEGAARPGVAAVDVSDPSRPRETHFWRDPQGF
                     GSHTVSAGTIDGVQHVFSLAAGINILRYDPSGFQLVGKYVTADGLGFLDAPGSSTGIQ
                     TYVLRSLYGHDMDFYLDAVTGKPLLLVAYAYDGAKILDLSVPSAPVLQARWRPPPAAD
                     HAHYTHSAVAERSSDGRLLWVVGSETFEQENQGIASPIWILDATDAVAAPPLAAAPEH
                     LATWRNPGGSPAGHLGSSVHFFRLQQGLLYLSHYHGGIWAFDLRDSGAWRDVRAFGHI
                     LPVGPDPVAPPEGCCINYDLGRVPMVFDVEAGAEPGVAYGADLVQGLSVVRFDVPAGA
                     "
     gene            1396..1959
                     /locus_tag="VNU01_04225"
     CDS             1396..1959
                     /locus_tag="VNU01_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31856.1"
                     /translation="MRCALVLGLLLLAGCADPGGGEPRRTCQWSEGPPGEVVRGGFFA
                     HDPNDNHRADELGRDALENGGRPLAAMTLSFHPWQTPTGENQTQGVMVRDGRVTLRFE
                     RMDDGTPLQAYRDQRRGEPRDTWTFEQQFEKDFTLTVPFDAAGGGPSGVRAVWTFERN
                     LDGNADTQSVAFIHYNVQRWYEECFDF"
     gene            complement(2087..2359)
                     /locus_tag="VNU01_04230"
     CDS             complement(2087..2359)
                     /locus_tag="VNU01_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWH31857.1"
                     /translation="MKAVRLLIDGKVQGVSFRESMREEAERLGVFGWVRNLEDGRVEA
                     WVEGEARAVARLVDWARRGPPRARVESLDQEDGQPEGHDAFLVRRA"
     gene            complement(2356..>2692)
                     /locus_tag="VNU01_04235"
     CDS             complement(2356..>2692)
                     /locus_tag="VNU01_04235"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31858.1"
                     /translation="PVADGRPADAAGGPAAAGRGAAGTSAEAEGSVTIQQDAAMLATI
                     LGGGGKRPYTFAAGRKGLLFVASGAAVAGGERLERGDSLFVAGSGTLPVEAEGAVHLV
                     LFDVPAVAP"
BASE COUNT          360 a         1081 c          912 g          339 t
ORIGIN      
        1 ccccgccgcg gcaagctgcg tccctcccct catgcgcggc tacaccaccg tggacgaccc
       61 gatgccgcac ggcgacggcc acgaccacgc cgaccccgcg cagcaccgcc tcgcccgcaa
      121 cgcgaccctg cttgccgtcg acgacctgcg gcgcttcggc tggaccgaga cgaccgtcgt
      181 cggcgcccac gcgctcgcgc gccacggcga cctgcttgcc gtcgccgtca acggcggcag
      241 ggacggctcg cagcagggct tccacctgtt cgacgtgtcg gtggcggacg cgccccggca
      301 cctcggcatg ttcgaggcgg gcgtgtccgt cgccggcgac cgcacgatcg ccttcagcca
      361 cgacggccgc accgtgttcc tcggctacga gggcgcggcg cgcccgggtg tggccgccgt
      421 cgacgtctcc gacccgtccc ggccgcggga gacgcacttc tggcgcgacc cccaagggtt
      481 cggcagccac acggtctccg cgggcaccat cgacggcgtg cagcacgtct tctcgctggc
      541 cgccggcatc aacatcctgc gctacgaccc ctccgggttc cagctcgtgg ggaagtacgt
      601 caccgcggac gggctcggct tcctggacgc ccccggctcc agcacgggca tccagacgta
      661 cgtcctgcgc agcctctacg ggcatgacat ggacttctac ctggacgccg tcaccgggaa
      721 gcccctgctg ctggtggcgt acgcgtacga cggcgcgaag atcctcgacc tcagcgtccc
      781 gtccgcgccg gtgctgcagg cccgctggcg cccgccgccg gccgccgacc acgcgcacta
      841 cacgcacagc gccgtggcgg agcgcagcag cgacggccgc ctcctctggg tcgtcggcag
      901 cgagaccttc gagcaggaga accagggcat cgcaagcccg atctggatcc tggacgcgac
      961 cgacgcggtc gccgccccgc cgctggccgc cgcaccggag caccttgcca cctggcgcaa
     1021 ccccggcgga agccccgccg gacacctggg cagcagcgtg cacttcttcc ggctccagca
     1081 gggcctgctg tacctcagcc actaccacgg cggcatctgg gccttcgacc tgcgcgacag
     1141 cggcgcctgg cgcgacgtgc gggcgttcgg ccacatcctc cccgtcggtc ccgacccggt
     1201 cgccccgccg gagggatgct gcatcaacta cgacctcggc cgcgtcccca tggtcttcga
     1261 cgtggaggcc ggcgcggagc cgggcgtggc gtacggcgcc gacctcgtgc agggcctgag
     1321 cgtcgtgcgc ttcgacgtcc ccgccggggc gtgaggcatc gcaacggcca agtccgtccg
     1381 tccggtccgg cggacgtgcg ctgcgcgctg gtgcttggac tgctgctgct tgccggctgc
     1441 gcggacccgg gcggaggcga gccgcggcgc acgtgccagt ggtcggaagg gccgccaggc
     1501 gaggtcgtgc gcggcggctt cttcgcccat gacccgaacg acaaccaccg cgccgacgag
     1561 ctggggcggg acgcgctgga gaacggcggg cgcccgcttg ccgcgatgac gctgtcgttc
     1621 cacccctggc agacgccgac gggcgagaac cagacgcagg gcgtcatggt gcgcgacggg
     1681 cgcgtcacgc tccgcttcga gcggatggac gacgggacgc ccttgcaggc ctaccgggac
     1741 cagcggcgcg gcgagccccg cgacacctgg accttcgagc agcagttcga gaaggacttc
     1801 accctcaccg tccccttcga cgccgccggc ggcggcccgt ccggcgtgcg cgccgtgtgg
     1861 acgttcgagc gcaacctgga cggcaacgcg gacacgcaga gcgtggcctt catccactac
     1921 aacgtccagc gctggtacga ggagtgcttc gacttctgag gcccaccgca cactgcccta
     1981 gcccggcctt gcgcggccgg ggcgggtccg cccggctgcc ccggacggct gcggcagcgc
     2041 agccacgaga tgcctgacga ccggcgccgg tccgtggcgc caccgcctat gcgcggcgca
     2101 ccaggaacgc gtcgtgcccc tccggctgcc cgtcctcctg gtccagagac tcgacgcgcg
     2161 cccgcggcgg tccgcgccgc gcccagtcga cgaggcgggc caccgcgcgc gcctcccctt
     2221 ccacccaggc ctccacgcgg ccgtcctcga ggttgcgcac ccagccgaac acgccgaggc
     2281 gctccgcctc ctcccgcatc gactcgcgga acgagacgcc ctggaccttg ccgtcgatga
     2341 gcagccgcac ggccttcatg gcgccaccgc cggcacgtcg aacagaacga gatggacagc
     2401 gccttccgcc tccaccggca aggtgccgga tccggcgacg aagaggctgt ccccgcgctc
     2461 caggcgctcc ccgcccgcca cggcggcgcc ggaagccacg aagaggagtc ccttgcggcc
     2521 agcggcgaac gtgtaaggcc gcttcccgcc accgcccagg atcgtggcca gcatcgctgc
     2581 atcctgctgg atcgtgacgg agccctccgc ctccgccgac gtgcctgccg ccccccggcc
     2641 ggccgccgcc gggccgcccg ccgcgtcggc gggcctgccg tctgcgaccg gg
//
LOCUS       DATMKC010000142         8822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_211397, whole genome shotgun sequence.
ACCESSION   DATMKC010000142 DATMKC010000000
VERSION     DATMKC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211397"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..616
                     /locus_tag="VNU01_04240"
     CDS             92..616
                     /locus_tag="VNU01_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31859.1"
                     /translation="MLEVIQSDHNLVGEALALALLLGVAALSLAGMRRGYRRRRAAAR
                     DRYGELAPVPAPAREPDGQALYTGTTRAGSRVERVALPELWGRWRCDWWADAGRVVLR
                     GPGGRVVALDGIVECGLVGAHAGRAVGRGRIAVVRWRLGGHEVDTGLQFASAEEALGF
                     TARIEAAREGQRHA"
     gene            609..1727
                     /gene="carA"
                     /locus_tag="VNU01_04245"
     CDS             609..1727
                     /gene="carA"
                     /locus_tag="VNU01_04245"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWH31860.1"
                     /translation="MHEALLVLEDGTSFRGQGFGADGTAFGEVVFNTGMSGYQEVLTD
                     PSYHRQLVTMTYPHQGNYGITDADAESSRVQVGGFIVREQARRHSNWRAERSLDDELR
                     AYGAVGISEIDTRRLTRHIRSAGAMRAAISTEVLDVDALVERVRASQGTEGWNGAEEV
                     SAPEAYDFPAEAGRGEARFRVVAYDFGIKRNILRLLAAHGCDVTVVPALTPADEVLAR
                     EPDGVFLSNGPGDPAAVRNGIAAIKDVLAAEVPLFGICLGHQLLGLAAGAKSYKMVFG
                     HRGINQPVHNTEREAVEITSHNHGFAIDAETLPSDGPFGRVSQTHVNLNDHVNEGLRL
                     HDVPAFSVQYHPEAAPGPHDARYLFQQFCDLMTAKGNA"
     gene            1727..4996
                     /gene="carB"
                     /locus_tag="VNU01_04250"
     CDS             1727..4996
                     /gene="carB"
                     /locus_tag="VNU01_04250"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWH31861.1"
                     /translation="MPKRTDINSILILGSGPIVIGQACEFDYSGAQACKVLRREGYRV
                     ILVNSNPATIMTDPEFADATYVEPLTPEMVAKVIAKEKPDALLPTLGGQTGLNVAMAL
                     HQRGTLDEHGVQLIGASTHAIDLAEDRKHFAAAMREIGLDTAASTEANSVEQALAFAD
                     THGFPLLVRSSFTLGGAGSGVAHSLEQFKRMVAEGIAASPIGEVQIDESLLGWKEYEL
                     EVMRDRADNCVVICSIENLDAMGVHTGDSVTVAPAQTLSDVEYQDMRDAAFAIMRKVG
                     VETGGSNVQFAVDPATGRQVVIEMNPRVSRSSALASKATGFPIAKIAALLAVGYTLDE
                     IPNDITRETMAAFEPTIDYVVTKIPRFTFEKFPGADPELTTRMKSVGEVMAIGRTFKE
                     SLGKALRSMENGTLGLTGAEDPRDDEALAQALRVPHDGRVLAADAALTRGWGVERVAE
                     LTGYDPWFVDQMLQVVELREKVRALGSPAALDAATLRQAKRCGLGDGALARVLGSTEG
                     DVRARRLELGVVPVFKTVDTCAAEFAAQTPYHYSTYEEETEVAPSDRERVIILGSGPN
                     RIGQGIEFDYCCVHAVYALRDAGYETVMVNCNPETVSTDYDTADRLYFEPLTLEDVLE
                     VVDAERAASGREPGLLVQLGGQTPLKLARDLEAAGCRILGTLPEAIDRAEDRGRFGAI
                     LDELGIPQPPGGMAGSFDEAMAVAERIGFPVLVRPNYVLGGRAMEIVYTPEQMRVWLE
                     ANAAEGTVLVDKFLEGAVEVDVDAVYDGQELYAGGIMEHIEEAGIHSGDSACVIPPYT
                     LGRSQQLRLRDYTEAIAAALGTRGPINIQYAIKDDVIFVIEANPRASRTMPFVSKVTG
                     VPLAKIAARVMAGATLAELRAEGWVPDRDTVLGPPLAHIAVKEAVLPFNRFPGVDTRL
                     GPEMRSTGEVMGIDADFGVAFAKSQAGTGTMVLPAKGTVFASIANRDKRAMLFPIKRL
                     AEMGFTIVATEGTAEALQHAGVAAETVRKFSEGGPNIVDRITAGEIDLVLNTPMGSGP
                     RSDGYEIRTASVVAGIPCITTMSGIMAAIQGIEALRSDTISVRSLQDWHAAQDAGGVK
                     S"
     gene            4993..5916
                     /locus_tag="VNU01_04255"
     CDS             4993..5916
                     /locus_tag="VNU01_04255"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWH31862.1"
                     /translation="MSSIVSRGSTPSVQGRSSGPLRGMCEVLATRRVGAYQSITFVAP
                     EMAERAAPGQFISIAVDAAGTLLRRPFSIFSVSQHGPWAGTVEVVFDVIGPGTKWLAE
                     RSKHDVVDVVGPLGKPFPLPAQPVSCLLVGGGYGAAPLLYLAGVLQQGGFRTDIILGA
                     KSQDRIFNAIEAKRLSATAQFTTEDGTLGTQGRVTDVMDRVIEQARTGVIYACGPMPM
                     LAAVAHVARRHRIPSQVAVEEAMACGVGVCWTCVLPYAKKDGVQNLRACVDGPVFNGA
                     RIDWERLGLGEVVEAPPLPDPETDPNVELRP"
     gene            5913..6875
                     /locus_tag="VNU01_04260"
     CDS             5913..6875
                     /locus_tag="VNU01_04260"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWH31863.1"
                     /translation="MTAVEPQRSGRALRPDQVDLRIDLGPLVLANPFVTASGCFASGQ
                     EINRFYDVNRLGAVVVKSITLEPRPGLPTPRMAETPSGMLNAIGLQNPGIDAWMANDL
                     PWLQRAGVPTIVSIAGKTAQEFRILAERLRGVPGVIAIEANISCPNVEDRNIVFACRE
                     TSTKEVISQVSRVATVPVFAKLTPDVTDIVSIAATARASGAAGVSVINTLLGMAIDVE
                     TRRPKLAAITGGLSGPAIKPVALRAVHQIHQALPDLPIIGMGGIRTVEDVVEFLIAGV
                     SAVAIGTANFTNPLVAAELLDELPRWCAARGIRSVTELRGDLRP"
     gene            6872..7465
                     /gene="pyrE"
                     /locus_tag="VNU01_04265"
     CDS             6872..7465
                     /gene="pyrE"
                     /locus_tag="VNU01_04265"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWH31864.1"
                     /translation="MIDVDKTFRELEVLRTGHFQLSSGKHSDTYLQCQLALQRPDVAL
                     ELGRALAERLRAQGAADVDVVASPALGGILAGFAVASALGVRFVFSERKGGPDGRAMV
                     FRRGQRVEPGERVLVVEDVVTTGGSAMEVVALCEQAGAKVAGTAAIVDRSAGLPEEER
                     PPLPPVALLTVTAQTWTAQECPRCAAGDPVDSPGSRR"
     gene            7540..7860
                     /gene="mihF"
                     /locus_tag="VNU01_04270"
     CDS             7540..7860
                     /gene="mihF"
                     /locus_tag="VNU01_04270"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HWH31865.1"
                     /translation="MPLPELDDDSRRQALEKAAEARRVRAELKQRLKAGEIDFAEVLR
                     QADTNEIIGKTKVSAVLEALPKVGKVRARKLMERLDISPSRRVRGLGANQREKLLKEF
                     EADA"
     gene            7886..8488
                     /gene="gmk"
                     /locus_tag="VNU01_04275"
     CDS             7886..8488
                     /gene="gmk"
                     /locus_tag="VNU01_04275"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWH31866.1"
                     /translation="MDEVEAPGTGRLVVISGPSGVGKGTVVDALRERRPSLRVSVSAT
                     TRPRRPHEVHGEHYFFLSREQFDGLVAADGFLEWAEFNGNRYGTPLQAVLDALAEGHT
                     VLLEIEVQGALQVRDRVPDALLVFLMPPDAGELARRLHGRGTESSDEIADRLSIADQE
                     MALAQALRGDVFDHVVVNRVVGEAAEEILRILDGPSATPA"
     gene            8508..>8822
                     /gene="rpoZ"
                     /locus_tag="VNU01_04280"
     CDS             8508..>8822
                     /gene="rpoZ"
                     /locus_tag="VNU01_04280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWH31867.1"
                     /translation="MSPIARIDDLLNKVDSKYTLVHLAARRAREINSYYHSLGEGLGQ
                     FTPPLVDQVDSNKPLSIALEEIAEAKIVVAEGRDALAMAEELLGGGADTGAQVVSLSD
                     EDG"
BASE COUNT         1287 a         3144 c         3236 g         1155 t
ORIGIN      
        1 gccctaccac ggcatgagcc tgcgcggccg ccccgtccac acgctgctgc gcgggcggtt
       61 caccttccgc gacggggagg tgacgggcta ggtgctggag gtcatccaaa gcgaccacaa
      121 cctcgtcggc gaggcgctcg cgctcgcgct gctgctcggt gtcgccgccc tgtcgctggc
      181 cgggatgcgc cggggctacc ggcgccgccg cgccgcggcg cgcgaccgct acggcgagct
      241 cgcgccggtg cccgcgccgg cgcgggagcc cgacggccag gcgctgtaca ccggcacgac
      301 gcgagccggc tcccgcgtgg agcgcgtggc gctgccggaa ctgtgggggc gctggcgctg
      361 cgactggtgg gccgacgccg gcagggtcgt cctgcggggg cccggcgggc gcgtcgtggc
      421 gctcgacggc atcgtggagt gcgggctggt cggggcgcac gccggccggg ccgtgggccg
      481 cggccgcatc gccgtggtgc gctggcggct cggcgggcat gaggtcgaca ccggcctgca
      541 gttcgcgagc gccgaggagg cgctgggctt caccgcccgg atcgaagcgg caagggaagg
      601 acaacggcat gcatgaggcc ctgctcgtcc tggaggacgg caccagcttc cggggccagg
      661 ggttcggggc ggacggcacc gcgttcggcg aggtcgtctt caacaccggc atgagcggct
      721 accaggaagt gctcaccgac cccagctacc accgccagct cgtcaccatg acctaccccc
      781 accagggcaa ctacggcatc accgacgccg acgccgagtc ctcgcgggtc caggtgggcg
      841 gcttcatcgt gcgcgagcag gcgcgccgcc actccaactg gcgcgccgag cgcagcctgg
      901 acgacgagct gcgcgcgtac ggcgccgtgg gcatcagcga gatcgacacc aggcgcctca
      961 cccggcacat ccgctcggcg ggcgccatgc gcgcggcgat ctccaccgag gtgctcgacg
     1021 tcgatgccct ggtggagcgg gtgcgcgcca gccagggcac ggagggctgg aacggggcgg
     1081 aggaggtctc ggcccccgag gcctacgact tccccgccga ggcgggccgc ggcgaggcgc
     1141 gcttccgcgt ggtcgcctac gacttcggca tcaagcgcaa catcctgcgg ctgctcgccg
     1201 cgcacggctg cgacgtgacg gtggtgccgg ccctgacgcc cgccgacgag gtgctcgccc
     1261 gggagccgga cggggtgttc ctgtccaacg gccccgggga ccccgccgcg gtgcgcaacg
     1321 gcatcgcggc gatcaaggac gtcctcgcgg ccgaggtgcc gctgttcggc atctgcctgg
     1381 ggcatcagct gctcggcctg gccgccgggg caaagtccta caagatggtg ttcggccacc
     1441 ggggcatcaa ccagcccgtg cacaacacgg agcgcgaagc cgtcgagatc accagccaca
     1501 accacggttt cgcgatcgac gccgagacgc tgccctccga cgggccgttc ggccgcgtga
     1561 gccagacgca cgtgaacctg aacgaccacg tcaacgaggg cctgcgcctg cacgacgtgc
     1621 ccgccttcag cgtgcagtac caccccgagg ccgcgcccgg gccgcacgac gcgcgctacc
     1681 tgttccagca gttctgcgac ctgatgaccg cgaagggcaa cgcctgatgc cgaagcgcac
     1741 cgacatcaac tcgatcctga tcctgggctc cgggccgatc gtgatcggcc aggcctgcga
     1801 gttcgactac tccggggcgc aggcgtgcaa ggtgctgcgc cgcgagggct accgggtgat
     1861 cctggtgaac tcgaacccgg cgacgatcat gaccgacccc gagttcgccg acgccaccta
     1921 cgtcgagccg ctcacgccgg agatggtcgc gaaggtcatc gccaaggaga agccggacgc
     1981 gctgctgccg acgctcggcg ggcagaccgg cctgaacgtc gcgatggcgc tgcaccagcg
     2041 cggcacgctg gacgagcacg gcgtgcagct catcggcgcc agcacgcacg ccatcgacct
     2101 ggctgaggac cgcaagcact tcgccgccgc catgcgcgag atcggcctgg acacggcggc
     2161 ctcgaccgag gccaacagcg tcgagcaggc gctggcgttc gccgacacgc acggcttccc
     2221 cctgctggtg cgctccagct tcacgctcgg cggcgccggc tccggcgtgg cgcacagcct
     2281 ggagcagttc aagcgcatgg tggccgaggg catcgcggcg agccccatcg gcgaggtgca
     2341 gatcgacgag agcctgctcg gctggaagga gtacgagctc gaggtcatgc gcgaccgcgc
     2401 ggacaactgc gtggtcatct gctcgatcga gaacctcgac gccatgggcg tgcacaccgg
     2461 cgactccgtc accgtcgccc ccgcgcagac gctgtccgac gtcgagtacc aggacatgcg
     2521 cgacgccgcg ttcgcgatca tgcgcaaggt gggcgtggag accggcggca gcaacgtgca
     2581 gttcgcggtc gaccccgcca ccggccgcca ggtcgtcatc gagatgaacc cgcgcgtgtc
     2641 acgctcctcg gccctggcgt ccaaggccac cggcttcccg atcgccaaga tcgccgcgct
     2701 gctggcggtg ggctacacgc tggacgagat ccccaacgac atcacgcgcg agacgatggc
     2761 ggcgttcgag ccgaccatcg actacgtggt caccaagatc ccgcgcttca cgttcgagaa
     2821 gtttcccggc gccgacccgg agctgaccac gcgcatgaag agcgtgggcg aggtcatggc
     2881 gatcggccgg acgttcaagg agtcgctggg caaggcgctg cgctccatgg agaacggcac
     2941 gctcggcctc acgggcgccg aggacccgcg cgacgacgag gcgctcgccc aggcgctgcg
     3001 cgtgccccac gacggccgcg tgctggccgc cgacgccgcg ctgacgcgcg gctggggcgt
     3061 cgagcgcgtc gcggagctca ccggctacga cccctggttc gtcgaccaga tgctgcaggt
     3121 ggtggagctg cgcgaaaagg tccgcgcgct cggctcgccg gccgccctgg acgcggcgac
     3181 gctgcgccag gccaagcgct gcgggctggg cgacggcgcg ctcgcgcgcg tgctcggctc
     3241 cacggagggc gacgtgcgcg cgcggcgcct ggagctcggc gtggtgcccg tgttcaagac
     3301 cgtggacacc tgcgccgccg agttcgccgc ccagacgccg taccactact ccacctacga
     3361 ggaggagacc gaggtcgcgc cgagcgaccg cgagcgcgtg atcatcctgg gctcggggcc
     3421 caaccgcatc ggccagggca tcgagttcga ctactgctgc gtccacgcgg tctacgccct
     3481 gcgcgacgcc ggctacgaga ccgtgatggt gaactgcaac ccggagacgg tctccaccga
     3541 ctacgacacc gccgaccggc tgtacttcga gccgctgacg ctggaggacg tcctggaggt
     3601 cgtcgacgcc gagcgcgccg ccagcggccg cgagccgggc ctgctcgtgc agctcggcgg
     3661 ccagacgccg ctgaagctgg cgcgcgacct ggaggcggcc ggctgccgga tcctcggcac
     3721 gctgccggag gcgatcgacc gggcggagga ccgcggccgc ttcggcgcga tcctggacga
     3781 gctcggcatc ccgcagcccc ccggcggcat ggccggctcc ttcgacgagg cgatggcggt
     3841 cgcggagcgc atcggcttcc cggtgctggt gcggccgaac tacgtgctgg gcggccgcgc
     3901 gatggagatc gtgtacacgc ccgagcagat gcgcgtgtgg ctggaggcca acgccgccga
     3961 gggcaccgtc ctggtcgaca agttcctgga gggcgccgtc gaggtcgacg tcgacgccgt
     4021 ctacgacggc caggagctgt acgccggcgg gatcatggag cacatcgagg aagccggcat
     4081 ccactccggc gactccgcct gcgtgatccc gccgtacacc ctcggccggt cgcagcagct
     4141 gcggctgcgc gactacaccg aggcgatcgc cgccgcgctc ggaacccgcg gacccatcaa
     4201 catccagtac gcgatcaagg acgacgtgat cttcgtcatc gaggcgaacc cgcgcgccag
     4261 ccggacgatg ccgttcgtgt ccaaggtgac gggcgtgccg ctggccaaga tcgccgcgcg
     4321 cgtcatggcg ggggccacgc tggccgagct gcgcgccgag ggctgggtgc ccgaccgcga
     4381 caccgtgctc ggcccgccgc tggcgcacat cgcggtgaag gaggcggtgc tgccgttcaa
     4441 ccgcttcccg ggcgtggaca cccggctggg cccggagatg cgctccaccg gcgaggtcat
     4501 gggcatcgac gcggacttcg gggtcgcgtt cgccaagagc caggccggca ccggcacgat
     4561 ggtgctgccg gccaagggca ccgtgttcgc cagcatcgcc aaccgcgaca agcgcgcgat
     4621 gctgttcccc atcaagcgcc tggccgagat gggcttcacg atcgtcgcga cggagggcac
     4681 cgccgaggcg ctgcagcacg ccggcgtggc ggcggagacg gtgcgcaagt tcagcgaggg
     4741 cggccccaac atcgtcgacc gcatcaccgc cggcgagatc gacctggtgc tgaacacgcc
     4801 catgggctcg gggccgcgct ccgacggtta cgagatccgc acggcgtccg tggtcgccgg
     4861 catcccgtgc atcaccacga tgtcggggat catggcggcc atccagggca tcgaggcgct
     4921 gcgctccgac accatctcgg tgcgctccct gcaagactgg catgccgccc aggacgccgg
     4981 aggggtgaag tcgtgagcag catcgtcagc cgaggcagca cgccgtccgt gcagggccgc
     5041 tccagcgggc cgctgcgcgg catgtgcgag gtgctcgcga cacgccgggt gggcgcctac
     5101 cagtccatca cgttcgtggc cccggagatg gcggagcgtg ccgcgccggg gcagttcatc
     5161 tccatcgccg tggacgcggc ggggacgctg ctgcggcggc cgttctcgat cttctccgtc
     5221 agccagcacg gcccgtgggc cggcacggtc gaagtcgtct tcgacgtcat cggccccggc
     5281 accaagtggc tggccgagcg ctccaagcac gacgtcgtgg acgtggtcgg gccgctgggc
     5341 aagccgttcc cgcttccggc gcagccggtg agctgcctgc tggtgggcgg cggctacggc
     5401 gccgcgccgt tgctgtacct ggccggcgtg ctgcagcagg gcggcttccg cacggacatc
     5461 atcctgggcg ccaagagcca ggaccgcatc ttcaacgcga tcgaggcgaa gcggctgtcg
     5521 gccacggcgc agttcaccac cgaggacggc acgctcggca cgcagggccg ggtgaccgac
     5581 gtgatggacc gcgtgatcga gcaggcgcgc accggcgtga tctacgcctg cgggccgatg
     5641 cccatgctcg ccgcggtcgc gcacgtggcg cgccgccacc gcatccccag ccaggtggcg
     5701 gtggaggagg cgatggcctg cggcgtgggc gtgtgctgga cgtgcgtgct gccgtacgcc
     5761 aagaaggacg gcgtgcagaa cctgcgcgcc tgcgtcgacg ggccggtgtt caacggcgcc
     5821 cgcatcgact gggagcgcct gggcctgggc gaggtcgtcg aggcgccgcc cctgccggac
     5881 cccgagacgg acccgaacgt ggagctgcgc ccgtgacggc cgtggagccg cagcgcagcg
     5941 gccgggcgct gcgccccgac caggtggacc tgcgcatcga cctgggaccg ctggtgctgg
     6001 cgaacccgtt cgtcacggcc agcggctgct tcgccagcgg ccaggagatc aaccgcttct
     6061 acgatgtcaa ccgcctgggg gcggtggtgg tgaaatcgat cacgctggaa ccccggccgg
     6121 ggctgccgac gccgcgcatg gccgagacgc ccagcggcat gctcaacgcc atcggcctgc
     6181 agaacccggg catcgacgcg tggatggcca acgacctgcc gtggctgcag cgcgccggcg
     6241 tgccgaccat cgtgagcatc gccggcaaga ccgcccagga gttccgcatc ctggccgagc
     6301 ggctgcgcgg cgtgcccggc gtgatcgcca tcgaggcgaa catctcctgc cccaacgtgg
     6361 aggaccgcaa catcgtgttc gcctgccggg agacctccac caaggaggtc atcagccagg
     6421 tgtcgcgcgt cgccaccgtg ccggtgttcg ccaagctgac gccggacgtg accgacatcg
     6481 tgtccatcgc ggcgacggcg cgcgcctcgg gggcggccgg tgtcagcgtc atcaacacgc
     6541 tgctcggcat ggcgatcgac gtggagacgc gccgtcccaa gctcgccgcc atcaccggcg
     6601 gcctgtccgg gcccgcgatc aagccggtcg cgctgcgcgc cgtgcaccag atccaccagg
     6661 cgctgccgga cctgccgatc atcggcatgg gcggcatccg cacggtggag gacgtcgtgg
     6721 agttcctgat cgccggggtg agcgccgtgg cgatcggcac ggcgaacttc accaaccccc
     6781 tcgtggccgc ggagctgctc gacgagctgc cgcgctggtg cgccgcgcgg ggcatccgtt
     6841 ccgtcaccga gctgcgtgga gacctgcgac cgtgatcgac gtggacaaga cgttccgcga
     6901 gctggaggtg ctgcggaccg gccacttcca gctgtccagc ggcaagcaca gcgacaccta
     6961 cctgcagtgc cagcttgcgc tgcagcgtcc ggacgtcgcg ctggagctgg gccgcgcgct
     7021 cgcggagcgg ctgcgcgcgc agggcgccgc cgacgtggac gtggtcgcct cgcccgccct
     7081 gggcggcatc ctggccggct tcgcggtggc cagcgcgctg ggcgtgcgct tcgtgttcag
     7141 cgagcgcaag ggcggcccgg acgggcgcgc gatggtgttc cgccgcggcc agcgcgtgga
     7201 gccgggggag cgcgtgctgg tcgtggagga cgtcgtgacc accggcggct cggccatgga
     7261 ggtcgtggcg ctgtgcgagc aggccggcgc caaggtggcc ggcacggcgg cgatcgtgga
     7321 ccgttccgcc ggactgccgg aggaggagcg cccgcccctg ccgccggtgg cgctgctgac
     7381 cgtgacggca cagacgtgga ccgcgcagga gtgcccgcgc tgcgccgcgg gcgacccggt
     7441 cgactcgccc ggcagtcgcc gttaggatgc cggacgccgt tcgtcccgtg tcccgcggtc
     7501 gcaggccgcg cgctgcgggt catcttttca ggaggaccca tgcccctgcc ggagctcgat
     7561 gacgactcgc gccgtcaggc gttggagaag gcggccgagg cccggcgggt ccgtgccgag
     7621 ctgaagcagc ggctcaaggc cggcgagatc gacttcgccg aggtcctgcg ccaggcggac
     7681 accaacgaga tcatcggcaa gaccaaggtg tcggccgtgc tggaggcgct gcccaaggtg
     7741 ggcaaggtgc gcgcgcgcaa gctgatggag cgcctggaca tctcgccgtc gcggcgggtg
     7801 cgcggcctcg gcgcgaacca gcgcgagaag ctgctcaagg agttcgaggc cgacgcctga
     7861 ggcggacgac gagggccggc acaccatgga cgaggtcgag gcgcccggca cgggccgcct
     7921 ggtcgtgatc agcgggccca gcggcgtcgg caagggcacg gtcgtggacg cgctgcgcga
     7981 gcgccgccca agcctgcgcg tctccgtgtc cgccaccacg cggccgcgcc gcccccacga
     8041 ggtccacggc gagcactact tcttcctgtc cagggagcag ttcgacggcc tggtcgcggc
     8101 cgacggcttc ctggagtggg ccgagttcaa cggcaaccgc tacggcacgc ccctgcaggc
     8161 ggtgctggac gcgctggccg aggggcacac cgtgctgctg gagatcgagg tgcagggcgc
     8221 gctgcaggtc cgcgaccgtg tcccggacgc gctgctggtg ttcctcatgc cgcccgacgc
     8281 gggggagctg gcgcggcgcc tgcacggccg cggcacggag tcctccgacg agatcgccga
     8341 ccgtctcagc atcgccgacc aggagatggc gctggcgcag gccttgcgcg gcgacgtgtt
     8401 cgaccacgtg gtggtcaacc gcgtcgtcgg ggaggcggcc gaggagatcc tccgtatact
     8461 ggatggaccc tccgcgactc ccgcgtgagt gccccctgcg tcaagaggtg agtcccatcg
     8521 ccaggatcga cgacctgctg aacaaggtgg acagcaagta cacgctggtc caccttgccg
     8581 cccgccgcgc ccgcgagatc aacagctact accactcgct gggtgagggc ctcggccagt
     8641 tcactccgcc cctggtcgac caggtggact ccaacaagcc gctgtccatc gccctggagg
     8701 agatcgccga ggcgaagatc gtcgtggccg agggccgtga cgcgctcgcc atggccgagg
     8761 agctgctcgg cggcggcgcc gacaccggcg cccaggtcgt ctcgctgtcc gacgaggacg
     8821 gg
//
LOCUS       DATMKC010000143         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_124859, whole genome shotgun sequence.
ACCESSION   DATMKC010000143 DATMKC010000000
VERSION     DATMKC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124859"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VNU01_04285"
     CDS             complement(<1..697)
                     /locus_tag="VNU01_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH31868.1"
                     /translation="MSGNLNRGRVAVAGVVMQVALGAVYAWSVFRKPLTEEYDTSVAA
                     VNTTFSITILALGFAAFAGGLWMAKVGPRTVALTAGVLYGAGIILASLAGDSLTLLYL
                     TYGLLGGIGIGLGYIVPIATLVKWFPDRRGFITGVAVAGFGGGALVTAPIARILVRDV
                     GLFETFAILGVVYLVMVAGAAMFLRNPPEGWSPEGWTPPKEGEQAAGRSGIDFDLRGA
                     LKRWQWYALWGILF"
     gene            897..>2753
                     /locus_tag="VNU01_04290"
     CDS             897..>2753
                     /locus_tag="VNU01_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005198162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1404..1406,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWH31869.1"
                     /translation="MDWGMGPAAASPGTRAAGTRIAGLKVSESVCPYCAVGCGQLVYT
                     RNGELVDIEGNPRSPINQGTLCPKGAASRQLIQQPGRLSKVKYRRAGGTEWEELDLDE
                     AMDMIAERLIAARENGWQDVDRNGWKVDRTLGFAHLGGATLDNEENYLIKKLFTAMGA
                     IQIENQARIUHSSTVPGLGTTFGRGGATNFQQDLANADCILIEGSSMAEAHPVGFRWV
                     VKAKERGARIIHVDPRYSRTSQIADQHVPIRAGSDIVFLGALIRHVIEEEAYFREYVV
                     NYTNAPTILREDFQDTEDLGGLFSGWDPETATYDTSTWQYENGGGQAEADDQTHTSYG
                     FEERSGAGMDVGGVPRDETLQHPRCVFQVLKRHFARYTPEMVERVCGIPREEFLEVAR
                     ALVENSGRERTTAFCYAVGWTQHTAGVQMIRTAAILQLLLGNMGRPGGGIMAMRGHAS
                     IQGSTDIPTLFDILPGYLKMPRAAEEEPSLADYVKAGGKERGWWAFYETYFVSLLKAW
                     FGDAATEDNEWGFRHLPKLTGNHSHFSTMLRALDGGVEGLFLMGQNPAVGSQHAGLQR
                     RALASLKWLVVRELAEIESATFWRDSPEVKSGELRTEDIQTEVFLMPAARGPC"
BASE COUNT          443 a          983 c          972 g          355 t
ORIGIN      
        1 ggaacaggat cccccacagc gcgtaccact gccagcgctt cagcgcgccg cgcaggtcga
       61 agtcgatgcc cgaccggccc gccgcctgct cgccctcctt cggcggcgtc catccctcag
      121 ggctccagcc ctcgggcggg ttgcgcagga acatcgccgc gccggccacc atcaccaggt
      181 agacgacgcc gaggatggcg aaggtctcga acagccccac gtcgcgcacg aggatgcggg
      241 cgatcggagc cgtgaccagc gcgccgccgc cgaagccggc caccgccacg ccggtgatga
      301 acccgcggcg gtccgggaac cacttgacca gcgtcgcgat cgggacgatg tagccgaggc
      361 cgatgccgat gccgccgagc aggccgtagg tcaggtacag cagcgtgagg ctgtcgccgg
      421 ccaggctggc gaggatgatc ccggcgccgt agaggacgcc cgccgtgaga gccaccgtcc
      481 gcggccccac cttcgccatc cacaggccgc ctgcgaaggc ggcgaagccg agcgccagga
      541 tcgtgatgga gaacgtcgtg ttgaccgccg cgacgctcgt gtcgtactcc tcggtcagcg
      601 gcttgcggaa cacgctccag gcgtagacgg cgccgagcgc cacctgcatg acgacgccgg
      661 cgacggcgac ccttccgcga ttcaggttcc cactcatgcc tctccctccc ggtccgccgc
      721 cccactaccc ggcccacggc cgtcgtcaat ccaggtgtgc cgggtccgcg cgcgggtacg
      781 tatggcggcg aggaaggaag gctgcgctga gccccggaaa cggatcgtcg cgcgaccccg
      841 cgtcgcggcc gcgctcattc gagcgactcc ggcggctgcc cgggatcggg cgcgcgctgg
      901 actggggcat gggccccgcc gccgcctcgc ccggcacgcg cgcggccggc acccgcatcg
      961 cggggctgaa ggtctcggaa tccgtctgcc cgtactgcgc cgtcggctgc ggccagctcg
     1021 tctacacgcg caacggcgag ctggtggaca tcgagggcaa cccgcgctcg ccgatcaacc
     1081 agggcacgct gtgcccgaag ggcgcggcgt cgcgccagct catccagcag cccggccggc
     1141 tgagcaaggt caagtaccgg cgcgccggcg ggacggagtg ggaggagctc gacctcgacg
     1201 aggcgatgga catgatcgcc gagcgcctga tcgcggcgcg cgagaacggg tggcaggacg
     1261 tcgaccgcaa cggctggaag gtcgaccgca cgctcggctt cgcgcacctc ggcggcgcca
     1321 cgctggacaa cgaggagaac tacctcatca agaagctgtt cacggcgatg ggcgccatcc
     1381 agatcgagaa ccaggcgcgc atatgacact cctccacggt ccccggtctg gggaccacgt
     1441 tcgggcgcgg cggcgccacc aacttccagc aggacctcgc caacgctgac tgcatcctga
     1501 tcgagggctc ctcgatggcc gaggcgcacc ccgtgggctt ccgctgggtg gtcaaggcca
     1561 aggagcgcgg ggcgcggatc atccacgtcg acccgcgcta ctcgcgcacg agccagatcg
     1621 ccgaccagca cgtcccgatc cgcgcgggca gcgacatcgt cttcctcggc gcgctgatcc
     1681 ggcacgtcat cgaggaggag gcgtacttcc gcgagtacgt cgtcaactac accaacgcgc
     1741 cgaccatcct gcgcgaggac ttccaggaca ccgaggacct cggcggcctg ttcagcggct
     1801 gggatcccga gaccgccacc tacgacacgt cgacctggca gtacgagaac ggcggggggc
     1861 aggccgaggc ggacgaccag acgcacacga gctacggctt cgaagagcgg tcgggcgcgg
     1921 gcatggacgt cggcggggtg ccgcgcgacg agacgctgca gcacccgcgc tgcgtcttcc
     1981 aggtgctcaa gcgccacttc gcccgctaca cgcccgagat ggtcgagcgc gtgtgcggca
     2041 tcccgcggga ggagttcctc gaggtcgcac gcgcgctggt ggagaactcg ggccgcgagc
     2101 gcaccacggc cttctgctac gcggtcggct ggacgcagca cacggccggc gtgcagatga
     2161 tccggaccgc cgccatcctg cagctgctgc tgggcaacat gggccgcccg ggcggcggga
     2221 tcatggcgat gcgcgggcac gcgtcgatcc agggctcgac ggacatcccg acgctgttcg
     2281 acatcctgcc gggctacctg aagatgccgc gcgcggcgga ggaggagccg tcgctggccg
     2341 actacgtcaa ggcgggcggc aaggagcgcg gctggtgggc gttctacgag acctacttcg
     2401 tctcgctgct gaaggcgtgg ttcggcgacg ccgccaccga ggacaacgag tggggcttcc
     2461 gccacctgcc gaagctcacc ggcaaccact cgcacttctc gacgatgctg cgggcgctcg
     2521 acggcggggt cgagggcctg ttcctgatgg gccagaaccc ggcggtcggc tcgcagcacg
     2581 ccgggctgca gcggcgcgcg ctcgccagcc tgaagtggct cgtcgtgcgc gagctcgcgg
     2641 agatcgagtc ggccacgttc tggcgcgaca gcccggaggt caagtcgggc gagctgcgca
     2701 cggaggacat ccagaccgag gtcttcctga tgccggcggc tcgagggcct tgt
//
LOCUS       DATMKC010000144         9659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_58929, whole genome shotgun sequence.
ACCESSION   DATMKC010000144 DATMKC010000000
VERSION     DATMKC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58929"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1765
                     /locus_tag="VNU01_04295"
     CDS             188..1765
                     /locus_tag="VNU01_04295"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HWH31870.1"
                     /translation="MDLLFAGGIGTYVRARGERDEDIGDRANDGVRVQADELRARVIC
                     EGANLSVTQAARIQYARRGGRVNQDAIDNAAGVAISDREVNAKILLRGVAGCDSAEVA
                     RLLAASEADVVAGVLADVGLQSACLTAEQRGGAARAASYERLMAELEAAGRLDREEEL
                     LPSTAEMASRAERGVALLRPELATLLAYAKRRLVAAIVDSPLPDQPVLAEELRRYFPP
                     ALVERAGPALDAHPLRRDLIATRLANDLVNRLGVTFAFDLAADMGVSPSVVAAAYWVA
                     RDVAGATRWWSMLLAREDDLGLDRVLELQERLDSLVAALVRGLVADPAGTRPLLEDTG
                     SVLARDAGGFAALLAADVGTSRRRRERRDTAGRLTTELGDAALAAVLAGVEELAYAPE
                     ILRVARTSRRAPAEVAEAFFLLGDHLGTPRLVRLARRAEAAPGEWADRQRRGLEEDIA
                     VAWLAAVADALGGAPEEAVRDALRRFADSREAAVESARDVLSQVEHEQEPTLDMVAVA
                     VRALTRAVASPAPEDGS"
     gene            1812..1888
                     /locus_tag="VNU01_04300"
     tRNA            1812..1888
                     /locus_tag="VNU01_04300"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1846..1848,aa:Met,seq:cat)
     gene            complement(2420..3649)
                     /locus_tag="VNU01_04305"
     CDS             complement(2420..3649)
                     /locus_tag="VNU01_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31871.1"
                     /translation="MSELLIHATHNEHRVVADLLAPRGSWRTEAPMARLVVDAVTALG
                     QEEFAKAAGDNGVPLVVDPMTLPLPHGAHPADSWMQLPYARAEGRTALSLRDPAARWE
                     LVSQTVELQIDLGATMLVPAHVYATSPGDPWHLINLALLRDTAQYVAEHHAGMSLLPV
                     LCGQADGFTRVASWRDGVDRFADMAASVGAGTVAVSMSPVGGVASDKYDKTLRYFSTL
                     LHLQQRGVRVIAWHQGVFGAGLIAAGVHGYSTGIGKLETSKAATQARGRLRGRSDDGD
                     GGSYKGLYLDAFGHSVPFHAGERLLHHEVLRPQLMCADDEGCCASWSETLEHPREHAV
                     RARARQRALLDGQPRSTWRLTQLVREAESAVNVAKQANQVLEAAGEKFRVKLVQLESL
                     RDVAEHLRQVAIGAATA"
     gene            3756..4115
                     /locus_tag="VNU01_04310"
     CDS             3756..4115
                     /locus_tag="VNU01_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH31872.1"
                     /translation="MSAIARETPGSSYQHLVNEIRRVSRMSAAELADLTGVRERQVHN
                     WAAGVSRPQDDSRDRLVDVHYIVKTLSEIYVPEGVEIWLHGRNRGLGGRRPIELLLHG
                     DFEPVVREVESLNSGAM"
     gene            4139..4705
                     /locus_tag="VNU01_04315"
     CDS             4139..4705
                     /locus_tag="VNU01_04315"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWH31873.1"
                     /translation="MARVPRTTVEGTFYRHAHVGTRDLRGSNAGGRWGPPGGFEVLYL
                     GRPEPSVVIEAHRHLVEAVEGMRPDLVGPRALIAVAVAVGNVADLRDAAARVQLGLPD
                     EVIFSAARDPDAYQECRLVARAAHQLELHGIIAPAAGRFGGETLALFPAHLPVAEQPR
                     IIDVAIWSTLPADPRQLRALQRTEADEG"
     gene            5293..6396
                     /locus_tag="VNU01_04320"
     CDS             5293..6396
                     /locus_tag="VNU01_04320"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH31874.1"
                     /translation="MEGGFDFVLRERVSASNLPFAGAPPIREHDLAVGLLPIRYLALA
                     HVIQALHTTGLADLLAGKALTVEDLAVQLELDPDRLQHLLYYLINEGLVEVDAGGRYE
                     MSSRGQAYVTCRPWYELLVGGYAQTFSELEQSLRTSQHYAQRNDSRVGKGSCLISFHD
                     ALPMAARLLATCEPEPSLIVDLGCGDGQFLLELCQAMGLRGVGIEQAAASVARAQDMI
                     TGLGLDQQVTVVQGDALRSLSEYPEERPCYLAAFVLQELLEQRSREEVVDLLRRSAKL
                     NPEAAWIVVEVDHRPDDERLQSNLGLAYYNPYYLLHAITCQRLETREFWVDVAAEAGF
                     DIAASAVPEPSYDSLGLKFALLLRPAGRPEENT"
     gene            6393..7577
                     /locus_tag="VNU01_04325"
     CDS             6393..7577
                     /locus_tag="VNU01_04325"
                     /inference="COORDINATES: protein motif:HMM:NF005732.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HWH31875.1"
                     /translation="MIRPTVADMPTSPILSLGRTAEGVLASQDVIDLTQGVADWSPPP
                     AVQERLRVIVHEPDAWGYTARAGAPDVRLAIAEDSADLYGAPLTPDHVLVTGGCNQAF
                     CVAVATVAGPGDEVVLFSPFYFNHPSCLRLLGIRPVVQPLLPGGQPCVESLQQAIGSR
                     TRAVVVVTPGNPTGSTISGQALRDLSAMARHHGLLLILDETYRRFAFDDGSRPHDLHS
                     DPDWEGSLVSLLSFSKELAMPGQRVGALLAAPAFVEQAVKVLDAISVCAPRYGQEAVA
                     AGLKASGAWMEERRRLVSNRRESLVDALSSHPGGFSVESAGGFFAWVSHPFQDRSDVE
                     ICGRLAEVVGLSVLPGSAFALGETGCIRLSYASLPAERAMLVAERLARFAQRNRRSEA
                     GR"
     gene            7574..8728
                     /locus_tag="VNU01_04330"
     CDS             7574..8728
                     /locus_tag="VNU01_04330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH31876.1"
                     /translation="MSPSVSGTYDVVIIGAGVQGLAAAYNLARGGCRRVLVLEREARP
                     GMGSSARSGSMLMKSRENAPKVALSLYSYERYMTFSDEFGEPLQFRRTGFVSAVPPDL
                     ADRYLAMHRTRTDMGVPSEVVDPSALSKMSPALRVEDLAFGLYCPDDGEIDVAQIVGA
                     FARHATALGVELATGETAVGLEVRGGRVTAVRTTRGVYPCAVVVNAAGADAAEVAAWA
                     GLSLPIDNRRRSIFLGTCEDPRLQVGPMVEDAAVEWYFRGLGGRRVLVGMGKEPTGAP
                     GDEPDWAMLPDVRRVAAHRAPTLERIEVVAGSSGIRSLTPDLLPILGPAEGVDGMILS
                     CGWGGEGIMHAPAGGAVVADTVLGSRSYPYDRDEFMLSRFATQSTSGGYE"
     gene            8725..9174
                     /locus_tag="VNU01_04335"
     CDS             8725..9174
                     /locus_tag="VNU01_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWH31877.1"
                     /translation="MNVKDRLNELGYEYSPAPVEVLTFHSAVRHGSLVYTSGQIPLLG
                     DMAIKGKVGAEVDLGMAQKAAEICAFNCLRAVGAVCDIESVERVVKVLGMVNTAPGFN
                     DTSSVINGCSEFLTRVFGDRGFHARSAVGMVLPGDWAVEVEMVVAVS"
     gene            9171..>9659
                     /locus_tag="VNU01_04340"
     CDS             9171..>9659
                     /locus_tag="VNU01_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31878.1"
                     /translation="MTDIVSPMPATPGEVLDVRPVCQVVIGDFGRDAAVRYLSALSRS
                     ERRFLQGVLAPCGLPFFDEYRRITWVALAEVAFIKAELIESVRRRDGHLTDQVLLGEA
                     WSAVPESVMEALVELVMTAVAAVVSRGYERVRVVLPCNSLGGMLTEVGRRVVGRLSAD
                     GLD"
BASE COUNT         1423 a         3056 c         3500 g         1680 t
ORIGIN      
        1 gcgcctgcag caccgcgcgg atcagctccg gcggcgtcat cgcctccgcg tccacgcgca
       61 gcagcgcgcg cacctgcggc gacagcggga tgcgcttggc cgcgcgcgag aacaccccgc
      121 cgcccgggct gatcgcggag cggtcgtagt cctgccagct ggagcggggc agcgcctgca
      181 ggcgccggtg gacctgctgt tcgccggggg catcggcacg tacgtgcggg cgcgcgggga
      241 gcgcgacgag gacatcggcg accgcgccaa cgacggcgtg cgcgtgcagg ccgacgagct
      301 gcgcgcgcgc gtgatctgcg agggcgcgaa cctgtccgtc acgcaggccg cgcgcatcca
      361 gtacgcgcgg cggggcgggc gcgtcaacca ggacgccatc gacaacgcgg ccggcgtggc
      421 gatctccgac cgcgaggtca acgccaagat cctgctgcgc ggggtggccg gctgcgacag
      481 cgcggaggtg gcgcggctgc tggccgcctc ggaggccgac gtggtggcgg gcgtcctggc
      541 cgacgtcggg ctgcagtcgg cgtgcctgac cgccgagcag cgtggcggcg cggcgcgcgc
      601 cgcctcctac gaacggctca tggcggagct ggaggcggcg gggcggctgg accgcgagga
      661 ggagctgctg ccgtccaccg cggagatggc ctcgcgcgcc gagcgcggcg tcgcgctgct
      721 gcgcccggag ctggcgacgc tgctggcgta cgccaagcgg cggctggtgg ccgcgatcgt
      781 ggactcgccg ctgccggacc agccggtgct ggccgaggag ctgcgccgct acttcccgcc
      841 ggcgctggtg gagcgcgccg gtccggcgct ggacgcgcac ccgctgcggc gcgacctgat
      901 cgccacgcgc ctggccaacg acctggtgaa ccgcctgggg gtgacgttcg ccttcgacct
      961 ggccgccgac atgggggtgt cgccgtcggt cgtcgcggcg gcgtactggg tggcgcgcga
     1021 tgtggccggt gcgacgcggt ggtggtccat gctgctggcg cgcgaggacg acctggggct
     1081 ggaccgcgtc ctggagctgc aggagcgcct ggacagcctg gtcgcggcgc tggtgcgcgg
     1141 cctggtcgca gaccccgccg gcacacgccc gctgctggag gacaccggca gcgtgctggc
     1201 gcgcgacgcc ggcggcttcg cggcgctgct cgccgccgac gtcggcacgt cgcggcggcg
     1261 gcgcgagcgg cgcgacaccg ccgggcggct gaccaccgag ctgggcgacg cagcccttgc
     1321 ggccgtgctg gccggcgtgg aggagctggc gtacgcgccg gagatcctgc gcgtggctcg
     1381 cacgagccgg cgcgcccccg ccgaggtggc cgaggcgttc ttcctgctgg gcgaccacct
     1441 gggcacgccg cgtctggtcc ggctggcccg gcgagccgag gcggcgcccg gtgagtgggc
     1501 cgaccggcag cgccggggct tggaggagga catcgccgtg gcgtggctgg cggcggtggc
     1561 cgacgcgctg ggcggcgcgc cggaggaggc ggtgcgcgac gcgctgcggc gcttcgccga
     1621 cagccgggag gcggccgtgg agtccgcgcg cgacgtgctg tcgcaggtgg agcacgagca
     1681 ggagccgacc ctggacatgg tggccgtggc ggtgcgcgcg ctgacgcgcg cggtggcctc
     1741 gccggcgccc gaggacggtt cctgacggcc gtggctttgc acggcttgtg gggcactgct
     1801 accgtttctg gcgcgaggtg gagcagttcg gtagctcgcc gggctcataa cccggaggtc
     1861 gcaggttcaa atcctgccct cgcaaccaag agaacccctg ctacggcagg ggttctcgcc
     1921 tatgggggca tcgacggagt tcgatgggtc atccacgggt cacgctttca tcaggcctct
     1981 gacctgcgga aacatggaca ccgcgagcgg gctttgtctt gcggagcgtg tgtcggtctg
     2041 tccggccggg tcacctgggg tcagggaagg gcgctgggcg ccaccactcg aggagggtcc
     2101 ggaatatctg ttttgtgggc cttggttctg cgacgccttg tgaccaattc tgagtgatgt
     2161 tgcgcggcca gcggcaccag ccagcgcgct gaccagcggt tttgtcaccg gaatgcggaa
     2221 ttcacctccg ggttccggca ttttcgaacc cggaggtccc cgtctgaggt tttgggggga
     2281 caagcgggca ggaacgccat aaacagcatg ttcaacgcat cacggatgtg attcgtcggt
     2341 atgggcgccg agggtgcatc tgcgccgcgg tggtacgtgc gggcggcgcc tgagccccgc
     2401 ttcggtgcct gtgctgtgat caggcggtcg ccgcgccgat cgcgacctgg cgcaggtgtt
     2461 cggcgacgtc gcgcagtgac tcgagctgca cgagcttgac tcgaaacttc tcgcctgcgg
     2521 cctccaggac ttggttggcc tgcttggcga cgttgactgc ggactcggcc tctcggacca
     2581 gctgggtgag ccgccaggtc gagcgcggct gcccgtcgag cagcgcgcgc tggcgtgcgc
     2641 gagcgcgcac ggcgtgctcg cggggatgct ccagggtctc tgaccacgag gcgcagcagc
     2701 cctcgtcgtc ggcgcacatc aactgcgggc gcagcacctc gtggtgcaga aggcgttccc
     2761 cggcatggaa cgggacgctg tggccgaagg cgtctaggta cagccccttg taggagccgc
     2821 cgtctccgtc gtcggacctt ccgcgcaacc ggccgcgcgc ctgggtcgcc gccttgctgg
     2881 tctcgagctt gccgatgccg gtggagtagc cgtgcacgcc cgcggcgatc aggcccgcgc
     2941 cgaacacgcc ctggtgccag gcgatcacgc gcacaccgcg ctgctgcagg tgcagcaggg
     3001 tgctgaagta gcgcagggtc ttgtcgtact tgtcggaggc gacgccaccc accggtgaca
     3061 tcgacacggc gaccgtcccc gccccgacgg aggcggccat gtcggcgaag cggtcgacgc
     3121 cgtcgcgcca cgaggcgacg cgtgtgaacc cgtcggcctg tccgcacagc acgggcagca
     3181 ggctcatgcc cgcgtgatgc tcggcgacgt actgggccgt gtcgcgcagc agcgccaggt
     3241 tgatcaggtg ccacgggtcg ccgggggacg tggcgtagac gtgcgccggc acgagcatcg
     3301 tggcgcccag gtcgatctgc agctcgacgg tctggctgac cagttcccag cgggcggcgg
     3361 ggtcgcgcag tgacagggcg gtgcggccct cggcccgagc gtagggcagc tgcatccagc
     3421 tgtcggccgg atgcgcgccg tgcggcagcg gcagggtcat cgggtccacc accagtggca
     3481 ctccgttgtc accggccgcc ttggcgaact cctcctgtcc gagtgcggtc accgcgtcca
     3541 cgaccaaccg cgccatgggg gcctcggtgc gccaggagcc cctgggggcc aggagatcgg
     3601 ccacgacgcg gtgttcgttg tgcgtggcgt ggatgagcag ctcggacact gtgggcctct
     3661 tgacacgctg gacgtgtgcc accgttcgcg ctatggtacc tgcaacaacg tctgcaatct
     3721 agtctgtaac aaagactgtc gaaagaggtg cgcgaatgtc ggcaatcgcg agggagacgc
     3781 cagggagctc ctaccagcac ctcgtcaacg agatccggcg ggtgagccgg atgagcgcgg
     3841 cggagctcgc cgacctcacc ggggtgcgcg aacgccaggt gcacaactgg gcggccggcg
     3901 tgtcgcgacc tcaggacgac agccgcgacc gactggtgga cgtgcactac atcgtcaaga
     3961 cgttgagcga gatctacgtg cccgagggcg tggagatctg gctgcacggc cgcaatcgcg
     4021 ggctgggcgg acgccgcccg atcgagctgc tcctgcacgg ggacttcgag ccggtggtcc
     4081 gcgaggtcga gagcctcaac agcggggcga tgtgacgcgg cgcgacctgg tcgcacgcgt
     4141 ggcacgcgtg cccaggacca ccgtcgaagg gacgttctac cgtcacgccc acgtcggcac
     4201 ccgcgacctt cgcgggtcca acgccggagg gcggtggggt ccgcccggcg gcttcgaggt
     4261 gctgtacttg ggccgcccgg agccctccgt cgtgatcgaa gcccaccgcc atctggtgga
     4321 ggccgttgaa ggcatgcgac cggacctcgt cgggccgcgg gcgctcatcg ccgtggccgt
     4381 cgccgtcggc aacgtcgcgg acctgcgtga cgccgcggca cgcgtccagc tgggcctgcc
     4441 tgacgaggtg atcttcagcg ccgcacgcga tcccgacgcc taccaggagt gccgcctcgt
     4501 cgcccgagcc gcccaccagc tcgagctgca cggcatcatc gccccggcag cgggccgctt
     4561 cggtggcgag accctcgctc tgtttccggc gcacttgccc gtcgccgagc agccacgcat
     4621 catcgacgtc gcgatctgga gcaccctgcc cgccgatccc cggcagttgc gtgcgcttca
     4681 gcggaccgag gccgatgagg ggtagccctg gacggcgacg acccgagcgg atccacgcgg
     4741 ttccgcgcca ggtctcattg cccggcgtga ggggcctgca cagggcggcg tcccagtcgt
     4801 gtgcgacgtg gtcgctgagc gggatggaca ggcgatcggc catagcaggc atccaggtcg
     4861 atggtgctgg ccagacgctg cacgacggcg atgcgacgag cacctcgctg cggcgcatgc
     4921 cagtcatggt tgcgagcagc cacagttggt acgggcactg gtgcagttgc tggcggtcca
     4981 cacgaggcgg tcgcgtcggc tgccggcggt ggcctgctgc aggatggacg gtgtggcgcc
     5041 gtcgacgggg ttcttggcct gcagccccga gcgcacggcg tcgctgaacg gcttgtacag
     5101 cttggcgtgg acctcacgga cgcttttggg gtgacagggg ccgcttcgac cgtccgcgca
     5161 accgtgatcg gacaggtccg tgtacggcga tgggcactag tggcagcaca cattcactac
     5221 cagacggttc cgtcggctac tatgttggcc tgcacgtggg cgcggggcgt gccggatcga
     5281 ttggaggggc cattggaggg gggcttcgat tttgtgttgc gcgagagggt aagcgccagc
     5341 aatttaccgt ttgccggagc gccgcccatt cgtgaacacg atctggctgt gggcctgcta
     5401 cccattcggt acttggcgct ggcgcatgta atccaagccc tgcacaccac cggtctggcg
     5461 gacctcctgg caggcaaggc gttgactgtc gaagacctcg cagtgcaact ggagctggat
     5521 ccggaccgac tccagcacct cctgtactac ctcatcaatg aggggttggt cgaggtcgac
     5581 gcgggcgggc gctatgagat gagcagtaga gggcaagcgt acgtgacgtg tcggccctgg
     5641 tatgaactgc tcgttggcgg ctatgcgcag acatttagcg agttggagca atctcttcgc
     5701 acgtctcaac attacgccca gcgcaacgac agtcgcgtcg gcaaggggag ctgtctcata
     5761 agctttcacg acgccctccc tatggccgcg cgcttgcttg caacctgcga gccggagcca
     5821 tcgcttatcg tcgatctggg gtgcggcgac gggcagttcc tgctcgaact ctgccaagcc
     5881 atgggtctcc gcggtgtggg gatcgagcag gcagcggcga gcgtcgcgcg ggcccaggac
     5941 atgatcacgg gtctgggttt ggatcaacag gtgaccgtcg tgcaagggga cgcattgcgg
     6001 tccctatccg aatatcctga ggaacgcccg tgctatttgg cggccttcgt cctccaagag
     6061 ctcttggagc agcggagtcg agaggaggtg gtcgatctcc tacggcggtc cgcaaaatta
     6121 aatcccgaag cggcctggat cgttgtggag gtcgatcacc gtccggacga cgaacggctt
     6181 cagagcaacc tagggctggc ttactacaat ccgtattatt tgttgcacgc tataacgtgc
     6241 caacggctcg aaacccggga gttttgggtt gatgtcgcgg ccgaggccgg atttgatatt
     6301 gccgccagcg ccgtgccaga accctcgtac gactccctgg ggctgaagtt cgcgctcctc
     6361 ttaaggcctg ccggacgtcc tgaggaaaat acgtgattcg ccccaccgtt gcggacatgc
     6421 cgacgtcccc gatcctgtct ctgggacgga cagcggaagg ggttctagcg tcccaggacg
     6481 tgatcgatct gacgcagggg gtagcggact ggtcaccgcc gccggcagtc caggagcgcc
     6541 tgcgcgtaat agtgcatgaa ccggacgcct ggggatacac cgcgcgggcg ggggcacctg
     6601 atgttcgcct ggcaatagct gaggacagtg ccgacctcta cggtgccccg ctcaccccag
     6661 accacgtgct ggtgaccggc ggatgcaatc aggcattctg cgtggcggtt gcgacggtcg
     6721 caggtcccgg tgacgaagtc gtccttttct cgccgttcta tttcaaccat ccgtcgtgcc
     6781 tccgcctgtt ggggatacga cccgtggtcc aacccttgtt gcctggtggg cagccgtgcg
     6841 tcgaatcgct gcagcaggcg attggttcgc gcacgcgtgc cgtcgtcgtt gtgacgcccg
     6901 gcaaccccac tgggagcacg atcagcggcc aggcgctgcg tgacctctcg gctatggcgc
     6961 ggcatcacgg cctcctgctc atcctggacg agacctatcg acggttcgca ttcgatgacg
     7021 gttcgcgacc gcacgatctt catagcgatc cagactggga gggttctttg gtgagcctgc
     7081 tctccttctc gaaagagttg gccatgccag gccagcgggt cggagcgctc ctcgccgccc
     7141 ccgccttcgt tgagcaggcc gtcaaggtgc tggacgctat ttccgtctgc gctcctcgct
     7201 atgggcagga ggctgtcgcg gcgggtctga aggcctccgg ggcctggatg gaggaacgcc
     7261 gtcggctcgt gtcgaaccgg cgggagagcc tcgtcgatgc attgtcttcg caccccggtg
     7321 gcttctccgt cgaatccgct ggcggcttct ttgcctgggt cagccatccg tttcaggaca
     7381 ggtcggatgt agaaatttgc ggacgccttg cggaagtcgt ggggctttcc gttttgccag
     7441 gtagtgcctt cgctctgggt gagaccgggt gtatccgtct gagctacgca tccctgccag
     7501 cggagcgagc gatgctcgtc gccgaacgcc tcgcgcgatt cgcgcagcga aaccgacgct
     7561 cggaggcagg ccgatgagcc cgtcggtgtc gggtacatac gacgtagtca tcatcggagc
     7621 gggagtccag ggactcgcgg cggcgtacaa ccttgcacgg ggaggctgcc gccgcgtctt
     7681 ggtgttggag agggaagccc gcccgggcat gggatcgtcc gcaaggtccg gctcgatgct
     7741 catgaagtca cgggagaacg ctcccaaggt ggcgttgtcg ctctactcat atgagcggta
     7801 catgactttc tccgacgaat tcggagaacc attgcagttt cgtcgcaccg gttttgtgag
     7861 cgcggtgccg cccgacctgg ccgatcgtta ccttgccatg caccggacgc gtacggacat
     7921 gggtgttccc tccgaggtcg tggatccgtc cgccctcagc aagatgtcgc cagctctccg
     7981 cgtggaggat cttgcattcg gtctgtactg cccggatgac ggagagatcg atgtggcaca
     8041 aatcgtcgga gcgttcgccc gacacgcgac tgcacttggc gttgaactgg cgacggggga
     8101 gacggcggtc ggccttgagg ttcgcggcgg ccgcgtgacc gcggtgcgca cgacgcgtgg
     8161 cgtctatccg tgcgcggttg ttgttaatgc cgccggcgcc gatgccgcag aggtcgcggc
     8221 atgggcggga ctgtcgctcc cgatcgacaa tcgacgtcgt tcgatttttc tggggacgtg
     8281 tgaggatccg cggctgcagg ttggcccgat ggtcgaggac gccgcagtgg agtggtattt
     8341 ccgtggactt ggtggacgtc gtgtgctggt tggaatgggc aaggagccta caggagcccc
     8401 gggggacgaa cccgattggg ccatgctgcc cgacgtccgt cgcgttgccg ctcaccgcgc
     8461 accgacattg gagcggattg aagtggttgc gggctccagc ggtatccgct cgctgacccc
     8521 ggatctgctg ccaatcctgg gccctgcgga gggcgttgat gggatgatcc tcagctgcgg
     8581 ctggggcggt gaaggaatca tgcacgcacc agccggggga gccgtggtag ccgacaccgt
     8641 tcttggttcc cgcagctacc cctacgaccg cgatgagttc atgttgtcca gattcgcaac
     8701 gcaatcaacc tcaggaggct acgaatgaac gtgaaggacc gtctgaacga gctgggatac
     8761 gagtactccc ctgcgcctgt ggaggtgctt actttccatt cagcagtgcg ccacggctcc
     8821 ttggtgtaca cgtcaggcca gatcccgctc ctcggcgata tggcgatcaa ggggaaggtg
     8881 ggagccgagg tcgacttggg aatggcgcag aaggcggcgg agatttgcgc gttcaactgt
     8941 ttgcgcgccg tcggggccgt gtgcgacatc gaatccgtgg agcgtgtggt caaggtcctg
     9001 ggcatggtga acacggcacc cggtttcaac gatacgtcaa gcgtcatcaa cgggtgcagc
     9061 gagttcctga cgagggtgtt cggcgatcgt ggattccatg cgcgctcagc tgtcggcatg
     9121 gtcctgcccg gcgattgggc ggtggaggtg gagatggtag tcgccgtctc atgaccgaca
     9181 tcgtctcgcc tatgccggct acaccgggcg aggtgctcga cgttcggccc gtctgccaag
     9241 tggtgatcgg ggactttgga cgagacgccg ccgtgcgcta cctatccgca ctcagccggt
     9301 cggaacgtcg cttccttcaa ggcgtgctcg caccttgcgg gctgcccttc ttcgatgagt
     9361 accgccggat cacctgggtg gcgctcgcgg aggtcgcctt cattaaggcc gagcttatcg
     9421 agagcgtgcg acgccgcgac ggtcatctga ccgatcaggt gctgctcggt gaggcgtggt
     9481 cggctgtgcc ggagtcggta atggaagcgc tcgtggaact ggtcatgacc gcagtggccg
     9541 ccgtcgtttc acgtggttac gagcgtgtgc gggtcgttct gccatgcaac agcctcgggg
     9601 gaatgttgac cgaggtcggt cggcgtgtcg tcggacggtt gtctgcggac ggcctggac
//
LOCUS       DATMKC010000145         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_52040, whole genome shotgun sequence.
ACCESSION   DATMKC010000145 DATMKC010000000
VERSION     DATMKC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52040"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VNU01_04345"
     CDS             complement(<1..238)
                     /locus_tag="VNU01_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31879.1"
                     /translation="MSAPQPVLERLRRRVAEDPSVAAAGAAQGSVRRLEVVRGAVARA
                     VREEGVLLPAVELTALVRELADTLAGLGPVEGLLR"
     gene            complement(235..1149)
                     /locus_tag="VNU01_04350"
     CDS             complement(235..1149)
                     /locus_tag="VNU01_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31880.1"
                     /translation="MDTGDGELPVAVELPAGLELDVTLFVEREAGWRRVPLEGPPSPA
                     LLLTQRARTWPPSVVVLPGPVDAATVRRALLEGALDVIAWPEDRERLAACAGRLPAGP
                     SAPAPLVLSVVAASGGVGASTVALALAGLVAWGGRRVAITGGEGMLRLAGLSPWRGPG
                     ADELARLAPGEALREFPRVAHAVPGVRGLWALHASGSPAAHGWPVEVLVVDGAVTGPA
                     PAHGAVLAVARADAGLERLAGAAVPVVVNGAGPRSAAQVRRILGREPLAWLPHSARVA
                     RAACAGRVPAGLPASWLSPLRRAVGRLS"
     gene            1280..2104
                     /locus_tag="VNU01_04355"
     CDS             1280..2104
                     /locus_tag="VNU01_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWH31881.1"
                     /translation="MRPRQHRVAAFFDLDKTVVAKSSTLAFGKPLYKEGLITPAVVLK
                     GAYAQMAYQLLGADEKRMEKSRVALLELTKGWEAERVRRLVRETLQEIIDPLVYAEAL
                     ELFEEHKAAGRDLYLVSSSGEEVVVPLAEYLGVPHVIATRAGMDDAGCYDGTLDFYCY
                     AENKAIAIRAEAEAKGIDLDASFAYSDSVTDLPMLEAVGNPVAVNPDKELRKIAEERG
                     WRIMDFKRPVRLRDRFGNLPKPKTEVIAGAAGLGALALAGWALYQRRPGRPGADKA"
     gene            2107..2733
                     /locus_tag="VNU01_04360"
     CDS             2107..2733
                     /locus_tag="VNU01_04360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH31882.1"
                     /translation="MDAREAAAAALRRLGHAVVGHDCDPALLERIAAAAERIAADVEG
                     GTPRSRPIESMKRRLWESPPPDGGPMSHFAECVVSGEANPMGIAIQPRRVGDEVVADF
                     NLGAAFEGAPRRAHGGVVAAIFDDIMGYVLVLERTPAYTGQMTVDYLAPVPIGVDLQA
                     RAWMEARDGRKLRMRAEMTQDGAVIGRSTGLFIAIPPERLGITASSTA"
BASE COUNT          402 a         1159 c         1080 g          321 t
ORIGIN      
        1 cccgcagcag tccctccacc gggcccagcc cggccagggt gtcggccagc tcgcgcacca
       61 gcgcggtcag ctccacggcc ggcagcagca cgccttcctc gcgcaccgcg cgggccaccg
      121 cgccgcgcac gacctcgagc cgccgcacgc tgccctgcgc cgcccctgcc gccgccaccg
      181 acgggtcctc cgccacgcgc cgccgcaagc gctccaggac cggttggggc gcgctcacga
      241 cagccgcccc acggcgcggc gcagcggcga cagccacgag gcgggcagcc ccgccggcac
      301 ccggcccgcg cacgccgccc gagccacgcg cgccgagtgc gggagccagg ccagcggctc
      361 ccggcccagg atgcggcgca cctgcgccgc ggagcgcggc cccgcgccgt tgaccaccac
      421 cggcacggcc gcgccggcga ggcgctccag gccggcgtcc gcgcgcgcga cggccagcac
      481 ggcgccgtgc gcgggggccg gcccggtcac cgcgccgtcc accaccagga cctcgaccgg
      541 ccagccgtgc gcggcgggcg acccggacgc gtgcagcgcc cacaggcccc gcacgcccgg
      601 caccgcgtgc gccacgcggg gaaactcccg cagtgcctcg ccgggagcca ggcgcgcgag
      661 ctcgtccgcg cccggaccgc gccacgggga caggcccgcc agccgcagca tcccctcgcc
      721 gccggtgatg gccacgcgcc ggccgcccca cgccaccagg cccgcgagcg cgagcgccac
      781 cgtcgacgcg cccacgccac ccgacgcggc gacgacggac agcaccaggg gcgccggcgc
      841 cgacggccct gccggcagac ggccggcgca cgcggccagg cgctcgcggt cctccggcca
      901 cgcgatgacg tccagcgcgc cctccagcag cgcgcgccgc acggtcgccg cgtcgaccgg
      961 gccgggcagc accaccacgc tgggcggcca ggtcctcgcg cgctgggtca gcagcagcgc
     1021 cggcgacggc gggccctcca gcggcacgcg gcgccagccg gcctcgcgct ccacgaacag
     1081 cgtgacgtcc agctccaggc ccgcgggcag ctcgaccgcc acgggcagtt ccccgtcccc
     1141 cgtgtccacc gcgcctccgt catcgtcggg accgcgcacc ggtccggcgg ggcagcaacc
     1201 taagccgtga caggggggtg ccggcgaaga acgtgccctc cggctgtgga tgaaccgccg
     1261 ggacggttac gctcctgcca tgaggcctcg ccagcaccgt gtcgccgcgt tcttcgacct
     1321 ggacaagacc gtggtggcca agtcgtccac gctcgccttc ggcaaaccgc tgtacaagga
     1381 gggcctgatc acgccggcgg tggtcctcaa gggcgcctac gcgcaaatgg cctaccagct
     1441 gctcggcgcc gacgagaagc gcatggagaa gtcccgcgtg gctctgctgg agctcaccaa
     1501 gggctgggag gccgagcgcg tccggcgcct ggtgcgcgag acgctgcagg agatcatcga
     1561 cccgctggtc tacgccgagg cgctggagct gttcgaggag cacaaggcgg cgggccgcga
     1621 cctgtacctg gtcagctcct ccggcgagga ggtggtggtg ccgctggcgg agtacctcgg
     1681 cgtgccgcac gtcatcgcca cgcgcgccgg catggacgac gccgggtgct acgacggcac
     1741 gctggacttc tactgctacg ccgagaacaa ggccatcgcg atccgggcgg aggcggaggc
     1801 caagggcatc gacctggacg cgagtttcgc ctactccgac tcggtgacgg acctgcccat
     1861 gctggaggcg gtgggcaacc ccgtggcggt caacccggac aaggagctgc gcaagatcgc
     1921 cgaggagcgc ggctggcgga tcatggactt caagcgcccc gtgcggctgc gcgaccgctt
     1981 cggcaacctg cccaagccca agacggaggt catcgcgggc gccgccggtc tgggcgcgct
     2041 cgcgctcgcc ggctgggcgc tgtaccagcg ccgccccggg cggccgggag ccgacaaggc
     2101 gtgacggtgg acgcccgtga ggccgctgcg gccgcgctgc gccggctcgg ccacgccgtg
     2161 gtgggccacg actgcgaccc ggcgctgctg gagcgcatcg ccgccgccgc cgagcgcatc
     2221 gccgcggacg tggagggcgg cacgccgcgg tcgcggccca tcgagagcat gaagcgccgc
     2281 ctgtgggagt cgccgccgcc cgacggcggg cccatgagcc acttcgccga gtgcgtggtg
     2341 tccggcgagg cgaacccgat gggcatcgcg atccagccgc gccgcgtcgg cgacgaggtg
     2401 gtggcggact tcaacctcgg cgcggccttc gagggcgccc cgcgccgggc gcacggcggt
     2461 gtcgtcgcgg cgatcttcga cgacatcatg ggctacgtgc tggtgctgga gcgcaccccc
     2521 gcctacaccg gccagatgac ggtcgactac ctcgcgccgg tgccgatcgg ggtggacctg
     2581 caggcgcgcg cctggatgga ggcgcgcgac ggtcgcaagc tgcgcatgcg tgccgagatg
     2641 acgcaggacg gcgcggtgat cggccggtcg accggcctgt tcatcgcgat cccgccggag
     2701 cgcctcggca tcacggcgtc atccacagcc tgaccccttg tgcgggtgcg ctttcagagc
     2761 gatggctctg gaagtgcacc caacggggcg ccggctactg gtagtcgcgg tggtagcgga
     2821 tgcggccgcc gtccacctcg aaccagctcg cgcgcggcag atcgttccag cggcggccgc
     2881 cggccgtgta gctgatcgtc cactcgaccg cgatcatgga gccgtcctcg gactccagca
     2941 tgcgcttggt ggtgacgctg tc
//
LOCUS       DATMKC010000146         6600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_316277, whole genome shotgun sequence.
ACCESSION   DATMKC010000146 DATMKC010000000
VERSION     DATMKC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316277"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VNU01_04365"
     CDS             <1..472
                     /locus_tag="VNU01_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWH31883.1"
                     /translation="EGIPYSRSPEPRNPRLIAELYVGELGLDRPIPASQFDRDGGEVI
                     GGPAVLAPGGSAEMAGGNLTLEFGELRMWSGFQVSHAPGRGLLLAAAVLLLGGLVPSL
                     YSYRRRVWVEAHPDGEGTRVVLAGVALQRKATFADEFPRLAARVRAAVPPTDEA"
     gene            469..1626
                     /gene="ccsB"
                     /locus_tag="VNU01_04370"
     CDS             469..1626
                     /gene="ccsB"
                     /locus_tag="VNU01_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HWH31884.1"
                     /translation="MTTMVDEQLAAASNTLWNVAFVGYVVAMVCYFFRLAFTKVSTEG
                     VTVSTAAGRRVGMAGTAIAVAAAGVHTVSVVQRGLATGRVPWANMYEYSSLMALLAVV
                     AGLVVVQRRFGYGHLMGFVLALAVLSMTSALLLYAEPGPVVPALQSYWIKIHTSAAMA
                     SSSIFMVGFAATALYLVKDTAERRVAARSGASFGPGSVGAAEAGAQLAEGYEADTGVP
                     DADRVADATAQRDVLSPVLFPVLPFLVIGVFTLAVWQAPVAALLAGSVGALLGASSWY
                     AVPYLPAAARLDNLAYRVIAFAFPIWTFAVIAGAIWAEEAWGRYWGWDPKETGSFFTW
                     VLYASYLHARSTHGWRGRRAAWIAVVAFVALMITYYAVNLWVVGLHSYAGV"
     gene            complement(1673..1951)
                     /locus_tag="VNU01_04375"
     CDS             complement(1673..1951)
                     /locus_tag="VNU01_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31885.1"
                     /translation="MGIVRERDQGDEEEPGGSSPWVDLGLRVCPACRRELMGWEERCP
                     TDGAEGVRKGSVQADGTPPIPAHLLDDQPDPDVDLDADPDAHLSNRSQ"
     gene            complement(1969..3315)
                     /locus_tag="VNU01_04380"
     CDS             complement(1969..3315)
                     /locus_tag="VNU01_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH31886.1"
                     /translation="MANFVNRERELTALSRACGPERGPGGGMAVVSGRRRVGKTALLD
                     RFASGRDAPAIFLPGTRAPVAEALRRLEERVRAAVRPAAGDLLDLGHFDSWDAALGYL
                     IARGAEEPLLVVLDEFPYLCEADPSLPSTLQARWDHRGDSRLSVILAGSHVGLMEELV
                     AADAPLFGRADAHLRLAPFSWREAGLLVGGGDPAAWLEAYVTVGGMPRYLALWDPRHD
                     AVANLTELLDGPGAPLGDEGTVVLQELAAGSAPARILELVALGADTFGAIRERTGFAP
                     ATTSEALAALEGLGLLDRLTPVGEDPRRTKRVRYQVRDPLLRLWLGLVLPHREAFELG
                     RGGAVLQANRAALAAGQQAAFAAVARDWLGGVEAASCGPWWPTGDGEAVDALALRGTT
                     AAGAATARWATGVDGAAEVARLRAALAASPYGEAESLVVLARGGEGVTRPAELYEG"
     gene            complement(3287..3541)
                     /locus_tag="VNU01_04385"
     CDS             complement(3287..3541)
                     /locus_tag="VNU01_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31887.1"
                     /translation="MPARSPQSLVLAGVAALLLAMTSTEGTLTMLPGLLLMTAGGLWE
                     ALRSRAEAAAAPPLARARPAARRRRTGVNFGRGELRQSRT"
     gene            complement(3612..4793)
                     /locus_tag="VNU01_04390"
     CDS             complement(3612..4793)
                     /locus_tag="VNU01_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH31888.1"
                     /translation="MSRGKKLLALALPPGQAFVEAWRRAWDEGHAVLPVDPRLPAAQL
                     RRLLDRLRPHELTDASGTRVLGGGAPLDPRTDLVVATSGSTGARKGAELSRAAVEWSA
                     AASLARLDAAGEPWLCCLPLDHLAGLQVVVRARLSGAPLLLHERFDVGRVAAEGPGAF
                     VSLVPTMLTRLLDAGADVAAFRGILLGGAAADPALLRRAREAGARVVTTYGMTETAGG
                     CVYDGVPLDGVDARVGQDGRIGLRGPMLFHVYRLAPGLSAAALAGGWFTTADRGAWRD
                     GRLVVLGRADDVVVTGGHNVSLDEVADLVATHPAVAEAAAFALPDSEWGALVAVAVVP
                     REPAAPPTLAQVRAHVRARAAAHAAPKRLLIVTALPRGALGKVDREALRALAASDDAA
                     R"
     gene            4814..5683
                     /locus_tag="VNU01_04395"
     CDS             4814..5683
                     /locus_tag="VNU01_04395"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HWH31889.1"
                     /translation="MSRASVYWEAARPRTLPAAVAPVLVGTAAAERFLPGRLLLALVV
                     GLALQVGVNYANDYFDGVRGVDTPERVGPRRAVASGLVAPAAMRAAMLVAFGLAGAAG
                     LALALLVEPWLLAVGAVCLAAAAGYSGGPRPYASAGMGELFVFVFFGLVATVGSAYAQ
                     DERVVGAAVAGAVPMGLLASAILVVNNLRDITTDARAGKRTLAVRLGRGRTRLLFAAL
                     VAGAFAAVPAIAALDGAVAPLLALAAAPLAVPPLRIVWTREEPRPLIAALGGTARLEL
                     AVGVLLAAGLWLS"
     gene            5695..>6600
                     /locus_tag="VNU01_04400"
     CDS             5695..>6600
                     /locus_tag="VNU01_04400"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:NF002782.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HWH31890.1"
                     /translation="MTPVPFRIPMRTRFRGVTERAGVLLRGPAGWGEFSPFPEYGAEV
                     AARWLGAALEAAERGWPPPLRDAVPVNATVPAVGPEQAAAIVRAGGCATAKVKVAEPG
                     QSANDDLERVEAVRDALGPHGRIRVDANGAWDVDTAVRGLRALARFDLEYAEQPVATL
                     EEMAVLRRRVDVPLAADESVRTAEDPLRVAGLEAADIVVLKVQPLGGVRRALEVAEAC
                     GLPVVVSSAVETSVGLAAGVALAAALPELPYACGLGTQWMLEGDVVVDPLAPAGGMLE
                     VRVPRVSEELLARWVPGQGESRALLR"
BASE COUNT          729 a         2496 c         2550 g          825 t
ORIGIN      
        1 ggagggcatc ccgtacagcc gctcgccgga gccgcgcaac ccgcggctca tcgccgagct
       61 ctacgtcggc gagctggggc tcgaccggcc catccccgcc tcgcagttcg accgcgacgg
      121 cggcgaggtg atcggcgggc cggcggtgct cgcgcccggc ggcagcgccg agatggccgg
      181 cggcaacctg accctggagt tcggtgagct gcgcatgtgg agcgggttcc aggtcagcca
      241 cgcgcccggc cgcggactgc tcctggccgc ggcggtcctg ctgctggggg ggctggtacc
      301 ctcgctgtac agctaccggc gtcgcgtgtg ggtggaggcg caccccgacg gtgagggcac
      361 tcgcgtggtg ctcgccggcg tcgccctcca gcgcaaggcc accttcgccg acgaattccc
      421 gcggctcgcc gcgcgcgtgc gcgcggcggt gcccccgacc gacgaggcat gacgacgatg
      481 gtcgacgagc aactggcggc ggcgtccaac accctgtgga acgtggcctt cgtgggctac
      541 gtggtggcga tggtctgcta cttcttccgc ctcgcgttca ccaaggtcag caccgagggc
      601 gtcacggtca gcaccgcggc cggccgccgc gtcggcatgg ccgggaccgc gatcgccgtc
      661 gcggccgccg gcgtgcacac ggtcagcgtg gtgcagcgcg gcctggccac tggccgcgtg
      721 ccgtgggcga acatgtacga gtacagctcg ctcatggcgc tgctggcggt ggtggcgggg
      781 ctggtggtgg tgcagcggcg cttcggctac ggccacctca tgggcttcgt gctggcgctg
      841 gcggtgctgt ccatgacctc ggcgctgctg ctgtacgccg agccggggcc ggtcgtgccg
      901 gcactgcaga gctactggat caagatccac acctccgcgg ccatggcgtc gtcgtcgatc
      961 ttcatggtgg gcttcgcggc caccgcgctg tacctggtca aggacaccgc ggagcggcgc
     1021 gtcgccgcgc gcagcggcgc ctcgttcggg ccgggcagcg tcggggcggc ggaggccggc
     1081 gcgcagctcg ccgagggcta cgaggccgac accggcgtgc cggacgcgga ccgcgtcgcg
     1141 gacgccaccg cgcagcgcga cgtgctgtcg cccgtgctgt tcccggtgct gccgttcctg
     1201 gtgatcggcg tgttcaccct ggcggtgtgg caggcgcccg tggcggcgct gctggccggc
     1261 tccgtgggcg cgctgctggg cgcgtcgtcg tggtacgcgg tgccatatct gccggccgcc
     1321 gcgcgcctgg acaacctggc ctaccgggtg atcgcgttcg cgttccccat ctggacgttc
     1381 gcggtcatcg ccggggcgat ctgggccgag gaggcgtggg gccggtactg gggctgggac
     1441 cccaaggaga ccggcagctt cttcacgtgg gtgctgtacg ccagctacct gcacgcccgc
     1501 tcgacgcacg gctggcgggg ccgccgcgcc gcgtggatcg ccgtcgtggc gttcgtggcg
     1561 ctgatgatca cctactacgc cgtgaacctc tgggtggtcg gcctgcacag ctacgcgggg
     1621 gtctgaagac ccccggggta cgccggcgtc tgacaccgcc cggagaaagt gctcactgtg
     1681 agcggttact caggtgggcg tcggggtcgg cgtcgaggtc gacatctggg tcgggctggt
     1741 cgtcgagcag gtgcgcgggg atcggcgggg tgccgtcggc ctgcaccgag cccttgcgca
     1801 cgccctcggc gccgtcggtc gggcagcgct cctcccagcc catgagctcg cggcggcacg
     1861 ccgggcacac gcgcaggccc aggtccaccc acggcgagga gccgcccggc tcctcctcgt
     1921 caccctggtc gcgctcgcgg acgatcccca tcgttcactc ccaacgtctc atccctcgta
     1981 cagctcggcg ggcctggtca caccttcgcc gccgcgcgcc agcacgacaa gggactccgc
     2041 ctcgccgtac ggggacgcgg ccagggcggc gcgcaggcgc gcgacctccg cggcaccgtc
     2101 cacgcccgtc gcccagcgcg ccgtcgcggc gccggcggcg gtcgtgccgc gcagcgccaa
     2161 ggcgtccacc gcctcgccgt cgccggtggg ccaccacggc ccgcacgacg ccgcctccac
     2221 cccgccgagc cagtcgcgcg cgacggctgc gaacgccgcc tgctgcccgg ccgccagtgc
     2281 cgcacggttg gcctgcagga ccgcaccgcc gcggcccagc tcgaacgcct cccggtgggg
     2341 cagcaccagg cccagccaca gccgcagcag cgggtcgcgg acctggtagc ggacgcgctt
     2401 ggtgcggcgc gggtcctcgc ccacgggcgt cagccggtcc agcaggccca gtccctccag
     2461 cgccgccagt gcctcgcttg tggtggccgg cgcgaagccg gtgcgctcgc ggatcgcgcc
     2521 gaacgtgtcg gcgcccagcg cgaccagctc caggattcgg gcgggcgccg agcccgcggc
     2581 cagctcctgc agcaccactg tgccctcgtc gcccagcggc gcgccggggc cgtcgagcag
     2641 ctcggtgagg ttggcgaccg cgtcgtggcg cggatcccac agcgccaggt agcgcggcat
     2701 gccgccgacg gtgacgtagg cctccagcca ggccgccggg tccccgccgc ccacgagcag
     2761 gcccgcctcg cgccaggaga acggcgccag ccgcaggtgc gcgtcggcgc ggccgaacag
     2821 cggcgcgtcc gccgcgacca gctcctccat cagcccgacg tggctgcccg cgaggatcac
     2881 cgacaggcgc gagtcgccac ggtggtccca gcgcgcctgg agcgtcgagg gcagtgaagg
     2941 gtccgcctcg cacaggtacg ggaactcgtc cagcaccacc agcagcggct cctcggcccc
     3001 gcgcgcgatc aggtatccca gtgcggcgtc ccaggagtcg aagtgcccca ggtccagcag
     3061 gtcgccggcg gcggggcgca ccgccgcgcg cacgcgctcc tccaagcggc gcagcgcctc
     3121 ggcgaccggc gcgcgcgtgc ccggcaggaa gatggccggg gcgtcgcggc cagaggcgaa
     3181 gcggtccagc agcgccgtct tgccgacgcg ccggcgcccc gagaccacgg ccatgccgcc
     3241 cccagggccg cgttccggcc cacaggcccg cgacagggcg gtcagttcac gttcgcgatt
     3301 gacgaagttc gccacgaccg aagtttaccc cggtgcggcg tcgtcgcgcc gcaggccggg
     3361 cacgcgcgag gggaggggcc gcggccgcct cggcgcggga gcgcagggcc tcccacaacc
     3421 cgccggcggt catcagcagc agtccgggca gcatggtgag cgtgccctcg gtcgaggtca
     3481 tggccagcag cagcgcggcg acgcccgcca ggaccagcga ctgcggtgag cgggcgggca
     3541 cggcggcacc tttcgagacg ggtagtccgt tgtgcccgtt cccgcctcgg cgtacgcgcc
     3601 cggccgcggc gtcaccgggc cgcgtcgtcc gatgccgcca gtgcgcgcaa ggcctcgcga
     3661 tcgaccttgc ccagggcgcc gcgcggcagc gccgtgacga tcagcaggcg cttgggggct
     3721 gcgtgcgcgg cggcgcgggc gcgcacgtgc gcccgcacct gcgccaacgt cgggggcgcg
     3781 gcgggctcgc gcgggaccac cgcgaccgcg acgagggcgc cccactccga gtccggcagc
     3841 gcgaacgcgg cggcctcggc caccgccggg tgggtggcca ccaggtcggc cacctcgtcc
     3901 agcgacacgt tgtggccgcc ggtgaccacc acgtcgtcgg cgcggccgag caccaccagg
     3961 cgcccgtcgc gccacgcgcc ccggtcggcc gtggtgaacc atccgccggc cagcgccgcg
     4021 gcgctcaggc cgggagccag ccggtagacg tggaacagca tcggcccgcg caggccgatg
     4081 cgaccgtcct ggcccacgcg ggcgtccacg ccgtccagcg ggacgccgtc gtagacgcag
     4141 ccgcccgcgg tctcggtcat gccgtaggtg gtcaccacgc gcgcgccggc ctcgcgcgcg
     4201 cgccgcagca gcgccgggtc ggcggccgcg ccgcccagca ggatcccgcg gaaggccgcg
     4261 acgtccgccc ctgcgtccag cagccgcgtc agcatcgtcg gcaccaggga gacgaacgcc
     4321 ccgggcccct ccgcggcgac gcggccgacg tcgaagcgct cgtgcagcag cagtggcgcc
     4381 ccggacagcc gtgcgcgcac caccacctgg agcccggcca ggtggtccag cggcaggcag
     4441 cacagccacg gctccccggc ggcgtccagc cgcgccagcg acgccgccgc tgaccactcg
     4501 accgccgcgc gagacagctc ggcgcccttg cgcgcccccg tggagcccga ggtggcgacc
     4561 accaggtccg tgcgcgggtc cagcggagcg ccgccgccca gcacgcgcgt gccggaggcg
     4621 tcggtcagct cgtgcggtcg cagccggtcg agcagccgtc ggagctgcgc cgccggcagc
     4681 cgcgggtcca cgggcagcac ggcgtggccc tcgtcccacg cgcgccgcca cgcctccacg
     4741 aaggcctgcc cggggggcag cgccagcgcc aggagtttct tgccgcggct catgagtcgc
     4801 tacggtaggc gacgtgagcc gcgcatcggt ctactgggag gcggcgcgcc cccgcacgct
     4861 gccggccgcc gtggccccgg tcctggtcgg cactgccgcc gccgagcgct tcctgccggg
     4921 gcgcctcctg ctcgcgctgg tggtcggcct ggcgctgcag gtcggcgtca actacgccaa
     4981 cgactacttc gacggcgtcc gcggcgtgga cacgccggag cgggtgggcc cgcggcgcgc
     5041 cgtcgccagc ggcctggtgg cgcccgccgc gatgcgggcg gccatgctcg tggcgttcgg
     5101 gctcgccggc gccgccgggc tggccctcgc gctgctggtc gagccgtggc tcctggcggt
     5161 cggcgcggtg tgcctggcgg ccgccgccgg ctacagcggc ggcccccggc cgtacgcctc
     5221 ggccggcatg ggcgagctgt tcgtgttcgt cttcttcggg ctggtggcca cggtcggctc
     5281 cgcctacgcc caggacgagc gcgtcgtcgg cgcggccgtg gccggcgcgg tgcccatggg
     5341 cctgctggcc agcgcgatcc tggtggtgaa caacctgcgc gacatcacga cggacgcccg
     5401 ggcgggcaag cgcacgctgg ccgtgcgcct gggccggggc cgcacgcggc tgctgttcgc
     5461 cgcgctggtg gccggtgcgt tcgccgccgt gcccgccatc gcggcgctcg acggcgccgt
     5521 ggcgccgctg ctggcgctgg ccgcggcacc gctggccgtc ccgccgctgc ggatcgtgtg
     5581 gacccgggag gagccgcggc cgctcatcgc cgcgctgggc ggcacggccc ggctggaact
     5641 ggccgtcggc gtgctgctgg ccgcggggct gtggttgtca tgactggggt ggccatgacg
     5701 ccggtgccgt ttcgcatccc catgcgcacg cgcttccgcg gcgtgacgga gcgcgccggc
     5761 gtgctgctgc gcggaccggc cgggtggggc gagttctcgc cgttccccga gtacggggcc
     5821 gaggtggcgg cgcgctggct cggtgccgcg ctggaggcgg ccgaacgggg ctggccgccg
     5881 ccgctgcgcg acgccgtgcc ggtcaacgcg acagtgcccg ccgtgggtcc cgagcaggcc
     5941 gcggccatcg tccgcgccgg tggctgcgcc acggcgaagg tgaaggtggc cgagccgggc
     6001 cagtcggcga acgacgacct ggagcgcgtc gaggcggtgc gcgacgcgct gggcccgcat
     6061 ggccgcatcc gcgtcgacgc caacggcgcg tgggacgtcg acaccgccgt gcgcggcctg
     6121 cgcgcgctgg cgcgcttcga cctggagtac gccgagcagc cggtcgcgac cctggaggag
     6181 atggccgtgc tgcgccggcg cgtcgacgtc ccgctggcgg ccgacgagtc cgtgcgcacg
     6241 gcggaggatc cgctgcgcgt cgccgggctg gaggccgccg acatcgtggt gctgaaggtg
     6301 cagccgctgg gcggggtgcg ccgggcgctg gaggtggccg aggcgtgcgg cctgcccgtg
     6361 gtggtgtcca gcgcggtgga gacctccgtc ggcctggccg cgggggtggc gctggccgcc
     6421 gcgctgccgg agctgcccta cgcctgcggc ctcggcacgc aatggatgct ggagggcgac
     6481 gtggtggtgg acccgctcgc gccggccggc ggcatgctgg aggtccgcgt gccgcgggtc
     6541 tccgaggagc tgctggcacg ctgggtgccc gggcagggtg agtcgcgggc gctgctgcgc
//
LOCUS       DATMKC010000147         5874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_52000, whole genome shotgun sequence.
ACCESSION   DATMKC010000147 DATMKC010000000
VERSION     DATMKC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52000"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VNU01_04405"
     CDS             complement(<1..813)
                     /locus_tag="VNU01_04405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWH31891.1"
                     /translation="MLIVQRPTITENVIAQDLRSTFTIEPLEPGFGYTLGNSLRRTLL
                     SSIPGAAVTSVRIEGVLHEFSSVEGVKEDVTDIILNLKELVLRCHSDEPVEIFLGGEG
                     PGEITADFITAPSEVEILNRDLHIATLNRRGRVEMYLTVERGRGYVSAERNKRADQPI
                     GVIPIDSIYSPVRRVTYRVEPTRVEQMTNYDRLILDVESNGAVTPGEALSSAGETLRD
                     LLGLFAEFEESGPGGLAIGADTSASAPSSPNLLLPIEDMDLSVRSYNCLKREG"
     gene            complement(859..1485)
                     /gene="rpsD"
                     /locus_tag="VNU01_04410"
     CDS             complement(859..1485)
                     /gene="rpsD"
                     /locus_tag="VNU01_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWH31892.1"
                     /translation="MARYTGPDCKLCRRERQKLYLKGTRCEGPKCAIEKRPYPPGEHG
                     RGRIKESEYLLQLREKQKARRIYGVLEKQFRNYYQEASRQQGLTGENLLVLLESRLDN
                     VVYRGGLARSRDEARQLVRHRHFQVNGRTVNIPSYRVRPGEVVTVRERARNIVPILGA
                     LELFGMRQIPGWLSTDRTKLQISVLDRPSRSQIDVPVQEQLIVELYSK"
     gene            complement(1544..1957)
                     /gene="rpsK"
                     /locus_tag="VNU01_04415"
     CDS             complement(1544..1957)
                     /gene="rpsK"
                     /locus_tag="VNU01_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWH31893.1"
                     /translation="MAQQKKQAGAKAKSRARKKERRQVAYGQAHIKSSFNNTIVTITD
                     QQGNVLSWATAGNVGFKGSRKSTPFAAQLAAEKAAKGAAEYGVRKVDVFVKGPGSGRE
                     TAIRSLQANGLEVAVIKDVTPIPHNGCRPPKRRRV"
     gene            complement(2012..2392)
                     /gene="rpsM"
                     /locus_tag="VNU01_04420"
     CDS             complement(2012..2392)
                     /gene="rpsM"
                     /locus_tag="VNU01_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWH31894.1"
                     /translation="MARIAGVDLPREKRVEIGLTYIFGVGRTRSQQTLAACGISRDTR
                     VRDLSDDEVRRLRDFIDASFKVEGDLRREVAQNIKRKIEINSYQGIRHRRGLPVRGQR
                     THTNARTRKGPKRTVGGLKKKLRK"
     gene            complement(2439..2552)
                     /gene="rpmJ"
                     /locus_tag="VNU01_04425"
     CDS             complement(2439..2552)
                     /gene="rpmJ"
                     /locus_tag="VNU01_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWH31895.1"
                     /translation="MKVQPSVKKICEKCRVIRRHGRIMVICSNPRHKQRQG"
     gene            complement(2695..3549)
                     /gene="map"
                     /locus_tag="VNU01_04430"
     CDS             complement(2695..3549)
                     /gene="map"
                     /locus_tag="VNU01_04430"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWH31896.1"
                     /translation="MIVRKSPSEIALMAKAGRVVAQVHEILREALRPGMTTADLDAIA
                     EREIVSRGAVPSFKGYRGFPATLCTSVNAQIVHGIPSPDVVLREGDLVKIDAGAIVDG
                     WHGDSAVTWVVGAEDGAADPALVGPEVAELVERTRAGMWAGLCQARDGRRLTDISAAV
                     EAFALPKGYGVVREYVGHGIGRALHEDPHVPNYGRAGRGPKLVAGLVLAVEPMFNLGS
                     EETETLEDDWTVVTADGSLSAHWEHTVAITPEGPWVLTARSDEPEWPLAQPELVPAFR
                     GQVDGVRG"
     gene            complement(3546..4232)
                     /locus_tag="VNU01_04435"
     CDS             complement(3546..4232)
                     /locus_tag="VNU01_04435"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWH31897.1"
                     /translation="MRLVLLGPPGAGKGTQATEIGRNYDVPLISTGDIFRANVKQGTP
                     LGQEAKSFMDRGALVPDDIVIRMVEDRLGQDDCADGFVLDGFPRTVPQAEALDEFLVA
                     RDCPLDVVLRFDVPVEEVVNRVVNRRVCPNCGAVYHLHYAPPAVDGVCDNCGAGLIQR
                     DDDTEEVVRARMDEYRAKTEKLERYYAERGILRDVKAVGAVEDVTRRTLEALEEFQKP
                     DDPGRGGGSS"
     gene            complement(4243..5535)
                     /gene="secY"
                     /locus_tag="VNU01_04440"
     CDS             complement(4243..5535)
                     /gene="secY"
                     /locus_tag="VNU01_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWH31898.1"
                     /translation="MIRAFANAFKIPDLRGKILFTLAILAVYRLGSFIPIPGIDYGAL
                     QDVLEQSQAAGAAALLNLFSGGALSQLAVFALGIMPYITASIIMQLLTVVIPKLEEWQ
                     KEGESGTKRITQWTRYLTIVLAVLQSTGMIVLIQSGQLFGNQIDTTGLIPNPTIGTRA
                     LMVLTLTAGTAFIMWLGELITQRGIGNGMSILIFASIISQLPAQGSQILQTAGAVVFV
                     IVMFTGLLLLVGVVFMEQGQRRIPVQYAKRQLGRRTYGGTQTYIPLKVNQSGVIPIIF
                     ASSLLYLPQLAASIVQNERLNTFVQRYFTSQDHPVYILLFAAMVIFFAYFYTAITFNP
                     VQVSDNMKKYGGFIPGIRPGRPTAEYLDRVLTRITLPGSIYLAGLAVLPMIVLGVANV
                     QFPFGGATLLIVVGVGLETMKQIESQLMQRHYEGFLGG"
     gene            complement(5569..>5874)
                     /locus_tag="VNU01_04445"
     CDS             complement(5569..>5874)
                     /locus_tag="VNU01_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HWH31899.1"
                     /translation="LQRRLPKLPGFTPRNRVAYVGVNVAKLDALFSDGDAVTPEALRE
                     RGLIRKLSEPVKILGNGDLGKKLTVSAHAFSESARQKIDAAGGTVSVLPRKQAADSQ"
BASE COUNT          838 a         2022 c         2034 g          980 t
ORIGIN      
        1 gccctcgcgc ttgaggcagt tgtaggaccg caccgacagg tccatgtcct cgatcggcag
       61 cagcaggttc ggggagctgg gtgccgacgc ggacgtgtcc gcgccgatcg ccaggccgcc
      121 gggaccggac tcctcgaact cggcgaacag ccccagcagg tcgcgcagcg tctcgccggc
      181 gctggacagc gcctcgcccg gcgtgacggc gccgttggac tccacgtcca ggatcagccg
      241 gtcgtagttg gtcatctgct ccacacgggt gggctccacc cggtaggtga cgcggcgcac
      301 cggcgagtag atggagtcga tcgggatgac accgatcggc tggtcggcgc gcttgttgcg
      361 ctccgccgag acgtagccgc ggccacgctc cacggtgagg tacatctcca cgcgcccgcg
      421 gcggttcagc gtggcgatgt gcaggtcgcg gttgaggatc tcgacctcgc tgggcgccgt
      481 gatgaagtcc gcggtgatct caccggggcc ctcaccgccc aggaagatct cgaccggctc
      541 gtcgctgtgg cagcgcagga ccagttcctt gaggttcagg atgatgtcgg tgacatcctc
      601 cttgacgccc tcgacggagc tgaactcgtg cagcacgccc tcgatgcgga cgctggtcac
      661 cgccgcgccg gggatcgacg acagcagggt ccggcgcagg ctgttgccga gcgtgtagcc
      721 gaagcccggc tccaggggct cgatggtgaa cgtcgaccgc aggtcctgcg cgatgacgtt
      781 ttcggtgatg gtggggcgct ggacgatcag cacgtttgtc tcgcctccgc tggtgggcgt
      841 tcgtcggggt ccgctgcgct acttggagta gagctcgacg atgagctgct cctgcacggg
      901 cacgtcgatc tgcgaccggc tgggccggtc cagcaccgag atctgcagct tggtgcggtc
      961 cgtggacagc cagcccggga tctggcgcat gccgaacagc tccagcgcgc ccaggatcgg
     1021 cacgatgttg cgggcgcgct cacggaccgt cacgacctcg ccgggacgga cccggtagga
     1081 cgggatgttc acggtgcggc cgttgacctg gaagtggcgg tggcgcacca gctggcgggc
     1141 ctcgtcgcgc gagcgggcca ggccgccgcg gtagacgacg ttgtccaggc gggactccag
     1201 cagcaccagc aggttctcac cggtcaggcc ctgctggcgc gaggcctcct ggtagtagtt
     1261 gcggaactgc ttctccagca cgccgtagat gcggcgggcc ttctgcttct cccgcagctg
     1321 gagcaggtac tcgctctcct tgatgcggcc gcggccgtgc tcacccggcg ggtacgggcg
     1381 cttctcgatg gcgcacttgg ggccctcgca gcgcgtgccc ttgaggtaga gcttctggcg
     1441 ctcacggcgg cagagcttgc aatccggtcc ggtgtaccga gccatggtgt gttctcttcc
     1501 ctcttcgaga tcttgtggtc agacccggcg cctcagcgtc aacctaaacg cgacggcgct
     1561 tgggcggccg gcagccgttg tgcgggatgg gcgtgacgtc cttgatgacc gcgacctcca
     1621 ggccgttggc ctgcagcgac cggatggcgg tctcgcggcc ggagcccggg cccttcacga
     1681 acacgtcgac cttgcgcacg ccgtactcgg ccgcgccctt ggccgccttc tccgcggcga
     1741 gctgggcggc gaagggcgtc gacttgcggc tgcccttgaa gcccacgttg ccggcggtcg
     1801 cccacgacag gacgttgccc tgctggtcgg tgatcgtgac gatggtgttg ttgaaggagc
     1861 tcttgatgtg cgcctgcccg taggcgacct ggcgccgctc cttcttgcgc gccctggact
     1921 tcgccttggc gcccgcctgc ttcttctgct gagccatcga gtgggtcctt cgtgttcgtg
     1981 gaggtgatcg cggtgggccg gatcggcccg gctacttccg cagcttcttc ttcagaccgc
     2041 cgaccgtgcg cttgggcccc ttgcgggtgc gcgcattggt gtgcgtgcgc tggccgcgga
     2101 ccggcaggcc acggcggtgg cggatgccct ggtaggagtt gatctcgatc ttgcgcttga
     2161 tgttctgcgc cacctcgcgg cgcaggtcgc cctccacctt gaacgaggcg tcgatgaagt
     2221 cgcgcagacg gcggacctcg tcgtcggaca ggtcgcgcac ccgggtgtcc cggctgatcc
     2281 cgcatgccgc cagcgtctgc tgcgagcggg tccggccgac gccgaagatg taggtcaggc
     2341 cgatctcaac ccgcttctcg cgggggaggt cgacaccggc gatacgtgcc atgcgtgcgt
     2401 gctcctgtgt ctgtccgagg tggtcgcgac gggcggtgct agccctgccg ctgcttgtgc
     2461 cgcgggttgc tgcaaatgac catgatccgg ccgtgccggc ggatgacgcg gcacttctca
     2521 cagatcttct tcaccgaggg ctggaccttc atggtgtgcg agccttcttg ttcgcggcgc
     2581 gaggcaagcc ccgctcgagc cgatggatgt cgtccccgcc ggtcgcgcac ggcgcaccag
     2641 ggccggaaca ggacaaagac cccgccgtgg cggagccgaa cgccaacctt agcatcagcc
     2701 cctcaccccg tccacttggc cgcggaacgc gggcaccagt tcgggctggg ccagcggcca
     2761 ctccggctcg tccgagcggg cggtgaggac ccaggggccc tccggggtga tcgcgacggt
     2821 gtgctcccag tgcgccgaca ggctgccgtc ggcggtcacg acggtccagt cgtcctcgag
     2881 cgtctccgtc tcctcagagc ccaggttgaa catcggctcc accgcgagca ccaggcccgc
     2941 gaccagcttg ggaccgcggc cggcgcggcc gtagttgggc acgtgcgggt cctcgtgcag
     3001 cgccctgccg atgccgtggc cgacgtactc gcgcaccacg ccgtagccct tgggcagcgc
     3061 gaacgcctcc accgccgcgg agatgtccgt gagacgcctg ccgtcccggg cctggcacag
     3121 gccggcccac atgcccgcgc gggtgcgctc gaccagctcg gcgacctcgg gacccaccag
     3181 cgcaggatcg gccgcgccgt cctcggcgcc gaccacccag gtcaccgcgc tgtcgccgtg
     3241 ccagccgtcc acgatggcgc ccgcgtcgat cttcaccagg tcgccctcgc gcaggaccac
     3301 gtccggcgac gggatgccgt gcacgatctg ggcgttgacg gacgtgcaca gcgtggcggg
     3361 gaagccccgg tagcccttga acgacgggac ggcgccgcgt gagacgatct cccgttcggc
     3421 gatcgcgtcc aggtccgcgg tggtcatgcc cggccgcagc gcctcgcgca ggatctcgtg
     3481 cacctgggcg acgacccggc cggccttcgc catcagggcg atctcgctgg gcgacttgcg
     3541 gacgatcacg acgaaccgcc gccccgcccg ggatcgtccg gcttctggaa ctcctccagc
     3601 gcctccagcg tgcggcgcgt cacgtcctcg acggcgccga ccgccttcac gtcgcgcagg
     3661 atgccccgct cggcgtagta gcgctccagc ttctccgtct tggcgcggta ctcgtccatg
     3721 cgcgcgcgga ccacctcttc ggtgtcgtcg tcgcgctgga tcagccctgc gccgcagttg
     3781 tcgcagacgc catccaccgc cggcggcgcg tagtgcaggt ggtacaccgc gccgcagttc
     3841 gggcacacgc gccggttcac cacccggttg acgacctcct ccaccggcac gtcgaagcgc
     3901 agcaccacgt ccagcgggca gtcgcgggcc accaggaact cgtccagtgc ctcggcctgc
     3961 ggcacggtgc gcgggaagcc gtccaggacg aagccgtccg cgcagtcgtc ctggcccagc
     4021 cggtcctcga ccatgcggat gacgatgtcg tcgggcacca gcgcgccacg gtccatgaag
     4081 gacttcgcct cctggcccag cggcgtgccc tgcttgacgt tggcgcggaa gatgtcgccc
     4141 gtggagatca acggcacgtc gtagttccga ccgatctcgg tcgcctgggt tcccttgccg
     4201 gcgcccggag ggccgaggag cacgagtcgc acgatgcctc tcctagcccc cgaggaagcc
     4261 ctcgtagtgg cgctgcatca gctggctttc gatctgcttc atggtctcca ggcccacgcc
     4321 caccacgatc agcagcgtgg cgccgccgaa cgggaactgc acgttggcca cgcccagcac
     4381 gatcatcggc aggacggcca gccccgccag gtagatggag ccgggcaggg tgatgcgggt
     4441 cagcaccctg tccaggtact ccgccgtggg gcggcccggg cggatgcccg ggatgaaacc
     4501 gccgtacttc ttcatgttgt ccgagacctg gacggggttg aacgtgatcg ccgtgtagaa
     4561 gtaggcgaag aagatcacca tcgccgcgaa cagcaggatg tacacggggt ggtcctggct
     4621 cgtgaagtag cgctggacga aggtgttgag gcgctcgttc tgcacgatcg acgcggccag
     4681 ctgcggcagg tacagcagcg acgacgcgaa gatgatcggg atcacgccgg actggttgac
     4741 cttcagcggg atgtaggtct gcgtgccgcc gtaggtgcgt cggccgagct ggcgcttggc
     4801 gtactgcacc gggatgcgcc gctggccctg ctccatgaac accacgccca ccagcagcag
     4861 cagcccggtg aacatcacga tgacgaacac cacggcgccg gcggtctgca ggatctggct
     4921 gccctgtgcc ggcagctggc tgatgatcga ggcgaagatc aggatcgaca tgccgttgcc
     4981 gatgccgcgc tgcgtgatga gctcgcccag ccacatgatg aacgcggtgc cggcggtgag
     5041 cgtgagcacc atcagggcgc gcgtgccgat ggtcgggttc gggatcagcc ccgtggtgtc
     5101 gatctggttg ccgaacagct gcccggactg gatcagcacg atcatgccgg tggactgcag
     5161 gacggccagg acgatggtca ggtagcgcgt ccactgggtg atgcgcttgg tgccgctctc
     5221 accctccttc tgccactcct ccagcttcgg gatcaccacc gtgagcagct gcatgatgat
     5281 gctcgcggtg atgtacggca tgatccccag cgcgaacacc gccagctggc tcagcgcgcc
     5341 accggagaac aggttcagca gcgcggcggc gcccgcggcc tgcgactgct ccagcacgtc
     5401 ctgcagcgcc ccgtagtcga tgcccgggat ggggatgaac gaccccagcc ggtacaccgc
     5461 gaggatcgcg agcgtgaaca agatcttccc gcgcaggtcg gggatcttga aggcgtttgc
     5521 gaaagctcgg atcatcgcga tgactcctgg tcgaggggcc ccccgcccct actggctgtc
     5581 cgcggcctgc ttgcgcggca gcacgctgac cgtaccgccc gccgcgtcga tcttctgccg
     5641 cgccgactcg gagaaggcgt gggcggagac cgtgagcttc ttgcccaggt cgccgttgcc
     5701 aaggatcttc accggctcgg acagcttgcg gatcaggccg cgttcgcgca acgcctccgg
     5761 cgtgacggcg tcaccgtcgc tgaacagcgc gtccagcttc gcgacgttca cgccgacgta
     5821 ggcgacgcgg ttgcgcggcg tgaagccggg cagcttgggc aggcgccgct gcag
//
LOCUS       DATMKC010000148         4150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_99702, whole genome shotgun sequence.
ACCESSION   DATMKC010000148 DATMKC010000000
VERSION     DATMKC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99702"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..250
                     /locus_tag="VNU01_04450"
                     /pseudo
     CDS             80..250
                     /locus_tag="VNU01_04450"
                     /inference="COORDINATES: protein motif:HMM:NF020025.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; incomplete; partial
                     in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
     gene            320..739
                     /locus_tag="VNU01_04455"
     CDS             320..739
                     /locus_tag="VNU01_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31900.1"
                     /translation="MVEFVDGATVAKLSPPDMRLPIQLALAWPDRLDWAPTRMDWTSA
                     QALAFEPVDHDTFPMVALAMAAGRAGGTAPGVLNAANEEAVAAFLDRRLPFLGIAEVV
                     ADVLGSHEPVQASDVDTVLEAERWAREAARRLIDERD"
     gene            742..1878
                     /locus_tag="VNU01_04460"
     CDS             742..1878
                     /locus_tag="VNU01_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWH31901.1"
                     /translation="MYAAVFVVSLVLAIMFHEFGHYATAKAFGMKVERFFLGFGPTLW
                     SFQRGETEYGVKAIPAGGFVKITGHSRFEDIAEQDRPRAFYNQPAWQRFIVLAAGSFT
                     HFIVAVLLLFAALAFIGAPVASNLIEQVAPGTPADEAGLREGDRIVAADGVPVGDFEA
                     VRDIVTARGGERMTLVVERDGERLQVQARIAEQTPDGQEVGFLGVAPAAETRRYGVAA
                     AAREVTTGDLSIATLTRITVGGLAQALSPAGLAHYFSQVGSEGPRDPEGPISVVGIGQ
                     TVQALGSSGDVFAVLAILAQLNIVLGVLNMLPLPPLDGGHVMVMLVEKGVNGWRRLRG
                     RRADWSLDPAVVTPIALAVILLFTMLTVTAVYLDIVKPASELVQ"
     gene            1896..3056
                     /gene="ispG"
                     /locus_tag="VNU01_04465"
                     /gene_synonym="gcpE"
     CDS             1896..3056
                     /gene="ispG"
                     /locus_tag="VNU01_04465"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWH31902.1"
                     /translation="MTTSLPLLGSQPATSPAVEPPRRRPSRRINVGKIPVGDGAPVSV
                     QTMTTTPTHDVNATLQQIAAMNAAGVDIVRVAVPRQEDADALAQIAAKSTVPVIADIH
                     FQWKYAMAAIEAGVAGVRINPGNIRKHDKVKLIGDAARERGIPIRIGVNGGSLEDVIL
                     AKHGGVTPEAMVESALNEARLLEEVDFFDIKISVKHSDPWVMIQTYRLLAERCDYPLH
                     LGVTEAGPLKTGTIKSAVGIGALLAEGIGDTIRVSLSADPVEEVKAGIAILESLHLRE
                     RGLDVVSCPSCARAEVDVYDLAERVQAALEGIDVPLRVAVMGCVVNGPGEAREADVGV
                     AAGKGKGQLLYKGQVVGQVPEDEIVERLVELAREVAEEIRAERGSGPPVVTA"
     gene            complement(3113..3859)
                     /locus_tag="VNU01_04470"
     CDS             complement(3113..3859)
                     /locus_tag="VNU01_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HWH31903.1"
                     /translation="MARTTRTSESYGAAAAAPSLIRWGAVFGGTVVGLSLLMLLTTLL
                     FALAFGSGMETIEANLPWYIAGAAVLCMFIGGMVAGWLSGVPGAGPGFFNGLSVWALI
                     LLAALIVGVPSLVGAAGIDISLADTPNTDQIAAQGDGLWAGFFSLLAGALTAAIGGMI
                     GGVITRPAFAPMSETVITDHGRPMPVGASDRAYDPRDERAYDERAMREEDHRPRDTGD
                     DRPRAERGTDDRPRAGADRRSDGNGWTTST"
     gene            3953..>4150
                     /locus_tag="VNU01_04475"
     CDS             3953..>4150
                     /locus_tag="VNU01_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWH31904.1"
                     /translation="MTPDEARSFLAERHAGVLATLRRDGRPQLSNVVYALIDGRVRVS
                     VTDTRAKVANLRRDPRVSLHVT"
BASE COUNT          570 a         1481 c         1532 g          567 t
ORIGIN      
        1 gtggagccga ggatggtgac gctcgtggtc atgaccgcga cagccgggcc aggcgctcgg
       61 cggccgcgcc ggcgaggtcg cccggctggt gctcaccgcc agcggcgggc cgttccgcgg
      121 gcgcacccgc gccgagctcg cgggcgtcac gcccgagcag gcactggcgc accccacgtg
      181 ggacatgggc gcgctggtca cgatcaactc ggccaccctg gccaacaagg gcctggaggt
      241 catcgaggcg cacgtgctgt tcgggctggc ctacgagcgc atcgacgtgg tggtgcaccc
      301 gcagagcgtc gtgcacggca tggtcgagtt cgtcgacggc gccaccgtcg ccaagctgtc
      361 cccgccggac atgcgcctgc ccatccagct cgcgctcgcc tggccggacc ggctggactg
      421 ggcgccgacg cgcatggact ggaccagcgc gcaggcgctg gcgttcgagc ccgtcgacca
      481 cgacaccttc cctatggtgg cgctggcgat ggcggcaggc cgcgcgggcg gcaccgcgcc
      541 cggcgtgctc aacgccgcga acgaggaggc ggtcgcggcg ttcctggacc ggcgcctgcc
      601 gttcctgggc atcgccgagg tggtcgcgga cgtcctggga tcccacgaac ccgtccaggc
      661 gtccgatgtg gacaccgtgc tggaggcgga acgctgggcg cgtgaggctg cgcgccgact
      721 gatcgacgag agggactagc gatgtacgcc gccgtgttcg tcgtgagcct ggtgctcgcg
      781 atcatgttcc acgagttcgg ccactacgcc acggccaagg cgttcggcat gaaggtcgag
      841 cgcttcttcc tgggcttcgg cccgaccttg tggtcgttcc agcgcggcga gaccgagtac
      901 ggcgtgaagg cgatccccgc cggcggcttc gtcaagatca ccggccactc gcgcttcgag
      961 gacatcgccg agcaggaccg gccgcgcgcc ttctacaacc agccggcctg gcagcgcttc
     1021 atcgtgctgg ccgccggctc cttcacgcac ttcatcgtcg ccgtgctgct gctgttcgcc
     1081 gccctggcct tcatcggcgc ccccgtggcc agcaacctga tcgagcaggt ggcgcccggc
     1141 acgcccgccg acgaggccgg cctgcgtgag ggcgaccgca tcgtcgccgc ggacggcgtg
     1201 cccgtgggcg acttcgaggc ggtccgcgac atcgtcaccg cgcgcggcgg cgagcgcatg
     1261 acgctggtgg tggaacgcga cggcgagcgg ctccaggtgc aggcgcgcat cgccgagcag
     1321 accccggacg gccaggaggt cggcttcctg ggggtcgcgc cggccgccga gacgcgccgc
     1381 tacggcgtgg ccgccgccgc gcgcgaggtc accaccggcg acctgtcgat cgcgacgctc
     1441 accaggatca ccgtgggcgg cctggcgcag gcgctcagcc ccgcgggcct cgcccactac
     1501 ttctcccagg tgggcagcga ggggccgcgc gaccccgagg gccccatcag cgtggtgggc
     1561 atcggccaga ccgtgcaggc cctgggctcc tccggcgacg tgttcgcggt cctggcgatc
     1621 ctggcgcagc tcaacatcgt gctgggcgtg ctgaacatgc tgccgctgcc gccgctggac
     1681 ggcggccacg tcatggtgat gctggtggag aagggcgtga acggctggcg ccgcctgcgc
     1741 ggccgccgcg ccgactggag cctggacccg gcggtggtga cgccgatcgc gctggcggtg
     1801 atcctgctgt tcacgatgct caccgtcacg gccgtctacc tggacatcgt caagcccgct
     1861 tcggagctgg tgcagtaggg cgatagcctg ggggcgtgac cacttccctt cccctgctgg
     1921 gctcgcagcc cgcgacgtcc ccggcggtcg agccgccgag gcgccgcccg tcgcggcgca
     1981 tcaacgtcgg caagatcccc gtcggcgacg gcgcgcccgt cagcgtccag acgatgacca
     2041 cgacgccgac gcacgacgtc aacgcgacgc tgcagcagat cgcggccatg aacgccgcgg
     2101 gcgtcgacat cgtgcgcgtg gccgtgccgc gccaggagga cgccgacgcg ctcgcgcaga
     2161 tcgcggcgaa gtccaccgtc cccgtcatcg ccgacatcca cttccagtgg aagtacgcga
     2221 tggccgcgat cgaggcgggc gtcgccggcg tgcgcatcaa ccccggcaac atccgcaagc
     2281 acgacaaggt caagctgatc ggcgacgccg cgcgcgagcg cggcatcccc atccgcatcg
     2341 gcgtcaacgg cggcagcctc gaggacgtga tcctggccaa gcacggcggc gtgacgccgg
     2401 aggcgatggt cgagtcggcg ctcaacgagg cgcggctgct ggaagaggtc gacttcttcg
     2461 acatcaagat ctcggtgaag cactcggacc cctgggtgat gatccagacc taccgcctgc
     2521 tggccgagcg ctgcgactac ccgctgcacc tgggcgtcac cgaggcgggc cccctgaaga
     2581 ccggcaccat caagtccgcc gtcggcatcg gggcgctgct cgccgagggc atcggcgaca
     2641 cgatccgcgt ctccctgtcg gccgaccccg tcgaggaggt caaggcgggc atcgcgatcc
     2701 tggagtcgct gcacctgcgc gagcgcggcc tggacgtggt gagctgcccg agctgcgcgc
     2761 gcgccgaggt cgacgtctac gacctggccg agcgggtgca ggccgccttg gagggcatcg
     2821 acgtgccgct gcgcgtggcc gtgatgggct gcgtggtgaa cggtcccggc gaggcgcgcg
     2881 aggccgacgt gggcgtggcc gccggcaagg gcaagggcca gctcctgtac aaggggcagg
     2941 tcgtcggcca ggtcccggag gacgagatcg tcgaacgcct cgtggagctg gcccgcgagg
     3001 tggccgagga gatccgcgcg gagcgcggct ccgggcctcc ggtggtcacg gcctgacctt
     3061 ccgggaccac ttgagtccga tcttggacac aagtggtccc gcagtggcga ggtcaggtgc
     3121 tggtggtcca gccgttgccg tccgagcggc ggtcggcgcc ggcgcgcggg cggtcgtcgg
     3181 tcccacgctc ggcgcgcggg cggtcgtcgc cggtgtcgcg ggggcggtgg tcctcctcgc
     3241 gcatcgcgcg ctcgtcgtag gcgcgctcgt cacgcgggtc gtaggcgcgg tcgctggcgc
     3301 ccaccggcat cgggcggccg tggtcggtga tgaccgtctc gctcatcggc gcgaacgccg
     3361 ggcgggtgat caccccgccg atcatgccgc cgatcgcggc ggtcagcgcg cccgccagca
     3421 gcgagaagaa gccggcccac aggccgtcgc cctgcgcggc gatctggtcg gtgttcggcg
     3481 tgtcggcaag gctgatgtcg atgccggccg cgcccaccag gctcggcacg ccgacgatca
     3541 gggcggccag caggatcagc gcccagaccg acagcccgtt gaagaagccc gggccggcgc
     3601 cggggacgcc ggagagccag ccggcgacca tgccgccgat gaacatgcac agcacggcgg
     3661 cgccggcgat gtaccacggc aggttcgcct cgatcgtctc catcccgctg ccgaacgcca
     3721 gggcgaacag cagcgtggtc agcagcatca gcagtgacag gccgaccacg gtgccgccga
     3781 acaccgcgcc ccagcggatc agcgagggtg cggccgcggc ggccccgtag gactcgctcg
     3841 tccgtgtggt gcgggccatg aggtcctcct tgggtcgcgg gcagggctta gggtgtccat
     3901 gcccgcgctg acctgccgct atgccttggc ggcgaggaga ccaggagacg cgatgacccc
     3961 cgacgaggcc cgttcgttcc tcgcggagcg ccatgccggc gtgctggcga cgctcaggcg
     4021 cgacgggcgc ccgcagcttt ccaacgtggt gtacgcgctg atcgacgggc gggtgcgcgt
     4081 gtcggtcacc gacacccgcg cgaaggtcgc caacctgcgc cgggaccccc gtgtgagcct
     4141 gcacgtgacc
//
LOCUS       DATMKC010000149         4807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_99667, whole genome shotgun sequence.
ACCESSION   DATMKC010000149 DATMKC010000000
VERSION     DATMKC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99667"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VNU01_04480"
     CDS             <1..1333
                     /locus_tag="VNU01_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31905.1"
                     /translation="AQAERARADRAEAELAARPPVVSGEPGSVFAEIGQQTQRILEAA
                     AEAADGIKQQAKREAEQEIRKARRQADKLIAEGERRREQLERGMADLVRQREELTTSL
                     RAVARGLEQSLRDLTLAEPGAATMREALRGDAGHTDVHTEAAVQPAQDVTPGAAVADD
                     LPAQDGTGAVEQAPAEAAAQPESEAQPQPEPEAQPEPEPEPEPEPRADAVAEVAAAPG
                     EPGEARARALEPLHPKVVRKLKRGLQDLQNIAYERLRAGGAAEPGAVLPPEEEFAPLG
                     EAVAEFLEQAYAAGGAEGARLAGRAPEEIKMPQELSRQFGADVAHRLNAPLAATLRMA
                     AAAREGMPALTDRVAGVFGEMKQTVADELAATHLVHAFEAGMHEAWAAAGVPARRWVL
                     GREPRCPEARCRTNDQSGPLRLGEEFPSGHEHPPVHAGCTCTTVPVVEARS"
     gene            1330..4197
                     /locus_tag="VNU01_04485"
     CDS             1330..4197
                     /locus_tag="VNU01_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWH31906.1"
                     /translation="MSAVQTLRKRWWVVVLAVAALALLFGARVAGFVTDVWWYQSIGF
                     VDVLWKLLGTQVLLGVVAALVVMVLVGANLLLARRLAPTYRIPSPQEESVERYRELLE
                     PFARPLMLAVAGLIGLFSGLAMAPRWETFLLFVNGVDFGVDDPQFGIDVGWFVFRLPF
                     WQLLNSWLFTSLAMTAVLAALAHYLFGGIRPQSPGRKLSPQASAHLSVLLAAMVAVRA
                     WGYLLDRYLLSYSERGQVAGLSYTDVHANLPALTLLAIIAVVVVILFLWNVRVRGWLL
                     PSAGVAIMLVASAVLQGVYPAAIQRLQVDPQELAREEPYIGRNLEFTRYGFGLRIGED
                     VQFEGFPAADTLQPDAAQRNERTLNSIRLWDPATLQRTYQQLQEFRPYYDFRDVDVDR
                     YSIAGRLEQTMISVREISEADLPAQAQTWQNRRLRYTHGFGLVASDVSAATGQGQPQF
                     YVRNIPPTGAEELVVENPRIYFGENPPEYSIVGTAMRELDYPVEGDVPRDFVYDGADG
                     VPAGSFLRRLAFALRYGEPNFVLSNLIEDESKVLFKRGIRERVEAVAPYLQLDHDPYP
                     VAVDGRIKWILDGYTSTDTLPYSERVNLAGLTLNQQRRFVPVATAEGGLELRERLVDV
                     PGLQGRANYIRNSVKAVVDAYDGTVTLYVIEPQDPLIQAWGRVFPDKLTPLEEASDEL
                     RAHFRYPEDMFRVQAALFRTYHIERADQFYTKEDAWEIPPDATSAANDGGDAQPMRPY
                     YLLMRLPGEEREEFALIQPFTPARKNNLTGWLAGRSDGEDYGQLKAFVMPPDRTVFGP
                     LQIQARVNQDARVAEQITLWNQSGSRVIYGNLLVIPVEDALLYAQPLFLRAETSEIPE
                     LNGVVLVLGDEVVMGETLAEALQLLFGETAGDDQGPEDDADDEPSTGVDPQVRELIER
                     ALSHFDAAERALREGDLATYQRETEAAEAALRQAREGEPPG"
     gene            4181..>4807
                     /locus_tag="VNU01_04490"
     CDS             4181..>4807
                     /locus_tag="VNU01_04490"
                     /inference="COORDINATES: protein motif:HMM:NF044700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31907.1"
                     /translation="MSHPADGQDPMVRVAFGPSETRHGAVGGAVAHVESDTVVELSRV
                     VPLLESAGFAVVTHTESRHDGATRHSFTLRAREGAVEGPGDARRLAAAVEACWHGWCE
                     VDALNALVLTAGVDWRDVAVLRAYRHYHRHLASALSGRTIDAALLANPAVAACLVAYF
                     RARFDGTADAAAAGAWHARAEVGHQAGGHRGVGEQRRVDGPPAERAGQV"
BASE COUNT          643 a         1708 c         1847 g          609 t
ORIGIN      
        1 ggcgcaggcc gagcgcgccc gcgccgaccg cgccgaggcc gagctggcgg cgcggccgcc
       61 ggtggtcagc ggcgagcccg gctcggtgtt cgccgagatc ggccagcaga cccagcgcat
      121 cctggaggcc gccgccgagg cggccgacgg catcaagcag caggccaagc gggaggccga
      181 gcaggagatc cgcaaggccc ggcgccaggc ggacaagctg atcgcggagg gtgagcgccg
      241 ccgcgagcag ctggagcgcg gcatggccga cctggtgcgc cagcgcgagg agctgaccac
      301 cagcctgcgg gcggtggcgc gcggcctgga gcagagcctg cgcgacctga ccctggccga
      361 gccgggcgcc gcgaccatgc gcgaggcgct gcgcggggac gccgggcaca ccgacgtgca
      421 caccgaggcc gcggtgcagc ccgcacagga cgtgacgccg ggtgcggcgg tcgccgacga
      481 cctgccggcc caggacggca ccggcgcggt ggagcaggcg ccggcggaag ccgccgcgca
      541 gccggagtcc gaggcgcagc cgcagccgga gcccgaggcg cagcccgagc ccgagcccga
      601 gcccgagccc gagccgcggg cggacgcggt ggccgaggtg gccgccgcgc ccggtgagcc
      661 cggcgaggcg cgcgcccggg cgctcgagcc gctgcacccc aaggtggtgc gcaagctcaa
      721 gcgcggcctg caggacctgc agaacatcgc ctacgagcgc ttgcgcgccg gcggggcggc
      781 cgagccgggc gcggtgctgc cgcccgaaga ggagttcgcg ccgctgggcg aggcggtcgc
      841 ggagttcctg gagcaggcgt acgccgcggg cggcgccgag ggcgcccggc tcgccggccg
      901 cgccccggag gagatcaaga tgccgcagga gctgtcccgt cagttcggcg ccgatgtcgc
      961 ccatcggctg aacgcgccgc tggccgccac gctgcgcatg gccgccgccg cgcgggaagg
     1021 gatgccggcg ctgaccgacc gggtggccgg cgtgttcggc gagatgaagc agaccgtcgc
     1081 cgacgagctc gcggccacgc acctggtgca cgcgttcgag gcgggcatgc acgaggcgtg
     1141 ggcggccgcc ggcgtgcccg cgcggcgctg ggtgctgggg cgggagccgc gctgccccga
     1201 ggcgcgctgc cgcaccaacg accagtccgg gccgctgcgc ctcggtgagg agttcccgtc
     1261 cgggcacgag cacccgccgg tgcacgccgg ctgcacgtgc acgaccgtcc ccgtggtgga
     1321 ggcgcgctcg tgagcgcggt ccagacgctg cgcaagcgct ggtgggtcgt cgtgctggcc
     1381 gtggcggcgc tggcgctgct gttcggcgcg cgggtcgccg gcttcgtcac ggacgtgtgg
     1441 tggtaccaga gcatcggctt cgtcgacgtg ctgtggaagc tgctcggcac gcaggtgctg
     1501 ctcggcgtgg tggcggccct ggtggtcatg gtgctggtcg gcgccaacct gctgctggcg
     1561 cgccggctgg cgcccaccta ccggatcccg tcgccgcagg aggagagcgt cgagcgctac
     1621 cgcgagctgc tggagccgtt cgcgcggccg ctgatgctgg cggtcgccgg gctgatcggg
     1681 ctgttctccg gccttgcgat ggcgccccgc tgggaaacgt tcctgctgtt cgtcaacggc
     1741 gtggacttcg gcgtggacga cccgcagttc ggcatcgacg tcggctggtt cgtgttccgc
     1801 ctgccgttct ggcagctgct gaactcctgg ctgttcacct cgctggcgat gacggcggtc
     1861 ctggctgccc tggcgcacta cctgttcggc ggcatccgcc cgcagtcgcc ggggcgcaag
     1921 ctgtcgccgc aggccagcgc gcacctgtcg gtgctgctgg cggcgatggt ggcggtgcgg
     1981 gcgtggggct acctgttgga ccgctacctg ctgtcctaca gcgagcgcgg ccaggtcgcg
     2041 ggcctgtcct acacggacgt gcacgcgaac ctgccggcgc tgacgctgct ggcgatcatc
     2101 gccgtggtgg tggtgatcct gttcctgtgg aacgtgcgcg tgcgcggctg gctgctgccg
     2161 tccgccggcg tggcgatcat gctggtggcc tccgccgtgc tccagggcgt gtacccggcg
     2221 gcgatccagc gcctgcaggt ggatccgcag gaactggccc gcgaggagcc ctacatcggg
     2281 cgcaacctgg agttcacgcg ctacggcttc ggcctgcgca tcggcgagga cgtgcagttc
     2341 gagggcttcc cggccgcgga cacgctgcag ccggacgccg cccagcgcaa cgagcgcacg
     2401 ctgaactcca tccggctgtg ggacccggcg acgctgcagc gcacctacca gcagctgcag
     2461 gagttccggc cgtactacga cttccgcgac gtcgacgtcg accgctacag catcgccggg
     2521 cggctggagc agaccatgat ctccgtgcgg gagatctccg aggcagacct gcccgcccag
     2581 gcgcagacgt ggcagaaccg ccgcctgcgc tacacgcacg gcttcggcct ggtcgccagc
     2641 gacgtgagcg ccgccacggg ccagggccag ccgcagttct acgtgcgcaa catcccgccc
     2701 accggcgccg aggaactggt ggtggagaac ccgcgcatct acttcggcga gaacccgccg
     2761 gagtactcca tcgtgggcac cgcgatgcgc gagctggact acccggtgga gggcgacgtc
     2821 ccgcgcgact tcgtctacga cggcgccgac ggcgtgccgg ccggctcctt cctgcggcgc
     2881 ctggcgttcg cgctgcgcta cggcgagccg aacttcgtgt tgtccaacct catcgaggac
     2941 gagtccaagg tcctgttcaa gcgcggcatc cgcgagcgcg tggaggcggt ggcgccgtac
     3001 ctgcagctgg accacgaccc gtaccccgtg gcggtggacg ggcgcatcaa gtggatcctg
     3061 gacggctaca ccagcacgga cacgctgccg tactccgagc gggtgaacct ggcggggctg
     3121 acgctcaacc agcagcgccg cttcgtcccc gtggccacgg ccgagggcgg cctggagctg
     3181 cgcgagcgcc tggtggacgt gccgggcctg cagggccgcg ccaactacat ccgcaacagc
     3241 gtgaaggcgg tggtcgacgc ctacgacggc acggtgacgc tgtacgtcat cgagccgcag
     3301 gacccgctga tccaggcgtg gggcagggtg ttccccgaca agctcacgcc gttggaggag
     3361 gccagcgacg agctgcgcgc ccacttccgc taccccgagg acatgttccg ggtgcaggcg
     3421 gcgctgttcc gcacgtacca catcgagcgg gccgaccagt tctacaccaa ggaggacgcc
     3481 tgggagatcc cgccggacgc cacgtccgcg gccaacgacg gcggcgacgc gcagcccatg
     3541 cgcccgtact acctgctgat gcggctgccc ggcgaggagc gcgaggagtt cgcgctgatc
     3601 cagccgttca cgccggcgcg caagaacaac ctgaccggct ggctggccgg tcgcagcgac
     3661 ggcgaggact acgggcagct caaggcgttc gtcatgccgc cggaccgcac cgtgttcggt
     3721 ccgctgcaga tccaggcgcg cgtgaaccag gacgcgcgcg tggccgagca gatcacgctg
     3781 tggaaccagt ccggttcgcg cgtcatctac ggcaacctgc tggtgatccc ggtggaggac
     3841 gcgctgctgt acgcccagcc gctgttcctg cgcgccgaga ccagcgagat ccccgagctc
     3901 aacggcgtcg tgctggtgct gggcgacgag gtggtgatgg gcgagaccct ggcggaggcg
     3961 ctgcagctgc tgttcggcga gaccgccggc gacgaccagg ggccggagga cgacgcggac
     4021 gacgagccgt ccaccggcgt ggacccgcag gtgcgcgagc tgatcgagcg ggcgctcagc
     4081 cacttcgacg ccgccgaacg cgcgctgcgc gagggcgacc tggccaccta ccagcgcgag
     4141 accgaggccg cggaggcggc gctgcgccag gcccgcgagg gtgagccacc cggctgacgg
     4201 gcaggacccc atggtgcgag tcgcgttcgg acccagcgag acccggcacg gcgccgtcgg
     4261 cggggccgtg gcgcacgtcg agtccgacac cgtggtcgag ctgtcgcgcg tggtgccgct
     4321 gctggagtcc gccggcttcg cggtggtgac ccacaccgag tcgcgccatg acggcgcgac
     4381 gcggcacagc ttcacgctgc gcgcgcgcga gggcgcggtg gagggccccg gcgacgcgcg
     4441 gcggctggcc gccgcggtgg aggcgtgctg gcacggctgg tgcgaggtgg acgccctcaa
     4501 cgcgctggtg ctcaccgccg gggtggactg gcgcgacgtc gcggtgctgc gcgcctaccg
     4561 ccactaccac cgccacctgg ccagcgcgct cagcgggcgg accatcgacg cggcgctgct
     4621 cgccaacccc gcggtggccg cctgcctggt ggcctacttc cgcgcgcgct tcgacggcac
     4681 ggcggacgcg gccgcggctg gggcctggca cgcgcgcgcg gaagtaggcc accaggcagg
     4741 cggccaccgc ggggttggcg agcagcgccg cgtcgatggt ccgcccgctg agcgcgctgg
     4801 ccaggtg
//
LOCUS       DATMKC010000150         4631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_265348, whole genome shotgun sequence.
ACCESSION   DATMKC010000150 DATMKC010000000
VERSION     DATMKC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265348"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VNU01_04495"
     CDS             <1..481
                     /locus_tag="VNU01_04495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH31908.1"
                     /translation="FHDRLRWEDQMPEGPHLWRTLGYSRTLWEPGRTAIQWEAAQDYC
                     FPANGGYIVHGGMVTTILDSAMGGACWSVLDHHEAFLTADLRVEFLRAAAPGTLVAEG
                     WVVRRTRRVVFCAAELYDAGGQLLATSRCTQVVLPADGTAGRYSATDRDTGGGTDEE"
     gene            complement(433..1461)
                     /locus_tag="VNU01_04500"
     CDS             complement(433..1461)
                     /locus_tag="VNU01_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWH31909.1"
                     /translation="MDASTVALTLLALALGALAGAALARPRPPARPAGPAADADLATL
                     TAELRRLQDVVRQAEQGRAETYGLLSAQVRAAGEQTARLAETTGALREALSSTKARGQ
                     WGERMAEDVLRLAGLVEGVNYTKQSAVAGGGVPDFTFLLPRGLSLHMDVKFPLDNYLR
                     YLNAEHDAERESARQQFLRDVRVCVRGLAARAYADPSASVDCVLLFIPNEQLFAFVQE
                     QDPGILDDALARKVVPCSPLTLFAVLAVVRQAAENFALARTSNEILVLLSRFAGQWER
                     YTTELDKLGRQMETARRTYDELAGPRRRQLERPLDQLDDLRRARGLEGPEDYSSSVPP
                     PVSLSVAE"
     gene            1506..2606
                     /locus_tag="VNU01_04505"
     CDS             1506..2606
                     /locus_tag="VNU01_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWH31910.1"
                     /translation="MCPDSTTLVLPAYGGAWIGGVLPALLGEGRRDLLPPPARDARAV
                     VVLALDGLGWAMLEQHRDVVPNLAAMAGGPVTTVAPSTTSAALTSITTGMPPGAHGIV
                     GYRMRVGGEVLNVLRWQAGEHSPEPDRVQPRPPFLGVRVPVVTKQEFRSSAFSAAHLR
                     GGRFTGYRTVSSLVEHCRLLVAAGERVVYAYSDGIDKVAHEYGLRDGFLQAELRATDA
                     LVGQLLDALPSSSALVVLADHGQVHVEPDGHRSLEPVERLVAQSAGEGRFRTLWARPG
                     ASAELLVAARELYGGEAWVRSRDEVVAEGWLGPDVSAEVRGRIGDVVLAAREPVIFVD
                     KAMPLEVQMKSHHGSLTDEEMLVPLIAAPGRA"
     gene            complement(2603..3007)
                     /locus_tag="VNU01_04510"
     CDS             complement(2603..3007)
                     /locus_tag="VNU01_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWH31911.1"
                     /translation="MRELVLDASAVVHMLTGAQPWVPRAVIAQAALSSPSHLDAEVLS
                     ALARMHRAGLLTDTRVAQALTDLAAMPINRLPISQLQLLSAWALRHNVAVKDALYLAL
                     ANDRGTKVVTGDQRLRRAVPELTVGPDELAAG"
     gene            complement(3004..3279)
                     /locus_tag="VNU01_04515"
     CDS             complement(3004..3279)
                     /locus_tag="VNU01_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31912.1"
                     /translation="MPDMLIRNVPEAVQDVLKRRAKEAGMSVQRYVVKLLEEHTERMT
                     MREWLDMVGQRPKVPGLDAAQAVREAREEDDRLWDEHLRDRYPEAFK"
     gene            complement(3371..>4631)
                     /locus_tag="VNU01_04520"
     CDS             complement(3371..>4631)
                     /locus_tag="VNU01_04520"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HWH31913.1"
                     /translation="VPLALGTDTAGSGRVPAAMNGIAGWKPTRGIVSATGVVPACRSL
                     DCVSVFAGTAADLRAAVDVLARHDPDDAHARTGAETLGPAAAVAGLRVGVPDLAGLDV
                     DAGTRASLGEAVRRLSGLGVEITPVDLSGVLEAGRLLYHGPWLAERLEAAGELMERDD
                     GALLDVTRRVLAAGREYRAVDVFRAQTRVRVHAAACERLWRAVDILVVPSVPAAFTLA
                     EVAADPIGVNERLGTFTNFVNLLDLAAVAMPVGLRADGVPAGVTLVGPALTDLTLLAV
                     ARAFAGGDGMPEPRPDGIPLAVVGAHLRGQPLHHELRDAGAHFLQRTRTAPRYRLYAL
                     SGTAPPKPGMVEVHADGAALEVEVYTLAADAFGRFVAGVRSPLYIGPVELADGRVVSG
                     FRCGTEATAGSTDITRFGGWRAYLAAR"
BASE COUNT          605 a         1675 c         1737 g          614 t
ORIGIN      
        1 gttccacgac cggctgcgct gggaggacca gatgccggag ggcccgcacc tgtggcggac
       61 cctgggctac agccgcacgc tgtgggagcc ggggcgcacc gcgatccagt gggaggcggc
      121 gcaggactac tgcttcccgg ccaacggcgg ctacatcgtg cacggcggca tggtgaccac
      181 cattctcgac agcgcgatgg gcggcgcctg ctggagcgtg ctggaccacc acgaggcgtt
      241 cctcaccgcc gacctgcggg tggagttcct gcgtgcggcc gcgcccggca cgctcgtcgc
      301 cgaaggctgg gtcgtccggc gcacccgccg cgtggtgttc tgcgcggcgg agctgtacga
      361 cgcgggcgga cagcttttgg cgacgtcgcg ctgcacgcag gtggtgttgc ccgccgacgg
      421 gaccgccggg cgctactcgg ccacggacag ggacaccggc gggggcacgg acgaggagta
      481 gtcctccggc ccttccagcc ccctggcgcg gcgcaggtcg tcgagctggt ccagcggccg
      541 ctccagctgg cgccgccgcg gccccgccag ctcgtcgtag gtgcggcgcg cggtctccat
      601 ctggcggccg agcttgtcga gctcggtggt gtagcgctcc cactggccgg cgaagcgcga
      661 cagcagcacc aggatctcgt tggaggtgcg cgccagggcg aagttctcgg cggcctggcg
      721 caccactgcg agcaccgcga acagggtcag cggcgagcac ggcaccacct tgcgcgccag
      781 cgcgtcgtcg aggatcccgg ggtcctgctc ctgcacgaac gcgaacagct gctcgttggg
      841 gatgaacagc agcacgcagt ccaccgacgc ggacgggtcc gcgtaggcgc gcgccgccag
      901 gccgcgcacg cagacgcgca cgtcgcgcag gaactgctgg cgagcggact cgcgctcggc
      961 gtcgtgctcg gcgttgaggt agcgcaggta gttgtccagg gggaacttca cgtccatgtg
     1021 cagcgacagg ccgcgcggca gcaggaacgt gaagtccggc acgccgccgc cggccaccgc
     1081 cgactgcttg gtgtagttga cgccctcgac aaggccggcc aggcgcagga cgtcctccgc
     1141 catgcgctcg ccccactggc cgcgcgcctt ggtgctcgac agggcttcgc gcagcgcgcc
     1201 ggtggtctcc gccaggcgcg cggtctgctc gccggcggcg cgcacttgcg cgctgagcag
     1261 gccgtaggtc tcggcgcgac cctgctcggc ctgccggacc acgtcctgca gccgccgcag
     1321 ctccgcggtg agcgtggcca gatcggcgtc ggccgcgggt cccgccgggc gcgccggcgg
     1381 tcgcgggcgc gcgagggcgg cgccggccag ggcgccgagc gccagggcga gcagcgtgag
     1441 ggcaaccgtg gaggcatcca tggggatcac gctacgcggg gggtgtgacg ggtacggtgc
     1501 cgggcatgtg tccggactcc acaaccttgg tgctgcccgc gtacggcggc gcctggatcg
     1561 gcggcgtgct ccccgcgctg ctgggcgagg ggcgtcgcga cctgctgccg ccgccggcgc
     1621 gcgatgcccg cgcggtcgtc gtgctcgcgc tcgacgggct gggctgggcc atgctggaac
     1681 agcaccgcga cgtcgtgccg aatctcgcgg ccatggccgg cgggccggtc accacggtgg
     1741 cgccgtcgac gaccagcgcc gccctgacgt ccatcaccac ggggatgccg cccggggcgc
     1801 acggcatcgt gggctaccgc atgcgggtgg gcggcgaggt gctgaacgtg ctgcgctggc
     1861 aggccggcga gcacagcccg gagccggacc gcgtccagcc gcggccgccg ttcctgggcg
     1921 tgcgcgtgcc cgtggtgacc aagcaggagt tccgctccag cgcgttcagc gccgcacacc
     1981 tgcgcggcgg gcgcttcacc ggctaccgga cggtgtcgtc gctggtggag cactgccggc
     2041 tgctggtcgc cgccggcgag cgcgtggtgt acgcctacag cgacggcatc gacaaggtcg
     2101 cccacgagta cgggctgcgc gacggcttcc tgcaggccga gctgcgcgcc accgacgcgc
     2161 tggtcgggca gctcctggac gcgctgccgt cgtcgtcggc gctggtggtg ctggccgacc
     2221 acggccaggt ccacgtcgag ccggacggcc accgctcgct ggagccggtg gagcggctgg
     2281 tcgcgcagtc ggccggcgaa gggcgcttcc gcacgctgtg ggcgcgcccc ggcgcgtcgg
     2341 cggagctgct cgtcgccgcg cgggagctgt acggcggcga ggcctgggtc cgctcccgcg
     2401 atgaggtcgt ggcggagggc tggctcggcc cggacgtgag cgccgaggtg cgcgggcgca
     2461 tcggcgacgt ggtgctcgcc gccagggagc cggtgatctt cgtggacaag gcgatgccgc
     2521 tggaggtgca gatgaagtcg catcacggca gcctcaccga cgaggagatg ctggtgccgt
     2581 tgatcgccgc gcccggccgc gcctagcccg ccgccagctc gtccggcccg accgtcagct
     2641 ccggcaccgc ccgccgcagc cgctggtcgc ccgtcaccac cttggtcccc cggtcgttcg
     2701 ccagcgcgag gtagagcgca tctttgaccg ccacgttgtg acggagcgcc cacgcggaga
     2761 gcaactggag ctggctgatc gggaggcggt tgatcggcat ggcggccaga tcggtgaggg
     2821 cctgcgccac cctggtgtcg gtgaggagcc ccgctcgatg catgcgcgcc agcgcggaca
     2881 gcacttccgc gtccaggtgg gatggagagg acagagcggc ttgcgcgatg accgctctgg
     2941 ggacccaggg ttgcgctccg gtcagcatat ggacgacagc cgaggcgtcc agcaccagtt
     3001 ccctcacttg aacgcctcgg ggtatcggtc gcgaaggtgc tcatcccaaa ggcggtcgtc
     3061 ctcctcgcgg gcttcccgga ctgcctgagc ggcgtcgagt ccgggcactt tcggccgttg
     3121 ccccaccatg tccagccact cgcgcatcgt catgcgctcg gtgtgctcct ccagcagctt
     3181 gacgacgtag cgctgcacgc tcatgccggc ctccttcgcc cgccgcttga gcacgtcctg
     3241 gaccgcctcc gggacgttgc ggatcagcat gtcgggcatg gaacacctcg ccggctcgtc
     3301 gtgccaccgc agcgaccgtt tgcctgtgga tagcaaaacg atagcgccgc gtccgcgggg
     3361 cggccagggc tcagcgggca gccagatacg cgcgccagcc gccgaatctc gtgatgtccg
     3421 tcgagccggc cgtcgcctca gtgccgcagc ggaacccgct gaccacccgg ccgtcggcga
     3481 gctcgaccgg tccgatgtac agcggggagc gcacaccggc gacgaagcgt ccgaacgcgt
     3541 ccgcggcgag cgtgtagacc tccacctcga gcgcagcgcc atcggcgtgc acctcgacca
     3601 tgcccggctt cggcggcgcc gtgccggaca gcgcgtacag ccggtagcgc ggcgcggtcc
     3661 gggtgcgctg caggaagtgc gcgcccgcgt cgcgcagctc gtggtgcagg ggctgcccgc
     3721 gaagatgggc gccgacgaca gccagcggga tcccgtccgg ccgcggctcc ggcatcccgt
     3781 cgccgccggc gaacgcgcgt gccaccgcca ggagcgtcag gtcggtgagg gccggtccga
     3841 ccagggtcac gccggcgggc acgccgtccg cgcgcaagcc gacgggcatg gcgacggccg
     3901 ccagatccag cagattgacg aagttggtga acgtgcccag tcgctcgttc acaccgatcg
     3961 gatccgcggc gacctccgcg agggtgaacg ccgccggtac ggacggcacg accaggatgt
     4021 cgaccgcccg ccagagacgt tcacaggcgg cggcgtgaac tcgcacgcgc gtctgcgcgc
     4081 ggaagacgtc cacggcgcgg tactcgcgtc ccgcggccag cacccgccgg gtgacgtcga
     4141 gcagcgcgcc gtcgtcccgc tccatcagct cgcccgccgc ctccagccgc tcggcgagcc
     4201 atggcccgtg atagagcagc cgcccggctt ccagcacgcc gctgaggtcg acgggggtga
     4261 tctccacgcc gaggccggac agccgtcgca ccgcctcgcc caggctcgcg cgagtcccgg
     4321 catcgacgtc cagtcctgcc aggtccggca cgcccacgcg caggcccgcc accgccgcgg
     4381 ccggccccag tgtctccgcg ccggtgcgcg cgtgtgcgtc gtccgggtcg tgccgggcga
     4441 gcacgtccac ggcggcacgc aggtctgcgg cggtgccggc gaagacggac acgcagtcca
     4501 gcgatcggca ggcggggacc acgccggtgg cggagacgat cccgcgcgtg ggtttccatc
     4561 cggcgatgcc gttcattgcg gcggggacgc ggccggaacc agccgtgtcg gtgccgagcg
     4621 cgagcggcac c
//
LOCUS       DATMKC010000151         5377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_27996, whole genome shotgun sequence.
ACCESSION   DATMKC010000151 DATMKC010000000
VERSION     DATMKC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27996"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..897
                     /locus_tag="VNU01_04525"
     CDS             262..897
                     /locus_tag="VNU01_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31914.1"
                     /translation="MPKVEISNAVRADHEEVRTILLDAFAGAPLGVEIHVKARRPRVT
                     WRAICNALDCYPPNWSGAGANYHWFTTRKDLERVATRERHGPVERVVSPAGFSGRAYS
                     GVPRIANVAPGTAYLVTLHMPSDTAHAGPYPALRKYPGLKTAPPIPVGCWREELFAVA
                     AHEAFHIQQFATGARVSEIDAEKAALAALAAWREPPISAVGSRDPVPLRLF"
     gene            1088..3655
                     /locus_tag="VNU01_04530"
     CDS             1088..3655
                     /locus_tag="VNU01_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWH31915.1"
                     /translation="MRDAVVLPALTAHHLLLVAGRAAAERDRDRLVVQACEQARLALR
                     ASWAVLARIDEDGRLVAVAGSGSVPRGTVLAGSAAVADAGLSSATGPVRLTAGEDGVP
                     ADAVMIDPDRGEAGVLVAARSAVSGDALSQDDRVFLVQLARLLAGAFERIRLDQEIAA
                     NEQRLAEAQQLARLGSYDWHIPTDTNTWSDELYRIYGYEPGAFNASYERFLSLIHPDD
                     RERIVAIHTKAMEDHQPYRMEERIVRPDGDVRILDSTGKVVLDEHGRPVRMVGICLDV
                     TEQRRAAEALRRSQERFRDLVESAPDAVILFDADGTILQANRETERLFGWSAGDLIGV
                     SVESLLPADVRRPSGLSLDLMGRHRNGTPVPVDVSLARIETDDGAATAAFVRDATARK
                     LAEAAQQRLAQAEERRRQALEINDNVVQGLTGVVGALEMGWTQEAASAARATLASASA
                     MMSELLGMGQRALEPGALVRGDAAPTMRESAMSVPPRPAAPVDRRAHEPGAPPLTVLL
                     ADDSADLRALYRLMLSRHGIAVVAEAGDGVEAVRQAVEHRPDIALVDLAMPGMDGLQV
                     AEALRQQLPDCRVVMLSGFEESRVAEQARAAGVERYVAKGVDADTLAAALYEVGPPDR
                     RGHHARGAVVPVPDPVDELLHELRTPLTVIAGIAATLDDRLDDTPPGEVHELLEAVSR
                     NAEHMGNLVRAYADMQRLHSGRLPLAPEPVPLDALVRESVADMRRMLESHEVHYRVGE
                     PVVARADPLRVRQILTNLLSNAAKFSPPGSRIEVEVASVDGMAELRVRDSGPGIPPER
                     RPLLFRPFERLGAPGKGAGLGLYISRGLAEAHGGSLRLGEDTPDGLTTFVLRLPAAT"
     gene            complement(3621..>5377)
                     /locus_tag="VNU01_04535"
     CDS             complement(3621..>5377)
                     /locus_tag="VNU01_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HWH31916.1"
                     /translation="SYRHLADYERELRDLAEDNPGLVRLVTLPHTSLEGREVLGVEIS
                     RDVDATGDGKPTFLMMGLHHAREWPSGEHSMEFALDLVQHAGDERIGSLLERTRVVVV
                     PVVNPDGFDQSRKWGDIADLRQVDDELAAHGEDTGGLVGVLGTPGNAYKRKNCRLWDD
                     QPTPPLSCEAPSPGGFGTGIDLNRNYGGLWGGGGSSDLFADPDYRGPAPFSEPETQNV
                     RDFVSSRHVTTLITNHTYSNLILRPPGTRAAGETVDEAAMKDLGARMAAQNGYRNIHG
                     WQLYDTDGTTEDWSYNATGGYGYTFEIGPDEFHPPFSETVDQYVGHGFYAGKGNREAF
                     LLALENAADPAHHAVLSGKAPDGAVLRLVKEFDTKTSDPALTVDDRLESVLHVTGNRP
                     FEWHVNPSTRPEVMEHRVRVLAETPTREEVVEHTEAGWRPGDHVDFDFPMPEEGVGAL
                     KVNLDWPTPDDLDLYVYQKGPGGELIEVGSSGNFIGDKEEAIVDLPDAGDYVIRVVNY
                     ASVSPTFTLTAGLYPVDREEVFGGGVVESYTLTCETPDGDVLQTAQVTVDRGQAQKLD
                     LKECARAFRSPPAGAGRT"
BASE COUNT          728 a         2001 c         1907 g          741 t
ORIGIN      
        1 tggacaacct cgtcctggtc tgctggcgct gccacagcca catccaccaa cacggctggc
       61 aagtgtggaa ccacaacggc cgctacaccg ccgggccacc aggccaagca ccaccgcacc
      121 ttgaaaacaa catagagaaa cggctgctcg agcatgcgta ctgacccgtc acccctccgc
      181 gcggtggggt gcggagcctg cctggcgggc ggcggtgggc gctcggcgga ggtgtcgcac
      241 gggtgcgcta ccttcgccgc gatgccgaag gtcgagatca gcaacgcagt gcgtgcggac
      301 cacgaggagg tccgcaccat cctgctggac gccttcgccg gcgcgccgct cggtgtcgag
      361 atccacgtca aggcgcggcg accccgcgtg acgtggcggg cgatctgcaa cgcgctcgac
      421 tgctatccgc cgaactggtc gggagccggg gcgaactacc actggttcac cacgcgcaag
      481 gacctggagc gagtggcgac cagggaacgg cacgggccgg tcgaacgcgt cgtcagcccg
      541 gcggggttct ccgggcgggc gtacagcggt gtcccgcgca tcgcgaacgt cgcgcccggc
      601 accgcctacc tggtgacgct ccacatgccc agcgacaccg cgcacgccgg gccctacccc
      661 gcgctgcgga agtaccccgg cctgaagacg gcgccgccga tcccggtcgg gtgctggcgg
      721 gaggagctct tcgcggtcgc cgcgcacgag gcgttccaca tccagcagtt cgcgaccggc
      781 gcccgcgtga gcgagatcga cgcggagaag gccgcgctcg ccgcgctcgc cgcctggcgg
      841 gagccgccga tctcagcagt cgggagccga gacccggttc ccctccggct cttctgacag
      901 ccccgagcag aggcgcacta cctgcggttc cagcttgcgg gcccgcacgt catctgccgc
      961 caagccgcgg tgccctagtc cgaactggcc atggcgcgcg gcgggaggtc gaggccacac
     1021 tatccggggg ggttgcggct ggcgcccgtg ttcgggcgcc ttaccagtgg agcgtgcctt
     1081 gcctgcgatg cgcgacgccg tggtcctgcc ggcgctgacc gcacaccatc tcctgctcgt
     1141 cgccggacgc gcggccgccg agcgcgaccg cgaccgcctc gtcgtgcaag cctgcgagca
     1201 ggctcgcctt gcccttcgcg cctcgtgggc ggtcctggcg cgcatcgacg aggacggcag
     1261 gctggtggcg gtggcaggct cgggcagcgt gccgcgcggc accgtgctgg ccggctcggc
     1321 ggcggtcgcc gacgcgggcc tgtcctcggc caccgggccc gtacggctca cagccggcga
     1381 ggacggggtg ccggccgacg ccgtgatgat cgacccggac cgcggcgaag cgggcgtgct
     1441 ggtcgccgca cgctccgcgg tctccgggga cgcgctcagc caggacgacc gggtcttcct
     1501 ggtgcagctc gcgcggctgc tcgccggcgc gttcgagcgc atccgcctgg accaggagat
     1561 cgcggcgaac gagcagcgcc tggcggaggc gcagcagctc gcgcggctgg gcagctacga
     1621 ctggcacatc cccacggaca ccaacacgtg gagcgacgag ctctaccgca tctacgggta
     1681 cgagcccggc gccttcaacg cctcctatga gcggttcctg tcgctcatcc accccgacga
     1741 ccgtgaacgc atcgtcgcca tccacaccaa ggcgatggag gaccaccagc cgtaccgcat
     1801 ggaggagcgc atcgtccgcc ccgatggcga cgtccgcatc ctggactcca ccgggaaggt
     1861 cgtgctggac gagcacggcc ggccggtgcg gatggtgggc atctgcctcg acgtcaccga
     1921 gcagcgccgc gcggccgagg cgctgcgccg cagccaggag cgcttccgcg acctggtcga
     1981 atcggcgccc gacgccgtga tcctgttcga cgcggacggc accatcctcc aggcgaaccg
     2041 ggagaccgag cgcctgttcg gatggagcgc gggcgacctg atcggagtga gcgtcgagtc
     2101 gctgctgccc gccgacgtcc gccggccctc gggcctgagc ctcgacctga tgggccggca
     2161 ccgcaacggc acgcccgtgc ccgtcgacgt cagcctcgcg cgcatcgaga ccgacgacgg
     2221 tgccgccacg gccgcgttcg tccgcgacgc cacggcgcgc aagctcgccg aggccgcgca
     2281 gcagcgcctg gcccaggccg aggagcgccg gcgccaggcg ctcgagatca acgacaacgt
     2341 cgtgcagggc ctcaccggcg tggtcggcgc actggagatg ggatggacgc aggaagccgc
     2401 gtccgcggcg cgggcgaccc tggcgtcggc gagcgccatg atgagcgagc tgctcggcat
     2461 ggggcagcgg gctctggagc cgggcgcgct cgtccgcggg gacgccgcgc ccaccatgcg
     2521 cgaatccgcg atgagcgtgc cgccgcgtcc cgcggccccc gtcgaccgcc gcgcgcacga
     2581 accgggtgcc ccgccgctga cggtgctgct cgcggacgac agcgccgacc tgcgcgcgct
     2641 gtaccggctg atgctgtcgc gtcacggcat cgcggtcgtg gccgaggccg gcgacggcgt
     2701 cgaagccgtg cgccaggcgg tggagcaccg gcccgacatc gccctggtgg acctcgccat
     2761 gcccggcatg gacggcctgc aggtggccga ggccctgcgg cagcagctcc cggactgccg
     2821 cgtcgtcatg ctgtccggct tcgaggagtc ccgcgtcgcc gagcaggcac gcgcggccgg
     2881 tgtcgagcgc tacgtcgcca agggcgtgga cgccgacacg ctcgcggccg cgctgtacga
     2941 agtcggcccc ccggaccgcc gcggccacca cgcccgcggc gcggtcgtgc cggtaccgga
     3001 cccggtcgac gagctgctgc acgagctgcg cacgccgctc accgtcatcg ccggcatcgc
     3061 cgcgacgctc gacgaccgcc tcgacgacac gccgcccggc gaggtgcacg agctgctcga
     3121 agcggtcagc cgcaacgccg agcacatggg caacctggtg cgcgcgtacg ccgacatgca
     3181 gcgcctgcac tccgggcgcc tgccgctggc gccggaaccc gtgccgctcg acgccctggt
     3241 gcgcgagtcc gtcgccgaca tgcggcgcat gctcgagtcc cacgaggtgc actaccgggt
     3301 cggcgagccc gtcgtcgccc gggccgaccc gctcagggtc cgccagatcc tcaccaacct
     3361 gctgtccaac gccgcgaagt tctcacctcc cggctcgcgc atcgaagtcg aggtcgccag
     3421 cgtggacggc atggcagaac tgcgcgtgcg cgacagcggc ccgggcatcc ccccggagcg
     3481 ccggccgctg ctgttccggc cgttcgaacg gctgggtgcc cccggcaagg gcgcagggct
     3541 cggcctgtac atctcccggg gtctcgccga ggcgcacggc ggatcgctgc gcctcggcga
     3601 ggacaccccg gacgggctga ctacgttcgt cctgcgcctg ccggcggcga cctgaacgcg
     3661 cgcgcgcact ccttcaggtc cagcttctgg gcctggccgc ggtccaccgt cacctgggcc
     3721 gtctggagca cgtccccatc gggcgtctcg cacgtgagcg tgtaggactc caccacgccg
     3781 ccgccgaaga cctcctcacg gtccaccggg tacagcccgg cggtgagcgt gaacgtcggc
     3841 gagaccgagg cgtagttcac cacgcggatc acgtagtccc cggcgtcggg caggtcgacg
     3901 atggcctcct ccttgtcccc gatgaagttg ccggagctgc ccacctcgat cagctcgccg
     3961 cccgggccct tctggtacac gtacaggtcc aggtcgtccg gcgtcggcca gtcgaggttc
     4021 accttcagcg cgccgacgcc ctcctccggc atcgggaagt cgaagtcgac gtggtcgccg
     4081 ggccgccagc cggcctcggt gtgctcgacg acctcctcgc gggtcggcgt ctcggccagg
     4141 acgcgcacgc ggtgctccat gacctccggc cgcgtcgacg ggttcacgtg ccactcgaac
     4201 ggccggttgc cggtcacgtg caggaccgac tccaggcggt cgtccacggt cagggccggg
     4261 tcggacgtct tcgtgtcgaa ctccttcacc aggcgcagca ccgccccgtc gggcgccttg
     4321 ccgctcagca ccgcgtggtg cgccgggtcg gcggcgttct ccagcgcgag caggaacgcc
     4381 tcgcggttgc ccttgccggc gtagaagccg tggccgacgt actggtccac ggtctcgctg
     4441 aacggcgggt ggaactcgtc agggccgatc tcgaacgtgt agccgtagcc gccggtggcg
     4501 ttgtacgacc agtcctcggt ggtgccgtcc gtgtcgtaga gctgccagcc gtggatgttg
     4561 cggtagccgt tctgcgcggc catgcgcgcg cccaggtcct tcatcgcggc ctcgtccacc
     4621 gtctcgccgg cggcccgcgt ccccggcggg cgcaggatca ggttcgagta cgtgtggttg
     4681 gtgatcagcg tggtcacgtg gcgggaggag acgaagtcgc ggacgttctg cgtctccggc
     4741 tcggagaacg gcgccggccc gcggtagtcc gggtccgcga acaggtccga ggagccgccg
     4801 cctccccaca gcccgccgta gttgcggttc aggtcgatgc ccgtcccgaa cccgccgggg
     4861 ctgggtgcct cgcaggacag cggcggcgtg ggctggtcgt cccacagccg gcagttcttg
     4921 cgcttgtagg cgttgccggg cgtgcccagg acacccacca ggccgccggt gtcctcaccg
     4981 tgggccgcaa gctcgtcgtc cacctggcgc aggtccgcga tgtcgcccca cttgcggctc
     5041 tggtcgaagc cgtccgggtt gaccaccggg accaccacga cgcgcgtgcg ctccagcagc
     5101 gagccgatgc gctcgtcgcc ggcgtgctgg accaggtcca gcgcgaactc catggagtgc
     5161 tcgccggacg gccactcgcg cgcgtggtgc aggcccatca tcaggaacgt cggcttgccg
     5221 tccccggtcg cgtccacgtc gcggctgatc tccacgccga ggacctcacg accctccaag
     5281 ctggtgtggg ggagcgtgac cagtcgcacc aggccggggt tgtcctccgc caggtcgcgc
     5341 agctcccgct cgtagtcggc gaggtgccgg tagctgt
//
LOCUS       DATMKC010000152         3435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_188126, whole genome shotgun sequence.
ACCESSION   DATMKC010000152 DATMKC010000000
VERSION     DATMKC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188126"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VNU01_04540"
     CDS             complement(<1..932)
                     /locus_tag="VNU01_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWH31917.1"
                     /translation="MTSPFGLLAILALILGNAYFVAAEFAFVAVRRSHLEERAAEGDP
                     RAARAVRVVQRLSFVLSGAQLGITATSLVLGFIATPIIAELLAPALTLARVPEGARGG
                     LAATVALVLATMLQMVVGELAPKNLAIARPETFASRLAAPTLLYTRLAGWLIRIFDSS
                     ANALLRALGIEPVEHLETGVSPEELELIIVESSSGGHLTADQAQLLTRALEFRQLRAA
                     DVMVPRPQVSSVPADATGADLRAVAASSGHSRFPVVDADRDEILGVVQAKDLLRHAPG
                     DRDGVPVRTLMSRALAVPESAALSAVVAEMRRARS"
     gene            complement(945..2822)
                     /locus_tag="VNU01_04545"
     CDS             complement(945..2822)
                     /locus_tag="VNU01_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HWH31918.1"
                     /translation="MVDTPQPSFRSAALARARGYAVPALRVVGIAFGVMLLLTFGLRV
                     ARAGALPGVHLDGQDVSGMNGARLHDLVSDLARERTAQSVTVVRAPVPSAGATEEATV
                     AATGAELGYSLDVDGTVKAVLRRGRQLNPFAAFADQVRAFFGTTTVEAVQEVDQERLS
                     DWLEQAQRRLRVDPIEGSLAFEGASVARVEPKPGADVRRDALREDVLDALFHGRSEVR
                     ARTAPLEPLTTSADVDEVEEAARRALAASVELRRGDVTLTLTPEDLGAVLTVERVTHD
                     DEVSLELRVSPEALEAQIPADVMTSIEQEPVDATFAVSGGSVSVVESRDGFRFDAEAA
                     AEQVRELALGAGERSAELDGEVTEPELTTQEARSLGITEQVSTFTTNFPCCQSRVRNI
                     QRAAELVDGVVLEPGDTFSLNGHLGERTVAKGFTTGGAIYDGEFVEQVGGGVSQFTTT
                     MFNAAFFGGYAIPQYKAHSYYISRYPVGREATLNWPNVDLKVHNNSPHGILVKAATSG
                     TSVTVTFYGTKWVEVSEVTGGRTNVKPPPTKYKENPALAPGEERVVSQGREGFDITVT
                     RILKFPDGREERERFFTRYLPEPRTVERNTTSPSPSPSPEPTGTASPSPSPTASEPPP
                     Q"
     gene            3066..>3435
                     /locus_tag="VNU01_04550"
     CDS             3066..>3435
                     /locus_tag="VNU01_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31919.1"
                     /translation="MPVRRLRLGAVLVLVCALVASLAAPAGAASSEVRRLRQTKARIA
                     EVQREIGKVKAQAAQKEAALAAAQRQVNTILDAVASAEEAVARQEAAVEAARVRLEEL
                     RAEEERRRQVLRSRTLRLYKQ"
BASE COUNT          442 a         1265 c         1278 g          450 t
ORIGIN      
        1 gagcgtgcgc ggcgcatctc ggccaccacc gcggacagcg ccgcggattc cgggacggcc
       61 agcgcccggc tcatcagcgt gcgcaccggg acgccgtcgc ggtcgccggg cgcgtggcgc
      121 agcaggtcct tggcctgcac cacgcccagg atctcgtcgc ggtcggcgtc gacgacgggg
      181 aagcgcgagt ggccgctgct ggccgcgacg gcgcgcaggt cggcgccggt ggcgtccgcc
      241 ggcacgctgc tcacctgcgg gcgcggcacc atcacgtcgg cggcgcgcaa ctggcggaac
      301 tccagggccc gcgtcagaag ctgcgcctgg tccgcggtga ggtgcccgcc ggaggaggac
      361 tcgacgatga tgagctcgag ctcctccggg gacacgccgg tctccaggtg ctccaccggc
      421 tcgatgccca gcgcccgcag cagggcgttc gccgagctgt cgaagatgcg gatcagccag
      481 cccgccaggc gcgtgtacag cagcgtcggc gcggccagcc gactcgcgaa cgtctccggg
      541 cgcgcgatcg cgaggttctt gggcgccagc tcgcccacca ccatctgcag catggtcgcc
      601 agcacgagcg ccaccgtggc ggccaggccg ccgcgcgcgc cctcggggac gcgcgccagc
      661 gtgagcgccg gcgcgagcag ctcggcgatg atcggcgtgg cgatgaagcc gagcaccagc
      721 gacgtcgcgg tgatgccgag ctgcgcgccg gacagcacga acgacaggcg ctgcaccacg
      781 cgcacggccc gggcggcgcg cgggtcaccc tcggcggccc gctcctccag gtggctgcgg
      841 cgcaccgcga cgaaggcgaa ctccgccgcg acgaagtacg cgttgcccag gatgagggcg
      901 aggatcgcga gaaggccgaa ggggctggtc attccccgcg caggctactg cggcggcggc
      961 tcggacgccg tgggcgacgg cgacgggctg gcggtgcccg tcggctccgg cgacggcgac
     1021 gggctggggc tggtggtgtt gcgctcgacg gtgcgcggct ccggcaggta gcgggtgaag
     1081 aagcgctcgc gctcctcgcg gccgtcgggg aacttcagga tgcgggtgac cgtgatgtcg
     1141 aagccctccc gcccctggct caccacgcgc tcctcgccgg gtgccagcgc cgggttctcc
     1201 ttgtatttcg tgggcggcgg cttcacgttg gtgcggcccc cggtgacctc gctcacctcg
     1261 acccactttg tgccgtagaa ggtcacggtc accgacgtgc cgctggtcgc cgccttgacc
     1321 aggatcccgt gcggggagtt gttgtggacc ttgaggtcca cgttcggcca gttcagcgtc
     1381 gcctcgcgcc ccaccgggta gcggctgatg tagtagctgt gcgccttgta ctgcgggatc
     1441 gcgtagccgc cgaagaacgc cgcgttgaac atcgtggtgg tgaactggct gacgccgccg
     1501 cccacctgct cgacgaactc gccgtcgtag atcgccccgc ccgtcgtgaa gcccttggcc
     1561 acggtgcgct cgcccaggtg gccgttcagc gagaacgtgt cgccgggctc cagcacgacg
     1621 ccgtcgacaa gctccgccgc gcgctggatg ttgcgcaccc ggctctggca gcacgggaag
     1681 ttggtggtga acgtggacac ctgctcggtg atgccgaggc tccgcgcctc ctgggtggtg
     1741 agctccggct cggtcacctc gccgtccagc tcggcgctgc gctcgccggc gcccagcgcc
     1801 agctcccgca cctgctccgc cgcggcctcc gcgtcgaagc ggaagccgtc cctggactcc
     1861 accacggaca ccgagccgcc cgagacggcg aacgtggcgt ccaccggctc ctgctcgatc
     1921 gacgtcatca cgtcggccgg gatctgcgcc tccagggcct ccggcgagac ccgcagctcc
     1981 agggacacct cgtcgtcgtg cgtcacgcgt tccacggtca gcaccgcgcc gaggtcctcc
     2041 ggcgtgagcg tcagcgtcac gtcgccgcgg cgcagctcca ccgacgcggc cagcgcgcgc
     2101 cgcgccgcct cctcgacctc gtccacgtcg gcggacgtgg tcagcggctc cagcggcgcg
     2161 gtgcgcgcgc gcacctccga gcggccgtgg aacagcgcgt ccaggacgtc ctcccgcagg
     2221 gcgtcgcggc gcacgtccgc gcccggcttg ggctccacgc gcgcgaccga ggcgccctcg
     2281 aacgccagcg agccctcgat ggggtccacg cgcagccggc gctgcgcctg ctccagccag
     2341 tcgctcagcc gctcctggtc cacctcctgg accgcctcga ccgtcgtggt gccgaagaag
     2401 gcgcgcacct ggtcggcgaa cgccgcgaac gggttgagct ggcgcccgcg gcgcagcacc
     2461 gccttcacgg tgccgtcgac gtccagcgag tagcccagtt ccgcgccggt cgcggccacc
     2521 gtcgcctcct cggtcgcgcc cgccgacggg acgggcgcgc gcaccacggt caccgactgg
     2581 gcggtgcgct cgcgcgcgag gtcgctgacc aggtcgtgca ggcgcgcgcc gttcatgccg
     2641 gagacgtcct gaccgtccag gtgcacgccc ggcagcgcgc cggcgcgggc gacgcgcagg
     2701 ccgaacgtga gcagcagcat cacgccgaag gcgatcccga cgacgcgcag cgcgggcacg
     2761 gcgtagcccc gcgcgcgcgc aagcgccgcg gagcggaagg agggttgcgg ggtgtccacc
     2821 atggggcggg gccagggtag cagcgggttt tagccgtgcc ggggtggcgc gacgccgcat
     2881 ccaggggctt tgcgcgggac ccgcgcgggc accctggccg ttcgggcagg tgcgggtttt
     2941 gaagtgcctg cccgtgttgc ttagcctgat gacggccatt cgccagggac cccttccacc
     3001 gcaggtgttc ctggcgcgcc cctgaagccg cgccccgcag cgggcgcgca gacgtatgga
     3061 gagtcatgcc ggtacgccgt cttcgcctgg gcgcagtgct ggtgctggtg tgcgcgctgg
     3121 tcgcgtcgct cgccgcgccg gccggtgcgg cctccagcga ggtccggcgc ctgcggcaga
     3181 cgaaggcgcg catcgccgag gtgcagcgcg agatcggcaa ggtcaaggcg caggcggcgc
     3241 agaaggaagc ggcgctggcc gccgcgcagc gccaggtgaa caccatcctg gacgcggtcg
     3301 cctccgcgga ggaggccgtc gcccgccagg aggccgcggt cgaggcggcg cgggtgcgcc
     3361 tggaggagct ccgcgcggaa gaggagcgcc gccgccaggt cctgcgcagc cgcacgctgc
     3421 gcctgtacaa gcagg
//
LOCUS       DATMKC010000153         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_342673, whole genome shotgun sequence.
ACCESSION   DATMKC010000153 DATMKC010000000
VERSION     DATMKC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342673"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..834)
                     /locus_tag="VNU01_04555"
     CDS             complement(118..834)
                     /locus_tag="VNU01_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH31920.1"
                     /translation="MSPIQGRRGKVVIVDDEPDVLESTAMIVRALGYTPVTVDDPSLV
                     VDVVEREQPGLLLQDLRMPGLNLAGLVAALRLNPKTADVPLVFFSAHADVAATASRHD
                     AWGYLAKPFSPQELERLLQRVFATHEEAPAAQALPKDLSRDVRAMFHEQWNLIQALNN
                     YVSVLRAARGGDQALRAIHGIEEVLLKLQSRTDHLQGYLLGVAASLEPAVAPRPEAKA
                     SGAEARRASTRAAGPGQLPA"
     gene            1579..>3128
                     /locus_tag="VNU01_04560"
     CDS             1579..>3128
                     /locus_tag="VNU01_04560"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWH31921.1"
                     /translation="MLAQETDEEPAPAAAGVPMRFGMDANSVSAQAAAGVKPDYGTMW
                     LGPWTLTSGWGGPNSQLTSMRNAGVTPAIHFYYWGDDISQSCLKNGCYSSLHSAQKDQ
                     AGWQLLAEQMVDNLNARMGGEPVVIFMETEFNKADVQTWEPLDAMLEEKARFIKQGYP
                     NAQIVLAIGNWNSPAWKTWDRAAAASDYVGIQGMRGSTRDSLSHYLNLYEASLSGART
                     LQGLFGKPVFLTDIALSSYPEPEYLGHQQSELKQFFDNLPALKDAGVEAMLYRGWRDN
                     PTMNLANYYGQAERHWGLNYAYSTGAMKPSGHVWVAGVKDERAAMANVAPVARFQAAM
                     TDMTVEVDARTSSDANGDALTYSWRFGDGSTATGATARRTYEAPGSYTVTLTVHDGRA
                     QATTSRTVQAVLPNRPPAASFTATTTDLAVALDARASSDADGDALHFAWELGDGRTAT
                     GATVSHTYGTAGSYVVTLTVTDDRGASTTARTLVTVAPARVNQAPTAAFTATGRDLDV
                     AVDGTASD"
BASE COUNT          476 a         1164 c         1066 g          422 t
ORIGIN      
        1 gccgcgggcg aggcccgctg tgccgccgct gcgccccttg cctcgcgcgg ggtcgcggcc
       61 ctagcgtgca gcggcggcac agcgggcctc gcccgcggcg gcgcaggctc cttcggtcta
      121 tgccggaagc tgtccagggc cggctgcgcg cgtcgacgcg cgccgtgcct ccgcaccaga
      181 agccttcgcc tccgggcgcg gggcgacggc gggctccagc gatgcggcca cgcccagcag
      241 gtagccttgc aggtggtccg tgcgcgactg cagcttgagc aggacctcct cgatgccgtg
      301 gatggcgcgc agggcctggt cgccgccgcg cgccgcgcgc agcacgctca cgtagttgtt
      361 gagcgcctgg atgaggttcc actgctcgtg gaacatggcg cgcacgtcgc gcgagaggtc
      421 cttcggcagc gcctgcgcgg ccggcgcctc ctcgtgggtg gcgaacacgc gctgcagcag
      481 gcgctcgagc tcctgcgggc tgaacggctt ggcgaggtag ccccaagcgt catgccggct
      541 ggccgtggcg gccacgtcgg cgtgggcgct gaagaacacg agcggcacgt cggcggtctt
      601 cgggttcagg cggagcgccg cgacgaggcc ggccaggttg aggcccggca tgcgcaggtc
      661 ctgcagcagg agcccgggct gctcgcgttc gacgacgtcg acgacaagcg aggggtcgtc
      721 caccgtcacc ggcgtgtagc cgagggcgcg gacgatcatc gcggtcgact ccagcacgtc
      781 gggctcgtcg tcgacgatga cgaccttccc gcggcgtccc tggatcgggc tcacatgcag
      841 gcgttggaga gggagctaaa gacgcgtgtt ggaaaccggc gccaggaaac ggggagaagt
      901 gcccgcatct ggcaaggaaa ccgaggattc ctcctgttgg accgccgccg ggccgcggac
      961 gcggggtgtt gtccgcgcgc tggccggggc gtggtgcttc ctgccaccgc gcgtgacgag
     1021 gcgccaacat gctgtcctac ccgctgctcc ctcgccacgc cgcccggagc ggccggccgg
     1081 gtccctgccg ctgcgcacgc gggatgaccg caggcccgtc tgcctccgcc gctcgcgcct
     1141 ggacgcgccc acctcgccct gcgacgtcgg cccttgcctc gcgcgtgcgc aaggtgactg
     1201 gcgccagccg gtcgtgctct cctcccacgc cgcccgtcct gccgcgcgtg ggccggcctg
     1261 cgacttgcgg cctgccctcg cgcgagcgag cggccgatgc tcggcagggc gaaaggtcct
     1321 gcgcgcaggg cgggaacgtg tccaagatac ggctaaatcc gagattctgg ggaaagcctc
     1381 cggtatagaa ctcctcggtt ccgactaaat cggtcgagtc acggagcgag ggttggagct
     1441 gccgcagcct ccgcggcaaa ggcggctctt gcggcgcgga aaacgcgccc gaaaccaacc
     1501 caacacgtga gttccatgac acgacttcgg accagtgctc tctgccttct cttcctgaca
     1561 gccgccaccg tacctttcgt gctggcgcag gagacggacg aagagcctgc cccggccgcg
     1621 gccggcgtgc cgatgcgctt cggcatggac gccaactccg tctccgccca ggcagcagcc
     1681 ggcgtgaagc ccgactacgg caccatgtgg ctcggcccct ggacactgac ctcgggatgg
     1741 ggcggaccca acagccagct cacctcgatg cgcaacgccg gcgtcacccc ggcgatccac
     1801 ttctactact ggggcgacga catctcgcag tcctgcctga agaacggctg ctactcgtcg
     1861 ctgcacagcg cgcagaagga ccaggcgggc tggcagctgc ttgccgagca gatggtggac
     1921 aacctgaacg cccgcatggg cggcgagccg gtcgtcatct tcatggagac cgagttcaac
     1981 aaggccgacg tccagacctg ggagccgctt gacgcgatgc tcgaggagaa ggcccgcttc
     2041 atcaagcagg gctaccccaa cgcgcagatc gtcctggcca tcggcaactg gaacagcccc
     2101 gcctggaaga cctgggaccg cgcggccgcc gcaagcgact acgtgggcat ccagggcatg
     2161 cgcggcagca cccgcgactc gctctcgcac tacctcaacc tgtacgaggc gagcctctcc
     2221 ggcgcccgca ccctgcaggg cctgttcggc aagcccgtct tcctgacgga catcgccctc
     2281 agctcctacc cggagcccga gtacctcggc caccagcagt ccgagctgaa gcagttcttc
     2341 gacaacctcc cggcgctcaa ggacgccggc gtggaggcca tgctgtaccg cgggtggcgc
     2401 gacaacccca ccatgaacct ggccaactac tacggccagg cggagcgcca ctggggcctc
     2461 aactacgcct acagcacggg cgccatgaag ccctccgggc atgtctgggt ggccggcgtc
     2521 aaggatgagc gcgccgcgat ggcgaacgtc gccccggtcg cgcgcttcca ggccgccatg
     2581 acggacatga ccgtcgaggt ggacgcgcgc accagcagcg acgccaacgg cgacgccctg
     2641 acgtactcct ggcggttcgg cgacggctcc accgcgaccg gcgccaccgc ccgccgcacg
     2701 tacgaggcgc ccggcagcta cacggtgacg ctcaccgtcc atgacggacg cgcgcaggcg
     2761 acgacctccc gcacggtgca ggcggtcctg ccgaaccggc ctcccgccgc ctccttcacc
     2821 gccaccacga cggacctcgc cgtcgccctg gacgcccgcg cgtcctcgga cgccgacggc
     2881 gacgcgctcc acttcgcgtg ggagctcggc gacggccgca cggccaccgg cgccaccgtg
     2941 tcgcacacct acggcaccgc cggctcctac gtggtcaccc tcacggtgac cgacgaccgg
     3001 ggcgcctcga ccacggcgag gacgctggtc accgtggccc cggcccgggt gaaccaggct
     3061 ccgaccgcgg ccttcaccgc caccgggcgc gacctggacg tggccgtcga cggcaccgcc
     3121 agcgatga
//
LOCUS       DATMKC010000154         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_123936, whole genome shotgun sequence.
ACCESSION   DATMKC010000154 DATMKC010000000
VERSION     DATMKC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123936"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VNU01_04565"
     CDS             <1..447
                     /locus_tag="VNU01_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31922.1"
                     /translation="VMGMIVTTVLLMGATFLLRARWQLPRHTLLFLFTGFALLMSGLH
                     GFREVALVLPAALAGALADALDGQRASSRTIGVAVPTVLWLAWFATYAALWGLDWTPE
                     LWLGAVVFAALAGLGLSYLTDPPRALRALAPEAQPQEAEAPVPVAR"
     gene            510..>2153
                     /locus_tag="VNU01_04570"
     CDS             510..>2153
                     /locus_tag="VNU01_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWH31923.1"
                     /translation="MTATGELERGRQCFQRRAWADAYAALSAAAARSPLDPEDLERLA
                     TAARLVGQDAACADALERAHHAFLAGGDGPAAARCAFWLGFGLLQQGDTARGSGWLAR
                     AERVLADGPPGTVLHAYLGIPAALAAVGRGEFADAHDRFAAVAEAGERHGDSGLVMLA
                     RHGQGRALIRMGEIERGAALLDEAMVGVTTSPDISPVFAGIVYCSVIEACHEIFDLRR
                     AGEWTDALTRWCAAQPGLVPYRGQCLIHRAQILQFHGAWADAAGEADAACDRLADPPG
                     QPALGLAYYQRAELHRLRGEARPAEEAYHQAAVHGRDPQPGLALLRLAEGNVEAARAA
                     VQRALDETRERGARLRLLAARVEIALPHDQEAARAACEELAAIAEVFPAPYARAVSAH
                     AAGALRLADGDAAGALSLLRDARAVWQELDAPYDAARAGVLIGQACWRLGDHDGAATH
                     WDAAEAVFRELGAMPDLAGLPRPDRQAREQAPRGLTRRELEVLRLVAAGRTNREIAEA
                     LVISEHTVARHVQNMFTKLGVTSRTAAAATSRSTSSSRRV"
BASE COUNT          226 a          834 c          838 g          255 t
ORIGIN      
        1 gtcatgggca tgatcgtcac caccgtgctg ctgatgggcg ccacgttcct gctgcgcgcg
       61 cgctggcagc tcccgcgcca cacgctgctg ttcctgttca cgggcttcgc cctgctgatg
      121 tccgggctgc acggcttccg cgaggtggcg cttgtgctgc ccgccgcgct cgccggcgcg
      181 ctcgccgacg ccctcgacgg gcagcgcgcg tcgtcgcgca ccatcggcgt ggcggtgccg
      241 acggtgctct ggctggcgtg gttcgccacg tacgcggcgc tgtggggcct ggactggacg
      301 ccggagctgt ggctgggcgc ggtggtgttc gcggcgttgg ccgggctggg cctgagctac
      361 ctcaccgacc cgccgcgcgc cctgcgcgcg ctggcgccgg aggcgcagcc gcaggaggcg
      421 gaagcgccgg tgcccgtggc gcgctgaccc tcgacgccgc ttgccgtccc ccgcgatact
      481 cggacgccag cggtgcggca gggggtggca tgacggcgac cggcgaactg gaacgcggac
      541 ggcagtgctt ccagcggcgc gcgtgggccg acgcctatgc cgcgctgagc gccgcggccg
      601 cccgctcccc gctggacccg gaggacctgg agcgcctggc cacggccgcg cgcctggtgg
      661 gccaggacgc cgcgtgcgcg gacgccctgg agcgggcaca tcacgccttc ctcgccggcg
      721 gcgacgggcc ggcggcggcg cgctgcgcct tctggctggg cttcggcctg ctgcagcagg
      781 gtgacaccgc gcggggcagc ggctggctcg cccgcgccga gcgcgtcctg gccgacggcc
      841 cccccggcac cgtgctgcac gcctacctgg gcatcccggc cgcgctggcc gcggtggggc
      901 gcggcgagtt cgccgacgcc cacgaccgct tcgccgccgt cgccgaggca ggcgaacgcc
      961 acggcgacag cggcctggtg atgctcgcgc gccacggcca gggccgcgcg ctcatccgca
     1021 tgggcgagat cgagcgcggc gccgcgctgc tggacgaggc gatggtcggc gtcaccacca
     1081 gcccggacat ctccccggtg ttcgccggca tcgtctactg cagcgtcatc gaggcctgcc
     1141 acgagatctt cgacctgcgc cgcgccggcg agtggaccga cgcgctcacc cggtggtgcg
     1201 ccgcccagcc cggcctggtc ccctatcgcg ggcagtgcct gatccaccgg gcgcagatcc
     1261 tgcagttcca cggcgcgtgg gccgacgccg ccggggaggc cgacgccgcc tgcgaccgcc
     1321 tggccgaccc gccgggccag ccggcgctgg gcctggcgta ctaccagcgc gccgagctgc
     1381 accggctgcg cggcgaggcg cgcccggccg aggaggccta ccaccaggcc gccgtgcacg
     1441 gccgcgaccc gcagcccggg ctggcgctgc tgcggctggc cgagggcaac gtggaggcgg
     1501 cgcgcgccgc cgtccagcgc gcgctggacg agacccggga gcgcggcgcg cgcctgcgcc
     1561 tgctggccgc ccgcgtggag atcgcgctgc cgcacgacca ggaggcggcg cgcgccgcct
     1621 gcgaggagct cgccgccatc gccgaggttt tccccgcgcc gtacgcgcgt gccgtctccg
     1681 cccacgccgc cggggccctg cggctggccg atggggacgc ggcgggtgcg ctgagcctgc
     1741 tgcgcgacgc ccgcgcggtg tggcaggagc tggacgcgcc ctacgacgcg gcacgcgcgg
     1801 gcgtgctgat cggccaggcg tgctggaggc tgggcgacca cgacggcgcc gcgacgcact
     1861 gggacgccgc cgaggcggtg ttccgcgagc tgggcgccat gccggacctg gcggggctgc
     1921 cacggccgga ccgccaggcg cgcgagcagg cgccccgcgg cctgacccgc cgcgagctgg
     1981 aggtgctgcg gctggtcgcc gccgggcgca ccaaccgcga gatcgccgag gcgctggtca
     2041 tcagcgagca cacggtggcg cgccacgtcc agaacatgtt caccaagctg ggcgtcacgt
     2101 cgcgtaccgc cgcggcggcg accagccgca gcacctccag ctcgcggcgg gtc
//
LOCUS       DATMKC010000155         3901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_266991, whole genome shotgun sequence.
ACCESSION   DATMKC010000155 DATMKC010000000
VERSION     DATMKC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266991"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VNU01_04575"
     CDS             complement(<1..1087)
                     /locus_tag="VNU01_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31924.1"
                     /translation="MALLLGALLLAAAPAQAQTRTYAATATGTSAQVLVGGQQLQLGH
                     TSVAIRSDAAQEVPNCPADQVACAHAATVVGVNDALASAPKGPGEAVGEPNGALPEDT
                     APLAIAHLGRAVAKAATGGASGETDLLHLELTATQTIAENAAPVVDGLKQVSEQLFGP
                     LAEGDPSGELGQRLKQSFDSLVENIDENPLVEIDLLPSLSTVTDNGTLTTAEAIAKGA
                     QIVISPTAASDVVAPEGLIIVEIGSSRAYADSAGKTEGNAAAVLLKIFNPVTGAYDEH
                     GVPAGEYTCAGEGTPLYTCVGLGTTKAEAGAFQAGGVSVNALDGNLKIEVGAAQAGAS
                     ASVAPAAGPPAPAPLPTTGGGLAHRGST"
     gene            complement(1373..1747)
                     /locus_tag="VNU01_04580"
     CDS             complement(1373..1747)
                     /locus_tag="VNU01_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31925.1"
                     /translation="MALVMSSAAGRTRLHAEGDLRVLWLTAGRSELALSVQGLWLRRG
                     RRETELRWEEIQQVQAVAAPGLRRGSVRVEVFSPDGGVHALGPFGRAPAERWVAACAQ
                     AATEAGLEPMPLEGATGFALHA"
     gene            complement(1766..3202)
                     /locus_tag="VNU01_04585"
     CDS             complement(1766..3202)
                     /locus_tag="VNU01_04585"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HWH31926.1"
                     /translation="MPDRRRPADDGRRSGEAPGVQAGRLRRGANIAKLSASTGAGAMG
                     AWMRGLTGDDAAEQRFHAETAQRLLEVLGGMKGAAMKLGQLASFVDLDLPPEIQAVYH
                     DVLAELRAAAPPLDPERIAEVVGEQFGAPPEEVFARWERTPLASASIGQVHAATLPDG
                     TEVAVKVQYPGVAEAVEADLANAETFAPMARLAMPNLQVKPLLEELRLRIADELDYQR
                     EAAYQHAFAERYDGHPFVRVPAVLADWCRPRVLVAELMRGLDFEAFLAQADAAQRRRA
                     AEIIFRFTFGSLHRFRLFHGDPHPGNYLFPLDGAGRWDGTVTFLDFGSVKPFTSAARD
                     RIHRQVRAVIDGDAAELVAALTDAGFVREGQDVDPERLLAWFRLFNAPVVADEPFTYT
                     PEFAREVNRTTSDPRAGWFDLLRKLNMPPDYLLLNRIQLGVNSILGRLGATANWHRIS
                     QELMGDAPAATPLGEAEAAFLASSPFRA"
     gene            3248..>3901
                     /locus_tag="VNU01_04590"
     CDS             3248..>3901
                     /locus_tag="VNU01_04590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWH31927.1"
                     /translation="MRVRSRYRILDLVQSLDPERDHERIVRLSGLWDFPWDGARALEL
                     ALFRTFAVPSIGGLLYRTGEFTKRAQKRYDDTELLIAEFIEHGWSSERGARAIARMNA
                     IHSRYRIGNDDFLYVLSTFVFEPSRWNARFGWRPLSAVERRAGYVFWREVGTRMGIAG
                     IPETAAELEAFNVAYEREHFRYSAKNAAVARATIELFCSWYLPRPLWPLGERAVCALL
                     "
BASE COUNT          513 a         1393 c         1430 g          565 t
ORIGIN      
        1 gtgtggatcc ccggtgagcc aggccaccac cggtggtcgg cagcggtgcg ggagccggcg
       61 gcccggcggc cggggccacc gaggcgctgg cgccggcctg ggccgcgccg acctcgatct
      121 tcaggttgcc gtccagcgcg ttgacgctca cgccaccggc ctggaaggcg ccggcctcgg
      181 ccttggtcgt gcccaacccc acgcaggtgt acagcggggt gccctcgccg gcgcaggtgt
      241 actcgccggc ggggaccccg tgttcgtcgt aggcgcccgt caccggattg aagatcttca
      301 gtaggacggc ggccgcgttg ccttcggtct tgccggcgct gtccgcgtag gcgcggctgg
      361 agccgatctc cacgatgatc aggccctcgg gagccacgac gtcgctcgcg gcggtggggc
      421 tgatgacgat ctgcgcgccc ttggcgatgg cctcggcggt ggtgagcgtg ccgttgtcgg
      481 tcacggtgct gagcgacggc aggagatcaa tctccaccag cgggttctcg tcgatgttct
      541 cgaccaggga gtcgaacgac tgcttcaggc gctggccgag ctcgccgctc gggtcgccct
      601 cggccagcgg gccgaacagc tgctcggaga cctgcttgag gccgtccacg accggcgccg
      661 cgttctccgc gatcgtctgc gtggccgtga gctcgaggtg gagcaggtcg gtctcgccgg
      721 aggcgccgcc ggtcgccgcc ttcgccacgg ccctgcccaa gtgggcgatc gcgagcggag
      781 ccgtgtcctc gggcagcgcg ccgttcggct cgccgacggc ctcgccgggg cccttggggg
      841 cggaggccaa ggcgtcgttg acgccgacga ccgtggccgc gtgggcacac gcgacctggt
      901 cggccgggca gttgggaacc tcctgcgcgg cgtcggaacg gatcgcgacg gaggtgtgac
      961 cgagctgcag ttgctggccg ccgacgagga cctgggccga cgtcccggtc gcggtggcgg
     1021 cgtaggtgcg cgtctgcgct tgggccggag ccgccgccag caagagtgcg cccaggagaa
     1081 gcgccacgga gcggatcttc atatcgatgt accccccagg gagcggatgc ccccggcagc
     1141 tcgtcgtctt cgggtttggt acggatcgcc gcaatctttg attacggccg gttgcgctcg
     1201 ggatgggtgt ccgttgcgcg gatctacctc tcgtaacgac cctccggccg gggggttacg
     1261 cccgtcgagg caatcaatcg ctgaaagata ccgggaatga ggcccttttg cgaaagggac
     1321 atggcccgtt cggacagggt cggctggctg gtcacgcagc gcaaccttcc catcacgcgt
     1381 ggagggcgaa acctgtggcg ccctcaagcg gcatcggctc cagccccgcc tcggtcgccg
     1441 cctgggcgca ggcggcgacc cagcgctccg ccggcgcgcg gccgaatggt cccaacgcat
     1501 ggacgccccc gtctggagag aacacctcga cccgcacgct gccgcgccgc agtcccggcg
     1561 ccgccaccgc ttgcacctgc tggatctcct cccagcgcaa ctccgtctca cgccgtccgc
     1621 gccgcagcca caggccctgc acggacaggg ccagctcgga gcggccggcg gtcagccaca
     1681 gcacccgcag gtcgccctcg gcgtgcagcc gggtgcggcc ggcggcggaa ctcatcacca
     1741 gcgccatgcg cgcaagacta cgggatcagg cccggaacgg tgatgaggcg agaaacgcgg
     1801 cctccgcctc gccgagcggc gtcgccgccg gggcgtcgcc catcagctcc tgggagatgc
     1861 ggtgccagtt cgccgtggcc cccagtcgcc ccaggatgga attcacaccc agctggatgc
     1921 ggttcagcag cagatagtcc ggcggcatgt tgagcttgcg caacaggtcg aaccagccgg
     1981 cgcgagggtc cgacgtcgtg cggttgacct cgcgggcgaa ctccggcgtg taggtgaacg
     2041 gctcgtcggc caccacgggg gcgttgaaca gccggaacca ggccagcagg cgctccgggt
     2101 ccacgtcctg gccctcgcgg acgaagccgg cgtcggtcag cgcggcgacg agttccgcgg
     2161 cgtcgccgtc tatcaccgcc cgcacctgcc ggtggatgcg gtcccgcgcg gcgctggtga
     2221 acggcttgac gctgccgaag tccaggaacg tcaccgtgcc gtcccagcgg ccggcgccgt
     2281 ccagcgggaa caggtagttg ccggggtgcg ggtcgccgtg gaacagccgg aagcggtgca
     2341 gcgagccgaa cgtgaagcgg aagatgatct cggcggcccg ccggcgctgt gccgcgtcgg
     2401 cctgcgccag gaacgcctcg aagtccaggc cgcgcatcag ctcggcgacc agcacgcggg
     2461 gccggcacca gtccgccagc acggccggca cgcgcacgaa cgggtggccg tcgtagcgtt
     2521 cggcgaaggc gtgctggtag gcggcctccc gctggtagtc cagctcgtcg gcgatgcgca
     2581 gccgcagttc ctccagcagc ggcttgacct ggaggttcgg cattgccagc cgcgccatgg
     2641 gcgcgaacgt ctccgcgttc gcgaggtcgg cctccaccgc ctcggcgaca ccggggtact
     2701 gcaccttgac cgcgacctcg gtgccgtccg ggagggtggc ggcgtggacc tggccgatgc
     2761 tggcgctggc cagcggcgtg cgctcccagc gcgcgaacac ctcctccggc ggcgcgccga
     2821 actgctcgcc gaccacctcg gcgatgcgct cggggtccag cggcggcgcc gcggcgcgca
     2881 gttcggccag gacgtcgtgg tacacggcct ggatctccgg cggcaggtcc aggtccacga
     2941 agctggcgag ctggccgagc ttcatggcgg cgcccttcat gccgccgagc acctccagca
     3001 gccgctgcgc ggtctcggcg tggaagcgct gttccgccgc gtcgtcgccc gtgagcccgc
     3061 gcatccaggc gcccatcgcg cccgcccccg tggacgccga cagcttggcg atgttggccc
     3121 cgcgacgcag ccggccggcc tggacgcccg gcgcctcgcc gctgcggcgg ccgtcgtctg
     3181 cggggcggcg tcggtcgggc atggccccag tatgctgcgc gggtcggacc cagggagagc
     3241 ggaggccgtg agggtgcgca gccggtaccg gatcctcgac ctggtccagt cgctggatcc
     3301 cgagcgcgac cacgagcgca tcgtgcgcct gtccgggctg tgggacttcc cgtgggacgg
     3361 cgcgcgcgcc ctggagctgg cgctgttccg cacgttcgcc gtgcccagca tcggcggcct
     3421 gctgtaccgc acgggcgagt tcaccaagcg ggcgcagaag cgctacgacg acaccgagct
     3481 gctgatcgcc gagttcatcg agcacggctg gtcctccgag cgcggcgcgc gcgcgatcgc
     3541 gcgcatgaac gccatccact cccgctaccg catcggcaac gacgacttcc tgtacgtgct
     3601 gtccacgttc gtgttcgagc cgtcccgctg gaacgcgcgc ttcggctggc ggccgctgag
     3661 cgcggtggag cggcgcgccg gctacgtgtt ctggcgcgag gtcggcacgc gcatgggcat
     3721 cgccggcatc cccgagacgg cggcggagct ggaggccttc aacgtcgcct acgagcgcga
     3781 gcacttccgc tactcggcca agaacgccgc cgtcgcgcgg gccaccatcg agctgttctg
     3841 ctcctggtac ctgccgcggc cgctgtggcc gctgggagag cgggcggtgt gcgccctgct
     3901 g
//
LOCUS       DATMKC010000156        17136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_146074, whole genome shotgun sequence.
ACCESSION   DATMKC010000156 DATMKC010000000
VERSION     DATMKC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146074"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VNU01_04595"
     CDS             <1..1084
                     /locus_tag="VNU01_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH31928.1"
                     /translation="PSRRPGLRELGRAVTATLLSDTELERDRRLVEAHQAGDVSAFGQ
                     LYRLHHERLLRFCQRKTRDPHLAEEIAQEAFLKAYRALDGLNGGKRFYPWLTVIASRL
                     AIDHHRRAGRVQPSAAIELGAVDDTHERMERQVDREQVLQALERVRLRHREVLRLRDY
                     EDLSYDAIAQRLGTSPAVVQSLLHRARQALRREYLAVTEHAGLVPLLGPACAALRRLR
                     ARAGRAASWLPDPSALGAPLAAATLALGGALGAWTAPAPQGAASSGSAAAAVEPATGP
                     AAARLVAQPAPAVGEPAGVPPSRQGRGPRAPAEVWVGPAGAAEARAHGEDMPARVDTG
                     RAIVAADPETILEDTLHAAAGLVKGH"
     gene            1086..1268
                     /locus_tag="VNU01_04600"
     CDS             1086..1268
                     /locus_tag="VNU01_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31929.1"
                     /translation="MSIRRDLAVALLLLLLAALGAVPARAALEAPAAAAGRWGCVALD
                     GVAGFCVSSPVESSGL"
     gene            1546..2616
                     /locus_tag="VNU01_04605"
     CDS             1546..2616
                     /locus_tag="VNU01_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWH31930.1"
                     /translation="MSALLEAIEDERDRGLVERHQAGDDAAFSELYALHYGRLVRFCK
                     RLVRDSHVAEELAQDAFLRAYTSMDRLAGERRFYPWLTVIARRLVIDHVRRDGRVQPH
                     ANVEPVPAGATEDVVVQRLDGDHLLTALDRVRDRHRQVLHLRDWEGLSYEAIADRLGL
                     APTAVPPLLHRARLALRREYMLVTEGRVAAFLPLSAVTLAVRRLRDRVALWTSWLPDA
                     GAISAPMAGAVLAVGALLTVSPGAADAAGHRDAGTAAAVEDGHAAGSPAQLGEAPRDT
                     THGLGGRLERVPPPAPGGEPREVLVPDVAHVTLDPRPDADGSRPHDKEQPIYLNFGPV
                     GYAGDPERTAGHFKGVAEDRLP"
     gene            2637..2771
                     /locus_tag="VNU01_04610"
     CDS             2637..2771
                     /locus_tag="VNU01_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31931.1"
                     /translation="MRALKAAVLGLLAATVLLAGLAGPAAAEIDCVWIAGNRVCVPPE
                     "
     gene            2905..4059
                     /locus_tag="VNU01_04615"
     CDS             2905..4059
                     /locus_tag="VNU01_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWH31932.1"
                     /translation="MWEGTGEMAAVLNGSDLELDRDLVERHQAGDDAAFTQLYALHYG
                     RLVRFCLRLTRDHHTAEEIAQEAFARAFATLDRFGGERRFYPWLTVIARRLVIDHARR
                     NERVETRAHVDAGFASPAEDVVVQRLDGRHLTFAMDRLTVRHQDVIRLRDAEGLSYEA
                     IAERLGVPVTTIPPLLHRARATLRREYVRADRGRAASWLHLSPVIAAARRLRDRLAVW
                     GSHLPDASMFAAPMAGAVLGATALLAPQVMMGEQGDVRFGASTFGLHVPERPGAGQAE
                     AAGSATQPAEHAGQDHPAGNSKDFVNPSYALRDAGVRPEETVLRYVMDSDPATSDERQ
                     DEVRRMPIYYEEEYTGIYLGVNPEAAVHCVQSSWSCTETTRERAGSALGH"
     gene            4101..4283
                     /locus_tag="VNU01_04620"
     CDS             4101..4283
                     /locus_tag="VNU01_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31933.1"
                     /translation="MSKKLIVVAALALALLQATPAQASACCIICVGEPPNAVCILPGC
                     DPSCTPLVALARDQDW"
     gene            4295..5209
                     /locus_tag="VNU01_04625"
     CDS             4295..5209
                     /locus_tag="VNU01_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH31934.1"
                     /translation="MAVPEDLLDIYFKELGGVELLTAADEQELAKRMEAGRAAQERLA
                     AERAGTVTLSERERVELRTAADAGGAAFDRFVSANLRLVVSEAAKVARRSKLDLGELI
                     QEGNLGLIRAVEKFDWRRGLKFSTYATWWIRQALQRGMAGKERTIRIPMGVHANLMKV
                     RAAEARLSNELNRPPCLDELSDATNLTEDQVRAALEADFAVASLDKPVSDDHDAADLG
                     ALVARASDAPADEVIDRMFVEGVFDVAARQLPQRSWYVLQRRYGLDGFEPATLQEVGR
                     DIGVSRETVRKIEQQALAHLRDAVQVAA"
     gene            5244..5918
                     /locus_tag="VNU01_04630"
     CDS             5244..5918
                     /locus_tag="VNU01_04630"
                     /inference="COORDINATES: protein motif:HMM:NF025199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HWH31935.1"
                     /translation="MARKKKEPGKPGTISQLRMAWTQSRALDSRLAPYTAAAALLGFA
                     VPFVILLLLGRPVLGAVAGVLVAALAALSLFGRRASAVQLTAIEGQPGAAAAVVQSLR
                     GIWRLTPAVAVTRSQDLVHRVVGRPGIVLVGEGAPARVAQLLAQEKRRVARVAKDVPV
                     HEVSVGTGKGQVSLGDLRMHMMRLPRAVKPRQIGELERKLSALGEQNIPIPKGPMPTR
                     PPKRMR"
     gene            complement(5915..6232)
                     /locus_tag="VNU01_04635"
     CDS             complement(5915..6232)
                     /locus_tag="VNU01_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWH31936.1"
                     /translation="MPAADARWTFLTNHALVLLCVAADREIRVRDLADQVGITERAAQ
                     RILSELVDAGYIDKRRVGRRNVYAIHPERPLRHVLQRQHTVGELLDLLKPLAGGDGSA
                     SAA"
     gene            complement(6312..6758)
                     /locus_tag="VNU01_04640"
     CDS             complement(6312..6758)
                     /locus_tag="VNU01_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWH31937.1"
                     /translation="MDAVIRAAALYLFLLVVFRVSGRRTLSQATTFDFVLLLIIGEAT
                     QQGLLGQDFSMTNAFIVIVTLMLMDIGMSLFQQRVPAVSRYVEGLPVVLLEHGEPIKD
                     RMDKARVSESDLLEKARSSQGLERLEQIKYAVLEKDGSISIIPSSS"
     gene            complement(6768..7256)
                     /locus_tag="VNU01_04645"
     CDS             complement(6768..7256)
                     /locus_tag="VNU01_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31938.1"
                     /translation="MDLQINQDAGFQWREWAAQRVGWTLMSLLVLAAVVGLLGPGPLS
                     WSEAASADESATVDYLRWARRGREAGLTIRVDASHVRDGQVTVDVPARYFQDMRLRGV
                     TPSPEQVQANGDLLRFVFAVPQAGARARLEFDLEADGMGPLRGEVRVGTSTVRFSQFI
                     WP"
     gene            complement(7325..8203)
                     /locus_tag="VNU01_04650"
     CDS             complement(7325..8203)
                     /locus_tag="VNU01_04650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH31939.1"
                     /translation="MTASFHVRPLRADEADDVYDVTTDAFAAAAERAHDDPPQADERM
                     LAAHRRRIAHSVAHDAGGCWAAERDGRLIGAAIALLREDLWILSLLMVRGEAQGQGVG
                     RELMRHALAYAGDRPDGIIAASLDPFAWRRYWAAGFRLLPAALAKGAVRRAAIPAVAG
                     VRPGRPEDYELCADLGRRMRGASHGPDLAFLAEGPDGGFWVSDGAGDRGFVMTFKGSP
                     ALLAADQPATAQRLLWTALAESDGDVATYFVTGEQGWAFDVFFRAGLLVRPGTAVFTR
                     GRLGAMHPYLPNGPFL"
     gene            complement(8200..8874)
                     /locus_tag="VNU01_04655"
     CDS             complement(8200..8874)
                     /locus_tag="VNU01_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31940.1"
                     /translation="MRRSIPAALVLLATLSMVACAGGTPPSPADGQQGQTQAAVPGEL
                     PAAPEDTTWYDDVEMAQAQVEADVDTVWLAGWFAPEALDGPTVADMEPRHRKAWGLSP
                     DPTREELARAAFAALEGEGPVDLLHPLEGVQLRLLSAGVEDRDGGPTAVLDFGAGISA
                     TNQLGSAGGQAAKAQFLAMVAHYFPDARQVVVTVEGRDAPALFHDEVWDAPVPLEPTA
                     GQEGAA"
     gene            complement(9120..9314)
                     /locus_tag="VNU01_04660"
     CDS             complement(9120..9314)
                     /locus_tag="VNU01_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HWH31941.1"
                     /translation="MKATTSVDEELLTPSEVAKLFRVDPKTVTRWAKSGKLSSIRTLG
                     GHRRYRASEVRALLEGQQLI"
     gene            9533..10609
                     /locus_tag="VNU01_04665"
     CDS             9533..10609
                     /locus_tag="VNU01_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HWH31942.1"
                     /translation="MDSVVFDHFDGHEQVVFAADPASGLRCIVAIHSTALGPGLGGTR
                     FKAYPTEEAALVDVLRLSRAMTYKNACAGLDHGGGKAVIIGDPAVLRSEALLRAYGRV
                     IASLGGRYITACDIGTKPADMSVIRHETRWVTGMDPADGGSGDSGVLTAWGVYAGIKA
                     CLQHVSGDRSLAGRHVAVQGVGKVGRRLAEHLAADGARLTLADVDEPAVRDLAARLNA
                     EVVPVEAVHRVDCDVFSPNALGAVLNDDTIPELSCRVVAGGANNQLAEERHAKLLHER
                     GVLYAPDFVINAGGVIQISDELHPAGHSPERAKTKTETIADRLLEIFAHAEAEGIDTE
                     QAANAVAEKRINAVGRLRSFWLSE"
     gene            complement(10723..11124)
                     /gene="trxA"
                     /locus_tag="VNU01_04670"
     CDS             complement(10723..11124)
                     /gene="trxA"
                     /locus_tag="VNU01_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWH31943.1"
                     /translation="MATVELTADTFQQTVLDAEGLVLVDFWAEWCGPCRSFAPVFERV
                     SERHPDAVFGKVDTEAEQELAAAFQIMSIPTLMLFRDQVLLYSQPGSLPEEALEDLIQ
                     QAKALDMDDVRRQIAEHESQHAEEGSHQHAE"
     gene            complement(11315..12331)
                     /gene="purM"
                     /locus_tag="VNU01_04675"
     CDS             complement(11315..12331)
                     /gene="purM"
                     /locus_tag="VNU01_04675"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWH31944.1"
                     /translation="MSDGLTYSDAGVSLEAADASVELLKEHVARTHRAGVLGGIGGFG
                     GLFELDLGRWKHPVLVSGTDGVGTKIELARQLGRHDTVGVDLVAMVVDDIVVTGAEPL
                     FFLDYVAVGKLDPAHLAEIVAGIAQGCVEAGCALVGGETAEHPGVMPAGQYDLAGFGV
                     GVVERDRMLGPERVQAGDQIVAMASSGPHSNGYSLIRRIVDGKDLHGEHGLGMPLGEA
                     LLRPTRIYTKDCLALAASGNVHALCHVTGGGIPGNLPRVLPEGLGATVDTSTFEIPAV
                     FRKLQEWGRVAEDECWRVWNMGAGMLAVVPDGRAAVDLLAQRGVDAWVCGAVTDTPGV
                     RLSV"
     gene            complement(12328..13800)
                     /gene="purF"
                     /locus_tag="VNU01_04680"
     CDS             complement(12328..13800)
                     /gene="purF"
                     /locus_tag="VNU01_04680"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWH31945.1"
                     /translation="MRQLPMHEPPRDHAPDPGKSGAREECGVIGVFARGEDVAKLCYY
                     GLYALQHRGQEAAGIAVSDGANILVTKEMGLVNQVFSEARLAGLDGHLGIGHVRYSTT
                     GASTWDGAQPTFKVTPTGHGIAVGHNGNLVNTADLARQLGPEADKCKTDSDILAALLA
                     RDADLSLEEAIVTTCNRLRGAYSLVIMDETTVYGARDPHGVRPLVIGRLPAGGYVIAS
                     ETAALDITGAHLVREVEPGELVAIDEKGLRSRRFAEATPAFCLFEYVYLARPDHRFPE
                     TSVYAARRRMGALLAEEAPVEADLVIPVPDSGTAAAAGYSEASGIPYAEGLVKNRYVG
                     RTFIQPSQSLRQLGIRLKLNPLRDILEGKRLVVVDDSIVRGNTSRQLVAMLRESGAAE
                     VHLRITAPPVRNPCFYGIDMATRAELVASGLTIEEIRDFVGSDSLAYLSLDGLVSASK
                     RPMNELCRACFDGEYPIPVPGEHAEAGKDVLGTLGAKAAR"
     gene            13938..14426
                     /locus_tag="VNU01_04685"
     CDS             13938..14426
                     /locus_tag="VNU01_04685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH31946.1"
                     /translation="MTVRERLVRWEDPAAAAEQGLALAGLDYLRGMIDGALPHPPICA
                     TLGFRVAEAEEGRCVIALEPGEHQYNPLGSVHGSVVVAALDSAAGNAVHSVLPAGVGY
                     TTVDLTTTFLRAVTAQTGTLYCEGTLIHAGRRIALSEAKLRDAEGTLYAHGTTTCMVL
                     GG"
     gene            complement(14434..15063)
                     /locus_tag="VNU01_04690"
     CDS             complement(14434..15063)
                     /locus_tag="VNU01_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31947.1"
                     /translation="MDSSSRWRAEPRTLLRLLAGLWLFGTGDALIVDAALGNAPWTVL
                     AQGVSLHTPLSIGVATQLIGVAVLLGWLPLRERPGLGTLANVVVIGLAIDAMRPLIPD
                     AGGLAARGAMLLAGTALVGVGSGFYLSAALGPGPRDGLMTGIHRRTGAPIALVRGAIE
                     LSALASGWLLGGTVGAGTVAFALLIGPAVQLGMRVAGARARTAEGAPRA"
     gene            15118..16785
                     /locus_tag="VNU01_04695"
     CDS             15118..16785
                     /locus_tag="VNU01_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWH31948.1"
                     /translation="MEALSQLADEFFELSLEAEPFAATVLGFHQFDTQVPDVTEAGQE
                     ALRARFAAVRDRAEALAGPGLSGEDEVTRAMLATMAGQTVDNIDARGVEFAVTPFWFG
                     AQAQVLTWLPKAEPADAEQAAAYVERCRRVPGFLDDSAERLRGGVSRGLVPVASGVAG
                     SLEQIADYLAMPLSEDLILGPLKDGPATDELREEAARVVADEVRPAMARFAEVLRTEI
                     APHARSDEQPGLVWLPDGQRRYEALMREHTSTEHTAKELHEIGLAQIARLDEEYRRLG
                     ERVLGTSDLQEIYRRLREDPELRYHEPADVVRDATQAIDRAREALPAWFGRLPEAPCE
                     VRPIPEMEAKGSTIAYYTPPPETGGPGVYWINTSTAASIFEAEAVAFHEALPGHHLQL
                     SIAQSLRGLPAFRRYSGVTAYVEGWGLYTERLADEMGLYSSDLMRIGMLSLDSLRAGR
                     LVVDTGLHAMGWSRERAIRFMVGNSPLDEQVVTSEVERYIAYPGQALAYMVGRLEILR
                     LREQAQGRLGDAFDIRAFHDVVLGSGAVPLTVLEDMVDRWAGQVAGS"
     gene            16844..17062
                     /locus_tag="VNU01_04700"
     CDS             16844..17062
                     /locus_tag="VNU01_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31949.1"
                     /translation="MTHHEQDEHRYERLDEEELRDSLAKAKAQDERLEREGTPEQVAE
                     HEERKRDLERKLEQVLAERRAGTAAEGR"
BASE COUNT         2422 a         6217 c         6239 g         2258 t
ORIGIN      
        1 gccttcccgg cggcctggac ttcgtgaact ggggcgggcg gtgacggcga cacttctctc
       61 cgacaccgaa ctggagcgcg accgccgcct ggtggaggcg catcaggcgg gcgacgtctc
      121 ggcgttcgga cagctgtacc ggctccacca cgagcgcttg ctgcgcttct gccagcgcaa
      181 gacgcgcgac ccccacctgg ccgaggagat cgcgcaggag gccttcctca aggcctaccg
      241 cgccctggac ggcctgaacg gcggcaagcg cttctatccc tggctgaccg tcatcgcctc
      301 ccggctggct atcgaccatc accgtcgcgc gggcagggtc cagccctcgg ccgcgatcga
      361 gctgggcgcg gtcgacgaca cccatgagcg catggaacgc caggtcgacc gggagcaggt
      421 cctccaggcg ctcgagcgcg tgcggctgcg ccaccgcgag gtcctgcggc tgcgcgacta
      481 cgaggacctg agctacgacg ccatcgcgca acggcttggc accagcccgg ccgtggtgca
      541 atcgctgttg caccgggcac gccaggccct ccggcgggag tatctggcgg tcaccgagca
      601 cgcgggcctc gtcccgctgc tgggtcccgc gtgcgccgcg ctccgccggc tgcgcgcgcg
      661 tgccgggcgg gcggcgtcct ggctgccgga cccctcggcc ttgggcgccc cgctggccgc
      721 ggccacactg gcgctgggcg gcgcgctggg cgcgtggacg gcacccgccc cgcagggcgc
      781 ggcctcctcg ggctcggccg ccgcggcggt cgagcccgcc accggccctg ctgcggcccg
      841 cctcgtggcg cagcccgcgc cggcggtcgg cgagccggcg ggcgtcccgc catcccggca
      901 gggacgaggc cctcgcgcgc cggccgaggt gtgggtgggg ccggccgggg ccgcagaggc
      961 gcgcgcgcac ggcgaggaca tgcccgcccg cgtggacacc ggccgggcga tcgtcgccgc
     1021 ggacccggaa accattctcg aggacacgct gcacgcggcc gccggcctgg tgaaaggtca
     1081 ctgacatgag cattcgccga gacctcgcgg tggcgctgct gctgctgctg ctcgcggcgc
     1141 tgggcgccgt gcccgcgcgg gccgcgctgg aggcacccgc cgcggcggcc gggcgctggg
     1201 gttgcgtcgc gctggacggg gtggccggat tctgcgtgag cagccccgtg gagtcctcag
     1261 ggctgtaggg ctcgtgacga gcggaacttt cttccaaaaa gaccgaaagg atccccccgc
     1321 tcgtccgttg ggactcgtgc gggggggccg cccagggtcc cgatggccga gccgaccggg
     1381 gggacgggtg cctgggcggc cttgcttcat ttcctcaggg gccaggacct gcgtccgagc
     1441 cgtccagccg cccgctccgg ccgcacaaca atcgctttac cggacacgtc cttgcgcgta
     1501 ctctaaaatc cggataccgg cgcgacaagg ggcccggggg ggttgatgag cgcgcttctg
     1561 gaggcgatcg aggacgaacg cgaccgcggc ctggtggagc gacaccaggc cggggacgac
     1621 gccgcgttct ccgagctgta cgccctgcac tacggccgcc tggtgcggtt ctgcaagcgc
     1681 ctggtgcgcg actcgcatgt cgcggaggaa ctggctcaag acgcgttcct gcgcgcgtac
     1741 acctcgatgg accgcctggc gggcgagcgg cgcttctacc cctggctcac cgtgatcgcg
     1801 cgccgtctgg tcatcgacca cgtccgccgt gacggacggg tgcagccgca cgccaacgtg
     1861 gagcccgtcc cagcgggcgc gaccgaggac gtggtcgtgc agcgcctcga cggcgaccac
     1921 ctgttgaccg ctttggaccg cgtccgcgac cgccatcgcc aggtcctgca cctgcgcgac
     1981 tgggaagggc tgagctacga ggccatagcc gatcggctcg gcctggcccc cacggccgtc
     2041 cctcccctgc tgcaccgggc ccgcctggcg ctgcggcgcg agtacatgct ggtcaccgag
     2101 ggccgcgtcg cggcgttcct gcccctgtcg gcggtcacgc tggccgtgcg ccggctgcgc
     2161 gaccgcgtcg ccctgtggac gagctggctg cccgacgccg gcgcgatctc cgctcccatg
     2221 gccggcgcgg tgctcgccgt gggcgcgctg ctcaccgtct cgccgggcgc cgcggacgcc
     2281 gcgggacacc gcgatgcggg caccgccgcc gcggtggagg acgggcacgc agccggctcg
     2341 ccggcgcagc tcggcgaggc gccgcgcgac acgacccacg gcctcggcgg tcggctggag
     2401 agggtgcctc ccccggcgcc tggcggcgag ccgcgcgagg tcctcgtgcc cgacgtcgcc
     2461 cacgtcaccc tggacccgcg accggacgcc gacggctcgc gcccccacga caaggagcag
     2521 ccgatctacc tcaatttcgg gcctgtcggc tatgccggtg acccggaacg gaccgcgggc
     2581 cacttcaagg gtgtcgcgga ggaccgcctg ccatgacaac agaacggggg ttacccatgc
     2641 gagcactgaa agccgccgta ctggggcttc tcgccgccac agtgctgttg gccggcctcg
     2701 ccggaccggc ggctgccgaa atcgactgcg tctggatcgc gggcaaccgc gtctgcgtgc
     2761 cgccggagta ggcgcgcggg cgatcacggc gcgcgcccac gggatccgtg aggcgcgcgc
     2821 ctctttcccc gtattccctg ccgcacgccg cgcacgtacc tttacggcgc gaagcatggg
     2881 gcgtaggctc gcatccggaa cacgatgtgg gaaggcacgg gggaaatggc cgcggtgctc
     2941 aacggctccg acctggagct ggaccgggac ctggtggaac ggcaccaggc cggcgacgac
     3001 gcggccttta cccagctcta cgccctgcac tacggccgcc tcgtccgctt ctgcctgcgg
     3061 ctgacgcgcg accaccacac cgccgaggag atcgcgcagg aggccttcgc ccgggccttc
     3121 gccacactgg accgcttcgg cggcgaacgg cgtttctatc cctggctgac cgtcatcgcg
     3181 cgccgcctgg tgatcgacca tgcccgccgc aacgagcgtg tggagaccag ggcgcacgtc
     3241 gacgccggct tcgccagccc cgccgaggac gtggtggtgc agcgcctgga cggccgtcac
     3301 ctcaccttcg cgatggaccg cctcaccgtg cgccatcagg acgtcatcag gctgcgcgac
     3361 gccgagggcc tgagctacga ggcgatcgcc gagcgcctgg gcgtccccgt gacgaccatc
     3421 ccgccgctgc tgcaccgcgc gcgcgccacg ctgcgccgcg agtacgtgcg cgcggaccgt
     3481 ggccgcgccg cctcctggct gcacctgtca ccggtgatcg ccgcggcccg gcggctccgc
     3541 gaccgactgg ccgtctgggg gtcgcacctg ccggacgcca gcatgttcgc ggcacccatg
     3601 gccggcgccg tcctgggcgc gaccgcgctc ctggcacccc aggtcatgat gggcgagcag
     3661 ggcgacgtcc ggttcggcgc gtccacgttc ggcctgcacg ttccggagcg cccgggggcg
     3721 ggccaggccg aggccgccgg ctcggccacg cagcccgcag agcatgccgg acaggaccac
     3781 ccggccggca acagcaaaga cttcgtcaac cccagctacg ccctgcggga cgcgggcgtc
     3841 cgcccggagg agacggtcct gcgctacgtc atggacagcg atcccgctac gtctgatgag
     3901 cgacaggacg aagtaagacg tatgccgatc tactacgaag aggaatacac cgggatttac
     3961 ctaggagtca atcccgaggc ggccgtacat tgtgtgcagt cgtcgtggag ttgcacggag
     4021 acgacgaggg aacgcgcggg gtccgcgctg gggcactagc cgaagccccc ccgggccctg
     4081 ctgggaccgg ggggatggac atgtccaaga agctgatcgt cgttgccgcg ctcgcactcg
     4141 cgcttttgca ggccacgcca gcgcaagcga gcgcctgctg catcatctgc gtcggggagc
     4201 ctccgaacgc ggtctgcatc ctgccggggt gcgacccgag ctgcacacca ctggtcgcgt
     4261 tagcccggga ccaggattgg tgacatctga gggtatggct gtacccgagg acctgctgga
     4321 catctacttc aaggagctcg gcggcgtcga gctcctcacg gccgcggacg agcaggagct
     4381 cgccaagcgc atggaggccg gccgcgccgc gcaggaacgc cttgcggcgg agcgcgccgg
     4441 caccgtcacg ctgtccgaac gcgagcgcgt cgagctgcgg acggccgccg acgcgggcgg
     4501 cgccgccttc gaccgcttcg tgtcggccaa cctgcggctg gtggtgagcg aggccgccaa
     4561 ggtcgcccgg cgcagcaagc tcgacctggg cgagctgatc caggagggca acctgggcct
     4621 gatcagggcg gtggagaagt tcgactggcg ccggggcctg aagttctcca cctacgcgac
     4681 ctggtggatc cgccaggcgc tccagcgcgg catggccggc aaggagcgca ccatccggat
     4741 ccccatgggc gtgcacgcca acctcatgaa ggtgcgcgcc gcggaggcgc ggctgtccaa
     4801 cgagctgaac cgcccgccgt gcctggacga gctgtcggac gcgaccaacc tcaccgagga
     4861 ccaggtgcgc gcggcgctgg aggcggactt cgccgtggcc tccctggaca agccggtcag
     4921 cgacgaccac gacgcggcgg acctcggggc gctggtggcg cgcgcgtccg acgcgcccgc
     4981 cgacgaggtc atcgaccgca tgttcgtgga gggcgtgttc gacgtggcgg cgcgccagct
     5041 tccgcagcgc tcctggtacg tgctgcagcg ccgctacggc ctggacggct tcgagccggc
     5101 caccctgcag gaggtcggcc gcgacatcgg cgtgtcccgc gagacggtgc gcaagatcga
     5161 gcagcaggcg ctggcgcacc tgcgcgacgc cgtgcaggtc gccgcgtgac ctgacgcccg
     5221 tgccgggccg ctagcctggg gccatggccc gcaagaagaa ggaacccggc aagcccggca
     5281 cgatctccca gctccgcatg gcgtggacgc agtcgcgcgc cctcgactcg cgcctggcgc
     5341 cctacacggc cgcggccgcg ctgctcggct tcgcggtgcc gttcgtcatc ctgctgctgc
     5401 tcggccggcc cgtcctcggc gcggtcgccg gcgtgctcgt ggcggcgctg gccgccctgt
     5461 cgcttttcgg ccgccgcgcc tccgcggtcc agctcacggc catcgagggg cagcccggcg
     5521 cggccgccgc ggtcgtgcag tcgctgcgcg gcatctggcg gctcacgccc gccgtcgccg
     5581 tcacccgcag ccaggacctg gtgcaccgcg tcgtcggccg accgggcatc gtgctcgtcg
     5641 gcgagggcgc gcccgctcgc gtggcgcagc tcttggcaca ggagaagcgg cgcgtggccc
     5701 gcgtggccaa ggacgtgccg gtgcacgagg tcagcgtggg caccggcaag ggccaggtgt
     5761 cgctcggcga cctgcgcatg cacatgatgc ggctgccgcg cgccgtgaag ccgcgtcaga
     5821 tcggcgagct ggagcgcaag ctgtcggcgc tgggcgagca gaacatcccg atccccaagg
     5881 gccccatgcc gacgcggcct ccgaagcgga tgcgctaggc cgccgaggcg ctcccgtcgc
     5941 cgcccgcgag cggcttgagc aggtccagca gttcccccac ggtgtgctgg cgttgcagca
     6001 cgtggcgcag cggacgctcg ggatggatcg cgtagacgtt gcgccggccg acgcgccgct
     6061 tgtcgatgta gccggcgtcg accagctccg acaggatgcg ctgcgccgcg cgctcggtga
     6121 tgccgacctg gtcggcgagg tcgcggaccc ggatctcacg gtccgccgcc acacacagca
     6181 ggaccagtgc gtggttcgtc agaaacgtcc agcgcgcgtc ggccgcaggc atccgcccct
     6241 ccacctcgac aagtcccgac ccatgattcc cgaattcacg ctgctgaacc atgccaccgc
     6301 cttccgccgc gtcaggacga ggacgggatg atggagatgg aaccgtcctt ctccagcacg
     6361 gcgtacttga tctgctcgag ccgctccagg ccctgcgacg agcgcgcctt ctccagcagg
     6421 tccgactcgc tgacgcgcgc cttgtccatt cggtccttga tcggctcgcc gtgctccagc
     6481 agcaccaccg gcagcccctc gacgtagcgg ctgacggcgg gcacacgctg ctggaacagc
     6541 gacatcccga tgtccatgag catcagggtg acgatcacga tgaacgcgtt cgtcatggag
     6601 aaatcctgcc ccagcagccc ctgctgcgtc gcctcgccga tgatgagcag cagcacgaag
     6661 tcgaacgtcg tggcctgcga cagcgtgcgg cgaccgctca cgcggaacac caccagcagg
     6721 aacaggtaca gggcggcggc gcggatgacg gcgtccatgg cggtctccta gggccagatg
     6781 aactgggaga agcggacggt cgacgtgccg acccgcacct cgccgcgcag cggacccatg
     6841 ccgtcggcct caaggtcgaa ctccaggcgc gcgcgagcgc cggcttgggg gaccgcgaag
     6901 acgaagcgca gcaggtcgcc gttggcctgg acctgctcgg gtgacggcgt gacgccccgc
     6961 agccgcatgt cctggaagta gcgcgccggc acgtcgacgg tgacctggcc gtcacggacg
     7021 tgggaggcgt ccacgcggat cgtcaggccg gcctcccgcc cacggcgcgc ccagcgcagg
     7081 tagtccaccg tcgccgactc gtcggcggac gccgcctcgg accagctcag cggaccgggg
     7141 cccagcaggc cgacgacggc cgccagcacc aggaggctca tgagcgtcca gccgacccgc
     7201 tgggccgccc attcccgcca ctggaagccc gcgtcctggt tgatctgcag gtccatgtcg
     7261 ccctccgtcc gcgcacgcgc cgcgcgaccg gagtgctttc ccgacctctg cttgacgaaa
     7321 cgggttacag gaacgggccg ttcggcaggt acgggtgcat ggcgccgagg cggccgcgtg
     7381 tgaacaccgc cgtgccgggg cgcaccagca gcccggcgcg gaagaacacg tcgaaggccc
     7441 agccctgctc cccggtcacg aagtaggtcg ccacgtcgcc gtccgattcg gccagtgccg
     7501 tccacagcag gcgctgcgcg gtcgccggtt ggtcggccgc cagcagcgcc gggctgccct
     7561 tgaacgtcat cacgaagccg cgatcccccg caccgtcgct cacccagaag ccgccgtcgg
     7621 gcccttcggc cagaaatgcg aggtccgggc cgtgcgacgc gccgcgcatg cgccggccga
     7681 ggtccgcgca cagctcgtag tcctcggggc gccccgggcg cacgcccgcg accgccggga
     7741 tcgcggcgcg gcgcacggcg cccttggcca gcgccgcggg cagaaggcgg aagccggcgg
     7801 cccagtagcg ccgccaggcg aaggggtcca gcgaggcggc gatgatgccg tccggccggt
     7861 ccccggcgta cgccagcgcg tgccgcatca gctcgcgccc gaccccctgg ccctgcgcct
     7921 cgccccgcac catcaacagc gacaggatcc acagatcctc gcgcagcagc gcgatcgccg
     7981 caccgatcag gcgcccgtcg cgctcggccg cccagcagcc gccggcgtcg tgcgcgacgc
     8041 tgtgggcgat gcggcgccgg tgcgccgcga gcatgcgctc gtcggcctgt ggcgggtcgt
     8101 cgtgggcgcg ttccgcggcg gcggcgaagg cgtcggtcgt cacgtcatag acgtcgtcgg
     8161 cctcgtcggc gcgcagcggc ctcacgtgga aggacgccgt cacgcggcgc cctcctgccc
     8221 ggccgtcggc tccagcggga cgggggcgtc ccacacctcg tcgtggaaca gcgccggtgc
     8281 gtcgcggccc tccaccgtca ccacgacctg gcgggcgtcc gggaagtagt gcgcgaccat
     8341 cgcaaggaat tgggccttgg ccgcctggcc gccggcgctg ccgagctggt tcgtcgccga
     8401 gatgcccgcc ccgaagtcca gcaccgccgt ggggccgccg tcacggtctt ccacgccggc
     8461 cgacagcagc cgcaactgca ccccctccag cgggtgcagc aggtcgacgg ggccctcgcc
     8521 ctccagcgcc gcgaacgccg cgcgggcaag ttcctcccgc gtggggtctg gcgacaggcc
     8581 ccacgccttg cggtggcgcg gctccatgtc ggccaccgtc ggcccgtcca gcgcctcggg
     8641 ggcgaaccag ccggccagcc acaccgtgtc gacgtccgcc tcgacctgcg cctgggccat
     8701 ctcgacgtcg tcgtaccagg tggtgtcctc gggggccgcc ggcaactcgc cggggactgc
     8761 cgcctgggtc tggccctgct gcccgtcggc ggggctcggt ggcgtgccgc ccgcgcacgc
     8821 gaccatgctg agcgtggcga gcagcaccaa ggctgcgggg atcgagcggc gcatccatgc
     8881 tcctgtcggt gtggcctccg gcgcttcgga cgcacgggac agcggacagg ttgccatgga
     8941 ccaccgcgcg cacgacaaca agcccgcggt ctcacgggct cgccgcagtg cgccgaatgg
     9001 ccggcgccgc gaggaactga gtggcgtccc gccggagcgg cccgcgctcc tctcccccac
     9061 cggagaggat cgtggacgcc cggcgccact gcctcaggcc gccgaggcgg ccaagggcac
     9121 tagatcagct gctggccctc gagcagggca cgaacctccg acgcgcggta ccggcggtga
     9181 ccgccgagcg tgcgaatgga ggacagcttg ccggacttcg cccagcgggt cactgtcttg
     9241 gggtcgacgc ggaacagctt ggccacctcg gaaggtgtca gcagctcttc gtcgacactg
     9301 gtcgtcgctt tcacgttggc ccacctccac ctgtccgccc gaccgcgccg gaggcggtca
     9361 atccaagccg gacaagccca accgggacaa agcgtaaggc ccaagcgggg gagaaggaaa
     9421 taccccattc gcaggaaatt tgtgacgtac gtccggaccc gttgccccgc gcggccccac
     9481 gcgggaggat cacaggcacc catcccaccg ttgcgaccag gagatccggc acatggactc
     9541 tgtcgttttc gaccacttcg acggccacga gcaggtggtc ttcgcggccg atcccgcgag
     9601 cggcctgcgc tgcatcgtgg ccattcattc caccgcactg ggacccggcc tgggcggcac
     9661 ccgcttcaag gcctacccga cggaggaggc cgcgctggtg gatgtgctgc gcctgtcgcg
     9721 agccatgacg tacaagaacg cctgcgccgg gctcgaccac ggcggcggca aggcggtgat
     9781 catcggcgac ccggcggtgc tgcgatccga ggcgctgctg cgcgcgtacg gccgtgtcat
     9841 cgcgtcgctg ggcggccgct acatcaccgc gtgcgacatc ggcaccaagc ccgcggacat
     9901 gtcggtgatc cgccacgaga cgcggtgggt gaccggcatg gaccctgcgg acggcggctc
     9961 gggggattcc ggcgtgctga ccgcctgggg tgtctacgcc ggcatcaagg cgtgcctgca
    10021 gcacgtgtcc ggcgaccgct cactggcggg gcgccatgtc gcggtgcagg gggtcggcaa
    10081 ggtgggccgg cggctggcgg agcacctggc cgcggatggc gcgcgcctca ccctggccga
    10141 tgtggacgag ccggcggtgc gcgacctcgc ggcgcggctg aacgcggaag tcgtgcccgt
    10201 cgaggcggtc catcgcgtgg actgcgacgt gttcagcccc aacgccctgg gcgcggtgct
    10261 gaacgacgac accatcccgg agctgtcctg ccgcgtggtg gcgggcggcg cgaacaacca
    10321 gctggccgag gagcggcacg ccaagctcct gcacgagcgc ggcgtcctgt acgcgccgga
    10381 cttcgtgatc aacgccggcg gggtgatcca gatcagcgac gagctgcacc cggccggcca
    10441 ctcacccgag cgcgccaaga cgaagaccga gacgatcgcc gaccgcctgc tggagatctt
    10501 cgcccacgcc gaggccgagg gcatcgacac cgagcaggcc gccaacgccg tcgccgagaa
    10561 gcgcatcaac gccgtcggca gattgcgcag cttctggctc tcggagtagc gacgggtccg
    10621 gctcggcatc cctcggagcc tctccaggga acccgcctcg gcgctgcgcg cctcggcacc
    10681 ctgagtgtcg gctcctcggg cgccgctccg gaccccgcgt tgctactccg cgtgctggtg
    10741 cgagccttct tcagcgtgct gcgactcgtg ctcggcgatc tggcggcgca cgtcgtccat
    10801 gtccagcgcc ttggcctgct ggatgaggtc ctccagggct tcctccggga ggctgccggg
    10861 ctgggagtac agcagcacct ggtcgcggaa cagcatcagg gtcgggatcg acatgatctg
    10921 gaaggcggcg gccagctcct gctcggcctc cgtgtccacc ttgccgaaca cggcgtcggg
    10981 gtggcgctcc gacacgcgct cgaacacggg ggcgaagctc cggcacggcc cgcaccactc
    11041 cgcccagaag tccacgagca ccagcccctc cgcgtccagc acggtctgct ggaacgtgtc
    11101 agcggtcagc tcaacggtgg ccatgcctac tcctcgcgtc tacggctaca tctgtttgct
    11161 ccccggccaa cgccccgggc attcaccctg ttccatagcg atgccagcgg tcgcggccgc
    11221 gcccgaggag ccgacactca gggtgccgag gcgcgcagcg ccgaggcggg ttccctggag
    11281 aggctccgag ggatgcggac gtgaccgcag gaccctatac ggacaggcga acgcccgggg
    11341 tgtcggtcac cgcaccacac acccatgcgt cgacgccgcg ctgcgcgagc aggtccaccg
    11401 cggcgcggcc gtcgggcacg acggccagca tgccggcgcc catgttccag acgcgccagc
    11461 actcgtcctc cgccacgcgg ccccactcct gcagcttcct gaagaccgct gggatctcga
    11521 acgtggacgt gtccacggtg gcgccgagac cctccgggag gacgcgcggg aggttgcccg
    11581 ggatgccgcc gccggtgacg tggcacagcg cgtgcacgtt gccggacgcg gccagcgcca
    11641 ggcagtcctt ggtgtagatg cgcgtggggc gcagcagcgc ctcgcccagc ggcatgccca
    11701 ggccgtgctc gccgtgcaag tccttgccgt cgacgatcct gcggatgagc gagtagccgt
    11761 tgctgtgcgg gccgctggag gccatggcca cgatctggtc gcccgcctgc acccgctccg
    11821 ggccgagcat gcggtcgcgt tcgacgacgc cgacgccgaa gccggcgagg tcgtactggc
    11881 cggccggcat gaccccgggg tgctcggcgg tctcgccgcc caccagcgcg cagcccgcct
    11941 cgacgcagcc ctgcgcgatg ccggcgacga tctccgccag gtgcgccggg tcgagcttgc
    12001 cgacggccac gtagtcgagg aagaacagcg gctcggcgcc ggtgaccacg atgtcgtcga
    12061 cgaccatggc caccaggtcg acgccgacgg tgtcgtggcg gccgagctgg cgggccagct
    12121 cgatcttggt gccgaccccg tccgtgccgc tgaccagcac cgggtgcttc cagcggccga
    12181 ggtccagttc gaacaggccg ccgaaccccc cgatgccgcc cagcacgccg gcgcggtgcg
    12241 tgcgcgcgac gtgctccttc agcagctcga cggacgcgtc ggcggcctcc agcgacacgc
    12301 ccgcgtcgga gtaggtgagc ccgtcgctca ccgggcggcc ttggcgccga gcgtgccgag
    12361 gacgtccttg cccgcctcgg cgtgctcgcc gggcacgggg atggggtact cgccgtcgaa
    12421 acaggcgcgg cacagctcgt tcatcggccg cttgctggca ctgaccagcc cgtccagcga
    12481 caggtaggcc agcgagtcgg agccgacgaa gtcgcggatc tcctcgatcg tcaggcccga
    12541 ggcgacgagt tcggcgcgcg tggccatgtc gatgccgtag aagcaggggt tgcgcacggg
    12601 cggcgcggtg atgcgcaggt gcacctcggc ggcgccggac tcgcgcagca tggccaccag
    12661 ctggcgggag gtgttgccgc ggacgatgga gtcgtccacc accaccaggc gcttgccctc
    12721 aaggatgtcg cgcaaggggt tgagcttgag ccggatgccg agctggcgca gcgactggga
    12781 cggctggatg aacgtgcgcc cgacgtagcg gttcttgacc aggccctcgg cgtaggggat
    12841 gccgctggcc tcgctgtagc cggcggcggc cgcggtgccg ctgtccggga cggggatcac
    12901 gaggtccgcc tcgaccggcg cctcctcggc caggagcgcg cccatgcggc gccgcgcggc
    12961 gtacacggac gtctccggga agcggtggtc ggggcgcgcc aggtagacgt actcgaacag
    13021 gcagaacgcc ggggtcgcct cggcgaagcg gcggctgcgc aggcccttct cgtcgatcgc
    13081 gacgagctct cccggctcca cctcgcgcac caggtgcgcg cccgtgatgt cgagggcggc
    13141 ggtctcgctg gcgatgacgt agccgccggc ggggagccgg ccgatgacca gggggcgaac
    13201 cccgtggggg tcgcgggcgc cgtagacggt ggtctcgtcc atgatcacca gcgagtacgc
    13261 gccgcgcagg cggttgcacg tggtgacgat ggcctcttcc agggacaggt cggcgtcgcg
    13321 ggcgagcagc gccgccagga tgtcgctgtc ggtcttgcac ttgtccgcct cggggcccag
    13381 ctgacgggcc aggtcggcgg tgttgaccag gttgccgttg tgcccgacgg cgatgccgtg
    13441 gccggtcggg gtcaccttga aggtgggctg ggcgccgtcc caggtggacg cgccggtggt
    13501 ggagtagcgc acgtggccga tgcccaggtg cccgtccagg ccggccaggc gcgcctcgct
    13561 gaagacctgg ttcaccaggc ccatctcctt ggtgaccagg atgttggcgc cgtcgctgac
    13621 cgcgatgccc gccgcctcct ggccgcggtg ctgcagcgcg tacaggccgt agtagcacag
    13681 cttggcgacg tcctcaccgc gggcgaacac gccgatgacg ccgcactcct cgcgggctcc
    13741 gctcttgccg gggtccggcg cgtggtcacg gggcggttcg tgcatcggga gctggcgcat
    13801 gagaacgggc accccagagc gagaggcggc atggggaagg accaggcgag tgtatccggg
    13861 aggggcgccg ccgaccgacc cccacgccgc gtcgcggatt gcgatgatgc gcaggacttc
    13921 ggcgacggga gagcgggatg acggtgcggg aacggctggt ccgctgggag gacccggcgg
    13981 ctgcggcgga gcaagggctc gccctggcgg ggttggacta cctgcgcggc atgatcgacg
    14041 gtgcgctgcc gcatccgccg atctgcgcga cgctcggctt ccgcgtcgcc gaagcggagg
    14101 agggccgctg cgtgatcgcg ctggagccag gcgagcatca gtacaacccg ctcggcagcg
    14161 tgcacggcag cgtggtggtg gccgccctgg actcagcggc cggcaacgcg gtgcactccg
    14221 tgctccccgc aggggtcggc tacaccaccg tggacctgac caccacgttc ctcagagccg
    14281 tcacggcgca gaccggcacc ctgtactgcg agggcaccct catccacgcc ggccgcagga
    14341 tcgccctctc cgaggccaaa ctgcgcgacg cggaaggcac gctctacgcg cacggcacca
    14401 cgacctgcat ggtcctcggc ggctgaaccg cggtcaggcg cgcggcgcgc cctcggccgt
    14461 gcgcgcacga gcgcctgcca cacgcatgcc caactgcacc gcggggccga tcaggagggc
    14521 gaacgccacc gtcccggcgc cgacggtgcc gccgagcagc cacccgctgg ccagggcgct
    14581 caactcgatg gcgccgcgca ccagggcgat cggcgcaccg gtgcgccggt ggattcccgt
    14641 catcagcccg tcgcgcggtc cgggtcccag ggccgcgctc aggtagaagc cgctgcccac
    14701 gccgaccagc gccgtgccgg ccagcagcat cgcgccgcgc gcggcgagcc cgccggcgtc
    14761 cgggatcagc gggcgcatgg cgtcgatcgc caggccgatc accacgacgt tggcgagcgt
    14821 ccccagcccg gggcgttcgc gcagcggcag ccaaccgagc agcaccgcca cgccgatcag
    14881 ctgcgtggcc acgccgatgg acagcggcgt gtgcagcgag acgccctgcg ccagcacggt
    14941 ccacggcgcg ttgccgagcg ccgcgtcgac gatgagcgcg tcgccggtgc cgaacagcca
    15001 caggccggcg agcaggcgca ggagcgtgcg gggttcggcg cgccagcgtg aggacgaatc
    15061 catcccgcga gcgtacggtg tggccttccc cacgccgggc gagaagtgga gcggaaagtg
    15121 gaagcattgt cacagctggc cgacgagttc ttcgaattga gcctggaggc ggagccgttc
    15181 gccgccacgg tgctgggctt ccatcagttc gacacgcagg ttcccgacgt cacggaagcc
    15241 ggccaggaag cgctgcgggc gcgcttcgcg gccgtccgcg accgcgccga ggccctggcc
    15301 gggccggggc tgtccggcga ggacgaggtc acgcgggcca tgctcgccac gatggcgggc
    15361 cagacggtcg acaacatcga tgcgcgcggt gtggagttcg ccgtcacgcc gttctggttc
    15421 ggcgcgcagg cgcaggtgct gacgtggctg ccgaaggcgg agccggcgga cgccgagcag
    15481 gccgcggcct acgtcgaacg ctgccgcagg gtgcccgggt tcctggacga cagcgcggag
    15541 cggctccggg ggggcgtgtc ccgcgggctg gtgccggtgg ccagcggcgt ggcgggctcg
    15601 ctggagcaga tcgccgacta cctggccatg cccctgagcg aggacctgat cctggggccg
    15661 ctcaaggatg ggccggccac cgacgagctg cgcgaggagg ccgcccgcgt ggtcgccgac
    15721 gaggtgcgtc cagccatggc gcgcttcgcc gaggtgctgc gcacggaaat cgccccgcac
    15781 gcgcgctccg acgagcagcc gggcctcgtg tggctgccgg acggccagcg ccgctacgag
    15841 gcgctcatgc gcgagcacac cagcaccgag cacaccgcca aggaactgca cgagatcggc
    15901 ctggcgcaga tcgcgcggct ggacgaggag taccggcgcc tgggcgagcg cgtgctgggg
    15961 acctccgacc tgcaggagat ctaccggcgg ctgcgcgagg acccggaact gcgctaccac
    16021 gagcccgccg acgtggtgcg cgacgcgacg caggcgatcg accgcgcgcg cgaggcgctg
    16081 cccgcgtggt tcggcaggct cccggaggcg ccctgcgagg tccggccgat cccggagatg
    16141 gaagccaagg gcagcacgat cgcctactac acgccgccgc cggagacggg cgggccaggc
    16201 gtgtactgga tcaacacctc gaccgccgcg tcgatcttcg aggcggaggc ggtcgccttc
    16261 cacgaagcgc tgcccgggca tcacctgcag ctgtcgatcg cccagtcgct gcgcggcctg
    16321 ccggcgttcc ggcgctactc cggagtgacc gcctacgtcg agggctgggg cctgtacacc
    16381 gagcggcttg ccgacgagat ggggctgtac tccagcgacc tcatgcgcat cggcatgctg
    16441 tcgctggact cgctgcgggc cgggcgcctc gtggtggaca cgggactgca cgcgatgggc
    16501 tggagccgcg agcgcgcgat ccgcttcatg gtgggcaaca gcccgctgga cgagcaggtg
    16561 gtcaccagcg aggtggagcg ctacatcgcc taccccggcc aggcgctggc ctacatggtg
    16621 gggcgcctgg agatcctgcg cctgcgcgag caggcgcagg gccggctggg cgacgcgttc
    16681 gacatccgcg ccttccacga cgtggtgctc gggtccggcg cggtgccgct cacggtgctg
    16741 gaggacatgg tggaccggtg ggccggacag gtggcgggca gttaggcgcg cgtggggcca
    16801 gggtaggcag tcatcccgtc ccgaacgccc acaggagcgc gagatgaccc accacgagca
    16861 ggacgagcac cgctacgagc ggctggacga ggaggagctg cgcgactcgc tggccaaggc
    16921 gaaggcgcag gacgagcgtc tggagcgcga gggcacgccc gagcaggtcg ccgagcacga
    16981 ggagcgcaag cgcgacctcg agcgcaagct cgagcaggtg ctcgccgagc gccgggccgg
    17041 cacggccgcg gagggccgct gatgcccggt ctgcccgtga agcccaggaa gctggtggcc
    17101 ggggcgctcg gcctgaccgc gaagggactg gagatc
//
LOCUS       DATMKC010000157         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_57970, whole genome shotgun sequence.
ACCESSION   DATMKC010000157 DATMKC010000000
VERSION     DATMKC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57970"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..655
                     /gene="bioD"
                     /locus_tag="VNU01_04705"
     CDS             32..655
                     /gene="bioD"
                     /locus_tag="VNU01_04705"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWH31950.1"
                     /translation="MRGFLVTGTDTGVGKTLVSALICRARPGATYVKPAQTGLAQDSA
                     DAEIVAKLSGAATRQGPGLPEPLAPAVAAEREGRAISRAELLAPFSGLDDVVGEGAGG
                     LLVELGTDGTTLADLALDLDLPLVVVARPSLGTLNHTRLTCEAAWARGIRLAGIVVNG
                     YPQDAGVAEETNLVQLGRMAPVLDVVPWLPPDSLLPSLGAWASAERP"
     gene            690..1733
                     /gene="bioB"
                     /locus_tag="VNU01_04710"
     CDS             690..1733
                     /gene="bioB"
                     /locus_tag="VNU01_04710"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWH31951.1"
                     /translation="MNPHQLLDEVEAALLDRRELITEAQAYALADLADLVDGKEDPST
                     TQWDDPLPRMLDLAHRVRLAWCGEEVSLESILSTKTGGCPEDCAFCSQSSTFNTLVKP
                     QPYLTREQVVSAAKDAEASGATEFCLVLAIKGPDERAMAHLLEATEAIQSETRLEVAL
                     SAGLLTREQAQALAEAGVVRYNHNLEAAESFFPQICTTHSWKERWQTCQYVRETGMEV
                     CSGGILGMGESARQRAELALALRELEPTEVPVNFLNPRPGTPLEDQTILDPRMALHGV
                     ALFRLIIPFAMLRYAGGREVVLGDLQAAGILGGANGMIVGNYLTTLGRTVSEDIKMLD
                     ELQVPVKALTGLL"
     gene            1733..1936
                     /locus_tag="VNU01_04715"
     CDS             1733..1936
                     /locus_tag="VNU01_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31952.1"
                     /translation="MDTVAGAFCGACGGAAGAADHAGCAQRLRYDPPRFCAACGFRLD
                     VQVYPAGVVSSCRRCERPARKAR"
     gene            2059..2370
                     /locus_tag="VNU01_04720"
     CDS             2059..2370
                     /locus_tag="VNU01_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31953.1"
                     /translation="MALVLGVLGAVLAAPAAAAPQPVDRVERNGVMYTVYEERKPGDP
                     NTYLYYVEDGALPGTSGGLRFSARQNGMESAAFVAATLLFAVSLLAVYRRGTMQGLLH
                     G"
     gene            complement(2434..>3310)
                     /locus_tag="VNU01_04725"
     CDS             complement(2434..>3310)
                     /locus_tag="VNU01_04725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH31954.1"
                     /translation="RSWVWERGGEVVAHWAAVSVPLVLDGRPAVGVKTADAATHPDHR
                     RQGLFATLALRYMQDLRERGIPAVLTHPNPDSAAGVEASGPVLVERLPAYVLAVDDAW
                     LARRFHVPASVSRLVRRAAFPAPGAGGQEVAAPPEGLDALWSGLGERWGVRRDAAWWR
                     WRYQAHPAARYRFAEVRRGGALRAAAAVRVQEAFGGRFAYVMEFLAADQEAAGALTGT
                     LADLAREQGAAGLVVVTTSWSRTAALVRGAGFRRLPRRLEPNPLRFLVAEPGGDSAGL
                     ARQRWSAAWGDLDHL"
BASE COUNT          462 a         1263 c         1193 g          392 t
ORIGIN      
        1 ccgacgtgct gtcgggccgg ggagtgcgcg cgtgagaggc ttcctggtca ccgggaccga
       61 cacgggcgtg ggcaagaccc tcgtcagcgc gctcatctgc cgcgcccgcc cgggcgccac
      121 ctacgtcaag ccggcgcaga ccggcctggc gcaggacagc gccgacgcgg agatcgtcgc
      181 caagctgagc ggcgcggcca cgcgccaggg gcccggcctg ccggagccgc tggcgccggc
      241 ggtcgccgcc gagcgcgagg ggcgcgccat ctcccgcgcc gagctgctcg ccccgttctc
      301 cggcctggac gacgtggtcg gcgaaggcgc cggcggcctg ctggtggaac tcggcaccga
      361 cggcaccacg ctggccgacc tggccctgga cctcgacctg ccgctggtcg tggtggcgcg
      421 ccccagcctg ggcaccctca accacacgcg gctgacctgc gaggcggcgt gggcgcgcgg
      481 catccgcctg gcgggcatcg tcgtgaacgg ctacccgcag gacgccggcg tggccgagga
      541 gaccaacctc gtgcagctcg gccgcatggc gccggtgctg gacgtggtgc cctggctgcc
      601 gcccgactcg ctgctgccct cgctcggcgc atgggcatcc gccgagcgcc cttagaatcc
      661 ggcgaccgcc cagtgacagg agaacgcccg tgaatccgca ccagctgctc gacgaggtcg
      721 aggccgcgct gctggaccgc cgcgagctga tcaccgaggc gcaggcctac gccctcgccg
      781 acctggccga cctcgtcgac ggcaaggaag accccagcac gacccaatgg gacgacccct
      841 tgccccggat gctggacctg gcccaccgcg tgcggctggc ctggtgcggc gaggaggtca
      901 gcctggagtc gatcctgtcg accaagacgg gcggctgccc ggaggactgc gccttctgct
      961 cccagagctc caccttcaac acgctggtca agccgcagcc ctacctgacg cgcgagcagg
     1021 tcgtgagcgc ggccaaggac gccgaggcca gcggcgccac cgagttctgc ctggtgctgg
     1081 ccatcaaggg acccgacgag cgcgccatgg cgcacctgct ggaggccacg gaggcgatcc
     1141 agagcgagac ccggctggaa gtggcgctgt ccgcgggcct gctgacacgc gagcaggcgc
     1201 aggcgctcgc cgaagcgggt gtcgtccgct acaaccacaa cctcgaggcc gccgagagct
     1261 tcttcccgca gatctgcacc acgcacagct ggaaggagcg ctggcagacc tgccagtacg
     1321 tgcgcgagac cggcatggag gtgtgctccg gcggcatcct cggcatgggc gagagcgccc
     1381 ggcagcgcgc cgagctggcg ctggcgctgc gcgagctgga gcccaccgag gtccccgtca
     1441 acttcctcaa cccccggccg ggcacgccgc tggaggacca gaccatcctg gacccgcgca
     1501 tggccctgca cggcgtggcg ctgttccgcc tgatcatccc gttcgcgatg ctgcgctacg
     1561 ccggcggccg cgaggtcgtc ctgggcgacc tgcaggcggc cggcatcctg ggcggggcca
     1621 acggcatgat cgtgggcaac tacctcacca cgctgggacg caccgtctcc gaggacatca
     1681 agatgctcga cgagctgcag gtcccggtga aggcgctgac cgggctgctg tgatggacac
     1741 cgtcgcgggc gcgttctgcg gcgcgtgcgg cggtgcggcc ggcgccgccg accacgccgg
     1801 ctgcgcgcag cggctgcgct acgacccgcc gcgcttctgc gccgcgtgcg gcttccgcct
     1861 ggacgtgcag gtctaccccg cgggggtggt gtcgtcctgc cggcgctgcg agcgtcccgc
     1921 ccgaaaagcc cgttaaaagc ggaaccgggc gccgcccggg cgcgttatcc tccagacctg
     1981 gctccgggcg tgctcacgcc cggcgggaag ccgacagcga cacacaagag gcggacgatg
     2041 ctgcgcagga ctctcggatt ggctctggtt ctcggcgtgc tgggggccgt gctcgccgcg
     2101 cccgcggcgg cggccccgca gcccgtggac cgcgtcgagc gcaacggcgt gatgtacacg
     2161 gtgtacgagg agcgcaagcc cggcgacccg aacacctatc tgtactacgt cgaggacggc
     2221 gcgctgccgg gcaccagcgg cgggctgcgc ttctccgccc gccagaacgg catggagagc
     2281 gcggccttcg tggcggcgac gctgctgttc gccgtgtcgc tgctcgcggt gtaccgccgc
     2341 ggcacgatgc aaggcttgct ccacggctga ccgcccgcat ctgcgggacc acttgtggcc
     2401 gctatcggac acaagtggtc ccggaagcgg caattacagg tggtcgaggt cgccccacgc
     2461 cgctgaccag cgctgccggg ccagccccgc ggagtcgccg cccggctcgg ccaccaggaa
     2521 gcgcagcggg ttcggctcca gccgcctcgg cagccgccgg aagcccgcgc cgcgcaccag
     2581 cgccgcggtc cgcgaccacg atgtcgtgac caccaccagg ccggcggcgc cctgctcgcg
     2641 cgcgaggtcg gcgagcgtcc cggtgagcgc gccggcggcc tcctggtcgg ccgccaggaa
     2701 ctccatcacg tacgcgaagc ggcccccgaa ggcctcctgg acgcgcaccg ccgccgccgc
     2761 acgcagcgcg ccgcctcgcc gcacctccgc gaagcggtag cgggcggccg ggtgcgcctg
     2821 gtagcgccag cgccaccagg cggcgtcacg ccggacgccc cagcgctcgc cgagccccga
     2881 ccacagtgcg tccaggcctt cgggcggcgc ggcgacctcc tgcccgcccg cgccgggagc
     2941 cgggaaggcg gctcggcgga ccagcctgga cacggacgcc ggcacgtgga agcgccgggc
     3001 cagccaggcg tcgtcgactg ccagcacgta cgccgggagg cgctcgacca gcacggggcc
     3061 ggaggcctcc acacccgccg ccgaatcggg gttcgggtgc gtgagcaccg ccgggatccc
     3121 gcgctcgcgc aagtcctgca tgtagcgcag cgccagcgtg gcgaacagcc cctggcggcg
     3181 atgatcggga tgggtcgcgg cgtccgccgt cttcacgccc acggcggggc ggccgtccag
     3241 caccaacggc accgagaccg cggcccagtg cgccaccacc tcgccgccgc gctcccacac
     3301 ccaggagcgc
//
LOCUS       DATMKC010000158         5065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_129768, whole genome shotgun sequence.
ACCESSION   DATMKC010000158 DATMKC010000000
VERSION     DATMKC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129768"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..2050
                     /locus_tag="VNU01_04730"
     CDS             200..2050
                     /locus_tag="VNU01_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWH31955.1"
                     /translation="MAVTGGLRGLCATLLPPEHGGPDPSVLAGQVAAYLDAAPGRVRL
                     GMLAAAAALDGLALASRRRRLVDLAPGERVRVLESLASRSPEVEQALLGLKAAVLLVA
                     GTEQYRDELNREAGATDLANPEPELDVVPASDWPDGVTADVVVVGSGAGGAMAARTLA
                     RAGMRVLVLEEGRRWSVEEFRTGHPLERFAGLYRDAGTTTALGRPPVVLPIGRAVGGT
                     TVVNSGTCYRTPAAVQDAWRAKGVATEDFEARLDEAEATFKVAPAPLDVLGRNGHLAL
                     AGARALGWQAAPLRRNAPGCGGCCQCAIGCPRNAKYGVHLNALPQACEAGARIVSDAH
                     VTRIVVEGGRAAGVRARRPDGSEFTVRAPRVVMACGTTESPHLLWRSGLGSHPEVGRH
                     LAIHPAIGIAGRFAEPVVAWKGVLQSAGVEEFHHSDGILIEATSTPPGMGSVTLPGYG
                     VELLRELDQADHLATIGALVADDGNGRVVGRRGGRKLIRYDLGERDAGRLRKAVTVMG
                     ELLLAAGAEEILTGIPGVPAARTVEGLRDAVWDVPARRLHLAAFHPTGTLAMGADAQR
                     YPVDPGGRLRGVDGVWVADGSILPSSPTVNPQITIMALALGVAADVAATP"
     gene            complement(2162..3073)
                     /locus_tag="VNU01_04735"
     CDS             complement(2162..3073)
                     /locus_tag="VNU01_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWH31956.1"
                     /translation="MKRVAAVDVGTNSVRLLVAEPADGGIAHLERLMTVTRLGQGVDA
                     SGRLHPDALRRTLDCVAGYAARWAELGVTRVRIAATSAVRDAANRDAFFDGVRDIAGI
                     DAEVLSGAEEARTAFRGATASLDAPAPFLVLDIGGGSTEFILGTTRPEADTSRQLGCV
                     RLTERCLGGDPPAAAQLDEARAVVDAELDAVEALFEPRAARTLVGVAGTVTTIGALHL
                     ELPEYLPERIHGARVPVDGVRAIFERLAEQTSAQRARLGPMAKGREDVIVGGALILLR
                     VMERFGFDEVLVSEADILDGLAMSLLD"
     gene            complement(3070..3621)
                     /locus_tag="VNU01_04740"
     CDS             complement(3070..3621)
                     /locus_tag="VNU01_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HWH31957.1"
                     /translation="MLRPPQDRDPYAEVLAKPYADAGELALIEALLGRPSKARAAVAV
                     RCANGLPAVLRCDPYDLDGNPFPTMFWLCAPLAASRIGGLESTGVMSDLTDAVREDTE
                     FGRHNREATARFLALRDRLPGGPIPGNPTQGGMPDRVKCLHSLYAHYLATGDNPVGEW
                     AHQQLAPVPCHAECVRADQEPQA"
     gene            complement(3644..3880)
                     /locus_tag="VNU01_04745"
     CDS             complement(3644..3880)
                     /locus_tag="VNU01_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31958.1"
                     /translation="MPYFLMVYDRQAARILELREFAEAERDDAMRLRDAREETEMDNE
                     RIEVVLFGAESEDALRRTHGRYFGLSGTLAARKQ"
     gene            complement(3891..4592)
                     /locus_tag="VNU01_04750"
     CDS             complement(3891..4592)
                     /locus_tag="VNU01_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31959.1"
                     /translation="MSGRFSKAEIDRLGERIRASERLSVEDLRILQAWRAQHADALDE
                     VVRILVQDLGLHPTSRLKTQDTLIDKLHRETTRLSQVQDVAGCRVEVDGGLAEQDDVV
                     AGVIARFPEAKVFDRRERPSHGYRAVHIVVATAHGLVEIQVRTRLQNLWAQIIERFAE
                     IWGRQIQYGGEPDAPEPLRPYAHGLLDTLHGLSVSAAGLEVRGLADEGSIRALEAVLQ
                     RAEAFVSSRRPAAEP"
     gene            complement(4602..>5065)
                     /locus_tag="VNU01_04755"
     CDS             complement(4602..>5065)
                     /locus_tag="VNU01_04755"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWH31960.1"
                     /translation="LADPAAKRPAAKRGKTKRQPPRGVRKAVKKATRGDRPYLMGLVL
                     LAFVLMSMAWAPIKNLAAGSARIDQLTASRDALAHEVDKLEERKDELMDPEQLELIAR
                     EEYGLVKPGEIPYVVVTPEADPRLRPVDEAPEVAEVPWYRRVWSAIRRLWE"
BASE COUNT          673 a         1831 c         1879 g          682 t
ORIGIN      
        1 gccgtggccc gctccagccg ccgggcggtg tcctggtcgg acaccagctt ggagatcgcg
       61 gcgcccgacg ccaccagcat cggcgcgtcc atcagaaagg ccatgcgcgt ctcctcgtcc
      121 ctcgcggcag cccgtctgcc gtgacctgca gtcagtatgc tgccccgaac ggagcatttc
      181 gacggcgatc gaggcaacca tggcggtcac cggcgggctg cggggcctgt gcgccacgct
      241 gctgccgccc gagcacggcg ggccggaccc gtccgtgctg gccggccagg tcgccgccta
      301 cctggacgcc gcaccggggc gcgtgcggct cgggatgctg gccgcggccg ccgcgctgga
      361 cgggctggcc ctggcatcgc gacgccggcg cctggtggac ctggcgcccg gggagcgcgt
      421 gcgggtgctg gagtcactgg cctcccgctc ccccgaggtg gagcaggcgc tgctcggcct
      481 gaaggcggcg gtgctgctgg tggccggcac cgagcagtac cgcgacgagc tgaaccgcga
      541 ggccggcgcc acggacctcg ccaaccccga gcccgagctg gacgtcgtgc ccgcgagcga
      601 ctggccggac ggcgtcacgg ccgacgtcgt ggtggtgggc tcgggcgcgg gcggcgccat
      661 ggcggccagg acgctggccc gggccggcat gcgcgtcctg gtgctggagg agggccggcg
      721 ctggagcgtc gaggagttcc gcaccgggca cccgctggag cgcttcgcgg ggctgtaccg
      781 cgacgccggc accacgaccg cgctgggccg tccgcccgtg gtgctgccga tcggccgcgc
      841 cgtggggggc accacggtgg tcaacagcgg cacgtgctac cgcacgccgg cggccgtcca
      901 ggacgcctgg cgcgccaagg gcgtggcgac ggaggacttc gaggcgcgcc tggacgaggc
      961 ggaggcgacc ttcaaggtgg cacccgcgcc gctggacgtg ctggggcgca acgggcacct
     1021 ggcgctggcg ggcgcccgcg cgctgggctg gcaggccgcg ccgctgcggc gcaacgcgcc
     1081 gggctgcggc ggctgctgcc agtgcgccat cggctgcccg cgcaacgcca agtacggcgt
     1141 gcacctcaac gctctgccgc aggcgtgcga ggcgggcgcc cgcatcgtga gcgacgcgca
     1201 cgtcacgcgc atcgtggtgg agggcggccg cgcggccggg gtgcgcgccc ggcggcccga
     1261 cggctcggag ttcaccgtca gggcgccgcg cgtggtgatg gcctgcggca ccaccgagtc
     1321 gccgcacctg ctgtggcgca gcgggctggg cagccacccg gaggtgggcc gccacctggc
     1381 catccacccg gccatcggca tcgccgggcg cttcgcggag ccggtcgtgg cgtggaaggg
     1441 cgtcctgcag agcgcgggcg tggaggagtt ccaccactcc gacgggatcc tcatcgaggc
     1501 gacgtcgacg ccgcccggga tgggctcggt cacgctgccc ggctacggcg tggagctgct
     1561 gcgggaactg gatcaggccg accacctcgc gaccatcggg gcgctggtcg ccgacgacgg
     1621 caacggccgc gtggtcggcc gccgcggcgg gcgcaagctc atccgctacg acctcggcga
     1681 gcgcgacgcg ggccggctgc gcaaggcggt gaccgtcatg ggcgagttgc tgctggccgc
     1741 cggcgcggag gagatcctca ccggcattcc cggcgtgccg gcggctcgga ccgtggaggg
     1801 actgcgcgac gcggtctggg acgtgcccgc gcggcggctg cacctggcgg cgttccaccc
     1861 gaccggcacg ctggcgatgg gcgccgacgc gcagcgctac ccggtggacc ccggcgggcg
     1921 gctgcgcggc gtggacggtg tgtgggtcgc cgacgggtcg atcctgccga gctcgcccac
     1981 ggtcaacccc cagatcacga tcatggcgct ggccctgggt gtggccgccg acgtggccgc
     2041 caccccgtag cgcgaccccg tagcgccgcg ccaaccggcc ctcaccgcgc cgccgccttc
     2101 cgcccacagt gggcgcaaac cccgagatac gggggtaacg gttcacaacc cggtggcgag
     2161 gttagtccag gagggacatc gcgaggccgt cgaggatgtc ggcctcgctc accagcacct
     2221 cgtcgaagcc gaagcgctcc atcactcgca gcaggatcaa ggcgccgccc acgatgacgt
     2281 cctcgcgccc cttcgccatc ggtccgagcc gagcccgctg ggcggaggtc tgctcggcca
     2341 ggcgctcgaa gatggcgcgc acgccgtcca cggggacgcg ggcgccgtgg atgcgctccg
     2401 gcaggtactc cgggagctcc aggtgcaggg ccccgatggt cgtcaccgtg ccggccacgc
     2461 cgaccagcgt gcgggcggca cgcggctcga acagcgcctc cacggcgtcc agctccgcgt
     2521 ccacgacggc gcgcgcctcg tcgagctggg cggcggccgg cgggtcgccg ccaaggcagc
     2581 gctcggtcag gcgcacgcag ccgagctggc ggctggtgtc ggcctcgggg cgcgtggtgc
     2641 ccaggatgaa ctcggtggag ccgccgccga tgtccaggac caggaacggc gcgggcgcgt
     2701 ccagggaggc ggtggcgccg cggaaggccg tgcgtgcctc ctcggcgccg ctgagcacct
     2761 ccgcgtcgat gccggcgatg tcgcgcacgc cgtcgaagaa ggcgtcgcgg ttggcggcgt
     2821 cgcgcacggc gctggtcgcg gcgatgcgca cgcgcgtcac gcccagctcg gcccagcgcg
     2881 cggcgtagcc ggcgacgcag tccagcgtgc ggcgcagcgc gtccgggtgc agccggccgc
     2941 tggcatccac gccctggccc aggcgcgtga ccgtcatgag gcgctcgagg tgggcgatgc
     3001 cgccgtcggc aggctcggcc acgagcaggc ggacggagtt cgtgccgacg tccacggccg
     3061 cgacgcgctt catgcctgcg gctcctggtc agcgcgcacg cactcggcgt ggcacggcac
     3121 gggcgcgagc tgctggtgcg cccactcccc cacggggttg tcgccggtgg cgaggtagtg
     3181 cgcgtacagc gagtgcaggc acttcacgcg gtcgggcatc ccgccctgcg tggggttgcc
     3241 cgggatgggg ccgccgggca ggcggtcgcg cagggccagg aagcgcgccg tggcctcgcg
     3301 gttgtggcgg ccgaactcgg tgtcctcccg cacggcgtcg gtgaggtcgg acatgacgcc
     3361 ggtcgactcc aggccgccga tgcggctggc cgccaggggg gcacacagcc agaacatcgt
     3421 ggggaacggg ttgccgtcga ggtcgtaggg gtcgcagcgc agcaccgccg gcaggccgtt
     3481 ggcgcagcgg acggcgacgg cggcccgcgc cttcgagggg cgccccaaca gcgcctcgat
     3541 cagcgcgagc tcgcccgcgt ccgcgtacgg cttggccagc acctcggcgt agggatctcg
     3601 gtcctgtggc ggccgcaaca tgtcctgcat agggtggggc gacctactgc ttacgcgccg
     3661 cgagggtgcc gctcagtccg aagtaccgcc cgtgcgtccg ccgaagcgcg tcctcagact
     3721 cggcaccgaa aagcacgact tcgatgcgct cgttgtccat ctcggtctcc tcgcgagcat
     3781 ctcgaagtct catcgcgtca tcgcgctcag cctccgcgaa ttcccgaagt tcgaggatgc
     3841 gtgcggcctg gcggtcatag accatgagga agtagggcat gcggaccatc ctagggctcc
     3901 gcggccggtc tgcggcttga tacgaacgcc tctgcgcgtt gcagaaccgc ttcgagagca
     3961 cggatagacc cttcatccgc aagaccacgc acctccagcc cagcggcaga gaccgacaag
     4021 ccgtgcagag tgtccagcag gccatgggcg tacggtctta agggctctgg ggcatccggc
     4081 tcgccgccat actggatttg ccgaccccaa atctctgcga agcgctcgat gatctgcgcc
     4141 cagaggttct gcaggcgagt gcgcacctgg atctccacca ggccatgcgc ggtcgcaacg
     4201 actatgtgaa ccgcacggta accgtgactc ggacgttcgc ggcggtcgaa caccttcgcc
     4261 tccgggaacc gagcaatgac gcccgccacg acgtcgtcct gctccgccaa ccctccgtcc
     4321 acttcaacgc ggcagccggc gacatcctgt acctgactga gccgcgtcgt ttcgcggtga
     4381 agcttgtcaa taagggtgtc ctgcgtcttc agccgcgatg tcggatgcaa cccaaggtcc
     4441 tggacaagta tccgcaccac ctcgtccaaa gcgtccgcgt gctgggcgcg ccaggcctga
     4501 aggatgcgca gatcttcgac cgacagccgc tcggaggcgc ggatgcgctc gcccaggcgg
     4561 tcgatctctg ccttcgagaa ccgaccgctc accgcgcccc cttactccca gagtctcctg
     4621 atcgccgacc agacgcgacg gtaccagggg acctcggcca cctcgggggc ttcgtccaca
     4681 ggccggagcc tggggtcggc ctcgggggtg accaccacgt aggggatctc gcccggcttg
     4741 accaggccgt actcctcgcg cgcgatgagc tcaagctgct cggggtccat gagctcgtcc
     4801 ttgcgctcct caagcttgtc cacctcgtgc gcgagcgcgt cgcggctggc ggtgagctgg
     4861 tcgatgcgcg ccgagcccgc ggccaggttc ttgatcggcg cccacgccat ggacatcagc
     4921 acgaacgcca gcagcaccag gcccatgagg tagggccggt cgccccgggt cgccttcttg
     4981 accgccttgc ggaccccgcg gggcggctgg cgcttggtct tgccgcgctt cgcggccggc
     5041 cgcttcgccg cggggtccgc aaggc
//
LOCUS       DATMKC010000159         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_310531, whole genome shotgun sequence.
ACCESSION   DATMKC010000159 DATMKC010000000
VERSION     DATMKC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310531"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..254)
                     /locus_tag="VNU01_04760"
     CDS             complement(6..254)
                     /locus_tag="VNU01_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31961.1"
                     /translation="MRKNLLLAAMIAALAFAGVACSDDDTAVEDEGTGTEVEDVTEDE
                     GAADESMDEGAADETTDETTDEGATDDAAVEGEAEVTE"
     gene            complement(449..1543)
                     /locus_tag="VNU01_04765"
     CDS             complement(449..1543)
                     /locus_tag="VNU01_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWH31962.1"
                     /translation="MRIAYSDHLVALLCQGEAAAARLAGADPDRRAATVAAARRESAL
                     LSARLDASPLEPSTAEAVDARDAAGLPPLEDLPPPPPQERARAGSWARALKLEDLPTQ
                     DVAALEYANLLTTFDAEPGIAGWFFERPLDALAELHRLLTHGLVAPEDAGRPRRTEQA
                     VHDGAQGKVIYNTPAPEAVPGLLEELAGWLTGAGGRLGSAALPGVLVAGVVHERLLEW
                     QPFEAANGRLARCAARVVLRARGVDPHGCAVVERELARDVAAYHREVAATMRRRDLGP
                     WLERWAEAALVALEDAADAVDPRPRPEPPARARELLDELRPDEPLTIADYARRAGVTF
                     ASARADLHALVAAGGLRRDLGSRGLRFRRI"
     gene            complement(1547..>2216)
                     /locus_tag="VNU01_04770"
     CDS             complement(1547..>2216)
                     /locus_tag="VNU01_04770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWH31963.1"
                     /translation="LPRMLKDAGSSIVFRLLGASDEKSEALRDRILASVAGTPQEELL
                     RFMPAVVEELIEKVRPEAQALVDMHMEAGRDVWIISASPVEVVAELARALGMTGGLGT
                     ESEVVDGVYTGRLAAPFCYGEGKAERIHKLAAERGYDLSKCYSYSDSASDLPMMQVVG
                     HPVAVNPDRALTAVAHRRGWPVVEFARQAKRIARLSGYTLGTAGLAGGTYLLGRRHGA
                     RRAG"
BASE COUNT          267 a          849 c          823 g          277 t
ORIGIN      
        1 agtgattact cggtgacctc agcctcgccc tcgacggcgg cgtcgtcggt cgcgccctcg
       61 tcggtggtct cgtccgtggt ctcgtcggca gcgccctcgt ccatcgactc gtcggcggcg
      121 ccctcgtcct cggtcacgtc ctcgacctcg gtgccggtgc cctcgtcctc gaccgcggtg
      181 tcgtcgtcgg agcaggcgac gccggcgaac gccagggcgg cgatcatggc ggcaagcagc
      241 aggttcttgc gcatgtggag tcctccaaga gatctcgtgt tcgggcgtgg gcgacggccg
      301 ccccgctcgg ctccgggctg gcggccttga cggcaactac agcggtagga gctggtgcta
      361 tccaagcgca cgcgaggtcg tggacggtgc aaccgaccct gtccggcagg acaggagggg
      421 acaacccccc aagcgcgccg aatcccggtc agatccggcg gaaacgcagg ccgcgcgaac
      481 cgaggtcgcg ccgcagcccg cccgcggcga ccagggcgtg caggtcggcg cgcgcgctgg
      541 cgaacgtgac gccggcccgg cgggcgtagt cggcgatggt gagcggctcg tccggacgca
      601 gctcgtcgag caactccctg gcgcgcgccg gcggttccgg ccgggggcgc gggtccacgg
      661 cgtccgccgc gtcctccagc gcgaccagcg ccgcctccgc ccagcgctcc agccacggcc
      721 ccaggtcgcg gcggcgcatg gtcgccgcga cctcacggtg atacgccgcg acgtcacgag
      781 ccagctcgcg ctccacgacc gcgcagccgt gcgggtccac cccgcgggcg cgcagcacca
      841 cgcgggcggc gcagcgcgcc agccgcccgt tggcggcctc gaacggctgc cactccagca
      901 gccgctcgtg caccacgccg gcgaccagca cgcccggcag cgccgccgag cccaggcgcc
      961 cgccggcccc ggtcagccac ccggccagct cctccagcag gccgggcacg gcctcgggcg
     1021 cgggggtgtt gtagatgacc ttgccctgcg cgccgtcgtg gacggcctgc tcggtccggc
     1081 gcggccgccc ggcgtcctct ggggcgacca gcccgtgggt gagcagccgg tgcagttcgg
     1141 ccagcgcgtc cagcggccgc tcgaagaacc agccggcgat gccgggctcc gcgtcgaacg
     1201 tcgtgagcag gttggcgtac tccagcgccg cgacgtcctg cgtcggcagg tcctccagct
     1261 tcagggcgcg cgcccagctc cccgcgcgtg cgcgctcctg cggaggcggc ggcggcaggt
     1321 cctccagcgg cggcaggccg gccgcgtcgc gggcgtccac ggcctcggcg gtggacggct
     1381 ccagggggct cgcgtccagg cgggcggaca gcagggcgga ctcgcgccgg gcggccgcga
     1441 ccgtggcggc gcgccggtcc gggtcggcgc cggccaggcg cgcggcggcg gcctcaccct
     1501 ggcacagcag cgcgaccagg tgatcgctgt aggcgatgcg catgcctcag ccggcgcggc
     1561 gcgcgccgtg gcggcggccc agcaggtacg tgcccccggc gagcccggcg gtgcccagcg
     1621 tgtagccgga cagccgcgcg atccgcttgg cctgccgcgc gaactcgacc accggccagc
     1681 cgcggcggtg cgccacggcg gtgagcgcgc ggtcggggtt caccgccacg ggatggccga
     1741 ccacctgcat catgggcagg tcgctggcgc tgtcggagta ggagtagcac ttggacaggt
     1801 cgtagccgcg ctcggccgcc agcttgtgga tgcgctccgc cttgccctcg ccgtagcaga
     1861 acggcgcggc cagccggccg gtgtagacac cgtcgacgac ctcgctctcc gtgcccaggc
     1921 cgccggtcat gcccagggcg cgcgcgagct cggcgacgac ctccacgggg ctggccgaga
     1981 tgatccagac gtcccggccc gcctccatgt gcatgtcgac cagcgcctgc gcctccgggc
     2041 gcaccttctc gatcagttcc tcgacgacgg cgggcatgaa gcgcagcaat tcctcctgcg
     2101 gggtgccagc cacgctggcc aggatgcggt cgcgcagcgc ctcgctcttc tcgtcggagg
     2161 cgccgagcag gcggaacacg atcgagctgc cggcgtcctt cagcatgcgc ggcagc
//
LOCUS       DATMKC010000160         5285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_160298, whole genome shotgun sequence.
ACCESSION   DATMKC010000160 DATMKC010000000
VERSION     DATMKC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160298"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1246
                     /locus_tag="VNU01_04775"
     CDS             146..1246
                     /locus_tag="VNU01_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH31964.1"
                     /translation="MAAGEALRAEGLAVGYRARRHERLVLDGLDVALRPGRLTCLLGP
                     NGSGKSTLLRTLAGMQAPLAGRALLGGQDVHRLAAMERARRLAVVLTDQVDAGTLRVE
                     DLVALGRHPHTGWSGRLTARDADAVSAAITAVDAGHLRGRMVSELSDGERQRVLIARA
                     LAQQPRVMALDEPLAFVDVPRRVELTQLLRRLAREADLAILLTTHDLDLALRFADDLW
                     LLDGARLLRGGPEDLVLSGAVARAFAAQDVSFDLERGAFLPVAAPRAVARVHGSGAGA
                     VWLARALERAGLRVASPDDPRPAAFEAVELFQGGFGLEVAAGPDDGGSWSEHPTIESL
                     LETVEAALRRAGPAPGSGGCRPRAGDPPARSA"
     gene            complement(1170..2321)
                     /locus_tag="VNU01_04780"
     CDS             complement(1170..2321)
                     /locus_tag="VNU01_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31965.1"
                     /translation="MTTGPLEHPLQWVSLALGAVLVVSTGMSLLGTLVLPRAQRDDVA
                     RNVERVVRAALSLLARLRRGYEGKDAVLALHGPLALAGMLVTWLCLLLVGWTMVLWAV
                     TEGSIGDAAREAGSSMFTLGFASRNTWAATIADFLASASGLLVVALLIAYLPVLYAAF
                     TRREALVTMLQSRAGVPAWGPEILARHQSVGLLRTLGAFYVDWERWIADVAESHTTYP
                     ILVSFRSPHALRSWVTSLLAVLDAAALQHALQGEQAPSQARLCLRMGFTALRDIADVL
                     GIPYDPDPSPDDGITLSYEAFCKGVDRVEGTGFPMMRTREEAWPHFQGWRVNYESIAY
                     AIADMVTAPPAPWSGPRHGLRGATITPIVPVDRRPGGDTRRTPAPDPHA"
     gene            complement(2318..3217)
                     /locus_tag="VNU01_04785"
     CDS             complement(2318..3217)
                     /locus_tag="VNU01_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH31966.1"
                     /translation="MSYQSIFRPGLFDGQVALVTGGGTGIGRCIAHELAALGATVVLA
                     ARREEQLEATAAEIAESGGRADWHPVNIRDEASVDALVAEVMARHGRVDLLVNNAGGQ
                     FPSPAEGISPNGWRTVIDLNLTGTFLVTRAVFNASMAERGGAVVTITADVRNGFPGMS
                     HTGAARAGVENLTKTLAVEWASRHVRVNAVAPGTIYSSGMDTYAEQFQRIAAQGARRI
                     PAGRVGTESEVSAAVCYLLSPAAGFVTGVTLRVDGGAPLQKIPMIDVGAVRSTEPYDG
                     FHLAREVPAYWSAPEPGAEGGPE"
     gene            complement(3214..3606)
                     /locus_tag="VNU01_04790"
     CDS             complement(3214..3606)
                     /locus_tag="VNU01_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWH31967.1"
                     /translation="MSADPAQRRRISSGSPYEPVIGFSRAVVAGDRVIVSGTAPVWPD
                     GSCDPDPAAQARRCLEIILAALAEAGAGPEHVVRTRMFLTDAADADAVGAAHGEVFGD
                     VRPAATMVVVKALLDERWKVEIEAEAIR"
     gene            3721..>5285
                     /locus_tag="VNU01_04795"
     CDS             3721..>5285
                     /locus_tag="VNU01_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31968.1"
                     /translation="MSHYKSNLRDIEFNLFEVFKVQEHLGTGAFGQIDADTARNILSE
                     IERLALNEYSDSFVPADRTPLKLDPETGDVTIPAEVSKALDAFHAGQWNLLDVPEHLG
                     GYNAPPSVRWASLELLLGANPVPVMYTAGPVFAAIMDPLVTDEQRERWVRPMVERNWG
                     ATMVLTEPDAGSDVGAGRTKAVQAEDGTWHITGVKRFITNGDFDWPENIVHLVLARPE
                     GAGPGTKGLSLFIVPKFWVNEDGSLGERNGAYCTNIEDKMGIKASATCEMTFGDRHPA
                     RGVLVGEVHDGIAQMFKVIEYARMMVGTKAIATLSTGYLNALEYAKTRVQGADLKQMT
                     DKTAPRVEIIRHPDVRRSLMIQKAHAEGMRALVMYAASIQDQVKLAEAAGDHEAAAEW
                     EKLNDLLLPMVKGYGSEKSYELLAQSLQTFGGSGFTRDYPIEQYIRDAKIDTLYEGTT
                     AIQGMDLFFRKVARDQGATLMRLAGQIAEFAKGDAGNGTLAAERALLGKALEEVQGMV
                     GALVGFFAKDLYL"
BASE COUNT          738 a         1938 c         1949 g          660 t
ORIGIN      
        1 ccatcgccct ggcggccggg ctcatcgccc tgctgcccgg cagcgacgcc agcctgccgc
       61 tgaacgccgt gacgtcgctg ttcggcgcgc cggtcgtggt ggggatcctg ttgcggctgc
      121 ggcgcagcgg caaggcggtg agctgatggc ggccggcgag gcgctgcgcg ccgagggcct
      181 ggcggtcggc taccgcgccc gccgccacga gcggctggtg ctcgacggcc tcgacgtggc
      241 gctgcggccg ggccggctca cctgcctgct ggggcccaac ggcagcggca agtcgacgct
      301 gctgcgcacg ctggcgggca tgcaggcgcc gctggcgggc cgggcgctgc tgggcggcca
      361 ggacgtgcac cgcctcgccg cgatggagcg ggcacggcgc ctggccgtgg tgctcacgga
      421 ccaggtcgac gccggcacgc tgcgggtgga ggacctggtg gcgctgggac gccaccccca
      481 caccggctgg tcgggccggc tcaccgcgcg cgacgcggac gcggtgagcg cggcgatcac
      541 cgccgtggac gccgggcacc tgcgcggccg catggtgtcg gagctgtccg acggcgagcg
      601 ccagcgcgtg ctgatcgccc gcgcgctggc gcagcagccg cgggtcatgg cgctggacga
      661 gccgctggcg ttcgtggacg tgccgcgccg cgtggagctg acgcagctgc tgcgccggct
      721 ggcgcgcgag gccgacctgg cgatcctgct caccacgcac gatttggacc tggcgctgcg
      781 cttcgccgac gacctgtggc tgctggacgg cgcccgcctg ctgcgcggcg gtcccgagga
      841 cctggtgctg tcgggcgcgg tcgcgcgcgc cttcgccgcg caggacgtca gcttcgatct
      901 ggagcgcggc gcgttcctgc ccgtggcggc gccgcgcgcg gtggccaggg tgcacggctc
      961 cggcgccggc gcggtgtggc tggcgcgcgc gctggagcgc gcgggcctgc gcgtggcctc
     1021 ccccgacgac ccccgcccgg cggcgttcga ggcggtggag ctgttccagg gcggcttcgg
     1081 cctggaggtg gccgccgggc cggacgacgg cggctcgtgg agcgagcacc ccaccatcga
     1141 gtcgctgctg gagaccgtcg aggccgcgct caggcgtgcg ggtccggcgc cggggtccgg
     1201 cgggtgtcgc ccccgggccg gcgatccacc ggcacgatcg gcgtgatcgt cgcgccgcgc
     1261 aggccgtggc gcggcccgct ccacggcgcc ggcggcgcgg tcaccatgtc ggcgatcgcg
     1321 taggcgatcg actcgtagtt gacgcgccac ccctggaagt gcggccaggc ctcctcgcgc
     1381 gtgcgcatca tcgggaagcc ggtcccctcc acgcggtcca cgcccttgca gaacgcctcg
     1441 tagctcagcg tgatgccgtc gtcgggcgag gggtccgggt cgtaggggat gcccagcacg
     1501 tcggcgatgt cgcgcagcgc cgtgaacccc atgcgcaggc acagccgcgc ctggctgggc
     1561 gcctgctccc cctgcagcgc gtgctgcagc gccgcggcgt ccagcaccgc cagcagcgac
     1621 gtgacccacg agcgcagcgc gtgcggcgag cggaagctca ccaggatcgg gtaggtcgtg
     1681 tggctctcgg ccacgtcggc gatccagcgc tcccagtcga cgtagaacgc gcccagcgtg
     1741 cgcagcagcc ccacggactg gtgccgcgcc aggatctccg gcccccaggc cggcacgccc
     1801 gcgcggctct gcagcatcgt gaccagcgcc tcgcgccgcg tgaacgccgc gtacagcacc
     1861 ggcaggtagg cgatcagcag ggcgaccacc agcaggccgc tcgcgctggc caggaagtcg
     1921 gcgatcgtgg cggcccaggt gttccgcgac gcgaacccca gggtgaacat ggacgagccg
     1981 gcctcgcgcg ccgcgtcgcc gatggagccc tcggtcaccg cccacagcac catcgtccag
     2041 cccaccagca gcaggcacag ccaggtcacc agcatccccg ccagcgccag cggcccgtgc
     2101 agcgcgagca cggcgtcctt gccctcgtag ccgcggcgca ggcgcgccag cagcgacagc
     2161 gccgcccgca ccacgcgctc gacgttgcgc gccacgtcgt cgcgctgcgc gcgcggcagc
     2221 accagggtgc cgagcaggct catgccggtg gagaccacca gcaccgcgcc cagcgccagg
     2281 ctgacccact gcagcgggtg ctccagcggc ccggtggtca ctcgggcccg ccttcggcgc
     2341 ccggctcggg cgccgaccag tacgccggca cctcgcgcgc caggtggaag ccgtcgtagg
     2401 gctcggtgga gcgcaccgcg ccgacgtcga tcatcgggat cttctgcagc ggcgcgccgc
     2461 cgtccacgcg cagggtcacg ccggtcacga agccggcggc gggcgacagc aggtagcaca
     2521 ccgcggcgga gacctcgctc tccgtgccga cgcggccggc cgggatgcgc cgggcgccct
     2581 gcgccgcgat gcgctggaac tgttcggcgt aggtgtccat gccgctggag tagatggtcc
     2641 cgggcgcgac ggcgttgacg cgcacgtggc ggctcgccca ctccaccgcc agcgtcttgg
     2701 tgaggttctc cacgccggcg cgcgcggcgc cggtgtgcga catgccgggg aagccgttgc
     2761 gcacgtcggc ggtgatggtg accacggcgc cgccgcgctc ggccatggag gcgttgaaca
     2821 cggcgcgcgt caccaggaac gtgccggtga ggttcaggtc gatcaccgtg cgccagccgt
     2881 tgggcgagat gccctcggcg ggcgagggga actgcccgcc ggcgttgttg accagcaggt
     2941 cgacgcggcc gtggcgcgcc atcacctcgg cgaccagcgc gtcgacggac gcctcgtcgc
     3001 ggatattcac gggatgccag tccgcgcggc cgcccgactc ggcgatctcg gccgccgtcg
     3061 cctcgagctg ctcctcgcgg cgcgcggcca gcaccacggt ggcgcccagc gcggcgagct
     3121 cgtgggcgat gcagcggccg atgccggtgc cgccgccggt gaccagcgcc acctggccgt
     3181 cgaacagccc cggccggaag atcgactggt agctcaccgg atcgcctcgg cctcgatctc
     3241 gaccttccag cgctcgtcca gcagcgcctt gaccaccacc atcgtcgcgg cggggcggac
     3301 gtcgccgaac acctcgccgt gcgccgcgcc gaccgcgtcg gcgtcggcgg cgtcggtcag
     3361 gaacatgcgc gtgcgcacga cgtgctcggg ccccgcgccc gcctcggcca gggccgccag
     3421 gatgatctcg aggcagcgcc gcgcctgcgc cgcggggtcc gggtcgcagg agccgtccgg
     3481 ccacaccggc gccgtgccgg acacgatcac ccggtccccg gcgacgacgg cccgcgagaa
     3541 gccgatcacc ggctcgtagg gggacccgga ggagatgcgg cgccgctggg cgggatctgc
     3601 gctcatggcc gcgcagacta ttgcgtggaa aagctaccgg cgggtaacct actggctggt
     3661 aaccggtccg gggcccgggc cgggcaggaa gtcgccggcg tccatcgagg ggaaaccgca
     3721 atgtcccact acaaaagcaa cctgcgcgac atcgagttca acctgttcga ggtgttcaag
     3781 gtgcaggagc acctgggcac gggagcgttc ggccagatcg acgccgacac cgcgcgcaac
     3841 atcctcagcg agatcgagcg cctcgccctc aacgagtact ccgacagctt cgtgccggcg
     3901 gaccgcacgc cgctgaagct ggacccggag accggcgacg tgaccatccc cgcggaggtc
     3961 tccaaggccc ttgacgcctt ccacgccggc cagtggaacc tgctcgacgt cccggagcac
     4021 ctgggcggct acaacgcccc gccgtcggtg cgctgggcgt cgctggaact gctgctgggc
     4081 gccaaccccg tgccggtcat gtacaccgcc ggcccggtgt tcgccgcgat catggacccg
     4141 ctggtcaccg acgagcagcg cgagcgctgg gtccgcccga tggtcgagcg caactggggc
     4201 gcgaccatgg tgctcaccga gcccgacgca ggctcggacg tgggcgccgg gcgcaccaag
     4261 gccgtgcagg ccgaggacgg cacctggcac atcaccggcg tgaagcgctt catcaccaac
     4321 ggcgacttcg actggccgga gaacatcgtg cacctggtgc tggcccgccc cgagggcgcc
     4381 ggccccggca ccaagggcct gagcctgttc atcgtgccga agttctgggt gaacgaggac
     4441 ggctcgctgg gcgagcgcaa cggcgcctac tgcaccaaca tcgaagacaa gatgggcatc
     4501 aaggcctcgg ccacgtgcga gatgaccttc ggcgaccgcc accccgcgcg cggcgtgctg
     4561 gtcggcgagg tgcacgacgg catcgcgcag atgttcaagg tcatcgagta cgcgcgcatg
     4621 atggtcggca ccaaggccat cgccacgctg tccaccggct acctgaacgc gctggagtac
     4681 gccaagacgc gcgtgcaggg cgccgacctc aagcagatga cggacaagac ggcgccgcgc
     4741 gtggagatca tccgccaccc cgacgtccgg cgcagcctca tgatccagaa ggcgcacgcg
     4801 gagggcatgc gcgcgctggt gatgtacgcc gcgtccatcc aggaccaggt caagctggcc
     4861 gaggccgccg gcgaccacga ggccgcggcc gagtgggaga agctcaacga cctgctgctg
     4921 cccatggtga agggctacgg ctcggagaag agctacgagc tgctggcgca gtccctgcag
     4981 accttcggcg gctccggctt cacgcgcgac tacccgatcg agcagtacat cagggacgcc
     5041 aagatcgaca ccctctacga gggcaccacg gcgatccagg gcatggacct gttcttccgc
     5101 aaggtcgccc gcgaccaggg cgcgacgctg atgcgcctgg ccggccagat cgccgagttc
     5161 gccaagggcg acgccggcaa cggcacgctg gcggccgagc gcgcgctgct gggcaaggcg
     5221 ctggaggagg tgcagggcat ggtgggcgcg ctggtcgggt tcttcgctaa agacctgtac
     5281 ctgat
//
LOCUS       DATMKC010000161         7204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_295801, whole genome shotgun sequence.
ACCESSION   DATMKC010000161 DATMKC010000000
VERSION     DATMKC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295801"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1248
                     /locus_tag="VNU01_04800"
     CDS             58..1248
                     /locus_tag="VNU01_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWH31969.1"
                     /translation="MRELTDPDWVELVGGVPRWLPDGRLLHTVDEEDTRRLAADGVAL
                     TPPGLQVRRVAGVQDGHVVFTASREPTATELYRVALAGGDPEPLTAPGGMADGAVGGP
                     SLVTVRRSLERSGPEVVVRRGGVARAVAGAALPHGLELHVRLLELGPRALRAALVLPA
                     GHGPGDGPLPVLLDPYGGPHAQRVVQAADAYLTSQWFADQGFAVLVADGRGTPARGPA
                     WEKAVAGDLASAPLEDQVDALHAAAEAVPGALDLSRVAIRGWSFGGFLAALAVLRRPD
                     VFHAAIAGAPVTDWSLYDTHYTERYLGTPQDDAARYRRSSLLDDAAGLARPLLLIHGL
                     ADDNVVAAHTLRLSGALLAAGRPHEVLPLSGVTHMTPQEVVAENLLRLQLEFLRRHLE
                     RPRT"
     gene            1304..2170
                     /locus_tag="VNU01_04805"
     CDS             1304..2170
                     /locus_tag="VNU01_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31970.1"
                     /translation="MTTSHARADDGGDVTRLARLRGTYTPGTVLIGPSGIGKSVLVNR
                     FAARSAAEGDVVLDAVRVARRSDPVAQLAGVVRAAAGQIASGRRHGTLGELMDRVQLI
                     AIKGVQFAVREGVGNPHLSVRDALVHLGEVLVRENVTRRKPRALLVRLDELQNADDTQ
                     RSALLTAIGDLLEHTVELDLPGGRLPMHLPVLVYVTGLPDLLNRATNVDTFRRRFDTR
                     KLTLFVDQEVTSSLLGAQLPDRVRFTDEAAARLAELVSGDPYLCQYVGQAAWNGRRRR
                     SRPPPDGSNSAR"
     gene            2383..4197
                     /locus_tag="VNU01_04810"
     CDS             2383..4197
                     /locus_tag="VNU01_04810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWH31971.1"
                     /translation="MRALEAGEHELAFWQRHVRIGVSLSQLTLGVCLVYTLRYADPSR
                     RGVLLALIGIAFASTLGVNLLPLRRILSGRAAVPFFAAWSALLTLLIVLGSLGDGGGD
                     TPLALLYFMPVVYGAIAYPPGGVIALGIAVVGAAVVTRIVGGGSPGGTLMLGGTLALV
                     TTMCAVSARNHWRTTRSQTALAERLAHLADHDGLTGCLNHRAFHERLRQEAARAVRYE
                     RALGLLLVDLDGFKAVNDGHGHPCGDAVLAAVGDVLRATTRDTDIVGRVGGDEFAVLL
                     PETGCDEAHLLAERIRAAVGALREPVPVTASIGVAALPPAHDAATLLAAADVAVYQAK
                     RSGRDYVVVHGDQPAARQQGGSHALLRERVHEVVDGGRLTALFQPIVCLADGSVLGYE
                     ALTRVDESSLGPAQWLDLAERAGVRDSLEAAMWRTALAAGPPPDAAVLFLNAGPEALL
                     SGAMAEFRGRLGPHAAIEVSEHDAIADYGLLSGALAPWIGDGARIAVDDMGAGHANLA
                     HVLNLGPHYLKLDRSLVEGLHEHPSRRALLESLITFAARIGSELVAEGVELEQEAEAL
                     AAAGVRYAQGYLFGRPQAPWAAVAWRPHGAEAPAGTVG"
     gene            4197..4622
                     /locus_tag="VNU01_04815"
     CDS             4197..4622
                     /locus_tag="VNU01_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31972.1"
                     /translation="MYRDDECAVCGESLPPDHLYCREHGAEVDDRLHDIGALLARLGE
                     DVPRLARLLDQVAGETYDWLAEAHEPEADWPPAPPLALRVDPEDVDVDLDSEPGYVRV
                     AIRPRLADLLGALDAALREAGAGAFAEACARAEGANATH"
     gene            4658..5620
                     /locus_tag="VNU01_04820"
     CDS             4658..5620
                     /locus_tag="VNU01_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HWH31973.1"
                     /translation="MHYRKLGRWGVKVSAVGLGSWLTYGNDVDERTAADCIRRAYELG
                     VTFFDTANVYARGRAEEVMGPVVREFPRSDYVLATKVFFPMGEGPNDSGLSRKHVFEQ
                     CEASLRRLGLDYVDLYQCHRYDKRTPLEETCRAMDDLIRQGKILYWGVSEWSADQIAH
                     AVDLCRANGWAPPVSNQPQYSALWRGIERRVVPTSAEHGVGQVVWSPLAMGILTGKYT
                     SVDQVPSDSRAASSGSAFMQRYFTQDVLDAVQRLKPLADEAGCSLAQLALAWCLRLDD
                     VSSVIVGATKVSHVDDNVAAADLALSGDVVAEMDRILEPVVADR"
     gene            5617..6087
                     /locus_tag="VNU01_04825"
     CDS             5617..6087
                     /locus_tag="VNU01_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWH31974.1"
                     /translation="MSSPTAPTSQSGREPLTARRALPRFALVGAIGVAVNESILHALH
                     GLAGLPLVPASVIATESAILGNYLGNELFTFHLRRLHLGRLLRFNAVALGGLVLTVGT
                     LWLLQHLTPWHYLVDNLVAIAVGSAWNFAANFRWTWGPAARLRGAARPGDEEAS"
     gene            6087..7055
                     /locus_tag="VNU01_04830"
     CDS             6087..7055
                     /locus_tag="VNU01_04830"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWH31975.1"
                     /translation="MPVAVVTGASGFLGRAFVHELEGRGYEVRGVDVRPGPKVTVGDV
                     SRPGRWTGVLDGADLVVHAAAIVAETGDLRAFWRVNVEGTRVVLDRAGAAGVGRVLHL
                     SSIVVHGARFPDGVDETGPVRMTGNPYTDTKVAAEHQALLAAASGRTAVTIVRPGDVY
                     GPHSVPWTLRPVELMKSGVFVLVDGGKGILSPVYVDDVVAGALTAADHPDAAGHVFHL
                     TGGVGVTAREFFGHYAQWLGIGLRSLPRPAAAALVAPLELVSRGLGRTTPVSVRAFEY
                     VSHPGTYSIDKARDMLGWEPRVSLEDGMARTREWLEEVGLVTPPPT"
BASE COUNT          875 a         2684 c         2707 g          938 t
ORIGIN      
        1 tcgccccaca gcacgtcggt caggtagggg aacgcgccgg cgtcccagtc cacgcgcgtg
       61 cgggagctga ccgaccccga ctgggtggag ctcgtgggcg gcgtgccgcg ctggctgccg
      121 gacgggcggc tgctgcacac cgtcgacgag gaggacacga ggcgcctggc ggccgacggc
      181 gtggcgctca ccccgccggg cctgcaggtg cgccgggtcg ccggcgtgca ggacggccac
      241 gtcgtgttca ccgcctcacg cgagccgacg gccaccgagc tgtaccgggt ggcgctggcc
      301 ggcggcgacc cggagccgct gaccgcgccc ggcggcatgg ccgacggcgc ggtgggcggc
      361 ccgtcgctgg tcaccgtgcg ccggtcgctg gagcgctccg ggccggaggt ggtggtgcgc
      421 cgcggcggcg tggcgcgcgc ggtggcgggc gcggccctgc cgcacgggtt ggagctgcac
      481 gtgcggctgc tggagctggg gccccgcgcc ctgcgcgccg cgctggtgct gccggccggg
      541 cacggcccgg gcgacggccc gctgccggtg ctgctggacc cctacggcgg gccgcacgcg
      601 cagcgcgtgg tgcaggccgc cgacgcctac ctcacctcgc agtggttcgc cgaccagggc
      661 ttcgcggtgc tcgtcgccga cgggcgcggc acgcccgcgc gcggcccggc ctgggagaag
      721 gccgtggcgg gcgacctcgc gagcgcgccg ctggaggacc aggtcgacgc gctgcacgcc
      781 gccgccgagg ccgtgcccgg cgcgctggac ctgtcgcggg tggccatccg cggctggtcc
      841 ttcggcgggt tcctggcggc gctggccgtg ctgcgccgcc cggacgtgtt ccacgccgcg
      901 atcgccgggg cccccgtgac ggactggtcc ctgtacgaca cgcactacac cgagcgctac
      961 ctgggcacgc cgcaggacga cgcggcgcgg taccggcgca gctcgctgct cgacgacgcc
     1021 gccgggctgg cgcggccgct gctgctgatc cacggcctgg cagacgacaa cgtcgtggcg
     1081 gcgcacacgc tgcgcctgtc cggcgcgctg ctggccgcgg ggcggcccca cgaggtcctg
     1141 ccgctgtccg gcgtcacgca catgacgccc caggaggtcg tggcggagaa cctgctgcgc
     1201 ctgcagctgg agttcctgcg gcgccatctc gaacgccccc ggacctgagt tcgggcagga
     1261 aggccgagat gccggttgag agatccaacc cgcccgcact ctcgtgacaa cttcgcacgc
     1321 gcgtgcagat gacggcggcg acgtcacccg gctggcccgt ctgcggggca cgtacacgcc
     1381 cggcacggtg ctgatcggcc cgtcaggtat cggcaagtcg gtgctggtga accgcttcgc
     1441 ggcccgatcg gccgccgagg gcgacgtggt gctcgacgcg gttcgggtcg cccggcgctc
     1501 ggaccctgtc gcgcaactgg ccggggtggt cagggccgca gccggccaga tcgcctccgg
     1561 acggcgacac gggacactcg gcgagctgat ggaccgggtg cagctgatcg cgatcaaggg
     1621 cgtgcagttc gcagttcggg agggagtcgg caacccgcac ctgtcggtcc gtgacgcgtt
     1681 ggtgcatctc ggggaagtcc tcgtccggga gaacgtcacc cgccggaagc cgcgggcgct
     1741 gctggtccgc ctggacgaac tgcagaacgc cgacgacacc cagcgttccg cactgctgac
     1801 cgcgatcggc gacctgctcg agcacacggt cgaactcgat ctgccgggcg ggcggctgcc
     1861 catgcacctg ccggtgctgg tgtacgtcac cggcttgcca gacctgctca accgagccac
     1921 caacgtcgac accttccggc gccggttcga cacccgcaag ctgacgctgt tcgtcgatca
     1981 ggaggtgacg agttcgctgc tgggcgccca actgccggac agggtgcggt tcaccgacga
     2041 ggcggccgcc cggctggccg agttggtgag cggcgacccg tacctgtgcc agtacgtcgg
     2101 ccaggccgcc tggaacggac gccgccgcag atcgcgaccg ccgcccgacg gctcgaacag
     2161 cgcgcgctga tcctgcgtga gcgcggcggc tggcgggtga cacaccgact ggtccgcaga
     2221 ctgcgcgaga ccggcgacat cgcctgatca gcgtgcgcga cgctcgaagt accgcctgca
     2281 gcccccacat caccaggccg tcgaactcgc caggacctga ggccggcgtg ctcgctacgc
     2341 tgcacagggc acagggcggc acggcgtggg gagcgaccgg ggatgagggc gctggaggcg
     2401 ggcgagcacg agctcgcgtt ctggcagcgg cacgtgcgca tcggcgtgtc gctgagccag
     2461 ctgacactgg gcgtgtgcct ggtgtacacg ctgcgctacg ccgacccgtc ccgccgcggc
     2521 gtgctgctgg cgctcatcgg catcgcgttc gcctccaccc ttggcgtcaa cctgctgccg
     2581 ctgcgccgca tcctgtccgg ccgcgccgcc gtgccgttct tcgcggcctg gtcggccctg
     2641 ctgaccctgc tcatcgtgct gggctccctg ggcgacggcg gcggcgacac ccccctggcg
     2701 ctgctgtact tcatgccggt ggtgtacggc gcgatcgcct acccgcccgg cggcgtgatc
     2761 gccctgggca tcgcggtggt cggcgccgcc gttgtcacgc gcatcgtcgg cggaggatcc
     2821 cccggcggca cgctgatgct cggcggcacg ctggccctgg tcaccaccat gtgcgcggtg
     2881 agcgcccgca accactggcg caccacgcgc agccagaccg ccctggccga gcgcctggcg
     2941 cacctggccg accacgacgg gctcaccggc tgcctcaacc accgcgcgtt ccacgagcgc
     3001 ctgcgccagg aggccgcgcg cgcggtgcgc tacgagcgcg ccctcggcct gctgctggtg
     3061 gacctcgacg gcttcaaggc ggtcaacgac gggcacggcc acccgtgcgg cgacgccgtg
     3121 ctggcggccg tcggcgacgt gctgcgggcc accacgcgcg acaccgacat cgtgggccgg
     3181 gtcggcggcg acgagttcgc cgtgctgctg cccgagaccg ggtgcgacga ggcgcacctg
     3241 ctcgccgagc gcatccgcgc cgccgtcggc gccctgcgcg agcccgtgcc ggtcaccgcg
     3301 tccatcggcg tggcggcgct gccgccggcg cacgacgccg cgaccctgct ggcggccgcc
     3361 gacgtggccg tgtaccaggc gaagcgctcc ggccgcgact acgtcgtcgt ccacggcgac
     3421 cagccggccg cccggcagca gggcggctcc cacgcgctgc tgcgcgagcg cgtccacgag
     3481 gtcgtcgacg gcgggcgcct gaccgcgctg ttccagccga tcgtgtgcct ggccgacggc
     3541 tcggtgctgg gctacgaggc gctcacccgc gtggacgagt ccagcctggg gccggcgcag
     3601 tggctggacc tggccgagcg ggccggcgtc cgcgacagcc tggaggcagc gatgtggcgg
     3661 actgcgctcg cagccggccc gccgccggac gccgccgtgc tgttcctcaa cgccggcccg
     3721 gaggcgctgc tgtccggcgc catggccgag ttccgcgggc gcctggggcc ccacgccgcc
     3781 atcgaggtga gcgagcacga cgccatcgcg gactacggcc tgctgagcgg cgccctggcg
     3841 ccgtggatcg gcgacggcgc ccgcatcgcc gtggacgaca tgggcgccgg ccacgcgaac
     3901 ctcgcccacg tgctgaacct gggcccgcac tacctgaagc tggaccgcag cctggtggag
     3961 ggcctccacg agcacccgtc ccgccgagcg ctgctggagt cgctgatcac gttcgccgcc
     4021 cgcatcggca gcgagctggt cgccgaaggc gtcgagctgg agcaggaggc cgaggcgctg
     4081 gccgccgccg gggtgcgcta cgcgcagggc tacctgttcg gacggccgca ggcgccctgg
     4141 gccgcggtcg cctggcggcc gcacggcgcc gaggcgcccg ccggtacggt gggctgatgt
     4201 accgagacga cgaatgcgcg gtgtgcgggg agtccctgcc gccggaccac ctgtactgcc
     4261 gcgagcacgg cgcggaggtc gacgaccgcc tgcacgacat cggcgcgctg ctggcccgcc
     4321 tcggcgagga cgtcccccgc ctcgcgcggc tgctggacca ggtcgcgggc gagacctacg
     4381 actggctcgc ggaggcccac gagccggagg ccgactggcc gcccgcgccg ccgctggcgc
     4441 tgcgcgtcga cccggaggac gtcgacgtgg acctggacag cgagccgggc tacgtgcgcg
     4501 tcgcgatccg cccccggctg gccgacctgc tcggcgccct ggacgccgcg ctgcgcgaag
     4561 ccggcgcggg cgcgttcgcg gaggcgtgcg cgcgggcaga gggcgccaac gcgacccact
     4621 aggatcgccg ctcaccgccg catcaaggag cgcacgcatg cactaccgca agctcggccg
     4681 ctggggcgtc aaggtctccg cggtgggtct gggatcgtgg ctgacctacg gcaacgacgt
     4741 cgacgagcgc accgccgcgg actgcatccg gcgggcctac gagctgggcg tcaccttctt
     4801 cgacacggcc aacgtctacg cgcggggccg cgccgaggag gtcatggggc cggtggtgcg
     4861 ggagttcccg cgctccgact acgtgctggc caccaaggtg ttcttcccca tgggcgaggg
     4921 gcccaacgac tcgggcctgt cccgcaagca cgtcttcgag cagtgcgagg cctcgctgcg
     4981 ccggctcggg ctggactacg tggacctgta ccagtgccac cgctacgaca agcgcacgcc
     5041 cctggaggag acctgccgcg cgatggacga cctcatccgg cagggcaaga tcctgtactg
     5101 gggcgtcagc gagtggtccg ccgaccagat cgcgcacgcg gtggacctgt gccgcgccaa
     5161 cggctgggcg cccccggtca gcaaccagcc gcagtactcg gcgctgtggc gcggcatcga
     5221 gcgccgcgtc gtccccacgt ccgccgagca cggcgtcggg caggtggtct ggtcgccgct
     5281 ggcaatgggc atcctcaccg gcaagtacac gtcggtggac caggtgccct ccgacagccg
     5341 cgccgccagt tccggcagcg cgttcatgca gcgctacttc acccaggacg tgctcgacgc
     5401 ggtgcagcgg ctcaagccgc tggccgacga ggccgggtgc tccctggcgc agctcgccct
     5461 ggcgtggtgc ctgcggctgg acgacgtctc cagcgtgatc gtaggcgcca ccaaggtctc
     5521 ccacgtcgac gacaacgtcg cggccgcgga cctggcgctg agcggcgacg tcgtcgcgga
     5581 gatggaccgc atcctggaac ccgtggtcgc cgaccgttga gcagccccac cgcaccgacg
     5641 tcgcagtccg gccgtgagcc gctcacggcc cggcgcgcgc tgccgcgctt cgcgctggtg
     5701 ggggcgatcg gcgtggccgt caacgagtcg atcctgcacg ccctgcacgg gctggcgggc
     5761 ctgccgctgg tgccggccag cgtgatcgcc acggagtcgg ccatcctggg caactacctg
     5821 ggcaacgagc tgttcacctt ccacctgcgc cggctgcacc tgggccggct gctgcgcttc
     5881 aacgcggtgg cgctgggcgg gctggtgctc accgtcggga cactgtggct gctgcagcac
     5941 ctgacgccgt ggcactacct cgtcgacaac ctggtggcca tcgcggtggg gtcggcgtgg
     6001 aacttcgcgg cgaacttccg ctggacgtgg gggccggcgg cgcggctgcg cggcgcggcc
     6061 cgccccgggg acgaggaggc gagctgatgc ccgtggccgt cgtgaccggc gcctccggct
     6121 tcctggggcg cgccttcgtc cacgagctcg agggccgcgg ctacgaggtc cgcggcgtgg
     6181 acgtgcgccc cgggccgaag gtcaccgtgg gcgacgtgag ccggcccggc cgctggaccg
     6241 gcgtgctgga cggcgcggac ctcgtggtgc acgccgccgc catcgtggcc gagaccggcg
     6301 acctgcgcgc cttctggcgc gtcaacgtgg agggcacgcg cgtcgtgctc gaccgcgcgg
     6361 gcgcggccgg ggtgggccgc gtcctgcacc tgtcctccat cgtcgtgcac ggcgcgcgct
     6421 tcccggacgg cgtcgacgag accggccccg tgcgcatgac cggcaacccg tacaccgaca
     6481 cgaaagtcgc cgcggagcac caggcgctgc tggccgccgc atccggccgc acggccgtga
     6541 ccatcgtgcg cccgggcgac gtctacgggc cgcacagcgt gccgtggacg ctgcggccgg
     6601 tggagctgat gaagtccggc gtgttcgtgc tggtcgacgg cggcaaggga atcctgtcgc
     6661 cggtgtacgt cgacgacgtg gtggccgggg cgctcaccgc ggcggaccac ccggacgccg
     6721 ccggccacgt cttccacctc accggcggcg tcggcgtgac cgcgcgggag ttcttcggcc
     6781 actacgcgca gtggctcggc atcggcctgc gctcgctgcc ccggccggcc gccgccgcgc
     6841 tggtggcgcc gctggagctg gtgtcccggg ggctgggccg cacgaccccg gtgtccgtgc
     6901 gcgccttcga gtacgtcagc cacccgggca cctactcgat cgacaaggcg cgcgacatgc
     6961 tgggctggga gccgcgggtg tcgctggagg acggcatggc gcgcacccgc gagtggctgg
     7021 aggaagtggg gctcgtcact cccccgccca cctgacgcgg ggggcgcacc acgctgcggc
     7081 ggcgcgcagc gcgtgggcca cgccgtcgtc ggccagctcc ggcaccacca ggtgggcggc
     7141 cgcccgcacc tgcgccggcg cctgccccat cgcgatggcc gtgcccgcga cgcgcagcat
     7201 cgac
//
LOCUS       DATMKC010000162         6686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_339516, whole genome shotgun sequence.
ACCESSION   DATMKC010000162 DATMKC010000000
VERSION     DATMKC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339516"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VNU01_04835"
     CDS             complement(<1..271)
                     /locus_tag="VNU01_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWH31976.1"
                     /translation="MSEAFSFEPPEQPVPIRDAATVIVLRQGERGAEALLLRRHGRSG
                     FAADAWVFPGGVLDPADSALPPAQWRGIDPGELAPRFHAEPARTLG"
     gene            324..977
                     /locus_tag="VNU01_04840"
     CDS             324..977
                     /locus_tag="VNU01_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWH31977.1"
                     /translation="MDETPAGAPSERARVRQHADRARYGRDEVDAVLDAGVVAHVGFL
                     REGCPVVVPMAYGRVGDTLYLHGGVASRLLAALGDGAEACVTVTLVDGLVLARSAFKH
                     SMNYRSVVAHGRGRAVTDLEEKAAGLRAVTSHNVAGRWDELRPPTRKELAATAVVAFP
                     LTECALKVREGGPLDHTEDLDQPVWAGVVPLRLVAGDPVPAAGAADRPPPRPRPGMR"
     gene            1069..2232
                     /locus_tag="VNU01_04845"
     CDS             1069..2232
                     /locus_tag="VNU01_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH31978.1"
                     /translation="MDFSIPEELLSLRESFRAFLDREVRPVEDRFKAELDEGRFGPEL
                     REGGDAIKRRSLAEGFYAAHMPEEVGGWGLSNLGMTLLVEEAARSGMRMALFALGPPN
                     PEAPTPLLMDLPEHLRDTYIKPLMAGETSMCFALTEPEAGSDAQSIRTRAVRDGDSWV
                     LNGMKHYITNGDRADFAVVFAVTDAEKRAHGGITAFIVPREAYSVGKKQYTIADTHPV
                     ELVFEDSRIPADHIIGQEGFGFYAAMKFLNGGRAYIGAQCLGLAQWCLDVAVEHAKTR
                     TAFGKPIGKFQGVSFPLAESKVEIEAMRWLVYHLAWSVDQGEQPMLDSSIVKYFATDR
                     AYQIADRCLQAFGGQGLLKEGRIEAALRHTRMLRVVEGASEVQKLVISRSLGL"
     gene            complement(2253..3482)
                     /locus_tag="VNU01_04850"
     CDS             complement(2253..3482)
                     /locus_tag="VNU01_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH31979.1"
                     /translation="METTVQAFENPYRPGAGQMPPHLAGRQKEYDEFDRLLRQQTILD
                     NLVLTGLRGVGKTVLLETFKPRAQRQGWLWVGTDLSESASVSETNLAIRLLADLAVVT
                     SGISIEARTSPGVGFGPAPEPTDVALTYQVLERVFQQTPGLISDRIKAVLEFVWAVVK
                     DHDRSRVIFSYDEAQNLSDHAARDEFPLSVLLDVFQSIQRKGIPFMLVLTGLPTLFPK
                     LVEARTFAERMFRVVMIDKLSDEESREAIIKPLEQQSCPVRLATPSVDAICRESGGYP
                     YFIQFICREVYDVIIQDIADQGSLLPVSLEAIQRKLDTDFFAGRWAKVTDRQRQLLYT
                     VATLGHGQDEFTIQELVEASRQLLEKPFSSSHASQMLATLGDYGIIYKNRFGKYSFAV
                     PLLDKFILRTYELPARV"
     gene            3638..4990
                     /gene="selA"
                     /locus_tag="VNU01_04855"
     CDS             3638..4990
                     /gene="selA"
                     /locus_tag="VNU01_04855"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HWH31980.1"
                     /translation="MNSPRNLPRIDDLLAEAAGLVARYGRGAAADALREVVDRTRAAL
                     LDGSPAATTADDLIAQAETLLAARRPSPPRKVINATGVVVHTNLGRAPLSAAAVAAIA
                     DAAGPCDLEYDLAGGRRGSRGDHVDALIAEACGAQDALAVNNAAGALVLVLAALAGPR
                     GVLVSRGELIEIGGSFRLPDIMAASGARLVEVGTTNRTRAGDYAAAADADADVALVLK
                     VHPSNYTIDGFAESPSVAELHAVTQPRELPLVHDVGSGLLHPDARPWLADEPSMRGSL
                     DAGADLVLCSGDKLLGGPQAGLLAGRGDLIRACRSHPLARALRLDKLRIAALHATLEA
                     HLREPEAELPVWRMLAAAGESLRERAARLAAAVGPQADVCEGESVVGGGSAPGARIPG
                     PVLRLAVASAERAARMLREGDPPVVVRVEDGAVWVDPRTVEEADDALLAARLREAAGA
                     "
     gene            4987..>6686
                     /locus_tag="VNU01_04860"
     CDS             4987..>6686
                     /locus_tag="VNU01_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWH31981.1"
                     /translation="MSAAGWARSLARLAVVRRVRRLAADSRTAEPLLLALAVAVGLGT
                     GLLAVGLIEGVELVQRLAWGGDIPPWRVVAVPAAGALLVGVLVTYWMPEARGGGVGEV
                     MTSIALHGGRMRPGLALGKLATSALNLGTGASGGREGPITQIGGSVGSSIGRLLALDE
                     EQKRALIAAGAGAGIAASFNAPIGGMLFALEVILGGFRARYLQVVVVASVVASVTARE
                     IVGPELIYDPPPYRLGDPRELLLYAVLGLAAAAVGIAYIRGEDAVGIWVQRLRLWPPL
                     RLAVGGLGVGVIALVVPEVLGTGDRLPAVPGTMREPIADMLAGGGGGIAGDGAGAWTL
                     AGLLLVLLAAKLLATVLTLGTGNSAGSFSPAVFLGAALGGAFGHAAVTLLPASEVSPG
                     AFALVGMAGVLGASVRAPLTAILLAFELTGDYGMVLPLMLTTGIATMIADRLQPESLY
                     TLPLRRRGIVYGEPEDVDIMQTVQVAEIMTREPVTVPADLPLRELHERFRRTRLHGFP
                     VVDGDRLVGVVTLADLNRVAETTGDLMAQGGPGEGLRASDICTRNPLTVTPGDPVFRA
                     V"
BASE COUNT          910 a         2372 c         2435 g          969 t
ORIGIN      
        1 gcccaagggt gcgcgccggc tccgcgtgga agcgcggcgc cagctcgccg gggtcgatgc
       61 cgcgccactg cgccggcggc agcgcgctgt cggccggatc caggacaccc ccggggaaca
      121 cccacgcgtc ggcggcgaag ccgctgcggc cgtggcggcg cagcagcagc gcctcggcgc
      181 cgcgctcgcc ctgccgcagc acgatcacgg tcgcggcatc gcggatcggc acgggttgtt
      241 ccggcggctc gaacgagaag gcttccgaca tcgtggaggc atcctagagg ccaccagcgg
      301 ttgatcgagg cgggaggcgg gcgatggacg agacgccggc aggggcaccg agtgagcggg
      361 cacgcgtgcg ccagcacgcc gaccgtgcgc gctacgggcg cgacgaggtg gacgcggtcc
      421 tggacgcggg cgtggtcgcc cacgtcggct tcctccgcga aggctgtccg gtcgtggtgc
      481 ccatggcgta cgggcgggtg ggcgacaccc tctacctgca cggcggcgtg gccagccgcc
      541 tgctggccgc cctgggcgac ggcgcggagg cgtgcgtgac ggtcacgctg gtcgacggcc
      601 tggtgctcgc gcgctcggcc ttcaagcact cgatgaacta ccgcagcgtc gtggcccacg
      661 ggcgcgggcg cgccgtcacg gacctcgaag agaaggccgc cggcctgcgc gccgtgacca
      721 gtcacaacgt ggcgggccgc tgggacgagc tgcggccgcc gacgcgcaag gagctcgcgg
      781 cgacggccgt cgtggcgttc ccgctcaccg aatgcgcgct gaaggtccgc gagggtggtc
      841 cgctcgacca cacggaggac ctggaccagc cggtgtgggc cggtgtggtg ccgctgcgcc
      901 tggttgcggg cgatcccgtc cccgccgccg gagccgcgga tcgtccacca ccacgaccgc
      961 gccccggcat gaggtgacgg gccgctcacg gccggaacga ttctcgtaag atcgttcagc
     1021 gcggatccac caggtccccg cccgcccgag cacaggagca tcgtcgcgat ggacttctcg
     1081 atccccgagg aactgctcag cctgcgggag agcttccgcg ccttcctgga ccgcgaggtg
     1141 cggccggtcg aggaccgctt caaggcggaa ctggacgagg gccgcttcgg gccggagctg
     1201 cgcgagggcg gcgacgccat caaacggcgc tcgcttgcgg agggcttcta cgccgcgcac
     1261 atgccggagg aggtcggcgg ctggggcctg tccaacctcg gcatgacgct gctggtggag
     1321 gaggcggccc gcagcggcat gcgcatggcg ctgttcgcgc tgggcccgcc gaaccccgag
     1381 gcgccgacgc cgctgctcat ggacctgccg gagcacctgc gcgacaccta catcaagccg
     1441 ctgatggccg gcgagacctc gatgtgcttc gcgctgacgg agccggaggc gggctccgac
     1501 gcccagtcca tccgcacgcg cgcggtgcgc gacggcgaca gctgggtgct caacggcatg
     1561 aagcactaca tcacgaacgg cgaccgcgcg gacttcgcgg tggtgttcgc ggtgacggac
     1621 gccgagaagc gcgcgcacgg cggcatcacc gcgttcatcg tgccgcgcga ggcctacagc
     1681 gtcggcaaga agcagtacac gatcgccgac acccacccgg tggagctggt gttcgaggac
     1741 agccgcatcc ccgccgacca catcatcggc caggagggct tcggcttcta cgcggccatg
     1801 aagttcctca acggcgggcg ggcctacatc ggcgcgcagt gcctgggcct ggcgcagtgg
     1861 tgcctggacg tcgcggtgga gcacgccaag acgcgcaccg cgttcggcaa gcccatcggc
     1921 aagttccagg gcgtcagctt cccgctggcg gagagcaagg tggagatcga ggcgatgcgc
     1981 tggctcgtct accacctggc gtggtcggtg gaccagggcg agcagccgat gctggactcc
     2041 tcgatcgtca agtacttcgc gaccgatcgc gcctaccaga tcgccgaccg ctgcctgcag
     2101 gcgttcggcg gccaggggct gctgaaggaa ggccgcatcg aggcggcgct gcgccacacc
     2161 cgcatgctgc gcgtggtcga gggcgcctcc gaggtccaga agctggtgat cagccggtca
     2221 ctgggcctct agcaccagtt gccccgcaga cctcacacgc gggcaggcaa ctcgtacgtc
     2281 cgcaggatga acttgtcgag caacggcaca gcgaacgagt acttgccgaa gcggttcttg
     2341 tagatgatcc cgtaatcgcc caacgtggcg agcatctggc tggcgtggct ggacgagaac
     2401 ggtttctcga gaagctgacg cgaagcttcc accagttcct ggatggtgaa ctcgtcctga
     2461 ccgtgcccga gggtggccac cgtgtagagg agttggcgct gacggtctgt gaccttcgcc
     2521 catcgtccgg cgaagaagtc ggtatccagt ttccgctgaa tggcttccaa ggagacgggc
     2581 agcagcgacc cttggtcggc gatgtcttgg atgatgacgt catacacctc gcgacagatg
     2641 aactggatga agtaggggta tccacccgac tcacggcaga tcgcgtccac gctgggcgtt
     2701 gcgagcctca ctgggcagga ctgctgctcc aatggcttga tgatcgcctc gcgcgactcc
     2761 tcatcgctga gcttgtcgat catcaccacg cggaacatgc gctcagcgaa cgtgcgggct
     2821 tcgacgagct tcgggaagag ggtgggcaac ccagtcagca ccagcatgaa ggggatcccc
     2881 ttccgctgga tcgactggaa cacgtcgaga agtaccgaga gggggaactc gtctcgggca
     2941 gcatggtccg aaaggttctg cgcctcgtcg taggagaaga tcacccggct acggtcatgg
     3001 tccttgacca ctgcccacac aaactcgagg actgccttga tgcggtctga gatcagcccg
     3061 ggtgtctgtt ggaaaacgcg ctccagcacc tggtaggtca acgcgacgtc ggtgggttcg
     3121 ggagcgggtc cgaagccaac ccctggcgag gtcctggctt cgatggaaat cccagacgtg
     3181 accaccgcca agtcggccag gagtcgaatg gcgaggttcg tctcgctcac gctggccgac
     3241 tccgacaggt cggtgcccac ccagagccac ccttgcctct gcgcccgtgg tttgaacgtc
     3301 tcgaggagca cggtcttgcc tacaccgcgc aggcccgtga gtaccaggtt gtcgaggatc
     3361 gtttgttgac gcagcaagcg atcgaactcg tcgtactcct tttgacgccc ggcgaggtgg
     3421 ggcggcatct gcccggcccc tgggcggtat gggttctcga aggcctgcac cgtggtttcc
     3481 atagctaatc cgcctccggg ctacatttag ctgctaatct aaaattagtc ggtcgtgtga
     3541 attcctgtca agctgcttgt ccacaccttg cggcccgcat accagcggtc ggctcgcaga
     3601 gcagccgggt gcccattggg ggtggcagga tgcatacgtg aactcgccca ggaacttgcc
     3661 gcggatcgac gacctcctcg cggaggcggc ggggctggtc gcgcggtacg ggcgcggcgc
     3721 ggccgccgac gcgctgcgcg aggtcgtcga ccggacacgc gcggcactgc tggacggcag
     3781 ccccgcggcc accacggcgg atgacctcat cgcgcaggcg gagacgctgc tggccgcgcg
     3841 ccggccgtcg ccgccgcgca aggtcatcaa cgccaccggc gtggtggtgc acaccaacct
     3901 cggccgcgcg ccgctgtccg ccgccgccgt ggccgccatc gccgacgcgg cgggcccctg
     3961 cgacctggag tacgacctgg ccggcgggcg ccggggctcg cgcggcgacc acgtcgacgc
     4021 cctcatcgcc gaggcgtgcg gcgcccagga cgccttggcc gtcaacaacg ccgccggcgc
     4081 tctcgtgctc gtcctcgccg ccctggccgg tccccgcggc gtgctggtca gccgcggtga
     4141 gctcatcgag atcggcggct ccttccgcct ccccgacatc atggcggcct ccggcgcgcg
     4201 cctcgtcgag gtgggcacca ccaaccgcac gcgcgcgggc gactacgccg ccgcggccga
     4261 cgccgatgcc gacgtcgcgc tggtgctgaa ggtccacccc tccaactaca cgatcgacgg
     4321 cttcgcggag tcgccgtccg tggccgagct ccacgccgtc acccagccgc gcgagctgcc
     4381 gctggtgcac gacgtcggca gcggcctgct gcacccggac gcgcggccgt ggctggccga
     4441 tgagccgagc atgcgcggct cgctggacgc gggcgccgac ctggtgctgt gcagcggcga
     4501 caagctcctg ggcggcccgc aggcgggact gctcgcgggc cggggggacc tcatccgcgc
     4561 gtgccgatcc cacccgctgg cgcgcgcgct gcgcctggac aagctccgca tcgccgcgct
     4621 gcacgccacg ctggaagcgc acctgcggga gcccgaggca gagctgccgg tgtggcgcat
     4681 gctcgcggcc gccggagagt ccctgcgcga gcgtgccgca cggctggccg ccgcggtcgg
     4741 cccgcaggcg gacgtgtgtg agggcgagtc cgtggtgggc ggcggctcgg cccccggggc
     4801 caggatcccc ggacccgtgc tgcgcctggc cgtggcatcc gccgagcgcg cggcgcgcat
     4861 gctgcgcgag ggcgatccgc cggtggtcgt gcgcgtggag gacggcgccg tctgggtcga
     4921 tccgcgcacc gtggaagagg ccgacgacgc cctcctggcc gcgcgcctgc gtgaggccgc
     4981 gggagcgtga gcgccgccgg ctgggcccga tcgcttgccc ggcttgccgt cgtgcgccgc
     5041 gtgcggcgcc tggcggccga ctcgcgcacg gcggagccgc tgctgctcgc gctcgccgtc
     5101 gccgtgggcc tgggcaccgg gctcctggcc gtgggcctca tcgaaggcgt cgagctggtc
     5161 cagcggctgg cctggggggg cgacatcccg ccctggcgcg tcgtggcggt gcccgcggcc
     5221 ggcgccctgc tggtgggcgt gctggtcacc tactggatgc ccgaggcgcg cggcggcggc
     5281 gtgggcgagg tgatgacctc catcgcgctg cacggcgggc gcatgcgccc cggcctggcg
     5341 ctgggcaagc tcgccacgtc ggccctcaac ctgggcaccg gtgcctccgg cggccgcgag
     5401 gggcccatca cccagatcgg cgggagcgtc ggctcgtcca tcggccggct gctcgcgctt
     5461 gacgaggagc agaagcgcgc gctcatcgcc gccggcgcgg gcgccggcat cgccgcctcg
     5521 ttcaacgccc cgatcggcgg gatgctgttc gcgctggagg tcatcctggg cggcttccgc
     5581 gcccgctacc tccaggtggt cgtggtcgcg tccgtggtgg cctccgtcac cgcgcgcgag
     5641 atcgtcgggc ccgagctgat ctacgacccg ccgccctacc gcctgggcga tccccgggag
     5701 cttctgctgt acgccgtgct cggcctggcc gcggccgccg tcggcatcgc ctacatccgc
     5761 ggtgaggacg ccgtgggcat ctgggtccag cggctgcgcc tgtggccgcc gctgcgcctg
     5821 gccgtcggcg ggctgggcgt cggcgtcatc gcgctggtgg tgccggaggt gctcggcacc
     5881 ggcgaccgcc tgcccgccgt gcccggcacg atgcgcgagc ccatcgccga catgctcgcc
     5941 ggcggcgggg gcggcatcgc gggagacggc gcgggcgcgt ggacgctggc cggcctgctc
     6001 ctggtgctgc tcgccgccaa gctcctggcc acggtcctca cgctgggcac cggcaactcc
     6061 gcgggctcgt tctcgccggc cgtgttcctg ggcgccgcgc tgggcggagc cttcggccac
     6121 gccgcggtga cgctgctgcc ggcgagcgag gtcagcccgg gcgcgttcgc gctggtgggc
     6181 atggccggcg tcctgggtgc cagcgtgcgc gcgccgctga ccgcgatcct gctcgcgttc
     6241 gagctcaccg gcgactacgg catggtgctg ccgctgatgc tcaccaccgg catcgccacg
     6301 atgatcgccg accgcctcca gcccgagtcg ctgtacacgc tgccgctgcg ccgccgcggg
     6361 atcgtgtacg gcgagccgga ggacgtcgac atcatgcaga cggtccaggt ggccgagatc
     6421 atgacgcgcg agccggtcac cgtgcccgcg gacctgccgc tgcgcgagct gcacgagcgc
     6481 ttccgccgca cgcgcctgca cggcttcccc gtcgtggatg gcgaccgcct ggtcggcgtg
     6541 gtgacgctgg ccgacctcaa ccgcgtggcc gagacgaccg gcgacctcat ggcccagggc
     6601 ggccccggcg agggactgcg cgcctcggac atctgcacgc gcaacccgct gaccgtgacc
     6661 cccggcgacc cggtgttccg cgcggt
//
LOCUS       DATMKC010000163         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_101097, whole genome shotgun sequence.
ACCESSION   DATMKC010000163 DATMKC010000000
VERSION     DATMKC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101097"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VNU01_04865"
     CDS             <1..1188
                     /locus_tag="VNU01_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31982.1"
                     /translation="CGGGAAGEPKDQLVEALRAMGQDAGVTVQVSLDTDEASLAALNA
                     AGDEPMPPELVALVSQAWMRVSLPFDPEAKTAEVSLGKAADQLLAFRVIGDDVYVQAD
                     VAALVDAAGQDPAVLDGARAMLTETGVLPPDVLDGRWVVLRGATQLAEELSGQEVANP
                     LDQMRAREAMQRLGRTLAEEAAITRVGQDAQGDHLRAVLPLRRLYSVFLEEAEAMGLP
                     TPSGEAPALEEVPDESVTVDVWVRDGRLSVVSLDLRQLAAMDPEAPEIPAEVERFALR
                     AEFTPFDGVVDVPADAVELNPEALMGLFMGAMGGAMGPLMGGATMEGMPPGFDPSAMP
                     PGMAPGAFDQPPPDMMAPEDHAMAGQEFYAQPGYAEARQDYCAQVADVPESQRRHYED
                     ICP"
     gene            complement(1236..2105)
                     /locus_tag="VNU01_04870"
     CDS             complement(1236..2105)
                     /locus_tag="VNU01_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH31983.1"
                     /translation="MEHTAHAAGGRQAAALARTLVRPRAYLGTARELAIGAFSLATYP
                     LGMRAPVAPAPLRYAPARAEQRSLMTLAPEVAWTPIVLVHGYFHNRSAFLAMSRSLRR
                     AGFQYVHTMNYNPLAADVPALAEQLREEVQRVLDATGAEQVQIVGHSMGGVVARTYVQ
                     MHGGDEHVDTVVSLGSPHRGTYSAHLGMGPAARQLRPGSAFLRTLESSARPGPVRWIS
                     YYSDLDAMVIPASSAKLVHPALKAVNVRTRDTGHLSLLVRGEVLRGLVGYLSDPWAGR
                     GGAPAAGADAAAG"
     gene            complement(2249..>2455)
                     /locus_tag="VNU01_04875"
     CDS             complement(2249..>2455)
                     /locus_tag="VNU01_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31984.1"
                     /translation="SEVAYCRASRRLESDAGTVWSVDGRHLGGPGRSLTPLPSQVHDG
                     TLYVNVTEPLTAPPAATVGAEPAC"
BASE COUNT          310 a          905 c          933 g          307 t
ORIGIN      
        1 tgcggaggtg gcgccgccgg cgagcccaag gaccagctcg tcgaggcgtt gcgcgcgatg
       61 ggccaggacg cgggcgtcac cgtgcaggtg tcgctggaca ccgacgaggc gtccctggcc
      121 gcgctcaacg ccgccggcga cgagccgatg ccgccggagc tcgtggcgct cgtcagccag
      181 gcgtggatgc gggtgtccct gccgttcgac ccggaggcca agaccgccga ggtctccctc
      241 ggcaaggccg cggaccagct gctggcgttc cgcgtcatcg gcgacgacgt ctacgtgcag
      301 gccgacgtgg cggccctcgt cgacgccgcg gggcaggacc cggccgtgct cgacggcgcc
      361 cgggcgatgc tgaccgagac cggtgtgctg ccgcccgacg tgctcgacgg ccgctgggtc
      421 gtcctgcgcg gcgccaccca gctcgccgag gagctgagcg gccaggaggt cgccaacccc
      481 ctcgaccaga tgcgcgcccg tgaggccatg cagcgcctgg ggcggacgct ggcggaggag
      541 gcggcgatca cgcgggtcgg ccaggacgcg cagggcgacc atctgcgcgc cgtgctgccg
      601 ctgcgccgcc tgtacagcgt gttcctggag gaggccgagg ccatggggct gcccacgccg
      661 tccggcgagg cgcccgcgct ggaggaggtg cccgacgagt ccgtcaccgt ggacgtgtgg
      721 gtgcgcgacg gccgcctcag cgtggtgtcg ctggacctgc gccagctcgc cgcgatggac
      781 cccgaggcgc cggagatccc ggccgaggtg gagcgcttcg ccctgcgcgc cgagttcacc
      841 cccttcgacg gcgtggtcga cgtgcccgcc gacgcggtgg aactgaaccc ggaggcgctc
      901 atgggcttgt tcatgggtgc catggggggc gcgatggggc cgctcatggg cggcgccacg
      961 atggagggca tgccgcccgg cttcgacccg agcgcgatgc cgcccggcat ggcgccgggc
     1021 gcgttcgatc agccgccgcc ggacatgatg gcgccggagg accacgcgat ggccggccag
     1081 gagttctacg cccagcccgg ctacgccgag gcccgccagg actactgcgc ccaggtggcc
     1141 gacgtgccgg agtcccagcg ccgccactac gaggacatct gtccgtagcg gtccgtaccc
     1201 ggctgacgcc gctgccccgg tgcgcggggg aacgctcagc cggcggcggc gtcggcgccc
     1261 gcggccggtg ccccgccgcg tccggcccag gggtcggaca ggtagccgac caggccgcgc
     1321 agcacctcgc cgcgcaccag cagcgacagg tgcccggtgt cgcgcgtccg cacgttcacg
     1381 gctttgaggg cagggtggac cagcttggcg ctggacgccg ggatcaccat ggcgtccaga
     1441 tcgctgtagt aggagatcca gcgcaccggc ccgggccgcg cactggactc cagggtgcgc
     1501 aggaacgccg agcccggccg cagctggcgc gcggccggcc ccatgcccag gtgcgccgag
     1561 taggtgcccc ggtgcggtga gcccaggctg accaccgtgt cgacgtgctc gtcgccgccg
     1621 tgcatctgca cgtacgtgcg cgccacgacg ccgcccatcg agtgcccgac gatctgcacc
     1681 tgctcggcgc cggtcgcgtc cagcacgcgc tggacctcct cgcggagctg ctcggccaga
     1741 gccggcacgt ccgcggcgag cgggttgtag ttcatggtgt ggacgtactg gaacccggcg
     1801 cgccgcagcg accggctcat cgccaggaac gcgctgcggt tgtggaagta gccgtgcacc
     1861 agcacgatcg gcgtccaggc gacctcaggc gccagcgtca tcagtgagcg ctgctccgcc
     1921 cgtgccggcg cgtagcgcag cggggcgggc gccaccgggg cgcgcatgcc cagcgggtac
     1981 gtggccagcg agaacgcgcc gatggccagc tcgcgggcgg tgcccaggta cgcgcggggg
     2041 cggaccagcg tgcgagccag cgcggcggcc tggcggccgc ccgctgcgtg cgccgtgtgc
     2101 tccatccggt gccctcgcgc ggttcgctcg tggcccccgt ggtcgagtga tccggtcggc
     2161 cggtcgagcg cagaacttca ctgttgtagt gacaaattga cccgttccgt ccagccgcaa
     2221 acgcgcgccg ccagcggccg gcaggccatc agcaggcggg ctcggcgccc accgtggcgg
     2281 ccggcggcgc cgtcagcggc tcggtcacgt tgacgtacag cgtcccgtcg tgcacctggc
     2341 tgggcagcgg cgtcagggac cggcccggcc cgcccaggtg ccgcccgtcg accgaccaca
     2401 ccgtcccggc gtcgctctcc agccgccgcg acgcgcggca gtaggcgacc tcgct
//
LOCUS       DATMKC010000164         4244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_190682, whole genome shotgun sequence.
ACCESSION   DATMKC010000164 DATMKC010000000
VERSION     DATMKC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190682"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VNU01_04880"
     CDS             complement(<1..1411)
                     /locus_tag="VNU01_04880"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWH31985.1"
                     /translation="MNEVTLIAAETFCRCAPSGQIHPGGEQGLYVRDTRAVAGLVVRL
                     NGEEPHSLRGRAVSPAAGRFTAWWRDPRDTEPDPKLLLERRRVLSGSLWEQLRLTNHG
                     VEPVTASVTIAVVADFAYIFDVKHGRAGTPFPGEATGDGLAFRHPQTAHQVELRCDPA
                     PDGIDGAGGELRWEVALEPQRPWSLEVSVGFRDRAHEPVWPTRTWDADITTRGARPGP
                     SRWWTVPALRCTDPRLPALAAQAAADLESLLVADPVAPEDRFLAAGSPWFLTLFGRDS
                     IWSALMALPLDVSLAGQTLRVLARRQGTRVDPETEEQPGKILHEVRHGGLFERSDLPP
                     VYFGTMDATPLFVTLLHEAWRWGLPEEEVRALLPAAERALAWMRDHGDPDGDGFLEYR
                     RSGGRGLDNQGWKDSSDGIQFADGRLADAPIALCEVQGYAYDAAVRGAELLDAFGRAG
                     GAGWRQWAAALRERFRERFW"
     gene            complement(1408..2730)
                     /locus_tag="VNU01_04885"
     CDS             complement(1408..2730)
                     /locus_tag="VNU01_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWH31986.1"
                     /translation="MTETAPREQAGAPAQPAPGWPATLADCVHCGFCLPACPTYDLWG
                     QEMDSPRGRIWLMGAVAAGQPVDATFVRHVDACLGCMACVTACPSGVRYGELVETARA
                     HIEQARARPFTERVLRGLLFAVLPHPRRLRLIARLAWVAQRLGLRALAHRLGIVRRLP
                     RRLRALEALLPRKAPGRGGGAHEAAPRGEARLRVGLLTGCVQSVWFADVNTAAERVLV
                     AEGCAVTTPGPLCCGALELHSGREHQALRRARAVIAAFEERGADVVAVTAAGCGSAMK
                     DYGRLLAGDPEWAERAAAFAARVRDVTELLAELEPRAPRGPVRARVAYQDACHLGHAQ
                     GVRAQPRALLEAIPGVELVEPAEQGTCCGSAGIHNVLEPDTAAVLGARKASAVSAAEP
                     DVVATANPGCALQLARHLGPGVRVVHPVQLLDEALHPAARTGNKRGQP"
     gene            3003..4220
                     /locus_tag="VNU01_04890"
     CDS             3003..4220
                     /locus_tag="VNU01_04890"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWH31987.1"
                     /translation="MLYGGVDWAAEEHAISVLDAATEKFHRTVIKHTAEGFDQLVAWL
                     GRFGDPAELPVAIERPDGRLVDRLLEAGHPVVPVKTNAIKAWRESETISGAKTDPGDA
                     DVIADYLRVRHRGLRVLTPFSDETRALRLVVRTRDDLVAQRVAATNQLAALLDAFWPG
                     AKGVFADVESPIALAFLTRYPTPASAARLGEKRMAAFCAKHGYSGRRSPAELLKRLRG
                     APPGVTDPAQTEAGRDAVLALVAVLTGLNTALKDLDRSVVAHLGEHPDAEIFTSLPRS
                     GRINAAQMLAEWGDCRQAYDSAEAVSALAGQTPVTKKSGKHESVHFRWACNKRFRNAI
                     DTFADNSRHASPWAARVYADAIARGHDHPHAIRILGRAWIRVIHRCWTTRTPYDEALH
                     GGAQRLNAPLIAA"
BASE COUNT          576 a         1671 c         1485 g          512 t
ORIGIN      
        1 cccagaagcg ttcgcggaag cgctcgcgca gggccgccgc ccactgccgc cagccggcgc
       61 cgcccgcccg cccgaacgcg tcgagcagct cggccccccg caccgccgcg tcgtaggcgt
      121 agccctgcac ctcgcacagg gcgatcggcg cgtccgccag gcgcccgtcg gcgaactgga
      181 tgccgtcgct gctgtccttc cagccctggt tgtccaggcc gcgcccaccg gagcgccggt
      241 actccaggaa gccgtcgccg tccgggtccc cgtggtcgcg catccacgcc agtgcccgct
      301 ccgcggcagg cagcagcgcg cgcacctcct cctcgggcag cccccaccgc catgcctcgt
      361 gcagcagcgt gacgaacagc ggcgtcgcgt ccatcgtgcc gaagtacacg ggtggcaggt
      421 cgctgcgctc gaacaggccg ccgtggcgca cctcgtgcag gatcttgccc ggctgctcct
      481 ccgtttccgg gtccacgcgg gtcccctggc gccgcgccag gacacgcaag gtctggccgg
      541 ccagcgacac gtccagcggc agggccatga gcgccgacca gatggagtcg cgtccgaaca
      601 gggtcaaaaa ccaggggctg ccggccgcca ggaagcggtc ctccggcgcc acggggtcgg
      661 ccaccagcag ggactccagg tccgcggcgg cctgcgccgc cagcgcgggc aggcgcgggt
      721 cggtgcaccg cagcgccggg accgtccacc accgggacgg ccccgggcgc gcgccccgcg
      781 tggtgatgtc ggcgtcccag gtcctcgtgg gccagacggg ctcgtgggcg cggtcgcgga
      841 agcccacgga gacctccagc gaccaggggc gctgcggctc cagcgccacc tcccagcgca
      901 gttccccgcc cgcgccgtcg atcccgtccg gggctgggtc gcagcgcagc tccacctggt
      961 gggccgtctg cgggtgccgg aacgccaggc cgtcgccggt cgcctcgccg ggaaacggtg
     1021 tgcccgcccg gccgtgcttg acgtcgaaga tgtaggcgaa gtccgcgacc accgcgatcg
     1081 tcacggacgc ggtgaccggc tcgaccccgt ggttggtcag ccgcaactgc tcccacaacg
     1141 agccggacag gacccggcgc cgttccagca ggagcttcgg gtccggctcc gtgtcgcgcg
     1201 ggtcgcgcca ccatgcggtg aagcgcccgg ccgccgggct caccgcccgg ccgcgcagcg
     1261 agtggggctc ttcgccgttc agccgcacga ccagaccggc gacggctcgt gtgtcgcgca
     1321 cgtacaggcc ctgctcgccg ccgggatgga tctgcccgct gggggcgcac cggcagaacg
     1381 tctcggcggc gatgagcgtg acctcgttca tggctgccca cgcttgttcc ctgtgcgcgc
     1441 ggcgggatgc aacgcctcgt ccagcagttg caccgggtgc accacgcgca cgcccgggcc
     1501 gaggtgccgg gcgagctgga gcgcgcagcc ggggttggcc gtggccacca cgtccggctc
     1561 ggccgccgac acggccgagg ccttgcgcgc gcccagcacc gcggcggtgt cgggctccag
     1621 gacgttgtgg atgccggccg agccgcagca ggtgccctgc tccgccggct cgaccagctc
     1681 gacccccggg atggcctcca gcagcgcgcg cggctgcgcc cgcacgccct gggcgtggcc
     1741 caggtggcag gcgtcctggt aggccacacg cgcgcggacg gggccgcgcg gcgcgcgcgg
     1801 ctccagttcc gccagcagct cggtcacgtc gcgcacgcgc gcggcgaacg ccgccgcgcg
     1861 ttccgcccac tccgggtcgc cggccaagag gcgcccgtag tccttcatcg ccgagccgca
     1921 gccggccgcg gtgaccgcca ccacgtcggc gccgcgctcc tcgaacgcgg cgatcacggc
     1981 gcgcgcccgc cgcagcgcct ggtgctcacg gccgctgtgc agctccaggg ccccgcagca
     2041 cagcgggccg ggtgtggtca ccgcgcagcc ctcggccacc agcacgcgct cggcggcggt
     2101 gttcacgtcg gcgaaccaca ccgactgcac gcagccggtg agcaggccca cgcgcaggcg
     2161 cgcctcgcct cgcggcgcgg cttcgtgcgc gccgccgccg cggccgggcg ccttgcgcgg
     2221 cagcagcgcc tccagggcgc gcagccgcct gggcaggcgc cgcacgatgc cgaggcggtg
     2281 ggccagcgcg cgcaggccca gccgctgcgc cacccacgcc agccgcgcga tcaggcgcag
     2341 ccggcgggga tgcggcagca cggcgaacag caggccgcgc agcacgcgct cggtgaacgg
     2401 ccgcgcgcgg gcttgctcga tgtgggcgcg cgccgtctcc accagctcgc cgtagcgcac
     2461 gccggagggg cacgccgtca cgcacgccat gcagcccagg cacgcgtcga cgtggcggac
     2521 gaaggtcgcg tcgaccggct gcccggccgc caccgctccc atgagccaga tccggccccg
     2581 gggcgagtcc atctcctggc cccacaggtc gtaggtcggg cacgcgggca ggcagaaacc
     2641 gcagtgcacg cagtcggcca gggtggccgg ccagccgggc gcgggctgcg ccggcgcgcc
     2701 ggcctgctcc cggggtgcgg tctcggtcac tggtgcaccc tctccatagc aggcgagatg
     2761 tggttgtctg ctcgagtagc tcggcaaggg ccggggtgac ggccgacgtc ggtggccctt
     2821 cgagggccag catgacaccg ccgcccccgg accttgccag gttcgacacc ggcaagagag
     2881 ggacgcgccg tagagcgccg gtcagtgacc accttgggtg agcctggctt gccgcagctg
     2941 ctccttcccc gtggtcttcc ccacggtgca cgcgtcagcg tgcgtggcaa ggaggtgcgg
     3001 ccatgttgta cggaggagtg gactgggcgg cagaggagca cgcgatcagc gtgctcgacg
     3061 ccgcgaccga gaagttccat cggacggtga tcaaacacac cgccgagggg ttcgatcagc
     3121 tcgtggcctg gctgggccgc ttcggtgacc cggctgagct gccggtcgcg atcgagcgtc
     3181 cggacgggcg actggtcgac cggctgctgg aggccggcca cccggtcgtg ccggtcaaga
     3241 ccaacgcgat caaggcgtgg cgggaaagcg agacgatctc gggcgccaag accgaccccg
     3301 gcgacgccga cgtgatcgcc gactacctgc gcgtgcgcca ccgcggcctg cgcgtgctca
     3361 ctcccttcag cgacgagacc cgcgccctgc ggctggtggt gcgcacccgc gacgacctgg
     3421 tcgcccagcg cgtcgccgcg accaaccagc tggccgcgct gctcgacgcg ttctggcccg
     3481 gcgccaaggg cgtgttcgcc gacgtcgagt cgccgatcgc gctggcgttt ttgacccgct
     3541 accccacacc agcctcggcc gcccgcctcg gggagaagcg catggccgcg ttctgcgcca
     3601 agcacggcta cagcggccgc agatcccccg ccgaactgct caagcgcctc cgcggcgcgc
     3661 cgccaggcgt caccgatccc gcccagaccg aagccggccg cgacgccgtc ctggccctcg
     3721 tcgccgtcct gaccggcctc aacaccgcgc tcaaggacct cgaccggtcg gtcgtcgccc
     3781 accttggtga gcacccggac gccgagatct tcacttccct gccacggtcc ggtcggatca
     3841 acgcggccca gatgctcgcc gagtggggcg actgccgaca agcctacgac tccgccgaag
     3901 ccgtgtccgc cctcgccgga caaacacccg tcaccaagaa atccggcaag cacgagtccg
     3961 tgcacttccg ctgggcctgc aacaaacgct tccgcaacgc catcgacacc ttcgccgaca
     4021 acagccgcca cgccagcccc tgggccgcgc gcgtctacgc cgacgcgatc gcgcgaggcc
     4081 acgaccaccc ccacgccatc cgcatcctcg gccgcgcctg gatccgtgtc atccaccgct
     4141 gctggaccac ccgcaccccc tacgacgaag ccctccacgg cggcgcccaa cgcctcaacg
     4201 cgccgctgat cgcggcatag ggttgacata gagggtgtca tgcg
//
LOCUS       DATMKC010000165         8883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_314729, whole genome shotgun sequence.
ACCESSION   DATMKC010000165 DATMKC010000000
VERSION     DATMKC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314729"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VNU01_04895"
     CDS             <1..630
                     /locus_tag="VNU01_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWH31988.1"
                     /translation="ETGMIVPLGDWVLEHAAAEVAAWQARFRRPIDLAVNVSARQLDR
                     AGWPAGVAAILERSGLPATNLHLELTETALFESGTSTVGAFRGLRAQGVHIGIDDFGT
                     GYSSMSYLRDFPVDFLKIDRAFVSELDSRSRESTAIVEAILALGRALGLRTVAEGVET
                     AAQAERLAALDCDLAQGYLLGRPMPAAEFEAVLAAQPTSLAGMRAYGTG"
     gene            644..916
                     /locus_tag="VNU01_04900"
     CDS             644..916
                     /locus_tag="VNU01_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWH31989.1"
                     /translation="MDTDSAAGATPTPYTAQHIKDALAQDPRVGEMTFEVTLAGGRAF
                     VAGQVPTEQRRDAVPDVVRSVAPDLEVVNEVTVVPCDEPVGQEVIR"
     gene            913..1662
                     /locus_tag="VNU01_04905"
     CDS             913..1662
                     /locus_tag="VNU01_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWH31990.1"
                     /translation="MIRIAATGDVHFGEDSGGTLRPHLEHLGDNADLFLIAGDLTNWG
                     TVAEAQVLAEELRDLPVPVVLVLGNHDYQSDEPDKIRQVLTDAGVVVLEGESATFDVR
                     GQKVGIAGTKGFGGGFAGACGTAFGEPEMKAFIDHTQRLADRLERCLTDLDADHKVAL
                     MHFSPVEQTLRGERLEIYPFLGSYLLAEACDRGGADLIVHGHAHAGTEKGVTAGGVHV
                     RNASRFVIRHAYKVYCLGGAGDVACDDSAAA"
     gene            1670..2245
                     /locus_tag="VNU01_04910"
     CDS             1670..2245
                     /locus_tag="VNU01_04910"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HWH31991.1"
                     /translation="MATQDELRESMKKAAAALRDADVPFILAGSMACWARGGPVSQHD
                     VDFMVKPEDADRAVKALEEAGLRIERPPEGWLFKAWDGDVLVDLIWSPSGMEITDEVF
                     ARADALNVHAVTMRVMRLEDVIVTKLAALREHELDYERILEISRSLRELIVWDEVRRE
                     APDNPYVRAFFTMIEGLGIAPPSEAHTAATP"
     gene            2311..3192
                     /locus_tag="VNU01_04915"
     CDS             2311..3192
                     /locus_tag="VNU01_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31992.1"
                     /translation="MRAAARPADARRLWHAEEAVHAVVYFAPETAQAARDAGLKGFWM
                     GYFAGRAAPMGAVGAAAVTAAFFNFHPAMVRRAIPDAWTFAAPDAVLRTRVAAVSRAL
                     DRLLGGQDGVPEAVRLAARAVDGLDCAGRPLAAAWSAWVRDAGPSEPPAALWAACTVL
                     REHRGDGHVAALVDAELDGCQAHVLAAASGATPRESLQPHRGWTDEDWEAAEDALRGR
                     GLLDGAGALTEAGRALRERVEARTDELATAPLAALGTDGAERLVAALRPLAAAVRDGD
                     AIPFPNPMGLPRPEGPS"
     gene            3189..4361
                     /locus_tag="VNU01_04920"
     CDS             3189..4361
                     /locus_tag="VNU01_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWH31993.1"
                     /translation="MIVEPSERARGARSSIIREILRLTQDGAVLSLAGGIPAAEAIPL
                     EELREAAATLPVAAFQYTPTEGYGPLRALLAQRASQELDRPVGEEQVLVTGGSQQALD
                     LLGRVLLDPGDVVAMEHPGYLGAIQALEAWQPSFLPIDVDRDGMRVDLLARALAGGAR
                     PKLVYTVPDFSNPSGATLAAERRRELAALADRYGFLIVEDDPYGHLRFAGARQPAIAT
                     LSDRVVRLQTVSKSLAPGLRVGWVVGPADLTGLLVRAKQAADLQASTTGQALAAALLG
                     RPGWAERQAARVTPLYAERARALSAALRRHAADVLDVPEPEGGMFLWGRVTTPGADTD
                     ALLRAAIAEGVAFVPGGAFAVPGGGSLRDGLRLSYATLDLADLDEAARRLAAALHR"
     gene            4431..4781
                     /locus_tag="VNU01_04925"
     CDS             4431..4781
                     /locus_tag="VNU01_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31994.1"
                     /translation="MNGTIAASGGITLDGGEAARVTELLEMLGELPSTPDFVAGEARA
                     RARALAGAMPAPVHHLGPGARPPAAPVSLDRLSTTGIAGLLELAAGMPSTPPDVADDA
                     LSYADLLWGLLSQG"
     gene            4848..5363
                     /locus_tag="VNU01_04930"
     CDS             4848..5363
                     /locus_tag="VNU01_04930"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH31995.1"
                     /translation="MQQMPLDSAEARVEIAAPVAKVWELVGDPLRCGDWSPENLGGRW
                     LDGATRPALGARFRGTNRRGLAWWYTNCTVVRYEPERAIAWEVRVPWGGPPLSRWTYE
                     LAPSAAGTEVTERWELLNASALTRATWVLVAGSPAARVAMLKASLATSLGKLKSVLER
                     DVVAAGEGGQA"
     gene            complement(5354..6934)
                     /locus_tag="VNU01_04935"
     CDS             complement(5354..6934)
                     /locus_tag="VNU01_04935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH31996.1"
                     /translation="MAADLTRERWTLTAVVLASSVVFLDSTLVGVALPRIAEELGSGP
                     FGVLEAQSYVYNGYLLTLSALLVLAGAASDRYGRRRMFLVGLAGFTATSVACGLAPSM
                     PALIVARLLQGASGAVLVPGSLALIASVFTGARRGWAYGVWAAASAATTILGPLLGGA
                     LVDTVSWRAAFLLNVPLLAVAIWATLRHVPESRDEEAARGFDWLGAALIATGLGGLTY
                     GAIRGQEAQWQDTGAFVALAVGALATALFVPVQARRRNPLVPLGLFRERNFAAINAST
                     LLIYGALYVASYLTVIHLQGTLGYSATAAGVALVPMSLLLAGLSSRFGALAARLGPRR
                     FLTAGPLLMAAGLLWLVRMPADSAAWDLRPASPATWPPPADYVVDLLPMAVVFGLGLA
                     VMVAPLTTALMASIPPRHAGLASAINNAISRVGPQLAGAAVFVAVTAGFYADVAGRAG
                     VDDTAALRAAVAPLNAPAEGAPPGVAAAARGASARAHHQAMAIAAGLLAAGAAVNGLA
                     VRDEELRLDDPDEAVPQA"
     gene            7032..7766
                     /locus_tag="VNU01_04940"
     CDS             7032..7766
                     /locus_tag="VNU01_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH31997.1"
                     /translation="MSSVIQRDQRTIGGVRRRPTGGRWGGVRLALLIGLAGALPAFLG
                     VWALNSAQPTVAERRAELAAYLGELKPLAEETGFLVIHGLRAGVNDIGTGAFSDEILQ
                     QQPVGWRRDLEQLKDKFAAVKAPGGLEDAHQALAATLDGYIEVTHQLEAAAHAPVYRR
                     RMLVRRAADLGQRIDREWNVGAYQIQWALDEVGEDMVIWLPDPTVNPDSDDYVDGDPD
                     EARPNDFMRETGVQRRLRASEAASDA"
     gene            complement(7770..8408)
                     /locus_tag="VNU01_04945"
     CDS             complement(7770..8408)
                     /locus_tag="VNU01_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH31998.1"
                     /translation="MQDAATIRVVIVEDHTLVREGLRSLLTAAPDIEVAGYGRCVAEA
                     LEVIEATSPHVVLLDLRLGEEDGLEVSRSLSSHPAGPEVLVLSAHERPSDLRAALNAG
                     ATGYLLKHTTPRLLVDAVRRVSGGHRVIDQAFVPLLVGLPSQTAELPDLTPREQQVLD
                     LVAEGLANREIGERLGIARSTAQKHLESLFRKFDVHDRSGLVAKAFRSRYLE"
     gene            complement(8548..>8883)
                     /locus_tag="VNU01_04950"
     CDS             complement(8548..>8883)
                     /locus_tag="VNU01_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWH31999.1"
                     /translation="AIDLDIHLPQAGMPRQTFGSALVTSVGMFGIQHAYAPLSPYYRV
                     PYLVLVGEVADKPVVVDGEIVIRPMLNLAATMDHRYLDGFHAARLARSVRAYCADPFA
                     FEPPLPPAG"
BASE COUNT         1160 a         3233 c         3389 g         1101 t
ORIGIN      
        1 gagaccggca tgatcgtgcc gctgggcgac tgggtcctgg agcacgcggc ggccgaggtg
       61 gcggcgtggc aggcccgctt ccgccggccc atcgacctgg ccgtcaacgt gtcggcgcga
      121 cagctcgacc gtgccgggtg gcccgccggg gtcgccgcca tcctggagcg ctccggcctg
      181 ccggccacga acctccacct ggagctcacc gagaccgccc tgttcgagtc cggcaccagc
      241 accgtgggcg ccttccgcgg cctgcgcgcc cagggcgtgc acatcggcat cgacgacttc
      301 ggcaccggct actcgtcgat gagctacctg cgtgacttcc cggtcgactt cctgaagatc
      361 gaccgcgcgt tcgtcagcga gctcgacagc cggtcgcgcg agtcgacggc gatcgtcgag
      421 gcgatcctgg cgctggggcg ggcgctgggc ctgcgcaccg tcgccgaggg cgtggagacc
      481 gccgcgcagg cggagcggct ggcggcgctg gactgcgacc tggcgcaggg ctacctgctg
      541 ggccggccca tgccggcggc ggagttcgag gcggtgctgg ccgcccagcc cacctcgctg
      601 gcgggcatgc gggcgtacgg caccgggtag gcgagtcggt gccatggaca ccgactccgc
      661 tgcgggcgcg acgcccacgc cctacaccgc ccagcacatc aaggacgccc tcgcgcagga
      721 cccgcgcgtg ggcgagatga ccttcgaggt caccctcgcc ggcggccgcg ccttcgtcgc
      781 cgggcaggtg ccgaccgagc agcgccgcga cgccgtcccc gacgtcgtgc gcagcgtcgc
      841 cccggacctg gaggtggtca acgaggtgac cgtcgtcccc tgcgacgagc cggtcggcca
      901 ggaggtgatc aggtgatccg catcgccgcc accggcgacg tgcacttcgg cgaggacagc
      961 ggcggcacgc tgcggccgca cctggagcac ctgggcgaca acgccgacct gttcctgatc
     1021 gcgggcgatc tcaccaactg gggcacggtc gccgaggcgc aggtcctggc cgaggagctg
     1081 cgcgacctgc cggtcccggt cgtgctcgtg ctgggcaacc acgactacca gtccgacgag
     1141 cccgacaaga tccgccaggt gctcaccgac gccggcgtgg tggtcctgga gggcgagagc
     1201 gccaccttcg acgtccgcgg acagaaggtc ggcatcgccg ggaccaaggg gttcggcggc
     1261 ggcttcgcgg gcgcgtgcgg cacggcgttc ggcgagccgg agatgaaggc gttcatcgac
     1321 cacacgcagc ggctcgccga ccggctggag cgctgcctga cggacctgga cgccgaccac
     1381 aaggtggcgc tgatgcactt ctcgccggtg gagcagacgc tgcgtggcga gcgcctggag
     1441 atctacccgt tcctgggcag ctacctgctg gcggaggcct gcgaccgtgg cggcgccgac
     1501 ctgatcgtgc acgggcacgc ccatgccgga acggagaagg gcgtgaccgc cgggggcgtg
     1561 cacgtgcgca acgccagccg cttcgtgatc cgccacgcgt acaaggtcta ttgcctggga
     1621 ggagccggcg acgtggcctg cgacgacagc gcggcggcgt gaggcgcaca tggcgaccca
     1681 ggacgagctg cgcgaaagca tgaagaaggc cgccgcggcg ctgcgtgacg ccgacgtgcc
     1741 gttcatcctg gccggttcca tggcgtgctg ggctcgcggg gggcccgtca gccagcacga
     1801 cgtcgatttc atggtcaagc cggaggacgc cgaccgcgcc gtgaaggccc tggaggaggc
     1861 cggcctgcgc atcgagcggc cgccggaggg atggctgttc aaggcgtggg acggtgacgt
     1921 gctggtcgac ctgatctggt cgcccagcgg catggagatc accgacgagg tgttcgcgcg
     1981 cgcggacgcg ctcaacgtcc acgccgtgac catgcgcgtg atgcgccttg aggacgtgat
     2041 cgtcaccaag ctggcggcgc tgcgggagca cgagctggac tacgagcgca tcctggagat
     2101 cagccgctcg ctgcgcgagc tgatcgtctg ggacgaggtc cgccgcgagg cgccggacaa
     2161 cccgtacgtg cgtgccttct tcaccatgat cgaaggcctc ggcatcgccc cgcccagcga
     2221 ggcgcacacc gccgcgacgc cctgaccggc cgtccactgg ctcgtgcact ggccctgggc
     2281 cggcctggcc gcctcggcga cgatgaggcc atgcgcgccg cggctcgtcc tgccgatgcc
     2341 cgccgcctct ggcatgccga ggaggcggtg cacgccgtcg tgtacttcgc gccggagacc
     2401 gcgcaggcgg ccagggacgc cgggctcaag ggcttctgga tgggctactt cgccggacgc
     2461 gccgcgccga tgggggccgt gggcgccgcc gcggtgacgg ccgccttctt caacttccac
     2521 cccgcgatgg tgcgccgcgc catccccgac gcctggacgt tcgcggcgcc ggatgcggtc
     2581 ctgcggacgc gcgtcgccgc ggtgtctcgg gcactcgacc ggctcctcgg cggccaggac
     2641 ggcgttcccg aggcggtgcg gctggcggcg cgcgccgtcg acgggctgga ctgcgccggg
     2701 cggcccctgg ccgcggcctg gagcgcgtgg gtccgcgacg ccggcccgtc ggagccgcct
     2761 gccgcgttgt gggcggcctg cacggtcctg cgggagcatc gcggcgacgg gcacgtcgcg
     2821 gcgctcgtcg acgccgaact ggacggctgc caggcccatg tgctggcggc ggcctccggc
     2881 gcgacccccc gggagtcgct gcagccccac cgcggctgga cggacgagga ctgggaggcg
     2941 gccgaggacg cgctgcgtgg ccgcggcctg ctggacggcg ccggcgccct gacggaggcg
     3001 ggccgggcac tgcgcgaacg cgtcgaggcg cgcacggacg aactggccac ggcgcccctc
     3061 gcggcgctgg gcaccgacgg ggcagagcgg ctggtcgcgg cgctgcggcc gctggccgcc
     3121 gccgtccgtg acggcgacgc catcccgttc cccaacccca tgggcctgcc acggccggag
     3181 ggcccgtcgt gatcgtggag ccgtccgagc gcgcccgcgg ggcgcgcagc tccatcatcc
     3241 gcgagatcct ccggctgacg caggacggcg ccgtgctgtc cctggcgggc ggcatccccg
     3301 ccgcggaggc gatcccgctg gaggagctgc gcgaggccgc cgcgacgctg ccggtcgcgg
     3361 ctttccagta caccccgacg gaaggctacg gccccctgcg cgcgctgctg gcgcagcggg
     3421 cctcgcagga actggaccgg cccgtcggcg aggagcaggt gctggtcacc ggcggctcgc
     3481 agcaggcgct ggacctgctg ggccgcgtgc tgctggaccc gggcgacgtg gtcgccatgg
     3541 agcatcccgg ctacctgggc gcgatccagg cgctggaggc gtggcagccg tcgttcctgc
     3601 cgatcgacgt ggaccgcgac ggcatgcgcg tggacctgct ggcccgggcg ctggcgggcg
     3661 gcgcccggcc gaagctggtg tacacggtgc cggacttctc caatcccagc ggggccacgc
     3721 tcgcggcgga gcgacggcgg gagctcgccg cgctcgccga ccgctacggc ttcctcatcg
     3781 tcgaggacga cccgtacggc cacctgcgct tcgcgggtgc ccgccagccg gcgatcgcca
     3841 cgttgtcgga ccgcgtggtc cgcctgcaga cggtgtcgaa gtcgttggcg cccggcctgc
     3901 gcgtcggctg ggtggtgggc ccggcggatc tgaccgggct gctggtccgg gcgaagcagg
     3961 cggcggacct ccaggcgtcc accaccggcc aggcgctcgc cgccgccctg ctgggccgcc
     4021 cgggctgggc cgaacggcag gccgcgcgcg tcaccccgct gtacgccgag cgcgcccgcg
     4081 cgctgtccgc tgcgctgcgg cgccacgccg cggacgtcct ggacgtcccc gagccggaag
     4141 gcgggatgtt cctgtggggc agggtgacca cgcccggcgc cgacaccgac gcgctgttgc
     4201 gcgcggccat cgccgagggc gtggcgttcg tccccggcgg ggcgttcgcc gtgcccggcg
     4261 ggggttcgct gcgcgacggc ctgcggctgt cctacgcgac gctggacctg gcggacctgg
     4321 acgaggccgc ccggcgcctg gccgccgccc tccaccgata ggaatgcgtt ttccatccgt
     4381 ctctggcgga gacggtggca gccatccagt gggcgaggtc acgcatgggc atgaacggca
     4441 cgatcgcggc aagtggcggc atcaccctcg acggtgggga ggccgccaga gtcaccgagc
     4501 ttttggagat gctgggtgaa ctgccctcca cgccggactt cgtggccggc gaggcgcgag
     4561 cgcgagcgcg tgcgctcgcg ggggccatgc ccgcgccggt ccaccatctc ggcccgggtg
     4621 cgcggcctcc ggccgccccc gtgtcgctgg accgtctcag caccacgggc atcgccgggc
     4681 tgctggagct ggcggcgggc atgccgtcca cgccaccgga cgtggccgac gacgcgctgt
     4741 cgtacgccga cctgctgtgg ggcctgctga gccagggctg accggcgtat cgttcgtcgc
     4801 cggacggccg gacggcgggt ccagcctggt gggaagggac ggacggcatg cagcagatgc
     4861 ccctggacag cgcggaagcg cgcgtggaga tcgccgcgcc ggtggcgaag gtgtgggagc
     4921 tggtcgggga tcccctgcgc tgcggcgact ggagcccgga gaacctgggc gggcggtggc
     4981 tggacggcgc cacccgtccg gccctgggcg ctcgcttccg cggcaccaac cggcgcggcc
     5041 tggcgtggtg gtacaccaac tgcaccgtgg tgcgctacga gcctgagcgc gccatcgcct
     5101 gggaggtccg ggtgccctgg ggcggcccgc cgctgtcgcg ctggacctac gagctggcgc
     5161 cctcggcggc cggcacggag gtcaccgagc ggtgggagct gctcaacgcc agcgcgttga
     5221 cgcgcgcgac ctgggtgctg gtcgcgggca gccccgcggc gcgcgtggcg atgctgaagg
     5281 cctcgctggc cacgtcgctg ggcaagctca agagcgtgct ggagcgcgac gtcgtggcgg
     5341 ccggcgaggg cggtcaggcc tgagggacgg cctcgtcggg atcgtccagc cgcagctcct
     5401 cgtcgcgcac cgcgagtccg ttcaccgctg ccccggcggc cagcaggccc gccgcgatcg
     5461 ccatcgcctg gtggtgggcg cgcgccgagg cgccccgcgc ggccgcggcg acgcccgggg
     5521 gggcgccctc cgccggcgcg ttgagcggcg ccaccgccgc gcgcagcgcc gcggtgtcgt
     5581 cgacgccggc gcgcccggcg acgtccgcgt agaacccggc ggtgaccgcg acgaacacgg
     5641 cggccccggc cagttgcggg ccgacgcggg agatggcgtt gttgatggcg ctggccagcc
     5701 ccgcgtggcg cggcgggatc gacgccatga gggcggtggt cagcggcgcc accatcacgg
     5761 ccaggcccag gccgaagacc acggccatgg gcagcaggtc gacgacgtag tcggccggcg
     5821 gcggccacgt cgccgggctg gcgggacgca ggtcccaggc ggcgctgtcc gccggcatgc
     5881 gcaccagcca cagcagcccg gcggccatca gcagcggtcc ggcggtgagg aagcgccgcg
     5941 gtcccagccg tgcggccagc gcgccgaacc gcgacgacag cccggcgagc agcagtgaca
     6001 tcggcacgag cgccaccccc gccgccgtgg cgctgtagcc cagcgtgccc tgcaggtgga
     6061 tcaccgtgag gtagctggcg acgtacagcg cgccgtagat gagcagcgtg gaggcgttga
     6121 tcgcggcgaa gttgcgctcg cggaacagcc ccagcggcac cagcgggttg cgccgccgcg
     6181 cctgcaccgg cacgaacagc gcggtcgcga gcgccccgac cgccagggcc acgaacgcgc
     6241 cggtgtcctg ccactgcgcc tcctgcccgc ggatcgcgcc gtaggtcagc ccgcccagcc
     6301 ccgtggcgat cagcgcggcg cccagccagt cgaagccgcg ggcggcctcc tcgtcgcggc
     6361 tctccggcac gtggcgcagc gtggcccaga tcgccacggc caacagcggc acgttcagca
     6421 ggaacgccgc ccgccagctc accgtgtcca ccagcgcgcc gcccagcagg gggcccagga
     6481 tggtcgtggc ggccgaggcc gcggcccaga cgccgtaggc ccacccgcgc cgcgcacccg
     6541 tgaacaccga ggcgatcagc gccagcgacc ccggcaccag cacggccccg ctggcgccct
     6601 ggagcaggcg cgccacgatc agggccggca tggatggcgc caggccgcac gccacggacg
     6661 tggccgtgaa cccggccagg ccgaccagga acatgcgccg gcgcccgtag cggtcgctcg
     6721 ccgcgccggc cagcaccagc agcgcggaca gcgtgagcag gtagccgttg tagacgtagg
     6781 actgcgcctc caggacgccg aacggcccgc tgcccagttc ctcggcgatc cggggcaggg
     6841 cgacgccgac cagcgtcgag tcgaggaaca cgacgctgga ggccagcacc acggcggtca
     6901 gggtccagcg ctcgcgggtc aggtcagcgg ccatgcggca tcttcgcact ccccgcgcac
     6961 ggattcgccg tggaaccgcg ccgccgggcc atgcgttgct accctcgacg gtcgagacgg
     7021 gaaggcgggc gatgtcctcg gtgatccaac gggaccagcg caccatcggc ggcgttcggc
     7081 ggcggcccac ggggggccgc tggggcggcg tgcgcctggc gctgctgatc gggctggccg
     7141 gcgccctgcc ggcgttcctg ggcgtgtggg cgctcaacag cgcccagccg accgtggcgg
     7201 agcgccgcgc ggagctcgcc gcctacctgg gcgagctcaa gccgctggcg gaggagaccg
     7261 ggttcctggt catccacggg ttgcgcgccg gcgtcaacga catcggcacc ggggcgttca
     7321 gcgacgagat cctgcaacag cagccggtgg gctggcgccg cgacctcgag cagctcaagg
     7381 acaagttcgc ggcggtgaag gcgccgggcg gcctcgagga cgcgcaccag gcgctggcgg
     7441 cgacgctcga cggctacatc gaggtgacgc accagctcga ggcggcggcg cacgcgccgg
     7501 tctaccggcg gcgcatgctg gtgcgccggg cggccgatct cggccagcgc atcgaccgcg
     7561 agtggaacgt gggcgcgtac cagatccagt gggccctcga cgaggtcggc gaggacatgg
     7621 tgatctggct gcccgacccc acggtgaacc cggactccga cgactacgtc gacggcgacc
     7681 cggacgaggc gcgtccgaac gacttcatgc gcgagaccgg cgtgcagcgc cgcctgcggg
     7741 cctccgaggc ggcgtccgac gcctgaggct cattccaggt agcggctgcg gaacgccttg
     7801 gccaccaggc cgctgcggtc gtgcacgtcg aacttgcgga acaagctctc caggtgcttc
     7861 tgggcggtgg accgggcgat gcccaggcgt tcgccgatct cccggttggc caggccctcg
     7921 gcgaccaggt ccagcacctg ctgctcccgg ggtgtgaggt ccggcagctc ggcggtctgc
     7981 gatggcaggc ccaccagcaa gggcacgaac gcctggtcga tcacccggtg cccgccgctg
     8041 acgcggcgca ccgcgtccac caggagccgc ggcgtggtgt gcttgagcag gtagccggtg
     8101 gcgcccgcgt tcagcgcggc ccgcaggtcg ctgggccgct cgtgggccga cagcaccagc
     8161 acctccgggc cggccgggtg gctcgacagc gagcgggaca cctccaggcc gtcctcctcg
     8221 cccaggcgca gatccagcag caccacatgg ggcgaggtcg cctcgatcac ctccagcgcc
     8281 tccgcgacgc agcggccgta gcccgccacc tcgatgtccg gcgccgcggt cagcagcgac
     8341 cgcagcccct cccgtaccag tgtgtggtcc tccacgatga ccacgcggat cgttgcggcg
     8401 tcctgcaccc tcggcctccc cgttgcgtcc tgtcggggtc gggtgtcgtc gcgcatctgt
     8461 cgaggccgaa gggccggatc ggggcatgcc cagggtccaa cgacgtaggc atgtacgtag
     8521 ttcgcgcggg ccgatcgccc acccgggtca gcccgccggg ggcagcggcg gctcgaaggc
     8581 gaaggggtcg gcgcagtagg cgcggacgct gcgggccagt ctggcggcgt ggaagccatc
     8641 cagatagcgg tggtccatcg tggccgccag gttgagcatc gggcggatca cgatctcgcc
     8701 gtcgaccacc accggcttgt ccgccacctc gccgaccagg accaggtacg gcacgcggta
     8761 gtacggcgac aggggcgcgt acgcgtgctg gatgccgaac atgcccacgg aggtgaccag
     8821 ggcgctgccg aaggtctggc gcggcatgcc ggcttgcggc aggtggatgt ccaggtcgat
     8881 cgc
//
LOCUS       DATMKC010000166         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_75811, whole genome shotgun sequence.
ACCESSION   DATMKC010000166 DATMKC010000000
VERSION     DATMKC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75811"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VNU01_04955"
     CDS             complement(<1..977)
                     /locus_tag="VNU01_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH32000.1"
                     /translation="MCSSAGFEIDAALATADLHGLLDVAERALRRAKTLPLGGAVSDV
                     ELTQAVQCLEAVSRVAESHKCAVVGEIEQRHAARGQSAASTEDLLTRVLHLTPGEARA
                     RTELAAGLKQLPDTAAAFADGRMGVGQAAVAVRKAAEVAGRDDAEVLLATIDRTASTV
                     GQTVNRTRLAREIDTAVAKTGVDVLAERERRAFQDRKLQWSVRDGMDVLTAHLDPIGA
                     ATVRGMLDTLSDKTSERDSRTFVQRQADALVDLATLAHTARNIAKTTMPPSKVLLITT
                     PDALHGVPGAEPATIDGHGPVSPELARQLCCDSAVTPVVTGRNGEVLDVG"
     gene            complement(1053..2312)
                     /locus_tag="VNU01_04960"
     CDS             complement(1053..2312)
                     /locus_tag="VNU01_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWH32001.1"
                     /translation="MVGTEWARAGAHADVVVVGGGPAGGAAATLLARAGLSVVILERS
                     LRYRDRVLGEYVLPWGVRHAMDTGLYGTFVAAGANVLTTLIHYDEGWTPARAEAEAVP
                     LDRMVPGVPGALGVSHPGLCAALSDAAVAAGARVLRGVSDVDIRPGEHPWIAFTQHGR
                     RHELRPRLVVGADGRESAVRRAAGMTLTQTPVLAYGTGLLIDGAKEWPDRDEAIANED
                     GKLCFVFPQGSGLVRLYQLVMPDRRGRFRGPGRTERFLEDFRLRSVPGADTLADAEPA
                     GPCATWPMHDGWVREPVAPGLVLIGDAAGYTNPLIGQGLSCALSDARAVAETVLEGGW
                     EAAAFAGYAARRRERGKRLLFTVEMFARHRCITGPGSAEHRVRAHERMKADPLLGQWF
                     TYGYAGPHLAPAEAYADEVRERLWFTD"
BASE COUNT          356 a          987 c          896 g          337 t
ORIGIN      
        1 cccacgtcca ggacctcgcc gttgcggccg gtcaccaccg gcgtcaccgc cgagtcgcag
       61 cacagctgcc gcgcgagctc gggtgagacg ggtccgtgac cgtcgatcgt cgccggctcc
      121 gcacctggga cgccatgcaa cgcgtccggg gtggtgatca gcagcacctt ggacggcggc
      181 atcgtggtct tggcgatgtt gcgggcggtg tgcgccagcg tcgccaggtc caccagcgcg
      241 tcagcctggc gctgcacgaa cgtccggctg tcacgctccg acgtcttgtc cgacagggtg
      301 tccagcatcc cccgcaccgt ggccgcccca atggggtcca ggtgtgcggt gagcacgtcc
      361 atgccgtcac gcacggacca ttggagcttg cggtcctgaa acgcgcggcg ttcccgctcc
      421 gccaacacat ccacaccggt tttcgcgacg gcggtgtcga tctcacgggc caggcgggtg
      481 cggttcacgg tctggccgac cgtcgacgcg gtccggtcaa tcgtggcgag cagcacctcg
      541 gcgtcgtccc ggccggcgac ctcggcggct ttgcgcaccg ccacggcggc ctggcccacg
      601 cccatacggc cgtcggcgaa cgccgccgcc gtgtccggca actgcttcag ccccgccgcc
      661 agctccgtgc gcgccctcgc ctcacccggc gtcaggtgca gcacgcgcgt caacaggtcc
      721 tcggtcgatg cggcggactg gccgcgcgcg gcgtgccgct gctcgatctc gcccaccacc
      781 gcgcacttgt gcgactccgc gaccctgctc accgcttcca ggcactgcac cgcctgcgtg
      841 agctcgacat cggagaccgc accgcccaac ggcagcgtct tcgcgcgacg caacgcgcgc
      901 tcggccacgt ccagcagacc gtgaaggtcc gcggtggcca gcgccgcgtc gatctcgaac
      961 cccgcactcg aacacatgtt cgacacatta tcacgagggt gcgactcgtc aacccacgaa
     1021 attgaatctg tgggcaaccc gtgcaggagc ggtcagtcgg tgaaccacag gcgctcgcgg
     1081 acctcgtcgg cgtaggcctc cgccggggcg aggtgagggc cggcatagcc gtaggtgaac
     1141 cactgcccca gcaacgggtc ggccttcatg cgctcgtgcg cgcgcacgcg gtgctcggcc
     1201 gaccccgggc cggtgatgca gcgatgccgg gcgaacatct ccacggtgaa cagcaggcgc
     1261 ttgccgcgct cgcgccggcg cgcggcgtag ccggcgaagg cggcggcctc ccagccaccc
     1321 tccagcacgg tctcggcgac ggcccgcgcg tcggacagcg cgcaggacag cccctggccg
     1381 atcagggggt tggtgtaccc ggccgcgtca ccgatcagga cgaggccggg cgcgacgggc
     1441 tcgcggaccc agccatcgtg catgggccag gtggcgcacg gcccggccgg ctcggcgtcg
     1501 gcgagcgtgt ccgcgcccgg gacgctgcgc agccggaagt cctccaggaa gcgctccgtg
     1561 cggccgggcc cgcggaagcg gccgcgccgg tccggcatga cgagctggta caggcgcacc
     1621 aggccggagc cctgcgggaa cacgaagcag agcttgccgt cctcgttggc gatggcctcg
     1681 tccctgtcgg gccactcctt cgccccgtcg atgagcagcc cggtgccgta ggccagcacc
     1741 ggcgtctggg tcagggtcat gcccgcggcc cgccggaccg ccgactcgcg cccgtccgcg
     1801 ccgaccacca ggcggggccg cagctcgtgc cgtcgtccgt gctgggtgaa cgcgatccac
     1861 ggatgctccc cggggcggat gtcgacgtcg ctcacaccgc gcagcacgcg ggcgccggcg
     1921 gcgaccgcgg cgtcgctcag cgccgcgcac agccccgggt gcgacacgcc cagcgcgccg
     1981 ggcacgcccg gcaccatgcg gtcgagcggg acggcctccg cctcggcccg cgcgggcgtc
     2041 cagccctcgt cgtagtggat cagggtcgtg aggacgttcg cgccggcggc gacgaaggtg
     2101 ccgtagaggc cggtgtccat cgcgtggcgc acgccccacg gcagcacgta ctcgcccaag
     2161 acgcgatcgc gatagcgcag ggagcgctcc aggatcacca ccgacaggcc tgcccgggcc
     2221 agcagtgtgg ccgcggctcc gccggccggc ccgccgccca ccacgacgac gtccgcgtgc
     2281 gcgccggcgc gcgcccattc ggtccccacc atctcgtact ccccccttga ccgctctccc
     2341 cccggagacg atcaaggaag ctactctgcg tgacgggggg gcgtcaaagc gacacctttt
     2401 ccgtcggcac gcgcacgagg cgcacaagcc gaagcccggt tcttccagaa atgcgtcgaa
     2461 tgttcgatgc gtggaggtcc cggaccgcgg cttcgccgct ggtggcccgt gcggtcagcg
     2521 ccggcggcgc agcgccaggc cacccagggc cagcgccatg ccggccaggg ccgcgg
//
LOCUS       DATMKC010000167         3712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_312621, whole genome shotgun sequence.
ACCESSION   DATMKC010000167 DATMKC010000000
VERSION     DATMKC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312621"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="VNU01_04965"
     CDS             complement(<1..2095)
                     /locus_tag="VNU01_04965"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH32002.1"
                     /translation="MSAGRDGRAVMWSAGRVEQANGESGQGAAPDRRRILVVDDDEMI
                     RELLHEVLTQAGYRVRTADSGDDALAKLHAHVPGLVLSDINMPGMDGFAFLAALRANA
                     ATRAMPLIFLTARDGIQDLVEGFALGADDYVTKPVAIPELLARVRAKLARPPVPLDDL
                     GADPRSGLLSTERLREELRRETLRTRRGGTPGVFAQLSLSELDSIRLRLGTEAERAVL
                     EQLAGLLTGLLRPLDLSGLLGDQRIGVLLPETGPEDAARRLGLALRAISAHAFVAGGS
                     TLRLTPSAGYAPLGGRDADGGPDDAEPRAGVALDYAEAHLDLLPLRWEPRMEAVVEAL
                     RQQRARDRLRLRRDALLRAVRTPLQIAATAVVGLVLPYLGYLWLWRAGHDITGVVYLV
                     VVGALLVTGILIWMEGIAALRRPAPPDEPGAPEPPASAVIAAYLPNEAATIVETIEAF
                     LRIDYPDLEVVLAYNTPEPMAVEARLRGLAKREPRFRPFRVDASTSKAQNVNAALSTL
                     RGEFVGVFDADHHPEPDSFRRAWRWLSNGYDVVQGHCVVRNGDASRLARLVAVEFEAI
                     YAVSHPGRARLHGFGIFGGSNGYWRRDLLHEIRMRGSMLTEDIDSSLRVVEAGGRIAS
                     DPGLISRELGTTTFRQLWNQRMRWAQGWFQVSIRHLWRALRSPGLSVRQKLGALHLLG
                     WREVYPWLSLQMFPII"
     gene            complement(2092..3039)
                     /locus_tag="VNU01_04970"
     CDS             complement(2092..3039)
                     /locus_tag="VNU01_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWH32003.1"
                     /translation="MEDAIVVGGGQARRAAASWLARYRRKVVVIDSGEHRNRWTRAAH
                     GYLGLDPISPEELLERGRRELLAYPTASWRAGKATAARRLDEGFAVEVDGEPVLAHRL
                     VLATGVRDRFPDVEGFLDHYGTSVFHCPACDGYEARDRDVVALGWAPHVAGFALELLD
                     WARSVTVVTGHTRFQGDERHRDALGRNGIRLIEDRAAALTGAPGRLEGVRLRGGETVP
                     AQLLFFSIGHEPASPLAERLGCRMVEGGECVWVDENGQTSVPGVFAAGDMTPGTQLVQ
                     IAAAKGVTAGVACAMSLRGEMTFTESPVPAPDVAAELGE"
     gene            3104..>3712
                     /gene="selD"
                     /locus_tag="VNU01_04975"
     CDS             3104..>3712
                     /gene="selD"
                     /locus_tag="VNU01_04975"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWH32004.1"
                     /translation="MSTRRLTEFSHGAGCGCKLGPGELAAVLAMLPPYAHEDLLLGAD
                     SGDDAAVWRLDDTRALIATVDFFTPIVDDARTWGRIAAANAASDVYAMGGRPLFALNL
                     VSWPREALPLELLGEVLAGAADAAADGGWMAVGGHSIDDPEPKFGQAVIGEAERARLL
                     THRGLRAGDALVLTKPLGVGVITTAVKRGAAPPEAVDAAITSM"
BASE COUNT          489 a         1416 c         1353 g          454 t
ORIGIN      
        1 cgatgatcgg gaacatctgc agcgacagcc acgggtacac ctcgcgccag ccgagcaggt
       61 gcagcgcccc gagcttctgg cgcaccgaca gtcctgggga gcgcagcgcc cgccacaggt
      121 ggcggatgga cacctggaac cacccctgcg cccagcgcat gcgctggttc cagagctggc
      181 ggaacgtggt ggtgcccagc tcgcgcgaga tcaggccggg gtccgacgcg atgcgcccgc
      241 ccgcctcgac gacccgcagc gacgagtcga tgtcctcggt cagcatggag ccccgcatgc
      301 ggatctcgtg cagcaggtcg cggcgccagt agccgttgga cccgccgaag atgccgaacc
      361 cgtgcagccg cgcgcggccg gggtggctca ccgcgtagat cgcctcgaac tccacggcca
      421 ccagccgcgc cagccgtgac gcgtcgccgt tgcgcaccac gcagtgccct tgcaccacgt
      481 cgtagccgtt ggacagccag cgccaggcgc gccggaacga atcgggctcc gggtgatggt
      541 cggcgtcgaa cacgccgacg aactcgccgc gcagggtcga cagcgccgcg ttgacgttct
      601 gggccttgga cgtgctcgcg tccacgcgga acggccgaaa gcgcggctcg cgcttcgcca
      661 gcccccgcag gcgcgcctcg accgccatcg gctcgggcgt gttgtaggcg agcacgactt
      721 cgaggtccgg gtagtcgatg cgcaggaacg cctcgatggt ctccacgatc gtcgccgcct
      781 cgttcggcag gtacgcggcg atcaccgcgc tggcgggcgg ttcgggcgcg ccgggctcgt
      841 ccggcggcgc gggccggcgc agggcggcga tgccctccat ccagatcagg atgccggtga
      901 cgagcagcgc accgacgacc acgaggtaga ccacaccggt gatgtcgtgg ccggcgcgcc
      961 agagccagag gtagccgagg tagggcagga ccagtccgac cacggcggtc gccgcgatct
     1021 gcaacggggt gcgcacggcg cgcagcagcg cgtcccgccg cagccgcagt cggtcgcgcg
     1081 cacgctgctg gcggagcgcc tcgacgacgg cctccatgcg cggctcccag cgcagcggca
     1141 gcaggtccag gtgcgcctcc gcgtagtcca aggccacccc ggcgcgcggc tcggcgtcgt
     1201 ccgggccgcc gtccgcgtcg cgcccgccga ggggcgcgta gccggcgctg ggcgtgaggc
     1261 gcagcgtgga gccgccggcg acgaacgcgt gcgcggagat cgcccgcagc gccaggccca
     1321 ggcggcgcgc ggcgtcctcc ggaccggtct ccggcaggag cacgccgatg cgctggtcgc
     1381 cgaggaggcc gctgagatcc agcgggcgca gcaggcccgt gagcagcccg gcgagctgct
     1441 ccaggacggc gcgctcggcc tccgtgccca ggcgcagcct gatcgagtcg agctcggaca
     1501 gcgacagctg cgcgaacacc ccgggggtgc cgccgcggcg cgtgcgcagg gtctcgcggc
     1561 gaagctcctc gcgcagccgc tcggtggaca gcaggccgct gcgaggatcc gcccccaggt
     1621 cgtccagggg caccggcggt ctggcgagct tggcccgcac ccgtgccagc agctccggga
     1681 tcgccaccgg cttggtgacg tagtcgtccg cgcccagcgc gaagccctcg accaggtcct
     1741 ggatgccgtc acgggcggtc aggaagatca gtggcatcgc gcgcgtcgcg gcgttcgcgc
     1801 gcagcgccgc caggaacgcg aacccgtcca tgccgggcat gttgatgtcg gacaggacca
     1861 ggccgggcac gtgcgcgtgg agcttggcca gcgcgtcgtc gcccgagtcc gcggtccgca
     1921 cgcgatagcc ggcctgtgtc agcacctcgt gcagcagctc gcggatcatc tcgtcgtcgt
     1981 cgacgacgag aatgcgccgc ctgtccggcg ccgcgccctg gcctgattcc ccgttggcct
     2041 gctccacgcg cccagcagac cacatcacgg ctcgcccgtc ccgcccggcg ctcattcgcc
     2101 cagctcggcc gccacgtccg gggcgggcac gggtgactcg gtgaacgtca tctcgccgcg
     2161 caggctcatc gcgcaggcga cccccgcggt cacgcccttg gccgccgcga tctgtacgag
     2221 ctgtgtgccc ggggtcatgt cgccggccgc gaacacgccc ggcacgctgg tctggccgtt
     2281 ctcgtccacc cagacgcact cgccgccctc caccatgcgg cagccgagcc gctcggccag
     2341 cgggctggcg ggctcgtggc cgatcgagaa gaacagcagc tgtgcgggca ccgtctcgcc
     2401 gcctcgcagg cgcacgccct ccagcctgcc cggtgcgccg gtcagcgcgg ccgcccggtc
     2461 ctcgatcagc cggatcccgt tgcgccccag cgcgtcccgg tgccgctcgt cgccctggaa
     2521 ccgggtgtgg ccggtcacca cggtgaccga ccgcgcccag tccaacagct ccagggcgaa
     2581 gccggcgacg tgcggggccc agcccagcgc cacgacgtcg cgatcgcgcg cctcgtagcc
     2641 gtcgcaggcc gggcagtgga acaccgaggt gccgtagtgg tcgaggaacc cttcgacgtc
     2701 ggggaagcgg tcgcgcaccc ccgtcgccag gacgaggcgg tgggccagca ccggctcgcc
     2761 gtccacctcc acggcgaagc cctcgtcgag cctgcgcgcc gccgtggctt tgccggcgcg
     2821 ccacgatgcg gtcgggtagg ccagcagctc gcggcggccc cgctccagca gctcctcggg
     2881 actgatcggg tccaggccca ggtagccgtg cgccgcgcgc gtccagcggt tgcggtgctc
     2941 gccgctgtcg atcaccacca ccttgcggcg gtagcgcgcc agccagctcg ccgcggcccg
     3001 gcgggcctgg ccgccgccca ccacgatcgc gtcctccatg gcggcatgga tgccctggcg
     3061 cgcccgcgct cacgcgccga cccccggcgg ttaccatcgc cgcatgtcca ccaggcgcct
     3121 caccgagttc agccacgggg ccggctgcgg ctgcaagctc ggcccgggcg agctcgccgc
     3181 cgtgctggcc atgctgccgc cgtacgcgca cgaggacctg ctgctgggcg ccgacagcgg
     3241 cgacgacgcg gccgtgtggc gcctggacga cacgcgcgcg ctgatcgcca cggtggactt
     3301 cttcacgccg atcgtggacg acgcgcgcac atgggggcgc atcgccgccg cgaacgccgc
     3361 cagcgacgtc tacgccatgg gcggccggcc gctgttcgcc ctgaacctgg tgagctggcc
     3421 gcgcgaggcg ctgccgctgg agctgctcgg cgaggtgctg gcgggcgcgg ccgacgccgc
     3481 cgccgacggc ggctggatgg ccgtgggcgg ccactccatc gacgaccccg agccgaagtt
     3541 cggccaggcg gtgatcggcg aggcggagcg cgcccggctg ctcacccacc gcgggctgcg
     3601 cgccggcgac gcgctggtgc tcaccaagcc gctgggcgtc ggggtgatca ccacggcggt
     3661 caagcgcggc gcggcgccgc cggaggcggt cgacgcggcg atcacgtcga tg
//
LOCUS       DATMKC010000168         3936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_181629, whole genome shotgun sequence.
ACCESSION   DATMKC010000168 DATMKC010000000
VERSION     DATMKC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181629"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1341)
                     /locus_tag="VNU01_04980"
     CDS             complement(136..1341)
                     /locus_tag="VNU01_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWH32005.1"
                     /translation="MRGHFVSLTWEHDPTLYAPARYRRACKYDAFVPLPLAGADLNCD
                     AQLTGLLSDAERALQHLGSGGESLAPLARLLLRTESIASSKVEGLHVDVRELARGEAR
                     ADTTGKVSPTTLEVLANIDAMELAIHEATATRRFTPEQIVSIHRLLMAGAPNAHVAGR
                     VRTGQNWIGGNDYNPCGADFVPPPPEHVAPLLDDLCEAINDESLAPLAQAALVHAQFE
                     TIHPFDDGNGRTGRALIHVVLRRRGLTPSFVPPISVEFARARDRYISGLTAYRRGDVA
                     AWLEHFVGAAARAAHLADAYLGAVASLREEWRTRLAGGPSPRSDAAAWAVIDVLPAHP
                     VITAAVACAATGRAKAAVHMALDQLAEADVLVPLSGGRRNRSWEAAGLLDLIAELEAG
                     QNPGRAVRG"
     gene            complement(1415..2251)
                     /gene="mutM"
                     /locus_tag="VNU01_04985"
     CDS             complement(1415..2251)
                     /gene="mutM"
                     /locus_tag="VNU01_04985"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWH32006.1"
                     /translation="MTQLPEVEVLRKDLEKELVGKRFKDATVKNAQLVSRHRNRPDFT
                     KALVGRKIDAIQRRGTWLVLELDEGMAMAMHLGDQGSLTRETASEPPGKHTGAVLTFT
                     TGGALHVHDPSKEAELYAATREEVDEMPELQLKGIDPLAQTFTWHALAQELKTRDKPL
                     KALLVDESFIVGLGDLYSDEILWTAGLSGTRKSATLSSQEVRRLYRAVLEVLYEAVKQ
                     GGTAEGGDADQTDLFGEEGGFGEYLRVYDREGLPCGRCRQPIVLGKIGKLESFYCANC
                     QT"
     gene            complement(2291..3061)
                     /gene="rnc"
                     /locus_tag="VNU01_04990"
     CDS             complement(2291..3061)
                     /gene="rnc"
                     /locus_tag="VNU01_04990"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWH32007.1"
                     /translation="MSTRLETQLGVRFSDPELLRLALTHRSHAFEAGGLPTNERLEFL
                     GDAVLGLVVTDWIYHRFTEASEGRLAKVRAAAVNTHSLADVARGLGLGEEVLLGKGEE
                     QSGGRDKDSILADTLEAVLGAVYLDQGHEPVAELIRRLFSELLEDISRRRESLDYKTS
                     LQELTAGELGLLPLYELSEEGPDHEKQFTAVVSVAGESLGQGVGRSKKEAEQAAAQEA
                     YAVLRQRFAVVSDLSATPAADGAPAPQADVADPAPESA"
     gene            complement(3058..3336)
                     /locus_tag="VNU01_04995"
     CDS             complement(3058..3336)
                     /locus_tag="VNU01_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWH32008.1"
                     /translation="MSDVVTREEILALLMDRLSSVLGLGDEELDEASHFDEDLHADSL
                     DLVEVIESVERALAERGVYITMSDEELVALRTVGEAADKLAELAEGEG"
     gene            complement(3333..>3936)
                     /locus_tag="VNU01_05000"
     CDS             complement(3333..>3936)
                     /locus_tag="VNU01_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32009.1"
                     /translation="AGGQRGAGLFALGRLPGVRRPAVAALLPFGETGTVLVDAGASAD
                     VTAELLVGHARMGVAYAQVLGVAAPRVGLLNIGAEAGKGNALAREAHELLAAALGPAF
                     GGNVEPDAVVAGAVDVVVADGFTGNVFLKTVEALGGDDEHGGAAVLLGVTGNVLVAHG
                     AAGEAEIAAALRTAARVAGQDLAGRVADAMSGAVDEGGGT"
BASE COUNT          540 a         1492 c         1326 g          578 t
ORIGIN      
        1 gcctgcgagg tgatcgagct gccgctggac cacgacgccg ccgtgtccgg cgcgcactcc
       61 ttcgtccccg ccctcgtgaa ggccgtcacc accgtcctcg acgcatcctc gccaagcgca
      121 acgggccgct agccctcagc cgcgtaccgc ccgtccaggg ttctggccgg cctcgagctc
      181 ggcgatgagg tcgagcagcc cggctgcctc ccacgagcgg ttccgccgtc ctccgctgag
      241 cggaaccagg acgtccgcct ccgcgagctg gtcgagcgcc atgtgcacgg ccgccttcgc
      301 gcgtcccgtc gccgcgcacg cgaccgccgc cgtgatgacc ggatgcgcgg gcagcacgtc
      361 gatcaccgcc caggccgccg cgtccgatcg tggactcggc cccccggcga gccgcgtccg
      421 ccactcctcg cgcaacgagg cgaccgcgcc gaggtaggcg tcggccagat gcgccgcacg
      481 agcagcggcg cccacgaagt gctcgagcca cgccgcgaca tcgcctcggc ggtacgcggt
      541 caggcccgag atgtagcggt cccgcgcacg ggcgaactcg acgctgatcg gcggcacgaa
      601 cgacggtgtg agcccacggc ggcgcaggac gacgtggatg agggcgcgcc cggttctccc
      661 gttcccgtcg tcgaacgggt ggatcgtctc gaactgcgcg tggacgagcg cggcttgcgc
      721 cagcggcgcg agcgattcgt cgttgatcgc ctcgcacaga tcgtccagga gtggcgcgac
      781 atgctcgggg ggcggcggga cgaagtcggc gccgcacggg ttgtagtcgt tgccaccgat
      841 ccagttctgg ccggtgcgca cccggccggc gacgtgagcg ttgggcgcgc cggccatgag
      901 cagccgatgg atcgagacga tctgttcagg cgtgaagcgc cgcgtcgccg tcgcctcgtg
      961 gatggccagc tccatcgcgt cgatgttggc gagcacttcc aacgtggtcg ggctgacctt
     1021 tcccgtcgtg tccgcgcgcg cctcaccacg cgcgagctcg cgcacgtcga catgaagtcc
     1081 ctcgaccttc gacgacgcga tcgactcggt gcgaaggagc agccgcgcaa gcggcgcgag
     1141 ggactccccg cctgagccca agtgctggag agcacgctcc gcgtccgaca gcaggccagt
     1201 gagctgcgca tcgcaattga ggtccgctcc ggcgagcggg agcgggacga aggcgtcgta
     1261 cttgcaggcg cggcgatacc gagccggcgc gtacagcgtc ggatcgtgct cccatgtcag
     1321 cgacacgaag tgaccgcgca tcctgaacga cctcccagcc ttagttcagt ttaacagcta
     1381 agctgaacta agcgtacggt gtcgttcagg aaggctacgt ctggcagttg gcgcagtaga
     1441 acgactccag tttgccgatc ttgccgagca cgatcggctg gcggcagcgg ccgcacggca
     1501 gcccctcccg gtcgtagacc cgcaggtact cgccgaagcc gccctcctcg ccgaacaggt
     1561 cggtctggtc ggcgtcgccg ccctcggccg tgccgccctg cttgaccgcc tcgtagagca
     1621 cctccaggac ggcccggtac aggcggcgca cctcctggct ggacagcgtg gccgacttgc
     1681 gggtgccgga cagccccgcc gtccacagga tctcgtcgga gtacaggtcg ccgaggccca
     1741 cgatgaagct ctcgtccacc agcagcgcct tcagcggctt gtcgcgggtc ttgagctcct
     1801 gcgccagcgc gtgccaggtg aacgtctgcg ccagggggtc gatgcccttg agctgcagct
     1861 cgggcatctc gtcgacctcc tcacgggtgg ccgcgtagag ctcggcttcc ttgctcgggt
     1921 cgtgcacgtg cagcgccccg ccggtggtga acgtcagcac ggcaccggtg tgcttgccgg
     1981 gcggctcgga ggcggtctcg cgcgtcaggg agccctggtc gcccaggtgc atcgccatcg
     2041 ccatgccctc gtccagttcc aggaccagcc acgtgccccg gcgctggatc gcgtcgatct
     2101 tgcggccgac cagcgccttg gtgaaatccg gccggttgcg gtgcctggag acgagctggg
     2161 cgttcttgac ggtcgcgtcc ttgaagcgct tgcccacgag ctccttctcg agatccttgc
     2221 gcaggacctc gacctcgggc agttgcgtca ccggtccctc cgacctctcc tcagctcgac
     2281 acagctcgac tcaggcggac tctggcgccg gatccgccac gtccgcctgg ggcgccggcg
     2341 cgccgtcggc cgcgggcgtc gcgctgaggt cggacactac cgcgaaacgc tgccggagca
     2401 cggcatatgc ctcctgggcg gcggcctgct cggcctcctt cttgctgcgg ccgacacctt
     2461 ggccgagcga ctccccggcc accgagacca cggcggtgaa ctgcttctcg tggtcggggc
     2521 cctcctcgga cagctcgtac agcggcagca gccccagctc gccggccgtg agctcctgca
     2581 gggaggtctt gtagtccagc gactcgcggc ggcgggagat gtcctccaag agctcgctga
     2641 acagccgccg gatcagctcc gccacgggtt catgcccctg gtccaggtac accgcgccca
     2701 gcacggcctc cagggtgtcg gccaggatgg agtccttgtc ccggccgccg gactgctcct
     2761 cgcccttgcc cagcagcacc tcctcgccca ggcccaggcc ccgggccacg tcggcgaggc
     2821 tgtgggtgtt caccgccgcc gcccggacct tcgccagacg gccctcgctg gcctcggtga
     2881 agcggtggta gatccagtcg gtcaccacca ggcccaggac cgcgtcgccc aggaactcca
     2941 ggcgctcgtt cgtgggcagc ccgcccgcct cgaaggcgtg gctgcgatgc gtgagggcca
     3001 ggcgaagcag ctccgggtcc gagaaccgga ccccgagctg cgtctccagg cgtgtgctca
     3061 tccttcgccc tctgccagtt cggcgagctt gtcggccgcc tcccccaccg tgcgcagcgc
     3121 gaccagctcc tcgtcgctca tggtgatgta gacgccgcgc tcggccagcg cccgctcgac
     3181 gctctcgatg acctccacga ggtcgagcga gtcggcgtgc aggtcctcgt cgaagtgcga
     3241 ggcctcgtcg agctcctcgt cgcccagccc cagcacggac gacaaccgat ccatcagcag
     3301 cgcgaggatc tcctcgcgcg tgaccacgtc gctcatgtcc cgcctccctc gtccacggca
     3361 cccgacatcg cgtccgcgac gcgccccgcc agatcctgcc cggccacgcg cgccgccgtg
     3421 cgcagcgcgg cggcgatctc ggcctcgccg gcggcgccgt gcgccaccag cacgttgccg
     3481 gtcacgccca ggagcaccgc cgcgccgccg tgctcgtcgt ctccgcccag cgcctccacc
     3541 gtcttcagga acacgttgcc ggtgaagccg tcggccacca ccacgtccac ggcgcccgcc
     3601 acgacggcgt ccggctcgac gttgccgccg aaagccgggc cgagcgccgc ggcgagcagt
     3661 tcgtgggcct cgcgggccag cgcgttgccc ttgcccgcct cggcgccgat gttgagcagc
     3721 ccgacgcggg gcgcggccac gcccaggacc tgcgcgtacg cgacacccat gcgggcgtgg
     3781 cccaccagca gctccgcggt cacgtccgcc gacgccccgg cgtcgaccag gacggtgccc
     3841 gtttcgccga agggcagcag cgcggcgacc gccggacggc gcacgccggg gagccggccg
     3901 agggcgaaca ggccggcgcc acgttggccg ccggcc
//
LOCUS       DATMKC010000169         4748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_218466, whole genome shotgun sequence.
ACCESSION   DATMKC010000169 DATMKC010000000
VERSION     DATMKC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218466"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1217
                     /locus_tag="VNU01_05005"
     CDS             99..1217
                     /locus_tag="VNU01_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH32010.1"
                     /translation="MSRPLHPSFAIRLERCADEMAPEHVRTAMRLFLACHREGRDPRE
                     EELHALLRDAQPAPSVEGLLQACHEGGEALWEAAVAEAGPPEQAELVDLARGLLHWQG
                     RLARTLVRDAPRLDREPQAARRALAGALLGAAAAPAVEERGIAGLAERAGVRLPERAE
                     VLAYRLQDGAGLPPDIAAVRHALEEAAGEPVLSEEDAVEGAVLLPAGDGAAERAVAAL
                     GRALGRPVTGGAAIAEGVGAIAAAAARAREVRDLAVALGRAPGVHRARQLLVELALSA
                     SPERLEELGRLLDPLARRPNLLATLEAWFAHDFDRRATAEALSIHRNTLDYRLRRVGD
                     LTGLALDSARGLQAAGAALVSRRLRASGKPTAGRLTGS"
     gene            complement(1214..3109)
                     /locus_tag="VNU01_05010"
     CDS             complement(1214..3109)
                     /locus_tag="VNU01_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32011.1"
                     /translation="MSMTPVNPFGGMSPGIAAVRRGAPEVGGRRLSRELLARVWRQFV
                     QPYRRRLALFLVAILVSAGLAVIPPVLFGMLIDHAVLDGDLGRVNVLALAAVAVAITA
                     ALFSLLQRYLSAWLGERIILDLRRTLYDHVQRMPLAFFTRTQTGALISRMNNDVIGAQ
                     QAVTGTFGSVVANVVQLLAALAVMVRLEWRLTLLVLAVLPLFVAPARRVGRKLQDITR
                     EQMVVNAQMNSRMTERFNVAGALLVKLFGRYREEAEEFGSRAQRVAEIGVRGAVLGRI
                     FFVTLALVGAVGTAVVYWLGGRMAITSPDFSPGNIVTFAALVTQAYTPLSALTNARVE
                     IMTAFVSFERVFEVLDVRHPIADRDDAVELADPAGEVVFDDVTFAYPSAAESSLLSLE
                     DSGAPLDERPGELVLHGVSFTARPGKTVALVGPSGAGKSTICSLIPRLYDVTTGAVRV
                     DGLDVRDLMGDSLRAAIGVVPQDPHLFHETVAANLRYARPDATPDDLEAACRAAQIHD
                     VIAALPDRYDTLVGERGYRLSGGEKQRLAIARLLLKDPAIVILDEATAHLDSENEALV
                     QEALRTALAGRTSIVIAHRLSTIVAADQILVVQAGRVVQRGRHEELFRAEGLYAELAR
                     TQFARSG"
     gene            complement(3106..>4748)
                     /locus_tag="VNU01_05015"
     CDS             complement(3106..>4748)
                     /locus_tag="VNU01_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWH32012.1"
                     /translation="HERFEHLGGYAVESEARQVLAGLGFSETEMERPPTVLSGGWMMR
                     VALARLLLSDPDVLLLDEPTNHLDLDSVTWLQGFLAEYSGVLVLVSHDRDFVNAAVNR
                     VVDLANQTATEYVGDYEAFVQQRALRAEQLASAAAGQARQIAATEAFIERFRYKASKA
                     RQVQSRIKQLDKLDRVVVPQSRTKALKFRFPPPPRSGREVLRLDGVVKRYGDLTVYDG
                     LDLSLERGQKIALVGPNGAGKSTLLKILAGVLDIDGGERVLGHNVRVAYYAQHQLEAL
                     EPERTVLEEFATAVPPGSGVNPRTMLGSFLFSGEDVDKRVAVLSGGERARLALAKLLV
                     EPANLLCMDEPTNHLDITSRDVLEDALREYAGTVVLISHDSYLIRSLADTIVEVRDGA
                     AHVHLGDYEAYLERMPDPTGRDHLARPEPFTAAPVAPGPTPDATASSASAPRVRKTAE
                     QKRQEAELRNRLHRETKALRRRQEELEAQLAEAEAQAAECEKLLAEPDVYEDGERVRE
                     LVLRHRLADDRVATLTAEWERVSTELESATERATMGST"
BASE COUNT          623 a         1763 c         1753 g          609 t
ORIGIN      
        1 tttcgggacc ttgccaatag aagcggccga gctcgatccc gacaatccgg cgagggcccg
       61 atgcccgcgc aatgcctgtc gtcccaacgg gggaacgtgt gagccggccg cttcatccca
      121 gcttcgccat ccggctggag cggtgtgcgg acgagatggc ccccgagcac gtccgcaccg
      181 cgatgcggct gttcctcgcg tgccaccgcg aggggcgcga cccgcgcgag gaggaactgc
      241 acgcgctgct gcgcgacgcc cagccggctc cctccgtgga gggcctgctg caggcgtgcc
      301 acgagggcgg cgaggcactg tgggaggcgg cggtcgccga agccgggccg ccggagcagg
      361 ccgagctcgt ggacctggcg cgcggcctgc tgcactggca agggcgcctg gcccggacgc
      421 tcgtccgcga cgcgccccgg ctggaccggg agccacaggc cgcgcggcgg gcgctggccg
      481 gcgccctgct gggggccgcc gcggcccccg ccgtggagga gcgcggcatc gcgggcctgg
      541 ccgagcgcgc cggcgtgcgc ctgcccgagc gcgccgaggt gctggcctac cgcctccagg
      601 acggggccgg cctgcccccg gacatcgccg ccgtccggca cgcgctcgag gaggccgccg
      661 gcgagccggt gctcagcgag gaggacgccg tcgagggcgc cgtcctgctg ccggccggcg
      721 acggcgccgc cgagcgcgcg gtcgcggcgc tgggccgcgc gctgggacgg cccgtcaccg
      781 ggggtgccgc catcgcggag ggcgtcgggg ccatcgccgc ggccgccgca cgcgcccgcg
      841 aggtccgcga cctggccgtc gcgctgggcc gcgcgccggg ggtccaccgc gcgcggcagc
      901 tgctggtgga gctggcgctg agcgccagcc cggagcggct ggaggaactg ggccggctgc
      961 tggacccctt ggcgcggcgg ccgaacctgc tggcgaccct ggaggcgtgg ttcgcccacg
     1021 acttcgaccg gcgcgccacg gcggaggcgc tgtcgatcca ccgcaacacg ctggactacc
     1081 gcctccggcg cgtcggtgac ctcacgggcc tggcgctgga ctcggcgcgc gggctgcagg
     1141 ccgccggcgc ggccctggtc tcgcggcgcc tgcgcgcatc ggggaagccg acggccgggc
     1201 gcctgaccgg gagctagccg gagcgggcga actgcgtgcg cgccagctcc gcgtacagcc
     1261 cctcggcccg gaacagctcc tcgtggcggc cccgctggac cacgcggccg gcctgcacga
     1321 ccaggatctg gtcggccgcc acgatcgtgg acaggcggtg ggcgatgacg atcgacgtgc
     1381 gcccggccag ggccgtgcgc agcgcctcct gcaccaaggc ctcgttctcc gagtccaggt
     1441 gcgccgtcgc ctcgtccagg atcacgatcg cggggtcctt gagcagcagg cgggcgatcg
     1501 cgaggcgctg cttctcgccg ccggacaggc ggtagccgcg ctcccccacc agcgtgtcgt
     1561 agcggtcggg cagcgcggcg atcacgtcgt ggatctgcgc ggcccggcag gcggcctcca
     1621 ggtcgtccgg cgtggcgtcc gggcgcgcgt agcgcaggtt cgccgcgacg gtctcgtgga
     1681 acaggtgcgg gtcctgcggc accacgccga tggcggcgcg cagcgagtcg cccatgaggt
     1741 cgcgcacgtc cagcccgtcg acgcgcaccg ccccggtggt gacgtcgtac agccgcggga
     1801 tcagcgagca gatggtcgac ttgccggcgc ccgacggccc caccagcgcg acggtcttgc
     1861 cgggccgcgc ggtgaagctc acgccgtgca gcaccagctc gccggggcgc tcgtccagcg
     1921 gcgcgccgct gtcctccagc gacagcaggg aggactcggc cgccgacggg taggcgaacg
     1981 tgacgtcgtc gaacaccacc tcgccggccg ggtccgccag ctcgacggcg tcgtcgcggt
     2041 cggcgatcgg gtggcgcacg tcgagaacct cgaacacgcg ctcgaagctc acgaacgccg
     2101 tcatgatctc cacgcgcgcg ttcgtcagcg ccgacagcgg cgtgtacgcc tgcgtcacca
     2161 gcgccgcgaa cgtgacgatg ttgcccggcg agaagtccgg cgacgtgatg gccatgcgcc
     2221 cgcccagcca gtacacgacg gcggtgccca cggcgcccac cagcgccagc gtgacgaaga
     2281 agatgcgtcc cagcacggcg ccgcgcacgc cgatctcggc gacgcgctgc gcgcgggacc
     2341 cgaactcctc cgcctcctcg cggtagcgcc cgaacagctt cacgagcagg gcgccggcca
     2401 cgttgaagcg ctcggtcatg cgcgagttca tctgcgcgtt gacgaccatc tgctcgcgcg
     2461 tgatgtcctg cagcttccgg ccgacacgcc gcgcgggcgc gacgaacagc ggcagcaccg
     2521 ccaggaccag cagggtgagc cgccattcca gccgcaccat gacggcgagc gccgccagca
     2581 gctgcaccac gttggcgacc acggacccga acgtgccggt gacggcctgc tgggcgccga
     2641 tgacgtcgtt gttcatccgg ctgatcagcg cgccggtctg tgtgcgcgtg aagaacgcca
     2701 gcggcatgcg ctgcacgtgg tcgtagagcg tgcgccgcag gtccaggatg atgcgctcgc
     2761 cgagccaggc cgacaggtag cgctgcagca gcgagaacag ggcggcggtg atcgccacgg
     2821 cgaccgccgc caaggccagc acgttgaccc ttcccaggtc gccgtcgagc acggcgtggt
     2881 cgatcagcat gccgaacagc accggcggga tcaccgccag gccggccgag accaggatgg
     2941 cgaccaggaa cagcgccagg cggcggcggt acggctggac gaactgccgc cacacccgcg
     3001 ccagcagctc gcgcgacagc cgccgcccgc cgacctcggg ggcgccgcgg cgcacggcgg
     3061 cgatgccggg gctcatgccc ccgaaggggt tgacgggcgt catgctcatg tggagcccat
     3121 cgtcgcacgc tccgtggcgg actcgagctc cgtggagacc cgctcccact cggcggtgag
     3181 cgtggcgacg cggtcgtcgg ccaggcggtg gcgcagcacc agctcccgca cgcgctcgcc
     3241 gtcctcgtag acgtccggct cggccagcag cttctcgcac tcggcggcct gcgcctccgc
     3301 ctcggcgagc tgcgcctcca gctcctcctg gcgccgccgc agcgccttgg tctcgcggtg
     3361 cagccggttg cgcaactccg cctcttgcct tttctgctcc gcggtcttgc gcaccctcgg
     3421 cgcactcgca gaggacgcgg tcgcgtcggg ggtagggccg ggggcaaccg gcgcggccgt
     3481 aaagggctct ggtcgcgcca ggtggtcccg gcccgtcgga tccggcatac gttcgaggta
     3541 cgcctcgtag tcgccgaggt gtacgtgcgc ggcgccgtcg cgcacctcga cgatggtgtc
     3601 cgccagcgag cggatcaggt agctgtcgtg ggagatcagc accacggtgc cggcgtactc
     3661 gcgcagggcg tcctccagga cgtcgcggct ggtgatgtcc aggtggttgg tcggctcgtc
     3721 catgcacagc aggttggccg gctccaccag gagcttcgcc agcgccaggc gggcgcgctc
     3781 gccgccggac agcaccgcga cgcgcttgtc gacgtcctcg ccgctgaaca ggaacgaccc
     3841 cagcatggtg cgcgggttca cgccgctgcc cggcggcacc gcggtggcga actcctccag
     3901 caccgtgcgc tccggctcca gcgcctccag ctggtgctgg gcgtagtagg cgacgcgcac
     3961 gttgtggccc aggacgcgct cgccgccgtc gatgtccagc acgcccgcca ggatcttcag
     4021 cagcgtggac ttgccggcgc cgtttggccc caccagcgcg atcttctggc cgcgctccag
     4081 gctcaggtcc aggccgtcgt agacggtcag gtcgccgtag cgcttgacca ccccgtccag
     4141 gcgcagcacc tcgcgcccgg agcgcggcgg gggcgggaag cggaacttga gcgccttggt
     4201 gcggctctgc ggcaccacca cgcggtccag cttgtcgagc tgcttgatgc ggctctgcac
     4261 ctggcgcgcc ttggacgcct tgtagcggaa gcgctcgatg aacgcctccg tggcggcgat
     4321 ctgccgggcc tgcccggcgg cggccgaggc gagctgctcg gcgcgcaggg cgcgctgctg
     4381 gacgaacgcc tcgtagtcgc cgacgtactc cgtcgcggtc tggttcgcca ggtccacgac
     4441 gcggttcacc gcggcgttga cgaagtcgcg gtcgtggctg accaggacca gcacgccgga
     4501 gtactccgcc aggaagccct gcagccaggt gacgctgtcc aggtccagat ggttggtcgg
     4561 ctcgtccagc agcagcacgt cggggtcgct cagcagcagc cgggccagcg ccacgcgcat
     4621 catccagccg ccggacagca ccgtgggcgg gcgctccatc tcggtctcgg agaaacccag
     4681 gccggccagc acctggcggg cctcggactc caccgcgtag ccgcccaggt gctcgaagcg
     4741 ctcgtgca
//
LOCUS       DATMKC010000170         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_168775, whole genome shotgun sequence.
ACCESSION   DATMKC010000170 DATMKC010000000
VERSION     DATMKC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168775"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VNU01_05020"
     CDS             complement(<1..440)
                     /locus_tag="VNU01_05020"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWH32013.1"
                     /translation="MDLMEYQGKNLFRRYGIPTTPQGEIATTPEEAQRLAEGFGVPVM
                     VKAQVLTGGRGKAGGVKYCPDAAAARENAERILGLDIKGHVVKQVLVEPASDIAEEYY
                     LSILHDRVSKGYKVIASPEGGVDIEETNRTNPEKVAKVDIDPTVG"
     gene            complement(468..863)
                     /locus_tag="VNU01_05025"
     CDS             complement(468..863)
                     /locus_tag="VNU01_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWH32014.1"
                     /translation="MSIRVLIAKPGLDGHDRGAKIVARALRDGGMEVVYTGLHQTPEQ
                     IVEAALQEDVQCVGMSIHSGAHMTLFPKVVALLRERGADDVVVFGGGIIPADDITALK
                     QAGVAEIFTPGARTTDIVEWVQANVRPRD"
     gene            1037..2470
                     /locus_tag="VNU01_05030"
     CDS             1037..2470
                     /locus_tag="VNU01_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWH32015.1"
                     /translation="MTERLRAQQWAELLLEPGAEALAGPAVTANPLDWPGYDDLRADA
                     PGDEAVRAWAGRAGGLPLVLLAFDFAHLGGSMGAGVGARVAHAFAEAARRRLPVVTVT
                     ATGGARMHEGMVSLAQMTRTTLAARAHRDAGLLQVTVAADPTTGGVYVSFAAQADLVV
                     AEPGAYVAFAGPRVAASMGGSAEAADTNRAESALAHGLVDAVVPRAELRAWVARALGA
                     VVPAVPVPVAPEPVEPAGGDAWARVLAARSDARPRAQAYLGHFTDVTRLSGDRAGGRD
                     DGVVCALARLHGRPLGLVALDRQPVTPAGYRTAWRLLSTAERLGLPVLTLVDTPGATP
                     AREAEVAGQARAIAETFDRLAALRTPTVCVITGEGGSGGALALASADRLLMQRGAVFS
                     VIAPEGAAAILHRDAERAPEVAERLQPTAERLVALGIAHGLVPDDPAGALAHAAAALD
                     ELAGLGPDQLLARRHAHWMAVGGGLGS"
     gene            2467..>2740
                     /locus_tag="VNU01_05035"
     CDS             2467..>2740
                     /locus_tag="VNU01_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32016.1"
                     /translation="MSGPGDPADGGTDRPPEFGPRGYVPPQAAKRARKIVLREPMGLG
                     WPVAAVVAGVLLVLLGVVFLLTRTGPPGAPFEPVAEVADVDPRGAVV"
BASE COUNT          288 a         1005 c         1081 g          366 t
ORIGIN      
        1 cccacggtcg ggtcgatgtc gaccttggcg accttctccg ggttggtgcg gttggtctcc
       61 tcgatgtcga cgccgccttc gggcgaggcg atcaccttgt agcccttgga gacccggtcg
      121 tgcaggatcg acaggtagta ctcctcggcg atgtcgctgg ccggctccac cagcacctgc
      181 ttgaccacgt ggcccttgat gtccaggccc aggatgcgct cggcgttctc acgcgccgcg
      241 gcggcgtcgg ggcagtactt caccccgccg gccttgccgc gcccgcccgt gaggacctgc
      301 gccttcacca tcaccggcac gccgaagccc tcggccaggc gctgcgcctc ctccggcgtg
      361 gtggcgatct cgccctgcgg ggtggtcggg atgccgtagc ggcggaacag gttcttgccc
      421 tggtactcca tcaagtccat ggagcgcggc ccttcctctc gtgtggtcta gtcgcgcggg
      481 cgcacgttgg cctgtaccca ctcgacgatg tcggtggtgc gcgcaccggg cgtgaagatc
      541 tccgccacgc cggcttgctt cagggcggtg atgtcgtcgg ccgggatgat ccccccgccg
      601 aagaccacga cgtcgtccgc gccgcgctcg cgcagcagcg ccacgacctt ggggaacagc
      661 gtcatgtggg cgccggagtg gatggacatg cccacgcact ggacgtcctc ctgcagcgcc
      721 gcctccacga tctgctcggg cgtctggtgc aggccggtgt agacgacctc catcccgccg
      781 tcgcggaggg cgcgggcgac gatcttcgcc ccgcggtcgt ggccgtcgag gcccggcttc
      841 gcgatcagca cgcggatgga cacggttttg cgcctcctgg tgtgcgcgga acagactgcg
      901 cagtggcacc tggcaccgcg cgcgcaccag tgcggcgcac ggcggcggaa tggcgcggat
      961 cgtagccgac ccgcgaaaac gtccgccaac cggccagatt gccgtcagcc cccgttcacg
     1021 tgtcaggagc ccgccggtga ccgaacgact gcgcgcgcag cagtgggccg agctgctgct
     1081 ggagcccggc gccgaggcgc tggccggccc cgcggtcacg gccaacccgc tggactggcc
     1141 gggctacgac gacctgcgcg ccgatgcacc gggggacgag gcggtgcgcg cctgggcggg
     1201 gcgcgcgggc ggcctgcccc tggtgctgct ggcgttcgac ttcgcccacc tgggcggctc
     1261 gatgggcgcc ggcgtgggcg cgcgcgtcgc gcacgccttc gcggaggcgg cgcggcggcg
     1321 gctgccggtg gtgacggtga cggccaccgg cggcgcgcgc atgcacgagg gcatggtgtc
     1381 cctggcgcag atgacgcgca ccacgctggc ggcgcgggcg caccgcgacg cggggctgct
     1441 gcaggtcacc gtcgcggccg accccaccac ggggggcgtg tacgtgtcgt tcgccgcgca
     1501 ggcggacctc gtcgtggcgg agcccggcgc ctacgtggcc ttcgcggggc cgcgcgtggc
     1561 cgccagcatg ggcgggtccg ccgaggcggc cgacacgaac cgcgccgagt ccgcgctggc
     1621 gcacggcctg gtcgacgccg tggtgccgcg cgcggagctg cgcgcatggg tggcgcgggc
     1681 gctgggggcc gtggtgcccg ccgtgccggt gccggtggcc cccgagccgg tggagccggc
     1741 gggaggcgac gcctgggcac gcgtgctcgc cgcgcggtcc gacgcgcgcc cgcgcgcgca
     1801 ggcctacctc ggccacttca ccgacgtcac gcggctgtcc ggcgaccgcg ccggcggccg
     1861 cgacgacggc gtggtctgcg cgctcgcccg gctgcacggc cggccgctgg gcctggtggc
     1921 gctggaccgg cagccggtga cgcccgccgg ctaccgcacg gcgtggcggc tgctgtccac
     1981 cgcggagcgc ctgggcctgc cggtgctcac gctggtggac acgcccggcg ccacaccggc
     2041 gcgcgaggcg gaggtcgcgg gccaggcgcg cgcgatcgcc gagacgttcg accgcctggc
     2101 cgcgctgcgc acccccaccg tgtgcgtgat cacgggcgaa ggcggctccg gcggcgcgct
     2161 ggcgctggcg tcggccgacc ggctgctgat gcagcggggc gccgtgttca gcgtcatcgc
     2221 cccggagggc gcggcggcga tcctgcaccg cgacgcggaa cgggcaccgg aggtcgccga
     2281 gcggctgcag cccaccgccg agcggctggt ggcgctgggc atcgcgcacg ggctggtgcc
     2341 cgacgacccg gcgggcgcgc tggcgcacgc ggccgccgcc ctggacgagc tggccgggct
     2401 gggcccggac cagctgctgg cgcggcgcca cgcccactgg atggcggtcg gcgggggtct
     2461 gggctcgtga gcgggccagg cgacccggcc gacggcggca ccgaccggcc accggagttc
     2521 ggcccgcgcg gctacgtgcc cccgcaggcg gccaagcgcg cccgcaagat cgtgctgcgc
     2581 gagccgatgg ggctcggctg gccggtcgcc gcggtggtgg cgggcgtcct gctggtgctg
     2641 ctgggcgtgg tgttcctgct gacgcggacg ggtcctcccg gtgcgccgtt cgagccggtc
     2701 gccgaagtgg cggacgtcga cccgcggggg gcggtcgtgg
//
LOCUS       DATMKC010000171         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_254715, whole genome shotgun sequence.
ACCESSION   DATMKC010000171 DATMKC010000000
VERSION     DATMKC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254715"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VNU01_05040"
     CDS             complement(<1..215)
                     /locus_tag="VNU01_05040"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWH32017.1"
                     /translation="MIALVQRVSRAHVEVGGATIGEIAAGLLVLVCAERGDTEAHADK
                     LLAKVLKLRIFGDEAGKMNRSVQDVNGG"
     gene            complement(212..1126)
                     /locus_tag="VNU01_05045"
     CDS             complement(212..1126)
                     /locus_tag="VNU01_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWH32018.1"
                     /translation="MSAAWVRSLSPQLLLRASIAVALATIALKFLAWWITNSVGLLSD
                     AMESFVNLAGASFALLMVTIARRPADADHPYGHHKAEYFSSGFEGLAIIVAAVGILWA
                     AVGRIFDPQPLDQLGPGLALSVLSSVVNGALAWAMLASGRAHRSIALEGGGRHLLTDV
                     WTSAGVVVGVGLVAVTGWQWLDPAVAIAVALNILREGVSLMWRSSQGLMDEALEPEVM
                     AVVQKTLDGFRHPTIRFDHVSSRRSGQRRFVDLHMHMPGSWSLRRAAAVRAEVEQALM
                     SAVPGLRATIQLLPTDVEAHTADEEDPI"
     gene            complement(1180..1587)
                     /locus_tag="VNU01_05050"
     CDS             complement(1180..1587)
                     /locus_tag="VNU01_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HWH32019.1"
                     /translation="MLLLDSSVWIDSMLGRVTDATRFVESRDEGEEIATTGTVFQEVL
                     QGIRSPREYDRMRQVLWSLLVLEPRELSTYEVAAQLYVRARQKGFTVRKPNDCLIAAL
                     ALEHGALLVHNDRDFLALAQVEPALLVYPGRPH"
     gene            complement(1587..1880)
                     /locus_tag="VNU01_05055"
     CDS             complement(1587..1880)
                     /locus_tag="VNU01_05055"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWH32020.1"
                     /translation="MLTNIDIDENLIAEAMKVSGARTKREVVDRALRDMVARSKRPRL
                     RDIWGMAGPDTFWPGYDPKTDPGEPPGKYRVEQGLASYKVPPPPATPRARKKR"
     gene            1879..>2525
                     /locus_tag="VNU01_05060"
     CDS             1879..>2525
                     /locus_tag="VNU01_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWH32021.1"
                     /translation="MESLYMPCTFRQRVLWLLLLLGPPLASGQVPPTPAEVRAFTGLH
                     AAAHLGHVDAIRTLRAAGADPNARDGHGRTPLHVATFARQRDAIRALAQAGADLNLLE
                     NDRYDAVTIASVANDEETLRLLLSLGASAKQVTSRYDGTALIAAAHLGHAGVVRQLIA
                     AGAPLDHVNNLHWTAAIEAVVLGDGGPRHQETLRALVDAGANLRLADRNGATPLQL"
BASE COUNT          371 a          921 c          885 g          348 t
ORIGIN      
        1 ccgccgttca cgtcctgcac gctgcggttc atcttgccgg cctcgtcgcc gaaaatgcgc
       61 agcttcagga ccttggccag cagcttgtcg gcgtgcgcct cggtgtcgcc gcgctcggcg
      121 cagaccagca ccagcagccc ggccgcgatc tcgccgatcg tcgcgccgcc gacttccacg
      181 tgcgcgcggc tcacgcgctg gaccagggcg atcaaatcgg gtcctcctcg tccgccgtgt
      241 gcgcctccac gtccgtcggc agcagctgga tcgtggcgcg caggcccggc acggcgctca
      301 tgagggcctg ctcgacttcg gcccgcacgg cggcggcgcg gcgcaggctc cagctgcccg
      361 gcatgtgcat gtgcaggtcg acgaagcggc gctggcccga cctgcggctg gacacgtggt
      421 cgaagcggat cgtggggtgg cgaaagccgt ccagcgtctt ttgcaccacc gccatgacct
      481 cgggctccag cgcctcgtcc atgaggccct gcgacgagcg ccacatcagc gacacgcctt
      541 cgcgcaggat gttgagcgcg acggcgatcg ccaccgccgg gtccagccac tgccagccgg
      601 tgaccgcgac caggcccacg ccgacgacca cgccggccga ggtccacacg tccgtgagca
      661 ggtggcggcc gccgccctcc agcgcgatcg agcggtgcgc ccggcccgag gccagcatcg
      721 cccaggccag cgcgccgttc acgacggaac tcaggaccga cagcgccagg ccggggccga
      781 gttggtcgag cggctgcgga tcgaagatgc gccccacggc ggcccacagg atgccgaccg
      841 cggcgacgat gatcgccagg ccttcgaagc cggacgagaa gtactcggcc ttgtgatggc
      901 cgtagggatg gtccgcgtcc gccggccggc gcgcgatggt gaccatcagc agggcgaagc
      961 tcgcgccggc gaggttcacg aaggactcca tcgcgtccga cagcaggccg acggagttgg
     1021 tgatccacca cgcgaggaac ttcagcgcaa tggtggccag cgcgaccgcg atggaggcgc
     1081 gcagcagcaa ctgcggcgac agcgagcgaa cccacgcggc ggacatggcg gcattctgct
     1141 cgattcgcca gcggccgcga gccgcgcggc gcggccgcct cagtgcggcc gccccgggta
     1201 gaccagcagc gcgggctcga cctgcgccag cgcgaggaag tcgcggtcgt tgtggacgag
     1261 gagcgcgccg tgctccagcg ccagcgccgc gatgaggcag tcgttgggct tgcgtacggt
     1321 gaagcccttt tgccgcgccc gcacatacag ctgcgccgcc acctcgtacg tggagagctc
     1381 gcgcggctcc aggacgagca gcgaccacag cacctgccgc attcggtcgt actccctggg
     1441 gctgcggatg ccctggagca cctcctggaa cacggtgccc gtcgtcgcga tctcttcgcc
     1501 ctcgtcgcgc gactcgacga accgggtggc gtccgtcacg cggccgagca tggaatcgat
     1561 ccacaccgag gagtcgagca gcagcatcag cgcttcttcc tcgctctggg cgtcgccggg
     1621 ggcggcggga ccttgtacga ggccaggccc tgttccacgc ggtacttgcc cggcggctcg
     1681 ccgggatcgg tcttggggtc gtagccgggc cagaacgtgt ccggaccggc catgccccag
     1741 atgtcgcgca agcgcgggcg cttggaccgt gccaccatgt cgcgcagggc gcggtcgacg
     1801 acctcacgct tggtccgcgc gccggaaacc ttcatggcct ccgcgatgag gttctcgtcg
     1861 atgtcgatgt tggtgagcat ggagtcattg tatatgcctt gtacattccg tcaacgggtc
     1921 ctgtggctcc tcctgctgct cggcccgccg ctggcttcgg gccaggtgcc gcccacgccc
     1981 gccgaggtgc gggccttcac gggccttcac gcggcggcac acctcggcca cgtggacgcg
     2041 atccgcacgc tgcgcgccgc cggcgcggac ccgaacgcgc gcgacggcca cggccgcacg
     2101 ccgctgcatg tggcgacctt cgccaggcag cgcgatgcga tccgggcact cgcgcaggcg
     2161 ggcgccgacc tgaacctgct ggagaacgac cgctacgacg cggtgacgat cgcctcggtc
     2221 gcgaacgacg aggagacgct gcggctgctc ctgtcgctcg gcgccagcgc gaagcaggtg
     2281 accagccgct acgacggcac ggccctcatc gccgccgcgc acctcggtca cgccggcgtg
     2341 gtccgccaat tgatcgcggc cggcgcaccg ctggaccacg tgaacaacct gcactggacg
     2401 gccgcgatcg aggccgtggt gctcggcgac ggcggaccgc gccaccagga gacgctgcgg
     2461 gcgctggttg acgcgggcgc caacctgcgg ctcgccgaca ggaacggcgc gacgccgctg
     2521 cagct
//
LOCUS       DATMKC010000172         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_248017, whole genome shotgun sequence.
ACCESSION   DATMKC010000172 DATMKC010000000
VERSION     DATMKC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248017"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..145
                     /locus_tag="VNU01_05065"
     CDS             8..145
                     /locus_tag="VNU01_05065"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWH32022.1"
                     /translation="MCEKMLSYFIHKVSPGRWVSKPRIVICVPSGITGVEQRAVRDAA
                     R"
     gene            257..1309
                     /locus_tag="VNU01_05070"
     CDS             257..1309
                     /locus_tag="VNU01_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWH32023.1"
                     /translation="MSQSFSSAFQFFGRDIAVDLGTANTLVYVRGRGIVLNEPSVVAI
                     NTKNGAILAVGSEAKRMIGRTPGHIVAIRPLKDGVIADFDVTEKMLRYFIQKVHRRRH
                     LTMLNKPRVVVCVPSGITGVEQRAVEEASIQAGARSAYIIEEPMAAAIGAGLPVHEPA
                     GNMIVDIGGGTTEVAVISLGGIVTSASIRIGGDELDESIINYVKKEYSLMLGERTSEE
                     IKMAIGSAFPLPDENHAEIRGRDLVSGLPKTIIVSAEEIRRAIEEPVNSIIDAVKNTL
                     DRTPPELAADIMDKGIVLTGGGALLQGLDERLKHETGMPIHVAENPLHSVAIGSGKCL
                     EEFEALKRVLISSSRH"
     gene            1351..>2128
                     /gene="mreC"
                     /locus_tag="VNU01_05075"
     CDS             1351..>2128
                     /gene="mreC"
                     /locus_tag="VNU01_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWH32024.1"
                     /translation="MSSRRSRTLVVALAVAALALLTLDYRQGDGGIVAAMQRGVTAVL
                     APVQEGLSDVVRPVGDFFEAIAELDDLRQQNAELEAELQDLREGKFSRADLERENTEL
                     RALLGMRERLGYTTTGATVIAQPPNAFEWSVLIDAGAEQGIEPGMAVINAEGLVGKVI
                     DVTRRNARVQLLTSPNAGYAVRVSGTGEEGLLTGRGSRPFQLEVLNPEAEIPAGSQVV
                     TRVFQGTTIPDGIPIGETEQARAGGSRFVYVRPYVDFTRLS"
BASE COUNT          334 a          762 c          726 g          306 t
ORIGIN      
        1 cttcgacatc tgcgagaaga tgctgtccta cttcatccac aaggtcagcc ccggccggtg
       61 ggtgtccaag ccccgcatcg tgatctgcgt gccgtcgggc atcacgggcg tggagcagcg
      121 ggccgtgcgt gacgccgccc ggtgaccggg tgtacgctcg gccacgtcgg tctccgcgac
      181 gacctcgtta gactcgcccg atctgtcgat ctggccgcgt ccggcgtcag gtccgccttc
      241 ccccgcagga gcattggtgt cccagagctt ctccagtgcg ttccagttct tcggccgcga
      301 catcgcggtc gatttgggca ccgcgaacac cctggtctac gtgcgcgggc gcggcatcgt
      361 gctgaacgag ccgtccgtgg tggcgatcaa caccaagaac ggcgcgatcc tggcggtggg
      421 ctccgaggcc aagcgcatga tcgggcgcac ccccggccac atcgtcgcca tccggccgct
      481 gaaggacggc gtcatcgccg acttcgacgt gaccgagaag atgctgcgct acttcatcca
      541 gaaggtgcat cgccgccgcc acctcaccat gctgaacaag ccgcgcgtgg tggtctgtgt
      601 gccgtccggc atcaccggcg tggagcagcg cgccgtcgag gaggcctcga tccaggcggg
      661 cgcccgctcc gcctacatca tcgaggagcc catggccgcc gcgatcggcg ccgggctgcc
      721 ggtgcacgag ccggccggca acatgatcgt cgacatcggc ggcggcacca ccgaggtcgc
      781 cgtgatctcc ctgggcggca tcgtcaccag cgcctccatc cgcatcggcg gggacgagct
      841 ggacgagtcg atcatcaact acgtgaagaa ggagtactcc ctcatgctgg gggagcgcac
      901 ctcggaagag atcaagatgg ccatcggcag cgcgttcccg ctgcccgacg agaaccacgc
      961 cgagatccgc ggccgcgacc tcgtctccgg cctgcccaag accatcatcg tgagcgccga
     1021 ggagatccgc cgcgccatcg aggagccggt caactcgatc atcgacgccg tgaagaacac
     1081 cctggaccgc acgcccccgg agctggccgc cgacatcatg gacaagggca tcgtgctcac
     1141 cggaggcggc gcgctgctgc agggcctgga cgagcgcctc aagcacgaga ccggcatgcc
     1201 catccacgtc gcggagaacc cgctgcactc ggtggccatc ggctccggca agtgcctgga
     1261 ggagttcgag gccctcaagc gggtgctcat ctcctcgtcg cggcactgac cctccgctcc
     1321 cgcggcgggc ccgcccacta ggctccagca atgtcctcgc gtcgcagccg aacccttgtc
     1381 gtggccttgg ccgtggcggc cctcgcgctg ctgacgctcg actaccgcca gggcgacggc
     1441 ggcatcgtgg ccgcgatgca gcggggggtg accgccgtgc tggcgccggt gcaggagggc
     1501 ttgagcgacg tcgtccggcc cgtcggggac ttcttcgagg ccatcgccga gctcgacgac
     1561 ctgcgtcagc agaacgccga gctggaggcg gagctgcagg atctgcgcga gggcaagttc
     1621 tcgcgcgccg acctcgagcg cgagaacacc gagctgcgtg cgctgctcgg catgcgcgag
     1681 cggctgggct acaccaccac gggcgccacc gtcatcgcgc agccgcccaa cgcgttcgag
     1741 tggagcgtgc tgatcgacgc cggggccgag cagggcatcg agccgggcat ggccgtgatc
     1801 aacgcggagg gcctggtcgg caaggtcatc gacgtcacgc gccgcaacgc gcgcgtccag
     1861 ctgctgacca gccccaacgc cggctacgcg gtgcgcgtca gcggcaccgg cgaggagggg
     1921 ctgctcaccg ggcgcggctc gcggccgttc cagctggagg tgctcaaccc ggaggcggag
     1981 atccccgccg gatcgcaggt ggtgacccgg gtgttccagg gcaccacgat ccccgacggc
     2041 atccccatcg gcgagaccga gcaggcgcgc gccggcggca gccgcttcgt ctacgtgcgg
     2101 ccctacgtgg acttcacgcg cctgagcc
//
LOCUS       DATMKC010000173         3832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_35672, whole genome shotgun sequence.
ACCESSION   DATMKC010000173 DATMKC010000000
VERSION     DATMKC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35672"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VNU01_05080"
     CDS             <1..298
                     /locus_tag="VNU01_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32025.1"
                     /translation="VTNRHYTEVLTPLIKLAADRGELRADADHAQLLAYLLLLLPHLA
                     LAPFGPELDPALGLYGKDPEQLTAPVHGLVGALERAFGPVNHVPDQQRPERTAR"
     gene            295..2454
                     /locus_tag="VNU01_05085"
     CDS             295..2454
                     /locus_tag="VNU01_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32026.1"
                     /translation="MTAIDRTDTYCVIGAGSSGLTAAKNLREHGFAVDVYEREDDVGG
                     NWNIGAENSRVYHSTHLISSKPFTEYPDFPMPDEFPDYPHHSQMHAYFRAYMRHFGLD
                     EVIRFSTEVVSCEPVDGGRHWDVTVRPRGSKGTRKQEVFRYAGLVIANGHNWFPKIPD
                     YPGEFTGELMHSAAYKDPEVLRGKRVLVVGAGNTGCDIAVEAAQNAARTLHSTRRGYW
                     YSPKYSYGKPSDQVYDLMLSLKLPKPLLQRMMERTLKMSQGDITRVGLREPDHHILET
                     HPIVNSQLVYYVGHGEIDPKPDIERLDGDGVVFTDGTREEVDLIVWCTGYLVRFPFID
                     HEHLNWAGDHPKLFQNVFHPRYDNLFVIGLIQPDSGQFKLVHWQSVAVARYLESQRAQ
                     PAAAEAFRRHRDAHFDDPLSGGVHYKDSTRHYFEINHYSYLEGIQELLHVMEAERPSK
                     SKVMEPMRWAFPSSPVAREVVTREPSQPVDGRPPLLFVHGAGHGAWTWRNWMAAAAER
                     GWASWALSLRGHGDSGGHEDIRRWRLRDYEHDVMQTIASMPQPPVLVGHSMGGLVVQR
                     VLARYPARAGVLVAAVPPRHGIETTLAIARRHPQDLLKGLAGASLPWRKEYLFTPDTP
                     DEVALPVMHACTDESPRAQIETTGPHGAYPSKAPVLVLGAERDGLVPPSAVMRTARHY
                     GTKAHLYRGMGHDMMLDPHWRAPLDLMLDWLEKTLSA"
     gene            complement(2461..3240)
                     /locus_tag="VNU01_05090"
     CDS             complement(2461..3240)
                     /locus_tag="VNU01_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32027.1"
                     /translation="MTSPLGVMSVILLEAAVGSAVVLWAGGVWGRVRRGFFLLIGSTI
                     VLSAIGAWRLADGEFTAAPSPATDRAVLALAVFAGLALAWQVLLFVHQGLSRAVGLAA
                     TAAGVVALVLFGLARPHDPALAVLELALGAVFLGATNYGLLLGHWYLVERKLSKDPMV
                     RSAWWYVAGVVAGVGTAALSAANPSPEAGGLSLYFSVPGFSVLLAGGLVAVCALISGF
                     VVKLAKEGGRSIQAATGMFYLAVIMAFSAEMAAKVRYFATG"
     gene            3353..3742
                     /locus_tag="VNU01_05095"
     CDS             3353..3742
                     /locus_tag="VNU01_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HWH32028.1"
                     /translation="MSAHDDEGCLPGRRCRITLLVGEADTAIAMGSGDVPVLASPRVV
                     ALAEQASVEAIAGTLDEGKTSVGAWVELEHNAPSRVGETVTAEATLLGVHGRKLEFSV
                     SVRNEAGEEVAHVRHRRVVVTRAKFAG"
BASE COUNT          624 a         1451 c         1302 g          455 t
ORIGIN      
        1 cgtcaccaac cgccactaca cggaggtgct gacgccgctg atcaagctcg ccgccgaccg
       61 gggcgagctg cgcgccgacg ccgaccacgc ccagctcctc gcctacctgc tgctgttgct
      121 gccgcacctg gccctggcgc ccttcggccc ggagctcgac ccggccctgg gcctgtacgg
      181 caaggacccc gagcagctga ccgcccccgt ccacggcctg gtcggcgcgc tggagcgcgc
      241 cttcggccct gtcaaccacg tcccggacca gcagcgcccg gaaaggaccg cgcgatgacc
      301 gcgatcgacc gcaccgacac ctactgcgtc atcggcgccg gcagctccgg gctgaccgcc
      361 gccaagaacc tgcgcgagca cggcttcgcc gtggacgtct acgagcgcga ggacgacgtg
      421 ggcggcaact ggaacatcgg cgcggagaac agccgcgtgt accactccac ccacctgatc
      481 tcgtccaagc cgttcaccga gtaccccgac ttccccatgc ccgacgagtt ccccgactac
      541 ccgcaccaca gccagatgca cgcctacttc cgcgcctaca tgcgccactt cggcctcgac
      601 gaggtcatcc gcttctccac cgaagtggtc tcgtgcgagc cggtggacgg cgggcggcac
      661 tgggacgtga ccgtgcggcc gcgcggcagc aagggcaccc gcaagcagga ggtgttccgc
      721 tacgccgggc tggtgatcgc caacggccac aactggttcc cgaagatccc ggactacccc
      781 ggcgagttca ccggcgagct catgcactcg gcggcgtaca aggaccccga ggtgctgcgc
      841 ggcaagcgcg tgctggtcgt cggcgcgggc aacaccggct gcgacatcgc cgtggaggcc
      901 gcgcagaacg ccgcccgcac cctgcactcc acccggcgcg gctactggta ctcgcccaag
      961 tacagctacg gcaagcccag cgaccaggtc tacgacctca tgctgtcgct gaagctgccc
     1021 aagccgctgc tgcaacggat gatggagcgc acgctgaaga tgtcgcaggg cgacatcacg
     1081 cgcgtcggcc tgcgcgagcc cgaccaccac atcctggaga cgcaccccat cgtcaacagc
     1141 cagctcgtct actacgtggg ccacggcgag atcgatccca agcccgacat cgagcgcctg
     1201 gacggcgacg gggtggtgtt caccgacggc acgcgcgagg aggtcgacct catcgtgtgg
     1261 tgcaccggct acctggtgcg cttcccgttc atcgaccacg agcatctcaa ctgggcgggc
     1321 gaccacccga agctcttcca gaacgtgttc catccccgct acgacaacct gttcgtgatc
     1381 gggctgatcc agcccgacag cggccagttc aagctggtcc actggcagtc cgtcgccgtg
     1441 gcgcgctacc tggagtccca gcgcgcgcaa ccggcggccg ccgaggcgtt ccgccgccac
     1501 cgcgacgccc acttcgacga cccgctgtcc ggcggcgtgc actacaagga ctccacgcgc
     1561 cactacttcg agatcaacca ctacagctac ctggagggca tccaggagct gctgcacgtc
     1621 atggaggccg agcggccgtc caagagcaag gtcatggagc cgatgcgctg ggcgttcccg
     1681 tcctcgcccg tggcgcgcga ggtggtgacg cgcgagccgt cccagccggt ggacggacgg
     1741 ccgccgctgc tgttcgtgca cggcgccggc cacggcgcgt ggacgtggcg caactggatg
     1801 gcggcggccg ccgagcgcgg ctgggcctcc tgggcgctga gcctgcgcgg ccacggcgac
     1861 agcggcggcc acgaggacat caggcgctgg cgcctgcgcg actacgagca cgacgtgatg
     1921 cagacgatcg cgtcgatgcc gcagcccccg gtgctggtgg gccactccat gggcggcctg
     1981 gtggtccagc gcgtgctggc ccgctacccg gcgcgcgccg gcgtgctggt ggcggcggtg
     2041 ccgccgcgcc acggcatcga gacgacgctg gcgatcgcgc ggcgccaccc gcaggacctg
     2101 ctgaaggggc tggccggcgc ctcgctgccg tggcgcaagg agtacctgtt cacccccgac
     2161 acacccgacg aggtggcgct gccggtcatg cacgcgtgca cggacgagtc accgcgcgcg
     2221 cagatcgaga cgaccggccc gcacggcgcc tacccgtcca aggcgccggt gctggtgctg
     2281 ggcgcggagc gcgacggcct ggtgccgccg tcggccgtca tgcgcacggc ccgccactac
     2341 ggcaccaagg cgcacctgta ccgcggcatg ggccacgaca tgatgctgga cccgcactgg
     2401 cgcgcgcccc tggacctgat gctcgactgg ctggagaaga cgctcagcgc ctgacgccgg
     2461 tcagccggtg gcgaagtagc gcactttggc ggccatctcg gcggagaacg ccatgatcac
     2521 cgccaggtag aacatgccgg tcgccgcctg gatggagcgc ccgccctcct tggccagctt
     2581 caccacgaag ccgctgatga gcgcgcacac cgcgaccagg ccgccggcca gcagcacgct
     2641 gaagcccggc accgagaagt acagcgacag cccgcccgcc tccggcgagg ggttggccgc
     2701 cgacagcgcc gccgtgccga cgccggcgac cacgccggcg acgtaccacc aggcgctgcg
     2761 caccatcggg tccttggaca gcttgcgctc gaccaggtac cagtggccga gcagcagccc
     2821 gtagttggtg gcgcccagga acaccgcgcc cagcgccagc tcgagcaccg ccagcgccgg
     2881 gtcgtgcggg cgtgccagcc cgaacagcac cagggcgacc acgcccgccg ccgtggccgc
     2941 aaggccaacc gcccgcgaca ggccctggtg gacgaacagc agcacctgcc aggccagcgc
     3001 caaccccgcg aacacggcca gcgccaggac ggcgcggtcg gtggcgggcg acggcgccgc
     3061 cgtgaactcg ccgtcggcca ggcgccacgc gccgatcgcg ctcagcacga tcgtggagcc
     3121 gatgagcagg aagaagccgc gccgcaccct gccccacacg cccccggccc acagcaccac
     3181 ggcactgccg acagcggcct ccagcaggat gaccgacatg acgccgagcg gactggtcac
     3241 ggcaacctca cgcagaactt gggcgaacac cggatgtggg gttcatactg ccagcgcacc
     3301 gccgaggcac gaaaacgccc gcggcgcgcc ggaccaccac cgagggaagc tcatgagcgc
     3361 acacgacgac gaaggctgcc tgcccggccg ccgctgccgc atcacgctgc tcgtcggcga
     3421 ggccgacacg gccatcgcga tgggctccgg cgacgtcccc gtgctggcga gcccgcgcgt
     3481 cgtcgcgctg gcggagcagg cctccgtcga ggcgatcgcc ggcacgctgg acgagggcaa
     3541 gaccagcgtc ggcgcgtggg tggagctgga gcacaacgcc cccagccgcg tgggcgagac
     3601 ggtgaccgcc gaggcgacgc tgctcggcgt gcacgggcgc aagctggagt tctccgtcag
     3661 cgtgcgcaac gaggcgggcg aggaggtggc gcacgtgcgc caccgccgcg tcgtcgtcac
     3721 gcgcgccaag ttcgccggct gacgccgcgg agcggcgccg gccggctcag ccgcggctct
     3781 gcaggaagct gagcagggtg cggacgccga agccggtgcc ccccttggcg tt
//
LOCUS       DATMKC010000174        11706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_19048, whole genome shotgun sequence.
ACCESSION   DATMKC010000174 DATMKC010000000
VERSION     DATMKC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19048"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VNU01_05100"
     CDS             complement(<1..1002)
                     /locus_tag="VNU01_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWH32029.1"
                     /translation="MNLLAQATPVATPDIAWGAMTPELVLLGAAVLALMLDTAGRQRL
                     QTAFVVLGVLVAGAAFAWFQLDTVTGPALVALAGVAQFALTALWRDRPRLLGAIIAGL
                     GFSGALATTVWQWALHAGALRTAEGLELVGTETVLGGMFAVDGVALFTRLTVCLAGLV
                     TVPLGFAYAEERRMHRGEYYPLLLLAALGMTLLASSADLLMVFISVEILSLALYILCA
                     FAKRDLNAQESALKYFILGAFSSALLLYGIALVYGATGTTNIAEAGRAMSAIDANTAL
                     NIVAMGLLLVGFAFKTAVVPFHMWTPDVYQGAPTPITGFMAAATKGAAFAAFLRIFVG
                     "
     gene            complement(999..2558)
                     /locus_tag="VNU01_05105"
     CDS             complement(999..2558)
                     /locus_tag="VNU01_05105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWH32030.1"
                     /translation="MGSLGLDRVMSLMENLPVLTLLIAAPALTGLAIAFLDPERREQI
                     RAVAMGGALVTFALSLVLLARFQAGEAGFQLEETAQWIPQWGVTYRLGVDGVSLLLVA
                     LTAFVQPLVILATWNTELRAKGFFSSLMILQAALVGVFLALDLVLFYVMFEVMLVPMY
                     ALIGIWGSGNRHYAALKFFLYTLVGGLLMLVGILYLYFAAGATSFDYDVIRQVELSGT
                     EQFWLFLAFFAAFAIKVPMWPLHTWLPDAHTEAPTAGSVILAAVMLKIGGYGFLRFSL
                     PVFPDATERLAVPMLVLAVVGVLYGALVSIVQKDIKRLVAYSSVSHLGFVVLGVFALH
                     STAGAASVVQMVNHGLSTGALFLLIGFLYERAHTREIARYSGLLKSAPIFGGLFLVTA
                     MSSIALPGLNGFVGEFPILLGTFQTNPWAAVLSTFGVILAAIYLLWAYQRMFHGPVEG
                     KAVDFPDLNGREIAVMVPLVALMIGIGLYPKPLYDRVTPTVEQILAELQADDVIAGQR
                     PPQQHQEEPAS"
     gene            complement(2531..4456)
                     /gene="nuoL"
                     /locus_tag="VNU01_05110"
     CDS             complement(2531..4456)
                     /gene="nuoL"
                     /locus_tag="VNU01_05110"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWH32031.1"
                     /translation="MEHALTPPAGSVIQLAWLIPVIPALSAGFLLLAGKRLGKIAAPV
                     AIAAIGASFAVSLLVFGALRAEPEHERTFVVHLAQWLDIGPLQVDWNLLVDPLASVML
                     LLVTGVGTLIHVYSVGYMHGDERYPRFFAYLNLFAASMLILVLGESMLTLFVGWELVG
                     LCSYLLIGFWFERRDYAGAAKKAFVTNRVGDVGFMLAMFLAFSAVGSLSFTQVLPAAE
                     GLAAGTAVAIALLLFVGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYLV
                     ARMSPLFSAVPDVGMVVAWVGALTALLAALIACVQRDLKKILAYSTVSQLGYMFIAVG
                     VGAYTAGVFHLLTHGFFKALLFLAAGSVMHALHDETDVWRMGGLRKHMPVTFATSLIG
                     WLAIAGIPPLSGYFSKEEILAAASETPGAMPVYVIGLVVAGLTAFYMTRWFVLIFLGP
                     ERFGHNIHPHESPWSMSGVLAVLAVLAAGGGLLNTSAEGGWLHHWLEGSVVAFHGEVH
                     PLGHTALIALAMVAAVAGIAAAVALYRRVDAQGAPRFPGWHRLATNKFYVDELYQLVL
                     VTPGRMLSDALSVFDRRGIDGAVNGIARGTAGLATLGRRTQTGYVRSYALATLLGALL
                     IAGLFYAGSWAASAWIG"
     gene            complement(4467..4772)
                     /gene="nuoK"
                     /locus_tag="VNU01_05115"
     CDS             complement(4467..4772)
                     /gene="nuoK"
                     /locus_tag="VNU01_05115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWH32032.1"
                     /translation="MKVPAGHYLILAAALFTTGLVGVLVRRNLIVMFMCVELMLNSVN
                     LTLVAFSRIHGGLDGQIMSFFVMVVAAAEVTVGLALIINLFRLRASVDADDVHSLRS"
     gene            complement(4769..5464)
                     /locus_tag="VNU01_05120"
     CDS             complement(4769..5464)
                     /locus_tag="VNU01_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWH32033.1"
                     /translation="MAEFVVFWIMAPLALGAAISMLFMRNAVHAALLLVVNFFAIAVL
                     YAVLEAQFLAVIQIIVYAGAIMVLFLFVLMLLGINREEAGGGALPRQAPVAIALGVAL
                     FAVLAAGVASPFLSAEDACPTETPAEGQPVAAPQSDGGCAGLAAVNEEGNVRAVGLQL
                     FTRYVWPFEVVSALLIVAAVGAMVIGRKHEAPEDLVDQPANAMPEPRRDGPGPAPGES
                     HGDRADTERITKP"
     gene            complement(5466..6080)
                     /gene="nuoI"
                     /locus_tag="VNU01_05125"
     CDS             complement(5466..6080)
                     /gene="nuoI"
                     /locus_tag="VNU01_05125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWH32034.1"
                     /translation="MFKAMRKGFGLTFKTMFKPVVTTEYPEVKRPPQPRFHGRHVLNR
                     HPDGLEKCVGCELCAWACPADAIYVEGADNQPDAWFSPAERYGAVYQINYLRCILCGL
                     CIEACPTRALTMTSAYELSADSREALIYTKDQLLAPLPQGAKDTPHSAAEIAARNLDY
                     YQGPWAQYPLAHFRGLQGTAEELPQHDPAVAPRGTVSEPVHGEG"
     gene            complement(6081..7286)
                     /gene="nuoH"
                     /locus_tag="VNU01_05130"
     CDS             complement(6081..7286)
                     /gene="nuoH"
                     /locus_tag="VNU01_05130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWH32035.1"
                     /translation="MDGVDVLIIVLKAVAAFALWLTGTALMIWAERRVVARMQSRPGP
                     NRLGPFGLLQTLADGAKLFFKEDITPSNVDRVVYVAAPVMSAIVAMLTFAVIPFGGTF
                     EVAGREVALQVWDPDIGLLWFLAMSSIGVYGIVLAGWSSGSKYPLLGSVRSTAQMISY
                     ELAMGLSMAAVFIYTGSLRASEIVANQQGALLGFIPAWHVIPMAPAFVIFFVAVVAET
                     QRPPFDLPEAEGELVAGFATEYSAAKFAMFYLAEFMNVITVSAMTVTLFLGGPSGPAF
                     GPDWVQALMGLGYFLLKVAVLLFVFIWLRGTLPRIRYDRLMDLGWKVMLPLGLLWAMA
                     TSFSVVLRQTADQDTVLRLAGVGIAVAVALYLAAPLFGRGERDLAPAVPDPDWDDQAP
                     HDDRDGVNA"
     gene            complement(7286..9775)
                     /locus_tag="VNU01_05135"
     CDS             complement(7286..9775)
                     /locus_tag="VNU01_05135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HWH32036.1"
                     /translation="MVTLTIDGKEITVPKGTLVIRAAEQLGITIPRFCDHPLLDPVGA
                     CRQCMVEVEGQRKPFTSCTTTCTEGMVVRTHLTSEYAADAQEAQLEFLLINHPLDCPQ
                     CDKGGECPLQDQALAHGPSESRMVDEKRRYVKPVPISAQIALDRERCVLCARCTRFSQ
                     QIAGDPFIELFERGALEQVAIFADEPYHSYFSGNVAQICPVGALTATTYRFHARPFDL
                     QTHPGVCNQCSAGCNLRIDVRRGEVQRQLARDNMAVNESWNCDKGRFGFEYVGHPDRL
                     REPLVRDGLGQLQPAPWRYALQRAADGLRAAGEKVGVVTGARITDEDAYALSRFARGV
                     LGTDNVDFRLAPRSADEQAVLAAVSGTEGPTYDDIERAKVIVLAGLDAEEEVPILHLR
                     IRKAWRNHGARVVVVGPVLGSGHDYAARWVRTPAGDEAQVLRALAGDAGGDLPGDWAD
                     VADLLSGGGVVVLAGERLSRSPGALPAAAALAKARGGAFAWVPRRNNARGALDAGLMP
                     GVLPGGRSVAEPGPVAEAWGTVPAAVGLDTREMLEAAARGELKALYLVGVDLVRDFED
                     PELALAALGQVDTVIVQDLLPNDTTRLADVILPATDSHERVGSFTNWEGRRQPFRQAV
                     LPQGLAQEDWDIVRQLATVLGADLGWETPNDVRREAAPLMAAQGGALDRLPADLGARG
                     TDSLTPSANDPADGASPLTDAHPLRAIALDYLIGEQTMLLGAGALKATSRIPSVWLNE
                     TDARRAGIGQDDQVRVTSAHGSAVLPADVRDTVAAGTVVLPGLGTLPAIADVLAGAPA
                     GPFVAVGVERVGGPMPVVRDWVVGQAREGDA"
     gene            complement(9835..11175)
                     /gene="nuoF"
                     /locus_tag="VNU01_05140"
     CDS             complement(9835..11175)
                     /gene="nuoF"
                     /locus_tag="VNU01_05140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWH32037.1"
                     /translation="MAEPVKVLSQRYELEDAHTLDGYVGSGGYAALRTALGMEPAAIE
                     AMVKDSGLRGRGGAGFPTGVKWSFVPRDTGKPIYLVVNADEGEPGTFKDRELMERDPH
                     QLLEGMVIAGRALGSHRGFIYIRGEYLFAGMRLEQAVAEATARGYLGRDILGSGFDFE
                     ITVHFGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYACPTVVNNVETIANVP
                     HIVEHGVEWYRSMGTEKSPGPKLFCVSGEVVRPGNYEWPMGATAREIVEVSCQGMLPG
                     RSLKFWAPGGSSTPLLTAEHYDVPMDFESVAAAGSLLGTCAMMMFSDRTSVVEACLNW
                     TRFYEHESCGKCTPCREGVFWMAQILQRIVDGRGRTEDIDLLDSACEQIFGRTFCALG
                     DGATSPIVSAIRYFRDEFTHLIEHGRLPDHIEPHAGVVASNSGTDRPSMSGATA"
     gene            complement(11168..11671)
                     /locus_tag="VNU01_05145"
     CDS             complement(11168..11671)
                     /locus_tag="VNU01_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWH32038.1"
                     /translation="MATFYTMYKRERMGEHLVSVCTNFSCAVRGAKDVYQRLSDKLGV
                     GHNGTTADGKFTLEHAECLGNCEGAPVVTVDYFNYECLTPEQAEELIDRIAAGEIPPP
                     TRGPVPPGVRAAEYRLAGLGAFPQGGGGGGVVAAAAPSGQVPATDPEREAAADAGDRP
                     VGEHDNG"
BASE COUNT         1849 a         4218 c         4138 g         1501 t
ORIGIN      
        1 gcccacgaag atgcgcagga acgccgcgaa cgccgcaccc ttggtcgccg cggccatgaa
       61 gccggtgatc ggcgtgggcg cgccctggta gacgtccggg gtccacatgt ggaacggcac
      121 caccgcggtc ttgaacgcga agccgaccag cagcaggccc atggcgacga tgttcagcgc
      181 ggtgttcgcg tcgatcgcgc tcatcgcccg gccggcctcg gcgatgttcg tggtccccgt
      241 cgcgccgtag acgagcgcga tgccgtacag cagcagcgcg ctggagaacg cgcccaggat
      301 gaagtacttg agcgccgact cctgcgcgtt gaggtcgcgc ttggcgaacg cgcacaggat
      361 gtacagcgcc agcgacagga tctccacgga gatgaacacc atgagcaggt ccgcgctgga
      421 cgccagcaac gtcatgccga gcgccgccag cagcaggagc gggtagtact cgccgcggtg
      481 catgcggcgc tcctcggcgt aggcgaaacc gaggggcacg gtcaccaggc ccgccaggca
      541 caccgtcagg cgcgtgaaca gcgccacgcc gtcgacggcg aacatcccgc ccagcacggt
      601 ctccgtgccg accagctcca gcccttccgc cgtgcgcagc gcgccggcgt gcagggccca
      661 ctgccacacg gtcgtggcca gcgccccgct gaagccgaga ccggcgatga tcgcgccgag
      721 cagcctgggg cgatcccgcc acagcgcggt gagcgcgaac tgcgcgacgc cggcaagcgc
      781 gaccagcgcc ggcccggtga cggtgtcaag ctggaaccag gcgaacgcgg ctcccgccac
      841 cagcacgccg agcaccacga aggccgtctg cagccgctgg cgcccggcgg tgtccagcat
      901 gagcgcgagc accgcggcgc cgagcagcac cagctcgggc gtcatggcgc cccaggcgat
      961 gtcgggggtg gccaccggcg tggcctgagc gagcaggttc acgatgcggg ctcctcctgg
     1021 tgctgctggg gcggccgttg cccggcgatc acgtcgtcag cttgcagctc ggccaggatc
     1081 tgctccaccg tcggggtgac acggtcgtac agcggcttgg ggtacagccc gatgccgatc
     1141 atcagcgcga cgagcggcac catcacggcg atctcgcgcc cgttcaggtc cgggaagtcc
     1201 accgccttgc cctccaccgg gccgtggaac atgcgctggt aggcccacag caaatagatc
     1261 gcggccagga tcacaccgaa cgtggacagc accgccgccc acgggttggt ctggaacgtg
     1321 cccagcagga tcgggaactc gccgacgaag ccgttcaggc cgggcagcgc gatcgagctc
     1381 atggccgtga ccaggaacag cccgccgaag atcggcgcgg acttcagcag cccggagtac
     1441 cgcgcgatct cgcgcgtgtg ggcgcgctcg tacaggaagc cgatcagcag gaacagcgcg
     1501 ccggtggaca ggccgtggtt gaccatctgc accacggacg ccgcgccggc ggtggagtgc
     1561 agcgcgaaca cgcccagcac cacgaagccg aggtggctga cggagctgta ggccaccagc
     1621 cgcttgatgt ctttctgcac gatcgacacc agcgcgccgt acagcacgcc caccacggcc
     1681 agcacgagca tcggcacggc gaggcgctcg gtggcgtccg ggaacaccgg cagggagaag
     1741 cgcaggaagc cgtagccgcc gatcttcagc atgacggccg ccaggatcac cgagccggcc
     1801 gtgggcgcct cggtgtgcgc gtcgggcagc caggtgtgca gcggccacat cggcaccttg
     1861 atcgcgaagg cggcgaagaa ggcgaggaac agccagaact gctcggtccc cgacagctcc
     1921 acctgccgga tcacgtcata gtcgaacgac gtcgccccgg cggcgaagta caggtacagg
     1981 atgcccacca gcatcagcag gccgccgacc agcgtgtaca ggaagaactt cagcgccgcg
     2041 tagtggcggt tgcccgagcc ccagatgccg atgagggcgt acatcggcac gagcatgacc
     2101 tcgaacatca cgtagaacag caccaggtcc agcgccagga acacgcccac cagcgcggcc
     2161 tgcaggatca tcagcgagct gaagaagccc ttggcgcgca gctcggtgtt ccacgtcgcc
     2221 aggatcacca gcggctggac gaacgcggtc agcgccacca gcagcaggct cacgccgtcc
     2281 acgccgagcc ggtaggtgac gccccactgc ggaatccact gggcggtctc ctccagctgg
     2341 aagccggcct cgcccgcctg gaagcgggcg agcagcacca gcgacagcgc gaaggtgacg
     2401 agcgccccgc ccatcgccac ggcacggatc tgctcgcggc gctccgggtc caggaacgcg
     2461 atcgccaggc cggtgagcgc cggggcggcg atcagcaggg tcagcacggg caggttctcc
     2521 atcagcgaca tcacccgatc caggccgagg ctgcccacga gcccgcgtag aagagcccgg
     2581 cgatcagcag cgcccccagc agcgtggcga gcgcgtagct gcgcacgtag ccggtctgcg
     2641 tgcgccgccc cagcgtggcc aggccggcgg tgccgcgcgc gatgccgttc acggcgccgt
     2701 cgatgccgcg gcggtcgaac acgctcagcg cgtccgacag catccggcca ggcgtcacca
     2761 gcaccagctg gtacagctcg tcgacgtaga acttgttggt ggcgaggcgg tgccagccgg
     2821 ggaagcgcgg cgcgccttgc gcatcgacac ggcggtacag cgccacggcg gcggcgatgc
     2881 cggccacggc cgcgaccatc gccagcgcga tgagcgccgt gtggccgagc gggtggacct
     2941 cgccgtggaa ggccacgacg gagccctcca gccagtggtg cagccagccg ccctcggcgg
     3001 aggtgttgag gaggccgccg cccgcggcga gcacggccag caccgccagc acgccgctca
     3061 tgctccacgg cgactcgtgc gggtggatgt tgtgtccgaa gcgctccggc cccaggaaga
     3121 tcagcacgaa ccagcgcgtc atgtagaagg cggtgagacc cgccaccacc aggccgatga
     3181 cgtacaccgg catcgcgccc ggcgtctccg aggccgcggc caggatctcc tccttggaga
     3241 agtagcccga cagcggcggg atgcccgcga tcgccagcca gccgatgagc gacgtggcga
     3301 acgtgaccgg catgtgcttg cgcaggccgc ccatgcgcca cacgtcggtc tcgtcgtgca
     3361 gcgcgtgcat caccgagccg gcggccagga acagcagcgc cttgaagaac ccgtgcgtca
     3421 ggaggtggaa gaccccggcg gtgtaggcgc ccacgcccac cgcgatgaac atgtaaccga
     3481 gctggctcac cgtggagtac gccaggatct tcttgaggtc gcgctgcacg caggcgatca
     3541 gggccgcgag cagcgccgtg agcgcaccca cccaggcgac caccatgccg acgtccggca
     3601 ccgcgctgaa cagcggcgac atgcgcgcca ccaggtacac ccctgcggtc accatggtgg
     3661 cggcgtggat gagtgccgac accggcgtgg ggccggccat cgcgtccggc agccagacgt
     3721 acagcgggat ctgcgcgctc ttgccggtcg cgcccacgaa cagcagcagc gcgatcgcca
     3781 cggccgtgcc cgccgccagg ccctcggcgg ccggcagcac ctgcgtgaac gacagggagc
     3841 ccaccgcgga gaaggcgagg aacatcgcca gcatgaagcc gacgtcgccc acgcggttgg
     3901 tgacgaaggc cttcttggcc gcgccggcgt agtcgcgccg ctcgaaccag aagccgatca
     3961 acaggtagga gcacaggccc accagctccc agcccacgaa cagcgtgagc atgctctcgc
     4021 cgagcacaag gatcagcatc gaggcggcga acaggttgag gtaggcgaag aagcgggggt
     4081 agcgctcgtc cccgtgcatg tagccgaccg agtagacgtg gatcagcgtg cccacgccgg
     4141 tgaccagcag cagcatcacg gacgccagcg ggtccaccag caggttccag tcgacctgca
     4201 gcgggccgat gtccaaccac tgcgccaggt gcaccacgaa ggtgcgctcg tgctccggct
     4261 cggcgcgcag cgcgccgaac accagcagcg acacggcgaa gctcgcgccg atggcggcga
     4321 tggccacggg cgcggcgatc ttgcccagcc gcttgccggc cagcagcagg aagcccgcgc
     4381 tgagcgcggg gatcaccggg atgagccagg cgagctggat cacggagccg gcgggtgggg
     4441 tgagcgcgtg ttccacggat tgctccctag gaccgcagcg agtggacgtc gtcggcgtcc
     4501 accgacgcgc gcaggcggaa gaggttgatg atcagcgcca ggcccaccgt gacctcggcg
     4561 gcggcgacga ccatgacgaa gaacgacatg atctggccgt cgaggccgcc gtggatgcgc
     4621 gagaacgcca ccagcgtcag gttcacgctg ttgagcatca gctccacgca catgaacatc
     4681 acgatcaggt tgcggcgcac gagcacgccg acgagcccgg tcgtgaacag cgccgcggcg
     4741 aggatcaggt agtggccggc ggggaccttc atggcttcgt gatcctttcc gtgtcggccc
     4801 tgtccccgtg cgactcccca ggcgctgggc cggggccgtc gcggcgcggc tccggcatcg
     4861 cgttggcggg ctggtcgacc aggtcctccg gcgcctcgtg cttgcgcccg atcaccatgg
     4921 cgccgacggc cgccacgatg agcagcgcgc tgacgacctc gaacggccac acgtagcggg
     4981 tgaacagctg caggcccacc gcgcgcacgt tgccctcctc gttgaccgcc gccagccccg
     5041 cgcagccgcc gtcgctctgg ggagcggcga cgggctggcc ctccgccggc gtttccgtgg
     5101 ggcaggcgtc ctcggcggac aggaacggcg aggcgacacc ggccgcgagc accgcgaaca
     5161 gggccacgcc cagcgcgatc gccaccggcg cctggcgcgg cagcgctccc ccgccggcct
     5221 cctcgcgatt gatgcccagc agcatgagca cgaacaggaa caggaccatg atcgcgccgg
     5281 cgtagacgat gatctggatc accgccagga actgcgcctc cagcaccgcg tacagcaccg
     5341 cgatggcgaa gaagttcacc accagcagca gcgcggcgtg gaccgcgttg cgcatgaaca
     5401 gcatggagat ggcggcgccc agcgcgagcg gcgccatgat ccagaagacg acgaactcgg
     5461 ccatctcagc cctctccgtg gaccggctcg gacacggtgc cgcgcggcgc cacggcggga
     5521 tcgtgctgcg gcagctcctc cgcggtgccc tgcagccccc ggaagtgcgc gagcgggtac
     5581 tgcgcccatg ggccctggta gtagtcgagg ttgcgggccg cgatctcggc cgcgctgtgc
     5641 ggcgtgtcct tggcgccttg cggcagcggg gccaacagct ggtccttggt gtagatcagc
     5701 gcctcgcggc tgtccgcgga cagctcgtag gcgctggtca tcgtgagcgc gcgggtgggg
     5761 cacgcctcga tgcacaggcc gcagaggatg cagcgcaggt agttgatctg gtacacggcg
     5821 ccgtagcgct ccgcgggcga gaaccacgcg tcgggctggt tgtcggcgcc ctccacgtag
     5881 atggcgtcgg ccgggcaggc ccaggcgcac agctcgcagc cgacgcactt ctccaggccg
     5941 tcgggatggc ggttgagcac gtggcgcccg tggaagcgcg gctgcggcgg ccgcttcacc
     6001 tccgggtact ccgtggtgac caccggcttg aacatggtct tgaacgtcag gccgaagccc
     6061 ttgcgcatgg ccttgaacat ctacgcgttc accccgtccc tgtcgtcatg gggggcttgg
     6121 tcgtcccagt cggggtccgg cacggcgggc gcgaggtcgc gctcgcccct gccgaacagc
     6181 ggcgccgcca ggtacagcgc cacggccacc gcgatgccga cgcccgccag ccgcagcacg
     6241 gtgtcctggt cggcggtctg gcgcagcacc acggagaagg acgtcgccat cgcccacagc
     6301 aggcccagcg gcagcatgac cttccagccc aggtccatca ggcggtcgta gcggatgcgc
     6361 ggcagcgtgc cgcggagcca gatgaacacg aacagcagga cggcgacctt gagcaggaag
     6421 taccccagtc ccatgagcgc ctggacccag tccggtccga acgccgggcc gctcggcccg
     6481 cccaggaaca gcgtcacggt catggccgac accgtgatca cgttcatgaa ctcggccagg
     6541 tagaacatcg cgaacttggc ggcggagtac tccgtggcga agccggcgac cagctcgccc
     6601 tccgcctcgg gcaggtcgaa cggcggccgc tgggtctccg ccaccacggc cacgaagaag
     6661 atcacgaacg ccggcgccat cgggatgacg tgccaggcgg ggatgaagcc gagcagtgcg
     6721 ccctgctggt tcgccacgat ctcgctggcc cgcagcgagc cggtgtagat gaacaccgcc
     6781 gccatggaca gccccatcgc caactcgtag gagatcatct gcgcggtgga ccgcacgctg
     6841 cccagcagcg ggtacttgga gccggacgac cagccggcga gcacgatgcc gtagacgccg
     6901 atggaggaca tcgccaggaa ccacaagagg ccaatgtcgg ggtcccacac ctgcagggcc
     6961 acctcgcgcc cggcgacctc gaaggtgccg ccgaagggga tcaccgcgaa ggtgagcatg
     7021 gccacgatcg ccgacatcac cggcgccgcc acgtacacca cgcggtcgac gttggacggc
     7081 gtgatgtcct ccttgaagaa cagcttggcg ccgtccgcga gcgtctggag caggccgaac
     7141 gggcctagcc ggttggggcc cgggcgcgac tgcatgcgcg cgaccacgcg ccgctccgcc
     7201 cagatcatca gggcggtgcc ggtgagccag agcgcgaagg cggcgaccgc cttgagcacg
     7261 atgatgagca cgtccacgcc gtccatcagg cgtctccctc ccgcgcctgg ccgaccaccc
     7321 agtcacgcac gaccggcatc gggccgccca cgcgctcgac gcccacggcg acgaacgggc
     7381 cggcgggggc gccggccagc acgtcggcga tggccgggag ggtgcccagg cccggcagca
     7441 cgaccgtgcc cgcggcgacg gtgtcgcgca cgtcggccgg cagcacggcg ctgccgtgcg
     7501 cgctggtcac gcgcacctgg tcgtcctggc cgatgccggc gcggcgcgcg tcggtctcgt
     7561 tgagccacac cgacgggatc cggctggtgg ccttcaaggc gccggcaccg agcagcatgg
     7621 tctgctcgcc gatcaggtag tccagcgcga tcgcgcgcag cgggtgcgcg tcggtcagag
     7681 gcgatgcgcc gtccgccgga tcgttggcac tcggtgtgag cgagtccgtg ccgcgcgcac
     7741 ctaggtccgc cggcagccgg tccagcgcac cgccctgggc ggccatgagc ggcgccgcct
     7801 cgcggcgcac gtcgttgggc gtctcccagc ccaggtcggc gcccagcacc gtggcgagct
     7861 ggcgcacgat gtcccagtcc tcctgcgcca gcccctgcgg caggacggcc tggcggaacg
     7921 gctggcggcg gccctcccag ttcgtgaagg agcccacgcg ctcgtgggag tcggtggcgg
     7981 gcaggatcac gtcggccagt cgcgtggtgt cgttgggcag gaggtcctgg acgatcaccg
     8041 tgtcgacctg gccgagggcg gcgagcgcca gctcggggtc ctcgaagtcg cgcaccaggt
     8101 cgacgccgac caggtacagc gccttgagct cgccgcgcgc ggcggcctcc agcatttcgc
     8161 gggtgtccag cccgaccgcg gccggcaccg tgccccaggc ctcggcgacc gggcccggct
     8221 cggccacgct gcgcccgccc ggcagcacgc cgggcatcag gccggcgtcc agcgcgccgc
     8281 gcgcgttgtt gcgccgcggc acccacgcga acgcgccgcc gcgcgccttg gccagggcgg
     8341 ccgccgcggg cagggcgccc ggtgagcgcg acaggcgctc gccggccagc accaccacgc
     8401 cgccgccgct cagcaggtcc gccacgtcgg cccaatcacc aggcaggtct ccgccggcgt
     8461 caccggccag cgcgcgcagg acctgcgcct cgtccccggc gggcgtgcgc acccagcgcg
     8521 cggcgtagtc gtggccgctt cccagcaccg ggccgaccac gaccacccgc gccccgtggt
     8581 tgcgccacgc cttgcggatg cgcaggtgga ggatgggcac ctcctcctcc gcgtccagcc
     8641 cggcgagcac gatcaccttg gcgcgctcga tgtcgtcgta ggtcggcccc tccgtgccgc
     8701 tgacggccgc gagcaccgcc tgctcgtcgg cgctgcgcgg cgccaggcgg aagtcgacgt
     8761 tgtccgtgcc aagcacgccg cgggcgaagc gcgacagcgc gtaggcgtcc tcgtcggtga
     8821 tgcgggcgcc ggtgacgacg cccaccttct ccccggcggc gcgcaggccg tcggcggcgc
     8881 gctggagggc gtagcgccac ggcgccggct gcagctgccc cagcccgtcg cggaccagcg
     8941 gctcgcgcag gcggtcgggg tggcctacgt attcgaagcc gaagcggccc ttgtcgcagt
     9001 tccacgactc gttgaccgcc atgttgtcgc gcgcgagctg gcgctggacc tcgccgcggc
     9061 gcacgtcgat gcgcaggttg cagccggcgc tgcactggtt gcacacgccc ggatgggtct
     9121 gcaggtcgaa cggccgggcg tggaagcggt acgtcgtcgc ggtcagcgcg ccgaccgggc
     9181 agatctgcgc gacgttgccg gagaagtagg agtggtacgg ctcgtcggcg aagatggcca
     9241 cctgctccag ggcaccgcgc tcgaacagct cgatgaacgg gtcgccggcg atctgctggg
     9301 agaagcgcgt gcagcgggcg cacagcacgc agcgctcgcg gtccagcgcg atctgcgcgg
     9361 agatcggcac cggcttcaca taccggcgct tctcgtcgac catccggctc tcggacggcc
     9421 cgtgcgccag ggcctggtcc tgcagcgggc actcgccgcc cttgtcgcac tgcgggcagt
     9481 ccagcgggtg gttgatgagc aggaactcca gctgcgcctc ctgcgcgtcc gcggcgtact
     9541 cggacgtcag gtgcgtgcgg acgaccatgc cctcggtgca cgtggtggtg caggacgtga
     9601 acggcttgcg ctgcccctcg acctcgacca tgcactggcg gcacgccccc accgggtcca
     9661 gcagcggatg gtcgcagaag cgcgggatgg tgatgccgag ctgctcggcg gcgcggatga
     9721 ccagcgtgcc cttgggcacg gtgatctcct tgccgtcgat cgtgagggtg accacgtctg
     9781 ctggcaggcc actaccgccc tgcgggctgc ttgagtggct tcgcgcgatg tcggtcatgc
     9841 ggtcgctccc gacatggagg gccggtcggt gccgctgttg gacgcgacga cccccgcgtg
     9901 gggctcgatg tggtccggca ggcggccgtg ctcgatcagg tgcgtgaact cgtcgcggaa
     9961 gtacctgatg gccgacacga tcgggctcgt ggcgccgtcg cccagcgcgc agaaggtgcg
    10021 gccgaagatc tgctcgcagg cggagtccag caggtcgatg tcctccgtgc ggccgcgacc
    10081 gtcgacgatg cgctggagga tctgggccat ccagaacacg ccctcgcggc acggggtgca
    10141 cttgccacag gactcgtgct cgtagaagcg cgtccagttc aggcacgcct ccacgacgct
    10201 ggtcctgtcg ctgaacatca tcatggcgca ggtgcccagc agcgagccgg ccgccgccac
    10261 cgactcgaag tccatcggca cgtcgtagtg ctcggcggtc agcagcggcg tcgacgagcc
    10321 gcccggcgcc cagaacttca gcgagcggcc gggcagcatg ccctggcagc tcacctcgac
    10381 gatctcgcgg gcggtggcgc ccatcggcca ctcgtagttg ccggggcgca ccacctcgcc
    10441 cgacacgcag aacagcttgg ggccgggcga cttctcggtc cccatggagc ggtaccactc
    10501 cacgccgtgc tccacgatgt ggggcacgtt ggcgatggtc tcgacgttgt tgaccaccgt
    10561 ggggcaggcg tacaggccgg ccaccgccgg gaacggcggg cgcaggcgcg gctgcccgcg
    10621 ccggccctcc agggagtcca gcagcgcggt ctcctcgccg cagatgtagg cgccggcgcc
    10681 gaagtgcacc gtgatctcga agtcgaagcc cgagcccagg atgtcccggc ccaggtagcc
    10741 gcgcgcggtc gcctcggcca cggcctgctc caggcgcatg ccggcgaaca ggtactcgcc
    10801 gcggatgtag atgaagccgc ggtggctgcc cagggcgcgc ccggcgatca ccatgccctc
    10861 cagcagctgg tgcgggtcgc gctccatcag ctcgcggtcc ttgaacgtcc cgggctcgcc
    10921 ctcgtccgcg ttgaccacca ggtagatcgg cttgccggtg tcgcgcggca cgaagctcca
    10981 cttcacgccg gtggggaagc cggcgccgcc gcggccgcgc agccctgagt ccttcaccat
    11041 cgcctcgatg gcggccggct ccatgcccag cgcggtgcgc agggcggcgt agccgccgct
    11101 gcccacgtag ccatccaggg tgtgcgcgtc ctccagctcg tagcgctggc tcaggacctt
    11161 gaccggctca gccattgtcg tgctccccga ccgggcggtc gcccgcgtcc gcggcggcct
    11221 cgcgctccgg gtccgtcgcc ggcacctggc cgctgggcgc ggccgccgcc accacgccgc
    11281 caccgccgcc gccttgcggg aacgctccca ggccggccag ccggtactcg gcggcgcgca
    11341 cgcccggcgg gaccggcccg cgcgtgggcg gcgggatctc gcccgccgcg atccggtcga
    11401 tgagctcctc ggcctgctcg ggcgtgaggc actcgtagtt gaagtagtcc accgtgacga
    11461 ccggcgcgcc ctcgcagttg cccaggcact cggcgtgctc cagcgtgaac ttgccgtcgg
    11521 cagtggtgcc gttgtgaccc acgcccagct tgtctgacag ccgctggtag acgtccttcg
    11581 cgccgcgcac cgcgcaggag aagttcgtgc acacgctgac caggtgctcg cccatccgct
    11641 cgcgcttgta catggtgtag aaggtcgcca ccgccgcgac cttctacacc atgtacaagc
    11701 gcgagc
//
LOCUS       DATMKC010000175         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_78421, whole genome shotgun sequence.
ACCESSION   DATMKC010000175 DATMKC010000000
VERSION     DATMKC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78421"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VNU01_05150"
     CDS             complement(<1..527)
                     /locus_tag="VNU01_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWH32039.1"
                     /translation="MRAIQIEEFGGPEVLQIVQLPDPQPKPGQVLLDVARVGVNYADT
                     HQAENSYLSPTTLPMIPGAEVAGTTPDGRRVVAFVDGGAYAERAVAWEPLLFDVPDGV
                     DDGQALALLLQGLTAWHILRTSARIEPGESVVVHAAAGGVGSLAVQLAKQFGAGRVIG
                     AASTEDKRELVRELGA"
     gene            728..1783
                     /locus_tag="VNU01_05155"
     CDS             728..1783
                     /locus_tag="VNU01_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWH32040.1"
                     /translation="MADQAGRRILITGIASPLAARLAARLEADDRVEHLVGIDLREPA
                     RKLERTEYVRADLRNPLVAKILDSSRADTVVHLSITAAPHRAGGRARMKELNVIGTMQ
                     LLAAAQKSTRLRKLVLKSTTAVYGSNYADPALFREEVAPGGAPRSGYSKDAVEVEGYG
                     RAFGRRRSDVTLSVLRFANFIGPDVDSPLTRYLALPVVPMVLGYDPRLQLVHSDDAVE
                     ILYRSTMEEHPGIYNVAGPGIVYLSQAVRLAGKPSIPVPHPFVNGLAGMVRRSGVVDF
                     SPEQLQFLMFGRVGSIDRMRAEFGYTPAYSTKEALLDFLRSGRVTPLIDRERVERAER
                     DLGELIKRAGRMLAPQR"
     gene            1818..>2316
                     /locus_tag="VNU01_05160"
     CDS             1818..>2316
                     /locus_tag="VNU01_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32041.1"
                     /translation="MSRAGQAGAGAEVIDLDPVRRRGAARRDGAPRQRCAHRAGDVAC
                     RNYAGPTGYCRVHEPEDAAAPVARPETFGAPPPLDPEDFDEAVDKALAFLRRRLTGDY
                     EVDVFGFDRELTEQVLMPLARPLYQKYWRVRTVGLEHVPSDSGALVVANHAGTLPFDA
                     IMTKMA"
BASE COUNT          331 a          851 c          822 g          312 t
ORIGIN      
        1 gcccccagct cgcgcaccag ctcgcgcttg tcctcggtgg atgcggcgcc gatcacgcgc
       61 ccggcgccga actgcttggc gagctgcacg gccagcgagc cgaccccgcc ggcggccgcg
      121 tgcacgacca ccgactcgcc cggctcgatg cgcgccgagg tgcgcaggat gtgccaggcg
      181 gtgagccctt gcagcagcag cgccagtgcc tggccgtcgt ccacgccgtc cgggacgtcg
      241 aacagcagcg gctcccaggc cacggctcgt tccgcgtacg ccccgccgtc gacgaaggcg
      301 actacgcgac gcccgtccgg cgtggtgccg gccacctcgg cgccggggat catcggcagg
      361 gtggtggggg acaggtagct gttctcggcc tgatgcgtgt cggcgtagtt gacgccgacg
      421 cgcgcgacgt ccaacagcac ttgcccgggc ttgggctgcg ggtccggaag ctgcacgatc
      481 tgcagcacct cgggtccgcc gaactcttcg atctggatcg cgcgcatgtg ctcccgctct
      541 cgtcgcccac cggcttgcca ggcgggagca tagaggcgga caggccggat gcgccccctg
      601 ggccatcggg ccgggtaatc gggtggttgc gggtccttcc cggggcgtag gcttgcgccg
      661 acggcgcggg gccgcctcca cggcccatcc ccacgacgcg caccgaccag aaagcgacca
      721 gctggacgtg gcagaccaag ccggacggcg aatcctcatc accggcatcg cgagtcctct
      781 cgcggcgcgc ctcgccgcgc gcctggaggc agacgaccgg gtcgagcacc tggtcgggat
      841 cgacctgcgc gagccggcgc ggaagctcga gcgcaccgag tacgtgcgcg cggacctgcg
      901 caacccgctg gtcgcgaaga tcctcgactc cagccgcgcc gacacggtgg tgcacctgtc
      961 catcaccgcg gcgccgcacc gcgccggcgg ccgcgcgcgc atgaaggaac tcaacgtcat
     1021 cggcaccatg cagctgctgg cggcggcgca gaagtcgacg cggctgcgca agctggtgct
     1081 gaagtcgacc accgccgtgt acggcagcaa ctacgccgac cccgcgctgt tccgcgagga
     1141 ggtcgcgccg ggcggcgcgc cgcgctccgg ctactccaag gacgccgtgg aggtggaggg
     1201 ctacggccgc gcgttcggcc gccgccgcag cgacgtcacc ttgagcgtgc tgcggttcgc
     1261 caacttcatc ggtcccgacg tcgactcgcc gctgacgcgc tacctggcgc tgcccgtggt
     1321 cccgatggtg ctgggctacg acccgcgcct gcaactggtc cactccgacg acgccgtgga
     1381 gatcctgtac cgctccacca tggaggagca cccgggcatc tacaacgtcg ccgggccggg
     1441 gatcgtgtac ctgtcccagg ccgtgcgcct ggccggcaag ccgtccatcc cggtgccgca
     1501 cccgttcgtg aacggcctgg cgggcatggt gcgccgcagc ggggtggtcg acttctcgcc
     1561 cgagcagctg cagttcctca tgttcggccg cgtcggctcc atcgaccgca tgcgcgccga
     1621 gttcggctac acaccggcgt actccaccaa ggaagccctg ctggacttcc tgcggtcggg
     1681 cagggtcacg ccgctgatcg accgggaacg agtggagcgc gccgagcgcg atctcggcga
     1741 gctgatcaag cgcgccgggc gcatgctggc gccgcagcgt tgagcaggac cgacgacggt
     1801 tcgaggatgt gaccaggatg agcagggcag gacaagcggg agcaggcgcg gaggtcatcg
     1861 acctcgaccc ggtgcgacgg cgaggcgccg ccaggcgcga cggcgcgccc cggcagcgct
     1921 gcgcccaccg cgcgggcgac gtcgcctgcc gcaactacgc cgggccgacc ggttactgcc
     1981 gcgtccacga gcccgaggat gcggccgctc cggtggctcg acccgagacg ttcggcgcgc
     2041 cgccgccgct tgacccggag gacttcgacg aggcggtcga caaggccctt gccttcctgc
     2101 gccgccggct caccggcgac tacgaggtcg acgtcttcgg cttcgaccgc gagctcaccg
     2161 agcaggtgct gatgccgctc gcgcggccgc tgtatcagaa gtactggcgg gtgcgcacgg
     2221 tcgggctgga gcacgtgccc agcgacagcg gcgcgctggt cgtggccaac cacgccggca
     2281 cgctgccgtt cgacgcgatc atgaccaaga tggccc
//
LOCUS       DATMKC010000176         3173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_338158, whole genome shotgun sequence.
ACCESSION   DATMKC010000176 DATMKC010000000
VERSION     DATMKC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338158"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VNU01_05165"
     CDS             complement(<1..680)
                     /locus_tag="VNU01_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32042.1"
                     /translation="MTSPTPDVSAEPAAAPDEPKKQGKKKAPPPEPRDIVRRIGSDSE
                     AALAYVLAPDPQLSRLGRKVKEELITELPPVTAAALLGVEALARHFLASAAAGRYRDL
                     FFMWELFRERPDECRQVLAERPKAVEKGRHALQTAYRLGIKGHAERVAQDVARAQGLV
                     WQWLREEVHADLAAVGRRPGVAAALLRREPDTEVPLPDSPDADWLAEAVAARADGELP
                     AAIDALVAA"
     gene            complement(747..2006)
                     /locus_tag="VNU01_05170"
     CDS             complement(747..2006)
                     /locus_tag="VNU01_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWH32043.1"
                     /translation="MSAETPGAADKPPAPDRRRAPRAKVDLGGDLTDAVLQGLPDRHV
                     IAHPELFERRPRIGVVLGAGGVLGNAYLSGVMAALHAVTGFEPRTATALVGTSAGSVH
                     AALYGAGMPALFGLWRTRGGRLPEAFTFRPADADPGDDEHVDHGEDATLREIFTPARA
                     LPKLGPSSKSLAARALVRPWRYRPEVVLSAWLPEGVLTNAAIGRALRGVVPAGWVHHP
                     RTWITAVNLRTGEREVFGRPGSQRAHLDRAVRASCAIPGLYRPVRIGRERYVDGGIHS
                     PSNADVLTGMDLDLVLVVNPMSSLEGHAASGFVDRYLYPLRRMAGRRLDAELRALRED
                     GTPTLVLQPTARDLAVFSRNLMDPRPRSAVAERAVETTLDLLRDRAHQPALDLLRLAA
                     RSRRLRPAANAAARSAQAAAQYPRSRP"
     gene            complement(2003..2962)
                     /locus_tag="VNU01_05175"
     CDS             complement(2003..2962)
                     /locus_tag="VNU01_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH32044.1"
                     /translation="MRSERIRVERAALRRGPGAHDVHAAHWGAAGTGGALTVVCVHGL
                     GGSHLNWRLIGPLLAALPDVGTVWAPDLAGFGLTPLAAADGGERRATLADNGDLLRGF
                     LATVAPGGPVVLVGNSMGGLLSLGLAARHPELVRGIALVNPAVPAPLGARLDPQVVAN
                     FAAFALPRVGESVLARRQRRLSPRQQVQQTMALCVADPEALDAELLDAHTDLAARRRT
                     MPYAHRAFLQAARDVVRAVTVGRNRVWDAVARIGAPGLYLQGAQDRLIRPETGALLLR
                     HRPDWDHVRYEDLGHVPMIEDPDRVAGDLHRWLERRVSAATTP"
     gene            complement(2972..>3173)
                     /locus_tag="VNU01_05180"
     CDS             complement(2972..>3173)
                     /locus_tag="VNU01_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HWH32045.1"
                     /translation="PTDTDEEADPREGWTTYTVKSGDTLISIAQDVYGDGTKFNLIAD
                     ANGIDKNNKLRVGQELQIPPAD"
BASE COUNT          361 a         1197 c         1237 g          378 t
ORIGIN      
        1 gccgcgacca gcgcgtcgat ggcggcgggc agctcaccgt cggcgcgcgc ggccaccgcc
       61 tccgccagcc agtcggcgtc ggggctgtcc ggcagcggca cctcggtgtc cggctcgcgg
      121 cgcagcagcg cggcggccac cccgggccgg cggcccaccg cggccaggtc cgcgtggacc
      181 tcctcgcgca gccactgcca caccagcccc tgagcccggg cgacatcctg agcgacgcgc
      241 tcggcatggc ccttgatgcc caggcggtag gccgtctgca gggcgtggcg gcccttctcc
      301 acggccttgg gccgctcggc caggacctgc cggcattcgt cggggcgctc gcggaacaac
      361 tcccacatga agaacaggtc ccggtagcgc ccggcggcgg cggaggccag gaagtggcgc
      421 gccagcgcct ccacgcccag cagcgccgcc gcggtgaccg gtggcagctc ggtgatgagc
      481 tcctccttga ccttgcggcc caggcgcgaa agctgcgggt ccggcgccag cacgtaggcc
      541 agcgccgcct cgctgtccga gccgatgcgc cggacgatgt cgcgcggctc cggcggcggc
      601 gccttcttct tgccctgctt cttcggctcg tcgggagccg cggcgggctc ggcggacacg
      661 tcgggcgtgg gcgaggtcat ggcgggctgc cttctgccgg gcggtcggac tggtcccgcg
      721 ggcgggcgcg cgcgagggtc ggcgggtcag ggacgcgacc gaggatactg tgcggccgct
      781 tgcgcggagc gcgcggcggc gttcgccgcg gggcgcaggc gccgcgatcg cgcggccagc
      841 cgcagcaggt ccagggccgg ctggtgcgcc cggtcgcgca gcaggtccag cgtcgtctcc
      901 accgcgcgct ccgccacggc gctgcgcgga cgggggtcca tgaggttgcg cgagaacacc
      961 gcgaggtcgc gcgcggtcgg ctggagcacc agcgtcggcg tgccgtcctc gcgcagggcg
     1021 cgcagctcgg cgtccaggcg ccgcccggcc atgcgccgca gcgggtacag gtagcggtcc
     1081 acgaagccgc tggccgcgtg cccctccagg ctggacatgg ggttgaccac caggaccaaa
     1141 tccaggtcca tccccgtgag cacgtcggcg ttgctggggg agtggatgcc gccgtcgacg
     1201 tagcgctccc gcccgatgcg caccgggcgg tacaggcccg ggatggcgca cgacgcgcgc
     1261 accgcgcggt ccaggtgcgc gcgctgggac ccgggccggc cgaacacctc gcgctcgccg
     1321 gtgcgcaggt tcacggcggt gatccacgtc cgcgggtggt gcacccagcc ggccggcacc
     1381 acgccgcgca gcgcgcggcc gatggcggcg ttggtcagca cgccctcggg cagccacgcg
     1441 gacagcacca cctccggccg gtagcgccac gggcgcacca gcgcccgcgc cgccaaggac
     1501 ttcgaggacg gtcccagctt cggcagcgcg cgcgccggcg tgaagatctc gcgcagggtg
     1561 gcgtcctcgc cgtggtccac gtgctcgtcg tcgccggggt ccgcgtccgc cgggcggaac
     1621 gtgaacgcct ccggcaggcg cccgccccgc gtgcgccaca ggccgaacag cgccggcatc
     1681 ccggcgccgt acagcgccgc gtggacggac ccggcgctcg tgcccaccag cgccgtggcc
     1741 gtgcgcggct cgaagccggt gacggcgtgc agcgccgcca tcacccccga caggtaggcg
     1801 ttgcccagga cgccgccggc gcccagcacc acgccgatgc gcgggcggcg ctcgaacagc
     1861 tccggatgcg cgatgacgtg ccggtccggc agcccctgca gcaccgcgtc ggtgaggtcg
     1921 ccgcccaggt ccaccttggc gcgcggcgcg cggcgtcggt ccggcgccgg cggcttgtcc
     1981 gccgcgccgg gggtctcggc gctcatggcg tcgtggccgc gctgacgcgg cgctccagcc
     2041 agcggtgcag gtcgccggct acgcggtccg ggtcctcgat catcggcaca tggcccaggt
     2101 cctcgtagcg cacgtggtcc cagtccgggc ggtggcgcag cagcagggcg ccggtctccg
     2161 ggcggatgag ccggtcctgg gcgccctgca ggtacagccc gggcgcgccg atgcgggcga
     2221 cggcgtccca cacgcggttg cgccccacgg tgacggcgcg cacgacgtcc cgggcggcct
     2281 ggaggaacgc gcggtgcgcg tacggcatgg tgcgccggcg cgcggccagg tcggtgtggg
     2341 cgtccagcag ttcggcgtcc agggcctcgg gatcggcgac gcacagcgcc atggtctgct
     2401 ggacctgctg gcgcgggctc agccgccgct ggcgccgcgc gagcacggac tcgcccacgc
     2461 gcggcagcgc gaacgccgcg aagttcgcca ccacctgcgg gtccaggcgg gcgcccagcg
     2521 gcgcgggcac ggccgggttc accagcgcga tcccgcgcac cagctccggg tggcgcgccg
     2581 cgaggccgag cgagagcagc ccgcccatgg agttgcccac cagcacgacg gggccgccgg
     2641 gggccacggt ggccaggaag ccgcgcagca ggtcgccgtt gtcggccagg gtggcgcggc
     2701 gctccccgcc gtcggcggcg gccagcgggg tgaggccgaa gccggcgagg tccggcgccc
     2761 acaccgtgcc gacgtccggc agggcggcca gcagcggtcc gatgagccgc cagttcaggt
     2821 gggagccgcc caggccgtgc acgcacacca cggtgagggc gccgccggtg ccggccgcgc
     2881 cccaatgggc ggcgtggacg tcgtgggcgc cgggccccct gcgcagggcc gcccgctcca
     2941 cgcggatccg ctcggatcgc atccgcccgg cctagtcggc cggcgggatc tggagctcct
     3001 ggccgacccg cagcttgttg ttcttgtcga tcccgttggc gtcggcgatg aggttgaact
     3061 tggtgccgtc gccgtagacg tcctgggcga tggagatcag cgtgtccccg gacttgaccg
     3121 tgtaggtggt ccagccctcg cgggggtcgg cttcctcgtc ggtgtcggtg ggc
//
LOCUS       DATMKC010000177         8017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_170834, whole genome shotgun sequence.
ACCESSION   DATMKC010000177 DATMKC010000000
VERSION     DATMKC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170834"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1284
                     /locus_tag="VNU01_05185"
     CDS             259..1284
                     /locus_tag="VNU01_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH32046.1"
                     /translation="MSAVSLAPDRPVRRRTGVSVARALGSSTRAGIYEHLQQAAAALT
                     VREIAKAFDLHPNVARTHLEMLADAGLVVVGRRKHPGGGRPAKLYLARDEHDIPAQDA
                     DTPGPAGASQAPALLVRLLATLLDEPNRGSGMAQLARAHETAAAEGRRLVAGVGERDS
                     ADGVEAAAQTAVRALRPFAPEARVLKAGQDWVDLAGVRGLFRLLAAARPELADVLERG
                     LLSGVFAAAGVPVTLADAGPAPGGGSIWRARAVQAAPSRNAIEPSARIDARGLQREAG
                     VVEAMRAVTALRAGEILEVLAEGPGSPAAFARWADRAGHELLGVERAVDDTGRPGIRL
                     LIRKGAS"
     gene            1281..1592
                     /locus_tag="VNU01_05190"
     CDS             1281..1592
                     /locus_tag="VNU01_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWH32047.1"
                     /translation="MTVALLVSHTAAQGLALARAWAESGDDVVVVLLDGAAAAARDGH
                     ADAPALRAALAAGVAVSAHDDALRRRGVTGGGLTDGVKTIDLDEVADLVAEGADKVVW
                     L"
     gene            1589..1942
                     /locus_tag="VNU01_05195"
     CDS             1589..1942
                     /locus_tag="VNU01_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWH32048.1"
                     /translation="MSRRVAFVFTGTAPPEQSATALKLAERLLARGHRVTVFAHERDT
                     TLAAGTDELARAAAGLLRRGVHGGTLDWVVDAAAAERHGVAATQAPGVVPGDHSDLWA
                     FVRHADVVLSVGGGA"
     gene            1942..2319
                     /locus_tag="VNU01_05200"
     CDS             1942..2319
                     /locus_tag="VNU01_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWH32049.1"
                     /translation="MARTVVSLVRGTQGSARATEPALEANAYAVAEDLEVRVVLAGAA
                     VELALAGAEARPGELAGVALPPSASGQDLRGLIESGVAVLALADDLAARGIGRELLVD
                     GVETLQGADLAALLRTADAVLAW"
     gene            2331..3074
                     /locus_tag="VNU01_05205"
     CDS             2331..3074
                     /locus_tag="VNU01_05205"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWH32050.1"
                     /translation="MSLTVDVAGAARDLEEAPATEILRWAIAEVPRFAVSSSFGADSA
                     VLLHLLSTVDAGVPVLFLETGFHFPETLRYRRDLAALLGLTDVRDLRAQLTVAEQARE
                     HGGGLYVRDADACCAIRKVEPLNRALREFDGWASGVRREQTPERADTPVVQTVTREGR
                     DLVKVSPLARWSAQDVEDYLAAHALPRHPLAEAGYPSIGCAPCTRAVAPGEDPRAGRW
                     AGSAKTECGIHIELPGEAVPVQLSERSAR"
     gene            3071..3526
                     /locus_tag="VNU01_05210"
     CDS             3071..3526
                     /locus_tag="VNU01_05210"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HWH32051.1"
                     /translation="MKAQDERGKDVLDIDVRGPRFSAALTFAVLSAAYLLKSPGLLAF
                     QVVVFGVAAFAGLRWSPYGNVFRFLKRNLTFIGPPPATEPESGPRFSQLMGFLFSAAG
                     LALLTNGAETVGWSLVLVVVGLSALLAFTGICVGCEMYALGQRLRGKAA"
     gene            3530..4036
                     /locus_tag="VNU01_05215"
     CDS             3530..4036
                     /locus_tag="VNU01_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWH32052.1"
                     /translation="MAEALPLLLVVAAAALFSVWWKRRDGRIRDVEDRFTAEEVAAVG
                     APAGTAVLLEFTAPNCAPCRATKQVLEDVSALWPEVALVTIDVADALSVVKAHHVLRA
                     PTTFVLAPDGAVRGRISGVPDPGELADLLDGDGGPRPPRGRKLSADLHVETPSRARPR
                     WARRSRAA"
     gene            complement(4042..4398)
                     /locus_tag="VNU01_05220"
     CDS             complement(4042..4398)
                     /locus_tag="VNU01_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWH32053.1"
                     /translation="MAKRLVIKVTHAHDDPERAHIGVNVAATALASGLEAHLFLAAEG
                     VELARPGAAFDLADAPPVGDLLDAVYAGGSVTVCTPCATRRGYGEADFRDGTRLAGSA
                     AFVALLAEPDTTALVY"
     gene            4590..5750
                     /locus_tag="VNU01_05225"
     CDS             4590..5750
                     /locus_tag="VNU01_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32054.1"
                     /translation="MLDFSLSDEQEALYKAVADFGREVVSPLAGERDREGRFDRDVWD
                     RCGEMGLCGLPISAEYGGQGADAVTTGIAIEALAYGGHDAGLLLSLGAHLTIGSLPID
                     LHGTPEQKAKYLPKLTTGEWIGAFGITEPDAGSDTASIKSRGVRDGGDWVLNGTKTFI
                     TNGPICDVFTVVVRTNPDSGPGQGMTAFIVDRDTPGLSVGKHLDKMGNRSSPTSEMIM
                     EDVRVPDSQRLGDEGTALWKVAFECFDWERTVMIASGIGGMQASLERCVSYAKEREAF
                     GKPIGNFQAIGHKLAEMKIRLETARLALRHAAWLKDQGKPHMVEASIAKAYVAECAIK
                     NAEEAIQIFGGWGYIKEFPVERGWRDAKLSSIGGGTTEIQKLIISRVLLGEG"
     gene            5805..6305
                     /locus_tag="VNU01_05230"
     CDS             5805..6305
                     /locus_tag="VNU01_05230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH32055.1"
                     /translation="MLIRPATAADMAAVARIYDHYVLHTAITFDTEPKPVTEWEAGFA
                     RDVADGPYQFLVADVDGQVAAYAHTGQFHIRCAYWTSIFVSVYCAPERTGMGLGPALY
                     RTLFGEVLPGTAFHKVYAGVTQPNEASVKLHERFGFVREGLFAEVGQKFGQWHDVAWY
                     ARGLGG"
     gene            6433..7692
                     /locus_tag="VNU01_05235"
     CDS             6433..7692
                     /locus_tag="VNU01_05235"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWH32056.1"
                     /translation="MVHVHGRVVSERVTVGVPAWERVAVPVDVGKHTAMAMVADFAGE
                     RLVAPFVFPLDRRGIGELVDRVARVVGERGVSRVEVGVEAAGHYHRPVTASGLLPADW
                     QLFELNPAHVTEQRRVLGKRGVKTDQIDLVAIYDLLVAGRGLAAGRRDAALLELQAWT
                     AMRFRRVTAVIAAKNQLLGQMDRAFPGAGRCITASLCDTKIGRLVLAEFSDPTRLARM
                     GAERFRRFAAHRGVIVQRKVAQRFVAAAKDAVPLDGAATARRLLTADLALLETLERHV
                     ADAEAELARILPATPFQVLTTTPGWGLVRAARYGAALGDPARWPTARQVYRAAGLSPR
                     VYESAGRRVDGAITREGSVELRGALLELGMGLWLCDPVTRPAVAALRARGKPAGVIAC
                     ALANRANRIAFAMVRDQLAYDPDRWRT"
BASE COUNT         1043 a         2848 c         3031 g         1095 t
ORIGIN      
        1 cccccggggc ggttccgcgg ccggccgggc cccgtcctcc ggccccgccg gcctgctgct
       61 cgatgccttc gacgggttgc tgctgggagc gccggtcgcc tacgtgctgg cccgctccgc
      121 cgcgctgtag gcagtaaaca ctgtttacac tagtctgagg cgcgttcctg cgcggttttc
      181 cctatcggaa cggttgtcgt agggaaaacc acgccgtaca accgtcgcca gaccgaagtt
      241 ttcgacgagg aggagcgcgt gagcgccgtg tcgctggccc ctgaccgtcc ggtgcgccgc
      301 cggactggcg tcagcgtggc ccgcgcgctc ggctcctcca cccgcgcggg catctacgag
      361 cacctgcagc aggccgcggc ggcgctgacc gtgcgcgaga tcgccaaagc gttcgacctg
      421 caccccaacg tggcccggac gcacttggag atgctcgccg acgcgggact ggtggtggtc
      481 gggcggcgca agcaccccgg cggcggccgc ccggccaagc tctacctggc gcgcgacgag
      541 cacgacatcc ccgcccagga cgcggacacg cccggacccg ccggcgcctc gcaggcgccg
      601 gcgctgctgg tccggctgct cgccacgctg ctggacgagc cgaaccgcgg cagcggcatg
      661 gcgcagctcg ccagagcgca cgagaccgcc gccgccgaag ggcggcggct cgtggccggc
      721 gtgggcgagc gcgacagcgc cgatggcgtg gaggcggccg cgcagacggc cgtgcgggcg
      781 ctgcgcccgt tcgcgccgga ggcccgtgtg ctgaaggcgg gccaggactg ggtcgacctc
      841 gccggcgtgc ggggcctgtt ccgcctgctg gccgccgccc ggccggagct ggccgacgtg
      901 ctcgagcgcg gcctgctgtc gggggtgttc gcggcggcgg gcgtgccggt gaccctggcc
      961 gacgccggcc cggcgccggg gggcgggtcg atctggcgcg cccgcgccgt ccaggccgcg
     1021 cccagccgca acgccatcga gccgtccgcg cgcatcgacg ctcgcggcct gcagcgcgag
     1081 gccggcgtcg tcgaggcgat gcgcgccgtc acggcgctgc gcgcgggcga gatcctggaa
     1141 gtcctggccg aaggccccgg ctcgcccgcg gcgttcgccc gctgggccga ccgggccggc
     1201 cacgagctgc tcggtgtgga gcgcgccgtc gacgacaccg gccggccggg catccggctg
     1261 ctgatccgca agggcgcgtc atgacggtcg ccctgctggt gtcccacacg gccgcgcagg
     1321 gcctcgcgct ggcgcgcgcc tgggccgagt cgggcgacga cgtcgtcgtg gtcctgctgg
     1381 acggcgcggc cgcggcggcg cgtgacgggc acgccgacgc gcccgcgctg cgcgccgccc
     1441 tggccgccgg ggtggcggtc tccgcgcacg acgacgccct gcgccgtcgc ggcgtgaccg
     1501 gcggcgggct gaccgacggg gtcaagacca tcgacctgga cgaggtggcg gacctggtgg
     1561 cggagggcgc ggacaaggtg gtgtggctgt gagccgccgt gtcgcgttcg tgttcacggg
     1621 cacggcgccg cccgagcagt cggccacggc gctgaagctg gcggagcgcc tgctcgcgcg
     1681 cggccatcgc gtcaccgtgt tcgcgcacga acgcgacacc acgctggccg ccggcaccga
     1741 cgagctggcg cgcgccgccg cgggcctgct gcggcggggc gtgcacggcg ggacgctgga
     1801 ctgggtcgtg gacgccgccg ccgccgagcg ccacggcgtc gcggcgacgc aggcgcccgg
     1861 cgtggtgccg ggcgaccact cggacctgtg ggcgttcgtc cgtcacgccg acgtggtgct
     1921 gtccgtggga ggtggcgcct gatggccagg acggtcgtga gcctggtgcg cggcacgcag
     1981 ggctcggccc gtgccacgga gcctgcgctg gaggccaacg cctacgcggt cgccgaggac
     2041 ctcgaggtcc gcgtggtgct cgccggcgcg gcggtggagc tggcgctggc gggagccgag
     2101 gcgcgccccg gcgagctcgc gggcgtcgcg ctgccgccgt ccgcgagcgg gcaggacctg
     2161 cgcgggctga tcgagagcgg ggtggcggtg ctggcgctgg cggacgacct ggccgcgcgc
     2221 ggcatcggcc gcgagctgct ggtggacgga gtggagacgc tccagggcgc ggacctggcg
     2281 gcgctgctgc gcaccgccga cgcggtgctg gcgtggtgaa ggagatcgac gtgagcttga
     2341 ccgtggacgt cgccggcgcg gcccgggacc tcgaggaggc cccggccacc gagatcctgc
     2401 gctgggcgat cgccgaggtg ccgcgcttcg cggtgtcgtc cagcttcggc gcggacagcg
     2461 ccgtgctgct gcacctgctg tccaccgtcg acgccggcgt gcccgtgctg ttcctggaga
     2521 ccggcttcca cttcccggag acgctgcgct accggcgcga cctggccgcg ctgctcggcc
     2581 tgaccgacgt gcgcgacctg cgggcgcagc tcaccgtggc cgagcaggcg cgcgagcacg
     2641 gtggcggcct gtacgtgcgc gacgccgacg cctgctgcgc catccggaag gtggagccgc
     2701 tgaaccgcgc gctgcgcgag ttcgacggct gggcctcggg cgtgcgccgc gagcagaccc
     2761 cggagcgtgc cgacacgccc gtggtgcaga cggtgacgcg ggagggccgc gacctggtga
     2821 aggtgtcccc gctcgcgcgc tggagcgcgc aggacgtcga ggactacctg gcggcgcacg
     2881 cgctgccgcg ccacccgctg gcggaggcgg gctacccctc catcggctgt gcgccgtgca
     2941 cgcgcgcggt cgcgccgggc gaggaccccc gggcgggccg ctgggcgggc agcgccaaga
     3001 cggaatgcgg gatccacatc gagctgccgg gcgaggccgt ccccgtgcag ctctcggaaa
     3061 ggagtgcgcg atgaaggcac aggacgaacg cggcaaggac gtgctcgaca tcgacgtgcg
     3121 cgggccgcgc ttcagcgccg cgctcacgtt cgcggtgctg tccgccgcct acctgctcaa
     3181 gtcgccgggc ctgctggcct tccaggtggt cgtcttcggc gtggcggcgt tcgcggggct
     3241 gcgctggtcg ccgtacggca acgtgttccg cttcctcaag cgcaacctca cgttcatcgg
     3301 gccgccgccc gccaccgagc ccgagagcgg cccccgcttc agccagctca tgggtttcct
     3361 gttctccgcg gccggcctcg cgctgctcac caacggtgcg gagaccgtcg gctggagcct
     3421 ggtgctggtg gtcgtcggcc tgtcggcgct gttggccttc accggcatct gcgtgggctg
     3481 cgagatgtac gcgctggggc agcggctgcg gggcaaggcg gcctgaggaa tggccgaggc
     3541 gctcccgctc ctgctcgtcg tcgccgcggc ggcgctgttc agtgtgtggt ggaagcgccg
     3601 cgacggccgc atccgtgacg tcgaggaccg cttcaccgcc gaggaggtcg ccgccgtcgg
     3661 cgcgcccgcc gggaccgcgg tactgctgga gttcaccgcg ccaaactgcg cgccctgccg
     3721 cgccacgaag caggtgctcg aggacgtctc cgcgctgtgg ccggaggtgg cgctggtcac
     3781 catcgacgtc gccgacgcgc tgagcgtggt gaaggcgcac cacgtgctgc gcgcccccac
     3841 cacgttcgtg ctggcgcccg acggcgccgt gcgcgggcgc atcagcggcg tgccggaccc
     3901 gggtgagctg gccgacctgc tggacggcga cggcggcccc cggccgccgc gcgggcgcaa
     3961 gctcagcgcg gacctccacg tggagacgcc cagccgggca cgcccgcgct gggctcgccg
     4021 gtcccgcgcg gcctagagca gtcagtagac cagcgcggtg gtgtcgggtt cggcgagcag
     4081 ggcgacgaag gcggcggagc cggccaggcg ggtgccgtcg cggaagtcgg cctcgccgta
     4141 gccccggcgg gtcgcgcacg gcgtgcacac ggtgacgctg ccgccggcgt agaccgcgtc
     4201 cagcaagtcg ccgacgggag gggcgtcggc caggtcgaac gccgcgccgg ggcgcgcgag
     4261 ctcgacgccc tccgcggcca ggaacaggtg cgcctccagg ccgctggcca gcgcggtggc
     4321 ggccacgttg acaccgatgt gcgcgcgctc gggatcgtcg tgggcgtgcg tgaccttgat
     4381 gacgagtcgc tttgccatgt ccgctagcct aggcggcatg aacggagtcg aactcttctt
     4441 cacgggcctg ctggtcacca tgacgggcgt gggcggcctc ttcggcctct acgtcatctg
     4501 gcgcctgaag ggctgagccg cctccgctcg ctccgaggcg gcggaacgga taccgtgtaa
     4561 tcgttccgtt ccgacccgtg gaggcaggcg tgctggactt ctccctttcc gacgagcaag
     4621 aggcgctgta caaggccgtc gccgacttcg gccgcgaggt cgtgtcgccg ctggccggtg
     4681 agcgcgaccg ggaggggcgc ttcgaccggg acgtctggga ccgctgcggc gagatgggcc
     4741 tgtgcggcct gccgatctcg gcggagtacg gcggccaggg cgccgacgcc gtcaccacgg
     4801 gcatcgcgat cgaggccttg gcctacggcg gccacgacgc cgggctgctg ctgtcgctcg
     4861 gcgcgcatct caccatcggg tcgctgccca tcgacctgca cggcacgccg gagcagaagg
     4921 cgaagtacct gcccaagctg accaccggcg agtggatcgg cgcgttcggc atcaccgagc
     4981 ccgacgccgg cagcgacacg gcgagcatca agagcagggg cgtgcgcgac ggcggcgact
     5041 gggtgctcaa cggcaccaag acgttcatca ccaacgggcc catctgcgac gtgttcaccg
     5101 tggtggtgcg caccaacccg gactccggcc cggggcaggg catgaccgcc ttcatcgtcg
     5161 accgggacac gcccggcctg tccgtgggca agcacctgga caagatgggc aaccgcagct
     5221 cgcccacgtc cgagatgatc atggaggacg tgcgcgtgcc ggactcccag cgcctcggcg
     5281 acgagggaac ggcgctgtgg aaggtggcct tcgagtgctt cgactgggag cgcaccgtca
     5341 tgatcgccag cggcatcggc ggcatgcagg cgagcttgga gcgctgcgtc tcctacgcca
     5401 aggagcgtga ggcgttcggc aagccgatcg gcaacttcca ggccatcggg cacaagctcg
     5461 ccgagatgaa gatccgcctg gagacggccc gactggccct gcgccacgcg gcctggctca
     5521 aggaccaggg caagccccac atggtcgagg cgtccatcgc caaggcctac gtcgccgagt
     5581 gcgccatcaa gaacgccgag gaggcgatcc agatcttcgg cggctggggc tacatcaagg
     5641 agttccccgt ggagcggggc tggcgcgacg cgaagctgag ttccatcggc gggggcacca
     5701 cggagatcca gaagctcatc atcagccgcg tcctgctcgg cgaaggctga tccgcgagcg
     5761 acaatgaggg catgacgcag ccctcccgca caggcgccgc accgatgctg atccgtcccg
     5821 cgaccgcggc cgacatggcc gcggtcgcgc gcatctacga ccactacgtc ctgcacaccg
     5881 cgatcacgtt cgacaccgag cccaagccag tgaccgagtg ggaggccggg ttcgcccggg
     5941 acgtcgccga cgggccgtac cagttcctgg tcgccgacgt cgacgggcag gtggcggcct
     6001 acgcgcacac cggccagttc cacatccgct gcgcgtactg gacgtcgatc ttcgtgtccg
     6061 tgtactgcgc gccggagcgc accggcatgg ggctcgggcc ggcgctgtac cggacgctgt
     6121 tcggcgaggt gctgcccggc accgccttcc acaaggtcta cgccggcgtc acgcagccca
     6181 acgaggcgtc ggtgaagctg cacgagcgct tcggcttcgt gcgcgagggc ctgttcgccg
     6241 aggtcggcca gaagttcggc cagtggcacg acgtcgcctg gtacgcccgc gggctgggcg
     6301 gctgatatag gtcacggagc ggttccttgg tgaacacctc cggaggtgcc agaagggacc
     6361 cgtaagggtt cgttgagggt cggggcgttg ccacgcccgt ggtcctcctg acaccttccg
     6421 gaggtgtttt tcatggtgca cgttcatggc cgggttgtct cggaacgggt cacggtcgga
     6481 gtgccggcgt gggagcgggt cgcggtgccg gtggatgtcg gcaagcacac ggcgatggcg
     6541 atggtcgcgg atttcgccgg cgagcggctg gtggcgccgt tcgtgtttcc gctggatcgg
     6601 cggggcatcg gcgagttggt cgaccgggtc gcgcgtgtgg tcggcgagcg cggcgtttcg
     6661 cgggtcgagg tcggtgtgga ggccgcgggg cattaccacc ggccggtgac ggcctcgggg
     6721 ctgttgccgg ccgactggca gctgttcgag ctgaacccgg cgcacgtcac ggagcaacgc
     6781 cgcgtgctgg gcaagcgtgg cgtcaagacc gaccagatcg acctggtggc gatctatgac
     6841 ctgctggtgg ccggccgtgg ccttgcggcc ggacggcgcg acgcggcgct gctggagttg
     6901 caggcgtgga ccgcgatgcg ctttcggcgg gtcacggcgg tgatcgcggc gaagaaccag
     6961 ctgttggggc agatggaccg cgcgttccca ggagcgggca ggtgcatcac ggcgagcttg
     7021 tgcgatacga agatcggccg gctggtcctg gccgagttct ccgacccgac gcggctcgcg
     7081 cgaatgggcg cagagcggtt ccgccgcttc gccgcccacc gtggcgtgat cgtgcagcgc
     7141 aaggtcgccc agcggttcgt cgccgccgcg aaagacgcgg tgccgctgga cggcgccgcg
     7201 accgcccggc ggctgctgac cgccgatctg gcgctgttgg aaaccctgga gcgccacgtc
     7261 gccgacgccg aagccgaact cgcccggatc ctgcccgcca ccccgttcca ggtgctgacc
     7321 accacaccgg gctggggact ggtccgcgcg gcacgctacg gcgccgcgct cggtgacccg
     7381 gcccgctggc cgaccgcccg gcaggtctac cgcgccgccg gactcagccc gagggtgtat
     7441 gagtccgccg gccgccgcgt cgacggggcc atcacccgcg aaggctccgt cgagctgcgc
     7501 ggcgcgctgc tcgaactcgg catgggcctg tggctgtgtg acccggtcac ccgtcccgcc
     7561 gtggccgcgc tgcgagcacg cggcaagccc gccggcgtca tcgcgtgcgc gctggcaaac
     7621 cgcgccaacc gcatcgcgtt cgcgatggtc cgcgaccaac tcgcctacga ccccgaccgc
     7681 tggaggacct gaaccagcct tcttcacaac cccgctcgga cgtgacacga tcagaggctg
     7741 gaggccggat cagacaccgc cacggctatg gcggaccgcc acggcggtca cgccgcttat
     7801 tagcttggtg cccggcctcc acccgtgtcc ggagctcgga caaggccagc tgacccgacc
     7861 ccgacggtcg aggtcgcata cgtcagcatg agcccgccac ggcacccgcc atgacgccga
     7921 acgcccaagc gtccagaccc ctgccgcatc ccggctcaca caggcgggac aatcccacac
     7981 gtgccagcac ccggttgaca agacctacgg ccagcta
//
LOCUS       DATMKC010000178         4683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_170805, whole genome shotgun sequence.
ACCESSION   DATMKC010000178 DATMKC010000000
VERSION     DATMKC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170805"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VNU01_05240"
     CDS             <1..696
                     /locus_tag="VNU01_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32057.1"
                     /translation="LAPDAGVLDQLRAGDAVAVVATDLGYVADAPAATHLLAIGEREV
                     RLVGPGEFEVRSRESLDAHRPVGSVTLTRPGEEIGAADRARGILRTSGVVSIAHEMVG
                     GAATCLALSLDYVRAREQFGHPVGAYQAVSHQCVDMFISIEVARSLATYAAWAVSEGH
                     PEQEVAASRAHVAAYEAARLCAERTIQVHGGMGMTHEHSAHRFLTRAITTAKVLQPVR
                     ASRHMLAARLAAP"
     gene            778..1545
                     /locus_tag="VNU01_05245"
     CDS             778..1545
                     /locus_tag="VNU01_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH32058.1"
                     /translation="MTSLVDRFRLDGRVAIVTGASAGLGRQIAIGLGQAGAAVVLAAR
                     RAEALGETAELVRATGARAAEVPCDITSPPDCDRLAAAAVDTFGRLDILVNNAGIGHA
                     VPATREAPEQFEQVMSVNVLGAYWMAQACARRMGSGASIVNIGSVLGLTTAGLPQAAY
                     TASKAALIGLTLDLAQQWTGRKGIRVNTIAAGFFDSGMALEHADGYLDEIVARRIPAG
                     RLGQPEELAAVVVFLAGDAASYVSGSVLSVDGGLLVT"
     gene            1581..2624
                     /locus_tag="VNU01_05250"
     CDS             1581..2624
                     /locus_tag="VNU01_05250"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWH32059.1"
                     /translation="MSEQIGTKALQQAERPLPDEVIPYGWYHVGWSAELNAEDVVPLA
                     YFDRHLVMYRGTSGEVHVLDAHCAHLGAHMGYGGKVSCEDIVCPFHGWRWNHEGENVE
                     VPYSDRPSARRIGAWPVAERNGCIFVWYHPQGGEPLSEPPVMEEESPDSYFPVHPDGA
                     MLWSVTFPPQYAAENLVDFAHFKYVHAAATAATLEGFEVQGPHFTSVLGLLMGEGKES
                     TWMTPTGPTPAKLVTRVFGPGVNTVRFEVSRPGIADVIVMTAVTPETLSPCKGDLRIS
                     ILVRAEDLSGQGRDPAWVASRWYAEERRQVDQDVMIWNNMRYLEKAPFPPSEFKAFNA
                     MRRWSRQFYLVER"
     gene            2621..3781
                     /locus_tag="VNU01_05255"
     CDS             2621..3781
                     /locus_tag="VNU01_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWH32060.1"
                     /translation="MTASLKDQVAIVGVGETEYRRAHDRPVQELILDAIGTALDDAGL
                     SGSDVDGIVTEGAIVEGLLSIEEVAATIGVRELSYGAKFGIGGAGTVASPLHAAMAVS
                     HGLADVVLCYFGVDWGSAAAGPYGFHEQYPEKRHLEIPHGYFGQPVYFGALTHRYMAE
                     YGLTKEQMGALAVGTRGWARLNPSAMRRDEMTMGDYLASPVVATPLSVADCSLISDGA
                     AAFVMTRADRALDSPAPRILVSGAAYARNNMSPGAALTQDPAYLSTMARVTGPRALRM
                     AEMRIEDIDLFEIYDCFTITCLMQLEDLGLCDKGEIGALVAEAGTGPGGRLPVNTHGG
                     LLSHAYVLGVNHVAEAVHQLRNTAGARQVEARTALVTGYGGPDHCTLILTRG"
     gene            3787..4395
                     /locus_tag="VNU01_05260"
     CDS             3787..4395
                     /locus_tag="VNU01_05260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH32061.1"
                     /translation="MNAWPDDDAWRMPAEPGAGGEGFGRLVAAVRDLQDAITAARLSS
                     PEAHAVAARLEAVLEDLDAFAVPEELQIAGRRPDLPGRGQFFAPVIRWEVLTGEGVTA
                     WVRFGRYYLGANSAAHGGAIPLVFDEVMGEVANRDRPRSRTAYLKVDYRRITPVGRDL
                     RVEAAVVRQEGRKRFVEGAIYDDADLLCEATALFVELRPGQP"
     gene            complement(4427..>4683)
                     /locus_tag="VNU01_05265"
     CDS             complement(4427..>4683)
                     /locus_tag="VNU01_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32062.1"
                     /translation="LEERVLLRVEDANVGSILGIGFPSWTGGAAQFVDQYPGGVGGFV
                     QRCESLAAGYGERFRATSSALSAAAAGETLRAHFARGVTV"
BASE COUNT          687 a         1600 c         1737 g          659 t
ORIGIN      
        1 ctggcgcccg atgccggcgt gctggaccag ctgcgcgcgg gggacgccgt cgcggtggtc
       61 gccaccgacc tgggctacgt cgccgacgcg cccgcggcca ctcacttgct cgccatcggc
      121 gagcgcgagg tgcgcttggt gggtcccggc gagttcgagg tgcggtcgcg cgagtcactg
      181 gacgcccacc gccccgtggg gtcggtgacc ctcacgcggc cgggtgagga gatcggtgcg
      241 gccgaccggg cgcgggggat cctgcggacc agcggcgtgg tgtccatcgc ccacgagatg
      301 gtggggggcg cggccacctg cctggcgctc agcctggact acgtacgcgc acgcgagcag
      361 ttcggccacc cggtgggggc ctaccaggct gtcagccacc agtgcgtgga catgttcatc
      421 agcatcgagg tcgcgcgcag cctcgccacc tacgcggcgt gggcggtgag cgagggtcat
      481 cccgagcagg aagtcgccgc gagccgcgcg cacgtggcgg cgtacgaagc ggcgcggctg
      541 tgcgccgagc ggacgatcca agtacacgga ggcatgggca tgacccacga gcacagcgcc
      601 catcgattcc tcacgcgcgc catcaccacc gccaaggtgc tgcagccggt gcgcgcgtca
      661 cggcacatgc tggcggcgcg gctggcggcg ccatagccgc ccgccccggc ggccgcaggc
      721 cgccacccga gacatgacac agcggcaagg actggccgag tggaagacgc gcagcaggtg
      781 acttcgttgg ttgaccgctt ccgcctggac gggcgggtgg cgatcgtgac cggcgcgtcc
      841 gccgggctgg gacgccagat cgccatcggc ctcggtcagg cgggcgcggc cgtggtcctg
      901 gcggcgcgca gggccgaagc cctgggggag accgccgagc tggtgcgggc gaccggcgcg
      961 cgcgccgccg aggtgccgtg cgacatcacc agcccgccgg actgcgaccg gctcgccgca
     1021 gcggcggtgg acacgttcgg gcgactggac atcctggtga acaacgccgg tatcggccac
     1081 gccgtaccgg ccacccgcga ggccccggag cagttcgagc aggtgatgtc ggtgaacgtg
     1141 ctcggcgcgt actggatggc gcaggcgtgc gcacgacgca tgggttccgg cgcctccatc
     1201 gtcaacatcg gcagcgtgct cggcttgacg accgccggac tgccccaggc cgcgtatacg
     1261 gccagcaagg ccgcattgat cggcctcacg ctggacctgg cgcagcagtg gaccgggcgc
     1321 aagggcatcc gtgtcaacac gatcgcggcc ggtttcttcg actccggcat ggcgctggag
     1381 cacgccgacg gatacctgga cgagatcgtc gcccgccgga taccggccgg gcgcctgggg
     1441 cagccggagg agctggccgc cgtcgtggtg ttcctggccg gggacgcggc cagctacgtc
     1501 agcggcagcg tcttgagcgt ggacggcggg ctgctcgtca cctgacgcgc agcccagtga
     1561 aacgatccga ggagcagcag atgagcgagc agatcggcac caaggcgctc cagcaggcgg
     1621 agcggccgct tccggacgag gtcatccctt acggctggta ccacgtcggc tggtcggcgg
     1681 agctcaatgc ggaggatgtg gtgccgctgg cctatttcga ccgccacctc gtgatgtacc
     1741 gcggcacctc cggcgaggtg catgtgctcg acgcccactg cgcccacctc ggcgcacaca
     1801 tgggctacgg cggtaaggtc tcctgcgagg acatcgtgtg cccgttccac ggctggcgct
     1861 ggaaccacga gggcgagaac gtggaggtgc cctacagcga ccggcccagt gcgcgacgca
     1921 tcggcgcatg gccggtggcc gaacgcaacg gctgcatctt cgtgtggtac cacccgcagg
     1981 gcggtgagcc gctgtcggag cccccggtga tggaagagga gtccccggac agctacttcc
     2041 cggtgcaccc ggacggcgcg atgctgtggt cggtgacctt cccgccgcag tacgcggccg
     2101 agaacctggt ggacttcgca cacttcaagt acgtgcacgc cgccgccacg gccgcgaccc
     2161 tggagggctt cgaggtccaa ggcccgcact tcacctccgt gctgggcctg ctcatggggg
     2221 agggcaagga gtccacttgg atgacgccca ccggcccgac cccggccaag ctggtgacgc
     2281 gggtgttcgg tcccggtgtg aatacggtgc gcttcgaggt gagccggccc ggcatcgccg
     2341 acgtgatcgt catgacggcg gtgacgccgg agacgctgtc gccgtgcaag ggcgacttgc
     2401 gcatctccat cctggtgcgc gcggaggact tgtccggaca gggccgcgac ccggcgtggg
     2461 tcgcgagccg ctggtatgcc gaggagcggc gacaggtaga ccaggacgtg atgatttgga
     2521 acaacatgcg ctacctggag aaggcgccgt tcccgccgag tgagttcaag gccttcaacg
     2581 ccatgcgccg ctggtcgcgc cagttctact tggtggagcg gtgaccgcgt cgttgaagga
     2641 ccaggtcgcc atcgtcggcg tgggcgagac ggagtaccgg cgcgcacacg accggcctgt
     2701 gcaagagctg atcctggacg cgatcggcac cgcgctggac gacgcgggcc tgagcgggtc
     2761 cgacgtggac ggcatcgtga cggagggcgc catcgtcgag gggctgctca gcatcgagga
     2821 ggtggccgcg accatcggcg tgcgcgagtt gtcctacggc gccaagttcg gcatcggggg
     2881 cgcggggacc gtcgcctccc cgctgcacgc cgcgatggcg gtctcccacg gcttggccga
     2941 cgtggtgctg tgctatttcg gcgtggattg gggcagcgcc gccgccggcc cctacggctt
     3001 ccacgagcag taccccgaga agcggcacct ggagatccct cacggctact tcgggcagcc
     3061 ggtgtacttc ggcgccctga ctcaccgcta catggccgag tacggtctga ccaaggagca
     3121 gatgggcgcg ctggcggtgg gcacgcgcgg ctgggcgcgg ctgaatccct ccgccatgcg
     3181 gcgcgacgag atgacgatgg gcgactacct ggcctcgccg gtggtcgcga cgccgctgtc
     3241 ggtcgccgac tgcagcctca tcagcgacgg cgccgccgcc ttcgtcatga cccgggcgga
     3301 tcgcgcgctg gacagcccgg ctcctcggat cctcgtgtcg ggcgcggcgt acgcgcgcaa
     3361 caacatgtcg ccgggcgccg cgctgactca ggacccggcc tacctgtcca ccatggcccg
     3421 ggtgaccggt ccccgcgcgc tgcgcatggc cgagatgcgc attgaggaca tcgacctgtt
     3481 cgagatctac gactgcttca ccatcacctg cctgatgcag ctggaggatc tgggcctgtg
     3541 cgacaagggc gagatcgggg cgctggtcgc cgaggccggc accggtccgg gcggaagact
     3601 gccggtcaac actcacgggg ggttgctgtc gcacgcgtac gtgctcgggg tcaatcacgt
     3661 cgccgaggcc gttcaccagt tgcgcaacac cgcgggtgcg cggcaggtgg aggcacgcac
     3721 cgcgctggtg accggctacg ggggaccgga ccactgcacc ctgatcctga ccaggggctg
     3781 agggcggtga acgcatggcc tgacgacgac gcctggcgta tgcccgccga gccgggcgcc
     3841 ggaggtgagg gcttcggccg cctggtggcg gcggtgcgtg acctgcagga cgcgatcacc
     3901 gccgcgcgcc tcagttcacc ggaggcgcac gcggtcgccg cccgcctgga agcggttctc
     3961 gaggatctgg acgccttcgc ggtgcccgag gagctgcaaa tcgcggggcg ccggccggac
     4021 ttgcccggca gggggcagtt cttcgcgcct gtcatccgat gggaggttct caccggcgaa
     4081 ggtgtcacgg cctgggtccg cttcggccgc tactacctgg gcgccaacag cgccgcgcac
     4141 gggggggcca ttccgctggt gttcgacgag gtgatgggcg aggtcgccaa ccgcgaccgc
     4201 ccgcgctccc gcaccgccta cctgaaggtg gactaccggc gcatcacgcc cgtgggaagg
     4261 gatctgcgcg tggaggctgc cgtggtccgc caggaggggc gcaagcgctt cgtcgaaggg
     4321 gccatctacg acgacgccga tctcctgtgc gaggccacgg ccctgttcgt ggagctgcga
     4381 cccggacagc cgtgagccgg cggcgccggc gcgccgccgg cagtcgtcac accgtgacgc
     4441 cccgggcgaa gtgcgcgcgc agcgtctccc cggctgcggc cgcggacagc gcggaggagg
     4501 tggcgcggaa gcgctcgccg tagcccgcgg ccaggctctc gcaccgctgg acgaaaccgc
     4561 cgaccccgcc ggggtactga tcgacgaact gcgcggcgcc gccggtccaa ctcgggaagc
     4621 cgatgcccag gatggagccc acgttggcgt cctcgacgcg cagcagcacg cgctcctcca
     4681 ggc
//
LOCUS       DATMKC010000179         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_259462, whole genome shotgun sequence.
ACCESSION   DATMKC010000179 DATMKC010000000
VERSION     DATMKC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259462"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNU01_05270"
     CDS             complement(<1..773)
                     /locus_tag="VNU01_05270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWH32063.1"
                     /translation="MSRHARRATRALLAAVLVLPLAACGGAVGDPDAAAVVNGAAIPL
                     DTVAQRYESVRRDPQLAQQLAADEDGSFKARVQARLLTQMIQAELLSQAAEDIGIEIT
                     DADIEAERQSIIEQVGGQEAFDQIIEENALSERDVRDQIRDLVVEREVEEALGADVQV
                     SDADVRAFYEENRATRYDRVRASHILVPTREEAERVMERLQAGEDFAALAQELSQDPG
                     SAQQGGDLGEFTRGRLVPEFEEAAFGAEVGELVGPIQTLS"
     gene            complement(846..>2277)
                     /locus_tag="VNU01_05275"
     CDS             complement(846..>2277)
                     /locus_tag="VNU01_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HWH32064.1"
                     /translation="ISVLSRFSGQREQKAILDGLAAGTVDLVIGTHRLLGADVVFKDL
                     GLVVVDEEQRFGVGHKEKLKALRTSVDVLTMSATPIPRTMEMAITGIRDLSTIDTPPE
                     ERRPIVTQVQPWEEGLAALAVRRELLREGQVFWVHNRVETIEAAAERVRELVPGARVG
                     VAHGQMDEAALERVMLAFWNREFDVLVSTTIIESGLDIPNANTLIIERADLLGLAQLY
                     QLRGRVGRSSERGYAYLFYPAHERMTEEAHKRLETLATYTGLGSGMSIALKDLEIRGA
                     GNLFGPDQSGQVAAVGFDEYARLMREAIEDFKLGKRAPQVHEPEPEITIDLPVDAHLP
                     HDWITDEALRLEAYRKVAAVRDAAGVKAVREELVDRYGALPPAAERLLSLAALKAALR
                     RWGVRELTTTPRRTIRVAPLQLADFQEVRLGRIAKDALYNAPARALELPMPRDGDLIA
                     YVARRLKDVFAPAERPGASSAPAKAR"
BASE COUNT          288 a          842 c          809 g          338 t
ORIGIN      
        1 gacagggtct ggatggggcc gaccagctca ccgacctcgg cgccgaacgc ggcctcctcg
       61 aattccggca ccagccgccc gcgcgtgaac tcgcccaggt cgccaccctg ctgcgcgctg
      121 ccggggtcct gcgacagctc ctgggccagc gcggcgaagt cctcgcccgc ctggagccgc
      181 tccatgacgc gctcggcctc ctcgcgcgtg ggcaccagga tgtggctggc ccgcacgcgg
      241 tcgtagcgcg tggcgcggtt ctcctcgtag aaggcgcgca cgtccgcgtc gcttacctgg
      301 acgtcggcgc cgagcgcctc ctccacctcg cgctccacca cgaggtcgcg gatctggtcg
      361 cgcacgtccc gttccgacag cgcgttctcc tcgatgatct ggtcgaacgc ctcctggccc
      421 cccacctgct cgatgatgct ctggcgctcc gcctcgatgt cggcgtcggt gatctcgatg
      481 ccgatgtcct cggcggcctg gctgagcagc tcggcctgga tcatctgcgt gagcagccgc
      541 gcctgcacgc gcgccttgaa gctgccgtcc tcgtccgcgg cgagctgctg ggccagctgc
      601 gggtcacggc gcacggactc gtagcgctgg gcgacggtgt caagcgggat cgcggcgccg
      661 ttgacgacgg cggcggcgtc cgggtcgccg accgcgccgc cacaggcggc cagcggcagc
      721 accagcacgg cggcgagcag cgcgcgcgtg gcgcggcggg cgtgacggga catggcttca
      781 gtgggcacgg gcgtggtccg tcctaggcga atctgggcgg gagtctgaag gatgtggctg
      841 gaagtctacc gagccttcgc gggagccgaa ctcgccccag ggcgctccgc gggcgcgaac
      901 acgtccttga ggcggcgcgc cacgtaggcg atcaggtcgc catcgcgcgg catcggcagc
      961 tccagggccc gggcgggcgc gttgtacagc gcgtccttgg cgatgcggcc cagccgcacc
     1021 tcctggaagt cggcgagctg cagcggtgcc acgcggatcg tgcgccgcgg cgtggtggtg
     1081 agctcccgca cgccccagcg ccgcagggcc gccttgagcg ccgccagcga cagcagccgc
     1141 tcagccgccg gcggcagcgc gccgtagcgg tccaccagct cctcgcgcac cgccttcacg
     1201 ccggccgcgt cgcgcaccgc cgcgaccttg cggtaggcct ccaggcgcag cgcctcgtcg
     1261 gtgatccagt cgtgcggcag gtgggcgtcc accggcaggt cgatggtgat ctccggctcg
     1321 ggctcgtgca cctgcggcgc gcgcttgccc agcttgaagt cctcgatcgc ctcgcgcatc
     1381 agccgcgcgt actcgtcgaa gccgaccgcg gcgacctggc cggactggtc cgggccgaac
     1441 aggttgccgg cgccgcggat ctccaggtcc ttcagcgcga tggacatgcc ggagcccagg
     1501 ccggtgtagg tggccagcgt ctccaggcgc ttgtgcgcct cctccgtcat gcgctcgtgc
     1561 gccgggtaga acaggtaggc gtagccgcgc tcgctggagc gccccacgcg gccgcgcagc
     1621 tggtagagct gcgccaggcc cagcaggtcg gcgcgctcga tgatcagcgt gttggcgttg
     1681 gggatgtcca ggccgctctc gatgatggtg gtggacacca gcacgtcgaa ctcgcggttc
     1741 cagaaggcca gcatcacgcg ctccagcgcc gcctcgtcca tctgcccgtg cgccacgccg
     1801 acgcgcgcgc cgggcaccag ttcgcgcacg cgctcggcgg cggcctcgat ggtctcgacg
     1861 cggttgtgca cccagaacac ctggccctcg cgcaagagct cccgccgcac cgccagcgcg
     1921 gccaggccct cctcccacgg ctggacctgc gtgacgatcg ggcggcgctc ctccggcggc
     1981 gtgtcgatgg tggacaggtc gcggatgccg gtgatggcca tctccatggt gcgcgggatg
     2041 ggggtggcgc tcatggtcag cacgtccacc gacgtgcgca gcgccttgag cttctccttg
     2101 tgcccgacgc cgaagcgctg ctcctcgtcg acgaccacca gccccaggtc cttgaacacc
     2161 acgtcggcgc cgaggaggcg gtgcgtgccg atgacgaggt ccacggtccc ggcggccaga
     2221 ccgtccagga tggccttctg ctccctctgg ccggagaagc gcgacagcac cgagatc
//
LOCUS       DATMKC010000180         4189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_242585, whole genome shotgun sequence.
ACCESSION   DATMKC010000180 DATMKC010000000
VERSION     DATMKC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242585"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VNU01_05280"
     CDS             <1..260
                     /locus_tag="VNU01_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32065.1"
                     /translation="AVLCSGQGEYALGRALAVRTALLASRDGAVAAAERAVEEAGPRA
                     GAETGIVVYDADADDVVARATRGTFPVLTRDRDGVRVVQVA"
     gene            complement(319..1194)
                     /locus_tag="VNU01_05285"
     CDS             complement(319..1194)
                     /locus_tag="VNU01_05285"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HWH32066.1"
                     /translation="MRSIVVVTALAVLAALAPSAVRADHAGTTIAWTALLPAQPGMPA
                     RDAASCGEDGLRCLRDVERRLARMAEEFGCDHRGVFATTYLLLTRELRVEAERRPSVF
                     EDPAAIGRLAVVFFEMYTRALADHEAGRPLPAAWRIAFDAARDGDHNAGQDMLLAISA
                     HVQHDMPFAVAVVGLRTPHGGSRKPDHDRVNQVLSRAYDRIVPAIAERYDPIMTTADA
                     KPSPADDIAAQNMVAGWREGVWRNAERLTAARHAWERAAVVHGIERNAEAWARTFAAF
                     ESPGYRATRDAYCRR"
     gene            complement(1315..2124)
                     /locus_tag="VNU01_05290"
     CDS             complement(1315..2124)
                     /locus_tag="VNU01_05290"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWH32067.1"
                     /translation="MTEPTDDAGARLIRLETEVPGTPEQVWRTIATTQGLATWYMPST
                     VEEREGGAFASDMGPWGKSSGTVTGYDPPHRFAFEETGWHPDPAAPAWATELLVEARD
                     GGTCVVRLVSGFREGGEAWEDMMGGASEGFAQALQNLRLYLTHFAGMPGFQLTAAAVA
                     RGEVEDVRARLLGNVGLAGATRGATVTVRGHTDLVGVVEEMEPSGILVRVQQPVQGLW
                     HLRTWPGAPGDVVMAIRAILYGEDAAEVAPRDERAWEAWLYEIFPDAVAPE"
     gene            complement(2121..2735)
                     /locus_tag="VNU01_05295"
     CDS             complement(2121..2735)
                     /locus_tag="VNU01_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH32068.1"
                     /translation="MQDVEVIQEPGAAVVALDPVRARLLAELRQPASAAALAERVGLT
                     RQKVNYHLRQLEAHGLVEPAGTRQWGGITERLLVASASAYLVSPAALGEASANPARTR
                     DRLSAAYLIALAARVVREVGGMARTAAREDKRLPVLALDADVRFASPEDRAAFAKELG
                     AAVTRLVAKYHDVNAPDGRWQRVLATVHPVPADVEVGPPTEETP"
     gene            2884..>4189
                     /locus_tag="VNU01_05300"
     CDS             2884..>4189
                     /locus_tag="VNU01_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32069.1"
                     /translation="MSSLLLSRRDLDFLLYEWLEVEHLSKRARFADHGRETFDAVLDL
                     AARVAEEQFAPHNKAADEHEPTFDGERVSLIPEVKQALDAFAQTGLLAAGFDYDVGGM
                     QLPQVVAMATLAWFQAANVGTGAYPFLTIGNANLLLAHGSPEQVATFVRPMVEGRWFG
                     TMCLSEPQAGSSLSDITTRAEPRGDGSYRLFGNKMWISGGDHELSENIVHLVLARIPG
                     SPPGVKGISLFVVPKYLVEADGSLGERNDVVVAGLNHKMGYRGTVNTLLNFGEGRHTP
                     GGEPGAVGYLVGEPNRGLAFMFHMMNEARVGVGGGATALGYTGYLKSLRYARERTQGR
                     PVSGKDPLAPMVPIVEHADVRRMLLAQKAYVEGALALILYCAKLVDDSKTAETQQERE
                     AAHLLLDTLTPIAKSWPSQWCLEANSLAIQIHGGYGYTREYDV"
BASE COUNT          540 a         1489 c         1576 g          584 t
ORIGIN      
        1 ttgccgtgct gtgctccggc cagggtgagt acgcgctggg gcgcgcgctg gcggtgcgca
       61 cggcgctgct ggcgtcccgt gatggcgccg tggcggcggc ggagcgggcg gtggaggagg
      121 caggtccgcg cgccggggcg gagaccggca tcgtggtcta cgacgccgac gcggacgacg
      181 tcgtggcgcg agccacgcgc gggacgttcc cggtgctcac gcgcgaccgc gacggcgtgc
      241 gcgtggtgca ggtcgcctga tctcgttgcg ggaccacttg cgtccgatag cggacacaag
      301 tcgtcccgga tcggccggtc aacggcggca gtaggcgtcg cgcgtggcgc ggtagccggg
      361 gctttcgaag gcggcgaacg tccgtgccca cgcctcggcg ttgcgctcga tgccgtggac
      421 gacggccgcg cgctcccacg cgtggcgggc ggcggtcagg cgctccgcgt tgcgccacac
      481 gccttcccgc cagccggcga ccatgttctg ggcggcgatg tcgtcggccg ggctgggctt
      541 ggcgtccgcc gtggtcatga tcgggtcgta ccgctcggcg atggccggga cgatgcggtc
      601 gtacgcccgg ctcagcacct ggttcacccg gtcgtggtcg ggcttgcgcg acccgccgtg
      661 cggtgtgcgc aggcccacga ccgcgaccgc gaacggcatg tcgtgctgga cgtgcgcgct
      721 gatggccagg agcatgtcct ggccggcgtt gtggtcaccg tcgcgggccg cgtcgaacgc
      781 gatccgccag gcggcgggca gcgggcgccc ggcctcatgg tccgccagcg cgcgcgtgta
      841 catctcgaag aacaccacgg ccaggcgccc gatcgcggcc gggtcctcga acaccgacgg
      901 ccgccgctcg gcctcgaccc gcagctcgcg ggtgagcagc aggtacgtgg tggcgaacac
      961 gccgcggtgg tcgcagccga actcctccgc catgcgggcc agtcgccgct cgacgtcgcg
     1021 caggcagcgc agcccgtcct cgccgcacga cgcggcgtct cgcgccggca ttcccggctg
     1081 ggcgggcagc agcgcggtcc atgcgatggt ggtgccggcg tggtcggctc ggacggcgct
     1141 cggcgcgagc gcggcgagca cggccaaggc ggtgaccacg acgatgctgc gcatgggctg
     1201 cctcccggcc gggtgcggcc gatcctagag cgcgcgctca cccggcggca gtgcccgcgc
     1261 tccgggacca cttgtgtccg atagcggccg caagtggtcc cggagacggc aggctcactc
     1321 cggggcgacg gcgtccggga agatctcgta cagccacgcc tcccaagcgc gctcgtcacg
     1381 cggcgccacc tcggcggcgt cctcgccgta gaggatcgcg cggatcgcca tcaccacgtc
     1441 gcccggcgcg ccgggccacg tccgcaggtg ccacaggccc tggacgggtt gctgcacccg
     1501 gaccaggatg ccgctcggct ccatctcctc gacgacgccg acgaggtcgg tgtggccgcg
     1561 gacggtcacc gtcgcgcccc gcgtggcgcc ggcgaggccg acgttgccga gcagccgcgc
     1621 cctgacgtcc tccacttccc cgcgggcgac ggcggcggcg gtcagctgga aaccgggcat
     1681 gcccgcgaag tgcgtgaggt acagccgcag gttctgcagc gcctgggcga agccctcgct
     1741 cgcgccaccc atcatgtcct cccacgcctc gccgccctcg cggaagccgc tgaccaggcg
     1801 cacgacacag gtgccgccgt cgcgtgcctc gaccagcagc tccgtggccc aggccggcgc
     1861 cgccgggtcc gggtgccagc cggtctcctc gaaggcgaag cggtgcggcg ggtcgtagcc
     1921 ggtgaccgtg ccggagctct tgccccacgg gcccatgtcg ctggcgaacg cgccaccctc
     1981 gcgctcctcc acggtcgacg gcatgtacca ggtggccagg ccctgcgtcg tcgcgatggt
     2041 gcgccagacc tgctcgggcg tgcccggcac ctcggtctcc aggcggatca ggcgggcgcc
     2101 ggcgtcgtcg gtcggctcgg tcatggtgtc tcctcggtgg gcgggccgac ttcgacgtcg
     2161 gcgggaacgg gatggacggt ggcgagcacg cgctgccagc ggccgtcggg cgcgttgacg
     2221 tcgtggtact tggccaccag gcgcgtgacg gcggcgccca gctccttggc gaaggcggca
     2281 cggtcctccg gcgaggcgaa gcgcacgtcg gcgtcgaggg cgagcacggg caggcgcttg
     2341 tcctcgcgcg cggcggtgcg cgccatgccg ccgacctccc ggacgacgcg ggcggcgagc
     2401 gcgatcaggt aggcggccga gaggcggtcg cgggtgcgcg ccgggttggc cgacgcctcg
     2461 ccgagcgccg cgggtgatac caggtacgcc gacgcgctgg ccaccaggag gcgctccgtg
     2521 atgccgcccc actgccgggt cccggccggc tccacgaggc cgtgcgcctc gagctgcctg
     2581 aggtggtagt tgaccttctg gcgggtcagg ccgacgcgct cggccagcgc cgcggcggac
     2641 gccggctggc gcagttcggc cagcagccgc gcgcgcaccg ggtccagcgc gacgaccgcc
     2701 gcgccgggtt cctggatgac ctccacgtcc tgcatggcgt gcagtgtggc gcttgacaac
     2761 tattcctgtc aagacgtccc gagcgcgcct ccgccgacca cttgtgtccg agagcggacg
     2821 caagtcgtcc cgcaacggcg ggggcactac gctgagcgct ccctcgacgt tgcggagcgc
     2881 ctcatgtcct cattgctgct gtcccgccgt gatttggact tcctgctgta cgagtggctg
     2941 gaggtcgagc acctgagcaa gcgcgcccgt ttcgccgacc atggccgcga gaccttcgac
     3001 gccgtgctcg acctggccgc gcgcgtggcg gaggagcagt tcgcgccgca caacaaggcc
     3061 gccgacgagc acgagcccac gttcgacggc gagcgcgtgt cgctcatccc ggaggtcaag
     3121 caggccctcg acgctttcgc gcagaccggc ttgctggccg ccggcttcga ctacgacgtc
     3181 ggcggcatgc agctaccgca ggtggtggcc atggcgacgc tcgcgtggtt ccaggccgcg
     3241 aacgtcggca cgggcgccta ccccttcctg accatcggca acgcgaacct cctgctggcg
     3301 cacggctcgc cggagcaggt cgccactttc gtgcgcccga tggtcgaggg ccgctggttc
     3361 ggcacgatgt gcctgtcgga gccgcaggcc ggctcgtcgc tgtcggacat caccacgcgc
     3421 gccgagccgc gcggggatgg cagctaccgg ctgttcggca acaagatgtg gatctccggc
     3481 ggcgatcacg agctgagcga gaacatcgtg cacctggtgc tggcgcgcat ccccggctcg
     3541 ccgccgggcg tgaagggcat cagcctgttc gtggtgccca agtacctggt ggaggcggac
     3601 ggctcgctgg gggagcgcaa cgacgtggtc gtggcggggc tcaaccacaa gatgggctat
     3661 cgcggcacgg tgaacacgct gctgaacttc ggcgagggcc ggcacacgcc gggcggggag
     3721 ccgggcgcgg tcggttacct cgtcggcgag cccaacaggg gcctggcgtt catgttccac
     3781 atgatgaacg aggcgcgcgt cggcgtcggc ggcggcgcga cggccctggg ttacacgggc
     3841 tacctcaagt cgctcaggta cgcgcgggag cgcacgcagg gccggccggt gtcgggcaag
     3901 gaccccctcg cgccgatggt gccgatcgtc gagcacgccg acgtgcgccg catgctgctg
     3961 gcgcagaagg cctacgtcga gggcgcgctg gcgctgatcc tgtactgcgc caagttggtg
     4021 gacgactcca agacggccga gacgcagcag gagcgcgagg ccgcgcacct gctgctggac
     4081 acgctgacgc cgatcgccaa gagctggccg tcgcagtggt gcctggaggc gaactcgctg
     4141 gcgatccaga tccacggcgg ctacggctac acgcgcgagt acgacgtcg
//
LOCUS       DATMKC010000181         3175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_292638, whole genome shotgun sequence.
ACCESSION   DATMKC010000181 DATMKC010000000
VERSION     DATMKC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292638"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1684)
                     /locus_tag="VNU01_05305"
     CDS             complement(38..1684)
                     /locus_tag="VNU01_05305"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWH32070.1"
                     /translation="MNTSTRDLAARREAVRVAPPPLAVAQAEAGPDGLLVVSPDGRML
                     FTNGRFAEIWGFDRELIAEGDDGRALEEAVSRVVDPERFLARVREIYQRPARSHDEIR
                     LTDGRVLDRYGAPLHAPDGEYLGYAWYFRDVTAQKQTESQLRELVRTLQASLLPPRPP
                     AIPGMEVAVRHRPGSPGLSVGGDFYDVFRLRGDAWGVAIGDVCGKGAQAAALTALARY
                     SLRGAAMHAESPAAVLGEVNETLLEDQTLGERFCALVYARVEHDWCGAWVTLAAAGHP
                     LPLVLRSSGWVDVRGQHGGLLGLFDDVELSEDRVGLGPGDAIVLCTDGVSEARDARGE
                     LFGDERLFAVLSAHAGQPAERIASEVVDQAVAFSAGPQCDDIAVVVLRVPPQAAEDPE
                     GRLRQAIGRVPARRSSSCGGTRGGARVTPPREARLPLRAEAAAAREARAFVRAVLRSW
                     RIHDVGMTDAELVAAELVANAIRHTGQPRVMVVAYDGGAVRLEVGDGSRLAPRPRLAG
                     HDATDGRGLSIVAALASRWGVDLVPGGKRVWAELPTSGSD"
     gene            complement(1852..>3175)
                     /locus_tag="VNU01_05310"
     CDS             complement(1852..>3175)
                     /locus_tag="VNU01_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32071.1"
                     /translation="SVIGVQGTSFAAPYVSGALALVRAERGDLSPASVLDLLASTAKD
                     LGVPGRDREYGHGLVQPRAMLVAAAGVAQPAPVVSEPVDGGGTAQPEPAPEPDADPAP
                     AVARVSGGDGTTEAVAQAVAVSRLAFPRNGARHAVLARGDDFADALAGSALGMGAGPL
                     LFAPREGPLPDATRTELARVLPDGAVVYLLGGEAALSAGLDDELAAVGLRPVRLAGAS
                     REETAAVVAREVVRRAGELGKPGAGRVLLATRGDWPDAVSGGGLAALLAAPVLLTAPD
                     ALHPATARALEELTPGRVLVVGGTAAVSDAVAQEAGAAAGARVQRLSGPARDATAVAV
                     AQEAHRLLGVRGQAPRYAVAVNLRRADAFAHALSASVLSGATGALLVPVERDAGTELT
                     QAARDWAREALHDPATDVLLAGEADIVADQVGEELGRLLAQARAARQA"
BASE COUNT          379 a         1285 c         1202 g          309 t
ORIGIN      
        1 gccgtccgcg gcggtcaacg gcaggggcgc aaaggggtca atccgatccc gaggtgggca
       61 gttccgccca gacgcgcttg ccgccgggca ccagatccac gccccaccgg ctggccagcg
      121 ccgcgacgat ggacagtccg cgtccgtccg tggcgtcgtg gccggccaga cgcggccggg
      181 gcgccagccg cgagccatcc ccgacctcga ggcggacggc gccaccgtcg taggccacca
      241 ccatgacgcg cggctggccg gtgtggcgga tagcgttcgc gaccagctcc gcggccacca
      301 gctccgcgtc ggtcatcccg acgtcgtgga tccgccagct gcgcaggacg gcgcgcacga
      361 aggcgcgggc ctcgcgcgcc gcggccgcct ccgcgcgcag cggcagccgc gcttcgcgag
      421 ggggcgtgac ccgggccccg ccgcgggtac cgccgcacga ggaggaccgg cgcgcgggca
      481 cccggccgat ggcctggcgg agccgcccct ccgggtcctc ggccgcctgc ggggggaccc
      541 gcagcaccac cacggcgatg tcgtcgcact gcggtccggc ggagaacgcc accgcctggt
      601 ccacgacctc cgacgcgatg cgctccgccg gctggccggc gtgcgcggac aggacggcga
      661 acagccgctc gtccccgaac agctccccgc gcgcgtcgcg cgcctcgctc accccgtcgg
      721 tgcacagcac gatcgcgtca ccgggcccca gcccgacacg gtcctcgctc agctccacgt
      781 cgtcgaacag gccgagcagg cccccgtgct ggccgcgcac gtccacccag ccggagctgc
      841 gcagcaccag gggcagggga tgccccgcgg cggccagcgt cacccaggcg ccgcaccagt
      901 cgtgctcgac gcgcgcgtac accagcgcgc agaagcgctc ccccagggtc tggtcctcca
      961 gcagcgtctc gttgacctcg ccgagcaccg ccgccgggga ctcggcgtgc atggcggcgc
     1021 cgcgcagcga gtagcgcgcc agcgcggtga gggcggccgc ctgggcgccc ttgccgcaga
     1081 cgtcgccgat ggccacgccc caggcgtcgc cgcgcagccg gaacacgtcg tagaagtccc
     1141 cgcccaccga cagcccggga ctgccggggc ggtgacgcac cgcgacctcc atgccgggga
     1201 ttgcgggcgg gcggggcggc agcaggctgg cctgcagcgt gcgcacgagt tcgcgcagct
     1261 gggactcggt ctgcttctgc gcggtcacgt cgcggaagta ccaggcgtag cccaggtact
     1321 ccccgtccgg cgcgtgcagc ggcgcgccgt agcggtccag cacgcggccg tcggtgaggc
     1381 ggatctcgtc gtggctgcgc gcggggcgct ggtagatctc gcgcacgcgc gccaggaagc
     1441 gctccgggtc caccacccgc gagaccgcct cctccagcgc gcggccgtcg tccccctccg
     1501 cgatcagctc gcggtcgaag ccccagatct ccgcgaagcg gccgttcgtg aacagcatgc
     1561 gcccgtccgg cgacaccacc agcaggccgt ccggcccggc ctcggcctgg gccaccgcca
     1621 gtggcggcgg cgcgacgcga acggcttccc gccgcgccgc gaggtctcga gtggatgtgt
     1681 tcacgagcgc cttcccgccg tgcggcgcgc gccgcctgtc cttcccgcac gcgagcccgg
     1741 ggagcgccgc cgcgcgggcg cggcggcagc ggccgtgtgc ggaccgtcga cagggggtac
     1801 ccggcaccgc gtgccgtgaa acgccggagc agcgggcagg cgcctgccgt gtcaggcctg
     1861 ccgggcggcg cgcgcctgcg ccagcagccg gcccagttcc tcgccgacct ggtcggcgac
     1921 gatgtcggcc tcgccggcca gcagcacgtc ggtggccggg tcgtgcagcg cctcccgggc
     1981 ccagtcgcgc gcggcctgcg tcagctccgt gccggcgtcg cgctccaccg ggacgagcag
     2041 cgcgccggtg gcgccggaca gcacggaggc ggacagggcg tgcgcgaagg cgtcggcgcg
     2101 ccgcaggttc accgccaccg cgtagcgcgg cgcctggccg cgcacgccga gcaggcgatg
     2161 cgcctcctgc gccacggcca cggccgtggc gtcgcgcgcc gggccggaca gccgctggac
     2221 gcgggcgccg gcggcggcgc cggcctcctg ggcgacggcg tccgacacgg cggcggtgcc
     2281 accgaccacg agcacgcggc cgggcgtgag ctcctccagc gcccgagccg tggcggggtg
     2341 cagcgcgtcc ggcgcggtga gcagcaccgg cgcggcgagc agggccgcca gcccgccgcc
     2401 gctgacggcg tccggccagt cgccgcgcgt ggccagcagg acgcggccag cgccgggctt
     2461 gcccagttcg ccggcgcggc ggaccacctc gcgggcgacg accgcggcgg tctcctcgcg
     2521 tgaggcgcca gccaggcgca ccggccgcaa gcccaccgcc gccagctcgt cgtcgagccc
     2581 ggcggacagc gcggcctcgc caccgagcag gtagacgacc gcgccgtccg gcaggacgcg
     2641 cgccagctcg gtccgcgtcg cgtcgggcag cgggccctcg cggggggcga acagcagcgg
     2701 cccggcgccc atccccagcg cgctgccggc cagcgcgtcg gcgaagtcgt cgccgcgcgc
     2761 gagcacggcg tgccgcgctc cgttgcgggg gaacgccagg cgcgacaccg cgacggcctg
     2821 ggccacggcc tccgtggtgc cgtcgccgcc ggacacgcgc gccacagccg gggccgggtc
     2881 cgcgtcgggc tcgggcgccg gctccggctg ggcggtcccg ccgccgtcca ccggctcgga
     2941 gaccaccggg gccggctgcg ccacgcccgc ggcggccacc agcatcgccc gcggctgcac
     3001 cagcccgtgg ccgtactcgc ggtcacggcc cggcacgccc aggtccttcg cggtggaggc
     3061 cagcaggtcc agcacggacg ccggcgacag gtcgccgcgc tcggcgcgca ccagggcgag
     3121 cgcgccactg acgtaaggcg cggcgaacga ggtgccctgc acgccgatca ccgac
//
LOCUS       DATMKC010000182         3327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_1353, whole genome shotgun sequence.
ACCESSION   DATMKC010000182 DATMKC010000000
VERSION     DATMKC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VNU01_05315"
     CDS             complement(<1..518)
                     /locus_tag="VNU01_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWH32072.1"
                     /translation="MQLADGAEHPPAGQRIARSVARQAPSHHNEETTMKHSLSAAAAG
                     LLALGLAPAHAQGGAPASGVTIYGVADAGVEWSNNGAGSSRRLVSGGALGSRLGFRGV
                     EDLGGGLSAVFRLEQGINLDDGTLGQGGRAFGREASVGLASTAWGTVTAGRLPTPYYV
                     AQSGVDAFSWMG"
     gene            complement(586..1545)
                     /locus_tag="VNU01_05320"
     CDS             complement(586..1545)
                     /locus_tag="VNU01_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWH32073.1"
                     /translation="MAAALLGSCIALCAHANGYPSRPVTLVVPFTPGGSNDALARTVG
                     QKLGEYWKQPVIVDNRPGAGGNVGTKQAAKAAPDGHTLLVVANNFVTNPFLYPDGRAG
                     YDPVKEFVPVTQLGRVPFVLVVHPGLAAKSTQELIALAKAQPGKLSYGSAGVGTPHHL
                     TGELFKSLAGIDMVHVPYRGAQPVVTDLIGGQIQVLFGVANSVLPHIKTGALRPLAVA
                     GEQPLPYLPNVPTVASAGGPFKAFRSEVWIGLVAPAGTPPDVVAKIEEAAGRALRDPA
                     VKAALEAQGLEPAPSTPAALQALMQEDMARWSKVIKDTGARAE"
     gene            complement(1621..3237)
                     /locus_tag="VNU01_05325"
     CDS             complement(1621..3237)
                     /locus_tag="VNU01_05325"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH32074.1"
                     /translation="MGTEIKTPVLIVGGGPIGLSMAIELGWRGIESILVDEGDGTIEH
                     PRTGLIAVRTMELFRRWGLAQRVRDCGFPDDYDLSMVFCTSLNGLLLDRESYPSMRDA
                     PTPPETPEKKQRCPQLWLQPILTDAARAEPRARLLFKHRFVSLQQDGEGVTATVTDLN
                     TGEPVTIRADYLLGCDGATSQVREQVGIRMEGRLLSYSVNVLIHAPGLVHEHKMGPAE
                     RYLFVGPEGTWGNLTVVDGNEIWRLTVLGSEEKMDLANFDPAAWVRRALGRDDVEFVV
                     DSAIPWRRSEMLADRYFEGRVALVGDSAHTMSPTGGMGMNTGAQEVLDIGWKLEGLLN
                     GWGGPALLRSYETERRPVAQRNIGFSTQNFRAWQDTPSPAAVCDLSAEGEQVRRDLGR
                     RLRESTRVEWESLGLQIGHRYEGSPICVSDGTPPTPDEYSTYIPTTRPGARAPHVWLS
                     DGRSTLDLFGRTFVLLCFDGGLRGEADTLAQAFAARGVPFRLESIEQADVARAYERPL
                     VLVRPDGHVAWRGSHVEHAGQVVDTVRGAA"
BASE COUNT          461 a         1143 c         1207 g          516 t
ORIGIN      
        1 ctgcccatcc acgagaaggc gtcgacgccg gactgggcca cgtagtaggg cgtcggcagg
       61 cgcccggccg tcacggtgcc ccaggcggtg cttgccaggc cgacggaggc ttcccggccg
      121 aatgcgcggc cgccctggcc cagggtgcca tcgtccagat tgatgccttg ctccaggcga
      181 aaaaccgcgg acaggccgcc gccgaggtcc tcgacgccgc ggaagccgag ccggctgccc
      241 agcgcgccgc ccgacaccag ccggcgggac gagccggcgc cgttgttaga ccattcgacc
      301 ccggcgtcgg ccacgccgta gatcgtgacg ccggacgccg gcgcgcctcc ttgcgcatgc
      361 gcgggtgcga gcccgagcgc gagcaggcct gcggccgccg cggaaaggga atgcttcatg
      421 gttgtctcct cgttgtgatg ggatggggcc tgacgggcca cgctgcgggc gatgcgctgc
      481 ccggcgggcg ggtgctccgc gccgtcagcc agctgcaatc gggtgttaga gggcggcgcg
      541 gcggcgccgg cggcggcgat cagccgccgg cctcgccgct gcgcctcact cggcgcgtgc
      601 gccggtgtcc ttgatgacct tggaccagcg cgccatgtct tcctgcatga gtgcctggag
      661 cgcggccggc gtcgatgggg cgggctccag gccctgggct tccagcgccg ccttgaccgc
      721 cggatcccgc agtgcccggc ccgccgcctc ctcgatcttg gccaccacgt cgggcggcgt
      781 gcccgccggc gcgaccaggc cgatccagac ctcgctgcgg aaggccttga aggggccgcc
      841 cgcgctggcg acggtcggga cgtttggcag gtagggcagg ggctgctcgc cggccaccgc
      901 gagcgggcgc agggcgccgg tcttgatgtg cgggagcacc gagttcgcga cgccgaagag
      961 cacctggatc tgtccgccga tcaggtccgt gaccacgggc tgcgcgccgc ggtagggcac
     1021 gtgcaccatg tcgatgccgg ccaggctctt gaagagctct ccggtcaggt ggtgcggcgt
     1081 gccgacgccg gcggacccat aggacagctt gccgggctgg gccttggcca gggcgatcag
     1141 ctcctgggtc gacttggcgg cgaggccggg gtggacgacc aggacgaagg gcacgcggcc
     1201 cagctgcgtg acgggaacga attccttcac gggatcgtag cccgcgcggc cgtccggata
     1261 gaggaagggg ttggtgacga aattgttggc cacgacgagc agggtgtggc cgtcgggagc
     1321 cgccttggcc gcctgcttgg tgccaacgtt gccgcccgcg cccgggcggt tgtcgacgat
     1381 gacgggctgc ttccagtact cgccgagctt ctggcccacc gtgcgcgcca gggcgtcgtt
     1441 gctgccgccg ggcgtgaagg gcacgaccag cgtgaccggc ctgctgggat agccgttggc
     1501 atgtgcgcac agtgcgatgc acgagccgag aagggcggcg gccatcgcct tgatcgcgcg
     1561 gccgggcagt tcagtcttca tggggttgtc tcctttttgt ttcgtggaag gggcttgcgg
     1621 ctaagcggcg ccgcggaccg tgtcgaccac ctggccggcg tgctccacgt gcgagccgcg
     1681 ccatgcgaca tgcccgtcgg gacgcaccag gaccagcggg cgttcgtagg ctctcgccac
     1741 gtcggcctgc tcgatggatt cgaggcggaa cggaactccc ctggcggcaa aggcctgcgc
     1801 gagcgtgtcc gcctcgcccc gcaggccgcc gtcgaagcac aggagcacga aggtgcggcc
     1861 gaacaggtcg agcgtcgagc ggccgtcgct gagccagacg tggggcgcgc gcgcgccggg
     1921 ccgcgtcgtg gggatgtacg tcgagtactc gtccggggtc gggggcgtgc cgtcggaaac
     1981 gcagatcggc gagccctcgt agcggtggcc gatctgcagg cccagcgact cccactccac
     2041 gcgcgtcgac tcgcgcaggc gccggccgag gtcccggcgc acctgctcgc cttcggcgct
     2101 caggtcgcac accgccgccg ggctgggcgt gtcctgccag gccctgaagt tctgcgtgga
     2161 gaagccgatg ttgcgctgcg cgacgggccg gcgctcggtc tcgtagctgc gcagcagggc
     2221 cgggccgccc cagccgttga ggaggccctc gagcttccag ccgatgtcca gcacctcctg
     2281 cgccccggtg ttcatgccca tgccaccggt cggagacatg gtgtgggcgg aatcgcccac
     2341 gagcgcgacg cggccctcga agtagcgatc ggcgagcatc tcgctgcgcc gccaggggat
     2401 ggccgaatcg accacgaact ccacgtcgtc gcgaccgagc gcgcgccgga cccaggcggc
     2461 ggggtcgaag ttcgcgaggt ccatcttctc ttccgatccg agcaccgtca ggcgccagat
     2521 ctcgttcccg tcgacgacgg tcagattgcc ccacgttccc tccgggccga cgaagaggta
     2581 gcgctcggcc ggccccatct tgtgctcgtg caccagtccc ggcgcgtgga tcaggacgtt
     2641 caccgagtag ctgagcagcc ggccctccat cctgatgccg acctgctcgc ggacctggct
     2701 ggtagcgcca tcgcagccga gcaggtagtc cgcgcggatg gtgacaggct cgcccgtgtt
     2761 caggtcggtg acggtcgccg tgacgccctc gccgtcctgc tgaagcgaga cgaagcggtg
     2821 cttgaacagc agcctggccc tgggttcggc gcgggcggcg tcggtcagga tgggctgcag
     2881 ccacagctgc gggcagcgct gcttcttctc gggcgtctcg ggcggcgtgg gcgcgtcgcg
     2941 catgctcggg tagctctccc gatcgagcag cagcccgttg agcgacgtgc agaagaccat
     3001 cgagaggtcg tagtcgtccg ggaatccgca gtcgcgcacg cgctgcgcca gcccccagcg
     3061 tcggaacagc tccatcgtgc gcaccgcgat caggccggtg cgcgggtgct cgatcgtgcc
     3121 gtcgccctca tccacgagga tcgactcgat ccccctccat cccagctcga tggccatcga
     3181 caggccgatg ggccctccgc ccacgatcag cacgggcgtc ttgatctctg tccccatggg
     3241 gtctcatcct ttctcgtttg gttggggaaa tgatcaccgg cacgctcatc attgtctaat
     3301 cgatattgga gagaaataga attcagg
//
LOCUS       DATMKC010000183         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_99871, whole genome shotgun sequence.
ACCESSION   DATMKC010000183 DATMKC010000000
VERSION     DATMKC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99871"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..880
                     /locus_tag="VNU01_05330"
     CDS             119..880
                     /locus_tag="VNU01_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH32075.1"
                     /translation="MTAADDAPIVVLLHGVGVGAESFQPVARALVPGHGVALPDRSGY
                     GTGAGAPRTRIEDHVEDFGRFLTSLPRPPAVVAGVSGGATIALAAALAGALAGRDGSP
                     PGLLLHEPLLGELVPELHAAASAAADALQRGEPGGENAFLRRLAGEPLPPVGPEVGAV
                     VRAEVPSFLAFQPDIRDLERLGELPLLVTTIGDRSGRPRQAAAELLAQAGGAHVVTIP
                     GCGHLPQLQAPERLAAAIAATGRGAAARRASVCLS"
     gene            868..1473
                     /locus_tag="VNU01_05335"
     CDS             868..1473
                     /locus_tag="VNU01_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWH32076.1"
                     /translation="MPVLTGAERAVGRVPGSDPYPWPYDGDLEPARMALVVAGASSAW
                     RSRCPDLAIAERNIGLLSGAMRSAGAVVVTVHQGVPARAGFFGEEGEPLVLRGARRVH
                     AAGLDAFYGGPLEPALRALGRDLLVLAGYGLEGPVHSTLRGANDRGLECLLVTDACTS
                     TDQGLRDAAARTVTMSGGIFGAVGDTASVCAALEARKDALR"
     gene            1470..2183
                     /locus_tag="VNU01_05340"
     CDS             1470..2183
                     /locus_tag="VNU01_05340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWH32077.1"
                     /translation="MTAELTVDRSFPAVASEPYPWPYDSRIDPARTALVMVDWQTDFC
                     GPGGYVDAMGYDLDLTRAGLAPAARVLAAAREAGLTVVHTREGHKPDLSDCPPNKLWR
                     SRRIGAGIGESGPCGRILVRGEPGWEIVPEVAPLTGEVIIDKPGKGSFYATDLDLILR
                     NRGITHLVLTGITTDVCVHTTMREANDRGFECLLLTDCTGATDVGNYEAALKMVTMQG
                     GVFGAIAPSDALLAALASA"
     gene            2180..>2692
                     /locus_tag="VNU01_05345"
     CDS             2180..>2692
                     /locus_tag="VNU01_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWH32078.1"
                     /translation="MTAALQRRSVRALLDDYRAGRTTPAEVLARTRERFLAGDPAIWI
                     SVAGQDALDRRAAQLSAVPDGPSLLPLYGIPVAVKDNIDVAGMVTTAACPSFAYRAAH
                     SATAVRLLEAAGAVVVGKTNLDQFATGLVGTRSPYGVPRNPAAPAHIPGGSSSGSAVA
                     VASGQVPLALG"
BASE COUNT          331 a         1015 c          993 g          353 t
ORIGIN      
        1 tcgccggacc ggtcgtcgac caggacggca ccgagcgggt cgccgccggg cagcagccca
       61 cgtacgagga ggtcgagacc atggactact tcgtccaggg tgtgatcggt tcgctgcaat
      121 gacggctgcc gacgacgcgc cgatcgtcgt gctgctccac ggcgtgggcg tcggcgcgga
      181 gtcgttccag cccgtcgccc gggccctggt ccccggacac ggcgtggccc tgccggaccg
      241 ctccgggtac ggcacgggcg cgggcgcgcc gcgcacccgg atcgaggacc acgtcgagga
      301 cttcggccgg ttcctcacga gcctcccccg gcccccggcc gtggtggccg gtgtgagcgg
      361 cggggccacg atcgccctgg ccgccgcgct ggccggcgcg ctcgcaggcc gggacggctc
      421 cccgccgggc ctcctgctgc acgagccgct gctcggcgag ctggtgcccg aactgcacgc
      481 cgcggcctcg gcggccgcgg acgcactgca gcgcggcgaa cccggcggtg agaacgcctt
      541 cctgagacgg ctcgcgggcg agcctctgcc gcccgtgggg ccggaggtgg gagccgtggt
      601 gcgtgccgag gtgccgagct tcctggcttt ccagccggac atccgcgacc tggagcggct
      661 cggcgagctg cccttgctgg tcaccaccat cggggaccgc agcggccgcc cgcggcaggc
      721 cgccgccgag ttgctcgcgc aggccggcgg tgcccacgtc gtcaccatcc cgggctgcgg
      781 gcacctgccg cagcttcagg cgccggagcg cctcgccgcc gcgatcgcgg ccacgggccg
      841 cggtgcggcc gcgcggaggg cgagcgtatg cctgtcctga caggagccga gcgagccgtc
      901 ggccgcgtgc ccggcagcga cccctatcca tggccgtacg acggcgacct ggagcccgcg
      961 cgcatggcac ttgtggtcgc gggagcgagc tccgcgtggc ggagccgttg cccggacctc
     1021 gccatcgcgg agcgcaacat cggcctgctg agcggcgcca tgcgcagcgc cggcgcggtg
     1081 gtggtgaccg tccatcaggg tgtgcctgcc cgcgccggct tcttcggaga ggaaggcgag
     1141 ccgctggtgc tgcgaggcgc gcggcgtgtc cacgcggccg gtctggacgc cttctacggg
     1201 gggccgcttg agccggccct gcgcgccttg ggccgcgacc tgctggtgct ggcgggatac
     1261 ggcctcgaag gcccggtgca ctcgacgctg cgcggagcca acgaccgtgg gctggagtgc
     1321 ctgctcgtca ccgacgcgtg cacctcgacc gatcaggggt tgcgcgacgc cgcggcccgc
     1381 accgtgacga tgtctggcgg catcttcggc gcggtgggtg acaccgcgtc cgtctgcgcc
     1441 gccctggaag cacgaaagga cgccctgcga tgaccgccga gctgaccgtc gaccgcagct
     1501 tccccgcggt cgcctccgag ccgtacccgt ggccgtacga cagccgcatc gacccggccc
     1561 gcaccgcctt ggtgatggtc gactggcaga ccgacttctg tggcccgggg ggatacgtcg
     1621 acgccatggg ctacgacctc gacctgacgc gcgccgggct ggcgccggcg gcacgggtcc
     1681 tggccgcggc gcgggaggcc ggcctcacgg tcgtccacac gcgcgagggc cacaagccgg
     1741 acctgtcgga ctgcccgccg aacaagctct ggcgctcccg ccgcatcggc gccggcatcg
     1801 gcgagagcgg gccgtgcggc cgcatcctcg tgcgcggcga accgggttgg gagatcgtgc
     1861 cggaggtcgc gccgctgacg ggcgaggtga tcatcgacaa gccgggaaag ggctccttct
     1921 acgcgaccga cctggacctc atcctgcgca accgcggcat cacccacctc gtgctcaccg
     1981 gcatcacaac cgacgtgtgc gtccacacca ccatgcggga ggcgaacgac cgcggcttcg
     2041 agtgcctcct gctgaccgac tgcaccggcg cgaccgacgt gggcaactac gaggccgcgc
     2101 tgaagatggt gacgatgcag ggcggcgtct tcggcgcgat cgcgcccagc gacgcgctgc
     2161 tggcggcgct cgcctcggcg tgaccgcggc gctgcagcgg cgatccgtcc gcgcgctgct
     2221 cgacgactac cgtgccggcc ggaccacgcc cgccgaggtc ctcgcgcgca cgcgcgagcg
     2281 gttcctggca ggcgaccccg cgatctggat cagcgtcgcg ggtcaggacg cgctcgatcg
     2341 gcgcgcggcc caactgagcg ccgttccaga cggtccgtcg ctcctgccgc tctacggcat
     2401 cccggtcgcc gtgaaggaca acatcgatgt cgccggcatg gtgaccacgg ccgcctgccc
     2461 gtccttcgcc taccgggcgg cgcactcggc caccgcggtc cgcctcctcg aggcggcggg
     2521 cgccgtcgtg gtcggcaaga ccaatctgga ccagttcgcc acagggctcg tgggcacgcg
     2581 ttcgccctac ggggtcccgc gcaacccggc ggccccggcg cacatccccg gcggatccag
     2641 ctccggctcg gccgtcgcgg tggcgagcgg acaggtgccg ctcgcgctcg gc
//
LOCUS       DATMKC010000184         5510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_164129, whole genome shotgun sequence.
ACCESSION   DATMKC010000184 DATMKC010000000
VERSION     DATMKC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164129"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VNU01_05350"
     CDS             complement(<1..384)
                     /locus_tag="VNU01_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32079.1"
                     /translation="MAADGGPEERAHGLRLVATGAAVVVLIGLAGVTVREAARLAPGT
                     TGDPEVLAGSDAGTSEPSPPPAPGSWELLPRAPIASRADALALWTGSEVFVWGGWSES
                     GGRRSALADGALYEPARPGWRARWSP"
     gene            complement(390..638)
                     /locus_tag="VNU01_05355"
     CDS             complement(390..638)
                     /locus_tag="VNU01_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWH32080.1"
                     /translation="MSAEPLASQPPVVDSLGKACPIPIIDLARAITDVPVGAELVVLA
                     DDPGAKVDIPVWCRMKGQEFLGMADAERGWSFTVRRVR"
     gene            complement(648..1730)
                     /locus_tag="VNU01_05360"
     CDS             complement(648..1730)
                     /locus_tag="VNU01_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWH32081.1"
                     /translation="MAAAPARVYLDHASATPWHPAALAAARQVAESLPGDPARRHREG
                     RAAGLLQQIAGGAPLAVAANRSRPARILSSAVEHSAVLAAADAAAEASGGQHVRVPVD
                     AVGRVDLDALQAELRGGALLVNLLHAGHEVGTLQPLAEAAALCAEHDALLHVDACQTA
                     GRLPVSLEGIGADFMSVSAAKFGGGRGTGALLWSPRARLRPLLRGDERERRVRAGLEH
                     LPGVAAMAEAVLQLRPSDPHGPAAGEAARQDALRRRLRAGVPEAVDDVVVHGPDDATA
                     PHIVALSALYCDGETMVSELDARGFAVHSGSSCATTSGEPSHVLVAMGALTHGHVRVS
                     LGPGVEAAHTDAFTGALADVVRTLRG"
     gene            1894..3756
                     /locus_tag="VNU01_05365"
     CDS             1894..3756
                     /locus_tag="VNU01_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWH32082.1"
                     /translation="MGTKTVQRVATVTIRFAGDSGDGMQLTGDRFTSVTAQLGNDLAT
                     LPDFPAEIRAPAGSLPGVSGFQLHFADHDILTPGDQPDVLVAMNPAALRTNIGDLQPG
                     GTLIVNSDAFTAGNLKKAKYAADPLRDGSLEGYQVFEVPMTTMTMGALESAELGKKDK
                     ERAKNMFALGLVSWMYSRPLESTEQWLRQKFAKRPEIAEANVAVLHAGWAFGETTEIF
                     SHSYEVQPAALPTGRYRQITGNTALAYGLIAASERSGLPIFLGSYPITPASDILHELS
                     RHKEFGVRTMQAEDEIAGIGAALGASFAGMLGVTTTSGPGVALKSETIGLAVSVELPL
                     VIVDIQRGGPSTGLPTKTEQADLLQAMYGRNGEAPVPIVAPATPGDCFDAAIEAARIA
                     LKYRTPVFLLSDGYLANGAEPWRVPDPDGLPDLREVVNFATVPNHDDGFWPFQRDPET
                     LARPWAVPGTPGLEHRIGGLEKADGSGNISYDPDNHHRMVELRAAKVAGIAKDIPPLE
                     VDDPDGHARVLVIGWGSTYGPIKAACRRARSRGTPVAQAHLRHLNPFPANAGEVLARY
                     DRVLVPEMNLGQLRMLLRAQFLVDAVGYNRVRGLPFTSSELEEAIVGLEQEGAR"
     gene            3753..4760
                     /locus_tag="VNU01_05370"
     CDS             3753..4760
                     /locus_tag="VNU01_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWH32083.1"
                     /translation="MTTALPLTKKDFVTDQEVRWCPGCGDYAVLATVQGVLPELGIAR
                     ENLVFVSGIGCSSRFPYYVDTYGFHSIHGRAPAIATGVAASRPDLKVFVVTGDGDGLS
                     IGGNHLIHALRRNNNLTIILFNNQIYGLTKGQYSPTSELGKVTKSTPFGSVDQPFNPV
                     SVALGAEATFVARSIDNDREHLGRMVKLAAQHKGASLIEVYQNCNIFNDGAFDAVRDH
                     KANQIRLEDGQPIRFGQDGEMGVVMEHDGTLRMAPVAEVGEERVLVHDAHRDDPGLAF
                     ALSRLSSGPTGPTPIGVFRQVSRPVYGLEMQRQIDDATARRGRGDLAALLAGTDTWDV
                     P"
     gene            4787..5275
                     /locus_tag="VNU01_05375"
     CDS             4787..5275
                     /locus_tag="VNU01_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32084.1"
                     /translation="MKVLLVTTDARIEADVRAALMGDDVHVLQVATPQRALAYVLERQ
                     GELDIVVADGDTAPTGGFAFSHQLKQRKDMGDDVPPIVLLIARDDDRWMAKWSRADAY
                     IRKPIDPFDLEQVVRAIVAGEAIPERPRVHAAPGEFEAILAASKVLAGGAAASGPISA
                     GP"
     gene            5286..>5510
                     /locus_tag="VNU01_05380"
     CDS             5286..>5510
                     /locus_tag="VNU01_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32085.1"
                     /translation="MTTAGGTQGAAPSWPDLLVRIQRREDLDDDTAAAAMTAIMEGQV
                     TPLQVAALLMGLRVKGETAEEVAGFVRTMRR"
BASE COUNT          778 a         2074 c         1996 g          662 t
ORIGIN      
        1 aggactccac cgggcgcgcc atcctggccg ggccggttcg tacagcgcgc cgtcggcaag
       61 cgcgcttcgc cgcccgccgc tctccgacca ccctccccac acgaacacct cgctgccggt
      121 ccagagtgcg agcgcgtcgg cccgggacgc gatcggcgcg cgaggcagca gctcccatga
      181 gcctggcgcg ggcggcggtg acggctcgct cgtcccggcg tcggagccgg cgaggacctc
      241 cgggtcgccc gtggtgccgg gagccagtcg cgccgcctcg cgcacggtca cccccgccag
      301 gccgatcaac acgacgaccg ccgcgccggt ggcgaccaga cgcaggccgt gcgcgcgctc
      361 ctccggcccc ccgtcggccg ccatggcgct cagcgcaccc tgcgcaccgt gaacgaccag
      421 ccgcgctcgg cgtcggccat gcccaggaac tcctggccct tcatgcggca ccacaccggg
      481 atgtccacct tggcgccggg gtcgtcggcc aggaccacca gctccgcgcc gacgggcacg
      541 tccgtgatcg ccctggccag gtcgatgatc gggatggggc aggccttgcc caggctgtcg
      601 accacgggcg gctgggaggc gaggggctcg gcgctcatct ggccagccta gccccgcagg
      661 gtgcgcacga cgtcggcgag cgcgcccgtg aacgcgtcgg tgtgcgccgc ctccacaccg
      721 gggccgagcg agacgcggac gtgcccgtgc gtcagcgcgc ccatggccac cagcacgtgc
      781 gacggctcgc cggacgtcgt cgcgcacgac gagccggagt gcaccgcgaa gccccgggcg
      841 tcaagctcgc tgaccatcgt ctcgccgtcg cagtacagcg cggacagcgc cacgatgtgc
      901 ggcgcggtcg cgtcgtccgg accgtgcacc accacgtcgt ccaccgcctc gggcacgccc
      961 gcccgcagcc gccgtcgcag cgcgtcctgc cgcgccgcct cgccggccgc cgggccgtgc
     1021 gggtccgacg ggcgcagctg cagcacggcc tcggccatcg ccgccacgcc gggcaggtgc
     1081 tccaggcccg cgcgcacgcg ccgttcgcgc tcgtcgccgc gcagcagcgg gcgcagccgc
     1141 gcgcggggag accacagcag cgcgcccgtg ccacggccgc cgccgaactt cgcggcggac
     1201 acggacatga agtcggcgcc gatgccctcc agggacacgg gcaggcggcc cgcggtctgg
     1261 caggcgtcca cgtgcagcag cgcgtcgtgc tcggcgcaca gcgccgccgc ctcggccagc
     1321 ggctgcagcg tgccgacctc gtggccggcg tgcagcaggt tgaccagcag cgcgccgccg
     1381 cgcagctcgg cctgcagggc gtcgaggtcc acccggccca ccgcgtcgac cggcacgcgc
     1441 acgtgctggc cgccggaggc ctccgccgcg gcgtccgcgg ccgccagcac cgcggagtgc
     1501 tccacggcgc tggacaggat ccgcgccggc cgtgaccggt tcgccgccac cgcgaggggc
     1561 gcgccgcccg cgatctgctg gagcaggccc gcggcgcgcc cctcgcggtg gcggcgggcc
     1621 gggtcgccgg gcagcgactc ggcgacctgg cgggcggccg ccagggccgc cgggtgccac
     1681 ggcgtggcgg acgcgtggtc gaggtagacg cgggctgggg cggcggccat gcgacgcagt
     1741 atcgcgggtt gccgcgcatc ggcgggggcc ggtgactaca gtggggttgt gcgctcgccg
     1801 tgaggcgacc gccggaccgt tcccccatgc acaccccggc gcgcgggcgc cggagtccga
     1861 cagaccattc ggcaacggcg ccggaggcaa gcgatgggaa ccaagaccgt tcagcgcgtc
     1921 gcgaccgtga cgatccgctt cgcgggcgac tcgggtgacg gcatgcagct caccggcgac
     1981 cgcttcacgt ccgtgaccgc gcagctgggc aacgacctgg ccacactgcc ggatttcccc
     2041 gccgagatcc gcgcgcccgc gggctcgctg ccgggcgtgt ccggcttcca gctgcacttc
     2101 gccgaccacg acatcctcac gcccggcgac cagcccgacg tgctcgtggc gatgaacccg
     2161 gcggcgctgc gcaccaacat cggcgacctg cagccgggcg gcacgctgat cgtcaacagc
     2221 gacgcgttca cggccggcaa cctcaagaag gccaagtacg ccgccgaccc gctgcgcgac
     2281 ggctcgctgg agggctacca ggtcttcgaa gtgcccatga ccaccatgac gatgggtgcg
     2341 ctggagtcgg cggagctggg caagaaggac aaggagcgcg cgaagaacat gttcgcgctg
     2401 ggcctggtgt cgtggatgta ctcgcgcccg ctggagtcca ccgagcagtg gctgcggcag
     2461 aagttcgcca agcggccgga gatcgccgag gccaacgtcg ccgtgctcca cgccggctgg
     2521 gcgttcggcg agaccaccga gatcttctcg cacagctacg aggtgcagcc ggcggcgctg
     2581 cccacggggc gctaccgcca gatcaccggc aacaccgcgc tggcctacgg gctcatcgcc
     2641 gcctcggagc gctccggcct gccgatcttc ctgggcagct acccgatcac gccggcgtcc
     2701 gacatcctcc acgagctgtc gcgccacaag gagttcggcg tgcgcaccat gcaggccgag
     2761 gacgagatcg ccggcatcgg cgccgcgctc ggcgcgtcgt tcgcggggat gctcggcgtc
     2821 accacgacgt cggggcccgg cgtggcgctc aagagcgaga ccatcggcct ggccgtcagc
     2881 gtggagctgc cgctggtgat cgtggacatc cagcgcggcg gcccgtccac cggcctgccc
     2941 accaagaccg agcaggccga cctgctgcag gcgatgtacg ggcgcaacgg cgaggcgccg
     3001 gtgcccatcg tcgcgcccgc cacgccgggc gactgcttcg acgccgcgat cgaagcggcg
     3061 cgcatcgcgc tcaagtaccg cacgccggtg ttcctgctgt ccgacggcta cctggccaac
     3121 ggcgccgagc cgtggcgcgt cccggacccg gacgggctgc cggacctgcg cgaggtcgtc
     3181 aacttcgcca ccgtgcccaa ccacgacgac ggcttctggc cgttccagcg cgacccggag
     3241 acgctggcgc ggccgtgggc ggtgcccggc acgcccggcc tggagcatcg catcggcggg
     3301 ctggagaagg ccgacggcag cggcaacatc tcctacgacc cggacaacca ccaccgcatg
     3361 gtggagctgc gcgcggcgaa ggtggcgggc atcgccaagg acatcccgcc gctggaggtg
     3421 gacgaccccg acggccacgc gcgggtgctc gtgatcggct ggggctccac gtacgggccc
     3481 atcaaggcgg cctgccgccg ggcgcgctcg cggggcacgc cggtggcgca ggcgcacctg
     3541 cgccacctca acccgttccc cgcgaacgcc ggcgaggtgc tcgcgcgcta cgaccgggtg
     3601 ctcgtgccgg agatgaacct ggggcagctg cgcatgctgc tgcgcgcgca gttcctggtc
     3661 gacgccgtgg gctacaaccg cgtgcgcggc ctgccgttca cgtcctcgga gctggaggag
     3721 gccatcgtgg ggctggagca ggagggcgcg cgatgaccac ggcgctgccg ctgaccaaga
     3781 aggacttcgt caccgaccag gaagtgcgct ggtgccccgg ctgcggggac tacgcggtgc
     3841 tggccacggt gcagggcgtg ctgccggagc tgggcatcgc ccgggagaac ctggtgttcg
     3901 tgtccgggat cggctgctcc agccgcttcc cgtactacgt cgacacctac ggcttccact
     3961 ccatccacgg ccgcgctccc gccatcgcga cgggggtcgc ggcgtcgcgg ccggacctca
     4021 aggtgttcgt ggtcaccggc gacggcgacg ggctgtccat cggcggcaac cacctgatcc
     4081 acgcgctgcg ccgcaacaac aacctgacca tcatcctgtt caacaaccag atctacggcc
     4141 tcaccaaggg ccagtactcg cccaccagcg agctgggcaa ggtgaccaag tcgacgccgt
     4201 tcgggtcggt ggaccagccg ttcaaccccg tcagcgtcgc gctgggcgcg gaggccacgt
     4261 tcgtcgcgcg ctccatcgac aacgaccggg agcacctggg gcggatggtc aagctcgccg
     4321 cgcagcacaa gggcgcctcg ctgatcgagg tgtaccagaa ctgcaacatc ttcaacgacg
     4381 gcgcgttcga cgccgtgcgc gaccacaagg ccaaccagat ccgcctggag gacggccagc
     4441 cgatccgctt cggccaggac ggcgagatgg gcgtggtcat ggagcacgac ggcacgctgc
     4501 gcatggcgcc ggtggccgag gtgggcgagg agcgcgtgct ggtgcacgac gcgcaccggg
     4561 acgacccggg cctcgcgttc gcgctgtcgc ggctcagctc cgggccgacc ggcccgacgc
     4621 cgatcggggt gttccgccag gtctcccgcc cggtctacgg attggaaatg cagcgccaga
     4681 tcgatgacgc tactgcccgg cgcggccgcg gcgacctcgc ggcgctcctg gccggaaccg
     4741 acacctggga cgtcccgtag cggacgcgac gacgacgagg cacacgatga aggtcctgct
     4801 ggtcaccacc gacgcccgca tcgaggccga cgtgcgcgcg gcgctcatgg gcgacgacgt
     4861 ccacgtcctg caggtcgcca cgccgcagcg cgcgctggcc tacgtgctgg agcgccaggg
     4921 cgagctcgac atcgtggtgg ccgacggcga cacggcgccc accggcggct tcgcgttctc
     4981 ccaccagctc aagcagcgca aggacatggg cgacgacgtg ccgccgatcg tgctgctgat
     5041 cgcgcgcgac gacgatcgct ggatggccaa gtggtcccgt gcggacgcct acatccgcaa
     5101 gcccatcgac ccgttcgacc tggagcaggt ggtgcgcgcg atcgtcgccg gcgaggccat
     5161 ccccgagcgc ccgcgcgtgc acgccgcccc cggcgagttc gaggcgatcc tggccgcgtc
     5221 gaaggtcctg gccggcggcg ccgccgcgag cgggccgatc tcggccgggc cgtgagcggg
     5281 gcgtcgtgac caccgccggg gggacgcagg gggccgcgcc gagctggccc gacctgctgg
     5341 tgcgcatcca gcggcgcgag gacctggacg acgacaccgc ggccgccgcg atgaccgcga
     5401 tcatggaggg ccaggtcacg ccgctgcagg tcgccgcgct gctcatgggc ctgcgcgtca
     5461 agggcgagac cgccgaggag gtggccggct tcgtgcgcac gatgcgccgc
//
LOCUS       DATMKC010000185         4324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_288011, whole genome shotgun sequence.
ACCESSION   DATMKC010000185 DATMKC010000000
VERSION     DATMKC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288011"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1292)
                     /locus_tag="VNU01_05385"
     CDS             complement(135..1292)
                     /locus_tag="VNU01_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWH32086.1"
                     /translation="MVTEAAPATLAEARDAVLGGGALLFAGAGTKLDWLPPARACDTI
                     VRTAGLRRLVAHERDDLTATVEAGMPLAELQRELAGAGQWLAVDPPLAQAGATVGGVF
                     AADDAGPRRLRYGTLRDQVIGCTVVLSDGAVARSGGRVIKNVAGYDLMRPLCGSHGTL
                     GLVTELTVRLHPLPERAVTLRAAAGAHAAAGLALRLLGGVAQPSAVDWTDDTVWVLLE
                     GRGAGLMQRAESVRRAGAALGVHLEELDDAEAEASWAVLRELPAGRPGETVLRAATLP
                     DGFGDLAIVFERAAQRSGLQVALHSHVALGLHTARLSGGTAAAHTGLAGVWREAVAGL
                     GGTPWLRRRVGGVALPASDTPAAAGLMRRLKHALDPDGRCGPGRLDWLEAA"
     gene            complement(1333..2787)
                     /locus_tag="VNU01_05390"
     CDS             complement(1333..2787)
                     /locus_tag="VNU01_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWH32087.1"
                     /translation="MVAAALARRLERLLGPDGVVRDPTALRAYECDGLTSHRQVPDLV
                     ALPRTAQEVAAVVALCAEHGVPFTPRGAGTGLSGGALAAEGGVVVATTRMRDILAVDP
                     ANQRLVAQPGVTNREISAAVARHGLYYAPDPSSQVVCTIGGNVAENSGGAHCLKYGFT
                     THHVLAVELVTPGGEIVTLGSALAESPGYDLRGVVVGSEGTLGIVTAVTVRLLPLPEA
                     VRTLVADFASPAAAGEAVSGMVAAGITPAAVEMLDALAIEACEAATSAGYSLDAGAAL
                     IVELDGPAAEVEAEFAAAERICRDHGAARVRIAGGAQERELIWKGRKAAFAAMGRLAP
                     AYLVQDGVIPRTRLGAVLGRIAELASEAGLRVANVFHAGDGNLHPLVLYDADVAGEAV
                     RAEELSKRIMSLCVDAGGSITGEHGVGVDKACDMPRMFSEEDLAVMARVRGAFDPDGL
                     CNPGKVLPTPRLCGDRPGPYRPHPLEEAGVISQW"
     gene            complement(2787..3596)
                     /locus_tag="VNU01_05395"
     CDS             complement(2787..3596)
                     /locus_tag="VNU01_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWH32088.1"
                     /translation="MAMEAEARTAGLLWSELRSLAHGLAEHEWDKATPCARWTVKDLL
                     AHVTGLQTAMDGTAEQPPAPEGWSPQAEGLSGLDAWTELGVVARRNWSRERLLEELEV
                     AAAGHVARFAAGDPGDDAIGPFGPTTLERLHGFRMFDLWVHLQDFHLALGRPLDLGAQ
                     APGGADACRHVFRGLPALAVKRAGLGDGDRLRVRVEEPAAIDAALVVEGGRAAWDPGA
                     EAADAVEAKPGVLALLLAGRGTPEEWRAEGGLDWRGTLGEAFVQRARVFGA"
     gene            complement(3550..>4324)
                     /locus_tag="VNU01_05400"
     CDS             complement(3550..>4324)
                     /locus_tag="VNU01_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HWH32089.1"
                     /translation="GRGEGAMMAPEPLPTRRERLAVLVEDRLDIPMAILALAWALLVA
                     YELVAPAEQRDELALAGNLVWGVFVVEFAAKLVISGNPGRFLRRRWPSVLFLVLPALR
                     LFRVVRAVRVLRVLPAARVIGSSYRAVGTAQTLLQGRLGFLVTATAVAVFAGGQLLYL
                     LERTANPGIDGLGGALWWAASVAMGAGRPFEPVTLGGRLLALLLSAYSLVVVASVAGV
                     LGAYFVESRAERATLEEAGAEEAGDGDGGGGTDRGPAVE"
BASE COUNT          540 a         1742 c         1580 g          462 t
ORIGIN      
        1 atcctcggcc gcgcctggat ccgtgtcatc caccgctgct ggaccacccg caccccctac
       61 gacgaagccc tccacggcgg cgcccaacgc ctcaacgcgc cgctgatcgc ggcatagggt
      121 tgacatagag ggtgtcatgc ggcctccagc cagtccaggc gtcccgggcc gcagcgcccg
      181 tccgggtcca gcgcgtgctt cagccgccgc atcaggccgg cggccgccgg ggtgtccgac
      241 gccggcagtg ccacgccgcc gacgcgccgg cgcagccacg gcgtgccgcc cagcccggcg
      301 acggcctcgc gccacacccc cgccaggccc gtgtgcgccg ccgcggtgcc gccggacagg
      361 cgggcggtgt gcaaccccag cgcgacgtgg ctgtggaggg cgacctgcag cccggagcgc
      421 tgtgcggccc gctcgaacac gatggccagg tcgccgaagc cgtcgggcag ggtggcggcg
      481 cgcagcacgg tctcgccggg ccgcccggcg ggcagctcgc gcaggaccgc ccaggacgcc
      541 tccgcctcgg cgtcgtccag ctcctccagg tgcacgccca gcgccgcgcc ggcgcgccgc
      601 acggattcgg cgcgctgcat gagccccgcg ccgcggcctt ccagcagcac ccagacggtg
      661 tcgtcggtcc agtccacggc gctgggctgc gcgacgccgc cgagcagccg cagcgccagc
      721 ccggcggcgg cgtgcgcgcc cgccgccgcg cgcagggtga ccgcgcgctc cggcagcggg
      781 tgcagccgca cggtgagctc cgtcaccagg ccgagcgtgc cgtgcgagcc gcacaggggg
      841 cgcatgaggt cgtagccggc gacgttcttg atgacgcgcc cgccggagcg cgcgacggcg
      901 ccgtcggaca gcaccacggt gcagccgatc acctggtcgc gcagggtgcc gtagcgcagc
      961 cgccgcgggc cggcgtcgtc ggccgcgaac acgccgccca cggtggcgcc ggcctgcgcc
     1021 agcggcgggt ccaccgccag ccactgcccg gcgccggcga gctcccgctg gagttcggcc
     1081 agcggcatgc cggcctccac cgtggcggtg aggtcgtcgc gctcgtgcgc gaccagccgg
     1141 cgcagcccgg cggtgcgcac gatcgtgtcg caggcccgcg cgggcggcag ccagtccagc
     1201 ttcgtcccgg cgccggcgaa cagcagcgcg ccgccgccga gcacggcgtc gcgcgcctcg
     1261 gccagggtgg cgggggcggc ctcggtcacc attgccgcgg atccattccg cgcgtgatcg
     1321 gccgcaggtc gatcaccatt ggctgatcac ccccgcctct tccagcgggt gcggccggta
     1381 cgggccgggc cggtcgccgc acagccgcgg ggtcggcagc accttgccgg ggttgcacag
     1441 cccgtccggg tcgaacgcgc cgcgcacgcg cgccatgacc gcgaggtcct cctcgctgaa
     1501 catgcgcggc atgtcgcagg ccttgtccac gccgacgccg tgctcgccgg tgatcgagcc
     1561 gccagcgtcc acgcacaggc tcatgatgcg cttggacagc tcctcggcgc ggaccgcctc
     1621 gcccgccacg tccgcgtcgt acagcaccag cgggtgcagg ttgccgtccc cggcgtggaa
     1681 cacgttggcc acgcgcaggc cggcctcgga ggccagctcg gcgatgcggc ccagcaccgc
     1741 gcccaggcgc gtgcgcggga tcacgccgtc ctgcaccagg tacgcgggcg ccagccggcc
     1801 catcgccgcg aacgccgcct tgcgcccctt ccagatcagc tcgcgctctt gcgccccgcc
     1861 ggcgatgcgc acgcgggcgg cgccgtggtc gcggcagatg cgctcggccg cggcgaactc
     1921 ggcctcgacc tccgccgccg gtccgtccag ctccacgatc agcgccgcgc cggcgtccag
     1981 cgagtagccg gcggaggtgg cggcctcgca ggcttcgatc gccagcgcgt cgagcatctc
     2041 cacggccgcc ggggtgatgc ccgccgcgac catgccgctg accgcctcgc ccgcggcggc
     2101 gggcgaggcg aagtcggcga ccagcgtgcg caccgcctcg ggcagcggca gcagccgcac
     2161 ggtgacggcg gtcacgattc ccagcgtgcc ctccgagccg accacgacgc cgcgcaggtc
     2221 gtagccgggc gactcggcca gcgcgctgcc cagcgtgacg atctccccgc cgggggtcac
     2281 cagctcgacg gccaggacgt ggtgggtggt gaagccgtac ttgaggcagt gcgcgccgcc
     2341 ggagttctcc gcgacgttgc cgccgatggt gcacaccacc tggctggacg ggtccggcgc
     2401 gtagtacagg ccgtggcgcg ccacggcggc ggagatctcg cggttggtga cgccgggctg
     2461 ggcgaccagg cgctggttgg cggggtccac cgccaggatg tcgcgcatgc gggtggtggc
     2521 gacgaccacg ccgccctcgg ccgccagcgc gccgccggac aggccggtgc ccgcgccgcg
     2581 cggcgtgaac ggcaccccgt gctcggcgca cagggcgacc acggcggcga cctcttgcgc
     2641 ggtgcgcgga agggcgacca ggtccggcac ctgccggtgg ctggtcagcc cgtcgcactc
     2701 gtaggcgcgc agcgccgtgg ggtcgcgcac gacgccgtcg gggccgagca ggcgctccag
     2761 gcgccgcgcc agcgcggcgg cgaccatcag gcgccgaaca cgcgcgcccg ctggacgaac
     2821 gcctcgccca gcgtcccgcg ccagtccagg ccgccctcgg cccgccactc ctccggcgtg
     2881 ccgcgcccgg cgagcagcag cgccagcacg cccggcttgg cctccacggc gtccgcggcc
     2941 tcggcgcccg ggtcccatgc cgcgcgcccg ccttcgacca cgagcgccgc gtcgatcgcc
     3001 gccggctcct ccacgcgcac gcgcagccgg tcgccgtcgc ccaaacccgc gcgcttgacg
     3061 gccagcgcgg gcaggccgcg gaacacgtgc cggcaggcgt cggccccgcc gggggcctgg
     3121 gcgcccaggt ccagcgggcg gcccagcgcc aggtggaagt cctgcaggtg cacccacagg
     3181 tcgaacatgc ggaagccgtg caggcgctcc agcgtcgtgg ggccgaacgg gccgatcgcg
     3241 tcgtcgcccg ggtcgcctgc cgcgaagcgg gcgacgtggc cggccgcggc cacctccagc
     3301 tcttccagga gccgctcccg ggaccagttc cgtcgcgcca cgacacccag ctcggtccag
     3361 gcgtccaggc cgctcagccc ctcggcctgc ggcgaccagc cctccggcgc cggcggctgc
     3421 tcggcggtgc cgtccatcgc ggtctgcagg ccggtgacgt gggccagcag gtccttgacc
     3481 gtccagcggg cgcacggcgt ggccttgtcc cactcgtgct cggccagccc gtgggccagt
     3541 gaccggagct cactccacag caggcccgcg gtccgtgcct ccgcctccat cgccatcgcc
     3601 ggcctcctct gcgcccgctt cctccaacgt cgcgcgctcg gcgcgggact cgacgaagta
     3661 cgcgccgagg acccccgcca ccgaggcgac caccacgagg gagtatgcac tcagcagcag
     3721 ggcgaggagg cgcccgccca gcgtgaccgg ctcgaacggc cgccccgcgc ccatggcgac
     3781 gctggccgcc caccacagcg cgccgcccag cccgtcgatc ccggggttcg cggtgcgctc
     3841 cagcaggtac aggagctggc cgccggcgaa caccgcgacg gcggtggccg tgaccaggaa
     3901 gcccaggcgg ccctgcagca gcgtctgcgc ggtgcccacg gcgcggtagg acgagccgat
     3961 cacgcgggcg gccggcagca cgcgcaggac gcgcacggcg cgcacgacgc ggaacagccg
     4021 cagcgccggc agcaccagga acagcacgga cggccagcgc cgccgcagga agcgccccgg
     4081 gttgccggag atcaccagct tggcggcgaa ctccaccacg aacacccccc agacgaggtt
     4141 gcccgccagg gccagctcgt cgcgctgctc cgcgggggcc accagctcgt aggcgaccag
     4201 cagcgcccag gcgagggcga ggatcgccat ggggatgtcg agccggtcct cgacgaggac
     4261 cgccaggcgt tcgcgtcggg tgggcagcgg ctcgggcgcc atcatggcgc cctcacccct
     4321 accc
//
LOCUS       DATMKC010000186         7808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_122210, whole genome shotgun sequence.
ACCESSION   DATMKC010000186 DATMKC010000000
VERSION     DATMKC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122210"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VNU01_05405"
     CDS             complement(<1..217)
                     /locus_tag="VNU01_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32090.1"
                     /translation="MLRMHLLRRLALLAVIAMLATACGDGGEETEPTEDTTETEDGGG
                     ESAEGDGQLKLGYVLPETGPLAFLGPPM"
     gene            333..3014
                     /gene="polA"
                     /locus_tag="VNU01_05410"
     CDS             333..3014
                     /gene="polA"
                     /locus_tag="VNU01_05410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWH32091.1"
                     /translation="MTDSRPVLALLDGHSLAYRAFFALPDDLRTTTGQLTNAVYGFTS
                     MLIKLLAEHRPDGIAVAFDRGRPAERMALLPDYKANRAETPDEFRSQLPLIDEVLHAL
                     AIPQVTREATEADDLIATYATMASREGWDVLIVTGDRDVFQIVDEHVKVLYTRRGISD
                     TVLMDAKAVQDKYDVDPARYPMLAALRGDPSDNIPGVPGVGEKTAAKLLNAFGDLEGI
                     YANIDKVSGKKLPQMLAEHREAVFNGLEVARLYCDLDVPLALDELRMGEVDQDAVRRL
                     FGTLEFRALWTRLSEQVLAVAPEVTEAEGFDADPLRLGAGGVAGWLGDLEPGQPVAVL
                     AFTEGRPPAVRFTAVALAAAGARPATAQLGELAHQDLEALAALLADEARPKYTHNAKR
                     LLHAARERGWDVAGVAFDTELGAYLVRPEQRDYDLESLAQQYLSRRLAAQAGDRGEGQ
                     LAMALEDDWRDRALRAEALLALRDVLAGELDERDQEPLLGRLELPLVPVLADLEHTGI
                     AVDLDVLAEIGDTLADRIAAVREEIFQWAEGEFNCDSPKQLQVVLFERLGLPKTKKIK
                     TGYSTDEQSLAGLIDAHPIVEPLLEYRKLSKLKGTYVDALPPLVNPRTGRIHAEFNQA
                     VAVTGRLSSQHPNMQNMPIRTAEGREIRRAFVPGEGYTSLLVADYSQIELRVMAHLSA
                     DPGLLEAFNSGEDIHTTTAAMVWDLPADAVDGTLRSRIKGMTYGLAYGLSAFGLGQQL
                     RIPPDEARELMDAYFARFPGVRDYLQGVVEQARKDGYTSTLFGRRRYLPDLTSDNRQR
                     RDMAERMALNAPIQGTAADIIKKAMVAVHERMRRESMASQLLLQVHDELVCETAPGEE
                     EALRAVLVEEMSGVADLKVTLDVDTATGPSWAAAEKH"
     gene            3195..4403
                     /locus_tag="VNU01_05415"
     CDS             3195..4403
                     /locus_tag="VNU01_05415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH32092.1"
                     /translation="MSRKKVLVLGANFGGLTAALAVHHELQGDVDVKVVSASDSFLFN
                     PSLIWLPFGKRTARQITFPVAPTFESHGVEFVHAEATRLDLQGHKVETSAGVEDYDYL
                     VIATGYRNDFSAVPGLGPDGNAETITTLADAQKAGAAWRRFLDKPGDVVIGAAQGAGC
                     FGAAYEFLFNMSYQLKRAGLRDRVKLTYVSAEPFLGHFGIGGLPHGESLLGMFMGKER
                     IETVLNVPMDHVDEGRLVLGDGRTVDFSYAMIVPPFEGQQVIRSAPEIADARGYVKVR
                     DTYQAEGFDDVYAVGVAAAVPVPWTTPVPVGIPKTGFPTEQQAHVAARNIAAQIRGEV
                     PSAHKEFGDIPAVCVMDAGNNGVLILADRMLPPRKHGVLIPGPQSHAMKVAFEKYFLW
                     KARHGYVGLP"
     gene            4550..6217
                     /gene="rpsA"
                     /locus_tag="VNU01_05420"
     CDS             4550..6217
                     /gene="rpsA"
                     /locus_tag="VNU01_05420"
                     /inference="COORDINATES: protein motif:HMM:NF005911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWH32093.1"
                     /translation="MAETTTDGTTEAPRQLATAPREERERGEQIVLEEFGSEEEFQKA
                     LDATIKEFDDGSIVEGVVVKVDPEEVLLDIGYKSEGVIPSRELSIKHDVDPFEVVAIG
                     DVIEALVLQKEDKEGRLILSKKRAQYERAWGKIEEIYNNDSTVTGTVIEVVKGGLILD
                     IGLRGFLPASLVEMRRVRDLHPYVGKELECKVIDLDKNRNNVVLSRRKFLEETQSEFR
                     REFLETLQKGEVRKGTVSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPGEIVEVGQE
                     VEVEVLDVDLDRERVSLSLKATQEDPWRQFARQHYVGEFIEGNVTKLVPFGAFVKVEE
                     GIEGLIHISELAEAHVEVPEQVVKVNDPITVKIIDIDDQRRRISLSLKQAVAAGYGEA
                     PEIAAAPAAESFSAETYDAPETGEVTSAMGAALAEAFQQRAGDSGEAAEEAPEAEAAS
                     EAAAEVDEAKAELRQEVGAESEEAVQGEAAEQAPEVETAPEEVAGPAAEAADLTEAGE
                     ADPQPAAEEPKPEAETAADVAEETLEGKPEGETVEGESGEGESGEGE"
     gene            6230..6868
                     /gene="coaE"
                     /locus_tag="VNU01_05425"
     CDS             6230..6868
                     /gene="coaE"
                     /locus_tag="VNU01_05425"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWH32094.1"
                     /translation="MFLVGLTGGIGSGKSTVAARLVEHGAELIDADQIAREVVLPGEP
                     AFERIVAHFGVEILDDDGCIDRPRLGAIVFADPAKRTVLNELTHPAVFERIANELELL
                     QAFDGLVVLDVPLLVEAGAQRGYDAVLVVATKPETQVERLVAHRGMAEDDARARIAAQ
                     APLEDKLKVATHVVWNEGTVDELVAKVDEVAEELCELARQKAAREDASVPND"
     gene            complement(6879..>7808)
                     /locus_tag="VNU01_05430"
     CDS             complement(6879..>7808)
                     /locus_tag="VNU01_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32095.1"
                     /translation="GAVQVLGAELPFDIFEAGGIGWCPQVERFVQIWGAAQFRRDGTW
                     MGGPAPGGMERYAVSRRDGRLVVRESLGVPPRDEPSAYQPPVWRDNECWSRTGHDLDL
                     DQVLLHGEPFADAITVRELRGLRNGSATVFGNAVGRGDGGLELCDELAGTRPPRCADP
                     LVRRGDGGRDLSGYGYEGWFEVRMRHGVPVRVALRAMTRPAGWEAPAPDPRIRVEGPE
                     GRHLTVPAFDFDFADGPDQPLPAGSWSVQLVNHGTQRHGLRIEPGPVLDMRPGRTYIA
                     ITAPFDLPPGDYVAYCPVPGHREAGMEFAFQVR"
BASE COUNT         1198 a         2699 c         2888 g         1023 t
ORIGIN      
        1 tcatgggggg gccgaggaac gccagcgggc cggtctcggg caggacgtag ccgagcttga
       61 gctggccgtc gccttccgcg ctctcgccgc cgccatcctc ggtctcggtg gtgtcttccg
      121 tgggctccgt ctcctcgccg ccgtcaccgc acgccgtagc gagcatggcg atcacggcaa
      181 gcagggcgag ccgtcgcagc aggtgcatcc gcagcattga gcactcctgt cagttcgggg
      241 acctgccaga ggcaggggtc ccgagcctaa cctacggccg ttgtaggagg aagagctttc
      301 ccgtgagggc gccggcgcta gcatcggcgc ccatgacgga ttcccgcccg gtgctcgccc
      361 tgctcgacgg ccacagcctg gcctaccgcg ccttcttcgc gctgccggac gacctgcgca
      421 ccaccaccgg ccagctcacc aacgccgtct acggcttcac cagcatgctg atcaagctgc
      481 tggccgagca ccgtccggac ggcatcgcgg tcgccttcga ccgcggccgc ccggccgagc
      541 gcatggcgct gctgccggac tacaaggcca atcgcgccga gacgcccgac gagttccgct
      601 cgcagttgcc cctgatcgac gaggtgctgc acgcgctggc gatcccgcag gtgacgcgcg
      661 aggcgaccga ggcggacgac ctcatcgcca cgtacgccac gatggcgtcg cgcgagggct
      721 gggacgtgct gatcgtgacc ggcgaccgcg acgtcttcca gatcgtcgac gagcacgtca
      781 aggtgctgta cacgcgccgg ggcatcagcg acacggtgct catggacgcc aaggcggtcc
      841 aggacaagta cgacgtcgac cccgcccgct acccgatgct ggcggcgctg cgcggggatc
      901 ccagcgacaa catccccggc gtgccgggcg tcggggagaa gacggccgcc aagctgctca
      961 acgccttcgg cgacctggag ggcatctacg ccaacatcga caaggtcagc ggcaagaagc
     1021 tgccgcagat gctggcggag caccgcgagg cggtgttcaa cggcctggag gtggcccggc
     1081 tgtactgcga cctcgacgtg ccgctggcgc tggacgagct gcgcatgggc gaggtcgacc
     1141 aggacgcggt ccgccgcctg ttcggcacgc tggagttccg ggcgctgtgg acgcggctgt
     1201 cggagcaggt gctcgcggtg gcgccggagg tcaccgaggc cgaggggttc gacgccgatc
     1261 cgctgcgcct cggcgccggc ggcgtggccg gatggctggg cgacctggag ccgggccagc
     1321 cggttgccgt gctggccttc acggaggggc ggcctccggc cgtgcggttc accgctgtgg
     1381 cgctggcggc ggcgggggcc cggccggcca ccgcccagct cggcgagctg gcccaccagg
     1441 accttgaggc gctggccgcg ctgctggccg acgaggcccg gcccaagtac acgcacaacg
     1501 cgaagcggct cctgcacgcc gcgcgcgagc gcggctggga cgtcgcgggg gtggcgttcg
     1561 acacggagct cggcgcctac ctggtgcgcc ccgagcagcg cgactacgac ctggagtccc
     1621 tggcgcagca gtacctgtcc cggcggctgg cggcgcaggc cggcgatcgc ggcgagggcc
     1681 agctcgccat ggcgctggag gacgactggc gcgaccgggc cctgcgtgcg gaggcgctgc
     1741 tggcgctgcg cgacgtgctg gccggggagc tcgacgagcg ggaccaggag ccgctgcttg
     1801 gccggctgga gctgccgctg gtgccggtgc tggcggacct ggagcacacg ggcatcgccg
     1861 tggacctgga cgtgctggcc gagatcggcg acacgctggc ggaccgcatc gcggcggtgc
     1921 gcgaggagat cttccagtgg gccgagggcg agttcaactg cgactcgccc aagcagctcc
     1981 aggtggtgct gttcgagcgg ctgggcctgc ccaagaccaa gaagatcaag accggctact
     2041 ccaccgacga gcagtcgctg gccgggttga tcgacgcgca ccccatcgtc gagccgctgc
     2101 tggagtaccg gaagctgtcc aagctgaagg gcacctacgt cgacgcgctg ccgccgctgg
     2161 tgaacccgcg caccgggcgc atccacgcgg agttcaacca ggcggtggcg gtgacggggc
     2221 ggctgagctc ccagcacccc aacatgcaga acatgccgat ccgcacggcc gagggccgcg
     2281 agatccggcg cgcgttcgtg cccggcgagg gctacacctc gctgctcgtc gccgactact
     2341 cgcagatcga gttgcgcgtg atggcgcacc tgtccgccga cccgggcctg ctggaggcgt
     2401 tcaactcggg ggaggacatc cacacgacca ccgccgccat ggtgtgggac ctgccggcgg
     2461 acgcggtgga cggcacgctg cgcagccgga tcaaggggat gacgtacggg ctggcctacg
     2521 ggctgagcgc gttcgggctc ggccagcagc tgcgcatccc gccggacgag gcgcgcgagc
     2581 tcatggacgc ctacttcgcg cgcttccccg gcgtgcgcga ctacctgcag ggcgtggtgg
     2641 agcaggcgcg caaggacggc tacacgtcca cgctgttcgg gcgccggcgc tacctgcccg
     2701 acctgacgtc ggacaaccgg cagcgacgcg acatggccga gcgcatggcg ctgaacgcgc
     2761 cgatccaggg caccgccgcc gacatcatca agaaggcgat ggtggcggtg cacgagcgca
     2821 tgcggcggga gagcatggcg tcgcagctgc tgctgcaggt gcacgacgag ctggtgtgcg
     2881 agacggcccc cggcgaggag gaggcgctgc gcgccgtgct ggtggaggag atgtccgggg
     2941 tggcggacct gaaggtcacc ctggacgtcg acacggccac cggcccctcc tgggcggccg
     3001 ccgagaagca ctgaccggct cggcggctcc cgatcccgcc gacggtgtgc gcgtcgcggc
     3061 gcgcgccgcc gccaccgggt cctgaccggc cgaagccggg actgtccgcc cgtccgcggg
     3121 gggacttccg gcacgttcac gccgcggcgg cgcgccgtag ggtcgggaca ggtgctgacg
     3181 ccaaggaggc cgccatgtca cgcaagaagg tcctggtcct gggggcgaac ttcggggggc
     3241 tcacggcggc cctggcggtg caccacgagc tgcaaggcga cgtcgacgtc aaggtcgtgt
     3301 ccgcctcgga cagcttcctg ttcaaccctt cgctgatctg gctgccgttc ggcaagcgca
     3361 cggcgcgcca gatcacgttc cccgtggcgc cgaccttcga gtcgcacggg gtggagttcg
     3421 tgcacgccga ggcgacgcgg ctggatctgc agggccacaa ggtcgagacc tccgcgggcg
     3481 tggaggacta cgactacctg gtgatcgcga ccggctaccg caacgatttc tcggcggtgc
     3541 cgggtctggg accggacggc aacgcggaga ccatcaccac gctcgccgac gcgcagaagg
     3601 ccggtgccgc gtggcggcgc ttcctggaca agcccggtga cgtggtgatc ggcgcggcgc
     3661 agggagcggg ctgcttcggc gcggcctacg agttcctgtt caacatgagc taccagctca
     3721 agcgggcggg cctgcgcgac cgcgtgaagc tcacgtacgt cagcgccgag ccgttcctgg
     3781 gccacttcgg catcggcggc ctgccgcacg gcgagtccct gctgggcatg ttcatgggca
     3841 aggagcgcat cgagacggtg ctgaacgtgc cgatggacca cgtcgacgag ggccgcctgg
     3901 tgctcggcga tggccgcacg gtggacttct cctacgcgat gatcgtgccg cccttcgagg
     3961 gccagcaggt gatccgctcc gcgccggaga tcgccgacgc ccgcgggtac gtcaaggtgc
     4021 gcgacaccta tcaggcggag ggcttcgacg acgtctacgc cgtgggcgtg gcggccgccg
     4081 tgccggtgcc gtggaccacg ccggtgccgg tgggcatccc caagacgggc tttcccaccg
     4141 agcagcaggc gcacgtggcg gcgcgcaaca tcgcggcgca gatccgcggc gaggtgccga
     4201 gcgcgcacaa ggagttcggg gacatcccgg cggtctgtgt catggacgcg ggcaacaacg
     4261 gcgtgctgat cctggccgac cgcatgctgc cgccgcgcaa gcacggtgtg ctgatccccg
     4321 gcccgcagag ccatgcgatg aaggtcgcct tcgagaagta cttcctgtgg aaggccaggc
     4381 acggctacgt cgggctgccg tagcgggcgc accaggggcg gtgtggccgg gctcgcgttg
     4441 acaggtgcac aagcgcacgt gacactcgtg ggaccgcccg accccttcgg gtggttcttc
     4501 gcgctcgccg agcgcgtgtc cggaaaacca aggagaccga gtcatcacca tggctgagac
     4561 caccaccgac ggcaccaccg aagcgccgcg ccagctggcc accgcccctc gcgaagagcg
     4621 cgagcgcggc gagcagatcg tcctcgagga gttcggctcc gaggaggagt tccagaaggc
     4681 cctggacgcg accatcaagg agttcgacga cggttcgatc gtcgagggcg tggtggtcaa
     4741 ggtcgacccc gaggaggtcc tgctcgacat cggctacaag tccgagggcg tcatcccctc
     4801 gcgcgagctg tcgatcaagc acgacgtcga ccccttcgag gtcgtcgcca tcggtgacgt
     4861 catcgaggcc ctcgtcctgc agaaggagga caaggagggc cggctgatcc tgtccaagaa
     4921 gcgcgcccag tacgagcgcg cgtggggcaa gatcgaggag atctacaaca acgactccac
     4981 ggtcaccggc acggtcatcg aggtggtcaa gggcgggctc atcctcgaca tcgggctgcg
     5041 cggcttcctg ccggcctccc tggtcgagat gcgccgcgtg cgggacctgc acccgtacgt
     5101 cggcaaggag ctcgagtgca aggtcatcga tctcgacaag aaccgcaaca acgtggtgct
     5161 gtcccgccgc aagttcctgg aggagacgca gagcgagttc cgccgcgagt tcctcgagac
     5221 cctgcagaag ggcgaggtcc gcaagggcac cgtgtccagc atcgtgaact tcggcgcctt
     5281 cgtggacctg ggcggcgtcg acggcctggt gcacgtcagt gagctgtcct ggaagcacat
     5341 cgaccacccg ggcgagatcg tcgaggtcgg ccaggaggtc gaggtcgagg tgctcgacgt
     5401 ggacctggac cgcgagcgcg tgtcgctgtc gctcaaggcc acgcaggagg acccgtggcg
     5461 ccagttcgcc cgccagcact acgtgggtga gttcatcgag ggcaacgtca ccaagctggt
     5521 gccgttcggc gcgttcgtga aggtggagga gggcatcgag ggcctcatcc acatctccga
     5581 gctcgccgag gcgcacgtcg aggtgcccga gcaggtcgtg aaggtcaacg acccgatcac
     5641 ggtcaagatc atcgacatcg acgaccagcg ccgccgcatc tcgctgtcgc tgaagcaggc
     5701 cgtcgcggcc ggctacggcg aggccccgga gatcgccgcc gcgcccgcgg cggagtcctt
     5761 cagcgccgag acgtacgacg cgccggagac cggcgaggtc acctccgcca tgggcgcggc
     5821 cctggccgag gcgttccagc agcgcgccgg cgactccggc gaggccgcgg aagaggcgcc
     5881 cgaggccgaa gcggcgtccg aggccgctgc cgaggtggac gaggccaagg ccgagctgcg
     5941 ccaggaggtc ggcgccgagt ccgaggaggc cgtgcagggc gaggccgccg agcaggctcc
     6001 cgaggtcgag accgctcccg aggaggtcgc cggaccggcg gccgaggccg ctgacctgac
     6061 cgaggccggc gaggcggacc cgcagcctgc cgccgaggag cccaagcccg aggcggagac
     6121 cgccgcggac gtcgccgagg agaccctcga aggcaagccc gagggcgaga cggtcgaggg
     6181 cgagtcgggc gagggcgagt cgggcgaggg cgagtaggag cctggtgaca tgttcctcgt
     6241 cggcttgacg ggggggatcg gcagcggcaa gtccaccgtc gccgcgcgcc tcgtcgagca
     6301 cggcgcggag ctcatcgacg cggatcagat cgcccgggag gtcgtcctcc cgggcgagcc
     6361 cgccttcgag cgcatcgtcg cgcacttcgg cgtcgagatc ctcgacgacg acggctgcat
     6421 cgaccgcccc aggctggggg cgatcgtctt cgccgacccg gccaagcgca cggtgctgaa
     6481 cgagctgacg catccggcgg tgttcgagcg catcgccaac gagctggagc tgctccaggc
     6541 gttcgacgga ctggtggtcc tcgacgtgcc gctgctggtc gaggccggcg cgcagcgcgg
     6601 ctacgacgcg gtgttggtgg tggcgacgaa gccggagacg caggtggagc ggctcgtcgc
     6661 gcaccgcggc atggcggagg acgacgcgcg ggcgcgcatc gccgcgcagg cgccgctgga
     6721 ggacaagctc aaggtcgcca cccacgtcgt gtggaacgag ggaacggtcg acgagctggt
     6781 cgccaaggtg gacgaggtgg ccgaggagct gtgcgagctg gcgcgccaga aggccgcgcg
     6841 cgaggacgcc agcgtcccca acgactgacc ccgcagagtc agcggacttg gaacgcgaac
     6901 tccatgccgg cctcgcgatg gccgggcacc gggcagtagg cgacgtagtc gcccggcggc
     6961 aggtcgaagg gggccgtgat ggcgatgtag gtcctgcccg ggcgcatgtc cagcacgggc
     7021 cccggttcga tccgcaggcc gtgacgctgc gtcccgtggt tgacgagctg cacggaccag
     7081 ctccccgccg gcagcggctg gtccgggccg tccgcgaagt cgaagtcgaa cgccggcacg
     7141 gtcaggtggc ggccctccgg cccctccacg cggatgcggg ggtccggcgc cggcgcctcc
     7201 cacccggccg gccgggtcat cgcgcgcagc gccacgcgca ccgggacccc gtggcgcatg
     7261 cggacctcga accagccctc gtagccgtac cccgacaggt cgcgcccgcc gtcaccgcgg
     7321 cgcaccagcg ggtcggcgca gcggggcggg cgtgtgcccg ccagctcgtc gcacagctcc
     7381 agcccgccat cgccgcgacc caccgcgttg ccgaagaccg tcgcggagcc gttccggagt
     7441 ccgcgcagct cgcgcacggt gatggcgtcc gcgaacggct ccccgtgcag gagcacctgg
     7501 tcgagatcca ggtcgtgccc ggtccggctc cagcactcat tgtcgcgcca caccggtggc
     7561 tggtaggccg agggctcgtc gcgcggcggc acgcccaggc tctcgcgcac cacgagccgc
     7621 ccgtcgcggc gtgacacggc gtagcgctcc atgccgccgg gcgccggccc gcccatccac
     7681 gtgccgtcgc ggcggaactg cgccgcgccc cagatctgca cgaagcgttc gacctgtggg
     7741 caccagccga tgccgccggc ctcgaagatg tcgaacggca gctccgcgcc gagcacctgg
     7801 accgcgcc
//
LOCUS       DATMKC010000187         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_298046, whole genome shotgun sequence.
ACCESSION   DATMKC010000187 DATMKC010000000
VERSION     DATMKC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298046"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VNU01_05435"
     CDS             complement(<1..579)
                     /locus_tag="VNU01_05435"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWH32096.1"
                     /translation="MADREHKEALYAHVARAGKALAHGKRLELLDLLAQAERSVEALA
                     VATGLSVTSTSAHLQVLRRGGLVQTRRDGNRVHYRLAGDDVARLLVSLRELAADRLAD
                     VERAAREYLGEDDVEPVSREELQRRLRQGDVVVVDVRPAEEYAAGHVRGAVSIPVDEL
                     ERRIGELPPGAEVVAYCRGPLCVYAPQAVGILR"
     gene            671..2023
                     /locus_tag="VNU01_05440"
     CDS             671..2023
                     /locus_tag="VNU01_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWH32097.1"
                     /translation="MRIVPIVDEGLGNSSYVVDLGDGSAVVIDPFRDPAPYLREAERH
                     GLRLRFAAETHLHADFVSGSRELARHGARVVASAAAPVAFPAELLDDGGELDAGGITV
                     RALGTPGHTPEHLSYLLSDGARPLAVFTGGTLIPGGVARPDLLGPEHTESLARAAYHS
                     VRRLLALPDALPVYPTHGPGSFCSAGGGGKRVSTIGEERAANPLLRAATEEEFVRVLL
                     SGLGSYPHYFRELREVNQAGPAVVGDEPPALRALDPAEAERLVRDGAELVDVRDIEAF
                     ARGHVPGSLSIAQRAQFTPWLGWLVRRDRPLVFVGDERTDRRALVWAALSIGYEHLAG
                     ELSGGVDAWAASGRALARLPLVEPEAVDARRGIVDVRQADEWQAGHVDGAEHVELGAL
                     PERAGALPGEPLVVHCAHGERAMTGASVLARAGHRDVVALRGDPRRLAAALGRGVERA
                     "
     gene            2023..>2328
                     /locus_tag="VNU01_05445"
     CDS             2023..>2328
                     /locus_tag="VNU01_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH32098.1"
                     /translation="MGSAAAVLRLGLRHNAGQFFLLVGVNALVGGMVGQERVVLPLLA
                     EREFGVSSFSTLLVFVAAFGLTKAAANLFAGALADRYGRKPVLVAGWLVGALVPPPWR
                     "
BASE COUNT          252 a          859 c          912 g          305 t
ORIGIN      
        1 gcgcaggatg ccgacagcct gcggcgcgta gacgcacagc ggcccccggc agtaggccac
       61 cacctcggcg ccggggggca gctcgccgat gcgccgctcc agctcgtcca ccgggatgga
      121 cacggcgccg cgcacgtggc cggccgcgta ctcctcggcg gggcgcacgt cgaccaccac
      181 cacgtcgccc tggcgcagcc ggcgctgcag ctcctcgcgc gacaccggct cgacgtcgtc
      241 ctcgcccagg tactcgcgcg ccgcgcgctc cacgtcggcc aggcggtcgg cggcgagctc
      301 gcgcagcgac accagcagcc gggcgacgtc gtcgccggcc agccggtagt ggacgcggtt
      361 gccgtcgcgg cgggtctgga ccaggccccc ccggcgcagc acctgcaggt gcgccgacgt
      421 gctggtgacg ctcaagccgg tggcgacggc cagcgcctcg acgctgcgct cggcctgcgc
      481 gagcaggtcc agcagctcca gccgcttgcc atgcgccaat gccttgcccg cgcgcgcgac
      541 gtgcgcgtag agggcttcct tgtgttcgcg atcggccatg cgcacaagtg tagctattcc
      601 aaagaattat ggaatagtgg tcagcgggcg tggcgttgcc caccgcgtga acgagcgagg
      661 agacaccccg atgcgcatcg tgccgatcgt cgacgaggga ctgggcaaca gctcctacgt
      721 ggtcgacctc ggcgacggct cggccgtcgt catcgacccg ttccgcgacc cggcgccgta
      781 cctgcgcgag gcggagcgcc acgggctgcg gctgcgcttc gccgcggaga cccacctgca
      841 cgccgacttc gtctccggca gccgggagct ggcacgccac ggcgcgcgcg tggtcgcctc
      901 ggcggcggcg cccgtcgcgt tcccggccga gctgctcgac gacgggggcg agctggatgc
      961 cggcggcatc accgtgcgcg ccctcggcac gccgggccac acccccgagc acctgagcta
     1021 cctgctcagc gacggcgcgc ggccgctggc cgtgttcacc ggcggcacgc tcatcccggg
     1081 cggggtggcg cgcccggacc tgctgggccc ggagcacacc gagtcgctgg cgcgcgcggc
     1141 ctaccactcc gtgcggcgcc tgctcgccct gccggacgcg ctgcccgtct acccgacgca
     1201 cggcccgggg tcgttctgct cggccggcgg cggcggcaag cgggtcagca cgatcggcga
     1261 ggagcgcgcg gccaatcccc tgctgcgcgc cgcgaccgag gaggagttcg tgcgagtgct
     1321 gctgtcgggc ctgggcagct acccgcacta cttccgcgag ctgcgcgagg tgaaccaggc
     1381 cggtcccgcc gtggtcggcg acgagccgcc cgccctgcgt gccctggacc cggccgaggc
     1441 cgagcgcctg gtgcgcgacg gcgcggagct cgtggacgtg cgggacatcg aggcgttcgc
     1501 gcgcggccac gtgccggggt cgctgtccat cgcccagcgc gcgcagttca cgccgtggct
     1561 gggctggctg gtgcgccgcg accggccgct ggtgttcgtc ggcgacgagc gcaccgaccg
     1621 gcgcgccctg gtctgggcgg cgctgtccat cggctacgag cacctggccg gcgagctgtc
     1681 cggcggcgtg gacgcgtggg ccgcgtccgg ccgcgccctt gcgcgcctgc cgctggtcga
     1741 gccggaggcg gtggacgcgc gacgcggcat cgtggacgtg cgccaggccg acgagtggca
     1801 ggccggccac gtggacggcg ccgagcacgt ggagctgggc gcgctgccgg agcgcgccgg
     1861 ggcgctgccc ggcgagccgc tggtggtgca ctgcgcccac ggcgagcgcg cgatgacggg
     1921 cgcgagcgtg ctcgcgcgcg ccgggcaccg cgacgtcgtg gcgttgcgcg gcgatccgcg
     1981 gcgactcgcg gccgcgctgg gccgcggcgt cgagcgcgcc tgatgggctc tgcggccgcg
     2041 gtgctgcgcc tgggcctgcg gcacaacgcc gggcagttct tcctgctggt cggcgtgaac
     2101 gcgctggtgg gcgggatggt gggccaggag cgcgtggtgc tgccgctgct ggccgagcgg
     2161 gagttcggcg tgtcgtcgtt ctcgacgctg ctggtgttcg tggccgcgtt cggcctgacc
     2221 aaggcggcgg ccaacctgtt cgccggcgcg ctggcggacc ggtacgggcg caagccggtg
     2281 ctggtggcgg ggtggctggt gggcgcgctg gtgccgccgc catggcgc
//
LOCUS       DATMKC010000188         6779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_292211, whole genome shotgun sequence.
ACCESSION   DATMKC010000188 DATMKC010000000
VERSION     DATMKC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292211"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VNU01_05450"
     CDS             <1..577
                     /locus_tag="VNU01_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWH32099.1"
                     /translation="FKMIVGEEKPDSGTFKVGDTVELAYVDQSRDALDPDKTVYTEIT
                     DGQDLLMVGNREMNGRAYVAGFNFKGTDQQKKVGQCSGGERNRIHLAKLLRRGGNLLL
                     LDEPTNDLDVETLRALEEALLEFAGCAVVISHDRWFLDRIATHILAFEGDSQVTWYEG
                     NYADYEEDRRKRLGAEADQPHRIKYKPLVRP"
     gene            complement(574..1662)
                     /locus_tag="VNU01_05455"
     CDS             complement(574..1662)
                     /locus_tag="VNU01_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32100.1"
                     /translation="MTMLVIFGAGASHDVAQMGNIWSTPTSGSLEHWPAASPDEFRPP
                     LTPQLFRGALIDRLLDRYGAASHLIHDARMQVARDAQAFEVEKFLANYAGSSIREDAR
                     AVLATRFLLRDYFWACTQYCLSQLGPGHANNYAGLLGRVNRWCRPRNEPLAVVSFNYD
                     LLAEHAFGNVMNKRLEALPWYVSDSDRLLIKPHGSCNWARALYTGADEVRARYIDHVT
                     AAQAALREWEWTERHSDEIELVPRLGSGALPTVPALALPIEGKPGPVCPQTHLDALAR
                     TLDGPLSVITVGWRGADDWFLDWLGEHTARLRARALICMAGSRAKDDGHQLAAKLQGK
                     LTDCEAALHLEGFSKLQTSTELAAFLED"
     gene            complement(1872..2285)
                     /locus_tag="VNU01_05460"
     CDS             complement(1872..2285)
                     /locus_tag="VNU01_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWH32101.1"
                     /translation="MQSMLVDGWSTLGRTLLVTVLGYTALLVAVRLGGKRTLAKLNAF
                     DWIVTVAFGSALASVATSQDVSVAEGAVVFGRLLEEPMRRMRVTEVEIHQAVREAGLA
                     SPTQAATVVLETNGGLSVITETSPDSAAMGNVEGW"
     gene            complement(2320..2748)
                     /locus_tag="VNU01_05465"
     CDS             complement(2320..2748)
                     /locus_tag="VNU01_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWH32102.1"
                     /translation="MNLGYMLAAVAGLLIAAQVTIMGRNAPRVGPFTVALLVHVGGLV
                     IALVVMFLRRGWGEALESAAGGWWVIPGVAGFFILSCLSLASARVGAAVALALSVATQ
                     LGAGLVLDSRLGIAPINLKATAGIALIVAGTYLVAARAPA"
     gene            complement(3027..4172)
                     /gene="cysS"
                     /locus_tag="VNU01_05470"
     CDS             complement(3027..4172)
                     /gene="cysS"
                     /locus_tag="VNU01_05470"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWH32103.1"
                     /translation="MDDALRLRDTRTGELRPLDAGPVVRLYVCGITPYDATHLGHAFT
                     YVAFDALIRYLEHRGHDVRYVRNVTDVDDDIFRVSRERQEPAEAIAARELDRFTADLK
                     AMQCRPADVEPYASAHVPSMVASVEALCANGSAYALPDGRVYFDTAASDRYGRFAKLD
                     RDEMLRQFAEKGGDPDAPGKRDPLDFLLWQPSAEGEPSWDSPWGAGRPGWHIECSVMA
                     IDELGPTIDIHGGGSDLVYPHHEAEICQAEHVTGQEFSRFWLHTGMVALDGVKMSKSL
                     GNLVFVSELRERFDPLAVRRYLLEHHYRQDWGYDEAELVAAAAGVRRWADVADQGGAL
                     PEAAAAFHEAMRSDLDTPGAVRAVDEAAAAGAGATVRELAGILGFPL"
     gene            complement(4478..5200)
                     /locus_tag="VNU01_05475"
     CDS             complement(4478..5200)
                     /locus_tag="VNU01_05475"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWH32104.1"
                     /translation="MALPASRRRPVSKSGRALPRVPGVRHEQQWWAEGAVVAGVDEVG
                     RGAWAGPVTYAAVVLPADRRMYKLRDSKTLDAPRREELAARITDFAVAVGLGHVTNDE
                     IDRIGMSQACRLAARRAVDALPVRPEVCLIDGNWDFLSGYGTRNVRIVHGDAHSASIA
                     AASIVAKVSRDGLLTEACGQFPPYGFSRNKGYPSPEHRAALLKHGPCVLHRHSWAPIA
                     ALAQGRLPFDDDWDAASEDQGA"
     gene            complement(5210..5560)
                     /locus_tag="VNU01_05480"
     CDS             complement(5210..5560)
                     /locus_tag="VNU01_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32105.1"
                     /translation="MSQPQSDADAEEAKLAVRVTADRILCGTQKDTDRRTKAGILIPA
                     TAKSADRRGVWADVTEIGPLVRTIEPGDRVLFLPEAAIEVDVRGEVFLIIRERDVHAV
                     ASQEREAGSTGLYL"
     gene            5622..6260
                     /locus_tag="VNU01_05485"
     CDS             5622..6260
                     /locus_tag="VNU01_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32106.1"
                     /translation="MRRFAVVLAVLAATACQPVDDVPRDPRGTVERVTETPLDAPVTG
                     MPSSLEDLRARAWPEALAWQDEPVLAEILVDVEASGGWRRAQLTYLAADADRLLTVTV
                     TADGMADELTTLAALQLHPVSRRGMAEVPELPEGVLQPPELVRMGRAELRECGIRDTP
                     TGVLYASGAPAAWNGRRWTAPPVWTATVTSDGGAVQLDPVSGEVLGCVEAGA"
     gene            6268..>6779
                     /locus_tag="VNU01_05490"
     CDS             6268..>6779
                     /locus_tag="VNU01_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH32107.1"
                     /translation="MVVEPAGPGRWADVERLFGTNGACGGCWCMYPRLKASEFDERKG
                     EGNRAALRELVEAGTEPGLLASRDGEPAGWVAIGPREQYGRLSRSPVCKPVDDLAGVW
                     SVVCFFIPRANRRQGLSKVLLDAAVSHAFARGAAAVEGYPVEPQKPDSPDVFAWWGTA
                     QVFRAAGFTEL"
BASE COUNT         1006 a         2300 c         2505 g          968 t
ORIGIN      
        1 gttcaagatg atcgtgggcg aggagaagcc cgactccggc acgttcaagg tcggcgacac
       61 ggtggagctg gcgtacgtgg accagagccg cgacgcgctg gacccggaca agaccgtcta
      121 caccgagatc accgacggcc aggacctgct catggtcggc aaccgcgaga tgaacgggcg
      181 cgcctacgtc gccggcttca acttcaaggg aaccgaccag cagaagaagg tgggacagtg
      241 ctccggcggc gagcgcaacc gcatccacct ggccaagctg ctgcgacggg gcggcaacct
      301 gctgctgctg gacgagccga ctaacgacct ggacgtggag acgctgcgcg cgctggagga
      361 ggcgctgctg gagttcgcgg gctgcgcggt ggtgatctcc cacgaccgct ggttcctgga
      421 ccgcatcgcc acgcacatcc tggcgttcga gggcgacagc caggtcacct ggtacgaggg
      481 caactacgcc gactacgagg aggaccgccg caagcgcctc ggcgccgagg ccgaccagcc
      541 ccaccgcatc aagtacaagc ccctcgtccg gccctagtcc tcgaggaacg ctgcgagttc
      601 ggttgatgtc tgtagcttgc tgaatccttc aagatggagc gccgcttcgc agtcggtcag
      661 tttgccttgc agtttcgcag caagctgatg tccatcgtcc ttggctcggc tccccgccat
      721 gcagatcaag gctcgcgcgc gcaggcgcgc cgtgtgctct cccagccagt cgaggaacca
      781 atcgtcggcg ccgcgccaac ccacggtgat caccgacaag ggcccgtcca acgtgcgggc
      841 aagcgcatcc aagtgcgttt gggggcatac gggaccgggc ttcccctcta tcggcagggc
      901 gagggcgggt accgtcggca atgcaccaga gcctagtcgt ggcacaagct cgatttcatc
      961 actgtgccgc tctgtccact cccattcgcg cagagcggct tgcgcggcgg tcacgtgatc
     1021 gatgtatcga gctcttacct cgtccgctcc ggtgtagaga gcgcgcgccc agttgcagct
     1081 accgtgcggc ttgatgagta ggcggtctga gtcgctgaca taccagggca acgcctccag
     1141 gcgcttgttc atcacgttcc caaaggcgtg ctcagccaag aggtcatagt tgaaagagac
     1201 aacggcgagg ggttcgttgc gagggcggca ccacctgttg acgcggccga gcaggccggc
     1261 gtagttgttg gcatggcccg gcccgagctg agaaaggcag tactgcgtgc aggcccaaaa
     1321 gtagtcacgt aggagaaagc gtgtagccag tacggcccgg gcatcctcgc ggatagagga
     1381 ccccgcgtag ttcgcgagaa acttctccac ttcaaaggct tgcgcgtccc ttgccacttg
     1441 catgcgtgcg tcgtggatca agtgagaggc cgcgccgtat cggtcgagga gccgatcgat
     1501 aagagcaccg cggaagagct ggggtgtcag cggaggtcgg aactcgtccg ggctggctgc
     1561 tggccagtgt tccaacgaac cgctggtggg ggtactccaa atgttgccca tttgggccac
     1621 gtcgtgtgaa gcacccgccc cgaagatcac cagcatcgtc acggctgtct cctcccgtcg
     1681 gtccgtggcg gagtgtgcca gccgagtgag acatgggctt gtggacgagg cggggttggg
     1741 gacgctcggc cgcaagattg cggctcaggg tccccaaacc ggcgctgtgc acaagggcgt
     1801 attgggtcgg atcgggtgcg agggtgcacc ggatccgacc caataacgcg atgtgcacag
     1861 ggtgggcggc gctaccagcc ttcgacgttg cccatcgcgg cgctgtccgg cgacgtctcg
     1921 gtgatcacgg agaggccgcc gttggtctcc agcaccacgg tcgccgcctg ggtcggtgac
     1981 gcgaggccgg cctcgcgcac cgcctggtgg atctccacct cggtcacgcg catccgccgc
     2041 atgggctctt cgagcagccg gccgaagaca acggcgccct cggccaccga tacgtcctgc
     2101 gacgtggcga cgctggccag cgccgacccg aacgccaccg tgacgatcca gtcgaaggcg
     2161 ttgagcttcg ccagcgtgcg cttgccgccg aggcgcaccg ctaccagcag cgccgtgtag
     2221 cccagcaccg tgacgagcag cgtgcggccc agcgtggacc agccgtcgac cagcatgctc
     2281 tgcatgtcca cgggctaccc acccgggggc gggcggacgc taggccgggg cgcgggcggc
     2341 gaccaggtag gtgcccgcca cgatcagcgc gatccctgcg gtggccttga ggttgatcgg
     2401 cgcgatgccc aggcgcgagt ccagcaccag gccggccccg agctgcgtcg ccaccgacag
     2461 ggccagcgcc acggcggcgc ccacgcgcgc cgacgccagc gacaggcagc tcaggatgaa
     2521 gaagccggcc acgcccggga tcacccacca gccgccggcc gccgactcca gggcctcgcc
     2581 ccagccgcgc cgcaggaaca tcaccacgag ggcgatgacg aggccgccga cgtgcaccag
     2641 cagggccacg gtgaacgggc cgacccgcgg ggcgttgcgc cccatgatgg tgacctgcgc
     2701 cgcgatgaga aggccggcca cggccgccag catgtacccg aggttcaccg ttgctccgcg
     2761 tgtcgaaggg gacaaagaca cgccaggatg ccacgccggg gagttcacgc caccgacgcc
     2821 ggtccccacg gaggtcccgg aaaagatcga ggcccccgtg gtcgggcggg ggcctcgaag
     2881 tgcgcatcga cgggttgcgg gcgggcccgt ctggcacatt cgcttcgccg gtgtgtatgc
     2941 ccccgcccgg gacgcccaaa ccgggattcc tggaaaagtt tcgtaaaggt cggtcatcga
     3001 cccggacggg ggtgcgggtg ttccccctac agggggaatc cgaggatgcc ggcgagctcg
     3061 cgcaccgtcg cgccggcccc ggcggcggct gcctcgtcca cggcccggac cgcgccgggc
     3121 gtgtcgagat cgctgcgcat cgcctcgtgg aacgccgccg cggcctccgg aagcgcgccg
     3181 ccctggtccg cgacgtcggc ccagcgccgt acgcccgcgg ccgccgcgac gagctcggcc
     3241 tcgtcgtagc cccagtcctg gcggtagtgg tgctccagca ggtagcgccg cacggccagc
     3301 gggtcgaagc gctcgcgcag ctcgctgacg aagaccaggt tgcccagcga cttggacatc
     3361 ttgaccccgt ccagcgccac catcccggtg tgcagccaga agcggctgaa ctcctggccc
     3421 gtcacgtgct cggcctggca gatctcggcc tcgtggtgcg ggtacaccag gtcgctgccg
     3481 ccgccgtgga tgtcgatggt cgggcccagc tcgtcgatcg ccatgacgga gcactcgatg
     3541 tgccagccgg gacgccccgc gccccatggc gagtcccagg acggctcgcc ctcggcgctg
     3601 ggctgccaca gcaggaagtc cagcgggtcc cgcttgcccg gggcgtccgg gtcgccgccc
     3661 ttctcggcga actggcggag catctcgtcg cgatccagct tcgcgaagcg cccgtagcgg
     3721 tcggaggcgg cggtgtcgaa gtacacgcgc ccgtcgggca gcgcgtaggc gctgccgttg
     3781 gcgcacagcg cctcgaccga cgccaccatc gacggcacgt gcgccgaggc gtagggctcg
     3841 acgtcggcgg ggcggcactg catggccttg aggtccgccg tgaagcggtc cagctcgcgt
     3901 gccgcgatgg cctccgccgg ctcctggcgc tcacgcgaga cccggaagat gtcgtcgtcc
     3961 acgtcggtga cgttgcggac gtagcgcaca tcgtggccgc ggtgctccag gtagcggatg
     4021 agggcgtcga acgcgacgta ggtgaaagcg tggcccaggt gcgtggcgtc gtacggcgtg
     4081 atcccgcaga catacaggcg caccacaggt cccgcgtcga gcgggcgcaa ctcgccggtg
     4141 cgggtgtccc gcagtcgcag ggcgtcgtcc atggccgccc agcgtaacgc cgccgcgtgc
     4201 gacgccattc cagcgccgcg gcgcccggag cgcacggcga ggcgcccgaa cggccgggaa
     4261 aaggggccgg ccgcctgagc agtcatcagg cggccggcgc agaggtgctt ggggggtgcc
     4321 ctctgtatgt ggacggatcc cgaggtgggg tcggggtttc cgttcgtgtc gggagcttcc
     4381 gaggtgggtg tcagtcgctc cgacaggcac aagaatgcgc ccgacggagg cgcggttcca
     4441 tggcgaacta ggacggtttc cgcctggtca caaatgacta ggcgccctgg tcctcgctcg
     4501 cggcgtccca gtcgtcgtcg aacggcagcc gaccctgcgc cagcgcggcg atcggcgccc
     4561 agctgtggcg gtgcagcacg caggggccgt gtttcagcag cgcggcgcgg tgctccggcg
     4621 acgggtagcc cttgttgcgc gagaagccgt agggcgggaa ctgcccgcac gcctcggtca
     4681 gcagcccgtc gcgcgagacc ttggccacga tggacgcggc cgcgatggag gcggagtgcg
     4741 cgtcgccgtg gacgatgcgc acgttgcggg tgccgtagcc ggacaggaag tcccagttgc
     4801 cgtcgatgag gcagacctcc ggccgcacgg gcagcgcgtc gacggcgcgg cgcgcggcca
     4861 ggcggcaagc ctggctcatg ccgatgcggt cgatctcgtc gttggtgacg tggcccaggc
     4921 cgacggccac ggcgaagtcg gtgatgcgcg cggcgagctc ttcgcgccgc ggcgcgtcca
     4981 gcgtcttgga gtcgcgcagc ttgtacatgc gccggtcggc cggcagcacc acggcggcgt
     5041 aggtgacggg gcccgcccac gcgccccggc cgacctcgtc gacgccggcc accaccgcgc
     5101 cttcggccca ccactgctgc tcgtggcgca cgccgggcac gcgcggcagg gcgcggccgc
     5161 tcttgctgac cgggcggcgg cggctcgcgg gcagggccat gggcgcggcc tacaggtaca
     5221 ggccggtgga gccggcttcg cgttcctggc tggcgaccgc gtggacgtcg cgctcgcgga
     5281 tgatgaggaa cacctcaccg cgcacgtcca cttcgatcgc cgcctccggc aggaacagca
     5341 cgcggtcgcc cggctcgatc gtgcgcacca gcgggccgat ctccgtgacg tccgcccaca
     5401 ccccgcggcg gtcggccgac ttggccgtgg ccgggatcag gatgccggcc ttggtgcgcc
     5461 ggtcggtgtc cttctgggtg ccgcacagga tgcggtcggc ggtgacgcgg acggcgagct
     5521 tggcttcctc ggcgtcggcg tcggactgcg gctgggacat gcagcgagca taaccggcgt
     5581 ccgccgcggg gctgtcgccg ctggcgcata gactcgcggc catgcgccga ttcgccgtcg
     5641 tcttggccgt gctggcggcc accgcgtgcc agccggtcga tgacgtgccg cgggatcccc
     5701 gcggcacggt ggagcgcgtc accgagacgc cgctggacgc gccggtcacc ggcatgccgt
     5761 cgtcactgga ggacctgcgg gcgcgcgcgt ggccggaggc gctggcgtgg caggacgagc
     5821 cggtgctggc ggagatcctg gtcgacgtcg aggcttccgg cggctggcgg cgggcgcagc
     5881 tgacgtacct ggccgcggac gccgaccggc tcctgacggt gaccgtcacg gcggacggca
     5941 tggcggacga gctcaccacg ctggcggcac tgcaactgca cccggtgagc cggcgcggca
     6001 tggccgaggt cccggagttg ccggaaggcg tgttgcagcc gcccgagctg gtgcggatgg
     6061 gccgggcgga gctgcgcgag tgcgggatcc gcgacacgcc gacgggcgtg ctgtacgcca
     6121 gtggcgcgcc ggcggcctgg aacggccggc gctggacggc gccgccggtg tggacggcga
     6181 cggtgaccag cgacgggggc gccgtgcaac tcgacccggt ctccggggag gtgctgggct
     6241 gcgtcgaagc gggggcctga gatgggaatg gtggtggaac cggccgggcc tggacggtgg
     6301 gccgacgtcg agcggctgtt cgggacgaat ggcgcctgcg gcggctgctg gtgcatgtac
     6361 ccgaggctga aggccagcga gttcgacgag cgcaagggcg agggcaaccg cgcggcgctg
     6421 cgcgagctgg tggaggcggg cacggagccc ggcctgctcg cctctcgcga cggcgagccg
     6481 gccggctggg tggcgatcgg gccgcgcgag cagtacgggc gcctgtcgcg ctcgccggtg
     6541 tgcaagcccg tcgacgacct ggcgggcgtg tggtccgtgg tgtgcttctt catcccgcgg
     6601 gccaatcgcc gccagggact gagcaaggtg ctgctggacg cggcggtgag ccacgcgttc
     6661 gcgcgtggtg ccgccgccgt ggagggctac ccggtggagc cgcagaagcc ggacagcccc
     6721 gacgtcttcg cctggtgggg cacggcgcag gtcttccgtg ccgccggctt cacggagct
//
LOCUS       DATMKC010000189         3322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_128426, whole genome shotgun sequence.
ACCESSION   DATMKC010000189 DATMKC010000000
VERSION     DATMKC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128426"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..495)
                     /locus_tag="VNU01_05495"
     CDS             complement(139..495)
                     /locus_tag="VNU01_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32108.1"
                     /translation="MRLLGPVRLFRDGHLTPVPAGRCQKLLALLVLTGGSAHRDALCA
                     ALWPDARVRAGHRSLRTLLTRLRRATGLRIEREGDLVVVQDALWCDVEEFIRTASAAA
                     ATDQDRVLGQGADTAS"
     gene            complement(841..1167)
                     /locus_tag="VNU01_05500"
     CDS             complement(841..1167)
                     /locus_tag="VNU01_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32109.1"
                     /translation="MMGTITERQRQRLRQWADRHMDQETADIFMESTPPTGWGDVATK
                     QDLDVLEMRLSAKIDREIGEVRAAQTTHLITIVVAILVAMMGNTVLPHYFPPPSPQIV
                     VQVPSQ"
     gene            complement(1524..2033)
                     /locus_tag="VNU01_05505"
     CDS             complement(1524..2033)
                     /locus_tag="VNU01_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32110.1"
                     /translation="MDRACFELRLFGPVRLSRDGEHTPVPAGRCQEVLALLVLNGGSA
                     HRGALCAALWPDARVKASQNSLRSLLTRLRRATGLRIEREGDLVVVRDAVWCDVEEFI
                     RTATAAVATVDDVATRNRLAVRAQELWRGAPLDSWRYEPWAAEVRVKAFRLQEALWAM
                     LGQDAGTAS"
     gene            2341..2583
                     /locus_tag="VNU01_05510"
     CDS             2341..2583
                     /locus_tag="VNU01_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWH32111.1"
                     /translation="MEDITVHELRERADELLDRVAAGETITITRDGTPVAELRPLAAT
                     KPTLTMDEILERFRQLEPDDPQEFRRELDAILDPYA"
     gene            2604..3002
                     /locus_tag="VNU01_05515"
     CDS             2604..3002
                     /locus_tag="VNU01_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWH32112.1"
                     /translation="MLDSNTIILLSRLGPGTPMPRQALASTITFAELCVGPVVATDPA
                     VVAIRQSEVNLAEKSFELLPFDVASARTYAGVVASLRAAGRKGASRTTDAMIAAVAIA
                     NGLPLYTCNPADFAGIDGLEVVPIPHPNTP"
     gene            complement(3018..>3322)
                     /locus_tag="VNU01_05520"
     CDS             complement(3018..>3322)
                     /locus_tag="VNU01_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32113.1"
                     /translation="WADEAMGRERADTLMKGIPPAGWGDVATKQDLEVLESRFSAKLA
                     DEIGGVHKEIGKVREAQTTHLITIVVAILVAMMGNTILPHYFPPPSPQIVVQVPSQ"
BASE COUNT          539 a         1159 c         1141 g          483 t
ORIGIN      
        1 gccttggacg cgcggagcgg cgtgcccgtt gtgtccaacg gccggcgcga ggagcgcttg
       61 cacgtcagcc gcgtaggcta acaccggcaa gcgcgacggc cagccgatcc cgcaagatcc
      121 aaacgacccg gttccgcgtc aagaagcggt gtcggcgccc tgcccgagca cgcggtcctg
      181 gtcggtggcc gccgcggcgc tggcggtgcg gatgaactcc tcgacgtcgc accacagcgc
      241 gtcctggacg acgaccaggt cgccctcacg ctcgatgcgc aggccggtgg cgcggcgcag
      301 ccgggtgagc agcgtgcgca gcgagcggtg gcccgcccgg acgcgggcgt cgggccacag
      361 cgcggcgcac agggcgtcgc ggtgcgccga gcccccggtg aggaccagca gggccagcag
      421 cttctggcat cggccggccg gcaccggcgt gagatgaccg tcccggaaca ggcggaccgg
      481 tccgagcagc cgcacttcta gagacgctcg ctccacgtcg ctccccctgc agcgccgtga
      541 gcccgcgagt gtgaacgaag cggtgtgtgg gctcagccga acagtgacgc cgtcgagtat
      601 ggcagaggca tcgcccgtcg gcaggacccc atgtggatga ccgacggcag gtgtcgcacc
      661 ccgccaactg cgggaccagt tgtgtccggt agccgactca actcgtcccg gagatcgagc
      721 cgaccggcct cggctactgc gagggaacct gaccgcagaa ccccgcacgt cggtgctgtg
      781 gatacgcggc atcctgctga gagaggacac gaatcgtccc ggagatcggg caggtcttgg
      841 ctactgcgag gggacctgga cgacgatttg cggggacggc ggcgggaagt agtgcggcag
      901 gaccgtgttc cccatcatcg cgacaaggat cgcgacgacg atcgtgatca gatgcgtggt
      961 ctgggccgcg cgcacctcgc cgatctccct gtcgatcttg gccgacaagc gcatttccag
     1021 aacgtcgagg tcctgcttgg tcgcgacatc gccccagccg gtcggcgggg tgctctccat
     1081 gaagatgtcg gcggtctcct ggtccatgtg ccgatccgcc cactggcgca accgctggcg
     1141 ttgacgctcg gtgatcgtgc ccatcatcga ctccccgcag acggcgaaca agggaccgcc
     1201 gcgaacatac gaagcagcgg cgaaacacgg catccgtcat ccacaggacg ccgccgagct
     1261 ccgcccacgg acctccaggc gtccggcggg acctggcgag caaccatgcc gtctctggtg
     1321 tgacgccccg cttgccccgc accagctcgt tgacccgctg cacagggatc cccaggtggc
     1381 gggcgagcgc gacctgggtc agcccgagcg gcgcgaggaa ctcctcgctg agggcctcgc
     1441 ccggatgagt cgggatgcgc tttctcggca gcatgacgag ctccctagtg gtagtcgatg
     1501 atctcgacga ctgacgaacc gagtcaggaa gcggtgccgg cgtcctgccc gagcatggcc
     1561 cacagtgcct cttgaagccg aaacgccttc acgcggacct cggcggccca gggctcgtag
     1621 cgccaggagt ccagcggcgc gccgcgccac agctcctgtg cccgcacggc cagccggttc
     1681 cgcgtcgcga cgtcgtcgac ggtggccacc gcggccgtgg cggtgcggat gaactcctcg
     1741 acgtcgcacc acaccgcgtc ccgaacgacc accaggtcgc cctcgcgctc gatgcgcagc
     1801 ccggtggcgc gccgcagccg cgtgagcagg ctgcgcaggg agttctggct ggccttgaca
     1861 cgggcgtcgg gccacagcgc ggcgcacagc gcgccgcggt gcgccgaacc cccgttgagg
     1921 accagcaggg ccagcacctc ttggcaccgg ccagccggca ccggcgtgtg ctcaccgtcc
     1981 cgggacaggc gcaccggccc gaacagccgc aactcgaaac acgctcgatc cacgtcgctc
     2041 ccctgccgcg ccgtgagccc gagagtgaaa gagcggtgtg tggactcagc cgatcagtga
     2101 cgccgtcgag tatggcagag gcgtcacccg ccggcaggac cgctgtggtt gaccgacggc
     2161 aggcgccgca ccccgccaac tgcgggaccg gttgtgtccg atagcggaca caagtcgtcc
     2221 cggagatcga gccccgccgt cggggctgtg gatacgcggg tgggcgccgg gcgcggtcgg
     2281 catatcgtgc ggacagccac accattgacg tggtcgaagc gtccgttcac gctcaagccg
     2341 atggaggaca tcaccgtgca cgaactgcgc gagcgcgccg acgagctgct cgaccgcgtc
     2401 gccgcgggcg agaccatcac catcacccgc gacggcaccc cggtcgccga actgcggccc
     2461 ttggcggcta ccaagccgac gctcacgatg gacgagatcc tggagcggtt ccggcaactg
     2521 gagccggacg atccccagga gttccgccgt gagctcgacg cgatcctcga cccctacgca
     2581 tgacttggcg ctcaccgcag gggttgctcg acagcaacac gatcattttg ctgtcgcggc
     2641 tcggtcccgg cacacccatg ccgcgccagg cgctggcgtc cacgatcaca ttcgccgagt
     2701 tgtgcgtcgg cccggtcgtt gcgacggacc cggccgtggt ggccatacgg cagtccgaag
     2761 tgaatttggc cgagaagtcg tttgagctgt tgccattcga cgttgcgtca gccaggacat
     2821 atgcgggtgt ggtcgcgtcg ttgcgcgccg cggggaggaa aggcgcgtcg cggacgaccg
     2881 atgcgatgat cgcggcggtg gcgatcgcca acggtctgcc gctctacacg tgcaacccgg
     2941 cggacttcgc cggcatcgac ggcctggaag tcgtgccgat cccccacccc aacacgccgt
     3001 aaccgggccg gtcttggcta ctgggagggg acctggacga cgatctgcgg ggacggcggc
     3061 gggaagtagt gcggcaggat cgtgttgccc atcatcgcga cgaggatcgc gaccacgatc
     3121 gtgatgagat gcgttgtctg tgcctcgcgc accttgccga tctccttgtg gacgccgccg
     3181 atctcgtcgg cgagtttggc cgagaagcgc gactccagaa cctcgaggtc ctgcttcgtc
     3241 gcgacatcgc cccagccggc cgggggaatg cccttcatca gcgtgtcggc tcgctcacga
     3301 cccatggcct cgtccgccca ct
//
LOCUS       DATMKC010000190         3365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_240904, whole genome shotgun sequence.
ACCESSION   DATMKC010000190 DATMKC010000000
VERSION     DATMKC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240904"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1079)
                     /locus_tag="VNU01_05525"
     CDS             complement(72..1079)
                     /locus_tag="VNU01_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWH32114.1"
                     /translation="MSALFAATQSTDVQQFASFEVPLWVWGALTAAIAIMLLVDLLLV
                     HRKPHVISFREAAIESAVWITLGLAFVGVMAWWQGGQAAGEYLAGYLIEKSLSIDNVF
                     VWAVLFGYFAVPAAYQFRVLFWGIFGALVMRAMFIFAGVALIDRFDWVLYVFGAFLLF
                     TAVKIARHSGAEVHPEQNPVLKLVRRVVPTTSHYDGQKLFTKENGKRLATPLFTVLVL
                     VEATDVIFAVDSVPAILAVSREPFIVFASNAFAILGLRSLYFLLAGMAGKFRYLHVGL
                     GVILAFVGVKMLLANVYHMPTTISLGVIAAVLAVTIWASLRADKRDGAAPPPPPGIEQ
                     H"
     gene            complement(1257..2144)
                     /gene="sigJ"
                     /locus_tag="VNU01_05530"
     CDS             complement(1257..2144)
                     /gene="sigJ"
                     /locus_tag="VNU01_05530"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HWH32115.1"
                     /translation="MRDQAAAFEPHRAHLFGVAYRLLGEVGSAEDAVQEAFLRFRRAP
                     EDLAEPRAWLTTVVTRLCLDELRSARARREVYVGPWLPEPLVSPAADAGTDPAQRAED
                     VESLSMALMVVLESLSPLERAVFVLREVFGYDYAELAAMLERSEAAVRQLAHRAREHV
                     QARRPRFDRDDERRRTAVEQFLMATLTGDLTGLMATLTEDALLLSDGGGVVHAARKPI
                     RGPERIARFFLGLASKYMTPAHRAEIATVNGSVGLLLWDGDHIDNVIGFDVAGGKVAR
                     IHVVRNPAKLGRVQAALAD"
     gene            complement(2141..>3365)
                     /locus_tag="VNU01_05535"
     CDS             complement(2141..>3365)
                     /locus_tag="VNU01_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH32116.1"
                     /translation="FEGRALGGMAASEDHGGYLWNWGGHALYRKGPAVPVLRELGVRP
                     RGAVIRGAAFMEALHGGAIVPAPFSARTALSPLLDGADRVDMARAMAAVPRDARGARL
                     RALDHVTLSAWLDQHVTRERARELILALVRLSSFTHDPHRQAASAAVRQLQLALHGVW
                     YLHGGWQQLVDGLRQAAQAAGVDVREHCRVDAVEHDERVRAVRLADGARVAVDGAVVA
                     AGGPRAAARLLDGPGAGVVGGWAGRAVPVRAATYELNLARVPSSPHVWVLGVDQPVYV
                     NVHSHFAAGVAPQGRTLVQVLRYLGPREPSPAYRAEIDAALDLALPGWRDDVAGDRYL
                     PGLTVMHDTPAAAAGGLAGRPGPLVPGVEGLAVVGDWCGRRGILADAALASAKLAATA
                     LSAGAARGAGMEAVA"
BASE COUNT          488 a         1287 c         1201 g          389 t
ORIGIN      
        1 ctcggctacc agttcaccgt cgactgcgcc gaccccgagg gcaacgagtt ctgcgtgggc
       61 tgacccaggc gtcagtgctg ctcgatgccg ggcggcggcg gaggtgccgc gccgtcccgc
      121 ttgtcggcac gcagcgacgc ccagatggtc acggccagca ccgccgcgat gacccccagg
      181 gagatcgtcg tgggcatgtg gtagacgttc gcgagcagca tcttcacgcc gacgaacgcc
      241 aggatcacgc ccaggcccac gtgcaggtag cggaacttgc ccgccatgcc cgccagcagg
      301 aagtacagcg agcgcaggcc caggatcgcg aacgcgttgg acgcgaacac gatgaagggc
      361 tcccggctga ccgccaggat cgccggcacc gagtccacgg cgaagatgac gtcggtggcc
      421 tccaccagca ccagcacggt gaacagcggc gtggccaggc gcttgccgtt ctccttggtg
      481 aacagcttct ggccgtcgta gtggctggtg gtgggcacca cgcggcgcac cagcttcagg
      541 acggggttct gctccgggtg cacttccgcg cccgagtgcc gggcgatctt cacggccgtg
      601 aacagcagga acgcgccgaa gacgtacagc acccagtcga agcggtcgat cagcgcgacg
      661 ccggcgaaga tgaacatggc gcgcatgacc agcgcgccga agatgcccca gaacagcacc
      721 cggaactggt acgcggcggg caccgcgaag taaccgaaca gcaccgccca gacgaagacg
      781 ttgtcgatcg acaggctctt ctcgatcagg tagccggcca ggtactcccc ggcggcctgc
      841 ccgccctgcc accaggccat gacgcccacg aacgccaggc cgagcgtgat ccagacggcg
      901 ctctcgatgg ccgcctcgcg gaagctgatg acgtggggct tgcggtgcac caggagcagg
      961 tcgaccagca gcatgatggc gatggccgcg gtcagcgcac cccagaccca caggggcacc
     1021 tcgaacgagg cgaactgctg gacgtcggtg ctttgcgtgg cggcgaacag tgcggacaaa
     1081 ggacaagccc tccggacgtc gtgcgtgaag gtctctccgg catcgggccg gtgccaccgg
     1141 gccgcgtcgg gccgtgctgt cgatgggcac ccgagggata ctccccttcg cttgcgcgca
     1201 tcgtacgcgg cgccggtacg gtcacaaacc tggccggccc ggccgcctgg ccgggctcag
     1261 tcggcgagcg cggcctgcac ccggcccagc ttcgcggggt tgcgcaccac gtggatgcgg
     1321 gcgaccttgc cgccggccac gtcgaagccg atgacgttgt cgatgtgatc gccgtcccac
     1381 agcagcagcc cgacggagcc gttcacggtg gcgatctccg cccggtgcgc gggcgtcatg
     1441 tacttcgacg ccaggccgag gaagaagcgc gcgatccgct ccggcccgcg gatcggcttg
     1501 cgcgccgcgt ggaccacccc gccgccatcg gacagcagca gcgcgtcctc ggtgagcgtg
     1561 gccatgagcc cggtgaggtc accggtgagg gtggccatga ggaactgctc caccgccgtg
     1621 cgccggcgtt cgtcgtcgcg gtcgaagcgc gggcgcctgg cctggacgtg ctcgcgggcg
     1681 cggtgggcga gctggcgcac ggccgcctcg ctgcgctcca gcatggcggc cagttcggcg
     1741 tagtcatagc cgaacacctc gcgcagcacg aacacggcgc gctccagcgg tgacaggctc
     1801 tccagcacca ccatcagcgc catcgacagc gactcgacgt cctcggcgcg ctgggcgggg
     1861 tccgtgccgg catccgccgc cggcgacacc agcggctcgg gcagccacgg gccgacgtag
     1921 acctcccgcc gcgcgcgggc ggagcgcaac tcgtccaggc acagccgcgt gaccacggtg
     1981 gtcagccacg cgcgcggctc ggcgaggtcc tccggcgcgc gccggaagcg caggaacgcc
     2041 tcctgcacgg cgtcctcggc cgagccgacc tcgccgagca ggcggtaggc cacgccgaac
     2101 aggtgcgcgc gatggggctc gaacgccgcc gcctggtccc tcacgccacc gcctccatgc
     2161 ccgcgccccg cgccgctccg gcggacagcg ccgtggcggc cagcttcgcc gacgccagcg
     2221 ccgcgtcggc caggatgccg cgccgcccgc accagtctcc caccaccgcg aggccctcca
     2281 cgccgggcac cagcgggccc ggccgtcccg ccaggccgcc ggcggcggcc gccggggtgt
     2341 cgtgcatcac ggtcaggccc ggcaggtagc ggtccccggc cacgtcgtcg cgccacccgg
     2401 gcagcgccag gtccagcgcc gcgtcgatct ccgcccggta cgccggcgac ggctcccgcg
     2461 ggcccaggta gcgcaggacc tgcacgagcg tgcggccctg gggggccacg cctgccgcga
     2521 agtggctgtg cacgttgacg tacaccggct ggtccacgcc cagcacccag acgtgggggc
     2581 tcgatggcac gcgtgcgagg ttgagctcgt aggtcgccgc gcgcaccggc acggcgcggc
     2641 cggcccagcc gccgaccacc cccgcgcccg ggccgtccag cagcctggcc gcggcgcgcg
     2701 ggcctccggc ggcgaccacg gcgccgtcca cggccacgcg cgcgccgtcg gccaggcgca
     2761 ccgcgcgcac gcgctcgtcg tgctcgaccg catcgacgcg gcagtgctcg cgcacgtcca
     2821 cgccggcagc ctgcgccgcc tgccgcagcc cgtcgacgag ctgctgccag ccgccgtgca
     2881 ggtaccacac gccgtgcagc gcgagctgga gctggcggac ggcggcggag gcggcctgcc
     2941 ggtgcgggtc gtgcgtgaac gagctcagcc gcaccagcgc caggatcagc tcccgggcgc
     3001 gctcccgggt cacgtgctgg tccagccacg cggacagcgt cacgtggtcc agggcgcgca
     3061 gccgggcgcc gcgggcgtcg cgggggacgg cggccatggc gcgggccatg tccacgcggt
     3121 ccgcgccgtc cagcagcggc gacagcgccg tgcgcgcgct gaacggcgcg ggcacgatcg
     3181 ccccgccgtg cagcgcctcc atgaacgccg caccgcggat gaccgccccg cgcgggcgca
     3241 cgcccagctc ccgcagcacc ggcaccgcgg gccccttgcg gtacagggcg tgcccgcccc
     3301 agttccacag gtagccgccg tggtcctcgc tggccgccat gccgccgagc gcgcgcccct
     3361 cgaac
//
LOCUS       DATMKC010000191         4092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_246716, whole genome shotgun sequence.
ACCESSION   DATMKC010000191 DATMKC010000000
VERSION     DATMKC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246716"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VNU01_05540"
     CDS             complement(<1..829)
                     /locus_tag="VNU01_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWH32117.1"
                     /translation="MRERIRIFTLLCALLLVAAACGGGDGGDSGDGDGDGGEPVELVW
                     AIGGAEANPGGAHHRTVELWNEANPDTPVRIETLPESADEQRQQMALELDAGGDGFDI
                     LGVDVIWTGEFAENGWLESLEDVRGDLEGEILDGPFESGLWDGELWVAPYNSNAGFLY
                     YRTDLVDTPPTTWEEAKQMAAEHDIDGYVGQGAKYEGFVVNYLEYFWGAGGELLGDGG
                     EVQFGEGDAAQTALEFMTESAQDGFYAPGFNTMKEEEARNHFQAGNAVFMRNWPYAYD
                     "
     gene            complement(842..2032)
                     /gene="ugpC"
                     /locus_tag="VNU01_05545"
     CDS             complement(842..2032)
                     /gene="ugpC"
                     /locus_tag="VNU01_05545"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HWH32118.1"
                     /translation="MAEVRFEEMGKSYPDGTSAVTDFSLTIADGEFVVLVGPSGSGKS
                     TVLRMVAGLESISSGRLLIGDRDVSDLPPRDRDIAMVFQSYALYPHMTVAENMGFALK
                     LAKLPKEEIDARVRDAADILGLTEYLDRKPKALSGGQRQRVAMGRAIVRKPQAFLMDE
                     PLSNLDAKLRVQMRAEIARIQDRLGVSTLYVTHDQVEAMTMGDRVAVLRRGRLMQVAP
                     PQELYDRPDNLFVAGFIGSPAMNIAEATLEREGDELYAELGTFRLDVPFQALEHYPRV
                     RDYAGRKIALGMRPEHFSRIEGERPEGRVVRADVALVEALGAEVVMHFQTDARAVVTA
                     DTREAADTDEVIAELERQAREGQTFCARLDPHHVPKVGEVIELEFDTQHLHFFDLESG
                     TALR"
     gene            2208..3176
                     /locus_tag="VNU01_05550"
     CDS             2208..3176
                     /locus_tag="VNU01_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32119.1"
                     /translation="MALVQTVRGPVDGAELGPTLVHEHVFVLTADVQQNWPGEWGDED
                     ARVDDAVAKLTALAQTGVRTIVDPTVVGLGRFIPRIARIAERVPLQIVVATGIYTYGD
                     VPFFFRSRGRGLHPDLPEPMVDLFVQDLTEGIAGTGVRAAFLKCAVDEHGLTPDVERV
                     LRAVAQAHHVTGAPVMVHTHPATRQGLAVRRVLDEEGVPAGVVMLAHSGDTQDADHLA
                     QLAEAGFVLGMDRFGLETVASFEQRVDTVAELCRRGYAASMGLSHDTACYVDWAEPAA
                     RDWLPRWHYLHIHQDVLPALRERGVTQEQIDAMLVDTPRRWLEAGR"
     gene            3179..>4092
                     /locus_tag="VNU01_05555"
     CDS             3179..>4092
                     /locus_tag="VNU01_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32120.1"
                     /translation="MSETREPASRAPLGVARLALAGFTVLALGAVGLEISRTDVTTTL
                     EPRARRPAAVVTDGRRILEVEARTGRVVSTLLALPTTGEERVVAVAPRPGTFHGLPLV
                     VLSSDPDGPLLWWIDEGGHRRDLPASLARPEVEGGPRSVDPVPVWSPDGSSFAWLEGP
                     HQSPRLRVAAWSADGPREGDERHSGAVLGRGLGPGARLEAWRWDLPGRPGMGTFLISD
                     DQPGLSQVEVRSEDGHVRPLNGRPRRLPGAIVDRADAGRGQAGGPWPRYQLVLPMGEA
                     PQLRWLTGAGASGTLPLPDGLGGSRSRW"
BASE COUNT          533 a         1484 c         1484 g          591 t
ORIGIN      
        1 ggtcgtaggc gtagggccag ttgcgcatga acaccgcgtt gccggcctgg aagtggttgc
       61 gggcctcttc ctccttcatg gtgttgaagc ccggcgcgta gaagccgtcc tgcgcggact
      121 cggtcatgaa ctccagcgcc gtctgcgccg cgtcgccctc gccgaactgc acctcgccgc
      181 cgtcaccgag cagctcgccg ccggcgcccc agaagtactc gaggtagttg acgacgaagc
      241 cctcgtactt ggcgccctgg cccacgtagc cgtcgatgtc gtgctcggcg gccatctgct
      301 tggcctcctc ccacgtggtc ggcggcgtgt cgaccaggtc cgtgcggtag tacaggaagc
      361 cggcgttgga gttgtacggc gccacccaca gctcgccgtc ccacaggccc gactcgaacg
      421 ggccgtcgag gatctcgccc tccaggtcgc cgcggacgtc ctccaggctc tccagccagc
      481 cgttctcggc gaactcaccg gtccagatca cgtcgacgcc gaggatgtcg aagccgtcac
      541 cgccggcgtc gagctccagt gccatctgct ggcgctgctc gtcggccgac tccggcagcg
      601 tctcgatgcg caccggcgtg tcggggttcg cctcgttcca cagctccacc gtgcggtggt
      661 gggcgccgcc ggggttggcc tcggcgccgc cgatcgccca gaccagctcg accggttcgc
      721 cgccgtcgcc gtcaccgtcg ccgctgtcgc cgccatcgcc tccgccgcag gccgccgcga
      781 ccagcagcag cgcgcacagc aacgtgaaga tcctgatccg ttctcgcatg agcctgtctc
      841 cttagcgaag tgccgttccc gactccagat cgaagaagtg caggtgctgg gtgtcgaact
      901 ccagctcgat cacctcaccc accttgggga cgtgatgcgg gtccagccgg gcgcagaagg
      961 tctggccctc gcgggcctgg cgctccagct cggcgatgac ctcgtcggtg tcggcggcct
     1021 cgcgcgtgtc cgctgtcacg accgcgcgcg cgtcggtctg gaagtgcatg accacctccg
     1081 cgcccagcgc ctccaccagc gccacgtcgg cgcggaccac acggccctcc gggcgctcgc
     1141 cctcgatgcg cgagaagtgc tcggggcgca tgcccagggc gatcttgcgg ccggcgtagt
     1201 cccgcacccg cgggtagtgc tccagcgcct ggaacggcac gtccagcctg aacgtgccca
     1261 gctccgcgta cagctcgtcg ccctcgcgct ccagcgtcgc ctcggcgatg ttcatggccg
     1321 gcgagccgat gaagccggcc acgaagaggt tgtccggccg gtcgtagagc tcctgcggcg
     1381 gggcgacctg catgagccgg ccgcggcgca ggacggccac gcggtcgccc atcgtcatcg
     1441 cctcgacctg gtcgtgggtg acgtacagcg tgctgacgcc gagccggtcc tggatgcggg
     1501 cgatctcggc gcgcatctgc acgcgcagct tggcgtccag gttggacagg ggctcgtcca
     1561 tgaggaacgc ctgcggcttg cgcacgatgg cccggcccat ggcgacgcgc tggcgctgcc
     1621 cgccggacag cgccttgggc ttgcggtcga ggtattccgt gaggcccagg atgtccgcgg
     1681 cgtcgcggac gcgggcgtcg atctcctcct tgggcagctt cgccagcttc agggcgaaac
     1741 ccatgttctc tgccacggtc atgtgcgggt acagcgcgta gctctggaac accatggcga
     1801 tgtcgcggtc acgcggcggc aggtcgctga cgtcccggtc gccgatgagc agccgccccg
     1861 aactgatgga ctccaggccg gcgaccatgc gcaggaccgt cgacttgccg gatccggagg
     1921 gacccaccag caccacgaac tccccgtcgg cgatcgtgag cgagaagtcg gtgactgcgc
     1981 tggttccgtc ggggtagctc ttgcccatct cctcgaaacg gacttcagcc acggcgccct
     2041 ccgtactctc cgacagggtt cgtaaccctc gcgtaacgat ccgccggcgc acaagggaag
     2101 gtgatttccg cgaaagcact ttcgtgcctc gccgccgcgc gcatgcgcac ctaccatcgg
     2161 tccccgggcg gttcaggcag ggcagacggc agacggagga gatgcaggtg gcgctggtgc
     2221 agaccgtgcg ggggcccgtc gacggcgccg agctgggccc cacgctggtg cacgagcacg
     2281 tcttcgtgct gaccgccgac gtgcagcaga actggcccgg ggagtggggg gacgaggacg
     2341 cgcgcgtcga cgacgccgtg gccaagctga ccgccctggc ccagaccggt gtccgcacca
     2401 tcgtcgaccc caccgtggtg ggcctcggcc gcttcatccc gcgcatcgcc cggatcgcgg
     2461 agcgcgtgcc gctgcagatc gtggtcgcca ccggcatcta cacctacggc gacgtgccgt
     2521 tcttcttccg cagccggggg cgcgggctgc accccgacct gcccgagccg atggtggacc
     2581 tgttcgtgca ggacctcacg gagggcatcg ccggcaccgg cgtgcgcgcc gccttcctga
     2641 agtgcgcggt ggacgagcac gggctgaccc ccgacgtgga acgcgtgctg cgcgccgtcg
     2701 cgcaggccca ccacgtcacc ggcgcgccgg tcatggtgca cacccacccc gccacccgcc
     2761 aggggctggc ggtgcggcgg gtgctggacg aagagggcgt gcccgccggc gtcgtgatgc
     2821 tcgcgcacag cggcgacacc caggacgccg accacctggc acagcttgcg gaggcgggat
     2881 tcgtgctggg catggaccgc ttcggcctgg agaccgtcgc ctccttcgag cagcgcgtcg
     2941 acacggtcgc ggagctgtgc cgccgcggct atgccgccag catggggctg tcccacgaca
     3001 ccgcgtgcta cgtcgactgg gccgagcccg ccgcgcgcga ctggctgccg cgctggcact
     3061 acctgcacat ccaccaagac gtgctgccgg ccctgcgcga acggggtgtc acccaggagc
     3121 agatcgacgc catgctggtg gacacgccca gacgctggct ggaggcaggc cgctgacgat
     3181 gtccgagacc cgcgagcccg cgagtcgcgc gccgctgggc gtcgcgcgcc tggccctggc
     3241 cgggttcacc gtgctcgcgc tcggcgcggt gggcctggag atctcgcgca cggacgtgac
     3301 cacgacgttg gagccgcgcg cgcggcggcc cgcggccgtg gtcaccgacg ggcgccgcat
     3361 cctggaggtc gaggcccgga ccggacgggt ggtgtccacg ctgctggcgc tgccgaccac
     3421 gggcgaggaa cgcgtcgtgg ccgtggcgcc ccgcccgggg acctttcacg ggcttccgct
     3481 cgtggtcctg agcagcgacc cggacgggcc gctgctgtgg tggatcgacg agggcgggca
     3541 ccggcgggac ctgccggcgt cgctggcgcg cccggaggtg gagggcgggc cgcgctccgt
     3601 ggacccggtg ccggtgtggt cgccggacgg gtcgtcattc gcgtggctgg aggggccgca
     3661 ccagtccccg cgcctgcgcg tggccgcctg gagcgccgac ggcccgcggg agggcgacga
     3721 gcgccacagc ggcgcggtgc tgggccgggg cctgggcccc ggggcacggc tggaggcatg
     3781 gcggtgggac ctgccgggcc ggccgggcat ggggacgttc ctgatcagcg acgaccagcc
     3841 gggcctgtcc caggtcgagg tgcgctccga ggacggtcac gtgcgcccgc tgaacggccg
     3901 cccccggcgg ctgccggggg cgatcgtgga ccgcgcggac gcgggacgag gccaggccgg
     3961 cggcccgtgg ccccggtacc agctggtgct gccgatgggc gaggcgccgc agctgcgctg
     4021 gctcaccggc gcaggcgcca gcggcacact gccgctgccg gacgggctgg gcggcagccg
     4081 gagccggtgg tg
//
LOCUS       DATMKC010000192         7210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_26764, whole genome shotgun sequence.
ACCESSION   DATMKC010000192 DATMKC010000000
VERSION     DATMKC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26764"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="VNU01_05560"
     CDS             complement(<1..1534)
                     /locus_tag="VNU01_05560"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWH32121.1"
                     /translation="MFRAVLVANRGEIALRVLRGCKELGIPTVGVYSEADRGAPWLRL
                     ADQTYLLGPAAPSESYLNVERILEVAQESGAEAIHPGYGFLSENADFARAVQDARLVW
                     VGPPADAIVQMGDKLSARKAAEAAKVPLVPGMLEPTDDPEVVRAFGEEHGYPIAIKAA
                     FGGGGRGLKVVRDPKELEDALESAQREALGAFGRAEVYVERYLTRPRHIEAQILADQH
                     GNTLFLGERDCSSQRRHQKLIEEAPAPHFPEDVRKAFGEASAKVAREVGYVGAGTVEY
                     LYEDGEFFFLEMNTRLQVEHPVTELITGMDLVHWQLRIAAGEELPFRQEDIELRGHAI
                     ELRINAENVAMGFVPSPGLITRWEIPSGPGVRIDAGVETGWEIPRTYDSLIAKLIGYG
                     ATRDEARRKLIRALDELVIEGVPTTADFHRFALEHPDFIEGNVSTVTVEKEWDLSGIP
                     AAEPPAAGDGPQEPSQTIAVEVGGKRLDVKLFGLEGVSLGGGGGAGAKAAPRKRGGSG
                     GKG"
     gene            1767..2210
                     /locus_tag="VNU01_05565"
     CDS             1767..2210
                     /locus_tag="VNU01_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWH32122.1"
                     /translation="MSRKRLALFAALTALVLVMAGCSKEEPAFTAEDQIPEDLVATED
                     EGGGEGEDGAAGGGETLHFTAADIKFADAPATAPAGTVTIELVNEGGSPHDVTFEELG
                     DDPVVVAQGGETATGTAELEPGTYTYYCSVPGHRSAGMEGELTVE"
     gene            complement(2291..2524)
                     /locus_tag="VNU01_05570"
     CDS             complement(2291..2524)
                     /locus_tag="VNU01_05570"
                     /inference="COORDINATES: protein motif:HMM:NF025192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HWH32123.1"
                     /translation="MSEASDEAATGPRLRIEGQATPEEVAAVTAALAAVLSSAGEPEP
                     EHQSGWVIAARLEAQGHPQVSTLRHIAAHATTH"
     gene            complement(2521..4077)
                     /locus_tag="VNU01_05575"
     CDS             complement(2521..4077)
                     /locus_tag="VNU01_05575"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWH32124.1"
                     /translation="MADTTKQKLEEFQRRVYEAEHAGSERAIEKQHAKGKRTARERID
                     LLLDEGSFVETDRLAVHRAYGFGLEDKRIPGDGVVTGWGTIDGRRVCVFSQDFTVFGG
                     SLGEVFAEKIHKVMDLALTMGVPIIGINDSGGARIQEGVVSLAQYGEIFMRNVKSSGV
                     IPQISAIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPEVIKTVTGEDVSFEELGGA
                     MSHMAKSGVTHFAADDEEACVEDIKYLLSFLPSNNLEEPPRVATGDDPQRLDLDLDTF
                     MPDSSNVPYDMREIIRRVVDDEEFFEVQPHYAENIVCGFARLDGYAVGVVGNQPMHLA
                     GTLDMTSSEKAGRFVRTCDAFNVPIVTFVDVPGFMPGTTQEWGGIIRRGAKLLYAYCE
                     ATVPKLTVITRKAYGGAYVVMGSKHAGADINFAWPTAEIAVMGANGAANILWGKEIAA
                     AEDPEAKRAELIEHYEHELANPWKAAERGYVDDVIEPAETRKRLIQALHLTRTKRQAR
                     PPRKHGNIPL"
     gene            4138..4938
                     /locus_tag="VNU01_05580"
     CDS             4138..4938
                     /locus_tag="VNU01_05580"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWH32125.1"
                     /translation="MSAEESSQAGPPPQLVRTLVEGPAPVASLEWHAEVDSTNVRAAE
                     LARAGAPEIAVVGAEHQSAGRGRMGRVWSAPPRSSLTFSLLLRPPVAAEALPLLPLLA
                     GLALAEVAGRYCTQVEVGLKWPNDLLIDGRKAAGVLVESAEAGAVVVGIGLNTDWRGI
                     ERWDELADAISLAEASQFPIDRWRVLAALLGVFAQRYEVFKDLPAAFLDGYRARCLTI
                     GRAVRVERVAGEPLLGEAVGVDASGALEVRDAAGTLHRLSAGDVHHLR"
     gene            complement(5009..5866)
                     /locus_tag="VNU01_05585"
     CDS             complement(5009..5866)
                     /locus_tag="VNU01_05585"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HWH32126.1"
                     /translation="MRRALILSLTLLLGLAACGDGGGGTEPTPSPTTASPSPTESETE
                     ATQESTAWFVRAADGWMWVEPETHLVPQTEGVARAAMEVLFAGETDDPNLDSMVEGVE
                     VLGADIDGDVLTVDVSGDIDPDGAGRGSAEEIAFAQQLAYTAAQFDGVRAVRLLVDGE
                     EITDLWGHLDWSQPIEPDPFAETPITIDEPGWGAEVPQRALTASGEANTFEATLAVRL
                     IDPDGAVAEEEWITATSGSGTRGTWEHTFSSELDRPGTWAVEAEEPDPSGGEEGRPPL
                     VVRSEFTVT"
     gene            complement(5931..6782)
                     /locus_tag="VNU01_05590"
     CDS             complement(5931..6782)
                     /locus_tag="VNU01_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWH32127.1"
                     /translation="MRRALAVPALAALLALTLVATSYGIATYSTVFRRAYLKDGLLTI
                     LVIGSDLGPPHRPANPLRGRADGVHLIAVDTNAKRATIVDFPRDSMIGGRKVNGHLAA
                     GGPSRLEGAMEGYTGIPIDFWVLATFRSVENVVDGMGGVDVVVETRMRDAFSGSDFEP
                     GPQRLAGWQALTFVRDRKSVPGGDFGRTRNQGSLLRYAHAQIRRSHADLPELVRLAGL
                     LARNTATNIPKTELVQLAVLAAEIPAANVLQVPLSGSAGLERGASVVRLAPGGTFDRI
                     RAGQVGP"
     gene            complement(6782..>7210)
                     /locus_tag="VNU01_05595"
     CDS             complement(6782..>7210)
                     /locus_tag="VNU01_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32128.1"
                     /translation="QFVDPATRFTSGPARALLRDGVFDGLPPEQRAAMGVQYQPVAGV
                     VPGAATGEATVLFEQGRAYAVSIDYRLELELHYPRGPETATPAEQQEPVELRQPLSHS
                     GTVLFTAPSWAVESFEFRTKAPDVPVPPPAPSPTTGEVAS"
BASE COUNT          920 a         2610 c         2615 g         1065 t
ORIGIN      
        1 cgcccttgcc gccggagccg ccgcgcttgc gcggcgccgc cttcgccccg gccccgccgc
       61 ctccgcccag tgagacgccc tccaggccga acagcttgac gtccaggcgc ttgccgccca
      121 cctccacggc gatggtctgg ctcggctcct gcgggccgtc gccggccgcg ggcggctcgg
      181 ccgccgggat gccggacagg tcccactcct tctccaccgt gacggtggag acgttgcctt
      241 cgatgaagtc ggggtgctcc agggcgaagc ggtggaagtc ggccgtggtc ggcacgccct
      301 cgatcaccag ctcgtccagc gcgcggatca gcttgcggcg cgcctcgtcg cgcgtggcgc
      361 cgtagccgat cagcttggcg atcagcgagt cgtacgtgcg cgggatctcc cagcccgtct
      421 ccacgcccgc gtcgatgcgc acgccgggcc cggacgggat ctcccacctg gtgatcaggc
      481 ccggcgaggg cacgaagccc atcgcgacgt tctcggcgtt gatgcgcagc tcgatcgcgt
      541 ggccgcgcag ctcgatgtcc tcctggcgga acggcagctc ctcgccggcg gcgatgcgca
      601 gctgccagtg caccaggtcc atgccggtga tgagctcggt gaccgggtgc tccacctgca
      661 ggcgcgtgtt catctccagg aagaagaact cgccgtcctc gtacaggtac tccacggtgc
      721 cggcgcccac gtagccgacc tcgcgggcga ccttggcgga cgcctcgccg aacgccttgc
      781 gcacgtcctc cgggaagtgc ggggcgggcg cctcctcgat gagcttctgg tggcggcgct
      841 ggctggagca gtcacgctcg cccaggaaca gcgtgttgcc gtgctggtcg gccaggatct
      901 gcgcctcgat gtggcgcggg cgcgtgaggt agcgctccac gtacacctcg gcgcggccga
      961 aggcgcccag ggcctcccgc tgcgccgact ccagcgcgtc ctccagctcc ttggggtcgc
     1021 gcacgacctt gagtccccgg ccgccgccgc cgaacgcggc cttgatggcg atggggtagc
     1081 cgtgctcctc accgaaggcc cggacgacct ccgggtcgtc ggtcggctcc agcatgccgg
     1141 gcaccagcgg gaccttggcc gcctcggcgg ccttgcgcgc cgacagcttg tcgcccatct
     1201 gcacgatcgc gtccgcgggc gggccgaccc agaccagcct cgcgtcctgc acggcgcggg
     1261 cgaagtcggc gttctccgac aggaagccgt agcccgggtg gatcgcctcg gcgccggact
     1321 cctgcgcgac ctcgaggatg cgctcgacgt tgaggtagct ctccgacggg gccgccggcc
     1381 ccagcaggta ggtctggtcg gccaggcgca gccacggcgc cccgcggtcg gcctccgagt
     1441 acacgcccac cgtggggatg cccagctctt tgcagccacg caggacacgc agggcgatct
     1501 cgcctcggtt ggccaccagc accgcgcgga acattgcacc gtccttgttc aggggcgggt
     1561 cgcccggatc ggggcccgag tcaggccggg ggcgacgggt cgtcgggcgt gatgctatcc
     1621 attccgcgcc gcggcaccga atgcgggtct tccggcccgg tttcgccggg acggttgcgg
     1681 cgcggtcgtt ttgccgaccc accgcgccga gaggacactg gggaggcacc gccccctcat
     1741 cgcgtcgtcg cacaaggagt gcattcatgt cgcgcaagcg tctcgcgctg ttcgccgccc
     1801 tgaccgccct ggtcctggtc atggccggct gctccaagga ggagcccgcg ttcaccgcgg
     1861 aggaccagat cccggaggac ctggtcgcca cggaggacga gggcggaggg gaaggggagg
     1921 acggcgccgc cggcggcggc gagacccttc acttcacggc tgcggacatc aagttcgccg
     1981 acgcgccggc gaccgcgccg gccggcaccg tcacgatcga gctggtgaac gagggcggct
     2041 ccccccacga cgtcacgttc gaggaactgg gcgacgaccc ggtcgtggtc gcacagggcg
     2101 gcgagaccgc cacgggcacc gcggagctgg agccgggcac ctacacctac tactgctccg
     2161 tgccgggcca ccgctccgcc ggcatggaag gcgagctcac cgtcgagtag ccctcagcac
     2221 gtcaaatggg tgcccccact gcggtatctc gcagtggggg cacccatttg cagcgggaac
     2281 agcgccgtcg tcagtgcgtg gtggcgtggg cggcgatgtg ccgcagggtg gagacctgcg
     2341 gatggccctg cgcctccagc cgcgcggcga tcacccagcc ggactggtgc tccggctccg
     2401 gctccccggc cgacgacagc accgccgcca gcgccgcggt gaccgcggcg acctcctcgg
     2461 gcgtggcctg gccctcgatg cgcagccggg ggccggtcgc cgcctcgtcg gacgcctcgc
     2521 tcacagcggg atgttcccgt gcttgcgcgg cggccgcgcc tggcgcttgg tgcgcgtcag
     2581 gtgcagcgcc tggatgaggc gcttgcgcgt ctcggccggc tcgatgacgt cgtcgacgta
     2641 gccgcgctcg gcggccttcc acgggttggc cagctcgtgc tcgtagtgct cgatgagctc
     2701 ggcgcgcttg gcctcgggat cctcggccgc cgcgatctcc ttgccccaca gaatgttggc
     2761 cgcgccgttg gcgcccatga cggcgatctc cgcggtcggc caggcgaagt tgatgtcggc
     2821 gccggcgtgc ttggagccca tgaccacgta cgcgccgccg taggccttgc gggtgatgac
     2881 cgtgagcttg ggcacggtcg cctcgcagta ggcgtacagc agcttggcgc cgcggcggat
     2941 gatgccgccc cactcctgcg tggtgcccgg catgaagccc ggcacgtcca cgaacgtgac
     3001 gatcggtacg ttgaacgcgt cgcacgtgcg cacgaaccgg ccggccttct cggagctggt
     3061 catgtccagc gtgcccgcca ggtgcatggg ctggttgccg acgacgccga cggcgtagcc
     3121 gtccaggcgc gcgaacccgc acacgatgtt ctcggcgtag tggggctgga cctcgaagaa
     3181 ctcctcgtcg tccacgacgc ggcggatgat ctcgcgcatg tcgtagggga cgttggagct
     3241 gtccggcatg aacgtgtcga gatcgaggtc cagccgctgc gggtcgtcgc ccgtggccac
     3301 ccgcggcggc tcctccaggt tgttggaggg caggaagctc agcaggtact tgatgtcctc
     3361 gacgcaggcc tcttcgtcgt cggccgcgaa gtgcgtgacg cccgacttgg ccatgtgcga
     3421 catcgcgccg ccgagctcct cgaagctcac gtcctcgccg gtgacggtct tgatgacctc
     3481 gggaccggtg atgaacatgt gcgaggtctc cttcaccatg aagatgaagt cggtcatcgc
     3541 gggggagtac acggcgccgc cggcgcaggg gcccatgatc gcgctgatct gggggatgac
     3601 gccggaggac ttcacgttgc gcatgaagat ctcgccgtac tgggccagcg agaccacgcc
     3661 ctcctggatg cgcgccccac cggagtcgtt gatgccgatg atcggcacgc ccatcgtcag
     3721 cgccaggtcc atgaccttgt ggatcttctc ggcgaagacc tcgcccaggc tgccgccgaa
     3781 gacggtgaag tcctggctga acacgcacac gcggcggccg tcgatggtgc cccagccggt
     3841 gaccacgccg tcgccgggga tgcgcttgtc ctccaggccg aagccgtacg cccggtgcac
     3901 cgccaggcgg tcggtctcca cgaaggagcc ttcgtccagc agcaggtcga tgcgctcgcg
     3961 cgcggtccgc ttgcccttgg cgtgctgctt ctcgatggcg cgctccgagc ccgcgtgctc
     4021 ggcctcgtac acgcgccgct ggaactcttc gagcttctgc ttcgtggtgt cggccacgcc
     4081 tcgctccact gggtccatcc ggtccgtcgg gggtcgcgcg gattgtaggg tcccgccatg
     4141 agcgccgaag aatcgtccca ggccgggccg ccgccccagc tggtccgcac gctggtcgag
     4201 ggtcccgcgc cggtcgcctc gctggagtgg cacgccgagg tggactccac caacgtgcgc
     4261 gccgccgagc tggcgcgcgc cggggcgccc gagatcgccg tggtgggggc ggagcaccag
     4321 agcgccgggc gcggccggat gggccgggtc tggagcgcgc cgccgcgcag ctccctgacg
     4381 ttctcgctgc tgctgcgccc gccggtggcg gccgaggcgc tgccgttgct gccgctgctg
     4441 gccggcctgg ccctggccga ggtggccggc cgctactgca cgcaggtgga ggtcggcctc
     4501 aagtggccca acgacctgct gatcgacggg cgcaaggccg ccggcgtgct ggtggagtcg
     4561 gcggaggccg gggcggtggt ggtcggcatc gggctcaaca cggactggcg cggcatcgag
     4621 cgctgggacg agctggccga cgcgatctcg ctggccgagg cctcgcagtt cccgatcgac
     4681 cgctggcgcg tgctcgcggc gctgctcggc gtgttcgccc agcgctacga ggtgttcaag
     4741 gacctgccgg ccgcgttcct ggacggctat cgcgcccggt gcctgacgat cgggcgggcc
     4801 gtgcgcgtgg agcgcgtggc gggggagccg ctgctgggcg aggcggtcgg tgtcgacgcc
     4861 tcgggggcgc tggaggtgcg cgacgccgcc gggacgctgc accgcctgtc cgccggcgac
     4921 gtccaccacc tccgctgacg gccccgcccc tgacccccga gttggaagat gaggcccgta
     4981 tatgggccag atcttccaac tcggggggtt aggtgacggt gaactcgctg cggaccacca
     5041 gcggggggcg gccttcctcg ccgccggacg ggtccggctc ctccgcctcc accgcccagg
     5101 tcccgggccg gtcgagttcc gaggagaagg tgtgctccca ggtgccacgc gtgccgctgc
     5161 cggacgtcgc cgtgatccac tcctcctcgg ccaccgcgcc gtcggggtcg atcaggcgca
     5221 cggccagcgt cgcctcgaac gtgttggcct cgccggacgc ggtgagcgcg cgttggggga
     5281 cctcggcgcc ccagcccggc tcgtcgatgg tgatcggcgt ctcggcgaac gggtcgggct
     5341 cgatgggctg cgaccagtcg aggtggcccc acaggtccgt gatctcctcg ccgtcgacca
     5401 gcaggcgcac cgcgcggacg ccgtcgaact gcgcggccgt gtaggcgagc tgctgcgcga
     5461 acgcgatctc ctcggcggag ccgcgaccgg caccgtcagg gtcgatgtcg ccggagacat
     5521 ccaccgtcag cacgtcaccg tcgatgtccg cgcccagcac ctccacgccc tccaccatgc
     5581 tgtccaggtt cgggtcatcg gtctcgcccg cgaacagcac ctccatcgcg gcgcgggcca
     5641 caccctcggt ctgcggcacc aggtgcgtct ccggctccac ccacatccag ccgtcggcgg
     5701 cgcgcacgaa ccaggcggtg gactcctggg tcgcctcggt ctcggactcc gtcggcgacg
     5761 gagaggccgt cgtgggcgac ggggtgggct ccgtgccgcc gccgccgtcg ccgcacgcgg
     5821 ccaggcccag cagcagggtc agcgaaagga tcagcgcgcg gcgcatgggg cctccggtcg
     5881 tgtgcggtca cctgctcaga cgctcagcct gcccccgggg ttgcgcgttg tcaggggccg
     5941 acctgccccg cgcggatgcg gtcgaaggtc cccccgggcg ccaggcgcac cacggaagcg
     6001 ccgcgctcca acccggcgct gccgctgagc ggcacctgca ggacgttggc ggcggggatc
     6061 tccgcggcca gcaccgcgag ctgcaccagc tccgtcttgg ggatgttggt cgccgtgttg
     6121 cgggccagca gcccggccag ccgcaccagt tccggcaggt cggcgtgcga gcggcggatc
     6181 tgggcgtggg cgtagcgcag cagcgagccc tggttgcgcg tccgcccgaa gtccccgccg
     6241 ggcacggact tgcggtcgcg cacgaacgtc agcgcctgcc accccgccag ccgctgcggg
     6301 cccggctcga agtccgagcc ggagaacgcg tcgcgcatgc gcgtctccac cacgacgtcc
     6361 acgccgccca tgccgtcgac gacgttctcg acggagcgga aggtggccag gacccagaag
     6421 tcgatgggga tcccggtgta gccctccatg gcgccttcga ggcgtgacgg gccgcccgcc
     6481 gcgaggtggc cgttcacctt gcgcccgccg atcatcgagt cccgcgggaa gtcgacgatg
     6541 gtggcgcgct tggcgttggt gtccacggcg atcaggtgca cgccgtccgc acgaccgcgc
     6601 agcgggttgg cgggccggtg cggcgggccc aggtcggagc cgatcaccag gatggtgagc
     6661 aggccgtcct tcaggtaggc gcggcggaac accgtggagt acgtcgcgat gccgtagctg
     6721 gtggccacca gcgtcagcgc cagcagggcc gccagggccg gcacggccag tgcgcggcgc
     6781 atcacgacgc cacctccccc gtggttggtg acggcgcggg cggcggcacc ggcacgtcgg
     6841 gcgccttggt gcggaactcg aaggactcca cggcccagct cggtgcggtg aacagcaccg
     6901 tgccgctgtg cgacagcggc tggcgcagct ccacgggctc ctgctgctcg gctggcgtcg
     6961 ccgtctccgg cccgcgcgga tagtgcagct ccagctccag gcggtagtcg atggagacgg
     7021 cgtaggcgcg gccctgctcg aacagcacgg tggcctcgcc cgtggcggca cccggcacca
     7081 cgccggccac cggctggtac tgcacgccca tcgccgcgcg ctgctccggc ggcaggccgt
     7141 cgaacacgcc gtcgcgcagc agtgccctgg ccggcccgga ggtgaaccgc gtcgccgggt
     7201 ccacgaactg
//
LOCUS       DATMKC010000193         8701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_160349, whole genome shotgun sequence.
ACCESSION   DATMKC010000193 DATMKC010000000
VERSION     DATMKC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160349"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..643)
                     /locus_tag="VNU01_05600"
     CDS             complement(80..643)
                     /locus_tag="VNU01_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32129.1"
                     /translation="MDDNSQAGPTRRRIDRITAPDYLDGIETRDAAELRAMRDDCGEE
                     ESRFSYNRRLIQGRIDIIGAELERRAGNSSESLIDALPRILADDPGPRSGAGNARNSP
                     VYEPGEVGRRSDDRLAADALLSRLPDLTDTELQAELEELREEERRVSELRRAVQERFD
                     ALQAELIRRYREGGVDDIVSAMPGDAD"
     gene            complement(741..1835)
                     /locus_tag="VNU01_05605"
     CDS             complement(741..1835)
                     /locus_tag="VNU01_05605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH32130.1"
                     /translation="MKGRVVTVAWRRIGAVALSACLAVALLPALASGGTHDAAVGQAQ
                     ERLRSAQAREQALATALDQMAESFERAQAHHLRLQEELVGVERSVDRVRTAQEAARES
                     LRRRVRAAYRAPHTDLALASAVLDAPDAATAMHRAGLMARLTVTAAHEMADVRQDEAR
                     AVAALRQRRIIEVGARGAAQAQRAEAERLVAALQQAARSVGEAERELEHARERARAAA
                     RAAELSAAVVLPAVDGKVCPVAGPHGFIDSWGFPRSGGRRHQGVDMFAEYGTPLAAVA
                     DGTVRRVYSNRLGGLSVDLIDRDGNRYYYAHLASASVSDGQQVAAGDVVGAVGDSGNA
                     RGTPPHLHWQFHPGGGAPVNPYPLARTLCR"
     gene            complement(2044..3222)
                     /locus_tag="VNU01_05610"
     CDS             complement(2044..3222)
                     /locus_tag="VNU01_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32131.1"
                     /translation="MSHVIDPPPLELDWELYRPRHDTLRAEVRAWVDASIRPHVDEWE
                     AQDEPGFPRGLFADAARAGLFGWKVDEAFGGNGVDFLADCVVVEELARGGSGGVAAAL
                     GAHKDLGSYYIWRFGTDEQRARWVPPSMHGEIVTALGVTEPHAGTDVARIRTRAVRQP
                     GGDWLLNGTKTFITNGSWADVLVIAAQTTEEGGHHGMTLFVVDKDDPGFTSRRIDTLG
                     WRTSHTGELSFADVRLPADRLLGGEELLHQGFVCIMRNFQWERISMALAGTVAARRVL
                     DEGVRLLVDRHGRIPEGWRWRLADLDAEVRAVRALTVHALRLYLHGQDAVREVSMAKA
                     KASVLSVTVADAVLEAHGASGVTATPWIQRALRDSRLNPIGGGTTEIMLEVVGRTLGL
                     "
     gene            complement(3234..3749)
                     /locus_tag="VNU01_05615"
     CDS             complement(3234..3749)
                     /locus_tag="VNU01_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWH32132.1"
                     /translation="MKSDSTVVDVFVEIPTGSRNKYEWDHDAGRFVLDRMLFSAMHYP
                     GDYGFVHDCWGEDGDPLDAVVILGEPTFPGCTIRTRVVGYLRMRDEKGEDAKILGVPD
                     TDPRWSHVHELEDLPGHLLEEVEHFFARYKDLEHKVVQLDGWGGREEAERIIAEGFER
                     WAELDPKPAMP"
     gene            complement(3746..4840)
                     /locus_tag="VNU01_05620"
     CDS             complement(3746..4840)
                     /locus_tag="VNU01_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32133.1"
                     /translation="MSTSLFTEEHEALRAVVRRWVEREGAEGAPAWERTGGWPRDVLR
                     TVASQGWLSTALAASGGGDVLAGVAMWEELGRLRSAGVVQDLLAQAHAAALLSEAGVP
                     AVAQRAHAGELLVTLAGGALRLRPDGDALLLSGTAPALANAAVADTAVAVVPDEQGIA
                     LAVVDTRLPGWHATPPASPFGRRGGHAADVEFRDVRLAGGDVVARGAEGLALMRRRQD
                     RWRLDHAAATVAAAWLAWEDAKAYALQREAFGRPIATFQVNRHALADMAAELSALRAL
                     VHDTARRLTLAALESGDTAALRLRADRVSAAVADRCLQLHGGYGYTMEFDVQRAWRDA
                     AHLRVDEAGDAALRDEIAAGLESRMGGGSS"
     gene            4897..5127
                     /locus_tag="VNU01_05625"
     CDS             4897..5127
                     /locus_tag="VNU01_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32134.1"
                     /translation="MSTPLPSPAEPVPLPDPGEPTPLPDPGRPVPVPDPGEPTPVPEP
                     DEPMPLPGPAEPQPLAAAHQPSRSRRAATLAA"
     gene            complement(5082..5876)
                     /locus_tag="VNU01_05630"
     CDS             complement(5082..5876)
                     /locus_tag="VNU01_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HWH32135.1"
                     /translation="MRPELPPDLGEPETWRRVPGGDICESWEAAWADGRRAFVKRTPY
                     PARIEADGLEALRRAGAPVPGVLGVAPHTLVLEWLEGSSEWAGLGRALATVHRATAKA
                     FGWPEDNRLGSLVQRNGDWPQWAEFYAERRVRPFLSAGGLDDALRRRLRAALDGPLQD
                     LLGAHAPAPSLVHGDLWAGNVVDGRWLVDPAVCFADRELDLAFAEVFGGFPEAFHHAY
                     QEAWPLPDGWRERRPALQLYHLLAHVKLFGGGYAASVAARLDRLGW"
     gene            complement(5873..6346)
                     /locus_tag="VNU01_05635"
     CDS             complement(5873..6346)
                     /locus_tag="VNU01_05635"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HWH32136.1"
                     /translation="MRILVVCLGNICRSPTAEAALREALRDAGLDGQVEVASAGTGDW
                     HLGEPPDARMTRAAAAAGLTLEGTAAQITAEDFRRSDLILVMDRSNLRDVRALAPDEP
                     SRSKVRLYRDFDPDADHDEVPDPYYGGEDGFHAVVDIARRTARGVVEHVRGLLTA"
     gene            6402..6866
                     /gene="bcp"
                     /locus_tag="VNU01_05640"
     CDS             6402..6866
                     /gene="bcp"
                     /locus_tag="VNU01_05640"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HWH32137.1"
                     /translation="MGLKPGDTAPDFTLLDQDGTPWTLAEQRGRPVVLYFYPKDDTPG
                     CTTQGCDIRDHWSRFEAAGVVVAGVSPDSAESHAKFAGKYGFPHTLLADTARTVIQSY
                     GAWGERSMYGKKYMGVVRSSVVIDAAGKVAAVFSPIKPADQSKKALEALAAL"
     gene            complement(6912..7796)
                     /locus_tag="VNU01_05645"
     CDS             complement(6912..7796)
                     /locus_tag="VNU01_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32138.1"
                     /translation="MALLLDAPAFVRRPAGVVRVTGEDRLSFLHTLLSQHLEDMPAGR
                     AADFLYLDAKAAPVAAGRALVRDADVLLVTPPDVAPALAERLDGMRFLLRVEVADESA
                     DWAMASLRGGAPAGPAEPMTFQEAGGGIVVRDRSGGVDLLGRAAWLDARLAGEALPEA
                     SAEHWEAWRIARGEPAWGREIAPGHRPQELGLLPTHVHLRKGCYPGQESIAKTWNLGR
                     PRRALAVVELDAEPEAGVLRVGDKEAKVTSAAPRDGGWLAMALLPVDPEGRPPETVDV
                     PPGRVLRKVGDGLPIPGA"
     gene            8225..>8701
                     /locus_tag="VNU01_05650"
     CDS             8225..>8701
                     /locus_tag="VNU01_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWH32139.1"
                     /translation="MERIDLPDASATPVVYRDALLEVLGGRDALTVMAETLEALPRLL
                     DGLSDAEWAAAPEPGEWSPAELLGHFFDVDLVFGFRWRLVATSDRPTYPGYDEKAFMG
                     LARPEPARLLTAWRGLREANLAMLRTLPAEAYARLGVHGEQGEETLRVAVDKFAGHD"
BASE COUNT         1127 a         3261 c         3218 g         1095 t
ORIGIN      
        1 gctcacacac gtgcggaaag ttgcccagag gcggcgacaa ccgcatggcg cgaggggacg
       61 ttgcgggcga gggagcggcc tagtcggcgt cgcccggcat cgccgagacg atgtcgtcca
      121 cgccgccctc gcggtagcgg cggatcagct cggcctggag cgcgtcgaag cgctcctgga
      181 cggcgcggcg gagctcggag acgcggcgct cctcctcgcg gagctcttcc agctcggctt
      241 gcagctccgt gtcggtgagg tcgggcagcc gcgacagcag cgcgtcggcg gcgaggcggt
      301 cgtcgctgcg gcggccgacc tcgccgggct cgtacaccgg ggagttgcgg gcgttgccgg
      361 cgccggagcg cggaccgggg tcgtcggcca ggatgcgcgg gagcgcgtcg atcaacgact
      421 cgctgctgtt gcccgcccgg cgctccagct cggcgccgat gatgtcgatg cgcccctgga
      481 tcaggcggcg gttgtaggag aagcgggact cctcctcacc gcagtcgtcg cgcatggcgc
      541 gcaactcggc cgcgtcgcgc gtctcgatgc catcgaggta gtccggcgcg gtgatccggt
      601 cgatgcgccg gcgcgtcggc cctgcctgcg agttgtcgtc catgcccggc ctccatgtgg
      661 ccccgtcggt gcgggcgcgc gcggcgtgcc cgaggcgccc gcccctccgc gagttcgcag
      721 cagtctacgc ggcggcgtcc tcagcggcag agcgttcgcg cgagcggata gggattgacc
      781 ggcgcgccgc caccggggtg gaactgccag tgcaggtggg gcggcgtgcc gcgcgcgttg
      841 cccgagtcgc cgacggcgcc caccacgtcg ccggccgcca cctgctgccc gtccgagacg
      901 gacgcggacg ccaggtgcgc gtagtagtac cggttgccgt cgcggtcgat gagatcgacc
      961 gacaggccgc ccaagcggtt gctgtacacg cgccgcaccg tgccatccgc gacggccgcc
     1021 agcggcgtgc cgtactcggc gaacatgtcg acgccctggt ggcgccggcc gccggagcgg
     1081 gggaagcccc aggagtcgat gaagccgtgc ggccccgcga cggggcacac cttgccgtcg
     1141 acggcgggca ggaccacggc ggccgacagt tccgcggccc gcgccgctgc gcgggcgcgc
     1201 tcccgcgcgt gctcgagttc gcgttccgct tccccgacag agcgcgccgc ctgctggagc
     1261 gcggcgacga ggcgttcggc ctcggcccgc tgcgcctgcg cggcgccgcg ggcgcccacc
     1321 tcgatgatgc gccgctgccg caaggcggcc accgcgcgtg cctcgtcctg gcgcacgtcc
     1381 gccatttcgt gcgcggcggt gacggtgagc ctcgccatga gcccggcgcg gtgcatggcg
     1441 gtggcggcgt ccggcgcgtc cagcaccgcc gaggcgagcg ccaggtcggt gtgcggcgcg
     1501 cggtacgccg cccgcacgcg gcgtcgcagc gactcccgtg ccgcctcctg cgccgtcctg
     1561 acgcggtcca cggaccgctc cacgccgacg agctcttcct ggaggcgcag gtggtgcgcc
     1621 tgcgcccgct cgaacgactc ggccatttgg tccagcgcgg tggccagcgc ctgctcgcgc
     1681 gcctgcgccg aacgcaggcg ctcctgggcc tggcccaccg cggcgtcgtg cgtgccgccg
     1741 gaggccagcg ccggcagcag ggcgacggcc aggcacgccg acagcgcgac cgcgccaatg
     1801 cgccgccacg ccaccgtgac cacccgaccc ttcacaggtg cctacatcgg ccgaaccctc
     1861 ggccggctga agcggccacg gtggacaggg agggccacgg agggttccgg ccgacaggca
     1921 acggagcggc cacgcgggtt ccggccgagc ggtaaagagg gtttcccccc gaggcggggt
     1981 tccgcggggc cgcgacgcac cgaggccggg tccctccgtg gccctcccgg gagccgatga
     2041 ggactagagc ccgagggtcc ggccgacgac ctcgagcatg atctccgtcg tcccgccgcc
     2101 gatggggttg agccgcgagt cgcgcagcgc ccgctggatc cacggggtcg cggtgactcc
     2161 ggatgcgccg tgcgcctcca ggacggcatc ggcgacggtg accgacagca cggacgcctt
     2221 cgccttggcc atggacacct cgcgcacggc gtcctggccg tgcaggtaga gccgcagcgc
     2281 gtgcacggtg agcgcccgca ccgcgcgcac ctccgcgtca aggtccgcca gccgccagcg
     2341 ccagccttcc gggatgcggc cgtggcggtc caccaggagg cggacgccct cgtccaggac
     2401 gcgccgggct gcgacggtgc cggccagcgc catggagatc cgctcccact ggaagttgcg
     2461 catgatgcag acgaagccct ggtgcagcag ttcctcgccg ccgagcaggc ggtcggcggg
     2521 caggcgcacg tcggcgaagc tgagctcccc ggtgtggctg gtccgccagc cgagcgtgtc
     2581 gatccgccgg gaggtgaagc cggggtcgtc cttgtccacc acgaacagcg tcatgccgtg
     2641 gtgcccgccc tcctcggtgg tctgcgcggc gatcaccagg acgtcggccc aggagccgtt
     2701 ggtgatgaac gtcttggtgc cgttgaggag ccagtcgccg ccgggctggc gcacggcgcg
     2761 cgtgcggatg cgagcgacgt cggtgccggc gtgcggctcg gtcacgccga gcgcggtgac
     2821 gatctcgccg tgcatggacg gcggcaccca gcgcgcgcgc tgctcgtccg tgccgaagcg
     2881 ccagatgtaa taggagccga ggtccttgtg ggcgccgagg gccgcggcga cgccgccgga
     2941 gccgccgcgc gcgagctcct cgaccaccac gcagtccgcc aggaagtcca cgccgttgcc
     3001 gccgaaggcc tcgtccacct tccagccgaa caggccggcg cgcgcggcgt cggcgaacag
     3061 gccgcggggg aaccctggct catcctgcgc ctcccactcg tccacgtggg gccggatgct
     3121 cgcgtccacc caggcgcgca cctcggcgcg cagcgtgtcg tggcggggcc tgtacagctc
     3181 ccagtcgagc tccagcggcg gcgggtcgat gacgtggctc acggtcgcac ctcctacggc
     3241 atcgccggct tggggtccag ctccgcccag cgctcgaagc cctcggcgat gatccgctcc
     3301 gcctcttccc ggccgcccca gccgtccagc tgcacgacct tgtgctcgag gtccttgtag
     3361 cgggcgaaga agtgctcgac ctcctccagc aggtgccccg gcaggtcctc cagctcgtgg
     3421 acgtgcgacc agcgagggtc ggtgtcgggc acgcccagga tcttggcgtc ctcacccttc
     3481 tcgtcgcgca tccgcaggta gccgacgacg cgggtgcgga tggtgcagcc cgggaaggtg
     3541 ggctcgccca ggatgaccac ggcgtcgagc gggtcgccgt cctcgcccca gcagtcgtgg
     3601 acaaagccgt agtcgccggg gtagtgcatg gcgctgaaca gcatgcggtc gagcacgaag
     3661 cggccggcgt cgtggtccca ctcgtacttg ttgcgcgagc cagtcgggat ctcgacgaag
     3721 acatcgacga cggtgctgtc cgacttcacg agctgcctcc tcccatgcgc gactccaggc
     3781 cggcggcgat ctcgtcgcgc agggcggcgt cgcccgcctc gtcgacgcgc aggtgggcgg
     3841 cgtcgcgcca cgcgcgctgc acgtcgaact ccatcgtgta gccgtagccg ccgtgcaact
     3901 gcaggcagcg gtcggcgacg gcggcggaca cgcggtccgc gcgcagccgg agggcggccg
     3961 tgtcaccgga ctccagcgca gcgagcgtca ggcgccgggc ggtgtcgtgc accagcgcgc
     4021 gcagcgcgct gagctcggcg gccatgtccg ccagcgcatg gcggttcacc tggaacgtgg
     4081 cgatgggacg cccgaaggcc tcgcgctgca gcgcgtacgc cttggcgtcc tcccacgcca
     4141 gccaggcggc ggcgaccgtc gcggcggcgt ggtccaggcg ccagcggtcc tggcggcgcc
     4201 gcatcagcgc cagcccctcc gcgccgcgcg ccaccacgtc gcctccggcc agccggacgt
     4261 cgcggaactc cacgtcggcg gcgtggccgc cgcggcggcc gaacggcgac gccggcggcg
     4321 tggcatgcca gccgggcagc cgcgtgtcga cgacggccag ggcgatgccc tgctcgtccg
     4381 gcacgaccgc caccgccgtg tcggccacgg cggcgttggc cagcgccggc gccgtgcccg
     4441 acagcagcag cgcgtcgccg tcgggccgca accgcagcgc gccgcccgcg agcgtcacca
     4501 ggagctctcc ggcgtgggca cgctgggcca cggcaggcac gcctgcctcg ctcagcagcg
     4561 cggccgcgtg cgcctgcgcc aggaggtcct ggacgacgcc ggccgagcgg aggcgcccga
     4621 gctcttccca catcgccacg ccggcgagca cgtcgccgcc accggatgcc gccagcgcgg
     4681 tggacagcca cccctgggac gccaccgtcc ggagcacgtc gcgcggccag ccccccgtgc
     4741 gctcccaggc cggggctccc tcggcgccct cccgctccac ccagcggcgc accacggcgc
     4801 gcagcgcctc gtgctcctcg gtgaacagac tcgtgctcat gcgcggcagc gtagccggtg
     4861 ccgccgcggg gaagcccatc ggtggaagga gccctcatga gcacgccact gcccagcccc
     4921 gccgagccgg tgcccctgcc cgatcccggc gagcccacgc cgctgccgga ccccggcagg
     4981 ccggtccccg tgccggatcc cggcgagccg acgccggtgc cggagccgga tgagccgatg
     5041 ccgctgccgg ggccggccga gccgcagccc ctggccgccg ctcaccagcc gagccgatcc
     5101 agacgcgccg cgacgctggc ggcgtagccg ccgccgaaca gcttcacgtg cgcgagcagg
     5161 tggtacagct gcagggccgg ccggcgctcc cgccagccgt ccggcagcgg ccacgcctcc
     5221 tggtaggcgt ggtggaaggc ctccgggaag ccgccgaata cctccgcgaa cgcgaggtcg
     5281 agttcgcggt cggcgaagca caccgcgggg tccaccagcc agcggccgtc caccacgttg
     5341 cccgcccaca ggtcaccgtg gacgaggctc ggcgcgggtg cgtgggcgcc cagcaagtcc
     5401 tgcagcgggc catcgagggc cgcgcgcagg cgccgtcgca gggcgtcgtc cagcccgccg
     5461 gccgacagga aggggcgcac gcgccgctcg gcgtagaact cggcccactg cggccagtcg
     5521 ccgttgcgct ggaccagtga gccgagccgg ttgtcctcgg gccagccgaa ggctttcgcg
     5581 gtggcccggt gcacggtcgc gagcgcgcgg ccgagcccgg cccactccga cgacccctcc
     5641 agccactcca gcaccagcgt gtgcggtgcg acgcccagca ccccgggcac cggagccccc
     5701 gcccgccgga gcgcctccag cccatcggcc tcgatgcggg ccgggtaggg cgtgcgcttc
     5761 acgaacgcgc gccggccatc ggcccacgcg gcctcccagc tctcgcagat gtcgccgccc
     5821 ggcacgcgcc gccaggtctc cggctcgccc aggtccgggg gaagctccgg cctcacgccg
     5881 tcagcaggcc gcgtacgtgc tcgaccacgc cgcgagcggt ccggcgcgcg atgtccacca
     5941 cggcgtggaa gccgtcctcg ccaccgtagt aggggtcggg aacctcgtcg tggtccgcgt
     6001 cagggtcgaa gtcgcggtac aggcgcactt tcgaccgcga cggctcgtcg ggcgccagcg
     6061 cgcgcacgtc gcgcaggttg gagcggtcca tcaccaggat caggtcggag cgccggaagt
     6121 cctcggcggt gatctgcgcg gcggtcccct ccagcgtcag gccggcggcg gccgcggcgc
     6181 gggtcatgcg cgcgtcgggc ggctcgccga ggtgccagtc gccggtgccg gccgaggcca
     6241 cctcgacctg gccgtcgagc ccggcgtcgc gcagcgcctc gcgcagcgcc gcctcggcgg
     6301 tgggcgagcg gcagatgttt cccaggcaca ccacgagaat ccgcatcggc gcggcagcct
     6361 atcctccagc cactcccgcg atgcacagga ggacgacacg catgggcctg aagcccggag
     6421 acaccgcacc cgatttcacg ctgctggacc aggacggcac accgtggacg ctggcggagc
     6481 agcgcggcag gccggtggtg ctgtacttct atcccaagga cgacacgccg ggctgcacca
     6541 cgcagggctg cgacatccgc gatcactggt cgcggttcga ggccgccggg gtcgtggtgg
     6601 ccggcgtctc gccggactcg gcggagagcc acgccaagtt cgcgggcaag tacggcttcc
     6661 cgcacacgct cctggccgac acggcgcgca cggtgatcca gtcctacggc gcgtgggggg
     6721 agcgctccat gtacggcaag aagtacatgg gcgtcgtgcg ctcctcggtg gtcatcgacg
     6781 ccgcgggcaa agtggccgcg gtgttcagtc cgatcaagcc cgcggaccag tcgaagaagg
     6841 cgctggaggc cctcgcggcg ctctagagaa cgccaacgtg agcagaaagg gcggcgggcg
     6901 tcgcggctct gtcaggcccc ggggatgggc aggccgtcgc cgaccttgcg caggacccgg
     6961 ccggggggca cgtccacggt ctccggcggg cggccctccg ggtcgaccgg cagcagggcc
     7021 atggccagcc agccgccgtc ccgcggcgcc gccgaggtga ccttggcctc cttgtcgccc
     7081 acccgcagca cgccggcctc cggttcggcg tccagttcca cgacggccag cgcgcgccgc
     7141 gggcggccga ggttccaggt cttggcgatg gactcctgcc ccgggtagca gcccttgcgc
     7201 aggtgcacgt gggtcggcag gaggccgagt tcctgcggcc gatggccggg ggcgatctcc
     7261 cggccccacg cgggctcgcc gcgggcgatg cgccaggcct cccagtgctc cgcggacgcc
     7321 tcgggcagcg cttcgccggc cagccgggca tccagccagg cggcgcgtcc cagcaggtcc
     7381 acacccccgg agcggtcgcg cacgacgatg ccgccgccgg cttcctggaa cgtcatcggc
     7441 tcggccgggc cggccggcgc accgccgcgg agggacgcca tggcccagtc ggcggactcg
     7501 tcggcgacct cgacgcgcag caggaagcgc atgccgtcca gccgctccgc cagcgcgggc
     7561 gccacgtcgg gcggcgtgac gagcaggacg tcggcgtccc gcaccagcgc gcggcccgcc
     7621 gcgacgggcg cggccttggc gtccaggtac aggaagtcgg ccgcccgccc ggcaggcatg
     7681 tcctccaggt gctgggacag cagcgtgtgc aggaacgaca ggcggtcctc gcccgtcacg
     7741 cgcaccactc cggcggggcg gcggacgaag gcgggggcat ccagcaacag ggccataccg
     7801 tcaaggctag ccgtgtccgg ccacaggtcg ttgtcggggg cggggcgccg tgtcatcctg
     7861 gccgcacaac agagaggagg tggtccgaca catgagttct gacagttcct gtgtgaccgt
     7921 tgaggtgatg gggagctagt tccccccgct tcgccgcaag gcccgggtgc gtgcatccgg
     7981 gaactggcga atccaacctc atcacccgct gatcgctttg tgggagttgg tcacgcccac
     8041 ccacttgtgc gccgggctcg cgcccgcccg ccaaggacgt gacaaaagcg gtgagtgttg
     8101 gatgcacctt cacggcaggc gccccgccat ccggcggggc gcctgctgcg ttccgggcag
     8161 gtttccggcc agttgacccg tgatggccct gtcggggcgg ccggcacggt gcgaggctgc
     8221 gcccatggaa cgcatcgact tgcccgacgc ctccgccaca ccggtggtct accgcgacgc
     8281 gctgctcgag gtgctgggcg gccgtgacgc gctgaccgtc atggcggaga cacttgaggc
     8341 cttgccgcgc ctgctggacg gcctgtccga cgcggagtgg gccgcggcgc ccgagccggg
     8401 cgaatggtcc cccgcagaac tgctcggcca tttcttcgac gtcgatctgg tgttcggctt
     8461 ccgctggcgc ctggtggcca cgtcggaccg gccgacctac cccggctacg acgagaaggc
     8521 gttcatgggg ctggcgcgtc cggaaccggc gcggctgctg acggcatggc gcgggctgcg
     8581 ggaggccaac ctcgcgatgc tgcggacgct gcccgccgag gcgtacgcgc ggctgggcgt
     8641 ccacggcgag cagggtgagg agacgttgcg tgtcgcggtc gacaagttcg ccggccacga
     8701 c
//
LOCUS       DATMKC010000194         7165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_56952, whole genome shotgun sequence.
ACCESSION   DATMKC010000194 DATMKC010000000
VERSION     DATMKC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56952"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VNU01_05655"
     CDS             <1..1657
                     /locus_tag="VNU01_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32140.1"
                     /translation="PHLGYAWQEEGWKKVAGECAARYPTPEEIPGLLAAGVITPGAGE
                     TAEEYIGAKADPVPTLDDDDELVFMAADAGERAPDGEDGPAPSEEGTRYEVAVLDPTS
                     GRTSYVYLFQQEGGSAYDASNGYVTFARDADADEYLDRYSFAEADPEKLGTSNTGYGP
                     NLPGTVCRTDAEHAGRFGGVADGVARPSTDRFPRDGLTVTTDAYRWRATGRWMIRELQ
                     VAKPGAPGTYGADLVDRWKGRAFQQSPDSSISVVGFEDEQVNWEANSTVLGWKAGPVR
                     AIREVWGADSGTNVTKMETFYRDGVAYRYRVRVHPIPPDGLYTSWDYNAGVAQTYFNA
                     LRPEGVPIDGVNDDVGQVDDIGGRPAFFDAPDPTFDLPSAVERWEQVAGAGDNGSLVY
                     IFELVGPSSAVNFLAVPYYRDDMCLDDGTGDDPVARPWPGEASTDPRVVAGYGGPNTP
                     CEQRQGAWGAHGLHFFATGDSDNAFTPLPVTEIDGQQWQWAVPMGAPRNVGESYAQVV
                     RAKSKAVALPQANEPARSGPRPCPPEQARGRGPARCDDGRSAG"
     gene            complement(1683..2918)
                     /locus_tag="VNU01_05660"
     CDS             complement(1683..2918)
                     /locus_tag="VNU01_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWH32141.1"
                     /translation="MYVQATERLRPGDVLAKTVYDDNARPLLRAGVTLDAKLIESLAA
                     RGCASVYLRDGLADDVVPDTLVEEQIRAEIARNLSDTFDGITADAMSVTETAPDVAAA
                     LDRLGPNPIPLTDRTQQQLKAMHAQAASLVEQIVDAPTAGRLASLKAHSTYTFQHSVD
                     VAVIGVLLGVQAGLSIVELRDLAVGCLLHDIGKRFIDEAILDYAGPLSREQRAIIEQH
                     PFMGFELVRRLPLGSILPPHVAYQHHERQNGTGYPRGLRGSNRIMRQSAERLQPKRML
                     LIAEIAAVADVYSAIASDRPYRSAMPPEKVADILLTMAGPHLNEEIVALFMRSVPLYP
                     VGLWVTITTGAHRGRMGVVVGQNSHELHRPRVRLLIDAGGEALAEPVEFDLAADPGVM
                     VESLNPGRSPLLSRSSEAV"
     gene            3103..3582
                     /locus_tag="VNU01_05665"
     CDS             3103..3582
                     /locus_tag="VNU01_05665"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWH32142.1"
                     /translation="MARTETVIAAPVEQVWAALMDPWLYTQWVVGNKRVRGVDGDWPR
                     PGSRFHHTVGLGPLATRDFTRLVTAQEHRRVELDAHAWPVGTARVVLELEPVDGGTRV
                     VMHEEPTRGPAKWMETRLLHGFTHGRNVESLRRLRRMVEHRARSDGDAGAPEGRSAS"
     gene            3653..4204
                     /locus_tag="VNU01_05670"
     CDS             3653..4204
                     /locus_tag="VNU01_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWH32143.1"
                     /translation="MGLWIALGVVVLVLLWAAGAYNRLVGLRNKVREAWSGIDVQLTR
                     RADLIPNLVETVRGYRIHEREVLENVTQARAGLMGASGPRQAAQAENMLEQALGRLFA
                     VAEDYPDLKANENFLALQSELSELEEEIAFSRRFYNALVQELNTAIQRVPTVIVARAF
                     GFSEAEFFQAEDAARAVPGVDFS"
     gene            4204..6039
                     /locus_tag="VNU01_05675"
     CDS             4204..6039
                     /locus_tag="VNU01_05675"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HWH32144.1"
                     /translation="MRAPARLAALLTALVLLAAPAAAQDPSDTGWVIDRFLVQLTVGD
                     DGVVLVEEEIDVDFGNLRKHGIYRVLPVRYELPDDPSRYRVIDVEGIEVTGTAPADIE
                     VQRPSRVGGERDLVLRIGDPDRTVSGRQSYTIRYQVHGALNAFDSHEELYWNVTGNGW
                     PVPIREARAHVRGGQLIRLACYRGPAGSANLCDDSGILPGAFPARAVATQLGPGEGLT
                     VVSGFAKGTVAPGTPRYEERWTLARAFTGSPAAWPLTVATALAAALAVGALLWRSGRD
                     RHALGGVTAAGVVAPGERPRPLGRVTVVPEFRPPDDLRPAQLGVLVDERVDSADVSAT
                     IVDLAVRGHLVIEERETPRLLWFSRTDWTLRRTGEQPDELLPYERRLLDALFASGDEV
                     TLEDLKGTFAQEYRELTADIERDAVDRGWFRGRPTHTRVAWLVGGLALTGLAVACTIA
                     LALFTTVALAGVPLVLGGLAVVVAHRWMPARTSAGSRAFSRTLGFREYIRAAEVDRME
                     FAEAQRLFVAYLPYAVAFGAVDHWARTFAGLGVTTASLGGWYVGHTLPTGPGGGLDRM
                     SAGLSEFSRAVGGSLPVTPASSGSSGFGGGGFSGGGFGGGGGGSW"
     gene            complement(6052..6519)
                     /locus_tag="VNU01_05680"
     CDS             complement(6052..6519)
                     /locus_tag="VNU01_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWH32145.1"
                     /translation="MATTVKFGELPSLKGQVLGTSDWVEVTQEMIDAFGRTTGDEQWI
                     HMDVERAKAESPYGTTIAHGYLTLSLVPLLHKQALELDGVAMGVNYGANKVRFPAPVP
                     SGSRVQGSFKLLEVDEIPGGGYQLVTEVTITVDGADKPSCVAETVSRVYPAPA"
     gene            complement(6562..>7165)
                     /locus_tag="VNU01_05685"
     CDS             complement(6562..>7165)
                     /locus_tag="VNU01_05685"
                     /inference="COORDINATES: protein motif:HMM:NF039596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH32146.1"
                     /translation="PAARRGELLDAADRVIREFGPDASMSAIAAEAGITKPILYRHFG
                     DKAGLYRALAERYASPVFTELSTVLSTLTDRQERMRTTIDTYLRFIESDPQLYRFLMH
                     RALREQPGVASSIAAFTSVVAAGIAEILTREMELSQEQSRSARAWSHGIVGLVQLAGD
                     WWLDSRELTREELVDHLAALLWNGLSGLTADNADVRSLVE"
BASE COUNT          926 a         2610 c         2656 g          973 t
ORIGIN      
        1 cccgcacctg ggctacgcct ggcaggagga aggctggaag aaggtcgccg gcgagtgcgc
       61 cgcgcgctac cccacgccgg aggagatccc gggcctgctg gccgcggggg tcatcacgcc
      121 gggtgccggc gagacggccg aggagtacat cggcgcgaag gccgacccgg tccccaccct
      181 ggacgacgac gacgagctgg tcttcatggc ggccgacgcc ggcgagcgcg cccccgacgg
      241 cgaggacggc cccgcgccga gcgaggaggg cacgcgctac gaggtcgcgg ttctggaccc
      301 caccagcggc cgcaccagct acgtgtacct gttccagcag gagggcggct ccgcctacga
      361 cgcctccaac ggctacgtca cgttcgcccg tgacgccgac gccgacgagt acctggaccg
      421 ctacagcttc gccgaggccg acccggagaa gctgggcacg tccaacaccg gctacggccc
      481 caacctgccc gggacggtgt gccgcacgga cgccgagcac gccgggcgct tcggcggcgt
      541 ggccgacggc gtcgcccgcc ccagcacgga ccgcttcccg cgtgacggcc tgaccgtgac
      601 gacggacgcc taccgctggc gcgccaccgg ccgctggatg atccgcgagc tccaggtggc
      661 caagccgggc gcgcccggca cctacggcgc cgacctcgtg gaccgctgga agggccgggc
      721 gttccagcag tcgcccgaca gcagcatctc cgtggtgggc ttcgaggacg agcaggtcaa
      781 ctgggaggcg aactccaccg tgctcggctg gaaggccggt cccgtgcgcg ccatccgcga
      841 ggtctggggc gccgactccg gcaccaacgt caccaagatg gagacgttct accgggacgg
      901 cgtcgcgtat cgctaccgcg tgcgcgtgca cccgatcccg ccggacgggc tgtacacgtc
      961 ctgggactac aacgccgggg tggcgcagac ctacttcaac gcgctgcgtc cggaaggcgt
     1021 gccgatcgac ggcgtgaacg acgacgtcgg ccaggtggac gacatcggcg ggcgtccggc
     1081 gttcttcgac gcgccggacc ccaccttcga cctgcccagc gccgtcgagc gctgggagca
     1141 ggtggccggc gccggcgaca acggctcgct ggtgtacatc ttcgagctgg tcgggccgtc
     1201 cagcgccgtg aacttcctgg ccgtgccgta ctaccgcgac gacatgtgcc tggacgatgg
     1261 caccggcgac gacccggtgg cgcgcccgtg gcccggcgag gcgtcgaccg acccgcgggt
     1321 ggtggccggc tacggcggtc cgaacacgcc gtgcgagcag cgccagggcg cctggggcgc
     1381 gcacggcctg cacttcttcg ccaccggcga cagcgacaac gcgttcacgc cgctgcccgt
     1441 gacggagatc gacggccagc agtggcagtg ggccgtgccg atgggcgcgc cgcgcaacgt
     1501 cggggagtcc tacgcgcagg tcgtgcgggc gaagtccaag gcggtcgccc tgccgcaggc
     1561 caacgagccg gcccggtccg gtccgcggcc gtgcccgccg gagcaggccc gcgggcgcgg
     1621 gcccgcccgc tgcgacgacg gccgctcggc gggctgatcc cgcccgcgtc agccggccgg
     1681 ggtcagacgg cctcggacga gcgcgacagc agcggcgacc gccccgggtt gaggctttcc
     1741 accatgaccc cggggtcggc ggccaggtcg aactccacgg gctccgccag cgcctcgccg
     1801 cccgcgtcga tcagcaggcg cacccgtggc cggtgcagct cgtggctgtt ctggcccacc
     1861 accacgccca tgcggccccg gtgcgcgccg gtggtgatgg tcacccacag cccgaccgga
     1921 tacagcggca cggagcgcat gaacagggcg acgatctcct cattcaggtg cggcccggcc
     1981 atcgtcagca ggatgtcggc gaccttctcc ggcggcatgg ccgagcggta cgggcggtcc
     2041 gacgcgatcg cgctgtagac gtcggccacg gccgcgatct cggcgatcag cagcatgcgc
     2101 ttgggctgca gccgctcggc ggactgccgc atgatgcggt tgctgccccg cagcccgcgc
     2161 gggtagccgg tgccgttctg gcgctcgtgg tgctggtagg ccacgtgtgg cggcaggatg
     2221 ctgcccagcg gcaggcggcg caccagctcg aagcccatga acgggtgctg ctcgatgatc
     2281 gcgcgctgct cgcgcgacag cggcccggcg tagtccagga tcgcctcgtc gatgaagcgc
     2341 ttgccgatgt cgtgcagcag gcagcccacc gccaggtcgc gcagctccac gatgctcagc
     2401 ccggcctgca cgcccaggag cacgccgatc accgccacgt ccacggagtg ctggaacgtg
     2461 taggtgctgt gcgccttcag cgacgccaga cgcccggccg tgggagcgtc cacgatctgc
     2521 tcgaccagcg acgcggcctg cgcgtgcatg gccttgagct gctgctgcgt gcggtcggtc
     2581 agcgggatgg ggttgggtcc cagccggtcc agggcggcgg cgacgtccgg cgccgtctcc
     2641 gtcaccgaca tcgcgtcggc ggtgatgccg tcgaacgtgt ccgacaggtt gcgcgcgatc
     2701 tcggcgcgga tctgctcctc caccagcgtg tcggggacca cgtcgtcggc gaggccgtcg
     2761 cgcaggtaca ccgaggcgca gccgcgcgcg gcaagcgact cgatcagctt ggcgtccagc
     2821 gtcacgcccg cgcgcagcag cggccgggcg ttgtcgtcgt acaccgtctt ggccagcacg
     2881 tcgcctggcc gcagccgctc ggtggcctgc acgtacatgc gctgagcgcc tttccaactg
     2941 tgccggggga accgggcccc gtgcatgccg cgtgggcgct gccccggagg gaacgcgata
     3001 ccacgccaga gtgacctctt tgcctgcccg cgtccaccgt caccgcgttc cccgccggcc
     3061 gcggccgctt gacgcggccg ccggcgggga acaacccgcg ccatggcgcg caccgagacg
     3121 gtcatcgccg cccctgtgga gcaggtctgg gcggcgctca tggacccctg gctgtacacg
     3181 cagtgggtcg tgggcaacaa gcgggtccgc ggcgtggacg gcgactggcc ccgccccggc
     3241 agccgcttcc accacaccgt gggcctcggc ccccttgcga cgcgtgactt cacgcggctg
     3301 gtgacggcgc aggagcaccg ccgcgtggag ctggacgcgc acgcctggcc ggtgggcacc
     3361 gcccgcgtgg tgctggagct ggagccggtc gacggcggca cgcgcgtcgt catgcacgaa
     3421 gagcccaccc gcggccccgc caagtggatg gagacgcgcc tgctgcacgg cttcacgcac
     3481 ggccgcaacg tcgagtcgct gcggcgcctg cggcgcatgg tcgagcaccg cgcgcggtcc
     3541 gacggcgacg ccggcgcgcc ggaggggcgc agcgcgtcct gaccctcccc gcgccgtacc
     3601 ctggaggcga cgccggcgcc cggccggcgc gtcgctggga gatggagcgg tcatgggcct
     3661 ttggatcgca ctcggtgtcg tcgtgctcgt cctgctgtgg gcggccggcg cgtacaaccg
     3721 cctcgtgggg ctgcgcaaca aggtccgcga ggcgtggtcc ggcatcgacg tgcagctcac
     3781 gcggcgcgcc gacctgatcc cgaacctcgt cgagaccgtg cgcgggtacc gcatccacga
     3841 gcgcgaggtg ctggagaacg tcacccaggc gcgcgccggg ctgatgggcg cgagcgggcc
     3901 gcgccaggcc gcccaggcgg agaacatgct ggagcaggcg ctgggccggc tgttcgccgt
     3961 ggcggaggac tacccggacc tcaaggccaa cgagaacttc ctggcgctgc agtcggagct
     4021 gtcggagctg gaggaggaga tcgccttctc acggcgcttc tacaacgccc tggtgcagga
     4081 gctgaacacc gcgatccagc gggtgcccac ggtgatcgtg gcgcgcgcgt tcggcttctc
     4141 cgaggcggag ttcttccagg ccgaggacgc ggcgcgcgcc gtgcccgggg tcgacttcag
     4201 ctgatgcgcg cgccggcgcg cctggcggcg ctgctcaccg cgctggtgct gctggcggcg
     4261 cccgccgccg cccaggaccc ctccgacacc ggctgggtga tcgaccgctt cctggtgcag
     4321 ctcacggtcg gcgacgacgg cgtggtgctg gtcgaggaag agatcgacgt cgacttcggc
     4381 aacttgcgca agcacggcat ctatcgcgtc ctgccggtgc gctacgagct gcctgacgac
     4441 ccgtcgcgct accgcgtcat cgacgtcgag ggcatcgagg tcaccggcac ggcgcccgcg
     4501 gacatcgagg tgcagcggcc gtcgcgcgtg ggcggcgagc gggacctggt gctgcgcatc
     4561 ggcgacccgg accgcaccgt gtccggtcgc cagtcctaca cgatccgcta ccaggtgcac
     4621 ggcgcgctga acgccttcga ctcgcacgag gagctgtact ggaacgtcac gggcaacggc
     4681 tggccggtgc ccatccggga ggcacgcgcc cacgtgcgcg gcggccagct catccgcctg
     4741 gcgtgctatc gcggcccggc cggctcggcc aatctctgcg acgacagcgg gatcctgccg
     4801 ggtgccttcc cggcacgggc cgtggcgacg caactcggcc cgggcgaggg cctgacggtg
     4861 gtgagcggct tcgccaaggg caccgtcgcc cccggcacgc cgcgctacga ggagcgctgg
     4921 acgctcgcgc gggcgttcac cggctcgccg gccgcgtggc cgctgaccgt ggccaccgcg
     4981 ctggccgcgg cactggccgt cggtgcgctg ctgtggcggt ccggccgcga ccgccacgcg
     5041 ctcggcgggg tgaccgccgc cggcgtggtc gccccgggtg agcggccgcg gccgctggga
     5101 cgcgtcaccg tggtgccgga gttccggccg cccgacgacc tgcgccccgc gcagctcggc
     5161 gtgctggtgg acgagcgcgt ggacagcgcc gacgtcagcg cgaccatcgt ggacctggcg
     5221 gtgcgcgggc acctggtcat cgaggagcgg gagacgccgc ggctgctgtg gttctcgcgc
     5281 acggactgga cgctgcggcg cacgggcgag cagcccgacg aactgctgcc gtacgagcgg
     5341 cggctgctcg acgcgctgtt cgcgtccggc gacgaggtca ccctggagga cctcaagggc
     5401 acgttcgccc aggagtaccg ggagctgacc gcggacatcg agcgggacgc cgtcgaccgc
     5461 ggctggttcc gcggcaggcc aacccacacg cgggtggcgt ggctcgtcgg cggcctcgcg
     5521 ctcaccggcc tggcggtcgc gtgcaccatc gcgctggccc tgttcaccac ggtcgcgctc
     5581 gccggcgtgc cgctggtgct cggggggctc gccgtggtgg tggcgcaccg ctggatgccg
     5641 gcgcgcacca gtgcgggcag ccgggcgttc tcccggacgc tggggttccg cgagtacatc
     5701 cgcgcggccg aggtcgaccg catggagttc gccgaggcgc agcggctgtt cgtcgcctac
     5761 ctgccctacg cggtggcgtt cggcgccgtc gaccactggg cgcgcacgtt cgcgggcctg
     5821 ggcgtgacca ccgcgtccct gggcggctgg tacgtgggcc acacgctgcc gaccggaccc
     5881 ggcggcggcc tggaccgcat gtcggccggc ctgtcggagt tctcccgcgc ggtcggcggc
     5941 tcgctgccgg tcaccccggc cagttccggc tcgtccgggt tcggcggcgg cggcttcagc
     6001 ggcggcggct tcggcggcgg gggcggcggc tcctggtgaa ccgccgcggc gtcacgcggg
     6061 cgccgggtag acgcggctga cggtctcggc cacgcacgac ggcttgtccg cgccatccac
     6121 ggtgatcgtc acctcggtga cgagctggta gccgccgccg gggatctcgt cgacctcgag
     6181 cagcttgaac gagccctgga cgcggctccc gctcgggacc ggtgcgggga aacgcacctt
     6241 gttggcgccg tagttcacgc ccatcgccac gccgtccagc tccagcgcct gcttgtgcag
     6301 caggggcacc agcgacagcg tcaggtagcc gtgcgcgatg gtcgtgccgt aggggctttc
     6361 cgccttggcg cgctcgacgt ccatgtggat ccactgctcg tcgcccgtgg tgcgcccgaa
     6421 cgcgtcgatc atctcctggg tgacctccac ccagtcgctg gtgccgagca cctgcccctt
     6481 gagggacggc agctcgccga acttcaccgt ggtcgccatg acccgcctcc tgctgtcttc
     6541 gccgcccgtc gggcggacgc cctattcgac cagactgcgc acgtcggcgt tgtcggccgt
     6601 caggccggac aggccgttcc acagcagtgc cgccaggtgg tccaccagct cctcgcgggt
     6661 cagctcgcga gagtccagcc accagtcccc ggccagctgc accagtccca cgatgccgtg
     6721 cgaccaggcg cgcgcggagc ggctctgctc ctggctgagc tccatctccc gcgtgaggat
     6781 ctcggcgatg ccggcggcca ccacgctggt gaacgccgcg atgctggagg ccacgcccgg
     6841 ctgctcgcgc agcgctcggt gcatcaggaa gcggtacagc tgcgggtcgg actcgatgaa
     6901 gcgcaggtag gtgtcgatgg tggtgcgcat gcgctcctgg cggtccgtca gcgtggagag
     6961 cacggtggac agctccgtga acaccgggct ggcgtagcgc tccgccagcg cgcggtacag
     7021 gccggccttg tcgccgaagt ggcggtacag gatgggcttg gtgatcccgg cctccgcggc
     7081 gatggccgac atggaggcgt ccggtccgaa ctcgcggatc acccggtcgg cggcgtcgag
     7141 cagctcgccg cggcgggcgg cgggg
//
LOCUS       DATMKC010000195         7844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_161895, whole genome shotgun sequence.
ACCESSION   DATMKC010000195 DATMKC010000000
VERSION     DATMKC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161895"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VNU01_05690"
     CDS             <1..358
                     /locus_tag="VNU01_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32147.1"
                     /translation="SLMGPEFTISFGADSGPEAGVAGLRQMQERGEDPFALILKLLDM
                     PFTSQEREGGPSPIFTWPSVFGKPAAEWTEQDEAQLRELGDERDIQAWREFGEYGGWR
                     LGIDAAGTWLYLVAGD"
     gene            457..1245
                     /locus_tag="VNU01_05695"
     CDS             457..1245
                     /locus_tag="VNU01_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWH32148.1"
                     /translation="MGTDRVFTDTHCHLDYLEGGEPADAVLERARQAGVSTFITVGVD
                     LATSVVCMQQAMAYPDVWAVVGIHPHNAIEATEHVLERLRDVATHPRVVGIGETGLDW
                     FKDHSPRVRQEESFREHIRLAKELDKALVIHDRDAHEDVVRILDEERAPERTVFHCFS
                     GDEALVGTCAERGWFMSFAGTVTFKNAPVLRKAAAAAPLDLIVAETDSPYLSPHPYRG
                     QVNEPARVTVTVAVLAELHGLSVEEMAARTSANARRLFALPERA"
     gene            1382..2518
                     /locus_tag="VNU01_05700"
     CDS             1382..2518
                     /locus_tag="VNU01_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWH32149.1"
                     /translation="MSRPALSRPHAFTSLLVALLIPLLGFTWLAGEKQVSVMVNGEVR
                     QVRTYAATVDELLDRLGVERGPEDQIVPAAGTALSDGMVVELVHARPITLLVGDVERR
                     VMVTALDVNEVLRELGTEVGRRDIVRPSRLARVVPGMVVEVRRPVAIQVLADGRSHDV
                     ITDARTVAGVLERLNIALGPEDRVLPGLDAAPVADGRIVVQRITRATETRVEAIDFAT
                     VKRRNPDLPKGQERVVEPGKKGLREVTEEVVRADGKVESRRALSERVVRAPVDRVVEV
                     GTKTVERTSPRPANTSGRTPNTQEGKASWYDHPGNDPMTAAHRTLPFGTMVTVTNLAN
                     GKTVTVRINDRGPFVEGRIIDLNREAFQRIAPTSAGVLRVRITW"
     gene            2532..3392
                     /gene="rsmA"
                     /locus_tag="VNU01_05705"
     CDS             2532..3392
                     /gene="rsmA"
                     /locus_tag="VNU01_05705"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWH32150.1"
                     /translation="MALLTPADVRRLLAAHGVHPRKARGQNFVVDPNTVRKVVRDAGV
                     GDGDVVCEVGPGLGSLTLALREAGARVVAVEIDEALARALAEVVAGDVGVRIVQADAL
                     RVDLGAVTDEAARAWQAADAPRLLVANLPYNVATPILMEALATRAFARILVMVQKEVG
                     RRWAAQPRDELYGAVSVKVAAQADVRVAAQVSRSAFHPVPNVDSVTVRLEPRPWAWPV
                     EREALFRLVEAGFAQRRKRLRNSLSRADLAPAAVEDALERAGLDAGARAEELAVAQWV
                     ALAGELQRGG"
     gene            3463..4497
                     /locus_tag="VNU01_05710"
     CDS             3463..4497
                     /locus_tag="VNU01_05710"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF002870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWH32151.1"
                     /translation="MGTGEGGGQRAGSESTPVTREIAADGPCVTVRVPAKVNLFLAVR
                     GVRDDGYHELVTVMQTVSLYDRLRVAVVGPPGQGHHPASRRRMGVELWKDAIDGLPGG
                     ERNLAVQAALALGEAAGVLDVSAVRDAAARSGARTVIDLDKGIPIAGGMAGGSADAAA
                     ALTALNRLWGCELSKDDLREVGAGLGSDVPFCVVGGTALGTGRGSAIAQVLCRGTFHW
                     VVCQAEEPLSTADVYGSWDEHCKPSEIEPDAVLHALRTEDAVALGAALHNDLEAAAFA
                     LRPELEDHKKALLDAGAVGAVLSGSGPTLLALTEDEPSARALAERVADQFRAVTVARS
                     PAGGPEVVSC"
     gene            4541..4614
                     /locus_tag="VNU01_05715"
     tRNA            4541..4614
                     /locus_tag="VNU01_05715"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4573..4575,aa:Gln,seq:ttg)
     gene            4623..6014
                     /gene="glmU"
                     /locus_tag="VNU01_05720"
     CDS             4623..6014
                     /gene="glmU"
                     /locus_tag="VNU01_05720"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWH32152.1"
                     /translation="MSVAAVVLAAGKGTRFRSDLAKVLHPAAGRTMLRWVLEALRPLG
                     LDRVVVVVGHQGEAVAAEARAAGLPGLATVEQAEQLGTGHAVRAAFDAGALDGADRVL
                     VLPGDTPLLRAGDLAPLLDERATVAMLTARLDDPTGYGRVVRDAGGMVQRVVEHRDAT
                     DAERAIDEINAGIYAFPAAALRKELEALSSANAQGEQYLTDVVAPMAMLGIVAVEAPE
                     QAVGGVNDRAQLAAAAAVLRARVNEAHLLAGVTLTDPERTYIGADVRIAPDAVVLPNT
                     HLEGTTRIGAGAVVGPDSRLVDAVVEDGATVAYSVLLEASVGPGATVGPFSYLRPGAR
                     LERGAKAGAFVEVKKSTIGEGSKVPHLSYIGDAEIGRDVNVGAGTITCNFDGRSKHRT
                     VIGDGAFIGSDTMLVAPVAVGPGAVTAAGSAITRDVPADALALERNQQVHVDGYAARR
                     RAQEDNDDESGAS"
     gene            6014..6982
                     /locus_tag="VNU01_05725"
     CDS             6014..6982
                     /locus_tag="VNU01_05725"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWH32153.1"
                     /translation="MEITTKKRMQIFTGRGHPQLAQEVVDHLGMKLGDVDLHEFANGE
                     IYCRYQENVRGSDVFVIQTHTRPVNETIMEQLIMIDAAKRASAKRIIAVCPFYGYARQ
                     DRKARSREPITARLLADMLTQAGADRVVSVNLHTGQIQGFFNQPLDHLTALPLLVGWL
                     INNFNGQELVVASPDAGGVKLASQFQSRLPNATLAVLAKTRTGHNVSQSLGVVGEVAG
                     KTCVLVDDMIDTAGTICGAAEVLMERGAERVIVLATHPVLSDPACERLEKAPIERVVV
                     TNTLPLPAGCDLDKIEVVSIAPIVASTMKAIFEDESVSELFDDQNQ"
     gene            7152..7820
                     /locus_tag="VNU01_05730"
     CDS             7152..7820
                     /locus_tag="VNU01_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWH32154.1"
                     /translation="MSEQVTLAARKRAGNGKGEARSLRREGRVPAITYGTGIDPVPVS
                     VDALELYHALHTDAGANVVLRLEVDGDMHLAFPREIHRHPVRRDVLHVDFVAIDRTKP
                     VTADVPIVVEGAEDAPGASEGGVLEQQLHALSIEVLPLEVPGHLTADVSGMNVNDVLR
                     VSDLAVPSGVTVLTDGEEVVVTCTVPALDVPETPSDEGEAAADVQAEAAEERAEDAAE
                     AAGE"
BASE COUNT         1066 a         2836 c         2933 g         1009 t
ORIGIN      
        1 gtcgctcatg ggcccggagt tcacgatctc cttcggcgcc gacagcggtc cggaggcggg
       61 cgtcgcgggc ctgcgccaga tgcaggagcg cggcgaggac cccttcgccc tgatcctcaa
      121 gctgctggac atgcccttca ccagccagga gcgcgagggc ggcccgtcgc cgatcttcac
      181 gtggccgtcg gtgttcggca agcccgccgc ggagtggacc gagcaggacg aggcgcaact
      241 gcgcgagctt ggcgacgagc gcgacatcca ggcctggcgc gagttcggcg agtacggcgg
      301 ctggcgcctc ggcatcgacg ccgccggcac gtggctgtac ctcgtggccg gcgactgagg
      361 ggcggcacgg cacgcctaga agcggcgcgc cgtcgctggt agcctccgaa gcgcccgcgg
      421 gagcccccgc gggcgcttcg ctgtcaaggc aagatcgtgg ggaccgatcg cgtgttcacc
      481 gacacccact gccacctgga ctacctggaa ggtggcgagc ccgccgacgc cgtgctggag
      541 cgcgcgcgtc aggccggcgt gtccacgttc atcaccgtgg gggtggatct ggccacgtcc
      601 gtggtgtgca tgcagcaggc catggcctac cccgacgtgt gggccgtcgt gggcatccac
      661 ccccacaacg cgatcgaggc caccgagcac gtgctggagc gcctccgcga cgtcgccacg
      721 cacccgcgcg tggtgggcat cggcgagacc gggctggact ggttcaagga ccactcgccg
      781 cgggtgcgcc aggaggagag cttccgcgag cacatccggc tggccaagga gctggacaag
      841 gccctggtga tccacgaccg cgacgcccac gaggacgtgg tgcgcatcct cgacgaggag
      901 cgcgccccgg agcggacggt gttccactgc ttcagcggcg acgaggcgct cgtggggacc
      961 tgcgccgagc gcggctggtt catgtccttc gccgggacgg tgacgttcaa gaacgcgccg
     1021 gtgctgcgca aggccgccgc cgccgcgccg ctggacctca tcgtggccga gaccgactcg
     1081 ccgtacctgt cgccgcaccc ctaccgcggc caggtcaacg agccggcccg cgtcacggtc
     1141 accgtggccg tgctggccga gctccacggc ctgtccgtgg aggagatggc ggcccgcacc
     1201 agcgccaacg cgcgccgcct gtttgccctg ccggagcgcg cctgagagcg ctgcccggat
     1261 aatcccggtt tcccggtcgt cccaggaccc tgctagcctt cgcccgatgc tttggcagag
     1321 gcaggggccc gggagcgccc gtcaagctcc ccccgtccgg catgcgcggc gccgccgcgc
     1381 cgtgtcgcgg cccgctctgt cgcgccccca cgccttcacc tcgctgctgg tggccctgct
     1441 gatcccgctg ctgggcttca cctggctggc gggcgagaag caggtgtcag tcatggtcaa
     1501 cggcgaggtg cgccaggtgc gcacctacgc ggccacggtg gacgagctgc tcgaccgcct
     1561 gggcgtggag cgcgggcccg aggaccagat cgtgccggcc gccgggaccg cgctgtcgga
     1621 cggcatggtc gtggagctgg tgcacgcgcg gcccatcacg ctgctggtcg gcgacgtcga
     1681 gcgccgcgtc atggtgaccg cgctggacgt caacgaggtg ctgcgcgagc tgggcaccga
     1741 ggtcggccgc cgcgacatcg tgcggccgtc gcgcctggcc cgggtggtgc ccggcatggt
     1801 cgtggaggtg cgccgccccg tcgccatcca ggtgctcgcg gacggcagga gccacgacgt
     1861 gatcaccgac gcgcgcaccg tcgccggcgt cctggagcgc ctcaacatcg cgctcggccc
     1921 ggaggaccgc gtgctgcccg gcctggacgc cgcgccggtc gccgacgggc ggatcgtggt
     1981 ccagcgcatc acgcgcgcca cggagacccg tgtggaggcc atcgacttcg ccaccgtcaa
     2041 gcgccgcaac ccggacctgc ccaagggtca ggagcgggtg gtcgagcccg gcaagaaggg
     2101 cctgcgcgag gtcaccgagg aggtcgtgcg cgcagacggc aaggtggagt cgcgccgcgc
     2161 cctgtccgag cgcgtggtgc gggcccccgt ggaccgcgtg gtcgaggtcg gtaccaagac
     2221 cgtggagcgc acgtccccgc ggccggccaa cacctcgggg cgcacgccca acacccagga
     2281 gggcaaggcg tcctggtacg accacccggg caacgacccc atgacggccg cccaccgcac
     2341 cctgccgttc ggcaccatgg tgacggtcac caacctcgcc aacggcaaga ccgtgacggt
     2401 gcgcatcaac gaccgcgggc cgttcgtgga ggggcgcatc atcgacctca accgcgaggc
     2461 cttccagcgc atcgcgccca cctccgccgg cgtgctgcgc gtccgcatca cctggtagcc
     2521 ggcgccggcg catggcactg ctgacccccg ccgacgtccg tcggctgctc gccgcgcacg
     2581 gcgtccaccc gcgcaaggcc cgcggccaga acttcgtcgt cgaccccaac accgtgcgca
     2641 aggtcgtgcg cgacgccggc gtcggtgacg gcgacgtggt ctgcgaggtc ggccccggcc
     2701 tgggcagcct gacgctggcc ctgcgcgagg cgggcgcgcg ggtggtcgcc gtggagatcg
     2761 acgaggcgct ggcgcgggcc ctggcggagg tcgtggccgg cgacgtcggc gtgcgcatcg
     2821 tccaggcgga cgcgctgcgc gtggacctgg gcgccgtcac cgacgaggcg gcgcgcgcct
     2881 ggcaggcggc ggacgcgccc cggctgctgg tggccaatct gccgtacaac gtcgccacgc
     2941 cgatcctcat ggaggcgctg gccacgcgcg cgttcgcgcg catcctggtc atggtgcaga
     3001 aggaggtcgg ccggcgctgg gcggcgcagc cgcgcgacga gctgtacggg gccgtcagcg
     3061 tcaaggtcgc cgcccaggcc gacgtgcgcg tggcggccca ggtaagccgc agcgcgttcc
     3121 acccggtgcc caacgtcgac tccgtgaccg tgcggctgga gccgcggccg tgggcctggc
     3181 cggtcgagcg cgaggcgctg ttccgcctgg tcgaggcggg gttcgcgcag cggcgcaagc
     3241 gcctgcgcaa cagcctgtcg cgcgccgacc tggcgccggc cgccgtggag gacgcgctgg
     3301 agcgcgccgg cctggacgcc ggggcgcgcg ccgaggagct ggccgtcgcc cagtgggtgg
     3361 cgctggcggg cgagctgcag cgcggtggct gagcacggcc agaggcgcgc cccggctcgt
     3421 gcttttgtgc ccggcgaagt cccgccccta ccatggcccc cgatggggac cggagagggc
     3481 ggtgggcagc gtgccggcag cgagtcgacg ccggtgacgc gcgagatcgc ggccgacggg
     3541 ccgtgcgtga ccgtgcgggt ccccgccaag gtgaacctct tcctggcggt gcgcggcgtc
     3601 cgcgacgacg gctaccacga gctcgtcacc gtcatgcaga ccgtgtccct gtacgaccgc
     3661 ctgcgcgtcg ccgtggtcgg cccgcccggg cagggccacc atcccgcaag ccgccgccgc
     3721 atgggtgtgg agctgtggaa ggacgccatc gacgggctgc cgggcggcga gcgcaacctc
     3781 gccgtgcagg ccgcgctggc gctcggcgag gcggccgggg tgctggacgt cagcgccgtg
     3841 cgggacgccg ccgcgcgctc cggcgcgcgc accgtcatcg acctggacaa gggcatcccg
     3901 atcgccggcg gcatggcggg cgggtccgcc gacgcggccg cggcgctcac cgcgctcaac
     3961 cgcctgtggg gctgcgagct gtccaaggac gacctgcgcg aggtcggcgc gggcctgggc
     4021 agcgacgtgc cgttctgcgt ggtcggcggc acggcgctgg gcaccggccg cggcagcgcc
     4081 atcgcgcagg tgctgtgccg gggcaccttc cactgggtgg tgtgccaggc ggaggagccg
     4141 ctgtccaccg cggacgtcta cgggtcgtgg gacgagcact gcaagcccag cgagatcgag
     4201 ccggacgcgg tgctgcacgc cctgcgcacg gaggacgccg tggcgctcgg cgccgcgctg
     4261 cacaacgacc tggaggcggc tgccttcgcg ctgcgcccgg agctggagga ccacaagaag
     4321 gcgctgctgg acgccggcgc cgtcggggcg gtgctgtccg gcagcggccc cacgctgctg
     4381 gcgctcacgg aggacgagcc gtccgcgcgg gcgctcgccg agcgcgtcgc cgaccagttc
     4441 cgcgccgtga cggtcgcgcg ctccccggcc gggggccccg aggtcgtctc ctgctaggcc
     4501 acctgccgta gcatggtcat cgggccatgc cgcccaccag tgggaggtgg tgaaatggca
     4561 tcacgtcgtc ctttggagtc gaagttgaag gttcgatccc ttccctccca gccaggatcc
     4621 gcatgagcgt cgccgctgtg gtgctggcgg cgggcaaggg cacgcgcttc cgctccgacc
     4681 tcgccaaggt gctccacccc gccgcgggcc gcaccatgct gcgctgggtg ctggaggcgc
     4741 tgcggcctct gggcctggac cgcgtggtcg tcgtggtcgg ccaccagggc gaggcggtcg
     4801 ccgccgaggc gcgcgccgcc ggcctgcccg gcttggcgac cgtggagcag gccgagcagc
     4861 tcggcaccgg ccacgccgtg cgcgccgcct tcgacgcggg cgccctcgac ggcgccgacc
     4921 gcgtgctggt gctgccgggc gacacgccgc tgctgcgcgc cggggacctc gcgccactgc
     4981 tcgacgagcg tgccacggtc gcgatgctga cggcgcgcct ggacgacccc accggctacg
     5041 gccgggtggt gcgcgacgcg ggcggcatgg tccagcgcgt cgtggagcac cgcgacgcca
     5101 ccgacgccga acgcgccatc gacgagatca acgcgggcat ctacgcgttc cccgcggccg
     5161 cgctgcgcaa ggagctggag gccttgagca gcgcgaacgc gcaaggcgag cagtacctga
     5221 ccgatgtggt cgcgcccatg gcgatgctcg gcatcgtcgc ggtcgaggcg cctgagcagg
     5281 ccgtcggggg cgtgaacgac cgcgcgcagc tcgccgccgc cgccgccgtc ctgcgggcgc
     5341 gcgtgaacga ggcgcacctg ctcgccggcg tcacgctcac cgacccggag cgcacctaca
     5401 tcggcgcgga cgtgcgcatc gcgcccgacg ccgtggtgct gcccaacacg cacctggagg
     5461 gcaccacgcg catcggcgcc ggcgccgtcg tcggcccgga ctcgcgcctg gtggacgcgg
     5521 tcgtggagga cggcgccacc gtggcgtact cggtcctgct ggaggcgtcg gtcgggccgg
     5581 gcgcgaccgt ggggccgttc agctacctgc gccccggcgc gcgcctggag cgcggcgcca
     5641 aggcgggcgc gttcgtggag gtcaagaagt ccaccatcgg tgagggcagc aaggtgccgc
     5701 acctgtccta catcggcgac gcggagatcg gccgggacgt caatgtcggc gccggcacga
     5761 tcacgtgtaa cttcgacggg cggagcaagc accgcacggt gatcggcgac ggcgcgttca
     5821 tcggcagcga cacgatgctg gtcgcgccgg tggcggtcgg gccaggcgcc gtgaccgcgg
     5881 cgggctccgc gatcacgcgc gacgtccccg cggacgccct cgcgctggag cgcaaccagc
     5941 aggtgcacgt ggacggctac gccgcgcggc ggcgcgccca ggaagacaac gacgacgagt
     6001 cgggagcctc ctgatggaga tcaccaccaa gaagcgcatg cagatcttca ccggccgggg
     6061 ccacccccag ctggcgcagg aagtcgtcga ccacctgggc atgaagctgg gtgacgtcga
     6121 cctgcacgag ttcgccaacg gtgagatcta ctgccgctac caggagaacg tgcgcggctc
     6181 cgacgtgttc gtgatccaga cgcacacgcg cccggtgaac gagacgatca tggagcagct
     6241 catcatgatc gacgcggcca agcgcgcgtc cgccaagcgc atcatcgccg tgtgcccctt
     6301 ctacggctac gcgcgccagg accgcaaggc gcgctcccgc gagccgatca ccgcgcggct
     6361 gctggccgac atgctcacgc aggcgggcgc cgaccgcgtg gtgtcggtga acctgcacac
     6421 cgggcagatc cagggcttct tcaaccagcc gctggaccac ctcacggcgc tgccgctgct
     6481 ggtcggctgg ctgatcaaca acttcaacgg gcaggagctg gtcgtggcct cgcccgacgc
     6541 gggcggggtg aagctggcct cgcagttcca gtcccggctg cccaacgcga ccctggccgt
     6601 gctggccaag acgcgcaccg ggcacaacgt gtcgcagtcg cttggcgtgg tcggcgaggt
     6661 ggccggcaag acctgcgtgc tggtcgacga catgatcgac actgcgggca ccatctgcgg
     6721 cgcggccgag gtgctcatgg agcgcggggc ggagcgcgtg atcgtgctgg ccacgcaccc
     6781 ggtgctgagc gaccccgcgt gcgagcggct ggagaaggcg cccatcgagc gcgtcgtcgt
     6841 gacgaacacg ctgccgctgc cggccggctg cgacctggac aagatcgagg tggtgtccat
     6901 cgccccgatc gtggccagca ccatgaaggc catcttcgag gacgagtcgg tcagcgagct
     6961 gttcgacgac cagaaccagt agccgtcccc gttcgaacgg gtgcgggcac cgcgagatcc
     7021 cgcaatgggg gcacccgttc gcgtccggtt gccggggccg ggcaagcctg gctacactcg
     7081 gggagtcgcg cgtcggccag ctgggccgcg cgcccttttt tgaaccagcc gcattcagga
     7141 gtgaaccagc catgtctgag caggtgacgc tggctgcccg caagcgagcg ggcaacggca
     7201 agggtgaggc gcgcagcctc cgccgtgagg gccgtgtccc cgcgatcacg tacggcacgg
     7261 gcatcgaccc cgtgccggtg tccgtggacg cgctggagct ctaccacgcc ctgcacaccg
     7321 acgcgggggc caacgtggtg ctgcggctgg aggtcgacgg ggacatgcac ctggccttcc
     7381 cccgcgagat ccaccgccac ccggtgcgcc gtgacgtcct gcacgtcgac ttcgtcgcga
     7441 tcgaccgcac caagccggtc accgcggacg tgccgatcgt ggtcgagggc gccgaggacg
     7501 cgcccggcgc cagcgagggc ggcgtgctgg agcagcagct ccacgccctg tccatcgagg
     7561 tgctgcccct cgaggtcccg gggcacctca ccgccgacgt ctccggcatg aacgtgaacg
     7621 acgtgctgcg cgtgtcggat ctggccgtcc cctccggcgt cacggtgctg accgatggcg
     7681 aagaggtcgt cgtgacctgc accgtcccgg cgctggacgt cccggagacg ccgagcgacg
     7741 agggcgaggc tgcggcggac gtccaggccg aggccgccga ggagcgcgcc gaggacgccg
     7801 ccgaggctgc cggcgagtag gccgctgccg tgggcctgct gtcc
//
LOCUS       DATMKC010000196         4184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_72789, whole genome shotgun sequence.
ACCESSION   DATMKC010000196 DATMKC010000000
VERSION     DATMKC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72789"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VNU01_05735"
     CDS             <1..467
                     /locus_tag="VNU01_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWH32155.1"
                     /translation="LVPRDLVVMGLSALVLIGIGVLLEGTRVGKAMRAVADNRDLAES
                     SGIDVQRVILFVWFFGGALAALGGVFVGVSENVNWLMGFRMLLLMFAGTILGGLGTAY
                     GALAGSILVGLVTEVSTVWFSPELKYVWALLILGVVLMLRPQGLLGVKERIG"
     gene            467..1456
                     /locus_tag="VNU01_05740"
     CDS             467..1456
                     /locus_tag="VNU01_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWH32156.1"
                     /translation="MDFVRVIGDALRAALGLEAAVYALAAIGLNVHFGYTGLLNFGQV
                     GFMLVGAYGLAITVSTFGGSMWLGMLVGVLLAVVLAILLGGPTLRLRADYLAITTIAA
                     AEILRFTFRSSPATGVTGGVFGLQRFADAFYEINPLPQGTYGFWVVQFSHRRMWVMLV
                     AWGLVALSCLMVWALMHSPWGRVLKSIREDEEAARSLGKNVFSFKMQGLILGGVFGAL
                     SGIFLAIGQQAVSPDTYIPQITFFTYTVLILGGAARVFGPVLGSVLFWLLIAGTDSFI
                     RRAASAGMLPEVFFGSDTIGAVRFMLVGAGLMILMIYRPEGILGDRREMQLDV"
     gene            1449..2297
                     /locus_tag="VNU01_05745"
     CDS             1449..2297
                     /locus_tag="VNU01_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32157.1"
                     /translation="MSDVSASQPAADPLAGVAPEPGVTKPDPILVVDGVRRSFGGLVA
                     VDVEHLEVQRGCITSLIGPNGAGKTTFFNLMTGFDRPDEGRWMFDGEPLARVPAHKVA
                     RRGMVRTFQLTKALQRMTVIDNMRLAATGQRGERFLGAMLHGSWREQEQDVTRRSEEL
                     LARFKLDAKRDDYAGTLSGGQRKLLEMARALMVEPKMVMLDEPMAGVNPALTQSLLEH
                     VKGLRDLGMTVLFVEHDMDVVMDISDWVVCMAEGRIIAEGPPRAIAQNEAVIEAYLGR
                     ADDEVA"
     gene            2297..3043
                     /locus_tag="VNU01_05750"
     CDS             2297..3043
                     /locus_tag="VNU01_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32158.1"
                     /translation="MSDANGGKPLLEADHLVAGYVPGVNILNGCSLDLREGELVGIIG
                     PNGAGKSTLIKAMFGLVPVAEGRVVLDGHDITNRPAHELVALGVGYVPQVANVFQRLT
                     VQENLEMGLYLEPGKFDERFAAVAELFPLLAQRRKQRAGSLSGGERQMVAMGRALMMR
                     PKVLFLDEPSAGLSPANQTEVFRRIRQINESGISVVMVEQNARRCLQIAHRGYVLDQG
                     RNAHTGSGEELLHDPRVIELYLGTLGRAAE"
     gene            complement(3104..>4184)
                     /locus_tag="VNU01_05755"
     CDS             complement(3104..>4184)
                     /locus_tag="VNU01_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH32159.1"
                     /translation="GPPMIKAMEFAVDQINAAGGVLDQDVTVSGGDEAGDAAVASTSA
                     DQVLSEGVDAVIGAASSGMSLAIIDKITQAEVVQCSGSNTSPTFTNYDDGGYYFRTAP
                     TDALQGPVLAETIAGDGHTKVALMGRGDDYGKGLVNATKEALEEQGAEVVAEVIYDPE
                     AASFDAEVQQVTAANPDAIAVIAFDEGSQIIKGLIEAGVSADQLYGADGVRSNDLAGL
                     VDPNNAAVLEGMKGTAPDPSADEAFLSDLQEFAPDLNDTTFAGQVYDCVVVIALAAIA
                     AGSDSPSAFKDEIVGVTRDGEKCDSFESCKQLLEDGEDIDFDGASGPIEFTDAGEPET
                     GMYEVWTINAEGQVETVSTVESTLE"
BASE COUNT          557 a         1410 c         1514 g          703 t
ORIGIN      
        1 ccctggtgcc gcgcgacctg gtggtcatgg ggctgtccgc gctggtgctg atcggcatcg
       61 gggtgctgct ggagggcacg cgcgtcggca aggccatgcg cgccgtcgcc gacaaccgcg
      121 acctcgccga gtcctccggc atcgacgtgc agcgcgtgat cctgttcgtg tggttcttcg
      181 gcggcgcgct cgcggcgctg ggcggcgtgt tcgtgggcgt gtcggagaac gtcaactggc
      241 tcatgggctt ccgcatgctg ctgctgatgt tcgccgggac gatcctgggc gggctcggca
      301 cggcgtacgg cgcgctggcg ggcagcatcc tcgtcggcct ggtcaccgag gtgagcacgg
      361 tgtggttctc gccggagctc aagtacgtct gggccctgct gatcctgggc gtggtgctga
      421 tgctccgccc gcagggcctg ctgggcgtca aggagcggat cggctgatgg atttcgtgag
      481 ggtcatcggt gacgcgctgc gggccgccct gggcctggag gcggccgtgt acgcgctggc
      541 ggccatcggc ctgaacgtgc acttcggcta caccggcctg ctcaacttcg gccaggtggg
      601 cttcatgctg gtcggcgcct acggcctggc catcaccgtg tcgacgttcg gcggcagcat
      661 gtggctcggc atgctggtcg gggtcctcct ggccgtggtg ctggcgatcc tgctgggcgg
      721 gccgacctta cgcctgcgcg cggactacct ggcgatcacc accatcgccg cggcggagat
      781 cctgcgcttc acgttccggt cgagccccgc cacgggagtc accggcggcg tgttcggcct
      841 ccagcggttc gcggacgcct tctatgagat caacccgctg ccgcagggca cctacggctt
      901 ctgggtggtg cagttctcgc accggcggat gtgggtgatg ctggtggcgt ggggcctcgt
      961 cgcgctgagc tgcctgatgg tgtgggcgct catgcacagc ccgtggggcc gcgtgctgaa
     1021 gtcgatccgc gaggacgagg aggccgcgcg cagcctcggc aagaacgtgt tctcgttcaa
     1081 gatgcagggg ctgatccttg gcggggtgtt cggtgcgctg tcggggatct tcctggccat
     1141 cggccagcag gcggtgagcc cggacaccta catcccgcag atcacgttct tcacctacac
     1201 ggtgctgatc ctgggcgggg cggcgcgcgt gttcggcccc gtgctgggca gcgtgctgtt
     1261 ctggctgctc atcgcgggga ccgactcgtt catccgcagg gccgcgtcgg ccgggatgct
     1321 gcccgaggtg ttcttcggct cggacacgat cggcgcggtg cgcttcatgc tcgtgggcgc
     1381 gggactgatg atcctgatga tctaccgccc cgagggcatc ctgggggacc gacgggagat
     1441 gcagctcgat gtctgacgtg tccgcttccc agcccgccgc cgaccccctg gccggtgtcg
     1501 cgccggagcc gggcgtcacc aagcccgacc cgatcctggt ggtcgacggc gtgcgccgca
     1561 gcttcggcgg cctggtcgcc gtggacgtgg agcacctgga ggtgcagcgc ggctgcatca
     1621 cgtcgctgat cggcccgaac ggcgccggca agaccacatt cttcaacttg atgaccggct
     1681 tcgaccggcc cgacgagggg cggtggatgt tcgacgggga gccgcttgcc cgcgtgccgg
     1741 cgcacaaggt cgcccgccgc ggcatggtgc gcacgttcca gctcaccaag gcgctgcagc
     1801 gcatgaccgt catcgacaac atgcgcctgg ccgcgacggg ccagcgcggc gagcgcttcc
     1861 tgggcgccat gctgcacggc tcgtggcgcg agcaggagca ggacgtgacg cgccgctcgg
     1921 aggaactgct cgcgcgcttc aagctggacg ccaagcgcga cgactacgcg ggcacgctgt
     1981 cgggcggcca gcgcaagctg ctggagatgg cccgggcgct catggtggag cccaagatgg
     2041 tgatgctgga cgagcccatg gcgggcgtga acccggcgct gacgcagtcg ctgctggagc
     2101 acgtcaaggg cctgcgcgac ctgggcatga cggtgctgtt cgtggagcac gacatggacg
     2161 tggtcatgga catctccgac tgggtggtgt gcatggccga gggccgcatc atcgcggagg
     2221 gaccgccacg ggccatcgcg cagaacgagg ccgtgatcga ggcctacctg ggccgcgccg
     2281 acgacgaggt cgcctgatgt ccgacgcgaa cggcggcaag ccgctcctgg aggccgacca
     2341 cctcgtcgcg ggctacgtgc cgggcgtcaa catcctcaac ggctgctcgc tcgacctgcg
     2401 ggagggcgag ctggtaggga tcatcggccc caacggcgcc ggcaagtcca cgctcatcaa
     2461 ggccatgttc ggcctggtgc cggtcgccga gggccgggtc gtcctcgacg gccacgacat
     2521 caccaaccgc ccggcgcacg aactggtcgc gctcggcgtg ggctacgtgc cgcaggtcgc
     2581 caacgtgttc cagcgcctca ccgtccagga gaacctggag atgggcctgt acctggagcc
     2641 cggcaagttc gacgagcgct tcgccgccgt ggcggagctc ttcccgctcc tggcgcagcg
     2701 gcgcaagcag cgcgccgggt cgctgtccgg cggcgagcgc cagatggtgg ccatgggccg
     2761 cgccctgatg atgcggccga aggtgctgtt cctggacgag ccctccgccg gcctgtcgcc
     2821 ggcgaaccag accgaggtgt tccgccgcat ccgccagatc aacgagagcg ggatctccgt
     2881 cgtgatggtg gagcagaacg cccgccgctg cctgcagatc gcccaccgcg ggtacgtgct
     2941 ggaccagggc cgcaacgccc acaccggctc cggcgaggag ctgctgcacg accccagggt
     3001 catcgagctg tacctcggca cgctgggacg cgctgccgag taaacggctg cgtgaatgca
     3061 gaaggggccc gccggagcgg gccccttcgc atcgcgtcga gccttactcc agggtggact
     3121 ccacggtgga caccgtctcg acctgaccct cggcgttgat ggtccagact tcgtacatgc
     3181 cggtctcggg ctcacccgcg tcggtgaact cgatcggacc gctggctccg tcgaagtcga
     3241 tgtcctcgcc gtcctccagg agctgcttgc agctctcgaa ggagtcgcac ttctcgccgt
     3301 cgcgcgtcac gccgacgatc tcgtccttga acgcggacgg gctgtcgctg ccggccgcga
     3361 tcgcggccag ggcgatcacg acgacgcagt cgtacacctg gccggcgaac gtggtgtcgt
     3421 tgaggtccgg ggcgaactcc tgcaggtcgc tgaggaaggc ctcgtcagcg ctggggtccg
     3481 gagccgtgcc cttcatgccc tcgagcaccg cggcgttgtt cgggtccacc agcccggcca
     3541 ggtcgttgga gcggacaccg tccgcgccgt agagctggtc ggcggagacg ccggcctcga
     3601 tcaggccctt gatgatctgc gagccctcgt cgaaggcgat caccgcgatc gcgtcggggt
     3661 tggcggccgt gacctgctgg acctccgcgt cgaagctcgc ggcctccggg tcgtagatga
     3721 cctcggccac gacctcggcg ccctgctcct ccagcgcctc cttggtggcg ttcaccaggc
     3781 ccttgccgta gtcgtcaccg cggcccatga gggccacctt ggtgtgaccg tcgccggcga
     3841 tggtctccgc cagcacgggg ccctgcaggg cgtcggtcgg cgccgtgcgg aagtagtagc
     3901 cgccgtcgtc gtagttcgtg aaggtcgggc tggtgttgga gcccgagcac tgcacgacct
     3961 cggcctgcgt gatcttgtcg atgatcgcga gcgacatgcc ggaggacgcc gcaccgatga
     4021 ccgcgtccac gccctcgctg aggacctggt cggccgaggt gctggcgacc gcggcgtcgc
     4081 cggcctcgtc accgccgctg acggtcacgt cctggtccaa gacgccgccg gccgcgttga
     4141 tctggtcgac cgcgaactcc atggccttga tcatgggggg gccg
//
LOCUS       DATMKC010000197         7092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_8342, whole genome shotgun sequence.
ACCESSION   DATMKC010000197 DATMKC010000000
VERSION     DATMKC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8342"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VNU01_05760"
     CDS             <1..952
                     /locus_tag="VNU01_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH32160.1"
                     /translation="DWTVPRPDGYARWRDEVLPGWPGPRFAFAAPDPKTGATRRHTFT
                     PNPPGDPAALRVDLTAEGGADELWTFRRIAARGLFEPGAYPSDLTLVNWPMVDYTDGP
                     VHGGTAEQDAAHLDGAKRLSLAFLHWLQTEAPRPDGGTGWPGLRPEPEATGTPDGFAK
                     AAYIRESRRIRALHTVTEQQVALDARGDAAAVAFPDSVGIGAYRIDLHPSTGGDGYLD
                     VGAAPFAIPLGALLPVRLRNLLPAAKNLGTTHITNGCYRLHPVEWNVGEAAGRLAAHC
                     LARGLEPRQVRADPALLAEFQDLLRRTGVELEWPSEVRGY"
     gene            complement(956..3631)
                     /locus_tag="VNU01_05765"
     CDS             complement(956..3631)
                     /locus_tag="VNU01_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWH32161.1"
                     /translation="MTIPAVSDETPRPEVADPRAADGVRTQARYRALVEATALDVWHA
                     DAAGALVRDMPNWRAITGQRPGDVLGEGWQLAIHPDDVERVRATWQLAVATRSIYECE
                     YRIVARDGSHRVIHARGVPVVEDGELVEWVGTSEDVTDDREEERYTREQARAVERLSE
                     IGTTLSAELDVRELLQTITDATTELSGAQFGAFFYSEVRQDDGVEQEQFALYTVSGVP
                     LEKFSRFPMPRNTAIFAPTFAGEGPVRLDDVTQDARYGRNPPYHGMPEGHLPVRSYLA
                     VPVVSKSGHVHGGLFFGHELPGVFNARHERLVVSVAAQAAAALDNAQLYEAERRSRAA
                     AEQAQRDLAFLARANYALGASLDFHKSAEVVTKLPVPDLCDWAVLHVVSEGGDVEAVS
                     LYHRDEARRQLLRELAGRFPVRLDEERGPAVALRTGQTYVLEAIDDAALDAHARDDRH
                     REALDQAGIGAMLSVPILTRDRVVGSLSFVRERSRSFTDREVVLAEELTSRAGLALDS
                     AMLFSRERAAALTLQRSLLPGALPAVEGTVCAVRYLPGAAGMEVGGDWYDVIPLGEGV
                     VAYVVGDVMGRGVQAAGVMGQIRSAVRAYALEDHPPEEVLARVDRLLRLAEEPPLVTC
                     VYARLDTRSGELCLSTAGHLPPLVVSPLGESRYLELEPGLPLGVAETTFASGCLTLER
                     GSTLVLYTDGLVETPQSSVTAGMEALRACAVEYAVEGDIEAFIDALISALERGDGHDD
                     DIAVLAVRLPEATEVLPADAPAGRAVGRTLPPDASSVGKARAFLHETLAAWDVPVDID
                     DAKLLVSELVTNALVHAGTVVHLHVEAREDALRVAVGDEGPPDREVPTGGELPDLRMA
                     VGGRGLAIVSVLAHRWGVDPLPDGKRVWFELGFAQ"
     gene            complement(3730..5673)
                     /locus_tag="VNU01_05770"
     CDS             complement(3730..5673)
                     /locus_tag="VNU01_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32162.1"
                     /translation="MSARFVAASVALLAAAVLFAAPPTDDSGRPRAEDVARLRRVAYA
                     ELRVDGPRPPFRPYPAAAIGLATPAGDPPRARGRVQTVAGPREDWRLALADGALPFTY
                     EVDRELARRVGLGTVIEAVSAWDGVTGSRWRTRFAGLSRRPLDEAYPDGRSTVYLRTR
                     CPAGLLGSVRWQLGPSVATLDVRGGTGAVFTSEVDIAVCPLAERGDRVRRTLLHEVGH
                     VMGLEHLCERGRPCPPAVDGVPADCRVMHWLLRGCGERIRDAERDLARHLYPTLPRLW
                     GPGAAETAVRASYATVPTQSARRVVLARPGTHPWLLAPVLLEAAPDDAPLLFAEPELG
                     GCVPAPAGGELARAAGPGAEVLLVGAWPPSCDEVLAGWGLRVRRRMVSPSGHGWTGAD
                     RMRAQIDAAELVLRRHAEDGRPSTELVLAPLPAGVHAGPLDGEETALAWSAAAVAAAR
                     RAPLLLVAGPRLPSAVAAFLREHAEIRTVHAVGGATRLHPLLLHQLRRTGRATTRASG
                     VSAPRVTAATAAAFNAGAPLFAAPVATPDEQALAASLAARTGGMMAVVGGGSARPLRD
                     AAAPRRPAGGALVAGPGDVPATVHWSLTEVVLPASDPRLDVERVRAEPPGSRLREALG
                     RAAPHWTAARDGAGFAVGPRRLG"
     gene            5783..>7092
                     /gene="nhaA"
                     /locus_tag="VNU01_05775"
     CDS             5783..>7092
                     /gene="nhaA"
                     /locus_tag="VNU01_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HWH32163.1"
                     /translation="MSHGHGPTLSDEPGLGIPFSRRDALVPRLVVQPLQRFAQTETSA
                     GVLLALMAIVALIWANSGWYENYHHLWETEIGLDVGGWGLHLTLHEWINDGAMAIFFF
                     VVGMEIKRELVHGQLRDPRAAALPVFAAVGGMVVPALIYTALNGGEPSSNGWGIPMAT
                     DIAFAVGALSLVGKRLPTSLKIFLLTLAVADDLGAIAVIAIFYSTGVQLQWIGIVALT
                     AAAVWVLQRMHVRWLAPYVVLALFLWFATHESGVHATIAGVVLGFLTPAHPFHPPKRA
                     AQVLHRALDRVTADKRGEDSDVDEYELLDVARLSREAVSPLARLENALHPWSAYLILP
                     LFALSNAGVRVVDSDFGELLTRPVTLGVMLGLVVGKPLGVVAASWIATKLRIGVLPAG
                     VTWPHVTAVGLFAGIGFTVALFVSGLSFTDPDLSDGSKVGILLAS"
BASE COUNT          890 a         2802 c         2556 g          844 t
ORIGIN      
        1 ggactggacc gtgccgcgcc cggacggcta cgcgcgctgg cgcgacgagg tgctgcccgg
       61 ctggccggga ccgcgcttcg ccttcgcggc gccggacccc aagaccgggg cgacgcgccg
      121 gcacacgttc acgccgaacc cgccgggcga tccggcggcg ctcagagtcg acctcaccgc
      181 cgagggcggc gccgacgagc tgtggacgtt ccgccgcatc gccgcgcgcg gcctgttcga
      241 gcccggcgcc taccccagcg acctcacgct ggtgaactgg ccgatggtcg actacaccga
      301 cgggccggtg cacggcggca ccgccgagca ggacgcggcg cacctggacg gcgccaagcg
      361 gctgagcctg gcgttcctgc actggctgca gacggaggcg ccgcggccgg acggcggcac
      421 cggctggccg ggcctgcgcc cggagcccga ggcgaccggc acccccgacg gcttcgccaa
      481 ggccgcctac atcagggagt cccggcgcat tcgcgcgctg cacaccgtca ccgagcagca
      541 ggtggcgctg gacgcgcgcg gcgacgcggc cgccgtcgcc ttccccgaca gcgtcggcat
      601 cggggcctac cgcatcgacc tgcacccctc caccggcggg gacggctacc tggacgtcgg
      661 cgccgcaccg ttcgccatcc cgctcggcgc gctgctcccg gtgcggctgc gcaacctcct
      721 gcccgccgcc aagaacctgg gcaccaccca catcaccaac ggctgctacc ggctgcaccc
      781 cgtggagtgg aacgtcggcg aggcggcggg ccgcctggcc gcgcactgcc tggcccgggg
      841 cctggagccg cggcaggtgc gcgccgaccc ggcgctgctg gcggagttcc aggacctgct
      901 gcgccggacg ggggtggagc tggagtggcc gagcgaggtg cggggctact gagccctact
      961 gcgcgaagcc cagctcgaac cacacgcgct tgccgtcggg cagagggtcc acgccccagc
     1021 gatgggcgag cacggacacg atcgccaggc cgcgtccgcc caccgccatg cgcaggtccg
     1081 gcagctcgcc gcccgtgggc acctcccggt ccggcgggcc ctcgtcgccc acggcgacgc
     1141 gcagtgcgtc ctcgcgcgcc tcgacgtgca ggtgcaccac ggtgcccgcg tgcaccagcg
     1201 cgttggtcac cagctcgctg accagcagct tcgcgtcgtc gatgtccacc ggcacgtccc
     1261 aggcggccag cgtctcgtgc aggaaggcgc gggccttgcc caccgaggag gcgtccggcg
     1321 gcagcgtgcg gccgaccgcg cggccggccg gggcgtccgc cggcaggacc tcggtcgcct
     1381 ccggcaggcg gaccgccagc accgcgatgt cgtcgtcgtg gccatcgccg cgctccagcg
     1441 cgctgatcag cgcgtcgatg aacgcctcga tgtcgccctc cacggcgtac tcgaccgcgc
     1501 acgcgcgcag cgcctccatg ccggccgtga ccgacgactg cggggtctcc accaggccgt
     1561 cggtgtagag caccagcgtg ctgccccgct ccagcgtcag gcatcccgac gcgaacgtcg
     1621 tctccgccac gcccagcggc aggccgggct ccagctccag gtagcggctc tcccccagcg
     1681 ggctgaccac cagcggcggc aggtggccgg ccgtggacag gcacagctcg ccagagcggg
     1741 tgtccagccg cgcgtacacg cacgtgacca gcggcggctc ctcggccagc cgcagcagcc
     1801 ggtccacgcg cgccagcacc tcctccggcg gatggtcctc gagcgcgtac gcgcgcacgg
     1861 ccgagcggat ctggcccatc acgcccgcgg cctgcacccc gcggcccatg acgtcgccga
     1921 ccacgtaggc gaccacgccc tcgcccaggg ggatcacgtc gtaccagtcg cccccgacct
     1981 ccatgccggc ggcgccgggc aggtagcgca ccgcgcacac ggtgccctcc acggcgggca
     2041 gcgcgccggg cagcaggctg cgctgcagcg tcagggcggc ggcgcgctcg cgggagaaca
     2101 gcatcgcgct gtccagcgcg aggccagcgc ggctggtcag ctcctcggcc aggaccacct
     2161 cccggtccgt gaaggatcgc gagcgctcgc gcacgaacga cagggagccc acgacgcggt
     2221 cacgcgtgag gatcggcacg gacagcatgg cgccgatgcc ggcctggtcc agcgcctcac
     2281 gatgacggtc gtcgcgggcg tgggcgtcga gcgcggcgtc gtcgatcgcc tccagcacgt
     2341 aggtctggcc ggtgcgcagc gccacggcgg ggccgcgctc ctcgtccagg cgcaccggga
     2401 agcgcccggc cagttcgcgc agcagctgcc ggcgcgcctc gtctcggtgg tacaggctca
     2461 ccgcctcgac gtcgccgccc tcgctcacca cgtgcagcac ggcccagtcg cacaggtccg
     2521 gcacgggcag cttggtgacg acctccgcgg acttgtggaa gtccagcgac gcgcccagcg
     2581 cgtagttggc ccgggcgagg aacgcgagat cgcgctgcgc ctgctcggcc gccgcgcgcg
     2641 agcggcgctc cgcctcgtac agctgggcgt tgtccagcgc cgcggcggcc tgcgccgcca
     2701 cgctcaccac cagccgctcg tgccgggcgt tgaacacgcc cggcagctcg tggccgaaga
     2761 acagcccgcc gtgcacgtga ccgctcttgg acaccaccgg caccgccagg tagctgcgca
     2821 ccggcaggtg gccctccggc atgccgtggt acggcgggtt cctgccgtag cgggcgtcct
     2881 gggtgacgtc gtccaggcgc acgggcccct cgccggcgaa cgtcggcgcg aagatggccg
     2941 tgttgcgggg catcgggaag cgcgagaact tctccagcgg cacaccggac acggtgtaga
     3001 gcgcgaactg ctcctgctcc acgccgtcgt cctggcgcac ctcggagtag aagaacgcgc
     3061 cgaactgcgc gcccgacagc tccgtggtcg cgtccgtgat cgtctgcagc agctcccgca
     3121 cgtcgagctc agccgacagc gtggtgccga tctcgctcag gcgctccacc gcccgcgcct
     3181 gctcgcgcgt gtagcgctcc tcctcgcggt cgtcggtgac gtcctcggag gtgccgaccc
     3241 actcgaccag ctcgccgtcc tcgaccaccg gcacgccgcg ggcgtggatg acccggtggc
     3301 tgccgtcgcg ggccacgatg cggtactcgc actcgtagat cgaccgcgtc gccaccgcca
     3361 gctgccaggt cgcgcgcacc cgctcgacgt cgtccgggtg gatcgcgagc tgccagccct
     3421 cgccgagcac gtcgcccggg cgctggccgg tgatggcgcg ccagttcggc atgtcgcgga
     3481 cgagggcgcc ggcggcgtcg gcgtgccaga cgtcgagcgc ggtggcctcg acgagggccc
     3541 ggtagcgcgc ctgggtgcgg acaccgtcgg ccgcccgagg gtccgcgacc tcgggtcgag
     3601 gcgtctcgtc ggagacggcg gggatggtca tcgagctgcg cgtcgtccca tgtcgtggct
     3661 gttcgcccgc acagacgggc caacccgccc atggcaacac gggccgcccg ggcggacaac
     3721 ccctctttcc taccccaatc ggcgcggccc aaccgcaaag cccgcgccgt cgcgagcggc
     3781 ggtccagtgc ggagccgccc ggcccagcgc ctcgcgcagc cgcgaacccg gcggctcggc
     3841 gcgcacccgc tccacgtcca gtcgcgggtc cgacgccggc aggaccacct cggtgagcga
     3901 ccagtgcacc gtcgccggca cgtcgcccgg cccggccacc agggcgccgc cggccgggcg
     3961 ccgcggcgcc gcagcgtccc gcagcggcct ggccgagccg ccccccacga ccgccatcat
     4021 cccgcccgtg cgcgcggcca gggacgccgc cagcgcctgc tcgtcgggtg tggccaccgg
     4081 cgcggcgaac agcggcgcac ccgcgttgaa cgcggccgcg gtcgctgcgg tcacgcgcgg
     4141 cgccgagacg ccggacgcgc gcgtggtggc gcgcccggtg cggcgcagct ggtgcagcag
     4201 gagcgggtgc aggcgcgtcg cgccccccac ggcgtgcacc gtgcggattt cggcatgctc
     4261 ccggaggaag gccgccacgg cgctgggcag ccgcggcccg gccaccagca gcaggggcgc
     4321 gcggcgcgcc gccgcgaccg ccgccgcgct ccacgccagc gccgtctcct ccccgtccag
     4381 cggtccggcg tgcacgccgg cgggcagcgg cgccaggacc agctccgtgg aggggcggcc
     4441 gtcctcggcg tggcggcgca ggacgagctc ggcggcgtcg atctgcgcgc gcatgcggtc
     4501 cgcgcccgtc cagccatggc cggatggact gaccatgcgc cggcgcacgc gcaggcccca
     4561 gcccgcgagc acctcgtcgc aggacggcgg ccacgcgccc accagcagca cctccgcccc
     4621 cggcccggcg gcgcgggcca gctcgccgcc ggccggagcg ggcacgcagc cgcccagctc
     4681 cggctcggcg aacagcaggg gcgcgtcatc gggtgccgcc tccagcagca ccggggcgag
     4741 cagccacggg tgcgtgccgg gccgcgccag caccacgcgg cgggcgctct gcgtgggcac
     4801 cgtggcgtag gacgcgcgca cggcggtctc ggccgcgccg ggtccccaca gccggggcag
     4861 cgtggggtac aggtggcggg ccaggtcgcg ctcggcgtcg cggatgcgct cgccgcagcc
     4921 gcgcagcagc cagtgcatca cgcggcagtc ggccggcacg ccgtcgacgg cgggcgggca
     4981 gggccgcccc cgctcgcaca ggtgctccag gcccatcacg tgcccgacct cgtgcagcag
     5041 cgtgcggcgc acgcggtcac cgcgctccgc cagcgggcac accgcgatgt ccacctcgga
     5101 ggtgaacacc gcgccggtgc cgccgcgcac gtccagcgtg gccaccgacg gccccagctg
     5161 ccagcgcacc gagccgagca gccccgcggg gcagcgcgtc cgcaggtaga ccgtggagcg
     5221 cccgtccggg tacgcctcgt ccagcgggcg gcgggacagg ccggcgaagc gcgtgcgcca
     5281 gcgcgacccc gtcacgccgt cccacgcgga caccgcctcg atcaccgtgc ccaggccgac
     5341 gcgccgcgcc agctcgcggt cgacctcgta ggtgaacggc agcgcgccgt ccgcgagcgc
     5401 cagccgccag tcctcgcggg ggccggccac cgtctgcacg cgcccgcgcg cccgcggcgg
     5461 gtccccggcc ggcgtggcca gcccgatggc ggccgccggg tacggccgga agggcgggcg
     5521 tggcccgtcc acccgcagtt cggcgtaggc cacccgccgc aggcgcgcca cgtcctccgc
     5581 gcgcggccgc ccgctgtcgt cggtgggggg cgccgcgaac agcaccgccg cggccagcag
     5641 cgccaccgac gcggccacga aacgcgcgct cacccgtgct cgcatcgccg gttgccctac
     5701 ccggcgggac gcgttacaga tcaccgccgc ggcgcccgaa tagcatgcgg gtgcttccca
     5761 tcccacacga ggagccacag cagtgagtca cggtcacggt ccaacccttt cggacgagcc
     5821 cggtctgggc atccccttct ccaggcgcga cgcgctggtg ccgcgcctgg tggtgcagcc
     5881 actgcagcgc ttcgcccaga cggagacctc ggcgggcgtg ctgctggcgc tcatggccat
     5941 cgtggcactg atctgggcca actccggctg gtacgagaac taccaccacc tctgggagac
     6001 cgagatcggc ctggacgtcg gcgggtgggg cctgcacctc acgctgcacg agtggatcaa
     6061 cgacggcgcg atggcgatct tcttcttcgt ggtgggcatg gagatcaagc gcgagctggt
     6121 gcacggccag ctgcgcgacc cgcgcgccgc cgcgctgccg gtgttcgccg cggtcggcgg
     6181 catggtcgtg ccggccctca tctacacggc gctcaacggg ggcgagccct cgtccaacgg
     6241 ctggggcatc cccatggcca ccgacatcgc gttcgccgtg ggcgcgctgt ccctggtcgg
     6301 caaacggctg ccgaccagcc tgaagatctt cctgctcacc ctggcggtgg ccgacgacct
     6361 cggcgcgatc gccgtcatcg cgatcttcta ctccaccggc gtgcaactgc agtggatcgg
     6421 catcgtggcg ctgaccgcgg ccgcggtgtg ggtcctgcag cgcatgcacg tgcgctggct
     6481 ggcgccgtac gtggtgctgg cgctgttcct gtggttcgcc acgcacgagt ccggcgtgca
     6541 cgccaccatc gccggcgtcg tgctgggctt cctcacgccc gcgcacccgt tccacccgcc
     6601 caagcgggcc gcccaggtgc tgcaccgggc actggatcgc gtgaccgcgg acaagcgggg
     6661 cgaggacagc gacgtcgacg agtacgagct gctcgacgtc gcccggctct cccgcgaggc
     6721 ggtgtcgccg ctggcccgcc tggagaacgc cctgcacccg tggtcggcgt acctcatcct
     6781 gccgctgttc gccctgtcca acgccggcgt gcgcgtggtc gactcggact tcggcgagct
     6841 gctcacgcgg ccggtcacgc tcggcgtgat gctgggactg gtggtcggca agccgctggg
     6901 cgtggtggcc gcctcgtgga tcgccacgaa gctgcgcatc ggcgtcctgc ccgccggcgt
     6961 cacctggccg cacgtcaccg cggtcggcct gttcgccggc atcggcttca ccgtggcgct
     7021 gttcgtgtcc ggcctgtcgt tcacggaccc cgacctgagc gacggctcca aggtcggcat
     7081 cctgctcgcc tc
//
LOCUS       DATMKC010000198         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_197371, whole genome shotgun sequence.
ACCESSION   DATMKC010000198 DATMKC010000000
VERSION     DATMKC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197371"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VNU01_05780"
     CDS             complement(<1..400)
                     /locus_tag="VNU01_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32164.1"
                     /translation="MPAAVAAVALAVLLSLLGAGCRAQDAPAGPEPSTAAAAPASPSP
                     SSPPAPVPGDAASAAARAEGGPPAVAADAGGLAAQLAAAEQRIRDESVPLAALAGDGH
                     LQQSAYRALARNPGWRDEVLARLPAHLRAPA"
     gene            complement(465..1286)
                     /locus_tag="VNU01_05785"
     CDS             complement(465..1286)
                     /locus_tag="VNU01_05785"
                     /inference="COORDINATES: protein motif:HMM:NF022740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HWH32165.1"
                     /translation="MARSARRLACRHAQRVRCAPPEVGAIPGLVAEHRVSGFSGEVVA
                     VEAGAVVLRDWRGRERAFPLAPGAFLVDDRPATLVRPRAAGTAPRVTASGAIAAPRAR
                     ARVAVPHRLFVEGVHDAELLEKVWGEELREAAVVVEPLHGADDLADAVRAFAPGPDRR
                     LGILLDHLVPGSKEERLAARVRAPHVLVAGHPWVDVWQGVRPRSVGLPSWPTVPRGTD
                     WKTGVCAALGAGDPPAFWRRLLGSVSTYADLEPQLVGAVERLLDFLIAQPSGPDG"
     gene            complement(1297..1473)
                     /locus_tag="VNU01_05790"
     CDS             complement(1297..1473)
                     /locus_tag="VNU01_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32166.1"
                     /translation="MTIRARALAAVLAVALLPACADNDQDGGDDAVGGAGTSANREDG
                     AANEPGREGYGDGS"
     gene            complement(1470..1628)
                     /locus_tag="VNU01_05795"
     CDS             complement(1470..1628)
                     /locus_tag="VNU01_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32167.1"
                     /translation="MGTGLIGIVVILVALAALAVWTRAHRGRADSGAKRGLPDDRPTT
                     WDPRRRYE"
     gene            complement(2072..>2654)
                     /locus_tag="VNU01_05800"
     CDS             complement(2072..>2654)
                     /locus_tag="VNU01_05800"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32168.1"
                     /translation="VDAGCALVLVLNKWDLVDDYRRDEIAKERERLLGFVQWAPLVRT
                     SALSGRHIDKLFPGIDRALDSWLTRIPTAKLNDWLADAVAATAPPMHNHRQVKLRYAT
                     QPAVGPPTVRVFATGEVPPGYLRYLERRLRERFDFTGSPIDLALKVRPRWEERAPEAA
                     SGGKRGGGQGGKGQGGKGQGGGSRKPGKSSRRG"
BASE COUNT          337 a         1024 c         1005 g          288 t
ORIGIN      
        1 gcgcgggggc gcgcagatgc gcgggcaggc gggccagcac ctcgtcgcgc cagcccgggt
       61 tgcgcgccag cgcgcggtag gccgactgct gcagatggcc gtccccggcc agtgccgcca
      121 gcggcacgga ctcgtcccgg atgcgctgct ccgcggcggc gagctgggcg gccagcccgc
      181 cggcgtccgc ggccacggcg gggggtccgc cctccgcgcg cgccgcggca ctcgccgcgt
      241 cgcccgggac gggcgccggc ggagacgacg gggaggggct cgcgggcgcc gccgcggcgg
      301 tggacggctc aggccccgcc ggtgcgtcct gggcgcggca gccggcgccc agcagggaca
      361 gcagcacagc cagtgcgacg gcggcgacgg cggcaggcat ggccccacgg taggcccgca
      421 ccgggtgcgg cgcagtgcgg ccacggtggg acggcgcgcc gcgcctagcc gtcaggaccg
      481 gacggctggg cgatgaggaa gtcgagcagc cgctccaccg cgccgaccag ctgcggttcc
      541 agatcggcgt aggtcgacac cgagccgagc aggcgccgcc agaacgcggg cgggtcccct
      601 gcgccgagtg cggcacacac gccggtcttc cagtccgtcc cgcgaggcac cgttggccac
      661 gacggcaggc cgacgctgcg cgggcgcacg ccctgccaca cgtccaccca cgggtgcccg
      721 gcgaccagga catggggcgc gcgcacccgc gcggcgagcc gctcctcctt ggagcccggc
      781 accaggtggt ccagcaggat gcccaggcgc cggtccggcc ctggcgcgaa ggcccgcacc
      841 gcatcggcga ggtcgtcggc gccgtgcagc ggctccacca ccacggcggc ctcgcgcagc
      901 tcctcgcccc acaccttctc cagcagttcg gcgtcgtgga cgccctccac gaacagccgg
      961 tgcgggaccg ccacgcgcgc gcgggcccgc ggcgcggcga tggcgcccga ggcggtcacc
     1021 cgcggagcgg ttccggcggc tcgagggcgg accagcgtgg ccgggcggtc gtcgacgagg
     1081 aaggcgcccg gggccagcgg gaacgcgcgc tcacggccgc gccagtcgcg cagcaccacg
     1141 gcgcccgcct cgacggcgac cacctcgccg ctgaagccgg acacgcggtg ctcggcgacc
     1201 aggccgggga tcgcgccgac ctccggcggc gcgcagcgaa ctcgttgagc atggcgacag
     1261 gctaagcggc gcgcggagcg cgccatcgca ggcgcgtcag ctgccgtcgc cgtagccttc
     1321 gcggccgggc tcgttggccg cgccgtcctc gcggttcgcg gacgtgccgg cgccgccgac
     1381 ggcgtcgtcg ccgccgtcct ggtcgttgtc ggcgcaggcg ggcagcagcg ccaccgccag
     1441 caccgcggcg agcgcgcggg cgcggatggt cattcgtatc gcctccgggg atcccacgtc
     1501 gtgggacggt cgtcgggcag gccgcgcttg gctccggaat cggcgcgtcc gcggtgcgcg
     1561 cgcgtccaca cggcgagggc ggccagcgcc accaggatca cgacgatgcc gatcagtccg
     1621 gttcccatgc ctcacccctt cccgcccagc gcggcggcca tgtcagccct ggcacgccta
     1681 cccgggaacg ccggcgatca tgagcgccgg cggcggagaa acggcagagg ggccgcgtcc
     1741 cccaacacgg cccctctgca tcggggaagg ctcacgaacc gggcccctcg gcgagccggc
     1801 acaaggccgc tcgccgcccc accaacaccc ccagcgcggc aggcatcgca accacgaggg
     1861 ctccaggagg gacccccgtc cgcagcggcc tgccgctgcg cgcgtccggc cggatccaca
     1921 gtcggcggaa cccgggcggc gatgcgctct tctgcagaga tgatggcgta ccaccgcgcg
     1981 gtacgggaac cccccgacgg gtgaatttcg ccaccccctc acccggaaat gcggggggtt
     2041 cgagcacgta cgccgggtaa tcggtgtggc gtcagccccg ccgggacgac tttccgggct
     2101 tcctggagcc gcccccctgg cctttgccgc cctggccttt gccgccctgg ccgccgccgc
     2161 gcttgccgcc ggaggcggcc tcgggggccc gttcctccca gcgcggccgg accttgagcg
     2221 ccaggtcgat gggcgagccg gtgaagtcga agcgctcgcg caaccgccgt tccagatacc
     2281 gcaggtatcc gggcggcacc tcgccggtcg cgaacacgcg caccgtcggg ggcccgaccg
     2341 ccggctgggt ggcgtagcgc agcttcacct ggcggtggtt gtgcatcggc ggcgcggtgg
     2401 cggccacggc gtcggccagc cagtcgttga gcttggccgt ggggatgcgc gtgagccagg
     2461 agtccagcgc ccggtcgatg ccggggaaca gcttgtcgat gtggcggccg gacagcgcgg
     2521 acgtgcgcac cagcggggcc cactgcacga agcccagcag gcgctcgcgc tccttggcga
     2581 tctcgtcgcg gcggtagtcg tccaccaggt cccacttgtt cagcacgagc acgagcgcgc
     2641 agccggcgtc gacc
//
LOCUS       DATMKC010000199         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_177660, whole genome shotgun sequence.
ACCESSION   DATMKC010000199 DATMKC010000000
VERSION     DATMKC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177660"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /gene="eno"
                     /locus_tag="VNU01_05805"
     CDS             complement(<1..1247)
                     /gene="eno"
                     /locus_tag="VNU01_05805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWH32169.1"
                     /translation="MEIEGLIARELLDSRGNPTVEVDVLLSDGAVGRAAVPSGASTGA
                     FEAVELRDGGDRYRGKGVREAVRNVHEVIAPALIGMDASDQRLVDQRLFELDGTENKS
                     ALGANAMLGVSLAVAKAAANSHELPLFAYLGGPNAHTLPVPMMNILNGGAHADSNVDF
                     QEFMIAPVGAPSFSEALRWGTEVYHALKEVLHDKGLGTGLGDEGGFAPNLDSNAAALD
                     LVMQAIQRAGYTAGEEIALAMDPATTELYRSNAYYLEGENRTLSSDEMVELWADLVAR
                     YPIVSIEDAMAEEDWDGWVALTSRLGDRVQLVGDDLFVTNVERVRRGIRLGAANSVLV
                     KVNQIGTLTETLDTMETAHRAGFTCMVSHRSGETEDTTIADLAVATNAGQIKTGAPAR
                     SDRVAKYNQLLRIEQILGDGARYA"
     gene            complement(1316..2473)
                     /gene="mazG"
                     /locus_tag="VNU01_05810"
     CDS             complement(1316..2473)
                     /gene="mazG"
                     /locus_tag="VNU01_05810"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HWH32170.1"
                     /translation="MPRLVLIDTSDQLPGLLPIHGWSALMSSELVLVADESHPFVPHL
                     DMAELRWEALPPLGEESVSLAGIDLMAGGDPRLVRRAKHVVRRVTDTDEAAYLFGPGD
                     GESFTQLLGMEAARAGIEVEVVYFAAVPKGLRLLELVKVEERLRGPGGCPWDREQDHR
                     TLAEYAVEEVFELVEAIESGDAAHIAEELGDVLLQVVFHAQIAEDAGGFTIDDVAGGI
                     ADKLVRRHPHVFGDTQVSGADEVVANWEELKAAEKPEREGVFDGVPAGQPALSYVRKL
                     QSRAAKTGFDWEADVEAAERIRLELEEFLAAGSDEQREHEIGDLLMSVVGLARRHKVD
                     PELALRGAARRFRERFEAMAAAADGPLEELSRQDWLALWERAKADQAQRES"
     gene            complement(2480..>2789)
                     /locus_tag="VNU01_05815"
     CDS             complement(2480..>2789)
                     /locus_tag="VNU01_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32171.1"
                     /translation="TEFGWHVIRVTGRTNPGLDEVADDIRTELTEQRRAEARTTFRQE
                     HLSGADVRVNPRLGRWNAETGEVEAVGPLGAAEPVEPDVGQLPLTPQGQDPAAPAPEQ
                     "
BASE COUNT          359 a         1058 c          959 g          413 t
ORIGIN      
        1 gcgtagcgcg cgccgtcgcc caggatctgc tcgatgcgca gcagctggtt gtacttcgcc
       61 acgcggtccg accgcgcggg cgcgccggtc ttgatctgcc cggcgttggt ggccacggcc
      121 aggtcggcga tcgtggtgtc ctcggtctcg cccgagcggt ggctcaccat gcaggtgaag
      181 ccggcgcggt gcgccgtctc catggtgtcc agcgtctccg tcagcgtgcc gatctggttc
      241 accttcacca gcacggagtt cgccgcgccc aggcggatgc cgcggcgcac gcgctcgacg
      301 ttggtgacga acaggtcgtc gcccaccagc tgcacgcggt cgcccagccg cgaggtgagc
      361 gccacccagc cgtcccagtc ctcctcggcc atcgcgtcct cgatcgacac gatcgggtag
      421 cgggccacca ggtccgccca cagctcgacc atctcgtcgc tggacagcgt gcggttttcg
      481 ccctccaggt agtaggcgtt ggaccggtac agctccgtgg tggccgggtc catcgccagc
      541 gcgatctcct cgccggcggt gtagccggcg cgctggatcg cctgcatgac caggtccagc
      601 gccgcggcgt tggagtcgag gttcggcgcg aagccgccct cgtcgcccag gcccgtgccc
      661 agccccttgt cgtgcaggac ctccttgagg gcgtggtaga cctcggtgcc ccagcgcagc
      721 gcctcgctga agctgggcgc gccgaccggg gcgatcatga actcctggaa gtcgacgttg
      781 gagtccgcgt gcgcgccgcc gttgaggatg ttcatcatcg gcacgggcag ggtgtgcgcg
      841 ttcggcccgc ccaggtaggc gaacagcggc agctcatggg agttcgcggc cgccttggcc
      901 accgccagcg acaccccgag catcgcgttg gcgcccagcg ccgacttgtt ctccgtgccg
      961 tccagctcga acagccgctg gtccacgagc cgctggtcgg aggcgtccat gccgatcagc
     1021 gccggcgcga tgacctcgtg cacgttgcgc accgcctcgc gcacgccctt gccgcgatag
     1081 cggtcgccgc catcacgcag ctccacggcc tcgaaggcgc cggtggaggc gccgctgggc
     1141 acggcggcac ggccgacggc gccgtcgctc agcagcacgt cgacctcgac ggtggggttg
     1201 ccgcgggagt ccagcaactc ccgcgcgatc aggccctcga tctccacgtc tgactcatcc
     1261 ctttcggcgt ctggtcactg tacgcaacag cagttcacat gcggaacagg cggcgtcagg
     1321 attctcgctg cgcctgatcg gccttcgcgc gctcccacag cgccagccag tcctggcgtg
     1381 acaactcctc cagggggccg tccgccgcgg ccgccatggc ctcgaagcgc tcccggaagc
     1441 gccgcgcggc gccgcgcagc gccagctccg gatccacctt gtggcgccgc gccagcccca
     1501 ccacggacat caagaggtcg ccgatctcgt gctcgcgctg ctcgtcggag cccgcagcca
     1561 ggaactcctc cagttccagc cggatgcgct cggccgcctc gacgtccgcc tcccagtcga
     1621 aaccggtctt ggcggcgcgc gactgcagct tgcgcacgta gctcagcgcc ggctgcccgg
     1681 cgggcacgcc gtcgaagacg ccctcgcgct cgggcttctc ggcggccttg agctcctccc
     1741 agttggccac gacctcgtcg gcgccggaga cctgcgtgtc gccgaacacg tgcgggtggc
     1801 ggcgcaccag cttgtcggcg atgccgcccg ccacgtcgtc gatcgtgaag ccgccggcgt
     1861 cctcggcgat ctgcgcgtgg aacaccacct gcagcagcac gtcgcccagc tcctccgcga
     1921 tgtgcgcggc atcgccggac tcgatggcct cgacgagctc gaagacctcc tccacggcgt
     1981 actcggccag cgtgcggtgg tcctgctcgc gatcccacgg gcagccgccc gggccgcgca
     2041 gccgctcctc caccttcacc agctccagca ggcgcaggcc cttgggcacg gcggcgaagt
     2101 acacgacctc cacctcgatg cccgcgcgcg ccgcctccat gccgagcagc tgggtgaacg
     2161 actccccgtc gccgggaccg aacaggtagg ccgcctcgtc ggtgtcggtg acgcgccgca
     2221 ccacgtgctt ggcgcggcgc accaggcgcg ggtcgccgcc ggccatgagg tcgatgccgg
     2281 ccagggacac ggactcctcg cccaggggcg gcagcgcctc ccagcgcagc tccgccatgt
     2341 ccaggtgcgg cacgaagggg tgcgactcgt cggcgaccag caccagctcg ctggacatca
     2401 gcgccgacca cccgtggatg ggcagcagcc cagggagctg atcggacgtg tcgatcagga
     2461 ccaggcgcgg cacgggcctc tactgctccg gcgcgggggc cgccgggtcc tggccctgcg
     2521 gcgtcagggg gagctggccg acgtcgggct ccaccggctc ggcggcgccc agggggccga
     2581 cggcctccac ctcgccggtc tcggcgttcc agcgccccag gcgcgggttg acgcgcacgt
     2641 ccgcgccgga caggtgctcc tggcggaagg tggtgcgcgc ctcggcgcgg cgctgctcgg
     2701 tgagctcggt gcggatgtcg tcggcgacct cgtccaggcc cgggttggtg cgcccggtca
     2761 cccggatgac gtgccagccg aactcggtc
//
LOCUS       DATMKC010000200         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_220178, whole genome shotgun sequence.
ACCESSION   DATMKC010000200 DATMKC010000000
VERSION     DATMKC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220178"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VNU01_05820"
     CDS             complement(<1..975)
                     /locus_tag="VNU01_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32172.1"
                     /translation="MPASGSTAIVEGLRLLRRVGEGGEGEVWEARDGAGRPRAFKLIR
                     PEALADPAQVAQRARQLRRIDHPALVRVHRSGILEAGRLAGWGFVEMDFVDGVPLDEA
                     PPDPHALERLLPLAEALDLLHAGEWSDGVPLVHRDVKPGNLIETPDGEVVLVDPSTLR
                     GLDSQVHTRIGTPLFCAPEVLRGKVTPAADVYSFAATLVALASGTRGGELGELLDDPS
                     GLDLPSGVARALSPRPSDRPRSCRAVLSAAEELTRVFPGADGWLAGAEEELDGGTVIL
                     RPGEPAGWVPHDGPVPAGSPLPWALALGALLVAPVATWGGTRPDALPMV"
     gene            1075..2097
                     /locus_tag="VNU01_05825"
     CDS             1075..2097
                     /locus_tag="VNU01_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWH32173.1"
                     /translation="MPPASPHQPRLPYAAGAAGALLIAFSAIFVRLADVSPATAAVFR
                     CVYALPALGLLALWERRRFGPRPWRRRVPAFGAGLFFSADLLLWHHSIHYIGAGLATV
                     LGNAQVVLVGLVAWVVLRERPGPNVLVAVPVVLAGGVLISGVVGTGAYGSDPARGAVY
                     GLLTAVAYTGFILLLRHSNADVRRPAGPLMDASAAGALGAAVGGLLAGDLSLVPAWPA
                     HGWLLALALGSQVVGWLCISVSLPRLPAAVTSVLLMLQPVGSVLLGMLLLAEAPSAVQ
                     LGGVVVVLAGVALASLPGRRPPALAGQDSASSGEPASPPSVEAASPGSSAPEPAGDSA
                     GADPRT"
     gene            complement(1980..>2251)
                     /locus_tag="VNU01_05830"
     CDS             complement(1980..>2251)
                     /locus_tag="VNU01_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32174.1"
                     /translation="LALLGLRRDAPQDCAILAIDPAAAQPEEGERVTVGLVRLRPPEG
                     DSVLQVVYVRGSAPAESPAGSGAEDPGDAASTEGGEAGSPDDAES"
BASE COUNT          219 a          920 c          824 g          288 t
ORIGIN      
        1 caccatcggc agggcgtccg ggcgcgtccc gccccaggtc gccaccggcg cgaccagcag
       61 cgcgccgagc gccagcgccc acggcagcgg cgagccggcc ggcaccgggc cgtcgtgcgg
      121 cacccatccg gcgggttcgc caggccgcag gatcaccgtc ccgccgtcca gctcctcctc
      181 cgcgccggcg agccagccgt cggcgccggg gaacacccgg gtcagctcct cggccgccga
      241 caggaccgcc cggcacgagc gcggccggtc cgacgggcgc ggggacaacg cgcgcgccac
      301 accgctgggc aggtccaggc cgctggggtc gtccagcagc tcgcccagct cgccgccgcg
      361 cgtcccggac gccagcgcca ccagcgtcgc cgcgaagctg tacacgtccg ccgcgggcgt
      421 caccttgccg cgcagcacct ctggcgcaca gaacagcggc gtgccgatgc gcgtgtgcac
      481 ctggctgtcc aggccccgca gcgtcgacgg gtcgaccagc accacctcgc cgtccggcgt
      541 ctcgatgagg ttgccgggct tcacgtcgcg gtgcaccagc ggcacaccgt cggaccactc
      601 cccggcgtgc agcaggtcca gcgcctccgc cagcggcagc agccgctcca gcgcgtgcgg
      661 gtcgggcggc gcctcgtcca ggggcacgcc gtccacgaag tccatctcca cgaagcccca
      721 gcccgccagc cggcccgcct ccaggatccc gctgcggtgc acccgcacca gcgccgggtg
      781 gtcgatgcgg cggagctggc gggcacgctg tgcgacctgc gccgggtcgg ccagcgcctc
      841 cgggcggatg agcttgaacg cccgcggccg gcccgccccg tcgcgcgcct cccagacctc
      901 gccttcgccg ccctcgccga cgcgccggag caggcgcagc ccctcgacga tggcggtgga
      961 gcccgatgcc ggcacgcccc ctccttcgtc gcggccatgt ccgacgtccg acgctatcgt
     1021 gccgtggacc cgtgccgtca cccgccaacc ccgtcacgac gcccgagagc ccgcatgcca
     1081 cccgcatccc cccatcagcc gcgcctgccg tacgccgcgg gggccgccgg cgcgctgctg
     1141 atcgccttct ccgccatctt cgtcaggctg gccgacgtca gccccgccac cgcggcggtg
     1201 ttccgctgcg tgtacgcgct gccggcgctg ggcctgctgg cgttgtggga acggcggcgc
     1261 ttcgggccgc gcccgtggcg ccggcgcgtg ccggccttcg gtgcggggct gttcttctcc
     1321 gccgacctgc tgctgtggca ccactcgatc cactacatcg gcgccggcct ggccacggtg
     1381 ctcggcaacg cgcaggtggt cctggtcggc ctggtcgcct gggtcgtgct gcgcgagcgc
     1441 ccggggccca acgtgctggt ggccgtgccg gtggtgctgg ccggcggcgt gctgatctcc
     1501 ggcgtggtgg gcaccggcgc ctacggcagc gacccggcgc ggggcgcggt gtacgggctg
     1561 ctgaccgccg tggcctacac cggcttcatc ctgctgctgc gccacagcaa cgcggacgtg
     1621 cggcggccgg cggggcccct gatggacgcg tcggccgccg gcgcgctcgg cgcggccgtg
     1681 ggcgggctgc tggccggcga cctgtcgctc gtgcccgcgt ggccggccca cggctggctc
     1741 ctcgccctgg cgctgggctc gcaggtggtg ggctggctgt gcatctccgt gtcactgccc
     1801 aggctgcccg ccgcggtgac gtccgtgctg ctgatgctgc agcccgtcgg ctcggtgctg
     1861 ctcggcatgc tgctgctggc ggaggcgccc tccgccgtgc agctcggcgg cgtcgtggtg
     1921 gtgctggcgg gcgtggctct ggcgagcctg cccggacggc gcccgcccgc cctcgcgggt
     1981 caggactcgg cgtcgtccgg cgagcctgcc tcgccgccct ccgtggaggc cgcgtcaccg
     2041 gggtcctccg cgcccgagcc ggcgggcgac tccgccggcg cggacccgcg cacgtagacc
     2101 acctggagca cgctgtcgcc ctccggcggg cgcagccgca ccagcccgac ggtcacgcgc
     2161 tcgccctcct cgggctgcgc cgcggccggg tcgatcgcca ggatcgcgca gtcctgcggc
     2221 gcgtcgcggc gcagcccgag gagggcgagc g
//
LOCUS       DATMKC010000201         3574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_264472, whole genome shotgun sequence.
ACCESSION   DATMKC010000201 DATMKC010000000
VERSION     DATMKC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264472"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1447
                     /locus_tag="VNU01_05835"
     CDS             131..1447
                     /locus_tag="VNU01_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32175.1"
                     /translation="MAVALLLAVLTLLPNGVQADLLDGAGLELPALSEVSSGWMHSCG
                     LGQDGKVYCWGNNATGNLGDGTAANARPLAKRVDGMDAPAIQVSSGADHSCALTTNGD
                     AWCWGGNLSGQLGVNSDTLVLRRQPGKVVDGYRGIALPRFVQISAGGSHSCALAENGV
                     PWCWGANDVGQLGSTPPSAILPDDGGDARPVWTSQMGTATKIIAGAAHTCAINTVSEL
                     WCWGDNGAGQLGVNSKQEAWSPVRVHATGGFPDGGVATVALGTTHTCASTRNGNAYCW
                     GDNSYGRIGSTSPTSDTAQGPDSLVPQKVRPYPGSRAETGIGGNANMRFADLMGVSAG
                     LDHSCAWTRTGVGWCWGRAAYGHLGDGTVSASQPKPRFQVVEYTLQFVDTGAIQHISA
                     GYNHTCSLNANGRAHCWGHNGFGQVGDGTFVFHHRPAKVLQLDAPV"
     gene            complement(1451..2266)
                     /locus_tag="VNU01_05840"
     CDS             complement(1451..2266)
                     /locus_tag="VNU01_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32176.1"
                     /translation="MDTALARSLHRVVEPLHAWVYLTPESKAVYADAGLKGQWMGYFA
                     SRSAAMGAVRPAVVEAAFFGFAPALVRRAIPDAWTLSTPERVVQARLELVDQGLRRLL
                     GDLVDAPELKEAAALARAAADAADCAGRPLAAAHQALADTGLPHVDLWQAITVLREHR
                     GDGHLAALLTAGLDGREALLTVAAIKPSYRDFVHNLRGWTAEELAESTQRLRERGLVD
                     ADGDGTPACQELRARIEDDTDRLALQPYRVLGQERSERLRDALAPLAERIRNG"
     gene            2306..2974
                     /locus_tag="VNU01_05845"
     CDS             2306..2974
                     /locus_tag="VNU01_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32177.1"
                     /translation="METTPVLLSWSGGKDSMMSLLALREDPAVEVAGLLTSVTSGYER
                     VSVHGVRRELLDAQAAALGLPVVEIHLAPESSNAAYEEAFAAAVAAPLARGVRTVAFG
                     DLFLADVRAYREALLDRLGLDGLYPVWGRDTAAFARDVVARGVRAVLCAVDTGRLDAA
                     FAGRDYDEALLEELPEEVDPAGERGEFHTFVWDGPGFAAPVAIRRGKVEDRPPMRYTD
                     LLAG"
     gene            complement(2998..3366)
                     /locus_tag="VNU01_05850"
     CDS             complement(2998..3366)
                     /locus_tag="VNU01_05850"
                     /inference="COORDINATES: protein motif:HMM:NF040913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SaoD/DsrE family protein"
                     /protein_id="HWH32178.1"
                     /translation="MKVAYVFSTSGHTASYKLGKMILPQLEEGRHGVEVIGMMFFDDN
                     NFVLRKGDPIGERLSKIAGDTGMLLMMCDQCAIERGLAVGEPGNCKVADTVVDNVQVG
                     CFPDLYAALAGNPPDHVITL"
BASE COUNT          491 a         1300 c         1319 g          464 t
ORIGIN      
        1 gtcgcgtaag cacacggggg gacccggacg tacgaaaaga tcatccggct tatcggattt
       61 tcttgcgggc gagcgggcgc ggaggcgacg gacaatggcg cgtgtggcga aaatgagggc
      121 tccggcggcg gtggccgtgg cactcctcct ggccgtgctc accctgctgc ccaacggcgt
      181 gcaggccgac ctgctcgacg gcgcgggcct ggaactgccc gcgctctcgg aggtcagctc
      241 cgggtggatg cacagctgcg gcctcggcca ggacggcaag gtgtactgct ggggcaacaa
      301 cgccaccggc aacctcggtg acggcacggc cgccaacgcc cgcccgctcg ccaagcgcgt
      361 cgacggcatg gacgccccgg cgatccaggt ctcctccggc gccgaccaca gctgcgcgct
      421 caccacgaac ggcgacgcgt ggtgctgggg cggcaacctg tccggccagc tcggggtgaa
      481 ctcggacacg ctggtgctgc gccgccagcc cggcaaggtc gtggatggct accgcggcat
      541 cgcgctgccg agattcgtcc agatcagcgc cggcggctcg cacagctgcg cgctcgcgga
      601 gaacggcgtg ccgtggtgct ggggcgccaa cgacgtcgga cagctgggca gcacgccccc
      661 cagcgccatc ctgcccgacg acggcggtga cgcccggccc gtgtggacca gccagatggg
      721 cacggccacc aagatcatcg cgggcgcggc ccacacctgc gcgatcaaca ccgtctccga
      781 gctgtggtgc tggggcgaca acggcgcggg acagctcggc gtgaacagca agcaggaggc
      841 ctggtccccc gtgcgcgtgc acgccaccgg cggcttcccc gacggcggtg tcgcgaccgt
      901 ggccctgggc accacgcaca cctgcgcgtc cacgcgcaac ggcaacgcct actgctgggg
      961 cgacaacagc tacggccgca tcgggtccac cagcccgacg agcgacacgg cgcagggacc
     1021 cgactccctg gtgccgcaga aggtgcggcc ctacccgggc agccgcgcgg agacgggcat
     1081 cggcggcaac gcgaacatgc gcttcgccga cctgatgggc gtctcggccg gcctggacca
     1141 ctcctgcgcg tggacgcgca cgggcgtcgg ctggtgctgg ggcagggcgg catacggaca
     1201 cctcggcgac ggcacggtct cggccagcca gccgaagccg cgcttccagg tcgtggagta
     1261 cacgctgcag ttcgtcgaca ccggcgcgat ccagcacatc tcggccggct acaaccacac
     1321 ctgctcgctg aacgccaacg gcagggcgca ctgctggggc cacaacggct tcggccaggt
     1381 gggcgacggc accttcgtct tccaccaccg cccggccaag gtcctccagc tcgacgcgcc
     1441 ggtgtgacgc tcagccgttc ctgatccgct cggccagcgg ggccagcgcg tcgcgcaggc
     1501 gctcgctgcg ctcctggccc agcacccggt agggctgcag cgccagccgg tcggtgtcgt
     1561 cctcgatgcg ggcgcgcagc tcctggcagg ccggcgtccc gtcgccgtcg gcgtccacca
     1621 gtccgcgctc gcgcaggcgc tgcgtggact cggccagctc ctcggcggtc cagccgcgca
     1681 ggttgtgcac gaagtcgcgg taggacggct tgatcgcggc cacggtcagc agcgcctcgc
     1741 ggccgtccag cccggcggtg agcagggcgg cgagatgccc gtcgccgcgg tgctcgcgca
     1801 gcaccgtgat ggcctgccac aggtccacgt gcggcagccc ggtgtccgcc agcgcctgat
     1861 gggcggccgc cagcggccgg cccgcgcagt cggccgcgtc ggcggccgcg cgcgcgagcg
     1921 ccgccgcctc cttgagctcg ggggcgtcca ccaggtcccc gagcaggcgg cgcagcccct
     1981 gatcgaccag ctccagccgc gcctggacga cgcgttccgg cgtggacagc gtccacgcgt
     2041 ccgggatcgc ccggcgcacc agcgcggggg cgaagccgaa gaacgcggcc tcgacgacgg
     2101 ccggccgcac ggcccccatc gcggccgagc gggaggcgaa gtagcccatc cactggccct
     2161 tgaggccggc gtcggcgtag accgccttgc tctccggcgt caggtacacc caggcgtgga
     2221 gcggttcgac gacgcgatgc agggagcgag ccagcgcagt gtccatttca tgatgctacc
     2281 aacgcaggcg ctagcctgcg cggccatgga gacgaccccg gtactgctga gctggagcgg
     2341 cggcaaggac tcgatgatgt ccctgctcgc cctgcgcgag gacccggcgg tcgaggtcgc
     2401 cgggctgctc acgtccgtga cgtcgggcta cgagcgcgtc agcgtgcacg gcgtgcgccg
     2461 cgagctgctg gacgcgcagg cggccgcgct gggcctgccc gtggtggaga tccacctggc
     2521 gccggagtcg tcgaacgcgg cctacgagga ggccttcgcc gcggccgtcg cggctcctct
     2581 ggcccgaggc gtgcgcaccg tggcgttcgg cgacctgttc ctggccgacg tgcgcgccta
     2641 ccgcgaggcg ctgctggacc ggctggggct ggacggcctg taccccgtct ggggacgcga
     2701 cacggcggcg ttcgctcgcg acgtggtggc ccgcggcgtg cgcgcggtgc tctgcgccgt
     2761 ggacaccggc cggctcgacg ccgccttcgc cggccgcgac tacgacgagg cgctcctgga
     2821 ggagttgccg gaggaggtcg accccgcggg cgagcgcggc gagttccaca cgttcgtctg
     2881 ggacgggccg ggcttcgccg cgccggtggc gatccggcgc ggcaaggtcg aggaccgccc
     2941 gcccatgcgc tacaccgacc tgctcgccgg gtaggaccgc cgcgcctgct cggccgccta
     3001 gagcgtgatg acgtggtccg gcgggttgcc ggccagcgcc gcgtagaggt cggggaagca
     3061 gccgacctgg acgttgtcga ccacggtgtc ggcgaccttg cagttgcccg gctccccgac
     3121 ggccaggccg cgctcgatcg cgcactggtc gcacatcatg agcagcatgc cggtgtcgcc
     3181 ggcgatcttc gacaggcgct cgccgatcgg gtcgcccttg cgcagcacga agttgttgtc
     3241 gtcgaagaac atcatgccga tgacctcgac gccgtgccgg ccctcctcga gctgcgggag
     3301 gatcatcttg ccgagcttgt agctggcggt gtggccggac gtggagaaga cgtaggcgac
     3361 cttcacgatg gcactccttg tggcgcgcgc ggcgcggcgg cggctgcttg ctgagatccg
     3421 ccagcctaga ggacccgggc aggcgcgccg gacgggcggc cggtcagcag caggcgtcgc
     3481 cggccgacgg ggcggcggag acggtcgcca gcaggccgaa ctgcccctcg gttggctcgg
     3541 cgcagcagaa ccacggctcg ctcaggcccg gcgc
//
LOCUS       DATMKC010000202         5543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_173252, whole genome shotgun sequence.
ACCESSION   DATMKC010000202 DATMKC010000000
VERSION     DATMKC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173252"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VNU01_05855"
     CDS             complement(<1..430)
                     /locus_tag="VNU01_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HWH32179.1"
                     /translation="MHDAVLVALGFGSAAVGGVGGLGGAIFLVPLLALLGMEPLAAAP
                     LGLLAVTAGSLAAGGSQLAEGLTHHRLGVVTELPASAGAVAGAFLALHVPAAVLLRVL
                     AAGALGAAVAGLTRRGMRNRPEPLFAAERPGEWPGTLAGAY"
     gene            545..1807
                     /locus_tag="VNU01_05860"
     CDS             545..1807
                     /locus_tag="VNU01_05860"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWH32180.1"
                     /translation="MPRRRSRLIAPLALTLALALVSLVPPGALAQEERELYDVRLFAQ
                     IGMPGQPEGIAVGRDGTVYVGTHNYGKGDAEAPSKIFAYSPAGELRREYVIQGQNLDE
                     DHGVLQFALDARDTLYVLDRNPPRLFTLDPRTGAQRDYATFDDVRPCGGGGEPGHCSA
                     GSLDLAPFADYPVFAPDGTLYVTDLEQGLIWRVPRGGGEAEVWFTDPRLEGVFGPNGI
                     QFMADGETLLFAQTGSAPPGASDLGQGVLYTLPVNPDGSPGELAEFWKGRPVDGPDGF
                     AIGASGKVYVGLAGASQFVVLSADGEELARFPETPLHNTQQTPPFDTPASLAFHGERV
                     LMTNQSFFTGTESGWTVADIFVGETGLPLYRPAMPAIDTVQPTTTPAPGVPASEGLPA
                     TGAGGVAAAAAGLLLLGSGLLAVRRRPS"
     gene            1844..2794
                     /locus_tag="VNU01_05865"
     CDS             1844..2794
                     /locus_tag="VNU01_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWH32181.1"
                     /translation="MSTTAEGLQAPQDTPSAVGSSPPEDDGRLRLSFSRVDAYRQCPL
                     KFRFAYLDKLPSTPSPELSWGSSIHAALEAWWDQKLPEPPPVEFLWQALFDHWDDEGF
                     EGMAREDKLKWYQHARNVLAQHHQRHAPSFQPAVATEEWFELDLGDDIVVLGSIDHVA
                     RTPSGGIGIVDWKTNRKAKNRKQVAGSLQLAIYTLAARELWGQDPEWVALDFVVPGVR
                     VTVGREEIDTDAALAAVRETARLVRAELFEPKPSALCPWCDYRALCPAFEGDGPDVAG
                     TALVELKRLRRKAARDAERMAHLERLVAERLGPDALLDLS"
     gene            complement(2879..3260)
                     /gene="rnpB"
                     /locus_tag="VNU01_05870"
     ncRNA           complement(2879..3260)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNU01_05870"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3339..4121)
                     /locus_tag="VNU01_05875"
     CDS             complement(3339..4121)
                     /locus_tag="VNU01_05875"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWH32182.1"
                     /translation="MVEAFFEPLGDGRFWATENTLGPWGGGSQHGGPPCALLGRALER
                     HEARDGMEVVRFTAEILRPVPQAEVAVSVRTARGGRNVELLEATLAAADGTELVRASA
                     WRMNLQRDGFAAGDAGPPMPGPDQGAPHPFFDVGLETGYHTGMEWRFVEGAFLEPGPA
                     KVWLRMRVPLVAGEAPTPLQRVLCAADSGNGVSATADARTLLFINTDLTVHLRRPLRG
                     EWVGMDARTVIEADGVGLAESVLHDTDGPIGRGAQALFVAPR"
     gene            4309..4512
                     /locus_tag="VNU01_05880"
     CDS             4309..4512
                     /locus_tag="VNU01_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWH32183.1"
                     /translation="MAVGTVKWFSDDKGFGFISQEGGEDVFVHYSAINSNGFKTLAEG
                     ARVEFDITQGPKGAQAANVQIVG"
     gene            complement(4599..4895)
                     /locus_tag="VNU01_05885"
     CDS             complement(4599..4895)
                     /locus_tag="VNU01_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWH32184.1"
                     /translation="MKVEPSPSRGFALVEYELAPGSGPGQHAHEECHETYYILEGEVV
                     FRLGRDMHVLTAGGLLHVPSGAVHGFRNETGEPARLLWLFDPGHQFHAMGWDQR"
     gene            complement(5104..>5543)
                     /locus_tag="VNU01_05890"
     CDS             complement(5104..>5543)
                     /locus_tag="VNU01_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32185.1"
                     /translation="ADDEDTTALAGLLERLVDGAEAYGRPLFAGWRRVPRCGDGPGRV
                     NQALSTLREQRGGSHDIAILAQGLRPVEAMAARGELGLQSAGYFGWPPPYPDGAPFRA
                     RWEEAERVTDALAAPVMEALTASERERLASLVARALEQAQAAS"
BASE COUNT          761 a         2095 c         1963 g          724 t
ORIGIN      
        1 ggtaagcgcc ggcgagcgtg cccggccact cccccgggcg ctcggcggcg aacagcggct
       61 ccgggcggtt gcgcatgcca cggcgggtca gccccgcgac ggcggccccg agcgcgccgg
      121 cggccagcac ccgcagcagc acggcggccg ggacgtgcag cgccaggaac gcgccggcca
      181 ccgcgcccgc gctggccggc agctccgtca ccacgcccag gcggtgatgg gtcagccctt
      241 cggcgagctg gctgccgccc gccgccaggg agccggccgt cacggcgagc aggcccaggg
      301 gggcggcggc gagcggctcc atgcccagca gggccagcag cggcaccagg aagatggcgc
      361 cgcccagccc gcccacgccg ccgacggcgg cgctgccgaa gcccagggcc accagcaccg
      421 cgtcgtgcac gcaccgagcc tttccatcgc gggcccatcc cgggcgacgt ccctgcgact
      481 acgatacgca ggccgcgagg cggggcgcgg ccgcaccggt gggacatcga acgggagaag
      541 cgccatgccg cgtcgccgca gccgactgat cgctccgctc gccctgacac tggccctggc
      601 actcgtgtcg ctggtgcctc ccggcgcgct ggcgcaagag gaacgcgagc tgtacgacgt
      661 gcggctgttc gcgcagatcg gcatgcccgg ccagccggag ggcatcgcgg tgggccgcga
      721 cggcaccgtg tacgtcggca cccacaacta cggcaagggc gacgccgagg ccccgtccaa
      781 gatcttcgcg tactcgccgg ccggcgagct gcggcgcgag tacgtgatcc agggtcagaa
      841 cctcgacgag gaccacggcg tcctgcagtt cgcgctggac gcccgcgaca cgctgtacgt
      901 gctggaccgc aatcccccgc gcctgttcac gctggacccg cgcaccggcg cgcagcgcga
      961 ctacgcgacc ttcgacgacg tgcgcccgtg cggcggcggc ggcgagccgg gccactgctc
     1021 cgccgggtcg ctggacctgg cgccgttcgc ggactatccc gtgttcgcgc cggacggcac
     1081 gctgtacgtc accgacctgg agcaaggact gatctggcgc gtgccgcgcg gcggcggcga
     1141 ggcggaggtg tggttcaccg acccccgcct ggagggcgtg ttcggcccca acggcatcca
     1201 gttcatggcc gacggcgaga cgctgctgtt cgcccagacc gggtccgccc cgccgggggc
     1261 gtccgacctc gggcagggtg tgctgtacac cctgccggtg aacccggacg gctcgccggg
     1321 cgagctggcg gagttctgga agggccggcc ggtggacggc cccgacggct tcgcgatcgg
     1381 cgcgtccggc aaggtctacg tggggctggc cggcgccagc cagttcgtgg tgctgtcggc
     1441 ggacggcgag gagctggcgc gcttcccgga gacgccgctg cacaacaccc agcagacgcc
     1501 gccgttcgac acgccggcca gcctggcgtt ccacggcgag cgcgtgctca tgaccaacca
     1561 gtcgttcttc accggcacgg agtccggctg gaccgtggcg gacatcttcg tgggcgagac
     1621 cgggttgccg ctgtaccggc ccgccatgcc ggcgatcgac accgtccagc ccacgacaac
     1681 ccccgcgccg ggcgtgcccg ccagcgaggg gctcccggcc accggcgcgg gcggcgtggc
     1741 ggccgccgcc gccggcctgc tgctgctggg ttccgggctg ctggccgtca ggaggcgccc
     1801 gtcctgacgg cggggtgtcg caggcagggg ttaccctgcc tgcatgagca ccaccgcgga
     1861 gggccttcag gcgcctcagg acacgccgtc tgccgtcggg tcgtcgcctc ctgaggacga
     1921 cgggcggctg cgcctgtcgt tctcccgcgt ggacgcctac cggcagtgcc cgctcaagtt
     1981 ccgcttcgcc tacctggaca agctgccgtc cacgccgagc ccggagctgt cctggggctc
     2041 gtcgatccac gccgccttgg aagcctggtg ggaccagaag ctgcccgagc cgccgccggt
     2101 ggagttcctg tggcaggcgc tgttcgacca ctgggacgac gagggcttcg agggcatggc
     2161 ccgcgaggac aagctcaagt ggtaccagca cgcccgcaac gtgctggccc agcaccacca
     2221 gcggcacgcg ccctcgttcc agccggccgt ggccaccgag gagtggttcg agctggacct
     2281 gggcgacgac atcgtggtcc tgggctccat cgaccatgtg gcgcgcacgc cgtccggcgg
     2341 catcggcatc gtggactgga agaccaaccg caaggccaag aaccgcaagc aggtggccgg
     2401 cagcctccag ctcgcgatct acacgctggc ggcgcgcgag ctgtggggcc aggacccgga
     2461 gtgggtcgcg ctggacttcg tggtgccggg cgtgcgcgtc accgtgggcc gcgaggagat
     2521 cgacaccgac gccgcgctgg cggcggtccg cgagaccgcg aggctggtgc gcgcggagct
     2581 gttcgaaccg aagccctcgg cgctgtgccc gtggtgcgac taccgcgcgc tgtgcccggc
     2641 gttcgagggc gacgggccgg acgtggcggg caccgcgctg gtggagctga agcggctgcg
     2701 ccgcaaggcg gcgcgcgacg ccgagcgcat ggcgcacctg gagcgcctgg tggccgagcg
     2761 cctcggcccc gacgccctcc tcgacctctc ctgaccttcc ccgttcaaac gggtgcgggc
     2821 accgcgacat accgcagtcc ccgcacccgt tcgaatgggg aacgaacggg gatgggtgtc
     2881 ggggagccgg ccgataagcc ggattctgtc ctcactcgcg tgagtggcga ccatctctct
     2941 ggggccgacg ttgccgccga cctcgatgcg gcctacctgg cactcggcgg gaccacctga
     3001 tgacgtgcct gcttggcctt gctccggacg gggtttacct ggccgccggc gtcaccgccg
     3061 gcgccggtgc gctcttaccg caccctttca ccctcaccac gcgcccgccc caggtctccc
     3121 tcgggcgggc cgttcggcgg tctgctctct gtggcacttt ccgcgcgtcg ccgcgcctgg
     3181 gcgttaccca gcgtcctgcc ctgtggagtc cggactttcc tcagcgaggt tgccctcacc
     3241 gcggccgcct ggccgactcc ccgacacccc agggtaccgt gaggacccgg cgccgcccga
     3301 gggtcaccgg ggagcgacga accgcaccac gcgccgagct aacgaggtgc gacaaacaag
     3361 gcctgagcgc cgcggccgat cgggccgtcg gtgtcgtgca gcaccgactc agccagcccc
     3421 acgccgtcgg cctcgatgac cgtgcgcgcg tccatgccca cccactcgcc gcgcagcggc
     3481 cggcgcaggt gcaccgtgag gtcggtgttg atgaacagca gcgtgcgggc gtccgccgtc
     3541 gcgctcacgc cgttgccgga gtcggccgcg cacagcaccc gctgcagcgg cgtgggcgcc
     3601 tcgccggcca cgagcggcac gcgcatgcgc agccacacct tcgccgggcc cggctccagg
     3661 aacgcaccct cgacgaagcg ccactccatg cccgtgtggt agccggtctc caggcccacg
     3721 tcgaagaacg ggtgcggcgc cccctggtcc ggcccgggca tgggcggccc cgcgtcgccc
     3781 gcggcgaagc cgtcgcgctg gaggttcatg cgccaggccg acgcgcggac cagctcggtc
     3841 ccgtccgcgg cggcgagcgt cgcctcgagc agctcgacgt tcctgccgcc gcgcgccgtg
     3901 cgcaccgaca ccgccacctc cgcctgcggc acggggcgca ggatctcggc ggtgaagcgc
     3961 accacctcca tgccgtcgcg cgcctcgtgg cgctccagcg cgcggccgag caacgcgcag
     4021 ggcggcccgc cgtgctggct gcccccgccc caaggcccca gggtgttttc cgtcgcccag
     4081 aagcggccgt cccccagcgg ctcgaagaac gcctccacca tggcctcctg tcgcctcctt
     4141 acgggcgaat caaatcgccc ctgttcagca ccttcccgac tggtagcctt tcaggtcgag
     4201 ggcacgaagt acgtgacgtc tgcagtggtg caacaccagg gcagcccctc agggtgggat
     4261 cggaaatcct tcgatcacac ccgaaagcac aaggaagagg gcagcaccat ggctgttggc
     4321 accgtgaagt ggttctccga cgacaagggc ttcggcttca tttcgcagga gggcggcgag
     4381 gacgtcttcg tccactactc cgcgatcaac tcgaacgggt tcaagacgct cgccgaaggc
     4441 gcgcgtgttg agttcgacat cacgcagggc cccaagggcg cccaggcggc gaacgtccag
     4501 atcgtcggct agtccactcg gacgccaagt ctgaacacga aggccccgga gcgatccggg
     4561 gccttcgcgc tgttccgggg cctgtccgtg ccgcgccgct agcgctggtc ccagcccatc
     4621 gcgtggaact ggtggccggg atcgaacagc cacagcagcc gggccggctc cccggtctcg
     4681 ttgcggaacc cgtgcacggc cccggacggg acgtgcagca gccccccggc cgtgagcacg
     4741 tgcatgtcgc ggcccaggcg gaacaccact tcgccctcga ggatgtagta ggtctcgtgg
     4801 cactcctcgt gggcgtgctg ccccgggccg ctgccgggcg ccagctcgta ctcgacgagc
     4861 gcgaaccccc tcgacgggga cggctcgacc ttcacggtca cacgatcctc accgacgcgc
     4921 agcactcggc cttgaccggg cggatggacc acttccttcg gcacgctgcc tcgacccccg
     4981 ttgatggaac gccagggccg ccgggccagc aatgaaagtg gcgtcggtca gcccggcaaa
     5041 cgttgacagc ctaaccaatc ccccgcgatg cgcaagccag gcggctccac gcgtcccggt
     5101 gacctatgag gcggcctgcg cctgctcgag cgcgcgcgcc accagcgacg ccagccgctc
     5161 gcgctcggac gcggtgaggg cctccatgac cggggccgcc agcgcgtccg tgacccgctc
     5221 cgcctcctcc cagcgggcgc ggaagggcgc gccgtccggg tagggcggag gccagccgaa
     5281 gtagcccgcg ctctgcaggc ccagctcgcc gcgggcggcc atggcctcca cggggcgcag
     5341 cccctgggcc aggatcgcga tgtcgtgcga gccgccgcgc tgctcgcgca gcgtgctgag
     5401 cgcctggttc accctgccgg ggccgtcgcc gcagcggggg acccgccgcc agccggcgaa
     5461 cagcggccgg ccgtacgcct ccgcgccgtc cacgaggcgc tccagcaggc cggccagcgc
     5521 ggtggtgtcc tcgtcgtcgg cgt
//
LOCUS       DATMKC010000203        11309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_113058, whole genome shotgun sequence.
ACCESSION   DATMKC010000203 DATMKC010000000
VERSION     DATMKC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113058"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..555
                     /locus_tag="VNU01_05895"
     CDS             154..555
                     /locus_tag="VNU01_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWH32186.1"
                     /translation="MPKRRNHLDGLLGPLETEVMEACWELGDTTVRDVHEKLAKRLDL
                     AYTTIMTTMTRLAGKGLLKRDTTGLAHRYRPALSRDEYASSTVHNVVEWLVNSFPEPA
                     MSYFAQVVGDGDTGELEALRDRIDRLRDQEA"
     gene            555..2030
                     /locus_tag="VNU01_05900"
     CDS             555..2030
                     /locus_tag="VNU01_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HWH32187.1"
                     /translation="MPLDGSGGFLLSLLTESFAVRALIGSLAAALLALLVVRRDVVRH
                     RTARRVTVLAPVLVAAGAAVAVAREGGAYLPQLWVAGGRGPAGEILELLGEWRVFVPG
                     SGVDVLVGGYLVCAGVLLGRRVAGARRLRRLLAGAIEPVGYGRLMPALHRLSAKVGVR
                     APRLVLLEDCPGGAFTAGLRRPVLALDPVLVDRLDDRELEGLLAHELAHLRRRDNHVG
                     LAVGLFRDLVFFLPPVHLAQRWLRREQEESADELASEHTGRPVALASSILKVWDRHRE
                     RPPAWRPRLACSALGTAEAAPAAARDALALRVERLLEGGARALSPRRRRLEIALAMAL
                     LVAGGLAAWVVPGWISRDLNAYSLSFIYLTAPAAGEVEAPAFATFRALTSEASARPGL
                     RSGQAMAAPGPSGEAASGERAVRDPALCPCVESVAQLRAGEVFEADPPSPRMLWREAG
                     HDAWQLSRLRERGGLHSRPLLTLTEGGPQMGFFVVGRPPAS"
     gene            2123..2353
                     /gene="secG"
                     /locus_tag="VNU01_05905"
     CDS             2123..2353
                     /gene="secG"
                     /locus_tag="VNU01_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWH32188.1"
                     /translation="MLTPILVVLHIIVSLILIMLILLKSGRGGGLSDMFGGGAITSMQ
                     GSTVVEKNLDRLTVVIGVLFGVTTIVLALRLV"
     gene            2358..3950
                     /locus_tag="VNU01_05910"
     CDS             2358..3950
                     /locus_tag="VNU01_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH32189.1"
                     /translation="MRRARRTTLLLVALALAAACDPGAAPPPAAPSPAGATSAPPSAT
                     LRVAIDRPDALLPQRADEQAEQWLADAVFDSLTRWGPDLRPVPAAAVSWSADASQRVW
                     TFRLREDAAWHDGRPVLAADFVRGWRALVRAGRMAHLLFDVEGVDGTDPARAEPAVRA
                     LDARTLEVRLRRPSADFPAVAAHPALAPLPPGADSAEYAARPVGNGPFAVPEAGASSR
                     FVRVGRFPGWRNGPAPALDEIVFQVMDPETAFLAFQQQRVHLSPVPPGALTALLTESP
                     GSAWRGERAVLYLLGMDHTRPPFDRVEARRALSLAVDRVALAAAVSEGAAAPAGGLLP
                     AWLPEAVPERCGSCVRDPGAARELFAQAGVTALRLSFNTGAGHETVARRVRDDLAEAG
                     VALEFDARPGPEFLAAARAGELTLFRLGWQPDVPVAGAVLRPLLHGDAVPSGPEGVRH
                     NLGRYRAADVDALLDQARQTAAPLVRAATYRRAARLALERDQAVVPLFVSRHRLARAP
                     EAAGLAPDPMGQVDWAAVRLER"
     gene            complement(3947..4978)
                     /locus_tag="VNU01_05915"
     CDS             complement(3947..4978)
                     /locus_tag="VNU01_05915"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWH32190.1"
                     /translation="MSVAASGADLHRRAAAGEEVLRVEGLVKHFPVTGGLLKRQVATV
                     HAVEGVDLALHAGETLGLVGESGCGKSTLGRMVVRLLEPTAGRILFKGEDVTNWRGKR
                     LRELRREVQIVFQDPYASLNPRMTVRDIVAEPLRIHGEWTSGGGERRVAELLRLVGLS
                     PEHGNRFPHEFSGGQRQRIGIARALALDPQVLVLDEPVSALDVSIQAQIVNLLRELQD
                     ELGLAYLFIAHDLSVVRHISDRVAVMYLGRIMEQGERPQVYRRPTHPYTQALLSAVPI
                     PDPALRGRRERIVLSGDVPSPVSPPSGCVFRTRCWKAQDRCAQEVPELADRFGHGHPA
                     ACHYAETRA"
     gene            complement(4975..6027)
                     /locus_tag="VNU01_05920"
     CDS             complement(4975..6027)
                     /locus_tag="VNU01_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32191.1"
                     /translation="MNVREPVLSVRDLVVEFATPDGVVHAVAGMDYDLYPGETLAIVG
                     ESGSGKSVSAMALLGLVPQPPGRIASGSARLRGRELLGLRGRELRQIRGREIAMVFQD
                     PMTSLNPVLRIGEQIAEALAVHDPGGSAGERARRVVELLELVGVPDARERAAQYPHEY
                     SGGMRQRAMIAMAMANRPAILVADEPTTALDVTIQAQVLDVLRAAQKETEAATILITH
                     DLGVVAELADRVAVMYAGRIVEMADAETLFARPRHPYTLGLLASLPRLDRDQERLSPI
                     PGQPPSLIDVPSGCPFHPRCRLSRGRERCRTELPPLYATDLPGQQAACHFWTEMEAHT
                     AAVRAEVGAERREEAT"
     gene            complement(6024..6962)
                     /locus_tag="VNU01_05925"
     CDS             complement(6024..6962)
                     /locus_tag="VNU01_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH32192.1"
                     /translation="MSSDIRHPPPDAPLGDTAELEREGLSVTPVTQWMLFRRRFVRHK
                     LAMFSLAVLLLVVAAALFAEQIAPYAFDEIDLRNRSRAPTLDGWHILGTDKIGRDYFS
                     RVLFGARTSVRVAFTVAVLSTTIGTVVGAVAGYYRGWVDDLLMRLVDFILAVPFLAVL
                     LVAAAYLGRGSPMRVAVILALLLWTNIARVVRGQYLSLREKEFVEAARASGAGDARII
                     FRHMLPNTLSPIIVNATLVVALAILLESTLSFLGFGIQPPNPALGKLIADGQGSMLTQ
                     WWLVTMPGLVIVAICLSINFIGDGLRDALDPTQPRR"
     gene            complement(6955..7926)
                     /locus_tag="VNU01_05930"
     CDS             complement(6955..7926)
                     /locus_tag="VNU01_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH32193.1"
                     /translation="MLTYVARRLLFSIPVLALASVVVFFGVASVGDPLAELRLKPGVS
                     QITIDNVIERKHLDEPLPQRYGYWVREAVTDRFGETVFGRPIWPDLTRALKNTMQLVL
                     VAEVLSLLIGVPIGILSARRQYSLFDYSATVVSFLGYSIPIFWFALILQVVFTNLYRA
                     TGTRLFYTSGLSSVDPGTGLAFLLDRMQHLALPIIAIAYINVALYSRYMRASMLETLH
                     ADYVRTARAKGVREGTVTRRHAVRNALIPIATLAAINFGATFGGAIVTESIFSLDGMG
                     LFFLRALGAREVYSVMAFLMITSIIVVVANLVADMVYGLLDPRIRYE"
     gene            complement(8032..9708)
                     /locus_tag="VNU01_05935"
     CDS             complement(8032..9708)
                     /locus_tag="VNU01_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HWH32194.1"
                     /translation="MQGFTRSPSRWLLLLVTLAMLATACGGGDGGTEPDGDATETEQA
                     QGEPVDGGTVTFGQDQEPAILMEWFNEGNLQATSIPMKAVLYPLWRITPEFSYEPLLL
                     DGEPEVTEDPFTVTYKLKDEAVWSDGTPITVDDIEFTLDVVLNPDLDIVSRDGYDKID
                     RGQTERIDDKTIKYTFTETYAAYREMFSTQPILPAHALEGQDIATAMNDSVPVASGPF
                     EFESWTKGQNITLVRNENYWGEQKPRLDRVVFRFIEDSNSQVQALRGDEIDMFYPQPQ
                     LELVEQVSELDGVTYEVSAGPVWEHLDFNFAVPPMDQTFVRQAIIQGIDRQALAQELI
                     APMYPEATVLQNVIWMNNQEPYEEHWDRWEYDPEAAIALLEENGCTRGDDEIFTCDGQ
                     RLSFEYVTTAGNELRELQFEIIQSQLQEIGIEARPKLQDAATAFGTTLIAGKEGAWGM
                     FNFAWVGSPDPASGNTIWMCGGGQNYNSYCNEEVTRLIEATNATIDQQERADLYNQAD
                     ELMAADVPLVPLYQKPTFFAWRSALVGMQDNPTQWGPTWNIEEWARTDAA"
     gene            complement(9899..>11309)
                     /locus_tag="VNU01_05940"
     CDS             complement(9899..>11309)
                     /locus_tag="VNU01_05940"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWH32195.1"
                     /translation="SNGIGADAFALVWDGESVHGLNASGRSPALLDAARLRGAAAMPL
                     LGWDSVTVPGAVSAWAALSERFGALPFAALFTDAVRHARDGHPVAPVVAAMWSRAPFT
                     YAGLEDFAEVFLPGGRPPAAGERWASAAHAETLEAIAGTGGEAFYRGELAARTAAAAR
                     AAGAALREEDLAAHQPEWVAPLALEALGARLLELPPNGQGIAALIAAGLVDRRGAALD
                     PDSPDGMHVAIEATKLALADAYAHVADPAAMRVAPEALLDAGYLDERAGRIDLAQAGD
                     PGHGEPRRGGTVCLAAADRRGRAVSFIQSNYMGFGSGVVVPGTGIALHNRGAGFVTTP
                     GHPNVVAGGKRPFHTIIPGMVLGPAGPLLSYGLMGGHMQAQGHLQLLLRILAHGQGPQ
                     AAADAPRWRVDGGRRVAVEPGIPDSVREALAARGHDVRVEDPVPGFGGAQLVWPLEMP
                     ARSWGAAGDWRKDGMPAAR"
BASE COUNT         1430 a         4098 c         4239 g         1542 t
ORIGIN      
        1 gtcgtggacc tcatgcgcca gccggagatc gacggggcgc tggtaggagg cgccagcctg
       61 gacgccgagg acttcgcggt catttgcagg taccaccgcc tgtaggggcg tagcatgagc
      121 cgtgacgctg acccgcgacg atcggggagt tccatgccga agcgccggaa ccacctggac
      181 gggctgctcg gcccgctgga gaccgaggtc atggaggcct gctgggagct cggcgacacc
      241 accgtgcgcg acgtgcacga gaagctcgcc aagcgcctgg acctggccta caccacgatc
      301 atgaccacca tgacgcgcct ggcgggcaag ggcctgctca agcgcgacac cacggggctc
      361 gcccaccgct accgcccggc gctcagccgg gacgagtacg ccagctccac ggtgcacaac
      421 gtcgtcgagt ggctggtgaa ctccttcccc gagccggcga tgagctactt cgcgcaggtc
      481 gtcggcgatg gcgacacggg tgagctcgag gcgctccgcg accgcatcga ccggctgcgc
      541 gaccaggagg cctagtgccc ctcgacggct cgggaggctt cctgctctcc ctgctgacgg
      601 agtcgttcgc ggtccgggcc ctgatcggct cgctggccgc ggcgctgctc gccctgctcg
      661 tggtgcgccg cgacgtggtg cgccaccgca cggcccgccg ggtcacggtg ctcgcccccg
      721 tgctggtggc cgcgggcgcg gccgtcgccg tggcgcgcga gggcggcgcc tacctgccgc
      781 agctgtgggt ggccggcggc cgcggtcccg ccggggagat cctggagctg ctgggggagt
      841 ggcgcgtgtt cgtgcccggc tccggcgtgg acgtcctggt cggcggctac ctggtctgcg
      901 cgggtgtgct cctgggccgg cgcgtggcgg gcgcccggcg gctgcgccgg ctgctcgcgg
      961 gcgccatcga gcccgtcggc tacggccgcc tgatgccggc cctgcaccgg ctcagcgcca
     1021 aggtcggcgt gcgcgcgccg cggctcgtgc tgctggagga ctgcccgggc ggggcgttca
     1081 ccgccggcct gcgccggccg gtcctcgcgc tggacccggt gctggtggac cgcctggacg
     1141 accgcgagct ggagggcctg ctggcgcacg agctggcgca cctgaggcgc cgggacaacc
     1201 acgtggggct ggccgtgggc ctgttccgcg acctggtgtt cttcctgccc cccgtgcacc
     1261 tggcgcagcg ctggctgcgc cgcgagcagg aggagagcgc cgacgagctg gccagcgagc
     1321 acaccggccg cccggtcgcg ctggcctcca gcatcctgaa ggtgtgggac cggcaccgtg
     1381 agcgcccgcc ggcgtggcgc ccgcgcctgg cgtgctccgc gctcggcacg gccgaggcgg
     1441 cgcccgcggc cgcccgcgac gcgctggccc tgcgcgtgga acggctgctg gagggcggcg
     1501 cgcgggcgct gagcccgcgc cggcggcgcc tggagatcgc cctggcgatg gcgctgctcg
     1561 tggccggcgg cctggccgcc tgggtcgtgc cagggtggat ctcccgggac ctgaacgcct
     1621 actcgctgtc gttcatctac ctcaccgcgc cggcggccgg cgaggtcgag gcgccggcgt
     1681 tcgcgacgtt ccgggcgctg accagcgagg cctcggcccg ccccggcctg cggtccgggc
     1741 aggcgatggc cgcccccggc ccatcgggcg aggcggcatc gggcgaaagg gcggtgcgcg
     1801 acccggcgct gtgcccgtgc gtggagtccg ttgcccagct gcgcgcgggc gaggtgttcg
     1861 aggccgaccc gccgtcgccc cgcatgctgt ggcgcgaggc gggccacgac gcctggcagc
     1921 tcagccgcct gcgcgagcgc ggcggcctgc actcccggcc gctgctgacc ctgacggagg
     1981 gcggcccgca gatgggcttt ttcgtggtcg ggcgcccgcc cgcctcctag cggcggggag
     2041 ccgccgggcg catcggctgc taccatgccg gttcgacccg tggcgcgtct gcgccatcga
     2101 acgccggaac gctggaggga ccgtgctcac acccatcctg gtggtgctgc acatcatcgt
     2161 gtcgctgatc ctgatcatgc tgatcctgct caagtccgga cgcggcggcg gcctgtccga
     2221 catgttcggc ggcggcgcga tcacctccat gcagggctcc accgtcgtgg agaagaacct
     2281 cgaccgcctc accgtggtga tcggcgtgct gttcggcgtc acgacgatcg tgctggcgct
     2341 ccggctcgtc tgagccgatg cgccgggccc ggcgcaccac gctgctcctg gtcgccctgg
     2401 ccctggcggc cgcgtgcgac cccggcgccg cgccgcctcc cgccgcgccc agtcccgcgg
     2461 gggcgacctc ggcgcccccg tccgcgacgc tgcgtgtcgc gatcgaccgt ccggacgccc
     2521 tcctgccgca gcgcgccgac gagcaggccg agcagtggct ggccgacgcc gtcttcgaca
     2581 gcctgacgcg ctggggcccc gacctgcggc cggttccggc ggcggcggtg tcgtggagcg
     2641 ccgacgcgtc ccagcgggtg tggacgttcc ggctgcgcga ggacgccgcc tggcacgacg
     2701 ggcggccggt gctggcggcc gacttcgtgc gtggctggcg ggcgctggtg cgcgccgggc
     2761 gcatggcgca cctgctgttc gacgtggaag gcgtggacgg cacggatccg gcgcgggcgg
     2821 agccggccgt ccgggcgctg gacgcgcgca cgctggaggt gcgcctgcgg cggccctccg
     2881 cggacttccc ggccgtggcc gcccaccccg cgctcgcgcc gctgccgccg ggcgcggact
     2941 cggccgagta cgcggcccgg ccggtgggca acgggccgtt cgccgtgccc gaggcgggcg
     3001 cctccagccg cttcgtccgc gtggggcgct tccccggctg gcgcaacggc ccggcgccgg
     3061 ccctggacga gatcgtcttc caggtgatgg accccgagac cgcgttcctg gccttccagc
     3121 agcagcgcgt gcacctgtcg cccgtgccgc cgggggcgct gaccgccctg ctcaccgagt
     3181 ctcccgggtc ggcgtggcgc ggggagcgcg ccgtgctgta cctgctgggc atggaccaca
     3241 cgcgcccgcc cttcgaccgg gtggaggcgc gcagggcgct gagcctggcg gtggaccgtg
     3301 tcgcgctggc cgccgcggtg tccgagggcg ccgccgctcc cgccggcggg ctgctgcccg
     3361 cgtggctgcc ggaggccgtg cccgagcgct gcggctcgtg cgtgcgggac cccggcgcgg
     3421 cgcgcgagct gttcgcccag gccggcgtga cggcgctgcg cctgagcttc aacaccggcg
     3481 ccggccacga gaccgtggcg cggcgcgtgc gcgacgacct cgccgaggcc ggcgtcgcgc
     3541 tggagttcga cgccaggccc ggaccggagt tcctcgcggc cgcccgcgcc ggcgagctca
     3601 ccctgttccg gctcggctgg cagccggacg tgccggtggc cggcgcggtg ctgcgcccgc
     3661 tgttgcacgg cgacgccgtg ccgtccgggc cggagggcgt ccgccacaac ctcgggcgct
     3721 accgggccgc cgacgtcgac gcgctgctgg accaggcgcg ccagacggcg gcgccgctcg
     3781 tgcgcgccgc cacgtaccgc cgtgcggcga ggctggcgct ggagcgcgac caggccgtgg
     3841 tgccgctgtt cgtctcgcgc caccggctcg ccagggcgcc cgaggcggcg ggcctggcgc
     3901 ccgacccgat ggggcaggtg gactgggccg cggtgcggct ggagcgctag gcgcgtgtct
     3961 cggcgtagtg gcaggcggcg gggtggccgt gcccgaagcg gtcggcgagc tccggcacct
     4021 cctgggcgca gcggtcctgg gccttccagc agcgcgtgcg gaacacgcag ccggacggcg
     4081 ggctcacagg gctgggcacg tcgccgctga gcacgatgcg ctcccgccgg ccccgcagcg
     4141 cggggtcggg gatcggcacg gccgacagca gggcctgggt gtagggatgc gtcggccggc
     4201 ggtacacctg cgggcgctcg ccctgctcca tgatccggcc caggtacatg accgccacgc
     4261 ggtcggagat gtggcgcacc acggacaggt cgtgggcgat gaacaggtag gccaggccca
     4321 gctcgtcctg gagctcgcgc agcaggttca cgatctgcgc ctggatcgac acgtccaggg
     4381 cggacaccgg ctcgtccagc accagcacct gcgggtccag cgccagggcg cgcgcgatgc
     4441 cgatgcgctg gcgctggccg ccggagaact cgtgcgggaa gcggttgccg tgctcgggcg
     4501 acaggccgac cagccgcagc agctcggcga cgcggcgttc gccgccgccg gaggtccact
     4561 ccccgtggat gcgcagcggc tcggccacga tgtcgcgcac ggtcatgcgc gggttcagcg
     4621 acgcgtacgg gtcctggaac acgatctgca cctcgcggcg cagctcgcgc aggcgcttgc
     4681 ccctccagtt cgtgacgtcc tcgcccttga acaggatgcg cccggcggtg ggctccagca
     4741 gccggaccac catgcgtccc agcgtggact tgccgcagcc ggactcgccc accagcccca
     4801 gcgtctcgcc ggcgtgcagc gccaggtcca cgccctcgac ggcgtgcacg gttgccacct
     4861 ggcgcttgag cagcccgccg gtgacgggga agtgcttcac caggccctcg acgcgcagca
     4921 cctcctcgcc cgcggcggcg cgccggtgca ggtccgctcc gctcgcggcc acgctcatgt
     4981 cgcctcctcc cggcgctccg cgccgacctc ggcgcgcacc gcggcggtgt gggcttccat
     5041 ctccgtccag aagtggcagg cggcctgctg gccgggcagg tcggtggcgt acagcggcgg
     5101 cagctcggtg cggcagcgct cccgtccgcg cgacaggcgg cagcgcgggt ggaaggggca
     5161 gcccgacggg acgtcgatca ggctcggcgg ctggccgggg atgggcgaca ggcgctcctg
     5221 gtcgcggtcc agccgcggca ggctcgccag cagccccagg gtgtaggggt ggcgcggccg
     5281 ggcgaacagc gtctccgcgt cggccatctc cacgatgcgg ccggcgtaca tgacggcgac
     5341 gcggtcggcg agctccgcca ccacgccgag gtcgtgggtg atcaggatgg tggccgcctc
     5401 ggtctccttc tgcgccgccc gcaggacgtc cagcacctgc gcctggatgg tgacgtccag
     5461 cgcggtggtg ggctcgtcgg ccacgaggat cgccgggcgg ttcgccatcg ccatggcgat
     5521 catggcgcgc tggcgcatgc cgccggagta ctcgtgcggg tactgggcgg cgcgctcccg
     5581 cgcgtccggc acgccgacca gctccagcag ctccaccacg cggcgggcgc gctcgccggc
     5641 ggaaccgccg ggatcgtgga ccgccagcgc ctcggcgatc tgctcgccga tgcgcagcac
     5701 cgggttcagc gacgtcatgg ggtcctggaa caccatcgcg atctcgcggc cgcggatctg
     5761 ccgcagttcg cgcccgcgca ggcccaagag ctcgcggccg cgcaggcggg cgctgcccga
     5821 cgcgatgcgc ccgggcggct gggggacgag cccgagcagc gccatggccg acacgctctt
     5881 gccggacccc gactccccga cgatggccag cgtctcgccc gggtacaggt cgtagtccat
     5941 gccggcgacg gcgtgcacca cgccgtcggg cgtggcgaac tccaccacca ggtcgcggac
     6001 gctgagcacg ggctcgcgca cgttcatcgg cgcggctggg tggggtcgag ggcgtcgcgc
     6061 aggccgtcgc cgatgaagtt gatcgacagg cagatcgcca cgatcaccag gcccggcatg
     6121 gtcaccagcc accactgcgt gagcatggag ccctgcccgt cggcgatcag cttgcccagc
     6181 gccgggttcg gcggctggat gccgaagccc aggaacgaca gcgtcgactc cagcaggatc
     6241 gccagggcca ccaccagcgt cgcgttcacg atgatcgggc tgagcgtgtt gggcagcatg
     6301 tggcggaaga tgatgcgcgc gtccccggcg ccggaggcgc gagccgcctc cacgaactcc
     6361 ttctcgcgca ggctcaggta ctggccgcgc accacgcggg cgatgttggt ccacagcagc
     6421 agcgccagga tcaccgcgac gcgcatcggg gagccgcggc ccaggtacgc ggcggccacc
     6481 agcagcaccg ccaggaacgg cacggcgagg atgaagtcca ccaggcgcat cagcaggtcg
     6541 tcgacccagc cgcggtagta gccggcgacg gcgcccacca cggtgccgat ggtggtggac
     6601 agcaccgcga cggtgaacgc cacgcgcacg gaggtgcgcg cgccgaacag cacgcggctg
     6661 aagtagtcgc ggccgatctt gtcggtcccc aggatgtgcc agccgtccag cgtgggtgcg
     6721 cgcgagcggt tgcgcaggtc gatctcgtcg aatgcgtacg gcgcgatctg ctcggcgaac
     6781 agcgccgcgg ccacgacgag cagcaggacg gcgagcgaga acatcgccag cttgtggcgc
     6841 acgaagcgcc gccggaacag catccactgc gtcaccggcg tgacgctcag gccctcgcgc
     6901 tccagctcgg cggtgtcgcc gagcggcgcg tcgggcggcg ggtggcggat gtcgctactc
     6961 atagcggatc cgcgggtcga gcaggccgta gaccatgtcg gcgacgaggt tggccaccac
     7021 cacgatgatc gacgtgatca tgaggaaggc catgacggag tagacctcgc gcgcccccag
     7081 cgcgcgcagg aagaacaggc ccatgccatc cagggagaag atggactcgg tgacgatggc
     7141 gccgccgaac gtggcgccga agttgatggc cgcgagcgtg gcgatgggga tgagggcgtt
     7201 gcgcaccgcg tgccgccgcg tgacggtgcc ctcgcgcacg cccttggccc gtgcggtgcg
     7261 cacgtagtcg gcgtgcagcg tctccagcat ggacgcccgc atgtagcggc tgtacagcgc
     7321 cacgttgatg taggcgatgg cgatgatcgg cagcgccagg tgctgcatgc ggtccaggag
     7381 gaacgccagg ccggtgcccg ggtccaccga tgacaggccg gacgtgtaga acaggcgcgt
     7441 gccggtggcg cggtacaggt tcgtgaacac cacctgcagg atcagcgcga accagaagat
     7501 cgggatggag tagcccagga agctcaccac ggtggcggag tagtcgaaca gggagtactg
     7561 ccgccgggcc gacaggatcc cgatgggcac gccgatcagc agcgacagca cctcggccac
     7621 cagcaccagc tgcatggtgt tcttgagcgc ccgtgtgagg tcgggccaga tggggcggcc
     7681 gaacacggtc tcgccgaagc ggtccgtgac cgcctcgcgc acccagtacc cgtagcgctg
     7741 gggcagcggc tcgtccaggt gcttgcgctc gatcacgttg tcgatggtga tctggctgac
     7801 gccgggcttc aggcgcagct cggcgagcgg gtcgcccacg gacgcgaccc cgaagaacac
     7861 caccacgctg gccagcgcca gcacggggat gctgaacagc aggcggcggg cgacgtaggt
     7921 cagcacttcg gcgggctcga tgtgggaagg ggcggaacgt gcgccgcctg gcccgggacg
     7981 ccctcgtgct gagggcgccg ggccaggcgg ctgcaagtca cgtcacgatg cctaggcggc
     8041 gtcggtgcgc gcccactcct cgatgttcca ggtggggccc cactgcgtcg ggttgtcctg
     8101 catcccgacc agggcggagc gccacgcgaa gaacgtcggc ttctggtaca gcggcaccag
     8161 cgggacgtcg gcggccatga gctcgtcggc ctggttgtac aggtcggcgc gctcctgctg
     8221 gtcgatcgtg gcgttggtgg cctcgatcag gcgggtgacc tcctcgttgc agtacgagtt
     8281 gtagttctgg ccaccgccgc acatccagat ggtgttgccg ctcgcggggt cggggctgcc
     8341 gacccacgcg aagttgaaca tgccccaggc gccttccttg ccggcgatca gggtggtgcc
     8401 gaacgccgtc gcggcgtcct gcagcttcgg ccgcgcctcg atgccgatct cctgcagctg
     8461 gctctggatg atctcgaact gcagctcgcg cagctcgttg ccggccgtgg tcacgtactc
     8521 gaacgacagg cgctgcccgt cgcaggtgaa gatctcgtcg tcacctcggg tgcagccgtt
     8581 ctcctccagc agcgcgatcg cggcctccgg gtcgtactcc caacggtccc agtgctcctc
     8641 gtagggctcc tggttgttca tccagatgac gttctggagc acggtggcct ccgggtacat
     8701 cggcgcgatc agctcctggg ccagggcctg gcggtcgatg ccctggatga tcgcctggcg
     8761 cacgaaggtc tggtccatgg gcggcaccgc gaagttgaag tccaggtgct cccacaccgg
     8821 gccggcgctc acctcgtagg tcacgccgtc cagctcgctg acctgctcca ccagctccag
     8881 ctgcggctgg gggtagaaca tgtcgatctc gtcgccgcgc agcgcctgca cctgcgagtt
     8941 ggagtcctcg atgaagcgga acaccacccg gtccaggcgc ggcttctgct caccccagta
     9001 gttctcgttg cggaccaggg tgatgttctg ccccttggtc cagctctcga actcgaacgg
     9061 cccggacgcc accggcacgg agtcgttcat cgcggtggcg atgtcctggc cctccagcgc
     9121 gtgggcggga aggatcggct gggtggagaa catctcgcgg taggccgcgt aggtctccgt
     9181 gaacgtgtac ttgatggtct tgtcgtcgat gcgctcggtc tggccacggt cgatcttgtc
     9241 gtagccgtcg cgcgacacga tgtcgaggtc ggggttcagg accacgtcga gcgtgaactc
     9301 gatgtcgtcg acggtgatcg gcgtcccgtc gctccagacc gcctcgtcct tgagcttgta
     9361 ggtcaccgtg aaggggtcct cggtgacctc cggctcgccg tccagcagca gcggctcgta
     9421 ggagaactcg ggcgtgatgc gccacagcgg gtacagcacc gccttcatcg ggatggaggt
     9481 ggcctgcagg ttgccctcgt tgaaccactc catcaggatc gcgggctctt ggtcctgccc
     9541 gaaggtcacg gtcccgccgt cgacgggctc cccctgggcc tgctcggttt cggtggcgtc
     9601 accgtccggc tccgtgccgc cgtccccgcc gccgcacgcg gtggcgagca tggcgagcgt
     9661 gaccagcagc aacagccagc gtgacgggct gcgcgtgaat ccctgcattc ggatcctcct
     9721 gaaaacgggc ttcgcaaggc ccagacccta ccggacgacc gggtggccgc cgcttcaacg
     9781 ttgcgcaaat gtccggcatt cggttccacg ccgactaccg cgatagtgcg ggaccctacc
     9841 aagcatcccc ctgttcgggg aggggcatgc acccgatccg acggccgcgt gacgcggctc
     9901 agcgggccgc gggcatgccg tccttgcgcc agtcgccggc cgcaccccag gagcgcgcag
     9961 gcatctccag gggccacacc agttgcgccc cgccgaaccc gggcaccggg tcctccacgc
    10021 gcacgtcgtg gccgcgcgcc gccagggcct cccgaacgga atccgggatg ccgggctcga
    10081 cggcgacgcg ccggcccccg tcgacccgcc agcgcggcgc gtccgcggcc gcctggggac
    10141 cctggccgtg ggccaggatc cgcagcagca gctgcagatg gccctgggcc tgcatgtgcc
    10201 cgcccatcag gccatagctc agcagcgggc cggcggggcc gagcaccatg ccggggatga
    10261 tcgtgtggaa ggggcgcttg ccgcccgcca ccacgttggg gtggccgggc gtcgtgacga
    10321 agcccgcgcc gcggttgtgc agggcgatgc cggtgcccgg caccaccacg ccggagccga
    10381 agcccatgta gttggactgg atgaaggaga cggcgcggcc gcggcggtcc gcggccgcca
    10441 ggcacaccgt gccgccccgt cgcggctcgc cgtggccggg gtcgcccgcc tgcgcgaggt
    10501 cgatgcggcc ggcgcgctcg tccaggtagc cggcgtccag cagcgcctcg ggcgccacgc
    10561 gcatcgcggc agggtccgcg acgtgggcgt aggcgtcggc cagcgccagc ttggtggcct
    10621 cgatcgcgac gtgcatgccg tccgggctgt ccgggtccag cgcggcgccg cgccggtcca
    10681 ccagcccggc ggcgatcagg gccgcgatgc cctgcccgtt gggcggcagc tccagcaggc
    10741 gggcgcccag ggcctccagc gccagcggcg ccacccattc cggctggtgg gccgccaggt
    10801 cctcctcgcg cagggcggcg ccggccgcgc gtgcggccgc ggcggtgcgg gccgccagct
    10861 cgccgcggta gaaggcctcg ccgcccgtgc ccgcgatcgc ctccagcgtc tcggcgtgcg
    10921 ccgcggacgc ccagcgctcg ccggccgccg gcgggcggcc gcccggcagg aagacctcgg
    10981 cgaagtcctc caggcccgcg taggtgaacg gcgcgcggct ccacatcgcc gccaccacgg
    11041 gcgccacggg atggccgtcg cgcgcgtgcc gcacggcgtc ggtgaacagc gcggcgaagg
    11101 gcagcgcgcc gaagcgctcc gacagggccg cccaggccga gaccgcgccg ggcaccgtga
    11161 cgctgtccca gccgagcagc ggcatggccg cggcgccgcg cagccgggcg gcgtcgagca
    11221 gggcggggga gcgcccggac gcgttgaggc cgtggaccga ctcgccgtcc cacaccagcg
    11281 cgaacgcgtc cgcgccgatg ccgttggac
//
LOCUS       DATMKC010000204         4277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_326655, whole genome shotgun sequence.
ACCESSION   DATMKC010000204 DATMKC010000000
VERSION     DATMKC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326655"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="obgE"
                     /locus_tag="VNU01_05945"
     CDS             complement(<1..1034)
                     /gene="obgE"
                     /locus_tag="VNU01_05945"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWH32196.1"
                     /translation="MFIDEVKVHVKGGRGGSGVTSFARQPFEPRGRPDGGDGGHGGSV
                     VVRATHDVASLVDYHHRPHRAAAGGRHGEGDLRRGADGDDVVLLVPRGTVVKDPDGNV
                     LADLLAEGDEYTAARGGRGGRGNAAFRTSARRAPMFHEYGEPGQERWIVLELKLVADV
                     ALVGFPNAGKSSLIARLSAAKPKIADYPFTTLAPNLGVVRADDADFVVADVPGLIEGA
                     SEGRGLGHQFLRHVERAGALVHVLDCATYEQRDPREDLATVVDELRAYMPDLLDRPAL
                     VFLNKADADPDTAEIVRPDLEADGWEVLVGSAVTGQGVEELRWRMAGLVREARAAHVE
                     QAQDGFAVRAV"
     gene            complement(1103..1636)
                     /locus_tag="VNU01_05950"
     CDS             complement(1103..1636)
                     /locus_tag="VNU01_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32197.1"
                     /translation="MTRIGLTLDELLAATWANGRQPRYLAHLPGLDVLEGETVALVGE
                     GAGAMAEAFAAAVDGALPVDGAIAAQGGTLRIHAQQAARVGVRALAVSDPFPGLSPEA
                     RSLAVADLASFAGLGLTTLVAASDQDAALLFADRVVVLAGGAVRASYPVTAAYPRTPE
                     VVRPVADRLAARLAAVA"
     gene            complement(1808..2059)
                     /gene="rpmA"
                     /locus_tag="VNU01_05955"
     CDS             complement(1808..2059)
                     /gene="rpmA"
                     /locus_tag="VNU01_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWH32198.1"
                     /translation="MAHKKGGGSTNNGRDSNPKYLGVKRYGGETVTTGSIIVRQRGTK
                     IHPGRNVGRGGDDTLFALVDGTVAFRTSGPRKYAEVITG"
     gene            complement(2069..2380)
                     /gene="rplU"
                     /locus_tag="VNU01_05960"
     CDS             complement(2069..2380)
                     /gene="rplU"
                     /locus_tag="VNU01_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWH32199.1"
                     /translation="MYAVVATGGKQYRVKVGDTLDVEKLVPGEDGKVALRPVLLIADD
                     GALTTAKDALAKATVTATVVEQRKGPKLTVFKYKNKTGYRNKNGHRQSLTRIRVETID
                     A"
     gene            complement(2469..>4277)
                     /locus_tag="VNU01_05965"
     CDS             complement(2469..>4277)
                     /locus_tag="VNU01_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWH32200.1"
                     /translation="GASASFLGALAPAAFPVPAARDRERGAGRSGASPGASPGADAKP
                     APKRRPAAAKAEASTADDTGQDEAAGQDEEADAEGGDRPVLKRTRRGRRGGRNRNRSR
                     TARDEAAEGDAPARQEPKAKTPARADAKAPAGDDDDETPTRLQARARRGPTATRGRGR
                     ATGRRPIGGVSDEVRRVIAESPPRRMLVTVGGERTQIAVLEERTLVEHYVTRKQDVSY
                     VGNIYLGRVQNVLPGMEAAFVDIGKGRNGVLYAGEVNYDEADLDGELPRIEEQLKAGQ
                     TVLVQVTKDPMGTKGARLTQHLSIAGRYCVLAPGEGMLGISRKLPDDERDRLRVILKA
                     IRPEGFGLIVRTAAEGATREQLEADVSRLVRIWEQVDERAKKAKPLDTVYEEPDLVIR
                     VIRDVFGPEYTELIVDSEELASQIRDYLGDVSPELVDRVKAHPIEASEGGDAPANLFD
                     AYEVTNQIRRALEKKVWLKSGGYLIVEKTEAMWVIDVNTGKFVGERNLEATVLKNNIE
                     AAEEIARQLRLRDMGGIIVIDFVDMLVPANRDEVLKRFKRELARDKTKSRVMEISKLG
                     LVQMTRKNVSVGLQESFTTKCEVCEGRGAKIVERIV"
BASE COUNT          511 a         1568 c         1558 g          640 t
ORIGIN      
        1 accgcgcgca ctgcgaagcc gtcctgtgcc tgctcgacgt gggcggcgcg cgcctcgcgc
       61 accaggccgg ccatgcgcca gcgcagctct tccacaccct ggccggtcac cgcgctgccg
      121 accaggacct cccagccgtc cgcctccagg tcggggcgca cgatctcggc cgtgtcggga
      181 tccgcgtcgg ccttgttcag gaacaccaag gcgggccggt cgagcaggtc cggcatgtag
      241 gcgcgcagct cgtcgaccac cgtggccagg tcctcgcgcg ggtcgcgctg ctcgtaggtg
      301 gcgcagtcca gcacgtgcac cagcgccccg gcgcgctcga cgtggcggag gaactgatgg
      361 cccagcccgc ggccctcgct ggcgccctcg atcaggccgg gcacgtcggc gaccacgaag
      421 tcggcgtcgt cggcgcgcac gacgccgagg ttgggcgcga gcgtggtgaa cgggtagtcg
      481 gcgatcttcg gcttcgcggc cgacagccgc gcgatcagcg agctcttgcc ggcgttgggg
      541 aagccgacca gcgccacgtc ggcgaccagc ttgagctcca ggacgatcca gcgctcctgg
      601 cccggctcgc cgtactcgtg gaacatcggc gcccgccgcg cggacgtgcg gaacgccgcg
      661 ttgccgcggc cgccgcggcc accgcgcgcg gcggtgtact cgtcgccctc ggcgagcagg
      721 tcggcgagca cgttgccgtc ggggtccttg accacggtgc cgcgcgggac cagcagcacc
      781 acgtcgtcgc cgtcggcgcc gcgacgcagg tcgccctcgc cgtggcggcc gccggccgcg
      841 gcgcggtgcg gccggtggtg gtagtcgacg agactggcga cgtcgtgggt cgcgcgcacg
      901 acgacgctgc cgccgtggcc gccgtcgccg ccgtcggggc ggccgcgggg ctcgaacggc
      961 tggcgcgcga aggacgtgac gccgctgccg ccgcggccgc ccttgacgtg gaccttgacc
     1021 tcgtcgatga acatgaaggc ccagcctagc ggcggccccg atcgcggccg gacgggcgcc
     1081 gtgcgggcgg gaggttggcg cgtcaggcga cggcggcgag gcgggcggcc aggcggtccg
     1141 ccaccggccg caccacctcg ggggtgcggg ggtaggcggc ggtcaccggg taggaggccc
     1201 gcacggcgcc gccggccagc acgaccacgc ggtcggcgaa cagcagcgcc gcgtcctggt
     1261 cggacgccgc gaccagtgtg gtcagaccca gcccggcgaa gctggcgagg tcggcgaccg
     1321 cgagcgacct ggcctcgggg gagaggccgg ggaaggggtc gctcacggcc agcgcccgca
     1381 cgccgacgcg cgccgcctgc tgggcatgga tgcgcagcgt gccgccctgc gccgcgatcg
     1441 cgccgtcgac ggggagcgcg ccgtcgacgg cggccgcgaa cgcctcggcc atcgcgcccg
     1501 cgccctcgcc gaccagcgcc accgtctcac cctccaggac gtccagtccc ggcaggtggg
     1561 cgaggtagcg cggctgccgc ccgttcgccc aggtggcggc gagcaactcg tcgagggtca
     1621 ggccgatgcg cgtcatgcgg gcaacggtag cggcgcggcg agcagcgccc aacgtggaaa
     1681 cagtgggaac ctcggtcggc gacagtgaga acaatgcaaa acgccccggc cgctcggcca
     1741 gggcgttcac tgtgggcgga aaccctcgta tccgagggtt tgtgcccact gtgggcggaa
     1801 gtcggcgtca gccggtgatg acctcggcgt acttgcgcgg cccggaggtc cggaacgcca
     1861 ccgtgccgtc gaccagggcg aacagcgtgt cgtcgccgcc gcggccgacg ttgcggcccg
     1921 ggtggatctt ggtaccgcgc tggcgcacga tgatcgagcc ggtggtgacc gtctcgccgc
     1981 cgtagcgctt cacacccagg tacttcgggt tggagtcgcg gccgttgttg gtcgagccgc
     2041 cgcccttttt gtgagccatg ggattctcct aggcgtcgat cgtctcgacc cggatgcggg
     2101 tgagcgactg ccggtgaccg ttcttgttgc ggtagccggt cttgttcttg tacttgaaca
     2161 ccgtgagctt ggggcccttg cgctgctcca ccacggtggc ggtgacggtg gccttggcca
     2221 gcgcgtcctt ggccgtggtg agcgcgccgt cgtcggcgat cagcagcacg gggcgcagcg
     2281 cgaccttgcc gtcctcaccc gggacgagct tctcgacgtc gagggtgtcc ccgaccttca
     2341 cgcggtactg cttcccgcct gtggcgacaa cggcgtacat cgaaggtgct cctgattttc
     2401 ggacaaagga aaggcgcgcc tgggcgcacc ggtcccgaca gtgtaggacc gggccgccag
     2461 acgggcaact agacgatgcg ctccacgatc ttcgcgccgc ggccctcgca gacctcgcac
     2521 ttggtggtga agctctcctg cagccccacg ctgacgttct tgcgcgtcat ctgcaccagg
     2581 cccagcttgg agatctccat cacgcggctc ttggtcttgt cccgcgccag ctcgcgcttg
     2641 aagcgcttga gcacctcgtc gcggttggcc ggcaccagca tgtccacgaa gtcgatgacg
     2701 atgatgccgc ccatgtcgcg cagccggagc tgccgcgcga tctcctcggc cgcctcgatg
     2761 ttgttcttca gcaccgtggc ctcgaggttg cgctcaccga cgaacttgcc ggtgttcacg
     2821 tcgatgaccc acatcgcctc ggtcttctcg acgatgaggt agccgccgga cttgagccac
     2881 accttcttct ccagcgcgcg gcggatctgg ttggtgacct cgtaggcgtc gaacaggttc
     2941 gccggcgcgt cgccgccctc gctcgcctcg atcgggtgcg ccttgacgcg gtccaccagc
     3001 tccgggctga cgtcgcccag gtagtcgcgg atctgggacg ccagctcctc ggagtccacg
     3061 atcagctcgg tgtactccgg gccgaacacg tcgcggatga cgcggatgac caggtccggc
     3121 tcctcgtaga cggtgtccag cggcttggcc ttcttggcgc gctcgtccac ctgctcccag
     3181 atgcgcacga ggcggctgac gtcggcctcg agctgctcgc gcgtggcgcc ctcggcggcc
     3241 gtgcgcacga tcagtccgaa gccctccggg cgaatcgcct tgaggatcac gcgcaggcgg
     3301 tcgcgctcgt cgtcgggcag cttgcggctg atgccgagca tgccctcgcc gggcgccagc
     3361 acgcagtagc ggccggcgat cgacaggtgc tgggtgagcc gggcgccctt ggtccccatc
     3421 gggtccttgg tgacctgcac caggacggtc tggccggcct tgagctgctc ctcgatgcgc
     3481 ggcagctcgc cgtccaggtc ggcctcgtcg tagttgacct cgccggcgta gagcacgccg
     3541 ttgcgcccct tgccgatgtc gacgaacgcc gcctccatgc ccggcagcac gttctgcacg
     3601 cgacccaggt agatgttgcc gacgtagctc acatcctgct tgcgggtcac gtagtgctcg
     3661 accagcgtgc gctcctccag caccgcgatc tgcgtgcgct cgccgcccac ggtcaccagc
     3721 atccggcgcg ggggcgactc ggcgatcacg cgccgcacct cgtcgctcac cccgccgatc
     3781 gggcgccggc ccgtcgcccg cccgcggccg cgcgtcgccg tggggccgcg gcgggcgcgc
     3841 gcctgcaggc gcgtgggcgt ctcgtcgtcg tcgtcgccgg ccggggcctt ggcgtcggcg
     3901 cgcgccgggg tcttggcctt gggctcctgg cgcgccgggg cgtcgccctc cgcggcctcg
     3961 tcacgcgcgg tgcggctgcg gttgcggttg cggccgccgc gccggccgcg gcgcgtgcgc
     4021 ttgagcaccg gccggtcgcc gccctccgca tcagcctcct cgtcctggcc ggccgcctcg
     4081 tcctggccgg tgtcgtcggc ggtggacgcc tccgccttcg cggcggcggg acgccgcttc
     4141 ggcgccggct tggcgtcggc gccgggcgac gcgccgggcg acgcgccgga gcggcccgcg
     4201 ccccgttcgc ggtcgcgtgc cgccggcacg gggaaggcgg ccggcgccaa ggcgcccaag
     4261 aaggacgccg acgcccc
//
LOCUS       DATMKC010000205        10706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_121513, whole genome shotgun sequence.
ACCESSION   DATMKC010000205 DATMKC010000000
VERSION     DATMKC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121513"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1982
                     /locus_tag="VNU01_05970"
     CDS             180..1982
                     /locus_tag="VNU01_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWH32201.1"
                     /translation="MSRRFAAVFALAAIVLALAPAAYAHEERASQFPAGDGSVPTYRT
                     QEQAADVLVVCKKDGDTAQRIARMTGADKHRNTKLFGQCQFEHIQAAVDAVTKPGTNI
                     YVLPGTYREEPSHDENAACTQGWDGGALEYADIVECGEIINLITVAGDSVDDPDIACD
                     NALCDLQIEGTGATAEDVVVRGGFKPNGDWVKHNGIKADRADGFYLRNMTFELFRENA
                     IYVHETDGYVLDDVVARYNDLYGILTFTSDHGLISDCDTHHNGDSGVYPGSAADVNAD
                     NASPPPLTRWAVEITGCSTHHNALGFSGTAGNSVYFHDNDVWANSAGYVTDSFIPNHP
                     GMPQDHAWLENNRIHGNNTNYYKNVQGDDAPCKRAKPADRGYEDGVVCPAFPVPVGTG
                     VMIAGGNRNLLRANHIYDNWRTGAMLFWVPAALREEFGPVEQIDTSNGNEYRDNIMGR
                     TPEGLVQPNGMDFWWDDQGLGNCWQGNVSATGAVTHNATSPAGLPDCDSGGSVSPVGN
                     AVKSATLVPCATYDREDNPDPDGCDWFDDQQEPAGRQESADASAPALQPTVPVAAENL
                     PAAGGPAALGVLTVGMAALLGAGKLRQRRSPLGR"
     gene            1982..2557
                     /locus_tag="VNU01_05975"
     CDS             1982..2557
                     /locus_tag="VNU01_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32202.1"
                     /translation="MRRAVALATAAIVAVAGAALVAGTGVSGADELHVDGSDRLVVLP
                     RYGPNGSDVVGYRHGETVTVRFPLVNRGLLPLTVEAVEPFPIQLGLLVAEEIRVDGRA
                     LPVTLSPRQRADVTVTARFDNCAYFTERAINPYEHAVVRWRAAGLGHTTQVTYADALL
                     VRSPTIIDCPGRVMDRSAKQRNRDATITGRG"
     gene            complement(2554..3435)
                     /locus_tag="VNU01_05980"
     CDS             complement(2554..3435)
                     /locus_tag="VNU01_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32203.1"
                     /translation="MEDVAVMRLGADGARRVVAILVLAGMLLGVGAGVAELARAQVPE
                     LPLPLPTGEEPSEDPTSPPPRSPDPTPQPSPTVVPEPTWAPSEEFTTSPFQPAPPAPD
                     DGVVPAPGPSAAPAERPPPPRRVVVAGLSPVAAALPGAFAVTLAAVLAVAGLGTGMLA
                     MAKRGLFAARAEDGGIDVDATKRWRLLGGLGLLGAAAIVGVIGWLKISLETQVAVQMV
                     YVASTGFAVLVLAVAGGSLVIAEQLRADERRLREVEDALVNLAGQVQPLVSEPPRIIE
                     RGKTTKAFDPDVTTELR"
     gene            complement(3440..4171)
                     /locus_tag="VNU01_05985"
     CDS             complement(3440..4171)
                     /locus_tag="VNU01_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32204.1"
                     /translation="MSEPLLELRDVSSGYGDLRVLHGVSLTVAPGEVVALMGSNGAGK
                     TTTLKTIVGLLRPMGGSVRYDGQDISRTPPEELVGRGLVLTPEGRGMLRDLTVRQNLE
                     LGGYVIGKDRAELQRRLEEAFDAYPILRERESQLAGTLSGGQQQMLAIVRAMMSRPRL
                     LLIDEASMGLSPIMAQGTFDLIAQLSREQGTAVLLVEQNVEALRLAHRALVLEKGQIV
                     QEARGDQLDELTRRLQDVYLGEVTA"
     gene            complement(4168..6168)
                     /locus_tag="VNU01_05990"
     CDS             complement(4168..6168)
                     /locus_tag="VNU01_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HWH32205.1"
                     /translation="MAVLRRLRAPAARRALLPLGLVAAAVAVPATLGETQVASLTETV
                     AIAIALLSLNVLLGYAGQISLGHAAFVGTGAFLAVQLGGRGLPLPLAVLGAGLGVGAL
                     TALVGLPSLRIRGLAVAIATLVYGIAAQNFLFEQGWFTGADAGLLISAPLGFGAVYRF
                     FLVALGFLAAVLLVDRQLLRTKFGRSLVVVRDAEDRAVAFGVEPGPAKLRAYALSGIL
                     AGIAGALIAYLTGGGSAGDFGVLRSLELLAFAVVGGIGSRGGVLAAAVLLIGVPRLFP
                     PPDARLAPLLGGLLLIGVILFLPDGFGGLLRRLRVRWEALGTQARTALAGVAALAVAL
                     TVARAQDAANDAALAAALLSLAAVVAGVALRGRAPALPSPVTAAPALPDADAAALRDV
                     PRALGLRMPTAALLEVEAVSVRFGGVQALSDVSLQVRQGEIVGLIGANGAGKSTLFNV
                     ASGFVTPTGGSLRYRGRELLTTAPARRTELGIGRTFQHMGLLRPETVQDNVLLAQHWL
                     ARYPSWAGILRLGDTTLTERRLRQRAELALELFGLTGLRHEPLGALPYGTMRMVELAS
                     AVATGADLLLFDEATAGLGPEEAHGLADVWLRLRGELGLTLLVIEHHVPMIARTCDYV
                     YALASGRTLAEGRPADVQSNPDVVAEFLGRSTIDAGGERSGA"
     gene            complement(6170..7075)
                     /locus_tag="VNU01_05995"
     CDS             complement(6170..7075)
                     /locus_tag="VNU01_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWH32206.1"
                     /translation="MLGAVVAALGDGAILALVATGLVLVFKGSRVLNLAQGEIGAFAL
                     LVVYVALGKQSAPGFALLAGATLLGGVLGIGTERLIMRPLVERPPIQSTIASLGLTVV
                     LVNAAALNGTDGVLGFLPQGQNSYPLTMPPTAGRGSVVVAGATLVSGRLVALVATAAV
                     GAALWLFFTRTRFGLAVLAATSDNTVARILGVPVRNVYRFTWGVGGALSGLAAALVAS
                     LGPFQPATMTFTLIGALAGAVIGGLDSVPGAIVGSLLVGVVQGLIKYHFPSVGGVENV
                     AVLALVLVTLMVRPRGLLGGAGATA"
     gene            complement(7076..8542)
                     /locus_tag="VNU01_06000"
     CDS             complement(7076..8542)
                     /locus_tag="VNU01_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH32207.1"
                     /translation="MTVSHPAPRRTPRLALLLVLCVAVAACGPRFDRDLVAGSGVDFA
                     GGPESGEIVDGPNFPGQDGIVTDPGLGSDEGAGPGADPGAAPPDGGTTTGPSTTGPGT
                     AAGPGSAPRTPAQGGGGSAQVAPGPQPGVTDTTLKIGYLVPLTGAAPIPTTWDDGANL
                     YWDVRNDAGGINGRKVELIIEDTESSTTTAVTKARKLVTQDKVFTIVTLDRLEVQEKV
                     AQYLEGVGMPHVMVQSPASPPATWKNTFTISVDHVVQGRGIARFWASDLGAGAGRKKV
                     GFVREQTNALKPGTDAFEAEAVRLGLDVVARETINPQQVDFSQTVLRLQQSGAEIVWL
                     YMAPTPAATIIGQSGTAGYRPTWFANSISWGFELMHGVTGGFMEGAYAFSPWVSLNNP
                     RTKAYRDAWSSRNGGTADDIGLVGWGVGGVLDAALSRPGRPLGWDSFRSSMRTFKAAT
                     DVWVPVDFGGGGSIGTNQVAVFKAQGGAWHTVGDFRGL"
     gene            8651..9664
                     /locus_tag="VNU01_06005"
     CDS             8651..9664
                     /locus_tag="VNU01_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32208.1"
                     /translation="MVEGRTTARFPQVGDGRAHYESFYVKAADPRGGKAVWLRHTVHQ
                     RPGEAATGSLWLTVFDEDRGAPSALKVTEPAAALSAPDGAYIQVADAVLAPGRAVGTM
                     RADGIEASWDLAFTPGDETLWHLPASFLYSAPLPKTKSLSPHPHALWTGTVRLGEVEL
                     SLEAWPGMVGHNWGSEHAERWIWLHGDPFREGAATAGDAWIDMTFGRIKVGRWTTPWV
                     GNGMLTLDGRRHRLGGIPAIRATSVTETPTTLEFSVPGKGIEVRGRVHEPPGAAAAWA
                     YADPDGSWHNAVNCSRATLELQVRKAGDPVRHLVCDGAAAYELGMRETNHGIPLQPWR
                     DGR"
     gene            9674..10465
                     /locus_tag="VNU01_06010"
     CDS             9674..10465
                     /locus_tag="VNU01_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWH32209.1"
                     /translation="MTGYDTLRVARDDRGVATVTMDRAEVRNAFDPALIAELTAVAAE
                     LAADGAVRVVVLTGAGSVFSAGADLNWMKAMQAYTYEENVADSRRLDAMFRALWELPQ
                     PVVGRINGHALGGGTGLTAVCDIAIASDAATFGFTEVLLGIAPAVISPYVLRKTSLSF
                     ARSVFVTGERFSAERAREAGLVHEVVAAGALDDAVEAAVRRCLAAGPTAAAAAKRLPE
                     IALLPLDDAAAQTVEIIAGLRVAEEGQEGMAAFFDKRKPRWVSEG"
     gene            complement(10452..>10706)
                     /locus_tag="VNU01_06015"
     CDS             complement(10452..>10706)
                     /locus_tag="VNU01_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWH32210.1"
                     /translation="RIDLGEAALLLDASDGAPRVEPAHADGTGQSGAVTVEADPHALL
                     AVASGAETLRAARRRGAVRMSGDRDGAQRLERLFAAISPR"
BASE COUNT         1511 a         4095 c         3849 g         1251 t
ORIGIN      
        1 cggcacggcg ggccgcgccg agaagccgcc cgtgcggcta ggctcgcccc agcggttcgc
       61 cggcgtccac gccgggaatg cgcccaaggc aggagcacgg caggagtcgg tgcccgccac
      121 ggcgaatccc cacaggagac gccgtccggg cggaccggac agcacgggag gtgggccgca
      181 tgtcgcgccg ttttgccgca gtcttcgcgc tggccgcgat cgttctcgca ctcgcaccgg
      241 ccgcgtacgc gcacgaggag cgcgcgtcgc agttccccgc cggtgatggc agcgtgccga
      301 cctaccgcac gcaggagcag gccgccgacg tgctggtggt gtgcaagaag gacggcgaca
      361 cggcgcagcg catcgcgcgc atgacgggag ccgacaagca ccgcaacacc aagctgttcg
      421 gccagtgcca gttcgagcac atccaggcgg ccgtcgacgc ggtcaccaag ccgggtacca
      481 acatctacgt gctgcccggc acctatcgcg aggagccgtc gcacgacgag aacgcggcgt
      541 gcacgcaggg ctgggacggt ggcgcgctgg agtacgccga catcgtggag tgcggcgaga
      601 tcatcaacct gatcacggtg gcgggcgact cggtcgacga ccctgacatc gcctgcgaca
      661 acgcgctgtg cgacctgcag atcgaaggca ccggcgcgac cgccgaggac gtggtggtgc
      721 gcggcggctt caagcccaac ggcgactggg tcaagcacaa cggcatcaag gctgaccggg
      781 ccgacggctt ctacctgcgc aacatgacct tcgagctgtt ccgtgagaac gccatctacg
      841 tgcacgagac cgacggctac gtgctcgacg acgtcgtcgc ccgctacaac gacctctacg
      901 gcatcctcac gttcacctcc gaccacggcc tcatctccga ctgcgacacc caccacaacg
      961 gcgactccgg tgtctacccg ggctccgcgg ccgacgtgaa cgccgacaac gcctcgccgc
     1021 cgcccctgac ccggtgggcc gtggagatca ccggctgctc cacgcaccac aacgcgctgg
     1081 gcttctccgg caccgccgga aactccgtgt acttccacga caacgacgtg tgggccaaca
     1141 gcgccggcta cgtgaccgac agcttcatcc ccaaccaccc cggcatgccg caggaccacg
     1201 cctggctgga gaacaaccgc atccacggca acaacacgaa ctactacaag aacgtgcagg
     1261 gcgacgacgc gccgtgcaag cgcgcgaagc cggccgaccg cggttacgag gacggcgtgg
     1321 tgtgcccggc gttcccggtg ccggtgggca cgggcgtgat gatcgcgggc gggaaccgca
     1381 acctcctgcg cgccaaccac atctacgaca actggcgcac cggcgcgatg ctgttctggg
     1441 tgccggccgc gctgcgcgag gagttcgggc cggtcgagca gatcgacacg tccaacggca
     1501 acgagtaccg cgacaacatc atgggccgta caccggaggg cctggtgcag cccaacggca
     1561 tggacttctg gtgggacgac cagggtctgg gcaactgctg gcagggcaac gtctcggcca
     1621 ccggcgcggt gacgcacaac gccaccagcc cggccgggct gcccgactgc gacagcggcg
     1681 gctccgtctc gcccgtcggc aacgcggtga agagcgccac gcttgtgccc tgcgccacct
     1741 acgaccgcga ggacaacccg gaccccgacg gctgcgactg gttcgacgac cagcaggagc
     1801 cggccggtcg ccaggagtcc gctgacgcct cggcgccggc gctgcagccc accgtgccgg
     1861 tggcggccga gaacctgccg gccgccggcg gccccgccgc actgggcgtc ctgacggtgg
     1921 gcatggccgc gctgctgggt gccggcaagc tgcgacagcg ccgctcaccg ctggggcgct
     1981 gatgcgccgg gcggtcgcgc tggccacggc ggccatcgtc gccgtggccg gtgcggcgct
     2041 ggtggccggc accggcgtca gcggggccga cgagctgcac gtcgacggct cggaccggct
     2101 tgtggtcctg ccgcggtacg ggcccaacgg ctccgatgtc gtgggctacc gccacggcga
     2161 gacggtcacc gtgcgcttcc cgctggtgaa ccgcggcctg ctgccgctga ccgtcgaggc
     2221 cgtggagccg ttccccatcc agctcggcct gctcgtggcc gaggagatcc gcgtggacgg
     2281 ccgcgcgctg ccggtgaccc tgtcaccgcg tcagcgcgcc gacgtgaccg tcaccgcgcg
     2341 cttcgacaac tgcgcctact tcaccgagcg ggcgatcaac ccgtacgagc acgccgtggt
     2401 gcgctggcgt gccgccggtc tggggcacac cacgcaggtg acctacgccg acgcgctgct
     2461 ggtgcgctcg ccgacgatca tcgactgccc cgggcgggtg atggaccgca gcgccaagca
     2521 gcgcaaccgc gacgccacca tcaccggccg cggctagcgc agctcggtgg tgacgtccgg
     2581 gtcgaacgcc ttggtggtct tgccgcgctc gatgatccgc gggggctcgg acaccagcgg
     2641 ctgcacctgg ccggccaggt tcaccagcgc gtcctccacc tcccgcagcc gtcgctcgtc
     2701 ggcgcgcagc tgctcggcga tgaccaggct gccgcccgcc accgccagca ccagcaccgc
     2761 gaatcccgtg ctggccacgt agaccatctg cacggccacc tgcgtctcca gcgagatctt
     2821 cagccagccg atcacgccca cgatcgcggc cgcgcccagt aggcccagtc cgccgagcag
     2881 ccgccagcgc ttggtcgcgt ccacgtcgat gcctccgtcc tctgcgcggg ccgcgaacag
     2941 cccgcgcttt gccatcgcca gcatgccggt tcccaggccc gccacggcga gcaccgccgc
     3001 gagcgtcact gcgaacgcgc ccggcagcgc cgccgccacc gggctcagtc ccgccaccac
     3061 cacgcgccgc ggcggtggtg gccgctccgc cggcgccgca ctcgggcccg gcgcgggcac
     3121 cacgccgtcg tccggcgccg ggggcgccgg ctggaacggc gaggtggtga actcctccga
     3181 gggcgcccac gtcggctccg gcaccacggt cggtgacggc tgcggcgtcg gatcggggct
     3241 gcgcggcggc ggggaggtcg ggtcctcgct gggctcctca ccggtcggca gcggcagcgg
     3301 gagttcgggg acctgcgccc gcgcgagttc ggccacgccg gcgccgacgc ccagcagcat
     3361 cccggccagc accaggatcg ccaccacgcg gcgcgcgccg tcggctccca ggcgcatcac
     3421 ggcgacgtcc tccacgccgt catgccgtga cctcccccag gtagacgtcc tggaggcggc
     3481 gcgtgagctc gtcgagctgg tcgccgcgcg cctcctgcac gatctgcccc ttctccagca
     3541 ccagcgcgcg gtgcgccagg cgcagcgcct ccacgttctg ctccaccagc agcacggccg
     3601 tgccctgctc gcggctgagc tgcgcgatca ggtcgaacgt gccctgcgcc atgatcggcg
     3661 acagccccat cgacgcctcg tcgatcagca gcagccgcgg ccgcgacatc atcgcgcgca
     3721 cgatggccag catctgctgc tgcccgccgg acagcgtccc cgcgagctgc gactcgcgct
     3781 cgcgcaggat cgggtaggcg tcgaacgcct cctccagccg gcgctgcagc tccgcgcggt
     3841 ccttgccgat gacgtagccg cccagctcca ggttctggcg cacggtgagg tcgcgcagca
     3901 tgccgcggcc ctcgggcgtc agcaccagcc cgcgcccgac cagctcctcc ggcggcgtgc
     3961 gcgagatgtc ctgcccgtcg tagcgcacgg acccgcccat gggccgcagc aggccgacga
     4021 tggtcttgag cgtcgtggtc ttgcccgcgc cgttggagcc catgagcgcg accacctcgc
     4081 cgggcgccac ggtcagcgac acgccgtgca gcacgcgcag gtcgccgtac cccgacgaca
     4141 cgtcgcgcag ctccagcagg ggctcgctca tgcgccgctc cgctcgccgc ccgcgtcgat
     4201 ggtgctgcgg cccaggaact ccgcgaccac gtccgggttg gactgcacgt ccgcggggcg
     4261 gccctcggcg agcgtgcggc cggaggccag cgcgtacacg tagtcgcagg tcctggcgat
     4321 catcggcaca tggtgctcga tcaccagcag cgtcaggccc agctcgccgc gcaggcgcag
     4381 ccacacgtcg gccagcccgt gcgcctcctc gggacccagg ccggcggtgg cctcgtcgaa
     4441 cagcagcagg tcggcgcccg tggccacggc cgaggccagc tccaccatgc gcatcgtccc
     4501 gtacggcagg gcgcccagcg gctcgtggcg caaccccgtg aggccgaaca gctccagcgc
     4561 cagctcggcg cgctggcgca gccgccgctc ggtcagcgtg gtgtcgccca gtcgcaggat
     4621 gcccgcccag ctcgggtagc gggccagcca gtgctgggcc agcagcacgt tgtcctggac
     4681 ggtctccggc cgcagcaggc ccatgtgctg gaacgtgcgc ccgatgccca gctccgtgcg
     4741 ccgcgcgggc gccgtggtca gaagctcccg gccgcggtag cgcagcgagc cgccggtcgg
     4801 cgtgacgaag ccggacgcca cgttgaacag cgtcgacttg cccgcgccgt tcgcgccgat
     4861 gagccccacg atctcgccct ggcgcacctg cagcgacacg tccgacagcg cttgcacgcc
     4921 gccgaagcgc acgctgaccg cctccacctc cagcagcgcc gccgtcggca tgcgcagccc
     4981 cagcgcgcgc gggacgtcgc gcagcgccgc cgcgtccgcg tccggcagcg ccggcgccgc
     5041 ggtgaccggc gagggcaggg ccggcgcccg gccgcgcagc gcgaccccgg ccaccaccgc
     5101 cgccagcgac agcagcgccg cggccagcgc cgcgtcgttg gccgcgtcct gtgcgcgcgc
     5161 cacggtgagc gcgacggcca gggccgccac gccggccagc gccgtgcgcg cctgcgtgcc
     5221 cagcgcctcc cagcgcacgc gcagccgccg caacagcccc ccgaagccgt ccggcaggaa
     5281 caggatcacg ccgatcagca gcaggccgcc cagcaggggc gccagccgcg cgtccggcgg
     5341 cgggaacagc cgcggcacgc cgatgagcag caccgccgcc gccagcacgc cgccgcgcga
     5401 gccgatgccg ccgaccaccg cgaacgccag gagctcaagg gagcgcagca cgccgaagtc
     5461 cccggccgag ccgccgcccg tcaggtaggc gatcagcgcg ccggcgatgc cggccaggat
     5521 ccccgacagc gcgtacgcgc gcagcttcgc cggccccggc tccacgccga acgccacggc
     5581 gcggtcctcg gcgtcgcgca cgacgaccag cgagcggccg aacttggtgc gcagcagctg
     5641 gcggtccacc agcagcaccg cggccaggaa gccgagcgcg accaggaaga agcggtacac
     5701 cgccccgaag cccagcggcg cgctgatcag caggcccgcg tcggcgccgg tgaaccagcc
     5761 ctgctcgaac aggaagttct gcgcggcgat gccgtacacc agcgtggcga tggcgaccgc
     5821 cagcccgcgg atgcgcaggg acggcaggcc gaccagcgcc gtgagcgcac cgacgcccag
     5881 gccggcgccc aggaccgcca gcggcagcgg caggccgcgg ccgccgagct gcacggccag
     5941 gaacgccccg gtgcccacga acgccgcgtg gcccagcgag atctgcccgg cgtagcccag
     6001 cagcacgttc agcgacagca gcgcgatggc gatggccacg gtctcggtga ggctcgcgac
     6061 ctgcgtctcg ccgagcgtgg cgggcacggc gaccgccgcg gcgaccaggc caagcggcag
     6121 cagcgcccgg cgcgccgcgg gcgcgcgcag gcggcgcagc actgccatgt caggcggtcg
     6181 caccggcacc tcccagaagg ccccgcgggc gcaccatcag cgtgaccagc accagggcca
     6241 ggacggcgac gttctcgacg ccgccgacgc tggggaagtg gtacttgatc agcccctgca
     6301 ccacgcccac cagcaggctg ccgacgatcg cgcccggcac ggagtccagg ccgccgatca
     6361 ccgcgcccgc cagcgcgccg atcagcgtga acgtcatggt ggccggctgg aacgggccca
     6421 ggctggcgac cagggcggcg gccaggccgc tcagcgcgcc gcccacaccc caggtgaagc
     6481 ggtacacgtt gcgcaccggc acgcccagga tgcgcgccac ggtgttgtcg ctggtggcgg
     6541 ccaggaccgc caggccgaag cgcgtgcgcg tgaagaacag ccacagggcg gcgccgaccg
     6601 cggccgtggc gaccagggcc accaggcgcc cggacaccag cgtcgcgccc gccaccacca
     6661 cggagccgcg ccccgccgtg ggcggcatgg tcagcgggta gctgttctgg ccctgcggca
     6721 ggaagccgag cacgccgtcg gtgccgttga gcgcggcggc gttgaccagc accacggtca
     6781 gccccagcga ggcgatggtg ctctggatcg gcgggcgctc caccagcggg cgcatgatca
     6841 gccgctcggt gccgatgccg agcacgccgc ccagcagcgt ggcgccggcc agcagcgcga
     6901 accccggtgc ggattgcttg cccagcgcca cgtagaccac cagcagcgcg aacgcgccga
     6961 tctcgccctg cgccaggttc agcacccgcg agcccttgaa caccagcacc aggccggtgg
     7021 cgaccagggc caggatggcg ccgtcgccga gcgccgcgac gaccgccccg agcatctaca
     7081 ggccccggaa gtcgccgacg gtgtgccagg cgccgccctg cgccttgaac acggcgacct
     7141 ggttggtgcc gatggagccg ccgccgccga agtcgaccgg cacccacacg tccgtggcgg
     7201 ccttgaacgt ccgcatcgag gagcggaacg agtcccagcc cagggggcgg ccagggcggc
     7261 tgagcgcggc gtcgaggacg ccgccgacgc cccagccgac caggccgatg tcgtcggccg
     7321 tgccgccgtt gcgggaggac caggcgtccc tgtaggcctt ggtgcgcggg ttgttgagcg
     7381 acacccacgg cgagaacgcg taggcgccct ccatgaagcc gccggtcacg ccgtgcatca
     7441 gctcgaagcc ccagctgatc gagttggcga accaggtggg ccggtagccg gcggtgccgg
     7501 actggccgat gatcgtcgcg gccggcgtcg gcgccatgta cagccacacg atctccgcgc
     7561 cggactgctg cagccgcagg accgtctggc tgaagtcgac ctgctgcggg ttgatcgtct
     7621 cgcgcgccac cacgtccagg cccagcctga cggcctcggc ctcgaacgcg tccgtgccgg
     7681 gcttcagggc gttggtctgc tcgcgcacga agccgacctt cttcctgccg gcgccggcgc
     7741 ccaggtcgct cgcccagaag cgcgcgatgc cgcggccctg caccacgtgg tccacggaga
     7801 tcgtgaacgt gttcttccac gtggccggcg ggctggcggg cgactgcacc atgacgtggg
     7861 gcatgcccac gccctccagg tactgggcga ccttctcctg cacctccagg cggtccagcg
     7921 tcacgatggt gaacaccttg tcctgcgtca ccagcttgcg cgccttggtc accgccgtgg
     7981 tggtggacga ctcggtgtcc tcgatgatca gctcgacctt gcggccgttg atgccgccgg
     8041 cgtcgttgcg gacgtcccag tacaggttcg cgccgtcgtc ccaggtggtg gggatcggcg
     8101 cggcgcccgt cagcgggacc aggtagccga tcttcagcgt ggtgtccgtg acgcccggct
     8161 gcggtcccgg agcgacctgc gccgagccgc cgccaccctg cgcgggcgtg cgcggcgcgc
     8221 tgccggggcc cgccgccgtg ccgggaccgg tggttgacgg gcccgtcgtc gtgccgccgt
     8281 ccggcggcgc ggcgccgggg tccgcgcccg gcccggcgcc ctcgtcgctg ccaaggcccg
     8341 ggtcggtgac gatgccgtcc tgccccggga agttcggtcc gtcgacgatc tcgccgctct
     8401 ccggcccgcc ggcgaagtcc acgccggagc cggcgacgag gtcgcggtcg aagcgcggcc
     8461 cgcacgcggc gacggccaca cacagcacga gcagcagcgc cagccggggg gtgcggcgcg
     8521 gagcgggatg ggagacggtc acggtgcgtc ctcgcggagt ccgggctgcc gaagcggcgg
     8581 agccgcgaag ggcccgcctg tcgcctcgca gggtactagg ctgcggccgt ccacgagcga
     8641 gggaggcggc atggtcgagg ggcggacgac ggcacggttc ccgcaggtcg gggacgggcg
     8701 cgcgcactac gagagcttct acgtcaaggc ggccgacccg cgcggcggca aggcggtgtg
     8761 gctgcgccac accgtgcacc agcgccccgg cgaggcggcc accggctcgc tgtggctgac
     8821 cgtgttcgac gaggaccgcg gcgcgccgtc cgccctgaag gtgaccgagc cggccgcggc
     8881 gctgtccgcg ccggacggcg cctacatcca ggtcgccgac gcggtgctcg cgcccggccg
     8941 cgccgtgggc acgatgcgcg ccgacggcat cgaggcctcc tgggacctgg cgttcacgcc
     9001 gggcgacgag acgctgtggc acctgcccgc ctcgttcctg tactccgcgc cgctgcccaa
     9061 gaccaagtcg ctgtcgccgc acccgcacgc gctgtggacg ggcacggtgc gcctgggcga
     9121 ggtggagctg tcgctggagg cgtggccggg catggtcggg cacaactggg gcagcgagca
     9181 cgccgagcgc tggatctggc tgcacggcga cccgttccgc gagggcgcgg cgaccgccgg
     9241 ggacgcctgg atcgacatga cgttcgggcg catcaaggtg ggacggtgga ccacgccgtg
     9301 ggtcggcaac gggatgctga ccctggacgg ccggcgccac cgcctcggcg gcatcccggc
     9361 gatccgcgcc acgtcagtga ccgagacgcc gaccacgctg gagttcagcg tgccgggcaa
     9421 gggcatcgag gtgcgcggcc gcgtgcacga gccgccgggc gccgcggcgg cgtgggcgta
     9481 cgccgacccg gacgggagct ggcacaacgc cgtgaactgt tcgcgcgcca cgctggagct
     9541 ccaggtgcgc aaggccggcg acccggtgcg ccacctggtg tgcgacggcg cggccgccta
     9601 cgagctgggc atgcgcgaga ccaaccacgg catcccgctg cagccgtggc gggacggccg
     9661 ctagcctgcc gccatgaccg gctacgacac gctccgcgtg gcgcgcgacg accgcggcgt
     9721 cgccaccgtc accatggacc gcgccgaggt gcgcaacgcc ttcgaccccg cgctgatcgc
     9781 cgagctgacc gccgtggccg ccgagctggc cgccgacggc gcggtccggg tggtggtgct
     9841 gaccggcgcg ggaagcgtct tcagcgccgg cgcggacctc aactggatga aggccatgca
     9901 ggcctacacc tacgaggaga acgtggccga ctcccgccgc ctcgacgcga tgttccgcgc
     9961 gctgtgggag ctgccccagc cggtggtcgg gcgcatcaac ggccacgcgc tcggcggcgg
    10021 caccggcctg accgccgtct gcgacatcgc gatcgccagc gacgcggcga cgttcggctt
    10081 caccgaggtc ctgctcggca tcgcgcccgc cgtcatctcg ccgtacgtgc tgcgcaagac
    10141 gtcgctgtcg ttcgcgcgct cggtgttcgt gacgggtgag cgcttctcgg ccgagcgcgc
    10201 ccgcgaggcg ggcctggtgc acgaggtggt ggcggccggc gcgctggacg acgcggtcga
    10261 ggcggcggtg cgccgctgcc tggccgcggg ccccacggcc gcggcggcgg ccaagcggct
    10321 gcccgagatc gcgctgctgc cgctggacga cgcggccgcg cagaccgtgg agatcatcgc
    10381 cggcctgcgc gtggccgagg agggccagga gggcatggcc gccttcttcg acaagcgcaa
    10441 gccgcgctgg gtcagcgagg gctgatcgcg gcgaacaggc gctccagccg ctgggcgccg
    10501 tccctgtcgc cgctcatccg cacggcgccg cgccggcgcg cggcgcgcag cgtctccgcg
    10561 ccggacgcca cggcgagcag cgcgtgcggg tcggcctcga cggtcacggc gccggactgt
    10621 ccggtgccgt ccgcgtgcgc gggctcgacg cgcggcgcgc cgtcgctggc gtccagcagc
    10681 agtgccgctt cgcccaggtc gatgcg
//
LOCUS       DATMKC010000206         7013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_315143, whole genome shotgun sequence.
ACCESSION   DATMKC010000206 DATMKC010000000
VERSION     DATMKC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315143"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VNU01_06020"
     CDS             complement(<1..490)
                     /locus_tag="VNU01_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWH32211.1"
                     /translation="MPKRELPLHLTRNIGIMAHIDAGKTTTTERILYYTGRNYKIGEV
                     HEGAATMDWMVQEQERGITITSAATTAQWKGHVINIIDTPGHVDFTVEVERSLRILDG
                     AVAVFDAVSGVEPQSETVWRQANKYGVPRICFVNKMDRLGADFFAALDSIHERLDVTT
                     AVI"
     gene            complement(530..1000)
                     /gene="rpsG"
                     /locus_tag="VNU01_06025"
     CDS             complement(530..1000)
                     /gene="rpsG"
                     /locus_tag="VNU01_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWH32212.1"
                     /translation="MPRKGPAPRRKIVADPKHNSTLVTQLINRVMLDGKRSVAERIVY
                     DAFDLIQERTGADPVATFKKAVDNVKPQLEVKSRRVGGATYQVPIEVRQGRGTTLALR
                     WLTEYARARREHTMAERIAGELMDAGNNAGAAVKRREDTHKMAEANKAFAHYRW"
     gene            complement(1005..1376)
                     /gene="rpsL"
                     /locus_tag="VNU01_06030"
     CDS             complement(1005..1376)
                     /gene="rpsL"
                     /locus_tag="VNU01_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWH32213.1"
                     /translation="MPTINQLVRTARQAKTEKAKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTSGIEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYKVIRAAL
                     DASGVRNRKQARSKYGAKKEG"
     gene            complement(1806..2429)
                     /locus_tag="VNU01_06035"
     CDS             complement(1806..2429)
                     /locus_tag="VNU01_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH32214.1"
                     /translation="MTMTTAVVRILIVDDHPTIRSGLKTILTADSRMEVVGEAGSALA
                     GVAALDKAKPDIVLLDLGLPDVRGVGAVRTLLEAGAQKILVLSGAGSAELVRECLQAG
                     AQGFLLKTAPEAALCGAIHRIVGGERVVDDSLIGALFEAPPAGLTPRELEVLRAVADG
                     CTNRQVASRLGVSEDTIKTHLSRAMEKLGAADRTHAIAILLRRGLLD"
     gene            complement(2461..3354)
                     /locus_tag="VNU01_06040"
     CDS             complement(2461..3354)
                     /locus_tag="VNU01_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32215.1"
                     /translation="MKDARGIADAWSRVVAHLDIQIPAERLEAAAAALGGDPAAAWAE
                     ARRQLVAHGAPAHVVRRALFALFGVVTDRDPAVAEQAAPALALLAGAPSLATATRSRT
                     PDLLERVHDGPLQDAIALQLKVRQDPALGELHRAIAGLVSSLRSVLSEGVTRQDVALE
                     AQLAAAVRRCPWAHVTVECTVPEGLNDEIADLVLRVVQESLANLRHADARNAHVQVRA
                     GAEWLRVKVEDDGAGFDARVALTPRPGHLGLPWLSDAIASCGGQLMVRSAHGLGTRVM
                     ADLPLSVRQDAQIPMAHSLTA"
     gene            complement(3821..7013)
                     /locus_tag="VNU01_06045"
                     /pseudo
     CDS             complement(3821..7013)
                     /locus_tag="VNU01_06045"
                     /inference="COORDINATES: protein motif:HMM:NF011498.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          929 a         2432 c         2494 g         1158 t
ORIGIN      
        1 ggatgacggc ggtggtgacg tcgaggcgct cgtggatcga gtcgagggcg gcgaagaagt
       61 cggcccccag gcggtccatc ttgttgacga agcagatgcg ggggacgccg tacttgttcg
      121 cctggcgcca caccgtctcc gactgcggct ccacaccgga cacggcgtcg aagaccgcga
      181 ccgcgccgtc caggatgcgc agcgagcgct ccacctcgac cgtgaagtcc acgtggccgg
      241 gcgtgtcgat gatgttgatc acgtggccct tccactgcgc ggtcgtggca gcggaggtga
      301 tggtgatgcc gcgctcctgc tcctggacca tccagtccat ggtcgcggcg ccctcgtgga
      361 cctcaccgat cttgtagttc cggcccgtgt agtacaggat ccgctcggtg gtcgtggtct
      421 tgcccgcgtc gatgtgcgcc atgatcccga tgttccgggt caggtgcagc gggagctcac
      481 gcttcggcat ggtgtgactc agtccttgtg gtgacgggta ccgccgggat taccagcggt
      541 agtgggcgaa ggccttgttg gcctcggcca tcttgtgggt gtcctcgcgg cgcttgaccg
      601 cggcgccggc gttgttgccg gcgtccatga gctcgccggc gatgcgctcg gccatggtgt
      661 gctcacggcg cgcgcgggcg tactcggtga gccagcgcag ggcgagcgtg gtaccgcgtc
      721 cctggcgcac ctcgatcggc acctggtagg tcgcgccacc gacacggcgg gacttgacct
      781 ccagctgcgg cttgacgttg tcgacggcct tcttgaaggt ggccaccggg tcggcgccgg
      841 tgcgctcctg aatgaggtcg aacgcgtcgt agacgatgcg ctcggccacc gagcgcttgc
      901 cgtccagcat gacgcggttg atgagctgcg tcaccagcgt ggagttgtgc ttcgggtccg
      961 cgacgatctt gcggcgcggg gccgggccct tgcgtggcat gggtctagcc ctccttcttg
     1021 gcgccgtact tggagcgcgc ctgcttgcgg ttgcggacgc cgctggcgtc cagcgccgcg
     1081 cggatgacct tgtagcgcac acccggcagg tccttcacgc ggccgccgcg cacgagcacg
     1141 atggagtgct cctgcaggtt gtgaccctcg ccggggatgt acgcggtgac ctcgatgccc
     1201 gaggtgaggc gcacgcggca gaccttgcgc agcgcggagt tcggcttctt cggcgtggtc
     1261 gtgtacacgc gcgtgcacac gccgcggcgc tgcggcgagc ccttgagggc aggcgtcttc
     1321 gccttctccg tcttcgcctg gcgcgcggtg cggaccagct ggttgatcgt gggcatacgt
     1381 cgtcctgttg gcgttggtgc cgttccccga gccacgggtc cgggcgtttc tcaccttggt
     1441 ggagctgctg ccgggtccgt cccgcgtgcg ggcggcagca tcaagccgtc gctcgtccgc
     1501 ggatcggttg gagatcgcgc gctggtccgt tgtggtaagg cccatcttgc ggaccgcacg
     1561 cgctgcctcc cggaagcgca cgacgaccag cgcgcggctt cgccccgggc cccgcccgga
     1621 gctggctgtc gcgatggtcg actcgactcc tcccgcaaaa gccgggcgaa gtcaggcgcc
     1681 tacggtacgc gtccgcaccc cgggcgtcaa cttggccgcg ggccggcagg gggtacgagg
     1741 ccccacaaag ttggaagatc aggcccacat acgggcctga tcttccaact cggggagagg
     1801 gggggtcagt cgagcaggcc gcgacgcagc aggatcgcga tcgcgtgcgt gcggtcggcg
     1861 gcgccgagct tctccatggc gcgtgacagg tgggtcttga tggtgtcctc ggagacgccg
     1921 agccggctgg cgacctggcg gttggtgcag ccgtcggcca ccgcgcgcag cacctccagc
     1981 tcacgcgggg tgagcccggc gggcggcgcc tcgaacagcg cgccgatcag cgagtcgtcg
     2041 accacgcgct cgccacccac gatgcggtgt atcgcgccgc acagggcggc ctccggcgcg
     2101 gtcttcagca ggaagccctg cgcgcccgcc tgcaggcact cgcgcaccag ctcggccgag
     2161 ccggccccgg acagcaccag gatcttctgg gcgccggcct ccagcagcgt gcgcaccgca
     2221 cccacgccac ggacgtcggg caggcccaag tcgagcaaca cgatgtcggg cttggccttg
     2281 tccagcgccg cgacgccggc cagcgccgag ccggcctcgc ccacgacctc catgcggctg
     2341 tcggccgtga ggatggtctt gagcccggac cggatcgtgg ggtggtcgtc cacgatgagg
     2401 atccggacaa cggcggtcgt catggtcatc ggtgttgctc cttggtcgcg cgtctgcccg
     2461 ctacgcggtg agactgtgag ccatcgggat ctgtgcgtcc tgccggaccg acaggggaag
     2521 gtcggccatc acgcgcgtgc ccaggccgtg cgcggagcgc accatgagct ggccgccgca
     2581 ggaggcgatg gcgtcgctga gccacggcag gccgaggtgg ccgggccgcg gcgtgagcgc
     2641 gacgcgggcg tcgaagccgg cgccgtcgtc ctcgaccttg acccgcagcc actccgcgcc
     2701 ggcgcgcacc tgcacgtggg cgttgcgggc gtcggcgtgg cgcaggttgg ccagcgactc
     2761 ctgcacgacg cgcagcacca ggtcggcgat ctcgtcgttg aggccttccg ggacggtgca
     2821 ctccacggtg acgtgcgccc aggggcagcg gcggaccgcg gccgcgagct gcgcctccaa
     2881 cgcgacgtcc tgtcgtgtga cgccttccga cagcacgctg cgcaggctgc tcaccaggcc
     2941 ggcgatcgcc cggtgcagct caccgagcgc ggggtcctgg cgaaccttga gctgcagcgc
     3001 gatcgcgtct tggagcggtc cgtcgtggac gcgctccagc aggtccgggg tgcgcgaccg
     3061 cgtggccgtg gcgagcgagg gggccccggc gaggagggca agcgccgggg ccgcctgctc
     3121 ggccactgcg ggatcacggt ccgtcacgac gccgaagagg gcgaacagcg cgcgtctgac
     3181 cacgtgcgcc ggagcgccat gggcgacgag ctgccggcgg gcctcggccc acgcggccgc
     3241 cgggtcgcca cccagcgctg ccgcagcggc ttccagacgc tccgcgggga tctggatgtc
     3301 caggtgcgca accacccggc tccatgcgtc cgcgatgcct cgagcatcct tcaccgctgc
     3361 actctcctgg tcgtccgtga ccccagttgc tgctacggag agtgaatcgg caggtgacta
     3421 gtccgagttg agcagcccac acgaaaatcc atcacccgat cggggtgggc acgagtgaac
     3481 ccccgtcccc caccgcccca cagcccgggt gacccccgca atccaggacc tagcgactgg
     3541 acggatggtg ccgccggcgg aggaggcacg gcagcgggaa cgggcgaggc cccgcttccc
     3601 gcgccgctca gcgcaaaggc ctatgggctc ggaactagta ggtgccgcct tcccccatcg
     3661 tccagccctc gtccgtctcg tactcgtagg agccgtcgcc gaactgctcc agcaggtcgg
     3721 cgggcaccag ctgctccggc acctccgtgg gcagcgggcg cacgccgcgg tacttgcgca
     3781 tgcccgtgcc ggccgggatc agcttgccga tgatcacgtt ctccttcagg cccatgaggg
     3841 tgtcgctctt gccctcgatg gcggcctcgg tgagcacgcg cgtggtctcc tggaacgacg
     3901 ccgcggacag ccacgactcg gtggccagcg acgccttggt gatccccatg aggatctggc
     3961 ggccgttggc cggctcgccg gtctcgtcga ccacgcggcg gttctcctcg gtgaagcgga
     4021 tccggtcgac cagctcgccg ggcaggaacg tggtgtcgcc cggctcgatg atggtcacgc
     4081 ggcgcagcat ctgccgcacg atcagctcga tgtgcttgtc gtggatcgac acgccctgtg
     4141 agcggtagac ctcctgcacc tcgcggacca gcagcttctg ggtctcgcgg gcaccgagca
     4201 cctccagcat ctcgtgcggg tcgatggagc cctcggtgag ctggtcgccc acgcgcacct
     4261 ggctgccgtc ctcgacgagc aggcgggcgc gacgcgacac gatcaccgac caggtgtcct
     4321 cgccgccggc gtcgacggag atctggaggc ccttctcggt ctcctccagc gacacccggc
     4381 cggtcaggtg cgcgatctcg gccttgccct tggggctgcg ggcctcgaac agctccacga
     4441 cacgcggcag accgtgcgtg atgtccgcgc cggccacacc accggtgtgg aacgtgcgca
     4501 tcgtcagctg ggtaccgggc tcaccgatcg actgggcggc gatgatgccg accgcctcgc
     4561 cgatctgcac cgaccggccc tcggccagcg cacggccgta gcacaggccg cagacgccct
     4621 gcttggcctc gcagttcagc accgtgcgga ccttgacctc gcggtccccg ggggacggct
     4681 cgttctccag cttctcaccg gtctccgggt cgatgccgtc gcggagcacc gcacgcagca
     4741 ggcggatctc cgcgtcggtg atctccgtgc cggccttcag caggtgcttg cgggtgcccg
     4801 gccagtacac ggtctcggcc aggacacggc cgtacagcga ctggctgtcg cgctcgccca
     4861 ccggcacggt catcccgcgc tcggtgccgc agtcgtcctc gcggatgatc aggtcctggg
     4921 cgatgtccac caggcggcgg gtcaggtagc cggagtccgc cgtgcgcagc gccgtgtcgg
     4981 ccaggccctt gcgggcgccg tgcgtggaga tgaagtactc caggaccgac aggccctcgc
     5041 ggaagttgga cttgatcggc cgctcgatga tctcaccgcg cgggttggcc accaggccgc
     5101 gcatgccggc gatctggcgg atctgcgtca tgttcccgcg ggcaccggag ttggccatca
     5161 tgtagatcgg gttgagccgg gagaagttgc gctccatcgc cgacgccacg cggttggtgg
     5221 cctcggtcca gatctcgatc agttcctgct tgcgctcgtc gtcggtgatg acgcccttgc
     5281 ggaactgctt ctcgacctcc tgcgcctgcg cgtcgtgctc ggcgaggatc tccgccttgg
     5341 ccggcggcgc ctccacgtcg gcgatcgaca cggtgacgcc cgccttggtg gcgtagcgga
     5401 agccgatgtc cttcatgcgg tccaggacct cggcggtctc gtggcgcgtg aagttgtccg
     5461 cgcagcggtt gaccaggtcg gccagctcct tcttgccgac caggctgttc acgtacggca
     5521 gcgcggcggg caggacctcg ttgaacagca cgcggcccgg cgtggtgagc acgcggaccc
     5581 cgcgcgccga cgtgaccacg ccgtcgacga cctcgtcgtc ctcgtccggc tcgccgaaca
     5641 cgtcggcgac gttcaccgca cggtccttga gcttgaccag gacccactgc tgcaggccca
     5701 cgtccttgcc gtccagcgcc atgacgacct cgccggcgcc ggagaacgac gggatcttca
     5761 ccttgccggc cttgacgtcg gcgatgaagg actcgtcggg ggccgtgccg ggggcgtcgc
     5821 ggccctcggc gaaggtgagg tagtacgtgc ccaggaccat gtcctgcgtc ggggtggcga
     5881 tgggacggcc gtgggccggc gacaggatgt tgttggagga cagcatgagg atgcgggcct
     5941 cggcctgcgc ctccgcggac agcggcaggt gcaccgccat ctggtcgccg tcgaaatcgg
     6001 cgttgaacgc cgcgcacacc agcgggtgga tctggatggc cttgccctcg accagacggg
     6061 gctcgaaggc ctggatgccc aggcggtgca gcgtcggggc gcggttcagc agcaccgggt
     6121 ggtcgcggat gacctcctcc agcacgtccc agacctgcgg ccgcgcgcgc tccaccatgc
     6181 gcttggcgct cttgatgttc tgcgcgtagt tgaggtccac cagccgcttc atcacgaacg
     6241 gcttgaacag ctccagcgcc atctgcttgg gcaggccgca ctggtgcagc ttcagctccg
     6301 ggcccaccac gatgaccgag cggcccgagt agtccacgcg cttgcccagc aggttctggc
     6361 ggaagcggcc ctgcttgccc ttgagcatgt cgctcaggga cttcagcggc cggttgccgg
     6421 ggcccgtgac cgggcggccg cggcggccgt tgtcgaacag cgcgtcgacg gcctcctgga
     6481 gcatgcgctt ctcgttgttc acgatgatct cgggcgcgcc caggtccagc agccgcttca
     6541 ggcggttgtt gcggttgatc acgcggcggt acaggtcgtt gaggtccgag gtcgcgaagc
     6601 ggccgccgtc cagctgcacc atcgggcgca ggtccggcgg gatcaccggg atggccttga
     6661 gcaccatcgc ggcggggtcg ttgccggtct ggcggaacgc ctcgaccacc ttgagccgct
     6721 tgacggcgcg cgtggccttc tgcgagcggg ccttgcgggc gccgctcttg atcttctccg
     6781 cctcgtcgct gaggatcgtg cgcagctcct cgctctcggc ctcgaagtcg atctgctgga
     6841 gcaggtcgcg gatcgcctcg gcgcccatgc cgccggtgaa gtactcgccg aagcggtcgc
     6901 gcagctcgcg gtagagcagc tcgtcggcga ccagctgctt gggctcgagc ttgcggaagg
     6961 tgtcgagcac ctcctcgagc ttgtccagct cgcgctcggc gcggttgcgg atc
//
LOCUS       DATMKC010000207        11590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_349906, whole genome shotgun sequence.
ACCESSION   DATMKC010000207 DATMKC010000000
VERSION     DATMKC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349906"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1159)
                     /locus_tag="VNU01_06050"
     CDS             complement(83..1159)
                     /locus_tag="VNU01_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWH32216.1"
                     /translation="MSTLAILGATGYTGRLCATEAVRRGLDVVLAARNPDKLATLAQA
                     LGGPRTAVVDVADGVALRALAEQADVLLTTVGPYLRHGRPALLAALDGGAHYVDITGE
                     VPFQAWVREHDQRAREAGVALCPAFGYDGVPGDLLAGIAARALDAPVEEARAAYLVRH
                     GRASGGTLRSIVGIARSSGAVWQDGVLVTEPVAARSWDVPFPQPLGVRGAVSAPLPDV
                     LTLADSTGASSAQAYLVTAGANALAKAGPPLERATRALLASPLGVLVDRVAERLPEGP
                     APADRQRMKAAVLAEVRGGGRTAAAWCRLRDAYGTTAVITIDVAERLLGGGAPRAGAL
                     TPTELLGEDADAFLRQIGADWAMC"
     gene            complement(1196..2134)
                     /locus_tag="VNU01_06055"
     CDS             complement(1196..2134)
                     /locus_tag="VNU01_06055"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32217.1"
                     /translation="MNGWDEMMRQAGRQYGLIERTQAHGLGMSADALAHRARKEGWPR
                     PQRGVFALPGSADTPERRILAAVLAVQGDAWATRLTAAYVWGLHDRLRVPLTLLVPHQ
                     HRAAVLERATVMRTRNLSPEDVTVRRALPVVTAARMIADLCGVLPIEELRGLAIAARQ
                     MGLLRPEELWALWERFQGSPRHRKLRALATMLSGDVERVDSMLEHRVRALLRRYGLPL
                     PYPEPFPVRAGGRVIACIDIAWPAAMVGVECDGFRYHSKAEDLEKDTRRQNQLVTLGW
                     QLVRVTWRQMEREPELIASAVHRLLVGTGAVSERSL"
     gene            complement(2260..4203)
                     /locus_tag="VNU01_06060"
     CDS             complement(2260..4203)
                     /locus_tag="VNU01_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWH32218.1"
                     /translation="MRVRFSRWSGTQEPFGADVTAEELLDELSDDLIDGVPAEDALRR
                     LLQRGMSGMRGRVAGLDELRRRVEQARRRELERLGLEGPLRQMEERLGEIVERERLTA
                     RFSADTERGQARLDELDALPADLAARVDALRGSEWMDPAARADFDALLEELRRQVAEA
                     TFGRLAGALGSMSPEDLERSSAMLGELNALADRHARGEDVAADFAEFKARYADLIPGD
                     PATIDELLEELARRMAAMSRLLASLDPEQRAQLAQLAEQLLGDLDLSFQAEQLSRTLE
                     SMFPELGWDQAVPGAQPAGELEGSLSATAEWMEHLQSYDELGEALRQDYAGARLEDVD
                     EEALCRALGEDAARDLRALREIERLLEEAGALRRRQGRLELTPKGMRQLGERALARIY
                     ERALSGEPGGHRTPLTGGEGEPTGSTRPLRFGDPFRVDVSRTIFNAVGQGGVPVRVRA
                     EHFELAEAERRVRTSTVLLLDMSFSMPLRGNWGAAKRLALALSSLASSKFPEDRFHVV
                     GFSDYARRMAPEDLLVTGWERVYGTNMQHAFMIARRLLAQRPGDRKQVIMVTDGEPTA
                     HLEGDVAAFAWPPEPDTLRKTMAEALRLARSNTTLNVFLLDHDPGAAQFVERMVRRCG
                     GRIFHPDLDDLGDMVVREFLAPR"
     gene            complement(4200..5609)
                     /locus_tag="VNU01_06065"
     CDS             complement(4200..5609)
                     /locus_tag="VNU01_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH32219.1"
                     /translation="MSTRPRTIAELRAAGYPDRTVKQEVAANAAARLRAGLPVVDGLV
                     GFADTVLPALETALLAGHDLILLGERGQAKSRVLRGLAGLLDEEIPVIAGSEVNDHPY
                     HPFSAAARALVAEHGDATPVEWIGRDRRYAEKLATPDISVSDLIGDVDPMKVAEGRYL
                     SDELTVHFGLIPRHNRGLVAINELPDLAERIQVALLNVMEERDVQVRGYSLRLPLDLL
                     VVATANPEDYTNRGRIITPLKDRFGAEVRTHYPRTIDEEVDVMVQEARMPGGAGLPPV
                     QVPAFMAEVLAAFTRALRAAPEVNQRSGVSVRFSIGNYETLAASALRRAVRTGEDHAV
                     ARPSDLAQVVSACLGKVEFESYEEGREAAILERLLRRALLEVFRRRAAGVDLDALLAK
                     FADGRQVETGDLVPAGELLGQLDEIPGLGRLLGALGVESESPALAASALEFALEGLHL
                     ARKLDRADVGPGAFRYGPA"
     gene            5796..6197
                     /locus_tag="VNU01_06070"
     CDS             5796..6197
                     /locus_tag="VNU01_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32220.1"
                     /translation="MSNPSPLRTASGSSRRPPEGALLRCILDSDRWAESLPPVPSDRL
                     VTLSFTSASAQVEHAEACEMLGYRVVGAPPDAAPPVSVADLLVTQDIIEAHPTWWRAL
                     ATHADRVYSLAHGPAVALLGDVLEPHLAARA"
     gene            complement(6208..6645)
                     /locus_tag="VNU01_06075"
     CDS             complement(6208..6645)
                     /locus_tag="VNU01_06075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32221.1"
                     /translation="MLLRLELQLPRDVRYVGVARNVAESLFRELGCPEDVSADIQLAL
                     TEACANAVRHAVDTEEYSVVLRIDQDCCEIEVVDLGPGFGTRDPRTDRAVGPEVESGR
                     GLLLMRALVDDLEFRALEDGTSVRLRKFWHGLALSPGQKVAAR"
     gene            complement(6810..8228)
                     /locus_tag="VNU01_06080"
     CDS             complement(6810..8228)
                     /locus_tag="VNU01_06080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWH32222.1"
                     /translation="MTGLDAAFLYLETPTNHMHVGGVLVLDPSTTPSGSLTREDMARF
                     MEPRLHLAAPFRRRLAWVPFNLDHPVWIEDPDFDLDLHVRRAALPSPGGMAELAEFAG
                     DVMGRPIDRNHPLWELWVVEGLEGGRVAVITKTHHAAIDGVSGAELAAAILQLTPDQE
                     APDAPRPWRAEREPSDLRLLVQAGLRQAATPLSVARLGRQAFDSATALFKVARTRKEE
                     PPPAPFTAPSTPFNGSIGPRRAVALSTMPLEDLKRVKNALGGTVNDVVLACVAGSLRR
                     FLLRRDEAPERPLVAMVPISVRAEEQRGQMGNQVSAMLAALPVEEPDTLQRLRRISAS
                     TAGAKEQHGALGANTLQQLAELAPGGVASLAARLYTRTRAADRHRPIWNLVVSNVPGP
                     RFPLYCAGARVEAFYPLGPVHEMVGLNVTLFSYEQDVFVGVNADRGLVPDVDDVCEGM
                     GEALQELVELAASHGGRSGKRR"
     gene            8349..9188
                     /locus_tag="VNU01_06085"
     CDS             8349..9188
                     /locus_tag="VNU01_06085"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWH32223.1"
                     /translation="MPDALVTADWVAEHLDDPRVVIAEVDEDAAAYHAGHIPGALGFD
                     WRADFQDPLKRAFLDADGFAALMDSRGITRDHHVVLYGGNNNWFAAYAYWYFKIYGHP
                     AVSLMDGGRKLWELQGRPMTDEPVKREATSGYQAGEPDASIRAKRDQVLAEFVGAPDG
                     KGLIDVRSPQEFAGELIAPPHLPNEGSQVPGHIPGAQNIPWAKAVDPDTGQFLDAGAL
                     KALYTGVGITPDKEVVAYCRIGERSAHSWFVLHEILGYPNVRNYDGSWTEYGSLVDVP
                     VER"
     gene            complement(9381..10481)
                     /locus_tag="VNU01_06090"
     CDS             complement(9381..10481)
                     /locus_tag="VNU01_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWH32224.1"
                     /translation="MIPDRILMGPGPSNVAPDVLAALSQPLLGHLDPEFLPLLDELND
                     RLRQVFRTASPVTFPVSGTGSAGLECVVVNLVEPGDRVVVGVNGVFGGRLAEVARRAG
                     AEVHAVEADWGRIVDPEAFLAACREHRPALAVLVHAETSTGVWQPVAEVAAALRDSET
                     LFAIDCVTSLGGVEVDIDGWGVDAAYSGTQKCLSVPPGLAPVTFGPRATERIRSRRTP
                     CRSWYLDVTGILEYVSAEGGRKYHHTAPISMLYALHAGLGVVLEEGLDARIARHERVG
                     RMLQEGLEALGLPLLAQEGHRLPQLTTVGLPDGVDEAAWRRRLLDEHGIEVGGGLGAL
                     AGRAWRIGLMGHTATEANVRKLLAVLGEWLDG"
     gene            10617..11369
                     /locus_tag="VNU01_06095"
     CDS             10617..11369
                     /locus_tag="VNU01_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32225.1"
                     /translation="MTQVMPGCEAWSAAGGEVGVLVLHGFTGNPVSMRPVAEAMAAAG
                     HAVEMPRLPGHGTTWQECNRTTWRDWAREAVAAYETLRARTGAQVAVGLSMGGTMALH
                     LASTRGDDLAGVVAINPSLKSSDPRLKALPVLKHLIPGLKGIGNDIAKPGGDEKPYPK
                     VPLKALASFLQLQGLVRESLSAVTIPTLVFTSRNDHVVEPENGALVIDGIASADSEQV
                     WLERSYHVATLDYDADEIVQRTLAFIKRVTAN"
BASE COUNT         1569 a         4473 c         4079 g         1469 t
ORIGIN      
        1 accaggaagt cgaacgcccc ttgcggctgt tgctgcgcgg cggccggcgc cggcagtcgc
       61 ccgtgccctt gccggccgcc gctcagcaca tggcccagtc ggcaccgatc tgacgcagga
      121 aagcgtcggc gtcctcgccc agcagctccg tgggcgtgag cgcgccggcg cgcggcgcgc
      181 cgccgcccag caggcgctcg gccacgtcga tcgtgatgac ggcggtggtg ccgtaggcgt
      241 cgcgcaggcg gcaccacgcc gccgcggtcc gcccgccgcc gcgcacctcg gccagcaccg
      301 cggccttcat gcgctggcgg tccgcgggcg cggggccctc gggcaggcgc tccgccacgc
      361 ggtccaccag cacgcccagc ggcgaggcca gcagcgcgcg cgtcgcgcgc tccagtggag
      421 ggccggcctt ggcgagcgcg ttcgcgccgg ccgtgaccag gtacgcctgc gcggaggacg
      481 cgccggtgga gtcggccagc gtcagcacgt ccggcagcgg cgcgctgacc gccccgcgca
      541 cgcccagggg ctgcgggaac ggcacgtccc aggagcgggc cgcgaccggc tcggtgacca
      601 ggacgccgtc ctgccacacc gcaccggagg agcgcgcgat gccgacgatg gagcgcagcg
      661 tgccgccact ggcgcgcccg tggcgcacca ggtaggcggc gcgcgcctcc tcgaccgggg
      721 cgtccagcgc gcgggcggcg atgccggcca gcaggtcgcc gggcacgccg tcgtagccga
      781 acgccgggca cagcgcgacc ccggcctccc tggcgcgctg gtcgtgctcg cgcacccacg
      841 cctggaacgg cacctcgccg gtgatgtcga cgtagtgggc gccgccgtcg agcgcggcga
      901 gcagcgccgg gcggccgtgg cgcaggtacg gcccgaccgt ggtcagcagc acgtcggcct
      961 gctcggccag ggcgcgcagg gccacgccgt cggcgacgtc gacgaccgcc gtgcgcgggc
     1021 cgccgagcgc ctgcgccagc gtcgcgagct tgtccgggtt gcgggcggcg agcaccacgt
     1081 caaggccgcg gcgcaccgcc tcggtggcgc acagccggcc cgtgtagccg gtcgcgccca
     1141 gaatcgccag tgtgctcatg gccgcgcagc ctagctcccc tcgttgggtg cggtttcaga
     1201 gcgatcgctc tgaaaccgca cccgtaccga cgagaagtct gtggacggcc gacgcgatca
     1261 gctcgggttc ccgctccatc tgccgccacg tcacacggac gagctgccag ccgagtgtca
     1321 cgagctggtt ctggcggcgc gtgtccttct ccaggtcctc ggccttcgag tgataacgga
     1381 agccgtcgca ctcgacaccc accatggccg cgggccacgc gatgtcgatg caggcgatga
     1441 cgcgtccgcc cgctcgcacg ggaaagggct ccgggtacgg caggggcaag ccgtaccggc
     1501 gcagcagtgc acgcacccga tgttcgagca tgctgtcgac gcgctccacg tcgccgctca
     1561 gcatcgtcgc cagcgcccgc agcttgcgat gacgcggcga accctgaaag cgctcccaca
     1621 acgcccacag ctcctccggc cggagcagtc ccatctgccg cgcggcgatc gccaagccgc
     1681 gcaactcctc gatcgggagc accccgcaga ggtccgcgat catgcgtgcc gccgtgacca
     1741 caggtagggc gcggcggacc gtcacatcct caggcgagag gttgcgcgtc cgcatcacgg
     1801 tggcgcgctc cagcacggcc gcccgatgct gatgcggcac cagcagggtg agcggcacgc
     1861 gaagacgatc gtgcaggccc cacacgtacg ccgccgtcaa acgcgtcgcc caggcgtcac
     1921 cctgcacggc cagcaccgcc gcgagaatcc ggcgttccgg cgtgtcggcg gagccgggca
     1981 gtgcgaacac accgcgctgc ggacgcggcc acccctcctt gcgcgcccgg tgcgccagcg
     2041 cgtccgcgct catgcccagg ccgtgcgcct gcgtccgctc gatgagcccg tactgccggc
     2101 cggcctgccg catcatctcg tcccacccgt tcatgagcga gagagtctgc atctccgatc
     2161 caggcgcggc agcccgtcat ccacaggctt gcggtcagtg ccgtgcgggt gcggtttcag
     2221 agcgatcgct ctgaaaccgc acccaactcg aaggggggcc tagcgggggg ccaggaactc
     2281 ccgcacgacc atgtcgccca ggtcgtccag gtcggggtgg aagatgcgcc cgccgcagcg
     2341 gcgcaccatg cgctccacga actgggcggc gccggggtcg tggtccagca ggaacacgtt
     2401 cagcgtggtg ttcgagcgcg ccagccgcag cgcctccgcc atcgtcttgc gcagcgtgtc
     2461 cggctccggc ggccaggcga aggccgcgac atcgccctcc aggtgcgcgg tcggctcgcc
     2521 gtcggtcacc atgatcacct gcttgcggtc ccccgggcgc tgggccagca ggcgccgggc
     2581 gatcatgaac gcgtgctgca tgttcgtgcc gtacacgcgc tcccagccgg tcaccagcag
     2641 atcctccggc gccatgcgcc gcgcgtagtc gctgaagccg accacgtgga agcggtcctc
     2701 ggggaacttc gacgacgcca gcgacgacag cgccagcgcc aggcgctttg ccgcgcccca
     2761 gttgccgcgc agcggcatcg agaagctcat gtcgagcagc agcaccgtgg acgtgcgcac
     2821 acgccgctcc gcctcggcca gctcgaagtg ctcggcgcgg acgcgcaccg gcacgccgcc
     2881 ctggcccacc gcgttgaaga tcgtgcgcga cacgtcgacg cggaacgggt cgccgaagcg
     2941 cagcgggcgg gtgctgccgg tcggctcgcc ctcgccgccg gtcagcggcg tgcggtggcc
     3001 ccccggctcg cccgacagcg cgcgctcgta gatgcgcgcg agcgcgcgct cgccgagctg
     3061 gcgcatgccc ttgggcgtga gctccaggcg tccctggcgc cgccgcaggg cgccggcctc
     3121 ctccagcagc cgctcgatct cgcgcagcgc gcgcaggtca cgggcggcgt cctcccccag
     3181 cgcgcggcac agcgcctcct cgtcgacgtc ctccagccgg gcgcccgcgt agtcctgccg
     3241 cagcgcctcg ccgagctcgt cgtaggactg caggtgctcc atccactcgg cggtcgccga
     3301 caggctgccc tccagctccc cggccggctg cgcgccgggc accgcctggt cccagccgag
     3361 ctcggggaac atcgactcca gcgtgcgcga cagctgctcg gcctggaagg acaggtccag
     3421 atcgcccagc agctgctcgg cgagctgcgc gagctgcgcg cgctgctccg ggtcgaggct
     3481 ggccagcagc cggctcatcg cggccatgcg ccgcgccagc tcctccagca gctcgtcgat
     3541 ggtcgccggg tcgcccggga tcaggtcggc gtagcgggcc ttgaactcgg cgaagtcagc
     3601 cgcgacgtcc tcgccgcgcg cgtggcggtc cgccagcgcg ttgagctcgc ccagcatggc
     3661 gctggagcgc tccaggtcct ccggcgacat ggatcccagc gctcccgcca ggcggccgaa
     3721 cgtcgcctcg gcgacctgcc gccgcagctc ctccagcagc gcgtcgaagt ccgcgcgcgc
     3781 ggcagggtcc atccactccg acccgcgcag cgcgtccacg cgcgcggcca ggtccgccgg
     3841 cagcgcgtcg agctcgtcca ggcgcgcctg gccgcgctcg gtgtcggcgc tgaaccgcgc
     3901 ggtcagccgc tcacgctcga cgatctcgcc caggcgctcc tccatctggc gcagcgggcc
     3961 ctccaggccc aggcgctcca gctcccgccg gcgcgcctgc tcgacgcggc ggcgcagctc
     4021 gtccaggccg gccacgcggc cgcgcatgcc gctcatcccc cgctgcagca gccggcgcag
     4081 cgcgtcctcg gcgggcaccc cgtcgatgag gtcgtcggac agctcgtcga gcagctcctc
     4141 ggcggtgacg tccgcgccga agggctcctg cgtgccgctc cagcggctga agcggaccct
     4201 catgcggggc cgtagcggaa cgcgccgggc cccacatcgg cgcggtcgag cttgcgggcc
     4261 aggtgcaggc cctccagggc gaactccagc gccgaggccg ccagcgccgg ggactccgac
     4321 tcgacgccca gcgcgccgag caggcggccc agccccggga tctcgtcgag ctggccgagc
     4381 agctcgccgg ccgggaccag gtcgccggtc tccacctggc ggccgtcggc gaacttcgcc
     4441 agcagcgcgt ccaggtccac cccggcggca cggcggcgga acacctccag cagcgcgcgg
     4501 cgcagcagcc gctccaggat cgccgcctcg cgaccctcct cgtaggactc gaactccacc
     4561 ttgcccaggc atgccgagac cacctgcgcc agatcgctcg gccgcgccac ggcatggtcc
     4621 tcgccggtgc gcacggcgcg gcgcagcgcg ctggcggcca gcgtctcgta gttgccgatg
     4681 gagaagcgca ccgacacccc ggagcgctgg ttgacctctg gcgccgcgcg cagcgcccgg
     4741 gtgaacgcgg ccagcacctc cgccatgaac gccggcacct gtacaggcgg aaggccggcc
     4801 ccgcccggca tgcgggcctc ctgcaccatg acgtccactt cctcgtcgat ggtgcgcggg
     4861 tagtgcgtgc ggacctcggc gccgaagcgg tccttgagcg gggtgatgat gcgcccgcgg
     4921 ttggtgtagt cctccgggtt ggccgtggcc accacgagca ggtccagcgg caggcgcagc
     4981 gagtagccgc gcacctggac gtcgcgctcc tccatcacgt tgagcagcgc cacctggatg
     5041 cgctcggcca ggtccggcag ctcgttgatg gccaccaggc cccggttgtg gcgcgggatc
     5101 agcccgaagt gcacggtgag ctcgtcggac aggtagcggc cctcggcgac cttcatcggg
     5161 tcaacgtcgc cgatcaggtc gctcacggag atgtccggcg tggccagctt ctccgcgtag
     5221 cggcggtccc gcccgatcca ctcgaccggc gtggcgtcgc cgtgctcggc gaccagcgcg
     5281 cgggccgccg cggagaaggg atggtagggg tggtcgttca cctcgctgcc ggcgatgacg
     5341 gggatctcct cgtccagcag gcccgccagg ccgcgcagca cgcggctctt ggcctgcccg
     5401 cgctcgccca gcaggatcag gtcgtggccg gccagcagcg cggtctccag cgccggcagc
     5461 acggtgtcgg cgaagcccac caggccatcg accaccggca ggccggcgcg caggcgggcg
     5521 gcggcgttgg ccgccacctc ctgcttgacg gtgcggtcgg ggtagcccgc cgcccgcagc
     5581 tcggcgatgg tccgtggtcg cgtcgacacc tgggctccta accgggactc cgtaccgggg
     5641 ctgtttcgtc gtgccgcccc ggttgcgtga ctccggggtc gcaaggctcc acaatgagcg
     5701 cccgcagtgc cgcccgtcca gccccatccg ggactctccc gatgtacagg acggcgggac
     5761 cgcccacgcc tcgaacagga tgccgcgagc gaccgatgtc gaatccctca ccgcttcgca
     5821 ccgcgtcggg ctcgtcccgt cggccccccg agggcgcgct gctgcgctgc atcctggact
     5881 ccgaccgctg ggcggagagc ctgccgcccg tgccgtcgga ccgcctggtg acgctgtcgt
     5941 tcacgtccgc ctccgcgcag gtggagcacg ccgaggcctg cgagatgctg ggctaccgcg
     6001 tcgtgggcgc gccgccggac gccgcgccgc cggtcagcgt cgccgacctg ctggtcaccc
     6061 aggacatcat cgaggcgcac ccgacgtggt ggcgggcgct ggccacccac gccgaccgcg
     6121 tgtacagcct cgcgcacggc cccgccgtcg cgctgctggg cgacgtgctg gagccgcacc
     6181 tggccgcgcg cgcctgagcg cggcggctca gcgcgcggcg accttctgcc ccggcgacag
     6241 cgccaggccg tgccagaact tgcgcaggcg cacggaggtg ccgtcctcca gcgcgcggaa
     6301 ctccaggtcg tccacgagcg cgcgcatcag cagcagcccg cggccggact cgacctccgg
     6361 cccgaccgcc cggtccgtgc gcgggtcccg cgtgccgaag cccgggccca ggtccacgac
     6421 ctcgatctcg cagcagtcct ggtcgatgcg cagcaccacg gagtactcct ccgtgtccac
     6481 ggcgtggcgc accgcgttcg cgcacgcctc cgtcagcgcg agctggatgt cggcgctgac
     6541 atcctccggg cagccgagct cccggaacag cgactcggcg acgttgcggg cgacgcccac
     6601 gtagcgcacg tccctgggga gctggagttc cagtcgcagg agcatcgggc gtcgtcctcg
     6661 tccggggtct cgggcaagca gcggggcggc ggtggcaaat ctgctgatgc gcgcagtgtt
     6721 cccgcaggtc gacagggcat aaccgaggct cagtcacaac cgcgtgattc ttgcgcttcc
     6781 cgcccgtgcg cccagccccg ggagcaggct cagcggcgct tgccggagcg gccgccgtgg
     6841 gaggccgcca gctccaccag ctcctggagc gcctcgccca tgccctcgca gacgtcatcg
     6901 acgtccggca ccagcccgcg gtcggcgttc acgccgacga acacgtcctg ctcgtagctg
     6961 aacagcgtca cgttcagccc caccatctcg tggacggggc ccagcgggta gaacgcctcc
     7021 acgcgcgcgc cggcgcagta cagcgggaag cgcgggcccg gcacgttgga caccaccagg
     7081 ttccagatcg ggcggtgccg gtccgccgcg cgcgtgcgcg tgtacagtcg ggccgccagg
     7141 gacgccaccc cgcccggtgc cagctccgcg agctgctgca gcgtgttggc gcccagcgcg
     7201 ccgtgctgct ccttggcgcc cgccgtgctg gcgctgatgc ggcgcaggcg ctgcagcgtg
     7261 tcgggctcct ccacgggcag ggccgccagc atggccgaca cctggttgcc catctgcccg
     7321 cgctgctcct ccgcgcgcac ggagatcggc accatcgcga ccagggggcg ctccggcgcc
     7381 tcgtcccggc gcagcaggaa gcgccgcagc gagccggcca cgcacgccag caccacgtcg
     7441 ttgacggtcc cgcccagcgc gttcttgacg cgcttgaggt cctccagcgg catcgtcgac
     7501 agcgcgaccg cgcgccgggg gccgatcgag ccgttgaacg gcgtcgacgg cgccgtgaac
     7561 ggcgcgggcg gcggttcctc cttgcgcgtg cgcgccacct tgaacagcgc ggtggccgag
     7621 tcgaaggcct ggcggcccag ccgcgccacc gacagcggcg tggcggcctg gcgcagcccc
     7681 gcctgcacca gcagccgcag gtcgctcggc tcgcgctccg ctcgccacgg gcgcggcgcg
     7741 tccggcgcct cctggtccgg ggtgagctgc aggatcgccg cggccagctc cgcgcccgac
     7801 acgccgtcga tggcggcgtg gtgggtcttg gtgatcacgg cgacgcggcc gccctccagg
     7861 ccctccacca cccacagctc ccacaggggg tggttgcggt cgatcggccg gcccatcacg
     7921 tcgccggcga actcggccag ctcggccatt ccgcccggcg acggcagcgc cgcgcggcgc
     7981 acgtgcaggt ccaggtcgaa gtccgggtcc tcgatccaga ccgggtggtc aaggttgaac
     8041 ggcacccacg ccaggcggcg gcggaacggc gcggccaggt gcaggcgcgg ctccatgaag
     8101 cgcgccatgt cctcccgcgt cagcgacccg gacggcgtcg tcgacgggtc cagcaccagg
     8161 acaccgccca cgtgcatgtg gttggtcggc gtctccaggt acaggaacgc cgcgtcgaga
     8221 ccggtcaagc gggttggcac cgtgcaaacc cccaggtcgg gaaggttcgg cgcaccatac
     8281 tcgctagtct tcccggcacg aaggccgaaa cgagcacgcc acacaacgca cgactggaga
     8341 ggaacgccat gccggacgca ctggtcaccg cagactgggt cgccgagcac ctcgacgacc
     8401 cgcgggtcgt catcgccgag gtggacgagg acgccgccgc ctaccatgcc ggccacatcc
     8461 ccggcgccct cggattcgac tggcgcgccg acttccagga cccgctgaag cgcgccttcc
     8521 tggacgccga cggcttcgcc gccctgatgg actcgcgcgg catcacccgt gaccaccacg
     8581 tggtcctcta cggcggcaac aacaactggt tcgccgccta cgcctactgg tacttcaaga
     8641 tctacggcca ccccgcggtc agcctcatgg acggcggccg caagctgtgg gagctgcagg
     8701 gccggccgat gaccgacgag ccggtcaagc gcgaggccac cagcggctac caggccggcg
     8761 agccggacgc gtccatccgc gccaagcgcg accaggtgct cgccgagttc gtcggcgccc
     8821 cggacggcaa gggcctcatc gacgtgcgct ccccgcagga gttcgccggc gagctgatcg
     8881 ccccgccgca cctgcccaac gagggctcgc aggttcccgg ccacatcccc ggcgcgcaga
     8941 acatcccgtg ggccaaggcc gtggaccccg acaccgggca gttcctggac gccggcgcgc
     9001 tgaaggcgct gtacaccggc gtcgggatca cgccggacaa ggaggtcgtc gcgtactgcc
     9061 gaatcggcga gcgcagcgcg cactcctggt tcgtgctgca cgagatcctg ggctacccga
     9121 acgtgcgcaa ctacgacgga agctggaccg agtacgggtc cctggtggac gtgccggtcg
     9181 agcgctaggg gttcagagga gctccgggtc gggcccgcat cccgctgcgg ctctccaggg
     9241 aacccgccgc ccgggctagg tgccgttcgg tgccgcgggg cgtatccgca tcccgctgcg
     9301 gctctccagg ggacccgccg cccgggctct gcccgggcgg caccctgagt tcgcctcgcg
     9361 ggcgcggctc cgccccgccg tcatccgtcc agccactcgc cgagcacggc tagcagcttg
     9421 cggacgttgg cttcggtggc ggtgtggccc atgaggccga tgcgccaggc cctgccggcc
     9481 aacgcgccca gtccaccgcc cacctcgatg ccgtgctcgt ccagcagacg ccggcgccag
     9541 gccgcctcgt cgacgccatc gggcagcccc accgtggtga gctggggcag gcggtggccc
     9601 tcctgcgcca gcagcggcag gcccagcgcc tccaggccct cctgcagcat gcggccgacc
     9661 cgctcgtggc gggcgatgcg cgcgtccagt ccctcctcca ggaccacgcc gagtccggcg
     9721 tgcagcgcgt acagcatcga gatgggcgcg gtgtggtggt acttgcggcc gccctccgcc
     9781 gagacgtact cgaggatccc cgtcacgtcg aggtaccacg accggcacgg cgtccgccgc
     9841 gagcggatcc tctccgtggc gcgcggcccg aacgtcaccg gcgccaggcc gggcggcacc
     9901 gacaggcact tctgggtgcc gctgtaggcg gcgtccaccc cccagccgtc gatgtccacc
     9961 tcgacgccgc cgaggctggt gacgcagtcg atcgcgaaca gcgtctcgct gtcgcgcagg
    10021 gccgcggcga cctctgcgac cggctgccac acgccggtgg acgtctccgc gtgcaccagc
    10081 acggccaggg ccgggcggtg ctcgcggcag gccgccagga acgcctccgg gtcgacgatg
    10141 cgaccccagt cggcctccac ggcgtgcacc tcggccccgg cgcgccgggc cacctcggcg
    10201 aggcggccgc cgaagacgcc gttgacgccg accaccaccc ggtcacccgg ctcgaccagg
    10261 ttcaccacca cgcactccag gcccgcgctg ccggtgccgg acaccgggaa ggtcaccggg
    10321 ctggcggtgc ggaacacctg ccggagccgg tcgttgagct cgtccagcag cggcaggaac
    10381 tccgggtcca ggtgccccag gagcggctgg gacagcgcgg cgaggacgtc gggggcgaca
    10441 ttggagggcc cggggcccat gagaatgcgg tcggggatca tggcgcgcga tcctagtcca
    10501 caggatttgg ccgcttgtgg gtgcgttccg ggccagtcag cgcccagaac gcacccgact
    10561 tcggggggcg ggcgctgcta gcctcgggcg gtcgacgggc gagcgaggag agagcgatga
    10621 cgcaggtgat gccgggctgc gaagcctggt cggccgccgg gggcgaggtt ggagtcctgg
    10681 tgctccacgg cttcaccggc aacccggtca gcatgcgtcc ggtcgcggag gccatggccg
    10741 ccgccggcca cgccgtcgag atgccgcgcc tgcccggcca cggcaccacc tggcaggagt
    10801 gcaaccgcac cacctggcgg gactgggcgc gtgaggcagt ggcggcctac gagacgctgc
    10861 gcgcccgcac cggcgcgcag gtcgccgtgg gcctgtcgat gggcggcacc atggcgctgc
    10921 acctggcctc cacgcgcggc gacgacctgg ccggcgtggt cgcgatcaac ccgtcgctca
    10981 agtccagcga cccgcgcctg aaggcgctgc cggtgctcaa gcacctgatc cccggactga
    11041 agggcatcgg caacgacatc gccaagccag gcggcgacga gaagccctac cccaaggtgc
    11101 cgctcaaggc gctcgcctcc ttcctgcaac tgcagggcct ggtgcgcgag agcctgtccg
    11161 ccgtgacgat ccccacgctg gtgttcacgt cacgcaacga ccacgtggtg gagcccgaga
    11221 acggcgccct ggtcatcgac ggcatcgcct ccgccgactc cgaacaggtc tggctggagc
    11281 gcagctacca cgtcgcgaca cttgactacg acgccgacga gatcgtccag cgcacgctgg
    11341 cgttcatcaa gcgcgtcacc gcgaactgac cgcctcgccg gaacttcccc ccacgcgggg
    11401 gcgtcccacc ggcatgcgcg cattgacgat ggccctgctg ctgtcactgg cgctcgccgc
    11461 ctgcggcgag cccgccgcgc cgccctccga gtccgaggcg atccgctaca ccgccaccgc
    11521 aatggtcctg gacgacggcg gcgggcccga gctgtgcctg ggcgccgtcg ccacgtccct
    11581 gccgccgcac
//
LOCUS       DATMKC010000208         5177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_92668, whole genome shotgun sequence.
ACCESSION   DATMKC010000208 DATMKC010000000
VERSION     DATMKC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92668"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..353
                     /locus_tag="VNU01_06100"
     CDS             114..353
                     /locus_tag="VNU01_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWH32226.1"
                     /translation="MMAEQVLEYLAKALVDNPDDVEITTVEESDGELVLELRVHPDDM
                     GKVIGKRGRTAKALRTMIKAAATRDGASATVEIVE"
     gene            367..864
                     /gene="rimM"
                     /locus_tag="VNU01_06105"
     CDS             367..864
                     /gene="rimM"
                     /locus_tag="VNU01_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWH32227.1"
                     /translation="MEHPDLVAVGLVGKPHGLRGEVYVRLDPDFADPLTPGRVVATDR
                     HGDLTVTSTRDHSGRVVARFDGVATREAAEDLRGERLLLPRQEIELDEDAVWVDDLLG
                     LEVVTEEGELVGVVEAVTDGPAHDYLLVARPDGGEALIPVVDELVELDGERIVVRPVP
                     GLLDQ"
     gene            870..1571
                     /gene="trmD"
                     /locus_tag="VNU01_06110"
     CDS             870..1571
                     /gene="trmD"
                     /locus_tag="VNU01_06110"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWH32228.1"
                     /translation="MRIDVLSIFPEFFSGPLDASLIGKAREAGTLDVRVHDLRDWTHD
                     RHRTVDDLPFGGGAGMVMKPEPFFEAVEALYGSIEARPRTVLFTPRGRPLDQALAREL
                     SGEPALLLLCGRYEGIDERVHTGIATDEVSIGDYVLSGGEVAACVLVEAVTRLIPGVM
                     GNEASADDESFSDGLLEYPQYTRPADYRGMTVPDVLLSGHHANVAAWRREQAVARTRA
                     VRPDLYERLRREGEA"
     gene            1568..2293
                     /gene="lepB"
                     /locus_tag="VNU01_06115"
     CDS             1568..2293
                     /gene="lepB"
                     /locus_tag="VNU01_06115"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWH32229.1"
                     /translation="MSAALRTHVPPPHRSVRPPDLDSQRRAEIRGAEPPPRAKRSRRE
                     PSTLVLIVGALALAFLVKALLAQAFYIPSGSMIPTLLIGDRVIVEKLSYRFGEPQRGD
                     VVVFHRPGVERPTGPGQAVKEFLQGLGLLQPDADIDLIKRIVGLPGETIRIQGGVLFV
                     NGRALPEPYAVADTREFGPVTVPEGEYLVLGDNRPNSDDSRFSLGTVPRDHLVGKAFV
                     IIWPPGSARLGLSADYGRPVPAD"
     gene            2314..3156
                     /locus_tag="VNU01_06120"
     CDS             2314..3156
                     /locus_tag="VNU01_06120"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWH32230.1"
                     /translation="MAETLYTADANGVRHIVLARPEAYNTITPELRDELAAAIDAADA
                     DPDVRVMLLRAEGPAFCAGYGLDWSTEYQAGAESAERVWDSVTDMRLIGSFVDVYMKL
                     WYAKKPVIAAVQGWCIAGGTDMVLCADLIVAAEGASFGYPPARVWGTPTTAMWVYRMG
                     IEQAKRYLLTGDEIPAPKAAEIGLVLETVPDERLLEHATALAERMARLPESQLVMLKL
                     LCNQTVENMGLASSRTLGTLFDGIARHTQEGLDFVSRSMEVGIRQAVRERDDPFQDYG
                     SRPR"
     gene            complement(3222..3863)
                     /locus_tag="VNU01_06125"
     CDS             complement(3222..3863)
                     /locus_tag="VNU01_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWH32231.1"
                     /translation="MLALLLTACGAPVGQAPAPSPSPQFHSRILHIWPELEAVLRAYS
                     WRPGCPVPIRDLRYIQVSHWTFDGRIATGEVIVHEGVTGQAVGLFADLFQLGFPIAQM
                     RRIDDFAGSDDASMAANNTSGFNCRKVAGTDRWSEHAYGRAIDVNPVQNPYVRGSTVQ
                     PAAGRAYLDRSAYRPGMLIAGRPEVAAAKRHSWGWGGDWTSSKDYQHLSTTGR"
     gene            4099..4458
                     /gene="rplS"
                     /locus_tag="VNU01_06130"
     CDS             4099..4458
                     /gene="rplS"
                     /locus_tag="VNU01_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWH32232.1"
                     /translation="MNPTDLVDVAYLRDDVPDFRPGDTVKVHVKVTEGTRTRIQVFEG
                     DVIARRGSGIRESFTVRKISFNAIGVERTFPVHAPVIDHIEVMRRGKVRRAKLYYLRD
                     RVGKKAKIKELREPAGA"
     gene            4481..>5177
                     /gene="lepB"
                     /locus_tag="VNU01_06135"
     CDS             4481..>5177
                     /gene="lepB"
                     /locus_tag="VNU01_06135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWH32233.1"
                     /translation="MEEHTTRPLDDVAEPAAPAKRSFLSELPVLVIVAFVLALVLKTF
                     LVQAFYIPSSSMEPTLQIGDRVLVNKLAHTFREPRRGEIVVFSVDGGTPAMVEPANPV
                     VRFLRGLASGLGMAPASDRDFIKRVIGLPGDVVEMRDGVVTVNGVALPEAPASEGGYL
                     SERHMEPFGPFTVPEGHYFMMGDNRPNSSDSRYSLGPIAEDRIVGRAFVIIWPLSHAG
                     MLTLPDYGEIPVVG"
BASE COUNT          732 a         1816 c         1906 g          723 t
ORIGIN      
        1 gccgaggagc ccgccgccga ggagcccgcc gccgaggaga agcccgccgc cgagcagccc
       61 gccgccgagg aggcagtggc cgaggagccg gcgcccgagc cggaggaagc ggaatgatgg
      121 cggaacaggt gctggagtac ctggccaagg cgctcgtcga caatcccgac gacgtggaga
      181 tcacgacggt cgaggagtcc gacggcgagc tggtcctgga gctgcgggtc cacccggacg
      241 acatgggcaa ggtcatcggc aagcgcggcc gcaccgccaa ggcgctgcgg accatgatca
      301 aggccgccgc gacccgtgac ggcgcctcgg ccacggtcga gatcgtcgag tagcgcgctc
      361 cgcgcgatgg agcacccgga cctcgtcgcg gtcgggctcg tgggcaagcc ccacggcctg
      421 cgcggcgagg tctacgtgcg cctggacccg gatttcgccg acccgctcac accggggcgc
      481 gtggtcgcca ccgaccggca cggcgacctg accgtcacct cgacccgcga ccacagcggg
      541 cgcgtggtcg cccggttcga cggcgtcgcc acgcgcgagg cggcggagga cctgcgcggc
      601 gagcgcctgc tgctgccgcg ccaggagatc gagctggacg aggacgccgt gtgggtcgac
      661 gacctgctcg gcctggaggt cgtgaccgag gagggggagc tggtgggcgt cgtggaggcc
      721 gtgaccgacg ggccggcgca cgactacctc ttggtcgccc ggcccgacgg cggcgaggcg
      781 ctgatccccg tggtggacga gctggtggag ctggacggcg agcgcatcgt cgtgcgcccg
      841 gtccccggat tgctggacca gtagccgcca tgcgcatcga cgtgctgtcc atcttccccg
      901 agttcttcag cggcccgctg gacgcgtccc tcatcggcaa ggcgcgcgag gccggcacgc
      961 tggacgtgcg tgtgcacgac ctgcgcgact ggacgcacga ccgccaccgc accgtcgacg
     1021 acctgccgtt cggcggcggc gccggcatgg tcatgaagcc ggagccgttc ttcgaggccg
     1081 tcgaggccct gtacggctcg atcgaggcgc ggccgcgcac ggtgctgttc acgccgcgcg
     1141 gccgcccgct ggaccaggcg ctggcgcgcg agctgtcggg cgagccggcg ctgctgctgc
     1201 tgtgcggccg ctacgagggc atcgacgagc gcgtgcacac cggcatcgcc accgacgagg
     1261 tctccatcgg cgactacgtg ctgtccggcg gcgaggtggc cgcctgcgtg ctggtggagg
     1321 cggtcacgcg cctgatcccc ggcgtcatgg gcaacgaggc cagcgccgac gacgagtcgt
     1381 tcagcgacgg gctgctggag tacccccagt acacccgtcc ggccgactac cgcggcatga
     1441 ccgtcccgga cgtgctgctg tccggccacc acgccaacgt ggcggcctgg cggcgcgagc
     1501 aggccgtggc gcgcacgcgc gccgtgcggc cggacctgta cgagcggctg cggcgggagg
     1561 gcgaggcgtg agcgccgcgc tgcgcaccca tgtgccgccg ccgcaccgct cggtgcgccc
     1621 gccggacctg gacagccagc gccgggccga gatccgcggg gccgaaccgc cgccgcgcgc
     1681 caagcggtcg cggcgcgaac cgagcacgct ggtgctgatc gtgggcgccc tggcgctggc
     1741 gttcctggtg aaggcgctgc tggcgcaggc gttctacatc ccgtccggct ccatgatccc
     1801 gacgctgctg atcggcgacc gcgtcatcgt ggagaagctc agctaccgct tcggcgagcc
     1861 gcagcgcggc gacgtcgtgg tgttccaccg gccgggggtg gagcggccca ccgggccggg
     1921 gcaggcggtg aaggagtttc tccagggcct ggggctgctg cagccggacg ccgacatcga
     1981 cctcatcaag cgcatcgtgg ggctgcccgg cgagacgatc cggatccagg gcggtgtgct
     2041 gttcgtgaac gggcgggcgc tgccggagcc gtacgcggtg gcggacacgc gcgagttcgg
     2101 ccccgtgacg gtgcccgagg gggagtacct tgtcctgggc gacaaccggc ccaacagcga
     2161 cgacagccgc ttctcgctcg gcacggtgcc gcgcgaccac ctggtcggca aggcgttcgt
     2221 gatcatctgg ccgccgggca gcgcgcggct gggcctgtcg gcggactacg ggcggcctgt
     2281 cccggccgac tgagcgtcct ctaggctgcg gccatggcgg agaccctgta cacggcggac
     2341 gccaacggcg tgcgccacat cgtgctggcg cggcccgagg cgtacaacac gatcactccc
     2401 gagctgcgcg acgagctggc cgcggcgatc gacgcggccg acgcggaccc ggacgtgcgc
     2461 gtgatgctgc tgcgcgcgga ggggccggcg ttctgcgcgg gctacgggct ggactggtcg
     2521 accgagtacc aggcgggggc cgagtccgcc gagcgcgtgt gggactcggt caccgacatg
     2581 cgcctgatcg gcagcttcgt cgacgtgtac atgaagctgt ggtacgcgaa gaagccggtg
     2641 atcgccgccg tgcagggctg gtgcatcgcc ggcggcacgg acatggtgct gtgcgcggac
     2701 ctgatcgtgg cggccgaggg cgcgagcttc ggctacccgc ccgcgcgggt ctggggcacc
     2761 ccgaccaccg cgatgtgggt ctaccggatg ggcatcgagc aggcgaagcg ctacctgctc
     2821 accggcgacg agatccccgc gccgaaggcc gccgagatcg gcctcgtcct ggagaccgtg
     2881 cctgacgagc ggctgctgga gcacgccacc gcgctggccg agcgcatggc gcggctgccg
     2941 gagagccagc tggtgatgct gaagctcttg tgcaaccaga ccgtggagaa catgggcctg
     3001 gcgtcctcgc gcaccctcgg cacgctgttc gacgggatcg cccggcacac ccaggagggg
     3061 ctggacttcg tcagccgctc gatggaggtg ggcatccgcc aggccgtgcg cgagcgcgac
     3121 gaccccttcc aggactacgg gtcgcgcccc cgctaacccc gccttttccc ccgagttgga
     3181 agatcaggcc cgcatatggg ccagatcttc caacttcctg gtcagcggcc ggtggtggac
     3241 aggtgctggt agtccttgct ggaggtccag tcgccgcccc agccccagct gtgccgcttc
     3301 gccgcggcga cctccggccg gccggcgatc agcatgccgg gccggtacgc cgagcggtcc
     3361 aggtacgccc gccccgccgc cggctgcacc gtggacccgc gcacgtaggg gttctgcacc
     3421 gggttgacgt cgatggcgcg cccgtaggcg tgctccgacc agcggtccgt cccggccact
     3481 ttgcggcagt tgaaaccgga ggtgttgttg gccgccatcg acgcgtcgtc gctgccggcg
     3541 aagtcgtcga tgcggcgcat ctgggcgatc gggaagccga gttggaacag gtcggcgaac
     3601 agtcccaccg cctgcccggt cacgccctcg tgcacgatca cttcgccggt ggcgatgcgc
     3661 ccgtcgaagg tccagtggga cacctggatg tagcgcaggt cccggatcgg cacggggcag
     3721 ccggggcgcc acgagtacgc gcgcagcacc gcctccagtt ccggccagat gtgcaggatc
     3781 cgcgagtgga actgcgggct gggcgacggc gccggggcct ggccgactgg tgcgccgcaa
     3841 gcggtcagga gcagcgccag caccagcgcg aagcctgtgg attggaggat tcggcccata
     3901 tacgggcctg atcttccaac tcggggaaag ggcgcacaag ggggcggggc ggcaagggca
     3961 gcgcaaaggc cgggtcgtgg gccggattgc cgcccgccag ttgcccggtt atgctggcca
     4021 accggtgctg cgccgccgta gctcgtgcgc gcccgctcgt gttttttccg ttgtgacacc
     4081 cgcccgcgga ggcctgaagt gaacccgact gacctcgttg acgtcgccta cctgcgcgat
     4141 gacgtgccgg actttcgtcc cggcgacacc gtgaaggtgc acgtcaaggt gaccgagggc
     4201 acccgcacgc gcatccaggt cttcgagggc gacgtgatcg cccgccgcgg cagcggcatc
     4261 cgcgagtcct tcacggtccg caagatcagc ttcaacgcga tcggcgtgga gcgcaccttc
     4321 ccggtgcacg cccccgtcat cgaccacatc gaggtcatgc gccggggcaa ggtgcgccgc
     4381 gcgaagctgt actacctgcg cgaccgtgtc ggcaagaagg ccaagatcaa ggagctgcgc
     4441 gagcccgccg gcgcctgacg cgacggcgct cgccgccgcg atggaggagc acaccacgcg
     4501 cccgctcgac gacgtggccg agccagccgc gccggccaag cgcagcttcc tttcggagct
     4561 gccggtcttg gtcatcgtgg cgttcgtcct tgccctggtc ctgaagacct tcctggtcca
     4621 ggccttctac atcccgtcgt cgtccatgga acccacgctc cagatcggcg accgggtgct
     4681 ggtgaacaag ctggcgcaca cgttccgtga gccccgccgc ggcgagatcg tcgtgttctc
     4741 cgtggatggc ggcacgcccg ccatggtgga gccggccaac ccggtcgtgc gcttcctgcg
     4801 cgggctcgcc tccggcctgg gcatggcgcc cgccagcgac cgcgacttca tcaagcgcgt
     4861 catcggcctg cccggcgacg tcgtggagat gcgcgacggg gtggtcacgg tgaatggcgt
     4921 ggccctgccc gaggcgcccg catccgaggg cggctacctg tcggagcgcc acatggagcc
     4981 gttcgggccg ttcaccgtgc ccgagggcca ctacttcatg atgggcgaca accgcccgaa
     5041 ctcctccgac agccgctaca gcctgggccc gatcgcggag gaccgcatcg tgggccgggc
     5101 cttcgtcatc atctggccgc tgtcgcacgc cggcatgctg acgctgcccg actacgggga
     5161 gatccccgta gtcgggc
//
LOCUS       DATMKC010000209         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_75451, whole genome shotgun sequence.
ACCESSION   DATMKC010000209 DATMKC010000000
VERSION     DATMKC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75451"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VNU01_06140"
     CDS             complement(<1..210)
                     /locus_tag="VNU01_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32234.1"
                     /translation="MEALRAAVDGVLSRDPHQLADAELSAELAELRRQADRIEAAFLA
                     RLAVFDARRAYADERAANAAVWLRNN"
     gene            complement(300..869)
                     /locus_tag="VNU01_06145"
     CDS             complement(300..869)
                     /locus_tag="VNU01_06145"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HWH32235.1"
                     /translation="MSLVATVVLLAVAIGYAAGGRLRHIAQIPFRRIWMVWIAFLLQF
                     LLPFLDESTRRTLRVPILAVAFVLAMWWIVGTWPVLTRWLRAGLVLVVVGWAMNFTVI
                     ALNNGMPVSAETLDRVGLPSDNIESGDLNKHVTLDDDTLLPWLGDMIPLPLAGPLRKA
                     ISLGDVVMLLGLLVFIPVGMRQEPDEATA"
     gene            972..2573
                     /locus_tag="VNU01_06150"
     CDS             972..2573
                     /locus_tag="VNU01_06150"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWH32236.1"
                     /translation="MHPYVVEQFTDEERRFLSRFVTNTDRPVFGLVNLPEVVKGALFA
                     RYSRSRKSLRRLLLDEFRDDLEGLEVDVDRTAADRAERLYQRVFDEYGDDSVAQLGGA
                     HVAVEQASNLLTKVLEWGRLGAYLEQSTRYMRYDDKPGGRYRYYVDPDVAASPLGETY
                     RREMDATFDGYSALFEPVLAWARERFPQEDGVSDAVYRNTLNAKACDVLRGLLPAATV
                     SNVGIFATGQAYENLLLRLRSHELAEAREVGDLLLAELRQVIPSFLVRVDRPDRGGVW
                     SDYLRATRDATRAVADEVLGGEKPADAPEVALVDWDDDAETDIVVAMLYPYSDLPEAQ
                     LRRLVEDMPSAERTRVVEAYAGERRNRRHRPGRALERAFYRFDVLGDYGAFRDLQRHR
                     MLTIEWQELGCRHGYDVPPEIVEAGAEDDWHRIMERQARLWESLRADFPRQAQYAVGL
                     AWKLRYSMNLNARAAMQMLELRTTPQGHPSYRRVAQEMHRQIGEVAGHHAIAAMMRFV
                     DYGQAELERLEAEKASEAKRAARGA"
BASE COUNT          401 a          933 c          926 g          330 t
ORIGIN      
        1 gttgttgcgc agccacaccg cggcgttggc cgcccgctcg tcggcgtagg cgcggcgggc
       61 gtcgaacacc gccaggcgcg cgaggaacgc cgcctcgatg cggtcggcct gccggcgcag
      121 ttcggccagc tcggcggaca gctcagcgtc ggcgagctga tgcgggtcgc ggctcaggac
      181 cccgtcgacg gcggcacgca acgcttccat gccgcccaca ctcccacgag ggtgtgacga
      241 aaaccggccc cgaaacgcac ttgcgcacag gccacaggaa acttttccga gaaaatctct
      301 caggcggtgg cttcgtcggg ctcctggcgc atgcccaccg ggatgaacac cagcaggccc
      361 agcagcatca ccacgtcgcc caggctgatc gccttgcgca ggggcccggc cagcggcagc
      421 gggatcatgt cacccagcca cggcagcagc gtgtcgtcat ccagggtcac gtgcttgttg
      481 aggtcgccgg actcgatgtt gtccgacggc aggccgacgc ggtccagggt ctcggcggac
      541 accggcatgc cgttgttcag cgcgatcacg gtgaagttca tcgcccagcc gaccacgacc
      601 agcaccagcc cggcgcgcag ccagcgggtc agcacgggcc aggtgccgac gatccaccac
      661 atcgccagca cgaacgcgac cgccaggatc ggcacgcgca gcgtccggcg ggtggactcg
      721 tccaggaacg gcagcaggaa ctgcagcagg aacgcgatcc acaccatcca gatgcggcgg
      781 aacgggatct gcgcgatgtg gcgcagccgt ccgccagcgg cgtagccgat cgccacggcc
      841 agcagcacca cggtcgcgac caggctcatt tgcgttccct ttccccgacc ccccggcgcc
      901 cgccggtggg agcgctcatt gtgccgtgcc ggtccggccg gggcgtctca ggcggtgcgt
      961 acactgcccc gatgcacccc tacgtcgtcg agcagttcac cgacgaggaa cggcgtttcc
     1021 tcagccggtt cgtcacgaac accgaccggc cggtcttcgg cctggtcaac ctgccggagg
     1081 tcgtgaaggg cgcgctgttc gcccggtact cgcgcagccg caagagcctg cggcggctgc
     1141 tgctggacga gttccgcgac gacttggagg gcctggaggt cgacgtcgac cgcacggcgg
     1201 ccgaccgcgc ggaacggctg taccagcgcg tgttcgacga gtacggcgac gactcggtgg
     1261 cgcagctcgg cggcgcgcac gtggccgtgg agcaggccag caacctgctc accaaggtgc
     1321 tggagtgggg ccggctgggc gcctacctgg agcagtccac gcgctacatg cgctacgacg
     1381 acaagcccgg cgggcgctac cgctactacg tggaccccga cgtggccgcg tcgccgctcg
     1441 gcgagaccta ccgccgcgag atggacgcca ccttcgacgg ctactcggcc ctgttcgagc
     1501 ccgtcctggc gtgggcgcgc gagcgcttcc cgcaggagga cggcgtcagc gacgcggtgt
     1561 accgcaacac gctcaacgcc aaggcctgcg acgtgctgcg cggcctgctg cccgccgcga
     1621 ccgtgagcaa cgtcggcatc ttcgccaccg ggcaggccta cgagaacctg ctgctgcgcc
     1681 tgcgctcgca cgagctggcc gaggcgcgcg aggtcggcga cctcctcctg gcggagctgc
     1741 gccaggtgat cccgagcttc ctggtgcgcg tcgaccgccc ggaccgcggg ggcgtctgga
     1801 gcgactacct gcgcgccacg cgcgacgcca cgcgcgccgt ggccgacgag gtgctgggcg
     1861 gggagaagcc ggccgacgcg ccggaggtgg cgctggtcga ctgggacgac gacgccgaga
     1921 ccgacatcgt ggtggcgatg ctgtacccgt acagcgacct gccggaggcc cagctgcgcc
     1981 gcctggtgga ggacatgccc tcggcggagc gcacccgcgt ggtggaggcc tacgccggtg
     2041 agcggcgcaa ccggcgccac aggccgggcc gcgccctgga gcgcgcgttc taccgcttcg
     2101 acgtcctggg cgactacggc gcgttccgcg acctgcagcg ccaccgcatg ctgaccatcg
     2161 agtggcagga gctgggctgc cgccacggtt acgacgtgcc gccggagatc gtcgaggcgg
     2221 gggccgagga cgactggcac cgcatcatgg agcgccaggc gcggctgtgg gagtcgctgc
     2281 gggccgactt cccgcggcag gcgcagtacg ccgtgggcct ggcgtggaag ctgcgctact
     2341 ccatgaacct caacgcgcgc gccgccatgc agatgctgga gctgcgcacc acgccgcagg
     2401 gccaccccag ctaccggcgc gtcgcccagg agatgcaccg ccagatcggc gaggtcgccg
     2461 gccaccacgc catcgcggcg atgatgcgct tcgtcgacta cggccaggcg gagctggagc
     2521 ggctggaggc ggagaaggcc agcgaggcca aacgcgccgc ccgcggcgcc tgactcagcc
     2581 ggccaggggc
//
LOCUS       DATMKC010000210         7942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_275594, whole genome shotgun sequence.
ACCESSION   DATMKC010000210 DATMKC010000000
VERSION     DATMKC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275594"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..445
                     /locus_tag="VNU01_06155"
     CDS             188..445
                     /locus_tag="VNU01_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32237.1"
                     /translation="MEAAVLACDALCIFARGEYSVGQDHGQALQTLRSVEGGAALSKA
                     LGTALQAKKQHAYEVGLPKNSEMVALRRAAERLVQVAESRM"
     gene            647..820
                     /locus_tag="VNU01_06160"
     CDS             647..820
                     /locus_tag="VNU01_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32238.1"
                     /translation="MSAPRGAGRSGGLLFLLILAFVAYLVLEALAGLAQVVTGVLLLA
                     VIVVLAVNVLRRR"
     gene            856..2019
                     /locus_tag="VNU01_06165"
     CDS             856..2019
                     /locus_tag="VNU01_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32239.1"
                     /translation="MSLSFALTPEQEELRKLVRQFADERVAPAAEAFNAEGRFPTELI
                     LEMGQMGLFGLPFPESYGGSGGDFVSLCLAIEELGRVDQSVGITLEAAVGLGANPIWK
                     FGSEEQKRELLPPLARGERLAGFGLTEPGGGSDAGGLRTRAVLDGDHWVINGSKQFIT
                     NVGTDISAFVTITAITGDRDGKPEVTNIVVPTGTPGYTIGNGYRKVGWHASDTRDLYF
                     SDCRVPAGNQLGERGRGFGNFLAILDEGRVAISALAVGLIQGCVEECSRYAHERESFG
                     RPIGSYQAVAFKIADMEMQADIGRTAYLRAAWLAQEGKPFKKEAAAVKLFTTEAAVDA
                     ARQATQIFGGYGFTTEYRVGRFYQDAKILEIGEGSSEVQRLILARHLGLPDPR"
     gene            complement(2120..3316)
                     /locus_tag="VNU01_06170"
     CDS             complement(2120..3316)
                     /locus_tag="VNU01_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWH32240.1"
                     /translation="MRRNVKELLVEAKDASELMVDLAYAAVFFGDDDLAREVIRLEER
                     MDAAVHELRILCMLATRSPEDAEQLAGILGMVNAVEEIADAAEDIARVVLRDLGVPRE
                     LRDDLRHAEEVVARVKIREDNRLAGRPLWELALPSETGMWVIAVRRDMDYLYGPSGDT
                     VLQEGDVLFLQGPPDGVDLARELAGGEPYGLKPPGEGGRLSNLDRAVDILVELKNAAE
                     VAVGLAYSAILFRDLGLAREVSVLEDKCDALYHDLQRWVLRAAGDVDDDGIDELRGLL
                     QIGLSSERITDAAQEMTRLAESEDEPHPVIAAALRETGEIVTDALVNAGGPGEGRTLQ
                     ELRLETETGMYVLAIQREQRWLYRPRGTTRLIAGDRLLCTGPEEGVALLRDLTGDPRD
                     PETHED"
     gene            complement(3319..4548)
                     /locus_tag="VNU01_06175"
     CDS             complement(3319..4548)
                     /locus_tag="VNU01_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWH32241.1"
                     /translation="MAIRGKAAREVLAYWAQERRTLRQGFAALAISTGVGMGAGVVLA
                     HMEGLLGQLPGLLLLVPAAIGMRGAIFGALAARLGTGIMTGQFTPSWDRRSFAVQNVE
                     ASALLTLFSSALAAVAARVVAGAFGLDTISLWKLMTISMVGGILSSAVILGVVLVLAR
                     TAQRREWDMDAIGAPLVTTVGDIVTLPALVIGTLLVSSDTVSAIAGVGLLLLGAYGVW
                     QGTRSEGPLARRIVTESMPVLLYAAAVDILAGGVLETRVHEFVANPALLVLVPPFIAN
                     CGSLGGILSARLGSDLHLGLVEPRRLPQKQALLEGTLTVLFSVLAFSGVGLIAHVASL
                     LFGFASPGLAAMVGVALAGGGLALVLMFLVAYYAATATYRFGLDPDNHGIPIVTATMD
                     FFGVLCLVAAIVLFGVT"
     gene            4587..5756
                     /locus_tag="VNU01_06180"
     CDS             4587..5756
                     /locus_tag="VNU01_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWH32242.1"
                     /translation="MTQEPRVSDLFTLTDDHRAFREVVRKMAEERIAPRAAEIDETAE
                     FPHDVKDLLEQQGLIGLFVPESYGGAGADALTVAILVEEIARVCASSSLIPLVQKLGC
                     MPIVLGASEEQKKAWLPKLATGEELISYAISEAEAGSDPASMRTTAVRDGDSYVLNGT
                     KVWISGASVADCFTVLAKTDPGSGAKGVSAFYVRADDAGFSVGKKEDKLGIKGSPTCQ
                     IHFDNVRVPADRLIGEEGKGFTYAMQAFDHTRLAVGAQAVGIAQGALDFAAGYVKERQ
                     QFGKPIGSFQGLQFMIADMATDIDAARALVYIAAGKADRGDEDLTRWSSMAKLKAGDV
                     AMRVTVDAVQLLGGYGYTKDYPVERYMRDAKITQIYEGTQQIQRMVIARQVLGRL"
     gene            5761..6333
                     /gene="msrA"
                     /locus_tag="VNU01_06185"
     CDS             5761..6333
                     /gene="msrA"
                     /locus_tag="VNU01_06185"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWH32243.1"
                     /translation="MSDRHAVLGTPMHPPFPEGLERAVFGMGCFWGAEELYWQVDGVF
                     TTAVGYAGGETAGPSYELVCTGRTGHAEVVLVVFDPARVSYAELLKMFWEQHDPTQGM
                     RQGNDIGTQYRSLIQVHSAEQRRTAEASRDAYQQALNKAGRGAITTEITGATEFWYAE
                     PSHQQYLAKNPHGYRCHARTDVPFPAGVGS"
     gene            6330..6878
                     /locus_tag="VNU01_06190"
     CDS             6330..6878
                     /locus_tag="VNU01_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWH32244.1"
                     /translation="MSSPQEPHRDGRRVADSEVTFARTMTQLDANLVGKVHGGVIMRE
                     VDSAAGTAASRHAGRVCVTAAIDELSFLQPVEVGDILIVKARVNYVGRTSMEVGVRVE
                     AEPWTGGERRHTTSAYLTMVALGQDGKPVEVPPLVPETDEERRRCERARIRREMRKER
                     IERLGCYGPGPVTAQADDTPAG"
     gene            6882..7445
                     /locus_tag="VNU01_06195"
     CDS             6882..7445
                     /locus_tag="VNU01_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HWH32245.1"
                     /translation="MEDFALAYGVALVAELGDKSQLAAASFGARHRVLPVLLGFTLGA
                     AAALGLGVLLGAAVGELLAPEARSVVAGTLFLAFAAWSLRSDDRDEQASAAVRARVVF
                     AGVALAVFVAEMGDKTQFATAALAARQAALPTWLGATLGEVTASGAAILVGAWLGPRM
                     PERALRIGGAVVFAAVGIAMLADGLVG"
     gene            7446..7802
                     /locus_tag="VNU01_06200"
     CDS             7446..7802
                     /locus_tag="VNU01_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HWH32246.1"
                     /translation="MRRLGLVAAGGAAGAFVRAVVADVVPRTGGLPLATLLVNLAGAF
                     ALGLLLGRVGEDPRHGALPRALIGVGFLGALTTFSALAVELALMPVPAAAGYGLATLA
                     GGLALARTGLRLGRAR"
BASE COUNT         1119 a         2883 c         2883 g         1057 t
ORIGIN      
        1 cgcaacgtgc tgcgtgacgg gcgtgtcctc tccggcgagc cgctgaccgc ggatgagtag
       61 acgggcccag tcaaggcccg cggacagagt ccggggacgg gccgcggcaa ccaaagcccg
      121 ccggctcctc gacggtggga tccgcgcgca tgacaggggc gacatcgaca ccgcgttggg
      181 ctctttcgtg gaggccgcag tgctcgcttg tgacgctctg tgcatcttcg cccgagggga
      241 gtactccgtc ggccaggacc acggccaggc cctccagaca ctgcggtcgg tcgagggcgg
      301 cgcagcgctg tcgaaggccc tcggcacggc tctccaggcg aagaagcagc acgcctatga
      361 ggtgggactt cccaagaact cggaaatggt cgccctgcgc cgggcggccg agaggctggt
      421 gcaagtcgcc gagtctcgaa tgtgagaacg actgagggcc ggtggggggg accggccctc
      481 agcgtgctca tgggatggag cgaggtgttt gtttgtgacg gccgtgtcgc gggcgcgggg
      541 gcgacgcccc accggcgcgt cagatgtgat cctgcgacgg cgggaaccgg cataaaccgg
      601 gcgcgcggaa tctttacgga gcggccgcgc ggctaccatc cggcgcatga gcgcaccccg
      661 cggagccggc cgtagtggcg gcctgctgtt cctgctgatc ctggccttcg tggcctacct
      721 ggtgctggag gcgctggccg gactggcaca ggtcgtgacc ggcgtcctgc tcctggcggt
      781 gatcgtggtg ctcgccgtca acgtgctccg ccgccgctag ctacgatcct gcccccgtca
      841 ccggaggttg ccgccgtgtc gctgtcgttc gccctcacgc ccgagcagga ggagctgcgc
      901 aagctggtgc gccagttcgc cgacgagcgg gtcgctcccg ccgccgaggc gttcaacgcg
      961 gaagggcgtt tccccacgga gctgatcctg gagatgggcc agatgggcct gttcggcctg
     1021 cccttccccg agtcctacgg cggctccggc ggcgacttcg tctcgctgtg cctggcgatc
     1081 gaggagctcg gccgggtcga ccagtccgtg ggcatcacgc tggaggcggc cgtcggcctg
     1141 ggcgccaacc cgatctggaa gttcggcagc gaggagcaga agcgcgagct gctgccgccg
     1201 ctggcgcgcg gcgagcggct cgccggcttc ggcctgaccg agccgggcgg cggctccgac
     1261 gccggcggcc tgcgcacgcg cgccgtgctg gacggcgacc actgggtgat caacggctcc
     1321 aagcagttca tcaccaacgt cggcacggac atctccgcct tcgtcaccat caccgcgatc
     1381 accggcgacc gcgacggcaa gccggaggtc accaacatcg tggtgccgac gggcacgccc
     1441 ggctacacga tcggcaacgg ctaccggaag gtgggctggc acgcctcgga cacgcgcgac
     1501 ctgtacttct ccgactgccg cgtgcccgcg ggcaaccagc tgggggagcg cggccgcggc
     1561 ttcggcaact tcctggcgat cctggacgag ggccgcgtgg cgatctcggc gctggcggtt
     1621 gggctgatcc agggctgcgt ggaggagtgc agccgctacg cccacgagcg cgagtccttc
     1681 ggccggccca tcggcagcta ccaggcggtg gcgttcaaga tcgccgacat ggagatgcag
     1741 gccgacatcg gccgcaccgc ctacctgcgc gcggcgtggc tggcgcagga gggcaagccg
     1801 ttcaagaagg aggccgcggc ggtcaagctg ttcaccaccg aggcggcggt cgacgcggcg
     1861 cgccaggcca cgcagatctt cggcggctac ggcttcacca ccgagtaccg ggtgggccgc
     1921 ttctaccagg acgccaagat cctcgagatc ggcgagggct cctccgaggt gcagcgcctc
     1981 atcctcgccc gccacctcgg cctgcccgac ccccgctgac cccccgctcg caacggttca
     2041 cagtgtgcag ttccccccgt atacgaggga aagcgcacac tgtgaaccgt tttggggctg
     2101 agggcgcggc gaggactgct cagtcctcgt gggtctcggg gtcgcggggg tcgccggtga
     2161 ggtcgcgcag gagggcgacg ccctcctcgg ggccggtgca cagcaggcgg tcgcccgcga
     2221 tgaggcgcgt ggtgccgcgc ggccggtaca gccagcgctg ctcgcgctgg atcgccagca
     2281 cgtacatccc cgtctcggtc tccagccgca gctcctgcag cgtgcgtccc tcaccggggc
     2341 cgccggcgtt caccagcgcg tcggtgacga tctcgccggt ctcccgcagc gccgccgcga
     2401 tcaccgggtg cggctcgtcc tcgctctcgg ccagccgcgt catctcctgc gccgcgtcgg
     2461 tgatgcgctc cgacgacagg ccgatctgca gcagcccgcg cagctcgtcg atgccgtcgt
     2521 cgtccacgtc gcccgccgcc ctgaggaccc agcgctgcag gtcgtggtac agcgcgtcgc
     2581 acttgtcctc cagcacgctc acctcgcgcg ccaggcccag gtcgcggaac aggatcgcgg
     2641 agtaggccag gccgaccgcc acctcggcgg cgttcttgag ctccaccagg atgtccaccg
     2701 cgcggtccag gttggacagg cgcccgccct cgcccggcgg cttgaggccg tacggctcgc
     2761 cgcccgcgag ctcgcgcgcc aggtcgacgc cgtccggcgg cccctggagg aacagcacgt
     2821 cgccctcctg cagcaccgtg tcgccactcg gcccgtacag gtagtccatg tcgcggcgca
     2881 ccgcgatgac ccacatgccg gtctcgctgg gcagcgccag ctcccacagc ggcctgccgg
     2941 cgaggcggtt gtcctcgcgg atcttcaccc gcgccaccac ctcctcggcg tggcgcaggt
     3001 cgtcgcgcag ctcgcgcggc acgccgaggt ctcgcagcac cacgcgcgcg atgtcctcgg
     3061 cggcgtcggc gatctcctcg acggcgttga ccatgcccag gatgccggcg agctgctccg
     3121 cgtcctcggg cgagcgggtc gcgagcatgc acaggatgcg cagctcgtgc accgcggcgt
     3181 ccatgcgctc ctcgaggcgg atcacctccc gcgcgaggtc gtcgtcgccg aagaacaccg
     3241 ccgcgtaggc gaggtccacc atcagctccg aggcgtcctt cgcctcgacg agcagctctt
     3301 tgacgttgcg tcgcatagtc aggtcacccc gaacaggacg atggccgcca ccaggcacag
     3361 gaccccgaag aagtccatgg tggcggtcac gatggggatc ccgtggttgt cgggatccag
     3421 gccgaagcgg tacgtcgcgg tcgccgcgta gtaggcgacc aggaacatca ggaccagcgc
     3481 cagacccccg cccgccaggg cgacgccgac catggcggcg agtcccggcg aggcgaagcc
     3541 gaacagcaac gacgcgacgt gggcgatgag ccccacgccg ctgaacgcga gcacgctgaa
     3601 cagcacggtg agcgtgccct ccagcagcgc ctgcttctgc ggcaggcggc gcggctccac
     3661 caggcccaga tgcaggtcgc tgcccaggcg cgcggacagg atgccgccca gggagccgca
     3721 gttggcgatg aacggcggca ccagcaccag cagcgccggg ttggccacga actcgtgcac
     3781 gcgcgtctcc agcacgcctc cggccaggat gtcgaccgcg gccgcgtaga gcaggaccgg
     3841 catgctctcc gtcacgatgc gccgggccag cgggccctcg ctgcgcgtgc cctgccagac
     3901 gccgtacgcg ccaagcagca gcaggcccac cccggcgatc gcgctcaccg tgtcactgga
     3961 caccagcagc gtgccgatga ccagtgccgg gagcgtcacg atgtcgccca cggtggtcac
     4021 cagcggcgcg ccgatcgcgt ccatgtccca ctcgcggcgc tgcgcggtgc gcgccagcac
     4081 cagcacgacg cccaggatca cggcgctgga caggatgccg ccgaccatcg agatggtcat
     4141 cagcttccac aggctgatgg tgtccagccc gaacgcgccg gccacgacac gcgccgcgac
     4201 cgccgccagc gcggagctga acagggtgag cagcgccgag gcctcgacgt tctgcaccgc
     4261 gaaggagcgc cggtcccacg acggggtgaa ctggccggtc atgatcccgg tgcccaggcg
     4321 cgccgccagc gcgccgaaga tcgcgccgcg catgccgatg gcggcgggca ccagcagcag
     4381 caggccgggc agctggccga gcagcccctc catgtgggcg agcacgacgc ccgcgcccat
     4441 gccgacgccc gtggagatcg ccagggcggc gaagccctgg cgcagggtgc ggcgctcctg
     4501 ggcccagtag gccaggacct cgcgcgcggc cttccctcgg atcgccatga cgaggcgaaa
     4561 gcctagactg ggcgggccct cgctcgatga cgcaggagcc ccgcgtgtcc gacctgttca
     4621 ccctcaccga cgaccaccgt gccttccgcg aggtcgtccg caagatggcc gaggagcgca
     4681 tcgcgccgcg cgccgcggag atcgacgaga ccgccgagtt cccccacgac gtgaaggacc
     4741 tgctggagca gcaaggcctc atcggcctgt tcgtcccgga gtcctacggc ggcgccggcg
     4801 ccgacgcgct caccgtggcg atcctggtgg aggagatcgc gcgcgtgtgc gcgtcgtcca
     4861 gcctcatccc gctggtgcag aagctcggct gcatgccgat cgtcctcggc gcgagcgagg
     4921 agcagaagaa ggcctggctg cccaagctcg ccaccggcga ggagctgatc tcctacgcca
     4981 tcagcgaggc cgaggccggc agcgacccgg cgagcatgcg caccaccgcc gtgcgcgacg
     5041 gcgacagcta cgtgctcaac ggcaccaagg tgtggatctc cggcgcgagc gtcgccgact
     5101 gcttcaccgt gctggccaag accgaccccg gctcgggcgc caagggcgtg tcggccttct
     5161 acgtccgcgc cgacgacgcc ggcttcagcg tgggcaagaa ggaggacaag ctcggcatca
     5221 agggctcgcc cacctgccag atccacttcg acaacgtgcg cgtcccggcc gaccgcctga
     5281 tcggcgagga gggcaagggc ttcacctacg cgatgcaggc cttcgaccac acccgcctgg
     5341 cggtgggcgc gcaggccgtc ggcatcgcgc agggcgcgct cgacttcgcc gccggctacg
     5401 tcaaggagcg ccagcagttc ggcaagccca tcggcagctt ccagggactg cagttcatga
     5461 tcgccgacat ggccaccgac atcgacgccg cccgcgcgct ggtctacatc gcggcgggca
     5521 aggccgaccg cggcgacgag gacctgacgc gctggagctc catggccaag ctcaaggccg
     5581 gcgacgtggc gatgcgggtc accgtcgacg cggtgcagct gctgggcggg tacggctaca
     5641 ccaaggacta ccccgtcgag cgctacatgc gcgacgccaa gatcacgcag atctacgagg
     5701 gcacgcagca gatccagcgc atggtgatcg cgcgccaggt cctggggagg ctgtgagccc
     5761 atgagcgacc gccacgccgt gctcggcacg ccgatgcacc cgccgttccc ggaagggctg
     5821 gagcgcgcgg tgttcggcat gggctgtttc tggggcgccg aggagctgta ctggcaggtc
     5881 gacggcgtgt tcaccacggc ggtgggctac gccggcgggg agaccgccgg cccgtcctac
     5941 gagctggtct gcacggggcg caccggccat gccgaggtcg tgttggtcgt gttcgacccg
     6001 gcgcgcgtga gctacgccga actgctgaag atgttctggg agcagcacga cccgacgcag
     6061 ggcatgcgcc agggcaacga catcggcacg cagtaccgct cgctgatcca ggtgcactcg
     6121 gcggagcagc gccgcaccgc cgaggcgtcg cgcgacgcct atcagcaggc gctgaacaag
     6181 gccggccgcg gcgcgatcac cacggagatc accggcgcga cggagttctg gtacgccgag
     6241 ccgtcccacc agcagtacct ggccaagaac ccgcacggct accgctgcca cgcgcgcacc
     6301 gacgtgccgt tcccggcggg cgtcggatcg tgagctcacc gcaggagccg cacagggacg
     6361 ggcgccgtgt cgccgacagc gaggtgacct tcgcccggac catgacgcag ctcgacgcga
     6421 acctggtcgg caaggtgcac ggcggcgtga tcatgcgcga ggtcgactcg gcggccggca
     6481 ccgcggcgtc gcgccacgcc ggccgcgtct gcgtcaccgc cgcgatcgac gagctgagct
     6541 tcctgcagcc ggtggaggtg ggcgacatcc tcatcgtgaa ggcgcgcgtc aactacgtcg
     6601 gccgcacctc catggaggtc ggcgtgcgcg tggaggccga gccgtggacg ggcggcgagc
     6661 ggcgccacac caccagcgcc tacctcacga tggtggcgct gggccaggac ggcaagccgg
     6721 tcgaggtgcc gccgctggtc ccggagaccg acgaggagcg ccgccgctgc gagcgcgccc
     6781 gcatccggcg cgagatgcgc aaggagcgca tcgagcggct gggctgctac ggccccggcc
     6841 ccgtgaccgc ccaggccgac gacacgcccg ccggctgagg cgtggaggac ttcgcgctgg
     6901 cctacggcgt cgcgctcgtc gcggagctgg gcgacaagag ccagctcgcc gccgcgtcgt
     6961 tcggcgcccg tcaccgggtg ctgcccgtgc tcctgggctt cacgctgggc gccgcggcgg
     7021 cgctcggcct gggcgtgctg ctgggcgcgg ccgtcgggga gctgctggcg ccggaggccc
     7081 gctcggtcgt cgcgggcacg ctgttcctgg ccttcgccgc gtggtcgctg cgctccgacg
     7141 accgcgacga gcaggcgagc gcggccgtgc gcgcccgcgt cgtgttcgcc ggggtggcgc
     7201 tggccgtgtt cgtggcggag atgggcgaca agacgcagtt cgccaccgcg gcgctcgcgg
     7261 cgcgccaggc ggcgctgccg acgtggctcg gcgcgacgct cggcgaggtc accgccagcg
     7321 gcgcggccat cctggtgggc gcgtggctcg ggccgcgcat gccggagcgc gcgctgcgca
     7381 tcggcggcgc ggtggtgttc gccgccgtgg ggatcgcgat gctggccgac gggctggtgg
     7441 gctaggtgcg ccgcctgggg ctggtcgcag cgggcggcgc cgccggggcg ttcgtgcgcg
     7501 ccgtcgtggc ggacgtggtc ccgcggaccg gggggctgcc gctggccacg ctgctggtga
     7561 acctggcggg cgcgttcgcg ctcgggctgc tgctcggccg cgtgggcgag gacccgcgtc
     7621 acggcgcgct gccgcgcgcg ctgatcggcg tgggcttcct gggcgccctg acgacgttct
     7681 ccgccctggc cgtggaactc gccctcatgc ccgtgccggc cgccgccggc tacggcctcg
     7741 ccacgctggc cggcgggctg gccctggcgc ggacgggcct ccggctgggg cgtgcccgat
     7801 gacccgcatc caggcgttcc tcgccatcgc cgccgccggc gggctcggcg ccgtcgcgcg
     7861 ggacgccgcc gaccgggcac tgacccgccg gctgcccggc gcgcgcctgc ccgcggggat
     7921 cctggcggtc aacgtcctgg gc
//
LOCUS       DATMKC010000211         5962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_167715, whole genome shotgun sequence.
ACCESSION   DATMKC010000211 DATMKC010000000
VERSION     DATMKC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167715"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2239)
                     /locus_tag="VNU01_06205"
     CDS             complement(<1..2239)
                     /locus_tag="VNU01_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32247.1"
                     /translation="MVFALALQFLAPLQGVSTALVGPVELLLPDPDSNTQSDAQPDEA
                     RGADGIVAPQNHGCGEGGVPTEVASCATDAATCVEEMSEPPADCVTPLVQAVQEATEP
                     GEEPEPPCAEGTPQQQLVCAVDSVRTEACPDDEETCAPLDLVSACLEAPDVPGCAAST
                     VVAETAKAVCDALGDEDPEEGAPTCKERLTDCAQPDSGACEDALEYLAERLETEACDS
                     ASEACVVVAQIETLLACASSQACAFQAVWDEATPLLCELVGDGECQTTLDTCDDPTQG
                     SGCQDAADYLLERSESSACAAEDAVCLAQGALEDCAAVDAQSCESGLYALVVDVACAG
                     TGDCPPEPLPSMRDCVSWADGAPQPNAPCVQAAVVAAICAAQAGSCVLEHPAIQALLV
                     AADDCQGESDEGECERGIQAVAVAALCADPGAEPCFETWAQEAAACKGPSDLACHALA
                     LLHQACGDGGCPAELAALEPCVSGAATQKCGEDDLVPFLRGWIIEQCVAKGSASGACP
                     ERAEVRVPRDLASPPDSQPVREPSVFQNGSTSFPFHTIGAALSALHTPAAWHLALDGR
                     INVGGGTYAETVSIPANTRDITILTPSGVPPTLDGGSGTALSLAKGHRLRITGALHLA
                     GAVPVSGPSVTTSLDIRDRRVYVDRAGSMELSYANVHPTPTQTVATPETVAVVLAISG
                     PSGVTASDVQVQVDGSPVTLTDAGGTFEATVASGIGGIGVEPSDPVTFSGRRTVTLTV
                     AAPGVY"
     gene            complement(2434..4029)
                     /locus_tag="VNU01_06210"
     CDS             complement(2434..4029)
                     /locus_tag="VNU01_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWH32248.1"
                     /translation="MGHGQRAIQGWGFVEAALRPDEVEGIRTLLRERFPQAPLDTRGP
                     KPLAEAGLRRPRARVPDALAPFCATDDEQRALHALGRSFRDMVRLFEGDFSSAPDVVA
                     MPRTEAEAAAVLEWCHAEGLPCVPFGGGTSVVGGVEGPAARGPVVTLQMRSLDRVLEV
                     DRDSRAARIQAGATGPVLEEQLRADGLTMRFFPQSFEMATLGGWIATRAGGHYATKLT
                     HIDDLVESVRALTPAQGVWESRRLPGSGAGPSPDRLLLGSEGILGVITEAWVRLQDRP
                     RWRASASVRFPDFLSGARAVRALAQSGLDPANCRLLDPYEAMLFGGGDGEHALLVLGV
                     ESAHHPVEEDGRLALQLCLDAGGSCPEGLRHREGGQDEGDPASTWRQNFLRAPYLRDA
                     LVAVGMITETFETAVTWDRFAELHDGVKVAVRKALDEVCGGGVVTCRITHAYPDGAAP
                     YFTVIAPSRHGGQVAMWDEVKAAASEALLAAGGTITHHHAVGRDHRPWYDRQRPEPFA
                     AALAGAKAAVDPSGTLNPGVLLG"
     gene            4277..4933
                     /locus_tag="VNU01_06215"
     CDS             4277..4933
                     /locus_tag="VNU01_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH32249.1"
                     /translation="MSVVDDTQEIAAPQDAGMAEAAASSAQASRVVGDPYVVEQVRLA
                     QEGSAHAFALLYDHYVDQIFGYVYHRVGHRQTAEDLVGDVFLRALRRICTFTWQGVDF
                     GAWLMVIARNRVNDHFKSARFRLESTVDEIFDTPATSAQDDPEHQAVTSDLGRTVRTA
                     LAQLKGEQAEVLYLRFVQHLNVAETGAVMGKTEGAVKALQYRALKSLAKLMPDGMVQP
                     "
     gene            4979..>5962
                     /locus_tag="VNU01_06220"
     CDS             4979..>5962
                     /locus_tag="VNU01_06220"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HWH32250.1"
                     /translation="MRNREAEALARLLEDGPARSGEGSAELRRLSALAETLVSAAPGG
                     GAPDTAFKTELRAMLVEEARLQQAQAAPRILPRVRAATATAVERWRYSTRVAAASLAA
                     ALTFSGGGAVAVAAERALPSDALYGVKLVLDDARAAVRFDSVAKGEIHLANAAERIDE
                     AEQSAALGDESGAARALHESADSARKGARELIRGYEERGDAQIVARLSDFAEAQSRRV
                     RVLAEQLSGEGRQAARDSLVVLERIEARLVAIGGVCGDCPAEAPLSGGAFDFSEIPPA
                     DEPFQPCPCDDGDGDGDGAVASDRDGDTDDGDTDDGDATAPPPGDPPPPDED"
BASE COUNT          834 a         2220 c         2118 g          790 t
ORIGIN      
        1 ggtacacacc cggcgccgca acggtcaacg tgacggtcct gcgtccagag aaggtgaccg
       61 ggtcgctggg ctcgacgccg atgccgccga tgcctgacgc gaccgtggcc tcgaacgtcc
      121 cgccggcgtc ggtgagcgtg actggcgatc cgtccacctg gacctgcaca tcagaggccg
      181 tgacgccaga ggggccactg atggcgagca cgacggcaac cgtctcgggg gtcgccaccg
      241 tctgggtcgg cgtggggtgg acgttcgcgt atgacagttc cattgagccc gcgcggtcca
      301 cgtagacacg ccggtcacgg atgtccaggg acgtggtcac ggacgggccg ctcaccggca
      361 cggcgcctgc aaggtgcagc gcaccggtga tgcgcagccg gtggcctttc gcgagcgaca
      421 gcgccgtgcc ggagccgcca tccagcgtgg gcgggactcc cgatggcgtg aggatggtga
      481 tgtcccgggt gttggcgggg atggaaacgg tctccgcgta cgtgccgccg cccacattga
      541 tgcgcccgtc gagcgcgagg tgccatgccg cgggagtgtg cagagccgac agcgcagcgc
      601 cgatggtgtg gaaggggaag ctcgtcgagc cgttctggaa cacgctcggc tcccggaccg
      661 gctgactgtc cggcggactc gccagatccc tcggcacgcg cacctcggcc ctttccgggc
      721 aggccccgct tgcggagccc ttggcgacgc actgctcgat gatccagccg cgcaggaacg
      781 ggacgaggtc gtcctcaccg cacttctgtg tcgcggctcc gctcacacac ggctccaacg
      841 ccgcgagttc cgccgggcac ccaccgtccc cgcacgcctg gtgcagcagc gccagtgcgt
      901 ggcaggcgag gtcgctcggg cccttgcagg cggccgcctc ctgagcccag gtctcgaagc
      961 acggctccgc acccgggtcc gcgcaaagcg ccgccaccgc gacagcctga atacccctct
     1021 cgcactcgcc ctcgtccgat tcgccttggc agtcgtccgc ggcgacgagc aacgcctgga
     1081 tagccgggtg ctccaggaca catgagccag cttgagcggc gcagatggcg gcgacgacgg
     1141 cggcctgcac gcacggagca ttcggttgcg gtgccccgtc tgcccaggag acgcagtcgc
     1201 gcatcgacgg cagcggctcg ggcggacagt cccccgtccc ggcgcaggcg acgtcgacca
     1261 ccaaggcgta cagcccgctc tcgcatgact gggcgtcgac ggcggcgcag tcctcaaggg
     1321 cgccctgcgc gagacagacc gcgtcctcgg ccgcgcacgc cgacgactcg gagcgctcaa
     1381 gcaggtagtc ggccgcatcc tggcagccac tcccctgcgt gggatcgtcg caggtgtcca
     1441 gtgtggtctg gcactctccg tcgccgacca gttcgcacag cagcggcgtc gcctcgtccc
     1501 acaccgcctg gaaggcgcac gcctgtgacg acgcgcacgc cagcagggtc tcgatctgcg
     1561 cgaccaccac gcaggcctcc gaggccgagt cacacgcttc tgtctccagt cgctcggcga
     1621 ggtactcgag tgcgtcctcg cacgcaccgg aatcgggctg tgcgcagtcg gtcaggcgct
     1681 ccttgcacgt cggcgcgccc tcttccgggt cctcatcacc gagcgcgtcg cagaccgcct
     1741 tggccgtctc ggccacgact gtcgaagcgg cgcagcccgg cacgtccggt gcctccaagc
     1801 acgccgacac caggtccagt ggcgcacacg tctcctcgtc gtctgggcac gcctcggtgc
     1861 gaaccgagtc caccgcgcag accagttgct gctgcggcgt cccctccgcg cacggcggct
     1921 cgggctcctc gcccggttcc gtcgcctcct gcactgcctg gaccagcggc gtcacgcagt
     1981 cggccggggg ctcggacatt tcctcgacgc aggtcgcggc gtcggtggcg caactggcga
     2041 cttcggtggg aacgccgcct tcaccacacc cgtggttctg tggagccacg atcccgtcgg
     2101 cgccgcgcgc ctcatccggc tgagcgtccg actgggtatt cgagtcagga tccggcagga
     2161 gcagttcgac cgggccgacg agggcggtcg agacgccctg gagcggcgca aggaactgca
     2221 gggcgagcgc gaacaccacc gggatcgtcc atctgcgtcg gatccgtgtc cccatgtctc
     2281 gttcccgctt tcgtccgtgg cccccggact cacgcgacgc cccgccacac ggccccggaa
     2341 ggccgcgcgt cactggttct tacggtggag cgcggcggaa gttacggaat cggcacacat
     2401 tcggcatggg gcatcgccgg ataatcggac gtatcacccc aaaaggacgc cgggatttaa
     2461 agttccggat gggtctacgg cggccttggc cccggcgagc gcagccgcga agggctccgg
     2521 ccgctggcgg tcgtaccacg gccgatgatc gcgcccgacc gcgtggtggt gcgtgatcgt
     2581 gccgcccgcc gccagcagcg cctcgctcgc ggcggccttc acctcgtccc acatggcgac
     2641 ctggccgccg tggcggctcg gcgcgatgac ggtgaagtac ggggcggctc catccggata
     2701 ggcgtgggtg atgcggcagg tcaccacgcc gccgccgcac acctcgtcga gcgccttgcg
     2761 caccgcgacc ttgaccccgt cgtgcagctc ggcgaagcgg tcccaggtca ccgcggtctc
     2821 gaaggtctcc gtgatcatgc ccaccgccac cagcgcgtcg cgcaggtagg gggcgcgcag
     2881 gaagttctgc cgccaggtgg acgcgggatc gccctcgtcc tggccgccct cccggtggcg
     2941 cagcccctcc gggcaggagc cgcccgcgtc aaggcagagc tgcagcgcca gccggccgtc
     3001 ctcctccacg ggatggtgcg ccgactccac gcccagcacc agcagggcgt gctcgccgtc
     3061 cccgccgccg aacagcatcg cctcgtaggg atcgagcagg cggcagttcg ccgggtccag
     3121 gccggactgc gccagcgcgc gcaccgcgcg ggcccctgac aggaagtccg ggaagcgcac
     3181 ggaggccgac gcgcgccagc gcgggcggtc ctgcaggcgc acccacgcct cggtgatcac
     3241 gccgaggatg ccctcggaac ccagcagcag gcggtcaggc gacggccccg cgccggagcc
     3301 gggcaggcgc ctggactccc acacgccttg cgcgggcgtc agcgcgcgga cggactccac
     3361 caggtcgtcg atatgggtca gtttggtcgc gtaatgcccg ccggcgcgcg tggcgatcca
     3421 gccaccgagc gtcgccatct cgaacgactg cgggaagaag cgcatggtca gcccgtcggc
     3481 ccgcagctgc tcctccagca ccggcccggt ggcccccgcc tggatgcgtg ccgcgcgcga
     3541 atcgcggtcc acctccagca cccggtccag cgaccgcatc tggagcgtca cgacgggccc
     3601 ccgggcggcc gggccctcca cgccgccgac cacgctggtg cccccgccga acggcacgca
     3661 gggcagcccc tcggcatggc accactccag caccgcggcg gcttccgcct ccgtgcgcgg
     3721 catggcgacc acgtccggcg ccgaggagaa gtcgccctcg aacagcctga ccatgtcgcg
     3781 gaaggaccgg ccgagcgcgt gcagcgcacg ctgttcgtcg tccgtcgcgc agaacggcgc
     3841 cagggcatcg ggcacgcgcg cgcgcggccg gcgcaggccg gcctccgcga gcggcttggg
     3901 gccgcgcgtg tccagcggcg cctgcgggaa acgctcgcgc agcagcgtgc ggatgccctc
     3961 cacctcgtcc gggcgcagcg ccgcctcgac gaagccccag ccctggatcg cccgctgccc
     4021 gtggcccatt tccttgcctt tcgccgcttc agcccctcat cgcgcgtgcc gaacgctacg
     4081 gtatggagtg gtccaccgcc ccccggtcgc gcgcgcgccc gggaggcccc gcgcagcgcc
     4141 ggcccgcctc gggggggccg gcatcggagt gcactccaga tgaggccagc aaccaccatc
     4201 gccctcgcgg tgctgctgct cgccatcgtg gcggccatgg tcgtccagtt gctgctggcg
     4261 ccgggctagg cgccgcatga gcgtcgtgga cgacacccag gagatcgccg cgccccagga
     4321 cgcgggcatg gccgaggcag ccgcctccag cgcccaggcc tcgcgcgtcg tgggtgaccc
     4381 gtacgtggtc gagcaggtcc ggctggcgca ggagggctcg gcgcacgcgt tcgccctgct
     4441 gtacgaccac tacgtcgacc agatcttcgg ctacgtctac caccgcgtcg gccaccgcca
     4501 gaccgccgag gacctggtgg gcgacgtgtt cctgcgcgcg ctgcggcgca tctgcacgtt
     4561 cacctggcag ggcgtggact tcggcgcctg gctgatggtg atcgcgcgca accgcgtcaa
     4621 cgaccacttc aagagcgcgc gcttccggct cgagtccacc gtcgacgaga tcttcgacac
     4681 gccggccacc agcgcccagg acgacccgga gcaccaggcg gtgaccagcg acctgggacg
     4741 cacggtcagg acggcgctcg cccaactgaa gggcgagcag gccgaagtgc tctacctgcg
     4801 cttcgtgcag cacctcaacg tcgcggagac cggtgcggtc atgggcaaga cggagggcgc
     4861 ggtcaaggcc ttgcagtacc gggcactgaa gtcgctggcg aagctcatgc cggacgggat
     4921 ggtgcagcca tgagcgcgtg cctttttgcg gcctgcgtcg ttaaggcggg tgagacctat
     4981 gcgcaatcgt gaggctgagg cgctggcgcg cctgctggaa gacggccccg cccgttcggg
     5041 cgaggggtcg gccgagctgc gccggctgag cgcgctcgcc gagacgctgg tgtccgccgc
     5101 gcctggcggc ggcgcgcccg acaccgcgtt caagacggag ctgcgggcca tgctggtcga
     5161 ggaggcgcgc ctgcagcagg cacaggccgc gccgcggatc ctgccgcgcg tgcgcgccgc
     5221 gaccgccacc gccgtggagc gctggcgcta ctccacccgt gtggcggcgg cgtcgctggc
     5281 cgccgcgctg acgttctccg gcggcggggc cgtcgccgtg gccgccgagc gcgcgcttcc
     5341 cagcgacgcg ctgtacgggg tgaagctggt cctggacgac gctcgcgcgg cggtgcgctt
     5401 cgacagtgtc gccaagggcg agatccacct ggccaacgcg gcggagcgca tcgacgaggc
     5461 ggagcagtcc gccgcgctgg gagacgagtc cggggcggcg cgcgccctgc acgagtccgc
     5521 cgacagcgcc cgcaagggcg cgcgggagct catccggggc tacgaggagc gcggcgacgc
     5581 ccagatcgtg gcgcggctca gcgacttcgc ggaggcccag agccgtcgcg tccgcgtgct
     5641 ggccgagcag ttgtccggcg agggccggca agcggcccgc gactcgctgg tggtgctgga
     5701 gcgcatcgag gcgcggctgg tcgcgatcgg cggcgtgtgc ggggactgcc ccgccgaggc
     5761 gcccctgagc ggcggcgcgt tcgacttctc cgagatcccg ccggcggacg agccgttcca
     5821 gccgtgcccc tgcgacgacg gcgacggcga cggcgacggc gccgtggcct ccgaccgcga
     5881 cggcgacacc gacgacggcg acaccgacga cggcgacgcg acggcgcccc cgccgggcga
     5941 cccgccaccg cccgacgagg ac
//
LOCUS       DATMKC010000212         5763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_120663, whole genome shotgun sequence.
ACCESSION   DATMKC010000212 DATMKC010000000
VERSION     DATMKC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120663"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..2875
                     /gene="alaS"
                     /locus_tag="VNU01_06225"
     CDS             200..2875
                     /gene="alaS"
                     /locus_tag="VNU01_06225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWH32251.1"
                     /translation="MQSSDIRRTFLDHFRAQDHRVWPSSSLIPNDPTLLLTVAGMVQF
                     KPFFLGESLPEHPRAASVQKCARTLDIENVGVTTRHLTFFEMLGNFSFGDYFKREAIR
                     WAWELSTRPAAPGEGGGFGFDPERIWATVYTDDDEAERLWLEESGIPASRIVRRGKKD
                     NFWSTGGAGPCGPCSELYYDRGPEHGAEGGPEADENRYLEFWNLVFMQFEQDAAGNIV
                     GTLPKQNVDTGMGLERMAVLLQDVPNVYEIDTLGTLLSRALELTNFDEGQYQRGGPSA
                     VSARVLAEHSRSAAMLIADGVLPSNEGRGYILRRLLRRAVRHVRLLGEDKPVMPDMMH
                     TVIEVMGDAWPELKEQSDLILKVAQSEEQSFSRTLSAGMSILEKEIGHANDLSGAQML
                     SGETAFKLHDTYGFPIELTLEIAQERGLLLDRDEFLRAMEEQRTRARGARRGKAEGTP
                     VEVYREAAAAVGGVEFLGYDKESAESKLAVLLTADGPTERAGEGDELEVVLPLTPFYA
                     EGGGQLGDHGVIQTETGRLEVLDTVAPISGLTVHRARVVAGEIAAGQPAHAEIDHARR
                     ASIRRGHTATHILHASLKEVVGDHAAQAGSAIDAGRFRFDFPHFSAVEREQLAAVEAL
                     VNDRIAAGPEVRTTETTLDRAREMGATALFGEKYGERVRVVEIGEYSRELCGGTHVGH
                     ATEVGLFTIVSEGSIAANLRRIEAITGPEAFAYLSKERLVAEQVARLLKVSGDEVAER
                     VGDLLERLRAAEKEIARLRQEALMGRAAALLGGAEDVDGARLIAAEVPGADRDGLKAL
                     ASDLANRAGTGVVVLGARTDDGSALLFAAVSGDLDAEGVGARDVLQPGAKAVGGGAGG
                     KGATAQAGGKDGGKLADALEASRVAAREAIRAARA"
     gene            2889..3335
                     /gene="ruvX"
                     /locus_tag="VNU01_06230"
     CDS             2889..3335
                     /gene="ruvX"
                     /locus_tag="VNU01_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWH32252.1"
                     /translation="MRVLGIDAGEVRIGVALSDPDGVIASPLATVPAGGDAAERLAGL
                     AREHGCALAVVGLPKGMSGRDTASTQMARRLAKSLGELGLDVTLWDERLSSAQAERVL
                     LGAGRRRDQRRAERDRVAAAIILQSWLDAHGGAGGAAGPSGTEEPS"
     gene            3332..4324
                     /gene="mltG"
                     /locus_tag="VNU01_06235"
     CDS             3332..4324
                     /gene="mltG"
                     /locus_tag="VNU01_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWH32253.1"
                     /translation="MTKLLTAVVAAALLAAAGFTYLSGGTEGGEPVAFTVPEGVGASA
                     LADDLAEAGVIRSALAFKLRARLDDRAAAILAGEYELRTGMGVSEVLDALTAGPVEPD
                     TFRVLIREGLTVKQTLEAIAGSEHSPFTVEELEAALDGVALPDWVPLEELPGAAEPFE
                     GLLFPDTYDFPLDIDPQSLLSRLVLQTERVMEGIQPPPNLSRYQVLVMASLVEREARL
                     REEQPRIAAVMHNRLARDMALQIDATVLYALGEHKDRVLYSDLEIDSPWNTYRSKGLP
                     PTPISGAGRAAIEAAAKPAQEDYLYYVVVDPQTGRHGFSRTLEEHNRLRQEARS"
     gene            4392..5213
                     /gene="aroE"
                     /locus_tag="VNU01_06240"
     CDS             4392..5213
                     /gene="aroE"
                     /locus_tag="VNU01_06240"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWH32254.1"
                     /translation="MRRVRLFGHPVAHSVSPAIHNAAFAALGIEAVYEALDVAPEALP
                     AAVAELRATPLLGANCTIPHKLAVLDLVDERTEETRLIGAANTLFWRDGALAADNTDA
                     RGLEQAIRAVWTPEPGGKAVLLGSGGAARAAAVALARLGLAVAVVARRPVPAESVAAT
                     AAEAGAEGVGLPLEHARAALDDAVLVVNATPLGLHGEPLPEPFMRLRPEQFALDLVYG
                     GGDTPFLAAARAAGCPTEDGLGMLVGQAALSFERWTGRQAPLDVMRQAAEAALGR"
     gene            5394..>5763
                     /locus_tag="VNU01_06245"
     CDS             5394..>5763
                     /locus_tag="VNU01_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32255.1"
                     /translation="MVVIAVVFSGLAQLLVSSFRSVAVSEERLRATQIASEAIEELQA
                     LEWDRIGFYSSQNPPTPVVDGHSNWTVVNLGTTLPTTRSASRRPLAAPSTPWSSRWRC
                     RATSPRTPPRSPTVSAPGRAS"
BASE COUNT          778 a         2032 c         2205 g          748 t
ORIGIN      
        1 ccccgaggcc gtgctcggcg cgcagctccg cctccttggc ggccatcgcg gcgctggagg
       61 ccggccgcgc cgcgatggcc gccaaggagg cggagctgcg cgccgagcac ggcctcgggg
      121 gcggcggtgc gacgccgtac cctggcggca gccagttccc gccagactga ccgctccgga
      181 aagcaaggtc accgccgaca tgcagtcctc cgacatccgc cggaccttcc tggaccattt
      241 ccgggcccag gaccaccgcg tgtggccgtc gtcgtcgctg atccccaacg acccgacgct
      301 cctgctcacc gtggccggga tggtgcagtt caagccgttc ttcctgggcg agtccctgcc
      361 ggagcacccg cgcgcggcga gcgtgcagaa gtgcgcgcgc acgctggaca tcgagaacgt
      421 gggcgtgacc acccggcacc tgacgttctt cgagatgctg ggcaacttca gcttcggcga
      481 ctacttcaag cgcgaggcga tccgctgggc ctgggagctg tccacgcgtc cggccgcgcc
      541 gggcgagggc ggcggcttcg gcttcgaccc cgagcgcatc tgggcgaccg tctacaccga
      601 cgacgacgag gccgagcggc tctggctgga ggagtccggc atccccgcgt cccgcatcgt
      661 gcgccgcggc aagaaggaca acttctggtc caccggcggc gccgggccgt gcgggccgtg
      721 cagcgagctg tactacgacc gcggaccgga gcacggcgcc gagggcggcc cggaggcgga
      781 cgagaaccgc tacctggagt tctggaacct ggtgttcatg cagttcgagc aggacgccgc
      841 gggcaacatc gtcggcacgc tgccgaagca gaacgtcgac accggcatgg gcctggagcg
      901 catggccgtg ctgctgcagg acgtgcccaa cgtctacgag atcgacacgc tcggcacgct
      961 gctgagccgc gcgttggagc tcaccaactt cgacgagggt cagtaccagc gcggcggccc
     1021 tagcgccgtc agcgcccggg tgctcgcgga gcattcgcgc agcgccgcca tgctgatcgc
     1081 cgacggcgtg ctccccagca atgaggggcg gggttacatc ctgaggcggc tgctccgccg
     1141 ggctgtgcgg cacgtgcgtt tgctgggcga ggacaagcca gtcatgcccg acatgatgca
     1201 taccgtcatc gaggtcatgg gcgatgcgtg gcctgagctg aaggaacaat ctgatctgat
     1261 tctcaaggtc gcgcaatcgg aggagcaatc gttttcgcgc acgctctcag cagggatgtc
     1321 gattctcgaa aaggagatcg ggcacgcgaa tgacctctcg ggcgcccaga tgttgtccgg
     1381 ggaaactgcg ttcaagttgc acgacacgta cggattccct atcgagttga cgctcgagat
     1441 cgcgcaagag cgcgggctgc tgctcgacag ggacgagttt ctccgcgcga tggaggagca
     1501 gcgcacgcgt gcccgcggcg cccggcgcgg caaggccgag ggcacccccg tggaggtcta
     1561 ccgggaggcc gcggcggcgg tcggcggcgt ggagttcctg ggctacgaca aggagtccgc
     1621 cgagagcaag ctggccgtgc tgctcaccgc cgacgggccc acggagcgcg ccggggaggg
     1681 cgacgagctg gaggtcgtgc tcccgctgac ccccttctac gccgagggcg gcgggcagct
     1741 cggcgaccac ggcgtgatcc agacggagac cgggcgcctg gaggtgctgg acaccgtcgc
     1801 gcccatcagt gggctgaccg tgcaccgcgc gcgggtggtg gccggcgaga tcgccgcggg
     1861 ccagcccgcc cacgccgaga tcgaccacgc gcggcgtgcc tccatccgcc ggggccacac
     1921 cgccacgcac atcctgcacg cctccctgaa ggaggtcgtc ggcgaccacg ccgcccaggc
     1981 cggctcggcc atcgacgcgg ggcgcttccg cttcgacttc ccgcacttct ccgcggtgga
     2041 gcgcgagcag ctcgcggccg tcgaggcgct ggtgaacgac cgcatcgccg cgggccccga
     2101 ggtgcggacc acggagacga cgctcgaccg ggcgcgcgag atgggcgcca cggcgctgtt
     2161 cggcgagaag tacggcgagc gcgtgcgcgt ggtcgagatc ggcgagtact cccgcgagct
     2221 gtgcggcggc acgcacgtgg gccacgccac ggaggtcggc ctgttcacca tcgtgtcgga
     2281 ggggtcgatc gcggcgaacc tgcggcgcat cgaggcgatc accgggccgg aggcgttcgc
     2341 gtacctgtcc aaggagcgcc tcgtcgccga gcaggtcgcg cgcctgctca aggtctccgg
     2401 ggacgaggtg gccgaacgcg tcggcgacct gctggagcgc ctgcgcgcgg cggagaagga
     2461 gatcgcccgg ctgcgccagg aggcgctcat gggccgcgcc gccgcgctgc tgggcggtgc
     2521 cgaggacgtc gacggcgcgc ggctgatcgc cgcggaggtg cccggcgcgg accgcgacgg
     2581 cctgaaggcg ctggcgagcg acctcgccaa ccgggccggg accggcgtgg tcgtgctggg
     2641 cgcccgcacc gacgacggca gcgccctgct gttcgccgcg gtctcggggg acttggacgc
     2701 cgagggcgtc ggcgcgcgcg acgtgctgca gcccggcgcc aaggcggtcg gcggcggcgc
     2761 cggcggcaag ggcgcgaccg cgcaggcggg cggcaaggac ggcggcaagc tggccgacgc
     2821 gctggaggcg tcccgcgtgg cggcgcgcga ggccatccgc gcggcgcggg cctgacgcgg
     2881 agggcggcgt gcgcgtgctg ggcatcgacg cgggcgaggt gcgcatcggc gtggccctca
     2941 gcgaccccga cggcgtgatc gcctcgccgc tggccaccgt gccggccggc ggcgacgccg
     3001 ccgagcggct ggcgggcctg gcgcgcgagc acggctgcgc gctggcggtg gtgggcctgc
     3061 ccaagggcat gtccgggcgc gacaccgcct cgacccagat ggcgcggcgg ctggcgaagt
     3121 ccctggggga gctgggcctg gacgtgacgc tgtgggacga gcgcctcagc tccgcgcagg
     3181 ccgagcgcgt gctgctcggg gccggtcgcc ggcgcgacca gcggcgggcg gagcgcgacc
     3241 gcgtggccgc cgcgatcatc ctgcagagct ggctggacgc ccacggcggc gccggcgggg
     3301 ccgcgggccc gtccggcacg gaggagccat catgacgaag ctgttgaccg cggtggtcgc
     3361 cgcggcgctc ctggccgccg ccgggttcac gtacctgtcg ggcggcaccg agggcggtga
     3421 gccggtcgcg ttcaccgtgc ccgagggcgt gggcgcgtcg gcgctcgcgg acgacctggc
     3481 ggaggccggc gtgatccgct cggcgctggc gttcaagctg cgggccaggc tggacgaccg
     3541 cgccgcggcc atcctggccg gcgagtacga gctgcgcacc gggatgggcg tctccgaggt
     3601 gctggacgcg ctgaccgcgg gcccggtcga gccggacacg ttccgggtgc tcatccgcga
     3661 ggggctgacc gtcaagcaga cgctggaggc catcgcgggc tcggagcaca gcccgttcac
     3721 cgtggaggag ctcgaggccg ccctggacgg cgtcgcgctg ccggactggg tgccgctgga
     3781 ggagctgccg ggcgccgccg agccgttcga ggggctgctg ttccccgaca cctacgactt
     3841 cccgctcgac atcgacccgc agtcgctgct gtcgcgcctg gtgctgcaga cggagcgcgt
     3901 catggagggc atccagccgc cgccgaacct gtcgcgctac caggtgctgg tgatggcgtc
     3961 gctggtcgag cgcgaggcgc gcctgcgcga ggagcagccg cgcatcgccg cggtgatgca
     4021 caaccgcctg gcccgcgaca tggcgctgca gatcgacgcg acggtgctgt acgcgctggg
     4081 ggagcacaag gaccgggtgc tgtactcgga cctggagatc gactcgccct ggaacaccta
     4141 ccgctccaag gggctgccgc cgacccccat ctccggcgcc ggccgcgcgg cgatcgaggc
     4201 ggccgccaag ccggcccagg aggactacct gtactacgtc gtggtggacc cgcagaccgg
     4261 gcgccacggc ttctcgcgca ccctggagga gcacaaccgc ctgcgccagg aagcccgctc
     4321 gtgacccgcg agtgggtgcc cccgttgcgg tatcacgcgg tgcccgcacc cgttcgaacg
     4381 ggtgcgcggg gatgaggcgc gtgcggctgt tcggccaccc ggtcgcgcac agcgtgtccc
     4441 cggcgatcca caacgccgcg ttcgccgcgc tgggcatcga ggcggtctac gaggcgctcg
     4501 acgtggcgcc ggaggcgctg cccgcggcgg tggccgagct gcgggcgacg cccctgctcg
     4561 gggcgaactg caccattccc cacaagctgg ccgtgctcga cctggtggac gagcgcaccg
     4621 aggagacgcg gctgatcggc gcggcgaaca cgctgttctg gcgcgatggc gcgctggccg
     4681 ccgacaacac cgacgcgcgc ggcctggagc aggcgatccg ggcggtgtgg acgccggagc
     4741 cgggcggcaa ggcggtcctg ctgggctccg gcggcgccgc ccgcgcggcc gccgtggcgc
     4801 tggcgcgcct gggcctggcc gtggccgtgg tggcccggcg ccccgtgccc gccgagtcgg
     4861 tggcggccac ggccgccgag gccggcgcgg agggggtcgg cctgccgctg gagcacgcgc
     4921 gggcggccct ggacgacgcc gtgctcgtgg tcaacgccac gccgctcggg ctgcacggcg
     4981 agccgctgcc ggagccgttc atgcgcctgc gccccgaaca gttcgccctg gacctggtgt
     5041 acggcggcgg cgacacgccg ttcctggcgg cggcgcgcgc cgccggctgc cccaccgagg
     5101 acggcctggg catgctggtg ggccaggcgg cgctgtcgtt cgagcgctgg accggccggc
     5161 aggcgccgct ggacgtgatg cggcaggccg ccgaggccgc gctcggccgg tagcccggcc
     5221 gcagcaaatc cgcatgaacc ggacactcaa gtccgagact gcttctggtc gacatagctg
     5281 gagaccgatt tctggccagg agcaggtcaa ctgtgctgac accaccgggc gggcggcctc
     5341 gcaggcgcct gcatgacgac agcggcctcg ggctcgtcga ggtcctcgtc gccatggtcg
     5401 tcatcgcggt ggtgttctcc gggctcgcgc agctcttggt gagctcgttc cgcagcgtcg
     5461 cggtgtccga ggagcgcctg cgcgccaccc agatcgcctc ggaggcgatc gaggagctgc
     5521 aggcgctgga gtgggaccgc atcgggttct acagctccca gaacccgcct acgcccgtgg
     5581 tcgacgggca ctccaactgg acggtcgtca acctgggcac gaccctgccg acgacgagat
     5641 ctgcgtcaag acgtccactc gccgcacctt caacaccttg gtcttccagg tggaggtgca
     5701 gggctacctc gcctcggaca cctccgcgct cgccaacggt gtcggcgcct ggacgggcct
     5761 cac
//
LOCUS       DATMKC010000213         5871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_179799, whole genome shotgun sequence.
ACCESSION   DATMKC010000213 DATMKC010000000
VERSION     DATMKC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179799"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VNU01_06250"
     CDS             complement(<1..1728)
                     /locus_tag="VNU01_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWH32256.1"
                     /translation="MPPARSSRSADPLEAFSPATRAWFRASFPQPTPVQAETWRAIHD
                     GGSVLVSAPTGSGKTLAAFLTAIDRLLTTPQPQAAGVRVLYVSPLKALAHDIERNLTA
                     PLVGIRHAAQRLGVDVGAIRVGMRTGDTPPDERRRQAKHPPDILITTPESLYLLLTSA
                     ARSGLAAIDTVIVDEVHAVAGTKRGAHLALTLERLEALRSAADAGAPPLRRVGLSATQ
                     RPLEEIARWLGGGEPPGDSSGDGDAAWTPRPVRIVAPPSDKRLDVEIVVPVEDMSRLG
                     EAAELPASGPAAGPAERNSIWPAIHPRILELVRAHRSTIVFANSRRLAERLTARLNEL
                     AEQEADPDQPFTPIARAHHGSVSREQRLQIEGELKAGRLPCVVATSSLELGIDMGAVD
                     LVIQVESPGAVSRGLQRIGRAGHQVGEPSRGVVFPKYRGDLIECAVVVRRMLDGQIEQ
                     TRYPRNPLDVLAQQIVAMASIQDVRVADVHDIVRRAAPFAELSREQLDGVLDLLSGRY
                     PSDEFAELRPRLVWDRVEDRLSGRPGAQRLAVTSGGTIPDRGLYGVFLAGEGAGRRVG
                     ELDEEMVYEL"
     gene            complement(1796..1999)
                     /locus_tag="VNU01_06255"
     CDS             complement(1796..1999)
                     /locus_tag="VNU01_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32257.1"
                     /translation="MNLDAYYRGLAAEELQRLGDQLMVLSGEAAQAGAHDAAWHLSEL
                     ATELLEMGLDVGRQRTPRAGEPI"
     gene            2130..2540
                     /locus_tag="VNU01_06260"
     CDS             2130..2540
                     /locus_tag="VNU01_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32258.1"
                     /translation="MNRETVMVRIPPTPEHLALLRTAVAGFAARDHFTLDQVDDLRLA
                     VEEGAVQLLKHVTGEGLTLTVSRTEPGIEVRLSADVAGDDPVIDEESFSWVILRALAD
                     DVAIEMQQGHAAIVLAKHRLATAPQLDQDDQDDA"
     gene            2537..3295
                     /locus_tag="VNU01_06265"
     CDS             2537..3295
                     /locus_tag="VNU01_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HWH32259.1"
                     /translation="MTSQYSRDDTRHLFEELRRTGDPTLREELAKLHLPLVEYLAKRF
                     KDRGEPLDDLVQVGSVGLLKAIDRFDLERGVEFSTYATPTIVGELKRYFRDKGWAVRV
                     PRRLQELSLRLNKVVAQLTQDLGRSPTVPEIAKFAGLTEDEVLEALESGQAYSTTSLD
                     APSGEDDDAPLRLDRIGEEDLRIDNLEYFASLAPLIEKLPEREKTILYLRFFKGMTQS
                     TIAERVGISQMHVSRLLTRILEFLREGMEGDSEY"
     gene            3370..4278
                     /locus_tag="VNU01_06270"
     CDS             3370..4278
                     /locus_tag="VNU01_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWH32260.1"
                     /translation="MRALLVHNPNATTTTPAVTDVIAKALSAQLKLDVEATKRRNHAS
                     YLAAGAVHEGYEVVIALGGDGTANEVLQGIAGTDVKLAVIPGGSTNVLARSLGLPNDP
                     VEATSAVLRKLADHDERRIGLGKANGRWFAFNAGFGFDAEVVRFVEQRHRMKRTVRQA
                     TFVYCGMLALASGFGRRPVEVTVTPDDGETVDGLRSAVCCNADPFTYLGRWPARLCPE
                     ADFDKGLDATGLSKVTFLPLLHLAQTALTSPNVHHRKIARLWHDRPGFTFTSSEPLPL
                     QLDGDYVGRTSRVRLESVPGAVTVVA"
     gene            complement(4283..4993)
                     /locus_tag="VNU01_06275"
     CDS             complement(4283..4993)
                     /locus_tag="VNU01_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWH32261.1"
                     /translation="MFTFSPRPPAIVGHRGAPRMAPENSLASFRAAVAAGASWVELDA
                     RRTADDRIAVIHDPATVDGVAVVERRLDELRPHGVVGLEDVFADLPAGVGADVELKNL
                     PGEPDYDEEQRLASLVARVVAAHGGGRPLVASSFNPLALGALRQERADLPLGLLTVPV
                     LELSSGVELAVEFGVDAVFPHVTAPDLTPAAVAAAHARGLAVMVWTVDEPAQARRLAE
                     AGVDALCTNEPALLAAAL"
     gene            5359..5610
                     /locus_tag="VNU01_06280"
     CDS             5359..5610
                     /locus_tag="VNU01_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWH32262.1"
                     /translation="MDWRQKAACLDEDPELFFPVGTTGPALEQVERAKAVCARCEVVQ
                     DCLEWALDSNQDAGVWGGMSEDERRTLRRSRQRRRRMAS"
BASE COUNT          840 a         2201 c         2084 g          746 t
ORIGIN      
        1 cagctcgtag accatctcct cgtccagctc gccgacgcgc cgcccggccc cctcccccgc
       61 caggaacacc ccgtagaggc cgcggtccgg gatggtgccg ccggaggtca ccgccaggcg
      121 ctgggcgccg ggcctgccgg agaggcggtc ctcgacgcgg tcccacacca gccgcgggcg
      181 cagctcggcg aactcgtcgg acgggtagcg gccgctgagc aggtcgagca cgccgtcgag
      241 ctgctcccgc gacagctccg cgaacggcgc ggcgcgccgc acgatgtcgt gcacgtccgc
      301 cacgcgcacg tcctggatgc tcgccatggc cacgatctgc tgcgccagca cgtccagcgg
      361 gttgcgggga tagcgcgtct gctcgatctg gccgtcgagc atgcgccgca ccacgaccgc
      421 gcactcgatc aggtcgcccc ggtacttggg gaacaccacc ccgcggctcg gctcgcccac
      481 ctgatgcccg gcgcggccga tgcgctgcag gccgcgggag accgcgcccg gcgactccac
      541 ctggatcacc aggtccaccg cacccatgtc gatgccgagc tccagcgacg acgtcgcgac
      601 cacgcacggc aggcgccccg ccttcagctc gccttcgatc tgcaggcgct gctcccgcga
      661 caccgagccg tggtgggcgc gcgcgatcgg cgtgaacggc tggtccgggt ccgcctcctg
      721 ctcggcgagc tcgttcaggc gcgcggtgag gcgctcggcc agccggcggg agttggcgaa
      781 cacgatcgtg gagcgatgcg cgcgcaccag ctccaggatg cgcgggtgga tcgccggcca
      841 gatggagttg cgctccgccg gcccggcggc gggcccggac gcgggcagct cggccgcctc
      901 gcccaggcgg gacatgtcct ccaccggcac gacgatctcg acgtcgaggc gcttgtcgga
      961 gggcggcgcg acgatgcgca ccggccgcgg cgtccacgcg gcgtcgccgt ccccagaact
     1021 gtctcctggc ggctcgccgc cgccgagcca gcgggcgatc tcctccaggg gccgctgcgt
     1081 ggcggacagg ccgacacggc gcagcggcgg cgcgccggcg tccgccgccg agcgcagcgc
     1141 ctccagccgt tccagcgtca gcgccaggtg ggcgccgcgc ttggtgccgg cgaccgcgtg
     1201 cacctcgtcg acgatcacgg tgtcgatggc cgccaggccg gagcgcgccg ccgaggtcag
     1261 cagcaggtac agcgactcgg gggtggtgat gaggatgtcc ggcgggtgct tggcctgccg
     1321 ccgccgctcg tccggcggcg tgtcgccggt gcgcatgccg acccggatcg cccccacgtc
     1381 gacgcccagc cgctgcgccg cgtggcggat cccgaccagc ggcgcggtga ggttgcgctc
     1441 gatgtcgtgg gccagcgcct tcagcggcga cacgtacagc acccgcactc ccgcggcttg
     1501 cggctgcggc gtggtcagca ggcggtcgat ggcggtgagg aacgccgcca gcgtcttgcc
     1561 ggagccggtc ggcgcggaca cgagcacgct gccaccatcg tgaattgccc gccaggtttc
     1621 tgcttggacc ggcgtcggct ggggaaagct tgcgcggaac catgcgcggg tggccggaga
     1681 gaacgcctcg aggggatccg cgctccgtga cgatcgggca ggcggcatgg atcccaggat
     1741 agggcgcggg cgtgacagcg cgccgcgaga agtcagccga tactcagatc ggcgctcaga
     1801 tcggctcgcc ggcgcgcggc gtgcgctgcc ggccgacgtc cagccccatc tccagcagtt
     1861 cggtggccag ctcggacagg tgccaggccg cgtcgtgggc gccggcctgg gcggcctcgc
     1921 cggacaggac catgagctga tcgcccagcc gctgcagctc ttcggcggcg agtccgcggt
     1981 agtaggcgtc gaggttcacc acggcgtcgt tcccgttcga tcagcccccc aacccactgc
     2041 ccggcggcaa cgctgagcta ggctcgccag ccggggcccg ccgcacccgg tgcgcagacg
     2101 actcgacgcg agaaggaatg aggagacagg tgaaccgcga aaccgtcatg gtccggatcc
     2161 cgccgacacc cgagcacctg gccctgctgc gcacggcggt ggccggcttc gccgcgcgcg
     2221 accacttcac cctcgaccag gtggacgacc tgcgcctggc cgtggaggag ggcgccgtgc
     2281 agctcctcaa gcacgtgacc ggtgagggcc tcacgctgac ggtcagccgc accgagcccg
     2341 ggatcgaggt ccggctgtcg gccgacgtgg ccggggacga cccggtgatc gacgaggagt
     2401 ccttctcctg ggtgatcctg cgcgcgctgg ccgacgacgt cgccatcgag atgcagcagg
     2461 gccacgccgc catcgtgctc gccaagcacc ggctcgcgac cgccccccag ctcgaccagg
     2521 acgaccagga cgacgcatga cctcgcagta cagccgggac gacacccggc atctgttcga
     2581 agagctgcgc cgcaccggtg atccgacgct gcgcgaggag ctcgccaagc tgcacctgcc
     2641 gctggtggag tacctggcca agcgcttcaa ggaccgcggc gagccgctcg acgacctcgt
     2701 ccaggtcggc tccgtcggcc tgctcaaggc gatcgaccgc ttcgacctgg agcgcggcgt
     2761 ggagttctcc acctacgcca cccccacgat cgtcggcgag ctcaagcgct acttccgcga
     2821 caagggctgg gcggtgcgcg tcccgcgccg cctccaggag ctgtcgctgc ggctcaacaa
     2881 ggtggtcgcg cagctcaccc aggacctggg ccgctccccc accgtgccgg agatcgccaa
     2941 gttcgccggg ctgaccgagg acgaggtgct cgaggcgctg gagtccgggc aggcctactc
     3001 caccacgtcg ctggacgcgc cctcgggcga ggacgacgac gcgcccctgc ggctggaccg
     3061 catcggcgag gaagacctgc gcatcgacaa cctggagtac ttcgcgtcgc tggccccgct
     3121 gatcgagaag ctgcccgagc gcgagaagac catcctgtac ctgcgcttct tcaagggcat
     3181 gacgcagtcg accatcgcgg agcgcgtcgg catcagccag atgcacgtct cgcgcctgct
     3241 cacgcgcatc ctggagttcc tgcgcgaggg catggagggc gactccgagt actgaggccg
     3301 gccggacggc gccggatgga ccggcgccaa ccccgcccgg cttcgggggc ccggtaggat
     3361 tccggcccta tgcgcgcgct gctggtccac aaccccaacg ccaccaccac gacgcctgcc
     3421 gtcaccgacg tgatcgccaa ggcgctgtcg gcccagctca agctggacgt ggaggcgacc
     3481 aagcgccgca accacgcctc ctacctggcc gccggcgcgg tccacgaggg ctacgaggtg
     3541 gtcatcgccc tgggtggcga tggcaccgcg aacgaggtgc tgcagggcat cgcgggcacg
     3601 gacgtgaagc tggcggtgat ccccggcggg tccacgaacg tgctcgcacg cagcctcggc
     3661 ctgcccaacg accccgtgga ggccacgtcc gcggtgctgc gcaagctcgc cgaccacgac
     3721 gagcgccgga tcggcctggg caaggccaac ggccgctggt tcgcgttcaa cgccgggttc
     3781 ggcttcgacg cggaagtcgt gcgcttcgtg gagcagcgcc accgcatgaa gcgcacggtg
     3841 cggcaggcga cgttcgtgta ctgcggcatg ctcgccctgg cctcgggctt cggccggcgg
     3901 ccggtggagg tcacggtcac gcccgacgac ggcgagacgg tggacggcct gcgctccgcg
     3961 gtgtgctgca acgccgaccc gttcacctac ctgggccgct ggcccgcccg gctgtgcccc
     4021 gaggccgact tcgacaaggg cctggacgcc accggcctga gcaaggtgac cttcctgccg
     4081 ctgctgcacc tggcgcagac ggcgctcacg tcgcccaacg tgcaccaccg caagatcgcg
     4141 cggctgtggc acgaccggcc gggcttcacg ttcaccagct ccgagccgct gccgctgcaa
     4201 ctggacggcg actacgtcgg gcgcacgtcc cgcgtgcggc tggagtcggt gcccggcgcg
     4261 gtcaccgtgg tcgcctgacg cgtcacagcg cggcggccag gagcgccggc tcgttcgtgc
     4321 acagcgcgtc caccccggcc tcggccagcc gccgggcctg cgcgggctcg tccacggtcc
     4381 agaccatcac cgccaggccg cgcgcgtgcg cggcggccac ggcggcgggc gtcaggtccg
     4441 gcgcggtcac gtgcgggaac accgcgtcga cgccgaactc cacggccagc tccacgccgc
     4501 tggacagctc cagcaccggc acggtgagca gccccagcgg caggtccgcg cgctcctggc
     4561 gcagcgcgcc cagcgcgagc gggttgaacg agctggccac cagcgggcgg ccgccgccgt
     4621 gggccgccac cacccgggcg accaggctcg ccaggcgctg ttcctcgtcg tagtccggct
     4681 ccccgggaag gttcttgagc tccacgtccg cacccacccc ggcgggcagg tccgcgaaga
     4741 cgtcctccag ccccaccacg ccgtgtggcc gcagctcgtc cagccgccgc tcgacgacgg
     4801 cgacgccgtc caccgtggcc gggtcgtgga tcaccgcgat gcggtcgtcg gcggtccggc
     4861 gcgcgtccag ttccacccag gacgcccccg ccgccactgc ggcgcggaac gacgcgaggc
     4921 tgttctccgg cgccatgcgg ggggcgccgc ggtgcccgac gatggccggc ggccggggcg
     4981 agaaggtgaa catggccgca gtgtaggcag cgccggcgtg ccccggcgcc cggcaagacg
     5041 ccgcattcgc cgggcttgtc cgcattcctg ccaggcccgg gaaaaattcc gcaagatttt
     5101 gcaagcaacc cctttacggg ggacccccgc ccgattacag tgagccacga agtgacgcgg
     5161 accggcttgt gattcggttc acaaagggtc cggccgcgag catcgtgcac caccccgcac
     5221 cgaaaccggc gctcggcaat cccccgagtc caccccgagc cgcacggcct gtccgcgctg
     5281 cccccgggcg cgaacaccgc acgtgcgagc cggccgacgg cgaggctcga aggtcgagac
     5341 agaaagcgag caacgaacat ggactggcgt cagaaggccg cctgcctgga cgaggaccct
     5401 gagctgttct tccccgtggg gacgaccggg ccggccctcg agcaggtcga gcgcgcgaag
     5461 gctgtgtgcg cacgctgcga ggtcgtccag gactgcctgg aatgggccct ggacagcaac
     5521 caggacgcgg gcgtgtgggg cggcatgagc gaggacgagc gccgcacgct gcggcgcagc
     5581 cgtcagcgcc gccgccgcat ggccagctag ctcaccacac cccccggata cccgatgccg
     5641 ggcttccgcc tcacggcgga gcccggcatc gcgcgttcgg cggggcaatt ctgcgatcat
     5701 gcgtcgccgt gaccgactac gagccgtacg tcagtgaccc cgccgaggcg ccgccgcgcg
     5761 agcgcctggc gcgcatgcgc cccacgctgg cgtggctggc cgggatcgtg ctgatcctgg
     5821 cgctcgcgtt cacctgggtg cagggcgccg gctcggagga cccgtccctg a
//
LOCUS       DATMKC010000214         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_301207, whole genome shotgun sequence.
ACCESSION   DATMKC010000214 DATMKC010000000
VERSION     DATMKC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301207"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VNU01_06285"
     CDS             <1..586
                     /locus_tag="VNU01_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWH32263.1"
                     /translation="GWDGAARAVLVVADTLKPTSAEAVTGLRALGLRPVLLTGDNERA
                     ARAIAAQLGIQEVIAEVLPQEKVDVVRRLQADGRVVAMVGDGVNDAAALAQADLGLAM
                     GTGTDVAIEASDLTLARGDLRAAVDAIRLARRTLGTIKGNLFWAFAYNVAALPLAAAG
                     MLNPVIAGAAMAFSSFFVVTNSLRLRGFRSVAGA"
     gene            682..1599
                     /locus_tag="VNU01_06290"
     CDS             682..1599
                     /locus_tag="VNU01_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWH32264.1"
                     /translation="MRFPARLPAPLLALTLSAAACGGGEEPRSDAIEEAASAPAPSEW
                     FTPAEPSHADGDERPNPEATERERDPASSPPPAWLGTRPLERTSSGYGAAQPTPPELV
                     DRRLWTEDILPPPPDERFTSQVVPVPPDIAERTTWKPECPVPLEDLRYVTVAFWGFDG
                     LPHTGEMMLHERVADDVVGVFAKLHAARFPLEYLLLTDDVHNTQPPTGDGNVSSTFNC
                     RPSRGSSSWSEHAKGLAVDINPFHNPYVRGDVVLPELATAYVDRANHRPGMIQPGDVV
                     TRAFAEIGWGWGGNWRSSKDTMHFSASGN"
     gene            complement(1609..>2867)
                     /locus_tag="VNU01_06295"
     CDS             complement(1609..>2867)
                     /locus_tag="VNU01_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWH32265.1"
                     /translation="ASRWLVAAGAVLVQLALGAVYAWSVFARPLEAQMGWTKSQASLP
                     FVVVIGVLFIGTFAGGRIQDRVGPRPVVLAGGLLYAAGVMLASLADQPGELWLLVATY
                     GVMAGIGLGLGYIVPIAMLVKWFPDKRGLITGIAVGGFGAGALVTAPIAEALIDPADV
                     TQTFLALGAGYLVATLLGGALFRDPPAGWSPPGWQGGEARALGEARQYALGEALRTRQ
                     WWLLTAILALNTTAGIALISQASPAAQALTGATPAAAAGLVGILAIFNGAGRVLWAWL
                     SDHAGRMRVFLAMLGVQAVAFFLLARTGSILLFGVLAALVYLAYGGGFGTMPATAADY
                     FGTRHAGAIYGAMIVAWSIGGVAGPLAIAAIAERTGGFSTPFTVIAVLALLSMALPLL
                     TRPPAERGRAPEPRRAAARKPRQRHA"
BASE COUNT          400 a         1121 c         1020 g          326 t
ORIGIN      
        1 cggctgggac ggcgccgcac gcgccgtcct ggtcgtcgcc gacacgctca agccgacctc
       61 cgcggaggcc gtgaccggcc tgcgtgcgct gggcctgcgc cccgtgctgc tcaccggcga
      121 caacgagcgc gcggcgcgcg cgatcgcggc gcagctcggg atccaggagg tgatcgccga
      181 ggtcctgccg caggaaaagg tggacgtcgt ccgccggctc caagcggacg gtcgcgtggt
      241 cgcgatggtc ggtgacggcg tcaacgacgc ggcggcgctg gcccaggcgg acctgggcct
      301 ggcgatgggc accggcaccg acgtggcgat cgaggccagc gacctgaccc tggcccgcgg
      361 cgacctgcgc gccgcggtgg acgcgatccg cctcgcgcgc aggacgctgg gcaccatcaa
      421 gggcaacctg ttctgggcgt tcgcctacaa cgtggccgcc ctgccgctgg ccgccgcggg
      481 catgctcaac ccggtgatcg cgggcgccgc gatggcgttc tccagcttct tcgtggtcac
      541 caacagcctg cgcctgcgcg gcttccgctc cgtcgccggc gcctaacccc cgccccgcct
      601 gacgctgcgg agtcgattgt gtccgatagt ggccacaatc gactccgcaa gcagcgtggg
      661 tgagtgggta cggtgggcgg catgcgtttc ccggcacgcc tgcccgcccc gctgctcgcc
      721 ttgacgctgt ccgccgccgc ctgcggcggc ggcgaggaac cccgcagcga cgccatcgag
      781 gaggccgcct ccgcgcccgc gccctcggag tggttcacgc cggccgagcc gagccacgcg
      841 gacggggacg agcgccccaa cccggaggcg accgagcggg agcgcgaccc cgcctcctct
      901 cccccgccgg cgtggctcgg cacgcggccg ctggagcgca cctcgtccgg ctacggcgcg
      961 gcccagccga cgccgccgga gctggtggac cgccggctgt ggaccgagga catcctgccg
     1021 cccccgccgg acgagcggtt cacctcccag gtcgtccccg tgccgcccga catcgccgag
     1081 cgcaccacgt ggaagccgga gtgccccgtg ccgctggagg acctgcgcta cgtcaccgtg
     1141 gcgttctggg gcttcgacgg cctgccgcac accggcgaga tgatgctcca cgagcgcgtg
     1201 gccgacgacg tggtgggcgt gttcgccaag ctgcacgcgg cgcgcttccc gctggaatac
     1261 ctgctgctca ccgacgacgt gcacaacacc cagcccccca ccggcgacgg caacgtctcg
     1321 agcacgttca actgccggcc gtcgcgcggc tcgtccagct ggtcggagca cgccaagggc
     1381 ctggcggtgg acatcaaccc gttccacaac ccgtacgtgc gcggcgacgt ggtgctgccg
     1441 gagctcgcga ccgcctacgt cgaccgggcc aaccatcggc ccggcatgat ccagcccggc
     1501 gacgtggtga cgcgggcgtt cgccgagatc ggctggggct ggggcggcaa ctggcgctcc
     1561 tccaaggaca ccatgcactt cagcgccagc ggcaactgac gctccgggtc aggcgtgccg
     1621 ctggcgcggc ttgcgcgcgg cggcccggcg aggctccggc gcccgcccgc gctcggccgg
     1681 cggccgcgtc aacagcggca gcgccatcga cagcagcgcc agcacggcga tgaccgtgaa
     1741 cggcgtgctg aacccgccgg tgcgctcggc gatggcggcg atcgccagcg gtccggccac
     1801 gccgccgatg ctccacgcca cgatcatcgc gccgtagatc gcgccggcgt ggcgcgtgcc
     1861 gaagtagtcc gcggccgtgg ccggcatggt gccgaacccg ccgccgtagg ccaggtagac
     1921 cagcgccgcc agcacaccga acagcaggat gctcccggtg cgcgccagca ggaagaacgc
     1981 caccgcctgc acgcccagca tcgccaggaa cacgcgcatg cgcccggcgt ggtcggacag
     2041 ccacgcccac agcacccgcc cggcgccgtt gaagatcgcc aggatgccca ccagccccgc
     2101 cgcggccgcc ggcgtcgccc cggtgagcgc ctgcgccgcc ggggaggcct gggagatcag
     2161 cgcgatgccc gccgtggtgt tcagcgcgag gatcgccgtc agcagccacc actgccgggt
     2221 gcgcagcgcc tcgccgagcg cgtactggcg ggcctcgccc agcgccctcg cctcgccgcc
     2281 ctgccagccg ggcggcgacc agccggccgg cgggtcgcgg aacagcgcgc cgccgagcag
     2341 cgtcgcgacc aggtagccgg cgcccagggc caggaacgtc tgcgtgacgt cggcggggtc
     2401 gatgagcgcc tcggcgatcg gcgcggtcac cagcgcgccg gcgccgaagc cgccgaccgc
     2461 gatccccgtg atgaggccgc gcttgtccgg gaaccacttc accagcatcg cgatcggcac
     2521 gatgtagccc aggcccaggc cgatgccggc catgacgccg taggtcgcga ccagcagcca
     2581 cagctcgccc ggctggtcgg ccagcgaggc cagcatcacg cccgccgcgt acagcaggcc
     2641 gccggccagc accacggggc gcgggcccac gcggtcctgg atgcgcccgc ccgcgaacgt
     2701 gccgatgaac agcacgccga tcaccacaac gaacggcagc gacgcctggc tcttggtcca
     2761 gcccatctgc gcttccagcg gccgcgcgaa cacgctccat gcgtacacgg cgccgagcgc
     2821 gagctgcacc aggacggcgc cggcggcgac gagccagcgg ctggcgg
//
LOCUS       DATMKC010000215         5138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_287700, whole genome shotgun sequence.
ACCESSION   DATMKC010000215 DATMKC010000000
VERSION     DATMKC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287700"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VNU01_06300"
     CDS             complement(<1..662)
                     /locus_tag="VNU01_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32266.1"
                     /translation="MTDLDTRLREASHGVLLCWDGAGASARLLALASPAWWDGAGVWM
                     LAPMLAAGAPEALRGRPPCALLLDDGDGGLALRGSARLFGLHDPVGLALHAPAIAGAM
                     AALALRGAATLRRALPGTLPREPALRVAADAMEPFAVAAPRAAGGGIAPALPADVPPD
                     VRRDLAGVRDVVVLAAGSILTQRAVWGAGLSLGGARVAAGRAAVVARSGRTGLALEGE
                     VGA"
     gene            732..1037
                     /locus_tag="VNU01_06305"
     CDS             732..1037
                     /locus_tag="VNU01_06305"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWH32267.1"
                     /translation="MFVAINVLNVPPQMRETLEQRFAARAGEVDKMEGFQAFELLRPV
                     EGQDNYLVYTRWDTKEHFQAWLDSQAFSQGHAAHASGGPAASGSQVWTFEVAEHKER"
     gene            1045..1605
                     /locus_tag="VNU01_06310"
     CDS             1045..1605
                     /locus_tag="VNU01_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWH32268.1"
                     /translation="MDLYEGLLTRRSVGKVTEPGPDDAELRRLLAAAAAAPDHGLHRP
                     WRFVVLRGAARESLGEAFADALRARRPDCDEAALAADRAKPLRAPVLVAVISAPVPSP
                     KAPEWEQLASAAAAAQNLLLAAHAGGWGAMWRTGWFVDAPEVRAELGLAESERCVGLV
                     YLGTPAAAPPPRPAVDVDALTSWRDS"
     gene            1602..2372
                     /locus_tag="VNU01_06315"
     CDS             1602..2372
                     /locus_tag="VNU01_06315"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWH32269.1"
                     /translation="MTWVLRTALTMEQLEAHLPALEAAGLLGMDEQAGVTSAYFPERA
                     ELGVAGTWEPLPERDWNAEARRRLTAVRVGGLVVTPPWLPQTPPDAVAVVVDPAQAFG
                     TGHHETTTACLAALQEVELAGRTVLDVGTGSGVLAIAAALLGAGPVLAVDNDPLAVEA
                     ARHNAARNSVAIEARLATPDEVDGVYDVVLANLDTATIGAVAGDLAARLAPGGCLIAS
                     GVSVERLDDGVAALEAAGLVVWARPGAEWVLLSARHAS"
     gene            2704..3480
                     /locus_tag="VNU01_06320"
     CDS             2704..3480
                     /locus_tag="VNU01_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HWH32270.1"
                     /translation="MHSTHARAREPRVETVRLHGLAHGGEAVGRLEDGKAVFVPFAIP
                     GELATVRVTDERARWARAELVEVLEPSPDRVEPACPLFGRCGGCALQHIAGPRRRGLL
                     RQVLVDQLQRIGGLDDPPVAEVRDAGEWAYRTSARFAIARDGALGFRAAGSHDIVAVE
                     HCPLLHPAAQAVRSEVGPRYERPGPEVEEVMVRAAARGDGATLVTLPGASGATITERV
                     GGLEFRVSPGSFFQQNTVGAEILLALVREAAAAAPGRAPR"
     gene            3480..3668
                     /locus_tag="VNU01_06325"
     CDS             3480..3668
                     /locus_tag="VNU01_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32271.1"
                     /translation="MARLAGLRPRVIVYVSCDPAALARDARTLAEAGYTLEAATPVDQ
                     FAQTAHLETVAVFRPAGA"
     gene            3699..>5138
                     /gene="dacB"
                     /locus_tag="VNU01_06330"
     CDS             3699..>5138
                     /gene="dacB"
                     /locus_tag="VNU01_06330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HWH32272.1"
                     /translation="MHTMRARAFLLLVAGLLLGGGLFTLTSQDGQAAPPAVAAATASP
                     PSTTAPAAPPTLAPPPAPEPTAAPAEVAPPPPPSLTQRVDALLADPALAGVAFGIAVR
                     DEAGRPVLERGGAAALLPASTVKQATAAAALGRLGAGFRFTTRVVAPAGPTPDGTLNG
                     DLVLVGSGDPALATETFRGQVSPERPATPLEALADQIAARGIRRITGGVLGDPTVFAH
                     QPLASGWRPRYLEELDATYTSGLTVDAGRKLFVEGGKLRAVASPDPASEAAAALLTAL
                     RNRGVQVDGGVATVTAPTQHGLILASVQSPPLDELLRWMVQRSDNHIADALFRTLGAT
                     AGDATWTGAEAATRAALAPLGLDWTGVRLADGSGLSRDDRIPADFLAALDAAVMRTPS
                     AERWASWQAVAGESGTLRKRLRGTLAEHRLRGKTGSLEDVRALAGSVSGPVLPRSRCS
                     ASVPRSRLRSVPLSPATACQLAQRSADGVR"
BASE COUNT          571 a         2011 c         1972 g          584 t
ORIGIN      
        1 gcgccgacct caccctccag tgccaggccg gtgcggccgg agcgcgccac gacggcggcg
       61 cgcccggcgg ccacccgggc gccgccgagg gacaggcccg ccccccacac ggcccgctgc
      121 gtcaggatcg agcccgccgc cagcaccacg acgtcgcgca cgccggcgag gtcgcggcgc
      181 acgtccggcg gcacgtcggc cggcagcgcg ggcgcgatcc ccccgcccgc cgcccgcggg
      241 gccgcgacgg cgaagggctc catcgcgtcg gccgccacgc gcagcgccgg ctcccgcggc
      301 agcgtgcccg gcagggcgcg gcgcagggtg gcggcgccgc gcagcgccag cgccgccatg
      361 gcgccggcga tggccggggc gtgcagcgcc aggccgaccg ggtcgtgcag gccgaacagc
      421 cgcgcgctgc cgcgcagcgc cagtcccccg tcgccgtcgt ccagcagcag cgcgcacggc
      481 ggcctgccgc gcagcgcctc gggcgcccca gcggcaagca tgggagcgag catccacacc
      541 ccggcgccgt cccaccaggc cggtgacgcc agagccagca ggcgggcgga ggcccccgcg
      601 ccgtcccagc acagcagcac gccgtgggag gcttcgcgca gccgggtatc gaggtcggtc
      661 atggacgcga atggtgcccg cttccaccca caaggttcaa tgcgctccca tccccttgcg
      721 aaaggacgac gatgttcgtc gcgatcaacg tgctcaacgt gccaccgcag atgcgcgaga
      781 ccctggagca gcgcttcgcc gcgcgcgccg gcgaggtgga caagatggag ggcttccagg
      841 ccttcgagct gctgcgcccg gtcgagggcc aggacaacta cctggtctac acgcgctggg
      901 acaccaagga gcacttccag gcgtggctgg actcccaggc gttctcccag ggccacgccg
      961 cgcacgcctc gggcgggccg gccgccagcg gctcgcaggt gtggaccttc gaggtggccg
     1021 agcacaagga gcgctgagcg ccccgtggac ctctacgagg gactgctcac gcggcgcagc
     1081 gtcggcaagg tcaccgagcc aggcccggac gacgccgagc tgcgcaggct gctcgccgcg
     1141 gccgccgcgg cgcccgacca cggcctgcac cggccctggc gcttcgtggt gctgcgcggc
     1201 gccgcccggg agtccctggg cgaggcgttc gccgacgccc tgcgcgcccg ccgcccggac
     1261 tgcgacgagg cggcgctggc ggccgaccgg gccaagccgc tgcgcgcgcc ggtgctcgtc
     1321 gccgtgatca gcgcgccggt gccctcgccc aaggcccccg agtgggagca gctcgcctcg
     1381 gccgccgcgg cggcgcagaa cctgctgctc gccgcccacg ccggcggctg gggcgcgatg
     1441 tggcgcaccg gctggttcgt ggacgcgccc gaggttcgcg ccgagctggg gctggccgaa
     1501 agcgagcgtt gcgtcggcct ggtgtacctg ggcacccccg cggcggcgcc gccgccccgg
     1561 ccggcggtcg acgtcgacgc gctcacgtcg tggagggact cgtgacctgg gtgctgcgga
     1621 ccgcgctgac catggagcag ctcgaggccc acctgcccgc cctggaggcg gcgggcctgc
     1681 tcggcatgga cgagcaggcc ggcgtcacca gcgcctactt ccccgagcgg gcggagctcg
     1741 gcgtggcggg gacctgggag ccgctgcccg agcgggactg gaacgccgag gcgcgccgcc
     1801 ggctcaccgc cgtgcgcgtg ggcggcctgg tggtgacgcc gccgtggctg ccccagacgc
     1861 cgccggacgc cgtggccgtg gtggtcgacc ccgctcaggc cttcggcacc ggccaccacg
     1921 agaccaccac ggcgtgcctg gcggcgctgc aggaggtgga gctcgccggc cgcaccgtgc
     1981 tggacgtggg caccggcagc ggcgtcctgg cgatcgccgc cgcgctgctg ggcgccgggc
     2041 ccgtgctggc cgtggacaac gacccgctgg cggtggaggc cgcgcgccac aacgcggcgc
     2101 gcaacagcgt ggcgatcgag gcgcgcctgg cgacccccga tgaggtcgac ggtgtctacg
     2161 acgtcgtcct ggccaacctg gacaccgcga cgatcggcgc ggtggcgggc gacctggcgg
     2221 cgcggctggc gcccggcggc tgcctgatcg cctccggcgt gagcgtggag cgcctggacg
     2281 acggcgtcgc ggcgctggag gcggcgggcc tggtggtgtg ggcgcggccg ggggccgagt
     2341 gggtcctgct cagcgcccgc cacgcgtcct gaggcccggg gagcatcagc cgggcggggc
     2401 cgccgcgccc cgcccggcga tgcgtcgagg ttgtgcgggg cactgtgacc gatgcgcgtg
     2461 ccgccgcggt gcgatcacga cgagtctttt cgcttcccgg cggaccggtg gcccccaccc
     2521 gcacacgctg cggggaaacc agagaggcgg agccgcccgc cggctccgcc tctccgtacc
     2581 gcccccggta tggtgtattc gctggcaccc cgttttgtcc ctgcctttcc gttcaccttt
     2641 tgcaaattct ggcttctgcc cggtttgccc ctctgtctgc gagccgagat gccccgccgc
     2701 cccatgcatt ccacgcatgc ccgcgcgcgc gaaccgcgcg ttgagaccgt ccgcttgcac
     2761 ggtctggcgc atggcggcga agccgtgggc cgcctggagg acggcaaggc ggtgttcgtc
     2821 ccgttcgcga tcccgggcga gctggccacg gtgcgcgtca ccgacgagcg cgcccgctgg
     2881 gcgcgcgcgg agctggtcga ggtgctggag ccgtccccgg accgggtgga gccggcctgc
     2941 cccctgttcg gccgctgcgg cggctgcgcc ctgcagcaca tcgccggccc ccgccgccgc
     3001 gggctgctgc gccaggtgct ggtggaccag ctccagcgca tcggcgggct ggacgacccg
     3061 ccggtcgcgg aggtgcggga cgcgggcgag tgggcctacc gcaccagcgc gcgcttcgcg
     3121 atcgcccgtg acggcgcgct ggggttccgt gccgcgggca gccacgacat cgtcgcggtg
     3181 gagcactgcc cgctgctgca ccccgccgcc caggcggtgc gcagcgaggt cgggccgcgc
     3241 tacgagcgcc cggggccgga ggtcgaggag gtcatggtcc gcgccgccgc ccgcggtgac
     3301 ggcgccacct tggtgaccct gccgggcgcc tccggcgcca ccatcaccga acgggtgggc
     3361 ggactggagt tccgcgtgtc gccgggcagc ttcttccagc agaacaccgt cggcgcggag
     3421 atcctgctgg cgctggtgcg cgaggccgcg gcggccgcgc cggggcgggc gccgcggtga
     3481 tggcccggct cgccgggctg cggccgcggg tgatcgtgta cgtctcgtgc gacccggccg
     3541 cgctggcccg cgacgcgcgc accctcgcgg aagccggcta cacgctggag gccgcgacgc
     3601 cggtggacca gttcgcccag acggcgcacc tggaaaccgt cgccgtcttt cggcccgcgg
     3661 gggcctgaga ccgccgggcg cgcctacact cggtcgggat gcacaccatg cgggcgcggg
     3721 cctttctgct cctcgtggcc ggcctcctgc tggggggcgg cctgttcacg ctgacgtccc
     3781 aggacggtca ggccgcgccg cccgcggtgg ccgccgccac cgcctcgccg ccgagcacca
     3841 ccgccccggc cgcgccgccg acgctggcgc cgcccccggc gcccgagccc accgccgcac
     3901 cggccgaggt tgcgccgccg cccccaccgt cgctgaccca gcgggtcgac gcgctgctgg
     3961 ccgacccggc actggccggg gtggcgttcg gcatcgcggt gcgcgacgag gccgggcgcc
     4021 ccgtgctgga gcgcggcggc gccgccgccc tgctgcccgc gtccacggtg aagcaggcga
     4081 ccgccgccgc ggcgctcgga cggctgggcg ccggcttccg cttcaccacg cgcgtggtcg
     4141 cgccggccgg ccccacaccc gacggcacgc tcaacggcga cctcgtgctg gtcggctcgg
     4201 gcgacccggc gctggccacg gagacgttcc gggggcaggt ctccccggaa cggcccgcca
     4261 ccccgctgga agcgctggcc gaccagatcg cggcccgggg gatccgccgg atcaccggcg
     4321 gcgtcctggg cgaccccacg gtgttcgccc accagccgct ggccagcggg tggcggccgc
     4381 gctacctgga ggagctggac gccacctaca cctccggcct cacggtcgac gccgggcgca
     4441 agctgttcgt ggagggcggc aagctgcggg ccgtcgccag ccccgacccg gccagcgagg
     4501 cggccgcggc gctgctgacg gccctgcgca accgcggcgt gcaggtcgac gggggtgtcg
     4561 cgacggtcac cgcgccgacg cagcacggcc tcatcctggc cagcgtgcag agcccgccgc
     4621 tggacgagct gctgcgctgg atggtgcagc gcagcgacaa ccacatcgcc gacgcgctgt
     4681 tccgcacgct gggcgccacg gccggcgacg ccacctggac gggcgccgag gccgccaccc
     4741 gcgccgcgct ggcgccgctg ggcctggact ggacgggcgt gcgcctggcc gacggcagcg
     4801 gcctgtcgcg cgacgaccgc attccggccg acttcctggc ggccctggac gccgccgtca
     4861 tgcgcacgcc gtcggcggag cgctgggcca gctggcaggc cgtcgcgggc gagagcggca
     4921 cgctgcgcaa gcggctgcgc ggcacgctgg ccgagcaccg gctgcgcggc aagaccggct
     4981 cgctggagga cgtgcgggcc ctggccgggt ccgtcagcgg gccggtcttg ccgcgcagcc
     5041 ggtgctcggc cagcgtgccg cgcagccgct tgcgcagcgt gccgctctcg cccgcgacgg
     5101 cctgccagct ggcccagcgc tccgccgacg gcgtgcgc
//
LOCUS       DATMKC010000216         5011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_120648, whole genome shotgun sequence.
ACCESSION   DATMKC010000216 DATMKC010000000
VERSION     DATMKC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120648"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VNU01_06335"
     CDS             <1..406
                     /locus_tag="VNU01_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32273.1"
                     /translation="PTLDEVAATCPGLPAPRLHRNPDREGVTGMATWLWADGHAALRS
                     TSAIRGYPVSCTAVPERWTWYSGDGASYSRSGPGAPPPDHAAEHTYRRKGAYTQRLVV
                     RWRLETSHGTRAASRQTRRGYQVAEIRGALVE"
     gene            469..1653
                     /locus_tag="VNU01_06340"
     CDS             469..1653
                     /locus_tag="VNU01_06340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HWH32274.1"
                     /translation="MNALDIVLLLAIAYSALRGFRQGAVSQIAAFGGAMVGLGLGAAL
                     APRIAGSIVSGGPGSSLALGTLGLLMLFVFIGQGVGFGVGMRLRAAAAHIGAEPVDRT
                     AGVVVGLAGLIATVWLLGAALSQGPSVVVAQQIRESQVVTTIDQALPPAPDIFAQLGR
                     YLDQHGFPQVFSGLGGGTTAPPVDPPSSAAVAAAQAAGQPSTVQVQATGCGGISSGTG
                     WVTRPGFVVSNAHVVAGGELVTVRDASGTHDAAVIHFDPDLDLAVLAVPTLGAPPIGW
                     ASTPADRGVEGATLGFPGGQRELAVKPATVRDRGEALGRDIYGRGLASREILTLAANV
                     QQGDSGGPFVTAAGEVGGVVFAASPHEPGVGYALTAERVRPDVDAAIGRNSSVPTGAC
                     RY"
     gene            1699..2337
                     /locus_tag="VNU01_06345"
     CDS             1699..2337
                     /locus_tag="VNU01_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWH32275.1"
                     /translation="MGTDARTLTRPEAVGRARALNRLTIGWNVVEAFVALAAGVAAGS
                     ISLIGFGLDSTIEVSAALVLAWRLRQEQRTGCTQPSDRRATRLIAVSFLLLAAYVGAQ
                     AVRDLAGRADPEASAVGIAVTALSLAVMPVLARAKRRLAPVMGSRAVEAEAAQTNICA
                     ILSGVVLAGLAANALLGWWWADPSAALVVAGIAAFEGRRAWRADALADTCCA"
     gene            complement(2338..3693)
                     /locus_tag="VNU01_06350"
     CDS             complement(2338..3693)
                     /locus_tag="VNU01_06350"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HWH32276.1"
                     /translation="MSANPALGPWTPDSWQAYPADQQPEWPDDAQVKDVLSTLAGYPP
                     IVFAGEARDLRAKLADVAAGKAFLLQGGDCAETFSAFNANNIRDKLKVILQMAVVLTY
                     GAQMPVVKVGRIAGQFAKPRSANTETIDGVELPSFRGDAVNDITFTPEARVPDPARLL
                     RVYNQSAATLNLLRAFTRGGFADLERVHAWNQEFVANSAQGQRYEQLADELERALKFL
                     IACGVDVKSDPRFHEVDFYTSHEALLLGYEQALTRQDSLTGEWYDTSAHMLWIGERTR
                     NPDGAHVHFLSGVNNPIGVKLGPTSTVDDAVALADRLNPGNVPGRLTFITRMGVDKVE
                     SALPPLVRGVRDAGLNVVWSVDPMHGNTISVAGYKTRRFEDVLQEVRVFFAVHQQEGT
                     VPGGLHMELTGEDVTECLGGAQDIADLDLQTRYETACDPRLNNQQSLELAFQVAEMLR
                     E"
     gene            3773..4390
                     /locus_tag="VNU01_06355"
     CDS             3773..4390
                     /locus_tag="VNU01_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH32277.1"
                     /translation="MCHHDRQSAHTGGAAISTEQALLDAAERVLVERGLQATKVEDIT
                     SEAGVAKGTFYLYFATKEDVIRAVQRRHFQTMLDNVAESAARLHEADFWAVADALIDT
                     MVAYDLEHRQWYRKVVQGWAPPPSEFANERADMQHLVATAIKQGIADGHCADVDTDVT
                     SLLLCSAIEGSLHQICMTEADPDASRLAAGLKDFVRRVLSPRPTD"
     gene            4428..>5011
                     /locus_tag="VNU01_06360"
     CDS             4428..>5011
                     /locus_tag="VNU01_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH32278.1"
                     /translation="MRIQGKVAVVTGGASGLGRATAEGLVQAGASVVVCDLPSSDGEA
                     VAKELGERARFAPTDVTSETDVQAAVELAAETFGGLHIAVNCAGVGWAQRVLGKQGAH
                     SLELFAKVVQVNLIGTFNVIRLAAEQMVKAEPEDGERGVIVNTASIAAYDGQVGQAAY
                     SASKGGVVGLTLPVARDLAQALIRVVTIAPGTFDT"
BASE COUNT          639 a         1839 c         1841 g          692 t
ORIGIN      
        1 gccgacgctc gacgaagtcg ccgccacgtg ccccgggctg cccgccccgc ggctgcaccg
       61 gaacccggac cgggagggcg tcacgggcat ggcgacgtgg ctgtgggcgg acgggcacgc
      121 ggcgctgcgg tccacgtcgg ccatccgcgg ctacccggtg agctgcaccg ccgtgccgga
      181 gcgctggacc tggtacagcg gcgacggcgc cagctacagc cgttccggcc cgggtgcgcc
      241 gccgccggac catgccgccg agcacaccta ccggcgcaag ggcgcctaca cccagcggct
      301 ggtggtgcgc tggcgcctgg agaccagcca cggcacgcgc gccgccagcc gccagacgcg
      361 gcgcggctac caggtggcgg agatccgcgg cgcgctggtg gagtagccgg cggcggcgtc
      421 aacggatccg gtcgcgcgga acaggtcagc ggctaggctt gcgcctccat gaacgccctc
      481 gacatcgtgc tgctccttgc gatcgcgtac tcggcgctgc gcggcttccg ccagggggcc
      541 gtctcgcaga tcgccgcgtt cggcggggcg atggtgggcc tgggcttggg cgccgccctg
      601 gcgccgcgca tcgccggctc catcgtgagc ggcggccccg ggtcctcgct ggcgctgggc
      661 acgctgggcc tgctgatgct gttcgtcttc atcggccagg gcgtcggctt cggcgtcggg
      721 atgcggctgc gggcggcggc cgcccacatc ggcgccgagc cggtggaccg caccgccggg
      781 gtcgtcgtcg ggctggcggg cctgatcgcg accgtgtggc tgctcggcgc ggcgctgtcg
      841 cagggaccgt cggtggtcgt cgcccagcag atccgcgagt cgcaggtcgt gaccacgatc
      901 gaccaggcgc tgccgccggc gccggacatc ttcgcccagc tcggccgcta cctggaccag
      961 cacgggttcc cgcaggtgtt cagcggcctg ggtggcggga cgaccgcgcc gccggtcgac
     1021 ccgccgagca gcgcggcggt cgcggccgcg caggcggccg ggcagccgag caccgtgcag
     1081 gtgcaggcga ccgggtgcgg cggcatctcg tccggcaccg ggtgggtcac gcgcccgggg
     1141 ttcgtggtca gcaacgccca cgtcgtggcg ggcggcgagc tggtcacggt gcgcgacgcc
     1201 agcggcacgc acgacgccgc ggtcatccac ttcgacccgg atctcgacct cgccgtgctg
     1261 gcggtgccca cgctcggcgc gccgcccatc ggctgggcgt ccacgccggc cgaccggggc
     1321 gtagaggggg cgacgctcgg cttcccgggc ggccagcgcg aactggcggt gaaacccgcg
     1381 accgtccgtg acaggggaga ggcgctcggg cgcgacatct acgggcgcgg cctggcctcc
     1441 cgcgagatcc tgacgctggc ggccaacgtg cagcagggcg actcgggcgg cccgttcgtg
     1501 acggccgccg gcgaggtcgg cggcgtggtg ttcgccgcct cgccgcacga gcccggcgtc
     1561 ggctacgcgc tcaccgcgga gcgggtgcgt ccggacgtcg acgcggcgat cgggcgcaac
     1621 tcctcggtgc cgaccggcgc ctgccgatac tgacgcgcgc gcgtgtcccc tatgcccccg
     1681 gggggtatag tggcgcggat ggggaccgac gcgcgcacgc tcacccggcc cgaggccgtg
     1741 ggccgcgccc gcgcgctcaa ccggctgacc atcgggtgga acgtcgtgga ggcgttcgtc
     1801 gccctggccg ccggcgtcgc cgccggctcc atcagcctca tcggcttcgg cctcgactcg
     1861 accatcgagg tgtccgcggc cctggtcctg gcgtggcgcc tgcgccagga gcagcgcacc
     1921 ggctgcacgc agccgtccga ccgccgcgcc acccggctga tcgccgtcag cttcctgctc
     1981 ctggccgcct acgtcggcgc gcaggccgtg cgcgacctcg ccggccgcgc cgacccagag
     2041 gccagcgccg tcggcatcgc ggtcaccgcg ctgtcgctgg ccgtgatgcc ggtgctggcg
     2101 cgggccaagc gccgcctggc gccggtcatg ggctcgcgcg ccgtggaggc cgaggccgcg
     2161 cagacgaaca tctgcgcgat cctgtccggt gtggtgctgg ccggcctcgc cgcgaacgcg
     2221 ctgctgggct ggtggtgggc ggacccgtcc gccgccctgg tggtcgcggg catcgcggcg
     2281 ttcgaggggc ggcgcgcctg gcgcgccgac gccctcgccg acacctgctg cgcctagcta
     2341 ctcgcggagc atctcggcga cctggaacgc cagctccagc gactgctggt tgttcagccg
     2401 ggggtcgcag gccgtctcgt agcgggtctg caggtccagg tcggcgatgt cctgcgcgcc
     2461 gcccaggcac tcggtcacgt cctcgccggt gagctccatg tgcagcccgc cgggcaccgt
     2521 gccctcctgc tggtgcaccg cgaagaacac gcgcacctcc tggagcacgt cctcgaagcg
     2581 gcgcgtcttg tagccggcca cggagatcgt gttgccgtgc atcgggtcca ccgaccacac
     2641 cacgttcagg ccggcgtcgc gcacgccgcg caccagcggg ggcagcgccg actccacctt
     2701 gtccacgccc atgcgcgtga tgaacgtcag ccggcccggc acgttgcccg gattgagccg
     2761 atcggccagc gccaccgcgt cgtcgacggt cgacgtcggc cccagcttca cgccgatggg
     2821 gttgttgacg ccggacagga agtgcacgtg cgcgccgtcg gggttccgcg tgcgctcgcc
     2881 gatccacagc atgtgcgccg acgtgtcgta ccactcgccc gtcagcgagt cctggcgcgt
     2941 cagcgcctgc tcgtagccca gcagcagcgc ctcgtgggag gtgtagaagt ccacctcgtg
     3001 gaagcgcggg tcgcttttga cgtccacgcc gcaggcgatg aggaacttga gcgcccgctc
     3061 cagctcgtcg gcgagctgct cgtagcgctg gccctgcgcc gagttggcca cgaactcctg
     3121 gttccaggcg tgcacgcgct ccaggtccgc gaacccgccg cgcgtgaacg cgcgcagcag
     3181 gttgagcgtc gcggcggact ggttgtagac gcgcagcagc cgcgcgggat ccggcacgcg
     3241 ggcctcgggg gtgaacgtga tgtcgttcac cgcgtcgccg cggaagctcg gcagttccac
     3301 gccgtcgatg gtctcggtgt tggccgagcg cggcttggcg aactgcccgg cgatgcggcc
     3361 gaccttgacc accggcatct gcgcgccgta ggtgagcacc accgccatct gcaggatgac
     3421 cttgagcttg tcgcggatgt tgttggcgtt gaacgccgag aacgtctccg cgcagtcgcc
     3481 gccctggagc agaaacgcct tgccggccgc gacgtcggcc agcttggcgc gcaggtcccg
     3541 cgcctcgccc gcaaacacga tggggggata gccggccagc gtggacaaaa cgtccttcac
     3601 ctgggcgtcg tcgggccatt cgggctgctg gtctgccggg tacgcctgcc aagagtcggg
     3661 cgtccacggc cccaatgcgg ggttcgcgct catgcctcac atggtcacat tcgggcgacc
     3721 cgcgcgcaaa gggtctttgg cggcgcgcct cccgcgggac ggcggaagcg atgtgtgcca
     3781 ccatgaccgg cagtcagccc acaccggagg tgccgcaatc agcaccgagc aggccctgct
     3841 ggacgccgcc gagcgcgtcc tggtcgaacg cggcctccag gccaccaagg tggaggacat
     3901 cacgtccgag gccggcgtcg ccaagggcac cttctacctg tacttcgcca ccaaggaaga
     3961 cgtcatccgc gccgtccagc gccgccactt ccagaccatg ctggacaacg tcgccgagtc
     4021 cgcggcccgc ctgcacgagg cggacttctg ggccgtcgcg gacgcgctga tcgacacgat
     4081 ggtcgcctac gacctggagc accggcagtg gtaccgcaag gtcgtgcagg gctgggcgcc
     4141 cccgccgtcg gagttcgcca acgagcgcgc cgacatgcag cacctggtcg ccaccgccat
     4201 caagcagggc atcgccgacg gccactgcgc cgacgtggac accgacgtga cgtcgctgct
     4261 gctgtgctcg gccatcgagg gctccctgca ccagatctgc atgaccgagg ccgatccgga
     4321 cgcgagccgc cttgccgcgg ggctgaagga cttcgtccgc cgcgtgctgt ccccccggcc
     4381 caccgactag gctctgcgcc gccacagcgt tcgtcgagga gggcagcatg cgcatccagg
     4441 gcaaggtcgc ggtcgtcacg gggggcgctt cgggcctcgg cagggcgacc gccgagggcc
     4501 tggtccaggc cggcgcgtcc gtggtggtgt gcgacctgcc gagctcggac ggcgaggccg
     4561 tcgccaagga gctcggcgag cgcgcacgct tcgcccccac ggacgtcacc agcgagaccg
     4621 acgtgcaggc cgcggtggaa ctcgcggccg agaccttcgg tgggctgcac atcgccgtga
     4681 actgcgccgg ggtcggctgg gcgcagcgcg tgctcggcaa gcagggcgcg cactccctgg
     4741 agctgttcgc caaggtggtg caggtcaacc tgatcggcac gttcaacgtg atccgcctgg
     4801 ccgccgagca gatggtcaag gccgagccgg aggacggcga gcgcggcgtg atcgtcaaca
     4861 ccgcgtccat cgccgcctac gacggccagg tcggccaggc cgcctacagc gcgtccaagg
     4921 gcggcgtcgt cggcctgacg ctgcccgtgg cgcgcgacct cgcgcaggcg ctgatccggg
     4981 tggtgacgat cgcccccggc acgttcgaca c
//
LOCUS       DATMKC010000217         8679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_168296, whole genome shotgun sequence.
ACCESSION   DATMKC010000217 DATMKC010000000
VERSION     DATMKC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168296"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VNU01_06365"
     CDS             complement(<1..735)
                     /locus_tag="VNU01_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH32279.1"
                     /translation="MCSSVGVEIDAALATADLHGLLDVAERALRRAKTLPLGGAVSDV
                     ALTQAVQRLEAVSRVAESHKCAVVVEIEQRHAARGQSAASTEDLLTRVLHLTPGEAKA
                     RTELAAGLKQLPDTAAAFADGRMGLGQAAVAVKKAAEVAGRDDAEVLLATIDRTASTV
                     GQTTNRTRLAREIDTAVAKTGVDVLAERERRAFQDRKLQWSVRDGMDVLTAHLDPVGA
                     ATVRGMLDALSDKTGDNDSRTFVQRQA"
     gene            928..1001
                     /locus_tag="VNU01_06370"
     tRNA            928..1001
                     /locus_tag="VNU01_06370"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Gly,seq:tcc)
     gene            1213..2262
                     /locus_tag="VNU01_06375"
     CDS             1213..2262
                     /locus_tag="VNU01_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32280.1"
                     /translation="MVGTRTASATRRSLTRREHLLTVGFGLWMVVGLFLDGWAHDNNQ
                     PESFFTPWHAVLYSGFVAAGATALLIAWRDRRPGTPWARTLPRGHGLTLAGLALFGAG
                     GAGDLVWHELLGVEVGLEALLSPTHLLLLVGGLLALSAPFRAAWSEPVTDPESLRSFA
                     PTVLTLAFLTAVFAFFMLYFSPFANDAAGTAFERVHGASHTHPSSDLGELQQLLGVAS
                     ILMTTVLLVVPAHLLLRRWEPPAGTFTLFFGVVVTLLQASGEYAQPSMLLLGPLAGAA
                     TDRLLRRTAPVVGVAGGLMLLWLGYFALYALEEGAVAWTAELWTGTTVMAGLLTAGLA
                     LPALRAPVPPGTDGP"
     gene            complement(2300..4627)
                     /locus_tag="VNU01_06380"
     CDS             complement(2300..4627)
                     /locus_tag="VNU01_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWH32281.1"
                     /translation="MAQLGEKVGGSPARAISALRQFLPAGDSLDAPSWRKRHRAICGV
                     LWAHVPAVAAFALVRGETAVHSLGEAAILAVLAAGASVRVWPDAARAVMATLGLVSAS
                     AIAVHLSGGVIEMHFHFFVVVVIASLYQSWVPFLVALAFVVLHHGIVGQLAPESVFNH
                     PAALAGAWRWAAVHGAFVLAESAACLAAWRLNETALEGERAARVALERSNADLLVAQR
                     LSSIGSWDWEIATDTVWWSRELYTIFGVQPDEFLPSVAGFLAHVHEDDRGRVALLVEQ
                     AGSVPAGDLEFNCRVLRADGTERIVHALGHAAVGDDGRPTRLMGTVQDITERKALERE
                     IEYRAFHDPLTDLANRALFMDRVRHALAVRERTGASIAVLYLDLDDFKTVNDTLGHSA
                     GDALLVQVAHRLCDSVRTSDTVARLGGDEFAVLLENTDTGQAARAAEAVLDALQEPVQ
                     VDRAHLLVRTSIGVVVAERSIESEELLRRADVAMYEAKRQGKHMWRAYGESMQSSLST
                     MLSLEAQVPAAVEAGEFLLHVQPVVSMGDGTVRALEVLVRWRHPQHGILPASAFVPVA
                     EETGVIGLLGEWILTEAVQQACWMRERLGRVVPLSLSVSPRQLHGDVAGLIEQHLAAA
                     DLAADCLLVEVGEDCLGPHSAAVPKLAALRAAGARVVVGDFGTGRSSLAGLRQMPVDA
                     LKIDRSFVRNVVGGPEESAMAQAIVKLAALFGLRTIAEGVETAEQAAVLRSLGCDDAQ
                     GHHFARPMDPRRFATEVEAGRLRLPDAHQGSLHPA"
     gene            complement(4819..5382)
                     /locus_tag="VNU01_06385"
     CDS             complement(4819..5382)
                     /locus_tag="VNU01_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWH32282.1"
                     /translation="MSANVTQTVHPELAAMGADRIEEEHRAYRARYALALARISLGWV
                     FLWAFLDKTFGLGFATTTENAWVNGGSPTYGFLNFGTEGKVFHDFFAGLAGPVADWTF
                     MLGLLGIGAALILGIGMRAAAASGVLLMTAMWAASLRLDNNPFMDDHLVYAIVLVALA
                     MFGAGRTWGLGRAWEKLPLVQRHRYLI"
     gene            complement(5537..6574)
                     /locus_tag="VNU01_06390"
     CDS             complement(5537..6574)
                     /locus_tag="VNU01_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWH32283.1"
                     /translation="MKAIVYHGPGDKRWEDVPDPRIVDDTDAIVRVDAVTICGTDLHI
                     LKGDVPTVAEGRILGHEAVGTVEEVGPGVKTLKPGDRVLVSCITSCGRCEYCRAGRYG
                     QCTGGGGWILGHLVDGTQAERVRVPFADTSTYPLPAGVSDEALLMLADILPTGYEVGV
                     LNGAVGPGKTVAVVGAGPIGLAAITGAKLLSPSQIIAIDLADSRLEAAKQMGATATIN
                     SGREHAIDRVMELTGNGVDVAIEAVGVPATFELCADLVRPGGRVANVGVHGEPATLHL
                     EKLWIRDVTITTGLVDTFSTPTLLRLIGAQLVDPRPFVTHHFRMGEFLDAYDVFARAG
                     ETGALKVVVER"
     gene            6759..7178
                     /locus_tag="VNU01_06395"
     CDS             6759..7178
                     /locus_tag="VNU01_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWH32284.1"
                     /translation="MRVREWMSPDPVTVTPSTTVREARRLLRHYGIRHLPVLGRDGSL
                     AGIVSDRDLRLDDDTIEALAHGVPAAELGLDREVEAVMSSPVHTIGADEPIDAAARLM
                     LSRRVSALPVLDDAGALAGLLTTTDCLLASLEPAAAR"
     gene            7226..7879
                     /locus_tag="VNU01_06400"
     CDS             7226..7879
                     /locus_tag="VNU01_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH32285.1"
                     /translation="MIRTLLVDDHEVVRKGLRAMLEAQEDIEVVAEAGTAADGVAKAR
                     AIRPDVVVMDVRLPDESGVVACRDIRSALPDTAVLMLTSFSDDQALFDSIMAGAAGYV
                     LKQIKGGELVDGIRRVAAGESLLDPAVTARVLERVRNPQGDEDPRLARLTPTELRILE
                     MIAEGLTNRQIGERIHLAEKTIKNYVSTILSKLQMTRRAEAAAYLADRRATDAARGR"
     gene            complement(7834..8349)
                     /locus_tag="VNU01_06405"
     CDS             complement(7834..8349)
                     /locus_tag="VNU01_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32286.1"
                     /translation="MSRHARVAIRVSHPLAGSAAAVRFGADPASWLPAVVESRGRGRW
                     RLPLSLGGVHRHVALELGDAWRDGADTWRACAWRPQEERGDWIPGPLWLPSFAGSLGV
                     CRRGASAADLVLEGAYRPPFGFAGRAVDRLLLGRLARRSARELLASVAACLAPESYRP
                     RAASVALRSAR"
BASE COUNT         1186 a         3270 c         3065 g         1158 t
ORIGIN      
        1 ggcctggcgt tgcacgaacg tccggctgtc gttgtcgccg gtcttgtcgg acagggcgtc
       61 cagcatcccc cgcaccgtgg ccgccccgac gggatccagg tgtgcggtga gcacgtccat
      121 gccgtcacgc acggaccatt ggagcttgcg gtcctgaaac gcgcggcgtt cccgctccgc
      181 caacacatcc acaccggttt tggcgaccgc ggtgtcgatc tcacgggcca acctcgtccg
      241 gttcgtcgtc tgccccaccg tcgacgcggt ccggtcaatc gtggcgagca gcacctcggc
      301 gtcgtcacgg ccggcgacct cggcggcctt cttgacggcc acggcggcct ggcccaggcc
      361 catgcggccg tcggcgaacg ccgccgccgt gtccggcaac tgcttcagcc cggccgccag
      421 ttcggtgcgc gccttggcct cacccggtgt gaggtgcagc acgcgcgtca gcaggtcctc
      481 ggtggacgcg gcggactggc cgcgggcggc gtggcgctgc tcgatctcga ccaccaccgc
      541 gcacttgtgc gactccgcga ccctgctcac agcttccagg cgctgcacgg cctgggtcaa
      601 cgccacatcg gagaccgcac cgcccagcgg cagcgtcttc gcgcgacgca acgcgcgctc
      661 ggccacgtcc agcagaccgt gaaggtccgc ggtggccagc gccgcgtcga tctcgactcc
      721 cacactcgaa cacatgttcg atagattatc acgagggcgc gactcgtcaa cccacgaaat
      781 tgaatctgtg ggaaagcgcc tgagctgccc agccatgggc acgatgcggg cactgcaact
      841 gcaactccag ccgctgcgcc ctggcgaaat cccccgcccc aggctcccgc gaacgacggt
      901 ggaaggcggg gtacgctagg atcacccgcg ggtgtagctc aatggtagag ccccagcctt
      961 ccaagctggt ggtgccggtt cgatcccggt cacccgctcc acacccatca cggcctgcgt
     1021 cctgccgcgg gtcggctccg ttccgctcac cacgcgccca cgccgtcacg gcgtcgcgca
     1081 tgtcgcgatc cctgggcgcg gcgatcacgg atgggccggc ccagccatgg gactttccac
     1141 gcccaccaac ctcgtgctat acccctgggg ggtttccctg acgtcgctcg gggcggagga
     1201 gaggactgcg tcatggtggg cacgcggacg gcatcggcga cacggcgatc actgactcga
     1261 cgggagcacc tgctgaccgt cggcttcggc ctgtggatgg tggtgggcct gttcctcgac
     1321 ggctgggcgc acgacaacaa ccagcccgag tccttcttca cgccgtggca cgcggtgctg
     1381 tactcgggct tcgtggcggc gggcgccacc gcgctgctga tcgcctggag ggaccgccgt
     1441 cccgggaccc cctgggcgcg cacgctgccc cggggccacg gcctcacgct ggccgggctc
     1501 gcgctgttcg gcgccggcgg cgccggcgac ctggtctggc acgagctgct cggcgtggag
     1561 gtcggcctcg aggcgctgct gtcccccacc cacctgctgc tgctggtcgg gggcctcctg
     1621 gcgctcagcg ccccgttccg cgccgcgtgg agcgagcccg tcacggatcc ggagagcctg
     1681 cgctcgttcg cgccgactgt gctgaccctg gcctttctca ccgcggtgtt cgcgttcttc
     1741 atgctgtact tctcgccgtt cgccaacgac gcggcgggca ccgcgttcga gcgcgtgcac
     1801 ggcgcgtcgc acacgcaccc cagcagcgac ctcggcgagc tccagcagct gctcggcgtc
     1861 gcgtccatcc tcatgaccac cgtgctgctg gtggtccccg cgcacctgct cctgcgccgc
     1921 tgggagccgc ccgccggcac gttcacgctg ttcttcggcg tggtggtgac gctgctgcag
     1981 gccagcggcg agtacgccca gccgagcatg ctgctgctgg gcccgctcgc cggtgcggcg
     2041 acggaccgcc tgctgcgccg cacggcgccg gtcgtgggcg tcgcgggcgg cctcatgctg
     2101 ctgtggctcg gctactttgc gctgtacgcg ctggaggagg gcgcggtggc gtggacggcc
     2161 gagttgtgga ccggcacgac cgtgatggcg gggctgctga ctgcgggcct ggccctgccg
     2221 gcgctgcggg cgccggtacc acccgggacc gacgggccgt agggcgagcg ccaagggcga
     2281 cgctccgaac gcctcccggt caggcggggt gcagcgagcc ctggtgcgcg tcggggaggc
     2341 ggagccgtcc ggcctcgacc tccgtggcga agcggcgcgg gtccatgggg cgagcgaagt
     2401 ggtgcccctg cgcgtcgtcg cagcccagcg agcgcagcac cgcggcctgc tcggcggtct
     2461 ccacgccctc cgcgatggtg cgcaggccga acagggcggc cagcttgacg atcgcctgcg
     2521 ccatggccga ctcctccggc ccgcccacca cgttgcgtac gaacgagcgg tcgatcttga
     2581 gcgcgtccac cggcatctgc cgcaggcccg ccagcgacga gcggccggtg ccgaagtcgc
     2641 cgaccaccac gcgcgcaccc gccgcgcgca gcgcggccag cttgggcacg gccgcgctgt
     2701 gcggcccgag gcagtcctcg cccacctcga ccagcaggca gtcggcggcc aggtcggccg
     2761 cggcgaggtg ctgctcgatc aggccggcca cgtcgccgtg gagctggcga ggcgacacgg
     2821 acagcgacaa cggcacgacc cggcccaggc gctcccgcat ccagcacgcc tgctggaccg
     2881 cctccgtcag gatccactcc cccagcaggc cgatgacccc ggtctcctcg gccaccggca
     2941 cgaacgccga cgccggcagg atgccgtgct gcggatgccg ccaccgcacc agcacctcca
     3001 gcgcgcggac cgtgccgtcc cccatggaca cgacgggctg cacgtgcagg aggaactcgc
     3061 cggcctccac ggcggcgggg acctgggcct ccaggctgag catcgtcgac agggacgact
     3121 gcatgctctc gccataggcg cgccacatgt gcttgccctg ccgcttggcc tcgtacatcg
     3181 cgacgtccgc gcggcgcagc agctcctcgc tctcgatgct gcgctccgcg accaccacgc
     3241 cgatgctggt gcgcaccagc aggtgcgcgc ggtcgacctg caccggctcc tgcagcgcgt
     3301 cgagcacagc ctccgcggca cgcgccgcct ggccggtgtc ggtgttctcc agcagcaccg
     3361 cgaactcgtc gccgccgagc cgggccaccg tgtcgctcgt ccggacgctg tcgcacagcc
     3421 ggtgcgccac ctgcaccagg agcgcgtcgc cggcgctgtg gcccagcgtg tcgttgaccg
     3481 tcttgaagtc gtccaaatcc aggtacagga ccgcgatcga cgcgccggtg cgctcccgca
     3541 ccgccagggc gtgacgcaca cggtccatga acagcgcgcg gttggccaga tcggtcagcg
     3601 ggtcgtggaa cgcccggtac tcgatctcgc gctccagcgc cttgcgctcg gtgatgtcct
     3661 ggacggtgcc catcagccgg gtcgggcggc cgtcgtcgcc caccgccgcg tggcccaggg
     3721 cgtggacgat gcgctcggtc ccgtcggcgc gcaggacccg gcagttgaac tccaggtcgc
     3781 cggccgggac gctcccggcc tgttccacca gcagggcgac gcggccacgg tcgtcctcgt
     3841 gcacgtgcgc gaggaagccc gcgaccgacg gcaggaactc gtccggctgc accccgaaga
     3901 tcgtgtacag ctcccgcgac caccacacgg tgtccgtcgc gatctcccag tcccagctgc
     3961 cgatgctcga cagccgctgc gcgacgagga gatcggcgtt ggaccgctcc agggcgacgc
     4021 gggccgcgcg ctcgccctcc agcgcggtct cgttcaggcg ccacgcggcc aggcacgccg
     4081 cgctctcggc caggacgaac gcgccatgca ccgccgccca gcgccaggcg ccggccagcg
     4141 cggcggggtg gttgaacacc gactccggcg cgagctggcc gacgatgccg tggtgcagga
     4201 ccacgaacgc cagggccacc aggaatggca cccacgactg gtacaggctt gcgatcacca
     4261 ccaccacgaa gaagtggaag tgcatctcga tcacgccgcc ggacaggtgc accgcgatgg
     4321 ccgaggcgct cacgaggccc agggtcgcca tgacggcgcg ggcggcgtcg ggccacaccc
     4381 ggacgctcgc gcccgcggcg agcaccgcca ggatcgccgc ctcacccagg gagtgcaccg
     4441 ccgtctcccc gcgcaccagg gcgaaggcgg ccaccgccgg cacatgggcc cacagcacgc
     4501 cgcagatcgc ccggtgccgc ttgcgccagc tcggcgcgtc caggctgtcg ccggcaggca
     4561 ggaactggcg gagcgcggag atcgcgcgcg ccgggctgcc acccaccttc tcgccgagct
     4621 gagccatcgg gcccccgcaa gcctgcattt ttccgtcctg ggcgatcagc gtgtgtcgat
     4681 gacaaagaga tacgcagaaa ctgcgtacgg tgacgatggg cctttgcccc gatcagtagg
     4741 ggccgaacgt cccgtgcgcc tggcgcacgg aaggggcggc tcttgcagag ccgccccttc
     4801 gcttcttcgg ggtgcgcctc agatgaggta gcggtgccgc tgcaccagcg gcagcttctc
     4861 ccaggcccgg cccaggcccc aggtccggcc ggccccgaac atcgccagcg ccaccaggac
     4921 gatggcgtag acgaggtggt cgtccatgaa cgggttgttg tccagccgca gggacgccgc
     4981 ccacatcgcc gtcatcaaga ggacgccgga cgcggcggcg gcgcgcatcc cgatgcccag
     5041 gatcagcgcc gcgccgatgc cgagcaggcc gagcatgaac gtccagtcgg ccacggggcc
     5101 ggccaggccg gcgaagaagt cgtggaagac cttgccctcc gtgccgaagt tgaggaaccc
     5161 gtaggtcggg gatccgccgt tgacccaggc gttctcggtc gtggtggcga agccgagtcc
     5221 aaaggtcttg tcgaggaacg cccacaggaa cacccagccc aggctgatgc gggcgagggc
     5281 cagggcgtag cgcgcccggt aggcgcggtg ctcttcctcg atccggtccg cgcccatggc
     5341 ggcgagctcc gggtggacgg tctgggtgac gttggcgctc atcttgggat ctcctttgcg
     5401 cgtgtcctgc cgatggggcc agcttgcgcc tggcgccgcc gcctgactgc gggagaacgg
     5461 cccctctcgc gacggcgaac ggcgggggcc cgcgggacgt tcgccgctcc gccgcgggac
     5521 ccccgccggg acttgctcag cgctcgacca ccaccttgag cgcgccggtc tccccggcgc
     5581 gggcgaacac gtcgtaggcg tccaggaact cgcccatgcg gaagtggtgc gtcacgaacg
     5641 gccgtgggtc gacgagctgg gcgccgatga gcctgagcag cgtcggcgtg gagaacgtgt
     5701 ccaccaggcc cgtcgtgatc gtgacgtcac ggatccacag cttctccaag tgcagcgtgg
     5761 ccggctcgcc gtgcacgccg acgttggcga cgcggccgcc cgggcgcacc aggtcggcgc
     5821 acagctcgaa ggtcgccggc acgccgacgg cctcgatcgc cacgtccacg ccgttgcccg
     5881 tcagttccat gacccggtcg atggcgtgct cacggccgct gttgatcgtc gccgtggcgc
     5941 ccatctgctt ggcggcctcc aggcgcgagt cggcgaggtc gatggcgatg atctggctgg
     6001 gcgacagcag cttggccccg gtgatggccg ccagcccgat gggccctgcg ccgaccaccg
     6061 cgacggtctt gccgggtccg accgcgccgt tgagcacgcc gacctcgtag ccggtgggca
     6121 ggatgtcggc gagcatcagc agtgcctcgt cgctcacgcc cgcgggcagc gggtaggtgg
     6181 acgtgtcggc gaacggcacg cgcacccgct cggcctgcgt gccgtcgacc aggtggccga
     6241 ggatccagcc gcctccgccc gtgcactgcc cgtaccggcc ggcgcggcag tactcgcagc
     6301 gcccgcacga ggtgatgcac gacaccagca cgcggtcgcc cggcttcagg gtcttcacgc
     6361 ccgggccgac ctcctcgacg gtgcccacgg cctcgtggcc gaggatgcgg ccctccgcga
     6421 cggtgggcac gtcgcccttg aggatgtgca ggtccgtgcc gcagatcgtg acggcgtcga
     6481 cgcgcacgat cgcatcggtg tcgtcgacga tgcgcgggtc gggcacgtcc tcccagcgct
     6541 tgtcgccggg cccgtggtag acgatggctt tcacagcagg ctccttgtcg ccgtgatcgg
     6601 gtggatctgg cgaccgtcgc accgccgccc caggacgcac agggacgaag cgcccggctg
     6661 tcgtcggcga aggtccccgg cccgccggcc cacctggcac tgccccgccg gggttcacga
     6721 tgcgaggctg ggcggcgtcg agcgagagga gcacggccat gcgcgtgcgc gagtggatga
     6781 gcccggaccc ggtcacggtc accccgtcga cgacggtgcg cgaggccagg cgcctgctgc
     6841 ggcactacgg catccggcac ctgcccgtcc tgggcaggga cggctccctg gccggcatcg
     6901 tcagcgaccg cgacctgcgg ctggacgacg acaccatcga ggcgctggcc cacggcgtgc
     6961 ccgccgccga gctgggcctg gaccgagagg tcgaggcggt gatgtcctcg ccggtgcaca
     7021 ccatcggcgc ggacgagccc atcgacgcgg ccgcgcggct gatgctgtcg cgccgcgtga
     7081 gcgccctgcc ggtgctggac gacgccggcg ccctggccgg cctgctcacc accaccgact
     7141 gcctgctcgc ctcgctggaa ccggcggccg cgcgctgatt gcgcgcgtac gctggcagcg
     7201 tcgccagccg gcccgggagg accacatgat ccgcacgctg ctcgtcgacg accacgaggt
     7261 ggtccgcaag ggcctgcgcg ccatgctcga ggcgcaggag gacatcgagg tggtcgccga
     7321 ggccggcacc gccgccgacg gcgtggccaa ggcgcgcgcg atccggcccg acgtcgtggt
     7381 gatggacgtg cgcctgccgg acgagtccgg cgtggtcgcc tgccgcgaca tccgctcggc
     7441 cctgccggac accgccgtgc tgatgctgac gtcgttctcc gacgaccagg ccctgttcga
     7501 ctcgatcatg gccggcgccg ccggctacgt gctcaagcag atcaagggcg gcgagctcgt
     7561 cgacggcatc cgccgggtgg ccgcgggcga gtcgctgctg gaccccgccg tgacggcgcg
     7621 cgtgctggag cgcgtgcgca acccgcaggg cgacgaggac ccgcgcctgg cgcgcctgac
     7681 gcccacggag ctgcgcatcc tggagatgat cgccgagggg ctgaccaacc ggcagatcgg
     7741 cgagcgcatc cacttggcgg agaagaccat caagaactac gtctccacca tcctgtcgaa
     7801 gctgcagatg acccgtcgcg ccgaggcggc cgcctacctg gccgaccgca gggccacgga
     7861 cgccgcgcgc gggcggtagg actccggggc caggcacgcg gccaccgagg ccagcagctc
     7921 gcgggcgctg cgtcgcgcca acctgcccag gagcagccgg tccaccgcgc ggccggcaaa
     7981 cccgaacggt ggcctgtagg cgccctccag caccaggtca gcggcgctcg cgccgcgccg
     8041 gcacacgccc agcgaaccgg cgaacgacgg cagccaaagc ggtccgggaa tccagtcgcc
     8101 gcgctcctcc tgcggccgcc aggcgcacgc gcgccacgtg tcggcgccgt cgcgccaggc
     8161 gtccccgagt tcgagcgcga cgtggcggtg cacgccgccg agcgagagcg gcaggcgcca
     8221 gcgcccgcgc cctcgggact ccacgacggc gggcagccag ctcgccgggt cggcgccgaa
     8281 gcgcaccgcc gccgccgacc cggcgagcgg gtgggacacc cggatcgcga cacgcgcgtg
     8341 gcgggacatg cggcgtcctc cccgccggcg cgcgtctcgc gccggctccg tgaatcgtgg
     8401 cgccgcggtc cggccggcgg cagggaccaa cggcccggtg cgggcacgcc gatcggcaac
     8461 ccgcgcaggg ccaccacgcc ctgacgaggg ccccccggga cgccgaccct ggtggcacac
     8521 ccaccccgac cccatgcaca aggagcctgg cgatgcgccg ctggatcgtg gagcgcacgt
     8581 tcgtcccggc gtcaccggag cgcctgcgca cgctgctgga ccggcgcatc actccgtcgc
     8641 gtctgccgct gccgggccac ggcgcgcgga cgtccaggc
//
LOCUS       DATMKC010000218         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_335571, whole genome shotgun sequence.
ACCESSION   DATMKC010000218 DATMKC010000000
VERSION     DATMKC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335571"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VNU01_06410"
     CDS             complement(<1..867)
                     /locus_tag="VNU01_06410"
                     /inference="COORDINATES: protein motif:HMM:NF033748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HWH32287.1"
                     /translation="MLAVLAVAVRVLLADAGGAPAALGELVPPLAGGAAAQERAGGDA
                     EHVSLGGPGLAHPLPPLYYDGPREVVPPAAHEPGALAIPELGVESPLLRLDKRPDGRM
                     QVPGDFSLAGWYVRGPAPGQTGPAVIAGHLDSRRGPAVFARLGELAEGDEILVRRGDG
                     HVVRFTVSRVASYPKDAFPTEEVYGNTEGPELRLITCDGDFDRRSRSYAENLVVYAVA
                     DPATLPSDPGTPAPADTPSPAPPTGTPSPEGPAGTPSPESPGPAASPSTSPREDETGL
                     PTEDPTPSPRPSG"
     gene            complement(945..1298)
                     /locus_tag="VNU01_06415"
     CDS             complement(945..1298)
                     /locus_tag="VNU01_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32288.1"
                     /translation="MTKGMPFRTRLALAFAAVLVVSAFAAPVAWAQSPDDLPDFGDLP
                     IPGGEDPAEDPAEDPADPGTSDPGNQVDAPGGGVDTGAGGLADGESALPLAAAGALAA
                     MGGAVLVARRRGLRV"
     gene            complement(1394..>2478)
                     /locus_tag="VNU01_06420"
     CDS             complement(1394..>2478)
                     /locus_tag="VNU01_06420"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32289.1"
                     /translation="GGVRIQGTTGTVTVSGDKFRSTLGLRSTLITEIRQNVAVDPCAG
                     TGAPTSGVSRHAGASRVETAVEVSKANWPQGGSEDVLLATSQNFPDALASASLAAALD
                     APLLLTPTSSLAPAVEGELARLGAKTVWILGGTAAVSAGVEEELGDLGYDTRRVRGGN
                     RFETARAIALAPEARQVGTVALGMDQDWPSAATAGALATLPERIPTLLVHTDAVPEVT
                     ETTLAELGAREVILVGGTGAISADVDQRLRDLGYQVRRLAGDSRYSTSSAVAGEVLAK
                     LPAGQRTLVVATGNDFPDALAAGALAARLDGPMLLSPQCTLGDSPATSDFLTANKARL
                     DKAALVGGRAALSDRVMEQVAAGIGG"
BASE COUNT          300 a          945 c          949 g          284 t
ORIGIN      
        1 gccggagggc ctgggcgagg gcgtggggtc ctcggtcggc aggccggtct cgtcctcgcg
       61 cgggctggtg gaaggcgacg ccgccggacc ggggctctcc ggcgagggcg tgccggcggg
      121 gccctccggc gaaggcgtgc cggtgggggg cgccggcgag ggcgtgtcgg cgggggccgg
      181 tgtgcccggg tcggacggca gcgtcgccgg gtcggcgacc gcgtacacca ccaggttctc
      241 ggcgtagctg cggctgcggc ggtcgaagtc gccgtcgcag gtgatcaggc gcagctccgg
      301 cccctcggtg ttgccgtaca cctcctcggt ggggaacgcg tccttgggat aggaggcgac
      361 acggctcacc gtgaagcgca cgacgtggcc gtccccgcgg cgcaccagga tctcgtcgcc
      421 ttcggccagc tcgcccagcc gcgcgaacac cgccggcccc ctgcgcgagt ccaagtgccc
      481 cgcgatcacg gccggcccgg tctggccggg ggcggggccg cgcacgtacc agccggccag
      541 ggagaagtcg cccggcacct gcatgcgccc gtccgggcgc ttgtccaggc gcagcagcgg
      601 cgactcgacg cccagctcag ggatcgccag cgcaccgggc tcgtgcgcgg cgggcggcac
      661 tacctcgcgc gggccgtcgt agtacagcgg cggcagcggg tgagccagcc ccggcccgcc
      721 gaggctgacg tgctcggcgt cgccgccggc gcgctcctgc gcggccgcac cgccggccag
      781 cggcggcacc agctcgccca gcgccgcggg tgcgccgccg gcatccgcca ggagcacccg
      841 caccgccacg gcaaggaccg cgagcacgag aaaggccccg aaagccgccc cgacgagatc
      901 aggtcggcgc ccggagccct ccacgagatt ccttcggtgg accgctagac ccgcaggccg
      961 cggcggcgcg caacgagcac ggcgccgccc atggcggcca gggcgcccgc cgcggcaagc
     1021 ggcagcgcgc tctcgccgtc ggcgagcccg cccgcgccgg tgtccacgcc gccgcccggg
     1081 gcgtccacct ggttgccggg gtccgaggtg ccggggtccg cggggtcctc ggccgggtcc
     1141 tccgcggggt cctccccgcc cgggatgggc aagtcgccga agtcggggag gtcgtcaggg
     1201 ctctgcgccc aggccaccgg ggcggcgaag gcggacacca ccaggacggc ggcgaacgcc
     1261 agcgccagac gcgtacggaa gggcatgccc ttggtcacga cttcctcctt gaggctcctg
     1321 cgggtccgcg aatcccaccc ccgccgactg cgatgtggcc tcatcgtacg cgaggccgca
     1381 agggcgggcg ggcctagccg ccgatgccgg ccgcgacctg ctccatcacc cggtcgctga
     1441 gcgccgcgcg cccgccgacg agcgccgcct tgtccaggcg cgccttgttg gcggtgagga
     1501 agtcggaggt cgccggcgag tcgcccagcg tgcactgggg cgacagcagc atcggcccgt
     1561 cgagcctggc cgccagcgcg ccggcggcca gcgcgtcggg gaagtcgttg ccggtggcca
     1621 ccaccagggt ccgctgcccg gcgggcagct tggcgagcac ctcgccggcg accgcgctgg
     1681 acgtggagta gcgggagtca ccggccaggc ggcgcacctg gtagcccagg tcgcgcaggc
     1741 gctggtccac gtccgcggag atcgcgcccg taccgccgac caggatcacc tcacgcgcgc
     1801 ccagctcggc cagcgtggtc tcggtcacct ccggcacggc gtcggtgtgc accagcagcg
     1861 tggggatccg ctccggcagg gtggccagtg cgccggccgt ggcggccgac ggccagtcct
     1921 ggtccatgcc cagcgccacg gtgccgacct gtcgcgcctc gggggcgagc gcgatcgcgc
     1981 gcgccgtctc gaagcggttg ccgccacgga cgcgccgggt gtcgtagccc aagtcaccga
     2041 gctcctcctc gacgccggcc gacacggccg cggtaccgcc gaggatccac acggtcttcg
     2101 cgcccaggcg cgccagctcc ccctccacgg ccggcgccag cgacgacgtc ggcgtcagca
     2161 gcagcggcgc gtcgagggcc gccgccagcg acgcggaagc cagcgcgtcg gggaagttct
     2221 gcgaggtcgc cagcagcacg tcctcgctgc cgccctgcgg ccagttggcc ttggacacct
     2281 cgacggcggt ctcgacgcgg ctggccccgg cgtggcgcga cactccgctg gtcggcgccc
     2341 cggttccggc gcacgggtcc accgcgacgt tctggcggat ctcggtgatc agcgtggagc
     2401 gcaagcccaa tgtggagcgg aacttgtcgc cggacacggt gaccgtgccg gtggtgccct
     2461 ggatgcgcac gccgccgt
//
LOCUS       DATMKC010000219         5705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_56268, whole genome shotgun sequence.
ACCESSION   DATMKC010000219 DATMKC010000000
VERSION     DATMKC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56268"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2607
                     /gene="glgP"
                     /locus_tag="VNU01_06425"
     CDS             <1..2607
                     /gene="glgP"
                     /locus_tag="VNU01_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWH32290.1"
                     /translation="DRDHVIKALRTFRVRPSLPPELEPLATLATNLRWAWHPPTHDLF
                     AGIDPALWEEAGENPVGLLGRLSHERLAALAADGAFTERVARLHDDLRAYLEEPRWAQ
                     AQDPVPPRVAYFSPEFGLTHVMQTYSGGLGVLAGDHLKAASDLGLELVAVGLLYRHGY
                     FRQWLDADGWQQEHYPDLNPYGLPLRRLESDGEAVLVEIPMGGHAAFAQIWLAQVGRI
                     PLLLLDTDVAQNAAADRTVTDKLYGGDVEHRVRQELVLGVGGVRALQAAHDQGAIPFL
                     PDLWHSNEGHAGFLQMERIRRLVEHGMGFDEALETARAAVLFTTHTPVPAGIDVFPRE
                     LMERYFGGLAEELGVGMDRLMAVGQPPGSSPDDPFNMAVMGLRLSTAANGVSELHGVV
                     SREMFGHLWPGFDVPEVPITSVTNGVHAPTWVGEEMRRVFDGHLPSDWADAPEAWERV
                     GEIPDEVLWGAREEARARMVAEVRRWVRRQHERRGENPATLGWIDDILDPDTLTIGFA
                     RRFAEYKRGTLLLRQPERLRALLLAQDRPVQFVFAGKAHPRDDLGKDIIRQLVHYSAA
                     EDIRHRIVFVEDYDMDLARVLLAGVDVWLNNPRRPHEACGTSGMKAVLNGGLHCSTLD
                     GWWDEMYDGENGFAIGGHYHTDDAAHQDAADATALFDLLERVIVPRFYDRGAAGSDRP
                     DGGPPPRWMELVRRSLSTLGPRVLATRMVREYSQRLYQPVGEHAARLGQDSGRRGRSL
                     ASWKRHLREGWSGVRVLGLDGDQSPALLGEHREVHARVALGRLRPDDVRVELLHGGVR
                     ADGSLRDPVVTPLRLEGVGSDGAHVYTGGFDAAVSGEYGLTARVVPWHEDLLQWTDAG
                     LVAWATEGESEK"
     gene            2604..4253
                     /locus_tag="VNU01_06430"
     CDS             2604..4253
                     /locus_tag="VNU01_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWH32291.1"
                     /translation="MTDVPAAAWWRHAVGYEVYVRSFADSDGDGVGDLPGLRDRLDHL
                     AWLGVDIVWITPFYPSPMHDHGYDVADYTGVHPEFGSLDDLDAVVARAHELGLRVVID
                     LVPNHTSSAHPWFRAARSSPDSPYRDYYIWRDGRVPGPPEQGGEPPNNWVSVFGGRAW
                     SYDRASGQWWLHLFLPEQPDLNWANPGVRDEFDRILRFWLERGVDGFRIDVAHALAKH
                     PDLPDIPEARRPADPHDIGDAVSQSQWERYEHPYDIDQEEVTEIYRRWRRVVEPHGGL
                     LLGEVYLQDPAKLARYLNPQDGLHLSFWFKPLHIRWSPEAVHQVLRGAIDEVPDHLAW
                     VQGSHDRSRAATRFGGGDLGRARSLVLATLFMGLPGVPFLYMGEELGLEDVEVPPERS
                     QDPIAVRHAAPERARDNCRTPLPWAPVPGLGFTTAEDAWLPFGDRAPADTVAVQREDP
                     SSWLHRWRRLIEARRSFDLTNRHIQWILEEGDVVAYRRGHTIVAANCGDAPGELPLPE
                     GDWEVGFDSASSAAGAPGDRKPALVGTLRLEPLRAVILHRS"
     gene            4284..5702
                     /locus_tag="VNU01_06435"
     CDS             4284..5702
                     /locus_tag="VNU01_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWH32292.1"
                     /translation="MSHRPTAEAAVRLLPERAVPSLEDYVAAGGGAGLRRALEMDPAE
                     VREQVRRSGLRGRGGAGFPTGVKWASVVEAADAGGTVYLVCNAAEGEPGTYKDRVLMR
                     RNPYQMLEGVLIAMHATGAAQAYIPTKERFVEVRERLAEALQAMRATRWEGADRVQLV
                     AGPDAYLFGEESAMLEVIEGRLPMPRILPPYQQGLFADISHANPTVVNNVETLSHVSH
                     ILARGDDWFREVGTVETPGTMVFSVLGDVDSPGVYELPMGTPMRTLIVDIAGARDVKA
                     IYSGVSNTVITPDLLDLPMDFDSFREAGSGLGSGGFVVYDSSRDIVQVLAQLQRFLGI
                     ESCGQCNACKLGNMDMAERLDKFVRGEGTSGDIEQLRKRLATVTDQNRCYLPVGAQLT
                     VGSTLEAFAEEFHAHVGKPADPAVAVMVPKVIRLHPDTGDVTLDDDYWRKRPDWSSVT
                     ERPADAWPGRGAVAAQPELAGD"
BASE COUNT          755 a         2094 c         2096 g          760 t
ORIGIN      
        1 gatcgggatc acgtgatcaa ggcactgcgc acattccgcg tccggccctc gctgccgccg
       61 gagctcgagc ccctggccac gctcgcgacg aacctgcgct gggcgtggca cccgcccacg
      121 cacgacctgt tcgccggcat cgacccggcg ctgtgggaag aggcggggga gaaccccgtc
      181 gggctgctgg gccgcctgtc ccacgagcgc ctcgccgccc tggccgccga cggcgccttc
      241 accgagcgcg tggcgcggct gcacgacgac ctgcgcgcct acctggagga gccccgctgg
      301 gcgcaggcgc aggacccggt gccgccccgc gtggcgtact tctcgcccga gttcggtctc
      361 acccacgtca tgcagaccta ctcggggggc ctcggcgtgc tggccggcga ccacctcaag
      421 gcggccagcg acctgggcct ggagctcgtc gcggtggggc tgctgtaccg ccacggctac
      481 ttccgccagt ggctggacgc agacggctgg cagcaggagc actacccgga cctcaacccc
      541 tacggcctgc cgctgcggcg cctggagtcc gacggcgagg ccgtcctggt cgagatcccg
      601 atgggcggcc acgccgcgtt cgcgcagatc tggctggccc aggtgggccg gatccccctg
      661 ctgctgctgg acaccgacgt ggcgcagaac gccgcggcgg accgcacggt cacggacaag
      721 ctctacggcg gcgacgtgga gcaccgcgtc cgccaggagc tggtgctggg cgtcggcggc
      781 gtgcgcgcgc tccaggcggc gcacgaccag ggcgcgatcc cgttcctgcc ggacctgtgg
      841 cactccaacg agggccacgc cggcttcctg cagatggagc gcatccggcg cctggtcgag
      901 cacggcatgg gcttcgacga ggcgctcgag accgcccggg ccgccgtgct gttcaccacg
      961 cacacgccgg tgcccgccgg catcgacgtg ttcccgcgcg agctcatgga gcgctacttc
     1021 ggcgggctcg ccgaggagct cggcgtcggc atggaccggc tgatggccgt gggccagccg
     1081 ccggggtcca gccccgacga cccgttcaac atggccgtca tgggcctgcg cctgtccacc
     1141 gccgccaacg gggtgtcgga gctgcacggc gtggtgtccc gcgagatgtt cggtcatctg
     1201 tggcccgggt tcgacgtgcc ggaggtgccg atcacgtccg tgaccaacgg cgtgcacgcg
     1261 cccacctggg tgggcgagga gatgcgccgg gtgttcgacg ggcacctgcc gtcggactgg
     1321 gcggacgcgc cggaggcgtg ggagcgcgtc ggggagatcc ccgacgaggt gctgtggggc
     1381 gcgcgcgagg aggcgcgggc ccgcatggtc gcggaggtgc gccgctgggt gcgccgccag
     1441 cacgagcgcc ggggcgagaa ccccgccacg ctcggctgga tcgacgacat cctggacccg
     1501 gacacgctca ccatcggctt cgcccgccgc ttcgccgagt acaagcgcgg cacgctgctg
     1561 ctgcgccagc ccgagcggct ccgggcgctc ctgctggcgc aggaccgccc ggtccagttc
     1621 gtcttcgccg gcaaggcgca tccgcgcgac gacctgggca aggacatcat ccgccagctc
     1681 gtgcactaca gcgcggccga ggacatccgc catcgcatcg tgttcgtcga ggactacgac
     1741 atggacctcg cccgcgtgct gctggcgggc gtggacgtct ggctgaacaa cccgcgtcgg
     1801 ccgcacgagg cgtgcggcac gtccggcatg aaggcggtgc tcaacggcgg cctgcactgc
     1861 tcgacgctgg acggctggtg ggacgagatg tacgacggcg agaacggctt cgccatcggc
     1921 gggcactacc acaccgacga cgcggcccat caggacgccg ccgacgccac ggcgctgttc
     1981 gacctgctgg agcgcgtcat cgtgccgcgc ttctacgacc gcggggccgc gggctcggac
     2041 cggcccgacg gcggcccgcc gccgcgctgg atggaactgg tgcgccgctc cctgtccacg
     2101 ctgggcccgc gcgtgctggc cacgcgcatg gtgcgtgagt acagccagcg cctgtaccag
     2161 ccggtggggg agcacgccgc ccgcctggga caggactccg gccggcgcgg ccggtcgctg
     2221 gcgtcgtgga agcgccacct gcgcgagggc tggagcggcg tgcgcgtgct cggcctggat
     2281 ggcgaccagt cgccggcgct gctgggcgag caccgcgagg tgcacgcccg cgtggcgctc
     2341 gggcgcctgc ggccggacga cgtgcgcgtg gagctgctgc acggcggcgt gcgcgcggac
     2401 ggcagcctgc gcgacccggt ggtgacgccg ctgcgcctgg agggcgtcgg gtcggacggc
     2461 gcgcacgtct acacgggcgg cttcgacgcc gcggtgtccg gggagtacgg cctgaccgcc
     2521 agggtggtgc cctggcacga ggacctgctg cagtggaccg acgccggact ggtcgcctgg
     2581 gcgacggagg gtgagagcga gaagtgacgg atgtgccggc cgcggcatgg tggcgccacg
     2641 ccgtcggcta cgaggtgtac gtgcgctcct tcgccgactc cgacggcgac ggggtgggcg
     2701 acctgccggg cctgcgcgac cggctggacc acctggcgtg gctgggcgtg gacatcgtct
     2761 ggatcacgcc gttctacccc tcgcccatgc acgaccacgg ctacgacgtg gccgactaca
     2821 ccggggtgca cccggagttc ggctcgctgg acgacctgga cgccgtggtc gcccgggcgc
     2881 acgagctggg cctgcgcgtg gtcatcgacc tggtgcccaa ccacacgtcg tcggcgcatc
     2941 cgtggttccg cgccgcgcgc tcgtcgccgg acagccccta ccgggactac tacatctggc
     3001 gcgacggccg cgtcccgggc cccccggagc aaggcggcga gccgccgaac aactgggtca
     3061 gcgtgttcgg cggccgcgcg tggtcctacg accgggcctc cgggcagtgg tggctgcacc
     3121 tgttcctgcc cgagcagccg gacctgaact gggccaaccc cggcgtgcgc gacgagttcg
     3181 accggatcct gcgcttctgg ctggagcgcg gcgtcgacgg cttccgcatc gacgtggccc
     3241 acgcgctggc caagcacccc gacctgcccg acatcccgga ggcgcggcgt cccgccgacc
     3301 cgcacgacat cggcgacgcg gtgtcgcagt cgcagtggga gcgctacgag cacccctacg
     3361 acatcgacca ggaggaggtc accgagatct accggcgctg gcggcgggtg gtcgagcccc
     3421 acggcggcct gctgctcggc gaggtgtacc tgcaggatcc cgccaagctc gcgcgctacc
     3481 tcaacccgca ggacgggctg cacctgtcgt tctggttcaa gccgctgcac atccgctgga
     3541 gccccgaggc cgtccaccag gtcctgcgcg gcgcgatcga cgaggtgccc gaccacctcg
     3601 cgtgggtcca gggcagccac gaccgttcgc gcgccgcgac ccgcttcggc ggcggcgacc
     3661 tgggccgggc ccgctcgctc gtgctggcga cgctgttcat gggcctgccg ggcgtgccgt
     3721 tcctgtacat gggcgaggag ctgggcctgg aggacgtgga ggtgccgccc gagcgctccc
     3781 aggaccccat cgccgtgcgg cacgccgcgc cggagcgcgc gcgcgacaac tgccgcaccc
     3841 cgctgccctg ggcgcccgtg cccgggctgg gcttcaccac cgccgaggac gcctggctgc
     3901 ccttcggcga ccgcgccccg gccgacaccg tggcggtgca gcgggaggac ccttcgagct
     3961 ggctgcaccg ctggcggcgg ctcatcgagg cgcgccgcag cttcgacctg acgaaccggc
     4021 acatccagtg gattttggaa gagggggacg tggtggccta ccgtcgcggc cacacgatcg
     4081 tcgcggccaa ctgcggcgac gcgcccggcg agctcccgct tcccgaaggt gactgggagg
     4141 tgggcttcga ctccgcgtcc tccgccgccg gcgcccccgg tgatcggaag cccgcgctcg
     4201 tgggcaccct gcgactggag cccctgcgcg cggtgatcct gcaccggtcc tgacgcaccg
     4261 gccctgagaa ggagtccgcc cccatgtccc accgccccac cgccgaggcc gccgtccggc
     4321 tgctgcccga gcgcgccgtg ccctcactgg aggactacgt cgccgcgggg ggcggcgcgg
     4381 gactgcgccg cgcgctggag atggaccccg cggaggtccg cgagcaggtg cgccgctccg
     4441 gcctgcgcgg ccgcggcggc gcgggcttcc ccaccggcgt gaagtgggcc tccgtggtgg
     4501 aggcggcgga cgccggcggc acggtgtacc tggtgtgcaa cgccgcggag ggcgagcccg
     4561 gcacgtacaa ggaccgcgtg ctcatgcggc gcaaccccta tcagatgctg gagggcgtgc
     4621 tgatcgcgat gcatgcgacc ggcgcggcgc aggcctacat ccccaccaag gagcgcttcg
     4681 tggaggtgcg cgagcgcctg gccgaggcgc tccaggccat gcgcgccacc cgctgggagg
     4741 gggccgatcg cgtgcagctc gtggccggcc ccgacgccta cctgttcggc gaggagtcgg
     4801 cgatgctcga ggtcatcgag ggccgcctgc cgatgccgcg catcctgccg ccctaccagc
     4861 agggcctgtt cgccgacatc tcccacgcca acccgaccgt ggtgaacaac gtggagacgc
     4921 tgtcccacgt cagccacatc ctggcccgcg gcgacgactg gttccgcgag gtcggcaccg
     4981 tcgagacgcc cggcaccatg gtgttctccg tgctcggcga cgtggacagc cccggggtct
     5041 acgagctgcc catgggcacg cccatgcgca cgctgatcgt cgacatcgcc ggcgcccggg
     5101 acgtgaaggc gatctacagc ggcgtgtcca acaccgtcat caccccggac ttgctggatc
     5161 tgccgatgga cttcgacagc ttccgcgagg ccggcagcgg actgggttcc ggcggcttcg
     5221 tcgtgtacga ctccagccgg gacatcgtgc aggtgctcgc ccagctccag cggttcctgg
     5281 gcatcgagtc ctgcgggcag tgcaacgcct gcaagctcgg caacatggac atggccgagc
     5341 gcctggacaa gttcgttcgt ggcgagggaa cgtccgggga catcgagcag ctgcgcaagc
     5401 gcctggccac ggtgaccgac cagaaccgct gctacctgcc ggtgggcgcg cagctcaccg
     5461 tgggcagcac gctggaggcc ttcgccgagg agttccacgc ccacgtgggc aagccggcgg
     5521 acccggccgt ggccgtcatg gtgccgaagg tcatccgcct gcacccggac accggcgacg
     5581 tgacgctgga cgacgactac tggcgcaagc ggccggactg gtcctccgtc accgagcgtc
     5641 cagccgacgc ctggccgggc cgcggcgccg tggccgccca gccggagctc gccggcgact
     5701 gaaac
//
LOCUS       DATMKC010000220         4878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_157193, whole genome shotgun sequence.
ACCESSION   DATMKC010000220 DATMKC010000000
VERSION     DATMKC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157193"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VNU01_06440"
     CDS             <1..275
                     /locus_tag="VNU01_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWH32293.1"
                     /translation="LVLCYLLLVGRGFAVALRARDDFGMLLATGLTAIFALQVFVILG
                     GVTRLIPLTGLTLPFASYGGSSLLANYVLLALLLRVSSGPVRESGA"
     gene            272..1762
                     /locus_tag="VNU01_06445"
     CDS             272..1762
                     /locus_tag="VNU01_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWH32294.1"
                     /translation="MSRQVRRVAWAMFALFGVLFANLNVVQVLKADELAADNRNARQL
                     IREYDIRRGSIVVEASGESESAEIARVVETDGRLRYLRRYADGPLYAHVTGYYSIVYG
                     RAQLEAAANDFLVGSAPETFARNLADLLAGRQRRGDDVITTLDPAVQAAAREALGDRV
                     GAVAALNPTTGDVLALWSNPTYDPNELSSHDPEAIRRTWERLDGDPEQPLLNRATQAW
                     YPPGSTFKVVTAAAALESGMTPAEEFADPVRQPLPQTNAGIGNFGGGTCNGGDPIRLD
                     RALHVSCNTTFAQLGLRLGAEALVAQAERFGLNTSVDFDLPLLDSRIPADLNPPQTAQ
                     SAIGQFEVRVTPLQMALIAGAIANEGTLMRPRVIRRVEDFAGRIMADYEPQPLVLPGR
                     ADAQVMTPENARTLRDMMVGVVASGTGRRARIPGVEVGGKTGTAQHGEGPPFAWFIGF
                     APAVSPQVAVAVVVEGGGGLGDEATGGALAAPVARAVMQAALQGQN"
     gene            1791..3626
                     /gene="pknB"
                     /locus_tag="VNU01_06450"
     CDS             1791..3626
                     /gene="pknB"
                     /locus_tag="VNU01_06450"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HWH32295.1"
                     /translation="MDETTPLTGTRDPRRVLAGRYVLQGLLGQGGMADVELAYDQLLD
                     RQVAVKILHQRYMHDQQFVARFRREAQAAASLNHPNVVAVYDTGEHEGRPYIVMEYVS
                     GRSLKEVLAAGGVMPQRAAEVAGEAALALHYAHERNLVHRDVKPGNIMVSDEGAVKVT
                     DFGIARAVGAESVTQTAAVFGTAAYVSPEQAQGEEVDRRTDVYALGCVLYEMLTGRQP
                     FAADSAVALAYKHVSEDPVPPTHLNPDISATLEAVTIKAMAKDPRQRYQTARDLHDDL
                     QRALRGMAVSAPPVVVFERTQPVGAGQRTAVLPAAGYEEPYAADWYPDEPARRSNASW
                     AVITLLVLAGLAAGVFLLSRLFFGGTEQMATIPPQVVGMDIAEAERLLKELGFDTALG
                     ERQANAEFGPNEVITTDPPPGQTAPLGSTITLIYSEGPPIAEVPDLRGKTEEEAREIL
                     LKAGFGIGTRFTEASDEVDADRIIRSDPEAGETVEAGTPVDIYVSSGPATFEVPSVVN
                     MREAEAIRTLENACDEPPCVRVVTSREFSDSVEEGRVIRQTPEAGSDVEPGSTVTIVV
                     SRGPEEEPTPTQTPTPTQSPSPTPTITEPAPTPTPTPTGGDGLGF"
     gene            complement(3697..4281)
                     /locus_tag="VNU01_06455"
     CDS             complement(3697..4281)
                     /locus_tag="VNU01_06455"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWH32296.1"
                     /translation="MARIFVVDNYDSFTYNLVQELGELGAELEVARHDAFTLDELDAL
                     RPDGIVISPGPGDPDDAGLSNDVIRAFSGRIPILGVCLGMQCLAAVHGAAIVRAPELV
                     HGKTSLIHHDGKGVFAGLDNPFEATRYHSLIVDEATVPDVLEITARTANGLIMGLRHR
                     ELPVEAVQFHPESVLTTSGMALLGNFLQSLPVPA"
     gene            complement(4371..>4878)
                     /locus_tag="VNU01_06460"
     CDS             complement(4371..>4878)
                     /locus_tag="VNU01_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWH32297.1"
                     /translation="TKPVEPGQPGELAIWGPQVMRGYWQRPDATADMIKDGWLLTGDI
                     AQIDEEGYFSIVDRKKDMIIAGGFNIYPRDIEEVLYEHPKVEKAVVAGIPDPYRGETV
                     KAYIVLKEGQRATPEEMDAWCREKLAAYKVPKEYEFRAELPETMVGKVLRRLLIDEEL
                     AKQKAAKN"
BASE COUNT          706 a         1754 c         1743 g          675 t
ORIGIN      
        1 ccctggtgct gtgctacctg ttgctggtcg gccgcggctt cgcggtggcg ctgcgggcgc
       61 gcgacgactt cgggatgctc ctggcgaccg ggctgaccgc gatcttcgcg ctgcaggtgt
      121 tcgtgatcct gggcggggtg acgcggctca tcccgctgac gggcctgacg ctgccgttcg
      181 cgtcctacgg cggctcgtcg ctgctggcca actacgtgct gctggcgctg ctgctgcgcg
      241 tgtcctccgg gccggtgcgg gagtccggcg cgtgagccgc caggtccggc gcgtggcgtg
      301 ggcgatgttc gccctgttcg gggtgctgtt cgcgaacctc aacgtggtgc aggtgctcaa
      361 ggcggacgag ctcgcggcgg acaaccgcaa cgcacgccag ctcatccgcg agtacgacat
      421 ccgccgcggc tccatcgtgg tggaggcgtc cggcgagtcg gagtcggcgg agatcgcccg
      481 cgtggtcgag accgacgggc gcctgcgcta cctgcggcgc tacgccgacg gcccgctgta
      541 cgcccacgtc accggctact actcgatcgt ctacgggcgc gcgcagctgg aggccgcggc
      601 caacgacttc ctggtgggca gcgcgccgga gacgttcgcg cgcaacctgg ccgacctgct
      661 ggcgggccgc cagcggcgcg gcgacgacgt catcacgacg ctggaccccg cggtgcaggc
      721 ggcggcccgc gaggcgctcg gcgaccgggt cggcgcggtc gccgccctca accccacgac
      781 cggggacgtc ctggcgctgt ggtccaaccc cacctacgac cccaacgagc tgtccagcca
      841 cgacccggag gccatacggc gcacctggga gcgcctggac ggcgacccgg agcagccgct
      901 gctgaaccgc gcgacgcagg cgtggtaccc gcccggctcc acgttcaagg tggtgaccgc
      961 cgccgccgcg ctggaaagcg gcatgacgcc ggccgaggag ttcgccgacc cggtgcgcca
     1021 gcccctgccg cagaccaacg cgggcatcgg caacttcggc ggggggacgt gcaacggcgg
     1081 cgaccccatc cgcctggacc gcgcgctgca cgtgtcctgc aacacgacgt tcgcccagct
     1141 ggggctgcgg ctgggcgccg aggcgctggt ggcgcaggcg gagcgcttcg ggctgaacac
     1201 gtccgtggac ttcgacctgc cgctgctgga cagccgcatc ccggccgacc tcaacccgcc
     1261 acagaccgcg cagagcgcca tcgggcagtt cgaagtgcgg gtcacgccgc tgcagatggc
     1321 gctgatcgcc ggcgccatcg ccaacgaggg cacgctcatg cggccacgcg tcatccgccg
     1381 ggtcgaggac ttcgcaggcc gtatcatggc tgactacgag ccccaacccc tggtccttcc
     1441 gggaagggcc gatgcccagg tgatgacccc ggagaacgcc cgcacgctgc gcgacatgat
     1501 ggtcggcgtg gtcgcctcgg ggacgggtcg gcgggcgcgc atccccggtg tcgaggtcgg
     1561 cggcaagacc gggacggccc agcacggcga aggcccgccc ttcgcctggt tcatcggctt
     1621 cgcccccgcc gtctcgcccc aggtggcggt cgccgtggtg gtcgagggcg gtggcggcct
     1681 gggcgacgag gccaccggcg gcgcgctggc cgcaccggtg gcgcgggcgg tcatgcaggc
     1741 cgccctgcaa gggcagaact gacgagcagc cgcaggagga gcacgacggc atggacgaga
     1801 cgacaccgct gacgggcacg cgcgatccac ggcgcgtgct cgccggtcgc tacgtcctgc
     1861 agggcctgct gggccagggc ggcatggccg acgtggagct ggcctacgac cagctcctgg
     1921 accgccaggt ggccgtgaag atcctccacc agcgctacat gcacgaccag cagttcgtgg
     1981 cccgattccg ccgcgaggcg caggctgccg caagcctcaa ccaccccaac gtcgtggccg
     2041 tgtacgacac cggcgagcac gaggggcggc cctacatcgt catggagtac gtctccgggc
     2101 gctcgctcaa ggaggtgctg gcggccggcg gcgtgatgcc gcagcgcgcc gccgaggtgg
     2161 ccggcgaggc ggcgctggcg ctgcactacg cccacgagcg caacctggtc caccgcgacg
     2221 tcaagccggg caacatcatg gtctccgacg agggggcggt gaaggtcacc gacttcggca
     2281 tcgcgcgcgc cgtgggcgcc gagtcggtga cgcagaccgc ggcggtgttc ggcaccgccg
     2341 cgtacgtctc cccggagcag gcgcagggcg aggaggtcga ccgccgcacc gacgtgtacg
     2401 cgctgggctg cgtgctgtac gagatgctga ccggccgcca gccgttcgcc gccgactccg
     2461 cggtggcgct ggcctacaag cacgtctccg aggacccggt cccgccgacg cacctgaacc
     2521 cggacatcag cgccaccttg gaggccgtca ccatcaaggc gatggccaag gacccgcgcc
     2581 agcgctacca gaccgctcgc gacctccacg acgacctcca gcgcgcgctg cgcggcatgg
     2641 ccgtgtccgc gccgccggtg gtggtcttcg agcgcacgca gccggtggga gcgggccagc
     2701 gcaccgcggt gctgcccgcg gccggctacg aggagcccta cgccgccgac tggtacccgg
     2761 acgagccggc ccgccgcagc aacgcgagct gggcggtcat caccctgctg gtcctggcgg
     2821 gtctggccgc gggggtcttc ctgctgtcgc ggctcttctt cggcggcacc gagcagatgg
     2881 ccacgatccc gccgcaggtg gtgggcatgg acatcgccga ggcggagcgg ctgctgaagg
     2941 agctgggctt cgacaccgcg ctcggcgagc gccaggccaa cgccgagttc gggcccaacg
     3001 aggtcatcac caccgacccc ccgcccggcc agacggcacc gctggggagc accatcacgc
     3061 tgatctacag cgaggggccg cccatcgcgg aagtcccgga cctgcgcggc aagacggaag
     3121 aggaggcccg cgagatcctg ctgaaggcgg gcttcggcat cggcacgcgc ttcaccgagg
     3181 ccagcgacga ggtcgatgcc gaccgcatca tccgcagcga tcccgaggcc ggcgagaccg
     3241 tcgaggccgg cacgccggtg gacatctacg tgtcgtcggg gccggccacc ttcgaggtcc
     3301 ccagcgtggt gaacatgcgc gaggccgagg cgatccgcac gctggagaac gcctgcgacg
     3361 agccgccgtg cgtgcgcgtg gtgacgagcc gggagttcag cgactcggtg gaggagggcc
     3421 gcgtcatccg ccagacgccc gaggcgggaa gcgacgtgga gcccggctcc acggtgacga
     3481 tcgtggtgtc gcgaggcccc gaggaggagc ccacgcccac acagacgccg acgccgacgc
     3541 aatctccgtc accgacgccc acgatcaccg agcccgcccc gacgccgacg ccgacgccga
     3601 ccggaggcga cggcctcggc ttctgacccc cgcccaattc ccccgagttg gaagatcagg
     3661 cccgtatgtg ggccagatcc tccaaccttc tcacgctcag gcgggaaccg gcagggactg
     3721 caggaagttg cccagcaagg ccatgccgct ggtggtgagc accgactccg ggtggaactg
     3781 gacggcctcg acgggcagct cccggtggcg caggcccatg atgagcccgt tcgccgtgcg
     3841 ggcggtgatc tccaggacgt ccggcaccgt cgcctcgtcc acgatcaggg agtggtagcg
     3901 ggtcgcctcg aacgggttgt ccagccccgc gaacacgccc ttgccgtcgt ggtggatcag
     3961 cgaggtcttg ccgtgcacga gctctggcgc gcgcacgatc gcggcgccgt gcaccgcggc
     4021 caggcattgc atgcccaggc acacgcccag gatcgggatg cgcccggaga aggcacggat
     4081 gacgtcgttg gacagcccgg cgtcgtcggg gtcccccggg ccgggcgaga tcacgatgcc
     4141 gtccggccgc agcgcgtcca gctcgtcgag cgtgaacgcg tcatgccgcg ccacctcgag
     4201 ctccgccccc agctccccca actcctgcac gaggttgtag gtaaacgagt cgtagttgtc
     4261 caccacgaag atccgcgcca tgcggccaag atagcgccca tgcaaggcgg ggcctgctcc
     4321 gaccgagaga gcctctttat cggtctccgc aggcccgcct tgctgggcgc tcagttcttg
     4381 gccgccttct gctttgcgag ttcctcgtcg atgaggaggc ggcggaggac cttgccgacc
     4441 atggtctccg gaagctcggc gcggaactcg tactccttcg gcaccttgta cgccgccagc
     4501 ttctcgcggc accaggcgtc catctcctcg ggggtggccc gctggccctc cttgagcacg
     4561 atgtaggcct tcacggtctc gccgcggtag gggtcgggga tgccggcgac gaccgccttt
     4621 tccaccttgg ggtgctcgta gagcacctct tcgatgtcgc gcgggtagat gttgaacccg
     4681 cccgcgatga tcatgtcctt cttgcggtcg acgatggaga agtagccctc ctcgtcgatc
     4741 tgcgcgatgt cgccggtgag cagccagccg tccttgatca tgtcggcggt ggcgtccggg
     4801 cgctgccagt agccgcgcat gacctgcggc ccccagatcg cgagctcgcc gggctggccc
     4861 ggctcgaccg gcttggtg
//
LOCUS       DATMKC010000221         7361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_68670, whole genome shotgun sequence.
ACCESSION   DATMKC010000221 DATMKC010000000
VERSION     DATMKC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68670"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2694
                     /locus_tag="VNU01_06465"
     CDS             <1..2694
                     /locus_tag="VNU01_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWH32298.1"
                     /translation="IQLLADSHGHCIHLGERDCSVQRRHQKLVEIAPAPGLPDALRER
                     LAADAVRIASEAGLTGLATVEFLVDADALAAGRSGDAHVFMEVNPRLQVEHTVTEEVL
                     GLDLVALQLRVADGATLADLGVAGGVAPRGAAVQARVNAEAIAPGGAVTPTAGTVTAL
                     ALPGGPGVRVDTHAEAGMEVSGRYDTLLAKVIAHAPGGTADAAVRLSAALAELRVDGV
                     ATNAALLRALLEQPEVARGEATTTLLDERLPALLERAGALAAGSGTGRGSAAADTAPA
                     PTTGDVQEAEGQLVVRAPMLGTVARVPVLAGDTVAADAPLVLIEAMKMEHVVAAGSAG
                     TVRRILVRPGQEVGAGAPLAVVEPAGAGVAATAAQVAVDPDAERPELAESRRRHWLLT
                     DEARPEAVAKRHALGRRTARENLDDLVEPGSFVEYGGLAVAAQRARRDLGDLIQSTPA
                     DGLIGGVGRVNAELFPDDRAQAVVLSYDYMVLAGTQGVFGHRKKDRLFELTERLRRPV
                     VFFTEGGGGRPGDTDQAMVAGLDTVAFALWAKLSGVVPRIGVASGRCFAGNAALYGMC
                     DVTIATREASIGMGGPAMIEGGGLGVVDPDDVGPLSVQVPNGVVDIAVDDDAAAVAMA
                     KRVLSYFQGAVTAWEAGDQRLLRTLVPEDRKRAYRVRPVIDALCDAGSVTELREAFGP
                     GMVTALARLEGRPVGIVANDPVHMAGAITSDGADKAARFLQLCEAFGLPVLFLCDTPG
                     IMVGPEAEATGLVRHAARLFLAGGALTVPFVTVVLRKGYGLGAQAMAGGSFRSPLATL
                     SWPTGEFGGMGLEGAVKLALRKELAAIEDPAERQAAFDNAVAWAYEQGKATSMATYFE
                     IDDVIDPADTRHVVLQLLRSAPPHASPDASHARRPRYVDAF"
     gene            complement(2710..5214)
                     /locus_tag="VNU01_06470"
     CDS             complement(2710..5214)
                     /locus_tag="VNU01_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32299.1"
                     /translation="MRQRRPVWMIPLAAMLGVALLAAAPSGAVVPVVEPDPATPAAQI
                     IQALADTTGPQQPPADPPQGREPGDRYDLAGGCWAVQSAASGGFVRRDGGGFAATADA
                     AGAEPFHFQATDLGRYLLYGSAQDFVAASDSPLGTAADAATGSGPGGIAEGASIGATG
                     VAARAVGDGAAADATGRGAAVVSAAAPSELADWDVDEHGNGFRITLPAAGKALAAGGD
                     GRLVLVDAADKGKATRFAFTRTQGCATFPEVEIGVDGPVAIGASPTGEVRGFLDAHLH
                     MMAFEFLGGRARCGRPWHRYGVTYALVDCPDHEPGGAGAVLENVLAGGDVPVHDTVGW
                     PTFGYWPRYDSLTHEQVYYKWLERSWRGGLRMFVNLLVDNNVLCEIYPYKSHGCNEMD
                     GVRLQAQRIRELERYIDAQSGGPGEGWFRIVTDPFEARRVMNAGKLAVVLGIEVSVLF
                     DCGVFAEQARCDHAQIDKQLDEVHALGVRQMEFVNKFDNALAGVAGDAGSTGIVVNNG
                     NFIETGKFWQMETCTEADGHAHDRQQHNFHDDSGSPDELTGRDALAGGILRLTGTSGA
                     APVYPEGPHCNVRGLTHLGEYLIRKMVQKGMIFDPDHMSARARTQAMDIVEELGYPGV
                     VSSHGWADETTYPRIYRQGGVVTPYGGSSTSFVANWRQHKQWADPRWYWGFGYGADTN
                     GFGSQGAPRGAGAENKVTYPFTGFGGVTVHRQRSGERVYDINTDGVAHYGLYPDWIED
                     LRKLAGDEIVEDLARGPEAYLQMWERALGIAPDGCAAAQTAGRADSRVRRGMTPEQVL
                     LALGQPEQRGARSFSYCAGKVVVAFDDTGRVSAVRR"
     gene            complement(5310..>7361)
                     /locus_tag="VNU01_06475"
     CDS             complement(5310..>7361)
                     /locus_tag="VNU01_06475"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWH32300.1"
                     /translation="ARRLAEPLQPMAAIPEYLAELTVAFDAHAAELVLRVDGGREIHR
                     HDRDRGGCGSRLEDENVASLEGVLAASPGAVAVTADDGDAVSRALRAAGAKDCLAAPL
                     MDGDRLMGAVLVLDRGGFAGSRSGELAIFEALARETAGALAKGRLLGDVLEERRKLAE
                     IVDSASDGICTFSQDGTVLTWNPALEHITGLPAESVVGTRDIGRRLRVRTAAGEQVDL
                     RRGSLESLPRDIRLMAMDGRRRHLTCSYSAGSMEQGGAALIVVARDVTPAEEHEALRA
                     QFSQLLEADAARRVVVEQLQQTVVPAPIAVKGAELAAAYESSDPKAPTGGDLYDWQVL
                     PSGEVHVAVVDVLGHGVAATKDALAVVHTLRVLTASGTPLELLVSRADELLQAQHPEL
                     VATVIVARYAPDTGRLRVAAGGHPPALVVTPRREVTQVAASGGVIGWPGAGSDTISET
                     VLEPGDALVMYTDGLVEARKNILDGMDDLVRHAAEVADLPADEFARELVSRALAGAER
                     RDDTLALVLRRDPVHAEDQRASWEASPDPAEISQTRRALRDWLSRRGVDAHDASLAAG
                     ELLSNAMRSARGRVVLGVCLTDAAIVVDVSDDGQGALDLDQRGRALPPADTEAGRGLY
                     IVRAVAQRVDVLSTSEGSTIRAVLSRHSPLPPRSRADVQDGASLDARALLADEWTWAQ
                     P"
BASE COUNT          891 a         2712 c         2818 g          940 t
ORIGIN      
        1 atccagctgc tggccgacag tcacgggcac tgcatccacc tgggcgagcg cgactgctcc
       61 gtgcagcggc gccaccagaa gctggtggag atcgcgccgg cgccgggcct gccggacgcg
      121 ctgcgcgagc ggctggccgc cgacgccgtg cgcatcgcct ccgaggcagg actgacgggg
      181 ctggcgaccg tggagttcct ggtggacgcc gacgcgctgg cggcgggccg atccggtgac
      241 gcgcacgtgt tcatggaggt caacccgcgg ctgcaggtgg agcacaccgt caccgaggag
      301 gtgctcggcc tggacctggt ggcgctgcag ctccgcgtgg cggacggcgc caccttggcc
      361 gaccttggcg tcgcgggcgg tgtggcgccg cgcggcgccg ccgtgcaggc gcgcgtgaac
      421 gccgaggcca tcgcgcccgg cggcgccgtg acaccgaccg cggggacggt gaccgcgttg
      481 gcgctgcccg gcggcccggg cgtccgcgtc gacacccacg ccgaggccgg catggaggtg
      541 agcggccgct acgacaccct gctggccaag gtgatcgcgc acgcacccgg cgggaccgcc
      601 gacgcggccg tgcggctgtc cgccgcgctg gccgagctgc gcgtggacgg cgtggccacg
      661 aacgccgcgc tgctgcgcgc gctgctggag cagccggagg tcgcgcgcgg tgaggccacg
      721 accacgctgc tcgacgagcg gctgcccgcc ctgctggagc gcgccggcgc cctggcggcc
      781 ggctccggga ccgggcgggg cagcgccgcg gcggacaccg cccccgcccc gaccaccggc
      841 gacgtgcagg aggcggaagg gcagctggtc gtgcgcgcgc ccatgctggg caccgtggcg
      901 cgcgtgccgg tgctggccgg cgacaccgtg gccgcggacg cgccgctggt gctcatcgag
      961 gcgatgaaga tggagcacgt ggtggccgcc ggcagcgccg ggaccgtgcg ccgcatcctc
     1021 gtgcgccccg gccaggaagt gggggcgggc gcgcccctgg ccgtcgtcga gccggcgggc
     1081 gccggcgtcg cggccaccgc ggcgcaggtg gcggtggacc ccgacgccga gcgccccgag
     1141 ctggcggaga gccggcggcg gcactggctg ctcaccgacg aggcgcggcc cgaggcggtg
     1201 gccaagcgcc acgcgctggg gcggcgcacg gcgcgggaga acctggacga tctggtggag
     1261 ccgggcagtt tcgtggagta cggcgggctg gccgtcgcgg cgcagcgcgc gcgccgcgac
     1321 ctgggggacc tcatccagtc cacgccggcc gacggcctga tcggcggggt gggccgggtc
     1381 aacgccgagc tcttccccga cgaccgcgcg caggccgtgg tgctgtccta cgactacatg
     1441 gtgctggccg gcacccaggg cgtgttcggc caccgcaaga aggaccggct gttcgagctc
     1501 accgagcggc tgcgccggcc ggtggtgttc ttcaccgagg gcggcggcgg ccggcccggc
     1561 gacacggacc aggcgatggt cgcgggcctg gacacggtcg ccttcgcgct gtgggccaag
     1621 ctgtccggcg tggtgccgcg catcggcgtc gcgtcagggc gctgcttcgc gggcaacgcg
     1681 gccctctacg ggatgtgcga cgtgaccatc gccacgcgcg aggcgtcgat cggcatgggc
     1741 gggccggcca tgatcgaggg cggcggcctg ggcgtggtgg acccggacga cgtgggcccg
     1801 ctgtcggtgc aggtgcccaa cggcgtcgtg gacatcgcgg tggacgacga cgccgccgcg
     1861 gtggccatgg ccaagcgcgt gctgtcctac ttccagggtg ccgtgacggc atgggaggcc
     1921 ggcgaccagc ggctgctgcg cacgctggtg cccgaggacc gcaagcgcgc ctaccgcgtg
     1981 cggccggtca tcgacgcgct gtgcgacgcg gggtcggtca cggagctgcg cgaggcgttc
     2041 gggcccggca tggtgaccgc gctggcgcgc ctggagggcc ggcccgtcgg catcgtggcc
     2101 aacgacccgg tgcacatggc cggcgccatc accagcgacg gggcggacaa ggccgcgcgg
     2161 ttcctgcagc tgtgcgaggc gttcgggctg ccggtgctgt tcctgtgcga cacgccgggg
     2221 atcatggtgg gcccggaagc ggaggcgacg ggcctggtgc gtcatgccgc gcggctgttc
     2281 ctggcgggtg gcgccttgac ggtgccgttc gtcacggtgg tgctgcgcaa ggggtacggc
     2341 ctcggcgcac aggcgatggc gggcggcagc ttccgctcgc cgctggcgac gctgtcctgg
     2401 ccgaccggcg agttcggcgg gatggggctg gagggggcgg tgaagctggc gctgcgcaag
     2461 gagctggccg cgatcgagga tccggcggag cgccaagcgg cgttcgacaa cgcggtggcg
     2521 tgggcgtacg agcagggcaa ggcgacgtcc atggcgacct acttcgagat cgacgacgtg
     2581 atcgacccgg ccgacacgcg gcacgtcgtg ctgcagttgc tgcgctcggc gcccccgcac
     2641 gcctcccccg acgcatcgca cgcacggcgc ccgagatacg tcgacgcctt ctgaccattc
     2701 tcggcgcggc tagcggcgca ccgccgacac gcgcccggtg tcgtcgaagg ccacgaccac
     2761 cttgcccgcg cagtagctga acgagcgcgc gccgcgctgc tccggctggc ccagcgccag
     2821 cagcacctgc tccggcgtca tgccgcgccg cacgcgggag tccgcccggc cggcggtctg
     2881 cgcggccgca cagccgtccg gtgcgatgcc cagcgcccgc tcccacatct gcaggtacgc
     2941 ctcggggccg cgcgcgaggt cttcgacgat ctcgtcgccg gccagcttgc gcaggtcctc
     3001 gatccagtcc gggtacaggc cgtagtgcgc gacgccgtcg gtgttgatgt cgtagacgcg
     3061 ctcaccgctg cgctgccggt gcaccgtgac tcccccgaag ccggtgaacg ggtaggtcac
     3121 cttgttctcc gcaccggcgc cgcgcggcgc gccctggctg ccgaagccgt tggtgtccgc
     3181 gccgtagccg aagccccagt accagcgcgg gtcggcccac tgcttgtgct ggcgccagtt
     3241 cgccacgaac gacgtggagc tgccgccgta gggcgtgacc acgccgccct gccggtagat
     3301 gcgcgggtac gtggtctcgt cggcccagcc gtggctggac accacgcccg ggtagccgag
     3361 ctcctcgacg atgtccatcg cctgcgtgcg ggcgcgcgcg ctcatgtggt cggggtcgaa
     3421 gatcatgccc ttctgcacca tcttgcggat gaggtactcg cccaggtgcg tcaggccgcg
     3481 cacgttgcag tgcggcccct cggggtacac cggcgcggcg ccggacgtgc cggtcagccg
     3541 caggatgccg ccggccagcg cgtcgcgtcc cgtcagctcg tccggcgaac cgctgtcgtc
     3601 gtggaagttg tgctgctggc ggtcgtgcgc gtgtccgtcg gcttcggtgc acgtctccat
     3661 ctgccagaac ttgccggtct cgatgaagtt gccgttgttc accacgatgc cggtggaccc
     3721 ggcgtcgccg gcgacgcccg ccagcgcgtt gtcgaacttg ttgacgaact ccatctggcg
     3781 cacgcccagc gcgtgcacct cgtcgagctg cttgtcgatc tgcgcgtggt cgcagcgggc
     3841 ctgctcggcg aacacgccgc agtcgaacag cacactcacc tcgataccca gcaccacggc
     3901 gagcttgccg gcgttcatca cgcgccgcgc ctcgaacggg tcggtgacga tgcggaacca
     3961 gccctcaccg ggtccgccgc tctgcgcgtc gatgtagcgc tccagctccc ggatgcgctg
     4021 ggcctgcagg cgcacgccgt ccatctcgtt gcagccgtgc gacttgtacg ggtagatctc
     4081 gcacagcacg ttgttgtcga ccagcaggtt gacgaacatg cgcaggccgc cgcgccagga
     4141 gcgctccagc cacttgtagt agacctgctc gtgcgtcagc gagtcgtaac gcggccagta
     4201 gccgaacgtc ggccagccga cggtgtcgtg cacgggcacg tcgccaccgg ccagcacgtt
     4261 ctccagcacg gcaccggccc cgcccggctc gtggtccggg cagtcgacca gcgcgtaggt
     4321 gaccccgtag cggtgccagg gccggccgca gcgcgcgcgg ccgcccagga actcgaacgc
     4381 catcatgtgc aggtgggcgt ccaggaaccc gcgcacctcg ccggtgggcg acgcgccgat
     4441 ggccaccggg ccgtccacgc cgatctccac ctcggggaag gtcgcgcagc cctgcgtccg
     4501 ggtgaacgcg aagcgcgtcg ccttgccctt gtcggcggcg tcgaccagga ccagccggcc
     4561 gtcgccgccg gccgcgagcg ccttgccggc cgcgggcagg gtgatgcgga agccgttgcc
     4621 gtgctcgtcg acgtcccagt cggccagctc gctgggcgcc gccgcgctga ccacggccgc
     4681 gccccggccg gtcgcgtccg cggccgcgcc gtcgcccacg gcgcgcgcgg ccacgccggt
     4741 ggcgccgatc gaggctccct cggcgatgcc gcccggcccg ctgccggtgg cggcgtcggc
     4801 cgcggtgccc agcggcgagt cggacgcggc gacgaagtcc tgcgccgacc cgtacagcag
     4861 gtagcggccg aggtcggtcg cctggaagtg gaagggctcc gccccggcgg cgtccgccgt
     4921 ggccgcgaac ccgccgccgt cgcggcgcac gaagcccccg ctggcggccg actgcacggc
     4981 ccagcacccg ccggccaggt cgtagcggtc ccccggctcg cgcccctgcg ggggatccgc
     5041 tggcggctgc tgcggtccgg tcgtgtccgc cagcgcctgg atgatctggg cggcgggggt
     5101 ggccgggtcc ggctccacga cgggaaccac ggcgccggag ggcgccgccg cgagcagcgc
     5161 gacgcccagc atggcggcaa gggggatcat ccagacgggg cggcgctggc gcatggcgaa
     5221 cctcatggga cggcgggaca tggcgttcag ggggactatt cgccgccgcg ccgcccgatc
     5281 ccttccgccg gcgccgcgcg gccagggcgt cagggctgcg cccaggtcca ctcgtcggcg
     5341 agcagcgcgc gggcgtccag cgacgcgccg tcctggacgt cggcgcgcga gcgcggtggc
     5401 agggggctgt gccgcgacag caccgcgcgg atcgtggaac cctcgctcgt gctgagcacg
     5461 tcgacgcgct gggccaccgc gcggacgatg tacaggccgc gaccggcttc ggtgtccgcg
     5521 ggcggcaggg cgcgcccgcg ctggtccagg tccagggccc cctggccgtc gtcggagacg
     5581 tccaccacga tggccgcgtc ggtgaggcag acgccgagca ccacgcgccc gcgcgcgctg
     5641 cgcatggcgt tggacaaaag ctcgccggcc gccaggctgg cgtcatgggc gtcgaccccg
     5701 cggcgcgaca gccagtcgcg cagcgcgcgg cgtgtctggc tgatctcggc gggatccggg
     5761 gaggcctccc agctggcgcg ctggtcctcc gcgtgcaccg ggtcgcggcg cagcaccagg
     5821 gccagcgtgt cgtcgcggcg ctcggcgccg gccagggcgc gcgacaccag ctcgcgcgcg
     5881 aactcgtccg ccggcaggtc ggccacctcg gcggcgtgcc ggaccaggtc gtccatgcca
     5941 tccaggatgt tcttgcgcgc ctcgaccagg ccgtcggtgt acatcaccag cgcgtcgccc
     6001 ggctccagca cggtctcgga gatcgtgtcg ctgccggcgc cgggccagcc gatcacgccg
     6061 ccggaggcgg cgacctgcgt gacctcgcgg cgcggggtca ccacgagcgc cggcgggtgg
     6121 ccgccggcgg cgacccgcag ccgcccggtg tcgggcgcgt agcgcgcgac gatgacggtg
     6181 gccacgagct cggggtgctg ggcctgcagc aactcgtcgg cgcgggacac caaaagctcc
     6241 agcggcgtgc cggacgcggt caggacgcgc agcgtgtgca ccacggcgag cgcgtccttg
     6301 gtggcggcca cgccgtggcc cagcacgtcg accacggcca cgtgcacctc cccggagggc
     6361 agcacctgcc agtcgtacag gtcgccgccg gtgggggcct tggggtcgga ggactcgtag
     6421 gcggcggcca gctcggctcc cttcacggcg atgggggccg gcaccacggt ctgctggagc
     6481 tgctccacca ccacgcgccg cgccgcgtcg gcttccagca gctgggagaa ctgcgcgcgc
     6541 agcgcctcgt gctcctccgc cggcgtcacg tcgcgggcca ccacgatcag cgccgccccg
     6601 ccctgctcca tggagccggc gctgtaggag cacgtcaggt gccggcggcg gccgtccatc
     6661 gccatcaggc ggatgtcgcg gggcagggat tccagcgagc cgcggcgcag gtcgacctgc
     6721 tcgccggcgg cggtgcggac gcgcaggcgc cggccgatgt ccctggtgcc caccacggac
     6781 tcggccggca agccggtgat gtgctccagc gccgggttcc acgtcaggac cgtgccgtcc
     6841 tgggagaacg tgcagatgcc gtcggaggcg ctgtcgacga tctcggcgag cttgcgccgc
     6901 tcctccagca cgtcgcccag cagccgcccc ttggccagcg cgccggcggt ctcacgcgcc
     6961 agcgcctcga agatggccag ctcgccgctg cgggagcccg cgaagccgcc gcggtcgagc
     7021 accagcacgg cgcccataag gcggtcgccg tccatgagcg gcgccgccag gcagtccttg
     7081 gcgccggcgg cgcgcagcgc gcgcgacacc gcgtcgccgt cgtcggccgt gaccgcgacg
     7141 gcgcccggcg aggccgccag gaccccctcg agcgaggcga cgttctcgtc ctccaggcgc
     7201 gagccgcagc cgccccggtc acggtcgtgg cggtggatct cgcggccgcc gtccacgcgc
     7261 agcaccagct cggcggcgtg cgcgtcgaag gccacggtca gctccgccag gtactccggg
     7321 atggcggcca tgggctgcag cggttcggcc aggcgccgcg c
//
LOCUS       DATMKC010000222         4106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_197422, whole genome shotgun sequence.
ACCESSION   DATMKC010000222 DATMKC010000000
VERSION     DATMKC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197422"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VNU01_06480"
     CDS             complement(<1..198)
                     /locus_tag="VNU01_06480"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HWH32301.1"
                     /translation="MTPRTPEAPDRNLALELVRATEAAALAAGRWMGRNQKESGDQAA
                     VDAMRLLLATVSMDGVVVIGEG"
     gene            complement(283..1041)
                     /locus_tag="VNU01_06485"
     CDS             complement(283..1041)
                     /locus_tag="VNU01_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWH32302.1"
                     /translation="MTVIVEPHAELRVLRMSRPEARNAMNTAMLMALLDGLADAVADD
                     GVRGVVLTGDAGSFSAGADLREELDRDGQVRRMDLFCQLYEAVGTCPKPTVAAIEGHC
                     VGGGIEVAAACDLRVADTTARFRFPGAALGIPVGAAKLVGLVGLGTAKDLVLTSRTFD
                     AEEALRIGFAQRLAEAGSALEGALEVAGQIAANNLDAVRYLKRQFLAFSGSGDRIAAE
                     NDALHAVTESGGDYAALTMPDPKAAWLRGAGKVR"
     gene            1224..3641
                     /locus_tag="VNU01_06490"
     CDS             1224..3641
                     /locus_tag="VNU01_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWH32303.1"
                     /translation="MSRELEEPDGLRRTDAAMRRVRWAGCAFVLLQFGLYQAPAGVEM
                     PFARWPVAFLLAGVLAALNVAAMATRHVFGERGLRALGRAQWALDAAVIMAVVWLFSF
                     DSTSALWALLVVPILEGALRGQLAGALGTWASLGVLYLVRDAWAAATYSHREFLVPSV
                     TYRLGILLLVALTAGMLARNLRDQMRAHAGLRLVAERRAELLGVVASAGRTPATDVES
                     VLAGLADAALRLGFDACEICVLDHDGETYTVRCAKGLPAAYERGAVPATTGLAGVAIA
                     RRETVVVDDYSSWPGGVRTVREAGFHTAVAAPIISAGEVVGVMAAGRLDHEPTDEDQV
                     DCLELLATNAGAAVENQRRLAERASYEDRLTHQAYHDSLTGLPNRARFMEVLDASIRD
                     RLEEVAVCYVDLDRFKTVNDSLGHEVGDELLRAVADRLREHTPADGLVARLGGDEFTV
                     LLRGPGALTRAPRLAQRLTAALRAPYQLGERELYVSGSVGVSMSGGAEHGSSELLRRA
                     DLAMYRAKEQGRDRVALYQPALGERLIRRMEVETGMRRALEKGEFRLHYQPIYSLAEG
                     TMVGAEALLRWQRDGVTVPPAEFLTAAEETGVIVPLGGWVLREAASQHRAWLDRHGVA
                     LPLSVNVSAVQLRHPKLADHVERALELSGMEPGMLVLEVTEGGIAADVEGLAQLHRLR
                     ALGVRLALDDFGTGWSSLTRLRGMPFDILKIDKGFVARLPDDERSIAIVRSVVSLAEE
                     LGMTVTAEGIEHDEQRWAVQALGCTHGQGYHLGHPMEADIAEALVASLSPLRKVRLLG
                     RAVETGR"
     gene            3673..>4106
                     /locus_tag="VNU01_06495"
     CDS             3673..>4106
                     /locus_tag="VNU01_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32304.1"
                     /translation="MGIKDALLPPDQPRWLTAAFAPALVGHGVAGFGLYWDIAWHIDA
                     GRDEKLLTAPHTLILAGLLLIAVSACAGLYLAAREGRRLPRGLLAAAFAGGMAVIGFP
                     LDELWHSARGRRRPSRAARYSPAHADTAMSSRPARISVCGAV"
BASE COUNT          506 a         1506 c         1530 g          564 t
ORIGIN      
        1 gccctcgccg atcaccacca cgccgtccat cgacacggtc gccagcagca gccgcatggc
       61 gtccaccgcc gcctggtcgc cggactcctt ctggttgcgg cccatccagc ggcccgcggc
      121 cagcgccgcc gcctcggtgg cgcgcaccag ctccagcgcc aggttgcggt cgggggcctc
      181 gggcgttcgt ggcgtcactg cgtcacctca tgacagcgat tcgggcgggc tcgggggctt
      241 ggccgggagc atacccagcc cgcaaacatc gccgctcggg tgtcagcgca ccttgccggc
      301 cccgcgcagc caggccgcct tggggtccgg catcgtgagc gccgcgtagt cgccgccgga
      361 ctccgtgacg gcgtgcagcg cgtcgttctc cgcggcgatg cggtcccccg agccggagaa
      421 cgcgaggaac tgccgcttga ggtagcgcac cgcgtcgagg ttgttcgcgg cgatctggcc
      481 cgccacctcc agggcgccct ccagggcgga ccctgcctcg gccagccgct gcgcgaagcc
      541 gatgcgcagg gcctcctcgg cgtcgaacgt gcgcgacgtc agcaccaggt ccttcgcggt
      601 gcccaggccc accaggccga cgagcttggc ggcgccgacc gggatgccca gggcggcgcc
      661 cggaaagcgg aagcgcgccg tggtgtccgc cacgcgcagg tcgcaggcgg ccgcgacctc
      721 gatgccgcca ccgacgcagt ggccctcgat cgcggcgacg gtcggcttgg gacaggtccc
      781 gaccgcctcg tagagctggc agaacaggtc catgcggcgg acctgaccgt cacggtccag
      841 ctcctcgcgc aggtccgcgc cggcggagaa ggagcccgcg tcgccggtca gcacgacgcc
      901 gcgcacgcca tcgtcggcga cggcgtcggc caggccgtcc agcagcgcca tgagcatcgc
      961 cgtgttcatg gcgttgcgcg cctcggggcg gctcatgcgc agcacgcgca actcggcgtg
     1021 cggctcgacg atcacggtca cgggctgctc ctgtcacgtc ggagccgccc agcctagtcg
     1081 gtacggactg caggcaaatg acccatccgg gccatggcgg cgcgcgcggg ccgctcccat
     1141 gctggtgtgt ggcccacccg tcacggtggc gccgggcggg cggcgcacgc gcgcacacgt
     1201 tgcgagggga acgaggtcgg taggtgagcc gcgagcttga ggagccggat ggcctgcggc
     1261 ggaccgatgc cgcgatgcgc cgggtccggt gggcgggctg cgcgttcgtg ctgctgcagt
     1321 tcggcctgta ccaggcgccc gcgggcgtgg agatgccgtt cgcgcgctgg cccgtggcct
     1381 tcctcctggc cggcgtgctc gccgcgctga acgtggccgc catggccacg cgccacgtct
     1441 tcggcgagcg cggcctgcgc gcgctcggcc gtgcccagtg ggccctggac gccgccgtga
     1501 tcatggccgt ggtatggctg ttctccttcg actcgacttc ggcgctttgg gcgctgctgg
     1561 tggtgccgat cctggagggg gcgctgcggg ggcagctggc cggcgcgctc ggcacctggg
     1621 cctcgctcgg cgtgctgtac ctggttcgcg acgcgtgggc ggcggccacc tacagccacc
     1681 gggagttcct ggtgccgtcg gtgacctacc ggctgggcat cctgctcctg gtggcgctga
     1741 cggccgggat gctggcgcgc aacctccgag accagatgcg cgcgcacgcc gggctccgcc
     1801 tggtcgcgga gcggcgggcc gagctgctcg gcgtcgtggc ttccgccggg cgcacccccg
     1861 ccaccgatgt cgagtcggtg ctcgccggcc tggccgacgc cgccctgcgg ctgggcttcg
     1921 acgcgtgcga gatctgcgtg ctggatcacg atggcgagac gtacaccgtg cgctgtgcca
     1981 agggcctgcc cgccgcctac gagcgcggcg ccgtcccggc caccaccggc ctcgccggcg
     2041 tcgcgatcgc gcggcgggag accgtcgtgg tcgacgacta cagcagctgg cccggcggcg
     2101 tgcgcaccgt gcgggaagcc gggttccaca ccgccgtggc ggcgcccatc atctcggccg
     2161 gtgaggtcgt cggggtcatg gccgccgggc gcctggacca cgagccgacc gacgaggacc
     2221 aggtcgactg cctcgagctg ctggccacga acgccggtgc cgccgtggag aaccagcgcc
     2281 gcctcgccga gcgcgcctcg tacgaggacc gcctcaccca tcaggcctat cacgactccc
     2341 tgaccggcct gcccaaccgc gcgcgcttca tggaggtgct ggacgcctcc atccgcgacc
     2401 ggctggagga ggtcgcggtg tgctacgtcg acctggaccg gttcaagacc gtcaacgaca
     2461 gcctgggcca cgaggtcggc gacgagctgc tgcgtgccgt ggccgaccgg ctgcgggagc
     2521 acacgcccgc ggacggcctg gtggcccggc tgggcggcga cgagttcacc gtgctgctgc
     2581 ggggtcccgg cgcactgacc cgcgccccgc gcctggccca gcggctcacc gccgccctgc
     2641 gcgccccgta ccagctgggc gagcgggagc tgtacgtctc cggcagcgtc ggcgtgtcca
     2701 tgagcggcgg tgccgagcac ggctccagcg agctgctgcg gcgcgccgac ctggccatgt
     2761 accgggccaa ggaacagggc cgcgaccgcg tcgccctgta ccagccggcg ctgggtgagc
     2821 ggctcatccg ccgcatggag gtcgagaccg gcatgcggcg agcactggag aagggcgagt
     2881 tccgcctgca ctaccagccg atctactccc tggccgaggg caccatggtg ggcgcggaag
     2941 cgctgctgcg ctggcagcgc gacggcgtca cggtgccgcc ggcggaattc ctcaccgccg
     3001 cggaggagac cggcgtgatc gtgccgctcg ggggctgggt cctgcgcgag gcggccagcc
     3061 agcaccgagc ctggctggac cgccacggcg tcgccctgcc cctgtcggtg aacgtgtctg
     3121 ccgtacaact gcgccacccc aagctggccg accacgtcga gcgcgcgctg gagctgtccg
     3181 gcatggagcc ggggatgctc gtgctggagg tgaccgaggg cggcatcgcc gccgacgtcg
     3241 aggggctggc gcagctgcac cgcctgcgcg cgctgggcgt gcgcctggcc ctggacgact
     3301 tcggcaccgg atggtcctcg ctcacccgcc tgcgcggcat gccgttcgac atcctcaaga
     3361 tcgacaaggg gttcgtggcg cgcctgccgg acgacgagcg cagcatcgcg atcgtgcgca
     3421 gcgtggtcag cctggcagag gagctgggca tgacggtgac cgcggaaggc atcgagcacg
     3481 acgagcagcg ctgggccgtg caggcgctgg gctgcaccca cgggcagggc taccacctgg
     3541 gccacccgat ggaggcggac atcgccgagg cactggtggc gtcgctgtcg ccactgcgca
     3601 aggtgcgcct gctgggacgc gcggtggaga cgggtcgttg atccgactgc tccactgcgc
     3661 gagcatgcgc gcgtggggat caaagacgct ctgctgccgc ccgatcagcc gcgctggctg
     3721 acggccgcct tcgcgcccgc cctcgtcggc cacggcgtgg cgggcttcgg cctgtactgg
     3781 gacatcgcct ggcacatcga cgcgggccgt gacgagaagc tcctcaccgc tccgcacacg
     3841 ctgatcctgg ccggcctgct gctcatcgcc gtgtccgcgt gcgcggggct gtacctggcg
     3901 gcgcgcgagg gacggcgcct gccgcgcggc ctgcttgccg ccgccttcgc gggcggcatg
     3961 gccgtgatcg gcttcccgct cgacgagctg tggcactccg cgcgcggcag gcgccgtccc
     4021 tcgcgcgccg ccaggtacag ccccgcgcac gcggacacgg cgatgagcag caggccggcc
     4081 aggatcagcg tgtgcggagc ggtgag
//
LOCUS       DATMKC010000223         4184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_296946, whole genome shotgun sequence.
ACCESSION   DATMKC010000223 DATMKC010000000
VERSION     DATMKC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296946"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..2086
                     /locus_tag="VNU01_06500"
     CDS             20..2086
                     /locus_tag="VNU01_06500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWH32305.1"
                     /translation="MPVHPLDALDAILSAHPGASRRPGQEAMCAAVAQAVAERRHLLV
                     EAGTGVGKSFAYLAVAATLGRRVVVATATKALQEQLVGKDLPAVADALEPLLGRRLRF
                     AMLKGRGNYLCLAKAASASRAMAGLDDTLLADLPSRADDEDAVAQLAWWAWQTETGDQ
                     AEVPFELKPAQWRHVSTDARECPGAGDCAFGEECFAERARRQAMSADIVVVNTHLYTL
                     HAMLEAEGAGLLPPHDLVIVDEAHTLEDIAVGAFSVEVSGVRLRWLAGRVRAIVDGGK
                     ADAALQRAADGLDVALGPLRGERVDPADGSLGAALAATSKAALAAVEELSGIAPTSED
                     AVTRHRMAMRAAESLVTDLAVARAAGAGEVAWVKDSGALAVSPVEVGPALADTLFSRR
                     TAVLTSATLGIGGSFEPRAFRLGLGEERPLTPADDGRGAWPPEAAVDEDGEPEPHTLE
                     WSGLAVESPFDYQASTLLYCAVDLPEPNSDAFRDAATRRLAELMRAAGGRTLALFTSH
                     AALRHAAEALPGMVDFPLLVQDEAPRGLLMERFVAEEEACLLATLGFWQGVDVPGRSL
                     SLVVVDRLPFPHRNDPLMQARRELATARRRSAFMEVDLPHAATLLAQGVGRLIRSSAD
                     RGAVAVLDRRLATKDSYRPTLLRSLPPMPRTRELSEAVAFLEHCVTAAAGMPAGGARG
                     AAGARG"
     gene            2115..3590
                     /locus_tag="VNU01_06505"
     CDS             2115..3590
                     /locus_tag="VNU01_06505"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWH32306.1"
                     /translation="MPQLHAAAADPRQVDADLVVVPVYKAGIEGPGAGLVLRALGMDA
                     FPVTPSFRGDIGQHLLIAAPGLAAGGVLLVGLGRMDATSPAQLRRAAGVAARAATGAR
                     RVATTLAEVHPSAEAVQAVAEGFLLGAYRDRRFRSSGDGDQPELESLTVLVPSSHVAA
                     AEQALGRADIAVRATITARDLVNLPPDRKRPPAFADTVAELLAGRCEVRVRDEHDLEA
                     EGFGGILAVGRGSEAPPRLVELRYRPASPIGHVVLVGKGITFDSGGLSLKRGGGMNTM
                     KSDMAGAAAVVGVFDALAELDVPLEVTGLLACAENMPGGDAQRPGDVLRAYGGITVEV
                     LDTDAEGRLVLADALAYAAGMKPDAIVDLATLTGSIVTALGRYAAGVMGTDDGLRDAL
                     CRAAEGAGEPLWPMPLWPELERFLDSAVADINNTADGPGGGAVTAAMFLRRFVGEVPW
                     AHLDIAGPAFLPGDLADGYLPPGGTGYGVRTLLAWLEHRAA"
     gene            complement(3602..>4184)
                     /locus_tag="VNU01_06510"
     CDS             complement(3602..>4184)
                     /locus_tag="VNU01_06510"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HWH32307.1"
                     /translation="YGTFTTHRPVAPPPGPTARTRWPPRDRRWCPRRRSASARWGPAE
                     AEALFARLGPDPLRRDGRPGPFLDALRARRTPVGAALLDQSLIAGVGNVFRAEALFAC
                     GIHPERPASALSEDEARCLWDTVTRMLRDGVRRNRIATVPAAERDRREPEGRGKDRFV
                     YHRAGLPCRRCGTAVVEKPLAGRRLFFCPSCQT"
BASE COUNT          478 a         1601 c         1598 g          507 t
ORIGIN      
        1 ccgatggcac catgacgtga tgcccgttca ccccctcgac gcgctggacg cgatcctgtc
       61 cgcccacccc ggcgcctcgc ggcgccccgg gcaggaggcg atgtgcgccg cggtcgcgca
      121 ggccgtggca gagcggcgcc acctcctggt ggaggccggc accggcgtgg gcaagagctt
      181 cgcctacctg gcggtcgccg ccaccctggg ccggcgcgtc gtcgtcgcca cggccaccaa
      241 ggcgctgcag gagcagctcg tgggcaagga cctgcccgcg gtcgccgacg ccctggagcc
      301 cctgctgggc cgccgcctgc gcttcgccat gctcaagggc cgcggcaact acctgtgcct
      361 ggccaaggcc gcctcggcct cgcgcgcgat ggccgggctg gacgacaccc tgctcgcgga
      421 cctgccgagc agggccgacg acgaggacgc cgtcgcccag ctcgcctggt gggcgtggca
      481 gaccgagacg ggtgaccagg ccgaggtgcc gttcgagctc aagcccgccc agtggcggca
      541 cgtcagcacc gacgcacgcg agtgcccggg tgccggcgac tgcgccttcg gcgaggagtg
      601 cttcgccgag cgggcgcggc gccaggccat gagcgccgac atcgtggtgg tgaacaccca
      661 cctgtacacg ctgcacgcca tgctcgaggc ggagggcgcg gggctgttgc cgccgcacga
      721 cctcgtcatc gtggacgagg cgcacacgct ggaggacatc gccgtgggcg cgttctccgt
      781 ggaggtctcc ggcgtgcggc tgcgctggct ggccgggcgc gtgcgcgcca tcgtcgacgg
      841 cggcaaggcc gacgccgcgc tgcagcgcgc cgccgacggc ctcgacgtgg ctctcgggcc
      901 gctgcgcggc gagcgcgtcg acccggccga cggcagcctc ggcgcggcgc tggccgccac
      961 gtccaaggcc gcgctggccg ccgtggagga gctgagcggc atcgcgccca cctccgagga
     1021 cgcggtaacg cgccaccgca tggcgatgcg agccgccgag tcgctggtca cggacctggc
     1081 cgtcgcgcgg gcggccggcg ccggcgaggt cgcctgggtc aaggacagcg gcgcgctggc
     1141 ggtctcgccg gtcgaagtcg gcccggccct ggccgacacg ctgttctcgc ggcgcaccgc
     1201 ggtgctcacg tcggcgaccc tgggcatcgg cggctccttc gagccgcgcg cgttccggct
     1261 cggcctcggc gaggagcgcc cgctcacccc cgcggacgac ggccgcggcg cgtggccgcc
     1321 cgaggccgcc gtggacgagg acggcgagcc ggaaccgcac acgctggagt ggtcgggcct
     1381 ggcggtggag agcccgttcg actaccaggc cagcacgctg ctgtactgcg ccgtggacct
     1441 gcccgaaccc aacagcgacg cgttccgcga cgcggccacg cggcgtctgg cggagctgat
     1501 gcgcgccgcg ggcggccgga cccttgcgct gttcaccagc cacgcggcgc tgcgccacgc
     1561 cgccgaggcg ctgcccggca tggtcgactt cccgctgctg gtccaggacg aggcgccgcg
     1621 cggcctgctg atggagcgct tcgtcgccga ggaggaggcg tgcctgctcg ccacgctggg
     1681 cttctggcag ggcgtcgacg tgccgggccg cagcctcagc ctggtggtcg tggaccggtt
     1741 gccgttcccg caccgcaacg acccgctcat gcaggcccgc cgcgagctgg ccaccgccag
     1801 gcgccgctcg gcgttcatgg aggtggacct gccgcacgcc gcgacgctgc tcgcgcaggg
     1861 cgtcgggcgg ctgatccgct ccagcgccga ccggggcgcg gtcgcggtcc tggaccgccg
     1921 cctggccacc aaggacagct accggccgac gctgctgcgc agcctcccgc ccatgccgcg
     1981 cacgcgcgag ctgagcgagg cggtggcgtt cctggagcac tgcgtcacgg ccgcggcggg
     2041 catgcctgcc ggcggggccc gcggcgccgc cggtgcccgg ggctagaatc ccgctcccgc
     2101 caggaggcca ccacatgccg cagttgcacg ccgccgccgc agacccccgc caggtcgacg
     2161 ccgacctcgt cgtcgtgccc gtctacaagg cgggcatcga gggacccggg gccggcctgg
     2221 tgctgcgcgc gctgggcatg gacgcgttcc ccgtcacgcc gtccttccgg ggcgacatcg
     2281 gccagcacct gctgatcgcc gcgccgggcc tcgccgcagg cggcgtgctg ctggtcggcc
     2341 tggggcgcat ggacgccacg tcgcccgcgc agcttcgccg ggccgccggc gtggccgccc
     2401 gcgcggcgac cggagcgcgc cgcgtcgcca ccaccctggc ggaggtgcat ccctccgccg
     2461 aggcggtcca ggccgtggcg gagggcttcc tgctgggcgc ctaccgcgac cggcgcttcc
     2521 gctcctccgg cgacggcgac cagcccgagc tggagtcgct gaccgtgctg gtgccgtcga
     2581 gccacgtcgc cgcggcggag caggcgctgg ggcgcgccga catcgcggtg cgcgccacca
     2641 tcaccgcccg tgacctggtg aacctgcccc ccgaccgcaa gcgcccgccc gcgttcgccg
     2701 acacggtcgc ggagctgctg gcgggccgct gcgaggtgcg cgtgcgcgac gagcacgacc
     2761 tggaggccga gggcttcggc ggcatcctgg cggtggggcg cggctcggag gcgccgccgc
     2821 ggctggtgga gctgcgctac cggcccgcga gcccgatcgg tcacgtggtg ctggtgggca
     2881 agggcatcac gttcgacagc ggcgggctgt cgctcaagcg cggcggcggc atgaacacca
     2941 tgaagtccga catggccggc gccgcggcgg tcgtcggcgt attcgacgcg cttgccgagc
     3001 tggacgtgcc gctggaggtc accggcctgc tggcgtgcgc ggagaacatg ccgggtggcg
     3061 acgcgcagcg ccccggcgac gtgctgcgcg cctacggcgg catcaccgtg gaggtcctgg
     3121 acaccgacgc ggaggggcgc ctggtcctgg ccgacgcgct cgcgtacgcc gccgggatga
     3181 agcccgacgc catcgtggac ctggccaccc tcaccggctc catcgtcacc gccctgggcc
     3241 gctacgccgc cggtgtcatg ggcaccgacg acgggttgcg cgacgcgctg tgccgcgccg
     3301 cggagggcgc cggcgagccg ctgtggccga tgccgctgtg gccggagctg gagcgcttcc
     3361 tggactccgc cgtcgccgac atcaacaaca ccgccgacgg gccgggtggc ggcgcggtca
     3421 ccgccgcgat gttcctgcgc cgcttcgtgg gcgaggtgcc gtgggcgcat ctggacatcg
     3481 ccgggccggc gttcctgccc ggggatctgg ccgacgggta cctcccccca ggcggcaccg
     3541 gctacggcgt gcggacgctg ctggcctggc tggagcaccg cgccgcctga tccaccccgg
     3601 gtcaggtctg gcaggagggg cagaagaaca gccgccgccc ggcgaggggc ttttccacca
     3661 ccgccgtccc gcagcggcgg cacggcagcc cggcgcggtg atacacgaag cggtccttgc
     3721 cgcgcccctc cggctcgcgc cggtcgcgct cggccgccgg caccgtggcg atgcggttgc
     3781 gccgcacgcc gtcgcgcagc atgcgcgtca cggtgtccca caggcagcgc gcctcgtcct
     3841 cgctgagcgc gctcgccggg cgctccgggt ggatgccgca ggcgaacagc gcctcggcgc
     3901 ggaagacgtt gccgacgccg gcgatcagcg actggtccag cagcgcggcg ccgacgggcg
     3961 tgcgccgcgc gcgcagcgcg tccaggaacg gtcccggcct gccgtcgcgg cgcaaggggt
     4021 ccggtcccag gcgcgcgaac agcgcctccg cctccgccgg gccccaacgt gcgctggcgc
     4081 ttcggcgccg aggacaccac cgtcgatctc gtgggggcca ccgcgtgcga gctgtggggc
     4141 ccggcggcgg cgccacgggc cggtgcgtgg tgaacgtgcc gtac
//
LOCUS       DATMKC010000224         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_80746, whole genome shotgun sequence.
ACCESSION   DATMKC010000224 DATMKC010000000
VERSION     DATMKC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80746"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VNU01_06515"
     CDS             <1..264
                     /locus_tag="VNU01_06515"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32308.1"
                     /translation="HLGGSARAVKLPGDYRRAAVAAARHMGLNVAGVDLLPSDEGPMV
                     LEVNSSPGLEGIEKSTKADVADAVIAYIERNVRKGSSYDRGGR"
     gene            261..1304
                     /locus_tag="VNU01_06520"
     CDS             261..1304
                     /locus_tag="VNU01_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HWH32309.1"
                     /translation="MIGAGTLLLGGAEVPQGARTDLLLKVSESYTGDRTSIPVTVVNG
                     DQPGPVLFVTAAIHGDELNGIGIVRDLLGRVEPGAVAGALICVPVVNVLGMQFHSRYL
                     PDRRDLNRCFPGSAGGSTASRIAHTVMTEIVQQSDAGVDLHTAANRRMNLPQVRTSLA
                     DPRARDLARAFGAPFVIDASIRPGSLRDAADRVGVPVLTYEGGQALRFEDDVIAVGVA
                     GVLRVMGALGMLDHAPPQGDGDVVECDETHWIRADHGGILDLHVAPGDLVMEGQPLWT
                     VTGPFGRERSQKVSPYEGYVIGMTTLPLVNPGDAVLHIAVPGVRGAASIDEPTDEEDL
                     LGVESGEGDEETD"
     gene            1306..>2048
                     /locus_tag="VNU01_06525"
     CDS             1306..>2048
                     /locus_tag="VNU01_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWH32310.1"
                     /translation="MAGRAEPWLTPQRLLGLVLTLVGAWVALQVVRALRDVLIMLLVA
                     LFLSFAMEPAVQHLARRGWRRGAATGAVFAVFALVGMLVLAAMTPLVITQVNGLIESA
                     PRSVNEVIEAANRLPFVNLEATPELRQEIEDFGERFGERLSSAALSAAGDVVSVGGAA
                     IGLVIQVLAIGLVTFYLVADGPRFRRTLARPLQPARQREMLAIWEMAVAKTGGYIYSR
                     VLLAVIAAVVHAVALLVLGVPFPLPLGIW"
BASE COUNT          264 a          722 c          774 g          288 t
ORIGIN      
        1 cacctgggcg gctcggcgcg cgcggtcaag ctgccggggg actaccggcg cgccgccgtg
       61 gccgccgccc ggcacatggg gctgaacgtg gcgggcgtgg acctgctgcc cagcgacgag
      121 ggccccatgg tcctggaggt caactcctcc cccgggctgg aggggatcga gaagtccacg
      181 aaggccgatg tcgccgacgc cgtgatcgcc tacatcgaac gcaacgtgcg caagggctcc
      241 agctacgacc ggggcggccg gtgatcggcg ccggcacgct gttgctgggc ggcgccgagg
      301 tcccgcaggg cgcgcgcacg gacctgctgc tcaaggtcag cgagagctac accggcgacc
      361 gcacctccat cccggtcacc gtggtcaacg gcgaccagcc cggcccggtg ctgttcgtga
      421 ccgccgcgat ccacggcgac gagctcaacg gcatcggcat cgtgcgggac ctgctggggc
      481 gggtggagcc gggcgccgtc gccggcgcgc tgatctgcgt gcccgtcgtc aacgtgctcg
      541 gcatgcagtt ccacagccgc tacctgccgg accgccgcga cctcaaccgc tgctttcccg
      601 ggtccgccgg cgggtcgacg gccagccgca tcgcgcacac ggtgatgacg gagatcgtcc
      661 agcagtccga cgccggcgtg gacctgcaca cggccgcgaa ccggcgcatg aacctgccgc
      721 aggtgcgcac cagcctggcc gacccgcgtg cgcgtgacct ggcgcgcgcg ttcggcgccc
      781 cgttcgtgat cgacgccagc atccgccccg gctccctgcg cgacgcggcg gaccgcgtgg
      841 gcgtgccggt gctcacctac gagggcgggc aggcactgcg cttcgaggac gacgtcatcg
      901 ccgtgggcgt ggccggcgtg ctgcgcgtca tgggcgcgct ggggatgctg gaccacgccc
      961 cgccgcaggg cgacggcgac gtggtcgagt gcgacgagac gcactggatc cgggccgacc
     1021 acggcggcat cctcgacctc cacgtcgccc cgggtgacct ggtcatggaa ggccagccgc
     1081 tgtggaccgt caccggcccg ttcggccgcg agcgcagcca gaaggtgagc ccgtacgagg
     1141 gctacgtgat cggcatgacc acgctgccgc tggtcaaccc cggggatgcc gtgctgcaca
     1201 tcgcggtccc tggcgtgcgc ggggcggcgt ccatcgacga gccgacggac gaagaggacc
     1261 tgctgggcgt ggagtccggc gagggcgacg aggagacgga ctgacatggc cggacgcgct
     1321 gagccctggc tgacgcccca gcgcctgctg ggcctggtgc tgaccctggt cggcgcgtgg
     1381 gtggccctgc aggtcgtgcg cgccttgcgc gacgtgctca tcatgctgct ggtggcgctg
     1441 ttcctgtcct tcgccatgga gccggcggtc cagcacctgg cacggcgagg ctggcggcgg
     1501 ggagccgcga ccggggcggt cttcgcggtc ttcgccctgg tgggcatgct ggtgctcgcg
     1561 gccatgacgc cgctggtgat cacgcaggtc aacggcctca tcgagagcgc gccgcgctcg
     1621 gtgaacgagg tcatcgaggc cgccaaccgc ctgccgttcg tgaacctcga agccacgccg
     1681 gaactgcgcc aggagatcga ggacttcggc gagcgtttcg gcgagcggct cagcagcgcg
     1741 gcgctcagcg ccgccggcga cgtggtgagc gtgggcggcg ccgcgatcgg cctggtgatc
     1801 caggtgctgg ccatcgggct ggtgaccttc tacctggtgg cggacggccc gcgcttccgc
     1861 cggacgctgg cgcgcccgct ccagccggcg cgccagcgcg agatgctcgc gatctgggag
     1921 atggccgtcg ccaagacggg cggctacatc tacagccggg tgctgctggc ggtgatcgcc
     1981 gcggtggtgc acgcggtcgc cctgctggtg ctgggcgtgc cgttcccgct gccgctgggc
     2041 atctggat
//
LOCUS       DATMKC010000225         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_308037, whole genome shotgun sequence.
ACCESSION   DATMKC010000225 DATMKC010000000
VERSION     DATMKC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308037"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..346
                     /locus_tag="VNU01_06530"
     CDS             101..346
                     /locus_tag="VNU01_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32311.1"
                     /translation="MAPPVESIDDYTPPAAAPEAGGIVDQVGGTVVQVIRAPGDALRL
                     LSRDPKQAAAWMAALALFCLAVMAVRRRQQAMALLEN"
     gene            497..1561
                     /locus_tag="VNU01_06535"
     CDS             497..1561
                     /locus_tag="VNU01_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HWH32312.1"
                     /translation="MTMGGAALAARHEVSGADPTGSAQARVWVDYAPGSTKVGHQVDM
                     RPAQPGAPDGGGSPTDSLGQYGSFVESLIDTLLPVSPDPEAWYVPAAEPPTVDAGPHL
                     PDLAPLPAWSIYLDESIVGTTAGDFALATAAGEDPFGGSQRAIRFGATIANHGRHSLE
                     VVGVPQPTGDEDHVRVDARQCVRFAGARVAGGERTCVEYKQVGSLVFHVQHGHFHIDG
                     FAQYRLLKDAGGRPDERAVVSRSEKVGFCMGDTDWLGKGELVVDHGWYRECRHTAPNV
                     PVTVRQGVSPQWGDSYGPGLPGQHLVIEGVPDGVYWVAITVNPPNVPGAVSLFETNRG
                     NNTSYRKVQLLKGGTEVKVL"
     gene            1659..2237
                     /locus_tag="VNU01_06540"
     CDS             1659..2237
                     /locus_tag="VNU01_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32313.1"
                     /translation="MKRVGIAALLALLLVTVGCGARVEPGPAVALQETAAPVDGDGVT
                     DPSQAGPLDGTAADGQGATSAPVEAQSSAPPPPQPGASPDYGPKHNNGVVRIELSATC
                     VKPGDLLVITFRTPPKAGLGMIIGYSDNQPHGAMLTGESDANGVYVWRVPVDPTVPDG
                     QATVLVTSTGANWEQEGGGTADAKFQVARGGC"
     gene            2280..2939
                     /locus_tag="VNU01_06545"
     CDS             2280..2939
                     /locus_tag="VNU01_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32314.1"
                     /translation="MEKRWRGRAARVAAGALAAMLLAACGGGTAGGDDHSPTATAEET
                     EAAANSQVDKSRTYRAKVHAEGTGAAPFTWDGEQEVVLTRVGGPDIDVNLLSAGFAVP
                     EIVTDDPAHRFRWAFDLLNAYEDQPGTFQITADPVNAQGLKSKAFLIWMKVKDEHKDK
                     EAVFEMDEVEYLKEFNQLQEPCTVEVGPNESSGTLTCTALATAEGEVAGLTVTWEEIP
                     G"
BASE COUNT          480 a         1035 c         1198 g          440 t
ORIGIN      
        1 gcgtcaccag gcgcccggat cacctgcacc accgtgccac cgacctggtc gacgatgccg
       61 ccagcctccg gcgccgatcg actacagccc gccggtgggt gtggcaccgc cggtggagtc
      121 gatcgatgac tacacgccgc cggccgcggc gccggaggct ggcggcatcg tcgaccaggt
      181 cggtggcacg gtggtgcagg tgatccgggc gcctggtgac gcgttgcgcc tgctgtcccg
      241 tgacccgaag caggcggcgg cgtggatggc tgcactggcg ctgttctgcc tggcggtcat
      301 ggcggtcaga cggcgtcagc aggccatggc gctcctggaa aactgacgga ccaccgtcag
      361 cgaaggctcc ggttgtccgt attgccctgt ggcggtgtca cgggtgacac tgccccaggg
      421 ccggacgtcc ggatgcgggg gttgtggagt gcgggggaat cgagttgcgg cgggtgcgct
      481 ggcgtggtgc gtcgccatga cgatgggcgg cgccgccttg gcggcgcggc acgaggtgtc
      541 gggcgccgat ccgacgggct ccgcgcaggc ccgcgtatgg gtcgactacg cgccgggcag
      601 caccaaggtg ggtcaccagg tggatatgcg gccagcccag ccgggcgcgc cggacggcgg
      661 cggctcgccg accgactcgc tggggcagta cggctccttc gtcgagtcac tcatcgacac
      721 cctgctgccg gtctccccgg atccggaggc gtggtacgtc cccgcggctg agccgccaac
      781 ggtggacgcc ggtccgcacc tgccggacct cgcaccgctc ccggcgtgga gcatctacct
      841 ggacgaatcg atcgtcggca cgacggcggg cgacttcgcc cttgcgacgg ccgccgggga
      901 ggaccccttc ggcggcagcc agagggccat ccgcttcggc gccaccatcg ccaaccacgg
      961 ccgccacagc cttgaggtgg tcggcgtgcc ccagcccacg ggcgacgagg accatgttcg
     1021 cgtggacgcc cgccagtgcg tgcgcttcgc cggggcccgt gtggccggcg gggagcgcac
     1081 ttgtgtggag tacaagcagg tcggctcgct ggtcttccat gtgcagcacg gccatttcca
     1141 catcgacggc ttcgcccagt accggctgct caaggacgcc ggtggacgcc cggacgagcg
     1201 cgcggtggtg agccggtccg agaaggtcgg cttctgcatg ggcgacacgg actggctcgg
     1261 caagggcgag ctcgtcgtgg accatggctg gtaccgggag tgccgccaca cggcgcccaa
     1321 cgtgcccgtg acggtgcgcc agggcgtctc gccgcagtgg ggtgacagct acgggcctgg
     1381 tctgccgggc cagcacctgg tcatcgaggg cgtccccgac ggcgtgtact gggtcgcgat
     1441 cacggtgaac ccgccgaacg tgccgggcgc cgtgtcgctg ttcgaaacca accggggcaa
     1501 caacacgtcc taccggaagg tgcaactgct gaagggcggc accgaggtaa aggttctcta
     1561 agcacgcgga acgtggcaaa cccgttgtgt gggacgccgt tctgccgcac actggcgcgg
     1621 cgaggcgcgg ggtctcgcga ggggtggggg gaacggacgt gaagcgggtc ggaatcgcgg
     1681 cgctcctggc gctgctgctg gtcacggtgg gttgtggcgc gcgcgtcgag ccgggccctg
     1741 ccgtggcgtt gcaggagacg gctgctcccg ttgacggcga tggtgtcacg gacccgtcgc
     1801 aggccggccc gctggacggc accgccgccg atgggcaggg cgcgacatcc gccccggtcg
     1861 aggcgcagtc ctccgcgcca ccgccaccac agccgggcgc ctcgcccgac tacggcccga
     1921 agcacaacaa cggcgtggtg cgcatcgagt tgtcggcgac ctgcgtgaag ccgggcgacc
     1981 tgctggtgat cacgttccgc acgccgccca aggccggcct cggcatgatc atcggctact
     2041 cggacaacca gccccacggc gccatgctga ccggggagtc cgacgccaac ggggtgtacg
     2101 tgtggcgagt gcccgtggac cccacggtcc cggacggcca ggccaccgtg ctggtcacgt
     2161 cgacgggtgc caattgggag caggagggcg gcggcaccgc ggacgccaag ttccaggtcg
     2221 cccgaggtgg atgctgacag cgcccgttgc gggcgacctc ttttgagaag gagcatgaga
     2281 tggaaaagcg gtggcgcggg cgcgccgcgc gagtggcggc gggtgccctg gcggcgatgc
     2341 tgctggccgc ctgcggtggc ggcacggccg gcggggacga ccacagcccg accgccacgg
     2401 ccgaggaaac ggaagcggcc gcgaatagcc aggtcgacaa gtcgcggacc taccgggcga
     2461 aggtccacgc ggaggggacg ggcgcggcgc cgttcacctg ggacggggag caggaagtcg
     2521 tgctcacccg tgtgggtggg cccgacatcg acgtgaacct gttgtcggca ggctttgcgg
     2581 tcccggagat cgtgacagac gaccccgcac accgctttcg ctgggcgttc gacctcctga
     2641 acgcctacga ggaccagccc ggcacgttcc agatcacggc cgacccggtc aacgcccaag
     2701 ggctgaagag caaggcgttc ctcatctgga tgaaggtgaa ggacgagcac aaggacaagg
     2761 aagcggtctt cgagatggac gaggtcgagt acctcaagga gttcaaccag ctccaggagc
     2821 cctgcaccgt ggaggtcggt cccaacgagt catccgggac gctcacgtgc accgcgctgg
     2881 ccacggcgga gggcgaggtc gccgggctca cggtcacctg ggaggagatc ccgggatagc
     2941 gcgatcgcgc gctggtgggg aacgctcggg cgcgagtcgg ctagactcgc gcccgacgcg
     3001 tgtccggggc tggcggaaat cgccgcggga gcgagtgaac cggacggcgt cgggcggcgt
     3061 taccaagacg ttcttagcgt aagcacgggg atcgcggtcg cgtatcacgc cgcgtgtcgg
     3121 tgagcccgga cgggatgggc tcggtgggac cgg
//
LOCUS       DATMKC010000226         3681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_297523, whole genome shotgun sequence.
ACCESSION   DATMKC010000226 DATMKC010000000
VERSION     DATMKC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297523"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VNU01_06550"
     CDS             complement(<1..294)
                     /locus_tag="VNU01_06550"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWH32315.1"
                     /translation="MNGELDLLPDAVVLVDGGGRIVRANAMAARLLGTPAGALAGRDA
                     AEAVPLADEAGMEWWSCVKPLQADARLLPRIPETDLLLTDAAGRQRPVTLTVDT"
     gene            complement(291..1139)
                     /locus_tag="VNU01_06555"
     CDS             complement(291..1139)
                     /locus_tag="VNU01_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWH32316.1"
                     /translation="MTVVTSPGNAAVKAARKLARRTERARAGEFLVESPEVVREALPH
                     LRRLFVTAEAASREAELVARAEAAGATVMVVTPEVLGGLSDTVTPQGLVGVAALPGAA
                     LDATLDEVCRAGAPLVVALSQVRDPGNAGAIIRSADAGGADVVVLTRGSVDPGNPKAV
                     RASAGSVFHLPVVTDADEEALLAACRARGLQVVAAAPRGAVPFTDVDFTRPTAVLFGN
                     EAHGLDDAVVEACDVAAAAPIHAGARPGWTGSAESLNLAATVAVFVYEAARQRHGRGG
                     GRAGAA"
     gene            complement(1147..1503)
                     /gene="rplT"
                     /locus_tag="VNU01_06560"
     CDS             complement(1147..1503)
                     /gene="rplT"
                     /locus_tag="VNU01_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWH32317.1"
                     /translation="MARVKRGVHARKQRRAVLERAKGFRGARSRRYRIANESVLHAMR
                     YQYRDRRARKGDFRKLWITRINAAARQDGLSYSRFINGLRRAEVEVDRKVLADLAVND
                     PAAFRELVAVAKSALG"
     gene            complement(1540..1743)
                     /gene="rpmI"
                     /locus_tag="VNU01_06565"
     CDS             complement(1540..1743)
                     /gene="rpmI"
                     /locus_tag="VNU01_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWH32318.1"
                     /translation="MPKQKTHSGTKDRFRVTKTGKVLHERMNMNHILEKKSSKRKRRL
                     SQEGELTGGDAKQIKKLLRGYNG"
     gene            complement(1757..2641)
                     /gene="infC"
                     /locus_tag="VNU01_06570"
     CDS             complement(1757..2641)
                     /gene="infC"
                     /locus_tag="VNU01_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWH32319.1"
                     /translation="MSDKWRRPASHLSGQPDQIRTALGQVGAQTRSRCAPPVVVRRRE
                     TGRRVTRERPSCCPQRVSAGPVAPPAHQEGTSIERDARINEQIRVPEVRLIGEDGEQL
                     GVKPLAEALRLARDLSLDLVEVAPNAAPPVCRVMDYGKFKYQQDVKAKEARKRQSHIV
                     IKEMKMRPKIDKHDYATKSRHVERFLREGSKVKATIMFRGREMAHAELGRKILDRLAD
                     DMAELAYVESAPNQDGRNMIMVLAPHKHVVEAARSGEDGEAAQTPDETPDETPDETPD
                     ETADQADVQVEDQAEATS"
     gene            complement(2793..3281)
                     /locus_tag="VNU01_06575"
     CDS             complement(2793..3281)
                     /locus_tag="VNU01_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32320.1"
                     /translation="MSDLHSAFGAEPGHGQQPSEEELRAYLMELRQADPAEFLMQAVN
                     LLVTGAQAKIGMADGRFLIDALAGAVSAVQDRLPPQLAEALQNAVVQLQTAQVQAERE
                     LAAMQGEPIPPAQDGGSAQAQEGGGPDGGAPQAGGPTMSEPQKRMTDRLWVPGKGAPP
                     VR"
     gene            complement(3330..>3681)
                     /locus_tag="VNU01_06580"
     CDS             complement(3330..>3681)
                     /locus_tag="VNU01_06580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWH32321.1"
                     /translation="LEGDAAAALRVVLGGAAAGGALLALALATPRGMGMGDVKLAAFI
                     GAGLGALGWAEVVVGVFGGFLIGGVAGIGLLATKLRRRSDHIPFGPYLAAGAFVSLLA
                     GGELAGAYLRSWGL"
BASE COUNT          414 a         1384 c         1321 g          562 t
ORIGIN      
        1 ggtgtcgacg gtcagcgtca cggggcgctg ccgcccggcc gcgtcggtga gcagcaggtc
       61 cgtctccggg atgcgcggca gcagccgggc gtcggcctgg agcggtttga cgcagctcca
      121 ccactccatg ccggcctcgt cggccagggg caccgcctcg gcggcgtcgc ggccggccag
      181 cgcgccggcg ggcgtgccca gcaggcgcgc cgccatcgcg ttggcccgca cgatgcgccc
      241 gccgccgtcg accagcacca ccgcgtcggg caggaggtcc agctcaccgt tcacgccgcc
      301 ccagcccttc cgccgccccg gccatggcgc tgcctcgccg cctcgtacac gaacaccgca
      361 accgtagcgg cgaggttgag cgactcggcg gaccccgtcc acccgggccg cgcgcccgcg
      421 tggatgggcg ccgcggccgc cacgtcgcag gcctcgacca cggcgtcgtc caggccgtgc
      481 gcctcgttgc cgaacagcac cgccgtgggg cgggtgaagt cgacgtcggt gaacggcacc
      541 gcgccgcgcg gcgcggcggc gaccacctgg aggccgcgcg cccggcacgc ggcgagcagc
      601 gcctcctcgt ccgcgtcggt gacgacgggc aggtggaaca ccgagccggc cgaggcgcgc
      661 accgccttgg ggttgccggg gtcgacgctg ccccgcgtga gcacgaccac gtcggcgccg
      721 cccgcgtcgg ccgagcggat gatcgccccg gcgttgccgg ggtcgcgcac ctgcgacagc
      781 gccacgacca gcggcgcgcc cgcgcggcac acctcgtcca gcgtggcgtc cagggcggcg
      841 cccggcagcg ccgcgacgcc cacgagcccc tgcggggtca ccgtgtcgga cagcccgccc
      901 agcacctccg gcgtgaccac catgacggtg gcccccgccg cctcggcgcg cgccaccagc
      961 tccgcctcgc gggaggcggc ctctgcggtc acgaagagcc tgcgcaggtg gggcagcgcc
     1021 tcgcgcacca cctccgggct ctccaccagg aactcgcccg ccctggcgcg ctcggtgcgc
     1081 cgggcaagct tgcgcgcggc cttcacggcc gcgttgcccg ggctcgtgac gaccgtcacg
     1141 gcgcgctcag ccgagcgcgc tcttcgcgac ggcgaccagc tcgcggaacg ccgccgggtc
     1201 gttcaccgcc aggtcggcca ggaccttgcg gtcgacctcg acctcggccc tgcgcagccc
     1261 gttgatgaag cggctgtagg acaggccgtc ctgccgcgcc gccgcgttga tgcgggtgat
     1321 ccagagcttg cggaagtcgc ccttgcgcgc gcgacggtcg cggtactggt accgcatcgc
     1381 gtgcaggacg gactcgttgg cgatccggta ccggcggctg cgggcgccgc ggaagccctt
     1441 ggcgcgctcg aggacggcgc gccgctgctt gcgtgcgtgg actccacgct tcacgcgtgc
     1501 catgaatcgt tgctccttgt tcgtgcggga ggggcagggt cagccgttgt agccgcgcag
     1561 cagcttcttg atctgcttgg cgtctccacc ggtcagctcg ccctcctggg acaggcggcg
     1621 cttccgcttg ctgctcttct tctccaggat gtggttcatg ttcatccgct cgtgaaggac
     1681 cttgccggtc ttcgtcacgc ggaagcggtc cttggtcccg gagtgcgtct tctgcttggg
     1741 catcggatcg ttcctgtcag ctggtggctt cggcctggtc ttcgacctgc acgtcggcct
     1801 ggtctgcggt ctcgtctggg gtctcgtccg gggtctcgtc tggggtctcg tccggcgtct
     1861 gggccgcctc gccgtcctcg ccgctgcgcg ccgcctcgac gacgtgcttg tgcggggcga
     1921 gcaccatgat catgttgcgg ccgtcctggt tcggcgcgct ctcgacgtag gcgagctcgg
     1981 ccatgtcgtc cgccaggcgg tcgaggatct tgcggcccag ctccgcgtgg gccatctcgc
     2041 gcccgcggaa catgatggtc gccttcacct tgctgccctc gcgcaggaag cgctcgacgt
     2101 ggcgcgactt cgtggcgtag tcgtgcttgt cgatcttggg ccgcatcttc atctctttga
     2161 tgacgatgtg gctctggcgc ttgcgcgcct ccttcgcctt gacgtcctgc tggtacttga
     2221 acttgccgta gtccatgacc cggcagaccg gcggagccgc gtttggcgcg acttcaacga
     2281 ggtccaggga cagatcgcgc gccaggcgca gcgcctccgc cagaggcttc acgccaagct
     2341 gctcgccgtc ctccccgatg aggcggactt cgggcacacg gatctgctcg ttgatgcggg
     2401 cgtctcgctc gatgctggtt ccctcctggt gtgcgggtgg cgcgaccgga ccggcggaga
     2461 cccgctgcgg gcaacacgaa ggccgctcgc gagtgacgcg gcggcctgtt tccctccggc
     2521 gcacgacgac gggcggagcg cacctgctcc gcgtctgggc gccgacctgt ccgagtgcgg
     2581 tccggatctg gtccggctgc cccgacaggt gggaggctgg ccgcctccac ttgtccgaca
     2641 tgcgattgtt cgcgttccgt gagcgggcct cgccgacggt cgcggtccac gaagtgtagc
     2701 ggggcgccac cacgggcgcg accgcccccg tttccccgtt cgaacgggtg cgggcaccgc
     2761 ggcatttcgc ggtcactgca cccgttcgcg gctcagcgga cgggtggcgc gcccttgccc
     2821 ggcacccaca gccggtcggt catgcgtttc tgcggctcgc tcatggtggg gccgccggcc
     2881 tgcggggcgc cgccatcggg accgccgccc tcctgcgcct gggcgctgcc gccgtcctgg
     2941 gccggcggga tcggctcgcc ctgcatggcg gcgagctcgc gctcggcctg cacctgcgcg
     3001 gtctgcagct ggacgaccgc gttctgcagc gcctcggcca gctggggggg cagccggtcc
     3061 tggacggcgc tgaccgctcc ggccagcgcg tcgatgagga agcggccgtc ggccatgccg
     3121 atcttggcct gcgcgccggt gacgagcagg ttcaccgcct gcatcaggaa ctcggccggg
     3181 tcggcctggc gcagctccat caggtaggcc cgcagctcct cctcggaggg ctgctggccg
     3241 tggccgggtt ctgcgccgaa ggcgctgtgg aggtcgctca tggtggtcct tgtcgtgggt
     3301 tgcggcgcgc ccatcctagg cgcctccggc tacagccccc aggagcgcag gtacgcgccg
     3361 gccagctcgc cgccggccag cagcgagacg aacgcgcccg cggcgagata gggcccgaag
     3421 gggatgtggt cgctgcgccg gcggagcttg gtggccagca ggccgatgcc ggccacgccg
     3481 ccgatgagga agccgccgaa cacgccgacg accacttcgg cccaccccag cgcccccagg
     3541 ccggcaccga tgaaggcggc gagcttcacg tcccccatgc ccatgccgcg gggagtggcc
     3601 agcgccagcg ccagcagggc cccgccggcg gcggcacccc ccaggaccac gcgcagcgcc
     3661 gcggccgcgt caccctccag g
//
LOCUS       DATMKC010000227         3733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_287465, whole genome shotgun sequence.
ACCESSION   DATMKC010000227 DATMKC010000000
VERSION     DATMKC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287465"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VNU01_06585"
     CDS             <1..480
                     /locus_tag="VNU01_06585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH32322.1"
                     /translation="LLDATVEETGAVAFAGVLTANVMPAFLFSQAVFPPMLAQGGGRI
                     VSISSCLSQRPSRRYVAYSSAKAALETFTRGLAVEWGPHGIRANIVAPGQVRTALNGP
                     ALDKPGVEERVSAKIPAKRIGRPSDVAHAVLYLSGQSSDFVNGAVLSVDGGYSVRAP"
     gene            526..2145
                     /locus_tag="VNU01_06590"
     CDS             526..2145
                     /locus_tag="VNU01_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH32323.1"
                     /translation="MELVGNNNPADRPIGRALAVQAEAIGDKVFVRTQDEAHTFAEVN
                     ARACRLANSMRDLGIKRGDPVAILMEDGYDFVSTVLAVNKAGGIWVPINTNYRGDWLS
                     RTLQDAAAAMIVADDAYLAKEVIEQAEHSGARLVVRKTASGAAAAEGRPGATTPFDAL
                     EDNVDHEIAEPLAESDISAVMWTSGTTGRSKGVMQAYGCWYGALRLYTEARDVRPDDV
                     FYNCLPMFNSGAWVFNILEALYTGTTVAIDPVFSVSRFWERVQHYGATQIATLGAMHM
                     YLWQQPPQPAEIDNPARVATAIPVPPEIHDAFKERFGLEHIVRLWGQSELMPLSINSP
                     ERQGALDADGFVRDDFDVMLLDEHDNEVAAGEVGEICVRPRRPFTMFQGYYRNPQATV
                     DAWRNLWYHSGDLGTISESGELRFVDRKADYIRYKGRNISSIELEGVVMEHPDVVEAA
                     AHGVPSSELLREDEIKVCVVRKLGAEVSEADLATHVNERAPYFLVPRYIEFVDELPHT
                     PTGRVRKVELRERGVTSTTWDRVAAGFTVQR"
     gene            2252..3421
                     /locus_tag="VNU01_06595"
     CDS             2252..3421
                     /locus_tag="VNU01_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32324.1"
                     /translation="MEFAETEEQRRFRQHVREWLDTSLERLYAGPVPPYAGAAMPIEL
                     RREWQRILHDGGWTGLHWAAEYGGRGLTLFEQQIFYDECGRARAPEPLNVIGLYMVGP
                     TIIDWGTEAQKHRYLSRILSGEDIWCQGFSEPEAGSDLASVSTRAVRQGDAYVVNGRK
                     IWTSYGSIARYCLLLVRTSEEDVRHRGLTCLLLDMSSPGVSVRPITQITGRSEFAELV
                     FDDVVVPVEDRIGAEGEGWRVVITTLLHERGTMPFALHAGSKMLCDELLELLRAAGLA
                     GDPAITDALVDLDIELESLRLANLLAIPRMLVSGPSAIDAVRKLIWERLEQHASHIAM
                     EAMGAEFVARDHEQPRAAVWSYHYLRNRGRTIEAGTTEVLKNSLAERVLGLPRVR"
     gene            3427..>3733
                     /locus_tag="VNU01_06600"
     CDS             3427..>3733
                     /locus_tag="VNU01_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32325.1"
                     /translation="MRLEVTEEQHHLRQSLHACVSKRNGAGWPAGSPAAGGDIPHDAG
                     LYRELMSLGLADVTDERDRGVLFEEAGRWILPAPVLNVAGLAVPVLRHLAPDAGVLDQ
                     "
BASE COUNT          584 a         1266 c         1326 g          557 t
ORIGIN      
        1 ctcctggacg ccacggtcga ggagaccggc gcggtcgctt tcgccggggt gctcacggcg
       61 aacgtgatgc ctgcgttcct cttttcgcag gcggtcttcc cccccatgct ggcgcagggc
      121 ggcgggcgca tcgtcagcat ctcgtcctgc ctgtcccagc ggcccagccg ccggtacgtg
      181 gcctactcca gcgcgaaggc ggcgctggag acgttcacgc gcggtcttgc ggtggaatgg
      241 gggccgcacg gcatccgggc caacatcgtg gcgcccggac aggtacgcac cgcgctcaac
      301 ggccccgcgc tcgacaagcc gggtgtggag gaacgggtgt ccgcgaagat cccggccaag
      361 cggatcggcc gcccgagcga cgtggcgcac gccgtgctgt acctgtcggg gcagtccagc
      421 gacttcgtga atggcgcggt gctcagcgtg gacggcggtt attccgtccg cgctccctga
      481 gctggcccgc cggggcaccg atcgcgcgcg agcaaaggaa ctcggatgga gctggtaggc
      541 aacaacaatc ctgcggatcg gcccatcggg cgggccctgg ccgtgcaggc ggaggcgatc
      601 ggcgacaagg tcttcgtgcg cacccaggac gaagcgcaca cgtttgccga ggtgaacgcg
      661 cgggcgtgcc ggctcgccaa ctcgatgcgc gatctgggca tcaagcgggg tgatccggta
      721 gccatcctca tggaggacgg ctacgacttt gtgtccaccg tgctggccgt caacaaggcg
      781 ggcggcatct gggtgccgat caacaccaac taccgcggcg actggctgag ccgcacgctg
      841 caggacgcgg ccgccgcgat gatcgtggcc gacgacgcct acctggcgaa agaggtcatc
      901 gagcaggccg agcacagcgg cgccaggctg gtggtgcgca agacggcctc cggggcggcc
      961 gccgccgagg gccggccggg cgcaacgacg ccgttcgacg cgctggagga caacgtcgat
     1021 cacgagattg ccgagccgct cgccgagagc gacatctccg cggtgatgtg gacatctggc
     1081 acgaccgggc ggtccaaggg cgtcatgcag gcgtacggct gctggtatgg cgcgctcagg
     1141 ctgtacaccg aggcgcgcga cgtgcggccg gacgacgtgt tctacaactg cctgccgatg
     1201 ttcaactccg gcgcctgggt gttcaacatc ctggaggcgc tgtacaccgg cacgaccgtg
     1261 gccatcgacc cggtgttctc cgtgagccgc ttctgggagc gcgtccagca ctacggggcc
     1321 acgcagatcg cgacgctggg cgccatgcac atgtacctgt ggcagcagcc gccccagccg
     1381 gcggagatcg acaatcccgc gcgggtcgcc accgcgatcc ccgtgccccc tgagatccac
     1441 gacgccttca aggagcgctt cggcctggag cacatcgtgc ggctgtgggg ccagtccgag
     1501 ctcatgccgc tgtcgatcaa ctctccggag cgccaaggag ccctggatgc cgacggcttc
     1561 gtccgtgacg acttcgacgt gatgctgttg gacgagcacg acaacgaggt ggccgccggc
     1621 gaggtggggg agatctgcgt gcgtccccgc cgcccgttca cgatgttcca gggctactac
     1681 cgcaaccccc aggccacggt cgatgcctgg cgcaacctct ggtaccactc cggagacctg
     1741 ggcacgatca gcgaatcggg cgagctgcgc ttcgtggacc gcaaggcgga ctacatccgc
     1801 tacaagggcc gcaacatctc ctccatcgag ctggagggcg tggtcatgga gcaccccgac
     1861 gtcgtggagg ccgcggctca tggggtgccg tcctcggagc tcctccgcga ggacgagatc
     1921 aaggtctgcg tcgtcaggaa gctcggcgcc gaggtgtccg aggcggacct ggccacccac
     1981 gtgaacgagc gggcgcccta cttcctggtc ccgcgctaca tcgagttcgt cgacgagctg
     2041 ccccacacgc ccacgggccg cgtgcgaaag gtggagcttc gggagcgggg cgtcaccagc
     2101 acgacctggg accgggtggc cgcggggttc accgtccagc gctgagcacg ccgggacggg
     2161 ccccggcttg accccccggg gcccctgccc tacgatctac ctatcgatcg ataagtacgg
     2221 tcggtgcaga ccgccggact ggagcacccc attggagttc gccgagacgg aggagcaacg
     2281 gcgcttccgg cagcacgttc gcgagtggct ggacaccagc ctcgagcgtc tctatgccgg
     2341 ccccgtcccg ccctacgccg gtgcggcgat gccgatcgag ctgcgccgcg agtggcagcg
     2401 catcctgcac gacggcggct ggaccgggct gcactgggcc gctgaatacg gcggccgtgg
     2461 cctgaccctg ttcgagcagc agatcttcta cgacgagtgc ggtcgtgccc gcgcccccga
     2521 gcccctgaac gtgatcgggc tgtacatggt cggccccacg atcatcgact ggggcaccga
     2581 ggcgcagaag caccgctacc tgtcgcgcat cctgtcgggg gaggacatct ggtgccaggg
     2641 cttcagcgag cccgaggccg gcagcgacct cgcctccgtc agcacccggg ccgtgcgcca
     2701 gggcgacgcg tacgtcgtga acgggcgcaa gatctggacg agctacggca gcatcgcgcg
     2761 ctactgcctc ttgctggtgc gcacgtccga ggaggacgtg cgccaccgcg gtctgacgtg
     2821 cctgctgctg gacatgtcca gccccggcgt gagcgtgcgg ccgatcacgc agatcaccgg
     2881 ccgatccgag ttcgccgagc tcgtcttcga cgacgtggtc gtgccggtgg aggaccgcat
     2941 tggcgccgag ggcgaaggct ggcgcgtggt gatcaccacg ctgctgcacg agcgcggcac
     3001 gatgccgttc gccctgcacg cgggcagcaa gatgctctgt gacgaactgc tggaactgct
     3061 gcgcgccgcc gggctggctg gcgatcccgc cataaccgac gcgctggtcg acctcgacat
     3121 cgagctggag tcgctgcggt tggccaacct gctggccatc ccgcgcatgc tcgtgtccgg
     3181 tcccagcgcg atcgacgccg tgcgcaaact catctgggag cggctcgagc agcatgcctc
     3241 gcacatcgcg atggaggcga tgggcgccga gttcgtcgct cgggatcacg aacagcctag
     3301 ggcagcagtg tggagctacc actacctgcg caaccggggg agaacgatcg aggccggcac
     3361 caccgaggtc ctcaagaact cgcttgccga gcgtgtgctc ggcctgccga gggtgaggtg
     3421 accggcgtgc gtctggaggt gaccgaggag cagcaccatc tgcgccagtc gctgcacgcg
     3481 tgcgtgtcaa agcggaacgg cgccggctgg ccggccggct caccggcggc gggtggggac
     3541 atcccccatg atgccgggct gtaccgcgag ctgatgtccc tggggctggc cgacgtgacg
     3601 gatgagcgcg accgcggcgt gctgttcgag gaggcgggac gctggatcct gcccgcaccg
     3661 gtgctcaacg tggcggggct ggccgtgccg gtgctgcgcc acctggcgcc cgatgccggc
     3721 gtgctggacc agc
//
LOCUS       DATMKC010000228        12822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_73161, whole genome shotgun sequence.
ACCESSION   DATMKC010000228 DATMKC010000000
VERSION     DATMKC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12822
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73161"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VNU01_06605"
     CDS             complement(<1..296)
                     /locus_tag="VNU01_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32326.1"
                     /translation="MTPQEAVAATKDALLFLGRAYMIDPATYQRGQEHGFAGFDFYAA
                     GRFGVLGPVDADVVAAGAVFLHPDGLRAAWEAGLAVMPVDQAVACWAEARRDWGR"
     gene            complement(293..1756)
                     /locus_tag="VNU01_06610"
     CDS             complement(293..1756)
                     /locus_tag="VNU01_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWH32327.1"
                     /translation="MGEPTGFLKWGRETPTRRPVPLRVQDWHEVYEPFPDEKLQVQAG
                     RCMDCGIPFCNMGCPLGNLIPDWNDLVYRDRWREAIERLHATNNFPEFTGRLCPAPCE
                     AACVLGINQPAVTIKQVEVEIIEHAFGEGWVVPLPPQAETGKRVAVVGSGPAGLAAAQ
                     QLRRAGHAVTVFERADRIGGLLRYGIPEFKMEKRFLDRRLAQMEAEGIAFRVNANVGV
                     DVPVEELRRDYDAIVLAGGATQARDLPIPGRELAGVYQAMEYLPWANRVAIGELERSP
                     IDVAGKKVVIIGGGDTGADCLGTAHRQGAASVHQFEIMPRPPEERSDAMPWPTYPMTY
                     RVSSAHEEGGERVYSVNTERFLGDEQGNLRALRAHQVEQVVEDGRMRFDKVEGTDFEL
                     ECDFVFLAMGFTGPEREGMLEQLGVELDARGNVARDDDWHTSVDGVFVCGDMGRGQSL
                     IVWAISEGRACAQAVDRRLMGRTLLPAAIAPTARPIG"
     gene            complement(1749..6338)
                     /gene="gltB"
                     /locus_tag="VNU01_06615"
     CDS             complement(1749..6338)
                     /gene="gltB"
                     /locus_tag="VNU01_06615"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWH32328.1"
                     /translation="MRQTPVSQPGPQRGALGLYRPEFEHDACGVSFVVDLKGRRSHDI
                     VSQGVTALCNLEHRGASGAEVNTGDGAGILIQVPDRFLREVAGFALPEAGAYAVGTAF
                     LPADATEAKAAAAAIEKLAADEGVTVLGWRDVPIDDSMIGATAKSVQPLFRQLFVAAQ
                     SPDGSARAGVDLDRAIYPFRKRVEHEVTGVYFASLSSRTLVYKGMLTTPQLPAFFADL
                     TDERVESALALVHSRFSTNTFPSWPLAHPYRFVAHNGEINTLQGNRNWMRAREALLAT
                     DLIPGDLKRCFPIVTPGASDSASFDEVLELLHLGGRPLPHAVLMMIPEAWENHAQMPA
                     ERKAFYEFHASLMEPWDGPASIAFTDGTVIGAVLDRNGLRPSRYWVTADDRVIMASEV
                     GVLDVEPSQVVRKGRLQPGRMFLVDTSQGRIIGDEEIKAELASEHPYAEWLERGLVGL
                     EELPERRVLLPQHASVVQHQRLFGYSTEELRIIVQPMAERGAEPIGSMGSDTPIAVLS
                     NRSRLLYDYFTQLFAQVTNPPLDAIREELVTSLRQRLGPEGNLLDATPAHASQIELPF
                     PVLSNEDLAKLRYIDEDVPDSDVARRAAPFRSFTIDGLFPVARGGQGMRQAIEDVRAK
                     VSEAIADGANIIVLSDRFATPELAPIPALLLTSAVHHHLIREKTRTQVGLVVETGEAR
                     EVHHMALLIGYGAAAVNPYLAFETIDDLIRDGVIALPREKAIANYIKAAGKGVLKVMS
                     KMGISTVASYTGAQVFEAIGLAQDVVDEYFTGTVSRLGGVGLDVLAEEVALRHRKAFP
                     DRPTELAHRELEIGGEYQWRREGEFHLFNPETVFKLQHATRAGRYDIFRQYTRMVDEQ
                     SQRLGTLRGLFRFKDGARPPVPIDEVEPVSEIVKRFSTGAMSYGSISAEAHETLAIAM
                     NRLGAKSNTGEGGEDPDRFTPDANGDLRRSAIKQVASGRFGVTSEYLVNSDDIQIKMA
                     QGAKPGEGGQLPGSKVYPWIAKTRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNAN
                     NSARIHVKLVAEVGVGTVAAGVSKAHADVVLISGHDGGTGASPLTSLKHAGAPWELGL
                     AETQQTLLLNGLRDRIVVQTDGQLKTGRDVVIAALLGAEEYGFATAPLVVSGCIMMRV
                     CHLDTCPVGVATQNPELRKRFTGKPEFVVNFFTYIAEEVRELLAELGFRSLDEAIGHA
                     ELLDVRDAVDHWKASGLDLSPILTLPDLPEGTPLHCVTTQDHGLDKALDNELIAQARP
                     ALEDAAPVRFASPIRNVNRTVGTMLGAEVTRRYGGKGLPDDTIQIDFTGSAGQSFGAF
                     VPAGMTLRLEGDANDYVGKGLSGGRLVLRPHRDAPLVAEENIIAGNVCLYGATSGEAF
                     FRGVVGERFCVRNSGATAVVEGVGDHGCEYMTGGRVVVLGATGRNFGAGMSGGIAYVY
                     DADGTFPPLVNPEMVDLDPLDAEDRAFLNEIVERHQRETGSAVAERLLADWDTSVERF
                     VKVMPSDYKRVLEAMRTAEEKGMSVDEAVMAAAHG"
     gene            6463..7437
                     /locus_tag="VNU01_06620"
     CDS             6463..7437
                     /locus_tag="VNU01_06620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWH32329.1"
                     /translation="MVEVGYTLSSEDHGPRDLVRFAGLAEEHGFTRAWISDHFHPWVD
                     AQGHSPFVWAVLGGVAATTGLEVGTGVTCPTVRIHPAVVAQAVATTSLLFDGRFWFGV
                     GSGEALNEHILGGRWPEAEVRLEMLEEAVQVMRRLWTGEVVSHHGRHYTVENARLYDA
                     PERRVPVYVSAFGPKAMELAGRIGDGYVGTSPDAELVRRFARQAPGKPKVAGAKCCWH
                     ADEAEARRLAHRLWPNMGLPGELAQELPTPQHFMQAARLVTEEMVAGPIPTGPDPRPY
                     VDLVRAYADAGYDVVYLHNIGEDQEGFLRFWTGEVRPLLAEEGLTRAA"
     gene            complement(7434..8438)
                     /locus_tag="VNU01_06625"
     CDS             complement(7434..8438)
                     /locus_tag="VNU01_06625"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWH32330.1"
                     /translation="MPAVTVDNALALPAVSRPDPTHRSERRVVSVTSAPSAFEGEGFP
                     VKRAFAGVDLALLDPFIHLDQMGEVEYAPGEPKGTPWHPHRGFETVTYMIDGVMEHAD
                     THGGGGKITDGDTQWMTAGSGLLHIETPPEWLVAKGGLFHGFQLWVNLPAAMKWTEPR
                     YQDIRGSEVTLLRSHDGGALLRVIAGEVDGHSGPGSTHTPITLVHATLEPGARLELPW
                     RGDFNALAYVLNGRGHVGANRRGIEPHQLAVFGVDGDHLVITADERQEGRSPRMDVLI
                     LGGRPIKEPVAWYGPFVMNTRAEVFQAVEDFHAGKLGVIPATGRMPHTAPGETVESTD
                     "
     gene            8604..9065
                     /locus_tag="VNU01_06630"
     CDS             8604..9065
                     /locus_tag="VNU01_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32331.1"
                     /translation="MPRLLVVHHTPSPSTRAMLEAVLDGTRADGIEGVEVVVRPALAA
                     TVPDALEADGYVLGTPANIGYMSGALKHWFDQVYYPAMDATARRPYALFLHGNNDTAG
                     AVKAVESIAGALGWKRVREPLEVIGEPSPADLEACWELGAAVAAALAEGLA"
     gene            complement(9062..10228)
                     /locus_tag="VNU01_06635"
     CDS             complement(9062..10228)
                     /locus_tag="VNU01_06635"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWH32332.1"
                     /translation="MTQRIYAMPTEITGWTVPGVANVQFNWEYDAGRDKLLSLYEKGK
                     NKQWNTNERIDWSIEIDLDDTSGMPDEYVPLFGSPMWDKLDRAGKNQVRHHYGAWLNS
                     QFLHGEQGALICAARIVETVPDIDAKWYGATQVMDEARHVETYARYLHEKLNLAYPIN
                     PHLKELLNQTLTDTRWDMTYLGMQIMIEGVALAAFSMIRDFTGEPLSKAINAYVMQDE
                     ARHVAFGLLALKDAYAEMTSAEKAEREEFVVEASYLLRDRFLAREVWENLGFDADECV
                     EYVNNHQMLGEFRKALFSRVVPNVKKIGLWGPKVQEAFVDMGVIQFQDMDPDESFARD
                     ESIAEELDRLMEQGRHKEIRFMWETDAEQVAETIAVGATATDEEAKSLGGSAPS"
     gene            10379..11161
                     /locus_tag="VNU01_06640"
     CDS             10379..11161
                     /locus_tag="VNU01_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH32333.1"
                     /translation="MSDVLRELFSVEGKVAVVTGGSRGIGRMIAEGFVRAGVRVYITA
                     RKAEQVEQTARELSELGECVAIPADLSSEAEANRFAAAVAEREDILHILVNNAGATWG
                     EPIETFPDSAWDKVLGVNVKGVFHLTRAFLPQLRAAGTADDPARVINIGSIDGIHVPM
                     LETYAYSASKAAVHQLTRHLAKRLAAEHVTVNAVAPGPFPSKMMQATLEAFEEAIVAG
                     CPLGRIGRAEDMAGVAIYLASRAASYVTGTVIPVDGGIATTK"
     gene            complement(11124..>12822)
                     /locus_tag="VNU01_06645"
     CDS             complement(11124..>12822)
                     /locus_tag="VNU01_06645"
                     /inference="COORDINATES: protein motif:HMM:NF023284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3376 domain-containing protein"
                     /protein_id="HWH32334.1"
                     /translation="TPNAFGAPTEIGLKLTGTRTGHFAAFYKPSWRVNDWIWGRLDAA
                     VRLAQLALDPARLRQLRYSSADALAALREAAVGPPADAGAPVRERVERDRDHLAVAFD
                     AAAQDCARELSYLDDDDLPVPPSLPVCALQVARRVQAEVLREELERLAVAAREDERAG
                     AARDSESVAFRARYEEALRGVGGREGLAAEQLFALFRESRVGAETVMDDAGSDLFAGT
                     VSRAALVGASAVEGRRSGLWPLRAVTRPIRAVLLVLHVLVAGATRGSGFGLLLVSLAL
                     AAGGALLAVSALAEEAPPLVGTVGATLAAAAVLLSALRARVWPFVVLLAGGLVTLLFL
                     LSRQQHAAAGPESTFWTVAVVVGLTWAIGMLRQPSAAPPLVRGPVWWQLALVVVGTAA
                     TVAVGLRFPVIEPCALRDLPHCGVIGLEFAGTPEGLRELVRDWDASRLADAVAHTRLD
                     FAFLALYWIPISALCALAARALGRRGRSARALGAARAAWYASWAPLVAAGLDVVENVA
                     LLRMLHEAGGPLPPIVPAVAFVAAGVKWLLVVGALLYGLVALVLAATWWWRCRRRRG"
BASE COUNT         1780 a         4600 c         4628 g         1814 t
ORIGIN      
        1 cggccccagt cccggcgcgc ctcggcccag cacgccacgg cctggtcgac cggcatgacg
       61 gccagccccg cctcccaggc ggcgcgcagc ccgtcggggt gcaaaaacac cgcgccggcg
      121 gccacgacgt cggcgtccac gggccccagc acgccgaagc gccccgcggc gtagaagtcg
      181 aagccggcga acccgtgctc ctgcccgcgc tggtacgtgg cggggtcgat catgtaggcc
      241 ctgcccagga acagcagcgc gtccttggtg gccgcgaccg cctcctgcgg cgtcatccga
      301 tggggcgggc ggtgggcgcg atggcggcgg gcagcagcgt ccgccccatg agccggcggt
      361 ccaccgcctg cgcgcacgcg cgcccttcgg aaatggccca cacgatgagc gactggccgc
      421 gacccatgtc gccgcagacg aacacgccgt ccacggaggt gtgccagtcg tcgtcgcgcg
      481 ccacgttgcc gcgcgcgtcc agctcgacgc cgagctgctc cagcatcccc tcgcgctccg
      541 gaccggtgaa gcccatggcc aggaagacga agtcgcactc cagctcgaag tcggtgccct
      601 cgaccttgtc gaagcgcatg cggccgtcct cgaccacctg ctcgacctgg tgggcgcgca
      661 gcgcccgcag gttgccctgc tcgtcgccca ggaagcgctc ggtgttcacc gagtacacgc
      721 gctcgccgcc ctcctcgtgc gcggaggaca cccggtaggt catcggatag gtgggccacg
      781 gcatggcgtc gctgcgctcc tccggggggc gcggcatgat ctcgaactgg tgcacgctgg
      841 cggcgccctg ccggtgggcg gtgcccaggc agtccgcgcc ggtgtcgccg ccgccgatga
      901 tgacgacctt cttgccggcc acgtcgatgg gcgagcgctc cagctcgccg atggcgacgc
      961 ggttggccca gggcaggtac tccatcgcct ggtagacgcc cgcgagctcg cgcccgggaa
     1021 tgggcaggtc gcgcgcctgc gtggcgccgc cggccagcac gatcgcgtcg tagtcgcggc
     1081 gcagctcttc cacgggcacg tccacgccga cgttggcgtt gacgcggaac gcgatgccct
     1141 cggcctccat ctgcgccagc cggcggtcga ggaagcgctt ctccatcttg aactcgggga
     1201 tgccgtagcg cagcaggccg ccgatgcgat ccgcgcgctc gaacaccgtc acggcgtgcc
     1261 cggcccggcg cagctgctgc gcggcggcga gcccggccgg cccggagccg accacggcga
     1321 cgcgcttgcc ggtctcggcc tgcggcggca gcgggaccac ccagccctcg ccgaaggcgt
     1381 gctcgatgat ctcgacctcg acctgcttga tggtcaccgc gggctggttg atgcccagca
     1441 cgcaggcggc ctcgcacggc gcggggcaca gccgcccggt gaactccggg aagttgttcg
     1501 tggcgtgcag ccgctcgatc gcctcgcgcc agcggtcgcg gtagaccagg tcgttccagt
     1561 ccgggatgag gttgcccagc gggcagccca tgttgcagaa cgggatgccg cagtccatgc
     1621 agcggccggc ctgcacctgg agcttctcgt cggggaacgg ctcgtagacc tcgtgccagt
     1681 cctgcacgcg caggggaacg gggcggcggg tgggcgtctc acgcccccac ttcaggaacc
     1741 cggtcggctc acccatgagc ggccgccatg actgcctcgt ccaccgacat gcccttctcc
     1801 tcagcggtgc gcatggcctc gagcacgcgc ttgtagtcgg acggcatgac cttcacgaag
     1861 cgctccaccg aggtgtccca gtcggcgagc agccgctcgg ccaccgccga gccggtctcg
     1921 cgctggtggc gctccacgat ctcgttcagg aacgcgcgat cctcggcgtc cagcgggtcc
     1981 aggtccacca tctcggggtt caccagcggc gggaacgtcc cgtcggcgtc gtacacgtag
     2041 gcgatgccgc ccgacatgcc ggcgccgaag ttgcggcccg tcgcgcccag caccaccacg
     2101 cggccgccgg tcatgtactc gcagccgtgg tcgcccacgc cctcgaccac cgcggtggcc
     2161 ccggagttgc gcacgcagaa gcgctcgccc acgacgccgc ggaagaacgc ctcgcccgac
     2221 gtggctccgt acaggcacac gttgcccgcg atgatgttct cctcggccac cagcggcgcg
     2281 tcgcggtgcg gccgcagcac cagccgcccg ccggacaggc ccttgccgac gtagtcgttg
     2341 gcgtcgccct ccaggcgcag cgtcatgccc gccggcacga acgcgccgaa gctctgcccg
     2401 gcagagccgg tgaagtcgat ctggatggtg tcgtccggca ggcccttgcc gccgtagcgc
     2461 cgggtcacct cggcgccgag catggtgccg acggtgcggt tgacgttgcg gatgggcgag
     2521 gcgaaccgca ccggtgcggc gtcctccagc gcgggccggg cctgcgcgat cagctcgttg
     2581 tccagcgcct tgtccaggcc gtggtcctgc gtggtcacgc agtgcagcgg cgtgccctcc
     2641 ggcaggtccg gcagcgtcag gatcggcgag aggtccaggc ccgaggcctt ccagtggtcc
     2701 accgcgtcgc gcacgtccag cagctcggcg tgcccgatcg cctcgtcgag gctgcggaag
     2761 ccgagctccg cgagcagttc gcggacctcc tcggcgatgt aggtgaagaa gttgacgacg
     2821 aactccggct tgccggtgaa gcgcttgcgc aactccgggt tctgcgtggc cacgcccacc
     2881 gggcaggtgt ccaggtggca cacgcgcatc atgatgcagc ccgacaccac cagcggggcc
     2941 gtggcgaagc cgtactcctc cgcccccagc agcgcggcga tcacgacgtc gcggccggtc
     3001 ttgagctggc cgtcggtctg caccacgatg cggtcgcgca gaccgttgag cagcagcgtc
     3061 tgctgcgtct ccgccaggcc cagctcccac ggcgcgcccg cgtgcttcag cgaggtgagc
     3121 ggcgaggcac cggtgccgcc gtcatggccg gagatcagca ccacgtccgc gtgcgccttg
     3181 ctcacgcccg ccgccaccgt gccgacgccg acctccgcca ccagcttcac gtggatgcgc
     3241 gcgctgttgt tggcgttctt gaggtcgtgg atgagctgcg ccaggtcctc gatggaatag
     3301 atgtcgtggt gcggcggcgg cgagatcagc cccacaccgg gcgtggagtg ccgcgtcttg
     3361 gcgatccacg ggtagacctt ggagccgggc agctggccgc cctcgccggg cttggcgccc
     3421 tgcgccatct tgatctggat gtcgtcgctg ttgaccaggt actcgctggt gacgccgaag
     3481 cggcccgagg ccacctgctt gatggcgctg cgccgcaggt cgccgttggc gtccggggtg
     3541 aagcggtccg ggtcctcgcc gccctcgccg gtgttggact tggcgcccag ccggttcatg
     3601 gcgatcgcca gggtctcgtg ggcctcggcg gagatggagc cgtagctcat cgcccccgtg
     3661 gagaagcgct tgacgatctc gctgaccggc tccacctcgt cgatgggcac cggcgggcgc
     3721 gccccgtcct tgaagcggaa cagcccgcgc agggtgccca ggcgctggga ctgctcgtcg
     3781 accatgcgcg tgtactgccg gaagatgtcg tagcggccgg cgcgcgtggc gtgctggagc
     3841 ttgaacacgg tctccgggtt gaacaggtgg aactcgccct cgcggcgcca ctggtactcg
     3901 ccgccgatct ccagctcgcg gtgcgcgagc tcggtggggc ggtcggggaa cgccttgcgg
     3961 tggcgcagcg ccacctcctc ggccagcacg tccaggccca cgccgcccag gcggctcacc
     4021 gtgccggtga agtactcgtc gacgacgtcc tgggccagcc cgatggcctc gaagacctgg
     4081 gcgccggtgt aggaggccac cgtggagatg cccatcttgg acatcacctt cagcacgccc
     4141 ttgccggccg ccttgatgta gttggcgatg gccttctcgc gcggcagcgc gatgacgccg
     4201 tcgcggatga ggtcgtcgat ggtctcgaag gcgaggtagg ggttcaccgc cgcggcgccg
     4261 tagccgatca gcagcgccat gtggtgcacc tcgcgggcct cgccggtctc caccaccagg
     4321 ccgacctgcg tgcgcgtctt ctcgcggatg aggtggtggt gcacggcgga ggtcagcagc
     4381 agcgccggga tgggcgcgag ctccggcgtg gcgaagcggt ccgacagcac gatgatgttg
     4441 gcgccgtcgg cgatcgcctc gctgaccttc gcgcgcacgt cctcgatggc ctggcgcatg
     4501 ccctggccgc cccgggcgac cgggaacagc ccgtcgatgg tgaacgaccg gaacggcgcg
     4561 gcgcggcgcg ccacgtcgct gtcgggcacg tcctcgtcga tgtagcgcag cttggccagg
     4621 tcctcgttgg acagcacggg gaacggcagc tcgatctggc tggcgtgcgc cggcgtggcg
     4681 tccaggaggt tgccctccgg gcccaggcgc tggcgcagcg aggtcaccag ctcctcgcgg
     4741 atggcgtcca gcggcgggtt ggtgacctgc gcgaagagct gcgtgaagta gtcgtacagc
     4801 aggcgcgagc ggttgctgag caccgcgatg ggcgtgtccg agcccatgga gccgatcggc
     4861 tcggcaccgc gctccgccat gggctgcacg atgatgcgca gctcctcggt gctgtagccg
     4921 aacaggcgct ggtgctgcac cacggaggcg tgctgcggca gcaggacgcg gcgctccggc
     4981 agctcctcca ggcccaccag gccccgctcc agccactcgg cgtacgggtg ctcggaggcg
     5041 agctcggcct tgatctcctc gtcgccgatg atgcggccct gcgacgtgtc gaccaggaac
     5101 atgcggcccg gctgcagccg gcccttgcgc acgacctgcg acggctccac gtccaggacg
     5161 ccgacctcgc tggccatgat cacgcggtcg tccgcggtga cccagtagcg cgacgggcgc
     5221 aggccgttgc ggtccaggac cgcgccgatg acggtgccgt cggtgaacgc gatggaggcc
     5281 gggccatccc agggctccat gagcgaggcg tggaactcgt agaaggcctt gcgctcggcg
     5341 ggcatctgcg cgtggttctc ccacgcctcc gggatcatca tcagcacggc gtgcggcagg
     5401 ggccgcccgc ccaggtgcag cagctccagc acctcgtcga aggaggcgga gtcgctggcg
     5461 cccggcgtga cgatggggaa gcagcgcttg aggtcgccgg ggatgaggtc ggtggccagc
     5521 agcgcctcgc gcgcgcgcat ccagttgcgg ttgccctgca gggtgttgat ctcgccgttg
     5581 tgcgccacga agcggtacgg gtgggccagc ggccagctcg ggaacgtgtt cgtcgagaag
     5641 cgcgagtgca ccagggccag cgcgctctcg acgcgctcgt cggtgaggtc ggcgaagaac
     5701 gccgggagct gcggcgtggt cagcatgccc ttgtagacca gcgtgcggct ggacaggctc
     5761 gcgaagtaga cgccggtgac ctcgtgctcg acgcgcttgc ggaacgggta gatcgcgcgg
     5821 tccaggtcca cgccggcgcg ggccgacccg tcgggggact gcgcggccac gaagagctgg
     5881 cggaacagcg gctgcacgct cttggcggtg gcgccgatca tcgagtcgtc gatcggcacg
     5941 tcgcgccagc ccaggacggt cacgccctcg tcggcggcca gcttctcgat cgcggcggcc
     6001 gcggccttcg cctccgtggc gtccgcgggc aggaacgcgg tgccgacggc gtaggcgccg
     6061 gcctccggca gcgcgaaccc ggcgacctcg cgcaggaagc ggtccggcac ctggatcagg
     6121 atgccggcgc cgtcgccggt gttgacctcg gcgccggacg cgccgcggtg ctccaggttg
     6181 cacagcgcgg tgacgccctg ggacacgatg tcgtggctgc ggcggccctt gaggtccacc
     6241 acgaagctga cgccgcaggc gtcgtgctcg aattcggggc ggtacagccc cagggccccc
     6301 ctctggggac cgggctggga cacaggcgtc tggcgcattc cgtcggatcc tctggccaaa
     6361 gtgacaaatg gtgctggtcg cttcgccagg gacgacgttg gccctgtcga tgtgccggga
     6421 atggtaccgg cccccgcccg cacgagccaa tgggagcgcc agatggtcga agtcggttac
     6481 acgctgtcca gcgaggatca cgggccccgc gacctggtgc gcttcgcggg cctggccgag
     6541 gagcacggct tcaccagggc ctggatctcc gaccacttcc atccctgggt ggacgcccag
     6601 ggccactccc ccttcgtctg ggcggtcctg ggcggggtgg ccgccaccac cggcctggag
     6661 gtgggcaccg gggtgacgtg ccccacggtg cgcatccacc cggccgtggt ggcgcaggcc
     6721 gtggcgacca cctcgctgct gttcgacggc cgcttctggt tcggcgtggg cagcggcgag
     6781 gcgctcaacg agcacatcct gggcggccgc tggccggagg ccgaggtccg cctggagatg
     6841 ctggaggagg ccgtccaggt catgcggcgg ctgtggacgg gcgaggtggt cagccaccac
     6901 ggccggcact acaccgtgga gaacgcgcgc ctctacgacg caccggagcg ccgggtcccg
     6961 gtgtacgtgt cggcgttcgg gcccaaggcg atggagctcg ccgggcgcat cggcgacggc
     7021 tacgtgggca cctcgccgga cgcggagctg gtgcggcggt tcgcgcgcca ggcgccaggc
     7081 aagcccaagg tcgcgggcgc gaagtgctgc tggcacgccg acgaggcgga ggcgcggcgc
     7141 ctggcgcacc ggctgtggcc gaacatgggc ctgcccgggg agctggcgca ggagctgccg
     7201 acgccgcagc acttcatgca ggcggcgcgg ctggtgaccg aggagatggt ggcgggaccg
     7261 atccccaccg gccccgatcc ccggccgtac gtggatctcg tgcgcgcgta cgccgacgcc
     7321 ggctacgacg tggtgtacct gcacaacatc ggcgaggacc aggagggctt cctgcgcttc
     7381 tggacgggcg aggtgcggcc cctgctggcc gaggagggcc tcacccgcgc ggcctagtcg
     7441 gtgctctcga cggtctcgcc gggggcggtg tgcggcatgc gccccgtggc cgggatgacg
     7501 ccgagcttgc cggcgtggaa gtcctccacg gcctggaaca cctccgcgcg cgtgttcatg
     7561 acgaacggcc cgtaccaggc caccggctcc ttgatgggcc ggccgcccag gatcagcacg
     7621 tccatgcgcg ggctgcggcc ctcctggcgc tcgtcggcgg tgatgacgag gtggtcgccg
     7681 tcgacgccga acaccgcgag ctgatggggc tcgatgccgc gccggttggc gcccacgtgg
     7741 ccgcgcccgt tgagcacgta ggccagcgcg ttgaagtcgc cgcgccacgg cagttccagg
     7801 cgcgcgcccg gctccagcgt ggcgtgcacc agcgtgatcg gcgtgtgcgt ggagcccggg
     7861 ccggagtgac cgtccacctc gcccgcgatg acgcgcagca gcgcgccgcc gtcgtgcgag
     7921 cgcagcagcg tgacctcgct gccgcggatg tcctggtagc gcggctccgt ccacttcatc
     7981 gcggcgggca ggttcaccca aagctggaag ccgtggaaca gaccgccctt ggcgaccagc
     8041 cactccggcg gcgtctcgat gtgcagcagc ccgctgcccg ccgtcatcca ctgggtgtcg
     8101 ccgtcggtga tcttgccgcc gccgccgtgg gtgtcggcgt gctccatgac gccgtcgatc
     8161 atgtacgtga cggtctcgaa gccgcggtgg gggtgccacg gcgtgccctt gggctcgccc
     8221 ggcgcgtact cgacctcgcc catctggtcc aggtggatga acgggtccag cagcgccagg
     8281 tccacaccgg cgaaggcgcg cttcacgggg aagccctcgc cctcgaaggc gctgggggcg
     8341 gacgtcaccg acaccacccg gcgctcgctg cggtgggtgg ggtcgggccg gctgacggcc
     8401 ggcagggcca gggcgttgtc tacggtcacg gcgggcatgg cgcccctcct cggtacggcc
     8461 tgagcgactg caaggatagt tgaatcaaca atcacctgcc gcccccgcat tcccaacctg
     8521 gaacgaccgt ctgacaccgc ccggacaaag gttcccggtt gggagagttg agcgggaggg
     8581 gcggcaggcg tcaggctggc gacatgccca gactcctggt ggtccaccac acgccctcgc
     8641 cctccacccg ggcgatgctg gaggccgtgc tcgacggcac gcgcgcggac ggcatcgaag
     8701 gcgtcgaggt ggtcgtgcgg ccggcgctgg cggccaccgt gcccgacgcc ttggaggccg
     8761 acggctacgt gctcggcacg ccggccaaca tcggctacat gtcgggcgcc ttgaagcact
     8821 ggttcgacca ggtctactac ccggccatgg acgccacggc gcgccgtccg tacgcgctgt
     8881 tcctgcacgg caacaacgac acggccggcg cggtgaaggc ggtcgagtcg atcgccggcg
     8941 cgctgggctg gaagcgtgtg cgcgagccgc tggaggtgat cggcgagccg tcgcccgcgg
     9001 acctggaggc ctgctgggag ctgggcgccg cggtggccgc cgcgctcgcc gaggggctgg
     9061 cctagctcgg cgcggacccg cccaggctct tggcctcctc atcggtggcg gtcgcgccga
     9121 cggcgatggt ctcggccacc tgctccgcgt cggtctccca catgaagcgg atctccttgt
     9181 ggcggccctg ctccatgagg cggtccagct cctccgcgat cgactcgtcg cgcgcgaagc
     9241 tctcgtccgg gtccatgtcc tggaactgga tgacgcccat gtccacgaag gcctcctgca
     9301 ccttcgggcc ccacaggccg atcttcttga cgttgggcac cacgcgggag aacagcgcct
     9361 tgcggaactc ccccagcatc tggtggttgt tcacgtactc cacgcactcg tcggcgtcga
     9421 agcccaggtt ctcccacacc tcgcgcgcca ggaagcggtc gcgcaacagg tagctcgcct
     9481 cgaccacgaa ctcctcgcgc tcggccttct ccgccgaggt catctccgcg taggcgtcct
     9541 tgagcgcaag caggccgaac gcgacgtggc gcgcctcgtc ctgcatcacg taggcgttga
     9601 tggccttgct cagcggctcg ccggtgaagt cgcggatcat gctgaacgcc gccagcgcca
     9661 cgccctcgat catgatctgc atgcccaggt aggtcatgtc ccagcgcgtg tcggtcagcg
     9721 tctggttcag cagctccttc aagtgcgggt tgatcggata ggccagattc agcttctcgt
     9781 ggagataccg cgcgtacgtc tcgacgtggc gcgcctcgtc catgacctgc gtggcgccgt
     9841 accacttcgc gtcgatgtcc ggcacggtct ccacgatgcg ggcggcgcag atcagcgcgc
     9901 cctgctcacc gtggaggaac tgcgagttca gccacgcgcc gtagtggtgg cgcacctggt
     9961 tcttgccggc gcggtccagc ttgtcccaca tcggcgagcc gaacagcggc acgtactcgt
    10021 cgggcatgcc gctggtgtcg tcgaggtcga tctcgatgga ccagtcgatg cgctcgttgg
    10081 tgttccactg cttgttcttg cccttctcat agagcgagag cagcttgtcg cggccggcgt
    10141 cgtactccca gttgaactgc acgttggcca cgcccggcac ggtccagccc gtgatctccg
    10201 tcggcatcgc atagatccgc tgcgtcatct cgtgcccctg tcgccagcaa ccgtcaccgc
    10261 tcgatgtgac aatgctacga acgatgtcac atcgtgtgaa ggggcatttc ggcgggcagt
    10321 tggacagccg gcgcgccgtc ggggaagctg cgcgccactg cgtgcaaagg aggcgggcgt
    10381 gtcggacgtg ttgcgggaac tgttctcggt ggagggcaag gtcgcggtgg tcaccggggg
    10441 aagccgcggg atcggccgca tgatcgcgga gggcttcgtg cgcgccggcg tgcgcgtcta
    10501 catcacggcc cgcaaggcgg agcaggtgga gcagaccgcc cgcgagctct ccgagctggg
    10561 cgagtgcgtc gccatcccgg cggacctgtc cagcgaggcc gaggccaacc gcttcgccgc
    10621 cgccgtggcc gagcgcgagg acatcctgca catcctggtc aacaacgcgg gcgccacctg
    10681 gggcgagccg atcgagacgt tccccgacag cgcctgggac aaggtgctcg gcgtcaacgt
    10741 caagggcgtg ttccacctga cgcgcgcgtt cctgccgcag ctccgcgccg cgggcaccgc
    10801 ggacgacccg gcgcgcgtga tcaacatcgg ctccatcgac ggcatccacg tgcccatgct
    10861 ggagacctac gcctactcgg cgagcaaggc ggcggtgcac cagctcacgc gccacctggc
    10921 caagcgcctg gcggccgagc acgtcaccgt gaacgccgtg gcgccggggc cgttccccag
    10981 caagatgatg caggccacgc tggaggcctt cgaggaggcc atcgtggcgg gctgcccgct
    11041 gggccgcatc ggccgggccg aggacatggc gggggtggcg atctacctcg cctcgcgcgc
    11101 cgcctcctac gtcaccggca cggtcatccc cgtcgacggc ggcatcgcca ccaccaagta
    11161 gcggcgagga cgagcgcgac cagcccgtac agcagcgcgc cgacgacgag cagccatttc
    11221 acgcccgcgg ccacgaacgc gaccgccggc acgatcggtg gcagcggtcc accagcctcg
    11281 tgcagcatgc gcagcagcgc gacgttctcg acgacatcga gccccgctgc gacgagcggc
    11341 gcccatgacg cgtaccaggc ggcgcgcgcg gcgccgagcg cccgggcgga gcggccgcgc
    11401 cggcccaggg cgcgggccgc gagcgcgcac agcgccgaga tcgggatcca gtacagggcc
    11461 aggaaggcga agtccaggcg ggtgtgcgcg accgcgtccg ccaggcgcga ggcgtcccag
    11521 tcgcgcacga gctcgcgcag gccctcgggc gtgccggcga actccagccc gatcaccccg
    11581 cagtgcggca ggtcgcgcag cgcgcatggc tcgatgacgg ggaagcgcag accgacggcg
    11641 acggttgcgg cggtgccgac cacgacgagg gcgagctgcc accagaccgg tccgcgcacc
    11701 aggggcggcg ccgccgaggg ctgacgcagc atgccgatcg cccacgtcag ccccaccacg
    11761 acggcgacgg tccagaacgt gctctccggc ccggcggcgg cgtgctgctg tcgcgacagc
    11821 aggaacagca gcgtcacgag gccgccggcg agcagcacca cgaacggcca gaccctcgcc
    11881 cgcagcgccg acagcagcac ggccgccgcc gcgagcgtcg ccccgacggt gccgaccagc
    11941 ggcggcgcct cctccgccag cgcggagacc gcgagcagcg cgccgccggc ggccagcgcc
    12001 agcgacacca gcagcagccc gaacccggag ccgcgcgtgg cgcccgccac gagcacgtgc
    12061 agcaccagca gcacggcacg gatcggccgg gtgacggcac gcagcggcca cagcccggag
    12121 cggcgcccct ccacggcgga cgcgccgacc agcgcggcgc ggctgacggt gccggcgaac
    12181 aagtcgctgc ccgcgtcgtc catcacggtc tccgcgccga cgcgggactc gcggaacagc
    12241 gcgaagagct gctcggcggc cagcccctcc cgaccgccca cgccccgcag ggcctcctcg
    12301 taccgcgcgc ggaacgcgac gctctcgctg tcgcgcgcgg cgcccgcacg ctcgtcctcg
    12361 cgcgcggcga cggccagccg ctccagctcc tcacgcagga cctcggcctg gacgcggcgg
    12421 gcgacctgga gggcgcagac gggcaggctc ggcggcactg gaaggtcgtc gtcatcgagg
    12481 taggacagct cgcgcgcgca gtcctgggcg gccgcgtcga acgccaccgc gaggtggtca
    12541 cggtcccgct cgacgcgctc ccgcacgggc gcgcccgcgt cggccggcgg ccccacggcg
    12601 gcctcgcgca gggcggcgag ggcgtccgcc gacgagtagc gcagttggcg cagacgcgcg
    12661 ggatccagcg ccagttgggc aaggcgcacg gcggcgtcca gccggcccca gatccagtcg
    12721 ttgacccgcc acgacggctt gtagaaggcc gcgaagtggc cggtgcgcgt gccggtgagc
    12781 ttcaggccga tctcggtggg tgcaccgaac gcgttggggg tg
//
LOCUS       DATMKC010000229        10221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_130855, whole genome shotgun sequence.
ACCESSION   DATMKC010000229 DATMKC010000000
VERSION     DATMKC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130855"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VNU01_06650"
     CDS             complement(<1..654)
                     /locus_tag="VNU01_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWH32335.1"
                     /translation="MLGKDSRFVEGYVFGMWQTNCIVLGDRELGTCVVVDPGQDAAQP
                     VAERLRQQDLRCEAILLTHGHLDHLWAVPELAKELDCPVLLHPEDRWLWDDPVGGLMS
                     VPPDVIEQQLGFRWDPPHERLEEIRDGQTLSFAGFPLTVRHTPGHTPGSSVFLLTDTG
                     EDDPLLLSGDLIFAGSVGRTDLPRGSWDQLLASIHQVVLPLPDATVILPGHGPQTTVG
                     "
     gene            complement(749..3112)
                     /locus_tag="VNU01_06655"
     CDS             complement(749..3112)
                     /locus_tag="VNU01_06655"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWH32336.1"
                     /translation="MTGTVPDEAPERPDTAGAADAGPDQEPSSHAPTKPRQSGVKAVL
                     ARLPMFSDLGSPPPEVLHPLIAVIRKHSPRVDVRTVVRAYQVAAAAHGSQTRRSGEPY
                     ITHPVGVAEILAELGMDTPTVVAALLHDTVEDTELTIEDIRSEFGDEAALLVDGVTKL
                     DRIKVQSREEQQAESLRKMFVAMASDFRVLLIKLADRLHNMKTIHHLPRPKQERIAEE
                     TLAIYAPLAHRLGMQNFKWQLEDLSFGTLHPKRFDEIKAMVSERQPERDRYLDRVVGE
                     IEERLRGVKIRAAVTGRPKHYYSIYEKMIVRGKEFEEIYDLVGVRVIVDSVKDCYAAL
                     GTLHAMWRPIPGRFKDYIAMPKFNLYQSLHTTVVGPDGKSIEIQIRTKAMHRTAEYGV
                     AAHWKYKESAKGSAGSGSGSGDGDTQWLSQMLEWQADTKDSGEFMQNVRMDLYADEVF
                     VFTPKGDVVALPRGSTPVDFAYAVHTEVGHRCIGARVNERLVALEYELRNGDTVEVLT
                     SKAPEAGPSRDWLDFVGSSRARSKIKQWFSRERREDAIEKGREELQRALAKHGMGWKR
                     LIAGDEMKAVAAQMNYTELEAMYRAIGDGHISAANVLTHLSGLLDSGEADEPDESVLL
                     SKIDSGPARPSEAVVVENTHDVWVTLARCCTPVPGDEILGFVTRGRGVSVHRTDCPNA
                     EDLRRDPDRIIPVRWDPAAPATFRVTVQVEALDRKHLLRDVTTVLGDLHVNILSAQVA
                     TQRDRIATLRFTFELADIGHLEHILGQVRRIEGVYDAYRVVPRPAQH"
     gene            complement(3294..3833)
                     /locus_tag="VNU01_06660"
     CDS             complement(3294..3833)
                     /locus_tag="VNU01_06660"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWH32337.1"
                     /translation="MKMRIELDTKVLRDCVRDVPDFPKPGVVFKDITPLLADPTAFST
                     AVDAIVVSFGRGTIDKVVGIEARGFIVAAPVAYHFGAGFVPLRKKGKLPFDTLGESYD
                     LEYGTEELEIHADAFEPGERVLIVDDVLATGGTAEAACNLVERAGATVAGLAFVIELE
                     FLEGAKKLSDREYISLLQY"
     gene            complement(3838..4848)
                     /gene="secF"
                     /locus_tag="VNU01_06665"
     CDS             complement(3838..4848)
                     /gene="secF"
                     /locus_tag="VNU01_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWH32338.1"
                     /translation="MSQTITRGPATGGGFKRFFTNQSQFDFVGRSRMWALISLVLIAV
                     SLLALGVRQLNFGIDFTGGTTFIVTGAAGGFGAEDLREAVGATGVDEVSAQVVDGGEG
                     AMVTTPSIEEVGGEQQDRVLDAIAEVAGVERGEIDVSAVGPRWGEQISRQAIRALVVF
                     LVLVALYIAIRFEWRMALAALVTLAHDVIVTVGIYALVGFQVTPASVIALLTILGYSL
                     YDTVVVFDRVTEDTARLSSVSTQTYGEVANSSLNEVLIRSLSTSVTSLLPVGSLLFIG
                     ATLLGAETLSDLALALFVGMAIGTYSSIAVATPALVWLKEREPRYAELKDKIETRRGR
                     KA"
     gene            complement(4845..6356)
                     /gene="secD"
                     /locus_tag="VNU01_06670"
     CDS             complement(4845..6356)
                     /gene="secD"
                     /locus_tag="VNU01_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWH32339.1"
                     /translation="MRKGRLAVGLVASLAVVAAMWGAILGLGLEPQLGLDLRGGVSIT
                     LIPAEGQGEIDEAVLDQTVQVIRQRIDAIGVAEPDIARQGETVMVQLPGVADREQAEE
                     VIGRTAVLQFRRVLEVIPFGAENYPETGESCAAAQDGVPPADEEVVLCERAFGPATGP
                     GEQPEPLPAGQWTKYRLGEVIVSGDDVTDARAAVDPSGISWHVELELDRDGADAFEEV
                     TGQLACEPADSVLRQMAIVLDGVVESAPGVASDVACGQGIGGGEAIITTGGGREDAQE
                     LAVVLRTGALPIELEFAQSSSVSPTLGEASLRAGLLAGLIGLILVAVYMTALYRGMGL
                     AAVAELVVFGLLVYGMVIVLGEWIGFTLTLAGIAGIIVSVGIAADSSIIYRERYRDEI
                     RAGRTVRTAAEHAFSKAWRTNLTGNTVSFLAAAVLYVLAVGPVRGFAFTLGLSTLIDT
                     IVFGTFTRSLFGLIARSPKLARSPWVGLRAETVAPDIVAAATARSASGKGGTR"
     gene            complement(6385..7140)
                     /locus_tag="VNU01_06675"
     CDS             complement(6385..7140)
                     /locus_tag="VNU01_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWH32340.1"
                     /translation="MERVDAHGVPIGGRTPTGEEQTTPEQSTGRSRIPLLDRVQAHER
                     TSTFIAAADEFLGAQGFTEHGFRHANLVGHIAYNVLTHLDYDFRSCELAAVAGYLHDI
                     GNVVSRAHHGQTSAMLAYDLLKELQVEPADMAQVLSAVGNHEEQYGEAVSPISAAVIL
                     ADKSDVHRSRVRKNASIEMDIHDRVNFAVEASFLRVDPAARTITLELQIDTAISQVLE
                     YFEIFLERMVMCRRAARTLDCDFKITVNDVPVL"
     gene            complement(7150..7470)
                     /gene="yajC"
                     /locus_tag="VNU01_06680"
     CDS             complement(7150..7470)
                     /gene="yajC"
                     /locus_tag="VNU01_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWH32341.1"
                     /translation="MIALLAQAETGSGGALSGLLFPVLMIAVFYLLLIRPQQRKAKAQ
                     QALVRSVAVGDRVITIGGVHATVMSVDEDTMRLEIAPGTVITMARTAVARKLVDADAP
                     SDGV"
     gene            complement(7547..8584)
                     /gene="ruvB"
                     /locus_tag="VNU01_06685"
     CDS             complement(7547..8584)
                     /gene="ruvB"
                     /locus_tag="VNU01_06685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWH32342.1"
                     /translation="MEEILTTTELPGDADLDASLRPRSLEDFLGQDRVKEQLWLVLEG
                     ARSRGAVADHLLFSGPPGLGKTSLAGIVAAEMGAQFRMTSGPALERPGDLAAILTGLE
                     PGDVLFVDEIHRLSRQVEEILYPAMEDFRLDIVVGKGPGARAIRLPLPAFTLVGATTR
                     TGLITSPLRDRFGFAARLDYYAAEELARILARSSRILEIPLAGDAAAEIARRSRGTPR
                     IANRLLKRVRDYAEVREDGTMTAAVAQRALEVFEVDARGLDRLDRGVLDALCRNFGGG
                     PVGLSTLAVGVGEETETVEDVVEPFLIQQGLLARTPRGRVATVAAYRHLGLEPPAGAQ
                     TPLFDGGPGPA"
     gene            complement(8592..9176)
                     /gene="ruvA"
                     /locus_tag="VNU01_06690"
     CDS             complement(8592..9176)
                     /gene="ruvA"
                     /locus_tag="VNU01_06690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWH32343.1"
                     /translation="MIAMLRGRVVSARLGELVLDVHGVGYRVRVPPASFGKPGDELVL
                     HTHLAVREDAMTLYGFPEAAARDLFEVLLSVTGFGPKLALAAIGTLGAEGLRRAVLAE
                     DVHAITAVPGVGKKSAQRVILELREKLGAGAGGDLPGGGELGGRAEVREALAALGYAP
                     AEVQRALQSLPDGGDAPVEELLRQALRALDPAAV"
     gene            complement(9173..9685)
                     /gene="ruvC"
                     /locus_tag="VNU01_06695"
     CDS             complement(9173..9685)
                     /gene="ruvC"
                     /locus_tag="VNU01_06695"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWH32344.1"
                     /translation="MRVLGVDPGLSRCGVGVVEGPVARPRVLRAGVIRTPAGDPVATR
                     LAALHAELTALIAAERPDAMAVERVFFNANVRTAMSVGQAAGVALLCAAQAGVPVVEY
                     TPTQVKSSVAGHGGADKAQVTFMVRALLRLAEAPKPADAADALALALCHLQHAPLAAA
                     GGAATPGRNA"
     gene            9931..>10221
                     /locus_tag="VNU01_06700"
     CDS             9931..>10221
                     /locus_tag="VNU01_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32345.1"
                     /translation="MRHPTVALAALAVAVAALAAPAGAETTVVTGSLGTSVSISTAPS
                     AAVDLGSLALGLNTAGGGTIAVTANTAYTVTVAADKAAMTQWNGTAYGTTALG"
BASE COUNT         1413 a         3802 c         3574 g         1432 t
ORIGIN      
        1 gccgaccgtc gtctgcggcc cgtggcccgg caggatcacc gtggcgtcgg gcagcggcag
       61 gaccacctgg tggatcgacg cgaggagctg gtcccagctt ccgcgcggga ggtcggtccg
      121 ccccacggag ccggcgaaga tcaggtcgcc cgacagcagc agcgggtcgt cctcgccggt
      181 gtcggtcagc aggaacaccg acgagcccgg cgtgtggccg ggcgtgtggc gcacggtcag
      241 cgggaagccg gcgaacgaca gcgtctgccc gtcgcggatc tcctccaggc gctcgtgcgg
      301 cgggtcccag cggaagccga gctgctgctc gatgacgtcc gggggcacgc tcatgagccc
      361 gcccaccggg tcgtcccaca gccagcggtc ctccgggtgc agcaggaccg ggcagtccag
      421 ctccttggcc agctccggca cggcccacag gtggtcgagg tggccgtggg tgagcaggat
      481 cgcctcgcag cgcaggtcct gctggcggag gcgctcggcg acgggctggg cggcgtcctg
      541 gccggggtcg accaccacgc aggtgcccag ctcgcggtcg cccagcacga tgcagttggt
      601 ctgccacatg ccgaagacgt agccctcgac gaaccggctg tccttgccga gcatccacca
      661 acctcctgac gccgcgcgcg cggcgaacgg gtgcccccac tgcggtatct cgcggtgccc
      721 gcacccgttc gaacggggaa ggggcaggct agtgctgggc ggggcgcggc accacccggt
      781 aggcgtcgta cacaccctcg atgcggcgca cctggcccag gatgtgctcg aggtggccga
      841 tgtcggccag ctcgaacgtg aagcgcagcg tcgcgatgcg gtcgcgctgc gtggccacct
      901 gcgccgacag gatgttcacg tgcaggtcgc cgagcaccgt ggtcacgtcg cgcagcaggt
      961 gcttgcggtc cagcgcctcc acctgcacgg tcacgcggaa cgtcgccggc gccgccgggt
     1021 cccagcgcac cgggatgatg cggtcggggt cgcggcgcag gtcctcggcg ttggggcagt
     1081 cggtgcggtg caccgacacg ccgcggccgc gcgtgacgaa acccaggatc tcgtcgcccg
     1141 gcacgggcgt gcagcagcgc gccagcgtca cccagacgtc gtgggtgttc tcgaccacga
     1201 ccgcctcgga cggccgcgcc ggcccgctgt cgatcttgct gagcaggacg ctctcgtcgg
     1261 gctcgtcggc ctcgccgctg tccagcaggc cggacaggtg cgtgagcacg ttggcggcgg
     1321 agatgtggcc gtcgccgatg gcgcggtaca tcgcctccag ctcggtgtag ttcatctgcg
     1381 cggcgacggc cttcatctcg tcgcccgcga tcaggcgctt ccagcccatg ccgtgcttgg
     1441 ccagcgcgcg ctgcagctcc tcgcggccct tctcgatcgc gtcctcgcgc cgctcccgcg
     1501 agaaccactg cttgatcttg gagcgcgcgc gcgaggagcc gacgaagtcc agccagtcgc
     1561 ggctcgggcc ggcctccggc gccttggagg tcagcacctc cacggtgtcg ccgttgcgca
     1621 gctcgtactc cagcgccacc aggcgctcgt tcacgcgcgc gccgatgcag cggtggccca
     1681 cctcggtgtg cacggcgtag gcgaagtcca ccggcgtgga gccgcgcggc agcgcgacca
     1741 cgtcgccctt cggcgtgaac acgaacacct cgtcggcgta caggtccatg cgcacgttct
     1801 gcatgaactc gccggagtcc ttggtgtcgg cctgccactc cagcatctgc gacagccact
     1861 gcgtgtcgcc gtcgccggag ccgctgcccg agcccgcgga gcccttggcg gactccttgt
     1921 acttccagtg cgccgcgacg ccgtactcgg cggtgcggtg catcgccttg gtgcggatct
     1981 ggatctcgat ggacttgccg tccgggccca ccaccgtggt gtgcagcgac tggtagaggt
     2041 tgaacttcgg catcgcgatg tagtccttga agcgccccgg gatggggcgc cacatcgcgt
     2101 gcagggtgcc cagcgccgcg tagcagtcct tcacggagtc cacgatgacc cggacgccga
     2161 ccaggtcgta gatctcctcg aactccttgc cgcgcacgat catcttctcg tagatcgagt
     2221 agtagtgctt ggggcggccg gtcaccgcgg cgcggatctt cacgccgcgc aggcgctcct
     2281 cgatctcgcc gaccacgcgg tccaggtagc ggtcccgctc cggctggcgc tcggagacca
     2341 tcgccttgat ctcgtcgaag cgcttggggt gcagcgtgcc gaaggacagg tcctccagct
     2401 gccacttgaa gttctgcatg cccaggcggt gggccagcgg cgcgtagatc gccagggtct
     2461 cctcggcgat gcgctcctgc ttggggcgcg gcaggtggtg gatcgtcttc atgttgtgca
     2521 ggcggtcggc cagcttgatc agcaggacgc ggaagtccga cgccatggcc acgaacatct
     2581 tgcgcaggct ctccgcctgc tgctcctcgc ggctctgcac cttgatgcgg tccagcttgg
     2641 tgacgccgtc caccagcagc gccgcctcgt cgccgaactc cgagcggatg tcctcgatgg
     2701 tgagctcggt gtcctccacc gtgtcgtgca gcagcgccgc gaccacggtg ggcgtgtcca
     2761 tgcccagctc ggccaggatc tcggcgacgc cgaccgggtg ggtgatgtag ggctcgccgc
     2821 tgcggcgcgt ctgcgagccg tgcgcggcgg cggcgacctg gtaggcgcgc accaccgtgc
     2881 gcacgtccac gcgcggcgag tgcttgcgga tgaccgcgat gagcgggtgg aggacctccg
     2941 gcggcggcga gccgaggtcg gagaacatcg gcaggcgggc cagcaccgcc ttcacaccgg
     3001 actggcgagg cttcgtgggc gcgtgcgagg aaggctcctg gtccgggccg gcgtccgccg
     3061 ctccggccgt gtccggccgc tccggcgcct cgtccgggac ggtgcccgtc acctgaggcg
     3121 cggtggcctg ctgggtccgt tcctcagcgc tcgtgacggc cctccggtcg cggtcgttgg
     3181 ttgggttctc caccataccg ccggatccgc cgccgaagat gcgcaaaggg ttgaaccgct
     3241 cgtccgaaat accggacgaa cgttccaggt tgggaaggcg ggggcgcggg gggtcagtac
     3301 tgcaggaggc tgatgtactc gcggtcggag agcttcttgg ccccctccag gaactcaagc
     3361 tcgatcacga acgccaggcc cgccacggtc gcgccggcgc gctccaccag gttgcacgcc
     3421 gcctcggcgg tgccgcccgt ggccagcacg tcgtccacga tcagcacgcg ctcaccgggc
     3481 tcgaacgcgt cggcgtggat ctccagctcc tcggtgccgt actccaggtc gtagctctcc
     3541 cccagcgtgt cgaacggcag cttgcccttc ttgcgcagcg gcacgaagcc ggccccgaag
     3601 tggtacgcca ccggcgcggc gacgatgaag ccgcgcgcct cgatgcccac caccttgtcg
     3661 atggtgccgc ggccgaagct caccacgatc gcgtccacgg ccgtggagaa cgcggtcgga
     3721 tcggccagca gcggcgtgat gtccttgaag acgacgccgg gcttggggaa gtccggcacg
     3781 tcgcggacac agtcgcgcag caccttggtg tccagctcga tgcgcatctt catgcttcta
     3841 ggccttccgt ccgcggcgtg tctcgatctt gtccttcagc tccgcgtagc gcggctcgcg
     3901 ctccttcagc cacaccagcg ccggggtggc caccgcgatc gaggagtacg tgccgatcgc
     3961 catgcccacg aacagcgcca gcgccaggtc gctcagcgtc tccgcgccca gcagcgtcgc
     4021 gccgatgaac agcagcgagc ccactggcaa cagcgacgtg accgacgtcg acagcgagcg
     4081 gatcagcacc tcgttcagcg aggagttcgc cacctcgccg taggtctgcg tggacaccga
     4141 gctcagccgc gcggtgtcct ccgtgacgcg gtcgaacacc accaccgtgt cgtacagcga
     4201 gtagcccagg atcgtcagca gggcgatcac cgacgccggc gtcacctgga agccgaccag
     4261 cgcgtagatg cccacggtca cgatgacgtc gtgcgccagg gtgaccagcg ccgccagcgc
     4321 catgcgccac tcgaagcgga tggcgatgta cagcgccacc agcaccagga acaccaccag
     4381 ggcgcggatc gcctggcggc tgatctgctc gccccagcgc gggccgaccg cgctcacgtc
     4441 gatctcgccg cgctccacgc ccgccacctc ggcgatcgcg tcgagcacgc ggtcctgctg
     4501 ctcgccgccg acctcctcga tggacggcgt ggtcaccatc gcgccctcgc cgccgtcgac
     4561 gacctgcgcg ctgacctcgt ccacgcccgt ggcgcccacg gcctcgcgca ggtcctcggc
     4621 gccgaagccg ccggcggcgc ccgtgacgat gaacgtcgtg ccgccggtga agtcgatgcc
     4681 gaagttgagc tggcgcacgc ccagcgccag caggctcacg gcgatcagca ccaggctgat
     4741 cagcgcccac atgcgcgagc ggccgacgaa gtcgaactgc gactggttgg tgaagaagcg
     4801 cttgaacccg ccgccggtgg cgggcccgcg cgtgatggtc tggctcatcg ggtgcccccc
     4861 ttgccggaag cggaccgggc ggtggccgcg gccacgatgt cgggcgccac ggtctcggcc
     4921 cgcaagccga cccagggcga gcgcgccagc ttcggcgagc gcgcgatgag gccgaacagg
     4981 ctgcgcgtga acgtgccgaa cacgatcgtg tcgatcagcg tcgacagtcc cagcgtgaac
     5041 gcgaagccgc gcaccgggcc gaccgccagc acgtacagga ccgcggcggc caggaacgac
     5101 accgtgttgc cggtgaggtt ggtacgccac gccttggaga acgcgtgctc ggcggcggtg
     5161 cgcaccgtgc gcccggcgcg gatctcgtcg cggtagcgct cgcggtagat gatcgagctg
     5221 tccgccgcga tgccgacgga cacgatgatg ccggcgatgc cggccagggt gagcgtgaag
     5281 ccgatccact cgcccagcac gatcaccatg ccgtacacca gcaggccgaa caccaccagc
     5341 tcggcgaccg ccgccaggcc catgccgcgg tacagcgccg tcatgtagac cgccaccagg
     5401 atcaggccga tgagcccggc caagaggccc gcgcgcagcg acgcctcgcc cagggtgggc
     5461 gacacgctgg aggactgcgc gaactccagc tcgatcggca gggcgccggt gcgcaggacc
     5521 acggccagct cctgggcgtc ctcgcgcccg ccgccggtgg tgatgatcgc ctcgccgccg
     5581 ccgatgccct ggccgcacgc gacgtcgctg gccacgccgg gcgcggactc caccacgccg
     5641 tccagcacga tcgccatctg gcgcagcacc gagtccgccg gctcgcaggc gagctgcccg
     5701 gtgacctcct cgaacgcgtc ggcgccgtcg cggtccagct ccagctccac gtgccagctg
     5761 atcccggagg ggtccacggc ggcgcgcgcg tccgtgacat cgtcgccgct gacgatcacc
     5821 tcgcccagcc ggtacttggt ccactgcccg gcgggcagcg gctcaggctg ctcgcccggc
     5881 ccggtggccg ggccgaacgc gcgctcgcac agcacgacct cctcgtcggc cggcggcacg
     5941 ccgtcctgtg ccgccgcgca ggactcgccc gtctcggggt agttctccgc cccgaacggg
     6001 atgacctcca ggacccggcg gaactgcagc acggccgtgc ggccgatgac ctcctccgcc
     6061 tgctcgcggt cggcgacccc cggcagctgc accatcaccg tctcgccctg gcgggcgatg
     6121 tcgggctcgg cgacgccgat ggcgtcgatg cgctggcgga tcacctggac cgtctggtcc
     6181 aggaccgcct cgtcgatctc gccctgcccc tccgccggga tcagcgtgat gctcacgccg
     6241 ccccgcaggt ccaggccgag ctgtggctcc agccccaggc ccaggatcgc gccccacatg
     6301 gccgcgacca cggccaggga cgcgaccagg ccaacggcca gtcgtccttt gcgcatgtcg
     6361 agtcctgctt ccccaccgac cccgctacag gaccggtacg tcgttgacgg tgatcttgaa
     6421 gtcgcagtcc agcgtccgcg ccgcgcggcg gcacatgacc atgcgctcca agaagatctc
     6481 gaagtattcc agcacctggc tgatcgcggt gtcgatctgc aactccagcg tgatggtgcg
     6541 cgccgccggg tccacccgca ggaacgacgc ctcgaccgcg aagttcacgc ggtcgtggat
     6601 gtccatctcg atggaggcgt tcttgcgcac gcggctgcgg tgcacgtcgg acttgtccgc
     6661 caggatcacc gcggccgaga tcggggagac cgcctcgccg tactgctcct cgtggttgcc
     6721 cacggcggac agcacctgcg ccatgtcggc cggctccacc tggagctcct tgagcaggtc
     6781 gtaggccagc atcgcggagg tctggccgtg gtgcgcgcgg ctgaccacgt tgccgatgtc
     6841 gtgcaggtag ccggccacgg ccgccagctc gcaggagcgg aagtcgtagt ccaggtgcgt
     6901 gagcacgttg taggcgatgt ggccgaccag gttggcgtgg cggaagccgt gctcggtgaa
     6961 gccctgcgcc cccaggaact cgtccgccgc ggcgatgaac gtcgacgtgc gctcgtgggc
     7021 ctggacgcgg tccagcagcg ggatgcggct gcgccccgtg gactgctccg gggtggtctg
     7081 ctcctcgccg gtgggcgtgc gccccccgat gggcacgccg tgggcgtcca cgcgctccat
     7141 gacggctccc tagaccccgt cgctgggcgc gtcggcgtcg accagcttcc gggccacggc
     7201 cgtgcgcgcc atcgtgatga cggtgccggg cgcgatctcc aggcgcatcg tgtcctcgtc
     7261 gaccgacatc accgtggcgt gcacgccgcc gatggtgatc acgcggtcgc ccacggcgac
     7321 ggagcgcacc agcgcctgct gcgccttggc cttgcgctgc tgcgggcgga tcagcagcag
     7381 gtagaacacg gcgatcatca ggacggggaa cagcagcccg ctgagcgctc ctccggagcc
     7441 ggtctcggcc tgggcaagca gtgcgatcat gacagggatt cctcgcgtcg ggattgggcc
     7501 ttcggccgac ggccgacgat agcagaatgg ccctgccggc cccggatcat gccgggccgg
     7561 gtcccccgtc gaacagcggc gtctgcgcgc cggccggcgg ctccaggccc aggtggcggt
     7621 aggcggccac ggtggcgacg cggccgcgcg gggtgcgcgc cagcagcccc tgctggatca
     7681 ggaacggctc caccacgtcc tcgaccgtct cggtctcctc ccccacgccg accgccaggg
     7741 tggacagccc caccgggccg ccgccgaagt tgcggcacag cgcgtccagc acgccgcggt
     7801 ccaggcggtc cagcccgcgc gcgtccacct cgaacacctc cagcgcccgc tgggccaccg
     7861 ccgcggtcat ggtcccgtcc tcgcgcacct ccgcgtagtc gcgcacgcgc ttgagcaggc
     7921 ggttggcgat gcgcggcgtg ccgcggctgc gccgggcgat ctccgcggcg gcgtcgccgg
     7981 ccagcgggat ctccaggatg cgggagctgc gcgccaggat gcgcgcgagc tcctcggcgg
     8041 cgtagtagtc caggcgcgcg gcgaagccga agcggtcgcg cagcggcgag gtgatcaggc
     8101 ccgtgcgggt ggtggcgccc accagcgtga acgccggcag cggcaggcgg atcgcgcggg
     8161 cgcccggccc cttgccgacc acgatgtcca ggcggaagtc ctccatcgcc gggtacagga
     8221 tctcctccac ctggcgggac aggcggtgga tctcgtccac gaacagcacg tcgccgggct
     8281 ccaggccggt gaggatcgcg gccaggtcgc cgggccgctc cagcgccggc ccgctggtca
     8341 tgcggaactg cgcccccatc tcggcggcga cgatgccggc caggctggtc ttgcccaggc
     8401 cgggcggccc cgagaacagc aggtggtcgg ccacggcgcc gcggctgcgg gcgccctcca
     8461 gcaccagcca gagctgctcc ttgacgcggt cctggcccag gaagtcctcc aggctgcgcg
     8521 ggcgcagcga cgcgtccagg tcggcgtcgc ccggcagctc ggtggtggtg aggatctctt
     8581 ccatgtcagt cctagacggc cgcggggtcc agcgcgcgca gcgcctggcg cagcagctcc
     8641 tcgaccggcg cgtcgccgcc gtccggcagc gactgcagcg cccgctgcac ctcggccgga
     8701 gcgtagccaa gcgcggccag cgcctcgcgg acctccgccc ggccgcccag ctccccgccc
     8761 cccggcaggt cgccgccggc gcccgcgccg agcttctcgc gcagctccag gatcacgcgc
     8821 tgcgcgcttt tcttgcccac gccgggcacg gccgtgatcg cgtgcacgtc ctcggccagc
     8881 acggcgcggc gcaagccctc ggcgcccagc gtgccgatgg ccgccagcgc cagcttcggg
     8941 ccgaagccgg tgacgctcag cagcacctcg aacaggtcgc gggcggcggc ctccgggaag
     9001 ccgtacagcg tcatggcgtc ctcgcgcacg gcgaggtggg tgtgcaggac cagctcgtcg
     9061 ccgggcttgc cgaacgacgc gggcggcacg cggacgcggt agcccacgcc gtgcacgtcc
     9121 aggaccagct caccgaggcg tgcggacacc acccgcccgc gcagcatcgc gatcatgcgt
     9181 tcctccctgg tgtggccgcg ccgccggccg cggccaacgg cgcgtgctgc agatggcaca
     9241 gcgccagggc cagggcgtcg gcggcgtcgg cgggcttggg cgcctccgcc aggcgcagca
     9301 gcgcccgcac catgaacgtg acctgggcct tgtccgcgcc gccgtggccg gccaccgacg
     9361 acttgacctg cgtcggcgtg tactccacca ccggcacgcc cgcctgcgcc gcgcacagca
     9421 gcgccacccc ggcggcctgg ccgacgctca tggcggtgcg cacgttggcg ttgaagaaca
     9481 cccgctccac cgccatcgcg tccgggcgct ccgcggcgat cagcgcggtc agctccgcgt
     9541 gcagcgcggc caggcgcgtg gcgaccgggt cgcccgccgg ggtgcggatc accccggcgc
     9601 gcagcacgcg cggccgggcc accggcccct cgaccacgcc cacgccgcag cgcgacaggc
     9661 cggggtcgac gccgagcacg cgcacggggc ctccttcgcc gtgtcgccgg cgcgaccggc
     9721 ggcgacacat ggttgcactt ggggtcgaac ttgtgttcgg cgcaggctag cagacggcgg
     9781 cgcggcgatg gcgcaaccac ggccgtccgc cgggggctag tcacaccgag tcatttccgg
     9841 attagcacat gcgcagcggt cagtacgccc gctgcaatga ccgaagagag ggtgtacgca
     9901 ccttttggcg tcgcctgttc ggaggacaca atgcgtcacc ccaccgtcgc cctcgccgca
     9961 ctggcagtcg cggtcgccgc acttgccgcg ccggccggtg cggagaccac cgtggtcacc
    10021 ggcagcctcg gcacgtccgt gtccatctcc accgccccca gcgccgcagt ggacctgggc
    10081 agcctggccc tcggcctcaa caccgccggc ggcggcacca tcgccgtcac cgccaacacc
    10141 gcctacacgg tcaccgtggc cgccgacaaa gccgcgatga cccagtggaa cggcaccgcc
    10201 tacggcacca ccgccctggg c
//
LOCUS       DATMKC010000230         6796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_343757, whole genome shotgun sequence.
ACCESSION   DATMKC010000230 DATMKC010000000
VERSION     DATMKC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343757"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VNU01_06705"
     CDS             <1..495
                     /locus_tag="VNU01_06705"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWH32346.1"
                     /translation="IANLMMAQLLHLEGEDPEKDIALYINSPGGSVTSALAILDTMEF
                     IKPDVSTFCMGQAASAAAILLAAGTKGKRYALPHSRILIHQPHGGAEGQSVDIEIQAR
                     EISRIRDLLDDILAEKTGQPKERIGRDTDRDFIMTAAEARDYGLIDEVIASRKTQAAA
                     LKAS"
     gene            690..1961
                     /gene="clpX"
                     /locus_tag="VNU01_06710"
     CDS             690..1961
                     /gene="clpX"
                     /locus_tag="VNU01_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWH32347.1"
                     /translation="MAKFGESGDLLKCSFCAKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEEFAETPELVAGDLPKPAEIFGFLNDYVVGQDDAKKTLAVAVYNHYKRIRVGSTG
                     RDDEVELAKSNILLLGPTGSGKTLLAQTLARLLNVPFAIADATALTEAGYVGEDVENI
                     LLKLIQAADFDVKKAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNVLFICGGAFAGLEHIIEQRIGRKGVGFGAEVFSKK
                     DTDLSNLHSQTLPEDLIKFGLIPEFVGRLPVVCSVEQLDQAALVQILTEPKNALVKQY
                     RKFFEYDGVNLEFTADALEAIADQAILRGTGARGLRAIMEEVLLNTMYELPSMNDIGE
                     CVIDGDTVREKVNPTLVPRTAAEPIRRERSA"
     gene            complement(2026..2244)
                     /locus_tag="VNU01_06715"
     CDS             complement(2026..2244)
                     /locus_tag="VNU01_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32348.1"
                     /translation="MAEFAERSQAALGTMFGNAIRPHVPDERLDDVVRAFFATVEALL
                     VQGTDPETRRRLVALTLDALTEGRLRPA"
     gene            2310..4949
                     /locus_tag="VNU01_06720"
     CDS             2310..4949
                     /locus_tag="VNU01_06720"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWH32349.1"
                     /translation="MSAHDAAHDSGRELPKAYEPAEVEDRLYQWWETSGFFHADPGGH
                     GEPFSIVIPPPNVTGSLHIGHALDNALQDAVVRRARMRARNAVWIPGTDHAGIATQNV
                     VEKQLAAQGLTRHDLGREAFVERVWQWREESGGTILTQLRRLGASCDWDREAFTFDEQ
                     RSKAVRKVFVDLYEQGLIYRGNRLINWCPRCVSAISDIEVDHSEEEGELAFLRYPGSD
                     GSDGVVVATTRAETMLGDTGIAVHPDDERYAHLVGKTVRLPLMDRDIPVFADSHVDPE
                     FGTGAVKVTPAHDPNDHEMGQRHGLPAIDVMTDEAAINANGGAYEGLDRFEARRKVKE
                     DLDALGLLVKVEAHTHSVGHCSRCGTIVEPRLSDQWFVKVRPLAEQAAKAVRDGRTAF
                     VPERNAKPFLDWLDNLNDWCISRQIWWGHRIPAWYDADGGVHVLREDPTPEEAEARGL
                     TRDPDVLDTWFSSALWPFTTLGWPDDTPELRAWYPTTVLVTGYDINTFWVSRMMLMAL
                     RFMDDVPFHTVLNHGLVRDRFGKKMSKSFGNVIDPLELIDRFGADALRFALFRAAAPG
                     QDVPLAEEWVEGARRFANKLWNAARFVLLQLGDVTPADGLPDTGALEVEDRWILSRLE
                     ACRAEVEAAWEDYDVAAASRALYHFVWDEYCDWYLELAKLRDDRAGKLVLAHVLDVAL
                     RLLHPLMPFITEEIWRTLRRTEDTIMRAEWPPATSVRDADAEAAVEAVQEAVTELRRF
                     RAEHNLAPATRIDVVARASAARRAPLQAGVDGIRRLAGVARWSWADEAGEPAGGVGTV
                     VLTDAELAVPLEGLIDLGEERERLERELAKAEAEVKRAQGKLANEAFTAKAPPQVVEA
                     ERAKAAEWADTAATLRAQLDSLS"
     gene            4946..6262
                     /locus_tag="VNU01_06725"
     CDS             4946..6262
                     /locus_tag="VNU01_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWH32350.1"
                     /translation="MSAGVPSYDDVVRELFARQPNRMVPDLDRIRTLTELLGRPDQAF
                     PAIQVTGTNGKTTVSLMISALLGALGLSAGTYTSPHLQDVRERIRVAGEPISREEMVE
                     GLAYLAPFVLETDDRHPEALTFFEILTALSFTHFGDKPVDVGVYEVGMGGAWDATNLV
                     RGEVAVLNSVSLDHLELGSTLQAIAREKAGVIKDGAVVVSAAQDDDVADVIAAAAEAH
                     DARLVVAGRDFDILERGLAVGGQYLTLRGVTGEVDEIYLPLHGAHQARNAAVALAAVE
                     GFLGFAGGLDAEVIREGFAAVRSPGRVEVVRRRDAAPVVLDGAHNPAGAATLAQTLRT
                     EFGHRHRVFVLGVLGDKDVEGIVEALLPVADHLVVTEPPSGRAAPTDRIAKALHAAGR
                     TFETAADVPAAIELASGVATEEDAVVVTGSLYMVGAARDALGLEPA"
     gene            6274..6678
                     /gene="ndk"
                     /locus_tag="VNU01_06730"
     CDS             6274..6678
                     /gene="ndk"
                     /locus_tag="VNU01_06730"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWH32351.1"
                     /translation="MSTERTLILVKPDGVKRRLVGEVVSRIERKGLTISRMELKTISR
                     ETAEQHYGEHRERPFFGELVDFITSGPLVAMVVEGPDAVKSMRNIMGATNPVEATPGS
                     IRGDFATVIGENIVHGSDSPENGEREIAIFFP"
BASE COUNT         1075 a         2406 c         2430 g          885 t
ORIGIN      
        1 atcgcgaacc tgatgatggc ccagctcctg cacctggaag gcgaggaccc cgagaaggac
       61 atcgccctct acatcaactc cccgggcggg tcggtcacca gcgcgctggc gatcctggac
      121 accatggagt tcatcaagcc ggacgtgtcg acgttctgca tgggccaggc cgcctctgcc
      181 gcggcgatcc tgctggcggc cggcaccaag ggcaagcgct acgccctgcc ccacagccgg
      241 atcctcatcc accagccgca cggcggcgcc gagggccagt cggtggacat cgagatccag
      301 gcccgggaga tcagccggat ccgcgacctg ctcgacgaca tcctcgccga gaagaccggc
      361 cagcccaagg agcgcatcgg ccgcgacacc gaccgcgact tcatcatgac cgccgccgag
      421 gccagggact acggcctcat cgacgaggtc atcgcctcgc gcaagacgca ggcggccgcg
      481 ctgaaggcca gctaggggcc ggcgtccgcc ccggcgagcg gttcgcccgc gaatcgggag
      541 caatgccgtg tccgtgtcac ccggccccgc tacgatgaac ggcggcagcg caccggcggt
      601 ccagttccgc cgggacggtg ccgataggac catcaggccg cagggccctc cggggtcgtg
      661 gcgggccgca agacgggagc gcgaggcgca tggccaagtt cggtgagagc ggcgacctgc
      721 tgaagtgctc gttctgcgcg aagtcgcaga agcaggtcaa gaagctcatc gccggaccgg
      781 gcgtgtacat ctgtgacgag tgcatcgacc tgtgcaacga gatcatcgaa gaggagttcg
      841 ccgagactcc cgagctcgtc gccggcgacc tgccgaagcc cgccgagatc ttcggctttc
      901 tcaacgacta cgtcgtgggc caggacgacg ccaagaagac cctcgcggtc gccgtctaca
      961 accactacaa gcgcatccgc gtcggctcca ccggccgtga cgacgaggtc gagctggcca
     1021 agtccaacat cctcctgctc ggccccaccg gctccggcaa gacgctgctc gcgcagaccc
     1081 tcgcccggct gctgaacgtg ccgttcgcca tcgcggacgc cacggcgctc accgaggccg
     1141 gctacgtggg cgaggacgtc gagaacatcc tgctgaagct gatccaggcc gccgacttcg
     1201 acgtgaagaa ggccgagacc gggatcatct acatcgacga ggtcgacaag atcgcccgca
     1261 agagcgaaaa cccgtcgatc acgcgggacg tctccggcga gggcgtgcag caggcgctgc
     1321 tgaagatcct ggagggcacg gtggccagcg tgccgccgca gggcgggcgc aagcaccccc
     1381 accaggagtt catccagatc gacaccacca acgtgctgtt catctgcggc ggggcgttcg
     1441 ccggcctgga gcacatcatc gagcagcgca tcggccgcaa gggcgtgggc ttcggcgccg
     1501 aggtgttctc caagaaggac accgacctgt ccaacctgca ctcgcagacg ctgccggagg
     1561 acctgatcaa gttcgggctg atcccggagt tcgtcggccg ccttccggtg gtgtgcagcg
     1621 tcgagcagct ggaccaggcg gcgctggtgc agatcctcac cgagcccaag aacgcgctgg
     1681 tcaagcaata ccgcaagttc ttcgagtacg acggcgtgaa cctggagttc accgcggacg
     1741 ccctggaggc catcgccgac caggcgatcc tgcgcggcac cggcgcccgc ggcctgcgcg
     1801 cgatcatgga ggaagtcctc ctgaacacga tgtacgagct gccgtcgatg aacgacatcg
     1861 gcgagtgcgt catcgacggc gacaccgtgc gggagaaggt caacccgacg ctggtgccgc
     1921 gcacggcggc ggagccgatc cggcgcgagc gctccgcctg accttccccg ttcgaacggg
     1981 tgcgggcacc gcgacatccc gcagtggggg cacccgttcc ggtggtcacg ccggccgcaa
     2041 gcgtccctcg gtcagggcgt ccagcgtcag cgccaccagg cggcggcgcg tctcgggatc
     2101 ggtgccctgc accagcaggg cctcgaccgt ggcgaagaac gcgcgcacga catcgtccag
     2161 ccgctcgtcc ggcacgtgcg gcctgatggc attgccgaac atcgtgccga gcgccgcctg
     2221 agaccgttcg gcgaactccg ccacgaccgc tgagcagtgg gaggccagcc ggggcagagg
     2281 cagagcgggg cggccgtaga ctggcccgca tgtctgcaca cgacgctgcg cacgactccg
     2341 ggcgcgagct gccgaaggcg tacgagccgg ccgaggtcga ggaccggctc taccagtggt
     2401 gggagacctc cgggttcttc cacgccgacc ctggcggcca cggcgagccg ttctccatcg
     2461 tcatcccgcc gcccaacgtc accggctccc tgcacatcgg ccacgcgctg gacaacgcgc
     2521 tgcaggacgc cgtcgtccgc cgcgcgcgca tgcgcgcgcg caacgcggtg tggatccccg
     2581 gcaccgacca cgcgggcatc gccacgcaga acgtggtgga gaagcagctc gccgcgcaag
     2641 ggctgacgcg ccacgacctg ggccgcgagg cgttcgtgga gcgcgtgtgg cagtggcggg
     2701 aggagtccgg cggcaccatc ctgacgcagc ttcgccgcct gggcgcgtcc tgcgactggg
     2761 accgcgaggc gttcaccttc gacgagcagc gcagcaaggc cgtgcgcaag gtgttcgtgg
     2821 acctgtacga gcagggcctg atctaccggg gcaaccgcct catcaactgg tgcccgcgct
     2881 gcgtgtcggc gatcagcgac atcgaggtcg accacagcga ggaggagggc gagctcgcgt
     2941 tcctgcgcta ccccggctcg gacggttcgg acggcgtggt ggtcgccacc acgcgcgcgg
     3001 agaccatgct cggcgacacg ggcatcgcgg tgcaccccga cgacgagcgc tacgcgcacc
     3061 tggtcggcaa gaccgtgcgg ctgccgctga tggaccgcga catccccgtg ttcgccgaca
     3121 gccacgtcga cccggagttc ggcaccggcg ccgtgaaggt caccccggcg cacgacccca
     3181 acgaccacga gatgggccag cgccacggcc tgcccgcgat cgacgtgatg accgacgagg
     3241 cggcgatcaa cgccaacggc ggggcatacg agggcctgga ccgcttcgag gcgcgccgca
     3301 aggtcaagga ggacctggac gcgctgggcc tgctggtcaa ggtcgaggcg cacacgcact
     3361 cggtggggca ctgctcgcgc tgcgggacga tcgtggagcc gcgcctgtcc gaccagtggt
     3421 tcgtgaaggt ccgccccctg gccgagcagg ccgccaaggc cgtgcgcgac ggccgcaccg
     3481 ccttcgtccc ggagcgcaac gccaagccgt tcctggactg gctggacaac ttgaacgact
     3541 ggtgcatcag ccggcagatc tggtggggcc atcgcatccc cgcctggtac gacgccgacg
     3601 gcggcgtgca cgtgctgcgc gaggacccca caccggaaga ggccgaggcg cgcgggctca
     3661 cccgcgaccc ggacgtgctg gacacctggt tcagctccgc gctgtggccg ttcaccacgc
     3721 tgggctggcc cgacgacacg ccggagctgc gcgcctggta cccgaccacc gtgctggtga
     3781 ccggctacga catcaacacg ttctgggtca gccgcatgat gctgatggcg ctgcgcttca
     3841 tggacgacgt gccgttccac accgtgctga accacggcct ggtgcgcgac cgcttcggca
     3901 agaagatgtc gaagtcgttc ggcaacgtca tcgacccgct ggagctgatc gacaggttcg
     3961 gcgccgacgc gctgcgcttc gcgctgttcc gtgccgccgc ccccggccag gacgtgcccc
     4021 tggccgagga gtgggtcgag ggcgcgcggc gcttcgcgaa caagctctgg aacgccgcgc
     4081 gcttcgtgct gctgcagctg ggcgacgtga cgccggcgga cggcctgccg gacacgggcg
     4141 cgctcgaggt ggaggaccgc tggatcctgt cgcggctgga ggcctgccgg gccgaggtcg
     4201 aggccgcgtg ggaggactac gacgtcgccg ccgcgtcccg ggcgctgtac cacttcgtgt
     4261 gggacgagta ctgcgactgg tacctggagc tggccaagct gcgcgacgac cgcgccggca
     4321 agctggtgct cgcccacgtg ctcgacgtgg cgctgcgcct gctgcacccg ctgatgccgt
     4381 tcatcaccga ggagatctgg cgcacgctgc ggcgcaccga ggacacgatc atgcgcgccg
     4441 agtggccgcc ggccaccagc gtccgcgacg ccgacgccga ggcggcggtc gaggcggtgc
     4501 aggaggccgt caccgagctg cgccgcttcc gcgcagagca caacctggcg ccggccacgc
     4561 gcatcgacgt ggtcgcgcgc gcgagcgcgg cgcgccgcgc gccgctgcag gccggcgtcg
     4621 acggcatccg ccgcctggcc ggcgtggcgc gctggagctg ggcggacgag gccggcgagc
     4681 cggccggcgg cgtcggcacg gtggtgctca ccgacgccga gctggccgtg ccgctggagg
     4741 ggctgatcga cctgggcgag gagcgcgagc gcctggagcg cgagctcgcc aaggcggagg
     4801 ccgaggtcaa gcgcgcccag ggcaagctcg ccaacgaggc cttcacggcc aaggccccgc
     4861 cgcaggtcgt cgaggcggag cgggcgaagg cggccgagtg ggccgacacg gccgccaccc
     4921 tgcgcgcgca gctggacagc ctgtcatgag cgccggcgtg ccctcctacg acgacgtcgt
     4981 gcgggagctg ttcgcccgcc agcccaaccg catggtcccc gacctggacc gcatccgcac
     5041 gctgacggag ctgctcggcc ggccggacca ggccttcccg gccatccagg tcaccggcac
     5101 caacggcaag accaccgtct cgctcatgat cagcgcgctc ctgggcgcgc tcggcctgtc
     5161 cgcggggacc tacacctcgc cgcacctgca ggacgtgcgc gagcgcatcc gcgtggccgg
     5221 cgagccgatc tcccgcgagg agatggtgga ggggctggcg taccttgcgc cgttcgtgct
     5281 ggagaccgac gaccgccacc ccgaggccct gacgttcttc gagatcctca ccgcgctgtc
     5341 gttcacgcac ttcggggaca agcccgtgga cgtcggcgtc tacgaggtcg gcatgggcgg
     5401 cgcgtgggac gccaccaacc tcgtgcgcgg cgaggtggcg gtgctgaact cggtcagcct
     5461 cgaccacctc gaactcggat ccaccctgca ggccatcgcg cgggagaagg ccggggtgat
     5521 caaggacggc gccgtcgtgg tgtccgccgc gcaggacgac gacgtggcgg acgtgatcgc
     5581 ggcggccgcc gaggcgcacg acgcccgtct ggtggtggcc ggccgcgact tcgacatcct
     5641 ggagcggggg ctggccgtgg gcggccagta cctgaccctg cgcggcgtga ccggggaggt
     5701 cgacgagatc tacctgccgc tgcacggcgc acaccaggcg cgcaacgccg cggtggcgct
     5761 ggcggcggtg gagggtttcc tgggcttcgc cggcggtctg gacgccgagg tcatccgcga
     5821 gggcttcgcc gccgtgcgct cgcccggccg cgtggaggtg gtgcgccggc gggacgccgc
     5881 gcccgtcgtg ctcgacggcg cgcacaaccc ggcgggcgcc gccaccctgg cgcagacgct
     5941 gcgcaccgag ttcgggcacc gccaccgcgt gttcgtgctc ggcgtgctgg gcgacaagga
     6001 cgtcgagggc atcgtcgagg cgctgttgcc ggtggcggac cacctggtgg tgacggagcc
     6061 gccgagcggc cgcgccgccc cgacggatcg catcgccaag gcgctgcacg ccgccgggcg
     6121 gacgttcgag accgcggcgg acgtgcccgc cgcgatcgag ctggcctccg gcgtggccac
     6181 cgaggaggac gccgtggtgg tgaccggctc gctgtacatg gtcggcgccg cccgagatgc
     6241 gctcgggctg gagcccgcat agcatccgcg cccatgagca cagaacgcac cctgatcctg
     6301 gtcaagcccg acggcgtgaa gcggcgtctg gtcggcgagg tggtctcccg catcgagcgc
     6361 aagggactga ccatcagccg catggaactg aagaccatca gccgcgagac ggccgagcag
     6421 cactacggcg agcaccgcga acgcccgttc ttcggcgagc tggtcgactt catcacctcc
     6481 gggccgctgg tggcgatggt cgtcgaaggc cccgacgcgg tgaagagcat gcgcaacatc
     6541 atgggcgcca ccaacccggt cgaggcgacg ccgggcagca tccggggcga cttcgccacc
     6601 gtgatcggcg agaacatcgt gcacgggtcg gactcgccgg agaacggcga gcgcgagatc
     6661 gcgatcttct ttccctgacg gcgcgcgccg gctgggggca ctgtccgttc gggaaggtcg
     6721 gctgcgcgta atgcgtagtg accaggggta accgggggtg cacgcgttcc cccaccccca
     6781 caggagtgcc ccccga
//
LOCUS       DATMKC010000231         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_29827, whole genome shotgun sequence.
ACCESSION   DATMKC010000231 DATMKC010000000
VERSION     DATMKC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29827"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..607)
                     /locus_tag="VNU01_06735"
     CDS             complement(86..607)
                     /locus_tag="VNU01_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH32352.1"
                     /translation="MKDAIMTSAYPSIRIADVAGTAGVSSQTVHTHFASKEALFLAAI
                     GELGQELLEMRGGTPPGDVVAIARGLVAEYERYGDVNWALLPLERDSTAVAAALQAGR
                     AGHRAWLEASFAPLLPVHPRRRRQALDALYAATDVGTWKLLRRDLGLSSARTRAVIET
                     LVRGALTACDDSH"
     gene            complement(691..1122)
                     /locus_tag="VNU01_06740"
     CDS             complement(691..1122)
                     /locus_tag="VNU01_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32353.1"
                     /translation="MSRKPLLAAALLSVATTVVTTAVSLRHPDLPDEPLGLRFPGDVR
                     VHLALGLGSGVAAPWPMPLIALVAALKDGPGRVGPARTCAAIGATMLVGTVAEPASWG
                     LRPRSDAARLTVVLHLLSGSALFLAATLRRPEAGVDSRVQP"
     gene            1255..1920
                     /locus_tag="VNU01_06745"
     CDS             1255..1920
                     /locus_tag="VNU01_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWH32354.1"
                     /translation="MSDVGGNYVQMNELVHAGLRRDLDRIGQVARQPLTAERREALAR
                     RVSWLVEFLHHHHASEEEAIWPLAVRKRPQLGGLMEAMEAEHGALAAAADGLHEAAAA
                     YAGDGSESKRQALVAALDRMRITCLPHLEHEEAAAVPQLVQTLDDREWAKVDKRFRRG
                     LGPKDLGWIAMWLLDDLDPAHARFLRGQLPRPVLTLLTWRWGRPYAREASLAWGELAG
                     VRG"
     gene            complement(1967..>2162)
                     /locus_tag="VNU01_06750"
     CDS             complement(1967..>2162)
                     /locus_tag="VNU01_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32355.1"
                     /translation="ADFVSGQIVGVLLGTAFRFWAFRRYVFPMQDVRRRAGDAVASVP
                     GNVEAAAAEPPPHGPERDAF"
BASE COUNT          299 a          796 c          799 g          268 t
ORIGIN      
        1 cccgcccgcc ggtgttcacg atcttcctgc cacccgcgcg cgccgacgac gtggtgggcg
       61 cgccgcgccc ggccggcggc cctcgctagt gggagtcgtc gcaggccgtc agagccccac
      121 ggaccagcgt ctcgatgacg gcgcgggtac gggctgagga gagccccagg tcacggcgga
      181 gcagtttcca ggtgccgaca tcggtggccg cgtacagggc gtccaaggct tgccggcggc
      241 gcctggggtg gacgggcagc agcggggcga agctcgcctc gagccacgcc cggtgcccgg
      301 cgcggcccgc ctgcagggcg gcggcgacgg cggtgctgtc ccgctccagc ggcagcagcg
      361 cccagttgac gtcgccgtac ctctcgtact ccgcgaccag gccgcgcgcg atcgccacga
      421 cgtcgccggg aggcgtgcca ccgcgcatct ccagcagctc ctggccgagc tcgccgatgg
      481 cggcgaggaa cagcgcctcc ttcgacgcga aatgcgtgtg caccgtctgc gacgacactc
      541 cggccgtgcc ggcgacgtcc gcgatccgga tggacgggta cgccgaggtc atgatcgcgt
      601 ccttcatcgc ctgcacgatc ttccttcggg tctgatcccg cttctcggcg cgggcgacca
      661 tccggtaccg gcggcgcgac ggctgcgcgg tcacggctgc acacgactgt ccacacccgc
      721 ttcggggcga cgcagggtgg ccgccaggaa cagcgccgag ccgctcagca ggtgcaacac
      781 gaccgtgagc cgtgctgcgt ccgagcgcgg gcgcagcccc cacgacgcgg gctccgccac
      841 cgtcccgacc agcatcgtcg cgccgatggc cgcgcacgtg cgtgctggcc cgacccggcc
      901 cgggccgtcc ttgagcgccg cgaccagggc gatcagcggc atcggccacg gcgccgcgac
      961 tcccgatccc aggcccagcg ccaggtgcac gcgaacgtca ccgggaaagc gcagcccgag
     1021 cggctcgtcg ggcaggtccg ggtgtcgtag cgacaccgcc gtcgtgacca cggtggtggc
     1081 gaccgacagc agcgcggcgg ccagcagcgg cttccggctc atccgttccc ccttgacccc
     1141 tcgcgggcac cgggctgccc ggccaccagg gtgatattga cttgtccccg agtcaagaca
     1201 attatgtctg gcggccgccg ttccagccgg ccgggaagca cccagggtgg cgaggtgagc
     1261 gacgtgggcg gcaactacgt gcagatgaac gagctggtcc atgccgggct gcggcgcgac
     1321 ctggaccgca tcggccaggt ggcccggcag ccgttgaccg ccgagcggcg cgaggccctg
     1381 gcccggcgcg tcagctggct ggtggagttc ctccatcacc accacgcgag cgaggaggag
     1441 gcgatctggc cgctggccgt gcgcaagcgg ccacagctgg gcgggctgat ggaggcgatg
     1501 gaagccgagc acggcgcctt ggcggccgcg gcggacggcc tgcacgaggc ggccgcggcc
     1561 tacgccggcg acgggtcgga gagcaagcgg caggcactgg tggccgcgct cgatcgcatg
     1621 cggatcacct gcctgcccca cctcgagcat gaggaagccg cggccgtgcc gcagctggta
     1681 cagaccctgg acgaccggga gtgggcgaag gtcgacaagc ggttccgccg agggctgggc
     1741 ccgaaggatc tcggctggat cgcgatgtgg ctgctcgacg acctcgaccc ggcccacgcc
     1801 cggttcctcc gcggccagct tccccgcccc gtcctgacgt tgctgacctg gcgctggggc
     1861 cgcccctacg ctcgcgaggc gtccctcgcc tggggtgagc tcgccggcgt gcgcgggtag
     1921 cggcgccgtc gccggacccg acgacggcaa cgccgggccg gcgaggctag aacgcgtcgc
     1981 gttccggacc gtgcggaggc ggttccgccg cggccgcttc gacgttcccg gggaccgagg
     2041 cgactgcgtc gccggcgcgc cggcgcacgt cctgcatcgg gaacacgtag cgacggaacg
     2101 cccagaaccg gaacgccgtg ccgagcagga cgccgacgat ctggccgctg acgaagtcgg
     2161 cg
//
LOCUS       DATMKC010000232         7970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_63398, whole genome shotgun sequence.
ACCESSION   DATMKC010000232 DATMKC010000000
VERSION     DATMKC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63398"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..842
                     /locus_tag="VNU01_06755"
     CDS             195..842
                     /locus_tag="VNU01_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32356.1"
                     /translation="MRGVWIPGEPGLGPWLDDLRRIAPMPAPAVRLEFTLRPGRWVDL
                     YTLVERAVRTTGAGRALHGLIATKRWGAPAGMRLRPVSAVTVARARPPGDTLLDVSAD
                     ARPRGKADKAALRDLIAEAWGARPVLPGESWADVRTSAAGSLLGGVEAILDRLEPVLG
                     RDPRGRAWQEFFPFDDRIVWLRLLRDPAGPALRLRAGAERGTGAERDAASPERPS"
     gene            complement(839..2836)
                     /locus_tag="VNU01_06760"
     CDS             complement(839..2836)
                     /locus_tag="VNU01_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWH32357.1"
                     /translation="MDATPVSAAKMRHYLDGRWGWAREQTREFLRQPLFQPVHGLAVE
                     DYRERVYAQLQALASGPWVRVGFPPEYGGRSDVGAFLTAFETLAHGDLSLLVKAGVHW
                     GLFGGAVLHLGTTRHHEAYLRKVISAELPGCFAMTELGHGSDVQAIRTTATYDPAAGE
                     FVIHTPEESARKEYIGNAARHGRMAAVFAQLCTLGQCHGVHAFLVPIRGADGSPAPGV
                     RIGDSGHKMGLNGVDNGRLWFDQVRVPRESLLDRYGQVAPDGTYTSPIADETKRFFTM
                     LGTLVQGRVSIAGAGVSAAKSALTIAVRYGDERRQFSPPGSDREIVLLDYTAHQRRLL
                     PLLARTYALHFAQEALVARFHEVFTSLDADGERRERPRRELESLAAGLKALSTSHATR
                     TVQECREACGGAGYLSENRLGELKADTDVFTTFEGDNTVLLQLVAKGLLTGYRDEFGS
                     LDMLGMVRFVADQAVETVLERTGARGLVQTIADVVNRDDDGDLRDPAWHLELYRWREE
                     HVLGGLARRLRKGIDSGCDAFGVFNECQDHVLLAARAHVERVVVECFHGAVGRCGDPE
                     LRAVLASLSELHALAGIEAERGWFLEHGRISNARAKGVIAAVTQLCGHLRPHARALVD
                     AFGIPDEAIAAPIALGGAVPAAPVIAAAPAESPVPGRALTS"
     gene            3050..4687
                     /locus_tag="VNU01_06765"
     CDS             3050..4687
                     /locus_tag="VNU01_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWH32358.1"
                     /translation="MNVHRRTVAWLAAVAMLAAVAGVAPLGAAEHTKTDTVVTSHDGT
                     PIAVTVFKPATAGADTPVPMIMHSHGWGGSRTTAISAFQAELDEGFGVLSFDQRGFGQ
                     TPGPANVQDPELEGRDVIALIDFIAGLDWVAKDVKPGVPANAAETAKGNTGHVGVRED
                     AEVDPSDPVLFAMGLSYGGGYQFIGALTETRETGRTRFNALAPEITWFDLADSLAPAK
                     VARTAWISLLYATGLQAHRQDIHAATAFGMATGQWPDGTYPGIANIHDRFRANSPVAF
                     VEDGKHLDVPVLIGQGSSDNLFNLNQGVRNFEITLTDAARAKSLFVGYNGGHALPNVL
                     PPGYPADVQVGGNADPCSQPAGGFGKLRRDFFRAVIAGENPRALLGAGQAYHVATADG
                     VCVHTDSVAANTELVAAAAPGLPSAAVTTTGAGAPQHLHLAAGPLRVAGVPVLEADVT
                     TVGADQRVFFGLSVGETPATARVVHNNLLPLRELLPIVREGRVLELAGMAVDVPAGQH
                     LYLTVSPLSDMYFGHGSTRTPGAVTLENIRVRVPVQP"
     gene            complement(4762..5451)
                     /locus_tag="VNU01_06770"
     CDS             complement(4762..5451)
                     /locus_tag="VNU01_06770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWH32359.1"
                     /translation="MALILLVRHGQASFGADDYDVLSDVGERQARLVGERLRVLPRVD
                     VVLHGAMTRQQRTAELLVEGLGADVRRERDERWDEYDHEALLSGAMPTDADREAFAAE
                     MAAAEDPRRAFQRAFERATARWTAGAHDHDYHEPFPAFLERVHAAMADLAAGLGRDDC
                     AVVATSGGVIAAVCARLLGLDGPAWANLNRVIVNTSVTKLVHGRSGLTLLSVNDHAHL
                     EGAHRDLLTYR"
     gene            complement(5442..6536)
                     /locus_tag="VNU01_06775"
     CDS             complement(5442..6536)
                     /locus_tag="VNU01_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWH32360.1"
                     /translation="MTAVDDAAERDGARPVRDEDAFDVGAAHAWLAARVPELAGPPPQ
                     VRQFSGGSSNLTYLLRYPGRDLILRRPPAGRKAASAHDMGREYRVQSLLAPVYPYVPA
                     MVAFCDDASVIGAEFYVMERVAGLIPRASLPPEVPGDPDTVRRLCEAVVDRLVELHQV
                     DAEAAGLGELGRGRGYVERQVRGWSERYRAARTWNVPSFERVMAWLEDNRPEDVAHCV
                     IHNDYRFDNVVLDPADPARVVGVLDWELATLGDPLMDLGSSLAYWVQSDDDRLFKAFR
                     RQPTHLPGMLTRQEVVARYAERTGLAVGDWRFYEVFGLFRVAVIAQQIYLRYHRRETR
                     NPAFRHYWLAVRLLHRRCRKVMRKGPIGWR"
     gene            complement(6533..7762)
                     /locus_tag="VNU01_06780"
     CDS             complement(6533..7762)
                     /locus_tag="VNU01_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32361.1"
                     /translation="MDFALSPRAQPLLERVQGFIRDEIDPYELERRREMEFGADPWAV
                     SPWVEELKAKAKAQGLWNLFLPDPDQGAGLSNVEYAPLAEAMGRSSVAAEVFNCNAPD
                     SGNMELLHAYGSPAQKERWLRPLLEGEIRSAFCMTEPDVASSDATNMEATAVVDGGEI
                     VLNGRKWWSTGVGHPRCEVLIFMGLTDPAAHKYSQHSMVLVPRDTPGVTVGRMLRTMG
                     FLDAPGGHGEVTFDNVRLPLDHVIGGPGRAFEIAQNRLGPGRIHHCMRLIGLAERALE
                     LACERAAGRVAFGKPLANLGGNRERIADARIAIQQARLLVLHAAWLLDTQGLLGALSA
                     VSQIKVAVPNMAQRVVDMAMQIHGGAGLSDDEPFGAMWTTARALRIADGPDEVHRGVV
                     ARIELGPYLQRARDAGA"
BASE COUNT         1042 a         2979 c         2896 g         1053 t
ORIGIN      
        1 ctgtgtcgcc aggccaccac cggtggtcgg cagcggtggt ggcctggcga cacagggcct
       61 agccctgctg ggtgtggccg gtggcgccgc ggcgatgatc cgccgccgcc tctcgtagcc
      121 gacgcgacat cgacagggcc ggacctacgg gtccggccct gtcgttttcc cgcccaaagg
      181 gcaaacgggc gaacatgcgc ggtgtgtgga tccccggtga gccaggcctc ggcccgtggc
      241 tggacgacct gcggcgcatc gcgccgatgc ccgcgcccgc ggtgcggctg gagttcacct
      301 tgcggccggg ccgctgggtc gacctctaca cgctggtgga gcgcgccgtg cgcacgaccg
      361 gagccgggcg ggcgctgcac gggctcatcg ccacgaagcg ctggggcgca ccggccggga
      421 tgcggctgcg gccggtgtcg gcggtcaccg tcgcccgcgc ccggccgccc ggcgacacac
      481 tgctggacgt gtcggcggac gcgcgaccgc gcggcaaggc cgacaaggcg gccctccgcg
      541 acctcatcgc ggaggcttgg ggcgcccgcc cggtcctgcc aggcgagtcc tgggccgacg
      601 tgcgcaccag cgccgcgggg tcgctgctcg gcggggtcga ggccatcctg gacagactgg
      661 aaccggtgct cggccgcgac ccgcgcgggc gcgcgtggca ggagttcttc ccctttgacg
      721 accgcatcgt ctggctccgg ctgctgcgcg accccgccgg gccggcgctt cgcctgcgcg
      781 cgggcgccga acggggcacg ggcgccgaac gggacgcggc ctcgccggag cggccctcct
      841 agctggtgag cgcccggccc gggaccggcg actcggcggg cgcggccgcg atgacgggcg
      901 cggccggcac cgcaccgccc agggcgatgg gggccgcgat tgcctcgtcc gggatgccga
      961 atgcgtccac gagcgcgcgt gcgtgcgggc gcagatgccc gcacagttgc gtgacggcgg
     1021 cgatcacgcc cttggcgcgg gcgttggaga tgcggccgtg ctccaggaac cagccgcgct
     1081 ccgcctcgat gcccgccagg gcgtgcagct cgctcagcga cgccagcacc gcgcgtagct
     1141 ccgggtcgcc gcagcgcccg accgccccgt ggaagcactc caccaccacg cgctcgacgt
     1201 gcgcccgcgc cgccagcagc acgtggtcct ggcactcgtt gaagacgccg aaggcgtcgc
     1261 agccgctgtc gatgcccttg cgcaggcggc gggcgaggcc gccgagcacg tgttcctcgc
     1321 gccagcggta cagctccaag tgccaggcgg ggtcgcgcag gtcgccgtcg tcgtcgcggt
     1381 tgaccacgtc ggcgatggtc tgcaccaggc cgcgcgcgcc ggtgcgctcc agcaccgtct
     1441 ccaccgcctg gtccgccacg aagcgcacca tgccgagcat gtccaggctg ccgaactcgt
     1501 cgcggtagcc ggtcagcagc cccttggcca ccagttgcag caggaccgtg ttgtcgccct
     1561 cgaaggtcgt gaacacgtcc gtgtcggcct tgagctcgcc caggcggttc tccgacaggt
     1621 agccggcgcc gccgcacgcc tcgcggcact cctggaccgt gcgcgtggcg tgcgaggtcg
     1681 acagcgcctt caggcctgct gccagcgact ccagctcccg gcgcgggcgc tcccggcgct
     1741 ccccgtcggc gtccagggac gtgaacacct cgtggaagcg ggcgaccagg gcctcctggg
     1801 cgaagtgcag ggcgtacgtg cgcgccagca ggggcagcag gcgccgctgg tgggccgtgt
     1861 agtccagcag cacgatctcg cggtcgctgc ccggcgggct gaactggcgg cgctcgtcgc
     1921 cgtagcgcac cgcaatggtc agcgcgctct tcgcggcgct gacgcccgcg ccggcgatgc
     1981 tcacgcggcc ctggaccagc gtgcccagca tggtgaagaa gcgcttggtc tcgtcggcga
     2041 tcgggctggt gtaggtgccg tccggcgcga cctggccgta gcggtccagc aggctctccc
     2101 gcggcacgcg cacctggtcg aaccacagcc ggccgttgtc gacgccgttg aggcccatct
     2161 tgtggccgga gtcgccgatg cggacgccgg gcgccgggct cccgtccgcg ccgcggatcg
     2221 gcaccaggaa cgcgtgcacg ccgtggcact gccccagcgt gcacagctgc gcgaacaccg
     2281 ccgccatgcg gccgtgccgc gccgcgttgc cgatgtactc cttgcgcgcg gactcctccg
     2341 gcgtgtggat gacgaactcc cccgcggcgg ggtcgtacgt cgccgtggtg cggatggcct
     2401 ggacgtccga gccgtggccg agctcggtca tcgcgaagca gcccggcagc tcggcgctga
     2461 tgaccttccg caggtaggcc tcgtggtggc gcgtggtgcc caggtgcagc acggcgccgc
     2521 cgaacagccc ccagtgcacg ccggccttca ccagcagcga caggtcgccg tgcgcgagcg
     2581 tctcgaacgc cgtgaggaac gccccgacgt cgctgcgccc gccgtactcg ggcgggaagc
     2641 cgacgcgcac ccacggtccg gaggccagcg cctggagctg ggcgtacacg cgctcgcggt
     2701 agtcctccac ggccaggccg tgcaccggct ggaacagcgg ctggcgcagg aactcgcgcg
     2761 tctgctcgcg cgcccagccc cagcgcccgt ccaggtagtg ccgcatcttg gcggcgctga
     2821 caggtgtggc gtccacggtc gctccttgcg ggcaggacaa acggcgacgt gccccagcgt
     2881 cctaccccgt ccaggcagtt ttcgctcgcg cctgccggac ctgtccggct ttcgcaccgt
     2941 ttctgcatga aggacatcgc ggtgacggcg gcgaaagcaa aggggggaaa gtccaggggg
     3001 atgcatgggc cttcgaggcc cgccgacgag acgccgagga gtccgacccg tgaacgttca
     3061 ccgacgcacc gtggcctggc tggccgccgt cgccatgctc gccgccgtgg cgggcgtcgc
     3121 gccgctgggc gccgccgagc acaccaagac cgacaccgtg gtgaccagcc acgacggcac
     3181 gcccatcgcc gtgacggtgt tcaagccggc cacggccggc gcggacacgc ccgtgccgat
     3241 gatcatgcac tcgcacgggt ggggcggctc ccggacgacg gccatctcgg cgttccaggc
     3301 cgagctcgac gagggcttcg gcgtcctgag cttcgaccag cgcggcttcg gccagacgcc
     3361 cggccccgcc aacgtgcagg acccggagct ggagggccgc gacgtcatcg ccctcatcga
     3421 cttcatcgcc ggcctggact gggtggccaa ggacgtcaag ccgggggtgc cggccaacgc
     3481 cgcggagacg gccaagggca acaccggcca cgtcggggtc cgcgaggacg ccgaggtgga
     3541 cccctcggac ccggtgctgt tcgccatggg cctgtcctac ggcggcggct accagttcat
     3601 cggcgccctc acggagacgc gcgagaccgg gcgcacccgc ttcaacgcgc tggcgccgga
     3661 gatcacctgg ttcgacctgg ccgactccct ggcgcccgcg aaggtggcgc gcaccgcgtg
     3721 gatctcgctg ctgtacgcga cggggctgca ggcgcaccgc caggacatcc acgcggcgac
     3781 cgcgttcggc atggcgaccg ggcagtggcc ggacggcacc taccccggca tcgccaacat
     3841 ccacgaccgc ttccgcgcca actcgccggt ggcgttcgtg gaggacggca agcacctgga
     3901 cgtgcccgtg ctgatcggcc agggctccag cgacaacctg ttcaacctca accagggtgt
     3961 gcgcaacttc gagatcaccc tcaccgacgc cgcgcgcgcc aagagcctgt tcgtgggcta
     4021 caacggcggg cacgcgctgc ccaacgtgct gccgcccggc tacccggccg acgtgcaggt
     4081 cggcggcaac gcggacccgt gctcgcagcc tgcgggcggg ttcggcaagc tgcgccgcga
     4141 cttcttccgc gccgtgatcg cgggcgagaa cccgcgcgcg ctgctcggcg ccggccaggc
     4201 ctatcacgtc gccaccgccg acggcgtctg cgtgcacacc gactcggtgg ccgcgaacac
     4261 cgagctggtg gccgcggccg cgccgggcct gcccagcgcc gcggtgacga ccacgggcgc
     4321 cggcgctccc cagcacctgc acctggcggc cgggccgctg cgcgtggcgg gcgtgccggt
     4381 cctggaggcc gatgtcacca cggtgggcgc ggaccagcgc gtgttcttcg gcctgtcggt
     4441 cggcgagacg ccggcgacgg cgcgggtggt gcacaacaac ctgctgccgc tgcgcgagct
     4501 gctgccgatc gtgcgggagg ggcgcgtcct ggagctcgcc ggcatggccg tggacgtgcc
     4561 cgccgggcag cacctgtact tgacggtgtc gccgctgtcg gacatgtact tcggccacgg
     4621 cagcacccgc acgccgggcg ccgtgaccct ggagaacatc cgcgtgcgcg tgcccgtcca
     4681 gccctagagc cgccgttgcg gagtcgattc tggccactat cggccggaat cggctccgca
     4741 cgcgggcggg gcgggggcgg ttcagcggta cgtgagcagg tcgcggtgcg cgccctcgag
     4801 gtgggcatgg tcgttcacgc tcagcagcgt caggccgctg cgcccgtgca cgagcttggt
     4861 caccgaggtg ttcacgatca cccggttgag gttcgcccac gccggcccgt ccaggcccag
     4921 cagcctcgcg cacacggcgg cgatcacgcc gccggaggtc gccacgactg cgcagtcgtc
     4981 gcggccgagg cccgccgcca ggtcggccat ggcggcgtgc acgcgctcca ggaacgccgg
     5041 gaacggctcg tggtagtcgt ggtcgtgcgc gcccgccgtc cagcgcgcgg tggcgcgctc
     5101 gaacgcccgc tggaacgctc gccgggggtc ctcggcggcc gccatctccg ccgcgaacgc
     5161 ctcccggtcc gcgtcggtcg gcatggcgcc cgacagcagc gcctcgtggt cgtactcgtc
     5221 ccagcgctcg tcgcgctctc ggcgcacgtc ggcgccgagc ccctccacga gcagctccgc
     5281 ggtgcgctgc tggcgcgtca tggcgccgtg cagcacgacg tcgacgcgcg gcaggacgcg
     5341 cagccgctca cccaccaggc gggcctggcg ctcgcccacg tcggacagca cgtcatagtc
     5401 gtccgcgccg aaggacgcct gcccgtggcg caccagcagg atcagcgcca tccgatgggc
     5461 cccttgcgca tgaccttgcg gcagcggcgg tgcaggaggc gcaccgccag ccagtagtgg
     5521 cggaacgccg ggttgcgcgt ctcgcggcgg tggtagcgca ggtagatctg ctgggcgatc
     5581 accgcgacgc ggaacaggcc gaacacctcg tagaagcgcc agtcccccac cgccaggccg
     5641 gtgcgctccg cgtagcgcgc caccacctcc tggcgcgtga gcatgccggg caggtgcgtg
     5701 ggctggcggc ggaacgcctt gaacagccgg tcgtcgtcgg actgcaccca gtacgccagc
     5761 gagctgccga ggtccatgag cgggtcgccg agcgtggcca gctcccagtc caggaccccg
     5821 acgacgcggg cggggtcggc cgggtccagc acgacgttgt cgaaacggta gtcgttgtgg
     5881 atcacgcagt gggcgacatc ctccggccgg ttgtcctcca gccacgccat gacgcgctcg
     5941 aagctgggca cgttccaggt cctggccgcc cggtagcgct ccgaccagcc gcgcacctgt
     6001 cgctccacgt agccccggcc gcggccgagc tcccccagac ccgccgcctc ggcgtcgacc
     6061 tggtgcaact ccaccagccg gtccaccacc gcctcgcaca ggcgccgcac ggtgtccggg
     6121 tcgccgggca cctccggcgg cagcgacgcc cgcgggatca gcccggcgac gcgctccatg
     6181 acgtagaact ccgcgccgat gacggaggcg tcgtcgcaga aggcgaccat ggccggcacg
     6241 tacgggtaca cgggcgcgag cagcgactgc acgcggtact cgcgtcccat gtcgtgcgcg
     6301 gaggcggcct tccggcccgc cggcggccgc cgcaggatca ggtcgcggcc ggggtagcgc
     6361 agcaggtacg tgaggttgga ggacccgccg gagaactgcc gcacctgcgg cggcggcccg
     6421 gcgagctccg gcacgcgggc cgccagccag gcgtgcgcgg cccccacgtc gaaggcgtcc
     6481 tcgtcgcgga cggggcgagc gccgtcgcgc tcggcggcgt cgtcgaccgc cgtcacgcgc
     6541 ccgcgtccct ggcgcgctgc aggtagggcc ccagctcgat gcgcgcgacc acgccgcggt
     6601 gcacctcgtc cgggccgtcg gcgatgcgca gcgcccgggc ggtggtccac atggcgccga
     6661 acggctcgtc gtcggacagg cccgcgccgc cgtggatctg catcgccatg tcgacgacgc
     6721 gctgcgccat gttcggcacc gcgaccttga tctggctcac cgccgacagc gcgcccagca
     6781 gcccctgggt gtccagcagc cacgcggcgt gcaggaccag caggcgcgcc tgctggatcg
     6841 cgatgcgcgc gtcggcgatg cgctcccggt tgccgcccag gttggccagc ggcttgccga
     6901 aggcgacgcg gcccgccgcc cgctcgcagg cgagctccag ggcgcgctcc gccagcccga
     6961 tcaggcgcat gcagtggtgg atgcgcccgg ggcccaggcg gttctgcgcg atctcgaacg
     7021 cgcggcccgg cccgccgatg acgtggtcca gcggcaggcg cacgttgtcg aaggtgacct
     7081 cgccgtggcc gccgggggcg tccaggaagc ccatggtgcg cagcatgcgg ccgaccgtca
     7141 ccccgggcgt gtcgcgaggc acgagcacca tcgagtgctg ggagtacttg tgcgcggcgg
     7201 ggtcggtcag ccccatgaag atcagcacct cgcagcgggg gtggcccacg ccggtgctcc
     7261 accacttgcg gccgttgagc acgatttcgc cgccatcgac caccgcggtg gcctccatgt
     7321 tggttgcgtc ggaggacgcg acgtcgggct ccgtcatgca gaacgccgag cggatctccc
     7381 cctccagcag cggccgcagc cagcgctcct tctgcgcggg gctgccgtag gcgtgcagca
     7441 gctccatgtt gccgctgtcc ggcgcgttgc agttgaagac ctcggccgcc acggacgagc
     7501 ggcccatcgc ctccgccagc ggcgcgtact cgacgttgga caggcccgca ccctggtccg
     7561 ggtcgggcag gaacaggttc cacaggccct gcgccttggc cttggccttg agctcctcca
     7621 cccacgggga cacggcccac gggtcggcgc cgaactccat ctcgcgccgg cgctcgagct
     7681 cgtaggggtc gatctcgtcg cggatgaagc cctggacgcg ttccaggagc ggttgcgcac
     7741 gcggcgacag cgcgaagtcc atctgggcgg ctcctttggc ggtggacgcc acagcatagg
     7801 gaggcggtgt cggtcatcag cgtggctccc ggacaggctc ggcgttcacg gccggtgatc
     7861 ctcgcatgcg cgaagcgccg gcaccgcctc cctatgctgt ggcgtccacc gccaaaggag
     7921 ccgcccagat ggacttcgcg ctgtcgccgc gtgcgcaacc gctcctggaa
//
LOCUS       DATMKC010000233         3179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_356691, whole genome shotgun sequence.
ACCESSION   DATMKC010000233 DATMKC010000000
VERSION     DATMKC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356691"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VNU01_06785"
     CDS             complement(<1..489)
                     /locus_tag="VNU01_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWH32362.1"
                     /translation="MASSRGRAGKLRGWRKIAGATWGVPNDPQIYGDLEVDATGLLRF
                     VDAARAAGVKLTITHLAGKAIAHAYAESPDLNVRLFRGRLIPRDSVDIFFIVSTGGGG
                     ELSGVKVSDADRKPVVEIAGELARRAGSIHAGEDQDLGKTKAMLDKMPLWLLKIMVRV
                     AAW"
     gene            563..1963
                     /gene="proS"
                     /locus_tag="VNU01_06790"
     CDS             563..1963
                     /gene="proS"
                     /locus_tag="VNU01_06790"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWH32363.1"
                     /translation="MANPKRVLTPQSEDFPRWYQDVVAKAEMAENGPARGTMIIKPWG
                     YAIWERIQAEMDARIKATGHVNAYFPMFIPTSFLQREAEHVEGFSPELAVVTHAGGEE
                     LAEPLVVRPTSETVIGDAMSRWIQGYRDLPLLLNQWANVVRWELRPRLFLRTTEFLWQ
                     EGHTAHAAWDEAQEETLKILFQVYEDVIRNVLAVPVVLGRKTDREKFAGAEVTYTLEG
                     LMRDGKALQMGTSHNMGTNFAKAFGIQYTDAEGKVQFCNTTSWGVSTRVVGGLVMSHG
                     DDDGLRLPPEVAPVQVVVLAVKEETIGDAERLAAELNGAGIRAKVDDRINQSFGRRAV
                     EWELKGAPVRIEVGPRDLANGEVTVVRRDEDDKHTVALDLVATQVPELLAHIQESLLE
                     DALRRRDEFTRDVDKGDEIDGAGFFRIPWDALGEDGEAALAERGYSVRCLVKEDGSVP
                     APHDDRNLVAYCAKAY"
     gene            complement(1960..2751)
                     /locus_tag="VNU01_06795"
     CDS             complement(1960..2751)
                     /locus_tag="VNU01_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HWH32364.1"
                     /translation="MRAAHVDPFAGVSGDMLLGAVIDSGVPAEEIVATLDGLGLDGWD
                     LETSTVSRAGLGATQAVLSVRDDGVIRTWAHVREMISGAKVPEPVRFRALATFRRLAE
                     AESAIHRRTIERVHFHEVGALAALLEIVGVCAGLHLLELATIVCGPVAQGVGMRRTEH
                     GLLPIPAPIVLELLRGAPTYSSGVTHELCSPTGAALLAEWADRWGPMPPMAIDRVGYG
                     AGPRGDIDRPHLLRMVIGEAIDTPPAAWERQERQMAEGDQPLDSA"
     gene            2854..>3179
                     /locus_tag="VNU01_06800"
     CDS             2854..>3179
                     /locus_tag="VNU01_06800"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HWH32365.1"
                     /translation="MEFTESTVGALLLAALGVGVLAVLLSVIAITGQRRVREAYRVFS
                     MGSRDDVLTLLQRHIEEVRGLRRDVAGVRQYGDDLRDLLRSTMSRVGTVRYDAFDDMG
                     GRLSFST"
BASE COUNT          459 a         1120 c         1170 g          430 t
ORIGIN      
        1 ccaggccgcg acccgcacca tgatcttcag cagccacagg ggcatcttgt ccagcatggc
       61 cttggtcttg ccgaggtcct ggtcctcccc ggcgtggatg ctgcccgcgc ggcgcgccaa
      121 ctccccggcg atctcgacca cgggcttgcg gtccgcgtcg gagaccttga ccccgctcag
      181 ctccccgccg ccgccggtgg acacgatgaa gaagatgtcc acgctgtcgc gggggatcag
      241 ccggccgcgg aacagccgga cgttgaggtc cgggctctcc gcgtaggcgt gggcgatggc
      301 cttgccggcc aggtgggtga tggtgagctt gaccccggcg gcgcgcgcgg cgtcgacgaa
      361 ccgcagcagg ccggtggcgt cgacctcgag gtcgccgtag atctgcgggt cgttgggcac
      421 gccccaggtg gcgccggcga tcttgcgcca cccgcgcagc ttgccggcgc gtccccgact
      481 gctggccatc cgtcccgcct cctcacccgc ccgttccacc gaccggagcg tacggcggcc
      541 agggccgaat atcctgcact ccatggccaa ccccaagcgc gtgctcactc cgcagtccga
      601 ggacttcccc cgctggtacc aggacgtcgt ggccaaggcc gagatggccg agaacggccc
      661 ggcgcgcggc accatgatca tcaagccgtg gggctatgcg atctgggagc gcatccaggc
      721 ggagatggac gcccgcatca aggcgaccgg gcacgtcaac gcctacttcc cgatgttcat
      781 ccccaccagc ttcctccagc gcgaggccga gcacgtggag gggttctcgc cggagctggc
      841 ggtggtcacc cacgccggcg gcgaggagct ggcggagccg ctggtggtcc ggcccacgtc
      901 cgagacggtg atcggcgacg ccatgtcgcg ctggatccag ggctaccgcg acctgccgct
      961 gctgctcaac cagtgggcga acgtggtgcg ctgggagctg cggccgcggc tgttcctgcg
     1021 caccaccgag ttcctgtggc aggaggggca cacggcgcac gccgcctggg acgaggcgca
     1081 ggaggagacg ctcaagatcc tgttccaggt ctacgaggac gtgatccgca acgtcctggc
     1141 ggtgcccgtg gtgctgggcc gcaagaccga ccgcgagaag ttcgccggcg cggaggtgac
     1201 ctacacgctg gaggggctga tgcgcgacgg caaggcgctg cagatgggca ccagccacaa
     1261 catgggcacg aacttcgcca aggcgttcgg catccaatac accgacgccg aaggcaaggt
     1321 gcagttctgc aacaccacgt cgtggggcgt gtcgacccgc gtggtgggcg gcctggtcat
     1381 gagccacggc gacgacgacg ggctgcggct gccgccggag gtggcgccgg tgcaggtggt
     1441 ggtgctggcg gtgaaggagg agaccatcgg cgacgccgag cgcctggccg ccgagctgaa
     1501 cggcgcgggc atccgcgcca aggtggacga ccgcatcaac cagtcgttcg ggcgccgggc
     1561 cgtggagtgg gagctcaagg gcgcaccggt gcgcatcgag gtgggcccgc gggacctggc
     1621 caacggcgag gtgaccgtgg tgcgccgtga cgaggacgac aagcacaccg tggcgctgga
     1681 cctggtggcc acgcaggtgc ccgaactgct cgcccacatc caggagtcgc tgctggagga
     1741 cgcgctgcgg cggcgcgacg agttcacgcg cgacgtcgac aagggcgacg agatcgacgg
     1801 cgccggcttc ttccgcatcc cgtgggacgc gctgggcgag gacggcgagg cggccctggc
     1861 ggagcgcggc tactccgtgc gctgcctggt caaggaagac gggtccgtgc ccgctcccca
     1921 cgacgatcgc aacctcgtcg cctactgcgc caaggcgtac taggcgctgt ccagcggctg
     1981 atcgccctcg gccatctggc gctcctggcg ttcccacgcc gcgggcgggg tgtcgatcgc
     2041 ctcgccgatc accatgcgca acaggtgcgg ccggtcgatg tcgccgcggg gcccggcgcc
     2101 gtagcccacc cggtcgatgg ccatgggcgg catcggaccc cagcggtcgg cccactcggc
     2161 cagcagcgcg gcgcccgtgg gcgagcacag ctcgtgggtg acgccgctgg agtaggtcgg
     2221 cgcgccgcgc agcagctcca gcacgatcgg cgcggggatc ggcagcaggc cgtgctcggt
     2281 gcggcgcatg ccgacaccct gcgcgaccgg cccgcacacg atggtggcca gctccagcag
     2341 gtgcaggccc gcgcacacgc ccacgatctc cagcagcgcg gccagcgcgc cgacctcgtg
     2401 gaagtgcacc cgctcgatgg tgcggcgatg gatcgcggac tccgcctcgg ccaggcgccg
     2461 gaacgtcgcc agcgcccgga accgcaccgg ctcgggcacc ttggcgccgc tgatcatctc
     2521 gcgcacgtgc gcccacgtcc ggatgacgcc gtcgtcgcgc acgctcagca ccgcctgcgt
     2581 ggcgcccagg ccggcgcgcg acacggtcga ggtctccagg tcccagccgt caaggccgag
     2641 gccgtccagc gtcgcgacga tctcctcggc cggcacgccg ctgtcgatca cggcgcccag
     2701 gagcatgtcg ccgctgacgc cggcgaaggg gtcgacatgg gcggctcgca tgggcgcagc
     2761 gtacgtcggc gggcgcggga aaccgcgcag ttacactcgg gcggcggcct gcgccactcg
     2821 ggcggacggg cggaaactgg tgaggcaagt ctcgtggagt tcacggagtc gaccgtcggg
     2881 gcgctgttgc tcgccgccct cggggtgggc gtgctggcgg tgctgctgtc ggtgatcgcg
     2941 atcaccgggc agcgccgcgt gcgcgaggcc taccgcgtgt tctcgatggg cagccgcgac
     3001 gacgtgctga ccctgctgca gcgccacatc gaggaggtgc gcggcctgcg ccgcgacgtc
     3061 gcgggcgtgc gccagtacgg cgacgacctg cgcgacctgc tgcgctcgac catgtcccga
     3121 gtcggcacgg tgcgctacga cgccttcgac gacatgggcg ggcggctgtc gttctccac
//
LOCUS       DATMKC010000234        15886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_77053, whole genome shotgun sequence.
ACCESSION   DATMKC010000234 DATMKC010000000
VERSION     DATMKC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77053"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VNU01_06805"
     CDS             complement(<1..388)
                     /locus_tag="VNU01_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32366.1"
                     /translation="MSRLLAVLLSCAVLVLPPAGSAGAQEPSPTPSEPGGECVEGDPD
                     GYERVVDLTFPVSGGTRGDFTDTYDAPRSGGRVHQATDVMGRKLQGVHAVLPGEVVVA
                     TGIGEPMPSYGYYLKIRHDNGLESVYV"
     gene            complement(425..667)
                     /locus_tag="VNU01_06810"
     CDS             complement(425..667)
                     /locus_tag="VNU01_06810"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HWH32367.1"
                     /translation="MTSKRLHLTFPEQLVQQPIISHMVKTHGVDVNIRRADVDEHVGW
                     MVLELTGDEAAVNDAQDWLRSQGIEVNDPGGDIVAG"
     gene            complement(743..1006)
                     /locus_tag="VNU01_06815"
     CDS             complement(743..1006)
                     /locus_tag="VNU01_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWH32368.1"
                     /translation="MLGLLLSLLVIGIIAGFLARLIVPGRDPMSFWETVALGVVGSFV
                     GGFLGALLFRGELELGATGILGSIIGAVIALLIYRSVRRPHHA"
     gene            complement(1130..1405)
                     /locus_tag="VNU01_06820"
     CDS             complement(1130..1405)
                     /locus_tag="VNU01_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWH32369.1"
                     /translation="MSVTVRIPTPLRNLTGEQATVDVEGATVGEVVKALDAAHPGIGE
                     RVLGEDGQVRRFVNVFVDDEDIRFQQGLDTPVRDGATVSIIPAVAGG"
     gene            complement(1409..2668)
                     /locus_tag="VNU01_06825"
     CDS             complement(1409..2668)
                     /locus_tag="VNU01_06825"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWH32370.1"
                     /translation="MSYVRGLHCRECGRETAIAPVHVCEFCFGPLEVVYDYDGMRGNV
                     TRESIEQGPRSIWRYADLLPVLPGSDPTERVDLGAGFSPLVPAPRLGAALGLDDLWLK
                     NDTLNPTFSFKDRVVSVALSAARALGFDTAACASTGNLAHSVAAHAAHAGMDAFVFIP
                     ADLEQAKILSTAVYGPNLVAVKGNYDDVNRLCAEVAGEYGWAFVNVNVRPYYAEGSKT
                     IGFEIAEQLGWTLPDHVVAPMASGSMLVKIDKAFSELVKVGLVDDKPVRVSGAQATGC
                     SPISTALKDGSGFVRPVKPDTIAKSLAIGNPADGYYAIDAVRRTGGWMEDVSDTEIVD
                     AIELLARTEGIFAETAGGVTVATLRKLAEQGHVQRGERTVAVISGLGLKTLDVVADRV
                     GPTYTINAGLQDFQSALELGAGQHKES"
     regulatory      complement(2740..2849)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3046..3366
                     /locus_tag="VNU01_06830"
     CDS             3046..3366
                     /locus_tag="VNU01_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32371.1"
                     /translation="MRKLFAIAAAVLMLGAGSALAQGPDANGPAKYGLCTAYFSGQGG
                     EHGNRNDAPPFANLEAAAEEAYEGDEDDPIEDKVRDFCDGTRPSNGRGNGRQDDSSAP
                     NPID"
     gene            3448..4266
                     /locus_tag="VNU01_06835"
     CDS             3448..4266
                     /locus_tag="VNU01_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWH32372.1"
                     /translation="MSTDELGQRLAALPRVRAIYTDLDGTLLGPRGSLLHGPDGRPSA
                     RAAEALVAAAAAGVEVIPVSGRQRKQLENDARLMGLDDCVAEAGVVIVRDGVRHYEWG
                     DLPRGLAGNPHDTMTEAGAVAVLLRAFPDDLRHYEPWHLDREGSHLFHGRVDVAAADR
                     LLDEHGCSWASLIDNGDTGGWPGRSVRAYHLVPRGIDKARAVADDIQARGLRREETLG
                     VGDSLADLAMAASVGTFVIVANGHGEAGDGVVRVDEAMGHGFAAAVEAALSARR"
     gene            complement(4279..5427)
                     /locus_tag="VNU01_06840"
     CDS             complement(4279..5427)
                     /locus_tag="VNU01_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWH32373.1"
                     /translation="MERTILQRANTAVWLAVGSLLLLVGLFWLLREPVGVLLPPILLA
                     MLIVYVLNPLVMWLERRGIARWAGVGIAYLTMALTIGVLVAVLSPLLAEQVRNFANDL
                     PQLGNRLVSGVNDLLARLGANVRVSESLSGDRISEEVERFLSDERNREAVLALLGGIS
                     GVAMGLVHLLVVFIAGPFVAFYVLVDLPRLGRWAHGALPPRHRDEADVLARKLAVVVG
                     GYVRGQLLVATFVGMATSIGLWAIGLRFWLLVGILAGVTNLVPLIGPFVGGLVGVTIA
                     LVTEGPALALLVVLVVTVVQQLDGHVVSPLVVGRNVQLHPLAVLLALLVAGTLYGIFG
                     MLVAIPLVAAVNVVIVHFWRTRVPWLRAERQHHLPAAAESIPSDSGRN"
     gene            complement(5463..6035)
                     /locus_tag="VNU01_06845"
     CDS             complement(5463..6035)
                     /locus_tag="VNU01_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HWH32374.1"
                     /translation="MGRHADPDPRWFWLSLAAAAGKALLAVALVGLAAAGLVRWLGGG
                     DEGEKPVVTLDDTGEPAPADTVTSSPTPTPTGFQDEPAVEGPEATADGGDDGPVRATV
                     QVLNASGDPDKLGDVVEVLEELGYQVVAQSRAARGYDETTVFWSAGFEDEAERLQDTD
                     DRFGVLERNDRLDASIDLHVVIGRDWVADS"
     gene            complement(6039..6320)
                     /locus_tag="VNU01_06850"
     CDS             complement(6039..6320)
                     /locus_tag="VNU01_06850"
                     /inference="COORDINATES: protein motif:HMM:NF023092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HWH32375.1"
                     /translation="MGQPLPPEGGLDARSRALLDFEREWPAAGAETGKEQAIKEAFGF
                     SKARYHQLLAKLIDSAEAYAYDPLTVLRLRRRRGRLDRRRTAEAVRERR"
     gene            6493..7245
                     /locus_tag="VNU01_06855"
     CDS             6493..7245
                     /locus_tag="VNU01_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH32376.1"
                     /translation="MAESLRGKVALVTGGAGGFGAAMGRRLAARGAQVVLADVHEEGA
                     AAVAEEIGGAAVRCDVSSLEDNLAAVAFAVERFGGLDIVALNAGVSTGFGIEDDFDVD
                     KYRRVMGINLDGVVFGLHAALPRLRGRGGGDVIATASLAGLTPVPMDPLYAANKAAVV
                     HLVRSLGPVLGDSGIRVNALCPSFAHTPIITPIREALEGMRTPILPVDAVMDAFERIL
                     DSGQSGQAWFVQYGRPSEPFGFRGVPGPRVEQ"
     gene            complement(7252..8220)
                     /locus_tag="VNU01_06860"
     CDS             complement(7252..8220)
                     /locus_tag="VNU01_06860"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWH32377.1"
                     /translation="MVQHAVITGGAGFLGSHLVDRLLREGWRVTVLDNLVTGRLENLQ
                     AWQGSRLLRFVRQDVTEFLHVPGDVDAVLHFASPASPEDYRALPIQTLKVGSLGTHKA
                     LGLAREKRAVFMLASTSEVYGDPLVHPQPEDYWGNVNPIGERGVYDEAKRFAEAMTLA
                     YHRTHGVPTRIARIFNTYGPRMRPNDGRAIPTFVGQALRGEPITVHGDGGQTRSFGYV
                     DDIVDGLFRLLCSQEDGPVNIGNPDEHTVRELAELVVRMCDSPSEVRLVPRPADDPAQ
                     RRPDITLARTRLGWEPAVPLREGLARTIEWYRSVLGGTGRPATPVS"
     gene            8418..10088
                     /locus_tag="VNU01_06865"
     CDS             8418..10088
                     /locus_tag="VNU01_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWH32378.1"
                     /translation="MRVLGLNAVFHDPAAALVVDGEVLAAAEEERFSRRKHGKPSVPF
                     STWELPEQSAKWCLAQAGLSIGDIDAIAYSYDPALATPRGERVEDLVAGGWDGLRTLY
                     AQRAPLFLRTLFPELEPEQVRFVPHHVAHAASAHLAAPFPTSSVLVLDGRGERWSHLA
                     GRALDGELKVLAQQELPHSLGLLYEELTEHLGFRRSSDEYKVMAMASYGRPRFLGEFR
                     RLVHADGEGGFRVEPIDWSAFAARRSPRTEDFGEDHADLASTVQTRLEEVLLDLARWL
                     HATTGDKVLTMAGGVALNCVANARLAAEGPFEHIWVQPAAGDAGTALGGALHLAQALG
                     DDTLPMTGADLGRGWTDDELEAWLTTAEVPYERPADVAEAVADCLADNGVVAWFQGRS
                     EYGPRALGHRSLLADPRREENLRRLNEVKGREQFRPVAPMVLLERAGEIFTGVIPSPY
                     MLFTHGVRPEWRERIPAVVHVDGTARIQTVDRDSEPLVARMLEGFARRTGVPVVVNTS
                     LNTAGRPMVDDPRDALECFGSGPVDLLALGPFVVRRGAMFAAGPGGGR"
     gene            10085..11629
                     /locus_tag="VNU01_06870"
     CDS             10085..11629
                     /locus_tag="VNU01_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HWH32379.1"
                     /translation="MSAPAPAPSFDVVVPTIGRPSLRALLESLGACDGPRPRRIFLVD
                     DRADQRAPLDVGAPAPWARERLAVLPGRAAGPAAARNAGWRAARAGWVAFLDDDVVVP
                     PEWLARLAADLHGAGPDVGGVQGRITVPLPAGRRPTDWERNVAGLEAAQWATADLAYR
                     RAALESVGGFDERFPRAYREDADLGLRVTESGWRIVRGARRITHPVRPADSRVSVRLQ
                     RGNADDVLMAALHGKGWRERAGVPRGRRRAHLATAGLGALTALAAGRGRRRAAAAAGA
                     GWLVLTAEFAARRIAPGPRDRAEIATMAWTSAAIPFAASWHWLTGWARLPRRLAEPGP
                     IAGAVTSPLPDAVLLDRDGTLIRDVPYNGDPGRVVPMPGAAEALALLRRAGVRVAVVS
                     NQSGVARGLLTREQVDAVHARVEELLGPFDGWFVCPHGPDDGCACRKPAPGLVKEAAA
                     ALGTEPERCALVGDIGSDVDAALAAGARPILVPTPQTLETEIAGAPEVARDLTDAIAR
                     ILRGSR"
     gene            11626..12711
                     /locus_tag="VNU01_06875"
     CDS             11626..12711
                     /locus_tag="VNU01_06875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWH32380.1"
                     /translation="MTATHILAVRLDNDGDVLLAGPAIRALAASGARVTLLCGPRGRR
                     AARILPGVDAVHVFQAPWIDPEPDAVRRDDMDAFVDGVAALRADEALVFTSFHQSPLP
                     TALLLRMAGVARIGAISEDYPGSLLDLRHRVPHDIHEVERGLSLAAAFGYAPPAGDNG
                     RLSVRRTGATPPPFDEPYVVVHPGASVPARAWPPAHHRELVRLLASRGVPVVVTGSPS
                     ERELTREVAGGPSDFVADLGGATTLGELADVLADASAVVVGNTGPAHLAAAVGTPVVS
                     LFAPTVPPIRWRPYKVPQRLLMTDVPCAGCRARECPVAGHPCLSQLTPEMVLGALDSL
                     LAETAAARPQRRSRAARGLGSLVEVGP"
     gene            12708..13664
                     /locus_tag="VNU01_06880"
     CDS             12708..13664
                     /locus_tag="VNU01_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH32381.1"
                     /translation="MRILHWHVHGSWTTSFVHGPHTYLLPVLPDRGPDGRGRARTWDW
                     PASAVELTPARLRDSDIDVVVLQRPEELELCERWTGRRPGRDVPAVFVEHNAPQGRVN
                     ELRHPLADRDDVTLVHVTHFNALFWDAGTTPITVIEHGIADPGHRWTGELPHAAVVVN
                     EPVRRGRVAGTDLLPRVAGAAPLELFGMGVAPLGGEAWCAAAHEDLPQERMHDEIARR
                     AVYLHPFRWTSLGLALIEAMHLGMPIVALSTTEAPEAVPPEAGVVSNRIDTLREGLRR
                     FTSDHEAAAAAGKAAREHALGRHGLRRFLDDWDRLLEEVTSR"
     gene            13661..14884
                     /locus_tag="VNU01_06885"
     CDS             13661..14884
                     /locus_tag="VNU01_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH32382.1"
                     /translation="MTTHLRIAMVSEHASPLAVLGGVDAGGQNVFVASLATALGRLGH
                     EVVVHTRRDDPALPERVAFARGVWVDHVDAGPPRPVPKDELLPYMDEFAKRLYTSWLG
                     DPPDVVHSHFWMSGKASLAAARPLGIPVAHTFHALGVVKRRHQGDKDTSPAGRLDTER
                     AIIAEADRVLATCTDEVFELTRLGAERSRVAVVPCGVDTARFTPEGPAEPRTPGFASR
                     IVCLSRMVERKGIGNVIEALAGVPGAELVVAGGPAKDELPADAEYRRLRDMAERHGVG
                     NRVDFRGRVERDDIPSLLRSADVVACTPWYEPFGIVPVEAMACGVPVVASAVGGMIDT
                     VLDGVTGIHVPPRDPARVARALNSLLGDERARKAFGAAGARRARARYSWERIAADTLA
                     AYPLRVAAQAQAVLS"
     gene            14881..15498
                     /locus_tag="VNU01_06890"
     CDS             14881..15498
                     /locus_tag="VNU01_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWH32383.1"
                     /translation="MSALDLRPALPTVAEHLDALREPLTALERDLARLERWGAHLACV
                     LLAGGRLLAAGNGGSAAQAQHLTSELSGRFRDDRRPYSAICLHGDSSALTAIANDYSV
                     EEVFARQVRAHGRPGDVLALLSTSGRSRNVLAAAAAGREAGMTVWGLTGTAPNPLARV
                     CDETISVQGASTATTQEVHQVVVHLLCLAAEPYLLDAEDREDAHA"
     gene            15491..>15886
                     /locus_tag="VNU01_06895"
     CDS             15491..>15886
                     /locus_tag="VNU01_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWH32384.1"
                     /translation="MREGRLVVVGDALLDRDVDGKAERLAPDAPVPVVDNPEWRARPG
                     GAALAAALAAADGRPVTLVTALGEDPAGRTVRLLLEECGVQVHDLGLRGRTPEKVRVC
                     AEGRPVVRMDLGGEPGEVGPATSGTGALLE"
BASE COUNT         2041 a         5884 c         5921 g         2040 t
ORIGIN      
        1 gcacgtacac cgactccagg ccgttgtcgt ggcggatctt caggtaatag ccgtagctcg
       61 gcatcggctc gccgatgccg gtcgcgacca ccacctcgcc cggcagcacg gcgtggacgc
      121 cctgcagctt gcggcccatc acgtcggtgg cctggtgcac gcgcccgccc gagcgcggcg
      181 cgtcgtaggt gtccgtgaag tccccgcgcg tgccgcccga gacgggaaag gtcaggtcca
      241 ccacgcgctc gtagccgtcg gggtcgccct cgacgcactc gcctcccggt tcggacggcg
      301 tgggcgacgg ctcctgggcg cccgcagaac cggcgggcgg caggacgagt acggcacagg
      361 acagcagcac ggcgagaaga cggctcatcg gccccatcgt gccgcaggcc ccgccggtgc
      421 gccgtcagcc ggcgacgatg tccccgccgg gatcgttgac ctcgatgccc tgggagcgca
      481 gccagtcctg ggcgtcgttg accgcggcct cgtcgccggt gagctccagg accatccagc
      541 cgacgtgctc gtccacgtcc gcgcggcgga tgttcacgtc cacgccatgg gtcttgacca
      601 tgtgggagat gatcggctgc tgcacgagtt gctcggggaa cgtcaggtgc agtcgcttgc
      661 tggtcatgga acacgcctcc ctcggccgcg gagcatcggg gggattgtgc cagcggccga
      721 gggaggcggt gggagcaagg tctcaggcgt ggtggggccg ccggaccgag cggtagatca
      781 gcagggcgat cacggcgccg atgatcgagc ccaggatccc ggtggcaccg agctccagct
      841 cgccgcggaa cagcagggcg ccgaggaaac cgccgacgaa ggagccgacg acaccgagcg
      901 ccacggtctc ccagaagctc atgggatcgc ggccgggcac gatcaggcgg gcgagaaagc
      961 cggcgatgat gccgatgacg agcagcgaca gcaacaggcc gagcatgtgt cctcctgggt
     1021 cggtggggcg tcgcccctac tgatacccag ggtcagcgcg ccgaaccgcc tcagccgccg
     1081 gcgaccgcgg gccaccggcg tgcttcgtcg cgccggagtc gcggcggcgt cagccgccgg
     1141 cgaccgcggg aatgatcgac acggtggcgc cgtcccgcac cggcgtgtcc aggccctgct
     1201 ggaagcggat gtcctcgtcg tcgacgaaca cgttcacgaa gcggcgcacc tggccgtcct
     1261 cgcccagcac ccgctcgccg atgccggggt gcgccgcgtc cagggccttg acgacctcgc
     1321 cgacggtggc gccctcgacg tcgacggtgg cttgctcccc cgtcaggttg cgcagggggg
     1381 tggggatgcg cacggtcacg ctcacgactc agctctcctt gtgttgcccg gcgcccagtt
     1441 cgagggccga ttggaagtct tgcagacccg cgttgatggt gtacgtcgga ccgacgcggt
     1501 cggcgacgac gtcgagcgtc ttcaggccca gcccgctgat gacggccacg gtgcgctcgc
     1561 cgcgctgcac gtggccctgc tcggcgagct tgcgcagcgt cgcgacggtc acgccgccgg
     1621 cggtctcggc gaagatgccc tcggtgcgcg ccaggagctc gatcgcgtcc acgatctccg
     1681 tgtccgagac gtcctccatc cacccgccgg tgcgccgcac cgcgtcgatc gcgtaatagc
     1741 cgtccgccgg gttgccgatc gcaagcgact tcgcgatcgt gtcgggcttc accgggcgca
     1801 cgaagccgct gccgtccttg agcgccgtgg agatcggcga gcagcccgtc gcctgggcgc
     1861 cggagacccg gaccggcttg tcgtccacca ggccgacctt caccagctcg ctgaacgcct
     1921 tgtcgatctt caccagcatc gagccggacg ccatgggggc gaccacgtgg tccggcagcg
     1981 tccagccgag ctgctcggcg atctcgaagc cgatggtctt ggacccctcg gcgtagtacg
     2041 ggcgcacgtt gacgttcacg aacgcccagc cgtactcccc cgccacctcg gcgcacagcc
     2101 ggttgacgtc gtcgtagttg cccttcacgg ccaccaggtt gggcccgtac acggcggtgg
     2161 acaggatctt ggcctgctcc aggtccgcgg ggatgaacac gaacgcgtcc atgccggcat
     2221 gggcggcgtg cgccgcgacg gagtgcgcca ggttcccggt ggacgcgcac gcggcggtgt
     2281 cgaagcccag ggcgcgggcg gccgacagcg ccacggacac gacacggtcc ttgaacgaga
     2341 acgtggggtt gagcgtgtcg ttcttcagcc acaggtcgtc caggcccaga gcggcgccga
     2401 gccgcggtgc cgggaccagc ggcgagaagc ccgcgcccag gtccacccgc tccgtcgggt
     2461 cgctgcccgg cagcacgggc aggaggtccg cgtagcgcca gatgctgcgc gggccctgct
     2521 cgatggactc gcgcgtgacg ttgccgcgca tgccgtcgta gtcgtagacg acctccagcg
     2581 gtccgaagca gaactcgcac acgtgcaccg gcgcgatcgc cgtctcgcgg ccacactctc
     2641 ggcagtgcag gcctcgcacg tagctcaccg tggctcctcc ttgcgtcctc gaaatcgaga
     2701 cagccctcca cgatgtggga gggctggtgc gcacggtggc ggccatctct cccctcgcgg
     2761 gggccggatt tcgcaccttc cctcggcgtg cgccttggtg gttgcggcgg tgtcgacggg
     2821 ccggatccct caaccgctct cgatggcgtt gcgatgaagt tgtcccgcaa cagtgacacc
     2881 gtcggcccgt ccgtgcaaat catgagcgtc gcgttaagac gcgcggcctg ccgtaaccat
     2941 cggtcgcctg cgggcgctgg ccgggcaagg gcacgaggcg tgcccttcgt ggcgtcgcgg
     3001 ccatttgggg ggccgggcgt tcacgcgacg acagaaaggt gggtcatgcg aaagctcttc
     3061 gcgatcgccg cggccgtgct gatgctcggg gccggcagcg ccctcgcaca gggaccggac
     3121 gccaacggtc cggccaagta cgggctgtgc acggcgtact tctcgggcca gggcggcgag
     3181 cacggcaacc ggaacgacgc gccgccgttc gccaacctcg aggccgccgc agaggaggcc
     3241 tacgagggtg acgaggacga cccgatcgag gacaaggtcc gtgacttctg cgacggcacc
     3301 cgcccgagca acggccgcgg caacggtcgt caggacgact ccagcgcgcc gaacccgatc
     3361 gactagcacc gcgcagcgat ccagcgggga gggcatctgc cctccccgct tttccgttgc
     3421 ccgcgcgtgc cgtgcatgat cggccgcatg tccaccgacg agttgggaca gcggctggcg
     3481 gcgctgccgc gcgtccgcgc gatctacacc gacctggacg gcacgctgct gggcccgcgc
     3541 gggtcgctgc tgcacggccc cgacggccgg ccgtccgcgc gtgcggccga ggcgctcgtc
     3601 gccgccgccg cggcgggcgt cgaggtgatc ccggtcagcg ggcgccagcg caagcagctg
     3661 gagaacgacg cccgcctgat ggggctggac gactgcgtcg cggaggccgg cgtggtgatc
     3721 gtgcgcgacg gcgtgcgcca ctacgagtgg ggcgacctcc cgcgcggcct ggccggcaac
     3781 ccgcacgaca ccatgacgga ggccggcgcg gtcgccgtgc tgctgcgcgc cttcccggac
     3841 gacctgcgcc actacgagcc gtggcacctg gaccgggagg gcagccatct gttccacggg
     3901 cgggtcgacg tggcggcggc cgaccgcctg ctggacgagc acggctgctc ctgggcctca
     3961 ctgatcgaca acggcgacac cggcgggtgg ccggggcgca gcgtgcgcgc gtaccacctg
     4021 gtgccgcgcg gcatcgacaa ggcgcgcgcc gtcgccgacg acatccaggc acggggcctg
     4081 cgtcgggagg agacgctcgg tgtcggcgac agtctggccg acctggcgat ggcggcgtcc
     4141 gtcggcacgt tcgtcatcgt ggcgaacggg cacggcgagg cgggcgacgg cgtggtgcgc
     4201 gtggacgagg cgatgggcca cggtttcgcg gccgcggtgg aagccgcgct cagcgcccgg
     4261 cgctgaccca ctgcggagtc aattgcggcc gctatcggac ggaatcgact ccgcagcggc
     4321 ggggaggtga tgctggcgct cggcgcgaag ccagggaacg cgcgtgcgcc agaagtgcac
     4381 gatcaccacg ttgacggcgg cgaccagggg gatcgcgacg agcatgccga agatgccgta
     4441 gagcgtgccg gccaccagca gcgccagcag caccgcaagc gggtgcagct gcacgttgcg
     4501 gccgaccacc aggggcgaga cgacgtggcc gtcgagctgc tgcaccaccg tgacgaccag
     4561 cacgaccaag agcgccagcg cggggccttc cgtcaccagg gcgatggtga cgccgacgag
     4621 cccgccgacg aacggcccga tcagcgggac gaggttggtg acccccgcga ggatcccgac
     4681 cagcagccag aagcgcaggc cgatcgccca caggccgatg ctggtcgcca tgccgacgaa
     4741 cgtcgcgacc agcagctggc cgcgcacgta gccgccgacg acgacggcga gcttgcgcgc
     4801 cagcacgtcg gcctcgtcgc ggtgccgcgg gggcagcgca ccatgggccc agcggcccag
     4861 ccgcggcagg tccaccagca cgtagaaggc gacgaacggc ccggcgatga acaccaccag
     4921 caggtgcacc aggcccatgg ccacgccgga gatgccgccc agcagcgcca gcacggcctc
     4981 gcgattgcgc tcgtcggaca ggaagcgctc gacctcctcg ctgatgcggt cgccggacag
     5041 gctctccgac acgcgcacgt tggcgcccag gcgggccagg aggtcgttca cgcccgacac
     5101 cagccggttg cccagctgcg gcaggtcgtt ggcgaagttg cgcacctgct cggccagcag
     5161 cggcgacagc acggcgacca gcacgccgat ggtcagcgcc attgtgaggt aggcgatgcc
     5221 gactcccgcc cagcgggcga tgccgcggcg ctccagccac atcaccagcg ggttgagcac
     5281 gtacacgatc agcatcgcca gcaggatggg cggcagcagc accccgaccg gctcgcgcag
     5341 cagccagaac agccctacca gcagaagcag cgaacccacg gccagccaga ccgctgtgtt
     5401 cgcgcgctgc aggatggtgc gctccatcgc agcagggtac gccgtggccc gcccgatgcg
     5461 tcctagctgt ccgcgaccca gtcgcgcccg atcacgacgt gcaggtcgat gctcgcgtcc
     5521 agccggtcgt tgcgctccag cacgccgaag cggtcgtcgg tgtcctgcag ccgctcggcc
     5581 tcgtcctcga agccggccga ccagaacacg gtggtctcgt cgtagccgcg cgccgcccgg
     5641 ctctgcgcca ccacctggta gcccagctcc tccaggacct cgacgacgtc gccgagcttg
     5701 tccgggtcgc cggaggcgtt cagcacctgc acagtggcgc gcacggggcc gtcgtcgccg
     5761 ccgtccgccg tggcctcggg gccctccacc gccggctcgt cctggaagcc ggtcggcgtg
     5821 ggggtgggcg aggaggtcac cgtgtcggcg ggcgccggct ccccggtgtc gtccagggtg
     5881 accaccggct tctcaccttc gtcgccgccg cccagccagc gcaccaggcc ggccgcggcc
     5941 agccccacca gggcgacggc gagcagtgcc ttcccggcgg ccgcggccag cgacagccag
     6001 aaccagcgcg gatcgggatc ggcgtggcgg cccatgcctc agcggcgctc gcgcacggcc
     6061 tccgccgtgc ggcggcggtc cagccggcca cggcgtcgcc gcaggcgcag cacggtcagc
     6121 gggtcgtagg cgtacgcctc ggccgagtcg atcagcttgg ccagaagctg gtggtagcgg
     6181 gccttgctga agccgaaggc ctccttgatg gcctgctcct tgccggtctc ggcgccggcg
     6241 gccggccact cgcgctcgaa gtcgagcagc gcgcggctcc tcgcgtccag cccgccctcg
     6301 ggcgggagcg gctgacccat gactcgtcct tcgcactcga cgtgggcgtc cctggccgtg
     6361 ccgggggcct gggccgtcac gtggatccgc cgccgagtgt aggcaggggc acccggcatg
     6421 ccgtggaacc cggacagccg ccgttgtgtc tagcctgtgc ggccgagacg ggcacacgac
     6481 cgcggaggac gcatggccga gtcattgcgg ggcaaggtcg cactggtcac cgggggcgcc
     6541 gggggcttcg gcgccgcgat ggggcgccgc ctggcggcgc gcggcgcgca ggtcgtcctg
     6601 gccgatgtcc acgaggaggg cgccgcggcc gtggccgagg agatcggcgg cgcggcggtg
     6661 cgctgcgacg tgtcgtcgct ggaggacaac ctcgccgccg tcgcgttcgc ggtggagcgc
     6721 ttcggcgggc tggacatcgt ggcgctgaac gcgggggtga gcaccggctt cggcatcgag
     6781 gacgacttcg acgtcgacaa gtaccggcgc gtgatgggca tcaacctcga cggcgtggtc
     6841 ttcgggctgc acgccgcgct gccgcggctg cgcggccgcg gcggcggcga cgtgatcgcc
     6901 acggcgagcc tggccggcct gacccccgtg ccgatggacc cgctgtacgc ggccaacaag
     6961 gcggcggtgg tgcacctggt gcgctcgctg ggtcccgtgc tcggcgacag cggcatccgc
     7021 gtgaacgccc tgtgcccgtc gttcgcccac accccgatca tcacgccgat ccgcgaggcc
     7081 ctggagggca tgcgcacgcc gatcctgccg gtggacgcgg tcatggacgc cttcgagcgg
     7141 atcctggact ccggccagag cggccaggcc tggttcgtcc agtacggcag gcccagcgag
     7201 ccgttcggct tccgcggcgt gccggggccc cgcgtcgagc agtagcgccg ctcaggacac
     7261 cggcgtcgcc ggacggcccg tgccgccgag caccgagcgg taccactcga tggtgcgggc
     7321 caggccctcc cgcagcggca ccgcaggctc ccagcccagg cgcgtccgcg ccagcgtgat
     7381 gtccgggcgc cgctgcgccg ggtcgtccgc cggccgcggc acgaggcgca cctcgctggg
     7441 cgagtcgcac atccgcacca ccagctccgc cagctcgcgc accgtgtgct cgtccggatt
     7501 gccgatgttg accggcccgt cctcctgcga gcacagcagg cggaacagcc cgtccacgat
     7561 gtcgtcgacg tagccgaagg agcgcgtctg cccgccgtcg ccgtgcacgg tgatcggctc
     7621 gccgcgcagc gcctggccga cgaacgtggg gatggcacgg ccatcgttgg ggcgcatgcg
     7681 cggtccgtac gtgttgaaga tgcgcgcgat gcgcgtgggc acgccgtgcg tgcggtggta
     7741 ggccagcgtc atcgcctcgg cgaagcgctt cgcctcgtcg tacacgccgc gctcgccgat
     7801 cgggttgaca ttgccccagt agtcctccgg ctgcggatgc accagcgggt cgccgtacac
     7861 ctcgctggtg gacgccagca tgaacaccgc gcgcttctcg cgcgccaggc ccagcgcctt
     7921 gtgcgtgccg aggctgccga ccttcagcgt ctggatcggc agggcgcggt agtcctccgg
     7981 tgaggccggc gaggcgaagt gcagcaccgc gtccacgtcg ccgggcacgt gcaggaactc
     8041 ggtgacgtcc tggcgcacga agcgcagcag gcggctgccc tgccacgcct gcaggttctc
     8101 caagcggccg gtcaccaggt tgtcgagcac cgtgacccgc cagccctcgc gcagcaggcg
     8161 gtccacgagg tggctgccga ggaacccggc gccaccggtg atcacggcgt gctggaccac
     8221 gcgccttcct ttcgtcggcg ggagcgtcgt gcctgcagct accccgcacc ggcccagcct
     8281 agtcgcaaag gaaaacgcgg ccggtgtgat catcggaacg tagttcgctt cctgtttttt
     8341 ttcacggccg tcggcgcatg agcaccgctt ccctcgggca ctcgtgacaa cccgtcagga
     8401 acacaggagt cccgtgtatg cgagtgctgg gattgaacgc cgtcttccac gacccggcgg
     8461 ccgcgctggt cgtggacggg gaggtcctcg ccgccgccga ggaggagcgc ttcagccgcc
     8521 gcaagcacgg caagccgtcc gtgccgttct ccacctggga gctgcccgag cagtccgcca
     8581 agtggtgcct ggcgcaggcc ggcctttcca tcggcgacat cgacgccatc gcgtactcct
     8641 atgacccggc gctggccacg ccgcgtggcg agcgcgtcga ggacctcgtc gccggcgggt
     8701 gggacggcct gcgcaccctg tacgcgcagc gcgccccgct gttcctgcgc acgctgttcc
     8761 ccgagctgga gccggagcag gtccgcttcg tgccccacca cgtcgcccac gccgcctcgg
     8821 cccacctggc cgcacccttt ccgacctcct ccgtgctggt cctggacggg cgcggcgagc
     8881 gctggagcca cctggccggc cgggcgctgg acggcgagct gaaggtgctg gcgcagcagg
     8941 aactgccgca ctccctgggc ctgctgtacg aggagctcac cgagcacctg ggcttccgcc
     9001 gctccagcga cgagtacaag gtcatggcga tggcctccta cgggaggccg cgcttcctgg
     9061 gcgagttccg gcggctggtc cacgccgacg gcgagggcgg cttccgcgtg gagccgatcg
     9121 actggagcgc gttcgcggcg cgccgctcgc cccgcacgga ggacttcggc gaggaccacg
     9181 ccgacctcgc ctccaccgtg cagacgcgcc tggaggaagt gctgctggac ctggcgcgct
     9241 ggctgcacgc caccaccggc gacaaggtgc tcaccatggc cggcggcgtg gccctcaact
     9301 gcgtggccaa cgcgcgcctg gccgccgagg gcccgttcga gcacatctgg gtgcagccgg
     9361 ccgcgggcga cgccggcacg gcgcttggcg gcgcgctgca cctggcccag gcgctgggcg
     9421 acgacacgct ccccatgacc ggcgccgacc ttggccgcgg ctggaccgac gacgagctgg
     9481 aggcctggct caccaccgcc gaggtgccct acgagcgtcc cgccgacgtc gccgaggcgg
     9541 tcgccgactg cctggcggac aacggcgtgg tggcgtggtt ccagggccgc agcgaatacg
     9601 gcccgcgcgc actgggccac cgcagcctgc tcgcggaccc gcgccgcgag gagaacctgc
     9661 ggcgcctgaa cgaggtgaag ggccgcgagc agttccggcc ggtggccccc atggtgctgc
     9721 tggagcgggc cggcgagatc ttcaccggcg tcatcccgag cccgtacatg ctgttcacgc
     9781 acggcgtgcg gccggagtgg cgcgagcgca tcccggcggt ggtgcacgtg gacggcaccg
     9841 cgcgcatcca gacggtggac cgcgacagcg agccgctggt ggcgcgcatg ctggagggct
     9901 tcgcgcggcg caccggcgtg ccggtggtgg tcaacaccag cctcaacacc gcggggcgcc
     9961 ccatggtcga cgacccgcgg gacgcgctgg agtgcttcgg ctccggcccg gtcgacctgc
    10021 tggcgctggg accgttcgtg gtgcgccgcg gcgcgatgtt cgccgcgggc cccgggggcg
    10081 gccgatgagc gctcccgcgc ccgcgccgtc gttcgacgtg gtggtcccca ccatcgggcg
    10141 gccatccctg cgcgcgctgc tggagtcgct cggcgcctgc gacggcccgc gcccgcggcg
    10201 gatcttcctg gtggacgacc gcgccgacca gcgcgcgccg cttgacgtcg gcgcgccggc
    10261 gccgtgggcg cgcgagcgcc tggccgtgct gccggggcgc gcggccgggc ccgccgccgc
    10321 gcgcaacgcc ggctggcgcg ccgcccgcgc cggctgggtc gcgttcctgg acgacgacgt
    10381 ggtggtgccg ccggagtggc tggcgcgcct ggccgcggac ctgcacggcg ccggcccgga
    10441 cgtgggcggg gtgcagggcc gcatcaccgt gccgctgccg gccgggcggc gcccgaccga
    10501 ctgggagcgc aacgtcgccg gcctggaggc ggcgcagtgg gcgaccgccg acctggccta
    10561 ccggcgcgcc gcgctggagt ccgtcggcgg cttcgacgag cgcttcccgc gcgcctaccg
    10621 cgaggacgcc gacctgggac tgcgcgtcac ggagtcgggc tggcgcatcg tgcgcggcgc
    10681 gcggcgcatc acgcaccccg tgcggccggc ggactcccgc gtgagcgtgc gcctgcagcg
    10741 cggcaacgcc gacgacgtcc tcatggcggc gctgcacggc aagggctggc gcgagcgcgc
    10801 cggcgtgccg cgcgggcgcc gccgggcgca cctggccacc gccgggctcg gcgccctgac
    10861 ggcgctggcg gccggccgcg ggcgccgccg ggcggcggcc gccgcgggcg ccgggtggct
    10921 ggtgctgacc gccgagttcg ccgcgcgtcg catcgcgccg ggcccgcgcg accgcgcgga
    10981 gatcgccacg atggcgtgga ccagcgcggc catcccgttc gccgcgtcgt ggcactggct
    11041 gaccggctgg gcgcgcctgc cgcggcgcct cgcggagccg ggcccgatcg cgggcgcggt
    11101 gacgtcgccg ctgcccgacg cggtgctgct ggaccgcgac ggcaccctga tccgcgacgt
    11161 gccctacaac ggcgaccccg gccgcgtcgt gccgatgccc ggcgccgccg aggccctggc
    11221 gctgctgcgc cgggcgggcg tgcgcgtggc ggtggtgtcc aaccagagcg gcgtcgcccg
    11281 cggcctgctc acccgcgagc aggtcgacgc ggtgcacgcg cgcgtggaag agctgctcgg
    11341 cccgttcgac ggctggttcg tctgcccgca cggccccgac gatggctgcg cctgccgcaa
    11401 gccggcgccg gggctggtca aggaggccgc cgccgcgctg ggcacggaac cggagcgctg
    11461 cgcgctcgtc ggcgacatcg gcagcgacgt cgacgcggcg ctggccgccg gagcccggcc
    11521 catcctcgtc ccgactcccc agaccctgga gacggagatc gccggcgcgc ccgaggtggc
    11581 gcgcgacctc accgacgcga tcgcccgcat cctcaggggg agccgatgac cgcaacgcac
    11641 atcctcgccg tgcggctgga caacgacggc gacgtcctgc tggccggccc cgcgatccgc
    11701 gccctggccg cctccggcgc ccgcgtcacg ctgctgtgcg ggccgcgcgg tcgccgggcg
    11761 gcgcgcatcc tgccgggcgt ggacgccgtg cacgtgttcc aggcaccctg gatcgacccg
    11821 gagccggacg ccgtgcgccg cgacgacatg gacgcgttcg tggacggcgt cgccgcgctc
    11881 cgcgctgacg aggcgctggt gttcacgagc ttccaccaga gcccgctgcc cacggcgctg
    11941 ctgctgcgca tggccggcgt ggcgcgcatc ggcgcgatca gcgaggacta cccgggctcc
    12001 ctgctggacc tgcgccatcg cgtgccccac gacatccacg aggtcgagcg cggcctgtcc
    12061 ctggccgcgg cgttcggcta cgcgccaccg gccggcgaca acggccgcct gagcgtgcgc
    12121 cgcaccggcg ccacgccgcc gccgttcgac gagccgtacg tggtggtgca ccccggcgcg
    12181 tccgtcccgg cgcgcgcctg gccgcccgcc caccaccgcg agctcgtgcg cctgctcgcg
    12241 tcccgcgggg tccccgtggt ggtgaccggg tcgccgtcgg agcgggagct cacgcgcgag
    12301 gtcgccggcg gcccgtcgga cttcgtcgcg gaccttggcg gcgcgaccac gctgggcgag
    12361 ctggccgacg tgctcgcgga cgcgagcgcc gtcgtggtcg gcaacaccgg gccggcgcac
    12421 ctcgccgccg cggtcggcac gccggtggtg tccctgttcg cgcccacggt gccgcccatc
    12481 cgctggcgtc cctacaaggt gccgcagcgg ctgctgatga ccgacgtgcc gtgcgccggc
    12541 tgccgtgccc gcgaatgccc cgtggcgggc cacccgtgcc tgagccagct gacgccggaa
    12601 atggtgctgg gcgcgctcga cagcctgctc gcggagaccg ccgccgcgcg gccgcaacgc
    12661 cgcagccgcg ccgccagggg cctgggcagc ctggtcgagg tggggccatg aggatcctgc
    12721 actggcacgt ccacggctcc tggaccacgt cgttcgtaca cggcccgcac acctacctgc
    12781 tgccggtgct gccggaccgc ggcccggacg ggcgcggccg cgcccgcacc tgggactggc
    12841 ccgccagcgc ggtggagctc acgcccgcgc ggctgcgcga cagcgacatc gacgtggtgg
    12901 tcctgcagcg cccggaggaa ctggagctgt gcgagcgctg gaccggccgg cgccccggcc
    12961 gcgacgtccc ggccgtgttc gtggagcaca acgcgccgca gggccgcgtg aacgagctgc
    13021 gccacccgct ggcggaccgc gacgacgtca cgctggtgca cgtcacgcac ttcaacgcgc
    13081 tgttctggga cgccggcact acgcccatca ccgtcatcga gcacggcatc gccgacccgg
    13141 gccaccgctg gaccggcgag ctgccgcacg ccgccgtggt ggtgaacgag ccggtgcgcc
    13201 gcggccgcgt cgccggcacg gacctgctgc cgcgcgtggc gggcgccgcg ccgctggagc
    13261 tgttcggcat gggtgtggcg ccgcttggcg gcgaggcgtg gtgcgcggcg gcgcacgaag
    13321 acctgccgca ggagcgcatg cacgacgaga tcgcgcggcg cgccgtgtac ctgcacccgt
    13381 tccgctggac gtcgctcggc ctggcgctga tcgaggccat gcacctgggc atgccgatcg
    13441 tcgcgctgtc caccacggag gcacccgagg cggtgccccc ggaggcggga gtcgtgtcga
    13501 accgcatcga cacgctgcgc gaagggctgc gccgcttcac ctcggaccac gaggcggcgg
    13561 ccgcggccgg gaaggccgcc cgtgagcacg ccctggggcg ccacgggctg cggcggttcc
    13621 tggacgactg ggaccggctg ctcgaggagg tgaccagccg atgaccacgc acctgcgcat
    13681 cgcgatggtg tccgagcacg cgagcccgct cgcggtcctg ggcggcgtcg acgccggcgg
    13741 ccagaacgtc ttcgtggcgt ccctggccac ggcgctgggc cggctgggcc acgaggtcgt
    13801 ggtgcacacg cgccgtgacg accccgcgct gcccgagcgc gtggcgttcg cccgcggcgt
    13861 gtgggtggac cacgtcgacg ccggcccgcc ccggccggtg cccaaggacg agctgctgcc
    13921 gtacatggac gagttcgcca agcgcctgta cacgtcctgg ctcggcgacc cccccgacgt
    13981 cgtgcacagc cacttctgga tgtcgggcaa ggcctccctg gcggcggcgc ggccgctggg
    14041 catcccggtc gcgcacacgt tccacgcgct gggcgtggtg aagcgccgcc accagggcga
    14101 caaggacacc agccccgccg ggcggctgga cacggagcgg gcgatcatcg ccgaggccga
    14161 ccgcgtcctg gccacctgca ccgacgaggt gttcgagctc acgcgcctcg gcgcggagcg
    14221 ctcgcgcgtg gccgtggtgc cctgcggcgt ggacacggcc cgcttcacgc ccgaaggccc
    14281 ggccgagccg cgcacccccg gcttcgcgag ccgcatcgtg tgcctgtcgc gcatggtgga
    14341 gcgcaagggc atcggcaacg tgatcgaggc gctggccggc gtgcccggcg cggagctggt
    14401 ggtggcgggc ggccccgcca aggacgagct gcccgcggac gcggagtacc gccgcctgcg
    14461 cgacatggcc gagcgccacg gcgtgggcaa ccgggtcgac ttccgcgggc gggtggagcg
    14521 cgacgacatc ccgtcgctgc tgcgctccgc cgacgtggtg gcgtgcacgc cgtggtacga
    14581 gccgttcggc atcgtgccgg tggaggccat ggcctgcggc gtgccggtgg tcgcctccgc
    14641 cgtcggcggg atgatcgaca cggtcctgga cggcgtcacc ggcatccacg tgccaccgcg
    14701 cgacccggcg cgcgtggccc gggcgctcaa ctcgctgctg ggcgacgagc gcgcgcgcaa
    14761 ggccttcggg gcggccggcg cgcgccgtgc ccgcgcgcgc tactcgtggg agcgcatcgc
    14821 ggcggacacg ctggcggcct acccgctgcg cgtggcggcc caggcccagg cggtgctgtc
    14881 atgagcgcgc tggacctgcg gcccgcgctg cccaccgtgg ccgagcacct ggacgcgttg
    14941 cgcgagccgc tcacggccct ggagcgcgac ctggcgcggc tggaacgctg gggcgcccac
    15001 ctggcgtgcg tgctgctggc cggcgggcgc ctgctggccg ccggcaacgg cggcagcgcc
    15061 gcgcaggcgc agcacctcac cagcgagctg tcgggccgtt tccgcgacga ccgccgcccc
    15121 tactcggcga tctgcctgca cggtgactcc tcggcgctga cggccatcgc caacgactac
    15181 tccgtcgagg aggtgttcgc ccgccaggtg cgcgcgcacg ggcgtcccgg cgacgtgctg
    15241 gcgctgctgt ccacgtccgg ccgcagccgc aacgtgctgg ccgccgcggc cgccgggcgc
    15301 gaggccggca tgaccgtgtg ggggctgacc ggcactgcgc ccaacccgct ggcgcgggtg
    15361 tgcgacgaga ccatcagcgt gcagggcgcc agcaccgcca ccacccagga ggtccaccag
    15421 gtggtcgtcc acctgctgtg cctggccgcc gagccgtacc tgctggacgc cgaggaccgg
    15481 gaggacgcgc atgcgtgagg gccgcctggt cgtggtgggc gacgcgctgc tggaccgcga
    15541 cgtggacggc aaggccgagc gcctggcccc ggacgcgccg gtgccggtgg tcgacaaccc
    15601 cgagtggcgc gcgcgtcccg gcggcgccgc gctcgcggcc gcgttggccg ccgcggacgg
    15661 acggccggtc acgctggtca cggcgctggg cgaggaccct gcgggccgca cggtgcgcct
    15721 gctgctggag gagtgcggcg tgcaggtgca cgacctgggc ctgcgcggcc gcaccccgga
    15781 gaaggtgcgc gtgtgcgccg agggcaggcc cgtggtgcgc atggacctgg gcggcgagcc
    15841 gggcgaggtg gggcccgcga cctcggggac cggcgcgctg ctggag
//
LOCUS       DATMKC010000235         3215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_353345, whole genome shotgun sequence.
ACCESSION   DATMKC010000235 DATMKC010000000
VERSION     DATMKC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353345"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VNU01_06900"
     CDS             <1..725
                     /locus_tag="VNU01_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWH32385.1"
                     /translation="CALLGLDSLDEAVALLPRLRELDSLEAAEVMFDRGVHLVAMHAG
                     LPLPFEPLPPVLLLVECAGPRDPLEELAAALEGLPRVAVAVERAERERLWAYRERHTE
                     AINAFGVPLKFDVAVRLPELASTVHELEGDLEEAWPDAIVVLFGHLADGNMHINVLPP
                     ESGTPMLGSRAATAEDVEALVLRRVIDVGGAISAEHGIGVAKRDWLVQSRHPSELAPM
                     RAIKRALDPGNLLNPGVLFPPD"
     gene            complement(722..1660)
                     /locus_tag="VNU01_06905"
     CDS             complement(722..1660)
                     /locus_tag="VNU01_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32386.1"
                     /translation="MRPRTPLAIVLLLGLAVAGCASGDGTDTATETQTATPSPTSTAT
                     SGEVDEQALEAVAEAAQATVDEDTARFTLTVATEGTGTDGQAPVTAEGEEDFAEERRL
                     FTFEGPQGELRIIVDGDTAYIEMPATEEERWAELELDRLSGGDFGFGGPGGIPFQGVQ
                     DNVEFLRDSAVRAEQAGEDDVDGEPAIRYELTVDLEESAEDAPEDTERGLRRTLRETG
                     VRTLDVTVWVDEDGRVARIEYALDLGQADIDEEGVEADPRGRAIVTVDYRDFGADLSI
                     DVPTGDEVVEMDEDELRQSFGGDSPTGTPEPDETET"
     gene            complement(1729..2367)
                     /locus_tag="VNU01_06910"
     CDS             complement(1729..2367)
                     /locus_tag="VNU01_06910"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HWH32387.1"
                     /translation="MRWLIGLLLVLAVVAVAADQVVAAEVEQRAEARLAERLGTETDV
                     ELHGWPVGLRVLLGQVPRLTAHAEDVRVEGGVRIARLDAELTEVSFDRSAARLPRAAT
                     AEFTAVLTEQALRDALGAPEQLVSLSLRDGIAVLDVAGLQVEADVVARDGAVVVEPRD
                     ALSALLGAGGLRVDLSDQPGAPAAREVEVQEGRLVVRGVLEGLVGPEGAGGE"
     gene            complement(2426..>3215)
                     /locus_tag="VNU01_06915"
     CDS             complement(2426..>3215)
                     /locus_tag="VNU01_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWH32388.1"
                     /translation="VAKFRAARRIWARVMRDRFGATDPKSQMLRFHTQTAGVQLTAQQ
                     AEVNLIRVTIQSLAATLGGTQSLHSNAFDEALALPTEKSALLALRTQQVIAHETDVTY
                     TADPLGGSWFIESLTDEIERQALDYIAKIDDMGGAVRAIEQGFQKSEMEHSSYEIAKA
                     IEREEQIVVGVNRYRVEEEIQPELQRVDEAVRDGQVERLAKVRAERDQDAVDEALRAV
                     AEAAKGTDNLLPPMREALRRMATIGEICDTLRGVFGRYQPVETL"
BASE COUNT          387 a         1212 c         1145 g          471 t
ORIGIN      
        1 tgtgcgcact gctcgggctg gacagcctcg acgaggccgt cgcactgctg ccccgcctgc
       61 gggagctgga cagcctggag gccgccgagg tgatgttcga ccgcggcgtg cacctggtcg
      121 cgatgcacgc gggcctgccg ctgccgttcg agccgctgcc gccggtgctg ctgctggtcg
      181 agtgcgccgg cccgcgggac cctctggagg agctggcggc ggcgctggag ggactgccgc
      241 gcgtcgcggt cgcggtcgag cgcgccgagc gcgagcggct gtgggcctac cgggagcggc
      301 acaccgaggc gatcaacgcc ttcggcgtgc cgctcaagtt cgatgtcgcg gtccgtctgc
      361 cagagctggc gtccaccgtc cacgagctcg agggcgacct cgaagaggcg tggcccgacg
      421 cgatcgtggt gctgttcggc cacctcgccg acggcaacat gcacatcaac gtcctgccgc
      481 cggaaagcgg aacgcccatg ctcgggagtc gcgccgccac ggcggaggac gtcgaggcac
      541 tggtgctccg ccgtgtgatc gacgtcggag gagcgatcag cgccgagcac ggcatcggcg
      601 tggccaagcg cgactggctg gtgcagtccc gccacccctc cgagctcgcg ccgatgcggg
      661 cgatcaagcg cgccctggac ccgggcaacc tgctcaaccc aggcgtgctg ttcccgccgg
      721 actaggtctc cgtctcgtcc ggctccggcg tcccggtggg cgagtcgccg ccgaaggact
      781 ggcgcagctc gtcctcgtcc atctcgacca cctcgtcacc cgtcggcacg tcgatggaca
      841 ggtcggcgcc gaagtcgcgg tagtccaccg tgacgatggc gcggccgcgc gggtccgcct
      901 cgacaccctc ctcgtcgatg tcggcctggc cgaggtccag cgcgtactcg atgcgggcga
      961 cgcgcccgtc ctcgtcgacc cacacggtga cgtccagcgt ccgcacgccg gtctcgcgca
     1021 gcgtccggcg caggccccgc tcggtgtcct ccggggcgtc ctcagccgac tcctccaggt
     1081 ccacggtgag ctcgtacctg atggccggct cgccgtcgac gtcgtcctcg ccggcctgct
     1141 cggcgcggac cgccgagtcg cgcaggaact cgacgttgtc ctgcaccccc tggaacggga
     1201 tgccgcccgg gccgccgaag ccgaagtcgc cgccgctcag gcgatccagc tcgagctcgg
     1261 cccagcgctc ctcctccgtg gcgggcatct cgatgtaggc ggtgtcgccg tcgacgatga
     1321 tgcgcagctc gccctgcggt ccctcgaagg tgaacagccg ccgctcctcg gcgaagtcct
     1381 cctcgccctc ggcggtgacg ggagcctggc cgtcggtgcc ggtgccctcc gtcgccacgg
     1441 tgagcgtgaa gcgggcggtg tcctcgtcga cggtggcctg cgccgcctcc gccaccgcct
     1501 ccagcgcctg ctcgtcgacc tcgccggagg ttgcggtgct ggtgggcgag ggtgtggccg
     1561 tctgcgtctc cgtcgccgtg tccgtcccgt cgccggaggc gcagcctgcc acggccaggc
     1621 ccagcagcag gacgatcgcg agcggcgtgc ggggtcgcat gggggctctc ctggcggttc
     1681 ttcaggggcg gtgggcgtcc gtgaggggct acccctgcgc gcgcggcgtc attcgcctcc
     1741 ggcgccttcc gggccgacca gcccttcgag gacgccgcgc accaccagcc gcccctcctg
     1801 gacctcgacc tcgcgagcgg cgggagcgcc gggctggtcg gacaggtcga cgcgcaggcc
     1861 gcccgcgccg agcagcgccg acagggcgtc gcgaggctcg acgaccacgg ccccgtcccg
     1921 cgcgacgacg tcggcctcca cctgcagccc cgcgacgtcc agcaccgcga tgccgtcccg
     1981 caggctcagc gagacgagct gctcgggtgc gccgagggcg tcgcgcagcg cctgctcggt
     2041 gaggaccgcg gtgaactccg cggtggcggc gcgcggcagg cgcgcggccg accggtcgaa
     2101 ggacacctcg gtcagctcgg cgtccagtct cgcgatgcgc acgccgccct ccacgcgcac
     2161 gtcctccgcg tgcgcggtca ggcggggcac ctggccgagc agcacccgca ggcccacggg
     2221 ccatccgtgc agctccacgt ccgtctcggt gcccagccgc tcggccaggc gcgcctcggc
     2281 gcgctgctcg acctcggccg ccacgacttg gtcagcggcg accgccacca cggccaagac
     2341 gagcaggaga ccgatcagcc agcgcatggg acaccttcga gtcggtcgcc gggccggcga
     2401 gccggcctcg gcgaggagcg agcggctaca gcgtctccac cggctggtag cggccgaaca
     2461 cgccgcgcag cgtgtcgcag atctcgccga tggtggccat gcgccgcagc gcctcgcgca
     2521 tcgggggcag caggttgtcg gtgcccttgg cggcctcggc cacggcgcgc agcgcctcgt
     2581 cgaccgcgtc ctggtcgcgt tcggcgcgca ccttcgccag gcgctccacc tggccgtcgc
     2641 gcaccgcctc gtcgacgcgc tggagctccg gttggatctc ctcctcgacg cggtagcggt
     2701 tcacaccgac gacgatctgc tcctcccgct cgatcgcctt ggcgatctcg taggaggagt
     2761 gctccatctc cgacttctgg aagccctgct cgatggcgcg caccgcgccg cccatgtcgt
     2821 cgatcttggc gatgtagtcc agcgcctggc gctcgatctc gtcggtcagc gactcgatga
     2881 accagctccc gcccagcggg tcggcggtgt aggtgacgtc cgtctcgtgg gcgatgacct
     2941 gctgcgtgcg cagggccagc agcgccgatt tctccgtggg cagcgccagc gcctcgtcga
     3001 acgcgttgga gtgcagcgac tgggtgccgc cgagcgtcgc ggccagcgac tggatcgtca
     3061 cccggatgag gttgacctct gcctgctgcg cggtgagctg caccccggcc gtctgcgtgt
     3121 ggaagcgcag catctggctc ttggggtcgg tggcgccgaa gcgatcccgc atgacgcgcg
     3181 cccagatgcg ccgtgccgcg cggaacttcg ccacc
//
LOCUS       DATMKC010000236         2063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_193480, whole genome shotgun sequence.
ACCESSION   DATMKC010000236 DATMKC010000000
VERSION     DATMKC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193480"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VNU01_06920"
     CDS             complement(<1..1014)
                     /locus_tag="VNU01_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32389.1"
                     /translation="MSTSDAAAAFSTLADLQRGMAARGGGPGPSVALPEAAPTLPLKV
                     ERTRTFDTPEFAGMTFLEVEAKSALNRVQGMPFKWSINPYRGCSHACAYCVGGDTPIL
                     MADGRLKPIAEVRSGDRVYGTERRGAYRRYVVTEVRDQWSTMKEAYRIRLEDGTTLVA
                     SADHRFLTERGWKHVAGAGRGPDCRPHLTTNNSLRGFGGFAAPPKQDEDYRRGYVHGL
                     ISVVGSGTVDAPAGRRFVEILERDVKPVPGRELAHLMGWPDEASDEWRKGLLAALFDV
                     AGSHDGKSLRLRANKSRVLLEAQKCLWALGFRYEAEAGASPSWVRVAGGPREHLRFFH
                     TVEP"
     gene            complement(1048..1917)
                     /locus_tag="VNU01_06925"
     CDS             complement(1048..1917)
                     /locus_tag="VNU01_06925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH32390.1"
                     /translation="MSERVTVVVATQNRRGGLADALERLAGLSPASPVIVVDNASTDG
                     TAQMVRARFPGVRVVVLPRNIGAAARNAGAREAATDLVAFSDDDSWWAPGALARAAEH
                     FDAHPRLGLLAARMLVGAREELDPVCDAMAASPLGTEPDLPGPSVLGFVACGAVVRRD
                     AFLQAGGFHERFLVGGEEELLALDLATAGWGLAYVDDVVAHHHPSPSRNPSRRRRIQR
                     RNALWAAWLRRPLSAVAGHTLAALGAGLRDADARAGLGEALTRGAWVLRERRVVPAGV
                     ERRVRRLERGKGA"
BASE COUNT          309 a          792 c          695 g          267 t
ORIGIN      
        1 gggttccacc gtgtgaaaga agcgcaggtg ttctcgggga ccgccggcga cgcgcaccca
       61 cgagggggac gcaccagctt cggcttcgta ccggaagccc aacgcccaca ggcacttctg
      121 cgcttcgaga agcactctcg acttgttcgc ccgcaagcgc agactcttcc cgtcgtgcga
      181 gcccgccacg tcgaacagtg ccgccaacag ccccttgcgc cactcgtccg aggcctcgtc
      241 cggccaaccc atcaggtgcg cgagctccct tcccggtacc ggcttgacgt cccgttccag
      301 gatttcgacg aagcgccgcc cggccggcgc gtccaccgtt ccgctcccga cgacggagat
      361 gagcccgtgc acgtagccgc gccggtagtc ctcgtcctgt ttcggcggcg ccgcgaatcc
      421 gccgaagccg cgaagcgagt tgttcgtcgt caagtgcggg cggcagtccg gtcctcgacc
      481 ggcgcctgca acatgcttcc agcctcgctc ggtgagaaac cggtgatcgg cactcgcgac
      541 aagcgtcgtg ccatcttcca gcctgatgcg atacgcctcc ttcatggtcg accactggtc
      601 gcgcacctcg gtcaccacgt agcggcgata agcccccctg cgctcagtgc catacacgcg
      661 atcaccggat cgcacttcgg ctatcggttt gaggcgaccg tccgccatga gaattggggt
      721 gtcgccgccc acacagtagg cacaggcatg actgcatccc cggtacgggt tgatgctcca
      781 cttgaacggc atgccctgga cgcggttgag cgcggacttc gcctcgacct ccaggaaggt
      841 catgccggcg aactcgggcg tgtcgaacgt gcgcgtgcgc tccaccttga gcggcagcgt
      901 gggcgccgcc tcgggaaggg caacggacgg cccggggccc ccaccccggg ccgccatgcc
      961 ccgctgcagg tcggccaagg ttgagaaggc ggctgcagcg tcggaggtcg acatggcgcg
     1021 cagtatcgaa cacatgttcg aggcctgtca agcccctttt ccccgctcga gccggcgcac
     1081 ccgccgctcc acgccggccg ggaccacgcg ccgctcgcgc agcacccacg cccccctggt
     1141 cagcgcctca cccaggccgg cgcgcgcgtc ggcgtcgcgc agccccgcgc ccagcgcggc
     1201 cagcgtgtgc ccggccaccg ccgacagcgg gcggcgcagc cacgccgccc acagggcgtt
     1261 gcgccgctgg atgcgacggc gacgcgacgg gttgcggctg ggcgaggggt gatggtgcgc
     1321 gaccacgtcg tcgacgtagg ccaggcccca gccggccgtc gcgaggtcca gcgccagcag
     1381 ctcctcctcg ccgcccacca ggaagcgctc gtggaaaccg cccgcctgca ggaaggcgtc
     1441 acggcggacc accgcgccgc aggccacgaa gcccagcaca gacgggccgg gcaggtccgg
     1501 ctcggtgccg agcggcgagg ccgccatcgc gtcgcacacc gggtccaact cctcccgcgc
     1561 cccgaccagc atgcgcgccg cgagcagacc gaggcgcggg tgagcgtcga agtgctccgc
     1621 cgcccgggcc agggcgcccg gcgcccacca ggagtcgtcg tcgctgaacg ccaccagatc
     1681 cgtggcggcc tcgcgcgcgc cggcgttgcg ggcggccgca ccgatgttgc ggggcaggac
     1741 gacgacgcgc acgccgggaa agcgtgcccg caccatctgg gccgtgccat cggtcgaggc
     1801 gttgtcgacc acgatcaccg gcgacgccgg cgagagcccg gccagccgct ccagggcatc
     1861 ggccaggcca ccgcggcggt tctgcgtggc gaccacgacg gtcacgcgtt cggacatgcg
     1921 atctccatgg tggcgggcac gatgtccctt ccccgccggg ccggccccac acgcgctccg
     1981 gccgcgaaga cggccggtca ggaagccagc cggcgcaccg gccggcgctc caggcgccat
     2041 tcggggaagc cgccctccag gcg
//
LOCUS       DATMKC010000237         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_124166, whole genome shotgun sequence.
ACCESSION   DATMKC010000237 DATMKC010000000
VERSION     DATMKC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124166"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1046)
                     /locus_tag="VNU01_06930"
     CDS             complement(21..1046)
                     /locus_tag="VNU01_06930"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HWH32391.1"
                     /translation="MTVRFPEPLQPGDLIGVTSPSSGVPEALRPRLELCLEHLRAQGY
                     RARVGECMSGAGVVSAPAGERAAELTAMLLDPQVRAVVPPWGGELAVEVLPHLDLDAL
                     RQAEPTWVVGYSDLSTVLLPLTVTAGWATLHGHNLLDSPYRSPEPLAHWLDVVTLPRG
                     DSVTQGASRQHRAHGHDDWEDDPAVVERTWDSTGGWQPLDERPVRASGRLVGGCIETV
                     SVLAGTPYGDVRAFAEEHAPEGLVLHLEAAEEPALAIARHLWRMRLAGWFDHANAVLV
                     GRTTAPDSDGFTQRDAVRSVLGDLDVPVVLDVDCGHVPPHLSLVGGALAEVVVDGDVQ
                     QLRQLLA"
     gene            complement(1167..1418)
                     /locus_tag="VNU01_06935"
     CDS             complement(1167..1418)
                     /locus_tag="VNU01_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32392.1"
                     /translation="MDTPLLLVLLALPFLLAAVIVAVSSARRRRRTASTGSATEPRHR
                     GQADSATVQSAVLPARARLRKVTSGRDRRADSARSSARD"
     gene            complement(1713..2180)
                     /locus_tag="VNU01_06940"
     CDS             complement(1713..2180)
                     /locus_tag="VNU01_06940"
                     /inference="COORDINATES: protein motif:HMM:NF023691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HWH32393.1"
                     /translation="MKIEGYLFGFIALFLVPVTIVYWVLSGDPTGTACLAFSIGLAFM
                     VGYYLLFTARRMEARPEDRPDAEISEGSGEVGFFPPHSWWPIAMAGAFTVTCLGLIIG
                     PFLVLMGALGLAVTVMGFLFEYYVGMNRSQAQTLGALEAMGERPTSVHKFLGD"
     gene            complement(2182..>2891)
                     /locus_tag="VNU01_06945"
     CDS             complement(2182..>2891)
                     /locus_tag="VNU01_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWH32394.1"
                     /translation="VKFFNWIGTMWRGQLTFETPMLFSMGFLITFLLGGITGVLLASP
                     PIDFHVSDTYYVVAHFHYVLFGTVVFAFFGGVYYWFPKLTGRMMDDRLGKVHFWTLFV
                     GFHLTFMVQHWLGNEGMPRRYADYAAYEGFTTLNTISSIGAFVLGASTLPFLYNVWVS
                     FRRGQKAVGDDPWGFGNSLEWATSCPPPRHNFVTMPRIRSERPAFDLHYPQIAGNADF
                     HASPDDASMAPGKTASE"
BASE COUNT          433 a         1038 c         1069 g          351 t
ORIGIN      
        1 atcatgggat ccgcacgccg tcaggccagg agctgacgga gctgctgcac gtcgccgtcc
       61 acgaccacct ccgccagcgc accacctacg agggacaggt gcggcgggac gtgcccgcag
      121 tcgacgtcca gcacgaccgg cacgtccagg tcgcccagca cgctgcgcac cgcgtcccgc
      181 tgggtgaacc cgtcgctgtc cggcgccgtc gtacggccga cgagcacggc gttcgcgtgg
      241 tcgaaccagc cggccagccg catccgccac aggtgccggg cgatggccag cgcgggctcc
      301 tccgcggcct ccaggtgcag caccagcccc tccggggcgt gctcctcggc gaaggcccgc
      361 acgtcgccgt acggcgtgcc ggccaggacc gagacggtct cgatgcagcc gccgaccagg
      421 cggccgctcg cgcgcaccgg gcgctcgtcc aggggctgcc agccgccggt gctgtcccac
      481 gtccgctcga cgacggccgg gtcgtcctcc cagtcgtcgt gcccgtgcgc gcggtgctgc
      541 cgtgacgcgc cctgggtgac gctgtcgcca cgcggcaggg tcaccacgtc cagccagtgc
      601 gccaggggct cgggcgatcg gtacgggctg tccaggaggt tgtgcccgtg cagggtcgcc
      661 cagccggccg tcaccgtcag cggcagcagc acggtgctca ggtcgctgta gccgaccacc
      721 caggtcggct cggcctgccg cagcgcgtcg aggtccaggt gcggcagcac ctcgaccgcg
      781 agctcgccgc cccacggcgg gacgacggcg cgcacctgcg ggtcgagcag catcgcggtc
      841 agctcggcag cccgctcgcc agccggagcg ctgacgacgc cggcgccgct catgcactcc
      901 cccacccgcg cgcggtagcc ctgcgcgcgc aggtgctcga ggcagagctc cagccggggc
      961 cgcagcgcct ccggcacgcc gctggacggc gaggtgaccc cgatgaggtc accgggctgc
     1021 agcggctccg ggaagcggac ggtcacaccg gcagcctgtc agccgcgtcg accgcgttcc
     1081 gtgatgagag ccggctctgc cgacgcgggc ccacggcgcc ggatcctgcg tcgcggtcca
     1141 cggacggcga cgccgccgag ggtgggtcag tcgcgcgcgg acgaccgcgc cgagtcggcc
     1201 cggcggtcgc ggcccgaggt caccttgcgc aggcgcgccc gcgcggggag cacggcgctc
     1261 tggacggtgg cggagtcggc ctggccgcgg tggcgcggct cggtggcgga gccggtgctg
     1321 gcggtgcggc ggcgccggcg ggccgacgac acggcgacga tgactgcggc caggaggaag
     1381 ggcagggcca gcaggacgag cagcagcggg gtgtccatgg cgtcctctca gggcgggccg
     1441 gacgacccac cgtcacagcg ttcggcaggg acctccgtca ggttcggtac gcggtgccgc
     1501 ccggcggcgc gacgacggcg gccgccggag gcgccgctgc caccgggccg ctgcgggcct
     1561 cctgcctccc ggcagctccg gccatgaccc aggctcgccc gacccgcggc cgtggtcagg
     1621 gctgccggga cgccagcgct gggcgcatgg ctccgcgcaa cggcgacggc cggcccgcag
     1681 gtgcggaccg gccgtcgtac gagcgcgtgg gcctagtcgc ccaggaactt gtgcacgctg
     1741 gtcggccgct cccccatcgc ctcgagggcg ccgagcgtct gggcctgcga gcggttcatg
     1801 ccgacgtagt actcgaacag gaagcccatc acggtcacgg ccaggccgag ggcgcccatc
     1861 aggaccagga acgggccgat gatcagcccg aggcaggtca ccgtgaacgc gcccgccatc
     1921 gcgatcggcc accacgagtg cggcgggaag aagccgacct caccagagcc ctcgctgatc
     1981 tcggcgtccg gccggtcctc cggtcgcgcc tccatgcggc gggcggtgaa cagcaggtag
     2041 tagccgacca tgaaggccag gccgatggag aaggccaggc acgcggtgcc ggtcgggtcg
     2101 cccgagagca cccagtagac gatcgtgacc gggacgagga acagcgcgat gaagccgaac
     2161 aggtagccct cgatcttcat gtcactcgct cgccgtcttg ccgggcgcca tggacgcgtc
     2221 gtcgggtgag gcgtggaagt cggcgttccc cgcgatctgc gggtagtgca ggtcgaacgc
     2281 cgggcgctcc gagcggatgc gcggcatcgt cacgaagttg tgccgcggcg gcgggcagga
     2341 ggtcgcccac tccagcgagt tgccgaagcc ccacgggtcg tcccccacgg ccttctgccc
     2401 gcggcggaag ctgacccaca cgttgtagag gaacggcagc gtcgaggcgc cgagcacgaa
     2461 ggcgccgatg ctggagatcg tgttgagggt ggtgaagccc tcgtacgccg cgtagtcggc
     2521 gtagcgccgc ggcatgccct cgttgccgag ccagtgctgg accatgaacg tcaggtggaa
     2581 gccgacgaac agcgtccaga agtgcacctt gccgagccgg tcgtccatca tccggccggt
     2641 cagcttgggg aaccagtagt agacgccgcc gaagaaggcg aacacgaccg tgccgaacag
     2701 cacgtagtgg aagtgcgcca ccacgtagta ggtgtccgag acgtggaagt cgatcggcgg
     2761 gctggccagc agcacgccgg tgatcccgcc gagcaggaag gtgatgagga agcccatcga
     2821 gaacagcatc ggcgtctcga aggtcagctg ccctcgccac atcgtgccga tccagttgaa
     2881 gaacttcacg c
//
LOCUS       DATMKC010000238         3372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_26766, whole genome shotgun sequence.
ACCESSION   DATMKC010000238 DATMKC010000000
VERSION     DATMKC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26766"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VNU01_06950"
     CDS             complement(<1..296)
                     /locus_tag="VNU01_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32395.1"
                     /translation="MADSAAKRAPKGLPKRKLAALRKHLEAEQIQLRAQIAQLDADFA
                     DESWKARSDDDAESGTATFERERTMSLAQNARGLLSAIDRALDRMDDGTYGRCVS"
     gene            complement(308..643)
                     /locus_tag="VNU01_06955"
     CDS             complement(308..643)
                     /locus_tag="VNU01_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWH32396.1"
                     /translation="MSHDWSRAVRDRPGGARLRVRVVPRSSSPGFDGVAEDAPGGPAA
                     RLRLSSPPADGKANAEALRRVAAATGLAPRDVELVSGATSRVKTFSLSGLSASEAAGR
                     LAAAERGDG"
     gene            699..>3372
                     /gene="ileS"
                     /locus_tag="VNU01_06960"
     CDS             699..>3372
                     /gene="ileS"
                     /locus_tag="VNU01_06960"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWH32397.1"
                     /translation="MGFPEVDPQPDFPAIEQRVLDRWREQDVFARQLELRADGPLFRF
                     YEGPPTANGRPGVHHVESRAFKDIFPRYRAMKGYRVPRKAGWDCHGLPVELEIERELG
                     LARKQDIEDYGIEAFNARCRESVTRYVGDWERLTERIGFWVDLSDAYWTMSTDYVESV
                     WWSLKQLWDNDLIYQGHRVVPYCPRCGTALSDHEVALGYDTATDPSVYVRFPVLDGAL
                     AEEGADLLVWTTTPWTLISNTAVAVGEGIRYVLARDADGHRAVVVAADLVESALGEGA
                     QVLRDVPVDELVGLHYRGPFDYAQPDEPHGAWRTVLTGDFVSTDSGTGIVHLAPAFGE
                     DDMRVARAAGLPIVNPVDLEGRFDERVAPWTGQFVKDADPSIVEALREAGLLHRAGTY
                     EHTYPFCWRCKTPLLYYAKPSWYIRTTAIRDKLLAENAKVDWHPEHIRDGRYGDWLSN
                     NVDWSLSRERYWGTPLPLWRCEGCGDVEAIGSRAELGARAGRDLSELDPHRPYVDDVT
                     IPCGSCEGTKRRVPEVIDAWYDSGSMPFAQWGYPHKAGSEELFAERFPADYICEAIDQ
                     TRGWFYSLMAVSTLLFDANSYRTVVCLGHIVDAEGKKMSKSLGNILDPWEILDSRGAD
                     ALRWLLLTDGSPWVARRVGPEAVDEVIRKFLLTLWNTQNFFLTYARIEGWTPAASAAP
                     PVADRPVMDRWVLSELHRTVTTVDQALDAYDSTTAGRAIAAFVDDLSNWYVRRSRQRF
                     WRTRDDLDRADMEAAFSTLHECLVTVAALLAPFTPFVADHLWQALVTSVDEGAPDSVH
                     LADFPRPAPGLADERLAMAMRAARQITELGRRARNEARIGVRQPLARAAITLPEADRA
                     GWDLVAGVVAEELNVKALELASADASSSALTFSSS"
BASE COUNT          465 a         1233 c         1210 g          464 t
ORIGIN      
        1 gacacgcagc gtccgtacgt gccgtcgtcc atgcggtcca gcgcccggtc gatggccgac
       61 agcaggccgc gggcgttctg cgccagcgac atggtgcgct cccgctcgaa cgtggcggtg
      121 ccgctctcgg cgtcgtcgtc cgagcgggcc ttccaggact cgtccgcgaa gtccgcgtcg
      181 agctgtgcga tctgcgcgcg cagctggatc tgctcggcct ccagatgctt gcgcaaggca
      241 gccagcttgc gcttgggcaa gcccttcggg gccctcttgg cggcgctgtc tgccatggtg
      301 ggcgagtcta gccgtcgccc cgctcggcgg ccgcgaggcg ccccgccgcc tcggatgcgg
      361 acaatccgga caggctgaac gtcttgacgc gtgacgtggc gccggagacc agctcgacgt
      421 cgcgcggcgc caggcccgtc gcggcggcca cgcgccgcag cgcctccgcg ttcgctttgc
      481 cgtccgccgg cggggacgac aggcgcagcc gcgccgccgg gccgccgggc gcgtcctcgg
      541 ccacgccgtc gaagccgggc gacgacgagc ggggaaccac ccggacccgg aggcgcgccc
      601 cgcccggccg gtcccgcacc gcccggctcc agtcgtggct catgcgtgcg acgctaacat
      661 ccggcggaac acgacaggaa ccgaaaggac caccagacgt gggcttcccc gaggtcgacc
      721 cgcagccgga cttcccggcg atcgagcagc gcgtgctgga ccgctggcgc gaacaggacg
      781 tgttcgcccg ccagctcgag ctgcgcgccg acgggccgct gttccgcttc tacgaggggc
      841 cgcccaccgc caacggccgc ccgggcgtgc accacgtcga gtcgcgcgcc ttcaaggaca
      901 tcttcccgcg ctaccgcgcc atgaagggct accgggtgcc gcgcaaggcc ggctgggact
      961 gccacggcct gccggtggag ctggagatcg agcgggagct cggcctggcg cgcaagcagg
     1021 acatcgagga ctacggcatc gaggcgttca acgcgcgctg ccgcgagtcg gtcacccgct
     1081 acgtcgggga ctgggagcgc ctcaccgagc gcatcggctt ctgggtggac ctgtccgacg
     1141 cctactggac gatgtccacg gactacgtcg agagcgtgtg gtggtcgctc aagcagctgt
     1201 gggacaacga tctgatctac cagggccacc gcgtggtgcc ctactgcccg cgctgcggca
     1261 cggccctgtc cgaccacgag gtggcgcttg gctacgacac ggccaccgac ccgtccgtgt
     1321 acgtgcgctt cccggtgctg gacggcgcgc tggccgaaga gggcgcggac ctgctggtgt
     1381 ggaccaccac gccgtggacg ctgatctcca acacggccgt ggccgtcggc gagggcatcc
     1441 gctacgtgct ggcgcgcgac gccgacgggc accgggccgt ggtggtggcc gcggacctgg
     1501 tggaatcggc gctcggcgag ggcgcgcagg tcctgcgcga cgtgcccgtg gacgagctcg
     1561 tcggcctgca ctaccgcggc ccgttcgact acgcccagcc cgacgagccc cacggcgcgt
     1621 ggcgcaccgt gctgacgggc gacttcgtga gcaccgactc cggcaccggc atcgtccacc
     1681 tggcgccggc gttcggtgag gacgacatgc gcgtcgcgcg cgcggccgga ctgcccatcg
     1741 tgaaccccgt ggacctggag gggcggttcg acgagcgcgt ggcgccctgg acggggcagt
     1801 tcgtcaagga cgccgacccg tccatcgtcg aggcgctgcg cgaggcgggg ctgctgcacc
     1861 gcgccggcac ctacgagcac acgtatccgt tctgctggcg ctgcaagacc ccgctgctgt
     1921 attacgccaa gccgtcctgg tacatccgca ccacggccat ccgcgacaag ctcctggcgg
     1981 agaacgccaa ggtcgactgg caccccgagc acatccgcga cgggcgctac ggcgactggc
     2041 tgtccaacaa cgtggactgg tcgctttcgc gcgagcgcta ctggggcacg ccgctgccgc
     2101 tgtggcgctg cgagggctgc ggcgacgtgg aggcgatcgg ctcgcgggcg gagctcggtg
     2161 cgcgcgccgg gcgcgacctg agcgagctcg acccgcaccg gccgtacgtg gacgacgtca
     2221 cgatcccctg cggctcgtgc gaggggacca agcggcgcgt gccggaggtc atcgacgcct
     2281 ggtacgactc cggctcgatg ccgttcgcgc agtggggcta cccccacaag gccggcagcg
     2341 aggagctgtt cgccgaacgg ttcccggccg actacatctg cgaggcgatc gaccagaccc
     2401 gcggctggtt ctactcgctc atggcggtgt ccacgctgct gttcgacgcc aacagctacc
     2461 gcacggtcgt gtgcctgggc cacatcgtgg acgccgaggg caagaagatg tccaagtcgc
     2521 tgggcaacat cctggacccg tgggagatcc tggacagccg cggcgccgac gcgctgcgct
     2581 ggctgctgct caccgacggc tcgccgtggg tggcccgcag ggtgggcccg gaagccgtcg
     2641 acgaggtgat ccgcaagttc ctgctgaccc tgtggaacac ccagaacttc ttcctcacct
     2701 acgcccgcat cgagggctgg accccggcgg cctcggccgc gccgcccgtc gcggaccggc
     2761 ccgtcatgga ccgctgggtc ctaagcgagc tgcaccgcac cgtgaccacg gtggaccagg
     2821 cgctggacgc ctacgactcc accacggccg ggcgcgccat cgccgcgttc gtcgacgacc
     2881 tgtccaactg gtacgtgcgg cgcagccggc agcgcttctg gcgcacgcgc gacgacctgg
     2941 accgcgccga catggaagcg gcgttctcca cgctgcacga gtgcctggtg accgtggcgg
     3001 cgctgctcgc gccgttcacg ccgttcgtgg cggaccacct gtggcaggcg ctggtgacca
     3061 gcgtcgacga gggcgcccct gacagcgtgc acctggccga cttcccgcgg cccgcgccgg
     3121 gcctggccga cgagcggctg gccatggcca tgcgcgccgc gcgccagatc accgagctcg
     3181 gccgccgcgc ccgcaacgag gccaggatcg gggtgcgcca gccgctggcg cgcgccgcga
     3241 tcacgctgcc cgaggcggat cgcgcgggct gggacctggt cgccggcgtc gtggccgagg
     3301 agctgaacgt caaggcgctg gaactggcgt cggccgacgc cagttccagc gccttgacgt
     3361 tcagctcctc gg
//
LOCUS       DATMKC010000239         5988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_133050, whole genome shotgun sequence.
ACCESSION   DATMKC010000239 DATMKC010000000
VERSION     DATMKC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133050"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..717)
                     /locus_tag="VNU01_06965"
     CDS             complement(160..717)
                     /locus_tag="VNU01_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HWH32398.1"
                     /translation="MRQPALAERHLTVAVPDPLDIDPVVRASVEEATATAYERGFTDG
                     MAVGRREAYAEAEQLAEIIRGAARDAAVRLAEARAARSAEIVELALAVAEQVIGHEPH
                     DGGMVLLERVRSTLEQLDDEPLRVLVHPEDAPVVERGIVGARNVRVEADASLQPGEAR
                     VEGPWSRAELTFAGAMELVREVLDA"
     gene            complement(722..1735)
                     /gene="fliG"
                     /locus_tag="VNU01_06970"
     CDS             complement(722..1735)
                     /gene="fliG"
                     /locus_tag="VNU01_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HWH32399.1"
                     /translation="MSSVTQLSRAQKAAAVLVAIGPEAASKIMAFLTDAEQEQVALEV
                     ATLGQVKAEDAGQILREFHAEAVAHQFILKGGVDFARDLLRRTHGSEADGIIERLLAA
                     VSVSPFAFLRQRDPAQLVQYLQEEHPQTVALVLAHLPPGNAARVIAGLPEHVQREVAF
                     RLATMESTSPEVIAQVETAMQTRFGSVRGREELSKKGGVKDLASILNSSDRQTERLIL
                     AGLEATDPDIAEEVRALMFVFEDIVLLDDRAVQEVLREIDASTLALAMKGVTDTVSDK
                     ILSNVSQRAAEALREELEVLGAVRVRDVEAAQSEIVRHIRRLEEDGRIIVKRGGEGGD
                     VIE"
     gene            complement(1732..3261)
                     /gene="fliF"
                     /locus_tag="VNU01_06975"
     CDS             complement(1732..3261)
                     /gene="fliF"
                     /locus_tag="VNU01_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HWH32400.1"
                     /translation="MELRDRFSEFIRALPLAHRVVIVAAAAGLLMGGLMFARWVTAPS
                     WTVLYSGMDDGTIAEAIDELESQGVPYQLEGGGSTILVPRERLYTTRARLAQAGLSGR
                     PTPKGYEILDSQGLSVSDFKQRVDYQRALEGELSKTLGAMEPVKAATVHLVMPDKQLF
                     VEREEQLPVTASVLLQTRRDLSVDEIDTVTFLVSSAVEGLEPDEVTVADADGTVLHAP
                     GDTSGPAGITNRQLRHTREFESALAADLSSLLARVTNDDRAAVVVRASLNYDERTIEQ
                     ETFGPGDGVMRSEATENETFAGTDGGVAPGGLVGVDGGPVNVTQGETDYDREQASREF
                     AVDRLLERTDAAPGGIEKLSVAIVMDDGSLTGVDVPPVAEVEELVSAALGLDPARGDT
                     LAVSTVPFPTLDEAEEAGTGLTGFLTDLLPRAIAGLVLLLVSVALFLMSRGRRRTVAD
                     QPMWGRVELGAGGEPEMVEVGSLEGATPEANVTQDLHELVQRQPEEIASLLRGWLADR
                     R"
     gene            complement(3265..3573)
                     /gene="fliE"
                     /locus_tag="VNU01_06980"
     CDS             complement(3265..3573)
                     /gene="fliE"
                     /locus_tag="VNU01_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HWH32401.1"
                     /translation="MAVPGIGAVGPGVFGGLPNVGGAPRVPGGGQDFGQMVKAGLQNV
                     SNAEAAVDQVAQTIASGGPAEIHDLMAASAKAQVQVDLLVQVRNRAVEAYQEIMRIQV
                     "
     gene            complement(3573..3980)
                     /locus_tag="VNU01_06985"
     CDS             complement(3573..3980)
                     /locus_tag="VNU01_06985"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HWH32402.1"
                     /translation="MTFSIIDIGRTGAGFSRYWLDNVAHNLANVNTVRPGDEEPFRAR
                     VVEARALTSQDGTGAFGVGVADVHDQAGDPMRVYDPGNPLADEGGYVNMPVVDMPAEM
                     VNLIQAQRSYQLSMKVIQSGHEAYQSALRLGNR"
     gene            complement(3977..4336)
                     /locus_tag="VNU01_06990"
     CDS             complement(3977..4336)
                     /locus_tag="VNU01_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32403.1"
                     /translation="MLPISDSTMRAVEYALDGLKTRADTRAHNIANVNTPGFAAKKVD
                     FESSLRAALAFGSPERAPAPAVVPGDGLPNKQGNTVDLETELVGFMKDNLMRDAMVNA
                     YNFKVGVLRTAINGMPR"
     gene            complement(4514..4825)
                     /locus_tag="VNU01_06995"
     CDS             complement(4514..4825)
                     /locus_tag="VNU01_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32404.1"
                     /translation="MTASVIDWTAALDEWESFLDELEGALDSGEWEAHAERPPWAPPG
                     DVIGELGPEGEARALALADRAGRLRERLDRALRATAAEIGEGRRKGAAVSAYARQQRR
                     R"
     gene            complement(4822..5208)
                     /gene="fliS"
                     /locus_tag="VNU01_07000"
     CDS             complement(4822..5208)
                     /gene="fliS"
                     /locus_tag="VNU01_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HWH32405.1"
                     /translation="MSGNPYQQQAVTTANPAQLVLMLYDGVLTAIARARAAEPLGVEG
                     LPVVNRELQRAQDILTELMVTLDRERGGTVAESLARLYDFSLDRLIRANVSKDLSLLD
                     PVVEVVRSLRDAWEAACCNPPVAATG"
     gene            complement(5205..>5988)
                     /gene="fliD"
                     /locus_tag="VNU01_07005"
     CDS             complement(5205..>5988)
                     /gene="fliD"
                     /locus_tag="VNU01_07005"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HWH32406.1"
                     /translation="TYINATDDTQPGTYGVTVTTAAQTAAVTGAAYAAEAQTLTITSG
                     GLSADTVLDGTETIAQAITKINDALAAEGITTITATDDGGAIRLADNRYGSAVTFDVT
                     STGGGFGLTGTHTGVDVVATVDGTSYTGKGRTLTIDDDGKDVDGLTVRITATAADVAG
                     AGGALSLGNLVYTTGLAGRVSSRVSGYEGSAGSIQVLRDGIEQQIDDFQSRIDAMEER
                     LKVKEAALVRQFSAMERMMGQLASQGNWLAAQLSAMAAGTRR"
BASE COUNT          794 a         2239 c         2052 g          903 t
ORIGIN      
        1 agcgcgacca cctcggccgg caggtcgccc tgggcggcgt gcacgacgag cgcgtcgccg
       61 atgcgcagcc gcaggccgcg gatctcgatc tcatggccga ccacgcgcgc caccgccccg
      121 gcaacccgcc agggccggac ctcgcgcacc gccgcgacgc taggcatcga gcacctcgcg
      181 caccagctcc atggcgccgg cgaaggtcag ctccgcccgt gaccacggcc cctcgacccg
      241 ggcctcgccc ggctggagcg acgcgtcggc ctccacgcgg acgttgcggg cgccgacgat
      301 gccgcgctcg accacgggcg cgtcctccgg gtgcaccaga acgcgcagcg gctcgtcgtc
      361 gagctgctcc agcgtcgagc gcacgcgctc cagcagcacc atccccccgt cgtggggctc
      421 atggccgatg acctgctcgg cgaccgccag cgccagctcc acgatctcgg cggaccgcgc
      481 ggcgcgcgcc tccgccaggc gcacggcggc gtcgcgcgcg gcgccgcgga tgatctcggc
      541 gagctgctcg gcctcggcgt aggcctcgcg gcgaccgacc gccatgccgt cggtgaagcc
      601 gcgctcgtag gcggtggcgg tcgcctcctc cacgctggcg cgcacgaccg ggtcgatgtc
      661 cagcgggtcg ggaaccgcca cggtgaggtg acgctcggcg agcgcgggct ggcgcatgac
      721 cctactcaat gacgtcaccg ccctcgccgc cgcgcttcac gatgatccgg ccgtcctcct
      781 ccaggcggcg gatgtgccgc acgatctcgc tctgcgccgc ctcgacgtcg cggacgcgca
      841 cggcgccgag cacctcgagc tcctcgcgca gggcctcggc ggcgcgctgg gacacgttgc
      901 tgaggatctt gtcgctgacc gtgtccgtga cgcccttcat ggccagcgcc agcgtggagg
      961 cgtcgatctc ccgcagcacc tcctgcacgg cgcggtcgtc cagcagcacg atgtcctcga
     1021 agacgaacat cagcgcgcgc acttcctcgg cgatgtccgg gtccgtcgcc tccagccccg
     1081 ccaggatcag gcgctccgtc tggcgatccg aggagttcag gatcgacgcg aggtccttca
     1141 ccccgccctt cttggacagc tcctcgcggc cgcgcaccga gccgaagcgc gtctgcatcg
     1201 ccgtctcgac ctgcgcgatg acctccggcg acgtcgattc catcgtcgcc aggcggaacg
     1261 ccacctcacg ctgcacgtgc tccggcagcc cggcgatcac ccgggcggcg ttgcccggcg
     1321 gcaggtgggc gagcaccagc gcgacggtct gcgggtgctc ctcctggagg tactgcacga
     1381 gctgcgccgg gtctcgctgg cgcaggaacg cgaagggcga cacggacacc gccgcgagca
     1441 ggcgctcgat gatcccgtcg gcctcggagc cgtgcgtgcg ccgcagcagg tcgcgcgcga
     1501 agtccacgcc tcccttgagg atgaactggt gggcaacggc ctcggcgtgg aactccctga
     1561 ggatctgccc ggcgtcctcg gctttgacct ggccgagcgt ggcgacctcc agggccacct
     1621 gctcctgctc ggcgtccgtc aggaacgcca tgatcttgga cgccgcctcc ggaccgatcg
     1681 cgaccaggac cgcggcggcc ttctgggcgc gggacagctg cgtgacgctg ctcatcggcg
     1741 atcagccagc cagccgcgca agagcgaggc gatctcctcc ggctggcgct gcaccagctc
     1801 gtgcaggtcc tgggtgacgt tggcctccgg cgtcgcgcct tccagcgagc ccacttccac
     1861 catctcgggc tcaccgccgg cgcccagctc cacgcggccc cacatcggct ggtccgcgac
     1921 cgtgcgccgg cgtccgcggc tcatcaggaa cagcgccacg ctgacgagca gcaggacgag
     1981 tccggcgatg gcccgcggca ggagatccgt caggaagccg gtgaggccgg tgccggcctc
     2041 ctcggcctcg tccagcgtgg ggaacggcac ggtggacacc gcgagcgtgt cgcccctggc
     2101 cggatccagg cccagcgccg cgctgaccag ctcctcgacc tccgccaccg gcggcacgtc
     2161 cacaccggtg agcgagccgt cgtccatcac gatcgcgacc gagagcttct cgatgccgcc
     2221 gggggcggcg tcggtgcgct cgagcaagcg gtccacagcg aactcccgcg aggcctgctc
     2281 gcggtcgtag tccgtctcgc cctgcgtcac gttgaccggc ccgccgtcca cgcccacgag
     2341 tccgcccggc gcgacaccgc cgtcggtgcc cgcgaaggtc tcgttctccg tggcctcgct
     2401 gcgcatgacg ccgtcaccgg ggccgaaggt ctcctgctcg atcgtgcgtt cgtcgtagtt
     2461 gaggctcgcg cgcacgacca cggcggcccg gtcgtcgttc gtgacgcgcg ccagcaggga
     2521 cgacaggtcc gccgcgagcg cggactcgaa ctcgcgggtg tgccggagct ggcggttggt
     2581 gatcccggcc ggcccgctgg tgtcgccggg ggcgtgcagc acggtgccgt ccgcgtccgc
     2641 gaccgtcacc tcgtcgggct cgagcccctc cacggcggag gacaccagga aggtcacggt
     2701 gtcgatctcg tcgacgctca ggtcccggcg cgtctggagc aaaaccgagg cggtgacggg
     2761 aagctgctcc tcacgctcca cgaagagctg cttgtccggc atgaccaggt gcacggtggc
     2821 cgccttgacg ggctccatcg cccccagcgt cttggacagc tcgccctcca gcgcgcgctg
     2881 gtagtccacg cgctgcttga agtcggagac cgacagcccc tgcgagtcca ggatctcgta
     2941 gcccttcggc gtcggccggc ccgacaggcc cgcctgggca aggcgggcgc gcgtggtgta
     3001 gaggcgctcg cgcggcacca ggatcgtcga cccgccgcct tcgagctggt acggcacgcc
     3061 ctggctctcg agctcgtcga tggcctcggc gatcgtgccg tcgtccatcc cggagtacag
     3121 caccgtccac gacggcgcgg tcacccagcg cgcgaacatg aggccgccca tgagcaggcc
     3181 cgccgccgcg gcgacgatga ccacccggtg cgccaggggc agcgcccgga tgaactcgga
     3241 gaatcggtcc cgcagctcca tccgctacac ctggatcctc atgatctcct ggtaggcctc
     3301 caccgcgcga ttgcgcacct gcacgaggag gtcgacctgg acctgcgcct tcgcgctggc
     3361 cgccatcaga tcgtggatct ccgccggccc accggacgcg atcgtctgcg ccacctggtc
     3421 cacggccgcc tcggcgttgg agacgttctg cagcccggcc ttgaccatct ggccgaagtc
     3481 ctgcccgccg ccgggcacgc gtggcgcccc gccgacgttc ggcagcccgc cgaagacgcc
     3541 cgggccgacg gcgccgatgc cggggaccgc catcagcggt tccccagccg gagggcgctc
     3601 tgatacgcct cgtggccgct ctggatgacc ttcatcgaaa gctggtagct gcgctgcgcc
     3661 tggatgaggt tgaccatctc cgcgggcatg tccaccaccg gcatgttgac gtagccgccc
     3721 tcgtcggcga gggggttccc ggggtcgtag acccgcatgg gatcgcccgc ctgatcgtgc
     3781 acatcggcca cgccgacgcc gaacgcgccg gtgccgtcct gcgaggtgag cgcgcgcgcc
     3841 tccacgaccc gggcgcggaa cggctcctcg tcgccggggc gcaccgtgtt cacgttcgcg
     3901 aggttgtggg cgacgttgtc cagccagtag cgggagaagc ccgccccggt gcggccgatg
     3961 tcgatgatgg agaacgtcat cgcggcatcc cgttgatcgc cgtgcgcagc acgcccacct
     4021 tgaagttgta ggcgttgacc atcgcgtcgc gcatgaggtt gtccttcatg aagccgacga
     4081 gctcggtctc caggtccacc gtgttgccct gcttgttggg cagcccgtca ccgggcacca
     4141 cggcgggcgc gggggcgcgc tcgggggagc cgaaggccag cgccgcgcgc agcgacgact
     4201 cgaagtcgac cttcttggcc gcgaagccgg gcgtgttcac gttggcgatg ttgtgcgccc
     4261 gcgtgtcggc gcgcgtcttc aacccgtcga gtgcgtactc gacggcccgc atcgtgctgt
     4321 cgctgatcgg caacatgcgc cactctccca cagccgcggg accgccaggg aaaacagcaa
     4381 agggaagacg caagcgtctt ccctgaccct ttggcgatcc aactcggttg tcctgcacga
     4441 tgaccggtcg gccgcaggag agcagaagtt aagggaatcc cgagatcggt cgtcctcggc
     4501 gtgcgaagcc cggtcagcgc ctccgctgct ggcgggcgta cgcgctcacg gcggcgccct
     4561 tgcggcggcc ctcgccgatc tccgcggccg tcgcgcggag cgccctgtcg agtcgttcgc
     4621 gcagccggcc ggcccggtcg gccagtgcga gggcgcgcgc ctctccctcg ggccccagct
     4681 caccaatgac gtccccgggc ggcgcccatg gcggacgctc cgcgtgcgcc tcccactcac
     4741 cgctgtccag ggcgccctcc agttcgtcca ggaaggactc ccattcgtcg agcgcggcgg
     4801 tccagtcgat cacggaggcg gtcatcccgt cgccgccacg ggcgggttgc agcaggccgc
     4861 ctcccaggcg tctcgaaggc tgcggacgac ctccacgacc gggtccagca gcgacaggtc
     4921 cttgctcacg ttcgcgcgga tcagccggtc gagtgagaag tcgtacaacc gtgccaggct
     4981 ctccgcgacg gtgcccccgc gctcgcggtc cagcgtgacc atcagttcgg tgaggatgtc
     5041 ctgcgcgcgc tgcagttccc ggttcacgac cgggagccct tccacaccga gcggctcggc
     5101 ggcgcgcgcg cgggcgatgg cggtcaacac cccgtcgtag agcatcagga cgagctgtgc
     5161 gggattggcc gtcgtcaccg cctgctgctg gtaggggttc ccgctcatcg ccgcgtcccc
     5221 gccgccatgg cgctgagctg tgccgccagc cagttcccct gcgatgccag ctgccccatc
     5281 atccgctcca tcgcggagaa ctggcgcacc agcgcggctt ccttgacctt cagccgctcc
     5341 tccatggcgt cgatgcggct ctggaagtcg tcgatctgct gctcgatgcc gtcgcgcagc
     5401 acctggatgc tgccggcgga gccctcatag ccagagaccc ggctcgacac gcggccggca
     5461 aggccggtcg tgtagaccag gttgccgagg ctgagcgccc cgcccgcgcc cgccacgtcg
     5521 gccgccgtgg cggtgatgcg caccgtcagc ccgtcgacgt ccttgccgtc gtcgtcgatc
     5581 gtgagcgtgc gccccttgcc ggtgtaggac gtgccgtcga ccgttgccac gacgtccacg
     5641 cccgtgtggg tgccggtgag accgaaccct ccgcctgtgc tggtgacgtc gaaggtcacg
     5701 gccgacccat agcggttgtc ggccagacgg atggctccgc cgtcatcggt cgccgtgatg
     5761 gtggtgatgc cttccgccgc gagcgcgtcg ttgatcttgg tgatcgcctg ggcgatggtc
     5821 tcggtgccgt ccaggacggt gtcggcgctc agcccaccgc tggtgatggt cagcgtctgg
     5881 gcctcggccg cataggcggc gcccgtgacg gccgccgtct gggccgcggt cgtcacagtg
     5941 acaccgtagg tgccgggctg ggtgtcgtcc gtggcgttga tgtaggtg
//
LOCUS       DATMKC010000240         3560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_314404, whole genome shotgun sequence.
ACCESSION   DATMKC010000240 DATMKC010000000
VERSION     DATMKC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314404"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..752
                     /locus_tag="VNU01_07010"
     CDS             537..752
                     /locus_tag="VNU01_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32407.1"
                     /translation="MAPPPQNVAADALLGLLLPLWQIAIAVCVVVVLIGSVRRLARRG
                     PSRMTTALVVTGGAVLALAMIGWLLSG"
     gene            complement(819..1616)
                     /locus_tag="VNU01_07015"
     CDS             complement(819..1616)
                     /locus_tag="VNU01_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWH32408.1"
                     /translation="MRVAVCQLNARGDRLANLRQARELLGRAAAGGADVAVLPEYVDY
                     LGPEAEAPRPEPVDGEFAAYFADAARALRMWVVAGSFHETGPDPAHTYNTSLVFDRGG
                     ALAAAYRKIHLYDVEIPGRVSYRESRSVAPGEAPVVVDVEGVGFGLSVCYDLRFPELY
                     RRLAVDGGARVLVVPAAFMLHTGRDHWEVLLRARAIENQCYVLASAQLGDHDPGRTCF
                     GRSMVVDPWGTVVAQAPDVVGVTFADLDLARLDRIRAELPSLANRRM"
     gene            complement(1636..2916)
                     /gene="clpX"
                     /locus_tag="VNU01_07020"
     CDS             complement(1636..2916)
                     /gene="clpX"
                     /locus_tag="VNU01_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWH32409.1"
                     /translation="MARPTDSNEQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEELTAPATFDLENLPKPREIYSVLQEYVVGQEPAKRTLSVAVYNHYKRVQMMQAE
                     EQDLELQKSNILLLGPTGCGKTLLAQTLAKILNVPFAIADVAGLGEGRGVGEDVENIL
                     LKLIQAADYDIKRAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTVA
                     NVPPQGGRKHPHQEFIQIDTTNVLFICGGAFAGLDRIIEARTGKGGVGFGAHLRAVSE
                     RSTDDVFAQVMPEDMLKFGLIPEFIGRLPVITSVRSLDRKALVSILTEPRNALVKQYQ
                     RLFELDGVELEFEPDALEAIADQAMLRGTGARGLRAILEEVLLSVMYEVPSNPDAARV
                     LITRAVVLENVNPTIVPREFGSRRPRRERGEKSA"
     gene            complement(2926..3510)
                     /gene="clpP"
                     /locus_tag="VNU01_07025"
     CDS             complement(2926..3510)
                     /gene="clpP"
                     /locus_tag="VNU01_07025"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWH32410.1"
                     /translation="MSPLVPMVVEQTSRGERAFDIYSRLLNERIVFLGTPVTDEIANL
                     IVAQLLHLESEDPDKDISLYVNSPGGSVYAGLAIYDTMQFIKPDVQTICVGIAMSMGA
                     LLLSGGAEGKRMALPNAKILIHQVSSSFQGQATDIEIHAKEIIDVRKRLDEILALHTG
                     QELEKVARDTERDYFMSAEEAVEYNLIDRVIAHH"
BASE COUNT          539 a         1218 c         1219 g          584 t
ORIGIN      
        1 aaaaaattag ccaaacccaa ccaccaaagc ggccaggtca acaaaaacta aagcactggc
       61 aaaaaagcac cctgttgagt tctcaaagaa caaacacaca ccacaaacaa cccaccacac
      121 cggtggacca tccgtagggc aactccacta ctttagccat ccagccccgg accgtcaagc
      181 cataaggccg tggtccgtgt cactggaagg atcccatacc tagctccgcc gcgtacggcg
      241 aatcggggga agccgtagac cggccgacgg tcatttctga ctcatccgcc cggctcctgc
      301 cggcttgacc attctacccg gtcggctccg cgatcgcaaa cccggcttcc cggtcgcacc
      361 gcaccacccg gaccagcaac ccggggaccg ggtctctggg gtccgcctgg gcgctccgac
      421 cgttttgtcc ggtgcgccct ggcagagaga aagttacgcg ctacggtctg cggagtcaaa
      481 tcgcctggtc agacgcgtgc agcgcgccca cgcccgggcc gtgtcacagt gggcggatgg
      541 ctcccccgcc ccagaacgtc gccgcggacg cgctgctcgg tctgctgttg ccgctgtggc
      601 agatcgcgat cgcggtctgc gtggtcgtcg tgctgatcgg gtcggtgcga cggctggccc
      661 ggcggggccc ctcgcggatg accacggcgc tggtcgtcac cggcggcgcc gtgctggccc
      721 tggcgatgat cggctggctg ctgtcgggct gaccgccggg ggcgaccgcg gcggtcagcc
      781 ggtgccgtac gtcgccgcgg cggcccgcgg cgcgggcgtc acatgcggcg gttggccagg
      841 ctcggcagct cggcgcggat gcggtcgagc cgcgccaggt ccaggtcggc gaaggtcacg
      901 ccgaccacgt ccggcgcctg cgccaccacg gtgccccacg ggtcgaccac catgctgcgg
      961 ccgaagcagg tccggcccgg gtcgtgatcg ccgagctggg cgctggcgag gacgtagcac
     1021 tggttctcga tggcccgggc gcgcagcagg acctcccagt ggtcgcgccc ggtgtgcagc
     1081 atgaacgccg ccggcaccac cagcacccgc gccccgccgt cgacggccag acgccggtag
     1141 agctccggga agcgcaggtc gtagcagacc gacaggccga acccgacgcc ctcgacgtcg
     1201 accaccaccg gcgcctctcc gggggccacg ctgcgcgact cgcggtagga cacccggccc
     1261 gggatctcga cgtcgtacag gtggatcttg cggtacgccg cggcgagcgc gccgccgcgg
     1321 tcgaacacca gcgaggtgtt gtacgtgtgg gcggggtcgg gccccgtctc gtggaacgac
     1381 ccggccacca cccacatccg cagcgcgcgc gccgcgtcgg cgaagtaggc cgcgaactca
     1441 ccgtcgaccg gctcgggcct gggcgcctcg gcctcggggc cgaggtagtc cacgtactcc
     1501 ggcagcacgg cgacgtcggc gccccccgcc gcagcccggc ccagcagttc ccgcgcctgg
     1561 cgcaggttcg ccaaccggtc gccgcgggcg ttgagctggc agacggctac gcgcatgcgg
     1621 ccagcgtagc cgcggtcagg ccgacttctc cccgcgctcg cggcgcggcc ggcggctgcc
     1681 gaactcccgc ggcacgatgg tggggttcac gttctccagc acgaccgccc gggtgatcag
     1741 gacccgtgcg gcgtccgggt tgctcggcac ctcgtacatg acgctgagca ggacctcttc
     1801 gaggatcgcc cgcagcccgc gggcgccggt gccgcgcagc atggcctggt cggcgatcgc
     1861 ctccagcgcg tcgggctcga actccagttc cacgccgtcc agctcgaaga ggcgttggta
     1921 ctgcttgacc agcgcgttgc gcggctcggt caggatgctg accagtgcct tgcggtccag
     1981 gctgcgtacg ctggtgatca ccggaagccg gccgatgaac tccggaatca gcccgaactt
     2041 gagcatgtcc tcgggcatga cctgggcgaa cacgtcgtcc gtcgagcgct ccgagaccgc
     2101 gcgcaggtgg gcgccgaagc cgacgccgcc cttgccggtg cgggcctcga tgatccggtc
     2161 cagcccggcg aaggccccgc cgcagatgaa caacacgttg gtggtgtcga tctggatgaa
     2221 ctcctggtgg gggtgcttgc ggccgccctg gggcggtacg ttcgccaccg tcccctcaag
     2281 aatcttgagc agcgcctgct gcacgccctc ccccgacacg tcgcgggtga tcgacgggtt
     2341 ttccgacttg cgagcgatct tgtcgacctc gtcgatgtag atgatgccgg tttccgcgcg
     2401 tttgatgtcg tagtcggcgg cctggatgag cttgagcagg atgttctcca cgtcctcgcc
     2461 gacgccacgg ccctcaccga ggccggctac gtcggcgatg gcgaacggga cgttgaggat
     2521 cttcgcgagc gtctgcgcga gcagcgtctt gccgcagccc gtggggccca gcagcaggat
     2581 gttggacttc tgcagctcga ggtcctgctc ctcggcctgc atcatctgca cgcgcttgta
     2641 gtggttgtag accgcgacgc tgagcgtgcg cttggccggc tcctggccga cgacgtactc
     2701 ctggaggacc gagtagatct cccgcggctt cggcaggttc tcgagatcga acgtggcggg
     2761 agcggtgagc tcctcgtcga tgatctcgtt gcagagatcg atgcactcgt cgcagatgta
     2821 gacacccgga cccgcgatga gcttcttgac ctgccgctgg gacttcccgc agaagctgca
     2881 gaggagctgc tcgttggagt ccgttggccg agccatcaca cgagcctagt ggtgcgcgat
     2941 gaccctgtcg atgaggttgt actccactgc ctcttcggcg ctcatgaagt agtcgcgctc
     3001 ggtgtcgcgc gcgaccttct cgagctcctg cccggtgtgc agggcgagga tctcgtcgag
     3061 gcgcttgcgc acgtcgatga tctccttcgc gtggatctcg atgtcggtgg cctggccctg
     3121 gaaggacgag gagacctggt ggatgaggat cttggcgttc ggtagcgcca tgcgcttgcc
     3181 ctccgcgccg cccgagagca ggagcgcgcc catggacatc gcgatgccga cgcagatggt
     3241 ctgcacgtcg ggcttgatga actgcatcgt gtcgtagatc gcgaggcccg cgtagaccga
     3301 gccgcccggc gagttgacgt agagcgagat gtccttgtcg gggtcctccg actcgaggtg
     3361 gaggagctgc gcgacgatca ggttggcgat ctcgtccgtg accggcgtcc cgaggaacac
     3421 gatccgctcg ttgaggaggc gggagtagat gtcgaacgcg cgctccccgc gggaggtctg
     3481 ctcgacgacc atcgggacaa gtgggctcat ggtcctcgct ttgtttcgct tggcgggcgg
     3541 gaatcctccc cgggctgcgg
//
LOCUS       DATMKC010000241         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_50013, whole genome shotgun sequence.
ACCESSION   DATMKC010000241 DATMKC010000000
VERSION     DATMKC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50013"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VNU01_07030"
     CDS             <1..578
                     /locus_tag="VNU01_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWH32411.1"
                     /translation="SLAGGGGANAESLLFGSILGVRGVDAVAALAVAAAVALVLVVGW
                     KRLLFTAFDPVVARVQGVRTRAVQLALDLLVAGVVVAGVRLLGVLMVAAAVVIPPAAA
                     RLVSHRAGTVVLLGGAIGAASQVAGLFVSWHWAVPSGPAIVLIAVNLFLLAALATSIQ
                     RLTRLARARRSAGPAGGTQDARQGKGSAASP"
     gene            614..2329
                     /locus_tag="VNU01_07035"
     CDS             614..2329
                     /locus_tag="VNU01_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32412.1"
                     /translation="MRKPLLRLYGPFIALALAQALFIAVAPSTSPGQDVAFEPGDYAA
                     PDDVGEELAAGDVPGGEPAVGDGIEAGTPAQQSGGTAGTPGTTGGGQSPAGRQDGSAT
                     PVGDTSHCVDGRQHGKYYQAPPCVPKWSGDNGGATAPGVTGDTIKIYFYREKKNEVVA
                     AILQQEGLHRSDAEEEDFAEAMQDFLNAHYEFYGRKIDIQVRYGDCPETPPDVPLCKA
                     EAREIAKERPFMVLWPVPLYPDIFDEFYKLGVIAIGGWHFDERYFTQRRPYRYDVFMD
                     GTRTADIAAEYYCKKMARRNASHSGPVIHPTIGARDQVPRRMGIITPENPAFLEAAKR
                     LQQLVTACDAQAPVLVSYESNIERSQTQANANISALIDGKVTTVVCMCDPIAPVFRTQ
                     TATRQGYFPEHLLSGSGLLDYDKLGRLYEPSQWAHAFGPSHLQTSPVFEQTEAAVVWR
                     ESGRPGNPCLACNLPWAYYNLAAALIQGAGPNLNAATVARAADEFPPTGGWQQTGGRP
                     EVVLSDMGPGDYTAVSDIKEVYWSPTAPSNIDRRAGAYVPLRGGRRYRAGELDSTFEI
                     PVPTD"
BASE COUNT          334 a          841 c          853 g          315 t
ORIGIN      
        1 tcagcctggc gggcggcggg ggcgcgaacg cggagagcct gctgttcggc agcatccttg
       61 gcgtgcgcgg ggtcgacgcc gtggcggcgc tggcggtcgc cgcggccgtg gcgctggtgc
      121 tcgtggtggg gtggaagcgg ctgctgttca ccgccttcga ccccgtggtg gcgcgcgtcc
      181 agggcgtgcg cacgcgcgcg gtgcagctcg ccctggacct gctggtggcg ggcgtggtcg
      241 tcgcgggtgt gcgcctgctc ggcgtgctca tggtggccgc cgccgtcgtc atccctccgg
      301 cggcggcccg cctggtctcc caccgcgcgg gcacggtcgt gctgctgggc ggggcgatcg
      361 gcgccgcgag ccaggtcgcc ggactgttcg tgtcctggca ctgggcggtg ccgagcgggc
      421 cggccatcgt gctgattgcc gtgaacctat tccttttggc cgctttggcc actagcatcc
      481 agcgcctgac caggctggcg cgcgccaggc ggtcggcggg acctgccggt gggacgcagg
      541 acgcgcgtca agggaagggc tcggcggcgt cgccatgacc gccaggaccg taggagcgaa
      601 cggagcggaa cgcatgcgca agccccttct gcggctgtac ggccccttca tcgcgttggc
      661 gctcgcgcag gcgctgttca tcgcggtcgc cccgtcgacg tcgcccggcc aggacgtggc
      721 gttcgagccc ggtgactacg cggcgcccga cgatgtcggc gaggagctcg cggccggcga
      781 cgtgccgggc ggcgagcccg ctgtcggcga cggcatcgag gcggggactc cggcgcagca
      841 gtccggcggc acggcgggca ccccgggcac caccggcggg ggccagtcgc ccgccggccg
      901 gcaggacggc tccgcgaccc ccgtcggcga caccagccac tgcgtggacg gccggcagca
      961 cggcaagtac taccaggcgc cgccgtgcgt gccgaagtgg agcggcgaca acggcggggc
     1021 caccgcgccc ggcgtgaccg gcgacacgat caagatctac ttctaccggg agaagaagaa
     1081 cgaggtcgtc gcggcgatcc tccagcagga gggtctgcac cgcagcgacg ccgaggaaga
     1141 ggacttcgcc gaggcgatgc aggacttcct caacgcccac tacgagttct acgggcgcaa
     1201 gatcgacatc caggtccgct acggcgactg cccggagacc ccgccggacg tgccgctgtg
     1261 caaggccgag gcgcgcgaga tcgccaagga acggcccttc atggtcctct ggcccgtgcc
     1321 gctgtacccg gacatcttcg acgagttcta caagctgggc gtcatcgcga tcggcgggtg
     1381 gcacttcgac gagcgctact tcacgcagcg ccgcccgtac cgctacgacg tgttcatgga
     1441 cggcacccgc acggccgaca tcgccgccga gtactactgc aagaagatgg cccgccgcaa
     1501 cgcctctcac agcgggccgg tgatccaccc gaccatcggc gctcgcgacc aggtgccgcg
     1561 gcgcatgggc atcatcacgc cggagaaccc ggcgttcctg gaagcggcca agcgcctgca
     1621 gcagctggtc accgcctgcg atgcgcaggc gcccgtgctg gtgagctacg agtccaacat
     1681 cgagcgcagc cagacgcagg ccaacgccaa catctccgcg ctgatcgacg gcaaggtcac
     1741 cacggtcgtg tgcatgtgcg acccgatcgc gccggtgttc cgcacgcaga ccgcgacgcg
     1801 ccagggctac ttccccgagc acctgctgag cggctcgggt ctgctggact acgacaagct
     1861 cggacggctg tacgagccga gccaatgggc gcacgcgttc ggccccagcc atctgcagac
     1921 gtccccggtg ttcgagcaga ccgaggcggc ggtggtctgg cgtgagagcg gccgccccgg
     1981 caacccctgc ctggcctgca acctgccgtg ggcctactac aacctggccg ccgcgctgat
     2041 ccagggcgcg ggcccgaacc tcaacgccgc cacggtcgcg cgcgcggccg acgagttccc
     2101 ccccaccggg ggctggcagc agaccggcgg ccgcccggag gtggtgctgt ccgacatggg
     2161 gcccggtgac tacaccgcgg tgtcggacat caaggaggtc tactggtcgc cgaccgcccc
     2221 gtccaacatc gaccggcgcg ccggggcgta cgtgccgctg cgcggcggcc gccgctaccg
     2281 cgcgggagag ctggactcca ccttcgagat ccccgtcccg acggactagg agcggcccgt
     2341 ggg
//
LOCUS       DATMKC010000242         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_265078, whole genome shotgun sequence.
ACCESSION   DATMKC010000242 DATMKC010000000
VERSION     DATMKC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265078"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..712)
                     /locus_tag="VNU01_07040"
     CDS             complement(125..712)
                     /locus_tag="VNU01_07040"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HWH32413.1"
                     /translation="MRLPDTAHTSRPWRIHEIVPDFRLEDVWALPVSGGPDEFPALVE
                     GFAAGDPSQRLGGAARLLWALRWKLGNLFGWDDEDGGLGARVASLRERLPAGLREAAP
                     GPAFDALPFSSLYLLADEWAAEVANRTMHGVLHLGWVSDGDGGWRGQMAVLVRPNGWR
                     GSAYMAAIKPFRHWIVYPRLLRAIEREAAVRLAPE"
     gene            819..1376
                     /locus_tag="VNU01_07045"
     CDS             819..1376
                     /locus_tag="VNU01_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH32414.1"
                     /translation="MPAAPRTARDTWITEGLRTLGVGGVDAVRIEKLAQALGVTKGGF
                     YWHFQDRSALLDEMLDAWERLVVDEVIERVDADGGDPDERLRRLSKLASSSPALMGIE
                     LAVRDWARRDDGVAARLRRVDNRRMAYLRSLFGAFASDEEDAEARCLLYYSLWLGNHF
                     IAADHGGRRRGDVVKAALRLLGASA"
     gene            complement(1373..>2088)
                     /locus_tag="VNU01_07050"
     CDS             complement(1373..>2088)
                     /locus_tag="VNU01_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWH32415.1"
                     /translation="MEDVDKFFGDFRALADINLKVGRTEVVVVIGPSGSGKSTLIRCI
                     NRLEKHDRGRVVVDGVELTDDVRAIQNIRREVGMVFQQFNLFPHLTVLENVTLGPRQV
                     RRMPKRDADALAMQMLERVKIPEQAGKYPGQLSGGQQQRVAIARALAMQPKVMLFDEP
                     TSALDPEMIKEVLDAMEELAASGMTMIVVTHEMGFARAVADRVVFMAQGQIVEVGTPE
                     HFFTDPREERTKLFLSQIL"
BASE COUNT          292 a          784 c          728 g          284 t
ORIGIN      
        1 aggtcgccac ggccagcggc cgcacgatcg cctcgcgctc gcgccgccgc tcgggctccg
       61 gcacgtcgtg ccaggccacg cggcgcttct cctccagcac caacgcgcgc atccgctcct
      121 cctgtcactc cggcgcgagg cgaaccgcgg cctcgcgctc gatggcccgc agcaggcgcg
      181 ggtagacgat ccagtggcgg aacggcttga tcgccgccat gtaggccgag ccgcgccacc
      241 cgttcggccg cacgagcacc gccatctgcc cgcgccagcc gccgtcgccg tccgacaccc
      301 agcccaggtg cagcacgccg tgcatcgtgc ggttggcgac ctcggccgcc cactcgtcgg
      361 ccagcaggta cagcgagctg aacggcaggg cgtcgaacgc cgggcccggc gcggcctcgc
      421 gcagcccggc gggcagccgc tcgcgcaggg aggccacacg cgcgcccaga ccgccgtcct
      481 cgtcgtccca gccgaacagg ttgccgagct tccagcgcag cgcccacagc agccgggccg
      541 cgccgcccag ccgctgcgag gggtcgcccg cggcgaagcc ctccaccagg gccgggaact
      601 catcggggcc gccggacacc ggcagcgccc agacgtcctc caagcggaag tcggggacga
      661 tctcgtggat gcgccacggg cgcgacgtgt gcgcggtgtc gggcagccgc atcggacggc
      721 ctcctgtccc tgggggtgcc gcggcggagg atccatacgg caccgtatgc ccagacgcta
      781 ccgtatggcc agagccgctg tcgacgccgg ggaacaccat gcccgctgcc cctcgcaccg
      841 cccgtgacac ctggatcacc gaaggcctgc gcaccctggg cgtgggcggt gtcgacgccg
      901 tgcgcatcga gaagctcgcg caggcgctgg gcgtcaccaa gggcggcttc tactggcact
      961 tccaggaccg ctcggcgctg ctcgacgaga tgctggacgc ctgggagcgc ctggtcgtgg
     1021 acgaggtgat cgaacgcgtc gacgcggacg gcggggaccc ggacgagcgc ctgcggcgcc
     1081 tgtccaagct cgcctcctcc agcccggcgc tgatgggcat cgagctggcc gtgcgcgact
     1141 gggcgcgccg ggacgacggc gtcgcggcgc gcctgcgacg cgtcgacaac cggcgcatgg
     1201 cctacctgcg ctccctgttc ggcgccttcg cctccgacga ggaggacgcc gaggcgcgct
     1261 gcctgctgta ctactcgctg tggctgggca accacttcat cgccgcggac cacggcgggc
     1321 gccggcgcgg cgacgtggtc aaggcggcgc tgcggctcct gggcgcctcg gcctagagga
     1381 tctgggacag gaacagcttg gtgcgctcct cgcgcgggtc ggtgaagaag tgctcgggcg
     1441 tgccgacctc cacgatctgg ccctgcgcca tgaacaccac gcggtccgcc acggcgcgcg
     1501 cgaagcccat ctcgtgggtg accacgatca tcgtcatgcc ggaggcggcc agctcctcca
     1561 tcgcgtccag gacctccttg atcatctccg ggtccagcgc ggaggtgggc tcgtcgaaca
     1621 gcatcacctt gggctgcatc gccagcgcgc gcgcgatggc cacgcgctgc tgctggccgc
     1681 cggagagctg accgggatac ttgccggcct gctcggggat cttcacgcgc tccagcatct
     1741 gcatcgcgag cgcgtcggcg tcgcgcttgg gcatgcggcg cacctgccgg ggccccagcg
     1801 tcacgttctc cagcaccgtc aggtggggga acaggttgaa ctgctggaac accatgccga
     1861 cctcgcggcg gatgttctgg atggcgcgca cgtcgtcggt gagctccacg ccgtcgacca
     1921 ccacgcggcc gcggtcgtgc ttctccagcc ggttgatgca gcggatcagc gtcgacttgc
     1981 ccgagcccga cgggccgatc accacgacca cctcggtgcg cccgaccttg aggttgatgt
     2041 cggccagcgc gcggaagtcg ccgaagaact tgtcgacgtc ctccatga
//
LOCUS       DATMKC010000243         4159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_7503, whole genome shotgun sequence.
ACCESSION   DATMKC010000243 DATMKC010000000
VERSION     DATMKC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7503"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VNU01_07055"
     CDS             complement(<1..1753)
                     /locus_tag="VNU01_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32416.1"
                     /translation="MGTADTVVDLAELAVSGLPVGGVSVGEVRSFASTDTDPTYNPVG
                     KGKPFAIAKVSLPDGSGQSVRSDGDTGAEGKTVPISGVGSLTVGDLTATAGQDAARSV
                     VNAVEGQLDAVVAGLKARAAGVSAKVGADGSSTTNGAVIEHFSVGLTDVLPADLLAQL
                     PLDKLIALADGLGIKVDLGPLNDAVDQVRELAAAVDEVVGTEKAIEDLETQIDVLRAT
                     MGSGTAAAQSAVDQAQAGVDAAQGELDSLNSEHASLVSQYASAGCTTLPALPGCSDIA
                     AQIAAVEQEIAAQEGVVAAKQQVLSEAQAALTVAREGAQTVQAEIDALQAEVDALVAS
                     IDDLLDTIVDLAKKVVKLDLEGILEALLAGLDGVELIGVDRMSFGVSTMATATSSKAT
                     TLCDVAGVRILGRARQVADCDDVQAAVGEVEATLKGVLAALPIQGVVPANAVNVEGPR
                     MSTSPEDYAAEGYRVASAKVSGLDLTVEPIKLTQVVDSLVVQARALVSGTLDKIKEVT
                     GVEVPVDVNAALDDLLAQLNGLPIGDTLDGVTTPRVAISAIDLGSRSSFRAAGTGIDG
                     HPVDGSPVTPAGDLPHTG"
     gene            complement(3057..3341)
                     /locus_tag="VNU01_07060"
     CDS             complement(3057..3341)
                     /locus_tag="VNU01_07060"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HWH32417.1"
                     /translation="MVQELSADQFQGPPEYYSDSVRIGSNPYGIVFEFGLTTNSPGDA
                     RAQAVVRMSPQHALVFYQLIRKHLRLYEANVGKIALPEALYAELDIDGEI"
     gene            complement(3502..3966)
                     /locus_tag="VNU01_07065"
     CDS             complement(3502..3966)
                     /locus_tag="VNU01_07065"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HWH32418.1"
                     /translation="MSGARARQAVADWFAGQPELSAERVGDGWLTVLAGERKRTLPVL
                     VEVGERTLSLQAFFMTAPEEAQADLYAFFLRRHLRSYVVRFALDADGDVLLVGVLPVE
                     AVTVEELDRVLGQLLQLADDTYLEAVRMGFASYIEREQAWREKAGLGRNPIS"
     gene            complement(3963..>4159)
                     /locus_tag="VNU01_07070"
     CDS             complement(3963..>4159)
                     /locus_tag="VNU01_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32419.1"
                     /translation="PEAWARATLPYLLDPARRAAAGRAGRLHAEGHGWARTAESTLEV
                     YRAVVEEEAPAQVLSAPCGA"
BASE COUNT          625 a         1465 c         1414 g          655 t
ORIGIN      
        1 cgcccgtgtg gggcaggtcc ccggccggcg tgaccgggct gccgtcgacc gggtggccgt
       61 cgatacccgt cccagcggcg cggaagcttg agcgcgagcc caggtcgatc gcgctgatcg
      121 cgacgcgggg cgtcgtgacg ccgtccaggg tgtcaccgat cggcaggccg ttgagctggg
      181 ccagcaggtc gtccagggcg gcgttgacgt ccaccggcac ctccacgccg gtgacctcct
      241 tgatcttgtc cagcgtgccg ctcaccagcg cgcgagcctg caccaccagg ctgtcgacca
      301 cctgggtcag cttgatcggc tcgaccgtga ggtccaggcc gctgaccttg gccgaggcga
      361 cccggtagcc ctccgccgcg tagtcctcgg gcgaggtgga catccgcggg ccctcgacgt
      421 tgaccgcgtt ggccggcacg acgccctgga tgggcagggc cgcgagcacg cctttcagcg
      481 tggcctcgac ctcacccacg gccgcttgca cgtcgtcgca gtcggcgacc tggcgcgccc
      541 ggcccaggat ccggacgccg gcgacgtcgc acagggtggt ggccttgctg gaggtggccg
      601 tggccatcgt gctgacgccg aaggacatgc ggtccacgcc gatcagctcc acgccgtcca
      661 ggccggccag cagcgcctcg aggatgccct ccaggtccag cttcacgacc ttcttggcga
      721 ggtccacgat ggtgtcgagc aggtcgtcga tcgaggcgac gagcgcgtcg acctccgcct
      781 gcagcgcgtc gatctcggcc tgcaccgtct gcgcgccttc ccgggccacg gtcagggcgg
      841 cctgcgcctc ggacagcacc tgctgcttgg cggcgacgac gccctcctgg gcggcgatct
      901 cctgctccac cgccgcgatc tgcgccgcga tgtcactgca gccgggcagc gccgggagcg
      961 tcgtgcagcc cgcgctggcg tactgggaca ccagcgaggc gtgctcgctg ttcaggctgt
     1021 cgagctcgcc ctgggcggca tcgacgcccg cctgcgcctg gtccaccgcg gactgggcgg
     1081 ccgcggtgcc gctgcccatc gtggcgcgca gcacgtcgat ctgggtctcg aggtcctcga
     1141 tggccttctc cgtgccgacg acctcgtcga ccgccgcggc cagctcgcgc acctggtcga
     1201 ccgcgtcgtt gagcgggccg aggtccacct tgatgcccag gccgtcggcc agggcgatca
     1261 gcttgtccag cgggagctgg gccagcagat ccgcgggcag cacgtcggtg agccccacgg
     1321 agaagtgctc gatgacggcg ccgttggtgg tggaggagcc gtcggcgccg accttggcgc
     1381 tcacaccggc ggcacgggcc ttcaggcccg ccaccaccgc gtcgagctgc ccttcgacgg
     1441 cgttgacgac cgaccgcgcg gcgtcctggc cggcggtcgc ggtgaggtca ccgacggtga
     1501 gcgagccgac gccgctgatc ggcacggtct tgccctctgc accggtgtcc ccgtcgctgc
     1561 gcacggactg gccggagccg tccggcagcg aaaccttggc gatggcgaag ggcttgccct
     1621 tgccaacggg gttgtacgtg gggtcggtgt cggtcgacgc gaacgagcgg acctccccga
     1681 ccgagacgcc tcccacgggc aggccgctga cggccagctc ggcgaggtcg acgacggtgt
     1741 cggcggttcc catggggacc acgacggtgg acgcgttggc gctggtcccg gcaagtgccg
     1801 aaagcgccac agccgcaaca gcggcaatgt accggcgatt catgctgcac cccttgcgcg
     1861 tcatgggagc tccccgtgga gctccttgtg ggggcctcgc acctggacct gtcgagcagg
     1921 ccgtgcctca gatgttcggc tccctacagg cttcggtggc aggacgaagc cgcgacatga
     1981 cgcgcaggga tggttttggc atggctgaca gggactagcc cgccgggcaa atcatggcaa
     2041 ctagagcgat gtgtcacctg acaagcctgg gtccgcgtcg gcttagggcg ttggccgact
     2101 ggcttcgagg ccagcgaccg cgcggagtgc gtccagccgg gcgtctaccg cgcggcgtgg
     2161 atctttggat cattcctcgt agagcgcgtc cggtgctagc tacgcccgta ggtccgtgtc
     2221 ggcgacaagc gcgagccttc cctagacggt tcacgatggg accgcagtca gcgcggaggc
     2281 gggtaggacg ctatggagtt tcatcgtccc tcccctgcac tgtcggcggg cacagcgcct
     2341 tgagccctgt tagccccggc ccaggaacgg attcctgccg ccctcacacc gctcgcgcac
     2401 caaggtcagc cagccgtcgc tgtctagcgt gcagtcgacc aactcccaga actctctagg
     2461 gcggagcgcg cactgccgag ccatcaggcc cgctaggaaa gagtcgatct ctcggacgcc
     2521 gtggcttacg cgcgtcacga gggtggttac gccgtccacg tccatcctga gcatgacgtg
     2581 gtggctgtcc tcccgcgcca tgcccttagc gcagagcgcg ttgattaccg ttgctgcccc
     2641 ccgaaccggc acgagacctc taggcgccca aagaggtcaa gcgagcaagc agtgccatga
     2701 ggtccggact caagtccgat tggcgcgcga gggtgtcgcg aaactcaatc aaagcttcgt
     2761 agaagtcctg agtcgcttca gcgatggtgt cgccgacgcc gtgaatggcg gagatgggtt
     2821 caacgattgc ggtatacccc cccgtccggc gtacttggac acggaggggc aacggcaagg
     2881 gccggccccg cggaccctct gtcaacaccg tttcgtacat gaatgcctca gccgtgcccg
     2941 aagtggcacc aaaccacgca tcacgtggcc cggtcagtga cgagtccaat gccctttcat
     3001 cgctctcgac cacgaggcgg cgcgcggaag tcgactgctg tgtcaaagtc accgctctag
     3061 atctccccgt cgatgtccag ctcggcgtac agggcttcag gcagggcaat cttcccaacg
     3121 ttagcttcgt acagccgtag atgcttacgg atcaactggt agaagacgag cgcatgttgc
     3181 gggctcatgc gaaccaccgc ttgagcgcgg gcgtccccag gtgagttggt cgtcagcccg
     3241 aattcgaaca cgatcccgta ggggttgcta ccgatgcgca cagagtcgct gtagtactcg
     3301 ggcggtccct ggaactggtc agccgacaac tcctgcacca ctgtccccct agcgtcgctg
     3361 tgagggtcgc ataggacatg tcggcgggga ggcctgccca ctgaacccct tgcggcggaa
     3421 gcctaaggct ttccggcctc caggcaaagg ccccttgggt ttgtcaggtg gcacatcgcc
     3481 caaataggca tatctcgccc gtcaggagat ggggttgcgg cccaggccgg ccttctcccg
     3541 ccacgcctgc tcgcgctcga tgtaggaggc gaagcccatg cgcaccgcct ccaggtaggt
     3601 gtcgtcggcg agctggagca actggccgag gacgcggtcc aactcctcca ccgtgaccgc
     3661 ctccacgggc agcacgccca ccagcagcac gtcgccgtcg gcgtccaggg cgaagcgcac
     3721 gacgtagctg cgcaggtgcc ggcgcaggaa gaaggcgtac aggtcggcct gggcctcctc
     3781 cggcgccgtc atgaagaacg cctgcaggct cagcgtgcgt tcgcccacct ccaccagcac
     3841 cggcagcgtc cgcttgcgct caccggccag gacggtcagc cagccgtcgc cgacacgctc
     3901 cgcggacagc tccggctggc ccgcgaacca gtcggccacc gcctggcggg cgcgcgcgcc
     3961 gctcatgcgc cgcagggcgc cgacagcacc tgcgcgggcg cctcctcctc cacgacggcg
     4021 cggtacacct ccagcgtgga ctcggcggtg cgcgcccacc cgtgtccttc ggcgtgcagg
     4081 cggccggccc ggccggcggc ggcacggcgt gccgggtcca gcaggtacgg caacgtggca
     4141 cgcgcccagg cctccgggt
//
LOCUS       DATMKC010000244         4495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_194894, whole genome shotgun sequence.
ACCESSION   DATMKC010000244 DATMKC010000000
VERSION     DATMKC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194894"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VNU01_07075"
     CDS             <1..723
                     /locus_tag="VNU01_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWH32420.1"
                     /translation="VVDRRGEAHEVRLTAAVYDGSRWRHLEHRFTYRKLDLARGDTGA
                     AVRDLQQRLGAQGFWAGAPDGVYGTLTHQAVMALEKAHGLPRDGVYDARARQILEAGA
                     ARPRGRTTAGRWLEVDLERQLVLAVLDGHVQWAFNASTGHGRWYEFNGRTYRATTTTG
                     QRRVVREIDGMREAERGLLWRPKYFDDSRGIAVHGSTSVPAYPASSGCVRLSYPAMDF
                     IWSAGLAPVGTGVWVYPENHYG"
     gene            complement(720..1154)
                     /locus_tag="VNU01_07080"
     CDS             complement(720..1154)
                     /locus_tag="VNU01_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HWH32421.1"
                     /translation="MTAAGKGTPASGRGAQGGDGLVAKVDAVRDARERLERDVDLMTA
                     ELKATVGVTMEKSIWKAVTIGAGVLAGALVRKLLNAAWRGATHADPPTDPYQPAGWGE
                     AVTWAAASGIGIGVARLVAARGAAAGWHKAMGAPPPGVSAPV"
     gene            complement(1151..1627)
                     /locus_tag="VNU01_07085"
     CDS             complement(1151..1627)
                     /locus_tag="VNU01_07085"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWH32422.1"
                     /translation="MNTAVKSLTNRTNTAPAGAPPLGAALRRRFGPPPTVGAGAAAKA
                     VAEDASRLVRAELDLAKAELSLGAKEKGLGIGLGVAACTFGFLALQALLVTAGLALAL
                     VLPAWAAAGVVAAFLLLLTAALGLVAMSHLRRPVGLDTTKETIEEDVEWAKRRLRR"
     gene            1989..2933
                     /locus_tag="VNU01_07090"
     CDS             1989..2933
                     /locus_tag="VNU01_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH32423.1"
                     /translation="MTATVAQMVEEMPLHDHVQLYLREMARTALLTAEEEVDLAKRYE
                     AGLEAERLLAERRKMTPTRRRQLHQVDRDGKRAKERLVQANLRLVVSVAKRYQGQGLP
                     LLDLIQEGNLGLLRAVEKFDYRRGYKFSTYATWWIRQAVGRGVADKGRTIRLPVHMME
                     RVRRALSMQRDLAEALGREPSIEELAEELGEEIAIVEELLTYARTPTSLETPVGEDGD
                     AELGDFIEDRNAEDPLEMAAKGLARQELLDVVAGLPERERIILELRFGLLDGEARTLD
                     DVGRYFGLTRERIRQLEARALSKLRHPSRGRALSDTAA"
     gene            complement(3015..4163)
                     /locus_tag="VNU01_07095"
     CDS             complement(3015..4163)
                     /locus_tag="VNU01_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32424.1"
                     /translation="MTIRVVLADGQGIEFDPTPGRVMLARSDHTFAELADAIDTAFGR
                     WDPSPSHEFEVEGRLVASDPTAVPVLMPDAAPEDSDARALEDVGLRPGSRFTYLFDPS
                     QRWVHRCRVEQSGLDPFTLIEEEPDVPVPVFGWGDVPDQYGRIDEDDEPYLPDDALAE
                     VLEEALSEDDDLEVVLDLDEAGRDDDDEDEDDDDDAVRWQAPEPSSWDVVAQALEGLK
                     VPRPDEELREAVDRLHLHSDDEDWPWGVLWAAGGLEDDELPSDDEELWLELASGVVVP
                     REALPLEPEVEEAWAALEPADWAGAVIGLARGGAGQDASPGALLALIGDCAEVEASDL
                     DEDEEAALAAGFETVVALWRALGAVDDKGRLTALGHWGLPRALERAWT"
     gene            4287..>4495
                     /locus_tag="VNU01_07100"
     CDS             4287..>4495
                     /locus_tag="VNU01_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32425.1"
                     /translation="MPVASSVPLRAAAVVLLSGVGNDAGSPADARRVALAAARDGAWA
                     LPQEWLGVGETEVQAAARVAPGATRA"
BASE COUNT          598 a         1720 c         1596 g          581 t
ORIGIN      
        1 gtcgtagacc gccgcggtga ggcgcacgag gtgcgcctca ccgcggcggt ctacgacggc
       61 tcgcgctggc gccacctgga gcaccggttc acctaccgca agctggatct ggcgcgcggc
      121 gacaccggcg cggcggtgcg cgacctgcag cagcggctgg gcgcgcaggg cttctgggcc
      181 ggcgcgccgg acggcgtcta cggcacgctg acgcaccagg cggtcatggc gctggagaag
      241 gcgcacggcc tgccgcgcga cggggtctac gacgcgcgcg cccgccagat cctggaggcg
      301 ggggccgcca ggccgcgcgg gcgcaccacg gccggccgct ggctggaggt cgacctggag
      361 cgccagctcg tcctggcggt gctggacggg cacgtgcagt gggcgttcaa cgcctccacc
      421 ggccacggcc gctggtacga gttcaacggc cggacgtacc gggcgaccac cacgacgggc
      481 cagcggcgcg tcgtgcgcga gatcgacggc atgcgcgagg cggagcgcgg cctgctgtgg
      541 cgccccaagt acttcgacga ctcccggggc atcgcggtcc acggctcgac cagcgtgccg
      601 gcctacccgg cgtcatccgg ctgcgtccgc ctgagctacc cggcgatgga cttcatctgg
      661 tcggcgggcc tggccccggt cggcaccggc gtctgggtgt atcccgagaa ccactacggc
      721 tagaccggag ccgacacgcc ggggggcggg gcgcccatcg ccttgtgcca cccggccgcc
      781 gcgccgcgcg cggccaccag cctggccacg ccgatgccga tgccgctggc ggccgcccac
      841 gtgaccgcct cgccccagcc ggcgggctgg taggggtccg tgggcgggtc ggcgtgcgtg
      901 gcgccgcgcc acgccgcgtt cagcagcttg cgcacgagcg cgccggccag cacgccggcg
      961 ccgatggtca cggccttcca gatggacttc tccatggtca ctcccacggt cgccttcagc
     1021 tcggcggtca tcaggtcgac gtcccgctcc agccgctcgc gggcgtcccg gacggcgtcg
     1081 accttggcga ccaggccgtc accgccctgc gcgccgcggc cggacgcggg cgtgcccttg
     1141 ccggcggcgg tcatcggcgc agacgtcgtt tggcccactc gacgtcctcc tcgatggtct
     1201 ccttggtggt gtccaggccc acgggccggc gcaggtggct catggccacc aaccccagcg
     1261 ccgcggtgag cagcagcagg aacgccgcca cgacgcccgc ggccgcccag gccggcagca
     1321 ccagcgccag ggcgagtccg gcggtcacca gcagcgcctg cagggccagg aacccgaagg
     1381 tgcacgccgc cacgcccaag ccgatcccga ggcccttctc cttggcgccc agggacagct
     1441 cggccttggc caggtcgagc tccgcccgca ccagccggct cgcgtcctcg gcgacggcct
     1501 tggcggccgc cccggcgccc accgtgggcg gcggcccgaa ccggcgccgc agcgccgcgc
     1561 ccagcggcgg cgcccccgcc ggtgcggtat ttgtccgatt cgttagggat ttgacagcgg
     1621 tgttcatgtc gggcgtctac ccggaggcag gaatggggac acccttccac gaagcttcac
     1681 cgcgacggcg ccgtgccgtc atgggacatg gacgctgcga attcgcaccg gcagcgtgaa
     1741 aggctcacgt taggatgggc tgtccaggaa caaaacactt gccagacaca ggggtcctcg
     1801 tcgtacgctt gcccgtggag cggccccttc ccccggggag cgctgtcgac ttgtgcgcac
     1861 accacgtgtc gcggccggtc aatggctttc ccgcgcttct cacctggcac cggctcgtta
     1921 gaaccggcgc ctgacccccg taccagcgtc aagagcgctc ccgcgccgcg cgagacaacg
     1981 taaggaaggt gacggccacc gtggcccaga tggtcgagga gatgccgctg cacgaccacg
     2041 tgcagctgta tctgcgagag atggcccgaa ccgcgctcct caccgcagag gaggaggtcg
     2101 atctcgcgaa gcgctacgag gccggtctgg aagcggagcg actgctcgcc gagcgcagga
     2161 agatgacgcc cacgcggcgc cgccagctgc accaggtgga ccgcgatggc aagcgcgcca
     2221 aggagcgcct ggtccaggcc aacctccgcc tggtggtctc cgtcgccaag cgttaccagg
     2281 gccagggcct gcccctgctg gacctgatcc aggagggcaa cctgggcctg ctgcgcgcgg
     2341 tcgagaagtt cgactaccgc cgcggctaca agttctcgac ctacgccacc tggtggatcc
     2401 gccaggccgt gggccgcggt gtggccgaca agggccgcac catccgcctg cccgtccaca
     2461 tgatggagcg cgtgcgccgc gccctgtcga tgcagcgcga cctcgccgag gcactcggcc
     2521 gcgagccctc catcgaggag ctcgccgagg agctgggcga ggagatcgcc atcgtcgagg
     2581 agctgctcac ctacgcgcgc acgcccacgt ccctggagac cccggtgggt gaggacggcg
     2641 acgcggagct gggcgacttc atcgaggacc gcaacgccga ggatccgctg gagatggccg
     2701 ccaaggggtt ggcccgccag gagctgctcg atgtcgtggc cggcctgccg gagcgcgagc
     2761 gcatcatcct ggagctgcgc ttcggcctgc tcgacggcga ggcgcgcacc ctggacgacg
     2821 tcggccgcta cttcgggctg acgcgcgagc gcatccgcca gctggaggcg cgggcgctgt
     2881 ccaagctgcg ccaccccagc cgcggccggg ctctctccga caccgccgcc tagccacagc
     2941 ttcacccccg aggcgggtgc attccgacgc agataggcgt cgaggtgcac ccgcttcggc
     3001 gttctggctg tggatcaggt ccaggcgcgc tccagcgccc gaggcagccc ccagtggccc
     3061 agggcggtga gccggccctt gtcgtccacg gcgcccagcg cccgccacag cgccaccacg
     3121 gtctcgaagc cggccgccag cgccgcctcc tcgtcctcgt ccaggtcgct cgcctcgacc
     3181 tcggcgcagt ccccgatgag cgcgagcaga gcccccgggc tggcgtcctg cccggcgccg
     3241 ccgcgcgcca ggccgatcac ggccccggcc cagtcggcgg gctccagcgc cgcccacgcc
     3301 tcctcgacct cgggctccag gggcagggcc tcgcgcggca ccacgacgcc ggacgcgagc
     3361 tccagccaca gctcctcgtc gtccgatggc agctcgtcgt cctccaggcc gcccgccgcc
     3421 cacagcacgc cccagggcca gtcctcgtcg tcggagtgca ggtgcagccg gtccacggcc
     3481 tcgcgcagct cctcgtccgg ccgcggcacc ttcagcccct ccagcgcctg cgcgacgacg
     3541 tcccaggagg acggctcggg cgcctgccag cgcacggcgt cgtcgtcgtc gtcctcgtcc
     3601 tcgtcgtcgt cgtcccgccc ggcctcgtcg aggtcgagga ccacctccag gtcgtcgtcc
     3661 tcggaaagcg cctcctccag cacctccgcg agggcgtcgt cgggcaggta cggctcgtcg
     3721 tcctcgtcga tgcggccgta ctggtccggg acgtcgcccc agccgaacac cggcaccggc
     3781 acgtccggct cctcctcgat gagggtgaac gggtccagac cggactgctc cacgcggcag
     3841 cggtgcaccc agcgctggct ggggtcgaac aggtaggtga agcgggaccc gggccgcagg
     3901 cccacgtcct ccagcgcgcg cgcgtcgctg tcctcggggg ccgcgtcggg catgagcacc
     3961 gggacggcgg tggggtcgct ggcgaccagc cggccctcga cctcgaactc gtgcgacggg
     4021 ctggggtccc agcgcccgaa cgccgtgtcg atcgcgtcgg ccagctcggc gaacgtgtgg
     4081 tcggagcgcg cgagcatcac ccgccccggc gtgggatcga actcgatccc ctggccgtcg
     4141 gcgagcacca cacggatcgt catccactcc gccatgcctg ctccctcgtt gccggaccgc
     4201 gtcacgctag cgcctggagc gccacgaggg gacacggttt ttccccgcgt gtggcggaag
     4261 aagcccccgg cccgcacaat cccggcatgc ccgtggcctc ttccgtcccc ctgcgcgccg
     4321 ccgccgtggt gctgctgagc ggtgtcggca acgacgccgg gagccccgcc gacgcgcgac
     4381 gggtggcgct ggcggccgcg cgcgacggag cctgggcgct gccgcaggag tggctgggcg
     4441 tcggggagac ggaggtgcag gccgcggcgc gggtggcgcc gggcgccacc cgcgc
//
LOCUS       DATMKC010000245         3216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_13537, whole genome shotgun sequence.
ACCESSION   DATMKC010000245 DATMKC010000000
VERSION     DATMKC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13537"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VNU01_07105"
     CDS             <1..228
                     /locus_tag="VNU01_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32426.1"
                     /translation="APAASRYEALARDLPRLAAEWANGNGSWAAERPLVAAAPPQPAE
                     HVPGQLAIPVGEAESGDDAVVVSIELARRSP"
     gene            300..620
                     /locus_tag="VNU01_07110"
     CDS             300..620
                     /locus_tag="VNU01_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32427.1"
                     /translation="MSLPRELVKAVRRLDEAQLRRLAILVRGLLETSDGPRIEVGDLP
                     GMPHVTYRQEHVRCGRDCAACPHGPYWYAYWKEGGRSRSQYIGRDLPADVRRLVEQRE
                     RARA"
     gene            672..866
                     /locus_tag="VNU01_07115"
     CDS             672..866
                     /locus_tag="VNU01_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32428.1"
                     /translation="MTIRASAREAHVGAEGAWRRTVDRRLSHLEVDVATLKNDVAWIR
                     RLGGVMLTLQVATIAAVLRA"
     gene            876..1064
                     /locus_tag="VNU01_07120"
     CDS             876..1064
                     /locus_tag="VNU01_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32429.1"
                     /translation="MCNDVEVKGTDMDQDWRDWRRGVDERLGDLTTRVAVLTRDLRWI
                     GGLILVVLMAQAGADLNR"
     gene            1157..2509
                     /locus_tag="VNU01_07125"
     CDS             1157..2509
                     /locus_tag="VNU01_07125"
                     /inference="COORDINATES: protein motif:HMM:NF007088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWH32430.1"
                     /translation="MADLSRLHAIVKAYDIRGVVPDDLDEDVAERLGRAAAIELQGEA
                     VVVARDMRPSGEALAGAFMRGVRAEGVDTVDIGLASTDLLYYASGRLGLPGAMWTASH
                     NPARYNGLKLCRAGAEPVSLDTGLSAIRDRAVAGQFAPAGQEGRHERRELLGEYAEHV
                     LGFVDAGAMRPLKVAVDAGNGMGGLVVPAVFEGLPLEVVPLYFELDGTFPNHPANPIE
                     PENLVDLQRAVITHGCDIGLAFDGDADRMFCVDEEGRPVSPSLVTAIIAERLLRKNPG
                     ETVLYNLICSKVVPETVEAAGGHAVRTRVGHSFIKAVMRDTGAVFAGEHSGHYYFRDN
                     FRADSGLIAAVVLLEALGEHGGPLSALVKPYDRYAASGELNTEVADQAGAMDRVAEAY
                     VGRGEVDWADGLTVNGDGWWFNLRPSNTEPLLRLNVEADGEQAMAQVRDEVLDIVRQS
                     "
     gene            2575..2772
                     /locus_tag="VNU01_07130"
     CDS             2575..2772
                     /locus_tag="VNU01_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWH32431.1"
                     /translation="MAVDAGLVELLVCPDCHGAVEYKDRRGLIICTECNLHYPVRDGI
                     PVMLVEEATRPGRRASRDGGS"
     gene            2769..>3216
                     /locus_tag="VNU01_07135"
     CDS             2769..>3216
                     /locus_tag="VNU01_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32432.1"
                     /translation="MSRTDLRVDLDRHWEFGGVDPADALGDLEVSPGQWAAAGELRVP
                     ALDLGGVDHVVLTGMGGSGISSDIVAELGAARLPVPLIVHKGYGLPAYVGERSLLVAV
                     SCSGDTEETRLAAETALDRGARLFAVARGGRLSELCHERGLPWVRIA"
BASE COUNT          426 a         1148 c         1213 g          429 t
ORIGIN      
        1 gcgccggcgg ccagccgcta cgaggcgctg gcgcgcgacc tgccgcgcct ggccgccgaa
       61 tgggccaacg gcaacggctc ctgggccgcc gagcggccgc tggtggcggc ggccccgccg
      121 cagccggccg agcacgtgcc gggccagctc gccatcccgg tcggcgaggc ggagtccggg
      181 gacgacgccg tggtcgtgtc catcgagctc gcccgccgct ctccctgacc aatgcgtgcc
      241 ggcatggcgc gtcaaggctt gacgttgacc actgtgactt cttatgtgcg gacatccgga
      301 tgagcctgcc ccgtgagctg gtcaaggcgg tgcgccgcct ggacgaggcg cagctgcggc
      361 gcctggccat cctggtccgt ggcctgctgg agacctccga cggaccgcgc atcgaggtcg
      421 gcgacctgcc cgggatgccc cacgtcacct accggcagga gcacgtgcgc tgcggcaggg
      481 actgcgcggc gtgcccccac ggcccctact ggtacgcgta ctggaaggag ggcggtcgca
      541 gccgctccca gtacatcggg cgggacctgc ccgccgacgt gcggcgcctg gtcgagcagc
      601 gcgagcgcgc ccgggcgtag gcctgtggac agcgggcgac gcgttcgccg ggcctggggt
      661 agcgtcgccg catgacgatc cgggcatcgg cacgggaggc gcacgtgggc gcggaaggcg
      721 cgtggaggcg gacggtcgac cgtcggttgt cccacctcga ggtggacgtg gcgaccctga
      781 agaacgacgt cgcctggatc cgccgcctgg gcggcgtgat gctgacgctc caggtcgcca
      841 ccatcgcggc cgtcctccga gcatagggat ccccgatgtg caatgacgtc gaggtgaagg
      901 gcacggacat ggatcaggac tggcgggact ggcgacgcgg cgtcgacgag cggcttggcg
      961 acctcacgac gcgagtcgcg gtgctcacgc gcgacctccg ctggatcggc gggctcatcc
     1021 tcgtggtcct gatggcgcag gcgggcgcgg acctgaaccg ctgacagcgt gtgcgcggga
     1081 ccgtgagggg aatggtgcct cgcgccggag cctctaggat tccggcgccg tcccccctgc
     1141 gaggagaagc gcacccgtgg ccgacctgtc ccgcctgcac gccatcgtca aggcctacga
     1201 catccgcggc gtcgtgcccg acgacctcga cgaggatgtc gccgaacgcc tgggccgcgc
     1261 ggcggcgatc gagctgcagg gggaggcggt cgtggtggcc cgcgacatgc gccccagcgg
     1321 cgaggcgctc gccggggcgt tcatgcgggg ggtccgcgcc gagggcgtgg acaccgtgga
     1381 catcggcctg gcctccaccg acctgctcta ctacgcctcc ggccggctgg gactgcccgg
     1441 ggccatgtgg accgccagcc acaacccggc ccgctacaac ggcctgaagc tgtgccgggc
     1501 gggcgccgag cccgtgtcgc tggacaccgg cctgtcggcg atccgcgacc gcgccgtggc
     1561 gggccagttc gcgcccgccg gccaggaggg ccgccacgag cgccgcgagc tgctcggcga
     1621 gtatgccgag cacgtgctcg gcttcgtgga cgccggcgcc atgcggccgc tgaaggtcgc
     1681 ggtcgacgcg ggcaacggca tgggcggcct ggtggtcccc gccgtgttcg aggggctgcc
     1741 gctggaggtc gtgccgctgt acttcgagct ggacggcacc ttcccgaacc atcccgcgaa
     1801 ccccatcgag ccggagaacc tcgtcgacct ccagcgcgcc gtcatcaccc acggctgcga
     1861 catcggcctg gccttcgacg gcgacgccga ccgcatgttc tgcgtggacg aggaaggccg
     1921 cccggtgtcg ccgtccctgg tgacggcgat catcgccgag cgcctgctgc gcaagaaccc
     1981 gggcgagacc gtgctctaca acctgatctg ctccaaggtg gtgccggaga cggtggaggc
     2041 cgcgggcggg cacgccgtgc gcacgcgcgt gggccacagc ttcatcaagg cggtcatgcg
     2101 cgacaccggc gccgtgttcg ccggggagca ctccggccac tactacttcc gcgacaactt
     2161 ccgcgccgac tccgggctca tcgccgcggt ggtcctgctg gaggcgctcg gcgagcacgg
     2221 cgggccgctg tcggcgctgg tgaagcccta cgaccgctac gccgcctcgg gggagctcaa
     2281 caccgaggtc gccgaccagg ccggggcgat ggaccgtgtc gccgaggcct acgtcggccg
     2341 cggcgaggtg gactgggccg acggactgac ggtcaacggc gacggatggt ggttcaatct
     2401 gcgtccgtcg aacaccgagc cgctgctgcg cctgaacgtg gaagccgacg gcgagcaggc
     2461 gatggcgcag gtgcgcgacg aggtgctcga catcgtgcgc cagtcctgac gcggtcctga
     2521 cccggagcgg ccgcacgccg caccggtccg gcagacgacg acaaggagtg agacgtggcg
     2581 gttgacgcgg gtctggtgga actgctcgtg tgcccggact gccacggggc ggtcgagtac
     2641 aaggaccgcc gcgggctgat catctgcacc gagtgcaacc tgcactaccc ggtgcgcgac
     2701 ggcatcccgg tgatgctggt cgaggaggcc acgcgccccg ggcggcgcgc ctcgcgcgac
     2761 ggcggctcgt gagccggacg gacctgcgcg tggacctgga ccgccactgg gagttcggcg
     2821 gcgtcgaccc cgccgacgcg ctgggcgacc tggaggtctc acccggccag tgggccgccg
     2881 ccggcgagtt gcgcgtgccg gccctggacc tcggcggcgt cgaccacgtc gtgctgaccg
     2941 gcatgggcgg ctccggcatc tccagcgaca tcgtcgccga gctgggcgcc gcccggctgc
     3001 ccgtgccgct gatcgtgcac aagggctacg ggctgcccgc ctacgtcggc gagcgcagcc
     3061 tgctggtcgc cgtgtcctgc tccggcgaca ccgaggagac ccggctggcc gcggagaccg
     3121 cgctcgaccg cggcgcccgc ctgttcgcgg tggcgcgtgg cggccgcctg tccgagctgt
     3181 gccacgagcg cggcctgccg tgggtccgca tcgcca
//
LOCUS       DATMKC010000246         8826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_300738, whole genome shotgun sequence.
ACCESSION   DATMKC010000246 DATMKC010000000
VERSION     DATMKC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300738"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VNU01_07140"
     CDS             <1..1306
                     /locus_tag="VNU01_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32433.1"
                     /translation="LERFRQSIDVGALRDGVTTPVPDVRGYSVRLFASRQELGEGMGT
                     SFPQYRGRWQPYAVWVPAGYEAGTPNPLLLSLHSLSVHHNQYRGGTSPSASYKTYYEQ
                     LEAGLGAIVVTPLGRGPDGWYEDEGLVDTLEVWADVQRRFSVDRERTHVGGYSMGGYG
                     TYRLATLMPDAFASAVSIVGPPANGIWAYPLPPTGGEANPDNTRPQLENTRHVPFWIT
                     QGVLDELVPVAGVVHQARRFEELGHEFRLAVHPAEDHLSFVFKDEWSREVAWLAAHPR
                     RVTSPHCVTLKVRPASLATGGRPEILAHVAALTAEVGARVDGAYWVGDVAVAAGADVT
                     GVVDLSSGAVTRRQAGVSPVATAGVDGPSPHTLTGQDKVWAEGPPAQTLTGSLSNVTA
                     LTVDLDRAGLAGVPQVDVTTDTPVTLTFTSGGVPVGQVSLAP"
     gene            complement(1313..1900)
                     /gene="yihA"
                     /locus_tag="VNU01_07145"
     CDS             complement(1313..1900)
                     /gene="yihA"
                     /locus_tag="VNU01_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWH32434.1"
                     /translation="MASALKLEFVMSAPRLADLPPTAAEVALAGRSNVGKSSLLNALA
                     SARKLATVSSKPGSTKLLNFFVTPQGTSLVDLPGYGYASTASARMREAWLRRTERYLL
                     QREGLRLVLLLVDGEIGPTSLDQEVIAWLRDHGVPFQIVATKHDKVKASKRDRRKRDL
                     PAACGVAPSEVIWVSAERNVGIDRLRELVRETLSA"
     gene            complement(1932..2014)
                     /locus_tag="VNU01_07150"
     tRNA            complement(1932..2014)
                     /locus_tag="VNU01_07150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1979..1981),aa:Leu,seq:tag)
     gene            complement(2059..2298)
                     /locus_tag="VNU01_07155"
     CDS             complement(2059..2298)
                     /locus_tag="VNU01_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32435.1"
                     /translation="MLIATEHTRAPRAPELHTGPRDTSTPRGRSLVAVALVLLALAAI
                     VLLAQSGASGATLFTAVLVNAAVAPLAAARRLPTA"
     gene            complement(2369..3106)
                     /locus_tag="VNU01_07160"
     CDS             complement(2369..3106)
                     /locus_tag="VNU01_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWH32436.1"
                     /translation="MCGRFVSITEPDGLVRFFTVDERKADELPPRYNVAPSQPVYAVA
                     SHAAERLLVGFQWGLVPHWASDPKIGNKLINARAESVAEKPAFRDAFKRKRCLIPADG
                     FYEWKRLGDKRKVPYFIHHADGLPLAFAGLWSAWRERDLPEDAPDDAGWLRTCTIVTT
                     DAGERIRELHTRMPVILPEPLWDAWLDADLRDPAGLERILAAATDEPLVWHPVSTRVN
                     TPANNDPSLIEPVPEPDPEPGQGPNPA"
     gene            3229..5541
                     /locus_tag="VNU01_07165"
     CDS             3229..5541
                     /locus_tag="VNU01_07165"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWH32437.1"
                     /translation="MAQSVLGERGTRWFLPSAALLTALIVALPENGLPRHVLHLVLAA
                     ACPVLLLAGVRLFRPRHSRVWYALGAGLALLFAGDLAWRVHALSAGGAPSFIPLADTL
                     SLLGYVWVGVGGVMLLRCRQSGRDRDGLIDAAIFSLVAGLLIWHFVIAPDLRLDAADF
                     GHATSTLLFPLGDVALFALMLRLVLLPGARVRSFWYLLAAMCVAFVCNLATAAFHDVP
                     QVTAGAAGLVLLARAFAAAAALDPSMRSLTEPEPGGARQHHPARLLVLAGCLAVVPTL
                     LGFQDMWGGASNRGLLMAAALTLTTLVLLRLGLVMADRDAAQRQLSHLALHDPLTGLP
                     NRRLLLDRVEMALARSARRPGEVAVLFCDLDRFKVVNDSLGHAAGDELLIEVAARLRQ
                     AVRPDDTVARLGGDEFVICCEGLDGAQGLRAIAERVTRVLGAPVRVAGEEVFVTVSIG
                     LASAQGGPGSAEALIQQADAAMYAAKAAGRDRIESADPEVVDVRSMRLERENALRRAI
                     DKGGLRLEYQPVLDLRTGATAFVEALVRWGHPDGGLLQPDAFIPLAEESGLIVPLGEW
                     VLDEALSQLARWHAAGRNLGCGMSVNVSARQLRDPSFVLHVQRALARTGVAPSALTLE
                     ITERVLLEEAREELASLEALRLLGVQIAVDDFGTRYASLAYLKRLPVNGLKIDRTFVS
                     GVAHDTHDAAIVAAIVTLGHTLGMRVTAEGVETAEQLAAVQALGCDLAQGFYLGAAVP
                     AAEAVAAREVAMSAVPATVSRLAVPVKGLA"
     gene            complement(5641..5716)
                     /locus_tag="VNU01_07170"
     tRNA            complement(5641..5716)
                     /locus_tag="VNU01_07170"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5681..5683),aa:Lys,seq:ctt)
     gene            complement(5763..5836)
                     /locus_tag="VNU01_07175"
     tRNA            complement(5763..5836)
                     /locus_tag="VNU01_07175"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5800..5802),aa:His,seq:gtg)
     gene            5919..5992
                     /locus_tag="VNU01_07180"
     tRNA            5919..5992
                     /locus_tag="VNU01_07180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5953..5955,aa:Arg,seq:tct)
     gene            complement(6004..6345)
                     /locus_tag="VNU01_07185"
     CDS             complement(6004..6345)
                     /locus_tag="VNU01_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HWH32438.1"
                     /translation="MPPPDEIRIRHLVEAAEKAIAYAEHRSREELEEDELLRLALTKL
                     VEIVGEAAKQVSAPTQQMYPQLPWAAAARMRDRLVHHYFDINLDVLWSTVTEDLPGLL
                     AALRESGATDS"
     gene            complement(6332..6667)
                     /locus_tag="VNU01_07190"
     CDS             complement(6332..6667)
                     /locus_tag="VNU01_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWH32439.1"
                     /translation="MTYAMMSSMDIRPGLHVDDEALAQFCRRHAIRRLAVFGSALGND
                     FRDDSDVDILAEFAAGHSPGLMRIAQMELELAELIGGREVEIRTYEDLSRHFRDAVAK
                     QASTLYAAA"
     gene            complement(6808..7107)
                     /locus_tag="VNU01_07195"
     CDS             complement(6808..7107)
                     /locus_tag="VNU01_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWH32440.1"
                     /translation="MATSKLAPVHPGEILLEEFLNPMQLSQYRLAKEISVPPRRINEI
                     VHGKRAVTADTALRLARYFGTTPRFWLNLQAQHDLDSEADRLGDRLEREVVRHAI"
     gene            complement(7118..7399)
                     /locus_tag="VNU01_07200"
     CDS             complement(7118..7399)
                     /locus_tag="VNU01_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWH32441.1"
                     /translation="MIRSFRDRDTQRLAGRERVKKWSPELQKIALRKLRMLDAATEVA
                     DLRVPPGNRLERLKGDRAGQYSIRINDQWRICFRWSSGDAHDVEIVDYH"
     gene            complement(7546..8106)
                     /locus_tag="VNU01_07205"
     CDS             complement(7546..8106)
                     /locus_tag="VNU01_07205"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HWH32442.1"
                     /translation="MNPNALFSAASQIQECCVDHGWAFCFIGGLSVLRWGDPRLTRDV
                     DLTIVTGFGDEEPVIRALTERFPGRIEDAAAFARDNRVLLIQSDNVPIDVTLGALPFE
                     QRATERATPFEIGPDEALRTCSAEDLIVLKAFAGRDRDWADIEGVALRQAGALDEALI
                     WEELLPLLHVKGDEESADRLRRLLAT"
     gene            complement(8103..8324)
                     /locus_tag="VNU01_07210"
     CDS             complement(8103..8324)
                     /locus_tag="VNU01_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32443.1"
                     /translation="MPASGSAERRTVATWQAAGAALRQIRARELQALTDREAVSAFED
                     LYALVAGEPVVREISGLVEQQRLFRRLRS"
BASE COUNT         1234 a         3015 c         3204 g         1373 t
ORIGIN      
        1 cctggaacgc ttccggcaga gcatcgacgt gggcgcgctg cgcgacgggg tcaccacgcc
       61 ggtgccggac gtgcgcggct actccgtgcg gctgttcgcg tcacgccagg aactgggcga
      121 gggcatgggg acgagcttcc cgcagtaccg cgggcgctgg cagccctacg ccgtctgggt
      181 ccccgccggc tacgaggcgg gcacgccgaa tcccctgctg ctgagcctgc acagcctgtc
      241 ggtgcaccac aaccagtacc gcggggggac gagccccagc gcgtcgtaca agacctacta
      301 cgagcagctc gaggccgggc tgggcgccat cgtcgtcacg ccgctggggc gcgggccgga
      361 cggctggtac gaggacgagg gcctggtcga cacgctggag gtctgggccg acgtgcagcg
      421 ccgcttcagc gtcgaccgtg agcgcacgca cgtgggcggc tactcgatgg gcggctacgg
      481 cacctaccgg ctggccacgc tgatgccgga cgcgttcgcg agcgcggtca gcatcgtcgg
      541 cccgccggcc aacggcatct gggcgtaccc gctgccgccc accggcggcg aggcgaaccc
      601 ggacaacacc aggccgcagc tggagaacac ccgccacgtg ccgttctgga tcacccaggg
      661 tgtcctggac gagctggtcc cggtcgcggg cgtggtccat caggcgcgcc gcttcgaaga
      721 gctcggccac gagttccgcc tggcggtgca tccggcggag gaccatctga gcttcgtgtt
      781 caaggacgag tggagccgcg aggtcgcctg gctcgccgcg catccccggc gggtgaccag
      841 cccgcactgc gtcacgctga aggtccggcc cgccagcctg gccaccggcg gccggccgga
      901 gatcctggcg cacgtcgccg cgctgaccgc cgaggtcggt gcccgcgtgg acggcgccta
      961 ctgggtgggc gacgtggcgg tggccgcggg cgccgacgtg accggagtgg tggacctctc
     1021 ctccggggcg gtcacccgcc ggcaggccgg ggtgtcgccg gtcgcgacgg ccggcgtgga
     1081 cgggcccagc ccgcacacgc tgaccgggca ggacaaggtc tgggcggagg gcccgcccgc
     1141 gcagacgctg accggctcgc tgtcgaacgt gacggcgctg acggtggacc tcgaccgcgc
     1201 cggcctggcc ggcgtgccgc aggtggacgt gacgaccgac acgccggtca cgctcacgtt
     1261 cacgtccggc ggcgtgccgg tggggcaggt gagcctggcg ccgtagcggc gctcaggcgc
     1321 tgagcgtctc ccggaccagc tcgcgcaggc ggtcgatgcc gacgttgcgc tcggcgctga
     1381 cccagatgac ctcggagggc gcgacgccgc acgcggcggg caggtcgcgc ttgcgccggt
     1441 cgcgcttgga cgccttcacc ttgtcgtgct tggtcgccac gatctggaac ggcacgccgt
     1501 ggtcgcgcag ccacgcgatc acctcctggt ccagcgacgt gggtccgatc tcgccgtcca
     1561 cgagcagcag gaccaggcgc agcccctccc gctggagcag gtagcgctcg gtgcgccgca
     1621 gccacgcctc gcgcatccgg gcggaggcgg tcgaggcata gccgtagccc ggcaggtcga
     1681 cgaggctggt gccctggggc gtgacgaaga agttgagcag cttggtgctg cccggcttgc
     1741 tggacacggt cgcgagcttg cgcgcggagg ccagcgcgtt gagcagggag gacttgccga
     1801 cgttcgagcg tccggccagg gccacttccg cggccgtcgg cggcaggtcc gccaacctgg
     1861 gcgcggacat cacgaattcg agcttcaggg cgcttgccat gcgcacagtg tcgctggcct
     1921 ggccgggtcg gtgcgcgagg ggggacttga acccccacgg gcgtgagccc accggcacct
     1981 aaagccggcg tgtctgccat tccaccactc gcgcgctgca cgaaaggata ggcccggcgc
     2041 ggcggacggc gccgcgggtc aggcggtcgg caggcggcgc gcggcggcga gcggtgcgac
     2101 cgcggcgttc acgagcaccg ccgtgaacag cgtcgcgccg ctcgccccgg actgcgccag
     2161 gagcacgatc gcggccagcg cgaggagaac cagggcgacc gccacgagcg accgcccacg
     2221 cggcgtgctc gtgtcccgcg gcccggtgtg cagctcgggg gctcgcggtg ccctggtgtg
     2281 ctcggttgcg atcagcatgc aagagggatc gggcgtggcg gggcgccggc gtatcgccgg
     2341 ttcggggggt gtccggacgg ccgggcggtc aggcggggtt gggcccctga ccgggctccg
     2401 ggtccggttc cggcacgggc tcgatgagcg acgggtcgtt gttggccggc gtgttcacgc
     2461 gcgtgctcac cgggtgccac accagcggct cgtccgtcgc ggcggccagg atgcgctcca
     2521 gcccggcagg gtcgcgcagg tcggcgtcca gccacgcgtc ccacagcggc tcgggcagga
     2581 tcaccggcat gcgcgtgtgc agctcgcgga tgcgctcgcc cgcgtcggtc gtcacgatgg
     2641 tgcacgtgcg cagccatccg gcgtcgtccg gcgcgtcctc gggcaggtcg cgctcgcgcc
     2701 acgccgacca cagcccggcg aacgccagcg gcagcccgtc ggcgtggtgg atgaagtacg
     2761 gcaccttgcg cttgtcgccg aggcgcttcc actcgtagaa gccgtcggcg gggatcaggc
     2821 agcgcttgcg cttgaaggcg tcgcggaacg ccggcttctc ggccacggat tccgcacggg
     2881 cgttgatgag cttgttgccg atcttgggat cggaggccca gtgcggcacc aggccccact
     2941 ggaagccgac cagcagccgt tccgcggcgt gtgacgccac cgcgtacacg ggctgggacg
     3001 gcgcgacgtt gtagcggggg ggcagctcgt cggccttgcg ctcgtccacc gtgaagaagc
     3061 gcaccaggcc gtcgggttcg gtgatggaga cgaagcggcc gcacatggcg cccattgtca
     3121 cccagaaatc cggcgctcaa gttccgatcg ggggatgtcg agtagtcaat tcgagctagt
     3181 cctctcttgg acaggacgtc cgagagcgtg cgtgaccaag gatcgggcat ggcgcagagc
     3241 gttctggggg agcgcggaac ccgttggttc ctgccgagtg ccgccctgct caccgccctg
     3301 atcgtcgccc tgccggagaa cggccttccc cgccacgtgc tgcacctggt gctcgccgcc
     3361 gcctgccccg tgctgctgct cgcgggcgtg cggctgttcc ggccccgcca ttcccgcgtc
     3421 tggtacgcgc tcggcgccgg cctggcgctg ctgttcgccg gcgacctcgc ctggcgcgtg
     3481 cacgcgctga gcgccggcgg cgcgccgtcg ttcatcccgc tcgcggacac gctgtcgctg
     3541 ctgggctacg tgtgggtcgg cgtgggcggc gtgatgctgc tgcgctgccg ccagtccggc
     3601 cgcgaccgcg acggcctcat cgacgccgcg atcttctcgc tcgtcgccgg cttgctgatc
     3661 tggcacttcg tgatcgcgcc ggacctgcgc ctggacgccg ccgacttcgg gcacgcgacc
     3721 tcgacgctgc tgttccccct gggcgacgtg gcgctgttcg cgctgatgct gcgcctggtc
     3781 ctgctgcccg gcgcgcgtgt ccgcagcttc tggtacctgc tggccgcgat gtgcgtggcg
     3841 ttcgtgtgca acctggcgac cgcagcgttc cacgacgtgc cgcaggtcac cgccggcgcc
     3901 gccgggctgg tgctgctggc gcgggccttc gcggcggcgg cggccctgga cccgagcatg
     3961 cggtcgctga ccgagccgga gccggggggc gcgcgccagc atcaccccgc gcgcctgctc
     4021 gtcctggccg gctgcctggc cgtggtgccc acgctgctcg gcttccagga catgtggggc
     4081 ggcgcctcca accggggcct gctcatggcc gcggcgctga cgctcaccac gctggtgctg
     4141 ctgcgcctcg gcctggtcat ggcggatcgc gacgccgcgc agcgccagct gtcgcacctg
     4201 gccctgcacg atcccctcac gggcctgccc aaccgccgcc tgctgctgga ccgcgtcgag
     4261 atggcgctgg cgcgcagtgc ccggcgcccc ggcgaggtcg ccgtgctgtt ctgcgacctg
     4321 gaccgcttca aggtcgtcaa cgactcgctc ggccatgccg cgggcgacga gctgctcatc
     4381 gaggtcgcgg cgcggctgcg ccaggcggtg cgcccggacg acacggtggc gcggctgggc
     4441 ggcgacgagt tcgtgatctg ctgcgaggga ctggacgggg cccagggcct gcgcgccatc
     4501 gccgagcgcg tcacgcgcgt gctgggcgcg cccgtgcgcg tggccggcga agaggtgttc
     4561 gtgaccgtgt cgatcggctt ggcgtccgcg cagggcgggc ctggcagcgc cgaggcgctg
     4621 atccagcagg ccgacgcggc gatgtacgcg gccaaggccg cgggcaggga ccgtatcgag
     4681 agtgccgatc cggaggtggt cgatgtacgc agcatgcgac tggagcggga gaacgcgctt
     4741 cgccgtgcca tcgacaaggg cggcctgcgc ctcgagtacc agcctgtcct ggacctccgc
     4801 acgggcgcga cggcgttcgt cgaggcgctc gtccggtggg gtcatccgga cgggggtctg
     4861 cttcagccgg acgccttcat cccgctcgcc gaggagtccg gtctgatcgt ccccctgggg
     4921 gagtgggtac tggacgaggc gctgagccag ctcgcgcgct ggcacgccgc aggccgcaac
     4981 ctgggctgcg ggatgtcggt gaacgtctcc gcgaggcagc tgcgcgaccc cagcttcgtg
     5041 ctgcacgtgc agcgcgcgct ggcgcgcacg ggcgtcgcgc cgtcggcgct gacgctggag
     5101 atcacggagc gggtgctgct ggaggaggcg cgcgaggagc tcgcgtcgct ggaggcgctg
     5161 cggctgctgg gcgtgcagat cgccgtggac gacttcggca cgcgctacgc gagcctggcc
     5221 tatctcaagc gcctgccggt caacggcctg aagatcgacc gcacgttcgt ctccggagtg
     5281 gcccacgaca cgcacgacgc cgcgatcgtg gccgcgatcg tcacgctggg ccacacgctg
     5341 ggcatgcgcg tgaccgccga gggcgtggag acggccgagc agctggcggc cgtgcaggcg
     5401 ctcgggtgcg acctggcgca gggcttctac ctgggggccg ccgtgccggc cgcggaggcg
     5461 gtggcggcgc gcgaggtggc gatgagcgcc gtgccggcga ccgtgtcgcg gctggcggta
     5521 cccgtcaaag gactcgcttg acgcgacaac cctcgcgtca aggcgggact tcgcgtggtg
     5581 atgcgcgagg gcatgggcaa gaaacgaaga agccgcggcg cgtgccgcgg cttcttcgcg
     5641 tggtaggccg cccgggactc gaacccggaa cctgcggatt aagagtccgt tgctctgcca
     5701 attgagctag cgacccgcgc gcgagtctaa cggcgtggta cgaaggccgc atacacgggc
     5761 agggtgaccg acgggaatcg aacccgcgac ctccgcgacc acaacgcggc gctctaaccg
     5821 actgagctac ggtcaccatg cgccgccgca gcggcggttc cgatggtagc cgcgtgcggg
     5881 acagcgcacc aagccgccgc catacttgat gccaccaggc gcccgtagct cagctggaca
     5941 gagcagtcgg cttctatccg ataggtcggg ggttcgaatc ctcccgggcg cgcaacctgc
     6001 ggattaagag tccgtggctc cggattctcg gagggcggcc agcagaccgg gtaggtcctc
     6061 ggtgacggtc gaccacagga catcgaggtt gatgtcgaag tagtggtgca ccaagcggtc
     6121 ccgcattcgg gcagctgccg cccacggtag ctggggatac atctgctgtg tcggcgcgct
     6181 gacctgcttc gctgcctctc cgacaatctc gaccagcttg gtgagggcga ggcggaggag
     6241 ttcgtcttcc tcgagttcct cacgcgagcg gtgctcggcg tatgcgatgg ctttctccgc
     6301 cgcctccacg aggtggcgga tgcggatctc gtcaggcggc ggcatacagg gtgctggcct
     6361 gcttggcgac ggcgtcgcgg aagtggcggc tgaggtcttc gtaggtgcgg atctccacct
     6421 cgcggccgcc gatgagctca gccagttcaa gctccatctg cgcgatgcgc atcaggcctg
     6481 gggaatggcc agcggcgaac tcggccagaa tgtcgacatc gctgtcgtct cggaagtcgt
     6541 tgcccagcgc agacccgaag accgccagcc gccggatcgc atgacggcgg cagaactgcg
     6601 cgagagcttc gtcgtccacg tggagcccag ggcggatgtc catgctgctc atcatcgcgt
     6661 aagtcatccc cgcgcgctcc catctgtgga cgccagcagt tcgtccgccg ccttcgccca
     6721 gtcggggttc gaaagctgtc ctaccagcgg caccttgtga tgtcccgaga catggtgctc
     6781 acctgtctcg ggactcatgc gtgtggatca gatggcgtgg cggacgactt cgcgctccaa
     6841 gcggtcgccg agccgatcag cctcggagtc gagatcgtgc tgggcctgga ggttcaacca
     6901 gaagcggggc gtcgtcccga agtagcgcgc cagccgaagc gccgtatcgg ccgtcacggc
     6961 ccgcttgccg tgaacgatct cgttgatcct gcgtggcggg acgctgatct ccttggccag
     7021 ccggtactga ctgagctgca tcgggttcag gaactcctcc agcaggatct cgccgggatg
     7081 aacgggagcc aacttcgaag tcgccattcc gagcctccta gtggtagtcg acaatctcga
     7141 cgtcatgggc gtctccgctg ctccagcgga aacagatgcg ccactggtca ttgatgcgga
     7201 tgctgtactg gccggctcgg tcgcccttca gtcgttcgag gcggtttccc ggcggaacgc
     7261 ggagatcggc gacttcggtc gcggcgtcca gcatcctcag cttgcgcagg gcgatcttct
     7321 gcagctctgg tgaccacttc ttgactcgct cgcgtccggc cagacgctgg gtatcgcggt
     7381 cgcggaagct ccggatcatg tcgaatcaat cataacgcga cgcgttatca tgcgtcaagc
     7441 gcggtccggc gactgccgga tcttggcgtg catcggggcg acagcaaggg cgctgcccac
     7501 aagtcgacca gggttcgcat ggtgggaggt caggtgcgca gctcgtcagg tggccaagag
     7561 acgccgcaga cgatctgcgc tctcttcgtc gcccttgaca tggaggaggg gcagcaactc
     7621 ctcccagatc aatgcttcat caagcgcgcc ggcctgccgc aacgcgactc cctcgatgtc
     7681 ggcccagtcc cggtcccgcc cagcgaaggc cttgagcacg atcaggtcct ccgcgctgca
     7741 tgtgcgcaga gcctcatccg gcccgatctc gaacggggtc gcgcgttctg tcgcgcgctg
     7801 ttcgaagggg agcgcaccga gcgtcacatc gattggcacg ttgtcgctct ggatgagcag
     7861 gacccggttg tcgcgggcga acgcggccgc atcctcgatc cgcccgggga accgttccgt
     7921 caacgcccgg atgactggct cctcgtcgcc gaatcccgtc acgatcgtca gatcgacatc
     7981 tcgtgtcagg cgaggatctc cccatcgcaa caccgacagg ccaccgatga agcagaacgc
     8041 ccatccgtga tcgacgcagc attcctggat ctggctggcc gccgagaaca atgcgttggg
     8101 attcacgacc ggagacgacg aaacagccgc tgctgctcca cgaggccgga gatctcacgt
     8161 acgaccggct ctcctgccac cagggcatag agatcctcga aggcgctcac ggcttcccgg
     8221 tccgtcagcg cttgcaactc acgggcgcgg atctgccgca atgcagctcc agccgcctgc
     8281 catgtggcaa cggttcgacg ctcggcggag ccagacgcgg gcatgacgcc agcctagcgc
     8341 cagccacgga gcagatgctg gatggacttc acccactcgg ggttcgaaag ctgccctacc
     8401 aggggagcgg tcccgcatcc gggcagccgc cgcccacgga atctgcggat gaagctgctg
     8461 ggtcggcggg tgacctgctt cgcggcctct ccgacgatct tgacccgctt ggtgagcgcc
     8521 aggcggagga gttcgtcctc atgcgtcgtt gcggctccgg gcggcttggg catcgcttcg
     8581 tcagccgggt ccagaaagct gtcctatgag gggcgcaaac cttcggatca aaagtccgcg
     8641 accacgccgg cctgcatggc gtttggcggt cctttccgcc gggtaggcgc ggctcggcga
     8701 atcgccaaag tctgcgtgcg tgcctctggc gggaggtggg agaccggatt ccgcagggct
     8761 gcgcggcatg acgtgttgac cttgccgccg gcgggagcgc gccacggcac gaccgaggag
     8821 gaacac
//
LOCUS       DATMKC010000247         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_6006, whole genome shotgun sequence.
ACCESSION   DATMKC010000247 DATMKC010000000
VERSION     DATMKC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6006"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1046)
                     /locus_tag="VNU01_07215"
     CDS             complement(24..1046)
                     /locus_tag="VNU01_07215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWH32444.1"
                     /translation="MRVVVTGATGNIGTAVMRALSTEPTVDSVLGLARRKPELALPKA
                     EWAEVDIGQAGARQELLSHLRGADAVIHLAWRIQSARDADDMHMVNVAGTEALLSAVA
                     EAGTPALLYASSIGAYAAAPKEPRVDESHATYGIPASLYSLQKAYLERRLDAFAREVP
                     GTRVVRMRPTIVARREVGSEIRRLFLGRVVGRLAGIRPLLPVVPDIPELRFAMVHSDD
                     VAEAFRLALLSDARGAFNLADDEVMGPADLARALHGTTVPMPFAATRAVVSAAWRLHL
                     HPTDAGWLDVAVRLPLVSSERARAELGWRATRSSSEVFGELLEGMREGAGVPTPPLHR
                     AEPPAA"
     gene            complement(1046..>2033)
                     /locus_tag="VNU01_07220"
     CDS             complement(1046..>2033)
                     /locus_tag="VNU01_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWH32445.1"
                     /translation="LGHEPMGRVAEVGPGVTSLKPGDRVVVPFNISCGDCFFCAQGLQ
                     SQCETTQVREHHKGAALFGYTHLYGAVPGGQAEYLRVPMAHYGPVKVPEGPPDERFLY
                     LSDVLPTAWQAVQYAGVPAGGSLTVYGLGPIGLMCVRIARQLGVERVIAVDLVDERLE
                     LGRRWGAETIDYREHDDVAREILDRTKGRGTDSVIDAVGMEAEGSKAAEIAQALKIQM
                     DRLEALRLSIGSVRRGGTLSIVGVYVGVDNLIPMGDLFDKQVQIRMGQANVRRWVDDI
                     LPLLTGDADPLGTEELATHHLPLDAAPAAYDMFQRKQDGCVKVVLHPGEVAA"
BASE COUNT          267 a          785 c          726 g          255 t
ORIGIN      
        1 atgccgggcc aggtcccgca ccgtcaggcc gctggaggtt ctgcccggtg cagcggcggc
       61 gtcggcacgc ccgcgccctc gcgcatgccc tcgagcagct cgccgaagac ctccgacgac
      121 gaccgcgtgg cgcgccagcc gagctccgcg cgggcgcgct cgctggacac cagcggcagg
      181 cgcaccgcga cgtccagcca gccggcgtcg gtggggtgca ggtgcagccg ccaggccgcc
      241 gacaccaccg cgcgcgtggc ggcgaacggc atcggcaccg tggtgccgtg cagcgcccgc
      301 gccaggtccg ccgggcccat cacctcgtcg tcggccaggt tgaacgcgcc gcgcgcgtcc
      361 gacagcagcg ccaggcggaa ggcctcggcg acgtcgtcgc tgtgcaccat ggcgaagcgc
      421 agctcgggga tgtcgggcac caccggcagc agcggccgga tgccggccag gcggcccacc
      481 acacggccga ggaacaggcg gcggatctcc gagccgacct cccgccgcgc gacgatcgtc
      541 gggcgcatgc gcacgacccg ggtgccaggc acctcgcgcg cgaaggcgtc gaggcgccgc
      601 tccaggtagg ccttctgcag cgagtacagc gaggccggga tgccgtaggt ggcgtgggac
      661 tcgtcgacgc gcggctcctt gggcgccgcg gcgtaggcgc cgatggacga ggcgtacagc
      721 agcgccggcg tgccggcctc ggcgaccgcg gacagcagcg cctccgtgcc ggcgacgttc
      781 accatgtgca tgtcgtcggc gtcgcgcgcg ctctggatgc gccaggccag gtggatcacc
      841 gcgtccgcgc cgcgcaggtg cgacagcagc tcctgccgcg ccccggcctg gccgatgtcc
      901 acctcggccc actcggcctt gggcagcgcc agctcgggct tgcgccgcgc caggcccagc
      961 acgctgtcca ccgtgggctc ggtcgacagc gcccgcatca cggccgtgcc gatgttgccg
     1021 gtggccccgg tgacgacgac gcgcatcagg ccgcgacctc ccccgggtgc aggaccacct
     1081 tcacgcagcc gtcctgcttg cgctggaaca tgtcgtaggc ggccggggcg gcgtccagcg
     1141 gcaggtggtg cgtcgcgagc tcctccgtgc cgagcgggtc ggcgtcgccg gtcagcagcg
     1201 gcaggatgtc gtcgacccag cggcgcacgt tggcctgccc catgcggatc tgcacctgct
     1261 tgtcgaacag gtcgcccatg gggatcaggt tgtccacccc cacgtacacg ccgacgatcg
     1321 acagcgtgcc gccgcggcgc accgagccga tcgacaggcg cagcgcctcc agccggtcca
     1381 tctggatctt gagcgcctgg gcgatctcgg ccgccttgga gccctcggcc tccatgccga
     1441 ccgcgtcgat gacgctgtcg gtgccgcggc ccttggtgcg gtcgaggatc tcgcgcgcca
     1501 cgtcgtcgtg ctcgcggtag tcgatggtct ccgcacccca gcgccggccc agctccaggc
     1561 gctcgtccac caggtccacg gcgatgacgc gctccacgcc gagctgccgc gcgatccgca
     1621 cgcacatcag cccgatcggc cccaggccgt acacggtgag cgacccgccc gccggcacgc
     1681 cggcgtactg caccgcctgc cacgccgtcg gcagcacgtc cgacaggtac aggaagcgct
     1741 cgtccggcgg gccctccggc accttcaccg ggccgtagtg cgccatgggg acgcgcaggt
     1801 actccgcctg gccgcccggc acggcgccgt acaggtgggt gtagccgaac agcgccgcgc
     1861 ccttgtggtg ctcgcggacc tgcgtggtct cgcactgcga ctgcagcccc tgcgcgcaga
     1921 agaagcagtc gccgcaggag atgttgaacg gcaccaccac gcggtcgccg ggcttcaggc
     1981 tcgtgacgcc cggcccgacc tcggcgacgc gccccatcgg ctcgtggccg agg
//
LOCUS       DATMKC010000248         9719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_240685, whole genome shotgun sequence.
ACCESSION   DATMKC010000248 DATMKC010000000
VERSION     DATMKC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240685"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /gene="secA"
                     /locus_tag="VNU01_07225"
     CDS             complement(<1..2552)
                     /gene="secA"
                     /locus_tag="VNU01_07225"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWH32446.1"
                     /translation="MVLQKLLRAGEGKRLKQLQRLVGEVNAHEDSVKALSDDALRAKT
                     GEFKGRLDSGETLDDLLPEAFAVAREAAWRVLGQRPYDVQVLGAAALHAGAIAEMRTG
                     EGKTLTSTMAIYLNALNGKGVHVVTANPYLASRDATWMGRIYGFLGLTAGLVHSQQPK
                     ADKRAAYASDITYGTANEFGFDFLRDHMVLTAQDKAQRGHHFCIVDEVDSILVDEART
                     PLIISGPADQATEWYTVFAKRVAPRLQPETHYEVDEAKRTVAVTEEGVAKVEELLGVE
                     NLYESVNTPLIHHLQNAIRAKELFRKDVEYIIEDGEVHIVDEFTGRILYGRRYSEGLH
                     QAIEAKEGVRVKEENQTLATITLQNYFRSYDKLAGMTGTAKTEEAEFSHIYKMDVVVI
                     PTHRPIVRQDHSDLVYKTEKAKYEAAAEDIAQRQAAGQPVLVGTVSVEKSERLANLLT
                     RRGVKHEVLNAKNHFREAEIIAQAGRIGAVTVATNMAGRGVDIMLGGNAESLATVEAG
                     KVADPDEDPEAFQRAYDEHLAHFVEQVKAEGDKVRELGGLYVLGTERHESRRIDNQLR
                     GRSGRQGDPGESRFYLSLEDDLMRLFNASAVESIMTRLKLPEDMPIEHKMVTNAVARA
                     QAQVESRNFELRKNVLKYDDVMNNQRQVVYKQRNMVLEGPDEQIEELAERFIEEAVTG
                     LVEVYAPDGVHPEEWNLEELWTTLETIYPVSLSRDELGIESLKRDELAEMLVEDAFAA
                     YERREEEIGAETLRQVERRVVLSVLDRVWREHLYEMDHLRDGIGLRAVGQRDPLVEYQ
                     REAYEAFIGMMARVKEEAAGYFFNVPVQKVAPEPAPSARPAQADGNGAAPGEA"
     gene            complement(2635..3111)
                     /locus_tag="VNU01_07230"
     CDS             complement(2635..3111)
                     /locus_tag="VNU01_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWH32447.1"
                     /translation="MATWAEFEEAAPDLARFVRGRFEAHTLAFLATLRLDGAPRISGI
                     ETMFAGGHLLLGMMKDSLKTRDLARDPRLALHSASVDKDVKEGDVKLTGRAVAAGPAE
                     FALLAAETGGAHDAGTADLWWVDLESVSSLRIGTPADHLVIESWRPGEAVKTVKRS"
     gene            complement(3122..4099)
                     /locus_tag="VNU01_07235"
     CDS             complement(3122..4099)
                     /locus_tag="VNU01_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32448.1"
                     /translation="MDTWRTLNDLAAAQYGHVSLAQARSAGIPERTLLDTAARQRWAQ
                     PWRGVYRLPGSWPTAMGRLSAALLAVGSPAFVGGWSAAWLVGLVRSAPSAVELVVPHD
                     RRTRNLPGVVVRRSRTLTQADAVHVEGLPSTSAVRTVCDLAAITDEEGLRALLIDGRQ
                     RRLFDLAAVLERASTMGTAKGLSQLRRLVWELDAERCDSVLEYRLRSRLHAVPGLPAP
                     SPAPVPVDVGGRVLHVDIGWPAFAVGIEADGFGSHSERDSLEVDARRHNALERAGWRI
                     LRAGWRSAGPAFDALLRDLVALLGETGAPIAGFRGARTRSNGGGVAPRR"
     gene            complement(4229..4915)
                     /locus_tag="VNU01_07240"
     CDS             complement(4229..4915)
                     /locus_tag="VNU01_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH32449.1"
                     /translation="MTAEETATGGTIRVLVADDHALFRRGLEMVLESEPDIEVVGEAN
                     DGQQAVRLAIEHIPDIVLMDVRMPGGGGIEACRAIKEAAPSVKILMLTISDEEEDLYE
                     AIKAGANGYLLKEISIEEVADAIRSVNGGQSLISPSMASKLLNEFASMAKKEEQKTQV
                     AAPRLTDREMEVLTLVAQGLNNRDIAKELFISENTVKNHVRNILEKLHLHSRMEAVVY
                     AVREKLLEIK"
     gene            complement(5010..5606)
                     /gene="raiA"
                     /locus_tag="VNU01_07245"
     CDS             complement(5010..5606)
                     /gene="raiA"
                     /locus_tag="VNU01_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWH32450.1"
                     /translation="MRVEIVVKSKNCDVPRRVKDEAAQRLAHATRFFDRVLGVEAIFG
                     EETKRRIPEPATVEVTARTKGHRIRAQASAADHAKALDAALERLERQLARYKTRLTDR
                     RRRPGAAPAPMPAPVGPAVADDEAPESGHRIVRSKRFELTPMLPEDAAWHLELLGHDF
                     YVFTNAATGACNVLYRRRDGDLGLIETTAAAPAVAPVG"
     gene            5875..6216
                     /locus_tag="VNU01_07250"
     CDS             5875..6216
                     /locus_tag="VNU01_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32451.1"
                     /translation="MSAVGDPGRARAQDLRAWRRQFGDSCAIGEVEPRRSCVCVGVVR
                     VVRLVPGRSLEITIEDGTGRLTAVFTGRTRLPGLEMGGGLRVRGTVADDDQGRKVMRN
                     PDWSLVSEPYA"
     gene            complement(6207..6887)
                     /locus_tag="VNU01_07255"
     CDS             complement(6207..6887)
                     /locus_tag="VNU01_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWH32452.1"
                     /translation="MRVIVAGAGKIGRYVTLDLVGRGHDVTLVELNPETAKVLAGQVR
                     RLTGDACTPSVLERAGARDADVLVAATGDDEDNLVISLLAKQEFGVPRVLARINHPQN
                     EWMFDEAWGVDVAVSPPHVFTALVEEAVTAGDLVPLLKLEHGQVELLEARLEHRSTAT
                     GRRVEELDLPADSRLVAVVRNGHVLPVRRTTPLAEGDEVLALVRADAKDAFRTGLLGA
                     DGGSEAQA"
     gene            complement(6884..7558)
                     /locus_tag="VNU01_07260"
     CDS             complement(6884..7558)
                     /locus_tag="VNU01_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWH32453.1"
                     /translation="MHVVIGGCGRVGSQLATVLSAQGHDVAVIDKSAAAFRRLGEDFS
                     GQTVQGFCFDRSALEEAGIRRAQAYVAVTSGDNSNVVSARAARDYYGIGKVVARIYDP
                     ERAPIYERLGVRTIASARWTAEQVLRTLLPEGERVDDVEIGPGPGEVVMITLTLPPGV
                     HGLDAAELHEPGAVVLAAITREHATRVPAAGALLEAGDQVHLVVDRGSVERVRERVAR
                     LRGHHG"
     gene            7655..>9719
                     /locus_tag="VNU01_07265"
     CDS             7655..>9719
                     /locus_tag="VNU01_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWH32454.1"
                     /translation="MPRPATALKRALVGRPRTTRELQHTLLPKWMALPVFSSDALSSV
                     AYATQEMMIVLALAGTLAFSHVLPLSLGVALLLVLVVSSYRQTVRAYPQGGGAYIVAR
                     ENLGTAPGLIAGGALLIDYVLTVAVSIAAGMEAITSAVPALIDERVLLSLGFVAVVTV
                     ANLRGVKEAGLLFAIPTYLFITSILALIGLGLAQCVAGCPQAPPTPGIEPNHAGMLTL
                     FLLLRAFASGATALTGVEAISNGVPAFRYPQSHNAAATLGLMGALSVSMFLGISYLAV
                     QTGVQAGEGMTRTVVASIALAVYGGENFGFYVTQVVTALILVLAANTAYADFPRLSSV
                     LAQDRFLPRQFAARGNRLVFSNGIMLLAVAASALLIAFQADVTRLIQLYVVGVFTSFT
                     LSQSGMVRHWLRRRSANWVRSALINGVGGATTAIVLVVVAVTKFTHGAWIVMIAVPAL
                     AVTMHRINRHYFRVGLELRTGMAEPEPARPNHVVVLIDGIDEAAARAVSYAQTLAPAS
                     LEVAAVPAKGSDLQRRWEELGATVPLRVLAPEGTRDPLPAILEHVREKRAAHPGEFVT
                     ALIAETLSHSWWDQLVNHRLALRTKTTLLRERDLVVADLTSPEGGPGPYTLEDPSEHH
                     VVVLVSAVNQATMRALAYAHGLQATSLRALSVSLDAEHAGRMLEAWEDWGVRIPLDIV
                     DSPFRS"
BASE COUNT         1298 a         3522 c         3383 g         1516 t
ORIGIN      
        1 gcctcacccg gggccgcccc gttgccgtcg gcctgggccg gccgcgccga gggcgccggc
       61 tcgggcgcca ccttctgcac cggcacgttg aagaagtagc cggcggcctc ctccttcacg
      121 cgcgccatca tcccgatgaa cgcctcgtac gcctcgcgct ggtactccac cagcgggtcg
      181 cgctggccca ccgcgcgcag gccgatcccg tcgcggaggt ggtccatctc gtacaggtgc
      241 tcgcgccaca cgcggtcgag cacgctcagc accacgcgcc gctccacctg gcgcagcgtc
      301 tcggcaccga tctcctcctc gcggcgctcg taggcggcga aggcgtcctc gaccagcatc
      361 tcggcgagct cgtcgcgctt gagcgactcg atccccagct cgtcgcgcga cagcgacacg
      421 gggtagatcg tctccagggt ggtccacagc tcttcgaggt tccactcctc cgggtgcacc
      481 ccatccggcg cgtacacctc gaccagcccg gtgacggcct cctcgatgaa gcgctcggcc
      541 agctcctcga tctgctcgtc gggaccctcc agcaccatgt tgcgctgctt gtagacgacc
      601 tggcgctggt tgttcatgac gtcgtcgtac ttcagcacgt tcttgcgcag ctcgaagttg
      661 cgggactcca cctgcgcctg tgcgcgcgcg accgcgttgg tgaccatctt gtgctcgatg
      721 ggcatgtcct ccggcagctt cagccgggtc atgatcgact cgaccgcgct ggcgttgaac
      781 agccgcatga ggtcgtcctc gagcgacagg tagaagcgcg actcgcccgg gtcgccctgg
      841 cggccggagc gcccgcgcaa ctggttgtcg atgcgccggg actcgtggcg ctcggtgccc
      901 aggacgtaca ggccgccgag ctcgcgcacc ttgtcgccct cggccttgac ctgctccacg
      961 aagtgcgcga ggtgctcgtc gtaggcgcgc tggaaggcct ccggatcctc gtcggggtcg
     1021 gcgaccttgc cggcctccac cgtggcgagc gactcggcgt tgccgccgag catgatgtcg
     1081 acgccgcggc cggccatgtt cgtggccacg gtgacggcgc cgatgcgccc ggcctgggcg
     1141 atgatctccg cctcgcggaa gtggttcttg gcgttgagca cctcgtgctt gacgccgcgc
     1201 cgggtgagca ggttcgccag gcgctcgctc ttctcgacgc tcacggtgcc gaccagcacc
     1261 ggctgcccgg cggcctggcg ctgcgcgatg tcctcggcgg ccgcctcgta cttggccttc
     1321 tccgtcttgt agacgaggtc cgagtggtcc tggcgcacga tggggcggtg cgtcgggatg
     1381 accaccacgt ccatcttgta gatgtggctg aactcggcct cttcggtctt ggccgtgccg
     1441 gtcatgccgg cgagcttgtc gtaggagcgg aagtagttct gcagcgtgat ggtggccagg
     1501 gtctggttct cctccttgac ccgcacgccc tccttcgcct cgatcgcctg gtgcaggccc
     1561 tccgagtagc gccggccgta gaggatgcgg ccggtgaact cgtcgacgat gtggacctcg
     1621 ccgtcctcga tgatgtactc gacgtccttg cggaacagct ccttggcgcg gatggcgttc
     1681 tgcaagtggt ggatcagcgg ggtgttgacg ctctcgtaga ggttctcgac gcccagcagc
     1741 tcctcgacct tggccacgcc ctcctcggtc accgccacgg tgcgcttggc ctcgtcgacc
     1801 tcgtagtggg tctcgggctg caggcgcggc gcgacgcgct tggcgaacac cgtgtaccac
     1861 tcggtcgcct ggtccgcggg gccggagatg atcagcggcg tgcgcgcctc gtcgaccagg
     1921 atggagtcga cctcgtcgac gatgcagaag tggtggccgc gctgcgcctt gtcctgcgcg
     1981 gtcaggacca tgtggtcgcg caggaagtcg aagccgaact cgttggcggt gccgtaggtg
     2041 atgtcgctgg cgtacgccgc gcgcttgtcg gccttgggct gctgggagtg caccagcccc
     2101 gcggtcaggc ccaggaagcc gtagatgcgg cccatccagg tcgcgtcacg ggaggccagg
     2161 taggggttgg cggtgacgac gtgcacgccc ttgccgttga gcgcgttgag gtagatcgcc
     2221 atggtcgagg tgagcgtctt gccctcaccg gtgcgcatct cggcgatcgc gccggcgtgc
     2281 agggcggcgg cgcccaggac ctgcacgtcg tagggccgct ggcccaggac gcgccacgcg
     2341 gcctcgcgcg cgacggcgaa cgcctccggc agcaggtcgt cgagcgtctc accgctgtcc
     2401 agccgccctt tgaactcgcc ggtcttggcg cgcagggcgt cgtccgacag cgccttgacg
     2461 ctgtcctcgt gcgcgttgac ttcgcccacc aggcgctgca gctgcttgag ccgcttgccc
     2521 tcaccggcac gaaggagttt ctggagcacc acgacgaacg cctctttccc cgactgcgga
     2581 ccgacaaccg aagatccagt gtaggcccag gccgccggct cgcgccgggc gccgtcagga
     2641 gcgcttgacc gtcttgaccg cctcgccggg cctccaggac tcgatgacca ggtggtccgc
     2701 gggtgtgccg atgcgcagcg aggacacgct ctccaggtcc acccaccaca ggtcggccgt
     2761 gccggcgtcg tgcgcgccgc cggtctccgc cgccagcagc gcgaactccg cgggtccggc
     2821 ggccacggca cgtccggtga gcttcacgtc gccctccttg acgtccttgt cgacactggc
     2881 cgagtgcagc gccaggcgcg ggtcgcgggc gaggtcacga gtcttcagcg agtccttcat
     2941 catgccgagc agcaggtgcc cgcccgcgaa catcgtctcg atgccgctga tgcgcggcgc
     3001 cccgtccagt cgcagggtgg ccaggaacgc cagcgtgtgc gcctcgaacc tgccgcggac
     3061 gaaccgggca aggtccggcg ccgcctcttc gaattcagcc caggtagcca tggcaacgac
     3121 gctaccggcg aggtgcgaca ccgcccccgt tcgaacgggt gcgggcaccg cgaaatcccg
     3181 caatgggggc acccgtttcg ccaaggagtg ccacgagatc gcggagcaga gcatcgaacg
     3241 ccggacccgc ggaccgccac cccgcgcgca gaatccgcca cccggcgcgc tcaagggcgt
     3301 tatggcggcg ggcgtccacc tccaggctgt cccgctcgga atgcgagccg aaaccgtccg
     3361 cttcgatacc cacggcgaac gccggccagc cgatgtcgac atgaaggacg cgcccgccca
     3421 catccaccgg gacgggggcc ggagacgggg ccggcagtcc tggcaccgcg tggaggcggg
     3481 agcgaagccg atactccagg accgagtcgc aacgctcggc gtcgagttcc cacacaagcc
     3541 ggcgcaactg agacagcccc ttggcggtgc ccatggtgct cgcccgttcc agcacggccg
     3601 ccagatcgaa gagccgtcgc tgccgaccgt cgatcagcag cgcgcggagc ccctcctcgt
     3661 cggtgatggc cgccaggtca cagaccgtcc ggaccgcact ggtgctcgga agtccctcga
     3721 cgtgcaccgc atccgcctgg gtcagcgtgc ggctgcgtcg caccaccacg ccgggcaggt
     3781 tccgggtccg gcggtcatgc ggcacgacaa gctccacagc cgacggagcc gagcgcacca
     3841 gaccgaccag ccacgcggcg ctccacccac cgacgaaggc gggagagccg acggcgagca
     3901 gcgccgcact cagccggccc atcgccgtcg gccaggagcc gggcaggcgg tagacgccgc
     3961 gccagggctg tgcccagcgc tgacgagccg ccgtgtccag caaggtgcgc tcaggaatgc
     4021 ccgccgatcg agcctgggcc agggagacgt ggccgtactg cgccgcggcc aagtcgttga
     4081 gcgtgcgcca tgtgtccatg cccgcagctt ggacgatgac gcgacgcgcg agccgaaaaa
     4141 cagcttcccc gctgtggaca ccggcgaacg ggtgccccca ctgcgggatg tcgcggtgcc
     4201 cgcacccgtt cgaatgggga agggggggtt acttgatctc gaggagtttt tcgcggacgg
     4261 cgtagaccac ggcctccatg cgggagtgca ggtgcagctt ctccaggatg ttgcgcacgt
     4321 ggttcttgac cgtgttctcg gagatgaaca gttccttggc gatgtcgcgg ttgttgagtc
     4381 cctgcgccac cagcgtcagg acctccatct cgcggtcggt cagccgcggc gcggccacct
     4441 gggtcttctg ctcctccttc ttcgccatcg aggcgaactc gttgaggagc ttggacgcca
     4501 tggaaggaga gatgagcgac tggcccccgt tgacgctgcg gatcgcgtcg gcgacctcct
     4561 cgatggagat ctccttgagc aggtagccgt tggcgccggc cttgatcgcc tcgtagaggt
     4621 cctcctcctc gtcgctgatg gtcagcatga ggatcttgac cgagggcgcc gcctccttga
     4681 tggcgcggca cgcctcgatg ccgccgccgc ccggcatgcg cacgtccatc agcacgatgt
     4741 cggggatgtg ctcgatcgcc aggcgcacgg cctgctggcc gtcgttggcc tcgccgacca
     4801 cctcgatgtc cggctcggac tccagcacca tctccagccc gcgccggaac agtgcgtggt
     4861 cgtcggcgac cagcacccgg atggtgccgc ccgtggcggt ctcctcagcg gtcatcacga
     4921 tctcccacgt ccggctgcga caggcgtgcg cggcccggtc agagtctaac cgggccgctg
     4981 cgcgcaaacc gggtttcgct gcggaagcct cagccgaccg gggcgaccgc cggcgccgcc
     5041 gccgtggtct cgatcagccc gaggtcgccg tcgcggcgcc ggtacagcac gttgcacgcc
     5101 cccgtggcgg cgttggtgaa cacatagaag tcgtggccca gcagctccag gtgccacgcg
     5161 gcgtcctccg ggagcatggg ggtcagctcg aagcgcttgc tgcgcacgat gcggtgcccg
     5221 ctttcgggcg cttcgtcgtc cgccacggcg gggcccaccg gcgccggcat cggcgccgga
     5281 gcggctcccg gacggcgccg gcggtccgtc agccgggtct tgtagcgcgc cagctggcgt
     5341 tcgagccgct ccagcgcggc gtccagggcc ttggcgtggt ccgcggcgct ggcctgcgcg
     5401 cggatgcggt ggcccttggt gcgcgccgtc acctccaccg tcgccggctc cgggatccgg
     5461 cgcttggtct cctcgccgaa gatggcctcc acgcccagga cccggtcgaa gaagcgggtc
     5521 gcatgggcca gccgctgggc cgcctcgtcc ttcacgcggc gggggacgtc acagttcttg
     5581 ctcttcacca cgatctccac acgcacctcc cagatgctcg gtgggatgct gctgggggac
     5641 gcgtctcgcg gcgcggaaca ccgccccgga aacgcgaacg gcctcggacg gatcgtccga
     5701 ggccgcgatt ggcgtcgccg gcgggcgcgg tggcacccgt cacgcatgtt gccttcccgc
     5761 gcatcgcact cctttcggga gcccgtacac gacgccacag gcatcgtctc gcactcgggt
     5821 gcgcagggca agggccatca acgaggtggt ggagcgcggc ctatcatcgc caggatgagt
     5881 gctgtcggag acccgggccg cgcccgcgcg caggacctgc gcgcctggcg ccgccagttc
     5941 ggcgactcct gcgcgatcgg cgaggtggag ccgcgccggt cctgcgtctg cgtgggcgtc
     6001 gtgcgcgtcg tgcggctggt gccgggccgg tcgctggaga tcaccatcga ggacggcacg
     6061 ggccgcctga ccgccgtgtt caccggccgc acccggttgc ccggcctgga gatgggcggg
     6121 ggcctgcggg tccgcggcac ggtcgccgac gacgaccagg gccggaaggt gatgcgcaac
     6181 cccgactgga gcctggtgag cgagccctac gcctgagcct cgctgccgcc gtcggcgccc
     6241 agcaggcccg tccggaacgc gtccttggcg tcggcgcgca ccagcgccag cacctcgtcg
     6301 ccctccgcca gcggcgtggt gcgccgcacg ggcagcacgt gcccgttgcg cacgacggcg
     6361 accaggcggc tgtcggcggg caggtccagc tcctccacgc gcctgccggt ggccgtggag
     6421 cgatgctcca ggcgcgcctc gagcaactcc acctggccgt gctccagctt cagcagcggc
     6481 accaggtcgc cggcggtgac ggcctcctcc accagggcgg tgaacacgtg cggcgggctg
     6541 accgccacgt ccacgcccca ggcctcgtcg aacatccact cgttctgcgg gtggttgatg
     6601 cgtgccagca cgcgcgggac gccgaactcc tgcttggcca gcagcgagat cacgaggttg
     6661 tcctcgtcgt cgcccgtggc ggccaccagc acgtcggcgt cgcgcgcgcc cgcgcgctcc
     6721 agcaccgatg gcgtgcacgc gtccccggtg aggcggcgca cctggccggc cagcaccttg
     6781 gcggtctccg ggttgagctc gaccagcgtg acgtcgtgcc cgcggccgac caggtccagc
     6841 gtgacgtagc ggccgatctt gccggccccg gcgacgatga ctctcatccg tgatgccccc
     6901 tcaggcgggc gacgcgctcg cgtacccgct ccaccgaccc gcggtcgacc acgaggtgca
     6961 cctggtcgcc ggcctccagc agcgccccgg cggccggcac gcgcgtggcg tgctcgcgcg
     7021 tgatggcggc gagcaccacc gcgcccggct cgtgcagctc cgcggcgtcc agcccgtgca
     7081 cgcccggcgg cagcgtgagc gtgatcatca ccacctcgcc gggccccggg ccgatctcca
     7141 cgtcgtcgac gcgctcgccc tccggcagca gcgtgcgcag gacctgctcg gcggtccagc
     7201 gcgcggaggc gatggtgcgc acacccaggc gctcgtagat cggcgcccgc tccgggtcgt
     7261 agatgcgggc gaccaccttg ccgatcccgt agtagtcgcg cgcggcgcgc gcggacacga
     7321 cgttggagtt gtcgccgctg gtgacggcga cgtaggcctg cgcgcggcgg atgccggcct
     7381 cctccagcgc cgagcgatcg aagcagaagc cctgcacggt ctggccggag aagtcctcgc
     7441 cgaggcggcg gaacgcggca gcgctcttgt cgatcaccgc gacgtcgtgg ccctgcgcgg
     7501 acaggaccgt cgcgagctgg ctgccgacgc gtccgcagcc tccgatcacc acatgcaccc
     7561 tgggttttcc tcctgatcga ccaaccgacc taccataccc ccggcgcggg agcgccccgc
     7621 acgggcaggg caacacccca cgacaggagg agcgatgccg cgtcccgcga ccgcgttgaa
     7681 gcgcgcgctg gtcggacgcc cgcgcaccac cagggagctg cagcacacgc tgctgccgaa
     7741 gtggatggcg ctgccggtgt tctcctccga cgcgctgagt tcggtggcct acgccaccca
     7801 ggagatgatg atcgtcctgg cgctggcggg gacgctggcg ttctcgcatg tgctgccgct
     7861 gtcgcttggc gtggcactcc tgctcgtcct ggtggtgagc agttaccgcc agacggtgcg
     7921 cgcctacccg cagggcggcg gcgcgtacat cgtcgcgcgc gagaacctgg gcacggcgcc
     7981 ggggctgatc gccggcggcg ccctgctgat cgactacgtg ctcacggtgg ccgtgtccat
     8041 cgccgcgggc atggaggcga tcacgtcggc ggtgccggcg ctcatcgacg agcgggtgct
     8101 gttgtcgctg ggcttcgtcg ccgtcgtcac ggtggcgaac ttgcgcggcg tcaaggaagc
     8161 cggactcctg ttcgcgatcc ccacgtatct gttcatcacg tcgatcctgg cgctgatcgg
     8221 actgggcttg gcgcagtgtg tcgccggctg cccgcaggcc ccgccaacgc ccggcatcga
     8281 gccgaaccac gccgggatgc tcacgctgtt cctgctgttg cgcgcgttcg cgtccggcgc
     8341 gaccgcgctc accggcgtcg aggccatctc caacggggtg ccggccttcc gttacccgca
     8401 gagccacaac gccgccgcca cgctcggcct catgggcgcc ctgtccgtgt cgatgttcct
     8461 gggaatcagc tatctggcgg tgcagaccgg cgtgcaggca ggggagggca tgacccgcac
     8521 cgtggtcgcc tccatcgccc tggcggtcta cgggggggag aacttcggct tctacgtgac
     8581 ccaggtcgtc acggcgctga tcctcgtcct ggcggccaat accgcctacg cggactttcc
     8641 gcgcctgtcc tccgtgcttg cccaggaccg atttctgccg cggcaattcg ccgcccgcgg
     8701 gaaccgcctg gtgttctcca acggcatcat gctcctggcg gtggcggcct cggcactgct
     8761 gatcgccttc caggccgacg tcactcggct gatccagctc tacgtggtcg gcgtgttcac
     8821 gtcgttcacc ctgagccaga gcggcatggt gcgccactgg ctgcgccggc gcagcgccaa
     8881 ctgggtgcgc tcggcgctga tcaacggggt cgggggcgcc accacggcga tcgtcctggt
     8941 ggtcgtggcg gtgaccaagt tcacccacgg ggcgtggatc gtgatgatcg ccgtgccggc
     9001 cctggccgtc accatgcacc gcatcaaccg gcactacttc cgcgtgggcc tggagctgcg
     9061 caccggcatg gccgagcccg agccggcccg gcccaaccac gtggtggtgc tgatcgacgg
     9121 catcgacgag gcggccgccc gcgccgtgtc ctacgcgcag acgctggcgc ccgcgagcct
     9181 ggaggtcgcg gccgtgccgg ccaagggctc cgacctgcag cggcgctggg aggagctcgg
     9241 cgccaccgtg ccgctgcgcg tgctggcgcc ggagggcacg cgggacccgc tgccggccat
     9301 cctcgagcac gtgcgcgaga agcgggcggc gcaccccggc gagttcgtca cggcgctgat
     9361 cgccgagacg ctgtcgcaca gctggtggga ccagctcgtc aaccaccggc tggccctgcg
     9421 caccaagacg acgctgctgc gcgagcggga cctggtcgtg gcggacctca cctctcccga
     9481 gggcgggccc ggcccctaca cgctggagga ccccagcgag caccacgtgg tggtgctggt
     9541 gtcggccgtg aaccaggcga cgatgcgcgc cctggcctac gcccacgggc tgcaggcgac
     9601 gtcgctgcgc gcgctgtcgg tgagcctcga cgccgagcac gccgggcgga tgctggaggc
     9661 gtgggaggac tggggcgtgc ggatcccgct ggacatcgtc gacagcccgt tccgcagca
//
LOCUS       DATMKC010000249        13952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_24304, whole genome shotgun sequence.
ACCESSION   DATMKC010000249 DATMKC010000000
VERSION     DATMKC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24304"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VNU01_07270"
     CDS             complement(<1..789)
                     /locus_tag="VNU01_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWH32455.1"
                     /translation="MSGAGLTRANVTRLVVLACIWGASFLLIKFALEGLSPIQIVLAR
                     LSFGTLALVAILAVRRIALPRGGRMWGHLALMAVVANVIPFFLFGWGEQRITSGMAGV
                     LNGTTPLFTLVFASAALPEERLSLGRALGLLIGFTGVVLVVGPWDQSGANALSGQLAI
                     VLAAACYGVAFTYTRRFISGRGNPLALAAGQLGAGAVLLWVAAPLVAADPVSLTTRVV
                     LSAGALGAVGTGLAYMLYYRLITDAGATTASMVTYLIPVVAVILG"
     gene            complement(786..1523)
                     /locus_tag="VNU01_07275"
     CDS             complement(786..1523)
                     /locus_tag="VNU01_07275"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32456.1"
                     /translation="MSDVITTRLRVPLRRPQAVPEVSALLWEPGRPVDGAAGLVLAHG
                     AGTDMTSPLLRAVARGLAEQGHPVLLFNFSYTEAGRKAPDPMPRLEACSREVAAVARE
                     RFGGRPLVLGGRSMGGRVASHLAAQGEPCDGLVFLGYPLHPPRRAGAAVPDDRLRTAH
                     WPALRTPALFVQGDRDTLADLALLERERAARLAGPSQVHVVRGGDHGFAVRKRDGRDP
                     ADVLAEIVGVVAAWVSGLSPAAAEAGA"
     gene            1679..2254
                     /locus_tag="VNU01_07280"
     CDS             1679..2254
                     /locus_tag="VNU01_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWH32457.1"
                     /translation="MYAGFTFLQVWHASEQDEVHPAGAIVVLGAAQYDGLPSPALAAR
                     LDRAAALYAQGVAPIVVATGGQRPGDRYTEANAGGRYLLAHGVPDSALRLEVQGRSTW
                     ESLAATARFLRREGVREVLLVTDAYHAFRVAEVAREVGLVPHVTPVNPGKASLRSLGR
                     ETVAVAVGRFLGYRRLTRLDQVIEQASEIVR"
     gene            2251..3486
                     /locus_tag="VNU01_07285"
     CDS             2251..3486
                     /locus_tag="VNU01_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWH32458.1"
                     /translation="MTELARRLGLRDAVVIGLGAMLGAGVFAAPGPAAAAAGSWLLAG
                     LALAAAVAYANATSSAQLAARYPESGGTYVYARERLGPYWGFLAGWGFVVGKSASLAA
                     MALTFGAYVHPPLARPLGVAAVVVLAAVNARGVDKTVRLTRVIVAAVLAVLGLAVAAA
                     LGGGTASPANLSAGLPAGAPGVLQAGGLLFFAFAGYARLATLGEEVREPQVTIPRAIP
                     IALGIVVAVYAVVLGAALLAIGPERLAASPAPLAAAVEAGRWAAAAPVVRAGAAVAAA
                     GVLLSLLAGVSRTSLAMARRRELPGVLDAVHPRHRTPHRAEFAAATVVCAVVAVADLR
                     GAIGFSSFAVLAYYALANASAWTLPAAQRRWPRGLAAFGVAGCAVLALALPRASVAAG
                     VTVLGAGSLAWLTTRRRRR"
     gene            complement(3492..4700)
                     /locus_tag="VNU01_07290"
     CDS             complement(3492..4700)
                     /locus_tag="VNU01_07290"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWH32459.1"
                     /translation="MSDLPAAAERLDGDPPLHELLERDADDLYDHAPCGYLSALPDGT
                     LVKVNTTFLRWTGLRREDLVGRRRFQELLSPGGQIYHETHYAPMLRMQGSVREIAVDI
                     VRADGSRMPALVNSTLKQDADGNPLLVRTTVFDATERREYERELLRARQRAEESEARA
                     RVLAQTLQQSLIPPAPPAVPGIDVGAVYRPALSGEEVGGDFYDVFELPAGDWAVVIGD
                     VCGKGAQAAAVTALARHTVRAAAIRESRPEAVLSLLNQALLRQDAPRFCTVAYARLAR
                     RPGGDVEVHFASGGHPLPILVTGTMRPRSAGRPGDLLGVMPEATAEASTLVLEPGDAL
                     VFFTDGITEARRGEEFYGDERLAAALATRREAAAQPMAQGLVDEVLAFSGGLPRDDIA
                     VVAVRAAPAA"
     gene            complement(4693..5505)
                     /locus_tag="VNU01_07295"
     CDS             complement(4693..5505)
                     /locus_tag="VNU01_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH32460.1"
                     /translation="MGAFGRNNVTVSGREDGRPMLFVHGFGCDQQMWRYVTPEFSDTH
                     RVVLMDHVGHGGSDHAAYDPARYATLDGYAADLVEILEELDLRDVVIVGHSVSAMIGA
                     LAAIRAPQRVGALVMVSPSPRYLDDDGYVGGFQREAIEELLDSLDANYLGWSSAMAPV
                     IMGNAERPELGQELTSSFCRTNPDIARDFARVTFLSDNRADLARVPVPTLVLQCAEDV
                     LAPDAVGQYVADQVADATLVRLRATGHCPNLSAPEETVAAIKAFLPRVGAGV"
     gene            5630..6601
                     /locus_tag="VNU01_07300"
     CDS             5630..6601
                     /locus_tag="VNU01_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWH32461.1"
                     /translation="MTRVGLVLGAGGTVGQAYHAGVLAALEHDLGWDPRSADVIVGTS
                     AGSITGALLRLGVPAAELAAWAVQAPLRLDGGAVEDWLSRERPQFPPFTPRHWLRPWR
                     PPSPALVAKMARRPWSVRPAVAALTCAPRGRVDLLVHAGALEELAGEWPEGLRVCAVR
                     RHDGERVAFGAEGAPAAALATAVASSCAIPGYFAPVRIGGREYVDGGVHSPSNADALA
                     GDELDVVIAVSPMSTSTRVRALDAPVRYAAHRRLVRELRRLERRGVTVVRFEPGGRSL
                     AAMGINMMADDRADAVVQAAFIEAGGMAAGRLLADRLAPVRARAAAQ"
     gene            6669..7847
                     /locus_tag="VNU01_07305"
     CDS             6669..7847
                     /locus_tag="VNU01_07305"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF005568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWH32462.1"
                     /translation="MALAPLLPPLQGFTVGITAARRWEDQAQHLASRGAEILHAPALR
                     SDPADPEGELRHVTEALVTRPPDLTILTTGAGASAWFSAADSFGAGSELLEALRRGAV
                     IPRGPKTVAACIQAGLDVPRRGGHERVREVLEAARAGGGLEGRRVAVQLDGAATSWLV
                     DELRREGAQVVSVRVYHWQPSHDDEPVRKLIDGVCRLRVHAVTFTSTPAVRNVFSVAR
                     QAGRDAELRERFASGTAAVSIGPVTSEALRREGVADPVEPARARLGAMVAALVHHLEG
                     RRRILVLAGCEVLIQGAAVLVDGAPRALSEQDRHLLAVLAEANGRVVPKQALLDRGLG
                     AGAVSEHAVEVAVARLRRRLGPAGAGVQTVFRRGYRLALDGAGSGSATSITPPVRRTS
                     "
     gene            complement(7772..8128)
                     /gene="nirD"
                     /locus_tag="VNU01_07310"
     CDS             complement(7772..8128)
                     /gene="nirD"
                     /locus_tag="VNU01_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HWH32463.1"
                     /translation="MSGVWIPLCALDDIVPDSGVCARVGGRQVAVFRLRDGDVHAVGN
                     VDPFSGAAVLSRGILGDVAGVPVVASPVYKERFDLRTGTCLDDPAVRIPVYDVRLTGG
                     VIEVAEPEPAPSSASR"
     gene            complement(8125..10707)
                     /gene="nirB"
                     /locus_tag="VNU01_07315"
     CDS             complement(8125..10707)
                     /gene="nirB"
                     /locus_tag="VNU01_07315"
                     /inference="COORDINATES: protein motif:HMM:NF011565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HWH32464.1"
                     /translation="MNPTLVVAGNGMVGQKLLDCLVEAGGADAWRVVVFGEEPRAAYD
                     RVNLSTYFDGRSAEDLSLLSPEVAERVELVLGDPVTAVDRAARTVATASGSRERYDAL
                     VLATGSAPFVPPIEGARGPGCFVYRTLDDLDAIRAAAAGARVGVVLGGGLLGLEAANA
                     LRHLGLETHVVEMAPRLMPVQVDDGGGAILRQEIEALGIGVRTGAVTERIVHGPGGAR
                     LEFSDGDALDADVVVISAGIRPRDELARACGLDVAERGGVVVDEACRTSDPAIFAIGE
                     CASFGGRVYGLVAPGYAMARVVAGRLLGGDAVFAPADMSTKLKLLGVDVASFGDAHGT
                     AENARSLVWSDPMARVYRRLVVDGEGTRVLGGILVGDAADYAALHQRCLSALPLPGDP
                     ADLVLPARAGGGAALGPAAIPMESTVCNCNNVSKAAICAAIAGEGLTTVGEVKACTAA
                     GTGCGSCVPLVKGILEHELERAGVAVDRSVCEHFPHTRQELADIVRVREVRTFAELLD
                     GWGTGRGCDVCKPAVASILASFWNEYVLADEHLPLQDTNDRFLANLQKDGTYSVVPRV
                     PGGELTPAQLIVLGEVARDHDLYTKITGGQRVDLFGARLDELPAIWARLVAAGFESGH
                     AYGKALRTVKSCVGESWCRYGVQDSTSLAIDLELRYRGLRAPHKIKCAVSGCARECAE
                     AQGKDVGVIATEKGWNLYVCGNGGMRPQHAVLLAEDLDTETLVRYVDRFLMFYVRSAD
                     RLERTATWLNRLEGGVDYLRRVVIDDGLGICEQLEADMARHVATYRCEWKATLEDPVR
                     LRAFRHFVNDETPDPTLAYVRERGQRRPARPEEREAVDMAGVALPQADAVTGLAGAVT
                     GGRA"
     gene            10870..12369
                     /locus_tag="VNU01_07320"
     CDS             10870..12369
                     /locus_tag="VNU01_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HWH32465.1"
                     /translation="MTTSHDAATRLLALRSFTGRYRVLHLTWLAFFLTFVVWFNFAPF
                     ASTVSAAFGLSGEQVAVLALCNVALTVPARVLVGMALDRWGPRRVYAAILMYAAVPCL
                     AFAAATSFGMLVASRLALSVVGAGFVVGIRMVAEWFPPREVGMAEGVYGGWGNFGSAA
                     AALALPTLAAVLGGADGWRWAIGSTGIVSAAYGAWYLRAVRDTPDGLPLVRPRRQGAL
                     EVTHPGAVAGLLALNLPLLGALGLVAWRLARVGFLSGAGLTAVVVTLGALAVLQARTV
                     WRVNAPARQDAYDPGDRYPFRSVVVLCLAYAVTFGSELAVVSMLPTFFAGTFGLSTVA
                     AGATASAFAFMNLGARPAGGLLSDLLGSRRRTLTAMLLGLSGGYALMAAVGSGWPVPL
                     AMVAAMTCSLFVQAGEGATYAIVPLVKRRVSGQIAGLVGAYGNVGALAYLTLLLFAGP
                     TVFFLAISASAIAAAIACRWLVEPQGSFAHVHEPSDAVTAAPALEERIA"
     gene            12366..>13952
                     /locus_tag="VNU01_07325"
     CDS             12366..>13952
                     /locus_tag="VNU01_07325"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWH32466.1"
                     /translation="MTDLLTPLDRYLAEQRGTTAVGRFARWHERDGGGRLAALTHYRE
                     LVPLERPGPGEQYAFQVDLDSCTGCKACVSACNHMNGLDEGESWRSVGLLVGSRGDQP
                     FQQSVTAGCHHCAEPACLSGCPVDAYEKDAETGIVHHLDDQCIGCRYCILMCPYEVPT
                     FSDRLGIVRKCDMCSGRLAAGEAPACAQACPNGAITITVVARAESAARATGGEATVPG
                     APDPRITLPTTVYRTTRPALLASEAADRSSAEPAHDHPPLVVMLVLTQLSVGAFATDY
                     LLTVLDRRVVAGPFPAAFALAAGLLALGASLLHLGRPRYAYRAVVGLRHSWLSREIVA
                     FGGFAGVAAAYAAARWLTPGTMTPPMLALGALVVLTGAAGVGCSVMIYAVTRRTWWRA
                     AMTAPRFALSGAVSGASVVMAAGVVTAVLGGATALSAPTGPLALTVMGATAAKLALEA
                     EVLRNRRAGSPADLAGTARLLAGPLRGLVQRRVAAGMAGGIVVPGILALLATEPEAPV
                     GVAAGFAVTGALLVLAGELWE"
BASE COUNT         1621 a         5218 c         5325 g         1788 t
ORIGIN      
        1 gcccaggatc acggcgacca cggggatgag gtaggtcacc atcgacgccg tggtggcgcc
       61 cgcgtcggtg atgagccggt agtacagcat gtaggccagg ccggtcccga cggcgcccag
      121 cgcaccggcg ctcagcacca cccgcgtggt cagcgacacc gggtccgcgg cgaccagcgg
      181 cgcggccacc cacagcagca ccgcgccggc gccgagctgc ccggccgcca gcgcgagcgg
      241 gttgccacgc ccggagatga aacgccgggt gtaggtgaag gcgacgccgt agcacgccgc
      301 ggcgagcacg atcgcgagct ggcccgacag cgcgttcgcg ccgctttggt cccacggccc
      361 cacgaccagg accacgccgg tgaacccgat cagcagcccg agcgcgcggc cgagcgacag
      421 gcgctcctcc ggcagcgcgg ccgaggcgaa caccagcgtg aacagcggcg tggtgccgtt
      481 cagcaccccg gccatgccgg aggtgatgcg ctgctccccc cagccgaaca ggaagaacgg
      541 gatgacgttg gccaccacgg ccatgagcgc cagatggccc cacatccggc cgccgcgcgg
      601 cagcgcgatc cgccgtacag ccaggatggc cacgagcgcc agcgtgccga acgacaggcg
      661 cgccagcacg atctggatgg gcgacaggcc ctccagcgcg aacttgatca gcaggaagct
      721 cgcgccccag atgcaggcca gcaccaccag gcgcgtcacg ttggcgcgcg tcagcccggc
      781 accgctcacg cgcccgcctc cgccgcggcg ggcgacaggc cggacaccca cgcggccacc
      841 acgccgacga tctcggcgag cacgtccgcg gggtcgcggc cgtcccgctt gcgcaccgcg
      901 aagccgtggt cgccgccgcg caccacgtgg acctggctcg gcccggccag gcgcgcggcg
      961 cgctcgcgct ccagcagcgc caggtcggcc agcgtgtcgc ggtcgccctg cacgaagagt
     1021 gccggcgtgc gcagcgccgg ccagtgcgcg gtgcgcagcc ggtcgtccgg cacggccgcg
     1081 ccggcgcgcc gcggcgggtg cagcgggtag cccaggaaca ccaggccgtc gcacggctcg
     1141 ccctgcgccg ccaggtgcga cgccacccga ccgcccatcg accgcccgcc caggaccagc
     1201 gggcgcccgc cgaagcgctc gcgcgccacc gcggcgacct cgcgcgagca ggcttccagt
     1261 cgtggcatcg ggtcgggggc cttccgtccg gcttcggtgt aggagaagtt gaacagcagg
     1321 accggatgcc cctgttccgc caggccccgg gccactgccc gcaaaagtgg actggtcatg
     1381 tccgtgccgg cgccgtgcgc cagcaccagg ccggccgccc cgtccaccgg ccggcccggc
     1441 tcccacagca gggcggagac ctccggcacg gcctgggggc ggcgcagcgg gacgcgcagg
     1501 cgcgtggtga tgacatcgga cacgggaaac ggactttctg gacggggggc gagcagcgga
     1561 tggtacccgg cacgccgcgt agcctgggac gcgccaggcc ccggcgaaag gagcacggtt
     1621 gcgcaggcca gggcggctcg ccggacggct cgccgctgtg atcaccgcgt tgagcgcggt
     1681 gtacgccggc ttcacgttcc tgcaggtgtg gcacgcctcg gaacaggacg aggtccaccc
     1741 ggcgggcgcg atcgtcgtcc tgggcgcggc ccagtacgac ggcctgccct cgcccgcgct
     1801 ggcggcgcgg ctggaccgcg ccgcggcgct gtacgcgcag ggcgtggcgc ccatcgtggt
     1861 ggccacgggc gggcagcggc ccggcgaccg ctacaccgag gcgaacgccg gcgggcgcta
     1921 cctcctcgcc cacggcgtgc cggacagcgc cctgcgcctg gaggtgcagg gccgcagcac
     1981 gtgggagtcg ctggccgcga ccgcgcgctt cctgcgccgc gagggcgtcc gcgaggtgct
     2041 cctggtgacc gacgcctatc acgcgttccg ggtcgcggag gtggcgcgcg aggtggggct
     2101 ggtgccccac gtcaccccgg tgaacccggg caaggcgtcg ctgcgcagcc tggggcgcga
     2161 gacggtcgcc gtcgcggtgg ggcgcttcct gggctaccgg cggctcacgc gactggacca
     2221 ggtcatcgag caggccagcg agatcgtgcg gtgaccgagc tcgcccggcg cctcggcctg
     2281 cgcgacgccg tggtgatcgg gctgggcgcg atgctgggcg ccggagtgtt cgccgcgccg
     2341 ggaccggcgg cagccgccgc ggggagctgg ctgctggccg ggctcgccct ggccgcggcc
     2401 gtcgcgtacg ccaacgcgac ctcgtcggcg cagctcgccg cgcgctaccc ggagtcgggc
     2461 gggacctacg tgtacgcgcg cgagcgcctg ggcccgtact ggggcttcct ggcgggctgg
     2521 ggcttcgtgg tcggcaagag cgcgagcctc gcggccatgg cgctgacgtt cggcgcctac
     2581 gtgcacccgc cgctggcccg tccgctcggc gtcgccgccg tggtggtgct ggccgccgtg
     2641 aacgccaggg gcgtggacaa gaccgtgcgc ctgacgcggg tgatcgtggc ggccgtgctg
     2701 gccgtgctgg gcctggccgt ggccgccgcg ctgggcggcg gcaccgccag cccggccaac
     2761 ctcagcgccg ggctgcccgc cggcgcgccc ggcgtgctgc aggccggcgg gctgctgttc
     2821 ttcgccttcg ccggctacgc gcgcctggcc accctcggcg aggaggtgcg cgagccgcag
     2881 gtgacgatcc cgcgggcgat ccccatcgcg ctgggcatcg tggtcgccgt gtacgcggtg
     2941 gtgctcggcg cggcgctgct ggccatcggc cccgagcggc tggcggcgtc gcccgcgccg
     3001 ctggcggccg ccgtggaggc ggggcgctgg gcggccgcgg cgccggtggt gcgggcgggc
     3061 gccgcggtgg ccgccgcggg cgtgctgctg tcgctgctgg cgggcgtgag ccgcaccagc
     3121 ctggcgatgg cccgccgccg cgagctgccg ggcgtgctgg acgccgtcca cccgcggcac
     3181 cggacacccc accgcgccga gttcgccgcg gccaccgtgg tgtgcgcggt cgtcgccgtg
     3241 gccgacctgc gcggggcgat cgggttcagc tccttcgccg tgctggccta ctacgcgctg
     3301 gcgaacgcct cggcgtggac gctgccggcg gcgcagcggc gctggccgcg cggcctggcc
     3361 gcgttcggcg tcgccggctg cgccgtgctc gccttggcgc tgccgcgcgc ctccgtggcg
     3421 gcgggcgtga ccgtcctggg cgccgggtcg ctggcgtggc tgacgacgcg gcggcgccgg
     3481 cggtagcggg ctcaggcggc tggtgcggca cgcacggcga ccaccgcgat gtcgtcgcgc
     3541 ggcaggccgc cggagaaggc caggacctcg tccaccaggc cctgcgccat cggctgggcg
     3601 gcggcctcgc gccgggtcgc cagggcggcc gccaggcgct cgtcgccgta gaactcctcg
     3661 cctcggcgcg cctcggtgat gccgtcggtg aagaacacca gcgcgtcgcc cggctccagc
     3721 accagcgtgc tggcctccgc ggtggcctcc ggcatgacgc ccagcaggtc gccgggccgc
     3781 cccgcggacc ggggccgcat ggtgccggtc accaggatgg gcagcgggtg gccgccggag
     3841 gcgaagtgca cctccacgtc gccgccgggc cggcgcgcca gccgcgcgta cgcgaccgtg
     3901 cagaagcgcg gcgcgtcctg gcgcagcagc gcctggttca gcaggctcag caccgcctcc
     3961 gggcggctct cccggatcgc cgccgcccgc acggtgtggc gcgccagcgc cgtcaccgcc
     4021 gcggcctgcg cgcccttgcc gcagacgtcg ccgatcacca cggcccagtc tcccgccggc
     4081 agctcgaaca cgtcgtagaa gtcgccgccg acttcctccc cggacagcgc cgggcggtag
     4141 acggcgccca cgtcgatgcc gggcaccgcc ggcggcgccg gcgggatgag gctctgctgc
     4201 aaggtctgtg ccagcacccg ggcgcgcgcc tcggactcct cggcgcgctg gcgcgcgcgc
     4261 agcaactcgc gctcgtactc gcggcgctcg gtcgcgtcga agacggtggt gcgcaccagc
     4321 agcgggttgc cgtcggcgtc ctgcttgagc gtggagttga ccagcgccgg catgcggctg
     4381 ccgtcggcgc gcacgatgtc cacggctatc tcgcgcacgg agccctgcat gcgcagcatc
     4441 ggcgcgtagt gggtctcgtg gtagatctgc ccgcccggcg acagcagctc ctggaagcgc
     4501 cggcggccga ccaggtcctc gcggcgcagt cccgtccagc gcaggaacgt ggtgttgacc
     4561 ttgacgaggg tgccgtcggg cagggccgac aggtagccgc agggggcgtg gtcgtacagg
     4621 tcgtcggcgt cgcgctccag cagttcgtgc aggggcgggt cgccgtcgag ccgctccgcg
     4681 gccgccggga ggtcagacac cggcgcccac gcgcggcagg aacgccttga tggccgccac
     4741 ggtctcctcc ggcgcgctca ggttggggca gtggccggtg gcgcgcaagc gcacgagcgt
     4801 cgcgtcggcg acctggtcgg cgacgtactg ccccacggcg tccggcgcga gcacgtcctc
     4861 ggcgcactgc agcaccaggg tcggcacggg gacgcgcgcc aggtcggcgc ggttgtccga
     4921 aaggaacgtc acgcgcgcga agtcccgggc gatgtcgggg ttggtgcggc agaagctgga
     4981 ggtcagttcc tgccccagct cgggacgctc ggcgttgccc atgatcacgg gcgccatcgc
     5041 actggaccag cccaggtagt tggcgtccag cgagtccagc agctcctcga tcgcctcgcg
     5101 ctggaagccg ccgacgtagc cgtcgtcgtc caggtagcgc ggcgacggac tgaccatgac
     5161 cagggcgccg acgcgctggg gggcgcggat ggccgccagc gcgccgatca tcgcgctcac
     5221 ggagtggccg acgatcacca cgtcgcgcag gtccagctcc tccaggatct ccacgaggtc
     5281 ggccgcgtag ccgtcgaggg tggcgtaccg ggcggggtcg tacgcggcgt ggtccgagcc
     5341 gccgtggccc acgtggtcca tcagcaccac gcggtgcgtg tcggagaact ccggcgtgac
     5401 gtaccgccac atctgctggt cgcagccgaa gccgtgcacg aacagcatcg gtcggccgtc
     5461 ctcgcgcccg gacaccgtga cgttgttgcg cccgaaagct cccatgggcg cggaagctac
     5521 ccacgaggcc gccatctcaa agaagcgatt tggggggcaa caccgcgggt acgcacgcga
     5581 tcaaacggat gccccgggcg ccggtgtacc ggcgggaagg cggatgcgga tgacacgggt
     5641 ggggctcgtc ctcggcgcgg gcggcacggt gggtcaggcg taccacgccg gcgtgctggc
     5701 cgccctggaa cacgaccttg ggtgggatcc gcgctccgcc gacgtcatcg tgggcacgtc
     5761 ggcgggctcg atcaccggtg cgctgctccg cctgggcgtg ccggccgcgg agctggcggc
     5821 ctgggcggtc caggcgccgc tgcggctgga cggcggcgcg gtggaggact ggctgagccg
     5881 ggaacggccg cagttcccgc cgttcacccc gcgccactgg ctgcgcccgt ggcgcccgcc
     5941 gtcgccggcg ctcgtggcga agatggcccg ccgcccgtgg tcggtgcgcc ccgcggtggc
     6001 cgcgctgacg tgcgcgccgc gcggccgcgt ggacctgctc gtgcacgccg gcgccctgga
     6061 ggagctcgcg ggcgaatggc cggaagggct gcgcgtgtgc gcggtgcgcc ggcacgacgg
     6121 tgagcgcgtg gcgttcggcg cggagggggc gcccgcggcg gcgctggcca cggcggtggc
     6181 gtcctcgtgc gcgatccccg gctacttcgc ccccgtgcgc atcggcgggc gcgagtacgt
     6241 cgacggcggc gtgcactcgc cgagcaacgc cgacgcgctg gccggcgacg agctggacgt
     6301 ggtgatcgcc gtgtcgccga tgtcgacgtc gacgcgcgtg cgggcgctgg acgcgccggt
     6361 gcgctacgcc gcccaccggc gcctggtgcg cgagctgcgc cgcctggagc ggcgcggcgt
     6421 gacggtggtg cgtttcgagc cgggcgggcg ctccctggcg gccatgggca tcaacatgat
     6481 ggccgacgac cgcgccgacg cggtggtcca ggccgcgttc atcgaggccg gcggcatggc
     6541 ggcgggccgg ctgctggccg accggctggc gccggtgcgc gcacgggccg cggcacagtg
     6601 aggcgttcct caccgtccgg caatggcgga gcaatcggac cgtaacgcgc cggccccagc
     6661 atggccgcat ggcgctcgct ccgctcctgc ccccgctcca gggcttcacg gtcggcatca
     6721 ccgccgcccg tcgctgggag gaccaggcgc agcacctggc cagccggggc gcggagatcc
     6781 tgcatgcccc cgccctgcgc tcggaccccg ccgacccgga gggcgagctc cgccacgtca
     6841 ccgaggcgct ggtgacccgc ccgccggacc tcaccatcct gaccaccggc gcgggggcgt
     6901 cggcatggtt ctccgccgcg gacagcttcg gcgccggcag cgagctgctc gaggcgctgc
     6961 ggcgcggcgc cgtgatccca cgcgggccca agaccgtggc cgcctgcatc caggccgggc
     7021 tcgacgtgcc gcgccggggg ggtcatgagc gagtgcgcga ggtgctggag gcggcgcgtg
     7081 ccggcggcgg tctcgaaggc cgccgtgtcg ccgtgcaact cgacggtgcg gcgacgtcgt
     7141 ggttggtcga tgagctgcgc cgggagggcg cgcaggtcgt ctcggtccgc gtgtaccact
     7201 ggcagccgtc gcacgacgac gagccggtgc gcaagctgat cgacggcgtc tgccgcctgc
     7261 gggtgcacgc ggtcacgttc acgagcacgc cggcggtgcg caacgtgttc tccgtcgccc
     7321 gccaggccgg acgcgacgcg gaactgcgcg agcggttcgc ttccggcacc gccgccgtgt
     7381 cgatcggccc ggtgaccagc gaggcgctgc gccgggaagg cgtcgccgac ccggtcgagc
     7441 cggcgcgcgc gcggctgggc gcgatggtcg ccgcgctggt ccaccacctc gagggccgcc
     7501 ggcgcatcct ggtgctggcc ggatgcgagg tgctgatcca gggggcggcg gtgctcgtcg
     7561 acggcgcgcc gcgggcgctc agcgagcagg atcggcatct gctcgccgtc ctggccgagg
     7621 cgaacggccg ggtggtgccc aagcaggcgc tgctcgaccg cggcctgggc gccggggcgg
     7681 tcagcgagca cgcggtcgag gtcgcggtcg cgaggctgcg gcgacggctg ggccccgcgg
     7741 gcgccggcgt gcagacggtg ttccgccgcg gctaccggct ggcgctggac ggcgccggct
     7801 cgggctcggc cacctcgatc acgccaccgg tcaggcgcac gtcgtagacc gggatgcgta
     7861 ccgcgggatc gtccaggcac gtgccggtgc gcaggtcgaa gcgctccttg tacaccggcg
     7921 aggcgaccac gggcacgccg gcgacgtcgc cgaggatgcc ccgcgacagc acggcggcgc
     7981 cgctgaacgg gtcgacgttg cccacggcgt gcacgtcgcc gtcgcgcagg cggaacacgg
     8041 ccacctgccg gccgcccacg cgggcgcaca ccccgctgtc gggcacgatg tcgtccaggg
     8101 cgcagagcgg gatccagact ccgctcacgc ccggcccccc gtgaccgcgc cggccagccc
     8161 ggtgaccgcg tcggcttggg gcagcgccac gccggccatg tcgaccgcct cccgctcctc
     8221 gggccgggcc ggacggcgct ggccgcgctc gcgcacgtac gccagcgtgg ggtccggcgt
     8281 ctcgtcgttg acgaagtggc ggaaggcccg cagccgcacc ggatcctcca gcgtcgcctt
     8341 ccattcgcac cggtaggtgg cgacgtggcg cgccatgtcc gcctccagtt gctcgcagat
     8401 gccgaggccg tcgtcgatca ccacgcgccg cagatagtcc acgccgccct ccaggcggtt
     8461 gagccaggtg gccgtgcgct ccagccggtc cgcggagcgc acgtagaaca tgaggaagcg
     8521 gtccacgtag cgaaccagcg tctcggtgtc caggtcctcg gccagcagca ccgcatgctg
     8581 gggccgcatg ccgccgttgc cgcacacgta gaggttccag cccttctcgg tggcgatcac
     8641 ccccacgtcc ttgccctggg cctcggcgca ctcccgcgcg cagccggaca cggcgcactt
     8701 gatcttgtgc ggggcgcgca ggccccggta gcgcagctcc aggtcgatgg ccaggctggt
     8761 ggagtcctgg accccgtagc ggcaccagct ctcgccgacg caggacttca cggtgcgcag
     8821 cgccttgccg taggcgtgcc cggactcgaa gcccgcggcc accagccggg cccagatcgc
     8881 gggcagttcg tccaggcggg cgccgaacag gtcgacgcgc tgcccgccgg tgatcttggt
     8941 gtacaggtcg tggtcgcgcg cgacctcgcc cagcacgatg agctgcgccg gcgtcagctc
     9001 gccgcccggc acccgcggca ccaccgagta cgtgccgtcc ttctggaggt tggccaggaa
     9061 gcggtcgttg gtgtcctgga ggggcaggtg ctcgtcggcg agcacgtact cgttccagaa
     9121 ggacgcgagg atggaggcga ccgcgggctt gcacacgtcg cagccgcgtc cggtccccca
     9181 gccgtccagg agctcggcga aggtgcggac ctcgcgcacg cgcacgatgt ccgccagctc
     9241 ctgccgggtg tgggggaagt gctcgcacac gctgcggtcg accgcgacgc ccgcccgctc
     9301 cagctcgtgc tccaggatgc ccttgaccag cggcacgcag ctcccgcagc cggtgcccgc
     9361 ggcggtgcag gccttgacct cgcccaccgt ggtcagcccc tcaccggcga tggcggcgca
     9421 gatggccgcc ttggagacgt tgttgcaatt gcagacggtc gactccatcg ggatcgccgc
     9481 gggacccagc gccgcgccgc cgccggcgcg cgcgggcagg accaggtccg cgggatcgcc
     9541 cggcagcggc agcgccgaca ggcagcgctg gtgcagggcg gcgtagtcgg cggcgtcgcc
     9601 gaccaggatg ccgccgagca cgcgggtgcc ctcgccgtcc accaccagcc gccggtacac
     9661 gcgcgccatc gggtcgctcc acaccaggct gcgcgcgttc tcggccgtcc cgtgcgcgtc
     9721 gccgaagctc gcgacgtcga cgccgagcag cttgagcttg gtggacatgt ccgcgggcgc
     9781 gaagaccgcg tcgccgccca ggagccggcc ggccaccacc cgcgccatcg cgtagccggg
     9841 ggcgaccagg ccgtagacgc ggccgccgaa gctggcgcac tcgccgatcg cgaagatcgc
     9901 cgggtcggaa gtccggcacg cctcgtcgac caccacgccg ccgcgctccg ccacgtcgag
     9961 gccgcacgcg cgcgcgagct cgtcgcgtgg gcggatgccg gcggagatga ccaccacgtc
    10021 cgcgtccagc gcgtcgccgt cgctgaactc caggcgggcg ccgccgggac cgtgcacgat
    10081 ccgctccgtc accgcaccgg tgcgcacgcc gatgcccagc gcctcgatct cctggcgcag
    10141 gatggccccg ccgccgtcgt cgacctggac cggcatcagg cgcggcgcca tctccaccac
    10201 gtgcgtctca aggcccaggt ggcgcagggc gttcgcggcc tccagtccca gcaggccccc
    10261 gcccagcacc acgcccacgc gcgcgccggc cgcggccgcg cggatggcgt ccaggtcgtc
    10321 cagcgtgcgg tacacgaagc agccgggccc gcgcgcgccc tcgatcggag gcacgaacgg
    10381 cgcggagccc gtggccagca ccagcgcgtc gtagcgctcc cgcgacccgg aggcggtggc
    10441 caccgtgcgc gccgcgcggt ccacggccgt cacgggatcg cccaggacca gctccactcg
    10501 ctccgccacc tccggcgaca gcaggctcag gtcctcggcc gagcggccgt cgaagtaggt
    10561 gctcaggttc acgcggtcgt aggcggcgcg gggctcctcg ccgaagacga cgacgcgcca
    10621 ggcgtcggcg cctccggcct ccacgaggca gtccagcagc ttctggccga ccatgccgtt
    10681 gccggcgacg acgagggtgg gattcatgga gggcacggta ggcgggggct gtttcgccac
    10741 ggttgggccc gtgcaacggc cgcgtgaagt ccggcgcaca ggcggcaagc gccgcggtgc
    10801 gggggaattg cggcgcgcgt aacgcggcgg tatcggccgg gaaaccgcgg ggccgcagca
    10861 tcccgcgcca tgaccacctc acacgatgcc gccacccgcc tgctggccct gcgctcgttc
    10921 accggcaggt accgggtcct gcacctgacc tggctcgcgt tcttcctgac gttcgtcgtc
    10981 tggttcaact tcgcgccgtt cgcctccacc gtgagcgcgg ccttcggcct gtccggcgag
    11041 caggtggcgg tgctcgccct gtgcaacgtc gccctgaccg tgcccgcgcg cgtgctggtc
    11101 ggcatggcgc tggaccgctg ggggccccgg cgggtgtacg cggccatcct gatgtacgcc
    11161 gccgtgccgt gcctggcgtt cgccgccgcg acgagcttcg gcatgctggt cgcgagccgt
    11221 ctcgccctca gcgtggtggg cgcgggcttc gtggtgggca tccgcatggt cgccgagtgg
    11281 ttcccgccgc gggaggtcgg catggccgag ggcgtctacg gcgggtgggg caacttcggc
    11341 tcggccgccg cggcgctcgc gctgcccacg ctggcggccg tcctgggcgg cgccgacggc
    11401 tggcgctggg cgatcggcag caccgggatc gtgtcggccg cctacggcgc ctggtacctg
    11461 cgtgccgtca gggacacgcc cgacggcctc ccgctggtgc ggccgcgccg ccagggagcg
    11521 ctggaggtca cccaccctgg cgcggtcgcc ggtctgctcg ccctgaacct gccgctgctc
    11581 ggcgcgctgg gcctggtggc gtggcggctg gcccgcgtgg gcttcctctc gggagcgggg
    11641 ctgacggccg tggtggtgac gctgggcgcg ctggccgtcc tccaggcccg caccgtgtgg
    11701 cgggtcaacg cgccggcgcg ccaggacgcc tacgaccccg gcgaccgcta cccgttccgg
    11761 tccgtggtgg tgctctgcct ggcttacgcg gtgaccttcg gctcggagct cgcggtcgtg
    11821 tccatgctgc ccacgttctt cgcgggcacg ttcgggctgt ccacggtcgc ggcgggcgcg
    11881 accgcgagcg cgttcgcctt catgaacctg ggagccaggc cggcgggtgg gctgctgtcg
    11941 gacctgctcg gcagccgccg ccgcacgctc accgcgatgc tgctgggcct gtcgggcggc
    12001 tacgcgctga tggccgcggt cgggtcgggg tggccggtgc cgctcgcgat ggtcgcggcg
    12061 atgacgtgct cgctgttcgt gcaggcgggc gagggcgcca cctacgccat cgtcccgctg
    12121 gtgaagcggc gcgtctcggg gcagatcgcc ggcctggtcg gcgcctacgg gaacgtgggc
    12181 gcgctggcct acctcacgct gctgctgttc gccgggccca cggtgttctt cctggcgatc
    12241 agcgcgtccg ccatcgccgc tgcgatcgcg tgccggtggc tggtcgagcc ccagggcagc
    12301 ttcgcgcacg tgcacgagcc ttcggacgcc gtcacggccg cgcccgcgct ggaggagcgc
    12361 atcgcgtgac ggacctgctg acgcccctgg accgctacct ggcggagcag cgcggcacca
    12421 cggcggtggg acgcttcgcc cggtggcacg agcgggacgg cggagggcgg ctggcggcgc
    12481 tcacccacta ccgggagctg gtgccgctgg agcggccggg cccgggtgag cagtacgcct
    12541 tccaggtgga cctggactcc tgcaccggtt gcaaggcgtg cgtgagcgcc tgcaaccaca
    12601 tgaacggcct cgacgagggc gagtcgtggc gctccgtggg cctgctggtc ggcagccgcg
    12661 gcgaccagcc gttccagcag tccgtcaccg cgggatgcca ccactgcgcc gagcccgcgt
    12721 gcctgtccgg ctgccccgtc gacgcctatg agaaggatgc cgagaccggc atcgtccacc
    12781 acctggacga ccagtgcatc ggctgccgct actgcattct catgtgcccg tacgaggtac
    12841 cgacgttctc cgaccggctg ggcatcgtgc gcaagtgcga catgtgcagc gggcgcctcg
    12901 cggccggcga ggcgcccgcc tgcgcgcagg cctgccccaa tggggcgatc acgatcaccg
    12961 tcgtggcccg cgcggaatcc gccgcgcggg ccaccggggg cgaggcgacg gtgcccggcg
    13021 cgcccgaccc gcgcatcacg ctgcccacga cggtgtaccg caccacgcgg ccggccctgc
    13081 tcgcctcgga ggcggcggac cggtccagcg ccgagccggc gcacgaccat cccccgctgg
    13141 tggtcatgct ggtgctgacc cagctgtccg tgggcgcctt cgccaccgac tacctgctga
    13201 cggtgctcga ccgccgcgtg gtggccgggc ccttcccggc cgcgttcgcc ctggccgccg
    13261 ggctgctggc gctgggcgcc agcctgctgc acctgggcag gccgcggtac gcctaccgcg
    13321 ccgtggtcgg gctgcgccac tcctggctga gccgtgagat cgtcgcgttc ggcgggttcg
    13381 ccggcgtcgc ggccgcctac gccgcggcca ggtggctgac gccggggacg atgaccccgc
    13441 cgatgctggc actcggcgcc ctggtggtgc tgacgggcgc ggcgggtgtg ggctgttcgg
    13501 tcatgatcta cgcggtcacg cggcgcacgt ggtggcgcgc cgccatgacc gcgccccgct
    13561 tcgccctgtc gggcgcggtc tccggcgcct cggtcgtcat ggccgccggc gtggtcaccg
    13621 cggtgctggg cggcgcgacc gccctgtccg cccccaccgg cccgctggcg ctgaccgtga
    13681 tgggcgccac cgccgccaag ctcgccctgg aggcggaggt gctgcgcaac cggcgcgccg
    13741 gcagcccggc ggacctcgcg ggcaccgccc gcctgctggc cggcccgctg cgcggcctcg
    13801 tccagcgccg cgtcgcggcc ggcatggccg gcgggatcgt cgtccccggc atcctcgcgc
    13861 tgctggcgac cgaaccggaa gcccccgtgg gggtggccgc cgggttcgcg gtgacgggtg
    13921 ccctgctggt gctcgcgggg gagttgtggg ag
//
LOCUS       DATMKC010000250         5817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_72012, whole genome shotgun sequence.
ACCESSION   DATMKC010000250 DATMKC010000000
VERSION     DATMKC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72012"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VNU01_07330"
     CDS             <1..307
                     /locus_tag="VNU01_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32467.1"
                     /translation="GPAAQARDSGGALRAAEQDTGPIALIAPPEPVAHLPVGDLPPWP
                     VDTPAGALAGRPADAPADTRFEAPPGALAPRMRRGVGLLLVGTALGMLAGAVSLVLR"
     gene            complement(309..1058)
                     /locus_tag="VNU01_07335"
     CDS             complement(309..1058)
                     /locus_tag="VNU01_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWH32468.1"
                     /translation="MATDQAALGSRTLARGLRVLKCLGIAPEGATVAELAAATGLDRA
                     VLYRLLDTLADEGFVVRDGDTRRYHLGVALVELGARAGRGLEVRRIALPAMRALMDQV
                     REAVCLTVRDRSDVVVVDRVEPPGLFVRVGYHVGFRHPITVGAHGRALLAFMDGDDRA
                     VHLERQPQVAAELDTCRERGYAVSLDELERGASGVAAPILDRLGRPIAAVGVVAPSPR
                     MSDPGALGPRVRALALEISRRLGYAPSAEQS"
     gene            1285..1857
                     /locus_tag="VNU01_07340"
     CDS             1285..1857
                     /locus_tag="VNU01_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWH32469.1"
                     /translation="MSDEIKNPLESMTEASVFKKLYDNRILYLRGPIEDTVADTLVAQ
                     MMSLDAESDKDITLYINSPGGLVSGMFAIYDVMELMNSKVNTVCVGIAASAAAFLLST
                     ATGSRAATPNARIMFHQPLGGARGQASDIQIQAQQIAFLRERLYEILAERTGQTVERI
                     RTDADRDFWLSAEEAVAYGAIDEVRRRGGV"
     gene            complement(1815..4061)
                     /locus_tag="VNU01_07345"
     CDS             complement(1815..4061)
                     /locus_tag="VNU01_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWH32470.1"
                     /translation="MSTDGPRLPLRLVGRPDVHRAASAYLDAGLDMAYQPIVHLGTGS
                     VLAYEALARPRHSEAGGPQEFFAALEEAGMRLDGERAAFDAAMQGLLTGEDAFPRVKL
                     FVNASPTTLMDAAFDVQEFVALAERAGLAPSDLVVEVTESEAIDDLESLARRAERLRH
                     LGVALAVDDAGAGHASFRVITRLRPAYIKIDRDLVREVDQDGARHAFIEAMVRFSREI
                     GSRLIAEGIETEAELASLAGLGVEAGQGWFLARPAIGVWEAPTPDSRRVIALAAQRPR
                     LGGAQVTVGHLATPALVLEPGATLAQAHARFAAEPTAGLLVATDHNRVVAQLSRRAVD
                     RVAAAGSWEHVAHRPATEFADHQARTVQSELDVVEVGTILAARERDDLIEDVVVTDAL
                     GRLVGVVSLRDVLRSLADVRKRAERDLNPLSGLSGPGWLEAELERRLLAEDALVALWV
                     DLDGFRRINDSGGFALGDDVLRVMGQALRGVCAGVPGAEPFHAGADDFVVLVPPSRYE
                     ETLSELVRSFEHEVLPFVRTELRLRAAGGVVAVVALSMAAVDLVGRPPPGHRHMQWVQ
                     DQLAGLIPLSKGQPGHCCVRRGGDQLHVTTWSPDPCDLREVDVGEADPGVVLGALALA
                     ERSWAGWLSENGGNRFPGPRAELQSLHERYLRPVRRLAERALATGTTVELRVTGHEDE
                     LLELLDRLSLVVRRAHLAQRAPTPPELALLDRLVRFRARRLQRTDRLDRGALAQTPPR
                     RRTSSIAP"
     gene            complement(4154..4984)
                     /gene="mutM"
                     /locus_tag="VNU01_07350"
     CDS             complement(4154..4984)
                     /gene="mutM"
                     /locus_tag="VNU01_07350"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWH32471.1"
                     /translation="MPELPEVESVRRQLAPRLEGRRIEAVHAAPQARFDDVDEAAGRR
                     VHVLRRRGKYLIAELEGGLELVMHLGMTGSFRFRGDPWPAPGGESRPWGGDAYVRATF
                     TLDDGVLDFRDVRRFGRLSVVPAGEYAGIETLAKLGPEPLSEAFDPGGFARALAGTST
                     MVKPYLLSQRPVAGVGNIYADEALWRARIHPCARAVGPRRARALWQAIREVLAESVER
                     EGTTFRDYLMVNGESGRNAAFLVAYGQAGRPCPRCATPLRKTVVAGRGTTYCPRCQRH
                     "
     gene            5232..5633
                     /locus_tag="VNU01_07355"
     CDS             5232..5633
                     /locus_tag="VNU01_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32472.1"
                     /translation="MIQPIATISASGAVRDQQPLDREAAALFLRAQASWLRRGLRLAE
                     DPVEVGIVLGTHDSSHESWVELIAAVERSAGAPAAGVVSALYGFHGRVVGSLERDMDL
                     RLDAAVQAQVRRMLADRLTEMTEQAVALLAR"
BASE COUNT          775 a         2246 c         2098 g          698 t
ORIGIN      
        1 gggccccgcc gcgcaggcac gggactccgg cggcgcgttg cgggcggccg agcaggacac
       61 tggccccatc gcgctcatcg ccccgccgga gcccgttgcg cacctgccgg tgggcgactt
      121 gccgccctgg ccggtggaca cgcccgcggg cgccctggcc ggccggccgg cggacgcgcc
      181 cgcggacacg cgcttcgagg cgccgccggg cgcgctcgcg ccgcgcatgc gccgcggagt
      241 gggcctgctc ctggtcggca ccgcgctggg catgctggcc ggcgccgtct cgctggtgct
      301 gcgctgagtc aggactgctc ggccgagggc gcgtagccca gccggcggga gatctccagc
      361 gccagggcgc gcacccgcgg tcccagggcc cccggatcgc tcatgcgcgg gctgggcgcc
      421 acgacgccga ccgcggcgat cggccggccc aggcggtcca ggatcggcgc ggccacgccg
      481 gaggccccgc gctccagctc gtccagcgac accgcgtagc cccgctcccg gcaggtgtcg
      541 agttcggcgg ccacctgcgg ctggcgctcc aggtgcacgg cgcggtcgtc accgtccatg
      601 aacgccagca gcgcccgccc gtgcgccccg acggtgatcg ggtggcggaa cccgacgtgg
      661 tagcccaccc gcacgaacag gccgggcggc tccacgcggt ccaccaccac gacgtcgctg
      721 cgatcccgca cggtcaggca caccgcctcg cgcacctggt ccatcagcgc gcgcatggcc
      781 ggcagggcga tgcggcgcac ctccaggccc cgcccggccc gcgcgcccag ttccaccagc
      841 gccacgccca ggtggtagcg gcgggtgtcg ccgtcgcgca ccacgaagcc ctcgtcggcg
      901 agcgtgtcga gcagccggta cagcaccgcg cggtccaggc cggtcgccgc cgccagctcg
      961 gccacggtcg cgccctccgg tgcgatgccc aggcacttga gcacgcgcag gcctcgcgcg
     1021 agtgtgcgcg agccgagcgc tgcctggtcc gtcgccacgt gccgtttccc ctcgagcgcg
     1081 ccggcacgcc aagaccgggg cgctccccac tcgcaccccg gtgagggcgc ggtctcccgc
     1141 ggcgagtgtg ccagcgcgtc gtgaagcgtc aatgggtaac cgtcggtacg ttcccgccgc
     1201 gctgaagggg aagggacccg ctgctaggct tcgcctcccc gcggcggctg ccggccgcgg
     1261 tggcctgacg aggagcggaa cacggtgagc gacgagatca agaacccgct ggagtcgatg
     1321 accgaggcct cggtcttcaa gaagctctac gacaaccgca tcctctacct gcgcggtccc
     1381 atcgaggaca cggtggccga cacactggtc gcgcagatga tgtcgctgga cgcggagagc
     1441 gacaaggaca tcaccctcta catcaactcc cccggcgggc tggtgagcgg catgttcgcc
     1501 atctacgacg tcatggagct gatgaactcc aaggtcaaca cggtgtgcgt cggcatcgcc
     1561 gcgtccgcgg cggcgttcct gctgtcgacg gccaccggca gccgggccgc cacgcccaac
     1621 gcccgcatca tgttccacca gccgctcggc ggcgcgcgcg gtcaggccag cgacatccag
     1681 atccaggcgc agcagatcgc gttcctgcgc gagcggctct acgagatcct ggccgagcgc
     1741 acgggccaga ccgtggagcg gatccgcacc gacgccgacc gcgacttctg gctgtcggcc
     1801 gaggaggccg tcgcctacgg ggcgatcgac gaggtccgcc gccgcggcgg cgtctgagcc
     1861 agcgcgccgc ggtccagccg gtcggtgcgc tgcaggcggc gcgcccggaa gcgcaccagc
     1921 cggtccagca gggccagctc cggcggcgtc ggcgcgcgct gcgccaggtg cgccctgcgc
     1981 accaccagcg acaggcggtc caacagctcc agcagttcgt cctcgtggcc ggtgacgcgc
     2041 agctccacgg tcgtgccggt cgccagggcg cgctcggcga ggcgccgcac cggccgcagg
     2101 tagcgctcgt gcagcgactg cagctccgcg cgcgggccgg ggaagcgatt cccgccgttc
     2161 tcgctcagcc agccggccca cgagcgctcg gccagggcca gcgcccccag gaccacgccg
     2221 gggtcggcct cgcccacgtc gacctcgcgc aggtcgcacg ggtcgggact ccacgtcgtc
     2281 acgtgcagct ggtcgccgcc gcggcggacg cagcagtggc ccggctggcc cttgctgagc
     2341 gggatcagcc cggcgagctg gtcctgcacc cactgcatgt ggcggtgccc cggcggcggg
     2401 cggccgacca ggtcgaccgc cgccatcgac agcgcaacga cggccaccac cccgccggcg
     2461 gcccgcagcc gcagctccgt gcgcacgaac ggcagcacct cgtgctcgaa gctgcgcacc
     2521 agctcgctca aggtctcctc gtagcgggac ggcggcacca gcaccacgaa gtcgtccgcg
     2581 ccggcgtgga acggctccgc gcccggcacg cccgcgcaca cgccgcgcag cgcctgcccc
     2641 atcacgcgca ggacgtcgtc gcccagggcg aagccgccgc tgtcgttgat gcggcggaac
     2701 ccgtccaggt ccacccacag cgccaccagg gcgtcctcgg ccagcaggcg acgctccagc
     2761 tccgcctcca gccagccggg gccggacagg cccgacagcg ggttgaggtc gcgctcggcg
     2821 cgcttgcgca cgtcggccag ggagcgcagc acgtcgcgca ggctcaccac gccgaccagc
     2881 cgccccagcg cgtcggtcac caccacgtcc tcgatgaggt cgtcgcgctc ccgggcggcc
     2941 aggatcgtgc cgacctccac cacgtcgagc tcggattgca ccgtgcgggc ctgatggtcg
     3001 gcgaactccg tggcggggcg gtgggcgacg tgctcccagg agccggcggc ggccacgcgg
     3061 tcgaccgcgc ggcgtgacag ctgcgccacc acgcggttgt ggtcggtggc cacgagcagc
     3121 cccgccgtgg gctcggcggc gaagcgcgcg tgggcctgtg ccagcgtggc ccccggttcc
     3181 agcaccagtg cgggcgtggc caggtggccc acggtcacct gcgcgccgcc cagcctgggc
     3241 cgctgcgcgg ccagcgcgat gacgcggcgc gagtccggcg tgggcgcctc ccacacgccg
     3301 atggcgggcc gcgccaggaa ccagccctgc ccggcctcca cgccgagccc cgccaggctg
     3361 gccagctccg cttccgtctc gatgccctcg gcgatcaggc gcgagccgat ctcccgcgag
     3421 aagcgcacca tcgcctcgat gaaggcgtgg cgcgcgccgt cctggtcgac ctcgcgcacg
     3481 aggtcgcggt cgatcttgat gtaggcggga cgcaggcgcg tgatcacccg gaacgaggcg
     3541 tggccggccc cggcgtcgtc gaccgccagc gccacgccca ggtggcgcag gcgctcggcg
     3601 cggcgcgcca gcgactccag gtcgtcgatc gcctcggact cggtgacctc gaccaccagg
     3661 tcggagggcg ccaggccggc ccgctccgcc agcgccacga actcctgcac gtcgaaggcg
     3721 gcgtccatga gcgtggtggg gctggcgttg acgaacagct tcacgcgcgg gaacgcgtcc
     3781 tcgcccgtga gcagcccctg catggcggcg tcgaaggcgg cgcgctcgcc gtccaggcgc
     3841 atgccggctt cctcgagggc ggcgaagaac tcctgcggcc cgccggcctc gctgtggcgc
     3901 gggcgcgcga gcgcctcgta cgccagcacc gacccggttc ccaggtgcac gatgggctgg
     3961 taggccatgt ccaggcccgc gtccaggtac gccgaagcgg cacgatggac atccggccgc
     4021 ccgacgaggc gcaggggcaa gcgcggccca tcagtggaca tacgaccagt ctcggcgtcc
     4081 gtacggggaa tctgtagggc taactcggat ccagtccatt ggcactagga cgccggttcc
     4141 cggagcgtcg cggtcagtgc cgctggcagc gcgggcagta cgtcgtgccg cgccccgcca
     4201 ccaccgtctt gcgcagcggc gtggcgcagc gcgggcacgg gcggccggcc tggccgtagg
     4261 caaccaggaa ggcggcgttg cgccccgact cgccgttcac catgaggtag tcgcggaacg
     4321 tcgtgccctc ccgctccacc gactcggcca gcacctcgcg gatcgcctgc cacagcgcgc
     4381 gggccctgcg tggccccacg gcgcgggcgc acgggtggat gcgcgcccgc cacagcgcct
     4441 cgtcggcgta gatgttgccc acgcccgcca ccggccgctg gctcagcagg tacggcttca
     4501 ccatggtgct ggtgcccgcc agcgccctgg cgaagccgcc ggggtcgaac gcctccgaca
     4561 acggctccgg ccccagcttg gccaaggtct cgatgccggc gtactccccc gccggaacca
     4621 ccgacaggcg gccgaagcgg cgcacgtcgc ggaagtccag cacgccgtcg tccagcgtga
     4681 acgtggcgcg cacgtaggcg tcgccgcccc acgggcggct ttcgccgccc ggcgccggcc
     4741 acgggtcgcc gcggaagcgg aacgagccgg tcatgcccag gtgcatcacc agctccaggc
     4801 cgccctccag ctccgcgatc aggtacttgc cgcggcggcg gaggacgtgg acgcggcgcc
     4861 cggcggcctc gtccacgtcg tcgaagcgcg cctgcggcgc cgcgtgcacc gcctcgatgc
     4921 ggcggccctc caggcgcggg gccagctggc ggcgcacgct ctcgacctcg gggagctccg
     4981 gcatgcggcc atgatgccgc gccaggccgc ccgcgcgcgg agccgcgcaa gcggccgtgc
     5041 cgcctccacc gatcagaacg agtcccctgc gatgctgcac cggtactgcg cggaccatgc
     5101 cggcctagtc cttcaggact acattcggct atttccatgc acagagagtg aagcgggttt
     5161 acgggtttgc cgaaccgtac ggcaacggct cccgggcagt cacgggagcg ccggatcgga
     5221 gggcgcacag cgtgatccaa cctatcgcga cgatatccgc cagcggggcg gtgcgggacc
     5281 agcagccgct ggaccgcgag gcggcggccc tgttcctgcg agcgcaggcg tcgtggctgc
     5341 gccgcggcct gcggctggcg gaggaccccg tggaggtggg catcgtcctg ggcacccacg
     5401 acagctccca cgagtcctgg gtcgagctca tcgccgcggt ggagcgcagc gcgggcgcgc
     5461 ccgccgccgg cgtggtctcc gccctctacg gcttccacgg ccgggtggtg ggctcgctgg
     5521 agcgcgacat ggacctgcgc ctggacgcgg ccgtgcaggc gcaggtgcgg cgcatgctcg
     5581 ccgaccggct caccgagatg accgagcagg cggtggcgct gctcgcgcgc tagcgaaggc
     5641 tagcgaggag gcatgcgcag agcgccgtcg aggcggatca cctcgccgtt gagcagcggg
     5701 ttctccacga tctggcgcac catgagcccg tactcggccg ggtcgcccag cctgctggga
     5761 tgcgggatcg ccctgcccag cgcctggcgc gtctcctccg gcagtgccgc cagcatc
//
LOCUS       DATMKC010000251         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_148712, whole genome shotgun sequence.
ACCESSION   DATMKC010000251 DATMKC010000000
VERSION     DATMKC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148712"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VNU01_07360"
     CDS             complement(<1..1343)
                     /locus_tag="VNU01_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32473.1"
                     /translation="MRATETEPIAPGAAAEGALRTLRRGLRLSPELRAGMGLTLLVAV
                     VATAGRVIAPVAVQLTIDQGLLAEGGPRFDRVLTLVALGVAAVAVTGACGGLMQYRLS
                     RASETALSALRVRAFRHVHDLSALHQSGEHRGALVARVTADVNTISHFMEWGGMVLVV
                     AVLQLGLATAVMLLYSPLLTAVVLGLFVPLVLALRWFQRILRRAYDEVRVRLSFLLAA
                     LAESVVGAPVIRAYGATARTEERIGSAVTRHFRAAFRAGRLGALMFASGELFAAAAMA
                     GVVVVGVHAGVAGELTPGRLVAFLFLVTLFVGPVQIATEVLDMAQSAIAGWRRVLDLL
                     DTAPDVTDPAGSPGGGRPIPPGPVEVRFAHVWFAYPAAPDTPVLRDVDLLIAPGTRVA
                     VVGETGSGKTTFAKLLTRLMDPAGGRVLLNGVPADEVPFADLRHRVVMVPQDGFL"
     gene            complement(1340..>2113)
                     /locus_tag="VNU01_07365"
     CDS             complement(1340..>2113)
                     /locus_tag="VNU01_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWH32474.1"
                     /translation="SFSYDPSGAGAWAETAGGAGHGAPADGGVPALRDIGFHVRPGSV
                     VALVGPTGSGKSTIASLLVRLADPQSGEVRLDGRDVRALTRETLARDAALVFQHSFLF
                     DDTVRGNITLGEAFSDQEVREAAALAQAHHFIERLPNGYDTMVGERGATLSGGQRQRV
                     ALARALVRRPRLLVLDDATSSVDATVESAILAGLRAAPLPSTIVVVAYRRATIALADE
                     IVYVEDGLVRARGTHAALLSSVPGYADLVTAYDEPAGAR"
BASE COUNT          305 a          811 c          783 g          214 t
ORIGIN      
        1 aacaggaaac cgtcctgggg gaccatgacc acgcggtggc gcaggtcggc gaagggcacc
       61 tcgtcggcgg gcacgccgtt gagcaggacc cggccgccgg ccgggtccat caggcgcgtc
      121 agcagcttgg cgaacgtagt cttgccggag ccggtctcgc cgaccacggc cacgcgcgtg
      181 ccgggtgcga tcagcaggtc gacgtcgcgc agcacgggcg tgtcgggggc cgccgggtag
      241 gcgaaccaca cgtgcgcgaa gcgcacctcg accggccccg gcggaatcgg ccgcccgccg
      301 ccgggggatc ccgccgggtc ggtcacgtcc ggcgccgtgt ccagcaggtc caggacgcgc
      361 cgccagccgg cgatcgcgga ctgcgccatg tcgagcacct ccgtcgcgat ctgcaccggc
      421 cccacgaaca aggtcaccag gaacaggaac gccaccaggc ggccgggcgt cagctcgccc
      481 gccacgcccg cgtgcacgcc gaccaccacc acgccggcca tggcggcggc ggcgaacagc
      541 tcgccgctgg cgaacatcag ggcgcccagc ctgcccgccc ggaaggcggc gcggaagtgc
      601 cgcgtgacgg cgctgccgat gcgctcctcc gtgcgggcgg tggcgccgta cgcgcggatc
      661 accggcgcgc ccacgacgga ttcggccagg gcggccagca ggaagctcag ccgcacccgc
      721 acctcgtcgt aggcgcggcg caggatgcgc tggaaccagc gcagcgccag caccagcggc
      781 acgaacaggc ccaggaccac ggccgtcagc agcggggagt agagcagcat cacggcggtc
      841 gcgagcccca gttgcagcac ggcgaccacc agcaccatgc cgccccactc catgaagtgg
      901 ctgatggtgt tcacgtccgc ggtcacccgg gcgaccagag cgccgcgatg ctcgccgctc
      961 tggtgcagcg cagacaggtc gtggacgtgg cggaacgcgc gcacgcgcag ggcggacagc
     1021 gccgtctccg aggcgcgcga caggcggtac tgcatgagtc cgccgcacgc gccggtcacg
     1081 gcgacggccg ccacgccgag cgccaccagc gtgagcaccc ggtcgaagcg cggtccgccc
     1141 tcggccagca gcccctggtc gatggtgagc tggaccgcga cgggggcgat caccctgccc
     1201 gccgtggcga cgaccgccac cagcagcgtc agccccatgc cggcgcgcag ctccggcgac
     1261 aggcgcaggc cgcggcgcag ggtccgcagc gcaccctcgg ccgccgcgcc gggcgcgatc
     1321 ggctcggtct cggtggccct catcgcgcgc ccgcgggctc gtcgtaggcc gtgaccaggt
     1381 ccgcgtagcc ggggacggac gacagcagcg cggcgtgcgt cccgcgcgcg cgcacgaggc
     1441 cgtcctccac gtagacgatc tcgtcggcca gcgcgatggt cgcccgccgg taggccacca
     1501 cgacgatcgt cgagggcagc ggcgcggcgc gcaggccggc gaggatcgcc gactccaccg
     1561 tggcgtcgac cgacgaggtc gcgtcgtcaa gcacgagcag ccgcggccgg cgcaccagcg
     1621 ccctggccag cgccacgcgc tggcgctgcc cgcccgacag cgtggcgccg cgctcgccca
     1681 ccatcgtgtc gtagccgttc ggcagtcgct cgatgaagtg gtgcgcctgg gcgagtgcgg
     1741 ccgcctcccg cacctcctgg tcgctgaacg cctcgcccag ggtgatgttg ccgcgcaccg
     1801 tgtcgtcgaa caggaacgag tgctggaaca ccagcgcggc gtcgcgcgcc agcgtctcgc
     1861 gggtgagcgc gcgcacgtcg cggccgtcca ggcggacctc gccggactgc gggtcggcca
     1921 ggcgcaccag cagcgaggcg atcgtggact tgcccgagcc ggtggggccg accagcgcca
     1981 ccacgctgcc ggggcgcaca tggaagccga tgtcgcgcag cgccgggacg ccgccgtcgg
     2041 ccggtgcgcc gtggcccgcg cccccggcgg tctccgccca cgcgcccgcg ccggaggggt
     2101 cgtagctgaa gga
//
LOCUS       DATMKC010000252         4980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_50215, whole genome shotgun sequence.
ACCESSION   DATMKC010000252 DATMKC010000000
VERSION     DATMKC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50215"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /gene="uvrB"
                     /locus_tag="VNU01_07370"
     CDS             complement(<1..1929)
                     /gene="uvrB"
                     /locus_tag="VNU01_07370"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWH32475.1"
                     /translation="MPAFEIASDFQPAGDQPRAIEGVVKALAAGESDVVLLGVTGSGK
                     TFTASKIVESVQKPTLVIAPNKTLAAQLANEFREFFPRNAVEYFVSYYDYYQPEAYVP
                     SSDTYIEKDSSVNEEVERLRHRATMALMSRRDVLIVASVSCIYGLGSPEEYSKHVLML
                     NQGEDHGLDLVLRKLVDLQYERNDLNFVRGKFRLRGDTLEVFPADSELAYRVEFFGDT
                     VDRITRMDPLTGEVVEEVAQAGVFPNTHYVADRQRTERAIGTIQHELEERLKELEGQN
                     KLLEAQRLRMRTNYDLEMMREIGFCSGIENYSRHIDGRAPGEAPFTLLDYFPDDFLCV
                     IDESHVTVPQIGGMYEGDRSRKQTLVEHGFRLPSALDNRPLTFEEFRSRVPQTLYVSA
                     TPGPFEKRVSTTVVEQVIRPTGLVDPEVVVRPTKGQIDDLIAEVRARAERDERVLVTT
                     LTKKMSEDLTDYLLESGVRVRYLHSEIDTVQRIDILRQLRMGEFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSGTSLIQTIGRAARNVNGSVIMYADSLTDSMKRAIDETN
                     RRRRLQTEYNAEHGIDPQSIRKRVGDIIQAARAAEAGVRYEPHDPRKAAVPNLRTKRK
                     RAGGDGVPEISDLPQEDLE"
     gene            complement(1973..3718)
                     /locus_tag="VNU01_07375"
     CDS             complement(1973..3718)
                     /locus_tag="VNU01_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWH32476.1"
                     /translation="MADYDVIIIGAGGGGPVAAKELAEQGLRILHLEAGGYARDPEHD
                     WSHSEADMNNVVSGQFRWGPSDRSRSPWVRRMVGSGLVQQVSGVGGTTQHYFANSPRA
                     YPLAVERGAWPHAYADLVPYYERVEGILPVVRDPALPTKDAWAVYGAHKLGLPQINGK
                     DVHRAGWRPQYNAIIPPGHFGAGTGCNQCGHCYEGCMHPHGAALAAKAKRGTNVSYVP
                     LAEEHKGYQLETDAFATKILTGTRDGAPAATGVQWRNTRSGEVQEATAEVVILAAGCV
                     ESPRLWLNSGLPNSTDSVGRNLTLHWFDFVTGYFPFPIHPAVGQNSQSRIEFPGLGCL
                     EVVGLGPGKTAFGTMTFSNSWAMDDNSQGEPWSTRGHVVGGALRERMRQHDRSLTLLV
                     LTDDEISRDNRVTLSQNWAPDEHGAVPQVQYTPTPESDRRRDELCRQAANILRAAGAS
                     DVHRADWPPLYLHMQSSMRMAATPDNSVVDRNQESWEVKRLFVCDASSLPDGLGGPNP
                     TLSAQMFATRTAEYIAQTYFDRDPFVLEGTGRLTPAGAGPELPATGGGPGGVLGGAAL
                     AAGAAATLHQLSRRP"
     gene            complement(3728..4336)
                     /locus_tag="VNU01_07380"
     CDS             complement(3728..4336)
                     /locus_tag="VNU01_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32477.1"
                     /translation="MTTINRRELLSAAVKLAGGAFALSVPEVLYRTPWTELAQAQTLP
                     TVERAFIALIAAVNDAPEGDERTIAVARWVIAEFDKALPPLPDRSVTAAVAAVLDAYA
                     LKGGHGAGFAQADTDGRLATLGDMVKDPSPDIQQIANQILPFAGFAYWGDASLGRPSV
                     PGDTMPQWTEIGWSGPSHGHLDDFQDDWPQDPSFRPRGHAGP"
     gene            complement(4419..>4980)
                     /locus_tag="VNU01_07385"
     CDS             complement(4419..>4980)
                     /locus_tag="VNU01_07385"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32478.1"
                     /translation="GIDAALAAGLGVKLNTVVRRGVNESEILPLAAYGRERGVTVRFI
                     EYMDVGASNGWRHDDVVPAAELLARLAARWPLEPVAPAYPGEVATRWRYADGAGEIGL
                     IASVTQPFCGDCTRARLSAVGEVFTCLFAASGHDLRPLLRGGAADDALREEVARLWGA
                     RDDRYSELRSAGAPQLPRVEMSYIGG"
BASE COUNT          644 a         1801 c         1772 g          763 t
ORIGIN      
        1 ttccaggtcc tcctgcggca ggtcgctgat ctccggcacc ccgtcgccgc ccgcgcgctt
       61 gcgcttggtg cgcaggttcg gcacggcagc cttgcgcgga tcgtggggct cgtagcgcac
      121 accggcctcc gccgcgcgcg ccgcctggat gatgtcgccg acacgcttgc ggatggactg
      181 cgggtcgatg ccgtgctccg cgttgtactc ggtctgcagc cgccggcgcc ggttggtctc
      241 gtcgatcgcg cgcttcatgg agtcggtgag cgagtcggcg tacatgatca cgctgccgtt
      301 cacgttgcgc gccgcgcggc cgatggtctg gatcagggag gtgcccgagc gcaggaagcc
      361 ctccttgtcg gcgtcgagga tggcgaccag cgacacctcc ggcaggtcca ggccctcgcg
      421 cagcaggttg atgccgacca gcacgtcgaa ctcgcccatg cgcagttgcc ggaggatgtc
      481 gatgcgctgc acggtgtcga tctcgctgtg caggtagcgc acgcggacgc ccgactccag
      541 caggtagtcc gtgaggtcct cggacatctt cttggtgagc gtggtgacca gcacgcgctc
      601 gtcgcgctcg gcccgcgcgc gcacctccgc gatgagatcg tcgatctggc ccttggtcgg
      661 ccgcaccacg acctcggggt ccaccagtcc cgtcgggcgg atgacctgct ccacgaccgt
      721 ggtggacacc cgcttctcga acggccccgg cgtggccgac acgtacagcg tctgggggac
      781 gcgcgagcgg aactcctcga acgtcagcgg ccggttgtcc agcgcgctgg gcaggcggaa
      841 gccgtgctcc acgagcgtct gcttgcgcga gcggtccccc tcgtacatgc cgccgatctg
      901 cggcacggtg acgtggctct cgtcgatgac gcagaggaag tcgtcgggaa agtagtccag
      961 cagcgtgaac ggcgcctcgc cgggcgcccg cccgtcgatg tggcgggagt agttctcgat
     1021 gccggagcag aagccgatct cgcgcatcat ctccaggtcg tagttggtgc gcatgcgcag
     1081 gcgctgcgcc tccagcagct tgttctggcc ctccagctcc ttcaggcgct cctccagctc
     1141 gtgctggatg gtgccgatgg cgcgctccgt gcgctggcgg tccgcgacgt agtgggtgtt
     1201 cgggaacacg cccgcctgcg cgacctcctc caccacctcg cccgtcagcg ggtccatgcg
     1261 cgtgatgcgg tccaccgtgt cgccgaagaa ctcgacgcgg taggccagct cgctgtcggc
     1321 cgggaacacc tccagggtgt cgccgcgcag ccggaacttg ccgcgcacga agttgaggtc
     1381 gttgcgctcg tactgcaggt cgaccagctt gcgcagcacc aggtcgagcc cgtggtcctc
     1441 gccctggttc agcatgagca cgtgcttgga gtactcctcc ggcgaaccca ggccgtagat
     1501 gcacgacacc gacgcgacga tcagcacgtc gcggcggctc atgagcgcca tggtggcgcg
     1561 gtggcgcagc cgctccacct cctcgttcac cgagctgtcc ttctcgatgt aggtgtcgct
     1621 ggagggaacg tacgcctcgg gctggtagta gtcgtagtag ctgacgaagt actccaccgc
     1681 gttgcgcggg aagaactcgc ggaactcgtt ggccagctgc gccgccaggg tcttgttcgg
     1741 cgcgatcacc agcgtgggct tctgcaccga ctcgacgatc ttgctggcgg tgaaggtctt
     1801 gccggagccg gtgacgccca gcaggacgac atcggactcc cccgccgcca gcgccttgac
     1861 gacgccctcg atggcccgcg gctggtcccc ggcgggctgg aagtcggacg cgatctcgaa
     1921 ggcgggcatc agcaggccat gctacgtgcc gcctgcgaca ccgaccgcgt cgtcaggggc
     1981 ggcgggacag ctggtgcagc gtcgcggccg cgccggccgc cagcgccgcg ccccccagga
     2041 ccccgcccgg gccgccgccg gtcgcgggca gctccggtcc ggcgccggcc ggggtgagcc
     2101 ggccggtgcc ctccagcacg aacgggtccc gatcgaagta cgtctgcgcg atgtactcgg
     2161 cggtgcgggt ggcgaacatc tgcgcggaga gcgtcgggtt cggcccgccc aggccgtccg
     2221 gcagcgacga cgcgtcgcac acgaacagcc gcttgacctc ccaggactcc tggttgcggt
     2281 ccaccacgct gttgtccggc gtggccgcca tgcgcatcga cgactgcatg tgcaggtaca
     2341 gcggcggcca gtcggcgcgg tgcacgtcgc tcgcccccgc cgcccggagg atgttggcgg
     2401 cctggcggca cagctcgtca cggcggcggt cgctctccgg cgtcggcgtg tactgcacct
     2461 gcgggacggc gccgtgctcg tcgggcgccc agttctgcga cagcgtcacg cggttgtcgc
     2521 ggctgatctc gtcgtcggtg agcaccagca gcgtgaggct gcggtcgtgc tggcgcatgc
     2581 gctcgcgcag cgcgccgccg accacgtggc cgcgcgtgct ccacggctcg ccctggctgt
     2641 tgtcgtccat cgcccaggag ttggagaacg tcatggtgcc gaacgccgtc ttgccggggc
     2701 cgaggcccac cacctccagg cagcccaggc cggggaactc gatgcggctc tggctgttct
     2761 gcccgaccgc cgggtggatg gggaacggga agtagccggt cacgaagtcg aaccagtgca
     2821 gcgtgaggtt gcggccgacc gagtcggtgg agttcggcag gccgctgttc agccacagcc
     2881 gcggggactc gacgcagccc gccgcgagga tcaccacctc ggcggtggcc tcctgcacct
     2941 cgccggagcg cgtgttgcgc cactgcacac cggtcgcggc cggggcgccg tcacgcgtgc
     3001 cggtgaggat cttcgtcgcg aacgcgtcgg tctccagctg gtagcccttg tgctcctcgg
     3061 ccagcgggac gtagctcacg ttggtgccgc gcttggcctt ggcggccagc gcggcgccgt
     3121 gcgggtgcat gcagccctcg tagcagtggc cgcactggtt gcagccggtc ccggcgccga
     3181 agtggcccgg cgggatgatc gcgttgtact gcgggcgcca gccggcgcgg tgcacgtcct
     3241 tgccgttgat ctgcggcagg cccagcttgt gcgcgccgta caccgcccag gcgtccttgg
     3301 tgggcagcgc cgggtcgcgg accaccggca ggatgccctc gacgcgctcg tagtacggca
     3361 ccaggtcggc gtaggcgtgc ggccaggcgc cccgctccac ggccagcggg taggcgcgcg
     3421 gggagttcgc gaagtagtgc tgcgtggtgc cgccgactcc ggagacctgc tgcaccaggc
     3481 cggagccgac catgcggcgc acccacgggc ttcgagagcg gtcggacggc ccccagcgga
     3541 actggccgct gacgacgttg ttcatgtcgg cctcggagtg cgaccagtcg tgctcggggt
     3601 cgcgcgcgta gccgcccgcc tccaggtgca ggatgcgcag gccctgctcg gccagttcct
     3661 tcgcggcgac gggacccccg ccgcccgcgc cgatgatgat gacgtcgtag tcggccatgt
     3721 cgctctccta cggtcccgcg tgtccgcgcg gcctgaagct cgggtcctgc ggccagtcgt
     3781 cctggaagtc gtccaggtgg ccgtgcgacg ggcccgacca gccgatctcc gtccactgcg
     3841 gcatggtgtc gcccggcacg gatgggcgcc ccagcgaggc gtcgccccag tacgcgaagc
     3901 cggcgaacgg caggatctgg ttggcgatct gctggatgtc cggcgacggg tccttgacca
     3961 tgtcgcccag cgtcgccagc cgtccgtcgg tgtcggcctg ggcgaagccc gcgccgtggc
     4021 cgcccttgag cgcgtacgcg tcgagcaccg cggcgacggc cgcggtcacg gagcggtcgg
     4081 gcagcggcgg caacgccttg tcgaactcgg cgatgaccca gcgcgccacc gcgatggtgc
     4141 gctcgtcgcc ctccggcgcg tcgttgacgg cggcgatcag cgcgatgaac gcgcgctcca
     4201 cggtgggcag cgtctgcgcc tgcgcgagct cggtccacgg cgtgcggtag agcacctccg
     4261 gcacgctgag cgcgaacgcg ccgccggcga gcttcaccgc ggcggacaac agctcacggc
     4321 ggttgatcgt cgtcaccccg gccacccttt cgctccggcc cgtgtcccgg cctctttccc
     4381 cggcaggttc gacggcaacg taccggactc ctgccgcgtc agccgccgat gtaggacatc
     4441 tccacgcgcg gcagctgcgg cgcccccgcg gagcgcagct ccgagtagcg atcgtcccgc
     4501 gcgccccaca gccgcgcgac ctcctcgcgc agggcgtcgt cggccgcccc gccgcgcaac
     4561 agcggccgca ggtcgtgacc ggaagccgcg aacaggcagg tgaacacctc gcccaccgcg
     4621 gacagccgcg cgcgcgtgca gtcgccgcag aacggctgcg tcaccgaggc gatcagcccg
     4681 atctcgccgg cgccgtccgc gtagcgccac cgggtggcca cctcgccggg gtaggcgggc
     4741 gccaccggct ccagcggcca gcgcgccgcc aggcgcgcca gcagctccgc cgccggcacc
     4801 acgtcgtcgt ggcgccagcc gttgctggcg cccacgtcca tgtactcgat gaagcgcacc
     4861 gtgacgccgc gctcgcggcc gtacgcggcc agcggcagga tctccgactc gttcacgccg
     4921 cggcgcacca ccgtgttcag cttgacgccg aggcccgcgg cgagtgccgc gtcgatgccc
//
LOCUS       DATMKC010000253         3292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_46520, whole genome shotgun sequence.
ACCESSION   DATMKC010000253 DATMKC010000000
VERSION     DATMKC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46520"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VNU01_07390"
     CDS             complement(<1..750)
                     /locus_tag="VNU01_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWH32479.1"
                     /translation="MAHRSAPILLVVAALLLGGLPSGAARDNGFAPECADAATLSAPT
                     VGAVDCVRLPSEMLGATTAFSYYVPPGCDPALGRDCPVLYYLHGTGGSYREGLGAKGS
                     AGSAWVRALTSGPPVDVRAEAEPWRWANPATWVAKPALDLILVAPHGLTLPGGHGPGP
                     NQNPFWMDWNPRYAQGGDTPRYDTPPPRFESHVIGELIPFVDAWLPTSGDREQRAIVG
                     YSMGGNGAFHIGLKHPDRFASMGMRSGPTLPS"
     gene            842..2008
                     /locus_tag="VNU01_07395"
     CDS             842..2008
                     /locus_tag="VNU01_07395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWH32480.1"
                     /translation="MRHAVIVEAVRTPTGRREGKLKDLHAVDLAAEALKAVVERSGVD
                     AEAVEDVVMGCVSQVGEQSGNIARNAILTAGLPESVCGTTVDRACGSSQQAMHFAAQG
                     VMAGAYDVVIAAGVESMSRVPMGASLSSNGLPFTAAMAQRYADRGGMVMQGISAELIA
                     DHWDLSREELDAFGARSQQRAEAATAEGRFDREIVPVVVETDGVKQELRTDEGIRPGT
                     TAEKLAGLKPSFKPDGKITAGTSSQISDGAAAMLIMSEEAAARHGLTPRARFHTFALA
                     GVDPIFMLTGPIPATQKALERAGLSIGDIDRFEINEAFASVVLAWQRETGADLDRVNV
                     NGGAIALGHPLGCSGVKLMTTLLHELERTGGRYGLQSMCEGGGMANATIIERLG"
     gene            2042..>3292
                     /locus_tag="VNU01_07400"
     CDS             2042..>3292
                     /locus_tag="VNU01_07400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32481.1"
                     /translation="MSIAISEEQLALRDSVTRWAAQRQVAATAREALEAPGDGLPATW
                     PELAAQGWLSVHVPVEHGGGGAGVAELAVVLEALAQAGAPGPVLPTALASALVAAADC
                     GDGPALLSRLVAGEAAAVAWGEGTVQARVAVQPPAGALGAGLVLGAGTAALLVVGAEV
                     EGRTAWFAVDAGGFTAQAPGSLDGAHRVARVALAASVPATRLLAHVDDALVRELAVTL
                     AAAQAAGIAAWCQEAATEYAKVREQFGRPIGSFQAVKHACANLLAKVEMARAAAWDAA
                     HAAADGAQRPLAAAAAGAVALDAAVDCAKTCIQLHGGIGYTWEHDAHLYLRHALVLRQ
                     LLGPTSRYRAEAARGALAGARRSLSLELPAEAEALREGVRAFLAELEGLDADAQRRHI
                     ARHGYVQPHWPAPWGRWASASSPSS"
BASE COUNT          414 a         1216 c         1265 g          397 t
ORIGIN      
        1 cgacggcagc gtcggtccgg agcgcatgcc catgctggcg aagcggtccg ggtgcttgag
       61 gccgatgtgg aacgcgccgt tgccgcccat cgagtagccg acgatggcgc gctgctcgcg
      121 gtcgccggag gtcggcagcc atgcgtcgac gaacgggatc agctcgccga tcacgtgcga
      181 ctcgaagcgc ggcggcggcg tgtcgtagcg cggggtgtcg ccgccctgcg cgtaacgcgg
      241 gttccagtcc atccagaagg ggttctggtt cggtccgggc ccgtggccgc ccggcagcgt
      301 cagcccgtgc ggcgcgacca ggatcaggtc cagcgccggc ttggccaccc aggtggcggg
      361 gttggcccag cgccacggct cggcctccgc gcgcacgtcc accggcggcc cggacgtgag
      421 cgcgcgcacc caggccgaac cggcgctgcc cttggcgccc agcccttcgc ggtaggagcc
      481 gcccgtgccg tgcaggtagt acagcaccgg gcagtcacgt cccagcgcgg ggtcgcagcc
      541 cggcggcacg tagtaggaga acgccgtggt cgccccgagc atctcgctgg gcaggcgcac
      601 acagtccacg gcgcccacgg tgggggcgga cagtgtggcg gcgtccgcgc actccggcgc
      661 gaagccgttg tcgcgggcag cccccgaggg caggccgccg aggagcagcg ccgcgacaac
      721 gagcaggatg ggagcggatc ggtgggccac gtcgcctcca gggcgttgac tgccgtcggg
      781 tcgaacgtta atctccgctc acatacccgc cctgtcaaat ggttttcgtg aggagccccc
      841 catgcgccac gccgtcatcg tcgaagcggt ccgcacgccc acgggcaggc gggagggcaa
      901 gctcaaggac ctgcacgcgg tcgacctggc ggcggaggcg ctcaaggcgg tggtcgagcg
      961 ctcgggcgtg gacgccgagg ccgtcgagga cgtggtcatg ggctgcgtgt cccaggtggg
     1021 ggagcagtcc ggcaacatcg cgcgcaacgc gatcctcacc gcgggcctgc cggagtccgt
     1081 gtgcggcacc acggtcgacc gcgcctgcgg ctcctcccag caggccatgc acttcgccgc
     1141 ccagggggtc atggccggcg cctacgacgt cgtgatcgcc gcgggcgtgg agtccatgag
     1201 ccgcgtgccg atgggcgcgt cgctgtcgag caacggcctg ccgttcacgg ccgccatggc
     1261 gcagcgctac gccgaccgcg gcggcatggt catgcagggc atctccgccg agctcatcgc
     1321 cgaccactgg gacctgtcgc gggaggagct cgacgcgttc ggggcgcgca gccagcagcg
     1381 ggccgaggcc gccaccgccg agggccgctt cgaccgcgag atcgtgcccg tggtggtgga
     1441 gaccgacggt gtcaagcagg agctgcgcac cgacgagggc atccgcccgg gcaccacggc
     1501 ggagaagctc gccgggctca agccctcttt caagccggac ggcaagatca cggccgggac
     1561 gtcctcgcag atcagcgacg gcgccgccgc gatgctgatc atgagcgagg aggccgccgc
     1621 gcggcacggg ctcacgcccc gcgcgcgctt ccacacgttc gcgctggccg gcgtggaccc
     1681 gatcttcatg ctgaccggcc ccatccccgc cacgcagaag gcgctggagc gcgcggggct
     1741 gtccatcggc gacatcgacc gcttcgagat caacgaggcg ttcgcctccg tggtgctcgc
     1801 ctggcagcgc gagacgggcg cggacctcga ccgggtgaac gtcaacggcg gcgcgatcgc
     1861 gctcggccac ccgctgggct gctccggcgt gaagctcatg accacgctgc tgcacgagct
     1921 ggagcggacg ggtgggcgct acggcctgca gagcatgtgc gagggcggcg gcatggccaa
     1981 cgccaccatc atcgagcggc tgggctgagg cccgcgccca cgcgaccagg agccctgtcc
     2041 catgtcgatc gccatctccg aggaacagct cgccctgcgc gacagcgtca cccgctgggc
     2101 ggcgcagcgc caggtcgcgg cgaccgcgcg cgaggcgctg gaggcgcccg gcgacgggct
     2161 gccggccacg tggccggagc tggccgcaca gggctggctc agcgtgcacg tgcccgtcga
     2221 gcacggcggc ggcggcgccg gcgtcgccga gctggcggtg gtgctggagg cgctggcgca
     2281 ggccggtgcg cccggacccg tgctgcccac ggccctggcg tccgcgctcg tcgccgccgc
     2341 cgactgcggt gacggcccgg cgctgctgtc gcgcctggtc gcgggcgagg ccgccgccgt
     2401 ggcttggggc gagggcacgg tgcaggcccg cgtcgccgtc cagccgccag ccggcgccct
     2461 cggcgcgggc ctggtgctcg gcgcgggcac cgcggcgctg ctcgtcgtgg gcgcggaggt
     2521 cgagggccgc acggcgtggt tcgccgtcga cgccggcggc ttcaccgcgc aggcgcccgg
     2581 cagcctcgac ggcgcccacc gcgtggcgcg ggtcgccctt gccgcgtcgg tgccggccac
     2641 gcggctgctg gcccacgtcg acgacgcgct ggtgcgcgag ctcgccgtga ccctggcggc
     2701 cgcccaggcc gcgggtatcg cggcgtggtg ccaggaggcc gcgaccgagt acgccaaggt
     2761 gcgcgagcag ttcgggcggc ccatcggctc gttccaggcc gtcaagcacg cctgcgccaa
     2821 cctgctcgcc aaggtcgaga tggcccgggc ggcggcgtgg gacgccgcgc acgcggccgc
     2881 ggacggcgca cagcggcccc tggcggccgc cgcggccggg gccgtggcgc tggacgccgc
     2941 ggtcgactgt gccaagacct gcatccagct gcacgggggc atcggctaca cctgggagca
     3001 cgacgcccac ctgtacctgc gccacgcgct ggtcctgcgc cagctgctcg gccccacgtc
     3061 ccgctaccgc gccgaggcgg cgcgaggcgc gctggccggc gcccgccgca gcctgtcgct
     3121 cgagctgccc gccgaggccg aggcgctgcg cgagggcgtg cgcgccttcc tggcggagct
     3181 ggagggactg gacgccgacg cccagcgccg ccacatcgcc cgccacggct acgtgcagcc
     3241 gcactggccg gcgccgtggg ggcgctgggc gtcggcgtcc agtccctcca gc
//
LOCUS       DATMKC010000254         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_176848, whole genome shotgun sequence.
ACCESSION   DATMKC010000254 DATMKC010000000
VERSION     DATMKC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176848"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..>2676
                     /gene="uvrA"
                     /locus_tag="VNU01_07405"
     CDS             550..>2676
                     /gene="uvrA"
                     /locus_tag="VNU01_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWH32482.1"
                     /translation="MPKARPTRQHADHLVVRGAREHNLRNVDLEIPRDALVVFTGLSG
                     SGKSSLAFDTIYAEGQRRYVESLSAYARQFLGMMEKPEVDFIEGLSPAISIDQKSTSR
                     NPRSTVGTITEVYDYLRLLYARVGKPHCPECGKPISRQTAEQIIDQVEQLPEGTKFQV
                     LAPVVRGRKGNYEKVLGDLSSRGYARARITSGTAAPQVVDLATPPELKRYEQHTIEVI
                     VDRLVAKPGIRRRLADSIETALQLAEGIASVEIVGGDEPGEVLTFSEHLACPDCGLSF
                     DQLAPRNFSFNTPYGACDVCSGLGTRLEVDAELVVGDEDLSLEDGVLLPWSSGSQSTY
                     YEQLLRAVADHLGIKPGTPWRKLKAEHKRAILHGLPGGVQVTVRYTNRYGRPRQYRAN
                     FEGVISQLYRRHSESESDYVREKIQEYMREVPCSACHGARLKPLVLAVKIEGKSIAEA
                     AALSIGEADRFFGELELNERDTMIADSILKEVRARLRFLLDVGLEYLTLDRAAATLAG
                     GEAQRIRLATQIGSGLVGCLYVLDEPSIGLHQRDNERLIETLVRLRDLGNTVIVVEHD
                     LATIEAADHIVDIGPGAGEHGGDIVHSGDLAGKREIAVPARRRKPQRGHELVVRGARE
                     HNLTGIDVRFPLSTFVAVTGVSGSGKSTLVNDILHAVLTREINGSRVVPGKHKRVDGL
                     DRIDKVVGVDQSPIGRTPRSNPATYTG"
BASE COUNT          446 a          995 c          908 g          327 t
ORIGIN      
        1 tggacaacct cgtcctggtc tgctggcgct gccacagcca catccaccaa cacggctggc
       61 aagtgtggaa ccacaacggc cgctacaccg ccgggccacc aggccaagca ccaccgcacc
      121 ttgaaaacaa catagagaaa ccagcgctcg cccataccgg ctgacccgcc accccacccg
      181 ccaggtgggg cgcgaagccc gcccacacac cgccaacagg cgggcggggc ggtggtcggc
      241 gtgggaaccg gagcccgacg aggacgcgcc cggctcgcgg catcacgcac gcatggccgc
      301 aacatggccg ccgacgcccg acaccgccac ataagccggg cgaggagcga ggaggggtcc
      361 gggcccacgc gaccgcgcac acgcaccgcg gcatcagccg ggcgaggagc gaggaggggt
      421 ctcggcccac gcgaccaccg acacgcaccg atacgcgcgg gcctcggacg gtgtcgcacc
      481 ccctcgatag actggcccgc tgagggccgt gcgggccctg ttcccccgtg aatccggagc
      541 gtttccgccg tgccgaaggc ccgaccgacc cgccagcacg cggaccacct ggtggtccgc
      601 ggcgcccggg agcacaacct cagaaacgtc gatctggaga tcccgcgcga cgcgctggtg
      661 gtcttcaccg gcctgtccgg gtccggcaag tccagcctcg ccttcgacac catctacgcc
      721 gagggccagc gccgctacgt ggagtccctg tcggcctacg cccggcagtt cctcggcatg
      781 atggagaagc cggaggtcga cttcatcgag ggcctgtcgc cggccatctc catcgaccag
      841 aagtcgacca gccgcaaccc gcgctccacg gtcggcacca tcacggaggt gtacgactac
      901 ctgcgcctgc tgtacgcgcg cgtcggcaaa ccgcactgcc cggagtgcgg caagccgatc
      961 agccggcaga ccgccgagca gatcatcgac caggtcgagc aactgccgga gggcaccaag
     1021 ttccaggtcc tcgcgccggt ggtgcgcggg cgcaagggca actacgagaa ggtgctcggc
     1081 gacctgtcca gccgggggta cgcccgcgcg cgcatcacgt ccggcaccgc cgcgccgcag
     1141 gtggtggacc tggccacgcc gccggagctc aagcgctacg agcagcacac catcgaggtg
     1201 atcgtcgacc ggctggtggc caagccgggc atccgccgcc gcctggccga ctccatcgag
     1261 acggcgctgc agctcgccga gggcatcgcc tcggtggaga tcgtcggcgg ggacgagccg
     1321 ggcgaggtgc tcaccttctc cgagcacctg gcgtgccccg actgcggcct gagcttcgac
     1381 cagctcgcgc cccgcaactt ctccttcaac accccctacg gcgcctgcga cgtctgctcg
     1441 gggctcggca cccgcctgga ggtcgacgcg gagctggtgg tcggcgacga ggacctgtcg
     1501 ctggaggacg gcgtgctgct gccgtggtcc agcggcagcc agtcgaccta ctacgagcag
     1561 ctcctgcgcg ccgtggccga tcatctgggc atcaagcccg gcacgccgtg gcgcaagctc
     1621 aaggccgagc acaagcgcgc gatcctgcac gggctgcccg gcggcgtgca ggtcaccgtg
     1681 cgctacacca accgctacgg gcgcccgcgc cagtaccggg cgaacttcga gggcgtgatc
     1741 tcgcagctgt accggcgcca ctcggagagc gagtcggact acgtccgcga gaagatccag
     1801 gagtacatgc gcgaggtgcc gtgctcggcc tgccacggcg cgcgcctcaa gccgctggtg
     1861 ctggccgtga agatcgaggg caagtcgatc gccgaggcgg ccgcgctgtc catcggcgag
     1921 gccgaccgct tcttcggcga gctggagctc aacgagcgcg acacgatgat cgccgacagc
     1981 atcctcaagg aggtgcgcgc ccgcctgcgc ttcctgctgg acgtcggcct ggagtacctg
     2041 acgctggacc gggcggccgc cacgctcgcg ggcggcgagg cgcagcgcat ccgcctggcc
     2101 acccagatcg gctccggcct ggtcggctgc ctgtacgtgc tggacgagcc gtccatcggc
     2161 ctgcaccagc gcgacaacga gcgcctcatc gagacgctcg tgcgcctgcg cgacctgggc
     2221 aacaccgtca tcgtcgtgga gcacgacctc gccaccatcg aggccgccga ccacatcgtc
     2281 gacatcggcc ccggcgcggg cgagcacggc ggcgacatcg tccactccgg cgacctggcc
     2341 ggcaagcgcg agatcgccgt gcccgccagg cgccgcaagc cgcagagggg ccacgagctg
     2401 gtggtgcggg gcgcgcggga gcacaacctc accgggatcg acgtgcgctt tccgctgtcc
     2461 acgttcgtcg ccgtcaccgg cgtcagcggc agcggcaagt cgaccctggt caacgacatc
     2521 ctgcacgccg tgctgacgcg ggagatcaac ggcagccggg tggtgccggg caagcacaag
     2581 cgcgtcgacg ggctggaccg catcgacaag gtggtggggg tcgaccagtc gccgatcggc
     2641 cgcacgccgc gctccaaccc ggccacgtac accggt
//
LOCUS       DATMKC010000255         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_192936, whole genome shotgun sequence.
ACCESSION   DATMKC010000255 DATMKC010000000
VERSION     DATMKC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192936"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VNU01_07410"
     CDS             complement(<1..605)
                     /locus_tag="VNU01_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWH32483.1"
                     /translation="MAKNLVIVESPTKAKTIEKYLGKDYKVLASVGHIRDLPRSNFAI
                     EVDNGDVHLVYEVPKTAAKHVSAIKTAAKSAERVFLATDLDREGEAIAWHVAEVAGID
                     TDEANRVVFAEITKDAILAAFSTPRPIDRNLVDAQQARRAVDRIVGYRLSPTLWRNVA
                     SGISAGRVQSVALRLLGVHQVAVDRARRGERRQDRVLGDLG"
     gene            complement(699..1697)
                     /locus_tag="VNU01_07415"
     CDS             complement(699..1697)
                     /locus_tag="VNU01_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWH32484.1"
                     /translation="MDAPSPFLDRTRQVRELAERDLAPAAARAAESKGRDRPEEADPY
                     RTAFERDRDRILHSKAFRRLKHKTQVFIQPEGDHTMTRLTHALQVTQIARALASALGL
                     NEALAEAIALGHDVGHSPFGHIGEEALEELVPGGWHHAAQSVRIYEVLEPENLTWEVR
                     DGIRAHSWKIDPPPATLEGGVVRFADRIAYLSHDVLDSARVGVLDLADLPERARKVLG
                     DEPGSAWIHRMIHSVVQESLRTGRLVMEPEMAEAMDELRDFMFAAVYQAEHREDEQRQ
                     AKLVVRQLAEYHLEHPALLPPTYRDTAADPLTQVVDFVAGMTDRYAMALHDRLLRR"
     gene            complement(1750..2307)
                     /locus_tag="VNU01_07420"
     CDS             complement(1750..2307)
                     /locus_tag="VNU01_07420"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWH32485.1"
                     /translation="MGHDTARRWTAEEFLALPEDDRRIELFDGVIVVTPSAVSRHELV
                     IARISFALMAQVDPLGGVVFGSSLDTRIDDRNVLQPDVQVFLPGHVHRIGPKFVEGPA
                     DLVAEVSSPKTKGRDRVRKRALYEGFGVAEFWIVDLDEDVIEVYRLHDGGYAAPQRLG
                     HGDTVTTPLLPGWSAEVDWLLPDLP"
BASE COUNT          353 a          909 c          849 g          387 t
ORIGIN      
        1 tcgccgagat caccaaggac gcgatcctgg cggcgttctc cacgccgcgc ccgatcgacc
       61 gcaacctggt ggacgcccag caggcgcagc gccacggact gcacgcggcc ggcggagatc
      121 ccgctggcga cgttgcgcca cagcgtgggc gacagccggt agccgacgat gcggtccacg
      181 gcgcggcgcg cctgctgggc gtccaccagg ttgcggtcga tcgggcgcgg cgtggagaac
      241 gccgccagga tcgcgtcctt ggtgatctcg gcgaagacga cccggttggc ctcgtcggtg
      301 tcgatgccgg ccacctcggc gacgtgccag gcgatcgcct cgccctcacg gtcgaggtcg
      361 gtggcaagga agacgcgctc ggcggacttc gcggccgtct tgatcgcgct cacgtgcttc
      421 gccgcggtct tgggcacctc gtagaccagg tgcacgtcgc cgttgtcgac ctcgatcgcg
      481 aagttgctgc gcgggaggtc tcggatatgg ccgaccgagg cgaggacctt gtagtccttg
      541 cccaggtact tctcgatcgt cttcgccttc gtgggcgact cgacgatcac caggttcttg
      601 gccatgagtg tctttggata ccccctcgcc gtgcggcgac cgccggcggg atgtcgggaa
      661 agcagagcgc gcggatcacg ttatccggcg ccccgacctt agcggcgcaa cagccgatcg
      721 tgcagcgcca tggcgtaacg gtccgtcatg ccggccacga agtcgaccac ctgggtcagc
      781 gggtccgccg ccgtgtcgcg gtaggtcggc ggcagcaacg ccgggtgctc caggtggtat
      841 tccgccagct gccggaccac cagcttggcc tggcgctgct cgtcctcacg gtgctcggcc
      901 tggtagacgg ccgcgaacat gaagtcgcga agctcgtcca tcgcctcggc catctcgggc
      961 tccatcacca gccggccggt gcgcaggctc tcctgcacca cggaatggat catccggtgg
     1021 atccacgccg agccgggctc gtcgcccagc accttgcgcg cccgctccgg cagatccgcc
     1081 aggtccagca cgccgacccg cgcggagtcc agcacgtcat gggacaggta ggcgatgcgg
     1141 tcggcgaagc gcaccacgcc gccctccagc gtggccggcg gcgggtcgat cttccacgag
     1201 tgcgcgcgga tgccgtcgcg cacctcccag gtgaggttct ccggttcgag cacctcgtag
     1261 atgcgcacgc tctgcgcggc gtggtgccag ccgcccggca ccagctcctc cagcgcctcc
     1321 tcgccgatat gcccgaacgg cgagtggccc acgtcgtggc ccaacgcgat ggcctcggcg
     1381 agcgcctcgt tgagcccgag cgcggatgcc agcgcccgcg cgatctgggt cacctgcagc
     1441 gcgtgggtca gccgcgtcat cgtgtggtcg ccctcgggct ggatgaacac ctgcgtcttg
     1501 tgcttgagcc gccggaacgc cttggagtgc aggatccggt cgcggtcgcg ctcgaacgcc
     1561 gtgcggtacg ggtccgcctc ctccggccgg tcgcgtccct tggactccgc cgcgcgcgcg
     1621 gcggccggcg cgaggtcgcg ctcggcgagc tcgcgcacct gccgcgtgcg gtccaggaaa
     1681 ggtgaggggg cgtccatcac ctcgacggta acaccacctc gcgacaccgc ccgctcagcg
     1741 cggcgcggtt cagggcaggt ccggcaggag ccagtccact tcggcgctcc agccgggcag
     1801 aagcggcgtc gtgacggtgt caccgtgacc cagacgctgc ggtgccgcgt agccgccgtc
     1861 gtggaggcga tagacctcga tgacgtcctc gtcgaggtcc acgatccaga actccgccac
     1921 gccgaacccc tcgtacaacg cgcgtttgcg cacccggtcc cggcccttcg tcttcggaga
     1981 gctgacctcc gcgacgagat cggcgggccc ctcgacgaac ttcggaccga tgcggtgcac
     2041 gtggccgggc aggaacacct ggacgtccgg ctggagcacg ttgcggtcgt cgatgcgggt
     2101 gtccaggctg gagccgaaca ccacgccgcc cagcggatcc acttgcgcca tgagcgcgaa
     2161 gctgatcctc gcaatcacca gttcgtggcg cgaaaccgcg gacggcgtca caacgatgac
     2221 cccgtcgaac agttcgatgc gccggtcgtc ctcgggcagc gcgaggaact cctcggccgt
     2281 ccagcgccga gccgtgtcgt ggcccatgcc cgtcctcctc ccgtcatggc gcgaaaccgt
     2341 agtcatcgtg cgccgatcgc gagccggatc gccagccctg cttgtggaca accgagcgcg
     2401 cccgtgcggc ctggctttga gccagggccg tgccctccta cactgcctgc cgaacacccc
     2461 cttgtcccgc ggcgggctgc cgtgcgcgcc cggaaccc
//
LOCUS       DATMKC010000256         4487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_352184, whole genome shotgun sequence.
ACCESSION   DATMKC010000256 DATMKC010000000
VERSION     DATMKC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352184"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VNU01_07425"
     CDS             <1..1298
                     /locus_tag="VNU01_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWH32486.1"
                     /translation="WVSVALSELDRELAAQHDGKPFTVQTDGGDFRLRLLDVGDRYGA
                     YYNDVANRLLWFTLHGLWGEPYEPSGAGWREPWERAYVPVNDLVADAVADAAENGAEV
                     FLQDYHLTVAPGFVRERLPRAPILQYVHTPWPAPALLRRLPDAMTREILRALLSADVV
                     GFSSPAWCRAFRHCAVELLGAASHGDVVEHAGGRTVVADFVLGVDADALRESAREPAT
                     QAAAARLEAELDGRRLILRVDRTDLSKNILRGLLAYELLLERHPEHRGRVWHYAHLNP
                     SRQSVPEYQRYLAACQDAAARIQDRFGERAVRLFVGDDYATAVAALRRYDVLLANPVT
                     DGTNLVAKEGPVLNAREGALVLSRNAGAADVLHGGALVINPYDVEETADALHRALRMP
                     TRERTERAALLRQAAVVGAPHEWFHAQRQLLRAVVARRR"
     gene            complement(1301..2089)
                     /gene="otsB"
                     /locus_tag="VNU01_07430"
     CDS             complement(1301..2089)
                     /gene="otsB"
                     /locus_tag="VNU01_07430"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWH32487.1"
                     /translation="MRSAEEAIDLLVAHGRRAGLFLDFDGTLAPIVADPDTSAMPPEL
                     APLLTEVATALGVLAVVSGRPAAFLAERVRVDGAALLGLYGLETWDGRMAVPRPDATE
                     WRPRVREGLNRLRAALDGADGIWVEDKGLAVAVHWRNAADRPDAEREVGLLVEALSAE
                     TGLAHVPGKLVAELRPPVRWDKGDAVRALSADRGLAVAVYVGDDLGDLPAFAAAREHG
                     GMGVAVSGGEETPPELLARADAALDGPGGLRVWLEVLRERLTGA"
     gene            complement(2076..2873)
                     /locus_tag="VNU01_07435"
     CDS             complement(2076..2873)
                     /locus_tag="VNU01_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWH32488.1"
                     /translation="MARVPRDHSADAGGDPAVLDAKRALREEVWDALAASGAARFPGA
                     RHRIPNFTGAEAAAQRLRATEAWRRARVVKANPDSPQWPVRQRALEDGLLLAMAVPRL
                     AEPEPFFLLDPGELDGGYRAASSIKGASAHARTVAVAELEPFDLIVTGCVAVDRSGAR
                     LGKGGGFADLEFGLLAEAGLVGPSTVVATTVHPVQVLEPGRIPMGAHDAPLDLVVTPD
                     EVIVCDGGRARPDGIAWDQLTREKIDAIPLLRALCPTDSPAGDHAKR"
     gene            complement(2873..3541)
                     /gene="miaE"
                     /locus_tag="VNU01_07440"
     CDS             complement(2873..3541)
                     /gene="miaE"
                     /locus_tag="VNU01_07440"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HWH32489.1"
                     /translation="MSGAPRLPSGAEPRPLTAPWPEYLPDVLGVATPPGWQDVALAGL
                     PATMSDHAHAEKKAALSALSLLSREPSRTDLVDRMGKLALEELGHLDRVLAHLRRRGW
                     TLQPDRPDRYANRLLGLRRAGGDEALMDRLLVSALIEARSWERLHLLASALDGARAVV
                     DGGEELSAFYTELAHSEAGHYRLFVQLAEQECPGQDVRGRLGELAAAEAEIVAALPHE
                     PRIH"
     gene            complement(3538..3810)
                     /locus_tag="VNU01_07445"
     CDS             complement(3538..3810)
                     /locus_tag="VNU01_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32490.1"
                     /translation="MEVFDTLAGEAAWKALESGATYQHARERREQGALVWRIDLFAEV
                     VRPQAAVVVLVAPGSDTDVAAGVEVKADVGMLGSRRHPSEPVEWGR"
     gene            complement(3810..>4487)
                     /locus_tag="VNU01_07450"
     CDS             complement(3810..>4487)
                     /locus_tag="VNU01_07450"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HWH32491.1"
                     /translation="LRTIRRAFGGTINDIVVSVCGDAVGRLLRWRGYETKDLDLRVMV
                     PVRVHGEGEVPGSVQELGQGMGQTQSVGGGVVGVMAPLPVMQMDPVARLYRIMGEMAG
                     LKESRQAVAADSLMRLAGYAPPNLHALAARLASGQARYNLALSNAPGPQEPRYLHGVP
                     MEGSFPFIPLAGDCALSIALTSYAGRMYVGLVGDWDGMPDLDTLSEFLAEAVAELLDA
                     AQEQAAR"
BASE COUNT          555 a         1832 c         1566 g          534 t
ORIGIN      
        1 cgtgggtgtc cgtggcgctg tcggagctgg accgcgagct ggccgcccag cacgacggca
       61 agccgttcac cgtgcagacc gacggcggcg acttccgcct gcgcctgctg gacgtgggcg
      121 accgctacgg cgcctactac aacgacgtcg ccaaccgcct gttgtggttc acgctgcacg
      181 gcctgtgggg cgagccgtac gagcccagcg gcgccggctg gcgcgagccg tgggagcgcg
      241 cctacgtccc cgtgaacgac ctcgtggccg acgccgtggc cgacgccgcc gagaacggcg
      301 ccgaggtgtt cctgcaggac taccacctca ccgtcgcgcc cggcttcgtg cgcgagcgcc
      361 tgccgcgcgc gccgatcctg cagtacgtcc acacgccgtg gcccgcgccg gccctgctgc
      421 ggcgcctgcc cgacgcgatg acgcgcgaga tcctgcgcgc gctgctgtcg gccgacgtcg
      481 tggggttctc ctcccccgcg tggtgccgcg cgttccgcca ctgcgccgtg gagctgctgg
      541 gcgccgcctc ccacggcgac gtggtggagc acgccggcgg gcgcacggtg gtcgccgact
      601 tcgtgctcgg cgtcgacgcc gacgcgctgc gcgagtccgc gcgggagccg gccacgcagg
      661 ccgccgccgc gcggctggag gccgaactgg acgggcgccg gctgatcctg cgcgtggacc
      721 gcacggactt gtccaagaac atcctgcgcg gcctgctggc ctacgagctg ttgctggagc
      781 gccaccctga gcaccgcggg cgcgtgtggc actacgccca cctcaacccg tcacgccagt
      841 ccgtgcccga gtaccagcgc tacctggccg cctgccagga cgcggccgcg cgcatccagg
      901 accgcttcgg cgagcgcgcc gtccggctgt tcgtgggcga cgactacgcc acggccgtcg
      961 ccgcgctgcg ccgctacgac gtcctgctgg ccaatcccgt caccgacggc accaacctgg
     1021 tggccaagga aggcccggtg ctgaacgcgc gcgagggtgc cctggtgctg tcgcgcaacg
     1081 ccggcgcggc cgacgtgctc cacggcggcg ccctcgtcat caacccctac gacgtcgagg
     1141 agaccgccga cgcgctgcac cgggcgctgc gcatgccgac gcgcgagcgc accgagcgcg
     1201 ccgcgctgct gcgccaggcc gccgtggtcg gggcgccgca cgagtggttc cacgcgcagc
     1261 gccagctcct gcgcgccgtg gtcgcccgcc gccgctgaac tcaggcgccg gtgaggcgct
     1321 cgcgcaggac ctccagccac acgcgcaggc cccccggccc gtcgagcgcg gcgtcggccc
     1381 gggcgagcag ctcgggcggc gtctcctccc cgcccgacac agccacgccc atgccgccgt
     1441 gctcccgcgc cgcggcgaac gccggcaggt cgccgaggtc gtcgcccacg tacacggcca
     1501 ccgcaaggcc gcgatccgcg ctgagcgccc gcacggcgtc gcccttgtcc cagcgcaccg
     1561 gcggccgcag ctcggcgacc agcttccccg ggacgtgcgc cagaccggtc tcggccgaca
     1621 gcgcctccac cagcagcccg acctcccgct ccgcgtcagg acggtcggcg gcgttgcgcc
     1681 agtgcaccgc gacggccagg cccttgtcct ccacccagat gccgtcggcg ccgtccaggg
     1741 cggcgcgcag gcggttgagc ccttcgcgga cgcggggccg ccactccgtg gcgtcgggcc
     1801 gtggcacggc catgcggccg tcccaggtct ccagcccgta caggcccagc agcgccgcgc
     1861 cgtcgacccg cacgcgctcg gccaggaacg ccgcgggcct gccgctcacc acggccagga
     1921 cgccgagcgc cgtcgcgacc tcggtgagca gcggcgccag ctccggcggc atcgccgagg
     1981 tgtccgggtc cgccacgatc ggcgcgagcg tcccgtcgaa gtccaggaac agccctgccc
     2041 gccgcccgtg ggcgaccagc aggtcgatgg cctcctcagc gcttcgcatg gtctccagca
     2101 ggtgagtcgg tggggcacag cgcccgcagc agcggaatgg cgtcgatctt ctcccgcgtg
     2161 agctgatccc acgcgatgcc gtccgggcgc gcccgcccgc cgtcgcacac gatgacctcg
     2221 tcgggcgtga ccaccaggtc caggggcgcg tcgtgcgcgc ccatcgggat gcgccccggc
     2281 tccagcacct gcaccgggtg caccgtggtg gccaccacgg tcgacgggcc gaccaggccc
     2341 gcctcggcca gcaggccgaa ctccaggtcc gcgaagccgc cgcccttgcc caggcgcgcg
     2401 ccgctgcggt ccaccgccac gcagcccgtc acgatcaggt cgaacggctc cagctccgcc
     2461 acggccacgg tgcgcgcgtg cgccgaggcc cccttgatgg acgacgcggc gcggtagccg
     2521 ccgtccagct cccccgggtc cagcaggaag aacggctcgg gctctgcgag gcgcggcacc
     2581 gccatggcca gcagcaggcc gtcctccagc gcgcgctggc gcaccggcca ctgggggctg
     2641 tccgggttcg ccttgaccac gcgggcgcgc cgccacgcct ccgtggcgcg cagccgctgt
     2701 gccgcggcct ccgcgccggt gaagttgggg atgcggtggc gcgcgcccgg gaagcgcgcc
     2761 gcgcccgagg ccgccagcgc gtcccagacc tcctcgcgca gcgcccgctt cgcgtccagc
     2821 acggccggat cgccaccggc gtcggcgctg tggtcccggg gcacgcgcgc catcagtgga
     2881 tccgcggctc gtgcggcagg gcggcgacga tctccgcctc cgcggcggcc agctcaccca
     2941 gccgcccccg cacgtcctgc cccgggcact cctgctccgc aagctgcacg aacaggcggt
     3001 agtggccggc ctcggagtgc gccagctccg tgtagaacgc gctcagctcc tccccgccgt
     3061 ccaccacggc gcgcgcgccg tccagcgccg acgccagcag gtgcagccgc tcccaggagc
     3121 gcgcctcgat cagcgccgac accagcagcc ggtccatcag cgcctcgtcc ccgccggcgc
     3181 gccgcaggcc gagcaaccgg ttggcgtagc ggtcgggccg gtccggctgg agcgtccagc
     3241 cgcgccgccg caggtgcgcc agcacgcggt cgaggtggcc cagctcctcc agcgccagct
     3301 tgcccatgcg gtccaccagg tcggtgcgcg acggctcgcg cgacagcagc gacagcgcgg
     3361 acagggccgc cttcttctcc gcgtgcgcgt ggtcgctcat cgtcgccgga aggccggcca
     3421 gggcgacgtc ctgccagccc ggcggcgtcg ccacgcccag cacgtccggc aggtactccg
     3481 gccacggcgc cgtcagcggc cggggctcgg cgcccgaggg caggcgcggc gcgccgctca
     3541 ccggccccac tccaccggct ccgaggggtg gcggcggctg ccgagcatgc cgacgtccgc
     3601 cttcacctcg acaccggcgg ccacgtcggt gtcgctgcct ggcgcgacca gcaccacgac
     3661 ggcggcctgc gggcgaacca cctcggcgaa caggtcgatg cgccacacca gcgcaccctg
     3721 ttccctgcgt tcccgggcgt gctggtacgt ggcgccgctc tccagcgcct tccacgccgc
     3781 ctctcctgcc agcgtgtcga acacctccat cagcgggcgg cctgctcctg cgccgcgtcg
     3841 aggagctccg cgaccgcctc ggccaggaac tcgctcagcg tgtccaggtc cggcatgccg
     3901 tcccagtcgc ccaccaggcc gacgtacatg cgcccggcgt aggacgtgag cgcgatcgac
     3961 agcgcgcagt cgcccgccag cgggatgaac ggaaagctgc cctccatcgg cacgccgtgc
     4021 aggtagcgcg gctcctgcgg gccgggcgcg ttggacagcg ccaggttgta gcgggcctgc
     4081 ccggaagcca ggcgcgccgc cagcgcgtgc aggttcggcg gcgcgtaacc cgccaggcgc
     4141 atcagcgagt cggcggccac ggcctggcgg gactccttca gcccggccat ctcccccatg
     4201 atccggtaca gccgcgcgac cgggtccatc tgcatgacgg gcagcggcgc catgacgcca
     4261 accacaccgc cgccgacgct ctgcgtctgg cccatgccct ggcccagctc ctgcacgctg
     4321 ccgggcacct cgccctcgcc gtgcacgcgc accgggacca tgacgcgcag gtccaggtcc
     4381 ttggtctcgt agccgcgcca gcgcagcagc cggccgaccg cgtcgccgca caccgacacc
     4441 acgatgtcgt tgatggtccc gccgaacgcg cggcggatcg tgcgcag
//
LOCUS       DATMKC010000257         7051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_149228, whole genome shotgun sequence.
ACCESSION   DATMKC010000257 DATMKC010000000
VERSION     DATMKC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149228"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VNU01_07455"
     CDS             <1..926
                     /locus_tag="VNU01_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWH32492.1"
                     /translation="LLARPFRAFALLFRPLIRLLNATANAIVRALGVEPQDELAMTHS
                     PADLLLLLEESSEQGTLASEQHDLLSRALELGGLDAQSVMVPRRDVVAVSAEADAGEI
                     EELARATGRSRLPVYEDDLDRIRGVLHVKDLLVLDADERERVRAGALARPALVVPESR
                     PLEALMVDMRQRRQHVALVIDEYGSVSGLVALEDLIEELIGEFEDESDRRARVFRRRA
                     DGAYLVPASLRPDEVEERLGVELPDGDWETMAGLVIAHLERLPEVGDVAVADGVRLEV
                     TRVDAHRLIELALTPRPDPTGNGGHPEIGAS"
     gene            complement(1022..1300)
                     /locus_tag="VNU01_07460"
     CDS             complement(1022..1300)
                     /locus_tag="VNU01_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32493.1"
                     /translation="MMVARLVIEDDTDAVAPGVAASLAVLAGIAASDAACCARLKVRA
                     RGTDHQEAVALLAGVEPHGKRMARDLDRILKRKDDAHYGLTLLPVPTR"
     gene            complement(1363..1926)
                     /locus_tag="VNU01_07465"
     CDS             complement(1363..1926)
                     /locus_tag="VNU01_07465"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWH32494.1"
                     /translation="MIPSLDGPVLAALAGTTQPLSLTRVRAVAGRGSLAGVRRVLLRL
                     VDAGLAEAVPGGYVLNREHVAAPAVVALAGLHGELSARLRGAVAQWDGDCQLLGLFGS
                     AARRDGDETSDIDVLLVSPSGDARDFAEALAADVERWTGNHGHIVVVTPAQLEGMRRV
                     DEPILESWRRDLIVVLGSRSALDGAAS"
     gene            complement(2147..3313)
                     /gene="moeB"
                     /locus_tag="VNU01_07470"
     CDS             complement(2147..3313)
                     /gene="moeB"
                     /locus_tag="VNU01_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWH32495.1"
                     /translation="MEPLIAEPASLTNEEIQRYARHLIMPNVGVDGQGRLKNAKVLLV
                     GTGGLGSPLAMYLAAAGVGTIGLVDFDVVDLSNLQRQVIHGTSDIGKRKVVSAMESIK
                     DINPNVEVVIHETALLSENALEIIAPYDLVIDGTDNFPTRYLVNDACVLLGKPNVYGS
                     IFRFDGQLSVFWAEHGPCYRCMFPEPPPPGMVPSCAEGGVFGVICATIGAGQATEGIK
                     LITGIGEPLIGRMQIYDALENRWQTIKVNKDPECPVCGKNPTVTELIDYEEFCGVPAN
                     DHESTGAGEPEELEVTPQEYAEMAERVKLLDVREPHEFQISRIAGAELIPLAELPDSL
                     QDLELNAEYVVLCKSGARSLRAAELMREAGLRARSMRGGINAYARTVDPSIPTY"
     gene            complement(3372..3815)
                     /locus_tag="VNU01_07475"
     CDS             complement(3372..3815)
                     /locus_tag="VNU01_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HWH32496.1"
                     /translation="MSDAALQGWPIEPEPGTPEYDAAQAQAQVQVQAFADVPLDDDGL
                     PTDEAVRTALKAVLDPEIGINIVDLGLVYGVEKEPPGKVKLTMTLTSLGCPLTELIHQ
                     QCTMILTRLPGVEDVDVNFVFSPPWSTEMIAPEAKDELRAMGFSV"
     gene            complement(3808..4269)
                     /locus_tag="VNU01_07480"
     CDS             complement(3808..4269)
                     /locus_tag="VNU01_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWH32497.1"
                     /translation="MSLDDLYHEIILDHYRSPRHRAAELEGAQVHVHHSNPLCGDELD
                     LRLKVDGDTVADVAYDGEGCSISMASASAMTEAVIGRGLTDADDLAEAFRLMMHGEGV
                     KREEDLLDGVAFQGVAKFPVRVKCALLGWMALRDAIRTYAEGEAESRVTHE"
     gene            complement(4281..5516)
                     /locus_tag="VNU01_07485"
     CDS             complement(4281..5516)
                     /locus_tag="VNU01_07485"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWH32498.1"
                     /translation="MFDVGVIRKDFPVLDRTVHGRRLVYLDSAATAHKPVQVLEAMDR
                     FWERSNANVHRGVHALAEEATEQYEAARAKIARFVGADPRGTVFTKNATEAFNLLAYS
                     WARKKLGPGDIVLSTQMEHHANIVPWQLVQPDAGFELRYVPVTPDGVLDLDAFGEIVA
                     TGRVKLFTVTHMSNVVGTINPVRQMAEQVRAANREAIVVVDGAQSVPHLATDLLELGA
                     DFVAFSGHKMLGPTGIGVLATGKPELLESMDPFLGGGEMITNVTLEGTTFNEVPYKFE
                     AGTPPIGEAIGLGAAIDYLNGIGMDAVRAHEVELTRAAIAALEAVPGVTVHGPKDAHA
                     RGAAISFAIEGIHPHDIGTMMDREGVAVRAGHHCAKPLVRVLGSAATTRASFYLYNSP
                     EEIDALVAAIEATKRFFEH"
     gene            complement(5558..6298)
                     /gene="sufC"
                     /locus_tag="VNU01_07490"
     CDS             complement(5558..6298)
                     /gene="sufC"
                     /locus_tag="VNU01_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWH32499.1"
                     /translation="MAELEIKGLKVEVEGKEILKGLDLAVAKGETHALMGPNGSGKST
                     LAYAIMGHPSYAITGGQVLLKGTDITELSPDERAQLGLFLAMQYPTEIPGVSLTNFLR
                     TAINATRETDMPVRQFMTELREHMAALDMDEKFLSRNVNEGFSGGEKKRFEILQMAML
                     KPQVAVLDETDSGLDVDALKVVSEGVNRLRGPELGVLIITHYTRILRYITPDHVHVMF
                     EGTIVQSGGAELAAELEDKGYEHLKATA"
     gene            complement(6332..6688)
                     /locus_tag="VNU01_07495"
     CDS             complement(6332..6688)
                     /locus_tag="VNU01_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HWH32500.1"
                     /translation="MSWTRVAELADLKVGTPLRVELDEPLCVVRTGEDQVFAVHDVCS
                     HQEWSLAEGWVDDIDGRPHIECGLHGSSFDLADGSPDTLPAVKPIPTYAAKVEDGAVW
                     VDLAQQTNDAPVPQHY"
     gene            complement(6685..>7051)
                     /locus_tag="VNU01_07500"
     CDS             complement(6685..>7051)
                     /locus_tag="VNU01_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HWH32501.1"
                     /translation="HARATASDQTNRNLILTDGAKADSIPFLEIHNSDVVRCGHHSSV
                     GQVDELQLFYLESRGIPREEAARLLVFGFFAEVLERIDLPGVTEVVLGEIEREIRSGP
                     TTFMNQRRSSQGANKGEAA"
BASE COUNT         1017 a         2493 c         2408 g         1133 t
ORIGIN      
        1 tgctgctggc gcggccgttc cgcgcgttcg cgctgctgtt ccggccgctg atccgcctgc
       61 tgaacgccac ggcgaacgcg atcgtgcgcg cgctcggcgt ggagccacag gacgagctgg
      121 cgatgacgca ctcccccgcc gacctgctgc tgctgctgga ggagtccagc gagcagggca
      181 ccctggcgtc ggagcagcac gacctgctgt cgcgcgccct ggagctgggc ggcctggacg
      241 cccagtcggt gatggtgccg cgccgcgacg tggtggccgt gtcggcggag gcggacgccg
      301 gcgagatcga ggagctcgcg cgcgcgaccg gccgcagccg cctgccggtg tacgaggacg
      361 acctggaccg catccgcggt gtcctgcacg tcaaggacct gctggtgctg gacgccgacg
      421 agcgtgagcg cgtccgcgcc ggcgcgctgg cgcgcccggc gctcgtggtg ccggagtcgc
      481 ggccgctgga ggcgctgatg gtcgacatgc gccagcgccg ccagcacgtc gcgctggtca
      541 tcgacgagta cggcagcgtc tccggcctgg tcgcgctgga ggacctcatc gaggagctga
      601 tcggcgagtt cgaggacgag agcgaccggc gcgcccgcgt gttccgccgc cgcgccgacg
      661 gcgcctacct ggtgccggcg tccctgcggc ccgacgaggt cgaggagcgc ctgggcgtgg
      721 agctgccgga cggcgactgg gagacgatgg ccggcctggt gatcgcgcac ctggagcggc
      781 tgccggaggt cggcgacgtc gcggtggccg acggcgtgcg cctggaggtc acccgcgtcg
      841 acgcccaccg cctcatcgag ctggccctca ccccacgccc ggacccgacc ggcaacggtg
      901 gacacccgga gattggggcc tcctgaccgc aatattgcgg cccagcgtcc ccaaacctcc
      961 gttcaagcct ccaacacggc acggcaatcg cgcgccatgc gctcggcgta ttcgaccagc
     1021 ttcagcgcgt cggcacgggc agcagggtga ggccgtagtg cgcatcgtcc ttgcgcttca
     1081 ggatgcgatc gagatcccgt gccatccgtt tgccgtgagg ctccaccccc gccagcagtg
     1141 caaccgcctc ctggtggtcc gtgccccgag cgcgcacctt cagccgtgcg caacaggcag
     1201 cgtccgaagc agcgatgccg gccagcacag ccagggaggc agcgactccc ggtgccacgg
     1261 cgtcggtgtc atcctcgatg accagtctgg ccaccatcac gaacgcttcc gcctgtcgca
     1321 gacgtgaggc ggcgtccgct cggctgcatt gccgagtgcg catcacgatg ccgccccgtc
     1381 caaagccgat cgactgccca gcaccacgat gaggtcccgc ctccagcttt ccaggatcgg
     1441 ctcgtccacc cggcgcatgc cctcgagttg cgcgggcgtc accacaacga tgtgcccgtg
     1501 gttgcctgtc cagcgctcca catcggcagc gagcgcttcg gcgaaatcgc gagcatcgcc
     1561 tgagggagag accagcaata cgtcgatgtc gctcgtctcg tcaccatcgc gacgcgcagc
     1621 cgagccgaac agtccaagga gctggcagtc accatcccac tgcgccaccg cgccgcgcag
     1681 acgcgccgac aactcaccgt gaagaccagc gagcgcgacg acagcgggag cagcgacgtg
     1741 ctcacggttc aggacatacc cgcctggaac agcctcggcc agccccgcgt ccacgagccg
     1801 cagcagcaca cggcgcacac ccgcgagcga cccgcgtccc gcgacggccc gcacccgagt
     1861 cagagacagc ggctgtgtcg taccggcgag ggcggcaaga accggcccgt ccagtgacgg
     1921 gatcacgctc cgaatgggct gcgcaatgtc gatgacaagg ctccagggac gcttctagct
     1981 acagaatatc gtaacacttt tactattctg tagcacgcgc taccggagcg cgcacggtcc
     2041 gacccgagca gttgtccctg agcgggcacg atgagggggc cgcgtggaac tcgcggagcg
     2101 cggacgtaaa gggatcttgt tcgcgcgagg gagccgcgtg gcccccctag tacgtgggga
     2161 tgctcgggtc gacggtgcgc gcgtaggcgt tgatgccgcc gcgcatggac cgagccctca
     2221 ggccggcttc gcgcatcagc tcggcggccc gcagggagcg cgcgccggac ttgcacagca
     2281 cgacgtactc ggcgttgagc tccaggtcct gcagggagtc gggcagctcg gcgagcggga
     2341 tgagctcggc gcccgcgatg cggctgatct ggaactcgtg cggctcgcgc acgtccagca
     2401 gcttcacgcg ctcggccatc tccgcgtact cctgcggggt gacctccagc tcctcgggct
     2461 caccggcgcc ggtcgactcg tggtcgttgg ccggcacgcc gcagaactcc tcgtagtcga
     2521 tgagctcggt gaccgtcggg ttcttgccgc agacagggca ctcggggtcc ttgttgacct
     2581 tgatggtctg ccagcggttc tccagcgcgt cgtagatctg catgcggccg atgagcggct
     2641 cgccgatgcc cgtgatgagc ttgatgccct cggtggcctg gccggcgccg atggtcgcgc
     2701 agatcacgcc gaacacgccg ccctcggcgc agctcggcac catgccgggc ggcgggggct
     2761 cggggaacat gcagcggtag cacggcccgt gctccgccca gaacaccgac agctggccgt
     2821 cgaagcggaa gatcgagccg tacacgttcg gcttgcccag cagcacgcac gcgtcgttga
     2881 ccaggtagcg cgtcgggaag ttgtcggtcc cgtcgatcac caggtcgtag ggcgcgatga
     2941 tctccagcgc gttctcgctc agcagcgccg tctcgtggat gaccacctcg acgttggggt
     3001 tgatgtcctt gatggactcc atcgccgaga cgaccttgcg cttgccgatg tcggaggtcc
     3061 cgtggatcac ctggcgctgc aggttggaca ggtccaccac gtcgaagtcg accagcccga
     3121 tggtgccgac cccggccgcg gccaggtaca tcgccagcgg cgagcccagg ccgccggtgc
     3181 ccaccagcag gaccttggcg ttcttgaggc gcccctgccc gtccacgccc acgttcggca
     3241 tgatgaggtg ccgcgcgtag cgctggatct cctcgttggt cagcgacgcc ggctctgcga
     3301 tcaacggttc catgccaacc tcaactcatg gtgtcgtgga gcgcttcccc ccgctccggt
     3361 gaaaacgttg tctagacgga aaaccccatc gcgcgcagct cgtccttggc ctccggcgcg
     3421 atcatctccg tggaccacgg cggcgagaac acgaagttca cgtccacgtc ctccacgccg
     3481 ggcaggcgcg tcaggatcat cgtgcactgc tggtggatga gctcggtcag cgggcagccc
     3541 agggacgtca gcgtcatcgt gagcttgacc ttgcccggcg gctccttctc cacgccgtag
     3601 accaggccga ggtcgacgat gttgatgccg atctcggggt ccagcaccgc cttcagcgcg
     3661 gtgcgcacgg cctcgtccgt cggcaggccg tcgtcgtcca gcggcacgtc ggcgaaggcc
     3721 tgcacctgca cctgcgcctg cgcctgggcg gcgtcgtact ccggcgtgcc cggctccggt
     3781 tcgatcggcc agccctgcag cgccgcgtca ctcatgggtc acccggcttt ccgcctcgcc
     3841 ctcggcgtag gtgcggatcg cgtcgcgcaa cgccatccac cccagcagcg cgcacttcac
     3901 gcgcacgggg aacttcgcca cgccctggaa cgcgaccccg tccaggaggt cctcctcccg
     3961 cttgacgccc tcgccgtgca tcatcagccg gaaggcctcc gcgaggtcgt cggcgtcggt
     4021 gaggccgcgc ccgatgaccg cctcggtcat cgccgaggcc gacgccatgg agatggagca
     4081 gccctcgccg tcgtaggcga cgtcggcgac ggtgtcgccg tccaccttca ggcgcaggtc
     4141 cagctcgtcg ccgcacagcg ggttggagtg gtggacgtgc acctgggcgc cctcgagctc
     4201 cgcggcccgg tgccgcgggc tgcggtagtg gtcgaggatg atctcgtggt agaggtcgtc
     4261 gagcgacaca gtgatctctc ctagtgctcg aagaagcgct tggtcgcctc gatcgccgcg
     4321 acgagcgcgt cgatctcctc cggcgagttg taaaggtaga acgacgcgcg ggtggtcgcg
     4381 gcactgccga gcacccgcac cagcggcttg gcgcagtggt ggccggcgcg cacggccacg
     4441 ccctcgcggt ccatcatcgt gccgatgtcg tgcgggtgga tgccctcgat cgcgaagctg
     4501 atcgccgcgc cgcgcgcgtg cgcgtccttg ggcccgtgca ccgtcacccc gggcaccgcc
     4561 tccagcgcgg cgatggcggc ccgggtgagc tcgacctcgt gggcccgcac cgcgtccatg
     4621 ccgatgccgt tcaagtagtc gatcgcggcg cccaggccga tcgcctcgcc gatcggcggc
     4681 gtgcccgcct cgaacttgta cggcacctcg ttgaacgtgg tgccctccag cgtgacgttg
     4741 gtgatcatct cgccgccgcc caggaacggg tccatggact ccaggagctc cggcttgccg
     4801 gtggccagca cgccgatgcc cgtggggccg agcatcttgt ggccgctgaa cgcgacgaag
     4861 tccgcgccca gctccagcag gtcggtggcc aggtgcggca cgctctgcgc gccgtcgacc
     4921 accacgatcg cctcgcgatt ggccgcgcgc acctgctcgg ccatctggcg caccgggttg
     4981 atggtgccga cgacgttgga catgtgcgtc acggtgaaca gcttcacgcg tccggtcgcc
     5041 acgatctcac cgaacgcgtc cagatcgagc acgccgtcgg gggtcaccgg cacgtagcgc
     5101 agctcgaagc cggcgtcggg ctgcacgagc tgccacggca cgatgttggc gtggtgctcc
     5161 atctgcgtcg acagcacgat gtcgcccggg ccgagctttt tgcgcgccca gctgtaggcc
     5221 agcaggttga acgcctccgt ggcgttcttg gtgaacacgg tgccgcgcgg gtcggcgccc
     5281 acgaagcgcg cgatcttggc gcgggcggcc tcgtactgct cggtcgcctc ctccgccagc
     5341 gcgtgcacgc cgcggtggac gttggcgttg gaccgctccc agaagcggtc catggcctcc
     5401 aggacctgca ccggcttgtg ggccgtcgcg gccgagtcca ggtacaccag ccggcgcccg
     5461 tggaccgtgc ggtccaggac cgggaagtcc ttgcggatga ctcctacgtc gaacaagtgc
     5521 tctaccgcct tcggctactt gagagcgggt gtgcgcgtca cgccgttgcc ttcaggtgct
     5581 cgtagccctt gtcctccagc tcggcggcga gctccgcgcc accggactgg acgatggtgc
     5641 cctcgaacat gacgtggacg tggtcggggg tgatgtagcg caggatgcgc gtgtagtggg
     5701 tgatgatcag cacgcccagc tccgggccgc gcaggcggtt cacgccctcc gacaccacct
     5761 tgagcgcgtc gacgtccagg ccggagtcgg tctcgtccag cacggcgacc tgcggcttca
     5821 gcatcgccat ctgcaggatc tcgaagcgct tcttctcacc gccggagaag ccctcgttga
     5881 cgttgcgcga caggaacttc tcgtccatgt ccagcgcggc catgtgctcg cgcagctcgg
     5941 tcatgaactg gcgcaccggc atgtcggtct cgcgcgtggc gttgatcgcc gtgcgcagga
     6001 agttcgtcag cgacacgccc gggatctccg tcgggtactg catcgccagg aacaggccca
     6061 gctgcgcgcg ctcgtccggg gacagctcgg tgatgtcggt gcccttgagc agcacctgcc
     6121 cgccggtgat cgcgtaggac ggatggccca tgatcgcgta ggccagcgtg gacttgccgg
     6181 agccgttggg gcccatcagc gcgtgcgtct cgcccttggc cacggccagg tccaggccct
     6241 tgaggatctc cttgccctcg acctcgacct tgaggccctt gatttctagc tctgccatgt
     6301 ctgctcgcac ttccctcgtc gtcgaagtcg tctagtaatg ctgcggcacg ggcgcgtcgt
     6361 tggtctgctg cgccaggtcc acccacacgg cgccgtcctc caccttggcg gcgtaggtcg
     6421 ggatgggctt gaccgccggc agggtgtcgg ggctgccgtc ggcgaggtcg aaggacgacc
     6481 cgtgcaggcc gcactcgatg tgcggacggc cgtcgatgtc gtcgacccag ccctcggcca
     6541 gggaccactc ctgatgggag cagacgtcgt gcaccgcgaa gacctggtcc tcgccggtgc
     6601 gcaccacgca cagcggctcg tccagctcca cgcgcagcgg cgtgccgacc ttgaggtcgg
     6661 cgagctccgc caccctggtc cagctcatgc cgcctcaccc ttgttggcgc cctgggacga
     6721 gcggcgctgg ttcatgaacg tggtgggccc gctgcggatc tcgcgctcaa tctcgcccag
     6781 caccacctcg gtgacgcccg gcaggtcgat gcgctccagc acctcggcga agaagccgaa
     6841 caccagcagg cgcgcggcct cctcgcgggg gatgccgcgg gactccaggt agaacagctg
     6901 cagctcgtcg acctggccca cggagctgtg gtggccgcag cggaccacgt cgctgttgtg
     6961 gatctccagg aacgggatgg agtcggcctt cgccccgtcc gtgaggatga ggttgcggtt
     7021 ggtctggtcc gacgccgtgg cgcgcgcgtg c
//
LOCUS       DATMKC010000258         3822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_36282, whole genome shotgun sequence.
ACCESSION   DATMKC010000258 DATMKC010000000
VERSION     DATMKC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36282"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..1999
                     /locus_tag="VNU01_07505"
     CDS             428..1999
                     /locus_tag="VNU01_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32502.1"
                     /translation="MLVSALVGALSLGAAWAGAVLTPARTAGPDVGELPEQAGGPFRA
                     SPSPSPTPDPLPGLQGGEVYAGAAKVNIAPRPGPDDVWIRDQAACETLTAPDRWVEHV
                     QDLHTLWPENPGCIYTGGYGIGPANAVSEFDTEYGLWARSVALGDGTETVVLTVVDGV
                     YWQGRYGKMCDGCGAFDLAEQLSAELAAHGVKRSGLFFAATHSHTAPDFIGGWGGVPK
                     WYMEQVAEAMRDSVRQAVASLRPATVEAGEILARDHNKDRRTTYYSAEEAGMSWLRAV
                     GRDGEVVATLGAYAAHPTTSDEEIGVAHADWPGLYTKRLEDRFGGVGLHFMTGLGQMT
                     ARGGRNVGVTLADLVPEVGRGQAVGSPDAKPAVRSAAAMWDQPITNGVLAALGGAGLF
                     DRPFGGPAQVSAGRSAQRPCVSAAPISVNTGVNAARVGNLVFTGAPGETFANLSNSIK
                     ERRTGQVTFPLALVNDGLGYIMQSIETFDEARQGLGFAGSDATEYEDAYSIDRCFGDK
                     VLDESIRLVESVWGS"
     gene            2010..2903
                     /locus_tag="VNU01_07510"
     CDS             2010..2903
                     /locus_tag="VNU01_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWH32503.1"
                     /translation="MQHLLTRRGLLKAAGVGALAAAAPMGALATPLFMPSDTTGTGAS
                     RQSRLERGNRTRLAHGDLHNHSHLSDGTGGPELVFDSIRRAGLDFAALTDHSTFSWGA
                     VGAVDPCPHTQDYEHGERQECQSVAGLDEAAWAYTRELADGANHDHHFAAIRGFEWSS
                     PFLGHVNVWFSERWIDPLHTAGIGPEGIGQHLHQNLGPLGEAAQPPADAALRANPVRT
                     GMAPFYRWLALDPSTPASAAGWTAWPASTTRGESRAASPTSPTTPRRRGAWSPSRCST
                     AARTTCSRASPTASRPRWWSA"
     gene            2828..3439
                     /locus_tag="VNU01_07515"
     CDS             2828..3439
                     /locus_tag="VNU01_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32504.1"
                     /translation="MFNRGEDYLFEGFADGQPSPLVECLNAGWRVGLIGVTDEHGTNW
                     GFNPGKGRAGLWVPSLTRGGVREALGNRAMFATREDGLRLDATANRRRMGGDVGPSQG
                     PDRSLVRFEVDVDRGPQWWGTPVEVQVLRPGTAFPEVVHVKSTTLRRDDEPVISFQVP
                     LSKDDGDWVLLRVADPSQPNRQPGPAGHACNNLAFAYSSPWWP"
     gene            3544..>3822
                     /locus_tag="VNU01_07520"
     CDS             3544..>3822
                     /locus_tag="VNU01_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32505.1"
                     /translation="MAMAMMQPPGETEFATRQDVQRVDEKVDRVGEQVRRLETGFERM
                     EEKLDAVLTVMREHMASKADVERMGRIMTIWVVGAITALIGIMLTTMWN"
BASE COUNT          544 a         1389 c         1389 g          500 t
ORIGIN      
        1 ggacgggcca gaagggccgg ctccggcgtc ccgaccacgc cggagccggc cccctcacca
       61 ccaagagttc cgccccggtc ccttccgagg gaccacccca ccgaaacagc accaccccac
      121 cgagcatcac aaaaccccat cgcagcatct ccccggtccg gcaggttcgt ccggccgaac
      181 gggggagact ggaggaccgg gacgcccgcc agccccggtc ctccggcttt tcctgccgct
      241 tctcccccgc ttcttccccg agttggaaga tccggcccgt atatgggcct gatgttccaa
      301 ctcgggcgat gtgggcggcc gacattgtcg gattcccggc agggggcgac ctgccgcgtg
      361 gcgaatacat ccgcctatgc gcgaatctca ccgtccctcc gccctcgtcc accgcacggg
      421 caggatcgtg ctcgtctccg cgctggtggg cgcgctgtcc ctgggggccg cctgggccgg
      481 cgccgtgctc acgcccgccc gcaccgccgg gcccgacgtc ggcgagctgc ccgagcaggc
      541 gggcgggccg ttccgcgcca gcccctcccc cagcccgacg ccggacccgc tgccgggcct
      601 gcagggcggc gaggtctacg cgggcgcggc caaggtgaac atcgcgccgc ggccgggacc
      661 ggacgacgtc tggatccgcg accaggcggc gtgcgagacc ctgaccgcac ccgacaggtg
      721 ggtcgagcac gtccaggacc tgcacacgct gtggccggag aacccgggct gcatctacac
      781 cggcggctac ggcatcgggc cggcgaacgc cgtgtcggag ttcgacaccg aatacggcct
      841 ctgggcgcgg tccgtcgccc ttggcgacgg caccgagacc gtggtgctga cggtggtcga
      901 cggcgtctac tggcagggcc gctacggcaa gatgtgcgac ggctgcggcg cgttcgacct
      961 ggccgagcag ctgtccgcgg agctcgccgc gcacggtgtg aagcggtccg gcctgttctt
     1021 cgccgccacc cacagccaca ccgcgccgga cttcatcggc ggctggggcg gcgtgcccaa
     1081 gtggtacatg gagcaggtgg ccgaggccat gcgcgacagc gtccgccagg ccgtcgcgtc
     1141 gctgcgcccc gccacggtcg aggcgggcga gatcctggcg cgcgaccaca acaaggaccg
     1201 ccgcaccacg tactactccg ccgaggaggc ggggatgtcg tggctgcgcg cggtcgggcg
     1261 cgacggcgag gtggtcgcga ccctgggcgc ctacgccgcc caccccacca ccagcgacga
     1321 ggagatcggc gtcgcgcacg cggactggcc gggcctttac accaagcggc tcgaggaccg
     1381 cttcggcggc gtcggcctgc acttcatgac cggtctgggg cagatgacgg ccaggggcgg
     1441 gcgcaacgtc ggcgtgacgc tggcggacct ggtgcccgag gtgggccgcg ggcaggccgt
     1501 cggctcgccg gacgccaagc cggcggtgcg cagcgccgcg gcgatgtggg accagccgat
     1561 caccaacggc gtgctggcgg cgctgggcgg cgcgggcctg ttcgaccgtc ccttcggcgg
     1621 cccggcgcag gtgtccgcgg gacgctccgc gcagcggccc tgcgtgtccg ccgcgcccat
     1681 ctcggtcaac accggcgtca acgccgcgcg cgtcggcaac ctggtgttca cgggcgcgcc
     1741 cggggagacg ttcgcgaacc tgtccaactc catcaaggag cgccgcaccg ggcaggtcac
     1801 gttcccgctg gccctggtca acgacgggct cggctacatc atgcagagca tcgagacgtt
     1861 cgacgaggcg cggcaggggc tgggcttcgc cggcagcgac gccaccgagt acgaggacgc
     1921 ctactccatc gaccgctgct tcggcgacaa ggtgctggac gagagcatcc ggctcgtcga
     1981 gtcggtctgg ggcagctgag ggggatccga tgcagcacct gctgacccgg cgcggcctgc
     2041 tcaaggccgc cggcgtgggc gcgctggcgg ccgcggcgcc gatgggcgcc ctggccacgc
     2101 cgctgttcat gccgagcgac accaccggca cgggcgcgtc gcgccagtcg cggctggagc
     2161 gcggcaaccg cacgcgcctg gcccacggcg acctgcacaa ccacagccac ctgtcggacg
     2221 gcacgggcgg gccggagctg gtgttcgact ccatccgccg ggcgggcctg gacttcgccg
     2281 cgctgacgga ccactcgacg ttcagctggg gcgccgtcgg cgcggtggac ccgtgcccgc
     2341 acacgcagga ctacgagcac ggcgagcgcc aggagtgcca gtccgtggcc ggcctggacg
     2401 aggccgcctg ggcgtacacg cgcgagctcg ccgacggcgc gaaccacgac caccacttcg
     2461 ccgcgatccg cggcttcgag tggtcttcgc cgtttttggg ccacgtgaac gtgtggttct
     2521 ccgagcgctg gatcgacccg ctgcacaccg ccggcatcgg cccggagggc atcggccagc
     2581 acctgcacca gaacctcggg ccgctgggcg aggcggcaca gccgcccgcc gacgccgcgc
     2641 tgcgcgcgaa cccggtgcgc accggcatgg cgccgttcta ccgctggctg gcgctggacc
     2701 cgtcgacgcc cgcatcggcg gcgggctgga cggcctggcc ggcttcaacc acccggggcg
     2761 agagcagggc cgcttctcct acttctccta cgacgccgcg gcggcgcggc gcctggtctc
     2821 catcgaggtg ttcaaccgcg gcgaggacta cctgttcgag ggcttcgccg acggccagcc
     2881 gtccccgctg gtggagtgcc tgaacgccgg ctggcgcgtg ggcctgatcg gcgtgaccga
     2941 cgagcacggc accaactggg gtttcaaccc cggcaagggc cgcgctggcc tgtgggtgcc
     3001 gtcgctgacg cgcggcggcg tgcgcgaggc gctgggcaac cgggcgatgt tcgccacgcg
     3061 ggaggacggg ctgcgcctgg acgccaccgc gaaccgccgg cgcatgggcg gcgacgtggg
     3121 accgtcccag ggcccggacc gctcgctggt gcgcttcgag gtggacgtcg accgcggccc
     3181 gcagtggtgg ggcacgcccg tcgaggtgca ggtcctgcgg cccgggacgg cgttcccgga
     3241 ggtggtgcac gtgaaatcca ccacgctgcg ccgcgacgac gagccggtga tcagcttcca
     3301 ggtgccgctg tcgaaggacg acggggactg ggtgctgctg cgcgtcgcgg acccgtccca
     3361 gccgaaccgc cagccgggcc cggccgggca cgcctgcaac aacctcgcgt tcgcctattc
     3421 ctcaccctgg tggccgtaac cggtccacag ggtttcccac agattctccg gacccccttc
     3481 cgcgcgcccg tttcgcgggg agactggccc cagtttcgcg ggcgcgggaa ggagacgagg
     3541 aggatggcca tggcgatgat gcaaccgccg ggcgagacgg agttcgccac aaggcaggac
     3601 gtgcaacgcg tcgacgagaa ggtcgatcgg gtgggagagc aggtgcggcg actcgagacc
     3661 ggattcgaac gcatggagga aaaactggat gcggtgctca cggtcatgcg tgagcacatg
     3721 gcgtccaagg ccgatgtcga gcgcatgggc cgcatcatga ccatctgggt ggtcggcgcc
     3781 atcaccgcgc tcatcgggat catgctcacg accatgtgga ac
//
LOCUS       DATMKC010000259         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_335972, whole genome shotgun sequence.
ACCESSION   DATMKC010000259 DATMKC010000000
VERSION     DATMKC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335972"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..771
                     /locus_tag="VNU01_07525"
     CDS             148..771
                     /locus_tag="VNU01_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWH32506.1"
                     /translation="MLVRATAILLTLAALAGGGTAAYTVRPGDTLSGIAARLGVSLRA
                     LADANGITDPDRVLAGRSLRVPAAAGAPAAPATRADVAAQLEGTARRYGWSAAFVKAL
                     AWQESGWNQGVRSPAGAVGVMQVMPGTGRFVSRHLVGRSLDLAHTADNIEAGVAFLDY
                     LYRLTGRDPELTLAGYYQGLASVRRHGMHDGTRRYVANVLALRERFR"
     gene            complement(785..1366)
                     /locus_tag="VNU01_07530"
     CDS             complement(785..1366)
                     /locus_tag="VNU01_07530"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HWH32507.1"
                     /translation="MRRRGPAPGPLGITPAEGALLSLLSLLLGVGVVAPPAPTEDAGR
                     PLAAAAPAVPAIATAPAEPATTATRVVPPRPAPAAASRARSAPARAAVRPVAGGLELW
                     LLLADCESGFRDRHGRVIRGSARWHLDSGNGYYGAFQFAPESWAWTGGRGLPHEASPR
                     EQLHRAAVLQRRIGWARAWPVCSRMLGLAPPAA"
     gene            complement(1463..1651)
                     /locus_tag="VNU01_07535"
     CDS             complement(1463..1651)
                     /locus_tag="VNU01_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32508.1"
                     /translation="MQELHCSTCELPIVERAGDPGYWTDSDGWFVSEFPSLHAHRPAD
                     DIALLVELRDEDSLSAHR"
     gene            complement(1757..1930)
                     /locus_tag="VNU01_07540"
     CDS             complement(1757..1930)
                     /locus_tag="VNU01_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32509.1"
                     /translation="MDQLMQKYAVGGFCSGLLAGGLMVFMLNVLGGGDNLGMPFAFAV
                     AGAAGAYTQLKNA"
BASE COUNT          268 a          774 c          739 g          231 t
ORIGIN      
        1 gtaaaccggt tgaccagggg cactggccgc cgccaggacg gccgatcgcc accgtcgcca
       61 catctttaca ccacaggtgt tcacaacagc cacaacagcg cacaggatga acagcggccc
      121 gtttccgacc gctgcacgga ggtggacatg ctcgtccgcg cgaccgccat cctgctcacc
      181 ctggccgccc tggccggcgg cggcaccgcc gcctacaccg tgcgccccgg cgacacgctc
      241 agcggcatcg ccgccaggct cggcgtgtcg ctgcgcgcgc tggcggacgc caacggcatc
      301 accgatcccg accgcgtcct ggcgggccgc agcctgcgcg tgcccgcagc ggccggcgcg
      361 cccgccgcac cggccacgcg cgcggacgtg gccgcccagc tcgagggcac cgcgcggcgg
      421 tacggctgga gcgcggcgtt cgtgaaggcg ctcgcctggc aggagtccgg ctggaaccag
      481 ggcgtgcgct cccccgccgg ggcggtgggc gtcatgcagg tcatgccggg caccgggcgc
      541 ttcgtgtcgc gccacctggt cgggcgctcg ctggacctgg cccacaccgc cgacaacatc
      601 gaggcgggcg tggcgttcct ggactacctg taccggctca ccgggcggga tccggagctg
      661 accctggccg gctactacca ggggctggcc tccgtgcggc gccacggcat gcacgacggc
      721 acccgccgct acgtcgccaa cgtcctggcg ctgcgcgaac ggttccgctg agcgcgccgc
      781 ccgctcaggc cgccgggggc gccagcccca gcatgcgcga gcacaccggc caggcgcgcg
      841 cccagccgat gcgccgctgc agcacggcgg cgcggtgcag ctgctcgcgc ggggacgcct
      901 cgtgcggcag cccgcgcccg cccgtccagg cccaggactc gggcgcgaac tggaacgcgc
      961 cgtagtagcc gttgccggag tccaggtgcc agcgcgccga accgcggatc acgcgcccgt
     1021 ggcggtcgcg gaacccggac tcgcagtcgg ccagcagcag ccacagctcc aggccgccgg
     1081 ccacggggcg caccgccgca cgggcgggcg cggaacgggc gcgcgaggcg gccgcgggcg
     1141 cgggccgggg cggcaccacg cgggtcgccg tcgtcgccgg ctccgccggc gcggtggcga
     1201 tcgcgggcac ggccggcgcg gcggcggcga gcggccggcc ggcgtcctcg gtcggggccg
     1261 gcggcgccac gacgccgacg cccaggagca ggctcagcag ggacagcagc gcaccctccg
     1321 cgggcgtgat gccgaggggt ccgggagcag ggccgcgtcg ccgcacgggc cctggcgtgc
     1381 ccaggcgcgc cggcgttcat gcgcgcgggc cgtcacgcgg acggcccgcg gtggggcgcc
     1441 gaggtgagcc gggggaggca gctcagcggt gcgcgctcag cgagtcctcg tcgcgcagtt
     1501 ccaccaggag cgcgatgtcg tcagccggac ggtgggcgtg cagcgacggg aactcgctca
     1561 cgaaccagcc gtcggagtcg gtccagtagc cgggatcccc ggcgcgttcg acgatgggca
     1621 gttcacaggt gctgcagtgg agttcctgca cggtgcctcc ttccgcggcg accccccgcc
     1681 gggaagcagt ctgctcctac gacacctgtc ggtggaaccc ttgattcggg gaacttttcc
     1741 tccccttcag gacgagtcag gcgttcttca gctgcgtgta ggcgcccgcg gcgcccgcca
     1801 cggcgaacgc gaacggcatg cccaggttgt cgccgccgcc cagcacgttg agcatgaaca
     1861 ccatcagccc gcccgcgagc agcccggagc agaacccccc gaccgcgtac ttctgcatca
     1921 actggtccac gaacccgcct cccgtcgcgc tctgcgtcac cgcaggataa cgcgtgccgg
     1981 gccccgcggg ttctacgcga gcagttggcg at
//
LOCUS       DATMKC010000260         6206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_11732, whole genome shotgun sequence.
ACCESSION   DATMKC010000260 DATMKC010000000
VERSION     DATMKC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11732"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VNU01_07545"
     CDS             <1..1136
                     /locus_tag="VNU01_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWH32510.1"
                     /translation="SAGRRRRGRPALAAFAPGRGSRASGIHERVSGVPATTVVAGTPE
                     TRSWMPDARLPRPGAKAAKAGRPRRRLPALLAAVLAVALAATGVWAFVLAPPVEVPQL
                     TRMTLDEARAALADRGVELAQADAIHSLDVAEGTIAAQDPAAGANVRKGGTVTVALSL
                     GPRVVTMPRVLGLTRAEAEAELDGHHFALRVDEGWHDSAPAGTVAAQIPEPGADIAEG
                     ADVVLYVSKGVEQVTVPDVGDLDRDAAVAALKDAKLTPQVVEVYSDDVPEAGAVIGQS
                     VEAGSVVDKGTVVEVTVSKGPLTVKVPNVEGKAIDEGRRILEGLGLRVTVIEEPRPQV
                     GPFKRGQYGRVEAQDPQPGKKVKRGDTVRLYTFSKAADERDDD"
     gene            1145..2080
                     /locus_tag="VNU01_07550"
     CDS             1145..2080
                     /locus_tag="VNU01_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWH32511.1"
                     /translation="MTAVRHLRRYAWEAALIGVTLVWGSTFVVVKQGVDSVPVYSFNA
                     IRFALAAVVLAACCAPGLRRLGRDGLRHGAVLGVFLFAGYAFQTVGLRHTDASRAGFI
                     TGMFVVFTPLLAALVLRRRPSASAWAGVALATLGLALLSLRGSLLPAFGDVLVLGCAL
                     AFAAHIVGLGAWSNRHAALPLTAVQLGVAAALHTAVALVAEVPRASYHWDGRVVVALG
                     LTAVLASAAGFWVQTAAQRVIPPTRTAIILTMEPVFAGLFGYVLLGERLALRGWAGAL
                     LILAGMLTAELGAAREPDLDEEQRDAASRQPAGMP"
     gene            complement(2059..2907)
                     /locus_tag="VNU01_07555"
     CDS             complement(2059..2907)
                     /locus_tag="VNU01_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HWH32512.1"
                     /translation="MAPACPRCDVAGQEGSFCTSCGYDLRPPGPAVVSPRHRAAAEAP
                     PEIVACRACGARNAKSRQVCGRCRAPLSGDAADAVPPPPRAQGFGPAVRVEPPVPKPS
                     RFLLLVLGLAALVLAGATVTLLVARGIGPFTPEAPEAGPLAALAVERVRASSAQAGEP
                     PQRLLDGDAGTAWAEDGAGPGVGQWVELTLARRARVHRLAIWNGDQRGDRFLARNRVR
                     ELRIDTAGERFVVELLDTQGQQVVELPRTVRADRLRLTILSVHGAAPDANTALSELEV
                     YGIPAG"
     gene            complement(2916..3131)
                     /locus_tag="VNU01_07560"
     CDS             complement(2916..3131)
                     /locus_tag="VNU01_07560"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HWH32513.1"
                     /translation="MADNTYKVTEIVGTSTDSVQQAIRNGVERAARTLRNLDWFEVTE
                     IRGHIEDGHVAHFQVGMKVGFRLDDES"
     gene            complement(3149..3730)
                     /locus_tag="VNU01_07565"
     CDS             complement(3149..3730)
                     /locus_tag="VNU01_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWH32514.1"
                     /translation="MSANADRLPPGQYEARGWPVLHFGAVPRVDLATWRFRIFGAVER
                     ERELTLDEFKALPRAGVRADFHCVTKFSVFDNDWEGVAFRTVYELVTPDPEVSHAMVW
                     SHDGYSANLPLEALLDDDVLFAWARNGEPLTPEHGAPLRLVVPKRYAWKSAKWVKGLE
                     LMTRDRRGFWEERGYHNQADPWNEERYSYQETA"
     gene            complement(3741..4778)
                     /locus_tag="VNU01_07570"
     CDS             complement(3741..4778)
                     /locus_tag="VNU01_07570"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HWH32515.1"
                     /translation="MIDLRSDTVTKPTPQMRRAMAEAEVGDEVYREDPTVRRLEEQAA
                     ALLGREAALYCPTGVMCNQIWLRLLARPGTEVVVEGDSHLVNYEAGAGAILAGVQFRT
                     LSAADPQRRGILAADQVQAALKPDVFPLTPTTLVWVEQTHNRGGGTCYTLDELRGLRK
                     LTAEAGVALYMDGARVFNAAAATATPAAEFAAEVDGLMFCLSKGLGAPVGSLMVGDAA
                     HIEQARLWRQRYGGGMRQAGVIAAAGLVALRDVDRLHEDHENAQVLARAAADVHPDGI
                     DPARVQTNIVSIEGVDAPAVIAALAERGVLAGPMDPRTVRLVTHRDVSRSDCERAADA
                     LRATLPGPGLG"
     gene            4901..5497
                     /locus_tag="VNU01_07575"
     CDS             4901..5497
                     /locus_tag="VNU01_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32516.1"
                     /translation="MLTRDGRGAPVHAELVEDVTLVRDLTPARWVEESMVPWPPREAG
                     LAAPVGSVVPACFAAYLRVHHRGGADGLAPDQAGALLEVLLRHTATGDAGWICVWEGW
                     GGSPPEFDAAPKVSTPARAHVLLRGSLRLVETRLMRDPFGRHSFDPSLFWPDDRAWCV
                     ASDTDLAWSYVGGSADCIAALAAVFPDAVTVRPEDPVV"
     gene            5601..>6206
                     /locus_tag="VNU01_07580"
     CDS             5601..>6206
                     /locus_tag="VNU01_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH32517.1"
                     /translation="MCSSAGFEIDAALATADLHGLLDVAERVLRRAKALPLGGAVDDV
                     ALTQAVQRLEALSRVAESHKCAVVGEIEQRHAARGQSAGSTEDLLTRVLHLTPGEARA
                     RTELAAGLKLLPDTAAAFADGRMGVSQAAVAVRKAAEVAGRDDAEVLLATIDRTASTV
                     GQTVNRTRLAREIDTAVAKTGVDVLAERERRAFQDRKLQWSV"
BASE COUNT          735 a         2282 c         2358 g          831 t
ORIGIN      
        1 gcagcgccgg caggcgccgt cgcggccggc cggccttggc ggccttcgcg cccggccggg
       61 gcagccgcgc gtccggcatc cacgagcgcg tctccggcgt acccgccacc accgtggtgg
      121 cgggtacgcc ggagacgcgc tcgtggatgc cggacgcgcg gctgccccgg ccgggcgcga
      181 aggccgccaa ggccggccgg ccgcgacggc gcctgccggc gctgctcgcc gccgtgctgg
      241 ccgtggcgct ggcggccacc ggcgtgtggg ccttcgtgct cgcgccgccg gtcgaggtgc
      301 cccagctcac gcgcatgacg cttgacgagg cccgcgcggc gctcgccgac cgcggcgtgg
      361 agctggcgca agccgacgcc atccattccc tcgacgtcgc cgagggcacc atcgccgcgc
      421 aggacccggc ggccggggcc aacgtgcgca agggcggcac ggtgaccgtc gcgctgtcgc
      481 tgggaccgcg cgtcgtcacg atgccgcgcg tgctgggcct gacccgcgcg gaggccgagg
      541 ccgagctcga cgggcaccac ttcgccctcc gtgtcgacga gggctggcac gactccgcgc
      601 ccgcgggcac cgtcgccgcg cagatcccgg agcccggcgc cgacatcgcc gagggcgccg
      661 acgtggtgct gtacgtgtcc aagggcgtgg agcaggtcac cgtgcccgac gtcggcgacc
      721 tggaccgtga cgcggccgtg gccgcgctga aggacgccaa gctgaccccg caggtcgtcg
      781 aggtgtacag cgacgacgtg ccggaggcgg gcgccgtgat cggccagtcc gtggaggccg
      841 gcagcgtggt cgacaagggc acggtggtcg aggtcaccgt ctccaagggc ccgctcaccg
      901 tgaaggtgcc caacgtggag ggcaaggcca tcgacgaggg ccggcgcatc ctggaaggcc
      961 tcggccttcg ggtcaccgtg atcgaggagc cgcgccccca ggtcggcccg ttcaagcgcg
     1021 gccagtacgg gcgcgtggag gcgcaggacc cccagcccgg caagaaggtc aagcggggcg
     1081 acaccgtgcg cctgtacacg ttcagcaagg ccgcggacga acgcgacgac gactaggaca
     1141 gcgcgtgacc gcggtgaggc acctgcgccg ctacgcctgg gaggcggcgc tcatcggcgt
     1201 caccctcgtg tggggatcga ccttcgtggt cgtgaagcag ggcgtggaca gcgtgcccgt
     1261 gtactccttc aacgccatcc gcttcgcgct ggccgcggtg gtgctcgccg cgtgctgcgc
     1321 gccgggcctg cgccgcctcg ggcgcgacgg cctgcgccac ggggcggtgc tcggggtgtt
     1381 cctgttcgcc ggctacgcgt tccagaccgt gggcctgcgc cacaccgacg cgagccgcgc
     1441 cggcttcatc accggcatgt tcgtggtgtt cacgccgctg ctggccgccc tggtgctgcg
     1501 ccggcgcccc tcggcgtccg cgtgggccgg cgtcgcgctg gcgacgctcg gcctggccct
     1561 gctgagcctg aggggctcgc tgctgccggc cttcggcgac gtgctggtgc tcgggtgcgc
     1621 gctggcgttc gccgcgcaca tcgtgggact gggcgcgtgg tcgaaccggc acgcggccct
     1681 gccgctgacc gccgtgcagc tcggcgtggc ggccgcgctg cacacggcgg tggcgctggt
     1741 cgcggaggtg ccgcgggcga gctaccactg ggacgggcgg gtggtggtcg cgctggggct
     1801 caccgccgtg ctcgccagcg ccgcgggctt ctgggtgcag accgccgccc agcgcgtcat
     1861 cccgcccacc cgcaccgcca tcatcctcac catggagccc gtgttcgccg ggctgttcgg
     1921 ctacgtgctg ctgggcgagc ggctggcgct gcgcggctgg gcgggcgcgc tgctgatcct
     1981 ggcaggcatg ctgaccgccg agctcggcgc cgcccgggag cccgacctgg acgaggagca
     2041 gcgggacgcc gcgagccgtc agccggccgg gatgccgtag acctccagct ccgacagcgc
     2101 cgtgttcgcg tccggcgcgg cgccgtgcac gctcaggatc gtcaggcgca gccggtcggc
     2161 ccgcaccgtg cgcggcagct ccaccacctg ctggccctgc gtgtccagca gctccaccac
     2221 gaagcgctcc cccgcggtgt cgatgcgcag ctcccgcacg cggttgcgcg ccaggaagcg
     2281 gtcgccgcgc tggtcgccgt tccagatcgc caggcggtgc acgcgcgcgc ggcgggccag
     2341 cgtgagctcc acccactggc cgacgccagg cccggcgcca tcctccgccc aggcggtgcc
     2401 ggcatccccg tccagcaggc gctgcggcgg ctccccggcc tgcgccgacg acgcgcgcac
     2461 gcgctccacc gccagcgcgg ccagcggccc ggcctcgggc gcctcaggcg tgaacgggcc
     2521 gatgccgcgc gccaccagga gcgtcacggt ggcgccggcc agcaccaggg cggccaggcc
     2581 cagcaccagc agcaggaagc gcgacggctt cggcaccggg ggctcgaccc gcacggcggg
     2641 gccgaagccc tgcgcgcgcg gcggtggcgg gacggcgtcg gcggcgtccc cgctcagcgg
     2701 cgcgcggcag cggccgcaga cctgccggct cttggcgttg cgcgcgccgc aggcccggca
     2761 ggccacgatc tccggcggcg cctcggccgc cgcgcggtgg cgggggctga cgaccgcggg
     2821 ccccggcggc cgcaggtcgt agccgcagga ggtgcagaaa ctgccctcct gtcccgcgac
     2881 gtcgcagcgc gggcacgccg gggccaccgc gggccctagg actcgtcgtc gaggcggaag
     2941 cccaccttca tgcccacctg gaagtgcgcg acgtgaccgt cctcgatgtg gccgcggatc
     3001 tcggtcacct cgaaccagtc caggttgcgc agcgtccgcg cggcgcgttc gacgccgttg
     3061 cggatcgcct gctggacgct gtcggtcgac gtcccgacga tctccgtcac cttgtaggtg
     3121 ttgtcggcca tgagcggacc tttcctgttc aggcggtctc ctggtaggag taccgctcct
     3181 cgttccacgg gtcggcctgg ttgtggtacc cccgctcctc ccagaagccg cggcggtcgc
     3241 gcgtcatgag ctccaggccc ttcacccact tcgcgctctt ccaggcgtag cgcttgggca
     3301 ccaccaggcg cagcggcgcg ccgtgctccg gcgtgagcgg ctcgccgttg cgcgcccagg
     3361 cgaacagcac gtcgtcgtcc agcagcgcct ccagcggcag gttggccgag tacccgtcgt
     3421 gggaccacac catggcgtgg gagacctcgg gatccggcgt caccagctcg tagacggtgc
     3481 ggaaggccac gccctcccag tcgttgtcga acacgctgaa cttggtgacg cagtggaagt
     3541 cggcgcgcac gccggcgcgc ggcagcgcct tgaactcgtc caaggtgagc tcgcgctcgc
     3601 gctcgaccgc gccgaagatg cggaagcgcc aggtggccag gtccacgcgc ggcacggcgc
     3661 cgaagtgcag gaccggccag ccgcgcgcct cgtactgtcc ggggggcagc cggtcggcgt
     3721 tcgcgctcat gcggcggagc ctacccaagg cccggccccg gcagcgtggc gcgcagcgcg
     3781 tcggcggccc gctcgcagtc ggagcggctg acgtcgcggt gcgtgaccag gcggaccgtg
     3841 cgcgggtcca tcgggccggc cagcacaccg cgctcggcca gcgccgcgat gaccgccgga
     3901 gcgtccacgc cctcgatgct gacgatgttg gtctgcacgc gcgccgggtc gatgccgtcc
     3961 gggtgcacgt cagcggcggc ccgcgccagg acctgcgcgt tctcgtggtc ctcgtgcagc
     4021 cggtcgacgt cgcgcagcgc gaccaggccc gccgccgcga tcaccccggc ctggcgcatg
     4081 ccgccgccgt agcgctggcg ccacagccgg gcctgctcga tgtgggcggc gtcgcccacc
     4141 atgagcgagc cgaccggcgc gcccaggccc ttggacaggc agaacatcag gccgtccacc
     4201 tcggcggcga actcggcggc cggggtggcg gtggcggcgg cggcgttgaa cacgcgggcg
     4261 ccgtccatgt agagcgcgac gccggcctcg gcggtcagct tccgcagccc gcgcagctcg
     4321 tccagcgtgt agcaggtccc gccgccgcgg ttgtgcgtct gctccaccca caccagggtg
     4381 gtgggcgtga gcgggaacac gtccggcttg agggcggcct gcacctggtc ggcggccagg
     4441 atgccgcggc gctgagggtc ggcggccgac agggtgcgga actgcacccc ggccaggatc
     4501 gcgcccgcgc cggcctcgta gttgacgagg tggctgtcgc cctcgaccac cacttcggtg
     4561 ccggggcggg cgagcaggcg cagccagatc tggttgcaca tcacgcccgt ggggcagtac
     4621 agcgcggcct cgcggccgag cagggcggcg gcctgctcct ccaggcggcg gaccgtgggg
     4681 tcctcgcggt agacctcgtc gcccacctcg gcctcggcca tggcccgtcg catctgtggc
     4741 gtgggcttcg tcacggtgtc gctgcgcaga tcgatcatgc cgccgatcct gccacgaccg
     4801 cgctcccctg acgcctcccc gcgtcgtacg ttgacacgcg gacgcctggg agggacatgc
     4861 catgtgggca agacggcgcg cgaggccgcc atcggcggcg atgctgacca gggacgggcg
     4921 gggtgcgccc gtccacgccg aactggtgga ggacgtcacg ctggtgcgcg acctgacgcc
     4981 ggcgcggtgg gtggaggagt cgatggtgcc gtggcccccg cgcgaggcgg gcctggcggc
     5041 gccggtgggc agcgtggtcc ccgcgtgctt cgccgcgtac ctgcgcgtcc accaccgcgg
     5101 cggcgcggac gggctggcac ccgaccaggc gggggccctg ctggaggtgc tgctgcggca
     5161 caccgcgacg ggcgacgccg gctggatctg cgtgtgggag ggctggggcg gctcgccgcc
     5221 cgaattcgac gcggcgccca aggtctccac gcccgcgcgg gcccacgtcc tgttgcgcgg
     5281 cagcctgcgc cttgtcgaga cgcggctgat gcgcgacccc ttcgggcggc actcgttcga
     5341 cccgagcttg ttctggccgg acgaccgggc ctggtgcgtc gcctcggaca cggacctggc
     5401 gtggtcgtac gtcggcgggt ccgccgactg catcgcggct cttgccgcgg tctttcccga
     5461 cgcggtgacg gtgcgccccg aggaccccgt cgtctgacgg tgcgctcgtg tccggccggc
     5521 cgtttcgtcg ccaaggcatc tttcccacag attcaatttc gtgggttgac ctgtcgcagt
     5581 cgcgttgtat ggtatcgaac atgtgttcga gtgcggggtt cgagatcgac gcggcgctgg
     5641 ccaccgcgga ccttcacggt ctgctggacg tggccgagcg cgtgttgcgt cgcgcgaagg
     5701 cgctgccgct gggcggcgcg gtcgacgatg tggcgttgac ccaggccgtg cagcgcctgg
     5761 aggcgctcag ccgcgtcgcg gagtcgcaca agtgcgcggt cgtgggcgag atcgagcagc
     5821 gccacgccgc ccgcggccag tccgccgggt cgaccgagga cctgttgacg cgcgtgctgc
     5881 acctgacacc gggtgaggcg agggcgcgca cggagctggc ggcggggctg aagctgttgc
     5941 cggacacggc ggcggcgttc gccgacggcc gcatgggcgt gagccaggcc gccgtggcgg
     6001 tgcgcaaagc cgccgaagtg gccggccgtg acgacgccga ggtgctgctc gccaccattg
     6061 accggaccgc gtcgacggtc ggccagaccg tgaaccgcac ccgcctggcc cgcgagatcg
     6121 acaccgcggt cgccaaaacc ggtgtggatg tgttggcgga gcgggaacgc cgcgcgtttc
     6181 aggaccgcaa gctccaatgg tccgtg
//
LOCUS       DATMKC010000261         4827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_169700, whole genome shotgun sequence.
ACCESSION   DATMKC010000261 DATMKC010000000
VERSION     DATMKC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169700"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VNU01_07585"
     CDS             <1..1116
                     /locus_tag="VNU01_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32518.1"
                     /translation="PVRRFDVAAVEVPAPMLGRRSGRVFATADAADEVAAGSREPEPL
                     VLRVTVGDCVEVALENRLPDARASFRTDLLAADPAVSAGVAAGRMPDQSVPPGAVRTY
                     RFHASPEVGETVAVVRDGADVLGNVSRGMYGAIVVAPAGSTFHDPVDGRELGARSSWR
                     ADVRPPDAAPYRDAVLVLHDEDEAIGSHRMPYTEDVDGAVAVNYRSEPLRGRSIAAAV
                     GDGPATPVIDAVAGDEVVLHVVAPWSQQVQVFSVEGHRWVEEPSPAGEPVPADAPRLS
                     SRRVGGLQALTLRLEHGAGGLERLPGDYLYGNHRVPYQEAGAWGVLRVHPRAGDGLRS
                     LKAGRGALATAALGAVALLPVALWWRRRRRAASAAAS"
     gene            complement(1238..2161)
                     /locus_tag="VNU01_07590"
     CDS             complement(1238..2161)
                     /locus_tag="VNU01_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWH32519.1"
                     /translation="MSGPVQPKDRECHDEPLHGVETREGRHTEVGGLPVTRVLPTKSR
                     RTVGSWCFVDLAGPADAERPDPMEVGPHPHIGLATVTWLLEGEALHSDSLGTQQVIRP
                     GQLNLMTAGHGIAHAELAAKPPFRGVQMWLAQPEATRHGASRFAHHADLPRVAFGDGE
                     GLLFAGELDGAASPAVTDTRVLGLDLAVGRGVTVLPAETGFEYAVVPLDGRVRVGPEV
                     VEPGWVGLVPAGLDELPLETELARSRLLVLGGEPLGEPVQMWWNFVARDKDEITVAWR
                     EWRDRTDRFGDVPSKLARIEAPRPPWVRPDD"
     gene            2282..2719
                     /locus_tag="VNU01_07595"
     CDS             2282..2719
                     /locus_tag="VNU01_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32520.1"
                     /translation="MRTMTGTAEQTIELGEPAQAAELARELLTAQADAGALVAELMER
                     PDAWLRAEPLVAAVAATARRVAGEVAEDRGDAGGALCVHGPVPAVVWAREVGATQRLL
                     RAALRRVTDHGQADRATTVALGAAAREMRAHVRALAGVNWAVV"
     gene            2792..4594
                     /locus_tag="VNU01_07600"
     CDS             2792..4594
                     /locus_tag="VNU01_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWH32521.1"
                     /translation="MALSIDVLGVPALERDGEPAAVRGHKVWGLLAYLLLTDAPPSRH
                     QLAGLLFGEADDPVGALRWNLSQLRQALGPGVELGGDPVTLTLPADTTVDAHVLTRGG
                     WVEALDLPGLGQDLLEGLGFAAAPGFELWLASERRRLNGAAAAVLHEAALNRLARSEA
                     AEAAGYAARLVALDQFDENAHVVLVRCLRAAGDFAGAERQVDACTELFTRELGVAPSS
                     ALRDAAAAAASGEPGTVGRAAVLAHLDAGEAAIRAGALDAGLAALRRAVVGARAADEP
                     DLLARVLVALGGALVHAARGSDVDGAAALHEAVALAAKSGERSLAATAQRELGYIDFL
                     RGRYDRARESLRAAAELAEGDDVALGWVEVIAGAAHSDAGEYGRAEELLRRGIERTGT
                     AGDRRGAAYGSAFLGRLHLLRGETGEARHTLNDALDVARDDAWAAFLPWPEALLAEVH
                     LGGDELDAAQAALEHALVLGEQLGDPCWVSLSTRGLGLLAARRGEHGDAIALLDEAPR
                     ACRRLPDAYLWVEAYALDALCGLAVEQAPQSARRWVAQMEELVSRTGMRELQVRSALH
                     RARLGEPGAGELAAALAAPVDNPLLHESVAALRA"
BASE COUNT          557 a         1807 c         1898 g          565 t
ORIGIN      
        1 cccgtgcgcc gcttcgacgt cgccgccgtg gaggtccccg cgccgatgct cggacggcgc
       61 agcggccggg tgttcgcgac cgcagacgcg gcggacgagg tcgccgccgg gtcacgggag
      121 cctgagccgc tggtgctgcg cgtcaccgtc ggcgactgcg tcgaagtggc gctggagaac
      181 cggctgccgg acgcgcgggc gagtttccgc acggacctgc tcgccgccga cccggccgtc
      241 tccgcgggcg tggcggcggg ccggatgccg gaccagtcgg tgccgccggg ggccgtgcgc
      301 acctaccgct tccacgcctc gccggaggtg ggggagaccg tcgccgtggt gcgcgacggc
      361 gccgacgtgc tcggcaacgt ctcccgcggc atgtacgggg cgatcgtcgt cgcgccggcc
      421 ggctcgacgt tccacgaccc ggtggacggc cgggagcttg gggcgcgctc gtcctggcgg
      481 gcggacgtcc gcccgccgga cgccgccccg taccgcgacg ccgtgctggt gctgcacgac
      541 gaggacgagg ccatcggcag ccaccgcatg ccgtacaccg aggacgtcga cggtgcggtg
      601 gcggtgaact accgcagcga gccgttacgc ggacggtcca tcgccgcggc ggtcggggac
      661 gggcccgcca cgccggtgat cgacgccgtg gcgggcgacg aggtcgtgct gcacgtcgtc
      721 gcgccgtgga gccagcaggt gcaggtcttc tccgtggaag gacaccgctg ggtggaggag
      781 ccgtcgcccg cgggcgagcc ggtgcccgcc gacgcgcccc ggctgtcgtc ccgccgcgtg
      841 ggcggcctgc aggcgctgac gctccggctc gagcacggcg ccggcgggct cgagcggctg
      901 ccgggcgact acctctacgg caaccaccgg gtgccctacc aggaggccgg ggcatgggga
      961 gtgctgcgcg tgcacccgcg cgccggtgac ggtctgcgtt cgctcaaggc tgggcgggga
     1021 gcgctggcga ccgccgcgct cggcgccgtc gcgctgttgc ctgtcgcgct gtggtggcgc
     1081 cgccgccgga gagctgcttc ggccgcggca tcctgaccgc cgagcgcgag cggtagcgcc
     1141 ggcgcgtccc cggaactgcc gctgaacggc acagggcgac gcctgccgcc tcaacccgaa
     1201 cccccgacgt gtcgccatca ctcggggata ggacaaccta gtcgtcgggg cgcacccacg
     1261 gcgggcgcgg ggcctcgatg cgcgcgagct tcgacggcac gtcgccgaag cggtccgtgc
     1321 ggtcgcgcca ctcgcgccac gccacggtga tctcgtcctt gtcgcgcgcg acgaagttcc
     1381 accacatctg caccggctcg cccagcggct cgccgcccag caccagcagg cggctgcgcg
     1441 ccagctcggt ctccagcggc agctcgtcca ggccggccgg caccaggccc acccagccgg
     1501 gctccaccac ctccggcccc acgcgcacgc gtccgtccag cggcaccacc gcgtactcga
     1561 agccggtctc ggcgggcagc accgtcacgc cgcgccccac cgccaggtcc agtcccagca
     1621 cgcgcgtgtc ggtgacggcg ggggaggccg cgccgtccag ctcgccggcg aacagcaggc
     1681 cctcgccgtc gccgaacgcg acccgcggca ggtcggcgtg atgggcgaag cgcgacgccc
     1741 cgtggcgtgt cgcctcgggc tgcgccagcc acatctgcac gccgcggaag ggcggcttgg
     1801 cggccagctc ggcgtgcgcg atgccgtgcc cggccgtcat caggttgagc tggccgggcc
     1861 ggatcacctg ctgggtgccc agcgagtcgg agtgcagcgc ctcgccctcc agcagccacg
     1921 tgaccgtggc caggccgatg tgcgggtgcg ggcccacctc catggggtcg ggccgctcgg
     1981 cgtccgcggg gcccgcaagg tcgacgaagc accacgaccc cacggtgcgc cggctcttgg
     2041 tcggcagcac ccgcgtcacc ggcaggccgc cgacctcggt gtggcggccc tcgcgcgtct
     2101 ccacgccgtg cagcggctcg tcgtggcact cacggtcctt gggctgcacc ggtccgctca
     2161 tgggcgtctc ctcgaaaaaa gttccagaaa cgttcggcgt cacacgggcc gtcacacgcc
     2221 tggggcccat cgtctccctc agcacaccac cacaccaggg ccaaaacagg agaaaacgcc
     2281 gatgcgcacg atgacgggga ccgccgagca gaccatcgaa ctgggagagc cggcccaggc
     2341 cgcagagctc gcccgcgagc tgctgaccgc ccaggccgac gccggggccc tggtggccga
     2401 gctgatggag cgcccggacg cctggctgcg ggccgagccc ctcgtcgcgg ccgtcgccgc
     2461 gaccgcacgc cgggtcgccg gcgaggtcgc cgaggatcgc ggtgacgcgg gcggcgcgct
     2521 gtgtgtgcac ggccccgtgc ccgcggtggt gtgggcccgc gaggtcggcg ccacccagcg
     2581 gctgctgcgc gcggcgctgc gccgcgtgac cgaccacggg caggccgacc gggccaccac
     2641 cgtggcgctc ggcgccgccg cccgcgagat gcgcgcgcac gtgcgggcgc tggcgggcgt
     2701 gaactgggcg gtggtctagg tgcccggcgc ggtcccacgc gccggcgccc cgtcggtcac
     2761 actgggccaa ggcggagggg aaggacggcc gatggcgctg tcgatcgacg tgctgggtgt
     2821 gccggcgctg gagcgggatg gcgagccggc cgccgtgcgc ggccacaagg tgtgggggct
     2881 gctcgcctac ctgctgctca ccgacgcccc gcccagccgc caccagctcg cggggctgct
     2941 gttcggcgag gccgacgacc ccgtgggcgc gctgcgctgg aacctgtcgc agctgcggca
     3001 ggcgctgggc ccgggcgtcg agctgggcgg cgaccccgtc acgctgacgc tgccggcgga
     3061 cacgaccgtg gacgcgcacg tcctgacgcg cggcggctgg gtggaggcgc tggacctgcc
     3121 cggcctcggc caggatctgc tggagggcct cggtttcgcc gcggcgcccg gcttcgagct
     3181 gtggctggcg tcggagcgcc gccgcctgaa cggtgcggcg gccgccgtgc tgcacgaggc
     3241 cgcgctcaac cgcctggccc gcagcgaggc ggcggaggcg gccggctacg ccgcgcggct
     3301 ggtcgccctg gaccagttcg acgaaaacgc ccacgtggtg ctggtgcgct gcctgcgggc
     3361 cgccggtgac tttgccgggg ccgagcggca ggtggacgcc tgcacggagc tgttcacccg
     3421 ggagctcggt gtggcgccca gctccgcgct gcgcgacgcg gcggcagcgg cggcgtcggg
     3481 cgagccgggc acggtcgggc gcgccgcggt gctggcgcac ctggacgccg gcgaggccgc
     3541 gatccgcgcg ggcgcgctgg acgcggggct cgccgcgctg cgccgcgccg tcgtcggcgc
     3601 ccgcgccgcc gacgagccag acctgctcgc gcgcgtcctg gtggcgctgg gcggcgcgct
     3661 ggtgcacgcc gcccgcggca gcgacgtgga cggcgcggcc gccctgcacg aggccgtcgc
     3721 gctggccgcc aagtccggcg agcgcagcct cgccgcgacc gcgcagcgcg agctcggtta
     3781 catcgacttc ctgcgcggcc gctacgaccg cgcccgcgag tcgctgcgcg cggcggcgga
     3841 actggccgag ggcgacgacg tggcgctcgg ctgggtggag gtcatcgcgg gcgcggccca
     3901 cagcgacgcc ggcgagtacg ggcgcgcgga ggagctgctg cgccgcggca tcgagcgcac
     3961 cggcacggcg ggcgaccggc gcggcgcggc gtacggcagc gccttcctgg ggcgcctgca
     4021 tctgctgcgc ggcgagaccg gcgaggcgcg ccacacgctg aacgacgcgc tcgacgtcgc
     4081 gcgcgacgac gcgtgggccg cgttcctgcc gtggccggag gcgctgctgg cagaggtcca
     4141 cctgggcggg gacgagctcg acgccgcgca ggcggcgctg gagcacgcgc tggtgctggg
     4201 cgagcagctt ggcgacccgt gctgggtgag cctgagcacg cgcggcctcg gcctgctggc
     4261 ggcgcggcgc ggcgagcacg gcgacgccat cgcgctgctg gacgaggcgc cccgggcctg
     4321 ccgccgcctg cccgacgcct atctgtgggt ggaggcttac gcgctggacg cgctgtgcgg
     4381 gctggccgtg gagcaggcgc cgcagtcggc gcggcgctgg gtcgcgcaga tggaagaact
     4441 ggtgagccgg accggcatgc gcgagctgca ggtgcgcagc gcgctgcacc gggcgcggct
     4501 gggcgagccc ggcgccggtg agttggccgc cgcgctggcc gcccccgtcg acaacccgct
     4561 gctgcacgag tcggtcgccg cgctgcgagc ctgaccccgc cgctccggga ccacttgtgg
     4621 ccgatagcgg acccaactcg tcccggaacc gggggggctc agtcgcccgc gacgaagaac
     4681 agccagcgcc cgtcggcgcc gatgccggcc aggaaacgca tgggctcgcg gccgtcggac
     4741 tcctggcggc gccagtaggc gatcgggtcg tcctgctcgc cgaaggagta ggtgaagccc
     4801 tcgcccggcg agctcgttca ggcggtc
//
LOCUS       DATMKC010000262         6362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_51581, whole genome shotgun sequence.
ACCESSION   DATMKC010000262 DATMKC010000000
VERSION     DATMKC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51581"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3584
                     /locus_tag="VNU01_07605"
     CDS             <1..3584
                     /locus_tag="VNU01_07605"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HWH32522.1"
                     /translation="LADPSSVAESWQEFFSDYTPSWEAGGTRGITPSQAATAAPARPA
                     AAQPAQAPAKAPAAKAAAPEGNGQAPDGATQIRGVAATIAQNMQESLAVPTATSIRVV
                     PAKLLEVNRTIVNNQLRRTRGGKVSFTHLIGWAVVKAMAEMRVMTSSYHEADGKAYVH
                     RPEHFNLGLAVDVERKDGGRTLLVPNIKQADALDFAGFFRAYEDVIRKIKANELSPEL
                     FADTTATLTNPGTVGTVGSVPRLMPGQSVIVGVGAIDYPAEYQAADPRTLAEIGVGKV
                     ITLTSTYDHRVIQGAESGLFLQRIHELLLGQHDFYDEIFASLSIPYEPVRWRADDHSR
                     IAASATDNRYDKQVAVQQVINMYRVRGHLIANLNPLQHTFQRVHSELDPATYGLTIWD
                     LDREFATGGLAGRDRMQLSRILGLLRDAYCRTIGVEYMHMQEPEQKAWIQEQVEGVTY
                     TFTPEEQRRILEKLNAAEAFEKFLHTKYIGHKRFSLEGAESLIAMLDAVLNEAADNGV
                     EEAVMGMAHRGRLNVLTNVMGKDYNKIFREFEGDLDPESVQGSGDVKYHLGQYGKHTS
                     PAGNTIGVTLSSNPSHLEAVDPVVEGMVRAKIDQMPGATAGPGAPVLPVLVHGDAAFA
                     GQGVVAETLAMSQLRGYRTGGTVHVVVNNQVGFTTSPMSARSSTYATDVAKMVQAPIF
                     HVNGDDPEACVRVMRLAFAFRQAFGKDVVVDLICYRRWGHNEGDDPSYTQPLMYKAIE
                     ARRSVRKLYTEELVNRGDLTLEEAEQALDDFRQRLENALKQTRESSPPDAAAVDLGTV
                     PSGGVAPRVETGVERSVLDRVAEAVTSWPESFNPHPKLAKQLEKRRSMLDQDAADWAM
                     GEALAFGSLLLEGHPIRLAGQDSRRGTFSQRHSALIDYETAAEHIPLANLSEGQAPFM
                     TYDSLLSEFAAMGFEYGYSVARPDALVLWEAQFGDFVNGAQVIIDQFLVAAEDKWGQR
                     SGLVLLLPHGYEGQGPEHSSARIERFLTLSAEGNLQAVQPTTSAQYFHVLRRQMVADV
                     RKPLVVFTPKALLRAKPAASAAAEFASGSFREVLPLDTGVAAQDVRRVLLCSGKVAYD
                     LAAAREERQAPAAIVRVEQLYPWPEQQIADAVAAFPNVKDVYWVQDEPENMGPWPFAH
                     GRLHSILGKQLPLRHASRHESASPATGSAAMHEAEQRQLMEDAFSGL"
     gene            3595..4887
                     /locus_tag="VNU01_07610"
     CDS             3595..4887
                     /locus_tag="VNU01_07610"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HWH32523.1"
                     /translation="MTDADRDLGQDLCRFVDAGPSPYHAVAEMARRLEAAGFSELDER
                     EHWALAPGDRRFVVRDGGSVAAFVVGSAAPSDAGFRMVGAHTDSPTFAVRPLPDVRRG
                     GFALVGVEPYGGVLAHTWFDRDLTVAGRLAVRTASGITTALVRLPGAPLRIPNLAIHL
                     FRELREEGFKPDPQQHAVPVWGFDSTGALLDALAGHAGVDPGDVLGHDLVLADTQPAA
                     LGGADDAFVLAPRLDNLASCHAALHALTAAGPGRATAVLVANNHEEIGSASAEGAQGS
                     FLEDVLRRVVSATEGDDGQRFAMACARSRLVSSDMAHAVHPNYSDRHEPGHTPRLGGG
                     PVLKSNANQSYATDAVGTAHFRAACAEAGVAVQHFATRADLPCGSTIGPLTATRLGVA
                     TVDVGNPLLSMHSVREQAAAADIAPMAAALRAHLSADE"
     gene            4880..5935
                     /locus_tag="VNU01_07615"
     CDS             4880..5935
                     /locus_tag="VNU01_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWH32524.1"
                     /translation="MSEPSGAGRPLTQRPFVRVGTYAWAFVGMVVVLVVLGVVLAELT
                     VVWVPLLLALFPAALLAPVNQRLRRVVPRWAAALVTLLGFLGLLILVVWGLVPVVAGE
                     LPALGESLRQAVDGLRDFAERSGVNVSPERIDAVIEQTRDRALSTLRGSATEILTAVA
                     EGVTGLLLAVLALFFYLKDGERIGAWLRSLFPERARDDAEAIGDSTWWTLGAYFRGQL
                     FIAFVDAVFIGIGIALLGVPLALPLAVLVFFGGLFPIVGAFLSGGVAVLVALADGGPV
                     IALAVLGVVVGVQQLESNILEPVVLSRAVHLHPLAVIVALATGGVLLGVLGAFLAVPL
                     AASGARAVAHLRARTPG"
     gene            complement(5939..>6362)
                     /locus_tag="VNU01_07620"
     CDS             complement(5939..>6362)
                     /locus_tag="VNU01_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32525.1"
                     /translation="QPSFGGTTPPPPMPTRNDPNSVAIGRAGLGHDAAMWLQQVADQV
                     DARLDRISATWRTSPHAEAARACAFGLLLGHLARLYPHMRPELNATAELHPSFSTLLE
                     GSRLATLEQIVAEPGRMAAWLGPLIDVQDPDRMRMLLD"
BASE COUNT          870 a         2320 c         2316 g          856 t
ORIGIN      
        1 acctcgccga tccctcgtcc gtggccgaga gctggcagga gttcttctcc gactacaccc
       61 ccagctggga ggccggcggc acgcgcggga tcacgccgtc gcaggccgcg accgccgcgc
      121 cggcacgccc cgcggcggcg cagccggcgc aggcgccggc caaggctccc gccgccaagg
      181 ccgcggcgcc ggagggcaac ggccaggcgc ccgacggcgc cacgcagatc cgcggcgtcg
      241 ccgccaccat cgcccagaac atgcaggagt cgctcgcggt gcccaccgcg acgtccatcc
      301 gcgtggtgcc ggccaagctg ctcgaggtca accgcaccat cgtcaacaac cagctgcggc
      361 gcacgcgcgg cggcaaggtc agcttcacgc acctgatcgg ctgggccgtg gtgaaggcca
      421 tggcggagat gcgcgtcatg acctccagct accacgaggc agacggcaag gcgtacgtgc
      481 accggcccga gcacttcaac ctgggcctgg ccgtggacgt ggagcgcaag gacggcggcc
      541 ggacgctgct ggtgcccaac atcaagcagg ccgacgcgct ggacttcgcc gggttcttcc
      601 gcgcctacga ggacgtcatc cgcaagatca aggcgaacga gctgagcccg gagctcttcg
      661 ccgacaccac cgccacgctg accaaccccg gcacggtggg caccgtgggc tcggtgccgc
      721 ggctcatgcc gggccagtcc gtcatcgtcg gcgtgggcgc gatcgactac cccgcggagt
      781 accaggccgc cgacccgcgc acgctggcgg agatcggcgt cggcaaggtc atcacgctca
      841 cgtcgacgta cgaccatcgc gtgatccagg gcgcggagag cgggctgttc ctccagcgca
      901 tccacgagct gctgctgggc cagcacgact tctacgacga gatcttcgcc tcgctgagca
      961 tcccgtacga gccggtgcgc tggcgtgcgg acgaccactc gcgcatcgcg gccagcgcca
     1021 cggacaaccg ctacgacaag caggtcgccg tccagcaggt catcaacatg taccgggtgc
     1081 gcgggcacct gatcgccaac ctcaacccgc tgcagcacac gttccagcgc gtgcacagcg
     1141 agctggaccc ggcgacgtac ggcctgacca tctgggacct ggaccgcgag ttcgccaccg
     1201 gcgggctcgc tggccgcgac cgcatgcagc tgtccaggat cctcggcctg ctgcgcgacg
     1261 cgtactgccg caccatcggt gtggagtaca tgcacatgca ggagccggag cagaaggcgt
     1321 ggatccagga gcaggtggag ggcgtcacct acaccttcac gccggaggaa cagcggcgga
     1381 tcctggagaa gctgaacgcc gccgaggcgt tcgagaagtt cctgcacacc aagtacatcg
     1441 ggcacaagcg cttcagcctc gaaggcgccg agagcctgat cgcgatgctg gacgcggtcc
     1501 tcaacgaggc ggcggacaac ggcgtcgagg aagccgtcat gggcatggcc caccgcgggc
     1561 gcctgaacgt cctgaccaac gtgatgggca aggactacaa caagatcttc cgcgagttcg
     1621 agggcgacct ggacccggag agcgtccagg gctccggcga cgtgaagtac cacctgggcc
     1681 agtacggcaa gcacacctcc ccggccggca acaccatcgg cgtgacgctg tcgtccaacc
     1741 ccagccacct cgaggcggtc gaccccgtgg tcgagggcat ggtgcgcgcc aagatcgacc
     1801 agatgccggg cgcgaccgcc ggtcccggcg cacccgtgct gccggtgctg gtgcacggcg
     1861 acgccgcgtt cgccggccaa ggcgtggtcg ccgagaccct ggcgatgagc cagctgcgcg
     1921 gctaccgcac cggcggcacc gtgcacgtgg tggtcaacaa ccaggtcggt ttcaccacct
     1981 cccccatgtc ggcgcgctcc tccacctacg ccaccgacgt ggccaagatg gtccaggcgc
     2041 cgatcttcca cgtcaacggc gacgaccccg aggcgtgcgt gcgggtcatg cgcctggcgt
     2101 tcgccttccg ccaggcgttc ggcaaggacg tcgtggtcga cctgatctgc taccgccgct
     2161 ggggccacaa cgagggcgac gacccgagct acacccagcc gctcatgtac aaggccatcg
     2221 aggcgcgccg cagcgtgcgc aagctgtaca ccgaggagct ggtcaaccgc ggcgacctca
     2281 cgctggagga ggccgagcag gcgctcgacg acttccgcca gcggctggag aacgcgctga
     2341 agcagacgcg cgagtcctcg ccgccggacg ccgccgccgt ggacctgggc accgtgccca
     2401 gcggcggggt ggcgccgcgc gtcgagaccg gcgtggagcg cagcgtgctc gaccgcgtcg
     2461 cggaggccgt gacctcctgg cctgagagct tcaacccgca cccgaaactg gccaagcagc
     2521 tggagaagcg ccgctcgatg ctggaccagg acgccgccga ctgggcgatg ggcgaggcgc
     2581 tcgcgttcgg ctcgctgctg ctggagggcc accccatccg gctggccggc caggacagcc
     2641 ggcgcggcac gttcagccag cgccacagcg cgctgatcga ctacgagacc gccgcggagc
     2701 acatcccgct ggcgaacctg tcggagggcc aggcgccgtt catgacctac gactcgctgc
     2761 tcagcgagtt cgccgccatg ggcttcgagt acggctactc ggtggcccgc cccgacgcgc
     2821 tggtgctgtg ggaggcgcag ttcggcgact tcgtcaacgg cgcccaggtc atcatcgacc
     2881 agttcctggt ggccgccgag gacaagtggg ggcagcgctc cggcctggtg ctgctgctgc
     2941 cgcacggcta cgagggccag ggcccggagc acagctcggc gcgcatcgag cgcttcctga
     3001 cgctgtcggc cgagggcaac ctgcaggcgg tgcagcccac gacgtccgcg cagtacttcc
     3061 acgtgctgcg ccgccagatg gtcgccgacg tgcgcaagcc gctggtcgtg ttcacgccga
     3121 aggcgctgct gcgggccaag cccgccgcca gcgccgcggc ggagttcgcc tccggcagct
     3181 tccgggaggt actcccgctg gacaccggcg tggccgcgca ggacgtgcgc cgcgtgctgc
     3241 tgtgcagcgg caaggttgcc tacgacctgg ccgccgcccg cgaggagcgc caggccccgg
     3301 cggccatcgt gcgcgtggag cagctgtacc cgtggcccga gcagcagatc gccgacgcgg
     3361 tcgcggcgtt ccccaacgtg aaggacgtgt actgggtgca ggacgagccg gagaacatgg
     3421 ggccgtggcc gttcgcgcac ggcaggctgc actcgatcct gggcaagcag ctgccgctgc
     3481 gccacgccag ccggcacgag agcgccagcc cggccaccgg ctccgcggcg atgcacgagg
     3541 ccgagcagcg ccagctcatg gaggacgcgt tctccggcct ttaggctcgc ggcgatgact
     3601 gacgccgacc gcgacctcgg gcaggacctg tgccgcttcg tcgacgccgg gccctcgccg
     3661 taccacgcgg tcgcggagat ggcgcgccgc ctggaggccg ccggcttctc ggagctggac
     3721 gagcgcgagc actgggccct cgcgccgggt gaccggcgct tcgtggtccg cgacgggggc
     3781 tccgtcgccg cgttcgtcgt gggctccgcc gcccccagcg acgccggctt ccgcatggtc
     3841 ggcgcgcaca ccgactcgcc caccttcgcc gtccggccgc tgccggacgt gcggcgcggc
     3901 ggcttcgcgc tggtcggcgt ggagccgtac ggcggcgtcc tggcgcacac ctggttcgac
     3961 cgggacctca ccgtcgccgg gcggctggcc gtgcgcaccg cctcgggcat caccaccgcg
     4021 ctcgtgcgcc tgccgggcgc gccgctgcgc atcccgaacc tggcgatcca cctgttccgg
     4081 gagctgcgcg aggagggctt caagccggac ccgcagcagc acgcggtccc ggtgtggggc
     4141 ttcgacagca ccggtgcgct gctggacgcg ctggccggcc acgccggcgt ggatcccggc
     4201 gacgtgctgg gccacgacct ggtgctggcc gacacgcagc cggccgcgct cggcggcgcc
     4261 gacgacgcgt tcgtgctggc gccgcggctg gacaacctcg cctcctgcca cgccgcgctg
     4321 cacgcgctga cggcggccgg gccggggcgg gccacggccg tgctggtggc caacaaccac
     4381 gaggagatcg gctcggcgag cgccgagggc gcgcagggca gcttcctgga ggacgtgctg
     4441 cgccgcgtgg tcagcgccac cgagggcgac gacgggcagc gcttcgcgat ggcctgcgcg
     4501 cgctcgcgcc tggtgtccag cgacatggcg cacgccgtgc accccaacta cagcgaccgg
     4561 cacgagcccg gccacacgcc gcgcctgggt ggcgggccgg tgctcaagag caacgcgaac
     4621 cagtcctacg ccaccgacgc ggtcggcacg gcccacttcc gcgccgcgtg cgccgaggcg
     4681 ggggtggcgg tgcagcactt cgccacgcgc gccgacctgc cgtgcggctc gaccatcggc
     4741 ccgctcaccg cgacccggct gggggtcgcc accgtcgacg tgggcaaccc cttgctgtcc
     4801 atgcactccg tccgggagca ggcggccgcc gccgacatcg cgccgatggc cgccgcgctg
     4861 cgcgcccacc tgagcgccga tgagtgagcc ctccggcgcc ggccggccgc tgacccagcg
     4921 gccgttcgtc cgcgtcggca cgtacgcgtg ggccttcgtc ggcatggtcg tggtcctggt
     4981 ggtgctcggc gtggtcctgg ccgaactcac ggtggtgtgg gtgccgctgc tgctggcgct
     5041 gttccccgcg gccctgctgg cgccggtgaa ccagcggctg cgccgcgtgg tgccccgctg
     5101 ggcggcggcg ctcgtcacgc tgctcgggtt cctgggcctg ctgatcctgg tggtgtgggg
     5161 gctggtgccc gtggtcgccg gggaacttcc ggcgctgggc gagtcgctgc ggcaggccgt
     5221 ggacgggctg cgcgacttcg ccgagcggtc cggcgtgaac gtctcgcccg agcgcatcga
     5281 cgcggtgatc gagcagacgc gcgaccgggc gctgtcgacg ctgcggggca gcgccacgga
     5341 gatcctcacc gccgtcgccg agggcgtcac cggcctgctg ctggcggtgc tggcgctgtt
     5401 cttctacctg aaggacggcg agcgcatcgg cgcctggctg cgcagccttt tccccgagcg
     5461 cgcgcgcgac gacgccgagg cgatcggcga cagcacgtgg tggacgctgg gcgcctactt
     5521 ccgcggccag ctgttcatcg ccttcgtcga cgcggtgttc atcggcatcg gcatcgcgct
     5581 gctcggcgtg ccgctggccc tgccgctggc ggtgctggtg ttcttcggcg gcctgttccc
     5641 catcgtcggc gcgttcctgt ccggcggcgt cgcggtcctg gtggcgctgg ccgacggcgg
     5701 gcccgtcatc gcgctggcgg tgctgggcgt ggtggtgggc gtgcagcagc tggagagcaa
     5761 catcctggag ccggtggtgc tcagcagggc ggtgcacctg cacccgctgg cggtgatcgt
     5821 ggcgctggcc accggcggcg tgctgctcgg ggtgctgggc gcgttcctgg ctgtcccgct
     5881 ggcggccagc ggggcgcgcg ccgtcgccca cctgcgggcc cggacccccg gctgagcctc
     5941 agtcgagcag catgcgcatg cggtcggggt cctggacgtc gatcagcggg ccgagccacg
     6001 cggccatgcg ccccggctcg gccacgatct gctcaagcgt cgccagccgg ctgccctcca
     6061 ggagcgtgga gaagctcggg tgcagctccg cggtggcgtt gagctccggg cgcatgtgcg
     6121 ggtacagccg cgccaggtga cccagcagaa ggccgaaggc gcacgccctg gccgcctccg
     6181 cgtgcggtga ggtgcgccac gtcgcgctga tgcggtcgag ccgcgcgtcg acctggtcgg
     6241 ccacctgctg cagccacatg gcggcgtcgt ggccgagccc ggcccggccg atcgcgacac
     6301 tgttggggtc gttgcgtgtg ggcatgggcg gcgggggggt cgtgccgccg aaggacggct
     6361 gc
//
LOCUS       DATMKC010000263         9220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_66189, whole genome shotgun sequence.
ACCESSION   DATMKC010000263 DATMKC010000000
VERSION     DATMKC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66189"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..666
                     /locus_tag="VNU01_07625"
     CDS             262..666
                     /locus_tag="VNU01_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWH32526.1"
                     /translation="MRRPPAPVVSVVALALLVAGCGSPEPAASDGHDHAENSPVVDGA
                     RELAVTGKDLRFTPARLEVAAGEDVTIVFTAADVAHDFTIEGAGVHVGAAAGQTARGG
                     LRIDEPGTYTFVCTVAGHATAGMTGTLTVTEA"
     gene            663..2660
                     /locus_tag="VNU01_07630"
     CDS             663..2660
                     /locus_tag="VNU01_07630"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HWH32527.1"
                     /translation="MSMEGHAGHAGHVDHAGHVDHATMFRAKFWLALALSIPVVATSH
                     MVQEWFGYHLAFPGAGWVAPLLGTVVFLVGGSPFLKGGLGELRGRRPGMMLLISLAIS
                     VAFLASLATTAGLLELDFWWELVLLIDVMLLGHWLEMRAIGQARGALRALAELLPDDA
                     ELVRDGEVVTVPIADLRAGDVVLVRPGARVPADGTVVEGAAAVDESMITGESRPVLRG
                     EGARVVAGTVATDSALRVRVEAVGEETALAGIQRLVAEAERSLSRAQVLADRAAALLF
                     YVAVTAGVLTLSVWWGIGRPDAAVTRTVTVLVIACPHALGLAIPLVTSIATSRAAREG
                     LLIKDRMALERMRTVDTVLFDKTGTLTRGRHALAGVAAADGDEDGALALAAAVEADSE
                     HPLARAIVAAARERGLRVPAASGFRSNPGLGVEATVGGRDLALGGPNLLRERGLALPE
                     SLRPHTDRWSERGAAVLALVQGDRVLAVLSTEDEVREESREAVAALHDLGVRTVLITG
                     DARQVADAVAAELGIDEVFAEVLPQDKDAAVRALQERGERVAMVGDGVNDAPALARAD
                     VGIAIGAGTDVAIESAGVVLASDDPRAVVAVLRLSRASYRKMLQNLWWAAGYNVVAIP
                     LAAGVLSWAGIDIAPALGAVLMSVSTIVVAANAQLLRRAEL"
     gene            2722..3642
                     /locus_tag="VNU01_07635"
     CDS             2722..3642
                     /locus_tag="VNU01_07635"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HWH32528.1"
                     /translation="MQLRYQADRLHHAEELDRGVGVMELLTARLFRRDRRLLDALHGT
                     WLGHPLHPVLVDLPIGFWTSSMVLDLLGGERTAAASRQLVGAGVLAALPAALTGLKDW
                     DSLGQERAAKRVGYVHAVNNGVALAAYALSWLARRRGSRGAGVALGVVGSAFATMGAY
                     LGGHLSYRQGIGVDHTVGESAPGGWTRVIRADELGDHELTHATAGDAHVVLYRSGEHI
                     GALAAHCSHLAGPLHEGRLVDKDGEACVRCPWHGSTFSFRDGRVVHGPATAPQPGYEA
                     RVADGWVEVKAKPVPKSSNGQIGLEALAGG"
     gene            3802..4182
                     /locus_tag="VNU01_07640"
     CDS             3802..4182
                     /locus_tag="VNU01_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32529.1"
                     /translation="MDEREKHAPDDEVGGTFGMWINKRTVEVLEIPFRHRLVAQPGGW
                     RLEALEAGKLVARFGNRPERMDLDQGDYVLSSGDDFYVSLQRRKDGRPEGPQRDRAGD
                     RPGEPGVERPLRDGAAAGSEPEVG"
     gene            4184..5389
                     /locus_tag="VNU01_07645"
     CDS             4184..5389
                     /locus_tag="VNU01_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32530.1"
                     /translation="MGLQEVLQGVLDGLAIQLTGSDTPQPGALVGAKIVPPLGELDVT
                     DVVEGDVSLTWLTKDVLFGNEELLGAPSVSAINSASTGGRPEVTDLGSLVALPGVPGL
                     VGQLAGTLPLSSGATVPVQATITWQVLDEDGRTELPPSEYRIPGGANSPTVQVAFAPG
                     THELTGSGTIPAPRRRHLSATVTLTANGIPAGPRTLPPVPVLVPSLPIPTVLAMFLHT
                     NFQARSGDDDGAVLVVVPASSPLRSVQQLTSTLSTLQGVIGNLTAFGSFAAFALGLSE
                     LVGALSAQPHVQFRAADSIGNLNDITLIQRSWYENDTEAEDELSSLILIGREGRGVRC
                     SNARGQDDDEGQFTVRTGSSLLAIIRSLHSASPAAEGGTIVVDRAPPGGWWNPDQFGD
                     ELSSITFMS"
     gene            5386..5667
                     /locus_tag="VNU01_07650"
     CDS             5386..5667
                     /locus_tag="VNU01_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32531.1"
                     /translation="MTGGRGRLTVVVPAPWCDLAVWRVRAGQAAGGVNEPIEVPAGPT
                     RVVIDADVTLPAVRAGAALRLSVDVPSGGAVRLHPPLLPYRALPDADGG"
     gene            5686..6429
                     /locus_tag="VNU01_07655"
     CDS             5686..6429
                     /locus_tag="VNU01_07655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH32532.1"
                     /translation="MVEGTGAQGPRAALVTGGSSGIGLALARMLGEEGYALTVSARRE
                     DKLKAAAEELRAEGLEVIEVAANLGEEDEVQRVVAAHRERYGRLDVLVNNAGVGIGAN
                     VAEISTKKMDLQLNLNLRSAILFYRDCMDMLRASAAEHRRTQVWNTASIAGKRGEAWL
                     SVYSATKAGLVGFTQAMNKELAGDGIRSTALCPAFVDTPMTDFVKGAVAADDMIRPED
                     IAELCRALLRLSPAAVIPEIMVVRPGDQM"
     gene            complement(6433..6672)
                     /locus_tag="VNU01_07660"
     CDS             complement(6433..6672)
                     /locus_tag="VNU01_07660"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HWH32533.1"
                     /translation="MDDREILSRIHELVEREEALREQGEAALDPKDRERLAALERDLD
                     QCWDLLRQRRAKRGAGQDPGEAEARPEELVERYVQ"
     gene            6756..8288
                     /locus_tag="VNU01_07665"
     CDS             6756..8288
                     /locus_tag="VNU01_07665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWH32534.1"
                     /translation="MAIHGRAEISDVATLARDVASARDQLQALLEGRWPGDVDLALIA
                     VHEALINADRHGAGIRHATARLEGDRVVVEIADYGAGFDHRGYVASQPDLLAERGRGL
                     WVIHQIACQFELVHEAEGSRVVMVFTPGTLGVQAPEPDRPPLPDVDAVAQSLIRAFGA
                     AVAIVDRNLVIRAAWGDVEGLLGLTMEDVLERDARGLTAELKTRFQDPQRYEQRILDV
                     YAQPDQVSEDVFVRRDGRIVRRHSVPFTSRGQHWRLISYIPVAEQTQVVAAVQRSLLP
                     DLPEWDGLEVAAIYHPAEASSFVGGDFYDFIQLTSGARCVVVGDVSGRGPAAAATSTH
                     VRAYLRAALRSHGVTAAVADLDATLEHELPAEHFVTLAMCTQESAGVWTHTNCGHVPA
                     LLLRDGAVKELGGRGGALGIALPAEWPREPFVLQPDDVLLLYTDGIVDAGRRERRYGA
                     ERLADSLRELGGLPPRELVRALDERVHSFAANPLADDHVLVAVRRTRPRPELDDQREE
                     PT"
     gene            8285..>9220
                     /locus_tag="VNU01_07670"
     CDS             8285..>9220
                     /locus_tag="VNU01_07670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH32535.1"
                     /translation="MTAVDWQGRLGRVLETARAHAEDVDRRARFPREVVDALGDAGLL
                     GLTVPEELGGLGGSFAEAYEVLVRLAERCASSGMILTMHYAALAVLREAGGGRFDAVL
                     REASAGRHLSTLALSEKATRSSFWISMGEAKRLPDGATRITVDKSFVTSAGPATSYVV
                     STGSAERAHLGESDLWLVSAGQQGVEVLDWWQGSGLRGNSSAPMRFDCELPPECAVCE
                     PGSGVDVILGTVVPWFQLGACAVSVGVARAAVEFARAHVTGTVLEHLGQRLVDQPVVR
                     HTLGRLFARVDSVDGFGRATARDLSDGRATMTQVLE"
BASE COUNT         1135 a         3322 c         3521 g         1242 t
ORIGIN      
        1 gcggtggcgg ccgcgaccgc gggggacgcc gcccaggagc cggccgagga cccgaccgag
       61 gacgagccca cggaggagcc gacggacgag ccgacggacg agcccacgga ggagccgacg
      121 gacgagccgc gcgagcggcc cacgcggcgg cccgcgcccc ggcggtgatc tcgtcgcgcg
      181 cgggggccgg ccggcgcgga gttgacctat accccctagg ggtataaagt cctctcgtcg
      241 tcaaccgaga ggagttccgc catgcgccgt ccgcccgctc ccgtcgtctc tgtcgtcgcg
      301 ctcgccctgc tggtcgcggg ctgcggctcc ccggagcccg ccgcgagcga cggccacgac
      361 catgcggaga actcccccgt ggtcgacggc gcgcgcgagc tcgcggtcac cggcaaggat
      421 ctgcgcttca cgcctgcccg gctcgaggtc gccgccggcg aggacgtgac catcgtgttc
      481 accgccgcgg acgtggcaca cgacttcacc atcgagggcg ccggcgtgca cgtcggcgcc
      541 gcggcgggcc agaccgccag gggcgggctg cgcatcgacg agccgggcac gtacacgttc
      601 gtctgcacgg ttgcgggtca cgccacggcg ggcatgacgg gcacattgac cgtcacggag
      661 gcatgagcat ggagggccac gccggccacg ccggccacgt ggaccatgcc ggccacgtgg
      721 accacgccac gatgttccgt gcgaagttct ggctggcgct cgcgctgtcg atcccggtgg
      781 tcgcgaccag ccacatggtc caggagtggt tcggctacca cctcgcgttc ccgggcgccg
      841 gctgggtcgc gccgctgctc ggcaccgtcg tgttcctggt cggcgggtcg ccgttcctca
      901 agggcggcct gggcgagctg cgcgggcgcc ggccggggat gatgctgctg atctcgctgg
      961 cgatctccgt ggccttcctg gcgagccttg ccaccacggc ggggctgctg gagctggact
     1021 tctggtggga gctcgtcctg ctgatcgacg tgatgctcct ggggcactgg ctggagatgc
     1081 gcgccatcgg gcaggcccgc ggcgccctgc gggcgctggc cgagctgctg ccggacgacg
     1141 ccgagctggt gcgcgacggc gaggtggtca cggtgccgat cgccgacttg cgggccggtg
     1201 acgtggtgct ggtgcggcct ggcgcgcgcg tgcccgccga cggcaccgtg gtggaggggg
     1261 cggcggccgt cgacgagtcc atgatcaccg gcgagtcacg gccggtgctg cgcggcgagg
     1321 gcgccagggt cgtggccggc accgtggcca ccgactcggc cctccgcgtc cgcgtggagg
     1381 cggtcggcga ggagacggcg ctcgcgggca tccagcgcct ggtggccgag gccgagcggt
     1441 cgctctcgcg cgcgcaggtg ctcgccgacc gcgccgccgc tctgctgttc tacgtcgccg
     1501 tcaccgccgg cgtgctgacg ctgtcggtgt ggtgggggat cggccggccc gacgccgcgg
     1561 tgacgcgcac ggtgacggtg ctggtgatcg cctgcccgca cgcgctgggc ctggcgatcc
     1621 ccctggtcac ctcgatcgcg acctcgcgcg ccgcccggga gggcctgctg atcaaggacc
     1681 gcatggcgct ggagcgcatg cgcaccgtcg acacggtgct gttcgacaag accggcacgc
     1741 tgacgcgggg gcgtcacgcg ctggccggtg tggccgccgc cgacggggac gaggacggcg
     1801 cgctggcgct ggccgccgcg gtggaggccg acagcgagca tccgctggcc cgggcgatcg
     1861 tcgcggcggc gcgcgagcgc ggcctgcgcg tgccggccgc gtcgggcttc cgatccaacc
     1921 ccggcctggg ggtcgaggcg accgtgggcg gccgggacct ggcattgggc gggccgaacc
     1981 tgctgcgcga gcgcggcctg gcactgcccg agtcgctgcg cccgcacacg gaccgctgga
     2041 gcgagcgcgg cgccgccgtg ctggcgctgg tgcagggcga ccgcgtgctc gccgtgctgt
     2101 ccaccgagga cgaggtgcgc gaggaatccc gggaggccgt cgccgcgctg cacgacctgg
     2161 gtgtgcgcac ggtgctgatc accggcgacg cccgccaggt ggccgacgcc gtggccgcgg
     2221 agctgggcat cgacgaggtc ttcgccgagg tgctgccgca ggacaaggac gcggccgtgc
     2281 gcgccctgca ggaacgcggc gagcgcgtcg cgatggtggg cgacggcgtg aacgacgcgc
     2341 cggcgctggc acgggccgac gtcggcatcg ccatcggcgc gggcaccgac gtggccatcg
     2401 agtccgcggg cgtggtcctg gcctccgacg accccagggc ggtggtcgcc gtgctgcgcc
     2461 tgtcgcgcgc gagctaccgc aagatgctgc agaacctgtg gtgggccgcc ggctacaacg
     2521 tcgtggccat cccgctggcc gccggggtgc tgtcgtgggc cgggatcgac atcgcgccgg
     2581 cgctgggcgc cgtgctcatg agcgtgtcga ccatcgtggt ggccgccaac gcgcaactgc
     2641 tgcgccgagc ggagctctga gggtttcggc gggtgcccgc gcggtgagga agcgcggcga
     2701 cgtcccaccg tggaggccgc catgcagttg cgctaccaag ccgatcgcct gcaccacgcc
     2761 gaggagctcg accgcggggt cggcgtcatg gagctcctca ccgcccgcct gttccgccgc
     2821 gaccggcgcc tgctggacgc gctgcacggc acctggctgg ggcacccgct gcacccggtg
     2881 ctggtcgacc tgcccatcgg cttctggacc tcgtcgatgg tgctggacct gctgggcggg
     2941 gagcgcaccg cggcggcgtc gcgtcagctc gtcggcgcgg gcgtgctcgc cgcgctcccg
     3001 gccgcgctca ccggactgaa ggactgggac tcgctggggc aggagcgcgc ggccaagcgc
     3061 gtcggctacg tccacgccgt gaacaacggc gtcgcgctgg ccgcctacgc gctgtcgtgg
     3121 ctggcccgcc gccgtggcag ccgcggcgcc ggcgtggccc tgggggtggt gggctccgcg
     3181 ttcgccacga tgggcgccta cctgggcggc cacctgtcct accgccaggg catcggcgtg
     3241 gaccacaccg tcggcgaatc cgcccccggg ggctggacgc gcgtgatccg ggcggacgag
     3301 ctgggcgacc acgagctgac gcacgccacc gccggcgacg cgcacgtcgt gctgtaccgc
     3361 agcggcgagc acatcggcgc gctcgcggcg cactgctccc acctcgccgg gccgctgcac
     3421 gagggccggc tggtcgacaa ggacggcgag gcgtgcgtgc gctgcccctg gcacggcagc
     3481 acgttctcct ttcgcgacgg ccgcgtggtg cacgggccgg ccacggcgcc gcagcccggc
     3541 tacgaggcgc gcgtcgccga cggctgggtg gaggtcaagg ccaagcccgt gcccaagtcc
     3601 tccaacggcc agatcggcct ggaggccctc gccggcggct gacgcgcgcg tacgcgcatt
     3661 tcccccgtgg tgctcgccac cgggggagct ttctcctccc tcgcggcggt ttccccacgg
     3721 ttgcgcattg acgattccgg gcccgtgacg atttcgtgcc tgtacgcggc gatggcatcg
     3781 ccatgtcagg agggggaagg cgtggacgag cgcgagaagc atgcgcccga cgacgaagtg
     3841 ggcggcacgt tcgggatgtg gatcaacaag cgcaccgtgg aggtcctgga gatcccgttc
     3901 cgccaccggc tggtcgccca gcccggaggc tggcggctgg aggcgctgga ggccggcaag
     3961 ctcgtcgccc gcttcggcaa ccgccccgag cgcatggacc tcgaccaggg cgactacgtg
     4021 ctgtccagcg gcgacgattt ctacgtgtcg ctgcagcgcc gcaaggacgg caggcccgag
     4081 ggcccgcagc gtgaccgcgc cggcgaccgg cccggcgagc ccggcgtgga acgtccgctg
     4141 cgagacggtg cggcggcggg cagcgagccg gaggtcgggt gacatggggc tccaggaggt
     4201 gctgcagggc gtgctcgacg ggctcgccat ccaattgacc ggctccgaca cgccccagcc
     4261 cggggcgctc gtgggcgcca agatcgtccc gccgctcggc gagctcgacg tgaccgacgt
     4321 ggtggagggc gacgtcagcc tgacctggct caccaaggac gtgctgttcg gcaacgagga
     4381 gctgctcggg gcgccgtccg tgtccgcgat caactcggcg agcaccggcg gccgcccgga
     4441 ggtcaccgac ctcggcagcc tcgtcgccct gcccggcgtg cccgggctgg tgggtcagct
     4501 tgccggcacg ctcccgctgt cgtcgggggc caccgtcccg gtgcaggcga ccatcacgtg
     4561 gcaggtcctc gacgaggacg gccggacgga actgccgccg tcggagtacc gcatccccgg
     4621 cggcgccaac tcgcccacgg tgcaggtcgc gttcgcgccc ggcacgcacg agctgaccgg
     4681 cagcggcacc atcccggcgc cgcggcggcg ccacctcagc gcgacggtga cgctgaccgc
     4741 caacggcatc ccggcggggc ccaggacgct gccgcccgtg ccggtgctgg tgccgtcgct
     4801 gcccatcccg accgtgctgg cgatgttcct gcacacgaac ttccaggcgc gatccggtga
     4861 cgacgacggc gccgtcctcg tggtcgtgcc ggccagttcg ccgctgcgca gcgtccagca
     4921 gctcaccagc acgctgtcca ccctccaggg cgtgatcggc aacctcacgg cgttcggctc
     4981 gttcgccgcg ttcgcgctcg gcctcagcga gctggtcggc gccctgtccg cgcaaccgca
     5041 cgtgcagttc cgcgccgccg acagcatcgg caatctcaac gacatcacgc tgatccagcg
     5101 gtcctggtac gagaacgaca ccgaggcgga ggacgaactg agctcgctga tcctgatcgg
     5161 acgcgagggc cggggcgtgc ggtgctccaa cgcccgcggc caggacgacg acgaggggca
     5221 gttcaccgtc cggaccggat ccagcctgct ggccatcatc cgcagcctgc acagcgcgag
     5281 cccggcggcc gagggcggga cgatcgtcgt ggaccgcgcg cctccgggcg gctggtggaa
     5341 cccggaccag ttcggcgacg agctgtcctc catcacgttc atgtcgtgac cggcggccgc
     5401 ggccggttga cggtggtggt gcccgcgcca tggtgcgacc tggcggtgtg gcgagtccgt
     5461 gccggccagg ccgccggagg cgtgaacgag cccatcgagg tgccggcggg ccccacgcgg
     5521 gtcgtcatcg acgccgacgt gacgctgccg gccgtgcgcg cgggcgccgc cctgcggctg
     5581 tccgtcgacg tgccgtccgg cggcgctgtc cgcctgcacc ctccgctgtt gccctatcgt
     5641 gcgctgcctg acgcagacgg tggctgagcg ggaggagcaa ggaccatggt ggaaggcact
     5701 ggggcgcagg gcccccgggc ggcgctcgtg acgggcggtt ccagcggcat cggcctcgcg
     5761 ctggcgcgca tgctgggcga ggagggctac gcgctgacgg tgtcggcccg ccgggaggac
     5821 aagctcaagg cggcggccga ggagctgcgc gccgagggcc tggaggtcat cgaggtggcg
     5881 gccaacctgg gtgaggagga cgaggtccag cgcgtcgtcg cggcgcaccg cgagcgctac
     5941 ggccgcctgg acgtcctggt gaacaacgcc ggggtgggca tcggcgcgaa cgtcgcggag
     6001 atctccacca agaagatgga cctgcagctc aacctgaacc tgcgctcggc gatcctgttc
     6061 taccgcgact gcatggacat gctgcgggcg tccgcggccg agcaccgccg cacgcaggtg
     6121 tggaacaccg cgtcgatcgc cggcaagcgc ggcgaggcct ggctgtcggt gtactcggcc
     6181 accaaggcgg gactggtggg attcacccag gcgatgaaca aggagttggc cggtgacggc
     6241 atccgctcca cggcgctgtg cccggccttc gtggacacgc cgatgacgga cttcgtgaag
     6301 ggcgccgtgg cggccgacga catgatccgg ccggaggaca tcgcggagct gtgccgggcg
     6361 ctgctgcggc tgtcgcccgc cgcggtgatc ccggagatca tggtggtacg ccccggcgac
     6421 cagatgtagg ggtcactgca cgtagcgctc caccagctcc tccgggcgcg cctcggcctc
     6481 gccggggtcc tggcccgcgc cgcgcttggc ccgccgctgc cgcagcaggt cccagcactg
     6541 gtcgaggtcg cgctccagcg ccgccaggcg ctcccggtcc tttgggtcca gcgcggcctc
     6601 gccctgctcg cgcagtgcct cctcgcgctc gaccagctca tggatgcggc tcaggatctc
     6661 gcggtcgtcc atgggggcga cccttcccgg tggccttcgc cgtcatgccg gcgtgaggtt
     6721 gagattccag gcgcggggca tcctgcccgc ggcatatggc gatccatggg cgggccgaga
     6781 tctcggacgt cgcgaccctc gcgcgcgacg tggcatccgc gcgcgaccag ttgcaggcgc
     6841 tgctggaggg ccgctggccc ggtgacgtgg acctcgcgct catcgcggtc cacgaggcgc
     6901 tgatcaacgc cgaccgccac ggcgccggca tccgccacgc cacggcgcgg ctggaaggcg
     6961 accgggtcgt cgtggagatc gccgactacg gcgccggctt cgaccaccgc ggctacgtgg
     7021 ccagccagcc cgacctgctg gccgagcgcg gccgcggcct gtgggtgatc catcagatcg
     7081 cgtgccagtt cgagctcgtc cacgaggccg agggcagccg cgtcgtcatg gtgttcacgc
     7141 ccggcacgtt gggggtccag gcgccggagc cggaccgccc cccgctgccg gacgtcgacg
     7201 ccgtggccca gagcctcatc cgcgcgttcg gcgcggccgt ggccatcgtg gaccgcaacc
     7261 tggtgatccg ggccgcatgg ggtgacgtgg agggactgct ggggctcacg atggaggacg
     7321 tgctggagcg tgacgcccgc ggcctcacgg cggagctgaa gacccggttc caggacccgc
     7381 agcgctacga gcagcgcatc ctggacgtct acgcgcagcc ggaccaggtg tccgaggacg
     7441 tgttcgtccg gcgggacggg cgcatcgtgc gccgccacag cgtgccgttc acgtcccggg
     7501 gccagcactg gcggctgatc agctacatcc ccgtggccga gcagacccag gtcgtcgccg
     7561 ccgtgcagcg ctcgctgctg ccggacctgc cggagtggga cggcctggag gtcgccgcga
     7621 tctaccaccc ggccgaggcg agctccttcg tcggcgggga cttctacgac ttcatccagc
     7681 tcacctccgg cgcgcgctgc gtggtggtcg gcgacgtctc cgggcgcggg ccggcggccg
     7741 cggcgaccag cacgcacgtg cgcgcctacc tgcgcgccgc cctgcgcagc cacggcgtga
     7801 ccgccgccgt ggcggatctg gacgccacgc tggagcacga gctgccggcc gagcacttcg
     7861 tgaccctggc catgtgcacc caggagtccg ccggcgtgtg gacgcacacc aactgcggcc
     7921 acgttccggc tttgctgctg cgcgacggcg cggtcaagga gctcggcggc cgcggcggcg
     7981 cgctgggcat cgccctgccc gccgagtggc cgcgcgagcc gttcgtcctg cagccggacg
     8041 acgtgctgct gctgtacacg gacgggatcg tcgacgccgg gcggcgcgag cgccgctacg
     8101 gcgccgagcg gctcgccgac tccctgcggg agctgggcgg gctgccgccc cgggagctgg
     8161 tccgggcgct cgacgagcgc gtgcactcct tcgccgcgaa cccgctcgcc gacgaccatg
     8221 tcctcgtcgc ggtgcgccgc acgcggcccc ggcccgagct ggacgaccag cgggaggagc
     8281 cgacatgacg gctgtcgact ggcagggccg gctgggccgc gtcctggaga cggcgcgggc
     8341 acacgccgag gacgtggacc gccgggcgcg cttcccgcgc gaggtcgtcg acgcgctcgg
     8401 cgacgcgggc ctgctcgggc tcaccgtgcc ggaggaactc ggtgggctcg gcggctcgtt
     8461 cgcggaggcg tacgaggtgc tcgtgcgcct ggccgagcgc tgcgcgtcca gcgggatgat
     8521 cctgaccatg cactacgcgg cgctcgcggt gctgcgcgag gccggcgggg gacggttcga
     8581 cgcggtcctg cgcgaggcgt ccgccggccg gcacctgtcc acgctggcgc tgtcggagaa
     8641 ggccacgcgc agcagcttct ggatctccat gggcgaggcc aagcgcctgc cggacggcgc
     8701 cacgcggatc acggtcgaca agtcgttcgt caccagcgcc gggccggcga cgagctacgt
     8761 ggtctccacg ggcagcgcgg agcgcgccca tctgggggag agcgacctgt ggctggtcag
     8821 cgccgggcag cagggtgtgg aggtcctgga ctggtggcag ggttcgggcc tgcgcggcaa
     8881 ctccagcgcg ccgatgcgct tcgactgcga gctgccgccg gagtgcgccg tgtgcgaacc
     8941 gggctccggc gtggacgtca tcctgggcac cgtggtcccg tggttccagc tcggcgcctg
     9001 cgcggtctcc gtcggtgtgg cccgcgcggc ggtggagttc gcccgcgcgc acgtcaccgg
     9061 cacggtcctg gagcacctcg gccagcgcct ggtcgaccag ccggtggtcc ggcacacgct
     9121 cggccggctc ttcgcgcggg tcgactccgt ggacggcttc ggccgcgcca cggcccgcga
     9181 cctgtccgac gggcgcgcca ccatgaccca ggtcctggag
//
LOCUS       DATMKC010000264         4219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_219715, whole genome shotgun sequence.
ACCESSION   DATMKC010000264 DATMKC010000000
VERSION     DATMKC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219715"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VNU01_07675"
     CDS             complement(<1..311)
                     /locus_tag="VNU01_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32536.1"
                     /translation="MDGPVVALDAMGGDHAPDAVVAGALRAHAEHGIAVVLVGRPDEV
                     GDALRRQGSPGGLTVVAAEDVVAMDEEPALALRAKPRASVHVAADLVAAGEAANVAPV
                     DP"
     gene            complement(319..501)
                     /gene="rpmF"
                     /locus_tag="VNU01_07680"
     CDS             complement(319..501)
                     /gene="rpmF"
                     /locus_tag="VNU01_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWH32537.1"
                     /translation="MAVPKRKKSRSRTRSRKAQWLRAALPARASCARCKSPIRPHTAC
                     GTCGYYAGRQVVQIED"
     gene            complement(522..1097)
                     /locus_tag="VNU01_07685"
     CDS             complement(522..1097)
                     /locus_tag="VNU01_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWH32538.1"
                     /translation="MTASRSDRVLSVADLVGRPGASRRLALEVVPPDDLDRPLLRLEQ
                     PVRLEGVLESVVDGILVRGTLSTSVEAQCGRCLKDIVADVETDVVELFRDPAEVDPDD
                     ELDAGYEIADARIDLDVLVRDALAPALPYSPVCDEDCKGLCPTCGTDLNEASCDCTDT
                     STDARWAALEALRLPPTGGDGEDVERGDGGA"
     gene            complement(1263..1844)
                     /locus_tag="VNU01_07690"
     CDS             complement(1263..1844)
                     /locus_tag="VNU01_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32539.1"
                     /translation="MDVESHISHLEQLIAEARPVPLSASVMVNRREIDELLAALRAAM
                     PEEMRQARWVLKERDELLAQGQREAEQLVADAQAEQQRLVSDTEVVRASRREAERILE
                     DAREQARILRLEAEDYVDGKLANFEIALQKTLRTVEKGRERLRGRLASDELAESAAAA
                     VEEDDDTGEWGALTPQPPAQQFYDHEASGSPGE"
     gene            complement(1863..2348)
                     /gene="coaD"
                     /locus_tag="VNU01_07695"
     CDS             complement(1863..2348)
                     /gene="coaD"
                     /locus_tag="VNU01_07695"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWH32540.1"
                     /translation="MSVTAVCPGSFDPVTYGHLDVLERAASRFDRLVVAVLRNASKSP
                     LFDIDERMELLREVTAPWGNVEVESFEGLLVDFCRDRGVRLIVKGLRAVSDFEYELQM
                     AQMNSRLGDVETLFLTTSPVYSFLSSSLVKEVARFGGDVSDFVPPVVAERLRERLSAP
                     R"
     gene            complement(2345..2926)
                     /gene="rsmD"
                     /locus_tag="VNU01_07700"
     CDS             complement(2345..2926)
                     /gene="rsmD"
                     /locus_tag="VNU01_07700"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWH32541.1"
                     /translation="MRVIAGSARGRRLVAPSGPGTRPTADRVKEALFSSLGELRDAVV
                     LDLYAGSGALAIEALSRGAARAVLVEQDRRALEAIERNLAATGLAGAVEVVRADVARY
                     CREPRGGPFDLVLVDAPYDEPQQALFACLADLAAAGALPEDALVVVERDRRADDPDPP
                     GWLARERARSYGDTVLRYFRVSGPLPAGRQEDS"
     gene            complement(3009..>4219)
                     /locus_tag="VNU01_07705"
     CDS             complement(3009..>4219)
                     /locus_tag="VNU01_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWH32542.1"
                     /translation="LVAAWAMLCALDAGRQAVLMAPTEVLAEQHVRTLSALLAPLGVN
                     VLDGPRLELLTGGTPAKKLRGLLAELASGDVQLLVGTHALLEDRVLFGDLGVVVVDEQ
                     HRFGVEHRARLRDKRSDGKVPDVLVMTATPIPRSLALTLYGDLDVTVLDELPPGRKPV
                     VTQVIASDSPRRQTLYGFIRERVAAGERAYVVCPLVEDSEALAAVASAEAMHARLSTE
                     VFPDLNVGLVHGRISTAERDAVMEAFRKGEVQILVATTVIEVGVDVPEATVMVIEDAD
                     RFGLSQLHQLRGRVGRGADASYCVLFSAEPDGNPRLEALAATGDGFLLAETDLELRGE
                     GSLFDTRQSGLPDLKLAQLIRDFDWVVRTREDARELVERDPELAEHPELLAEVRRRYG
                     DERLAALEVG"
BASE COUNT          566 a         1619 c         1496 g          538 t
ORIGIN      
        1 gggtccaccg gcgccacgtt ggccgcctcc ccggcggcga caaggtccgc ggcgacgtgc
       61 acgctggcgc gcggcttcgc gcgtagcgcc agcgcgggct cctcatccat cgccacgacg
      121 tcctcggccg ccacgaccgt cagtccgccg gggctgccct ggcggcgcag cgcgtcgccc
      181 acctcgtcgg ggcggccgac gagcaccacc gcgatgccgt gctcggcatg ggcgcgcagc
      241 gcgccggcca cgacggcatc cggcgcatgg tcgccgccca tcgcgtcgag cgcgacgacc
      301 ggcccgtcca cgggccggtc agtcctcgat ctggacgacc tggcggccgg cgtagtagcc
      361 gcacgtgccg cacgcggtgt gcgggcggat cggcgacttg cagcgggcgc aggacgcgcg
      421 cgccggcagg gcggcgcgca gccactgggc cttgcgggac cgcgtgcggg accgcgactt
      481 cttgcgcttg ggaacagcca ctttcgaacg ctcctgtcag atcaagcgcc gccgtcgccc
      541 cgctcgacgt cctcaccgtc gccgccggtt ggcggcaggc gaagcgcctc gagcgcggcc
      601 cagcgggcgt cggtgctcgt gtcagtgcag tcgcaggacg cctcgttgag gtcggtcccg
      661 caggtggggc agaggccctt gcagtcctcg tcgcacacag gactgtacgg cagcgcgggc
      721 gcgagcgcgt cccgcaccag cacgtccagg tcgatgcgcg cgtcggcgat ctcgtagccg
      781 gcgtccagct cgtcgtccgg gtcgacctcc gccgggtccc ggaacagctc cacgacgtcc
      841 gtctcgacgt cggcgacgat gtccttcagg cagcgcccgc actgggcctc gacgctggtg
      901 gacagcgtgc cccgcaccag gatcccgtcg accacgctct ccaggacgcc ctccagccgg
      961 accggctgct ccaggcgcag cagcggccgg tcgaggtcgt ccggcggcac cacctccagc
     1021 gccaggcggc gcgacgcgcc agggcggccg acgaggtcgg cgaccgagag gacgcgatca
     1081 ctgcgagagg cggtcatggc ggggaaaacg gctccagcgg ccgacagagt cagcccaacg
     1141 ggtcacggac gggtgcgggc acggcgaatc ccggccgcgg acagctggga tcgaccgtcc
     1201 aaggtagcag caggcgccgc ggcgggcaca cgcgcggccg ggcgctcagc tcaggcttgt
     1261 gctcactccc ccgggctccc cgaggcctcg tggtcgtaga actgctgggc cggcggctgg
     1321 ggcgtcagcg cgccccactc gccggtgtcg tcgtcctcct cgacggccgc ggcggccgac
     1381 tcggccagct cgtcgctggc caggcggccg cgcagccgct cgcggccctt ctccacggtc
     1441 cgcagcgtct tctgcagcgc gatctcgaag ttggcgagct tgccgtcgac gtagtcctcc
     1501 gcctccaggc gcaggatccg ggcctgttcc cgggcgtcct caaggatgcg ctcggcctcg
     1561 cggcgggagg cgcgcaccac ctcggtgtcg ctgaccagcc gctgctgctc cgcctgcgcg
     1621 tcggcgacca gctgctccgc ctcgcgctgg ccctgggcca gcagttcgtc gcgctccttg
     1681 agcacccagc gggcctggcg catctcctcg ggcatggccg cccgcagggc cgccaggagc
     1741 tcgtcgatct ccctgcggtt gaccatcacc gacgccgaca gcggcacggg gcgcgcctcg
     1801 gcgatcagct gctccagatg cgagatgtgg gactcgacgt ccacgggcgt gggtcctttg
     1861 gtctaccggg gagcggaaag tcgctctctg agccgttccg ccaccacggg cggcacgaag
     1921 tcggacacgt cgccgccgaa gcgggcgacc tccttcacca gcgacgagga gaggaaggag
     1981 tatacggggc tggtggtcag gaacagggtc tcgacgtcgc ccaggcgcga gttcatctgc
     2041 gccatctgca actcgtactc gaagtcgctg accgcccgca gccccttcac gatgagccgc
     2101 accccccggt cgcggcagaa gtcgacgagc agcccctcga agctctccac ctccacgttg
     2161 ccccagggcg ccgtgacctc gcgcagcagc tccatgcgct cgtcgatgtc gaacagcggg
     2221 ctcttgctcg cgttgcgcag caccgccacg accagccggt cgaagcgcga ggcggcacgc
     2281 tccaggacgt ccaggtgacc gtaggtcacc gggtcgaagc tgccgggaca gaccgcggtg
     2341 acgctcacga gtcctcctgt cgcccggcag ggagcggtcc gctcacccgg aaatagcgca
     2401 gcacggtgtc accgtacgag cgcgcgcgct cccgcgccag ccagccgggc gggtccggat
     2461 cgtcggcgcg ccggtcgcgc tcgaccacca cgagcgcgtc ctcgggcagc gctccggccg
     2521 cggcgaggtc ggccaggcac gcgaacagcg cctgctgcgg ctcgtcgtag ggcgcgtcga
     2581 cgagcacgag gtcgaagggg ccgccgcgcg gctcccggca gtagcgggcc acatcggcgc
     2641 gcaccacctc cacggcgccg gcaaggcccg tggcggcgag gttgcgctcg atcgcctcca
     2701 gcgcgcggcg gtcctgctcc accagcacgg cgcgcgcggc gccgcgcgac agcgcctcga
     2761 tcgccagcgc gcccgagccc gcgtacaggt ccagcacgac ggcgtcgcgc agctcgccga
     2821 gcgagctgaa cagcgcctcc ttgacgcggt cggcggtggg ccgggtgccg ggccccgacg
     2881 gcgcgaccag gcgccggccc cgggcgctgc ccgcgatcac ccgcacgtat ccacagcttt
     2941 cggcctcgag cgggtgcact tccggagcga tggctctgga agtgcaccca acggtgggag
     3001 cccgggcatc agccgacctc cagtgcggcg aggcgctcgt cgccgtagcg gcggcgcacc
     3061 tccgccagca gttccgggtg ctcggcgagc tccgggtcgc gctcgacgag ctcgcgcgcg
     3121 tcctcgcgcg tgcgcaccac ccagtcgaag tcgcggatga gctgggcgag cttgaggtcc
     3181 ggcagcccgg actggcgcgt gtcgaacagg ctgccctcgc cgcgcagctc caggtccgtc
     3241 tcggcgagca ggaagccgtc gcccgtggcc gccagcgcct ccagccgcgg gttgccgtcc
     3301 ggctcggcgc tgaacagcac gcagtagctc gcgtccgcgc cgcgtccgac gcggccgcgc
     3361 agctggtgca gctgcgacag gccgaagcgg tcggcgtcct cgatcaccat caccgtcgcc
     3421 tcgggcacgt ccacgccgac ctcgatcacg gtggtcgcga ccaggatctg cacctcgccc
     3481 ttgcggaacg cctccatgac cgcgtcgcgc tcggcggtgg agatgcggcc gtgcaccagg
     3541 ccgacgttca ggtcgggaaa gacctccgtg gacagccggg cgtgcatcgc ctcggcgctg
     3601 gccacggcgg ccagcgcctc gctgtcctcc accagcgggc acaccacgta cgcgcgctcc
     3661 cccgccgcga cgcgctcccg gatgaagccg tacagcgtct ggcgccgggg cgagtcgctg
     3721 gcgatgacct gcgtcaccac gggcttgcgc cccggcggca gctcgtccag gaccgtgacg
     3781 tccaggtcgc cgtacagcgt cagcgccagg gagcgcggga tcggcgtggc cgtcatcacc
     3841 agcacgtcgg gcaccttgcc gtcgctgcgc ttgtcgcgca ggcgcgcgcg gtgctccacg
     3901 ccgaagcggt gctgctcgtc gaccaccacc acgcccaggt caccgaacag cacgcggtcc
     3961 tccagcagcg cgtgcgtgcc gaccaggagc tgcacgtcac cggaagcgag ctcggccaag
     4021 agcccccgga gcttcttcgc gggcgtgccg ccggtgagca gctccaggcg cggcccgtcc
     4081 agcacgttga cgcccagcgg cgccagcagc gccgacagcg tgcgcacgtg ctgctcggcc
     4141 aggacctcgg tcggcgccat gagcacggcc tgccggccgg cgtccagcgc gcacagcatc
     4201 gcccaggccg ccaccagcg
//
LOCUS       DATMKC010000265         4277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_313155, whole genome shotgun sequence.
ACCESSION   DATMKC010000265 DATMKC010000000
VERSION     DATMKC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313155"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VNU01_07710"
     CDS             complement(<1..462)
                     /locus_tag="VNU01_07710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWH32543.1"
                     /translation="MRLWDGASGPHFFVEPGDVFGDEAVLRGEQARHLAVVLRARRGD
                     PVSLADGTGARFRAVVDTAAPDEVRLRLLEQEMVRQPRPAITVVHALPKGRKLDDVVE
                     KLSEVGADALVPVHSARSVVRLDAAKADKARARWEAVALAAAKQSRRARPLR"
     gene            complement(480..1595)
                     /locus_tag="VNU01_07715"
     CDS             complement(480..1595)
                     /locus_tag="VNU01_07715"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWH32544.1"
                     /translation="MRDLYEILGVARDADAETIKRAYRRKARELHPDAGGDEESFKEL
                     TAAYEILSNERARANYDRYGDPRGPAGAEGFEGFSGFGDLQDFIEAFFGGFGGASRRQ
                     SARAAARGGRDALVDVRLTLDEAFTGVRHDVELTLPRLCEACEGSGAAPGGGPVRCEE
                     CGGAGAVQQVARSVFGQVLTTGTCPSCEGLGTRVLEPCPSCDGQGRREVTETITVDIP
                     PGVDDGTRLRLTGRGESGRLGGQSGDLYVRVRLAQHPIFTRDGEDLHCELRLPMSQAA
                     LGAELRVPTLAGEQPVQVPAGVQNGEVIALKGHGMPRLGQPRTRGRLHIHVRVITPTD
                     LTPEQEEVLRRFAALRNEEVREEGKRFFGRLREAFGA"
     gene            complement(1647..2714)
                     /gene="hrcA"
                     /locus_tag="VNU01_07720"
     CDS             complement(1647..2714)
                     /gene="hrcA"
                     /locus_tag="VNU01_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWH32545.1"
                     /translation="METKLDERKAAILKAIVQDYVREGEPVGSKRLVEGWGLGVSSAT
                     VRAEMAALEEAGYIAQPHTSAGRIPTDKGYRYFVDALAERTPLSEHQRQTLLDLLRDS
                     SDLEELLRRASTVLSRLTRYAALVVAPALDRSRLRHIELVQLGPTSVLVVFIADTGRV
                     DKRMLDLDAPVTEHDVARARHAVNEAAAGLRTSQTPDVVAGIATGAPPEVRPLLEAVA
                     DAVRRGLSSPPPGEQIIVGGTANMAAPGYFERLEQVKSVYETLEEQVIVLQMLQDALQ
                     VGDPAVRIGHELPLVELAACSVVAASYDAAGSSAGTVGVLGPTRMDYPRTLAVVQAVA
                     SSIEQALAGITGSSGAEPTRN"
     gene            complement(2816..3934)
                     /gene="hemW"
                     /locus_tag="VNU01_07725"
     CDS             complement(2816..3934)
                     /gene="hemW"
                     /locus_tag="VNU01_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWH32546.1"
                     /translation="MTTAPGIYLHVPFCASICHYCDFNRVLGDDPELMRRYADAMVRE
                     VERVLAARDDWPAFGSVFIGGGTPTLLRDQLCPIVDATRPALGDGAEVTVEANPETVT
                     AELMAQLREAGVNRVSMGAQSFAPHVLEFLGRRHSADKPLEAVEAVRAAGIERVSLDL
                     IYGAPGESDADWEHSLRTAVDAGLDHVSAYALTVEANTPYAAMVKADPRLAPDDDVQA
                     RRMDQAARILGEAGLARYEVSNWARPGQESRHNLVYWRGGDWLGIGAGAHGHWRGRRT
                     WQVRAPQRWVELVEAGQEPLGGEETLTPAQRREERLLMGLRIAEGVARADVEPIDEAA
                     AEALVAQGLLHDDGERIAVTERGRSLTGAIILRLLPPD"
     gene            complement(3984..>4277)
                     /locus_tag="VNU01_07730"
     CDS             complement(3984..>4277)
                     /locus_tag="VNU01_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWH32547.1"
                     /translation="VERVGAVTMALWGPGDVDLALANASVYLEAVGHVVVAWLWLEQY
                     LAAAGKDGEFYDGKRQATRYFYRWELPKTGPQFDLLESLDTTTLDMRDAWFSA"
BASE COUNT          522 a         1680 c         1501 g          574 t
ORIGIN      
        1 ccgcagcggc ctggcccgcc ggctctgctt ggcggccgcc agggcgaccg cctcccagcg
       61 ggcacgcgcc ttgtccgcct tggccgcgtc cagccgcacc acgctgcgcg cgctgtgcac
      121 gggtaccagc gcgtcggcgc ccacctccga cagcttctcc accacgtcgt cgagcttgcg
      181 gcccttcggc agggcgtgca cgaccgtgat cgccggcctg ggctgccgca ccatctcctg
      241 ctccagcagc cgcaggcgca cctcgtcggg cgccgcggtg tccacgacgg cgcggaagcg
      301 cgccccggtc ccgtccgcca ggctgaccgg gtcgccccgg cgggcgcgca ggaccaccgc
      361 gagatggcgc gcctgctcgc cgcgcaggac cgcctcgtct ccgaagacgt cacccggctc
      421 gacgaagaaa tggggcccgc tggcgccgtc ccacagcctc atcccgccgc ctggcgagct
      481 cacgcgccga acgcctcgcg caggcggccg aagaagcgct tgccctcctc gcgcacctcc
      541 tcgttgcgca gcgcggcgaa gcggcgcagc acctcctcct gttccggggt gagatcggtg
      601 ggcgtgatca cgcgcacgtg gatgtgcagc cgcccgcgcg tgcgcggctg tcccagccgc
      661 ggcatgccgt ggcccttgag ggcgatgacc tcgccgttct gcacgcccgc cggcacctgc
      721 accggctgct cgccggccag cgtgggcacg cgcagctcgg cgccgagcgc ggcctggctc
      781 atgggcaggc gcagctcgca gtgcaggtcc tccccgtcgc gcgtgaagat cgggtgctgc
      841 gccaggcgca cgcgcacgta caggtcgccc gactggccgc ccagccggcc ggactcgccg
      901 cggccggtca gccgcagccg cgtgccgtcg tccacgcccg gcgggatgtc gacggtgatg
      961 gtctcggtga cctcccgccg gccctgcccg tcgcaggagg ggcacggctc cagcacgcgc
     1021 gtgccgagcc cctcgcacga cgggcacgtg cccgtggtga ggacctggcc gaacacgctg
     1081 cgcgcaacct gctgcaccgc gcccgcgccg ccgcactcct cgcagcgcac ggggccgcct
     1141 ccgggcgcgg cgccgctgcc ctcgcacgcc tcgcacagcc gcggcagcgt cagctccacg
     1201 tcgtggcgca cgccggtgaa cgcctcgtcc agggtgagcc ggacgtccac cagcgcgtcg
     1261 cggccgccgc gcgccgccgc gcgcgcggac tgtcggcggg aggccccgcc gaagccgccg
     1321 aagaacgcct cgatgaagtc ctggaggtcg ccgaagccgg agaagccctc gaagccctcc
     1381 gccccggcgg gaccgcgcgg gtcgccgtag cggtcgtagt tcgcgcgcgc gcgctcgttc
     1441 gacaggatct cgtaggcggc ggtcagctcc ttgaaggact cctcgtcgcc gccggcgtcc
     1501 gggtggagtt cacgggcctt gcgccggtac gcgcgcttga tcgtttccgc atcggcgtcg
     1561 cgcgcaacgc cgaggatctc gtagagatcg cgcacttgcc tacctgacct gcccctgctc
     1621 cgcggggcgc cgccccggcg ggatgcctag ttcctggtgg gttccgcccc gctcgagccg
     1681 gtgatgcccg cgagcgcctg ctcgatcgac gaggctaccg cctgcacgac cgccagggtg
     1741 cgcgggtagt ccatgcgcgt ggggcccagc accccgacgg tgcccgcgga cgagccggcg
     1801 gcgtcgtagc tggcggccac cacggagcag gccgccaact ccaccagcgg cagctcgtgg
     1861 ccgatgcgca ccgccgggtc gcccacctgg agcgcgtcct gcagcatctg caggacgatg
     1921 acctgctcct ccagcgtctc gtagacggac ttcacctgct ccaggcgctc gaagtagccg
     1981 ggcgcggcca tgttggccgt gccgccgacg atgatctgct cccccggcgg cgggctggac
     2041 aggccccggc gcaccgcgtc ggcgaccgcc tccagcagcg ggcgcacctc cggcggcgcg
     2101 ccggtggcga tcccggccac cacgtccggg gtctggctgg tgcgcagccc ggcggcggcc
     2161 tcgttgacgg cgtggcgcgc acgcgcgacg tcgtgctccg tgaccggcgc gtccaggtcc
     2221 agcatgcgct tgtcgacccg gccggtgtcg gcgatgaaga cgaccagcac gctggtgggg
     2281 ccgagctgca ccagctcgat gtggcgcagg cggctgcggt ccagcgcggg cgcgaccacc
     2341 agcgcggcat agcgcgtcag gcgcgacagc accgtggagg cgcgccgcag cagctcctcc
     2401 aggtcactgg agtcccgcag caggtccagc agggtctggc gctggtgctc gctcagcggg
     2461 gtgcgctcgg ccagcgcgtc gacgaagtag cggtagccct tgtcggtggg gatgcggccc
     2521 gcggaggtgt gcggctgcgc gatgtagccg gcctcttcca gcgcggccat ctcggcgcgc
     2581 acggtggcgg aggacacccc gagcccccag ccctccacca ggcgcttgga gccgaccggc
     2641 tcgccctcgc gcacgtagtc ctgcacgatg gccttgagga tcgcggcctt gcgctcgtcc
     2701 agcttcgtct ccacgcctgc cgctcacctc ctgccgtgtc gcctcggctc cgatgctggc
     2761 aatcatgcac cgggagtgcc agcccatcat gacattcccc ggatcccccg ctcaatcagt
     2821 ccggcggcag caggcgcagg atgatcgcgc ccgtgagcga gcggccccgc tccgtcacgg
     2881 cgatgcgctc gccgtcgtcg tgcagcagtc cctgggcgac cagcgcctcg gcggcggcct
     2941 cgtcgatggg ctccacgtcc gcgcgggcca ccccctcggc gatgcgcagc cccatgagca
     3001 gccgctcctc gcggcgctgc gcgggcgtga gcgtctcctc gcccccgagt ggttcctggc
     3061 ccgcctcgac cagttccacc cagcgctgcg gcgcgcgcac ctgccaggtg cgacgccccc
     3121 gccagtgccc gtgcgcgccc gcgccgatgc cgagccagtc cccgccgcgc cagtacacga
     3181 ggttgtggcg cgactcctgc cccggccgcg cccagttgct gacctcgtag cgggccagcc
     3241 ccgcttctcc gaggatccgc gcggcctggt ccatgcggcg cgcctggacg tcgtcgtccg
     3301 gggccaggcg cgggtcggcc ttcaccatgg ccgcgtacgg cgtgttggcc tcgacggtga
     3361 gcgcgtaggc cgagacgtgg tcgagcccgg cgtccaccgc ggtccgcagg ctgtgctccc
     3421 agtccgcgtc ggactctccg ggcgccccgt agatgaggtc caggctgacc cgctcgatgc
     3481 ccgccgcgcg cacggcctcg accgcctcca ggggcttgtc cgcactgtgg cggcggccca
     3541 ggaactcaag gacgtgcggc gcgaagctct gcgcgcccat gctgacccgg ttgacgcccg
     3601 cctcgcgcaa ctgggccatg agctcggcgg tcaccgtctc cgggttggcc tcgaccgtga
     3661 cctccgcgcc gtcccccagt gcgggacgag tcgcgtccac tatcggacac aactggtccc
     3721 gcagcagggt gggggtgccg ccgccgatga acacgctgcc gaacgccggc cagtcgtccc
     3781 gggcggccag cacccgttcc acctcgcgca ccatggcgtc ggcgtagcgc cgcatcagct
     3841 ccgggtcgtc gccgagcacg cggttgaagt cgcagtagtg gcagatcgac gcgcagaacg
     3901 gcacgtgcag gtagatgccc ggggcggtgg tcacccggcc aggatggcac gccgtcccgg
     3961 acccgccgag cgaggcggcc cggtcaggcg gagaaccagg cgtcgcgcat gtcgagggtg
     4021 gtggtgtcca gcgactccag caggtcgaac tgcggcccgg tcttcggcag ctcccagcgg
     4081 tagaagtacc gggtcgcctg gcgtttgccg tcgtagaact cgccgtcctt gccggccgcg
     4141 gccaggtact gctccagcca cagccacgcg accaccacgt ggccgaccgc ctccaggtac
     4201 accgaggcgt tcgccagcgc caggtcgacg tcgcccggcc cccacagcgc catggtgacc
     4261 gcgccgacgc gctccac
//
LOCUS       DATMKC010000266         4824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_145608, whole genome shotgun sequence.
ACCESSION   DATMKC010000266 DATMKC010000000
VERSION     DATMKC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145608"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VNU01_07735"
     CDS             complement(<1..296)
                     /locus_tag="VNU01_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWH32548.1"
                     /translation="MSARLVLATRNEGKVAELRRILDGLDVELLSARDVDLPDVEETG
                     ETFEDNALLKARESARACGLPCVSDDSGLVVDALGGEPGVRSARYAGPHGDDEA"
     gene            complement(293..1033)
                     /gene="rph"
                     /locus_tag="VNU01_07740"
     CDS             complement(293..1033)
                     /gene="rph"
                     /locus_tag="VNU01_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWH32549.1"
                     /translation="MTAAPTTARIDGRAPDQLRPVRMELGVQEYAEGSVLVHFGKTRV
                     LCAASVEESVPRWMKGSGKGWVTAEYAMLPRATDSRNDRERGKIGGRTQEIQRLIGRS
                     LRSVVDLKGMGEQSILVDCDVVQADGGTRTAAITGAWVALSLALDALVARKLVARRPR
                     LDPLCAVSVGIVGGVSLLDLCYAEDAGAEVDMNVVMTGEGRFVELQGTAEGLPFTRGQ
                     MDELVDLALAGGEQLFAMQRAVLEAAAS"
     gene            complement(1030..1779)
                     /locus_tag="VNU01_07745"
     CDS             complement(1030..1779)
                     /locus_tag="VNU01_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWH32550.1"
                     /translation="MRLTVMGCAGTHPAADRACSSYLVEHDGYRLLVDCGNGALSNVQ
                     KTLDPADIDAVLLSHLHVDHFGDLYGLYYALRFHGAGPRSVPVHAPPGADRHIAQLLH
                     GDPDDSFHAVCRFSEARPGDTLELGPLRVELHAANHPITTLAPRISAAGAVLAYTADS
                     GPADTLVDCARDADLLLADCSWLERMRPLPEGVHMTAREAGELAQRAGAARLVVTHVY
                     PANDLAEAAAEAAEAYGGEILIARDLLELDL"
     gene            complement(1776..2669)
                     /gene="murI"
                     /locus_tag="VNU01_07750"
     CDS             complement(1776..2669)
                     /gene="murI"
                     /locus_tag="VNU01_07750"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWH32551.1"
                     /translation="MSAPSSPPPAGDPRPIGVFDSGVGGLTVARALMDLLPEERVVYY
                     GDTARGPYGPRDLAEVRGFTAEIVGWLAAQDTKLVVAACNTATAAALDPAQNVGLEPP
                     DFPVPVVGVIAPAVRAAVAATRNGRVGVIGTVGTIGSGAYEHAVAQLAPDIDVVSQAC
                     PRFVELSEAGRTTDPDVLAVAREYLAPLQAAGVDTVILGCTHYPLLTGVLSYVLGPGV
                     ALISSAEETARAVFGTLIDDGLLAHRHGDSSPPEHRFVASGDPAAFARLAARFLGPRL
                     SEPDVERAEPLRAPQPSGTGG"
     gene            complement(2700..3644)
                     /locus_tag="VNU01_07755"
     CDS             complement(2700..3644)
                     /locus_tag="VNU01_07755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWH32552.1"
                     /translation="MLADDVSQAVGDTPLVRLPNLSPDGYEIYLKLEGHNPTGSVKDR
                     VAKYLLEQAERDGKLTPGMRILEPTSGNTGIALAALCAPKGYALTCVMPENVSDERRQ
                     LLGMYGVELVFSPANEGSNGSVRVAREMAEADPGWFMPFQYGNPANPQAHYETTAPEI
                     LRDLPDVAAFVGGLGTGGTLTGVGRRLKEHDPAIKVFAAEPEYGDLVYGLRNIDEGFI
                     PPVLDQTVLDSRIKVNSLKALGATRDLVRREGIFAGVSTGAALHVAVRVCESGRVPPG
                     GKVVVLSPDGGWKYLSTGAYAPGDLEAIAETLQNTLWA"
     gene            complement(3649..3930)
                     /locus_tag="VNU01_07760"
     CDS             complement(3649..3930)
                     /locus_tag="VNU01_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWH32553.1"
                     /translation="MAIEVRIPTVLREHTLGEKKVTGQGATLRELVADLESRYPGIGP
                     RLVGEDGQLHRFINVYVDDEDVRYLGGVETPLRDGAEVSILPAVAGGAF"
     gene            complement(3976..4419)
                     /locus_tag="VNU01_07765"
     CDS             complement(3976..4419)
                     /locus_tag="VNU01_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWH32554.1"
                     /translation="MTKAAIEGTVDLSRQVYDQMVAHARSDFPYEVCGMVAGTGGRFD
                     RHYEIPNAERSMTFYKMDGKAHLKAITEIEDNDWEFGIYHSHTHTEAFPSVTDVELAV
                     YPEAVYLIVSLQDPEPVIRAFDIRDGRITERVLTVDGEQAPAGNR"
     gene            complement(4446..>4824)
                     /locus_tag="VNU01_07770"
     CDS             complement(4446..>4824)
                     /locus_tag="VNU01_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWH32555.1"
                     /translation="ATGATDVTGFGMLGHLRKMAAASRVDLDLDTAAVPLLPHARELA
                     AQGVVPGGTRRNHEWVAEFTDAGATDETTRLLLSDAQTSGGLLFGAAPDRAAEAVARL
                     RGSGHDAAVIGEARPGSGTLHLH"
BASE COUNT          611 a         1739 c         1799 g          675 t
ORIGIN      
        1 ttcgcctcgt cgtcgccgtg cgggccggcg tagcgagcgg agcgcacgcc gggctcgccg
       61 ccgagcgcgt ccaccacgag gccggagtcg tccgacacgc acggcagccc gcacgcccgc
      121 gcggactcgc gcgccttgag cagcgcgttg tcctcgaacg tctcgccggt ctcctccacg
      181 tcgggcaggt cgacgtcgcg cgccgacagc agctccacgt ccaggccgtc caggatccgg
      241 cgcagctcgg cgaccttgcc ctcgttccgg gtcgcgagca ccagccgcgc gctcacgacg
      301 ccgcggcctc cagcacggcc cgctgcatgg cgaagagctg ctcgcccccg gccagcgcga
      361 ggtccaccag ctcgtccatc tggcctcgcg tgaacggcag gccctcggcg gtgccctgca
      421 gctccacgaa gcgcccctcg ccggtcatga cgacgttcat gtcgacctcg gcgcctgcgt
      481 cctccgcgta gcacaggtcc agcagggaca ccccgcccac gatcccgacg ctcaccgcgc
      541 acagcgggtc caggcgcggc cgacgcgcga ccagcttgcg cgcgaccagc gcgtccagcg
      601 ccagggacag cgcgacccac gcgccggtga tcgccgccgt gcgcgtgccg ccgtcggcct
      661 ggacgacgtc gcagtccacc aggatgctct gctcgcccat gcccttcagg tccaccacgg
      721 agcgcaggga ccggccgatg agccgctgga tctcctgcgt ccgcccgccg atcttgccgc
      781 gctcgcggtc gttgcgggag tcggtggcgc gcgggagcat cgcgtactcg gcggtgaccc
      841 agcccttgcc ggagcccttc atccagcgcg gcacgctctc ctcgacgctg gcggcgcaca
      901 gcacccgggt cttgccgaag tgcaccagca cactgccttc ggcgtactcc tgcacgccga
      961 gctccatgcg gaccggtcgg agctggtccg gggcgcggcc gtcgatgcgg gcggtggtcg
     1021 gggcggctgt cacaggtcca actccagcag gtcgcgggcg atcaggatct cgccgccgta
     1081 ggcctccgcg gcctccgcgg cggcctcggc caggtcgttg gccgggtaga cgtgggtgac
     1141 cacgaggcgc gcggcgccgg cgcgctgggc cagctcgccc gcttcccgcg cggtcatgtg
     1201 cacgccttcc ggcagcgggc gcatgcgctc cagccacgag cagtcggcca gcagcaggtc
     1261 cgcgtcgcgc gcgcagtcca ccagggtgtc ggccggaccg ctgtcggcgg tgtaggccag
     1321 cacggcgccc gcggcgctga tgcgcggcgc cagcgtggtg atgggatggt tggcggcgtg
     1381 cagctccacg cgcagggggc ccagctccag ggtgtcgccg gggcgcgcct cggagaagcg
     1441 gcacacggcg tggaagctgt cgtcggggtc gccgtgcagg agctgggcga tgtgccggtc
     1501 ggcgccgggc ggcgcgtgca ccggcacgga ccgcggcccg gcgccgtgga agcgcagcgc
     1561 gtagtacagc ccgtagaggt cgccgaagtg gtcgacgtgc agatggctga gcagcaccgc
     1621 gtcgatgtcg gcggggtcca gcgtcttctg cacgttggac agcgccccgt tgccgcagtc
     1681 gaccagcagc cggtagccgt cgtgctccac caggtaggac gagcacgcgc ggtcggccgc
     1741 cgggtgggtg cccgcgcacc ccatgaccgt gagcctcatc cgccggtccc cgagggctgc
     1801 ggggcgcgca acggttcggc gcgctcgacg tccggctcgg acagccgcgg gcccaggaag
     1861 cgggcggcca gccgcgcgaa cgcggcgggg tcgccgctgg cgacgaagcg gtgctcgggc
     1921 ggtgaggagt cgccgtggcg gtgcgccagg agcccgtcgt cgatcagcgt gccgaacacc
     1981 gcgcgggcgg tctcctcggc gctggagatc agcgccacgc cggggcccag cacgtagctc
     2041 agcacgccgg tgagcagcgg gtagtgggtg cagcccagga tgacggtgtc cacgcctgcg
     2101 gcctgcagcg gcgcgaggta ctcgcgcgcg acggccagga cgtcggggtc ggtggtgcgg
     2161 ccggcctcgc tgagctccac gaagcgcggg caggcctggc tgaccacgtc gatgtccggg
     2221 gcgagctgcg cgacggcgtg ctcgtaggcg ccggagccga tggtgccgac cgtgccgatg
     2281 acgccgacgc ggccgttgcg cgtggcggcc acggccgcgc ggaccgccgg cgcgatcacg
     2341 ccgacgaccg ggaccgggaa gtccggcggt tccagcccca cgttctgggc cgggtccagc
     2401 gccgcggcgg tggcggtgtt gcaggcggcg accaccagct tggtgtcctg ggcggccagc
     2461 catccgacga tctcggcggt gaagccgcgc acctcggcga ggtcacgggg gccgtagggc
     2521 cctcgcgccg tgtcgccgta gtagacgacg cgctcctccg gcagcaggtc catcagcgcc
     2581 ctggccacgg tgagcccgcc cacgccggag tcgaacacgc cgatggggcg cgggtcgccg
     2641 gcgggcggtg gcgaggaagg agcggacatg gcctgcatgc tagggggtgc ctgcccggat
     2701 caggcccaca gcgtgttctg gagcgtctcg gcgatggcct ccaggtcgcc gggcgcgtac
     2761 gcgccggtgg acaggtactt ccagccgccg tcgggcgaca gcacgaccac cttgccgccg
     2821 ggcgggacgc ggccggactc gcacacgcgg accgcgacgt gcagcgccgc gccggtggac
     2881 acgccggcga agatgccctc acggcgcacc aggtcccgcg tggcgcccag ggccttgagc
     2941 gagttcacct tgatgcggct gtccagcacg gtctggtcca gcaccggcgg gatgaagccc
     3001 tcgtcgatgt tgcgcagccc gtagacgagg tcgccgtact ccggctccgc cgcgaacacc
     3061 ttgatcgccg ggtcgtgctc cttcaggcgc cgcccgacgc cggtgagcgt gccgcccgtg
     3121 ccgaggccgc cgacgaacgc ggccacgtcc ggcaggtcgc gcaggatctc cggcgcggtg
     3181 gtctcgtaat gggcctgcgg gttggccggg ttgccgtact ggaacggcat gaaccagccg
     3241 gggtcggcct cggccatctc ccgcgcgacg cgcacgctgc cgttggagcc ctcgttggcc
     3301 ggcgagaaca ccagctccac gccgtacatg cccaggagct gtcggcgctc gtcgctgacg
     3361 ttctccggca tcacgcaggt cagcgcgtag cccttcgggg cgcacagggc ggccagggcg
     3421 atgccggtgt tgccgctggt gggctccagg atgcgcatgc cgggagtgag cttgccgtcg
     3481 cgctcggcct gctccagcag gtacttggcg acgcggtcct tcaccgaccc ggtggggttg
     3541 tggccctcca gcttgaggta gatctcgtag ccgtccggcg acaggttggg caggcggacg
     3601 agcggcgtgt cgccgacggc ctgggagacg tcgtccgcca gcacggggct agaaggctcc
     3661 gccggccacg gcgggcagga tgctcacctc ggcgccgtcg cgcagcggcg tctcgacacc
     3721 gcccaggtag cgcacgtcct cgtcgtccac gtagacgttg atgaagcggt gcagctgtcc
     3781 gtcctcaccg accaggcgcg ggccgatgcc ggggtagcgg gactccaggt cggcgaccag
     3841 ctcgcgcagg gtcgccccct gcccggtcac cttcttctcc cccagcgtgt gctcgcgcag
     3901 cacggtcggg atgcgcacct cgatggccat ggtcgaacgc tttcggtcgg gctcgtgtcc
     3961 ggctgacggg tcaggttaac ggttccccgc gggcgcctgt tccccatcca ccgtgagaac
     4021 tcgctccgtg atgcgcccgt cgcggatgtc gaacgcccgg atgacgggct cggggtcctg
     4081 cagcgacacg atgaggtaga cggcctccgg atacacggcc agttccacgt cggtgacgga
     4141 gggaaacgcc tcggtgtgtg tgtgggagtg gtagatgccg aactcccagt cgttgtcctc
     4201 gatctcggtg atggctttga ggtgcgcctt gccgtccatc ttgtagaagg tcatggagcg
     4261 ctcggcgttg gggatctcgt agtggcggtc gaagcgcccg ccggtcccgg cgaccatgcc
     4321 gcagacctcg taggggaagt cgctgcgcgc gtgcgccacc atctggtcgt agacctgccg
     4381 ggacaggtcc accgtgcctt cgatcgcagc cttggtcacc gtcgccgcat cctcgtctgt
     4441 cgtggtcagt gcaggtggag cgtgcccgaa cccggccggg cctcgccaat caccgcggcg
     4501 tcgtggccgc tgccgcgcag cctcgcgacc gcctcggcgg cccggtccgg cgcggcgccg
     4561 aacagcaggc cgccggaggt ctgcgcgtcc gacagcagca gccgggtggt ctcgtccgtg
     4621 gcgccggcgt cggtgaactc ggcgacccac tcgtggttgc ggcgcgtgcc gccgggcacg
     4681 acgccctgcg cggcgagctc gcgcgcgtgc ggcagcagcg gcacggcggc cgtgtccagg
     4741 tcaaggtcga cgcgcgaggc ggcggccatc ttgcgcaggt gccccagcat gccgaagccc
     4801 gtgacgtcgg tcgcgcccgt ggcg
//
LOCUS       DATMKC010000267         9602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_217203, whole genome shotgun sequence.
ACCESSION   DATMKC010000267 DATMKC010000000
VERSION     DATMKC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217203"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VNU01_07775"
     CDS             complement(<1..422)
                     /locus_tag="VNU01_07775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWH32556.1"
                     /translation="MEHDSVWLASARIPPREALAADLDADVVVVGAGTTGLTAALLAQ
                     RHGASVVVLESGRIAGGSTGRSTGKVTSQHTLWAASMIERHGEETARQYAQANQHAVE
                     LVGRLVGELGIDCRFERAFACAYTRDPRRRAAVEAEVEA"
     gene            557..1468
                     /locus_tag="VNU01_07780"
     CDS             557..1468
                     /locus_tag="VNU01_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH32557.1"
                     /translation="MKVFVAGATGVLGRRLLPRLVADGHDVTALARSDGGRRAVEAAG
                     ARPAQVSLFDLDALRTAIAGHEAVVNVATRIPTGPSAIRLAAWAENDRIRREGSANLV
                     DAALHAGAGRYVQESIAFVYADHGAGWIAEDGRLDLEFQLYSAVDAEAQAARFTAAGG
                     HGVALRFGLFLDETSAHYRDMTGMLRRGRIPAPGRADAYTATVMTDDAAAAMVAALRA
                     PAGLYNVVEDEPSTRAAHYAAAAAALGLPAPKPLPAAVGELPRVRVLARSQRVSNRAF
                     KEATGWAPRFSSARESWAELSRRAGAG"
     gene            1573..2442
                     /locus_tag="VNU01_07785"
     CDS             1573..2442
                     /locus_tag="VNU01_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32558.1"
                     /translation="MEIAEFLQHRRTVRTPVGEISYTDVGEGPAALFVHGVLVNGLLW
                     RNLVGQVADVRRCVAIDLPLHGGSPPTADMSFEGFADAVAALHEALDLGPVDLVGNDT
                     GGLVCQLFAAAHPGRLRTLTLTNCDVHDRVPPDAFKPAVELARQGQFARGLRAVLEQE
                     DPQALRDAFAIAYEHPERLPVELLRAYLEPVVGTPERGELFDRRLAAVDPDTLVRIEP
                     QLRGLDVPALLAWGTADEFFPLDDARTLLGILPRARLVTFEGAKLFFADERAAELAPH
                     VREHWLAHEEVAA"
     gene            2439..2909
                     /locus_tag="VNU01_07790"
     CDS             2439..2909
                     /locus_tag="VNU01_07790"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH32559.1"
                     /translation="MKDLRATGERRTVSIEVEAPPEDVYAFVTSVEKLGALGPECERC
                     EWVEPGVHFRGHNRVGDFTWSTDCFVVADEPGRRFAYEVGEPRYVRWTYELAPAASGT
                     RLAHSFEILHLSPIYEHVPVDHLPMRWAALEDGMRAVLAGVKREVEAARDAQPA"
     gene            2954..3580
                     /locus_tag="VNU01_07795"
     CDS             2954..3580
                     /locus_tag="VNU01_07795"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH32560.1"
                     /translation="MTEPRRNRKVEQGEQTRVALLAAARELFAAQGFAATSTEEIVRR
                     AAVTRGALYHHFADKEAIFRAVFEQIEAEMCGVVLAAAAKGADPVDRLLRGTRAYLDL
                     CTEPTVRRIALQEGPAVLGWETWHEIDSAYFFGILAGGVREAMRAGQIAKQPLEPLTH
                     LLVGAIMQAAMVLARAEDVQSEKRRLERSFRRLVDGLRAPAGGAAQAR"
     gene            complement(3542..4000)
                     /locus_tag="VNU01_07800"
     CDS             complement(3542..4000)
                     /locus_tag="VNU01_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWH32561.1"
                     /translation="MDADAAARLAEAFAEAWARPSLAAFRPLLADDVRLSAPFMDDTA
                     GVEEGLGELARLLRLWPDVRGEVHRWGATADGVLIEWTMRGRLGRADVAVPAVDRIVI
                     RDGLIAERVAYFDTLPLLAAALRQPRLWPAVLRSGLGPSARLRRAARRRA"
     gene            4108..5799
                     /locus_tag="VNU01_07805"
     CDS             4108..5799
                     /locus_tag="VNU01_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWH32562.1"
                     /translation="MSSGQDERDYDVIVIGSGFGGSVAALRATEKGYRVAVLEAGRRF
                     TEDTLPKTSWDARNFLWAPKLGWLGIQRMYLLKNVLVLSGAGVGGGSLVYANTLYEPL
                     EPFYADRQWAHITDWRAELAPHYDQAKRMLGVVESPADTPSDLVLRKVAERMGVGDTW
                     HPTNVGVFFGDGPGVESPDPFFGGAGPSRAGCTQCGSCMTGCRFRAKNTLDRNYLYLA
                     EQGGAQIHPETTVTAVRPDGAGGWLVDTVRTGRRGRRRDPRTLRAQQVVFAAGALGTQ
                     RLLHAMRDQGVLPNLSPRLGELTRTNSEAILGAMARNRKTDYSRGVAITSSMHPDDTT
                     HIEVVRYGPGSNAMGLLATVMTDGGGRVPRWLKWLGQVLRHPVIALRSLSVYHWSERT
                     IIALVMQSLDNSLTVFQTRRGRLTSRQGHGEPNPTWIPAGNEAVRLMAEEIGGEPGGS
                     VSEIANIPVTAHILGGCAIGDSPETGVVDPYQRVYGYEGLHVVDGAAVTANLGVNPSL
                     TITAQAERALAFWPNKGEADPRPGLGEPYRRVAPVPPRHPAVPADAPAALRIPIA"
     gene            6477..6965
                     /locus_tag="VNU01_07810"
     CDS             6477..6965
                     /locus_tag="VNU01_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32563.1"
                     /translation="MSLEPVATIGVPRPVAGCLAQLVAALGGPVTLIGGWAAEPVRLE
                     ADEQTGESVLVALPRAGALFAAKMANLDLEDRPPKKRAGDAEDAVRLLSTFGALALAG
                     DLGRATEAERLRLRYLVGATRASGLLAAVRNADVERDEERLGGALAAFVDDLNRDRRS
                     VV"
     gene            6962..7684
                     /locus_tag="VNU01_07815"
     CDS             6962..7684
                     /locus_tag="VNU01_07815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWH32564.1"
                     /translation="MKIDAPLGTGYALDTVKDFAVAAERDGYDGVWTIEAQHDPFLPL
                     VLAAEHTERLELGTGIAVAFARTPMSLAYVADDLQRMSGGRFILGLGSQIKPHIERRF
                     AMPWSKPAARMREFITAIRAIWRSWETGEPLRFTGEFYSHTLMTPFFSPGPNPHGNPP
                     VFLAAVGEVMTRVAGEVADGLLVHGFTTPRYLAEFTIPKLEEGLAASGRTRADTEVTH
                     AGFVATGASEQAMEAAKAGVLP"
     gene            7721..7996
                     /locus_tag="VNU01_07820"
     CDS             7721..7996
                     /locus_tag="VNU01_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32565.1"
                     /translation="MLELHGWGDVADELTRLSKENRPESWAAMSGLISDEILETFAIV
                     AEPAGVAAEVRARYGGLVDRYSFYAPYDAEPGLWGPIVAELREASSP"
     gene            complement(7993..8145)
                     /locus_tag="VNU01_07825"
     CDS             complement(7993..8145)
                     /locus_tag="VNU01_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32566.1"
                     /translation="MAGITDTIRVEHYGNPIDELSLDDVQPELEDCGAGLSDDSLLHW
                     PVLAER"
     gene            8259..8816
                     /locus_tag="VNU01_07830"
     CDS             8259..8816
                     /locus_tag="VNU01_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32567.1"
                     /translation="MCRGVRRVLLASTAAVLLVGCDPTVRATPPIQELSAVEAAVARS
                     QVTGSARTATTMPPGVFPTLRVTGVVDFRHDRSQTLLEIARPDGRASPYVDGISVLRA
                     VREQHGELQPAGDERDEWERAGGTTPGPDNLVGILIEAMSLDRDGRVGTVDFTLPAMR
                     RHGAFSMTFSEYGQAPPVRLPAVGQ"
     gene            complement(8832..>9602)
                     /locus_tag="VNU01_07835"
     CDS             complement(8832..>9602)
                     /locus_tag="VNU01_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH32568.1"
                     /translation="NHLNWSRIAEELGGEVTLLAPDLRGRGGSAALPGPYGMGAHADD
                     MIAVLDHFGIGRALFAGHSMGAFVMAIASARRPERTTGVLLIDGGFKLGEVPEGVDID
                     VILQAVIGPALERLKTTFPSREAYRDFWRAHPALAGDWSEHLEAYVDYDLTGEEPELR
                     SRTSLEAVREDGRTSLLESEIDTAPGRLPEPALFLRAPLGMLGAEPPLYPDEAVEAIR
                     AQSPQLQVELVPDVNHYTITMGKPGAAIVADRIRDLVG"
BASE COUNT         1295 a         3394 c         3603 g         1310 t
ORIGIN      
        1 gcctcgacct ccgcctcgac ggcggcgcgc cggcgcgggt cgcgcgtgta cgcgcaggcg
       61 aacgcgcgct cgaaccggca gtcgatgccg agctcgccca ccagtcgccc gaccagctcc
      121 acggcgtgct ggttggcctg cgcgtactgc cgtgccgtct cctcgccgtg gcgctcgatc
      181 atggacgcgg cccacagcgt gtgctggctg gtgaccttgc cggtggaccg gccggtgctg
      241 cccccggcga tgcgcccgct ttccagcacc accacggacg cgccgtggcg ctgtgccagc
      301 agcgccgccg tgaggccggt ggtgccggcg ccgaccacca ccacgtcggc gtcgaggtcg
      361 gccgccagcg cctcgcgcgg cgggatgcgg gcgctggcca gccatacgct gtcgtgctcc
      421 atgaccggca cttgcccgcc gcgccgacgc tctgaaaccg gccgacgttg ctttttctcc
      481 cgtcgcggcc caggaatgtc acggaccgca cggcggtttc gtcaacctgg ggccgaacca
      541 gggagaggag accaccatga aggtgttcgt cgcgggcgcc acaggggtgc tcggccgcag
      601 gctgctgccg cggctcgtgg cggatggtca cgacgtgacc gcgctggcgc gcagcgacgg
      661 cggccgtcgc gcggtggagg cggcgggggc gcggccggcc caggtctccc tgttcgacct
      721 cgacgcgctg cgcacggcga tcgccgggca cgaggcggtg gtcaacgtcg cgacgcgcat
      781 ccccacgggc ccgtccgcca tccgcctggc cgcgtgggcg gagaacgacc gcatccgccg
      841 tgagggctcg gcgaacctgg tggacgcggc gctgcacgcg ggcgctggcc gctacgtgca
      901 ggagtccatc gcgttcgtct acgccgacca cggcgccggc tggatcgccg aggacggccg
      961 cctggacttg gagttccagc tctactccgc ggtggacgca gaggcgcagg ccgcgcgctt
     1021 caccgccgcc ggcggccacg gcgtggcgct gcgcttcggg ctgttcctgg acgagaccag
     1081 cgcacactac cgggacatga ccggcatgct gcggcgcggg cgcatcccgg cgccgggccg
     1141 cgccgacgcc tacacggcga cggtgatgac cgacgacgcc gcggcggcca tggtggcggc
     1201 cctgcgcgcg ccggccggcc tgtacaacgt cgtggaggac gagccgtcca cgcgcgcggc
     1261 gcactatgcc gccgcggccg ccgccctggg gcttcccgcg ccgaagccgc tgcccgccgc
     1321 cgtgggcgag ctgccgcgcg tgcgcgtgct ggcccgctcc cagcgcgtgt cgaaccgggc
     1381 gttcaaggag gcgacgggct gggcgccgcg cttctccagc gcgcgggaga gctgggccga
     1441 actctcgcgc cgcgccgggg ccggctgagg cgcggcatag cggcctcgcg cgtggttgac
     1501 atacatacag cttgtatgta tgtttccgca cggaaggttt catcacaaga cccacacgcc
     1561 gagggggcgg acatggagat tgccgagttc ctgcagcatc gccggaccgt caggaccccg
     1621 gtcggcgaga tctcctacac cgacgtgggg gaggggcccg cggcgctgtt cgtgcacggc
     1681 gtcctggtca acggcctgct gtggcgcaac ctcgtcggcc aggtggccga cgtccgccgc
     1741 tgcgtcgcca tcgacctgcc cctgcacggc ggcagcccgc ccacggccga catgtccttc
     1801 gagggcttcg ccgatgccgt ggcggccctg cacgaggcgc tcgacctggg gccggtggac
     1861 ctggtgggca acgataccgg cgggctggtg tgccagttgt tcgccgcggc gcaccccggt
     1921 cggctgcgca cgctgacgct cacgaactgc gacgtgcacg accgcgtgcc gccagacgcg
     1981 ttcaagccgg cggtcgagct ggccaggcag ggccagttcg cgcgcgggct gcgcgccgtg
     2041 ctggagcagg aggatcccca ggcgctgcgc gacgccttcg ccatcgccta cgagcaccct
     2101 gagcggcttc cggtggaact gctgcgcgcg tacctggagc ctgtcgtcgg cacgcccgag
     2161 cgcggcgagc tgttcgaccg gcgcctggcc gccgtggacc cggacacgct ggtgcgcatc
     2221 gagccgcagc tgcgcgggct cgacgtgccg gcgcttttgg cctggggcac cgccgacgag
     2281 ttcttcccgc tggacgacgc gcggacgctg ctcggcatcc tgccgcgggc gaggctggtc
     2341 accttcgagg gagccaagct gttcttcgcc gacgagcgcg cggcggagct ggcgccgcac
     2401 gtgcgggagc actggctggc gcacgaagag gtggcggcat gaaagatctg cgcgcgaccg
     2461 gcgagcggcg caccgtcagc atcgaggtgg aagcccctcc ggaggacgtg tacgcgttcg
     2521 tgacgtcggt ggagaagctc ggtgcgctgg gcccggagtg cgagcgctgc gagtgggtcg
     2581 agccgggcgt gcacttccgc ggccacaacc gcgtcggtga cttcacctgg agcacggact
     2641 gcttcgtggt ggcggacgag ccgggcaggc gcttcgccta cgaggtgggc gagccgcgct
     2701 acgtgcgctg gacctacgag ctggcgccgg cggcttccgg cacccggctc gcgcacagct
     2761 tcgagatcct gcacctgagc cccatctacg agcacgtgcc cgtcgaccac ctgccgatgc
     2821 ggtgggcggc cctggaggac ggcatgcgcg cggtgctggc cggcgtcaaa cgcgaggtcg
     2881 aggccgcccg cgacgcgcag ccggcgtagc ggcacgacgg gccggggcag gcggcgcctc
     2941 taccatgtgg cgcgtgaccg agccgcggcg caaccgcaag gtggagcagg gcgagcagac
     3001 gcgcgtcgcg ctgctggccg ccgcgcgcga gctgttcgcc gcgcaggggt tcgcggccac
     3061 cagcacggag gagatcgtgc gccgcgccgc cgtcacccgc ggcgccctgt accaccactt
     3121 cgccgacaag gaggcgatct tccgggcggt gttcgagcag atcgaggcgg agatgtgcgg
     3181 cgtggtgctc gccgcggccg ccaagggcgc cgacccggtg gaccgcctcc tgcgcggcac
     3241 gcgcgcctac ctggacctgt gcacggagcc gacggtccgg cgcatcgcgc tccaggaggg
     3301 cccggcggtg ctggggtggg agacctggca cgagatcgac agcgcctact tcttcggcat
     3361 cctggccggc ggcgtccgcg aggcgatgcg ggccggccag atcgccaagc agccgctgga
     3421 gccgctgacg cacctgctcg tcggcgcgat catgcaggcc gcgatggtgc tggcgcgggc
     3481 ggaggacgtc cagtcggaga agcgccggct ggagcgctcg ttccgccgcc tggtcgacgg
     3541 gctacgcgcg ccggcgggcg gcgcggcgca ggcgcgctga gggtcccaaa ccggagcgca
     3601 gcacggccgg ccacagccgc ggctgccgca gcgcggcggc cagcagcggc agcgtgtcga
     3661 agtaggcgac gcgctcggcg atgaggccgt cgcggatcac gatgcggtcc accgccggca
     3721 ccgcgacgtc ggcgcggccc aggcggccgc gcatcgtcca ctcgatcagc acgccgtcgg
     3781 ccgtggcgcc ccagcggtgc acctcgccgc gcacgtcggg ccacagccgg agcaggcggg
     3841 ccagctcgcc caggccctct tcgacgccgg cggtgtcgtc catgaacggg gcggacaggc
     3901 gcacgtcgtc ggccagcagc gggcggaacg ccgccaggct gggccgggcc cacgcctcgg
     3961 cgaaggcctc cgccagccgc gcggccgcgt ccgcgtccat gcgcacaccc ccttctgcca
     4021 cgaatccgtg gcgcaatcac gcgcgcggga cactagcatc agggagccgg tgacggccgg
     4081 tcacgggcgg ggacaggagc cggggcgatg agcagcgggc aggacgagcg ggactacgac
     4141 gtcatcgtga tcggcagcgg cttcggcggc agcgtcgcgg cgctgcgcgc cacggagaag
     4201 ggctaccgcg tcgccgtgct ggaggcgggg cggcgcttca ccgaggacac gctgcccaag
     4261 acgagctggg acgcgcgcaa cttcctgtgg gcgcccaagc tcgggtggct cggcatccag
     4321 cgcatgtacc tgctgaagaa cgtgctggtg ctgtccggcg ccggcgtcgg cggcggctcc
     4381 ctggtgtacg ccaacacgct ctacgagccg ctcgagccgt tctacgccga ccggcagtgg
     4441 gcccacatca ccgactggcg cgccgagctc gcgccgcact acgaccaggc caagcgcatg
     4501 ctcggcgtgg tggagagccc ggcggacaca ccgtcggacc tggtgctgcg caaggtcgcc
     4561 gagcgcatgg gcgtcgggga cacctggcac cccaccaacg tcggcgtgtt cttcggcgac
     4621 ggtcccggcg tggagtcgcc ggacccgttc ttcggcggcg ccggaccgtc gcgggcgggc
     4681 tgcacgcagt gcggctcctg catgaccggc tgccgcttcc gcgccaagaa caccctggac
     4741 cgcaactacc tgtacctggc ggagcagggc ggcgcgcaga tccacccgga gaccacggtg
     4801 acggcggtgc gccccgacgg cgccggcggc tggctggtcg acacggtgcg caccggccgc
     4861 cgcgggcgcc gccgggaccc gcgcacgctg cgcgcccagc aggtcgtctt cgccgccggc
     4921 gcgctgggca cgcagcggct gctgcacgcc atgcgcgacc agggggtgct gccgaacctg
     4981 tcgccgcgcc tgggggagct cacccggacc aactccgagg cgatcctggg cgcgatggcg
     5041 cgcaaccgca agacggacta cagccgcggc gtggcgatca cctcgtcgat gcacccggac
     5101 gacaccacgc acatcgaggt ggtgcgctac gggccgggct ccaacgcgat ggggcttttg
     5161 gccacggtga tgacggacgg cggcgggcgc gtcccgcgct ggctgaagtg gctggggcag
     5221 gtgttgcgcc atcccgtgat cgcgctgcgc tccctgtcgg tctaccactg gtcggagcgc
     5281 acgatcatcg cgctggtgat gcagtcgctg gacaactccc tgaccgtgtt ccagaccaga
     5341 cgcggccggc tcaccagccg ccagggccac ggggagccga acccgacgtg gatccccgcc
     5401 ggcaacgagg cggtgcgcct catggcggag gagatcggcg gcgagccggg cggcagcgtg
     5461 agcgagatcg cgaacatccc ggtgacggcg cacatcctgg gcggctgcgc gatcggggac
     5521 tcgccggaga ccggcgtggt ggacccgtac cagcgcgtgt acggctacga gggcctgcat
     5581 gtggtggacg gcgccgccgt gaccgcgaac ctgggcgtga acccgtcgct caccatcacg
     5641 gcgcaggcgg agcgcgcgct ggcgttctgg ccgaacaagg gcgaggccga tccgcgaccg
     5701 ggcttggggg agccgtaccg gcgggtcgcg ccggtgccgc cgcgccaccc ggcggtgccc
     5761 gccgacgccc ccgcggccct gcgcatccct atcgcttgat cggccccaca tgaaaccaca
     5821 tgttctcggt atggaccggt tattacactt gactctccat gtaataagtt gttggctata
     5881 cctatgaagc ccacagtcgg cctgccagtg cccgcgacga ggaacccctt cgccgggaag
     5941 ggtctcaggg tcagtctcgc cctggtgctt gaccctcgcc cttggtctgt gacggagctt
     6001 gctcgcgcgg cagatgtgag ccagcccatg gcctcactgg tcatgagaga actgaggcac
     6061 cgcggactcg tacggggcga gtcagctcgc gggcgtgccg ctgcgctcta cccgacggcc
     6121 gcgctgctct gggagttggc attgcattgg ccgagaccgc aggccggtgt gcaaggccgg
     6181 ttgcccgttg acggcccgat cggcggtggc ccagcgcatc gtgcggcggg gctggtgacc
     6241 gatgcggtgc cgattcggta tgtacgcgac cgtgctgaag ctgtcgcgct tctcggcagg
     6301 tggggcggag cctgggtaag ccgcgaggtc gcggactacg acatctgtgt cctcgacgtc
     6361 gatctgccta cgggacttgt gccagacggc atcgtggctc tcgaactggg tgggacgcct
     6421 cgcgggcgcg aggtcttgga gatgcatgtc gctgagctgg ttccggagtt agccgcatga
     6481 gcctggagcc agtcgccacc atcggggtgc caagacccgt cgctggatgc ctcgctcaac
     6541 tcgtggcggc tttgggcggc ccggtcacac tcatcggcgg ttgggcggca gagcccgtgc
     6601 gccttgaggc ggacgaacag acgggtgagt cggtcctcgt tgcccttcct cgtgcaggcg
     6661 cgctcttcgc ggcaaagatg gcgaacctcg atctggagga ccggccaccc aagaagaggg
     6721 ccggtgacgc agaggacgcc gtgcggctgc tgagcacctt tggggcgctg gctctggcag
     6781 gcgatctcgg ccgcgccacc gaggcagagc gactccgcct ccggtatctc gtgggcgcga
     6841 cccgggcgag cggcctgttg gcggctgtgc gcaatgcgga cgtggaacgg gatgaggagc
     6901 ggctcggggg tgcgctcgcg gcgttcgtcg atgatctgaa ccgagaccgg cggtcagtcg
     6961 tgtgaagatc gacgcgccgc tgggcaccgg ctacgcgctg gacaccgtga aggacttcgc
     7021 cgtggccgcg gagcgcgacg gctacgacgg cgtgtggacc atcgaggcgc agcacgaccc
     7081 gttcctgcca ctggtgctgg ccgccgagca caccgagcgc ctggagctgg gcaccggcat
     7141 cgcggtggcg ttcgcgcgca ccccgatgag cttggcctac gtcgccgacg acctgcagcg
     7201 catgtccggc ggccgcttca tcctgggact gggcagccag atcaagccgc acatcgaacg
     7261 gcgcttcgcg atgccgtgga gcaagcccgc cgcgcgcatg cgcgagttca tcaccgcgat
     7321 ccgcgcgatc tggcgcagtt gggagaccgg ggagccgctg cgcttcaccg gcgagttcta
     7381 cagccacacg ctcatgacgc cgttcttctc gccgggcccc aacccgcacg gcaacccgcc
     7441 ggtcttcctg gccgcggtgg gcgaggtgat gacgcgggtg gcgggcgagg tcgccgacgg
     7501 cctgctggtg cacggcttca ccacgccgcg ctacctggcc gagttcacga tccccaagct
     7561 ggaggagggg ctggccgcgt cggggcgcac gcgcgcggac acggaggtca cccacgcggg
     7621 cttcgtcgcc acgggcgcga gcgagcaggc gatggaggcg gccaaggccg gcgtgctgcc
     7681 gtagaacgcc aggtgctggc gcacgccggc gtacaagccg gtgctggagt tgcacggctg
     7741 gggggacgtc gccgacgagc tgacccggct gtccaaggag aaccggccgg agtcctgggc
     7801 ggcgatgagc gggctgatca gcgacgagat cctggagacg ttcgccatcg tggccgagcc
     7861 cgccggcgtg gcggccgagg tccgggcgcg ctacggcggc ctggtggacc gctacagctt
     7921 ctacgcgccg tacgacgccg agcccggcct gtgggggccg atcgtggcgg aactgcgcga
     7981 ggcctcgagc ccctagcgct ccgccagcac gggccagtgc aggagcgagt cgtcgctcag
     8041 gccggcgccg cagtcctcga gctcgggctg cacgtcgtcc aggctcagct cgtcgatcgg
     8101 gttgccgtag tgctcgacgc ggatcgtgtc ggtgattcct gccatggggg tggctccttg
     8161 ccgtgggtcc accgacgatg aacccctacc gcgcggtagg caatggcccg gatcggccat
     8221 gcgtaccatg gccgcgagct cggacgggga ggcggccggt gtgcagggga gtgcggcggg
     8281 tgctgctggc ctcgactgcg gcggtgctgc tggtgggctg tgaccccacc gtgcgggcca
     8341 caccgccgat ccaggagctg tccgctgtgg aggcggccgt cgcgcggagc caggtgacgg
     8401 gcagcgcgcg caccgcgacg acgatgccgc cgggggtctt cccgacgctc cgcgtgaccg
     8461 gggtggtcga cttccggcac gaccgcagcc agacgctgct ggagatcgcg cggcccgacg
     8521 gccgggcctc gccgtacgtc gacgggatca gcgtgctcag ggcggtgcgc gagcagcacg
     8581 gcgagctgca gccggccggg gacgagcgag acgagtggga gcgcgccggc gggacgacgc
     8641 ccgggccgga caacctcgtc ggcatcctga tcgaggcgat gtcactcgac cgcgatggcc
     8701 gtgtgggaac cgtcgacttc acgctcccgg ccatgcgccg gcacggcgcc ttctcgatga
     8761 ccttcagcga gtacggccag gccccgcccg tccggcttcc cgccgtcggg cagtagggct
     8821 gtcggccggg ctcagccgac caggtcgcgg atgcggtcgg cgacgatggc ggcgcccggc
     8881 ttgcccatcg tgatggtgta gtggttcacg tcgggcacca gctccacctg gagctgcggc
     8941 gactgtgccc tgatggcctc caccgcctcg tccgggtaca gcggcggctc cgcgccgagc
     9001 atgcccagcg gggcgcgcag gaacagcgcc ggctccggca gccgcccggg ggccgtgtcg
     9061 atctcggact ccagcagcga cgtgcgcccg tcctcccgca ccgcctccag cgacgtgcgc
     9121 gagcgcaact ccggctcctc gcccgtgagg tcgtagtcca cgtaggcctc caggtgctcg
     9181 ctccagtcgc ccgccagggc ggggtgcgcg cgccagaagt cgcggtaggc ctcgcgggac
     9241 gggaacgtgg tcttgagccg ctccagggcc ggcccgatga ccgcctggag gatcacgtcg
     9301 atgtcgacgc cctccgggac ctcgcccagc ttgaagccgc cgtcgatgag cagcaccccg
     9361 gtcgtgcgct ccggccgccg cgccgacgcg atcgccatca cgaacgcgcc catggagtgg
     9421 cccgcgaaca gcgcccgccc gatcccgaag tggtcaagga ccgcgatcat gtcgtcggcg
     9481 tgcgccccca tgccgtacgg cccgggcagc gcggcgctgc cgccccgccc gcgcaggtcg
     9541 ggggccagca gcgtcacctc gccgcccagc tcctcggcga tgcggctcca gttgaggtgg
     9601 tt
//
LOCUS       DATMKC010000268        20116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_345724, whole genome shotgun sequence.
ACCESSION   DATMKC010000268 DATMKC010000000
VERSION     DATMKC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 20116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20116
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345724"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VNU01_07840"
     CDS             complement(<1..1106)
                     /locus_tag="VNU01_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family efflux MFS transporter"
                     /protein_id="HWH32569.1"
                     /translation="MTTLLSTSRPPRGTLGRRELTWFLALSMALAALGIDVMLPAFGA
                     IREGFGLPETSTAVTGVLTTYFLGLAAGQLLYGPIADRFGRKPTLGVGYAVYGLGALA
                     SALAPTLPALLAARFLWGIGAAGPRVVTQAVIRDTFEGEEMSRAMSMIMAVFILVPVI
                     APTMGAAVITVVSWRWLFAICVAAVLAVSVWSRRLQETLREEHRIPQLRFGRIAQAAR
                     LVVSNRQTVAYSLAMTFLWGAFTGYLGSSEIIVSEVYGLPTAFPVVFGGLALFMGAGM
                     LVNARVVRRLGVRRLAHGVLVAYIGLASVLAAIVFTAGGRPPFPLLLAALAAMLLCQA
                     LLVPNLNTIAMTPMAAIAGTAASVVGSVQIALGAT"
     gene            1279..2814
                     /gene="lysS"
                     /locus_tag="VNU01_07845"
     CDS             1279..2814
                     /gene="lysS"
                     /locus_tag="VNU01_07845"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWH32570.1"
                     /translation="MSDDHQQPPADGEGAPGESASRLDELIAERRDKLARWRERGVEP
                     YPVGVRVEHSLAAVRERWDAALSPGEETEERVSIGGRVVLRRGHGKLVFLVLREWDTD
                     LQVMCQLDVLGEPAMADVDELDVGDWVAVHGRVIASRRGELSVQAEQVTLIAKSLRPL
                     PDKWHGVRDIDTRFRQRYADLIVNAESRRVFEVRSRVLSALRAELAERGYVEVETPML
                     HPIPGGAAARPFVTHHNALDLDLYLRIAPELYLKRLVAGGMPRVFEISRVFRNEGLSP
                     RHNPEFTMLESYEAYADYTDVMALTQALIQRAAMEALGQTAVVYDGREVDLGGDWPRY
                     TLLDLVREVTGRADLSYDMDLAELRKVCDDYGVAAEEPWGPGKLVLELYEKLVEHTLW
                     EPTFVVDYPVEVSPLARRHRDDPFLTERFELIVVGRELANAFSELTDPEDQRARLEAQ
                     ALAKAGGDEEAMVVDEDYLRAMEYGMPPMGGLGVGVDRLVMLLADVQTIRDVILFPTL
                     RPE"
     gene            3236..5743
                     /locus_tag="VNU01_07850"
     CDS             3236..5743
                     /locus_tag="VNU01_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HWH32571.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEGVREQVEEIIGQGQTAPAGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQQVIQLLSGYAGGEAGQGAKAGGGT
                     AMAEDPKGSPVLDQFGRNLTQLAREGKLDPVIGRTREIERVMQVLSRRTKNNPVLIGE
                     PGVGKTAIVEGLAQMIVNAQVPETLKDKQLYTLDLGALVAGSRYRGDFEERLKKVLKE
                     ITTRGDIVLFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTIEEYRKHLE
                     KDAALERRFQPIKVEEPSVAHTIEILKGLRDRYEAHHRVTITDDALVAAGNLADRYIS
                     DRFLPDKAIDLIDEAGSRLRIRRLTAPPDLKELDDEAAQVRKAKEAAIDEQDFEKAAA
                     LRDQEKKILERKSNREREWKSDGMDTVLTLDEEDIAEVLSNWTGIPVFKLTEEETAKL
                     LRMEDELHKRVIGQEDAIKAVSKSIRRTRAGLKDPKRPSGSFIFLGPSGVGKTELAKT
                     LAEFLFGDEDSLIHLDMSEYMEKHTVSRLIGSPPGYVGYEEGGQLTEAVRRKPFSVVL
                     FDEVEKAHPDVFNALLQILEDGRLTDAQGKQVDFKNTVLIMTSNLGTRNLGKQGLGFT
                     AHEDERDTYERMKEQVNDELKKHFRPEFLNRIDETIVFHQLTREQVKSIVDLMIKRVR
                     EALKSKDLDIELTEDAKAWLADKGYDAQLGARPLRRTIQREIEDSLSEKILFGEFRAG
                     QLIVADVEDDAIVFRAVDSPPDAPPVELAGSGGNEQG"
     gene            6036..7313
                     /locus_tag="VNU01_07855"
     CDS             6036..7313
                     /locus_tag="VNU01_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWH32572.1"
                     /translation="MRKLMGHVVPLDGDRYRHWDTLRHCMPPEGLTPEEWWMATKLGR
                     EPLLRSLPLRDPEGKPLSVALPDPALEMLMRVDQQASGRIQAPDAVTNPATRDRYLVN
                     SLMEEAIRSSQLEGAATTRKAAKEMLRSGRSPRDRSERMILNNYLAMQFVREHVTEPL
                     TPELVLQLHRIVTDGTLEDPADAGRLQRPGEERIKIWWDGELVYEPPPAEQLEERLRL
                     LCDFANGGAIDGFLHPVARAVLVHFWLAYDHPFVDGNGRTARALFYWAMLSQGYWLAE
                     FLPISNIIRQAPARYAKSFLYVETDEGDTTYFLLSQLRILCRAIDELHAYLGRKTRQV
                     QEVEVLLRRSPGFNHRQIALLSHALRDAGAQYTFATHQRSHGVVYQSARTDLLDLAER
                     GLLQQTKRGRAYVFFPAADLERRLKALSGAESS"
     gene            complement(7321..7773)
                     /locus_tag="VNU01_07860"
     CDS             complement(7321..7773)
                     /locus_tag="VNU01_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32573.1"
                     /translation="MSTDETTRRGASAAAGSGTKRQLRVWLIILAVPAVVSAVWGIFG
                     PASWYADFGGPAAPSAFGVYNEHFVQDIGGGYLAFGAALVYAAVVLHRHAVRAALIAT
                     LGFSVPHLVIHLIDQGHLDTGGYLFVNATLLFGVAVALWCWRLSSRMD"
     gene            7980..8639
                     /gene="nucS"
                     /locus_tag="VNU01_07865"
     CDS             7980..8639
                     /gene="nucS"
                     /locus_tag="VNU01_07865"
                     /inference="COORDINATES: protein motif:HMM:NF002876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HWH32574.1"
                     /translation="MRLVIARCSVDYAGRLTSHLPSAVRLLMVKADGCVAVHADGGAY
                     KPLNWMNAPNTVTEEEGRWVVANPKGETLTITLEEVLHDSLHDLGEDPGLSKDGVEAH
                     LQELLAEHPDELQDGLTLVRREFPTDIGPVDLLCRDGDGQAVAVEVKRRGEIDGVEQL
                     TRYLERLNRDPMLAPVRGIFAAQVIKPQARVLAEERGIYCAEVDYDALRGIESDALKL
                     F"
     gene            complement(8626..9360)
                     /locus_tag="VNU01_07870"
     CDS             complement(8626..9360)
                     /locus_tag="VNU01_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWH32575.1"
                     /translation="MRRLLPDPADDVDVYDAYRVDDGAAHLRVNMVLSADGRAQDRDG
                     RTAALGGAGDQMVFRTLRALADGVLVGAGTVRAEGYGPHRLREELHERRIADGRPLPA
                     QIVVVSRSLDLDYGAPLFTEAKAPTVVLTCEAAPEDGRNAAARAGVLLIAGEDEVDLA
                     LGIAALRERFGMAHLLCEGGPTLNAPLFDAGLADELCLTVAPVMVGAQAASLVGELSA
                     ARPLTLTHALEDAGELYLRYAVARTA"
     gene            9414..10319
                     /locus_tag="VNU01_07875"
     CDS             9414..10319
                     /locus_tag="VNU01_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWH32576.1"
                     /translation="MPHSPPRARALRFQRLLLDWYAAQARALPWRRTRDPYRVLVSEI
                     MLQQTQVSRVEPLYRAFLRRFPSARALAAAPAAEVLGAWRGLGYNRRALNLHRAAQAV
                     VQRHGGRVPGDLEALRALPGVGDYTARAVLAFAFGRDAAPVDTNVARVLARAVAGAPL
                     GKAQAQRLADAVLPRGRAAEWGNALMDLGARVCTARLPRCEDCPVASACAWRRAGGAD
                     PAAAGALRPRPQAPFAGSDRFHRGRLVDALRDGPLLPGALGRAAQLDDAARLEGIVAA
                     LVREGMAQWAGGVLRLPRAEAAPRA"
     gene            10392..11270
                     /locus_tag="VNU01_07880"
     CDS             10392..11270
                     /locus_tag="VNU01_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWH32577.1"
                     /translation="MTMDAATLKRLGKQVGAEVKADNVGLLASAVAFRIFLSIFPSLI
                     AVVGVFSLVTSPSDITGLINEARGVLPSEAQGFLERTLTRMVAEGSGGGFAIAGVLGG
                     LWAASSAAAVLIKALNAAYEVAESRGFVKQRLVALLLTGVLLLAIFGLLALLVVGRFL
                     QAWLVPDSLQGPVVDTVFLLGRVAGAVLLMMVLFAVVYRVGPNRGRAAPFEWVTPGAV
                     LAVLAWLALSALFALYTRAFGSYGATYGSLAGAVVMMLWLQLTMTTLLVGAELNQVWR
                     RHTRARDGAGSRVEPD"
     gene            11307..12668
                     /gene="radA"
                     /locus_tag="VNU01_07885"
     CDS             11307..12668
                     /gene="radA"
                     /locus_tag="VNU01_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWH32578.1"
                     /translation="MAKTRSQARCGECGHVEAKWMGRCPACGAWGSMAEEARPAASSA
                     AAGRAAAPVRAAVPIAQVPLDGDRRAATGIGELDRVLGGGLVPGSVTLVGGEPGAGKS
                     TLLLQAADALARRGAAVLYVSAEESPSQVRMRAERLGALSERLLLGSETDLAAVLGLL
                     DLHKPDVLVLDSIQTVTDADTAGTAGGVSQVRACAGAIVRACKESGIAGVLVGHVTKE
                     GQLAGPRTLEHVVDTVCELSGDRHHALRLLQAVKHRYGAVGEVGCFTMTGRGMVGVDE
                     AGRLFVGEAPDGTTGVAVTLALEGRRPLACEVQALTVPTTAANPRRVASGLDPQRLSL
                     LLAVLEARAEVKLSTDEVFASSVGGVRLSEPAVDLALCLAVASAKKSKPVPRRWVVLG
                     EVGLAGELRLVAQTERRLAEAARLGFRGAILPEAYDGDAFGLRLHKAPDLGGALAVGL
                     AAH"
     gene            12695..13762
                     /gene="disA"
                     /locus_tag="VNU01_07890"
     CDS             12695..13762
                     /gene="disA"
                     /locus_tag="VNU01_07890"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HWH32579.1"
                     /translation="MAPRDDRLLAVLQKVAPGTALREGIDRIIRAGKGAIIVLGDGEE
                     IDRIVSGGFRLEAKFTAQRLSEVAKMDGAIILDNTVERILYANVHLVPDPSIPTAETG
                     TRHRTAERVAKQTARPVISVSESMRIVSLYVDAQKHTLEEISSILFRANQALATLERY
                     RMRFDEVTAGLSALEIENVATVRDVVSVLQRAEMVRRIAEEIDAYIAELGTDGRLLQL
                     QLDELLAGIEGERMLIVRDYMPDRRRKVESVLGDLHAIPQQDLLELARVADAVGLGTG
                     EGKLDEPISPRGYRLLSRIPRLPDRMVDRLVSRFSSLQRLMEATLDDLDEVEGIGEAR
                     ARSIKEGLARLAESSILERYA"
     gene            13934..14416
                     /locus_tag="VNU01_07895"
     CDS             13934..14416
                     /locus_tag="VNU01_07895"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWH32580.1"
                     /translation="MTYRVGDTVVYPHHGAAVIEKREKRTLAGQERDYLVLRLTYGDL
                     TLMVPADATEEVGLRSVVTKKEVDEVLKVLKKRDGNMPTNWSRRFKANYEKLRSGDIY
                     QVAEVVRNLAHREAEKGLSAGEKRMLTKSKQILVSELSAALKKSDEDTEVLIDEVLGT
                     "
     gene            14482..15522
                     /locus_tag="VNU01_07900"
     CDS             14482..15522
                     /locus_tag="VNU01_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HWH32581.1"
                     /translation="MVVAAIGGAYALGEWAAFELPPEGVLGSDWFPVVVLVLAASVAY
                     LVGGAVGRAVHGGVDLAERRLRRVPTGELLAGAIGAVAGFLLTLAVVWPVLLFEGRVV
                     TVPLAVLVAVVLTSAGARIGRARGGDFLRFMGVPGRLRVSSPAHGGGVKLVDSSALVD
                     GRLLDVCRAGFVEGTLVVPRFVLAEVQGLADAGDEQRRTRGKRALDVLAALQRSAGVA
                     LEVPDDDYPEVADVDAKLLAMARDIDAALVTVDGNLGRVAEVQGIRVLNLHTLAETLR
                     PPVLVGDTVRLRLTRPGREPGQGVGHLDDGTMVVVEGGAAHVEDDVQAEITSVLSTAN
                     GRMLFARLGEDR"
     gene            15519..16202
                     /gene="ispD"
                     /locus_tag="VNU01_07905"
     CDS             15519..16202
                     /gene="ispD"
                     /locus_tag="VNU01_07905"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWH32582.1"
                     /translation="MTTTAAVLVAGGAGERLGLPQPKAFVPLAGEPMLARAARALSAA
                     GVFARIVVVVPEGWQERARELCADVDAELTVCAGGRTRQESVARGVAHAAEDVVAVHD
                     AARALVPPELVRDVVAALTDQWDAVAPAVPIVDTVKRVDPDDGRVLETVGRATLRAVQ
                     TPQVFRRDLLTRLHGEAHGADATDDLLLVEQAGGRVRLLDGDVRNLKVTTAADLLFAE
                     ALLREDARA"
     gene            16199..16717
                     /gene="ispF"
                     /locus_tag="VNU01_07910"
     CDS             16199..16717
                     /gene="ispF"
                     /locus_tag="VNU01_07910"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWH32583.1"
                     /translation="MRIGQGVDAHRFAEEGAGRPLRLCGVEIPDAPGLEGHSDADVAL
                     HAVADAILGAAALGDLGALFGTNDERYAGADSAVFLHDAVHRAAQFGLRIGNVDCTLV
                     AQRPRLSAHLPAMRERLAELLGADPAAVSVKATTTDGLGFTGRSEGIACLAVVLLGPV
                     DPAQATAHPTAR"
     gene            16749..18131
                     /gene="cysS"
                     /locus_tag="VNU01_07915"
     CDS             16749..18131
                     /gene="cysS"
                     /locus_tag="VNU01_07915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWH32584.1"
                     /translation="MRLTNTLTNREEEFTPLRDDLVTMYVCGPTVQGPPHFGHARAAI
                     VPDVLRRYLEWSGHRVFHVRNITDVDDKIIARAQEEGRDFFEVGEHFARVWDQQITRL
                     NVLWPHVVPRATGHIPEMIALITRLIANGAAYPSGGDVYFRVRAFPEYGKLSNRNVDE
                     LRAGARIEPGEHKEDPADFALWKAAKPGEPSWDSPWGPGRPGWHIECSAMSSKYLGET
                     FDIHAGGVDLVFPHHENEVAQSEAATGRQFARYWVHNGLLTLGAEKMSKSVGNIISLD
                     EAIATYGGDVLRFFYLAAHYRSPVEFSEERLSEAQAAIERWRTFLRLVPEGDGAAVDT
                     PGGDEPGTYRDRFRAALDDDLSTPQAHAVLFDLATAGLAHAEAGRSQEASALRALFVE
                     LARDVMGYRLEDRAAGDLVGPLVEELLALRQEARARKDFATSDRVRDRLAGLGVVIED
                     TPGGSRWHLA"
     gene            18246..18920
                     /gene="rlmB"
                     /locus_tag="VNU01_07920"
     CDS             18246..18920
                     /gene="rlmB"
                     /locus_tag="VNU01_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWH32585.1"
                     /translation="MPRHGVREVLVERLDGNEEILELARGRKVPVRRVAREELDAAAQ
                     GVLHQGVVAVAPPFAYVDLHQAATGDLVVVLDGVTDPQNLGAIARSAEAAGARGLVLP
                     KRRSAHVTAAAEKAAAGALSWLPVALVPNIVRALEELAERGFWSVGLAGEAGSALWDS
                     GLLDGKVALVVGAEGGGLSRLVQERVDERLQIPMTGHAESLNASAAAAIALFEIARRR
                     HQRTDI"
     gene            19085..19735
                     /gene="sigH"
                     /locus_tag="VNU01_07925"
     CDS             19085..19735
                     /gene="sigH"
                     /locus_tag="VNU01_07925"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HWH32586.1"
                     /translation="MSLEIVIDLTEARTEDDDVLVARAWNGDESALGTLLDRYRGFVR
                     GKARSYFLAGADREDVVQEGMIGLYKAIRDYDPARQSTFRAFADLCVTRQIITAVKTA
                     TRQKHGPLNSYISFSLPAHGDDSDQELVDCLPAAAEDPCERVVAGHDLAELERWFGEI
                     LSDLEAEVLQRYVSGESYAEIAAALDRHVKSIDNALQRIKRKLDLYLQARSFEPAV"
     gene            19877..20116
                     /locus_tag="VNU01_07930"
                     /pseudo
     CDS             19877..20116
                     /locus_tag="VNU01_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         2854 a         7189 c         7370 g         2703 t
ORIGIN      
        1 gtcgcgccga gcgcgatctg caccgacccc accacggacg cggccgtgcc cgcgatcgcc
       61 gccatcggcg tcatcgcgat ggtgttgagg ttgggcacca gcagcgcctg gcacagcagc
      121 atcgcggcca gcgccgcgag cagcaggggg aagggcggac ggccgccggc ggtgaagacg
      181 atcgccgcga gcacggaggc cagaccgatg taggcgacca gcaccccgtg cgcgagcctg
      241 cgcacgccca agcggcgcac cacgcgcgcg ttgacgagca tgccggcgcc catgaacagc
      301 gccagcccgc cgaagacgac cgggaacgcg gtcggcaggc cgtacacctc gctcacgatg
      361 atctcggagc tgcccaggta gccggtgaac gccccccaca ggaacgtcat cgccaggctg
      421 taggcgaccg tctgccggtt ggacaccacc agcctggcgg cctgcgcgat gcgcccgaag
      481 cgcagttgcg ggatgcggtg ctcctcgcgc agcgtctctt gcagccgacg gctccacacc
      541 gacaccgcca gcacggcggc gacgcagatc gcgaacagcc agcgccagct caccacggtg
      601 atgaccgcgg cgcccatggt cggcgcgatc accggcacca ggatgaacac ggccatgatc
      661 atcgacatgg cgcgcgacat ctcctcgccc tcgaaggtgt cgcggatcac cgcctgcgtc
      721 accacgcgcg gtcccgcggc gccgatgccc cagaggaagc gcgcggccag cagcgccggc
      781 aacgtcggcg ccagcgcaga ggccagggcg ccgaggccgt agaccgcgta gcccacgccc
      841 agcgtcggct tgcggccgaa gcggtcggcg atcggcccgt acagcaactg cccggcggcg
      901 agccccagga agtaggtcgt gagcacgccg gtgacggcgg tggaggtctc cggcaggccg
      961 aagccctccc ggatggcgcc gaaggccggc agcatcacgt cgatgcccag ggccgccagc
     1021 gccatggaca gagccaggaa ccacgtgagt tcccggcggc ccaacgtgcc ccgcggcggc
     1081 cgggaagtgg acaggagagt cgtcatggtc ccggcataac accgcgctca cggtggatag
     1141 tccgccactg gccggcaatt ggcgcttttc cggtctggcg ggcgtgccgc cctgtccgat
     1201 caggcaggct cgcgggccgc ccatgtggga cgtgggccgc gcgaggtcgc ccgcgtgagc
     1261 gttcggtagc ctcccggcat gtctgacgat caccagcagc cgccggccga cggcgagggc
     1321 gcgcccgggg agtccgcctc tcgcctggac gagctcatcg cggagcggcg cgacaagctc
     1381 gcgcgctggc gcgagcgcgg cgtggagccc tacccggtcg gcgtgcgcgt ggagcactcc
     1441 ctggcggccg tgcgcgagcg ctgggacgcg gcgctgtcgc ccggggagga gaccgaggag
     1501 cgcgtctcca tcggcgggcg cgtcgtcctg cgccgcggcc acggcaagct ggtcttcctg
     1561 gtgctgcgcg agtgggacac cgacctgcag gtgatgtgcc agctcgacgt gctgggcgag
     1621 cccgccatgg ccgacgtcga cgagttggac gtgggcgact gggtggccgt gcacggccgc
     1681 gtgatcgcct cccggcgcgg tgagctgtcc gtgcaggcgg agcaggtgac cctgatcgcc
     1741 aagtcgctgc ggccgcttcc ggacaagtgg cacggggtgc gcgacatcga cacgcgcttc
     1801 cgccagcgct acgcggacct catcgtcaac gccgagtccc gccgcgtgtt cgaggtgcgc
     1861 agccgggtgc tgtccgccct gcgggcggag ctggccgagc gcggctacgt cgaggtcgag
     1921 acgccgatgc tgcacccgat cccgggcggc gcggcggcca ggccgttcgt cacccaccac
     1981 aacgccctgg acctggacct gtatctgcgc atcgcgccgg agctgtacct caagcgcctg
     2041 gtggcgggcg gcatgccgcg ggtgttcgag atcagccgcg tgttccgcaa cgaggggctg
     2101 tcgccgcgcc acaaccccga gttcacgatg ctggagtcct acgaggccta cgccgactac
     2161 accgacgtca tggcgctcac gcaggccctg atccagcggg cggccatgga ggcgctcggc
     2221 cagaccgcgg tggtctacga cggccgcgag gtggacctgg gcggggactg gccgcgctac
     2281 acgctgctcg acctcgtgcg ggaggtcacc ggccgggccg acctgtccta cgacatggac
     2341 ttggccgagc tgcgcaaggt ctgcgacgac tacggcgtgg ccgcggagga gccctggggc
     2401 cccggcaagc tggtgttgga gctgtacgag aagctggtcg agcacacgct gtgggagccg
     2461 accttcgtcg tcgactatcc cgtcgaggtc tcgccgctgg cgcggcgcca ccgcgacgac
     2521 cccttcctga ccgagcgctt cgagttgatc gtcgtcggcc gggagctggc caacgcgttc
     2581 agcgagctca ccgacccgga ggaccagcgc gcccgccttg aggcgcaggc gctcgcgaag
     2641 gccggcgggg acgaggaggc catggtggtt gacgaggact acctgcgcgc catggagtac
     2701 ggcatgccgc cgatgggcgg cctcggcgtc ggcgtggacc gcctggtgat gctgctggcg
     2761 gacgtgcaga cgatccgcga cgtcatcctc ttccccacgc tgcgcccgga gtgatcccgt
     2821 gcgccgcctg cccacgcccg gccaggtggt gctcgcgctc ctgctggcgg gggcggtctt
     2881 gacggcgacc cggcggctgt gggagtagcg gcgctgccgc tcgggttgcg tcacgctacc
     2941 ttggtgggca gcacggggcc gaacgcgggc cggtccccaa cgccgacaac gacgtcggaa
     3001 ggccccgcgc tcaaggccag gacttcgccg ccgagtagtt cctctaacgc cctcgggcgt
     3061 cgttgccggg aatgaaccgg aagggacctc gcgtttgact cgtccctcgt cagaaagact
     3121 gcgggtcccg gagcgtcggc gctccggagt accgggagcg atcacagcgc agcggcggcg
     3181 gcaggaacgg ccgatgatag agttcaagcc caagcacccg agcagggggc agtagatgtt
     3241 cgagcgattc accgatcggg cccggcgcgt ggttgtcctc gcgcaggaag aggcccggat
     3301 gctcaaccac aactacatcg gcacggagca catcctgctc ggcctcatcc acgagggcga
     3361 gggtgtcgcg gcgaaggccc tggaatccct gggcatctcg ctggaaggcg tgcgcgagca
     3421 ggtcgaggag atcatcggcc agggccagac ggcccccgcc ggccacatcc ccttcacgcc
     3481 gcgcgccaag aaggtcctgg agctgtcgct gcgcgaggcg ctgcagctcg gccacaacta
     3541 catcggtacc gagcacatcc tgctggggct catccgcgag ggtgagggcg tggccgcgca
     3601 ggtcctgcag aagctgggcg ccgacctcaa ccgcgtgcgc cagcaggtca tccagctcct
     3661 gagcggctac gccggcggcg aggccggcca gggcgccaag gccggcggcg gcaccgccat
     3721 ggccgaggac cccaagggct cccccgtgct ggaccagttc gggcgcaacc tcacccagct
     3781 cgcccgcgag ggcaagctcg acccggtcat cggccgcacc cgcgagatcg agcgggtcat
     3841 gcaggtgctg agccggcgca ccaagaacaa cccggtgctg atcggcgagc ccggcgtcgg
     3901 caagaccgcc atcgtcgagg gcctggcgca gatgatcgtc aacgcccagg tgccggagac
     3961 gctcaaggac aagcagctct acacgctgga cctcggcgcg ctggtcgccg gctcccgcta
     4021 ccgcggtgac ttcgaggagc gcctgaagaa ggtgctgaag gagatcacca cccgcggcga
     4081 catcgtgctg ttcatcgacg agctgcacac cctggtcggc gccggcgccg ccgagggcgc
     4141 gatcgacgcg gccagcatcc tcaagcccat gctggcgcgc ggcgagctgc agaccatcgg
     4201 cgccaccacc atcgaggagt accgcaagca cctggagaag gacgccgccc tggagcggcg
     4261 cttccagccg atcaaggtgg aggagccgtc cgtggcccac accatcgaga tcctcaaggg
     4321 cctgcgcgac cgctacgagg cgcaccaccg cgtcaccatc accgacgacg cgctggtcgc
     4381 ggccggcaat ctcgccgacc gctacatctc cgaccgcttc ctgccggaca aggccatcga
     4441 cctgatcgac gaggccggct cccgcctgcg catccgccgc ctgaccgccc cgccggacct
     4501 caaggagctg gacgacgagg ccgcgcaggt gcgcaaggcc aaggaagccg cgatcgacga
     4561 gcaggacttc gagaaggccg ccgcgctgcg cgaccaggag aagaagatcc tggagcgcaa
     4621 gtccaaccgc gagcgcgagt ggaagtccga tggcatggac accgtgctca ccctcgacga
     4681 ggaggacatc gcggaggtcc tctccaactg gaccggcatc ccggtgttca agctcaccga
     4741 ggaggagacc gcgaagctgc tgcgcatgga ggacgagctg cacaagcgcg tcatcggcca
     4801 ggaggacgcc atcaaggcgg tctccaagtc gatccggcgc acgcgtgcgg gcctgaagga
     4861 ccccaagcgc ccgtccggct cgttcatctt cctgggcccg tccggcgtcg ggaagacgga
     4921 gctggccaag acgctggcgg agttcctgtt cggcgacgag gactcgctga tccacctcga
     4981 catgtccgag tacatggaga agcacacggt ctcgcgcctc atcggctcgc cccccggcta
     5041 cgtcggctac gaggagggcg gccagctcac cgaggcggtg cgccgaaagc ccttcagcgt
     5101 ggtgctgttc gacgaggtcg agaaggccca cccggacgtg ttcaacgcgc tgctgcagat
     5161 cctggaggac gggcggctga cggacgcgca gggcaagcag gtggacttca agaacaccgt
     5221 gctgatcatg acgtccaacc tgggcacccg gaacctcggc aagcagggcc tgggcttcac
     5281 ggcgcacgag gacgagcgcg acacgtacga gcgcatgaag gagcaggtca acgacgagct
     5341 gaagaagcac ttccgcccgg agttcctgaa ccgcatcgac gagacgatcg tgttccacca
     5401 gctgacgcgc gagcaggtga agtcgatcgt ggacctgatg atcaagcgtg tccgcgaggc
     5461 gctgaagtcc aaggacctcg acatcgagct gaccgaggac gccaaggcct ggctggccga
     5521 caagggctac gacgcccagc tcggcgcccg gccgctgcgc cgcacgatcc agcgtgagat
     5581 cgaggactcg ctgtccgaga agatcctgtt cggcgagttc cgcgccggcc agctgatcgt
     5641 cgccgacgtc gaggacgacg cgatcgtgtt ccgcgcggtg gacagcccgc ccgacgcccc
     5701 gccggtcgag ctggcaggtt ccggcggcaa cgagcagggt tagcaaccgt caagattggc
     5761 tataggtccg cccgagatcg gccgatctag atagacctcg ggagatgtgc cccctccacg
     5821 ggaggcggcc ggaggcgaca agagcctgcg tccgccttgg agcggggccg acccaacccg
     5881 acagtgggcc cggtgcgcca agagcaccgg gcctttgctg ttgcccgaag ccaacaatta
     5941 ctaagttgtc ttagattagt agttgttggt atgttggcca catgaagatg ccgctccggc
     6001 caccggcacc ggctgatgtc ctcagccttg cgctgatgcg gaagctgatg ggccacgtcg
     6061 tcccgctgga cggcgaccgg tatcggcatt gggacacgct gcggcactgc atgcccccgg
     6121 aggggcttac tcctgaggag tggtggatgg ccaccaaact cggccgcgaa ccgctgctcc
     6181 ggtccctgcc gcttcgggac cccgagggga agccgctgag cgtggccttg cccgaccccg
     6241 cgctcgagat gctcatgcgc gtcgaccagc aggccagtgg ccgcatccag gcgccggacg
     6301 cggtgacgaa tcccgccact cgcgaccggt acctcgtcaa ctccttgatg gaggaggcga
     6361 tccgctccag ccagctcgag ggcgcggcga cgacacgtaa ggccgcgaag gagatgctgc
     6421 gctcagggcg ttccccacgc gaccgcagcg agcgcatgat cctcaacaac tacctggcca
     6481 tgcagttcgt gcgggagcac gtgaccgagc cgctcactcc cgagctggtc ctccaactcc
     6541 accgcatcgt cacggacggc acgctggagg accccgccga cgccggacgg ctccaacgtc
     6601 ccggcgagga gcgcatcaag atctggtggg acggtgaact cgtctacgaa ccgccgcccg
     6661 cggagcaact ggaggagcgc ctccggctgc tgtgcgactt cgccaatggt ggagcgatcg
     6721 acgggttcct gcaccccgtc gcccgggcgg tcctcgtcca cttctggctg gcctacgatc
     6781 acccgttcgt ggacggcaac ggccggacgg ctcgcgcgct gttctactgg gcgatgctgt
     6841 cgcaggggta ctggctggcg gagttcctgc ccatctccaa catcatccgc caggcgcccg
     6901 cgcgctacgc caagtcattc ctgtacgtcg agaccgacga gggcgacacc acctacttcc
     6961 tcctgtcgca gctgcgcatc ctttgccggg ccatcgacga gctgcacgcc tacctcgggc
     7021 gcaagacgcg ccaggtccag gaggtcgagg tgctcctgcg gcgctcgccc gggttcaacc
     7081 accgccagat cgcgctgctc tcccacgcgc tgagggacgc aggcgcgcag tacacattcg
     7141 cgacgcacca gcgcagtcac ggcgtcgtgt accagtcggc gcgcaccgac ctcctcgacc
     7201 tggccgagcg gggactgctt cagcagacca agcgcgggcg ggcctacgtc ttcttcccgg
     7261 ctgcggacct cgaacggcgt ctgaaggcgt tgtccggcgc cgagtcgtcc tgacacgggg
     7321 ttagtccatg cgggagctca ggcgccagca ccacagcgcc accgcgacgc cgaagagcag
     7381 cgtggcgttc acgaacaggt agccgccggt gtcgaggtgg ccctggtcga tcaggtggat
     7441 caccaggtgc ggcacgctga acccgagcgt ggcgatcaac gcggcccgca ccgcatggcg
     7501 gtgcagcacc accgccgcat acaccagggc tgcgccgaag gcgaggtagc cgccgccgat
     7561 gtcctggacg aagtgctcgt tgtagacgcc gaaggccgac ggtgcggccg gaccgccgaa
     7621 gtcggcgtac caggacgcag gcccgaagat cccccacacc gcggacacca cggccggcac
     7681 ggccaggatg atcagccaca cccgcagttg ccttttggtg cccgatcccg ccgctgcgga
     7741 agcgcctcgg cgcgtcgtct cgtcggtgct catcgtggtt ccttcgcccg ccggcgcggt
     7801 gaccgagcag tccccgcgtc cggcgagtgt gcgggacgcg gtcaggcggg acaaggaccg
     7861 gcgaccgcgt gacgacgccg gtcgcgaggt ccagcagcag ggcggtcttg tgtcgctcag
     7921 ggacgagttg tgtccgtgtc cggccacaac cggtcccgga aggcgggtag cctcgggcca
     7981 tgcgcctggt catcgcccgc tgctccgtgg actacgcggg ccgcctcacc tcccacctgc
     8041 ccagcgccgt ccggctgctc atggtcaagg ccgacggctg cgtcgccgtg cacgccgacg
     8101 gcggcgcgta caagccgctg aactggatga acgcgcccaa cacggtgacc gaggaggagg
     8161 gtcgctgggt cgtcgccaac cccaagggcg agacgctcac catcacgctg gaggaggtcc
     8221 tgcacgactc gctgcacgac ctcggcgagg acccgggcct gtccaaggac ggcgtcgagg
     8281 cgcacctgca ggagctgctg gccgagcacc ccgacgagct gcaggacggc ctgacgctgg
     8341 tgcgccgcga gttccccacc gacatcggcc ccgtggacct gctgtgccgc gacggagacg
     8401 gccaggccgt ggccgtggag gtcaagcgcc gcggcgagat cgacggcgtc gagcagctca
     8461 cgcgctacct ggagcgcctc aaccgcgacc cgatgctcgc gccggtgcgc ggcatcttcg
     8521 ccgcccaggt gatcaagccg caggcgcgcg tgctggccga ggagcggggc atctactgcg
     8581 ccgaggtcga ctacgacgcg ctgcgcggca tcgagtccga cgccctcaag ctgttctagc
     8641 cacggcgtag cgcaggtaca gctcgccggc gtcctccaag gcgtgggtca gcgtgagtgg
     8701 ccgcgcggcg gacagctcgc cgaccaggct cgccgcctgc gcaccgacca tgaccggcgc
     8761 caccgtcagg cacagctcgt cggccagccc ggcgtcgaac agcggtgcgt tcagcgtcgg
     8821 cccgccctcg cacagcaggt gcgccatgcc gaagcgctcg cgcagcgcgg cgatccccag
     8881 ggcaaggtcc acctcgtcct cgccggcgat cagcaggacg cccgcgcggg ccgccgcgtt
     8941 gcgcccgtcc tccggggcgg cctcgcaggt cagcaccacg gtgggcgcct tggcctcggt
     9001 gaacagcggc gcgccgtagt cgaggtccag cgaccggctc accaccacga tctgcgccgg
     9061 caacggccgc ccgtcggcga tgcgccgctc gtgcagctcc tcgcgcagcc ggtgtggccc
     9121 gtagccctcg gcacggaccg tgcccgctcc caccaggacg ccgtccgcca gcgcgcgcag
     9181 cgtgcggaag accatctggt cgccggctcc gcccagcgcg gccgtgcgcc cgtcgcggtc
     9241 ctgcgcgcgc ccgtcggcgg acagcaccat gttcacgcgc aggtgcgccg cgccgtcgtc
     9301 cacgcggtag gcgtcgtaga cgtccacgtc gtcggcgggg tccggaagca ggcggcgcat
     9361 ggccgcacgc tacccggttg cgccgcgcgt aggccgctgg cacgctcgcc gccatgcccc
     9421 actcgcctcc ccgcgcccgt gcgctgcgct tccagcggct gctgctggac tggtacgccg
     9481 cccaggcgcg cgccctgccg tggcgtcgca cgcgcgaccc ctaccgggtc ctggtgagcg
     9541 agatcatgct ccagcagacg caggtgtccc gcgtggagcc gctgtaccgg gcgttcctgc
     9601 gccggttccc gtcggcgcgt gcgctggccg ccgccccggc ggccgaggtc ctcggcgcct
     9661 ggcgcggcct gggctacaac cgccgcgcgc tgaacctgca ccgcgccgcg caggcggtgg
     9721 tgcagcgcca cggcggtcgc gtgcccggtg acctggaggc gctgcgggcg ctgcccggtg
     9781 tcggcgacta cacggcgcgc gccgtgctgg ccttcgcgtt cgggcgggac gccgcgccgg
     9841 tggacaccaa tgtcgcgcgc gtgctcgccc gcgccgtcgc cggcgcgccg ctgggcaagg
     9901 cccaggcgca gcgcctcgcc gacgccgtgc tgccacgggg ccgcgccgcc gagtggggca
     9961 acgccctcat ggacctcggc gcccgcgtct gcacggcacg cctcccgcgc tgcgaggact
    10021 gcccggtcgc ctcggcgtgc gcgtggcggc gcgccggtgg tgccgacccc gccgccgcgg
    10081 gcgccctgcg gccccgcccg caggcgccgt tcgccgggtc cgaccgcttc caccgcggca
    10141 ggctcgtcga cgcgctgcgc gacgggccgc ttcttcccgg cgcgcttggc cgcgccgcgc
    10201 agctcgacga cgccgccagg ctggagggca tcgtcgcggc gctggtgcgc gagggcatgg
    10261 cccagtgggc cggcggcgtc ctgcgcctgc ctcgggcgga ggccgcgcca cgcgcctagg
    10321 ctcaccgccc aggcggggac agtctcccgc gcaggtgtag gcgggcgcac cgacggggca
    10381 ggcaaggacc catgacgatg gacgcggcga cgctcaagcg gcttggcaag caggtgggcg
    10441 cggaggtcaa ggcggacaac gtcggcctgc tggcctccgc cgtggcgttt cgcatcttcc
    10501 tgtcgatctt tcccagcctg atcgccgtgg tgggcgtgtt cagcctggtg acctcgccga
    10561 gcgacatcac cgggctgatc aacgaggcgc ggggcgtcct gccgtcggag gcgcagggct
    10621 tcctggagcg caccctcacc cgcatggtcg ccgagggcag cggcggcggc tttgccatcg
    10681 ccggtgtgct cggcgggctc tgggcggcca gctccgccgc cgccgtgctg atcaaggcgc
    10741 tcaacgccgc ctacgaggtg gccgagtcac gagggttcgt gaagcagcgc ctcgtcgcgc
    10801 tgctgctcac cggcgtgctg ctgctcgcca ttttcggcct gctggcgctc ctggtggtcg
    10861 ggcgcttcct gcaggcgtgg ctggtgccgg actcgctgca ggggcccgtg gtggacaccg
    10921 tgttcctgct gggccgcgtg gccggcgccg tgctgctgat gatggtgctg ttcgccgtgg
    10981 tgtaccgggt ggggcccaac cgcggccgcg cggcgccgtt cgagtgggtg acgcccggcg
    11041 cggtgctggc cgtgctggcc tggctggccc tgtcggcgct gttcgccctg tacacgcgcg
    11101 cgttcggcag ctacggcgcc acgtacggct cgctggccgg cgcggtggtg atgatgttgt
    11161 ggctgcagct caccatgacg acgctgctgg tcggcgccga gctcaaccag gtgtggcggc
    11221 gccacacgcg cgcgcgggac ggcgcgggct cccgcgtcga gccggattga gcggcggcgt
    11281 cacaccccgc cactagcatc cgcgccatgg cgaagacgag gtcgcaggcc cgctgcggcg
    11341 aatgcgggca tgtcgaggcg aagtggatgg ggcgctgccc cgcctgcggc gcgtggggct
    11401 ccatggcgga ggaggcccgc ccggccgcgt cgtcggctgc ggccgggcgc gccgcggcgc
    11461 ccgtgcgcgc cgccgtgccg atcgcgcagg tgccgctgga cggcgaccgc cgcgcggcca
    11521 cgggcatcgg ggagctggac cgcgtgctgg gcggcgggct ggtgcccggc tccgtgaccc
    11581 tggtcggcgg cgagccgggc gccggcaagt ccacgctgct gctccaggcc gcggacgcgc
    11641 tggcgcgccg cggcgcggcg gtgctgtacg tctcggcgga ggagtccccg tcgcaggtgc
    11701 gcatgcgcgc cgagcggctg ggcgcgctgt cggagcgcct gctgctgggc agcgagacgg
    11761 acctggccgc ggtgctcggc ctgctggacc tgcacaagcc cgacgtgctg gtgctggact
    11821 ccatccagac cgtcaccgac gccgacacgg ccggcaccgc gggcggggtg tcgcaggtgc
    11881 gcgcctgcgc cggcgcgatc gtgcgggcgt gcaaggagtc gggcatcgcg ggcgtgctgg
    11941 tgggccacgt caccaaggag ggccagctcg ccgggccgcg cacgctggag cacgtggtcg
    12001 acaccgtgtg cgagctgtcc ggcgaccgcc accacgcgct gcggctgctg caggcggtca
    12061 agcaccgcta cggcgcggtg ggcgaggtcg gctgcttcac catgaccggg cgcggcatgg
    12121 tgggcgtgga cgaggccggg cgcctgttcg tcggcgaggc gccggacggc accacgggcg
    12181 tggcggtgac gctggccctg gagggccgcc gcccactggc gtgcgaggtg caggcgctca
    12241 ctgtgccgac cacggccgcc aacccccggc gggtcgccag cggcctggac ccgcagcggc
    12301 tgtcgctgct gctggcggtg ctggaggcgc gcgcggaggt gaagctgtcc accgacgagg
    12361 tgttcgcctc cagcgtgggc ggcgtgcggc tgtcggagcc ggccgtggac ctggcgctgt
    12421 gcctggccgt ggcgtcggcc aagaagtcca agccggtgcc gcgccgctgg gtggtgctcg
    12481 gcgaggtcgg cctggccggc gagctgcgcc tggtcgcgca gaccgagcgc aggctggcgg
    12541 aggcggcgcg cctgggcttc cgcggggcga tcctgccgga ggcatatgat ggcgacgcct
    12601 tcgggctgcg gctgcacaag gcgccggact tgggcggcgc gctggccgtg ggcctcgccg
    12661 cacactgagc cgcccgcgaa aggagtcccc ctccgtggcc ccgcgtgacg accgcctcct
    12721 ggccgtcctg cagaaggttg ccccgggcac ggcgctgcgc gaggggatcg accgcatcat
    12781 ccgcgcgggc aagggcgcga tcatcgtcct gggcgacggc gaggagatcg accgcatcgt
    12841 ctccggcggc ttccgcctgg aggccaagtt cacggcccag cgcctgtccg aggtcgccaa
    12901 gatggacggc gcgatcatcc tggacaacac cgtggagcgc atcctctacg ccaacgtgca
    12961 cctggtgccg gacccgtcca tccccaccgc cgagaccggc acgcgccacc gcaccgccga
    13021 gcgcgtcgcc aagcagaccg cgcggccggt gatctccgtc agcgagtcga tgcgcatcgt
    13081 gtcgctgtac gtggacgcgc agaagcacac gctggaggag atcagttcca tcctgttccg
    13141 cgccaaccag gcgctggcca cgctggagcg ctaccgcatg cgcttcgacg aggtcaccgc
    13201 cggcctgtcg gcgctggaga tcgagaacgt cgccacggtc cgcgacgtgg tcagcgtcct
    13261 gcagcgcgcg gagatggtgc ggcgcatcgc cgaggagatc gacgcctaca tcgccgagct
    13321 gggcaccgac gggcggctgc tgcaactgca gctggacgag ctgctggccg gcatcgaggg
    13381 cgagcgcatg ctgatcgtgc gcgactacat gcccgatcgg cgccgcaagg tcgagtcggt
    13441 gctgggggac ctgcacgcca tcccgcagca ggacctgctg gaactggcgc gcgtcgccga
    13501 cgccgtgggc ctgggcaccg gcgagggcaa gctggacgag ccgatctcgc ctcgcggcta
    13561 ccgcctgctg tcgcgcatcc cgcgcctgcc ggaccgcatg gtggaccgcc tggtgtcccg
    13621 gttctcctcg ctgcagcgcc tcatggaggc cacactggac gacctggacg aggtcgaggg
    13681 catcggcgag gcccgcgcgc ggtcgatcaa ggaggggctg gcgcgcctgg cggagtcctc
    13741 catcttggag cgctacgcct agcacgcggc gggcgcccgg tgcgtccccc attcggggga
    13801 catgcgcgga aatggcctct gacctgggga tttgcggttt gggccacccc ttgggtgtgg
    13861 taggatcgat cagtacgtgc cgatcaagcg ccgcccggcc cccccgctgg gtgcggtgaa
    13921 gggatacgac cgtatgacgt accgggttgg agacaccgtc gtctacccgc accacggtgc
    13981 ggccgtcatc gagaagcgcg agaagcgcac gctggccggc caagagcgcg actacctggt
    14041 gctccgcctc acctacggcg acctgacctt gatggttccc gccgacgcca ccgaagaggt
    14101 gggcctgcgc agcgtggtga ccaagaagga agtcgacgag gtcctgaagg tcctcaagaa
    14161 gcgcgacggc aacatgccga ccaactggtc gcgccgcttc aaggccaact acgagaagct
    14221 gcgctccggc gacatctacc aggtcgccga ggtggtgcgg aacctggcgc accgggaggc
    14281 cgagaagggc ctgtccgccg gtgagaagcg catgctcacc aagtccaagc agatcctcgt
    14341 gtccgagctg tccgcagcgc tgaagaagtc ggacgaggac accgaggtgc tcatcgacga
    14401 ggtgctcggc acctgagcgg gcagtcccgg cgacgagggg ccgcgagcac tccggtcctc
    14461 gtcgagctga tccgcctggc ggtggtggtc gcggccatcg gcggcgccta cgcgctcggc
    14521 gagtgggccg cgttcgagct cccgcccgaa ggcgtcctgg gcagcgactg gttccccgtc
    14581 gtcgtgctcg tgcttgccgc gtccgtggcc tacctcgtcg gcggagcggt cggccgcgcc
    14641 gtgcacggtg gcgtggacct cgccgagcgg cggctgcgcc gggttccgac cggagagctg
    14701 ctggccggcg cgatcggcgc cgtggccggc ttcctgctga ccctggccgt ggtctggccg
    14761 gtcctgctgt tcgagggccg cgtggtgacc gtgccgctgg ccgtcctggt cgcggtggtg
    14821 ctcacctcgg ccggcgcgcg catcggccgc gcgcgcggcg gcgacttcct gcgcttcatg
    14881 ggcgtgccgg ggcgcctgcg cgtctccagc cccgcgcacg gcggcggcgt gaagctggtg
    14941 gacagctccg cgctggtgga cggccggctg ctcgacgtct gccgcgcggg cttcgtggag
    15001 ggcaccctgg tggtgccgcg cttcgtgctc gcggaggtgc agggcctggc cgacgccggc
    15061 gacgagcagc ggcgcacgcg cggcaagcgg gcgctggacg tgctggccgc gctgcagcgt
    15121 tccgcgggtg tcgccctgga ggtccccgac gacgactacc ccgaggtcgc ggacgtggac
    15181 gccaagctgc tggcgatggc gcgcgacatc gacgcggcgc tggtcacggt ggacggcaac
    15241 ctggggcgcg tggccgaggt gcagggcatc cgtgtgctga acctgcacac gctggccgag
    15301 acgctgcgcc cgccggtgct ggtgggcgac accgtgcgcc tgcggctgac gcggccgggc
    15361 agggagcccg gccagggcgt cgggcatctc gacgacggca ccatggtggt ggtcgagggc
    15421 ggcgccgccc acgtcgagga cgacgtgcag gcggagatca ccagcgtgct gtccaccgcc
    15481 aacggccgga tgctgttcgc ccgcctgggc gaggaccggt gacgaccacc gccgcggtgc
    15541 tggtcgccgg gggcgccggg gagcgcctgg gactcccgca gcccaaggcg ttcgtcccac
    15601 tcgcgggcga gccgatgctg gcgcgcgcgg cgcgcgcgct gtcggccgcc ggcgtgttcg
    15661 cccgcatcgt cgtggtggtg ccggagggct ggcaggagcg cgcgcgcgag ctctgcgcgg
    15721 acgtggacgc cgagctcacg gtctgtgccg gcgggcgcac ccggcaggag tccgtggcgc
    15781 gcggcgtggc gcacgccgcc gaggatgtcg tggcggtgca cgacgcggcg cgcgccctgg
    15841 tgccgccgga gttggtgcgc gacgtcgtgg ccgccctcac cgaccagtgg gacgccgtcg
    15901 cgccggcggt gccgatcgtg gacacggtca agcgcgtgga ccccgacgac ggccgcgtgc
    15961 tggagaccgt gggccgcgcg acactgcgcg ccgtccagac gccgcaggtg ttccgccgcg
    16021 acctgctgac gcgcctgcac ggcgaggcgc acggcgccga cgccaccgac gacctgctgc
    16081 tggtggagca ggccggcggg cgggtgcgcc tgctcgacgg cgacgtgcgc aatctgaagg
    16141 tcaccacggc ggccgacctg ctgttcgccg aggcgctgct gcgcgaggat gcccgggcat
    16201 gaggatcggc cagggcgtgg acgcgcaccg cttcgcggag gagggggcgg gccgtccgtt
    16261 gcggctgtgc ggcgtcgaga tccccgacgc cccggggctg gaggggcaca gcgacgccga
    16321 cgtggcgctg cacgccgtcg ccgacgccat cctgggtgcc gccgccctgg gcgacctggg
    16381 agcgctgttc ggcacgaacg acgagcgcta cgcgggcgcg gactccgcgg tgttcctgca
    16441 cgacgcggtg caccgcgccg cccagttcgg cctgcggatc ggcaacgtcg actgcaccct
    16501 cgtggcgcag cgtccccgcc tgtcggctca cctgccggcc atgcgcgagc ggctcgccga
    16561 gctgctgggc gccgacccgg ccgcggtgtc ggtcaaggcc accaccacgg acggcctggg
    16621 cttcaccggc cgcagcgagg gcatcgcctg cctcgccgtg gtcctgctcg ggccggtcga
    16681 cccggctcag gccacggccc accccacagc caggtagcgg ccgggccgtc ggctaggctc
    16741 ttgcccctat gcgcctgacg aacacgctca cgaaccgcga ggaggagttc acacccctcc
    16801 gcgacgacct cgtgaccatg tacgtctgcg gccccaccgt gcagggcccg ccgcacttcg
    16861 gccacgcccg cgcggcgatc gtgccggacg tgctgcgccg ctacctggag tggagcggcc
    16921 accgcgtctt ccacgtccgc aacatcaccg acgtcgacga caagatcatc gcccgcgccc
    16981 aggaggaggg ccgcgacttc ttcgaggtcg gcgagcactt cgcgcgcgtg tgggaccagc
    17041 agatcacccg gctcaacgtg ctgtggcccc atgtcgtgcc gcgcgccacc ggccacatcc
    17101 ccgagatgat cgcgctgatc acgcggctga tcgccaacgg cgccgcctac ccctccggcg
    17161 gcgacgtgta cttccgcgtc cgcgccttcc ccgagtacgg caagctgtcc aaccgcaacg
    17221 tcgacgaact gcgcgccggt gcgcgcatcg agccgggcga gcacaaggag gaccccgccg
    17281 acttcgcgct gtggaaagcc gcgaagcccg gcgagccgag ctgggactcg ccgtggggac
    17341 cgggccggcc cggctggcac atcgagtgct cggcgatgtc cagcaagtac ctgggcgaga
    17401 ccttcgacat ccacgccggc ggcgtcgacc tggtgttccc ccaccacgag aacgaggtgg
    17461 cgcagtccga ggcggcgacc ggccggcagt tcgcgcgcta ctgggtgcac aacggcctgc
    17521 tgacgctggg cgcggagaag atgtccaagt ccgtgggcaa catcatctcg ctggacgagg
    17581 cgatcgccac ctacggcggc gacgtgctgc gcttcttcta cctggcggcg cactaccgct
    17641 cgccggtgga gttcagcgag gagcgcctga gcgaggcgca ggcggcgatc gagcgctggc
    17701 gcacgttcct gcggctggtc ccggagggcg acggcgctgc cgtggacacg cccggaggcg
    17761 acgagccggg cacgtaccgc gaccgcttcc gcgccgcgct ggacgacgac ctgtccaccc
    17821 cgcaggccca cgcggtgctg ttcgacctcg ccaccgccgg gttggcgcac gccgaggcgg
    17881 gccggagcca ggaggcgtcc gcgctgcgcg ccctgttcgt ggaactggcg cgcgacgtga
    17941 tgggctaccg gctggaggac cgggccgccg gcgacctggt cggcccgctg gtggaggagc
    18001 tgctggcgct gcgccaggag gcccgcgccc gcaaggactt cgccacctcg gatcgcgtcc
    18061 gcgaccggct ggcgggcctc ggtgtggtca tcgaggacac gccgggcggc tcccgctggc
    18121 acctcgcctg atggcggacc gcccgcggcg cgaccgccgg cccacgaccg ccaagccggg
    18181 ccgcgccgac gccgggcgcg gcaagccggc gaagcgcgag gccccggcgc ctcggcgacc
    18241 gggtggtgcc ccggcacggc gtgcgcgagg tgctggtcga gcgcctggac ggcaacgagg
    18301 agatcctcga gctggcgcgc gggcgcaagg tccccgtgcg ccgcgtggcc agggaggagc
    18361 tcgacgcggc cgcgcagggc gtgctgcacc agggcgtggt ggcggtcgcg ccgccgttcg
    18421 cctacgtcga cctgcaccag gccgccacgg gcgacctcgt ggtggtgctc gacggcgtca
    18481 ccgaccccca gaacctgggc gcgatcgccc gcagcgccga ggccgccggt gcgcgcggcc
    18541 tggtccttcc caagcgccgc agcgcccatg tgaccgccgc ggcggagaag gcggcggcgg
    18601 gcgcgctgtc gtggctgccc gtggcgctgg tgcccaacat cgtgcgggcg ctggaggaac
    18661 tggccgagcg tggcttctgg tcggtgggcc tggccggcga ggccggttcg gcgctgtggg
    18721 atagcgggct gctggacggg aaggtggcgc tggtcgttgg cgcggagggc ggcggcctgt
    18781 cccgcctggt ccaggagcgg gtcgacgagc ggctgcagat cccgatgacc gggcacgccg
    18841 aatccctcaa cgcgtcggcg gccgcggcga tcgctctgtt cgagattgca cgtcggcgtc
    18901 accagagaac ggacatatag gaccgctttt tggggttgcc acaagaaatg gcggcccaac
    18961 ccctccgcat cgtctattga ccggccccgt acttacccgc tttactcctg gtaacctcgc
    19021 ccgggtcccg gcgcgccgca aacccccgcc ggctgcgccg cccccacgtc tcgaaaggta
    19081 cgggttgagc ctggagatcg tcatcgacct caccgaggcg cgcacggagg acgacgacgt
    19141 gctggtggct cgggcgtgga acggggacga gtctgcgctg ggcacgctcc tggatcgcta
    19201 ccggggcttc gtccgcggca aggcgcgctc ctacttcctg gccggcgccg accgcgagga
    19261 cgtggtgcag gaaggcatga tcggcctgta caaggccatc cgcgactacg acccggcgcg
    19321 ccagtcgacc ttccgggcgt tcgcggacct gtgcgtcacc cgccagatca tcaccgcggt
    19381 gaagacggcc acgcggcaga agcacggccc gctgaactcc tacatctcct tctcgctgcc
    19441 cgcccacggc gacgacagcg accaggagct ggtcgactgc ctgccggcgg ccgcggagga
    19501 cccgtgcgag cgcgtggtgg ccggccacga cctggccgag ctcgagcgct ggttcggcga
    19561 gatcctgtcc gacctcgagg ccgaggtgct gcagcgctat gtcagcggcg agagctacgc
    19621 cgagatcgcc gcggcgctgg accggcacgt caagtccatc gacaacgcgc tgcagcgcat
    19681 caagcgcaag ctggacctgt acctgcaggc gcgcagcttc gagcccgccg tctagcaccg
    19741 cctcgagccg ccgcccagcg cccgcgacgg tctaggctgc ccccctgagt ttccgctcac
    19801 agtgagtggt cgctccggta ttccggagaa actgcacacg ctgcgcctgg agccggcaat
    19861 ggtcgagtcc ctcagcgtcg acgtcgccgt ggtgggtgcg ggcctgtcgg ggctcgtcgc
    19921 cgcgcgggac ctcgccgcgg ccgggagggg cgtcgtggtc ctggaggccc gcgaccgcgt
    19981 gggcggccgc gtgctcaacg agccgctgga cgagcacggg cagatcgtcg aggtgggcgg
    20041 ccagtggatc gggcccggcc aggaccgtgt cgccgagctc gccgccgacc tggggctgga
    20101 gacgttcccc acgcac
//
LOCUS       DATMKC010000269         8467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_120421, whole genome shotgun sequence.
ACCESSION   DATMKC010000269 DATMKC010000000
VERSION     DATMKC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120421"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VNU01_07935"
     CDS             <1..1340
                     /locus_tag="VNU01_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWH32587.1"
                     /translation="AAHSDNRAGRERLPSRWLLAEAGRLAGRVLHSEDLPRLRDQAWL
                     DWVGSSAPAAGAHAAGAAELLIASLARWRAHGLALAEHPLTQGTLALGLEALRARRGG
                     EFGRWTGHVGALPGLALGDTPLSPTRLQTWAACPFRSYLERVLGVEELLRPEETVRIE
                     PREWGNLLHRVLERFVAEGEPPAPGAPWTPADRARLHRLGDEEADRWEQAGATGKALL
                     WRLDRARLHRTLDAFADSDQALRARDGTAPAHTERGFGYDGDPVTLALGDGRTVAFRG
                     RVDRVDVGAAGDVVVIDYKTGAAASRYRAALAAHPVAGGQLLQLPVYALAAGEAARPS
                     AGGVRAAYWFLGEDGVDEIAWELDDDGAAAFGEAVATIADGMEQGLYPQRVGHEWARD
                     RETCASCAYELVCDRDRERRWPIVRALPLLSGLARLDGGAAAGPGTATGGGRD"
     gene            1333..4647
                     /locus_tag="VNU01_07940"
     CDS             1333..4647
                     /locus_tag="VNU01_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWH32588.1"
                     /translation="MTDVAPPDAASRERVLTELGTTLFVEAGAGTGKTTALVGRILSL
                     VATGEAQLREVAAITFTEAAAAQLRDRVRAAVEHAAGDTGRPAEQRERMAVAATQVDE
                     AAILTLHGFAQAVLAEHPIEAGLPPEFEIVDELRAAELLDEAWAELQEDLLSDPGLRA
                     PMETALTLGLTLYSLERLARAAAEAYDRLAEHPPAGAPPGRAPAEDLADRIAATARWR
                     ARNLRPDALLDHLDDLAELARALRAAPGELEGLRLLAGRKLSCSRGQKGDWDGGATDI
                     KHGLADLERQRRDVLAHAGRTALAPILERVRAWALAQARRRRTQGQLTFHDLLVAARD
                     LLRDHAETRGLAASRWRRLLIDEFQDTDPLQVDIALALAETDPRPGRLFFVGDPKQSI
                     YRFRRADIAVYGAARERFADGLVPLVANFRTVPGVVAWVNAVFAGLMDGEPGQAPFAP
                     LLAMRPALRDGPAVHVVGGPCEKLPAAEAREREATAVARAVRAVRDEGWPVHDAHDKV
                     TRPARYADIALLLPARTALPAVERALEDAGVPYRVEARSLVYESQEARELLALLRAAD
                     DPTDEVAVVAALRGPALACPDDALAQWAAAGGGWDYRQAAPAGAPDIVAEAMRRLAAC
                     HAARFAADPAALVDLLVRELRLLELGAGRPRPRDAWRRLRYVIDEARALAERGGSLRE
                     LVRRIERQAADQVRVTEQVLPDEDDDAVRILTMHAAKGLEFGVTVLCGLAGAGGGGGD
                     PLLWDAGGQGQVRLGDHLETQGYAEAKEAERLAQRQEHHRLLYVAATRARDHLILSLA
                     HTPPKASPAGREPQGAELLWERSAGCAQLWRRWEPPEPVDVRDPAEGAAAPAGPADAA
                     GLAAAERAAAQAWLDKRGAALSSRARLPVGAATALTAQDRPEHSGHDPADDSGEHPQA
                     DEHAALPPFRRGRAGTAIGRAVHAVLQTIPFDAAAALVAAAARAQADAEGIPGRAGEV
                     AALVASALRSAPVRAASAGRSWRELPVAAPVGGLLLEGYVDLLYEQDGELVLVDYKSD
                     RVDDASLAATTERYATQLGAYALALEAVLSRRVARAALVFVREGEARVAWVDDLAAAA
                     ARARELVAAHAAPPSG"
     gene            4698..5609
                     /locus_tag="VNU01_07945"
     CDS             4698..5609
                     /locus_tag="VNU01_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HWH32589.1"
                     /translation="MPPAPPLRLHPEVADALAHGTAVVALESTLIAHGLPRPGNLDAA
                     RSLESIVRAAGAVPATVAVLGGVAHVGLDDDALTRVAHDDLRKLAVRDLPLALGSGAD
                     GATTVSATATLAALAGIRVFATGGLGGVHREAAQTWDESADLPALASTPVAVVCSGVK
                     SILDIPATLERLETLGVPVVGYRTARFPGFYLSDSGQDLDWRADSPEEVAAMLRAEDA
                     LGGGRGLVVANPVPAGEQLDPRLHDDVLRAGLDALAAQGVRGKDVTPFLLSWMHEHTG
                     GESLRVNVRLVEHNARLAARIAVAYAS"
     gene            5606..6520
                     /locus_tag="VNU01_07950"
     CDS             5606..6520
                     /locus_tag="VNU01_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWH32590.1"
                     /translation="MKVLVVGDLVVDVVAAPKGSVAVDSDTAARVALRGGGSAANAAA
                     WLALAGAQVALVARIGDDMAGRLLREELSAGRVEPLLAVDASAATGTIVVLVEPDGRR
                     TMLTDRGASALLAPRDLPAERFTAPAHLHLSGYTLLGQASRAAGLHALRLAAAAGMTS
                     SVTPGTAEPLRQAGAESFLSWTAGATCCLANREEAGVLTGAGPGAPHAELALALAERY
                     AEAVVTAGADGAAWASGTEVVALPAVDAAAVDTTGAGDAFAAGLLAAWLGGASREDAL
                     RAGLRLAAQAVALAGGRPPAAVSDMGLG"
     gene            6560..7294
                     /locus_tag="VNU01_07955"
     CDS             6560..7294
                     /locus_tag="VNU01_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH32591.1"
                     /translation="MERATDDTRPRTAFVAGGGGALGQAVVARLLDAGERVCVAWVVE
                     DEAAALRARFGDQADSGRLRLSRCDVADGDQVAALLDVLEADWGPLWLACSVVGGWAG
                     GQTVEELDDLSVLDRMLLLNLRTATAVAREGLRHMGPAGGRVVLTASRTALVPAPSQA
                     AYSAAKAAVLSLVATLAEELRGSGRTANAIVPKVIDTPGNRQAMPDADHSRWVPPEAI
                     AEVVAWLGSAASWPVSGAAVPVYGDA"
     gene            7296..7829
                     /locus_tag="VNU01_07960"
     CDS             7296..7829
                     /locus_tag="VNU01_07960"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HWH32592.1"
                     /translation="MRRVTVRPALLLAALLLAGAACADEPRRSATDGPGEAPGACEPV
                     ERPPEQSGGHLLGEATPPVPYSSTPPTSGWHASGNPARGVHGAGDPLSEPEQVTILEL
                     GGVVLSWNGLDGDARERLESYAREHEDTVASTPYGALAPGEVAMTAWGVLQRCDGVDL
                     EAVDAFVAEHAGGGPDH"
     gene            7912..>8467
                     /locus_tag="VNU01_07965"
     CDS             7912..>8467
                     /locus_tag="VNU01_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWH32593.1"
                     /translation="MRRIPTLLCLALLAAACGDAPSGQDRPLVAATPWPLQWLAEQVA
                     PEAEVRGLGQAGQDPHELDLRPAERELLERADVVLFVGPVGFQPQVEEAAAGRGEAAV
                     AITQAAADLLLDAEAHEHAEDEHAEDEHAEDEAVDPHVWFDPRTLAEAADALAEALAA
                     VDPDRADGYRERAAAVRGRLLALHE"
BASE COUNT          918 a         3232 c         3379 g          938 t
ORIGIN      
        1 gcgctgcgca cagcgacaac cgggccggcc gggagcggct gccctcgcgc tggctgctgg
       61 ccgaggcggg acggctggcc ggccgcgtcc tgcacagcga ggacctgccg cgcctgcgcg
      121 accaggcctg gctggactgg gtcggcagct ccgcgcccgc ggccggggcg cacgccgccg
      181 gggcggccga gctgctcatc gcctcgctgg cacggtggcg cgcccacggg ctcgccctgg
      241 ccgagcaccc gctgacccag ggcacgctgg cgctgggcct ggaggcgctg cgcgcccggc
      301 gcggcggcga gttcggccgc tggaccggcc acgtcggcgc gctgccgggc ctggcgctcg
      361 gcgacacgcc gctgtcgccc acacggctgc agacctgggc ggcctgcccg ttccgctcct
      421 acctggaacg cgtgctcggc gtggaggagc tgctgcgccc ggaggagacc gtgcgcatcg
      481 agccgcgcga gtggggaaac ctgctgcacc gcgtgctgga gcgcttcgtg gccgaggggg
      541 agccgccggc gcccggggcg ccgtggacgc cggcggaccg cgcgcggctg caccgtctgg
      601 gcgacgagga ggctgaccgc tgggagcagg ccggcgcgac cggcaaggcg ctgctgtggc
      661 ggctggaccg tgcgcgcctg caccgcacgc tggacgcctt cgccgactcc gaccaggcac
      721 tgcgcgcacg cgacggcacc gccccggccc acaccgagcg cgggttcggc tacgacggcg
      781 acccggtcac cctggcgctg ggcgacggtc ggacggtggc gttccgcggc cgcgtggacc
      841 gcgtcgacgt gggcgcggcg ggcgacgtcg tggtgatcga ctacaagacc ggcgccgccg
      901 cgtcccgcta ccgggccgcg ctggcggcgc accccgtcgc gggcggtcag ctcctgcagc
      961 tccccgtgta cgcgctggcg gccggcgagg cggccaggcc gtccgccggg ggcgtgcgcg
     1021 ccgcgtactg gttcctgggc gaggacggcg tcgacgagat cgcgtgggaa ctggacgacg
     1081 acggtgccgc cgcgttcggc gaggccgtgg cgaccatcgc cgacggcatg gagcagggcc
     1141 tgtacccgca gcgcgtcggc cacgagtggg cgcgggaccg cgagacctgc gcgagctgcg
     1201 cctacgagct ggtgtgcgac cgggaccgcg aacggcgctg gccgatcgtc cgcgcgctgc
     1261 cgctgctcag cgggctggcg cgcctggacg gcggcgccgc cgccggcccg ggcaccgcga
     1321 cgggaggcgg ccgtgactga cgtggcgccc ccggacgccg cctcccgcga gcgcgtcctg
     1381 accgagctcg gcacgacgct gttcgtggag gccggcgcgg gcaccggcaa gaccaccgcc
     1441 ctggtggggc gcatcctgtc gctggtggcc accggcgagg cgcagctgcg cgaggtcgcc
     1501 gcgatcacgt tcaccgaggc ggcggccgcg cagctgcgcg accgcgtccg tgcagcggtc
     1561 gagcacgccg ccggcgacac cggccggccg gccgagcagc gcgagcgcat ggcggtggcc
     1621 gcgacgcagg tcgacgaagc ggccatcctg acgctgcacg ggttcgcgca agcggtcctg
     1681 gccgagcacc ccatcgaggc cggcctgccg cccgagttcg agatcgtcga cgagctgcgc
     1741 gccgccgagc tgctcgacga ggcgtgggcc gagctgcagg aggacctcct cagcgacccg
     1801 ggcctgcgcg cgccgatgga gaccgcgctc acgctggggt tgacgctgta ttcgctggag
     1861 cggctcgcgc gcgccgcggc cgaggcatac gaccgcctgg cggaacaccc cccggcaggc
     1921 gcccccccgg gccgggcgcc ggcggaggac ctcgcggacc gcatcgccgc cacggcgcgg
     1981 tggcgcgcgc gcaacctgcg gccggacgcg ctgctggacc acctcgacga cctggcggag
     2041 ctcgcccggg cgctgcgcgc ggcgcccggc gagctggagg gcctgcgcct gctggccggc
     2101 cggaagctgt cctgctcgcg cgggcagaag ggcgactggg acggcggcgc caccgacatc
     2161 aagcacggcc tggcggacct ggagcggcag cggcgcgacg tgctggcgca cgcgggccgg
     2221 acggcgctcg cgcccatcct cgagcgcgtg cgcgcgtggg ccctggcaca ggcgcggcgc
     2281 cggcggacgc agggccagct cacgttccac gacttgctgg tggcggcgcg cgacctgctg
     2341 cgggaccacg ccgagacgcg cggcctggcg gcgtcgcgct ggcggcggct gctgatcgac
     2401 gaattccagg acaccgaccc gctgcaggtc gacatcgccc tggcgctggc ggagaccgac
     2461 ccgcgcccgg ggcggctgtt cttcgtcggt gaccccaagc agtcgatcta ccgcttccgc
     2521 cgcgccgaca tcgccgtgta cggcgccgcg cgggagcgct tcgccgacgg cctggtgccg
     2581 ctggtggcca acttccggac cgtgccgggc gtggtcgcct gggtgaacgc cgtgttcgcc
     2641 gggctgatgg acggagagcc cggccaggcg ccgttcgcgc cgctgctggc gatgcgccct
     2701 gcgctgcgtg acggcccggc ggtgcacgtg gtgggcgggc cctgcgagaa gctgccggcg
     2761 gcggaggcgc gcgagcgcga ggccacggca gtcgcacggg ccgtgcgcgc ggtgcgcgac
     2821 gagggctggc cggtgcacga cgcgcacgac aaggtcacgc gccccgcgcg ctacgccgac
     2881 atcgccctgc tcctgccggc gcgcaccgcc ctgcccgcgg tcgagcgcgc gctggaggac
     2941 gccggcgtgc cgtaccgggt ggaggcgcgc tcgctggtgt acgagtccca ggaggcgcgc
     3001 gagctgctgg cgctgctgcg cgccgccgac gaccccaccg acgaggtcgc cgtcgtggcg
     3061 gcgctgcgcg gtcccgcgct ggcctgcccg gacgacgcgc tggcgcagtg ggccgccgcc
     3121 ggcggcggct gggactaccg ccaggccgcg cccgccgggg cacccgacat cgtggccgag
     3181 gcgatgcgcc gcctggccgc ctgccacgcg gcgcgcttcg ccgcggaccc cgccgcgctg
     3241 gtcgacctgc tggtgcgcga gctgcggctg ctggagctgg gcgccggccg gccgcgcccg
     3301 cgcgacgcct ggcgccgcct gcgctacgtg atcgacgagg cgcgcgcgct ggccgagcgg
     3361 ggcggctcgc tgcgcgagct ggtgcgccgg atcgagcgcc aggccgccga ccaggtgcgc
     3421 gtcaccgagc aggtgctgcc cgacgaggac gacgacgcgg tgcgcatcct gaccatgcac
     3481 gccgccaagg gactcgagtt cggcgtcacc gtcctgtgcg gcctggccgg cgccggcggc
     3541 ggaggcggcg acccgctgct gtgggacgcc ggcgggcaag ggcaggtgcg cctgggcgac
     3601 cacctggaga cgcagggcta cgcggaggcc aaggaggccg aacgcctggc gcagcgccag
     3661 gagcaccatc gcctcctgta cgtggcggcc acccgcgcgc gcgaccacct gatcctgtcc
     3721 ctggcgcaca cgccgcccaa ggcgtcgccg gccggacgcg aaccgcaggg cgccgagctg
     3781 ctgtgggaac ggagcgcggg ctgcgcgcag ctgtggcggc gctgggagcc gccggagccg
     3841 gtggacgtgc gcgaccccgc ggaaggtgcc gccgcgccgg cgggccccgc cgacgctgcc
     3901 ggcctggccg ccgcggagcg cgcggccgcg caggcgtggc tcgacaagcg gggcgccgca
     3961 ctgtcctctc gcgcgaggct gcccgtgggc gccgcgacgg cgctgaccgc gcaggaccgc
     4021 ccggagcact ccgggcacga cccggcagac gactcgggag agcacccgca ggctgacgag
     4081 cacgcggccc tgccgccgtt ccgccgcggc cgcgccggca ccgcgatcgg ccgcgccgtc
     4141 cacgccgtgc tgcagacgat cccgttcgac gcggcggcgg ccctggtcgc cgccgccgcc
     4201 cgcgcccagg ccgacgccga gggcatcccc ggccgcgccg gcgaggtcgc cgcgctggtg
     4261 gcctcggccc tgcgatccgc gccggtgcgc gccgcgtcgg ccgggcgcag ctggcgcgag
     4321 ctgccggtgg ccgcaccggt gggcggcctg ctcctggagg gctacgtcga cctgctgtac
     4381 gagcaggacg gcgagctggt cctggtcgac tacaagagcg accgcgtgga cgacgcgtcc
     4441 ctggccgcca ccaccgagcg ctacgccacg cagctcggcg cctacgccct ggcgctggaa
     4501 gcggtgctca gccgccgtgt cgcgcgcgcg gccctggtgt tcgtgcgcga gggcgaggcg
     4561 cgcgtcgcct gggtggacga cctggcggcg gccgcggcgc gcgcgcggga gctggtggcg
     4621 gcgcacgccg cgccgcccag cgggtagaac agcggggcac cgcgccggcc gtcggcgcgc
     4681 cgttggcaag gagccgtgtg ccgcccgccc cgcccctgcg cctgcacccc gaggtcgccg
     4741 acgcgctcgc ccacggcacg gccgtcgtgg cgctggagag cacgctcatc gcccacggcc
     4801 tgccgcgtcc gggcaacctc gacgccgcgc gatcgctgga gtccatcgtg cgcgccgccg
     4861 gcgccgtccc ggcaaccgtc gcggtgctcg gcggcgtcgc ccacgtcggc ctggacgacg
     4921 acgccctcac gcgcgtcgcg cacgacgacc tgcgcaagct cgcggtgcgc gacctgcccc
     4981 tggcgctcgg ttcgggcgcc gacggcgcca ccaccgtgtc ggccaccgcc acgctcgccg
     5041 cgctcgcggg catccgggtg ttcgccacgg gcggtctcgg cggcgtgcac cgcgaagcgg
     5101 cgcagacctg ggacgagtcg gccgacctgc cggcgctcgc gtccaccccg gtggcggtgg
     5161 tgtgctccgg tgtgaagtcc atcctggaca tccccgccac gctggagcgc ctggagacgc
     5221 tcggcgtgcc cgtcgtcggc taccgcaccg cgcgcttccc cggcttctac ctgagcgaca
     5281 gcggccagga cctcgactgg cgcgccgact cccccgagga ggtcgcggcc atgctgcgcg
     5341 ccgaggacgc gctgggcggc ggccgcggcc tggtcgtggc caaccccgtg ccggccggcg
     5401 agcagctcga cccgcgcctg cacgacgacg tgctgcgagc gggcctggac gcgctggcgg
     5461 cgcagggcgt gcgcggcaag gacgtgacgc cgttcctgct gtcgtggatg cacgagcaca
     5521 ccggcgggga gtcgctgcgc gtcaacgtcc gcctggtcga gcacaacgcc cgcctcgccg
     5581 cccggatcgc cgtcgcgtac gcctcatgaa ggtgctcgtg gtcggcgatc tggtcgtcga
     5641 tgtcgtggcg gcgccgaagg gttcggtggc cgtggacagc gacaccgctg cgcgcgtcgc
     5701 gctgcggggt ggcgggtccg cggcgaacgc ggcggcgtgg ctggcgctgg ccggcgccca
     5761 ggtggcgctg gtcgcgcgca tcggcgacga catggcgggc cggctgctgc gcgaggaact
     5821 gtcggccggg cgcgtcgagc cgctgctggc ggtcgacgcc tccgcggcga ccgggaccat
     5881 cgtggtgctc gtcgagcccg acgggcgccg cacgatgctc accgaccggg gtgcctccgc
     5941 gctgcttgcg ccccgcgacc tgccggcgga gcgcttcacc gcgcccgccc acctgcacct
     6001 gtccggttac acgctgctcg ggcaggcgtc gcgcgccgcc ggcctgcacg ccctgcgcct
     6061 ggccgcggcc gcgggcatga ccagctccgt cacgccgggc accgccgagc cgctgcgcca
     6121 ggcgggcgcc gagtcgttcc tgtcctggac cgccggggcg acgtgctgcc tggccaaccg
     6181 cgaggaggcc ggcgtgctga ccggcgccgg acccggcgcc ccgcacgccg aactcgcgct
     6241 ggccctggcc gagcgctatg ccgaagccgt cgtcaccgcc ggagcggacg gcgccgcgtg
     6301 ggcgtccggc accgaggtcg tcgccttgcc cgcggtcgac gccgccgccg tggacaccac
     6361 cggcgcgggc gacgccttcg cggccgggct gctcgcggcc tggctcggtg gggcgtcgcg
     6421 ggaagacgcg ctgcgcgcgg ggctgcggct ggccgcgcag gcggtggcac tcgccggcgg
     6481 gcgtccgccc gcggcggtct cggacatggg tctagggtga tggcggcccg ggccacccgg
     6541 cgggcaccag gaggatgacg tggagcgcgc gaccgacgac acccgccccc ggaccgcgtt
     6601 cgtcgccgga ggcggcggcg cgctcggcca ggcggtcgtg gcccgcctgc tcgacgccgg
     6661 ggagcgcgtg tgcgtggcgt gggtggtcga ggacgaagcc gccgcgctgc gtgcgcgctt
     6721 cggcgaccag gccgacagcg ggcggctgcg gctgagccgc tgcgacgtcg ccgacggcga
     6781 ccaggtggcc gccctgctgg acgtgctgga ggccgactgg ggaccgctgt ggctggcgtg
     6841 ctcggtggtg ggcggctggg ccggcggcca gaccgtcgag gagctggacg acctcagcgt
     6901 gctggaccgg atgctgctgc tgaacctgcg caccgccacc gcggtcgcgc gcgagggcct
     6961 gcgccacatg ggcccggcgg gcgggcgcgt ggtgctcacc gcgtcgcgca ccgcgctggt
     7021 cccggcgccc tcgcaggctg cctacagcgc cgccaaagcg gctgtgctca gcctggtcgc
     7081 cacgctggcc gaggagctgc gcgggtccgg gcgcacggcc aacgccatcg tcccgaaggt
     7141 catcgacaca cccggcaacc gccaggccat gccggacgcc gaccacagcc gctgggtgcc
     7201 gccggaggcg atcgccgagg tggtcgcctg gctcggctcg gccgcgtcgt ggcccgtcag
     7261 cggagcagcc gtgccggtct acggggacgc ctgaggtgcg ccgcgtcacc gtgcggccgg
     7321 ctctgctcct ggccgccttg ctcctggccg gcgccgcctg cgcggacgag ccgcgccgct
     7381 cggccaccga cgggccgggt gaggcgccgg gcgcgtgcga gccggtggag cggccgcccg
     7441 agcagtccgg cggccacctg ctgggcgagg cgacgccccc ggtgccgtac tcgtccacgc
     7501 cgccgacgtc cggctggcac gcctcgggca acccggcgcg cggcgtccac ggtgccggcg
     7561 acccgctgtc ggagcctgag caggtcacga tcctggagct cggcggggtc gtgctctcct
     7621 ggaacgggct ggacggcgac gcgcgcgagc ggctggagtc ctacgcacgc gagcacgaag
     7681 acacggtggc ctcgacgccg tacggggcac tggcccccgg cgaggtggcc atgaccgcct
     7741 ggggcgtgct ccagcgctgc gacggcgtgg acctggaggc ggtggacgcg ttcgtggccg
     7801 agcacgccgg cggcggcccc gaccactgag ccggtcccac tgaggttgag aatcattctc
     7861 agttgcggca gcatggagcc cgttcgtccc gctgccgacc cggagggccc cgtgcgccgc
     7921 atccccaccc tgctctgcct cgccctgctg gccgcggcct gcggcgacgc gccgtcgggc
     7981 caggaccggc cgctcgtggc agcgacgccg tggcccttgc agtggctggc cgagcaggtc
     8041 gcccccgagg cggaggtgcg cggcctgggg caggcgggcc aggacccgca cgaactggac
     8101 ctgcgcccgg ccgagcggga gctgctggag cgcgccgacg tggtgctgtt cgtgggaccg
     8161 gtcggcttcc agccgcaggt cgaggaggcc gccgccggcc gcggcgaggc cgccgtggcc
     8221 atcacgcagg cggcggcgga cctgctgctg gacgcggagg cgcacgagca cgccgaagac
     8281 gagcacgccg aggacgagca cgccgaggac gaggcggtgg acccgcacgt ctggttcgac
     8341 ccgcgcaccc tggccgaggc cgcggacgcg ctggccgagg ccttggccgc cgtggacccg
     8401 gaccgcgccg acggctaccg cgagcgcgcc gcggcggtgc gcggccggct gctcgcgctg
     8461 cacgagg
//
LOCUS       DATMKC010000270         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_135051, whole genome shotgun sequence.
ACCESSION   DATMKC010000270 DATMKC010000000
VERSION     DATMKC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135051"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VNU01_07970"
     CDS             <1..920
                     /locus_tag="VNU01_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HWH32594.1"
                     /translation="LGVVHVRQAEALGIARQTLARRAAEGGWKRLHPGVYLLPAFVAD
                     YRTRVSAALLALGPGAAAGGPTAAALWGLVEGEREPLQVLLPHDRPVRALDGVRVVRS
                     RSVTAGDIVRLGPLAVTTIDRTLCDLAGVLVEEELREAVALAGQRSMQWPRRAAARAA
                     ELPRLAGRAALLTAIRDVLGEGRTDSTLERRLRRILKAHGLHPAPGVYPLIVNGRLLA
                     MLDIAFPELRLAVEADGFAFHATPAQLRADHARQNRIAAAGWTILRVGKEEVAAGAEH
                     LVSEIAARAAAADEAVLRRAAARGATVRG"
     gene            complement(952..1926)
                     /locus_tag="VNU01_07975"
     CDS             complement(952..1926)
                     /locus_tag="VNU01_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32595.1"
                     /translation="MRARGLLAIAAAVLAAAIALPAHAQDAPPMGLSLTPGVVEREVA
                     GRDVDLPFTIVNNDTNPVDVALSVQRLSQTVEGAFDYTGPAGLVVEPALVRLLPGERA
                     TVRVTGALPDGAAALYAGLLAEPRIIRPAGGNVEVRTRVAGLLLLTTPGAHRRAVEIT
                     DVSLTPTAEEGTYRVGAVLRNTGDVHVRPQGVVEISEPGGPVLGTATLAGAVLLPGAE
                     RRLDGGLWTAPAQLPARVDLDVAMADPVARGGRSVALTGPDAVAPPVVPGEDLGELGG
                     DDPAPGRSWLVWLALGLLFAVSALILWERGRVSSVGLPDTAVSCQGGG"
BASE COUNT          250 a          732 c          827 g          247 t
ORIGIN      
        1 aactgggcgt ggtgcacgtc cgtcaggcgg aggcgctcgg catcgcgcgg cagacgctgg
       61 cgcggcgggc cgccgagggc ggctggaagc gtctccatcc aggcgtctac ctgctgccgg
      121 cgttcgtggc cgactatcgc acgcgcgtct cggccgcact ccttgccctt gggcctggtg
      181 ccgcggcggg cgggcccacc gcggcggcgc tgtgggggct ggtcgagggc gaacgggaac
      241 cgctccaggt gctcctgccg cacgacaggc ccgtccgggc gctcgacggt gtgcgggtcg
      301 tgcggtcccg gagcgtgact gccggggaca tcgtgcgcct cgggcccctg gcggtcacga
      361 cgattgatcg gacgttgtgc gatctggccg gggtgctcgt ggaggaggag ttgcgcgagg
      421 cggtggcgct ggccgggcag cgctcgatgc agtggccgcg ccgcgcggcc gcgagagcgg
      481 ccgagctgcc ccggctggcc gggcgggcgg cgctgctgac ggccatccgt gacgtgctgg
      541 gggagggacg gaccgactcg accctggagc ggcgtctccg gcggatcctc aaggcacacg
      601 gcctccaccc cgcaccgggc gtctacccgc tcatcgtgaa cgggcggctg ctcgcgatgc
      661 tggacatcgc ctttccggaa ctgcgcttgg ccgtcgaggc cgacggcttc gcgttccatg
      721 ccacccccgc gcagctgcgc gccgaccacg cccgccagaa ccgcatcgcc gccgccggct
      781 ggaccatttt gcgcgtcggc aaggaggaag ttgcggcggg agcagagcac ttggtgagcg
      841 agatcgcggc gcgggcggcg gccgcggacg aggccgtact ccgaagggcg gcggcacgcg
      901 gcgccacggt gcgcggctga gaaggaagcg ggtacggcga ggggcgcggt atcagccgcc
      961 gccctggcag gagacggccg tgtccggaag gccgacggag gagacgcggc cccgctccca
     1021 caagatcagc gcgctcaccg cgaagagcag cccgagcgcc agccacacca gccaggaccg
     1081 gccgggggcg gggtcgtcgc cgccgagctc gccgaggtcc tcgcccggca ccacgggcgg
     1141 cgccaccgcg tccggcccgg tcagcgccac ggaccgcccg ccgcgcgcca ccgggtcggc
     1201 catcgccacg tccaggtcca cgcgcgccgg caactgtgcc ggcgcggtcc acagtccgcc
     1261 gtccaggcgc cgctcggcgc cgggcagcag caccgcgccg gccagcgtgg cggtgcccag
     1321 gaccggcccg cccggctcgg agatctcgac cacgccttgc ggccggacgt gcacgtcgcc
     1381 ggtgttgcgc aggacggcgc ccacccggta cgtgccctcc tcggcggtcg gggtgagcga
     1441 cacgtcggtg atctcgacgg cgcggcggtg cgcgccgggc gtggtgagca gcagcagccc
     1501 ggccacgcgc gtgcggacct cgacgttgcc gccggcgggc ctgatgatgc gcggctcggc
     1561 gaggaggccg gcgtagaggg cggcggcgcc gtcgggcagc gcgccggtga cgcggacggt
     1621 ggcgcgctcg ccgggcagca ggcggaccag cgcgggctcg accaccagtc cggccgggcc
     1681 ggtgtagtcg aacgcgccct cgacggtctg cgacaggcgc tggacgctca gggccacgtc
     1741 gacgggattg gtgtcgttgt tgacgatggt gaagggcagg tcgacatcgc ggccggccac
     1801 ctcccgctcc acgacgccgg gggtgagcga cagccccatg ggcggggcgt cctgcgcgtg
     1861 cgcgggcagc gcgatcgccg cggccaggac ggccgcggcg atcgcgagca ggccgcgtgc
     1921 gcgcatctgc ttacagcgcc gctgcggcgg tgtaggtgag caccgtgcgg tacgtgcccg
     1981 gtgtgtccga ggcgagcacc gtctgggcca gctggagcgt ggcgctgtcg gtggtcagcc
     2041 ccgtgccggt catgag
//
LOCUS       DATMKC010000271         7369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_215789, whole genome shotgun sequence.
ACCESSION   DATMKC010000271 DATMKC010000000
VERSION     DATMKC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215789"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VNU01_07980"
     CDS             <1..689
                     /locus_tag="VNU01_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWH32596.1"
                     /translation="SVVGPHPFPLLVREFVKVIGQEVRAQLLETEGRLPDAVVACVGG
                     GSNAMGAFHDFVREPSVRLVGVEAGGEGVASGRHSSTLGGGSEGILHGARSFVLQDEF
                     GQVLPTHSVSAGLDYPGVGPEHAWLKDTGRAEYVPATDDEALEGFGLLCRLEGIIPAL
                     EPAHAVLPALRVARELGPDGIVVLNVSGRGDKDVDEVASILAVRTPTDEAGAEPKAVD
                     PGAVDPGVGA"
     gene            686..1483
                     /gene="trpA"
                     /locus_tag="VNU01_07985"
     CDS             686..1483
                     /gene="trpA"
                     /locus_tag="VNU01_07985"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWH32597.1"
                     /translation="MNAVEAAIRAANADGRAALIAYLPAGYPSLTDSRQCLEAAVEAG
                     ADLLEVGFPYSDPLMDGPTIQAASQVALDQGLTPADDFALCRELTANVGVPCLVMTYY
                     NLAWHYRGVERLEDFAAEARDSGLAGAILPDLPAEEGAPWCAVAEAHGLATVFLAAPT
                     SADERLAAVARSATGFVYATSTLGVTGERQSLSDMAAPLVARLRAVTDKPVCVGIGVT
                     TGEHAAEVAGFADGVIVGSAIVRAAGEGGPEAVRALVKELADGVRRR"
     gene            complement(1548..1739)
                     /locus_tag="VNU01_07990"
     CDS             complement(1548..1739)
                     /locus_tag="VNU01_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32598.1"
                     /translation="MSTHLNGDGPEHDPVESNRATNPRRTPGRAELGVDVTRRVTIAA
                     MVVVAGATAILIAILSSTS"
     gene            1738..2199
                     /locus_tag="VNU01_07995"
     CDS             1738..2199
                     /locus_tag="VNU01_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32599.1"
                     /translation="MAAHGSAVVLEVVAERPDDLPGTVALLFDHAERRGLRLSWRVDD
                     LTDDALLFRLAFGRHGVVPAGREGTGRLFAKPLAADPLAWLQGAQLAVGAAVRGEGPV
                     RLVLDLAELDRPDAFSVVVEALDLIEGLVAAGRLRMLTLADLAEEHPDERS"
     gene            2262..2855
                     /locus_tag="VNU01_08000"
     CDS             2262..2855
                     /locus_tag="VNU01_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWH32600.1"
                     /translation="MGRPRSWTDEQLIEAVAASETLLEVLERLGLKRGGGTLWSDADL
                     AQAVATSFSMAQTIAAMGLRVGGSVYPMLREHITRLGLDTSHWTGNGRPRGSTFPGRN
                     GRPLADVLVADSDYRNTDRLKKRLLRAGLKEPRCEICGLAEWRDAPIPLQLDHVNGVR
                     SDNRLENLRILCPNCHAQTETWCGRNIGNGRGTVSDR"
     gene            2885..2969
                     /locus_tag="VNU01_08005"
     tRNA            2885..2969
                     /locus_tag="VNU01_08005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Leu,seq:caa)
     gene            3076..3441
                     /locus_tag="VNU01_08010"
     CDS             3076..3441
                     /locus_tag="VNU01_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32601.1"
                     /translation="MRRYGNLQRRLTEARDELARLRESLRILDEQVAFQQGVADEAET
                     RAVVSQTPLADREARDAQGDLRRLLRQREEVAATIAELAAEQDQLLDRLLEQQATARP
                     AEGWGEREWNEPATRRQED"
     gene            3446..4114
                     /locus_tag="VNU01_08015"
     CDS             3446..4114
                     /locus_tag="VNU01_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH32602.1"
                     /translation="MSPAAEISANATQGHQADVDESLLDAEETRPVRVLIAEDEALIR
                     LDLKEMLTEEGFEVVAEVADGAAAVERARELRPDLAILDIKMPVMDGLEAAERITDER
                     LAPVLILTAFSQRDLIERARRAGAMAYLVKPFQKHDLLPAIEIAAGRFADLQGLASQV
                     GTLQDRLEARKLIERAKGLLQQHEGVSEQEAFRSLQRQAMTDRLSMRAVAESVIARYG
                     AETA"
     gene            4226..5077
                     /locus_tag="VNU01_08020"
     CDS             4226..5077
                     /locus_tag="VNU01_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWH32603.1"
                     /translation="MKLGMRWLLALAAVMALLLTACGDAAEEEPQGDDTDTETENEGG
                     DDLGLIQEGRLLVGSDINFAPFEFIEDGEEKGFDIDLMNEIAERLGLEVEYVNTGFDN
                     IFTQLAGGEFDVIVSAITITDERKEIIAFSEPYFEANQALVTTQDSGISGVADLAGKD
                     VGTQAGTTGLDYARENFTDASIVEFPDAPAGFTALAAGQLDAMFIDVPVAVEQVEGND
                     DFVLVEEVSTNELYGIGVQQDDAALLEAIDAALAEIIADGTYAEIFETWFPGAQVPDR
                     FATDGAS"
     gene            5105..5845
                     /locus_tag="VNU01_08025"
     CDS             5105..5845
                     /locus_tag="VNU01_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWH32604.1"
                     /translation="MDKFARYFLDWERMAQVFPDLLREGLRNTLVLSLGAMAIGLVAG
                     MVLALLAISRRWWVRAPARVYIDLFRGLPAILVIVLIGTGLPLSGYRVFGREKFLYGI
                     LALGLIATAYISEIFRAGIQSVDKGQMESARSVGMTYLQAMRLIVVPQGIRRVLPPLT
                     NEFIALVKDSSLIFIIGISVGERELFRVGQNYTQQHGNMSALVAAGLFYVAITVPLAH
                     LVNFMDKRLREGKRTTADALPAGAGEHA"
     gene            5842..6582
                     /locus_tag="VNU01_08030"
     CDS             5842..6582
                     /locus_tag="VNU01_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWH32605.1"
                     /translation="MSATAPRVALRGLRKRFGNAEVLRGIDLEIAAGEVVCVIGPSGS
                     GKSTMLRCVNRLEEPSGGAVEIDGQDITGPGVDINAIRSRVGMVFQHFNLWPHKTALA
                     NVALAPRKVKGLSRAEAEKIARARLEEVGLADKADARPSHLSGGQQQRVAIARALAMD
                     PDVMLFDEVTSALDPELVKEVLDVMRGLAREGMTMIVVTHEMGFAREAAHRVVFLDHG
                     RVVEEGPPQRLFDDPHTERLRTFLGQVL"
     gene            6669..>7369
                     /locus_tag="VNU01_08035"
     CDS             6669..>7369
                     /locus_tag="VNU01_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32606.1"
                     /translation="MPTLPRLLAVFALALLCLAAPAYAQGQEAPGGEAVKGTLTHEDE
                     PVEGAVIVVETEDGEPVGEVESDDDGSFQLEMPGPGRYRATLDEASLPEGVGLRDPER
                     ATLEFQLRTGQQRTLIYALGERQSTASVLLPRIAQQAAQGLRFGLVIAITAIGLSLIF
                     GTTGLVNFAHSEMVTFGGVAAWYLNARGPRIPLIAAGALAVVLGGLLGGGLERGLWRP
                     LRERKTGLIQMLVIS"
BASE COUNT         1039 a         2506 c         2772 g         1052 t
ORIGIN      
        1 gttcggtcgt gggccctcac ccgttcccgc tgctggtccg cgagttcgtc aaggtcatcg
       61 gccaggaagt ccgcgcccag ctgctggaga ccgagggtcg cctgcccgac gctgtcgttg
      121 cgtgcgtcgg cgggggcagc aacgcgatgg gggcgttcca cgacttcgtg cgcgagccgt
      181 cggtgcgcct ggtgggcgtg gaggccggcg gcgagggcgt ggcctcgggc cggcactcct
      241 cgacactggg gggcggcagc gagggcatcc tgcacggggc gcgcagcttc gtgctgcagg
      301 acgagttcgg ccaggtgctg ccgacccact cggtgtccgc gggcctggac taccccggcg
      361 tgggccccga gcacgcctgg ctcaaggaca ccggcagggc cgagtacgtg cccgcgaccg
      421 acgacgaggc gctggagggc ttcggcctgc tgtgccgcct ggagggcatc atcccggcgc
      481 tggagccggc gcacgcggtg ctgccggcgc tgcgcgtggc gcgcgagctg gggcccgacg
      541 gcatcgtggt gctgaacgtg tccgggcgcg gcgacaagga cgtggacgag gtcgcctcga
      601 tcctggcggt gcgcacgccg accgacgagg ccggcgccga gccgaaagcc gtggacccgg
      661 gggccgtgga cccgggggtg ggcgcgtgaa cgcggtcgag gcggcgatcc gcgccgcgaa
      721 cgccgacggc agggcggcgc tgatcgccta cctcccggcc ggctacccgt ccctcacgga
      781 ctcccgccag tgcctggagg cggccgtgga ggcgggcgcc gacctgctgg aggtgggctt
      841 tccgtactcc gacccgctca tggacgggcc gaccatccag gcggcgtcgc aggtggcgct
      901 ggaccagggg ctcacgcccg ccgacgactt cgcgctgtgc cgcgagctga ccgcgaacgt
      961 cggcgtgccg tgcctggtga tgacgtacta caacctcgcc tggcactacc gcggggtgga
     1021 gcggctggag gacttcgccg ccgaggcgcg cgacagcggg ctggccggcg cgatcctgcc
     1081 ggacctgccg gccgaggagg gcgcgccgtg gtgcgccgtc gcggaggcgc acggcctggc
     1141 gacggtgttc ctggccgcgc cgacctcggc cgacgagcgc ctggccgccg tcgcgcggtc
     1201 ggccacgggc ttcgtctacg ccacctcgac gctgggcgtg accggcgagc gccagtcgct
     1261 gtcggacatg gccgcgccgc tggtggcgcg gctgcgcgcg gtgaccgaca agccggtgtg
     1321 cgtcggcatc ggcgtgacga cgggggagca cgccgcggag gtcgcgggct tcgccgacgg
     1381 ggtgatcgtc ggttcggcga tcgtgcgcgc ggccggggag ggcggccccg aggccgtgcg
     1441 cgccctggtc aaggagctgg cggacggggt ccggcgccgc tgacccccgg cccccagatg
     1501 ggtgcaggtt gggcgctccc tggcccggga tgcacccgtt ggggccgtca ggatgtggac
     1561 gagaggatgg cgatcaggat ggcggtcgca ccggccacga cgaccatcgc cgcgatcgtg
     1621 acgcgccggg tcacgtccac gccgagctct gcccgccccg gcgtccgccg cgggttcgtc
     1681 gcacggttgg actcgaccgg gtcgtgctcc ggcccgtcgc cgttgaggtg agtgctcatg
     1741 gccgcacacg gtagcgccgt cgtcctggag gtcgtggcgg agcgacccga cgaccttccg
     1801 ggcacggtgg cgctgctgtt cgaccatgcc gagcgacgcg gcctgcgcct gagctggcgc
     1861 gtcgatgacc tgaccgacga cgcgctgctg ttccgcctgg cgttcggccg gcacggcgtc
     1921 gtgccggccg ggcgggaggg cacagggcgg ttgttcgcga agccgctggc cgcggacccc
     1981 ttggcgtggc tgcagggcgc gcagctcgcc gtgggcgccg ccgttcgagg ggagggccct
     2041 gtgcgcctgg tgctggacct ggctgagctg gatcgccctg acgcgttcag cgtggtggtc
     2101 gaggcgctcg acctgatcga ggggctggtc gccgccggcc gcctgcgcat gctgacgctg
     2161 gccgatctgg cggaggagca tcccgacgag cgctcctgaa ctgtgcacaa ccacggtcca
     2221 gcgggccgtt tctgtcacag gcggacggga cgcttggcgg catgggacga ccgcgaagct
     2281 ggaccgacga gcagctcatc gaggcggtgg cggcatcgga gacgttgctt gaggtgctcg
     2341 agcgccttgg gctgaagcgc ggcgggggca cgctctggtc cgacgccgac ctggcacagg
     2401 cggtggccac cagcttctcc atggcgcaaa ccatcgccgc gatgggcttg agggtcggcg
     2461 gctcggtgta tccgatgctc cgtgagcaca tcactcgcct cggcctcgac acgtcccact
     2521 ggacgggcaa cggcaggccg cgaggctcta cgttccccgg gcggaacggg cgtcctctcg
     2581 ccgacgttct cgtcgctgac agcgattacc gcaacaccga ccggctcaag aagcgactgc
     2641 tgcgggccgg cctgaaggag ccccgctgcg agatctgcgg gttagccgag tggcgggacg
     2701 ctcccatccc gttgcaactg gaccatgtga acggggtgag gagcgacaat cggctggaga
     2761 acctccgcat cctgtgcccc aactgccatg cgcagaccga gacctggtgt ggtcgcaaca
     2821 tcggtaacgg gcgaggtact gtgtcggatc ggtagacgcg gagctgaggc ccgcgaatgc
     2881 ctttgccccg gtggcggaat caggcagacg cgcgtcactc aaactgacgt attcggaaga
     2941 atgtgtgggt tcgaatccca cccggggcac tcggattcgt gtgcgcgcgg gctcgcgtac
     3001 actccctgtc cccgcctgac cctgccggtg actcttggtc cccgcacacg ggtccgggct
     3061 cacgaagagg tgcgagtgcg gcggtacggc aacctgcagc ggcggctgac ggaggcgcgc
     3121 gacgagctcg cgcggctgcg cgagtcgctg cgcatcctgg acgagcaggt ggcgttccag
     3181 cagggtgtcg cggacgaggc cgagacccgg gcggtggtgt cgcagacgcc gctggccgac
     3241 cgcgaggcgc gggacgcgca gggggacctg cgccggctgc tgcggcagcg cgaggaggtg
     3301 gcggcgacga tcgcggaact cgcggcggag caggaccagc tgctcgaccg cctgctggag
     3361 cagcaggcca cggcgcgacc cgcggagggc tggggcgaac gagagtggaa cgagccggcg
     3421 acccggcgac aggaggactg acgagatgag cccggcagcc gagatctcgg cgaacgcgac
     3481 gcaggggcac caggccgacg tggacgagtc cctgctcgac gccgaggaga cccgcccggt
     3541 ccgcgtgctg atcgccgagg acgaggcgct catccgcctg gacctcaagg agatgctcac
     3601 cgaggagggc ttcgaggtgg tcgccgaggt ggccgacggc gccgccgcgg tggagcgcgc
     3661 ccgcgagctg cggcccgacc tggccattct ggacatcaag atgccggtca tggacggcct
     3721 ggaggccgcc gagcgcatca ccgacgagcg cctggccccg gtcctgatcc tgaccgcttt
     3781 cagccagcgc gacctcatcg agcgggcccg gcgggccggc gccatggcgt acctggtcaa
     3841 gccgttccag aagcacgacc tgctgcccgc catcgagatc gcggctggcc gcttcgcgga
     3901 cctccagggg cttgccagcc aggtcggcac cctccaggac cgtctggagg cgcgcaagct
     3961 gatcgagcgc gcgaagggcc tgctccagca gcacgagggc gtcagcgagc aggaggcgtt
     4021 ccgctcgctg cagcgccagg ccatgacgga ccgcctgtcc atgcgcgccg tcgccgagtc
     4081 ggtcatcgcc cggtacggtg ccgaaacggc ctgacggcga tcttgacgga aagggagtcg
     4141 gccatccccc cggtggggag gatgggttag gctccgtcgc cgccgagggt gggttctgta
     4201 gccccagact ttcgaggaga caatcgtgaa gcttggcatg cgctggctct tggccctcgc
     4261 cgccgtcatg gctctgctgc tgaccgcgtg cggggacgcg gccgaggaag agcctcaggg
     4321 cgacgacacc gacaccgaga ccgagaacga gggcggggac gacctcggcc tgatccagga
     4381 gggcaggctt ctggtcggct cggacatcaa cttcgcgccg ttcgagttca tcgaggacgg
     4441 tgaggagaag ggcttcgaca tcgatctcat gaacgagatc gcggaacggc tgggcctgga
     4501 ggtcgagtac gtcaacaccg gcttcgacaa catcttcacg cagctcgccg gcggcgagtt
     4561 cgacgtgatc gtctccgcga tcacgatcac cgacgagcgc aaggagatca tcgccttctc
     4621 ggagccgtac ttcgaggcga accaggcgct ggtgaccacg caggactccg gcatctccgg
     4681 ggtggccgac ctcgccggca aggacgtcgg cacccaggcg ggcaccaccg gcctggacta
     4741 cgcgcgcgag aacttcaccg acgccagcat cgtggagttc cccgacgcgc ccgccggctt
     4801 caccgcgctg gcggccggcc agctggacgc gatgttcatc gacgtgccgg tggccgtgga
     4861 gcaggtcgag ggcaacgacg acttcgtcct ggtggaggag gtctccacca acgagctgta
     4921 cggcatcggc gtgcagcagg acgacgcggc gctgctggag gcgatcgacg ccgcgctggc
     4981 ggagatcatc gccgacggca cctacgcgga gatcttcgag acctggttcc cgggcgcgca
     5041 ggtgccggac cgcttcgcga ctgacggcgc gtcgtaggca ccgcggcaac ggtcgctcgc
     5101 gcccatggac aagttcgcca ggtacttcct ggattgggag cgcatggcgc aggtcttccc
     5161 ggaccttctc cgggaaggcc tgcgcaacac tctcgtgctg tccctgggcg cgatggcgat
     5221 cggcctggtc gccggcatgg tgctcgccct gctcgcgatc tcgcgccggt ggtgggtgcg
     5281 cgcgccggcc cgcgtctaca tcgacctgtt ccgcgggctg ccggccattc tcgtcatcgt
     5341 gctgatcggc acgggcctgc cgctgtcggg ctaccgggtg ttcggccgcg agaagttcct
     5401 ctacgggatc ctggcgctgg gcctcatcgc caccgcctac atctccgaga tcttccgcgc
     5461 cggcatccag agcgtggaca agggccagat ggagtcggcg cgcagcgtcg ggatgaccta
     5521 tctgcaggcg atgcggctga tcgtggtccc gcagggcata cgccgcgtgc tgccgccgct
     5581 gaccaacgag ttcatcgccc tggtgaagga ctccagcctg atcttcatca tcgggatctc
     5641 ggtgggtgag cgggagctgt tccgcgtcgg ccagaactac acgcagcagc acggcaacat
     5701 gtcggcgctg gtggcggccg ggctgttcta cgtcgccatc accgtgccgc tggcgcacct
     5761 ggtgaacttc atggacaagc gcctgcgcga gggcaagcgc acgaccgccg acgcgctgcc
     5821 cgccggagcg ggggagcacg cgtgagcgcg accgctcccc gcgtcgcctt gcgcggactg
     5881 cgcaagcgct tcggcaacgc cgaggtcctg cgcggcatcg acctggagat cgccgccggc
     5941 gaggtggtct gcgtcatcgg cccgtccggc tccggcaagt ccaccatgct gcgctgcgtc
     6001 aaccgcctgg aagagccctc gggcggcgcg gtggagatcg acgggcagga catcaccggc
     6061 cccggcgtgg acatcaacgc gatccgcagc cgcgtcggca tggtgttcca gcacttcaac
     6121 ctgtggccgc acaagaccgc gctggccaac gtcgcgctgg cgccgcgcaa ggtgaagggg
     6181 ctgtcgaggg cggaggcgga gaagatcgcg cgcgcccgcc tggaggaggt cggcctggcc
     6241 gacaaggcgg acgcgcgtcc cagccatctg tccggcggcc agcagcagcg cgtggcgatc
     6301 gcccgcgctc tggcgatgga cccggacgtc atgctgttcg acgaggtcac ctccgcgctg
     6361 gacccggagc tggtcaagga ggtgctcgac gtcatgcgcg ggctcgcccg cgaggggatg
     6421 accatgatcg tggtgacgca cgagatgggc ttcgcgcgcg aggccgcgca ccgcgtggtg
     6481 ttcctggacc atgggcgcgt ggtggaggag ggcccgccgc agcgcctgtt cgacgacccg
     6541 cacaccgagc ggctccgcac ttttctcggg caggtcctat gacggcccgc tcgcgctgcc
     6601 tacactctgc ccggcgcggc tccgccgccc gccgcccgcc cggaaaccac ggaccagaag
     6661 gacgccgcgt gcccacgctg ccacggctgc tcgctgtctt cgcgctcgcc ctgctgtgcc
     6721 tggccgctcc ggcctacgcc cagggccagg aggcccccgg gggagaggcc gtcaagggca
     6781 cgctgaccca cgaggacgag ccggtggaag gcgccgtgat cgtggtggag acggaggacg
     6841 gcgagccggt cggcgaggtc gagtccgacg acgacggctc cttccagctc gagatgccgg
     6901 gccctggccg ctaccgcgcc acgctcgacg aggcgtcgct gccggagggc gtcgggctgc
     6961 gcgacccgga gcgcgcgacg ctggagttcc agctgcgcac gggccagcag cgcacgctga
     7021 tctacgcgct gggcgagcgg cagtccacgg cgtccgtcct gctgccgcgc atcgcgcagc
     7081 aggccgcgca ggggctgcgc ttcgggctcg tgatcgcgat caccgccatc ggcctgtcac
     7141 tgatcttcgg cacgaccggg ctggtgaact tcgcgcacag cgagatggtg acattcggcg
     7201 gcgtggcggc gtggtacctg aacgccaggg gtccgcgcat cccgctcatc gcggccggcg
     7261 cgctggccgt cgtcctgggc gggctgctgg gcggcgggct cgaacggggc ctgtggcggc
     7321 cgctgcgcga acgcaagacc gggctcatcc agatgctggt gatctcgat
//
LOCUS       DATMKC010000272         3308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_132514, whole genome shotgun sequence.
ACCESSION   DATMKC010000272 DATMKC010000000
VERSION     DATMKC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132514"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VNU01_08040"
     CDS             <1..1526
                     /locus_tag="VNU01_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32607.1"
                     /translation="ADAAAAAADDAVAAAQRDLATATATVNSIQSTIAGLDADIAALE
                     QELATTLDPVRLVQLPGEIAAKVNSRDAAVASLASAEAARASAASVLSATETSAVAKR
                     GAAATARAALTAAESAMASAVTAADSAATALSKAISQADAAVAAAAAADLAVATAAQQ
                     ADALQARLRTLLQAVSDLLEGMPPLPVLEQELDEALRAAPIVSIGGLALETTVTASPA
                     GSSNLVHCKVERVTVLGLSPEVEECTDLIAMEKEAVRQVNELLQSLGAVGVQGVQLLG
                     PGGTWSQPGPVGPDGKARSEGQMAALTLSIPEVSLDGVAANLLAKVGPILEAIRSRPA
                     SAIIVPTIRPADGKDFVQLAASGPRMEAGSRTLGDQIQVLSDELDNVRPPDELQNLST
                     GSVHAVVAQAKTVAEFDTTRMDDQDTSDPTSVCGGDPVCLAQCAAGNCPGSAAGDDPA
                     AAGGGRGSGSDRMGLPWSGDATGAKLPVTGAAGGLTAVALLALGGAGAGWLRRRPRP"
     gene            complement(1537..1719)
                     /locus_tag="VNU01_08045"
     CDS             complement(1537..1719)
                     /locus_tag="VNU01_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32608.1"
                     /translation="MPTELRQHDLEMLRLDMTRQIREEISKQDNRIFNWAYGTVLGFM
                     LTVYAFVLTWLAMADR"
     gene            complement(1835..2029)
                     /locus_tag="VNU01_08050"
     CDS             complement(1835..2029)
                     /locus_tag="VNU01_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32609.1"
                     /translation="MTVAQEEAASKDDIKRLDNRIDRLSDRVERGFASVHEKLDKLNV
                     THLRSTLALAALIVAVNRWG"
     gene            complement(2083..2310)
                     /locus_tag="VNU01_08055"
     CDS             complement(2083..2310)
                     /locus_tag="VNU01_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32610.1"
                     /translation="MTVAQEEAATKDDIRRLDNRIDRLSDRVERGFAAVHRRIDELQA
                     ELQGKIERLSTNQLRSTLALAALILAVNRWG"
     gene            complement(2349..2552)
                     /locus_tag="VNU01_08060"
     CDS             complement(2349..2552)
                     /locus_tag="VNU01_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="HWH32611.1"
                     /translation="MTVAQEEAASKDDIKRLDGRIDRLSDRVDRLSDRVDRLSERIER
                     LNINHLRSTLALAALIVAVNRWG"
     gene            3129..>3308
                     /locus_tag="VNU01_08065"
     CDS             3129..>3308
                     /locus_tag="VNU01_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32612.1"
                     /translation="MARARRTVLAGVLTLGLVLSLAPGAAAEHYEGTFEVQGATPTEA
                     SVSFSRLTFPGGSAEA"
BASE COUNT          439 a         1188 c         1193 g          488 t
ORIGIN      
        1 ccgctgacgc cgcggcagca gccgcggacg acgcggtcgc cgcggcccag cgggacctgg
       61 ccaccgccac ggccacggtc aactccatcc agtcgaccat cgccggcctg gacgccgaca
      121 tcgccgccct ggagcaggag ctggccacca cgctggaccc ggtgcgcctg gtgcagctcc
      181 ccggcgagat cgccgccaag gtcaacagcc gtgacgccgc cgtggccagc ctggccagcg
      241 ccgaggcggc ccgcgcctcc gccgcctccg tgctcagcgc caccgagacc tccgccgtcg
      301 ccaagcgcgg cgcggccgcc accgcccgcg cggcgctgac cgcggcggag tcggccatgg
      361 cgagcgccgt gaccgcggcc gacagcgccg cgacagcgct gtccaaagcc atcagccagg
      421 ccgacgcggc ggtggccgcc gccgcggccg ccgacttggc cgtggccacg gcggcccagc
      481 aggccgacgc cctgcaggct cgcctgcgca cgctgctgca ggcggtgtcg gacctgctgg
      541 agggcatgcc gccgctgccg gtcctggagc aggagctgga cgaggcgctg cgcgccgcgc
      601 cgatcgtgtc catcggcggg ttggcgctgg agaccacggt gacggccagc cccgccggca
      661 gcagcaacct cgtgcactgc aaggtcgagc gcgtgaccgt cctgggcctg tcgcccgagg
      721 tggaggagtg caccgacctg atcgccatgg agaaggaggc ggtccgccag gtcaacgagc
      781 tgctgcagag cctgggcgcg gtgggcgtgc aaggcgtcca gctcctcggc cccggcggca
      841 cgtggagtca gccggggccc gtcgggccgg acggcaaggc gcgctccgag gggcagatgg
      901 ccgcgctgac gctgtccatc cctgaggtga gcctggacgg cgtcgccgcg aacctgctgg
      961 cgaaggtggg gccgatcctg gaggcgatca ggtcgcggcc cgcctcggcg atcatcgtgc
     1021 cgaccatccg ccccgcggac ggcaaggact tcgtccagct cgcggcttcc gggccgcgga
     1081 tggaggcggg gtcgcgcacc ctcggcgacc agatccaggt gctgtccgac gagctggaca
     1141 acgtgcgccc gcccgacgag ctgcagaacc tgtccaccgg ctccgtccac gccgtggtgg
     1201 cccaggccaa gaccgtcgcc gagttcgaca ccacgcggat ggacgaccag gacacctccg
     1261 acccgacctc cgtctgcggc ggcgacccgg tgtgcctggc gcagtgcgcg gccggcaact
     1321 gcccggggtc ggcggccggc gacgacccgg ccgccgcggg cggcgggcgc ggctccggct
     1381 ccgacagaat gggcctgcca tggtccggcg acgcgaccgg ggcgaagctg ccggtgacgg
     1441 gcgcggccgg cgggctgacg gcggtggcgc tgctggcgct gggcggcgcg ggcgcgggct
     1501 ggctgcggcg tcgcccgcgc ccgtagccgc ctcgcgtcaa cggtcggcca tggccagcca
     1561 ggtcagcacg aacgcgtaca ccgtgagcat gaagccgagc acggtcccgt aggcccagtt
     1621 gaatatgcgg ttgtcctgct tggagatctc ctcgcggatc tgcctggtca tgtccaggcg
     1681 cagcatctcg aggtcgtgct ggcggagttc ggtcggcatc acacaccccc gtgggtcgcg
     1741 gtttgcggga agcacgcgaa cgtaacccga cgtggcgcgg ctcgccgaaa tttcggcctt
     1801 ccacctgtgg atagcgcgcg ggcccccgca gttgctagcc ccagcggttg acggcgacga
     1861 tcagcgcggc cagcgcgagc gtgctccgga gatgggtgac gttcagcttg tcgagcttct
     1921 cgtgcaccga tgcgaagccc cgctccacgc ggtccgagag ccggtcgatg cggttgtcga
     1981 gacgcttgat gtcgtccttg ctcgcggctt cctcctgggc cacggtcatc gcctcctcgc
     2041 tttcgttcgg tcgtgttcgt gccgccggcg cggccggcgg gcttagcccc agcggttgac
     2101 ggcgaggatc agcgcggcca gcgcgagcgt cgagcggagc tggttggtgc tcaaccgctc
     2161 gatcttgccc tggagttcgg cctggagttc gtcgatccgg cggtgcaccg ccgcgaagcc
     2221 ccgctccacg cgatccgaga gccggtcgat gcggttgtcc agccgcctga tgtcgtcctt
     2281 ggtcgcggct tcctcttgcg ccaccgtcat ctcctcctcg ctttcgtccg gtcttctcca
     2341 gtagtcggct agccccaacg gttgacggcg acgatcagcg cggccagtgc gagcgtcgag
     2401 cgcaggtgat tgatgttgag ccgctcgatg cgttccgaaa gtcgatcgac acggtctgag
     2461 agccggtcga cacggtccga aagccggtcg atgcggccgt cgagccgctt gatgtcgtcc
     2521 ttgctcgcgg cttcctcctg cgccaccgtc atcgcctcct cgctttcggc gtcccacgat
     2581 cggagtgatt tggcgaacgt accccggagg cggcgcacac ggcggaaaac ggcctcgcgg
     2641 gctgtggaca aaaaaaccga aaggaacgtg cgcccggtat gtctgagcag tcgggaacca
     2701 acgcgtcggg gccgatgctg tcgttccggt atggccgtgc ccaccacgga cggccgggac
     2761 gacgggcggc cggggctgca catcacaccc gcacatcgag gcttgcaccc cggtcgcccg
     2821 cttcccagtt ccgccttccc gcccgcgccg cgggagccgt cctgtggaaa ggtttgcgga
     2881 accggctccc cccgccgggc gttcccctat catgaccacg ctccggcggg gtggtcccgg
     2941 cacgccgcag atgccgtctc ggcacctggc tgggttccca gaagttgacg attcgcgcgt
     3001 accgattcgc ccgcgttggc cttgacgcgg tgcggcctcg gctacggtaa gcgacattgc
     3061 cgttggcgcg ttgccagtgg tgagcaagcg ggaagggggc agttgtggcc ttggagtcgg
     3121 ctcgtggaat ggcgcgggcc cggcgaacgg tgctggcggg cgtgctcacg ctcggcctgg
     3181 tcctgtcgct ggcgccgggt gccgccgccg agcactacga aggcacgttc gaggtccagg
     3241 gcgccacgcc cacggaggcc tccgtgtcct tcagccggct gaccttcccc ggcgggtccg
     3301 ccgaggcc
//
LOCUS       DATMKC010000273         3953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_293543, whole genome shotgun sequence.
ACCESSION   DATMKC010000273 DATMKC010000000
VERSION     DATMKC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293543"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VNU01_08070"
     CDS             complement(<1..1026)
                     /locus_tag="VNU01_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH32613.1"
                     /translation="MRRLVALVAGLILLAVPAASARSVPTGTLDPLSGLQWGHEQLRL
                     AEAWGSATGVGQTIAIIDSGVDLDHPDLAGQIAGGATFVGCADAPDGCGDGDIDSGER
                     RAGDGHPHGTHVAGIVAAEAGNGEGIAGVAPDARLLAVKALNGAGEGTFEEVAAGVRW
                     AADNGADVVNLSLGALPGAQAFTLLGVEDPLLEAIDDAIAAGVVVIAAAGNEFASVCA
                     EPAFGSGALCVSATDRLEQRPLYSNFPLDPDGRAVAAPGGAALLRCQDDIVSTVPADR
                     GGVCTEIEGTPGYDFFAGTSMAAPHVAGVAALLLELGLDNAAVVDTLMATARNPLSGG
                     RGEWSPLY"
     gene            complement(1171..2040)
                     /locus_tag="VNU01_08075"
     CDS             complement(1171..2040)
                     /locus_tag="VNU01_08075"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH32614.1"
                     /translation="MERPGPRLRRLLDVWGLVPGFAVPHEHVRLAAADGVELAGSYLP
                     GPAPGGPAVALLHGFGGHHRKPRLALLAERLTAFGGVLAVDLRGHGRSGGRCTLGDRE
                     ELDALAALAWLRRRGHDWVGLLGLSMGGAAVVRGAGAAAARGPARPDAVCVISTAARW
                     GLRTSPEMTRIERLPVWTAGRAAVRGLLRTRVARGWDFPDEPLLRVARIAPAPLLIVH
                     GMDDHYFGPDQAELLYANAAEPRTLWLEPPGFGHAEDGLTPAFCDRLAAAFAEVRAHG
                     AWPERFTDRSGRA"
     gene            complement(2088..2552)
                     /locus_tag="VNU01_08080"
     CDS             complement(2088..2552)
                     /locus_tag="VNU01_08080"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HWH32615.1"
                     /translation="MEAYEVWLFLHVAAAILWVGGSTALGIMMGRLQKAQAGPALGAV
                     GAQMEFFSKAFFMPASLLAVVSGFGLVLSRDAWNFEDLWIAVGIAGFLLTLVVGMAVL
                     GPTAKKISTLGAERGPADPEVAALRERMKRFSGVNLVILWILVLDMVFKPGA"
     gene            complement(2663..3148)
                     /locus_tag="VNU01_08085"
     CDS             complement(2663..3148)
                     /locus_tag="VNU01_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWH32616.1"
                     /translation="MLTRFDPLRDVERMFSSLASDRLAAAMPMDVCRRGDELIVDMDL
                     PGVDRDAISLTVERGVLQVHARREARWREDDQVLVAERPMGEVTRRLALGDGLDLSRV
                     DASYDAGVLRLRIPVAEEAKPHRIPVRLGGEAHAVEANVARAGVAQSDETRREPAGTS
                     A"
     gene            3294..>3953
                     /locus_tag="VNU01_08090"
     CDS             3294..>3953
                     /locus_tag="VNU01_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH32617.1"
                     /translation="MDGMRADVLVAGGGLGGVAAALAAADHGARVVLTEETDWLGGQL
                     TSQAVPPDEHPWIESFGCTASYRRLRDGVREHYRRWYPLTPNARDLAVLNPGAGLVST
                     LCVEPRAALAVLEAMLAPHRGAGRLRVLARHRPVAAHADGDHVRGVTLRDLDGGGEVH
                     VEATYVLDATETGELLPLAGCEFVEGFEPARDTGEPHAPASAQPRNHQAVTWCFVLSH
                     HD"
BASE COUNT          519 a         1546 c         1450 g          438 t
ORIGIN      
        1 gtacagcggc gaccactcgc cgcgcccgcc gctcagcggg ttgcgcgcgg tggccatgag
       61 cgtgtccacg acggccgcgt tgtccaggcc cagctccagc agcagcgcgg cgacgccggc
      121 gacgtgcggc gcggccatcg acgtcccggc gaagaagtcg tagccgggcg tgccctcgat
      181 ctccgtgcag acgccgccgc ggtcggccgg cacggtggag acgatgtcgt cctggcagcg
      241 cagcagggcg gcgccgccgg gagcggccac ggcgcgcccg tcggggtcca gcgggaagtt
      301 ggagtacagc gggcgctgct ccaagcggtc ggtggcggac acgcacaggg cgccggagcc
      361 gaacgcgggc tccgcgcaca cggacgcgaa ctcgttgccc gcggcggcga tgaccaccac
      421 gcccgcggcg atggcgtcgt cgatggcctc cagcagcggg tcctccacgc ccagcaacgt
      481 gaacgcctgc gcgcccggca gcgcccccag gctcaggttg acgacgtcgg cgccgttgtc
      541 cgccgcccag cgcacgccgg cggcgacctc ctcgaacgtg ccctcgcccg cgccgttgag
      601 cgccttgacg gcgagcagtc gcgcgtccgg cgccacaccg gcgatgccct cgccgttgcc
      661 ggcctccgcg gcgacgatcc cggccacgtg cgtgccgtgg ggatgcccgt caccggcgcg
      721 gcgctcaccg gagtcgatgt cgccgtcgcc gcagccgtcc ggcgcgtccg cgcagccgac
      781 gaaggtcgcc ccgccggcga tctggccggc caggtccggg tggtccaggt ccacgccgct
      841 gtcgatgatc gcaatcgtct ggccgacccc cgttgcgctg ccccaggcct cggccaggcg
      901 cagctgctcg tgcccccact gcaggcccga cagcgggtcg agcgtgcccg tgggcacgct
      961 ccgcgcggag gcggccggca cggccaggag gatcagtccg gccacgaggg cgacgagacg
     1021 gcgcatgggg cggctccttt tccggaccag cccgccgggc ggcggccacc gatgtccaga
     1081 tgatggggat atatacccaa tagccatatc cggctagtcc cctgggtgca acccgcggtc
     1141 cgtggccgga aacacccagc agggatgcgc tcaggctcgg ccgctgcggt cggtgaagcg
     1201 ctccggccag gcgccgtgcg cgcgcacctc ggcgaacgcc gcggcgaggc ggtcgcagaa
     1261 cgccggggtc aggccgtcct cggcgtggcc gaaccccggc ggctccagcc acagcgtcct
     1321 gggctcggcg gcgttggcgt acagcagctc cgcctggtca gggccgaagt agtggtcgtc
     1381 catgccgtgc acgatcagca gtggcgcggg cgcgatgcgg gccacccgca gcagcggctc
     1441 gtccgggaag tcccagccgc gggcgacgcg ggtgcgcagc aggccgcgca cggccgcgcg
     1501 gccggcggtc cacaccggga ggcgttcgat gcgcgtcatc tccgggctgg tgcgcaggcc
     1561 ccagcgcgcg gccgtgctga tgacgcagac cgcgtcgggg cgggccggcc cgcgcgcggc
     1621 ggcggccccg gctccgcgca ccaccgccgc tccgcccatc gacagcccca gcagcccgac
     1681 ccagtcgtgg ccgcggcgcc gcagccacgc cagggccgcc agcgcgtcga gctcctcgcg
     1741 gtcgccgagc gtgcagcgcc ccccggagcg gccgtggccg cgcagatcca ccgcgagcac
     1801 gccgccgaag gcggtcaggc gctcggccag cagcgccagg cgcggcttgc ggtggtggcc
     1861 gccgaagccg tgcagcagcg ccaccgccgg accgcccggg gccggaccgg gcaggtagct
     1921 gcccgcgagc tcgaccccgt cggcggcggc cagccggacg tgctcgtggg gcacggcgaa
     1981 ccccggcacc aagccccaca cgtccagcag gcgccgcagc cgcgggcctg gccgctccac
     2041 ggcggacagg ttcgtccgcc ggccggcgcg ccgccccacc accgcggtca ggcgccgggc
     2101 ttgaacacca tgtcgagcac gaggatccac aggatcacca ggttcacccc ggagaagcgc
     2161 ttcatgcgct cccgcagggc ggccacctcc gggtccgccg ggccgcgctc cgcgcccagc
     2221 gtggagatct tcttggcggt gggcccgagc acggccatgc ccaccaccag ggtgagcagg
     2281 aacccggcga tgccgacggc gatccacagg tcctcgaagt tccaggcgtc gcggctcagc
     2341 accagcccga agccggacac gacggccagc agcgaggcgg gcatgaagaa cgccttgctg
     2401 aagaactcca tctgcgcgcc gaccgccccc agcgccggac cggcctgcgc cttctgcagg
     2461 cgccccatca tgatgccgag cgccgtgctg ccgcccaccc acaggatggc ggcggcgacg
     2521 tgcaggaaca gccagacctc gtacgcctcc acggacctac ctccttcggc ccctgccgac
     2581 cgggggctgc caggccgatc ctagaggcgc gccgtccgcg ggcgccgcag cgcgcacggc
     2641 ccgcggacgg cgtcgcgtcc gctcaggctg acgtcccggc gggctcccgc ctggtctcgt
     2701 cggactgcgc cacgcccgct cgggccacgt tggcctccac ggcgtgggcc tcgccgccca
     2761 gccgcacggg gatgcggtga ggcttggcct cctcggcgac cgggatgcgc agccgcagca
     2821 cgccggcgtc gtagctggcg tccaccctgg acaggtccag gccgtcgccc agcgcgagcc
     2881 gcctggtcac ctcccccatg ggccgctcgg cgaccagcac ctggtcgtcc tcgcgccagc
     2941 gcgcctcccg gcgggcgtgc acctgcagca ccccgcgctc gacggtgagc gagatcgcgt
     3001 cgcgatccac gccgggcagg tccatgtcga cgatcagctc gtccccgcgg cggcacacgt
     3061 ccatcggcat cgcggccgcc aggcggtccg aggccagcga ggagaacatc cgctccacgt
     3121 cacggagcgg gtcgaagcgc gtgagcatgg tcaccacctc ccaaaacact ctcgccacaa
     3181 gcgcttcggg cacggcgtcc cgtgaagggc cgccgtgcac cagatgattc ccacacgggc
     3241 ggattttacg cccccctggc gcccgcacgc cgcgggcggg tagaccgggg gccatggacg
     3301 gcatgcgcgc ggacgtgctg gtggcaggcg gcggcctggg gggcgtcgcc gccgcgctcg
     3361 cggcggccga ccacggcgcg cgcgtggtgc tcaccgagga gaccgactgg ctgggcgggc
     3421 agctcaccag ccaggcggtg ccgccggacg agcatccctg gatcgagtcg ttcggctgca
     3481 ccgcgtccta ccggcgcctg cgcgacggcg tccgggagca ctaccgccgc tggtacccgc
     3541 tgacgccgaa cgcgcgggac ctggcggtgc tgaaccccgg cgcgggactg gtctcgacgc
     3601 tgtgcgtgga gccgcgcgcc gccctggcgg tgctcgaggc gatgctcgcg ccgcaccgcg
     3661 gcgccggacg gctgcgcgtg ctggcgcgcc accgtcccgt ggccgcgcac gccgacggcg
     3721 accacgtccg cggcgtgacc ctgcgcgacc tggacggcgg cggcgaggtc cacgtggagg
     3781 cgacctacgt gctggacgcc accgagaccg gcgagctgct gccgctggcg gggtgcgagt
     3841 tcgtggaggg cttcgagccg gcgcgcgaca ccggcgagcc ccacgcgccg gccagcgccc
     3901 agccgcgcaa ccaccaggcg gtgacgtggt gcttcgtgct cagccaccac gac
//
LOCUS       DATMKC010000274         7215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_55463, whole genome shotgun sequence.
ACCESSION   DATMKC010000274 DATMKC010000000
VERSION     DATMKC010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55463"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VNU01_08095"
     CDS             complement(<1..686)
                     /locus_tag="VNU01_08095"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWH32618.1"
                     /translation="MATLLHWRSDPPPLRRPILLTALEGFVDAGGAAGTAAMFLSHRW
                     QSEVVAEFDRDSFVDYRARRPTVLVDAGELRRVDWPGIELYTASVDGPHDAVLLLGVE
                     PDMRWHAFCDEVARACRLLGVEAAIGLGAYPAAVPHTRPVRIMRATNAVAGDLVPEAI
                     PIPGYNGPVGLNSALQGALAEHGIPAVGLWAEVPHYIAGAPNPGSALALVNIVSATLQ
                     TTVDTTELEAA"
     gene            complement(748..1155)
                     /locus_tag="VNU01_08100"
     CDS             complement(748..1155)
                     /locus_tag="VNU01_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32619.1"
                     /translation="MSPYASSHVRTSYWDGPSRWINTALLVIAGFIGLDTLLRLLGAN
                     PENGVVSFVRAVAGVFLAPFRNMFPEQQYLLTALIALLGWSLLAGLILAVVRNVGPDR
                     EVVHREQRVDEPVHPEAQPPREHVADPDPTRRL"
     gene            complement(1152..1574)
                     /locus_tag="VNU01_08105"
     CDS             complement(1152..1574)
                     /locus_tag="VNU01_08105"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWH32620.1"
                     /translation="MDPNRSRLPAIPRAFLRRCPWCGGRGVFASWFELKPVCPTCGYR
                     FEREEGYWVGAMVVVTGLIYLVFAVLMLVVIAATHPDVPWTPLLVVSLALMVVVPVLA
                     YPLAKLLWIAVDLLVHHARGEVPDGAFTREGSQGIPEA"
     gene            1668..3161
                     /locus_tag="VNU01_08110"
     CDS             1668..3161
                     /locus_tag="VNU01_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWH32621.1"
                     /translation="MTDDPMSHDAMDDDARYRAVTARDPRFDGLFFVAVGSTGIYCRP
                     SCPAMTPKRANVTFHRTAAAAQQAGYRACKRCRPDAVPGSPEWDVRGDVVGRAMRLIE
                     DGVVDRDGVGALAARLGYSVRHLNRVLTEELGAGALALARARRAQTARVLLETTDLPV
                     ATVAFAAGFSSIRQCNETVRAVFDLSPTQLRRAASATPAPATAGMVPLRLPFRRPYDV
                     DRLLAFFTRRCADGLEESDGTTYRRSLPLPHGAGVVEVSAADADKGWVSCRLHLEDVR
                     DLATAVRRTRRLLDLDADSVAVDDALGSDNVLGALVAARPGLRVPGAVDGDELAVRVV
                     LGQQVSIAGAVTLAALLTARYGKPLGRPVGGVTHLFPTAAALAEAADEDLAMPQARRR
                     ALRGLCRALAEGEVVLDGSADREELGARLLALPGIGPWTVASIRMRALGDPDVFLAGD
                     LGVRYALRRLGRPEDPKAADALAGRWRPWRSYALQHLWALLNEGDEA"
     gene            3158..3646
                     /locus_tag="VNU01_08115"
     CDS             3158..3646
                     /locus_tag="VNU01_08115"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWH32622.1"
                     /translation="MSVCWTSMDTPVGRLVLTSDGAGLTGLTMEPQEGRRPVGAAHRA
                     DDVLREAVSQLDEYFAGRREVFDLPRSAPGTVFQRTVWEELVKVPFGETVSYGELARR
                     IGRPTAVRAVARANATNPLGIVVPCHRVIGGDGTLTGYAGGLERKRWLLAHEGALLPV
                     GD"
     gene            complement(3643..3942)
                     /locus_tag="VNU01_08120"
     CDS             complement(3643..3942)
                     /locus_tag="VNU01_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HWH32623.1"
                     /translation="MPVYVVATVTIHDRAGYDRYAMGFLPTLKCTGGRLLASDESPEV
                     LEGQFTASKVNLIEFPDREAFQRWYTSDAYQAIMGQRLEASHADIVLLRGFELPR"
     gene            4090..4383
                     /locus_tag="VNU01_08125"
     CDS             4090..4383
                     /locus_tag="VNU01_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWH32624.1"
                     /translation="MTVLAKVEAHTRPDTIDALRDLLAEILPDTRAFDGCLGLETWQN
                     QDDPRHFILTERWEARADHEKYLAWRMETGVLGQIVALLDGAPDISYHDEVAV"
     gene            4460..4552
                     /locus_tag="VNU01_08130"
     tRNA            4460..4552
                     /locus_tag="VNU01_08130"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4495..4497,aa:Sec,seq:tca)
     gene            complement(4650..6362)
                     /locus_tag="VNU01_08135"
     CDS             complement(4650..6362)
                     /locus_tag="VNU01_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32625.1"
                     /translation="MHHPVRRFARALLTLSAVLMLLLPAVPAVADGDDDHEVEVAGLE
                     LKAAPAVVKAGHPVLIKAKYDVDEPECPPAADAPADDSDAPAVDPDDPAGDPDGGDET
                     VETLDAHETDDTDDIDEADDADDVDEADEADEADDADDCTPEAPADDTDGTDGTDGTD
                     GTDETVESLSAHGQGQGRGKSAEAGQGRGRGGNACTSVEFVIDFGDGSEPQAVTGGGH
                     PHKMKAKAHVRHVYAEEGDYAVTVTAIPQGGTAVTATVTVQVGPGSARLSGRDRFETG
                     TDLSEEAFPEDGSASAVVLARADGFADALASATLAALEDAPVLLTSTDQLPDSVLAEI
                     QRVLAPGGTVYILGGTSAISDDVVTVLEGLGYTVERISGTDRVDTALAVAETLVAAGE
                     DIDEVVLANAGNFPDALSGAASAADRGAPVLLTPATVLDPRVREFLAGLPDGTKVTVA
                     GGAKVISDEVIAELEALGLDVQRRSGKDRFATSAVIAAEEFPDATSVVIATGKTFPDA
                     LAGAAHAGRIKAPVLLVGDTLPEDVRAYLEAGAGRINLVYVLGGHSAVPAEVMAQIQA
                     ILGL"
     regulatory      complement(6382..6468)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6571..>7215
                     /locus_tag="VNU01_08140"
     CDS             6571..>7215
                     /locus_tag="VNU01_08140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWH32626.1"
                     /translation="MERVLADRYRLRHRIGGGGMGAVFEAEDVVLERTVAAKVLPGVG
                     TGDREFLREARALSRLRHHGIVGLYDAGSDRGDAYLILELVSGQTLRERLDAGPLDAQ
                     EAGRVGHELAEALGHAHRLGVVHRDLSPSNVLLDRSGRVRLTDFGIARLSEATATSAS
                     EVWGTPAYMAPEQVAGEAVGPPADVYALGLLLLEALTGRRAFPGAPRESGFARLH"
BASE COUNT          941 a         2611 c         2720 g          943 t
ORIGIN      
        1 gcggcctcca gctccgtggt gtcgaccgtg gtctgcaggg tcgccgacac gatgttcacc
       61 agcgccaggg cactgcccgg attcggggcg cccgcgatgt agtgcggcac ctccgcccac
      121 agccccacgg ccgggatgcc gtgctcggcg agcgcgccct gcagcgcgct gttcagcccc
      181 accggcccgt tgtagccggg gatcgggatg gcctcgggga ccaggtcgcc ggccacggcg
      241 ttggtcgcgc gcatgatgcg caccggccgg gtgtgcggca cggcggccgg gtaggcgccg
      301 aggccgatgg cggcctccac gccgagcagg cggcacgcgc gggcgacctc gtcacagaac
      361 gcgtgccagc gcatgtccgg ctccacgccc aacagcagca cggcgtcgtg cggaccgtcc
      421 acggacgcgg tgtacagctc gatgccgggc cagtcgacgc ggcgcagctc gccggcgtcg
      481 acgagcacgg tggggcgccg ggcgcggtag tcgacgaagc tgtcgcggtc gaactcggcc
      541 acgacctccg actgccagcg gtgcgacagg aacatcgccg ccgtccccgc ggccccgccg
      601 gcgtccacga agccctccag cgcggtcagc aggatcggcc gccgcagggg cggcgggtcg
      661 ctgcgccagt gcaacagggt cgccatgccg ccaggctact tgccgcggcc ggcggcgcgg
      721 aacgcggccg acgcggcggc tggcgcccta caggcggcgg gtgggatcgg ggtccgcgac
      781 gtgctcgcgc gggggctgcg cctctgggtg gacgggctcg tccacgcgct gctcgcggtg
      841 cacgacctcg cggtccgggc ccacgttgcg caccacggcc aggatcaggc cggccagcag
      901 gctccagccc agcagggcga tgagcgcggt cagcaggtac tgctgctccg ggaacatgtt
      961 gcggaagggg gccaggaaca ccccggcgac cgcgcgcacg aacgacacca ccccgttctc
     1021 ggggttggcg cccagcaggc gcagcagcgt gtcgagaccg atgaagcccg cgatcacgag
     1081 cagcgccgtg ttgatccagc gcgacgggcc gtcccagtag ctcgtgcgga catggctgga
     1141 ggcgtagggg ctcatgcctc gggtatgccc tgggagccct cccgcgtaaa cgcgccgtcg
     1201 ggcacctcgc cgcgcgcgtg gtgcaccagc aggtccaccg cgatccacag cagcttcgcc
     1261 agcgggtagg ccaggaccgg caccaccacc atgagggcca gggacaccac caacagcggc
     1321 gtccacggca cgtccgggtg cgtggcggcg atcacgacga gcatcagcac ggcgaacacc
     1381 aggtagatga ggccggtcac cacgaccatc gccccgaccc agtagccctc ctcgcgctcg
     1441 aagcggtagc cgcacgtggg gcagaccggc ttcagttcga accagctcgc gaagacgccg
     1501 cgcccgccgc accacgggca gcggcgcagg aacgcgcgcg ggatcgcggg caggcggctg
     1561 cggttggggt ccatgcccac gacggtaccg ctccgggaaa ccgtggacgg gagcggaatg
     1621 gccgatttcc gccagcggct gtcgcagcca gcggcgacca tgggaccgtg accgacgacc
     1681 cgatgagcca cgacgcgatg gacgacgacg cgcgctaccg cgccgtgacc gcccgggatc
     1741 cccgcttcga cgggctgttc ttcgtcgccg tgggatcgac cggcatctac tgccgcccga
     1801 gctgtccggc gatgaccccc aagcgcgcca acgtcacgtt ccaccgcacc gccgccgcgg
     1861 cgcagcaggc cggctaccgc gcgtgcaagc gctgccggcc ggacgccgtg cccggctcgc
     1921 cggaatggga cgtgcgcggc gacgtggtcg gccgggccat gcgcctgatc gaggacggcg
     1981 tcgtggaccg cgacggcgtc ggcgcgctgg ccgcccgcct gggctacagc gtccgccacc
     2041 tgaaccgcgt gctgaccgag gagctgggag cgggcgccct cgcgctggcg cgggcgcggc
     2101 gcgcgcagac ggcgcgcgtg ctgctggaga ccaccgacct gcccgtggcc accgtcgcct
     2161 tcgccgccgg cttctccagc atccgccagt gcaacgagac cgtgcgcgcc gtgttcgacc
     2221 tgtcgcccac gcagctgcgc cgtgccgcga gcgccacacc cgcgccggcg accgccggga
     2281 tggtgccgct gcgcctgccg ttccggcgcc cgtacgacgt ggaccgcctg ctcgcgttct
     2341 tcacgcgccg ctgcgccgac ggcctggagg agtccgacgg cacgacgtac cgccgctcgc
     2401 tgcccctgcc gcacggcgcc ggggtcgtgg aggtgtccgc cgcggacgcg gacaagggct
     2461 gggtgtcctg ccgcctgcac ctggaggacg tgcgcgacct ggccacggcc gtgcgccgga
     2521 cgcggcggct gctggacctc gacgccgaca gcgtcgccgt ggacgacgcg ctcggcagcg
     2581 acaacgtcct gggagcgctg gtcgcggccc gcccgggcct gcgcgtgccg ggcgccgtgg
     2641 acggcgacga gctggcggtg cgcgtggtgc tgggccagca ggtctccatc gcgggcgccg
     2701 tgacgctggc ggcactgctc accgcgcgct acggcaagcc gctcgggcgg ccggtcggcg
     2761 gcgtgacgca cctgttcccc accgccgcgg cgctggccga ggcggccgac gaggacctcg
     2821 ccatgccgca ggcgcgccgc cgcgcgctgc gcggcctgtg ccgggcactg gccgagggcg
     2881 aggtcgtcct ggacggcagc gcggaccgcg aggagctcgg cgcccggctc ctggcgctgc
     2941 cgggcatcgg accgtggacc gtggcgtcga tccgcatgcg cgccctgggc gacccggacg
     3001 tgttcctggc cggcgacctc ggcgtgcgct acgcgctgcg gcggctcggc cggccggagg
     3061 accccaaggc ggcggacgca ttggcgggcc gctggcggcc atggcggtcc tacgccctgc
     3121 agcacctgtg ggcgctgctg aacgaaggag acgaggcatg agcgtgtgct ggacgagtat
     3181 ggacaccccg gtcggcaggc tggtgctcac cagcgacggg gccggcctga ccggcctgac
     3241 gatggagccc caggagggcc gacggcccgt cggcgccgcg caccgcgccg acgacgtgct
     3301 gcgagaggcg gtgagccagc tcgacgagta cttcgctggg cggcgcgagg tgttcgacct
     3361 gccgcggtcg gctcccggca ccgtcttcca gcgcacggtg tgggaggaac tggtgaaggt
     3421 ccccttcggc gagacggtca gctacgggga gctcgcgcgg cgcatcggcc ggcccacggc
     3481 cgtgcgtgcc gtcgcgcgcg cgaacgccac caacccgctg gggatcgtgg tcccctgcca
     3541 ccgggtgatc ggcggcgacg ggacgctcac cggctacgcg ggcggcctgg agcgcaagcg
     3601 ctggctgctc gcgcacgagg gcgcgctgct gccggtgggt gactagcgcg gcagctcgaa
     3661 gccgcgcagc aggacgatgt cggcgtggct cgcctccagc cgctgcccca tgatcgcctg
     3721 gtaggcgtcg gaggtgtacc agcgctggaa cgcctcgcgg tcggggaact cgatcaggtt
     3781 caccttgctc gcggtgaact gcccctccag cacctcgggg gactcgtcgg atgcgagcag
     3841 ccgcccgcct gtgcacttca gcgtgggcag gaagcccatc gcgtagcggt catagccggc
     3901 ccggtcgtga atcgtcaccg tggccaccac gtacaccggc atcgatgccc cttcctccgg
     3961 acgtccgtcc gcccgcggac gcacaccgca gcctaagtcc tccacgcccg tcgaccggac
     4021 ggtggccgcg gcctaggctg cgagcccggc gtcgttcggc ggcgaaggag accgacggga
     4081 ggagcgggca tgacggttct tgcgaaggtg gaggcgcaca cgcgtccgga cacgatcgac
     4141 gcactgcggg acctgctggc ggagatcctg ccggacacgc gcgcgttcga cggctgcctg
     4201 ggactggaga cgtggcagaa ccaggacgac ccccggcact tcatcctgac ggagcgctgg
     4261 gaagcgcggg ccgaccacga gaagtacctg gcgtggcgca tggagacggg cgtgctaggc
     4321 cagatcgtcg cgctgctcga cggcgcgccg gacatcagct accacgacga ggtcgccgtc
     4381 tagcgagcga cccgcgacgg gggcctgccg ggaccgtgag gacgcctgcg tccgcgaccc
     4441 taggatgggc cgcgcacagg gaggcgtctg ggttcctggt gggccccacg gtcttcaaaa
     4501 ccgatgaggg cggctcggcc gtcctggcgg gttcgattcc cgtccgcctc cgctcccgca
     4561 gacggaaccg gggtgtgtcg agctggtcaa ccacacgttg actcagtcga cacaccccgg
     4621 ttggagcggg tgtccgcgag aggtggcggc tacaggccca ggatggcctg gatctgcgcc
     4681 atgacctcag ccggcacggc gctgtggcca cccagcacgt agaccaggtt gatccggccg
     4741 gcccctgcct ccaggtaggc gcgaacgtcc tccggcagcg tgtcgccgac cagcagcacc
     4801 ggagccttga tccgcccggc gtgcgccgcg ccggcgagcg cgtccgggaa ggtcttgccg
     4861 gtggcgatga ccaccgacgt ggcgtccggg aactcctcgg cggcgatcac cgccgaggtc
     4921 gcgaagcggt ccttgccgct gcgccgctgc acgtccaggc ccagcgcctc cagctccgcg
     4981 atgacctcgt cggagatcac cttggcgccg ccggcgaccg tgaccttggt gccgtccggc
     5041 aggccggcca ggaactcgcg cacgcgcggg tccaggacgg tcgccggggt gagcagcaca
     5101 ggggcgccgc ggtcggcggc gctggccgcg ccggacagcg cgtcggggaa gttgccggcg
     5161 ttggccagca cgacctcgtc gatgtcctca ccggcggcga ccagcgtctc ggccacggcc
     5221 agggcggtgt ccacgcgatc cgtgccggag atgcgctcga ccgtgtagcc gagcccctcc
     5281 agcaccgtca cgacgtcgtc ggagatggcc gacgtgccgc ccaggatgta cacggtgccg
     5341 cccggggcca gcacgcgctg gatctccgcg agcaccgagt ccgggagctg gtcggtcgag
     5401 gtcagcagca ccggcgcgtc ctccagcgcc gccagcgtgg ccgaggccag ggcgtcggcg
     5461 aacccgtcgg cccgggcgag cacgaccgcg gaagcgctgc cgtcctcggg gaaggcctcc
     5521 tcggacaggt cggtgcccgt ctcgaagcgg tcacggccgg acaggcgcgc gctgccgggg
     5581 cccacctgca cggtgaccgt cgcggtgacg gccgtgccgc cctgcgggat ggccgtgacg
     5641 gtgacggcgt agtcgccctc ctcggcgtag acgtgacgca cgtgcgcctt ggccttcatc
     5701 ttgtgggggt ggccgccacc ggtgacggcc tgcggttcgc tgccgtcacc gaagtcgatg
     5761 acgaactcca cggacgtgca ggcgttgccg ccccgcccgc gcccctggcc ggcctcggcg
     5821 ctcttgccgc gaccctggcc ctggccgtgg gcgctcagcg actccaccgt ctcgtcggtg
     5881 ccgtcggtgc cgtcggtgcc gtcggtgccg tcggtgtcgt cggccggcgc ctcgggggtg
     5941 cagtcgtcgg cgtcgtccgc ctcgtccgcc tcgtccgcct cgtccacgtc gtcggcgtcg
     6001 tccgcctcgt cgatgtcgtc ggtgtcgtcg gtctcgtggg cgtccagagt ctcgacggtc
     6061 tcgtcgccgc cgtccgggtc gccggccggg tcatcagggt cgacggccgg agcgtcggag
     6121 tcgtcggcgg gagcgtccgc ggcgggcggg cactccggct cgtcgacgtc gtacttggcc
     6181 ttgatgagga ccgggtgacc ggccttcacg acggcgggcg cggccttcag ctccaggccc
     6241 gcgacctcga cctcgtggtc gtcgtcgccg tcggcgaccg cggggaccgc cggcagcagc
     6301 agcatgagca cggcggaaag ggtgagcagc gcgcgcgcga agcggcgcac agggtggtgc
     6361 atgacgtact cccttcggtg aaggggcaac ccggctgacc gccttggggg aaggcggtcc
     6421 cgtggctttg cgagccccgc cttgcggcgg ggggtgccct tagacgtctt gtcggccgat
     6481 acagcgtacg ggacgagtca aaggatacgg acaaaaggaa tcgattttcc ggccatgaac
     6541 cggcgccccc gcgggcatgg tggccgggtc gtggaaagag tgctcgctga ccgttaccgg
     6601 ctccgccacc gcatcggcgg tggcggcatg ggtgccgtgt tcgaagccga ggacgtggtg
     6661 ctggagcgca cggtcgcggc gaaggtgctg cccggggtgg gcaccggtga ccgcgagttc
     6721 ctgcgcgagg cgcgtgcgct gtcccggctg cggcaccacg ggatcgtggg gctgtacgac
     6781 gccggctccg accgggggga cgcctacctg atcctggagc tggtgtcggg ccagaccctg
     6841 cgcgaacgcc tcgacgccgg ccccttggac gcgcaggagg ccgggcgcgt gggtcacgag
     6901 ctggccgagg cgctcggcca cgcgcaccgc ctgggtgtgg tgcaccgcga cctgtcgccc
     6961 tccaacgtgc tgctggaccg cagcggccgc gtgcgcctga ccgacttcgg catcgcgcgg
     7021 ctgtccgagg ccacggccac gtcggcgtcc gaggtctggg gcactcccgc ctacatggcg
     7081 cccgagcagg tcgccggcga ggcggtcggc cccccggcgg acgtgtacgc cctgggcctg
     7141 ctgttgctgg aggcgctgac cggccggcgg gcgtttcccg gcgcgcccag ggagtccggc
     7201 ttcgccaggc tgcac
//
LOCUS       DATMKC010000275         3543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_5105, whole genome shotgun sequence.
ACCESSION   DATMKC010000275 DATMKC010000000
VERSION     DATMKC010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5105"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VNU01_08145"
     CDS             <1..637
                     /locus_tag="VNU01_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32627.1"
                     /translation="LGPLGAMCRQYLRAPSVVVELDWQRGAEPTAAARQHLVAVLRQA
                     TQADVTLAGGSEIPGEAEVSSPDSLRALAAAHRDTFSTQDRAVMYVLSVRGRFQEESA
                     LGVAYRASEFAIFPDLIGGLSALLGGRAAIERAVLVHEAGHLLCLVNIGYSSAFDRED
                     PDHPKHSSDRSSAMHWAIETSAIAQLFSGPPPDTFTAQDLADLEGLRSGRY"
     gene            704..2251
                     /gene="guaA"
                     /locus_tag="VNU01_08150"
     CDS             704..2251
                     /gene="guaA"
                     /locus_tag="VNU01_08150"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWH32628.1"
                     /translation="MSAPAEDFDSVLVLDFGAQYAQVIARRVRECHVYSEIVPCSITA
                     EEVRARRPKGLILSGGPKSVYAENAPTVDPELFELGVPVLGICYGQQLMAATLGGEVA
                     RTGGSEFGKTEVEVDPTSVLLRDQPRDQTVWMSHNDAVVRAPDGFRVVGSSPGAPVAA
                     FEDPERGFFGVQYHPEVTHTPRGMDVLRSFLAHTGAKTSWTAHSVIEAAVEAIRAQVR
                     DAKVLCALSGGVDSAVAAALVHEAIGAQLTCVFVDHGLLRAGEAEQVQETFGDQYSTK
                     LVTVKAADRFLEKLAGVTDPETKRKIIGTEFIRVFEDAAREHAGDAAFLVQGTLYPDV
                     IESGHGTAATIKSHHNVGGLPEDMAFDLVEPLRWLFKDEVRRVGEELGLPPEIVWRQP
                     FPGPGLAVRIIGEVTGERLELLRAADRIVLEELRRAGLEREIWQSFAVLPAIRSVGVQ
                     GDERTYGHPIVVRAVTSEDAMTADFARIPWEVLEKISTRVINEVPGINRVAYDITSKP
                     PGTIEWE"
     gene            complement(2256..2720)
                     /locus_tag="VNU01_08155"
     CDS             complement(2256..2720)
                     /locus_tag="VNU01_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWH32629.1"
                     /translation="MGLNIKDLRIESDGFADGGAMPDKYSKYHDNVVPPLRWSGVPEG
                     TRQLALICHDPDAPLPHGWTHWVVYGIAPDVTQITEDGPVPGVEGTTDYGEVGWGGPM
                     PPPGHGTHHYFFWLYALDTEIDAEPGLTRAQLLERIQDHVIEQARIVGTYEK"
     gene            complement(2711..2866)
                     /locus_tag="VNU01_08160"
     CDS             complement(2711..2866)
                     /locus_tag="VNU01_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32630.1"
                     /translation="MRILRVRPILRASRVLQRRLGDAHRLGKAGGAATFVVARRRRVQ
                     QEEVQWD"
     gene            complement(2887..>3543)
                     /locus_tag="VNU01_08165"
     CDS             complement(2887..>3543)
                     /locus_tag="VNU01_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH32631.1"
                     /translation="ARMLANRARAVVVSVGYRLAPEHPYPAALEDALAAVTWAADEAD
                     ALGADGGRLAVAGDSSGGNLAAAACLWARDHGGPTIAAQCLVYPVTDLEPDDGSWREF
                     GQGANLDAGDMEWFNANYAADPRRRAEPYASPLRAPDLSGLPPAIVATASHDILRDQG
                     EAYARRLEGAGVAVFARRYEGLVHGFFRFSEVSPAARRANEEICARLRALLHGGGAPG
                     "
BASE COUNT          472 a         1281 c         1304 g          486 t
ORIGIN      
        1 cctcggcccg ctgggcgcca tgtgccggca gtacctgcgc gcgccgagcg tcgtggtgga
       61 actcgactgg cagcgcggcg ccgagccgac cgccgccgcg cgccagcacc tcgtcgccgt
      121 cctgcggcag gcgacgcagg ccgacgtgac gctggccggc gggtccgaga tcccgggcga
      181 ggccgaggtg tcctccccgg acagcctcag ggcgctggcc gccgcccacc gcgacacctt
      241 ctccacccag gaccgggccg tcatgtacgt gctcagcgtg cgcgggcggt tccaggagga
      301 gagcgcgctg ggcgtggcct accgcgccag cgagttcgcg atcttccccg acctgatcgg
      361 cgggctgtcg gcgctgctcg gcgggcgggc ggcgatcgag cgcgcggtgc tcgtgcacga
      421 ggccggccac ctgctgtgcc tggtgaacat cggctactcc tcggcgttcg accgcgagga
      481 ccccgaccac ccgaagcact cgtctgaccg ctcgtcggcc atgcactggg cgatcgagac
      541 ctcggccatc gcgcagctgt tctccgggcc gccgccggac accttcacgg cgcaggatct
      601 ggccgacctc gaggggctgc gctcgggccg gtactgagac aggccggggt gtaccgtttg
      661 cggccagagc cgtccagcag ccgtgcagca ggggagtagg accgtgagcg cacccgcgga
      721 ggacttcgac agcgtcctcg tgctcgactt cggcgcccag tacgcgcagg tgatcgcgcg
      781 gcgcgtgcgg gagtgccacg tgtactcgga gatcgtgccg tgctcgatca ccgcggagga
      841 ggtcagggcc cgccgtccca agggcctgat cctgtccggc gggcccaagt cggtgtacgc
      901 cgagaacgcg ccgacggtgg acccggagct gttcgagctg ggcgtgccgg tgctgggcat
      961 ctgctacggc cagcagctca tggccgccac gctgggcggc gaggtggcgc gcacgggcgg
     1021 cagcgagttc ggcaagaccg aggtcgaggt cgacccgacc agcgtgctgc tgcgcgacca
     1081 gccgcgcgac cagaccgtct ggatgtcgca caacgacgcc gtggtgcgcg cccccgacgg
     1141 cttccgcgtg gtcggctcct cgccgggcgc gcccgtcgcc gccttcgagg atcccgagcg
     1201 gggtttcttc ggtgtgcagt accacccgga ggtgacgcac acgccgcgcg gcatggacgt
     1261 gctgcgcagc ttcctcgccc acaccggtgc gaaaacgtcg tggaccgcgc actccgtgat
     1321 cgaggcggcg gtggaggcga tccgcgccca ggtccgcgat gccaaggtgc tgtgcgcgct
     1381 gtcgggcggg gtggactccg cggtggccgc cgcgctggtc cacgaggcca tcggcgcgca
     1441 gctcacctgc gtgttcgtgg accacgggct gctgcgcgcg ggcgaggcgg agcaggtgca
     1501 ggagaccttc ggcgaccagt actccaccaa gctggtgacg gtgaaggccg ccgaccgctt
     1561 cctggagaag ctggccggcg tgaccgaccc ggagaccaag cgcaagatca tcggcaccga
     1621 gttcatccgc gtgttcgagg acgcggcgcg cgagcacgcc ggcgacgccg cgttcctggt
     1681 gcagggcacg ctgtacccgg acgtgatcga gtccgggcac ggcaccgcgg ccaccatcaa
     1741 gagccaccac aacgtgggcg ggctgccgga ggacatggcg ttcgacctgg tggagccgct
     1801 gcgctggctg ttcaaggacg aggtgcgccg cgtcggcgag gagctggggc tgccgccgga
     1861 gatcgtgtgg cgccagccgt tccccggccc gggcctggcg gtgcgcatca tcggcgaggt
     1921 gaccggggag cgcctggagc tgctgcgcgc cgcggaccgc atcgtgctgg aggaactgcg
     1981 ccgggcgggg ctggagcgcg agatctggca gagcttcgcg gtgctgcccg ccatccgctc
     2041 cgtcggcgtg cagggcgacg agcgcaccta cggccatccc atcgtcgtgc gcgccgtcac
     2101 gtcagaggac gccatgaccg ccgacttcgc ccgcatcccg tgggaggtgc tggagaagat
     2161 ctccacgcgg gtgatcaacg aggtgcccgg cattaaccgc gtggcctacg acatcacgtc
     2221 caagcccccc ggcaccatcg agtgggagtg accgtctact tctcgtaggt gccgacgatg
     2281 cgggcctgct cgatcacgtg gtcttggatg cgctccagca gttgcgcgcg agtgagcccg
     2341 ggctcggcgt cgatctcggt gtccagcgcg tagagccaga agaagtagtg gtgggtgccg
     2401 tggccgggtg gcggcatggg cccgccccag ccgacctcgc cgtagtcggt cgtcccctcg
     2461 acgccgggca cggggccgtc ctcggtgatc tgcgtcacgt ccggcgcgat gccgtacacc
     2521 acccagtgcg tccagccgtg cggcagcggc gcgtccgggt cgtggcagat cagcgccagc
     2581 tggcgggtac cctccggcac accgctccag cgcaggggcg gcaccacgtt gtcgtggtac
     2641 ttggagtact tgtccggcat ggcgccaccg tcggcgaagc cgtcgctctc gatgcgcaga
     2701 tccttgatgt tcagtcccac tgcacttcct cctgttgcac tctccgccgc cgcgccacca
     2761 cgaaggtcgc ggcgcccccg gccttgccca gacgatgcgc gtcgcccagg cgccgctgca
     2821 gcacgcggct ggcccgcagg atcggccgga cgcgcaggat gcgcaaggcc cccagacagc
     2881 gcgcgcctac ccaggcgccc cgccgccgtg cagcagtgcc cggaggcgcg cgcagatctc
     2941 ctcgttcgcc cgccgggccg cgggggagac ctcgctgaag cggaagaagc cgtgcaccag
     3001 cccctcgtag cgccgcgcga acaccgccac gccggccccc tccagccgcc tggcgtaggc
     3061 ctcgccctgg tcgcgcagga tgtcgtggct ggcggtcgcc acgatcgcgg ggggcagccc
     3121 ggacaggtcc ggcgcgcgca gcggcgacgc gtacggctcg gcgcgccgcc gggggtccgc
     3181 cgcgtagttc gcgttgaacc actccatgtc gcccgcgtcg agattcgcgc cctggccgaa
     3241 ctcccgccat gagccgtcgt cgggctccag gtccgtgacg gggtacacca ggcactgcgc
     3301 cgcgatcgtg gggccaccgt ggtcgcgtgc ccacaggcag gcggcggcgg ccaggttgcc
     3361 gcccgagctg tcacccgcga cggcgagccg cccgccgtcg gcaccgagcg cgtcggcctc
     3421 gtcggccgcc cacgtcaccg cggccagcgc gtcctcgagc gccgccgggt aggggtgctc
     3481 gggcgccagg cggtagccga ccgacacgac gacggcgcgg gcccggttgg ccagcatccg
     3541 ggc
//
LOCUS       DATMKC010000276         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_126969, whole genome shotgun sequence.
ACCESSION   DATMKC010000276 DATMKC010000000
VERSION     DATMKC010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126969"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VNU01_08170"
     CDS             <1..1641
                     /locus_tag="VNU01_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWH32632.1"
                     /translation="VSDPVRLRPRRGDWPRGTGQVRADLLAGLTVGIVALPLALAFGV
                     SSGMGAAAGLVTAIVAGAVAAAFGGSRFQVSGPTGAMTVVLIPVVARHGAEAVLLVGV
                     LAGLFLVVLAGLRVGRFVGFIPTPVVEGFTLGIALIIALQQVPAALGVDKPEAERTLA
                     LVWQAAEAFLRAPAAPSLVLTGVVVALMVVLPRLLRAVPASLVAVALATLAAEVLRLD
                     VPRIGALPSGFAVPALGDLPWAQLDTLVGPAIAIAALAAIESLLSATVADGMSGGERH
                     DPDRELFGQGLANAASCLVGGMPATGAIARTAVNIRAGAQTRLSALAHAGVVAGVVLG
                     LGGVVALIPLAALAGVLLVTAARMVEFGSLRAIVRTTRSDALVLVATAAATLLFDLVV
                     AVEVGIAVAGVLALRHIARSTLMERLEAAPLAEDPEGLLQRRVAVYRFDGSLFFGAAH
                     RFLLELTSVSDVDAVVLRLSGVRTMDATGAALLGDVIERLEHRGVAVLLSGARPQHES
                     LLRRAGVLERLAHERHLFAHADDAVRHAIVHAERKRAA"
     gene            1738..>2657
                     /locus_tag="VNU01_08175"
     CDS             1738..>2657
                     /locus_tag="VNU01_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWH32633.1"
                     /translation="MPQPGLAASPDAAAPARPTRPADGFRRLAIVTTALTLVLIAIGG
                     LVRATDSGLACPDWPRCYGMWVPPADVNIWIEHTHRLVAGVVATLIWVLAAWALRRHR
                     HRRDIVVPVLLAWALVHVQALLGALVVWHLLRAELVTAHLGMAMIVVACLIAAAVAVN
                     VPPGEGRWTPMAKAHAVVAALAFAQILVGGHVTGIGASLAFTDFPTMGGTMLPEISTE
                     REAFHVLHRALAFVLLGAAIWLAARAARYRTAAMQTGTWTRAQRLVVKLPMWAATLIV
                     LQIGLGVANLWTRASYLTVTPHLAVASWIWA"
BASE COUNT          243 a          973 c         1037 g          404 t
ORIGIN      
        1 gtgagcgatc cggtgcggct ccggccccgg cgtggcgact ggccgcgcgg caccgggcag
       61 gtgcgcgccg acctgctcgc cggtctcacc gtgggcatcg tcgcgctgcc gctggccctg
      121 gccttcggcg tgtcctcggg catgggcgcc gcggccgggc tggtcaccgc gatcgtggcc
      181 ggggcggtgg ccgcggcctt cggcggctcc cgcttccagg tgtcgggccc cacgggcgcg
      241 atgaccgtgg tgctcatccc cgtggtcgcg cgccacgggg ccgaggcggt cctgctggtc
      301 ggcgtgctgg cggggctgtt cctggtggtg ctggccgggc tgcgggtcgg ccggttcgtg
      361 gggttcatcc cgacgccggt ggtggagggc ttcacgctgg gcatcgcgct catcatcgcg
      421 ctgcagcagg tgcccgccgc gctcggggtc gacaagccgg aggcggagcg caccctggcg
      481 ctggtgtggc aggccgccga ggcgttcctg cgggcgcccg cggccccgtc gctggtgctg
      541 accggcgtgg tcgtggcgct catggtggtc ctgccgcgac tgttgcgcgc cgtgccggcg
      601 tccctggtgg cggtggccct cgcgacgctc gcggccgagg tgctgcgcct ggacgtgccc
      661 cgcatcggcg cgctgccctc gggtttcgcg gtgcccgccc tcggcgacct cccgtgggcg
      721 cagcttgaca cgctggtcgg accggccatc gccatcgcgg ccctggccgc catcgagagc
      781 ctgctgtcgg ccacggtcgc cgacggcatg agcgggggcg agcggcacga cccggaccgc
      841 gagctgttcg ggcagggcct ggcgaacgcg gcgtcctgcc tggtgggcgg catgccggcc
      901 acgggcgcca ttgcgcgcac ggccgtcaac atccgcgccg gcgcgcagac gcggctgtcg
      961 gcgctggcgc acgccggcgt cgtggccggg gtggtgctgg gcctgggcgg cgtcgtggcc
     1021 ctgatcccgt tggcggcgct ggccggcgtg ctgctggtca ccgcggcgcg catggtcgag
     1081 ttcggctcgc tccgggcgat cgtgcgcacc acccgttccg acgcgctcgt gctcgtcgcg
     1141 accgcagccg cgacgctgct gttcgacctc gtggtcgccg tggaggtcgg catcgccgtg
     1201 gccggcgtgc tggccttgcg ccacatcgcg cgcagcacgc tgatggagcg gctggaggcg
     1261 gccccgctcg cggaggatcc cgaggggctg ctgcagcgcc gcgtggcggt gtaccggttc
     1321 gacggcagcc tgttcttcgg cgccgcccac cggttcctgc tggagctcac cagcgtgtcg
     1381 gacgtggacg cggtggtgct gcggctgtcc ggggtgcgca ccatggacgc caccggggcg
     1441 gccctgctgg gggatgtgat cgagcgcctg gagcaccggg gtgtcgcggt gctgctgtcg
     1501 ggcgcccggc cccagcacga gtcgctgctg cgacgggcgg gcgtgctgga gcgcctggca
     1561 cacgagcggc acctgttcgc gcacgccgac gacgcggtgc gccacgccat cgtccacgcg
     1621 gagcggaagc gggccgcgtg aggttcggcc ctccggcgcc tcgcggctac actcgacggc
     1681 gacccgctcc cgcacgggcg ttgccctgcg cgttcgacat tccgacgagg tgaactcgtg
     1741 ccccagcccg gcctcgcggc gtcccccgac gccgccgcgc ccgcacgccc cacgcggccg
     1801 gccgacggct tccgccgcct cgccatcgtc accaccgccc tcacgctcgt gctcatcgcg
     1861 atcggcggcc tggtgcgcgc caccgactcc ggccttgcct gcccggactg gccgcgctgc
     1921 tacggcatgt gggtgccccc cgcggacgtg aacatctgga tcgagcacac gcaccggctc
     1981 gtggccggcg tggtggccac gctgatctgg gtgctggcgg cgtgggcgct gcgccgccat
     2041 cgccaccgcc gggacatcgt cgtgcccgtg ctgctggcct gggcgctggt gcacgtgcag
     2101 gcgctgctgg gcgcactggt ggtgtggcac ctgctgcggg cggagctggt gaccgcccac
     2161 ctgggcatgg cgatgatcgt ggtcgcctgc ctcatcgcgg ccgccgtcgc ggtcaacgtg
     2221 ccacccggcg agggccgctg gaccccgatg gccaaggcgc acgcggtcgt ggcggcgctg
     2281 gcgttcgcgc agatcctggt gggaggccac gtcaccggca tcggcgcgag cctggccttc
     2341 accgacttcc ccacgatggg cggcacgatg ctgcccgaga tctccaccga gcgggaggcg
     2401 ttccacgtgc tgcaccgggc cctggcgttc gtgctcctgg gcgcggcgat ctggctggcc
     2461 gcgcgggcgg cgcgctaccg caccgccgcg atgcagaccg gcacgtggac gcgggcgcag
     2521 cgcctggtgg tgaagctgcc gatgtgggcg gccacgctga tcgtgctgca gatcggcctc
     2581 ggcgtggcga acctgtggac ccgcgcctcc tacctgacgg tcacgccgca cctggccgtg
     2641 gcgagctgga tctgggc
//
LOCUS       DATMKC010000277         6001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_233689, whole genome shotgun sequence.
ACCESSION   DATMKC010000277 DATMKC010000000
VERSION     DATMKC010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233689"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1952
                     /gene="uvrC"
                     /locus_tag="VNU01_08180"
     CDS             24..1952
                     /gene="uvrC"
                     /locus_tag="VNU01_08180"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWH32634.1"
                     /translation="MRNPALAFRPDPGTIPVEPGCYLWRDGNGRVVYVGKAKSLRARL
                     SNYFGDWGGIATRTRAMLEAARSVEWIVTASEVEALHLEYNLIKRHRPRYNVRYTDDK
                     SYPYLAITVQDEVPRATVRRNPRKDGTRYFGPYAHAYAIRETLDLLLRVFPVRTCSDG
                     VFARARRADQPCLLHHIGRCAAPCTLKVSVDEHRDLVEDLMAFLDGDTADTLDALEAR
                     MRQEAAKQNFESAARIRDQIASVRRALERQVMATERPEDFIAVHFHADDLEAAFQAFF
                     VRRGRVVGRKGWHVDKVEDLDDAELVGRFVLELAMEVGEELPREVLVPVLPPDWDVLE
                     ELLTEQRNRPDADGQGARTGQVKLKVPRRGDRRAFLETVRGNAVEAFQQSKLKRAKDF
                     NARSQALQELQEALDLREPPLRIECYDISTLQGSDNVASMVVMEDGLPRKSEYRRFKI
                     RWQQGQDDFAAMHEVITRRFRKLQEEESQPITERAFSYRPNLVIVDGGKGQLAAARRA
                     LDELGEHDVELCSLAKRFEELYLPGRADPVALPRGSEALYLVQRVRDEAHRFAVGYHR
                     SLRGKRMTASALDELSGVGPARRKALLKHFGSVKRMREASPEELAEVSGISGTLAQRI
                     YDQLHRPPARGPEQEEPA"
     gene            1949..2809
                     /gene="rapZ"
                     /locus_tag="VNU01_08185"
     CDS             1949..2809
                     /gene="rapZ"
                     /locus_tag="VNU01_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWH32635.1"
                     /translation="MSPQLAIITGLSGAGRTTAAKVLEDLGYYVVDNLPPSLIGSIVD
                     LATAPGSTVDRVALVLDVRGRQFFGDLRVTLSDLKERGAQPRVLFLEADDDTLVRRYE
                     AARRAHPLSGKDRVVDGIARERELLADLRAEADLVVDTTGLNVHELRDRLVDAFGEGA
                     HDGMVVNVVSFGFKNGTPRDADIVLDVRFLPNPHWVEELRPYTGLDEPVRTYVMERPE
                     TAEFIERLARLLDVMVPAFVKEGKHYLTVAVGCTGGKHRSVVLAEEVAEHIRSHGVDV
                     QVDHRDRLKE"
     gene            2813..3745
                     /locus_tag="VNU01_08190"
     CDS             2813..3745
                     /locus_tag="VNU01_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HWH32636.1"
                     /translation="MAAASAPRRVVALGGGHGLSRALAALRALDVEPTAVITVADDGG
                     SSGRLRQELGVIPPGDLRMALLTLARNRELAGALSHRFAAGALEGHALGNLLLVALAE
                     RAGGDFLAGLAEAERLLDVAGRVLPASLEPVSLHGRIGGRDVSGQVRLATAEAPVERV
                     WADPVPPACAAAVEALEQADAVVLGPGSLFTSIIAALLVPDLAKALTAVQAPVLYIAN
                     VRTQVGETSGLDLAAHVDALFAHVPGLRLDAVVAHDGPVADAAGEPIPPVLDDPRVGR
                     LVTADVVARDAEGRPGWGHDADRLAAALAPLLPR"
     gene            3756..4688
                     /gene="whiA"
                     /locus_tag="VNU01_08195"
     CDS             3756..4688
                     /gene="whiA"
                     /locus_tag="VNU01_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HWH32637.1"
                     /translation="MSFAQEMRDELAHVAVERTCCRRAETSALLRFGGALRLGSGGIG
                     WVLTTSSGAVARRTRSALDALHGVRPDVEVHQPGGLRHATAYRLAVPPPAEGLLADLG
                     LLDARRRPVDGVPQRLTRNRCCAASYVRGALMAAGSVAEPRHDPHLEIRAPGEAAALA
                     VAALTERLSGHRAGVGEHAGSWRVAIKSGTAIGTLLTQAGAHSAFLRWDGERLRRELR
                     GDANRIANADRANLQRTVTASARQIEAAERLLASADWDELAPELQEAALARLANPEAS
                     LTELGGLLDPPVGKSAVYRRLTRVVALAEALERA"
     gene            complement(4944..>6001)
                     /locus_tag="VNU01_08200"
     CDS             complement(4944..>6001)
                     /locus_tag="VNU01_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH32638.1"
                     /translation="VAERVEGLPLELLLSLACRMTGADRRMLVTAGEVLADMPATALL
                     FREGALSWGQVRAVVAAVRRLPVEDRAQIDARIAASAERHDGIDAYDPDQLVWAVDQA
                     VERLRDARATERAEARRRESSFVSVQAGLDGGVRLYAELDAVRGATVLNALDAAAARP
                     ASAAPEPGASTAAGNGHEEVTAAAAAWSSTPRARQYADALESVCADWLGGDAHRPARP
                     LVVAHVDVSQLRSRADGTVELAVRGGLPRVSAATLEALAASGDVRAVVFDGARPPASV
                     VIGVGTLVAATRLDASAALAFAERRGAVAATRARGKHHPITWSIASMAASTTRRTCSR
                     CPGAITRWCTGTAGGCE"
BASE COUNT          766 a         2211 c         2284 g          740 t
ORIGIN      
        1 ggagggcacc taccatgggt gcgatgcgca atcccgccct ggcgttccgc cccgatcccg
       61 gcaccatccc ggtggagccg ggctgctacc tgtggcggga cggcaacggc cgcgtcgtct
      121 acgtcggcaa ggccaagagc ctgcgggcgc gcctgtccaa ctacttcggg gactggggcg
      181 gcatcgccac ccgcacgcgc gcgatgctgg aggcggcgcg ctccgtggag tggatcgtca
      241 cggccagcga ggtcgaggcg ctgcacctgg agtacaacct catcaagcgc caccgcccgc
      301 gctacaacgt ccgctacacc gacgacaaga gctacccgta cctggcgatc accgtgcagg
      361 acgaggttcc ccgggcgacg gtgcggcgca acccgcgcaa ggacggcacc cgctacttcg
      421 ggccgtacgc ccacgcctac gccatccgcg agaccctgga cctgctgctg cgcgtcttcc
      481 cggtgcgcac ctgctccgac ggcgtgttcg cccgggcgcg ccgcgccgac cagccgtgcc
      541 tgctgcacca catcggccgc tgcgccgcgc cctgcacgct gaaggtctcg gtcgacgagc
      601 accgcgacct ggtcgaggac ctgatggcgt tcctggacgg ggacaccgcc gacacgctcg
      661 acgcgctcga ggcgcgcatg cggcaggagg ccgcgaagca gaacttcgag tccgcggcgc
      721 gcatccgcga ccagatcgcg tcggtgcgcc gcgcgctgga gcgccaggtg atggccaccg
      781 agcggccgga ggacttcatc gccgtgcact tccacgccga cgacctggag gcggccttcc
      841 aggcgttctt cgtgcggcgc ggccgcgtgg tcggccgcaa gggctggcac gtcgacaagg
      901 tggaggacct ggacgacgcc gagctggtcg gccgcttcgt cctggagctg gccatggagg
      961 tgggcgagga gctgccgcgg gaggtcctcg tgccggtgct gccgccggac tgggacgtcc
     1021 tggaggagct gctcaccgag cagcgcaacc ggccggacgc cgacgggcag ggcgcgcgca
     1081 ccggccaggt caagctgaag gtcccccggc gcggggaccg ccgggcgttc ctggagacgg
     1141 tgcgcggcaa cgccgtcgag gcgttccagc agtccaagct caagcgcgcc aaggacttca
     1201 acgcgcgcag ccaggcgctg caggagttgc aggaggcgct ggacctgcgc gagccgccgc
     1261 tgcgcatcga gtgctacgac atctccacgc tccagggcag cgacaacgtg gcctccatgg
     1321 tggtcatgga ggacggcctg ccgcgcaaga gcgagtaccg gcgcttcaag atccgctggc
     1381 agcagggcca ggacgacttc gcggcgatgc acgaggtgat cacgcgccgc ttccgcaagc
     1441 tgcaggagga ggagtcccag ccgatcaccg agcgcgcgtt ctcctaccgc ccgaatctgg
     1501 tgatcgtcga cggcggcaag ggccagctcg cggcggcgcg gcgcgcgctg gacgagctgg
     1561 gcgagcacga cgtcgagctg tgctcgctcg ccaagcgctt cgaggagctg tacctgccgg
     1621 ggcgggccga cccggtggcg ctgccgcgcg gcagcgaggc gctgtacctg gtgcagcgcg
     1681 tgcgcgacga ggcgcaccgc ttcgcggtcg gctaccaccg ctccctgcgc ggcaagcgca
     1741 tgacggcctc ggcgctggac gagctgtccg gcgtggggcc cgcccggcgc aaggcgctgc
     1801 tgaagcattt cggctccgtg aagcgcatgc gggaggcgag cccggaggag ctggcggagg
     1861 tgagcggcat cagcggcacg ctggcgcagc gcatctacga tcagctgcac cggccgcccg
     1921 cccgggggcc ggagcaggag gagcccgcat gagcccgcag ctcgcgatca tcaccggctt
     1981 gtccggcgcg ggccggacga cggcggcgaa ggtcctggag gacctgggct actacgtggt
     2041 cgacaacctg ccgccgtcgc tcatcggcag catcgtcgac ctggccaccg ccccgggctc
     2101 cacggtggac cgcgtcgccc tggtgctgga cgtgcgcggc cggcagttct tcggcgacct
     2161 gcgcgtcacc ctgagcgacc tcaaggagcg cggcgcccag ccgcgggtgc tgttcctgga
     2221 ggccgacgac gacacgctgg tgcgccgcta cgaggcggcg cgccgcgccc acccgctgtc
     2281 cggcaaggac cgcgtggtcg acggcatcgc gcgcgagcgc gagctgctgg ccgacctgcg
     2341 cgcggaggcg gacctggtgg tggacaccac cggcctgaac gtgcacgagc tgcgcgaccg
     2401 cctggtcgac gcgttcggcg agggcgcgca cgacggcatg gtggtcaacg tcgtgagctt
     2461 cggcttcaag aacggcacgc cgcgcgacgc cgacatcgtc ctggacgtgc gcttcctgcc
     2521 gaacccgcac tgggtggagg agctgcggcc ctacaccggc ctggacgagc cggtgcgcac
     2581 ctacgtcatg gagcggcccg agaccgcgga gttcatcgag cgcctggccc ggctgctgga
     2641 cgtgatggtt ccggccttcg tcaaggaggg caagcactat ctcaccgtgg cggtcggctg
     2701 caccggcggc aagcaccgtt ccgtggtgct cgcggaagag gtcgccgagc acatccgcag
     2761 ccacggcgtg gacgtgcagg tcgaccatcg cgaccggctc aaggagtagg cagtggcagc
     2821 ggcgtcggca ccgcggcggg tggtcgcgct gggcggcggc cacgggctgt cgcgcgccct
     2881 cgcggccctg cgcgccctgg acgtcgagcc gaccgcggtg atcaccgtgg ccgacgacgg
     2941 cgggtccagc gggcgcctgc gccaggagct gggcgtcatc cctcccggcg acctgcgcat
     3001 ggcgctgctc acgctggcgc gcaaccgtga gctggcgggc gcgctgagcc accgcttcgc
     3061 cgccggggcg ctggagggcc acgccctggg caacctgttg ctggtggcgc tcgccgagcg
     3121 cgccggcggc gacttcctgg ccgggctggc cgaggccgag cgcctgctgg acgtggcggg
     3181 ccgggtgctg cccgccagtt tggagccggt gtcgttgcac gggcgcatcg gcggccgcga
     3241 cgtgtccggc caggtgcgcc tggccaccgc ggaagccccc gtggagcgcg tgtgggccga
     3301 cccggtcccg cccgcgtgcg cggcggccgt cgaggcgctg gagcaggccg acgccgtggt
     3361 gctcgggccg ggcagcctgt tcaccagcat catcgcggcc ctgctggtcc cggacctggc
     3421 gaaggcgctc accgcggtgc aggcgcccgt gctgtacatc gccaacgtgc gcacccaggt
     3481 gggcgagacc agcgggctgg acctggccgc gcacgtcgac gcgctgttcg cacacgtgcc
     3541 cggcctgcgc ctggacgccg tggtggcgca cgacggaccg gtggccgacg ccgccggcga
     3601 gccgatcccg ccggtcctgg acgacccgcg cgtcgggcgg ctggtgaccg ccgacgtcgt
     3661 ggcgcgcgac gccgaaggcc gtcccggctg gggtcatgac gccgaccgcc tggccgccgc
     3721 gctcgcgccg ctgctgccgc gctgagaggc gggccgtgag cttcgctcag gagatgcgcg
     3781 acgagctcgc gcacgtggcg gtcgagcgga cctgctgccg ccgcgcggag acgtccgcgc
     3841 tgctgcgctt cggcggcgcc ctgcggctgg gctccggcgg catcggctgg gtgctcacca
     3901 ccagctccgg ggcggtcgcg cgccgcacgc gcagcgccct ggacgccctg cacggcgtgc
     3961 ggcccgacgt ggaggtccac cagccgggcg gcctgcggca cgccacggcc taccggctgg
     4021 cggtgccgcc gccggccgag ggcctgctgg ccgacctggg gctgctggac gcgcgcaggc
     4081 ggccggtcga cggcgtgccg cagcggctca cccgcaaccg ctgctgtgcc gcgtcgtatg
     4141 tgcgcggcgc gctcatggcc gcgggcagcg tcgcggagcc ccgccacgac ccccatctgg
     4201 agatccgcgc gccgggcgag gccgccgccc tggcggtcgc cgcgctgacg gagcgcctga
     4261 gcgggcaccg cgccggcgtg ggggagcacg cgggctcgtg gcgggtcgcg atcaagtcgg
     4321 ggaccgccat cgggacgctg ctcacgcagg ccggcgcgca cagcgcgttc ctgcgctggg
     4381 acggtgagcg gctgcggcgg gagctgcgcg gcgacgccaa ccgcatcgcc aacgccgacc
     4441 gcgcgaacct gcagcgcacg gtgaccgcgt ccgcccgcca gatcgaggcg gccgaacggc
     4501 tgctcgcctc ggcggactgg gacgagctcg cacccgagtt gcaggaggcc gcgctggccc
     4561 ggctggccaa tcccgaggcg agcctgacgg aactcggcgg cctgctggac ccgccggtcg
     4621 gcaagtccgc ggtgtaccgg cgcctgacgc gcgtcgtggc gctcgccgag gcactcgagc
     4681 gcgcgtgacc gccgctgggg cgtgcgtgcc cgtgtgaggg catgccagtg gcccggccgg
     4741 cggccgggtc atctggctgg atggactgag tggcggatgc gcggtcggga ccgcaccggg
     4801 tggtcagaag ggcaggccgg tggctcggga accgtcggct tcgtcccctg gctcgtcacc
     4861 cgcctcggcg agtggcgtgc cgcgcggcag gctgcgccag gcgcggtgcc gtcgttgtgc
     4921 ggtgacgacg ccagtctccg gcgttattcg cagccgccag ccgtgccggt gcaccagcgt
     4981 gtgatggcgc cgggacagcg agagcaagtt ctccgggtgg tgctcgccgc catgctcgcg
     5041 atggaccatg tgatggggtg gtgcttgccg cgtgcacgtg tcgcggccac cgcgcctcgc
     5101 cgctcggcaa aggcgagagc agcgctcgcg tcgaggcgcg tggccgcgac caacgtgcct
     5161 acgccgatca ccactgacgc tggcggccgc gccccgtcga acacgaccgc gcgcacgtcg
     5221 ccggatgcgg ccagcgcctc cagcgtggcg gcgctgacgc gcggcaggcc accgcgcacg
     5281 gcgagttcga cggtgccgtc cgcgcgggag cggagctgcg acacgtcgac gtgcgccacc
     5341 accagcggac gcgccggccg gtgggcgtcg ccgccgagcc agtccgcaca gaccgactcc
     5401 agcgcgtcgg cgtactggcg cgccctgggc gtggaggacc atgccgccgc ggcggccgtg
     5461 acctcctcgt gcccattgcc ggccgcggtg ctcgcgccgg gctccggcgc ggccgacgcg
     5521 gggcgcgccg ccgccgcgtc cagcgcgttg agcacggtcg cgccgcgcac cgcgtcgagc
     5581 tcggcgtaca ggcgcacgcc accgtccagg cccgcctgca cggacacgaa gctcgactcg
     5641 cggcggcgcg cttcggcgcg ctcggtggcg cgcgcgtcgc gcagccgctc gacggcctgg
     5701 tcgaccgccc acacgagctg gtcggggtcg tacgcgtcga tcccgtcgtg gcgctccgcc
     5761 gacgccgcga tccgcgcgtc gatctgcgca cgatcctcca cgggcaggcg ccgcaccgcc
     5821 gcgacgacgg cccgcacctg cccccacgag agcgcgccct cgcggaacag gagcgccgtg
     5881 gcgggcatgt ccgccagcac ctccccggcg gtcacgagca tgcgccggtc cgcgcccgtc
     5941 atgcggcacg ccaggctgag cagcagctcc aacggcagcc cctcgacgcg ctcggccacg
     6001 c
//
LOCUS       DATMKC010000278         5275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_219770, whole genome shotgun sequence.
ACCESSION   DATMKC010000278 DATMKC010000000
VERSION     DATMKC010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219770"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="VNU01_08205"
     CDS             <1..1787
                     /locus_tag="VNU01_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32639.1"
                     /translation="VETIKHLEIQYEELKKLEGEASVDGLLGIASADRVRQAVRAKRA
                     ALRHDARMRAQATSLDIAKELEKARERLGDLTADLANASTGKALMDAAREALSDDLSD
                     LTLDKNAHDEVLDAARDASLDADGDVTEREVDVEQATKALEAITAGAGNAGETVEAIA
                     ARIQDKEVDVLGTVATVRNLAADLAALVPIYQGLTETLQPWPMFKIHAISYELDTRVG
                     YHGTQAKVRCGLEGVELLGEMLPLRTCDDLRGAKPMIEQELADFLALFTLERPEVELT
                     VPSGIVDDQGLVNGQRKVRTALTPLSLSISPFQLRTLTDPLMDTINARIDTLQEQVSY
                     DEIRDLARLGEPPQKLTDWVLDWPRDREWEAPSDRDQIEMASVRPARVQAAGEPRYGA
                     ASADPGNTEQDLDRELDGFQEDAGQTAGNEVGGQGTSGVDASVGGMETDVMWAGCVGC
                     VDPPCQPGEPDCPDPGPCVQGSPDCPPPCVPGNPDCPVNCRAGDLDCPDKCKPGAACT
                     GDKLKGGKGTAGDAEGADGGAGGPGNGGGGDGTGNGVDGYPLGDVVGDANLPHTGGRP
                     ATLLLGLVLLLAAGLMRRRPAAAEPTRR"
     gene            2132..2539
                     /locus_tag="VNU01_08210"
     CDS             2132..2539
                     /locus_tag="VNU01_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32640.1"
                     /translation="MIDLAGTQPRFRPSLIAAATEGRQIDRGNAGLRSMEEDFRRLGV
                     GGLNSRIAARNSHWSRTPTRRGDCAFYADLIELRNALAHGNQRQLERLRSRGVMDTVT
                     WARDRLAGLDRIARALDLVVWDHVVSNFGVEPW"
     gene            2545..2733
                     /locus_tag="VNU01_08215"
     CDS             2545..2733
                     /locus_tag="VNU01_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32641.1"
                     /translation="MADELRVGATVRVPWGIEGDVKGKILEIWGEPPAHVRVELLLEG
                     DDEPLVLLLAASTLSAAC"
     gene            complement(2738..2863)
                     /locus_tag="VNU01_08220"
     CDS             complement(2738..2863)
                     /locus_tag="VNU01_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32642.1"
                     /translation="MAREGDLERVRNEVESMFDLRRGTTEPEDLTDDAAALFGLG"
     gene            3063..3299
                     /locus_tag="VNU01_08225"
     CDS             3063..3299
                     /locus_tag="VNU01_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32643.1"
                     /translation="MPKAITIRDVPDEVRDELAARAARAGQSLQEFLRTELLELASQP
                     TIEELLSDVRARKRATGSTLAAETIVSYRDADRP"
     gene            3296..3697
                     /locus_tag="VNU01_08230"
     CDS             3296..3697
                     /locus_tag="VNU01_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWH32644.1"
                     /translation="MTQRGLVVDASVVVAAFVDSGPHGAWAEQRLSAGPLFGPHLLPV
                     EAANVLRRAELAGDITADTAALAHRDVGRLRVRLLPFAPFAGRVWELRDTLTAYDAWY
                     VAIAETLEAPLATLDARTARAPGPACAFVCP"
     gene            3740..4339
                     /locus_tag="VNU01_08235"
     CDS             3740..4339
                     /locus_tag="VNU01_08235"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH32645.1"
                     /translation="MRTDAAALLREARAAAGLTQREVAERSGVSQPHVSAYENNRRQP
                     TLPTMARLVNATGHELVLGLRERTPYRPLTLAGIAPRIAADAPGARGGRWFWVREFLR
                     GFSDDTSTTVERSGLIAEAPPQTGEQEWDAVLAALAEHLSFHRTLPCPEWVHDDRRVA
                     EHAWFLSDLPAQRAWAVETSPASFRRRNLFVLPEDLHVA"
     gene            4336..4854
                     /locus_tag="VNU01_08240"
     CDS             4336..4854
                     /locus_tag="VNU01_08240"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HWH32646.1"
                     /translation="MNPSMDRAAMQEALEELNATLKRHGVRADLYVFGGAAMVLAYGA
                     DRPTRDIDAKFEPHGAVLQAAQEVASRLDLPRSWLNDQASVYLSQQRDTGQAPVFDRS
                     HLRVQAASAEHLVAMKVLAAREHDLADLRLLIGRLGLRDAAEAEAITRRFFPDEDIPA
                     RNLMALEDLFAG"
     gene            complement(4884..>5275)
                     /locus_tag="VNU01_08245"
     CDS             complement(4884..>5275)
                     /locus_tag="VNU01_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32647.1"
                     /translation="PSRVVVLGGTGAVSEATRAALGELAPGGAERVSGDSRFSTAAAV
                     CAAGWDYPIDTVYVATGAGFADALAGVPAAAKEGAPLLLVERDAVPAPTAQELARIRP
                     ARIVILGGTSAVSEATADQLASHLRLT"
BASE COUNT          820 a         1874 c         1886 g          695 t
ORIGIN      
        1 ccgtggagac catcaagcat ctggagatcc agtacgagga gctcaagaag ctcgaggggg
       61 aggcctccgt cgacggcctg ctgggcatcg ccagcgccga ccgcgtccgc caggcggtgc
      121 gggccaagcg cgccgcgctg cgccacgacg cccgcatgcg cgcccaggcc acgtccctcg
      181 acatcgccaa ggagctggaa aaggctcgcg agcggctcgg ggacctgacc gccgacctcg
      241 ccaacgcctc caccggcaag gcgctgatgg acgcggcccg cgaggcgctc agcgacgacc
      301 tgtccgacct gaccctggac aagaacgccc acgacgaggt gctggacgcc gcgcgggacg
      361 cgtcgctgga cgcggacggt gacgtgaccg agcgcgaggt cgacgtggag caggccacca
      421 aggcgctgga ggcgatcacc gccggcgccg gcaacgccgg cgagaccgtc gaggcgatcg
      481 ccgcccgcat ccaggacaag gaggtcgacg tcctgggcac agtggccacg gtgcgcaacc
      541 tggccgcgga cctggcggcg ctggtgccga tctaccaggg cctgaccgag acgctgcagc
      601 cctggccgat gttcaagatc cacgcgatct cctacgagct ggacacgcgc gtgggctacc
      661 acggcacgca ggcgaaggtg cgctgcggcc tggagggcgt ggaactgctg ggcgagatgc
      721 tgcccctgcg cacctgcgac gacctgcgcg gcgccaagcc gatgatcgag caggagctgg
      781 ccgacttcct ggcgctgttc acgctggagc ggcccgaggt ggagctgacc gtgccgtccg
      841 gcatcgtcga cgaccagggc ctggtcaacg gccagcgcaa ggtgcgcacc gccctgacgc
      901 cgctgtcgct gtcgatctcg ccgttccagt tgcgcacgct gaccgacccg ctgatggaca
      961 ccatcaacgc gcgcatcgac acgctgcagg agcaggtcag ctacgacgag atccgggacc
     1021 tggcgcgcct gggcgagccg ccgcagaagc tgaccgactg ggtgctggac tggccccgcg
     1081 accgggagtg ggaggccccc tccgaccgcg accagatcga gatggcttcc gtccgccccg
     1141 cgcgggtcca ggcggccggg gagccgcgct acggcgccgc ctccgccgac ccgggcaaca
     1201 ccgagcagga cctggaccgc gagctggacg gcttccagga ggacgccggc cagaccgccg
     1261 gcaacgaggt cggcggccag ggcacctcgg gcgtggacgc gtccgtgggc ggcatggaga
     1321 ccgacgtcat gtgggccggc tgcgtcggct gcgtggaccc gccgtgccag ccgggtgagc
     1381 cggactgccc ggacccgggc ccgtgcgtcc agggctcgcc ggactgcccg ccgccgtgcg
     1441 tgcccggcaa cccggactgc ccggtcaact gccgggccgg cgacttggac tgccccgaca
     1501 agtgcaagcc cggcgcggcg tgcacgggcg acaagctgaa gggcggcaag ggcacggccg
     1561 gcgacgccga gggcgccgac ggcggcgccg gcggcccggg caacggcggc ggcggcgacg
     1621 ggaccggcaa cggagtcgac ggttacccgc tgggcgacgt ggtcggcgac gcgaacctgc
     1681 cgcacaccgg cggccggccc gcgacgctgc tgctcggact ggtgctgctg ctggccgcgg
     1741 gcctcatgcg ccgcaggccc gcggccgccg agcccacacg gaggtaggcg acaccgcgca
     1801 cggcttggca gtgcatcgcg gactcgcggc tgtggatatc ttcgccccac gcgcggccgg
     1861 ccctcttcgg atcccacgat tcgtccacga ttcgtgggaa gccgggggca ggccaatgtc
     1921 atatcgacag tccatcccac gcgcgcagtg gctccaggaa cgacgccaga acatcgaaac
     1981 gctggcacaa gcccacgcga ccatgagcgc agtcggaggc cctggccgtc cgcgagaggt
     2041 cggccggccc gtgggccatg cctacgtact cagagtggtc tccgaattcc aagcgttcgt
     2101 acgagacgtc catgatctga gcgccgagcg catgatcgat ctggccggta cccaaccgcg
     2161 cttccggccg tcgttgatcg cagcagcaac ggaagggcgc cagatcgatc gaggcaatgc
     2221 ggggcttcgc agcatggagg aggatttccg aaggctcggg gttggcggtc tcaactcacg
     2281 gatcgccgcc cggaattccc actggagcag aacacccacg cggcgcggcg actgcgcttt
     2341 ctacgccgac ctgatcgaac tgcgcaacgc cttggcgcac ggcaatcagc gccagctgga
     2401 gcggttgcgg agtcgcggtg tcatggacac ggtcacatgg gctcgggacc gcctggccgg
     2461 gctggacagg atcgcgagag cattggacct cgtcgtgtgg gatcatgtgg taagcaactt
     2521 cggagtcgag ccatggtgag tcagatggca gatgagcttc gcgtcggcgc cacggtgcga
     2581 gtgccttggg gcatcgaggg tgatgtcaag ggcaagatcc tcgagatatg gggcgaaccg
     2641 cccgcgcatg tgcgcgtgga gttgctgctc gagggagacg acgagccgct cgtgctgctc
     2701 ctggccgcgt ctaccctctc cgccgcctgc taggccgtca ccctaggccg aacagcgccg
     2761 cggcgtcgtc cgtcaggtcc tcgggctcgg tcgtaccgcg acgcaggtcg aacatcgact
     2821 cgacctcgtt cctgacacgc tccaggtcgc cctcgcgcgc caaccgctcc agatgctgga
     2881 cgaggtcgag gacggcggcc ccctcctggt cggccaggaa gtccgcgcgg cgcgcgggtc
     2941 gctcgccacg tagctggcga gccgctcagc gagaggtcga gaacccatag ggcccacgct
     3001 attgcaacgt gatggcaaca gggagcgttg ccgagtgctg cttctgcctg cacaatgcat
     3061 gcatgcccaa ggccatcacg atccgcgacg ttcccgacga ggtccgcgac gaactggcgg
     3121 cgcgagccgc gcgcgccggc cagtcattgc aggagttcct gcgcacggaa ctgctggagc
     3181 tggcgagcca gccgaccatc gaagagctgc tctccgacgt gcgcgcccgc aagcgcgcca
     3241 cgggctccac gctggccgca gagacgatcg tgtcctaccg ggacgctgat cgcccgtgac
     3301 ccagcggggc ctggtggtcg acgccagcgt ggttgtggcg gcgttcgtgg actcggggcc
     3361 ccacggcgcc tgggccgaac agcgcctgag cgccggcccg ctgttcgggc cgcacctgtt
     3421 gccggtggag gccgccaacg tgctgcgccg cgcggaactg gccggcgaca tcacggcaga
     3481 cacggcggcg ctcgcccatc gcgacgtcgg gaggctgcgt gtgcggctcc tgccgttcgc
     3541 ccctttcgcg ggccgcgtgt gggagttgcg cgacaccctc accgcgtacg acgcctggta
     3601 cgtcgcgatc gccgagacgt tggaggcacc gctggcgacg ctggacgccc gaacagcacg
     3661 agcccccggc cctgcgtgcg ccttcgtctg cccctgacac gtgcctgccg aatataaaca
     3721 agcaggtatg ctacggcgta tgcgaacgga cgcggccgca ctgctcaggg aggcgagagc
     3781 cgcagccggc ctgacccaga gagaggtggc cgagcgcagc ggcgtctcac agcctcatgt
     3841 gagcgcgtac gagaacaacc gccggcaacc gaccctgccg acgatggcac gcctcgtgaa
     3901 cgccaccggg cacgagttgg tcctggggct tcgcgaacgg acgccatacc ggccactgac
     3961 gctggccggg attgcaccca gaatcgccgc cgatgcgccg ggcgcccgcg gtggccgctg
     4021 gttctgggtc cgggaattcc tgcgcggctt ctccgacgac acgagcacca ccgtggagcg
     4081 gtccggcctc atcgccgagg cgccccccca gaccggtgaa caggaatggg acgcggtcct
     4141 ggccgccctc gcggagcatc tgtccttcca ccgcacgctg ccgtgccccg agtgggtgca
     4201 tgacgaccgc cgcgtcgccg agcacgcgtg gttcctctcc gacctgccgg cccagcgggc
     4261 gtgggcggtg gagacatctc ccgcgtcctt ccgccgccgc aacctgttcg tcctgcccga
     4321 ggatctccac gtcgcatgaa cccgtcgatg gatcgcgcgg cgatgcagga ggcgctcgag
     4381 gagctgaacg cgaccctcaa gcgacacggt gtgcgtgcgg acctctacgt gttcggtggg
     4441 gcggccatgg tgctggcata cggcgcggac cgtcccaccc gggacatcga cgcgaagttc
     4501 gagccgcacg gtgccgtgtt gcaggcggcg caggaagtcg ccagccggct cgacctgccg
     4561 cgaagctggc tcaacgacca ggcgtcggtc tacctgtcac agcagcggga caccggccag
     4621 gcgcccgtgt tcgaccgcag ccatttgcgg gtgcaagccg cgtcagccga gcaccttgtc
     4681 gccatgaaag tgctggcggc ccgcgaacac gacctcgccg atctccgtct gctcatcggc
     4741 cggctcgggt tgcgcgacgc cgcggaagcg gaggcgatca cgcggcgctt ctttccggac
     4801 gaggacatcc cggcgcgcaa cctcatggcg cttgaggacc tgttcgccgg ctgaggcgcc
     4861 ggcagagcat gcccgccctg cgttcaggtc aggcgcaggt ggctggcgag ctggtcggcg
     4921 gtcgcctcgg acaccgcgct ggtgccaccc aggatcacga tgcgcgccgg ccggatgcgg
     4981 gcaagctcct gcgcggtggg cgcgggcacg gcgtcgcgct ccaccagcag cagcggcgcg
     5041 ccctccttgg cggccgcggg caccccggcc agggcgtcgg cgaacccggc gccggtcgcc
     5101 acgtacaccg tgtcgatcgg gtagtcccag ccggccgcgc acacggccgc ggcggtggag
     5161 aagcggctgt cgccggagac gcgctcggcg ccgccggggg cgagctcgcc cagtgcggcg
     5221 cgcgtcgcct ccgacaccgc gccggtccct cccagcacca ccacgcgcga cgggg
//
LOCUS       DATMKC010000279         3173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_173585, whole genome shotgun sequence.
ACCESSION   DATMKC010000279 DATMKC010000000
VERSION     DATMKC010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173585"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..516
                     /gene="rpiB"
                     /locus_tag="VNU01_08250"
     CDS             64..516
                     /gene="rpiB"
                     /locus_tag="VNU01_08250"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWH32648.1"
                     /translation="MRIAIGADHAGYRLKEHLVGFLRAQGHDVDDHGTGSEESVDYPP
                     IMAAVGRAVAGGEAERGIVLGGSGQGEQIAANKVHGVRAALCNDLYTARLSREHNDAN
                     VLAMGGRIVAPGLAEEIVALWLSTGFEGGRHERRVQQIAELERQQEQR"
     gene            513..1778
                     /gene="glyA"
                     /locus_tag="VNU01_08255"
     CDS             513..1778
                     /gene="glyA"
                     /locus_tag="VNU01_08255"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWH32649.1"
                     /translation="MSQTWGPDYDVLQATDPEVAKAIADELNRQRTDLQLIASENFTS
                     PAVLAAQGSVLTNKYAEGYPGKRYYGGCEFVDVAETHAIERAKRLFGAAHANVQPHSG
                     AQANMAAYVALGVQHGDTVMAMNLAHGGHLTHGSKVNFSGKWYEIAAYGVAEGTELLD
                     MDQVREIALQARPKVIVAGATAYPRVIDFAAFRAVADEVGAKLMVDAAHFAGLVAGGE
                     HPSPVPHAHVVTFTTHKTLRGPRGGCVLSTAELAQAVDKAVFPMLQGGPQMHAVAGKA
                     VAFHEALQPSYADYAARVVANARALAEAMAAEGMRIVSGGTDIHYLLADVTPLGLTGA
                     EAERRCDAAGISLNKNAIPFDQHPPMVASGIRVGVPSVTTQGMGPEQMADVAGLIARV
                     LRNEGDIGQVRKEVHELTARFPVYPAPAA"
     gene            1795..2931
                     /locus_tag="VNU01_08260"
     CDS             1795..2931
                     /locus_tag="VNU01_08260"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWH32650.1"
                     /translation="MIEPSPLDFLLVFLAALATVLVTTPLAGRLAYRLGAVDQPGERR
                     VHERPTPRLGGLAIFMGLLAAIVVGARLPGFERVFTHTSEPEAILLASLAVVVLGVLD
                     DVRGVSAPAKLAGQILSAGVLVLFGVTLLFVYIPGNPGSVVSLSTDLGAIVTVLAVVA
                     MINTVNLVDGLDGLAAGIVLIAATALLSYVQFSDPTALGLPSAASLVLTAVIGATLGF
                     LVFNFHPASIFMGDTGAMLLGLLLASAGVSAIGGTVQPSRGAFAAFSVPVLVPVLVLA
                     VPFLDTAWAILRRLRAGQPVFSPDKRHLHHRLIEIGHSHRRAVLIMYYWSALLAFATV
                     GVGLLPLRVVAAVVGAGALLAVAVAVLPRLAGRGSSGSGTPSVR"
BASE COUNT          424 a         1151 c         1141 g          457 t
ORIGIN      
        1 ggcgatgcgc atgcgtctac tcctccgccg cggcgcccgg cgccgcggcg gaggagtaga
       61 cgcatgcgca tcgccatcgg cgccgaccac gccggctacc gcctcaagga gcacctcgtg
      121 gggttcctgc gcgcgcaggg ccacgacgtg gacgaccacg gcacgggctc cgaggaatcc
      181 gtcgactacc cgccgatcat ggcggcggtg ggccgcgcgg tcgccggcgg cgaggcggag
      241 cgcggtatcg tgctgggcgg cagcggccag ggcgagcaga tcgccgccaa caaggtccac
      301 ggcgtgcgcg cggcgctgtg caacgacctc tacacggcgc ggctgtccag ggagcacaac
      361 gacgccaacg tgctggccat ggggggacgc atcgtcgccc cgggcctcgc ggaggagatc
      421 gtggcgctgt ggctgtccac cggcttcgag ggcggccggc acgagcgtcg cgtgcagcag
      481 atcgccgaac tggaacgaca gcaggagcag cgatgagcca gacgtggggc ccggactacg
      541 acgtcctcca ggccaccgac ccagaggtgg ccaaggccat cgccgacgag ctcaaccggc
      601 agcgcacgga cctgcagctc atcgccagcg agaacttcac ctcgccggcc gtgctggcgg
      661 cgcagggcag cgtgctgacc aacaagtacg ccgagggcta cccgggcaag cgctactacg
      721 gcggctgcga gttcgtcgac gtggcggaga cgcacgcgat cgagcgcgcc aagcgcctgt
      781 tcggcgcggc gcacgccaac gtgcagcccc actccggcgc gcaggcgaac atggcggcct
      841 acgtggcgct cggcgtccag cacggtgaca ccgtgatggc catgaacctc gcgcacggcg
      901 gccacctcac ccacggctcc aaggtgaact tcagcggcaa gtggtacgag atcgccgcct
      961 acggcgtcgc cgagggcacc gagctgctcg acatggacca ggtgcgcgag atcgccctgc
     1021 aggcgcggcc gaaggtgatc gtggcgggcg cgacggccta cccccgcgtc atcgacttcg
     1081 cggcgttccg cgcggtcgcc gacgaggtcg gcgccaagct gatggtggac gcggcgcact
     1141 tcgccgggct ggtggcgggc ggcgagcacc ccagtcccgt gccgcacgcc cacgtcgtga
     1201 cgttcaccac gcacaagacc ctgcgcgggc cccgcggcgg ctgcgtgctg tccaccgcgg
     1261 agctggcgca ggcggtggac aaggccgtgt tccccatgct gcagggcggc ccgcagatgc
     1321 acgcggtggc cggcaaggcg gtcgcgttcc acgaggcgct gcagccgtcc tacgccgact
     1381 acgccgcccg tgtggtggcc aacgcccgcg cgctcgcgga ggccatggcc gccgagggca
     1441 tgcgcatcgt ctcgggcggc acggacatcc actacctgct cgcggacgtg acgccgctgg
     1501 gcctgaccgg cgcggaggcg gagcgccgct gcgacgcggc cggcatctcg ctcaacaaga
     1561 acgccatccc cttcgaccag cacccgccga tggtcgcctc cggcatccgc gtgggcgtgc
     1621 cgtcggtgac cacgcagggc atgggcccgg agcagatggc cgacgtggcg ggcctgatcg
     1681 cgcgggtgct gcgcaacgag ggcgacatcg gccaggtgcg caaggaagtg cacgagctga
     1741 ccgcgcgctt ccccgtctat ccggccccgg ccgcctgaga gcgcgccgcg gcgcatgatc
     1801 gagccgagcc cgcttgactt cctgctggtc ttcctggcgg cgctggccac cgtcctggtg
     1861 accacgccgc tggcaggccg gctggcctac cggctgggcg ccgtcgacca gccgggggag
     1921 cggcgcgtcc acgagcggcc caccccgcgg ctgggcgggc tggccatctt catgggcctg
     1981 ctcgcggcga tcgtggtcgg cgcccggctg cccgggttcg agcgcgtctt cacgcacacc
     2041 agcgagcccg aggcgatcct gctcgccagc ctggcggtcg tggtgctcgg cgtgctggac
     2101 gacgtgcgcg gggtgtcggc gccggccaag ctcgccggcc agatcctgtc cgcgggcgtg
     2161 ctggtgctgt tcggcgtgac cctgctgttc gtgtacatcc ccggcaaccc gggcagtgtc
     2221 gtgtcgctgt ccaccgatct gggcgccatc gtcacggtgc tggccgtcgt ggcgatgatc
     2281 aacacggtga atctcgtcga tggcctggac gggctggccg ccgggatcgt cctgatcgcc
     2341 gcgacggcgc tgctgtccta cgtgcagttc tcggatccga cggcgctggg gctgccgtcc
     2401 gccgcctcgc tggtgctgac cgccgtgatc ggcgccacgc tgggcttcct ggtcttcaac
     2461 ttccacccgg cgtccatctt catgggcgac accggcgcca tgctgctggg actgctgctc
     2521 gccagcgccg gtgtctccgc gatcggcggc acggtgcagc ccagccgcgg cgcgttcgcg
     2581 gcgttctccg tgccggtcct ggtgccggtg ctggtcctgg cggtgccgtt cctggacacc
     2641 gcgtgggcca tcctccggcg gctccgcgcg ggccagccgg tgttctcgcc ggacaagcgc
     2701 cacctgcacc accggctcat cgagatcggc cacagccacc gccgtgccgt gctgatcatg
     2761 tactactggt cggcgctgct ggccttcgcc accgtgggcg tgggcctgct gccgctgcgc
     2821 gtcgtggccg ccgtcgtggg cgccggcgcg ctgctcgcgg tggccgtcgc ggtgctgccg
     2881 cggctggccg gccgtggctc ctccggctcc gggactcctt ccgtccgata gcggacacaa
     2941 gtggtcccgg aacgaccgcg cgagaggtcc gaaccgagcg gccgactttg tgttttcttt
     3001 cacatagtca cccatcgttt tcgtaaatac gcccctgacc tgcggaaatg cccgcagggc
     3061 gcgctttgac acgcgtgaga cccagtccgt agactccagg cggccatgac ccccccgatg
     3121 atccccggcg gtgtgccgcg cggtcgcacg gagcaatccg aggacagcag gca
//
LOCUS       DATMKC010000280         5168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_241340, whole genome shotgun sequence.
ACCESSION   DATMKC010000280 DATMKC010000000
VERSION     DATMKC010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241340"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VNU01_08265"
     CDS             complement(<1..293)
                     /locus_tag="VNU01_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32651.1"
                     /translation="MSRGELDPDIAAMLRETPAHAPYAEMGAAAARETFRSSVVASRP
                     AGWAPEPVAEVHDLLIPGPQTAIPVRLYHPEPGTRLPVVVYFHGGGWATGSLD"
     gene            complement(298..1263)
                     /locus_tag="VNU01_08270"
     CDS             complement(298..1263)
                     /locus_tag="VNU01_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH32652.1"
                     /translation="MADYTGVDPQVVRVLETIARVNPPRYQDVGHEQARVLSAAVAAQ
                     RAERHGVEPVKSVEDRVIAGPGGDLRLRVYWPQDAAHEDKLPVVLFVHGGGWTVGSVD
                     TYDGPARATCNRTGAVLVSVDYRLAPEHPYPAALDDGWAALRWTAEHAASLGGDPARI
                     GVWGDSAGGNICAALTLRARDAGGPPIKAQCLVYVSVDASKPYRSAEEFAEGFVLTAA
                     TSRWFSNNYLPEFAMRLEPGASPLLADSHAGLPPAVIAVGSHDVLRDHSAVYAEKLRA
                     DGVAVWHRVYDGLVHTFYELAAYVDAAREARDEICAQFRTLLLAE"
     gene            complement(1310..2740)
                     /locus_tag="VNU01_08275"
     CDS             complement(1310..2740)
                     /locus_tag="VNU01_08275"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWH32653.1"
                     /translation="MTRAHFVDGRWTPSLSDDRIAVLNPATGQTLDDIPGGSAAEADA
                     AVAAARKAAGQWARTPATERAACLKEAARLVRAQAEDLALAQTAENGKPLGMSRGDID
                     AAAGTIEQFAELGPLHRGRSLVGAWDAVDTMVHEPYGVAACIVPWNDPLGIAAQMLAA
                     ALVAGNTVVLKPSEKTPLSAVAMVELFARHDVFPDGVLDLLLGDHRAGWPLVEHPDVD
                     LVLHTGSIATGRRIAERCGALMRKAVLELGGKDPVLVDRDVDPGWAAEQVAAGAFANC
                     GQICTSAERVYVHADIAEAFTARLAEQASALRFGDPHDGATQMGPLIDARQRDLVHRH
                     VCEAVAAGARLVTGGQVPDGAGFFYPATVLADVTDDMLVMREETFGPVAPVRTVPSFD
                     EAIEAANRTEYGLAATVLTADQANAQKAWRELRAGTVKVNSAWGGAPGGAAEPHKASG
                     RGYGYGPELLDEVTVTKVVHYEPARR"
     gene            complement(2792..3166)
                     /locus_tag="VNU01_08280"
     CDS             complement(2792..3166)
                     /locus_tag="VNU01_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HWH32654.1"
                     /translation="MTEYLTVVEVMALHRLLLQRDGGTAGIRDPGALQAALYRPLTGY
                     YTDLVEEAAALFESLIVNHAFLDGNKRVAFAAMDVFLRLNGMRLTAEPTAIHAFLMDL
                     FDRNALRFEHVEPWLRRWTGPA"
     gene            complement(3168..3395)
                     /locus_tag="VNU01_08285"
     CDS             complement(3168..3395)
                     /locus_tag="VNU01_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32655.1"
                     /translation="MTERPEKFATQAPHELLAEVRDIARTEGRQFQAVVAEALAEYVT
                     RKRGSRPRPEVLAHFEASFERNRELYRRLAE"
     gene            3474..4535
                     /locus_tag="VNU01_08290"
     CDS             3474..4535
                     /locus_tag="VNU01_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32656.1"
                     /translation="MPKLVRADTEEPGITRRGRGRGFEYVDEATGQKITDPDTLQRIK
                     DLVIPPAWKDVWVCPDPHGHIQVLGTDEAGRRQYRYHEAWREQRDREKFSKMSDFARA
                     LPRLREACDALLDDPTDLRRGTVLACAARLLDHGFFRIGSECYAEQNDSYGLATMRKE
                     HVIVSGDTVVFDYAGKSGKRRVQSVVNPAVAEVVRRLKRRRGGGEELLAYKDEDAAWR
                     DVTSADINDWIKLVLGEEFSAKDFRTWNATVLAAVALAVSGEAVTGSETAKKRAVTRA
                     VQEVAHYLGNTPAVCRSSYIDPRVIERFSDGWTIAGVLDELGGEAAWGRPVIQGAVER
                     AVLDLLEERSRSEFIEKAG"
     gene            complement(4607..5002)
                     /locus_tag="VNU01_08295"
     CDS             complement(4607..5002)
                     /locus_tag="VNU01_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWH32657.1"
                     /translation="MSEPIRAAAFDLFDDPNYAVVATLMPDGSPQATVVWIERDGDRA
                     VLFNTERGRSKPANLERDPRVAVTVIDHDNPYRYVQVRGRATLTEEGARAHIDALARK
                     YTGKDYSKHREDRARVIVRVEPESIDYHG"
BASE COUNT          694 a         1851 c         1888 g          735 t
ORIGIN      
        1 ctgtcgaggc tgccggtagc ccacccgccg ccgtggaagt acacgacgac cggcaggcgc
       61 gtgcccggct ccgggtggta cagccgcacg gggatcgcgg tctgcggtcc cgggatcagc
      121 aggtcgtgga cctcggcgac cggctccggc gcccagccgg ccggtcggga ggcgaccacc
      181 gaggagcgga aggtctcccg ggcggcggcg gcgcccatct cggcgtaggg cgcatgagcg
      241 ggcgtctcgc gcagcatcgc ggcgatgtcg gggtccagct caccacggct cacgcgctca
      301 ctccgccagc agcagcgtcc ggaactgcgc gcagatctcg tcgcgcgcct cgcgtgccgc
      361 gtcgacgtac gcggcaagct cgtagaacgt gtgcaccagc ccgtcgtaga cgcggtgcca
      421 gaccgccacg ccgtcggccc gcagcttctc ggcgtagacg gcgctgtggt cgcgcaggac
      481 gtcgtggctg cccacggcga tcaccgcggg gggcaggccg gcgtggctgt cggccagcag
      541 cgggctggct cccggctcca ggcgcatggc gaactcgggc aggtagttgt tgctgaacca
      601 gcgcgaggtc gcggccgtca gcacgaagcc ctcggcgaac tcctcggcgg agcggtacgg
      661 cttgctggcg tccacgctga cgtacaccag gcactgcgcc ttgatcggcg ggccgcccgc
      721 gtcgcgcgcc cgcagcgtca gcgcggcgca gatgttgccg cccgcgctgt cgccccacac
      781 gccgatgcgc gccgggtcgc cgccgaggct cgcggcgtgc tcggccgtcc accgcagagc
      841 cgcccagccg tcgtccagcg cggccgggta gggatgctcc ggggccagcc ggtagtcgac
      901 ggagaccagg acggcgcccg tgcggttgca cgtggcgcgc gccggaccgt cgtaggtgtc
      961 gacgctgccg acggtccacc cgccgccgtg cacgaacagc accaccggaa gcttgtcctc
     1021 gtgcgcggcg tcctgcggcc agtacacgcg gaggcgcaga tcgccgccgg ggccggcgat
     1081 gacgcggtcc tccactgact tcaccggctc gacgccgtgg cgttcggcgc gctgagccgc
     1141 gacggccgcc gacagcacgc gcgcctgctc gtggccgacg tcctgatagc gggggggatt
     1201 gacgcgggcg atggtctcca aaacccgcac cacttgggga tcgacgccgg tgtagtccgc
     1261 catgacgcgc aagcctaggc gacccgaatc gggcgaaccc aatgggtctt cagcgccgcg
     1321 ccggctcgta gtggacgacc ttggtgaccg tgacctcgtc cagcagttcc gggccgtagc
     1381 cgtagccgcg tccgctggcc ttgtgcggct ccgctgcgcc gcccggcgcg ccgccccagg
     1441 cgctgttgac cttcaccgtg ccggcgcgta gttcgcgcca cgccttctgc gcgttggcct
     1501 ggtccgcggt gagcacggtc gcggccagcc cgtactcggt gcggttggct gcctcgatcg
     1561 cctcgtcgaa gctcggcacg gtgcggaccg gcgccaccgg gccgaacgtc tcctcgcgca
     1621 tcaccagcat gtcgtcggtc acgtcggcca ggacggtggc ggggtagaag aacccggcgc
     1681 cgtcggggac ctgcccgccg gtcaccaggc gcgctcccgc ggcgaccgcc tcgcagacat
     1741 ggcggtgcac cagatcgcgc tggcgcgcgt cgatgagcgg gcccatctgc gtggcgccgt
     1801 cgtgcgggtc gccgaagcgc agcgcggagg cctgctcggc caggcgggcc gtgaacgcct
     1861 cggcgatgtc ggcgtggacg tagacgcgct ccgcggacgt gcagatctgg ccgcagttgg
     1921 cgaacgcgcc cgcggccacc tgctcggccg cccagcccgg gtccacgtcg cggtcgacca
     1981 ggaccgggtc cttgccgccg agctccagca ccgccttgcg catgagcgcg ccgcagcgct
     2041 cggcgatgcg gcggccggtc gcgatggagc cggtgtgcag caccaggtcg acgtcgggat
     2101 gctccaccag cggccagccg gcccggtggt cgccgagcag gaggtccagc acgccgtccg
     2161 ggaagacgtc gtgccgggcg aacagctcca ccatggccac ggccgacagc ggcgtcttct
     2221 cgctcggctt gagcaccacc gtgttgccgg ccaccagcgc ggccgccagc atctgcgccg
     2281 cgatgcccag cgggtcgttc cacggcacga tgcaggccgc cacgccgtag ggctcgtgca
     2341 ccatggtgtc cacggcgtcc caggcgccga ccaggctgcg gccgcggtgc aacggcccca
     2401 gctcggcgaa ctgctcgatg gtccccgcgg ccgcgtcgat gtccccacgc gacatgccca
     2461 ggggcttgcc gttctccgcg gtctgcgcca gcgccaggtc ctcggcctgc gcgcggacga
     2521 gccgggccgc ctccttgagg caggccgcgc gttccgtggc gggcgtgcgc gcccactgcc
     2581 ccgccgcctt gcgggcggcg gccaccgcgg cgtcggcctc ggcggcgctg ccaccgggga
     2641 tgtcgtccag cgtctggccc gtggccgggt tcagcaccgc gatgcggtcg tcggacaggg
     2701 acggcgtcca cctgccgtcc acgaagtgcg cgcgcgtcat acgtcctcct gcgaggtggt
     2761 cgccgccacg tcttccccgc cggtgccggt cctacgccgg tcccgtccag cggcgcagcc
     2821 acggctcgac gtgctcgaag cgcagggcgt tgcggtcgaa gaggtccatg aggaacgcat
     2881 gaatggccgt cggctccgcc gtgaggcgca tcccgttgag cctgaggaac acgtccatcg
     2941 ccgcgaaggc gacgcgcttg ttcccgtcca agaacgcatg gttgacgatc aggctctcga
     3001 agagcgcggc ggcctcctcc accaggtcgg tgtaatagcc ggtgagcggc cggtacaggg
     3061 ccgcctggag cgctccggga tctcgtatcc cggcggtgcc accatcgcgt tgcagcagga
     3121 gccggtggag cgccatcacc tccacgacgg tgaggtactc ggtcatctca ttcggccagc
     3181 cggcggtaga gctcccggtt gcgctcgaag ctcgcctcga agtgggccag gacttccggc
     3241 ctcggccgtg atccccgctt gcgcgtgacg tattccgcca gcgcctcagc gaccactgcc
     3301 tggaactggc gcccttcggt gcgtgcgatg tcgcggacct cggcaagcaa ctcgtggggc
     3361 gcctgggtgg cgaacttctc tggccgctca gtcatcgtgc cagcttgaca catcaagtca
     3421 taccttgaca ggggtcgcgt gtcgcgttgt ggacgccggg ggagatcgcg gccatgccga
     3481 agctggtgcg tgccgacacc gaggagccgg ggatcacccg gcggggccgc gggcgcggct
     3541 tcgagtacgt cgacgaggcc accgggcaga agatcaccga ccccgacacg ctgcagcgca
     3601 tcaaggacct cgtgatcccg ccggcctgga aggacgtgtg ggtctgcccc gacccgcacg
     3661 ggcacatcca ggtgctgggc accgacgagg ccggtcggcg ccagtaccgc tatcacgagg
     3721 cctggcgcga gcagcgcgac cgcgagaagt tctccaagat gtcggacttc gcgcgcgcgc
     3781 tgccgcggtt gcgcgaggcc tgcgacgcgc tgctcgacga cccgaccgac ctgcgccgcg
     3841 gcaccgtgct ggcctgcgcg gcgcgcctgc tcgaccacgg cttcttccgc atcggcagcg
     3901 agtgctacgc cgagcagaac gactcctacg ggctggcgac catgcgcaag gagcacgtca
     3961 tcgtgtccgg cgacaccgtg gtgttcgact acgcgggcaa gagcggcaag cgccgggtcc
     4021 agtccgtggt gaacccggcc gtggccgaag tggtgcggcg cctgaagcgc cgccgcggcg
     4081 gcggtgagga attgctggcg tacaaggacg aggacgccgc ctggcgcgac gtcacgtcgg
     4141 ccgacatcaa cgactggatc aagctggtgc tcggcgagga gttcagcgcg aaggatttcc
     4201 ggacctggaa cgccacggtg ctggcggccg tcgccctggc cgtgtccggg gaggcggtca
     4261 cgggcagcga gacggcgaag aagcgcgcgg tgaccagggc ggtccaggag gtggcccact
     4321 acctgggcaa cacgcccgcg gtgtgccgct ccagctacat cgacccgcgg gtgatcgagc
     4381 gcttctcaga cggctggacc atcgcggggg tgctcgacga actcggcggc gaggccgcct
     4441 ggggccggcc cgtcatccag ggtgccgtgg agcgggcggt gctggacctg ctggaggagc
     4501 gctcgcggag cgaattcatc gaaaaggccg gctgaggagt gggccaggac gggaggcgcg
     4561 agcggtgctc tttctttccc gagcgccgac ccgtccgggc ccactcctag ccgtgatagt
     4621 cgatggactc gggctccacg cgcacgatca cgcgggcccg gtcctcgcgg tgcttgctgt
     4681 agtccttgcc ggtgtacttg cgcgccagcg cgtcgatgtg cgcacgcgcg ccctcctcgg
     4741 tgagggttgc gcgcccgcgg acctgcacgt agcggtacgg gttgtcgtgg tcgatcaccg
     4801 tgaccgccac gcgcgggtcc cgctccaggt tggccggctt ggacctgccg cgctcggtgt
     4861 tgaacagcac cgcgcggtcg ccgtcgcgct cgatccagac caccgtcgcc tgcggcgagc
     4921 cgtcgggcat gagggtggcg accacggcgt agttggggtc gtcgaacagg tcgaacgcgg
     4981 cggcgcggat cggctcggac atggtgtggc gcactcctgg tgtgggcgga cagcgacatg
     5041 acgctacccc ggcggcggct ggacaccccc tggctagact cggacccatg tccgattccg
     5101 ccgccccggc gacctccgtc tccccgctgg tcgacgggct caatcccgag caggcggccg
     5161 ccgtggtg
//
LOCUS       DATMKC010000281         3160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_148998, whole genome shotgun sequence.
ACCESSION   DATMKC010000281 DATMKC010000000
VERSION     DATMKC010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148998"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VNU01_08300"
     CDS             <1..1197
                     /locus_tag="VNU01_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32658.1"
                     /translation="LTACATESTDDTAATTAEAGGDRSEPATEHSEGADDTAATAQAA
                     DLRAGLNTLLAEHVFFAGAAVNEALQGNTAGFEAAAGALDENSVALSEAVGSVYGPEA
                     GEAFLGLWRSHIGMFVDYTQATAAGDKAAQDKAVADLTGYANDFAVFLSGANPNLPEE
                     TVEQLVVEHVVSTKDAVDALASGDAAKGFSEFREAIAHMHMIGDPLSAAIAQQFPEQF
                     STGTSEAASALRTQLNVKLAEHVWLAGAAVNEAAKGNTAAFEAAAGALDGNSQDLAGM
                     VGSVYGPEAGEAFLGLWRAHIGMFVDYTQGKLAGDDAKAAKAEQDLAGYSRDFAVFLN
                     GANDNLPVEDVQALLEEHVASTKGAIDAVVGGEGNPFVALREAGQHMSHIADPLAAAI
                     ATQQNL"
     gene            1305..1736
                     /locus_tag="VNU01_08305"
     CDS             1305..1736
                     /locus_tag="VNU01_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWH32659.1"
                     /translation="MGRTIMAMVAALTLLLGACGGGGDEGVADQPQTGQSQTGQSEPG
                     QGDQPATEASGQGGSDVAVQGFRFQPATLQVAAGGTVTWTNEDDINHTATAGTPDAPT
                     GEFDVDLPQKGATGEHTFDEPGTYAYFCEVHPSMTGEIVVS"
     gene            1810..2424
                     /gene="sigK"
                     /locus_tag="VNU01_08310"
     CDS             1810..2424
                     /gene="sigK"
                     /locus_tag="VNU01_08310"
                     /inference="COORDINATES: protein motif:HMM:NF007228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HWH32660.1"
                     /translation="MGQVVRRLRAERAGLRALPGGAGTADADALLRRAGRGDRDAYAK
                     LYDAVAGQVYGLVRRVLRDPAQSEEVAQEVLLEVWRLAPRFDPERGSARAWIMTLAHR
                     RAVDRVRSEQASRDREDKAGRASVERERDEVVEEVEARMERQRVRQALTQLTDLQREA
                     LELAYYGGHTYREVADILQAPLGTVKTRLRDGLIRMRDELGVGE"
     gene            2421..>3160
                     /locus_tag="VNU01_08315"
     CDS             2421..>3160
                     /locus_tag="VNU01_08315"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWH32661.1"
                     /translation="MTADIHTLAGAYALHALSDEEERLFLRHLAVCDSCQTEVAEFER
                     TAALLGGAVEEPPPAGMRAAVLAAVDRTPQVRDGLRPPGWRERIAPVLMPVAAGLGLV
                     VMALGGVAFQQARELRDLRATQTATSEALLAVLGAPDLERIAMTGDGTATVLWSPAGR
                     QAVLVAEGLAPPPQGKAYQLWLLRDGVPVPSQVFRPGPDGAVVAYLATVPDAFQAAGD
                     VSTDSHARQLTGPARPRSRGGQHHRPARG"
BASE COUNT          460 a         1140 c         1210 g          350 t
ORIGIN      
        1 ctgaccgcct gcgccaccga gagcaccgac gacaccgccg cgaccaccgc cgaagccggc
       61 ggggaccggt ccgagcccgc gacggagcac agcgagggcg cggacgacac cgccgccacc
      121 gcgcaggccg ccgacctccg cgccgggctg aacacgctgc tcgccgagca tgttttcttc
      181 gcgggcgccg ccgtgaacga ggcgctgcag ggcaacaccg ccggcttcga ggccgccgcg
      241 ggcgcgctcg acgagaactc cgtcgcgctg tcggaggccg tcgggtccgt ctacggcccc
      301 gaggccggtg aggcgttcct cggcctgtgg cgcagccaca tcggcatgtt cgtcgactac
      361 acgcaggcca cggccgccgg cgacaaggcc gcgcaggaca aggctgtcgc ggacctgacc
      421 gggtacgcca acgacttcgc ggtcttcctg agcggcgcga acccgaacct gccggaggag
      481 accgtcgagc aactggtcgt cgagcacgtg gtgtccacca aggacgcggt cgacgcgctc
      541 gcctcgggtg acgcggccaa gggcttcagc gagtttcgcg aagccattgc gcacatgcac
      601 atgatcggcg acccgctgtc ggccgccatc gcccagcagt tccccgagca gttctccacc
      661 ggcaccagcg aggccgcctc cgcgctgcgc acgcagttga acgtcaagct ggccgagcac
      721 gtctggctgg ccggcgcggc agtcaacgag gcggccaagg gcaacaccgc ggcgttcgag
      781 gccgccgcgg gcgcgctcga cggcaactcc caggacctcg ccggcatggt cggctcggtg
      841 tacggcccgg aggccggcga ggcgttcctg ggcctgtggc gcgcgcacat cggcatgttc
      901 gtcgactaca cgcaaggcaa gctcgccggc gacgacgcca aggccgccaa ggcggagcag
      961 gacctcgccg gctacagccg cgacttcgcg gtgttcctca acggcgcgaa cgacaacctg
     1021 ccggtcgagg acgtgcaggc cctgctggag gagcacgtcg ccagcaccaa gggcgcgatc
     1081 gacgccgtgg tgggcggcga gggcaacccg ttcgtggcgc tgcgcgaggc cggccagcac
     1141 atgagccaca tcgccgaccc gctggcggcg gccatcgcca cccagcagaa cctgtaagga
     1201 tcgcctggcc gactgcggcg cgccctcccc cggagggcgc gccgcggggt ggcgacaggg
     1261 tcccgagggc atgacacacc cgggacacga cttcggaggg cgacatggga cgcacgatca
     1321 tggcgatggt ggccgcgctc acgctgctgc tcggcgcgtg cggcggcggc ggggacgagg
     1381 gcgtggccga ccagccccag accggtcaga gccagacggg ccagagcgaa ccgggccagg
     1441 gggaccagcc ggcgactgag gcatccggcc agggcggcag cgacgtggcg gtgcaggggt
     1501 tccggttcca gccggcgacc ctgcaggtcg cggccggcgg caccgtgacg tggacgaacg
     1561 aggacgacat caaccacacc gccacggccg ggacaccgga cgcgcccacg ggcgagttcg
     1621 acgtcgacct gccgcagaag ggcgcgaccg gcgagcacac gttcgacgag ccgggcacgt
     1681 acgcctactt ctgtgaggtg cacccctcga tgacgggcga gatcgtcgtc tcctagaccg
     1741 gcaccggacc aatccggggc gggcggggtg acgaaccacc gtcgtcccgc cgacacagaa
     1801 ggagcaccct tgggccaggt cgtccgccgc ctccgcgccg agcgcgcggg cttgcgcgcc
     1861 ctgcccggcg gcgccggcac cgccgacgcc gacgcgctcc tgcgcagagc cggccggggc
     1921 gaccgcgacg cgtacgcgaa gctgtacgac gcggtcgccg gccaggtcta cgggctggtc
     1981 cggcgcgtgc tgcgcgaccc ggcccagtcg gaggaggtcg cgcaggaggt gctgctggag
     2041 gtctggaggc tcgcgccgcg cttcgacccg gagcgcggaa gcgcccgggc gtggatcatg
     2101 acgctggcgc accgccgggc ggtggaccgg gtccgcagcg agcaggcgag ccgggaccgg
     2161 gaggacaagg ccggccgggc gtccgtggaa cgggaacgcg acgaggtcgt cgaggaggtg
     2221 gaggccagga tggagcggca gcgggtgcgc caggcgctga cccagctcac cgacctccag
     2281 cgcgaggccc tcgagctcgc ctactacggc gggcacacct accgggaggt cgccgacatc
     2341 ctccaggcgc cgctgggcac cgtgaagacc cggctgcgcg acggcctgat ccggatgcgc
     2401 gacgagctgg gggtgggcga atgaccgcgg acatccacac gctggccggc gcctacgcgc
     2461 tgcacgcgct cagcgacgag gaggagcggc tgttcctgcg gcacctggcc gtgtgcgact
     2521 cctgccagac cgaggtcgcc gagttcgagc gcaccgcggc gctgctgggc ggggcggtcg
     2581 aggagccgcc gccggcgggc atgcgcgcgg cggtgctggc ggcggtggac cgtaccccgc
     2641 aggtgcgcga cggcctgcgc ccgccggggt ggcgggagcg catcgccccg gtgctcatgc
     2701 cggtggccgc ggggctcggc ctggtcgtca tggcgctggg cggcgtggcg ttccagcagg
     2761 ctcgtgagct gcgggacctg cgcgcgacgc agaccgccac gtcggaggcg ctgctcgcgg
     2821 tgctcggcgc cccggacctg gagcgcatcg ccatgaccgg cgacggcacc gccacggtgc
     2881 tgtggtcgcc ggccggccgg caagccgtgc tggtcgccga ggggctcgcc ccgccgccgc
     2941 aaggcaaggc ctaccagctg tggctgctgc gcgacggggt gccggtgccg tcccaggtct
     3001 tccggccggg ccccgacggc gccgtggtgg cgtacctggc gacggtgccg gacgcgttcc
     3061 aggccgccgg ggacgtgagc accgattcgc atgcgcgcca gcttacgggt ccggcacggc
     3121 cgcgctcacg cgggggtcag caccatcggc ccgctcgggg
//
LOCUS       DATMKC010000282         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_21676, whole genome shotgun sequence.
ACCESSION   DATMKC010000282 DATMKC010000000
VERSION     DATMKC010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21676"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VNU01_08320"
     CDS             <1..549
                     /locus_tag="VNU01_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32662.1"
                     /translation="DAAAGTRVRHLSGGQKQRLSLALALIGRPRVAILDEPTVGMDPQ
                     ARRATWELIEELRADGVTVLLTTHVMDEAERLADLVAVIDRGRLLALDTPAALLHAHR
                     PGRLTVHTTAALDPAAFAAGVGADVRREGPERWSVDAGPEAIARVSAWFADHGLPLTG
                     VTAAGGGLEEVFLKLTDEAGGR"
     gene            546..1292
                     /locus_tag="VNU01_08325"
     CDS             546..1292
                     /locus_tag="VNU01_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH32663.1"
                     /translation="MTAAPSLRRLRAQSLMELRLLLRNGENLLVTMGIPTGLLVFFSL
                     VDVLPTDGRPAVEFLAPGVLALAVMGAALVSLGISTGFERHYLVLKRLGATPLTRGEL
                     VAAKALSVLAVELVQVVVVLGVAAALGWRPEAGAAGVAAAVGAVLAGTAAFAGIGLAL
                     AGTLRAVGTLALVNALFLLLMLLGGMVFSLDVLPEGLAAAARLLPSAALADALRSALT
                     QAAVDGRALGVLAAWATLSVTAAARLFRWE"
     gene            complement(1331..1867)
                     /locus_tag="VNU01_08330"
     CDS             complement(1331..1867)
                     /locus_tag="VNU01_08330"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWH32664.1"
                     /translation="MGKVLEALDERLTSWIAAQRLFFVATAPRAGDHVNLSPKGGDTF
                     RVLSPTEVAYLDLTGSGAETIAHLRENGRITVMFCAFSGPPDIVRLFGRGEAHPVGSP
                     GYDEIAHLFPEKLGARAIVRVALTRVQTSCGYQVPLYDYMGERDQLDRWAESKGRAGI
                     LDYQAQRNAVSIDGLPAL"
     gene            complement(1868..2788)
                     /locus_tag="VNU01_08335"
     CDS             complement(1868..2788)
                     /locus_tag="VNU01_08335"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWH32665.1"
                     /translation="MSDAVAPVPAGASGRASAGERVRAYVGLTKPRIIELLLVTTVPT
                     MVVAERGIPDLWLVLATLIGGFLAAGSANTFNSWFDADIDQIMHRTSRRPLPRHQVPP
                     RNALRFGVVLGVASFVWLSVMVNLLSAVLGVVAILFYVLVYTMLLKRRTTQNIVIGGA
                     AGCMPVLIGWAAVTGEVGLPAWVLFAIIYYWTPPHFWALAIKYSDDYARAGVPMLPVV
                     RGVVDTTRQILLYTLLLVAVTLMFGPIGGMGVIYLAAALLLGGVFIGLAWQLYRRKTE
                     ELAMRVFRYSISYLGLLFVAMAADAVVYAG"
BASE COUNT          378 a         1024 c         1067 g          377 t
ORIGIN      
        1 gacgcggcgg cgggcacgcg cgtgcggcac ctgtccggcg gccagaagca gcggctcagc
       61 ctggcgctcg cgctcatcgg caggccgcgc gtggcgatcc tggacgagcc cacggtcggc
      121 atggacccgc aggctcgccg cgccacgtgg gagctcatcg aggagctgcg cgccgacggg
      181 gtcacggtcc tgctgaccac ccacgtcatg gacgaggccg agcgcctcgc cgacctcgtg
      241 gccgtgatcg accgcggccg tctgctcgcg ctggacacgc cggccgcgct gctgcacgcc
      301 catcgacccg gccggctcac cgtccacacc accgcggcgc tcgacccggc ggcgttcgcg
      361 gccggcgtgg gcgcggacgt gcggcgcgag ggcccggagc gctggtcggt ggacgcgggg
      421 cccgaggcca tcgcgcgcgt gagcgcgtgg ttcgccgacc acgggttgcc gctgaccggg
      481 gtgaccgccg ccggcggagg cctggaagag gtctttttga agctgaccga cgaggcgggc
      541 ggccggtgac cgccgcgccg agcctccggc ggctgcgcgc gcagtccctc atggaactgc
      601 ggctgctgct gcgcaacggc gagaacctgc tcgtgacgat gggcatcccc acgggcctgc
      661 tggtgttctt ctcgctggtc gacgtgctgc ccacggacgg ccgccccgcc gtggagttcc
      721 tggcgccggg cgtgctggcg ctcgcggtga tgggtgcggc gctggtctcg ctcggcatct
      781 ccaccggttt cgagcgccac tacctggtgc tcaagcgcct gggggcgacg ccgctcacgc
      841 gcggggagct cgtggccgcc aaggcgctgt cggtcctggc ggtggagctg gtccaggtcg
      901 tggtggtgct cggtgtcgcc gccgcgctgg gctggcggcc ggaggcaggc gccgcggggg
      961 tcgcggccgc cgtgggcgcc gtcctggcgg ggaccgcggc gttcgccggc atcggcctgg
     1021 cgctggcggg cacgctgcgc gccgtcggca ccctggcgct ggtcaacgcg ctgttcctgc
     1081 tgctcatgct gctcggcggc atggtgttct ccctggacgt gctgcccgag ggcctcgcgg
     1141 cggcggcgcg gctgctgcct tcggccgcgc tggccgacgc gctgcgctcc gccctcacgc
     1201 aagccgccgt ggacggccgg gcactgggcg tgctggccgc ctgggccacc ctgagcgtca
     1261 ccgcggccgc gcgcctgttc cgctgggagt agcaacaggc gcaggacgtg cccttcgcca
     1321 cccccgggtg ctacagagcg ggcagcccgt cgatgctgac ggcgttgcgc tgcgcctggt
     1381 agtcgagaat gcccgcccgc cccttcgact ccgcccagcg gtcgagctgg tcgcgctccc
     1441 ccatgtagtc gtacagcggc acctggtagc cgcagctcgt ctgcacgcgc gtgagcgcca
     1501 cgcggacgat cgcgcgggcg cccagtttct cggggaacag gtgggcgatc tcgtcgtagc
     1561 cgggtgatcc cacgggatgg gcctcgccgc ggccgaacag gcgcacgatg tccggcgggc
     1621 cggagaaggc gcagaacatc accgtgatgc gcccgttctc gcgcaggtgc gcgatggtct
     1681 ccgcgccgct gccggtgagg tccaggtagg cgacctcggt cggcgagagc acacggaagg
     1741 tgtcgccgcc cttgggcgag aggttgacgt gatcccccgc gcgcggcgcg gtggccacga
     1801 agaagagcct ctgcgcggcg atccaggagg tcaggcgctc gtcgagcgcc tccagcacct
     1861 tgcccactca gccagcgtag accacggcgt cggccgccat ggccacgaac agcaggccca
     1921 ggtagctgat cgagtagcgg aacacccgca tcgccagctc ctcggtcttg cgccggtaca
     1981 gctgccacgc caggccgatg aacaccccgc cgagcagcag ggcggcggcc aggtagatga
     2041 cgcccatgcc gccgatcggc ccgaacatca gcgtcacggc caccagcagc agcgtgtaca
     2101 gcaggatctg gcgcgtggtg tccacgacgc cccgcaccac cggcagcatc ggcacgccgg
     2161 cgcgggcgta gtcgtcggag tacttgatcg ccagcgccca gaagtgcggc ggtgtccagt
     2221 agtagatgat cgcgaacagg acccacgcgg gcaggccgac ctcgccggtg acggcggccc
     2281 agccgatcag cacgggcatg cagccggcgg cgccgccgat cacgatgttc tgggtggtgc
     2341 ggcgcttcag cagcatcgtg tacacgagca cgtagaacag gatcgccacg acgccgagca
     2401 cggccgacag caggttcacc atcaccgaca gccacacgaa gctggccacg cccaggacga
     2461 cgccgaagcg cagcgcgttg cggggcggca cctggtggcg cggcagcggc cggcgggacg
     2521 tgcggtgcat gatctggtcg atgtcggcgt cgaaccagct gttgaacgtg ttggcgctgc
     2581 cggccgccag gaagccgccg atcagcgtcg cgagcaccag ccacaggtcg gggatcccgc
     2641 gctcggccac gaccatggtg ggcaccgtgg tgaccagcag cagctcgatg atgcgcggct
     2701 tggtcaggcc cacgtaggcg cgcacgcgct cgccggccga cgcgcggccg gaagccccgg
     2761 cgggcacggg ggcgacggcg tcgctcatcg cgcggcctcc gcgcgccgcg cgagcagcgt
     2821 gccgagcacg agcacggccc agatcc
//
LOCUS       DATMKC010000283        12789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_82516, whole genome shotgun sequence.
ACCESSION   DATMKC010000283 DATMKC010000000
VERSION     DATMKC010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12789
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82516"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1279
                     /locus_tag="VNU01_08340"
     CDS             44..1279
                     /locus_tag="VNU01_08340"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH32666.1"
                     /translation="MVADAKLAIMDEFDRGAGPLVARMMEGFRASIPAYGALPEETLR
                     QVREVVSENLACFSRCLRRGSDPTPEELAPFRDSASRRAREGVSLAGVLEAYRMGSRV
                     AWRELREIVEAHGEFALGLEFATRVMSYIDSVSESVAEAYISEFEELASDREAARRDF
                     IDGLLDGSLADAELLARAEALGLDVASPYVVCLICMVEGSAEGAELRAKQRTLRAMAA
                     SLPHGHRSLIVNRGHELVAVVPSRQGAEDELSERLGRFVSRAAEVLGERLAAGIGRVH
                     DDVTELASGYREATVALAAAKGGEAGIAVYGQVLMEELLLREPAIARRMVQATLQPLQ
                     AHPYLLETLETYLRHGPSLPDVAERLHVHANTVAYRLGRVRDLTGRDPRTPNGIAHLA
                     LALRAAELLGGLPEQPTRP"
     gene            1351..2178
                     /locus_tag="VNU01_08345"
     CDS             1351..2178
                     /locus_tag="VNU01_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HWH32667.1"
                     /translation="MTLAMTLVMAMPLAAAPGGGNGGGGGGSTTLPNSIASLGDSITQ
                     AANLDGGNIGASHPEHSWSTGYDSGDIVNSHYERLLANNRKIKGQTFNKAVSGAKMDD
                     APAQADNVVATGAQYVTFLMGGNDVCTSSNTNMTPLSSFETNFRLAMDKLTTGLPDAK
                     IYVLSVPDVYQLWRLFDGEWYPEWVWSTFDICQSMLDNSRTEADRQAVRAHNMAFNDI
                     LAAVCAEYTQCLWDNYGVFNYQFTRADVSGVDYFHPSMTGQKNLAQQSWAGGYWPTL"
     gene            2269..3846
                     /locus_tag="VNU01_08350"
     CDS             2269..3846
                     /locus_tag="VNU01_08350"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HWH32668.1"
                     /translation="MAAEYDYIVVGAGSAGCVLAARLTEDPDVRVLLLEAGGPADHPN
                     IAVPAAGPALWRTSVDWNFTTMPQKHLRERALYWPRGKVLGGSSSINAMIYIRGNRAD
                     YDEWAALGNAGWGYDDVLPYFKRAEDNARGADEFHGAGGPLRVEDPRWVSTLGSAFVE
                     AAQAAGLPANPDFNGATQDGVGVYQVTQRRGERWSTFKAYLEPALARPNLEVRTHALT
                     HRVLVEAGRATGVEYSHAGEVRIARAQGEVLLCGGAVASPQLLQLSGIGPADHLREVG
                     VAVVADLPGVGENLQDHLVADWSHRCEADSLFEADRSTGNILAYLARRKGPLTSVVAE
                     VGGFVRTLDGLDAPDIQFHFLPALVDELGARTPQGHGWTMWPTLVKPLSAGTVRLRST
                     DPRWAPAIDPNYLEAPEDVDTLVRGLELSREIARQAPMARHLGERFHPPAGADDDLAE
                     YVRHNCQTLFHPVGTCRMGADELAVVDAELRVHGVDGLRVVDASVMPTVPRGNTNAPT
                     IMVAERAADLIRARVPA"
     gene            complement(3904..4404)
                     /gene="purE"
                     /locus_tag="VNU01_08355"
     CDS             complement(3904..4404)
                     /gene="purE"
                     /locus_tag="VNU01_08355"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWH32669.1"
                     /translation="MTADPLVGVVMGSDSDLSVMRGAVDVLADFGVPHEVRVVSAHRR
                     PHEMLDYGRTAAERGLKVIVAGAGGAAHLPGMLASATTLPVVGVPVPLAHMDGMDSLL
                     SIVQMPKGVPVATVGVGNGVNAGLLALRILAAADDDLAEALRQYAEDIAAASSAADAR
                     VTAEFS"
     gene            complement(4401..5540)
                     /locus_tag="VNU01_08360"
     CDS             complement(4401..5540)
                     /locus_tag="VNU01_08360"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004680.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWH32670.1"
                     /translation="MAPVVGMVGAGQLARMTAQAAVALDVRLRVLASDPADSATAVAA
                     EVVLGAPDDPDGLAALAKGADAVTFDHELVEPALIQALERDGALVRPGSGVIAVVIDK
                     RRQREALAGLGAPQPEHRVVGSREEMAAFGEAHGWPLVLKAARGGYDGRGVWVAAGID
                     EAAAVLDEAHGRGLVLLAERHVAMDCEVAVVVARRPSGEAVAYPVVQTVQRDGMLREL
                     RYPAPVSHALAGEARRLALDVAERLGVVGLLAVELFVEDGALLVNELAARPHNSGHAS
                     IEGCVTSQFANHLRAVLDWPLGATDAVAPAFALVNVVGPADGSDPAGRLPAALAVPGL
                     AALPYRCACKPGRKLGHVTVVGDDLDEVTARARRGAAILHGEETA"
     gene            5599..5763
                     /locus_tag="VNU01_08365"
     CDS             5599..5763
                     /locus_tag="VNU01_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32671.1"
                     /translation="MRAEPEITCVDCGGTCHLLNADPLEDLPEGWIVAYRCEDCLDRW
                     DLVLEEPEQR"
     gene            complement(5806..8262)
                     /locus_tag="VNU01_08370"
     CDS             complement(5806..8262)
                     /locus_tag="VNU01_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32672.1"
                     /translation="MRHTETFGTRTRGLAGALAALLAALLLVAVEPAAASHYTNTTEL
                     SGTTNTAVAASWSKATFPTSASEVLLGRDDLFADSLASGGMQGSRPLLLTSSQTLSSE
                     ADAELNRLKPSVVNIMGGPSAVSTAVEDDLKSEGYTVRRFSGPTRIETAIDIARRALP
                     AATSAIVAAAFEPPNTDPTRAFADSIAAGGWAADRNMPVLLTESQRLSTSTKNYLRGA
                     QIRTVFVVGGSSAINDAVITELQQIGMTVERVSGPTRFDTAVEIAKERGYSDASKASA
                     TILTEGQASDAWAAGFTAAAYSAANDAPIVLANGSSVPAPTGTWLAPSGKAAATELTC
                     APKVNRDACDTASEAMGHTPITGSTPRLLAATLGTTAAGATEVSFTFTEPLAAQVSAD
                     KFRLYTFDNLQVPATTAARDTARQDVVVATFPEALADDATVAAVAFEAGRGLTSNVPN
                     AEGSVGLQSVTPASGDSRGPILVSVAEEDDNEVNFTFDKPVAACGPNNKYRLVLQKQA
                     TGDTGVEVIRGTSGDAVKVDDLTCQVQLASSWHDDDNMTTDTWSGNVGRGVVDAAAVT
                     AVAGGYANPPGGAHVGATEGPDLTGVARESGSTVQMRFTFDQQIDTAVNVNQAASRFK
                     VYDSRGRVFSGTGAAPDATQGTTITRVLVTFDAGALRNVIVGGFVEDEAVKGSLLESG
                     LFNGADEEPMAPGSTTTGNTAAPRLTGITRSTSGSGSTASVTIRFTYSGTVDRGPGTY
                     HVYTDAGERVQLAGCSVVATAGNDDVDCTVSASSAVAYNAAIGAEVAGATYDAIRPAG
                     QSGFTQADSSPSPEHGGTIS"
     gene            complement(8802..9725)
                     /gene="pyrF"
                     /locus_tag="VNU01_08375"
     CDS             complement(8802..9725)
                     /gene="pyrF"
                     /locus_tag="VNU01_08375"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWH32673.1"
                     /translation="MTENFADRLLDAVDGCGTPAVVGLDPRLESMPPFLLDAARRAAQ
                     PVREAIRAFHEIVLDEAAGRVAAVKPQIAFFEQYGIEGLQAFADTLDMARERGLLVIA
                     DAKRNDIDSTAAAYANAFLGAADVFGARRPAFAADALTVTPYLGRDTLRPFVDACAEH
                     GKGIFVLVKTSNPGSGDLQDLRTDDGRTIAERVADMVAEAGEALTGARGYSAVGAVVG
                     ATYPAEARALRDRMPRAIVLVPGYGAQGGGAADAVAAFDAEGRGALVNAARTITYPKG
                     EPAGDEASLRAGLARRIEAMAADLAGALAAR"
     gene            complement(9756..11600)
                     /locus_tag="VNU01_08380"
     CDS             complement(9756..11600)
                     /locus_tag="VNU01_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH32674.1"
                     /translation="MKHTVGALCAVFSLAFSLVAPAAAAADRTVDVIVVLDARHAPGG
                     HDANKARAAQVAREHGVRATQAYGTALFGFAAAVPEGRLRALAADPRVRHVERDGAVR
                     AFGEVAPWGVARIGLDATDRSATNTGAGIHVYVVDTGIDADHADLAANLGNGYAVTSC
                     GGGGCRAAWDDDHGHGTHVAGTVGAADNDIDVVGVAPGVTLHAVKVLDKNGSGRRSGV
                     IAGVDWAAKEIAARGQAGVVNMSLGGTGSKTGTCTASGFTGSDAYHESICNATHGGAV
                     FAVAAGNSDADASGAVPAAYDDTVVTVSATQEGDDWPYWSNWGNDAASWASAASAPVA
                     VAAPGRSVVSTRRGGGTTSMSGTSMAAPHVAGALALYLAANPQPADAGAFAAARGAML
                     TEADATSSWSNSSGNPHAEPFLDARPWAPATAGPVLDPAPLVAITSPAEGATVSGAVP
                     VTADAADTEGVAKVEFFANGATTPFATDTDGTDGWSASWDSTAVADGAHTLTARATEQ
                     GAAPQTASASVVVTVDNPDTEPAPEPAGISLSATGYKIKGVKHADLAWTGATSTDVDI
                     HLDGAKAATTANDGAATVNLKSKGGGTHRFQVCEAGTGTCSPEVSVTY"
     gene            complement(11754..>12789)
                     /locus_tag="VNU01_08385"
     CDS             complement(11754..>12789)
                     /locus_tag="VNU01_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32675.1"
                     /translation="AAAVAIQVARLAAEFPGEPPAWFVRNLGLFVLPFLAAFFARRRR
                     LGARGWLGSAAPFALAALVVNLSPFRDGSATHVLVALHLPVLLWFAVACPYMGGTLRS
                     HERRMDFVRFTGEWLIYYALIALGGGVLMGLTVAILQPLGPELAERVIEWVLPSGAAG
                     AVIVAAWLVEAKQQVVENMAPVLTMLFTPLFAAMLAVAAVAYAVSGAAGAFDRELLAV
                     FDALLVVVLGLVLYAMSARDPSRPPGLMDRVQLVAVAGALVLDGMVLGAMVARIGDLG
                     LTPNRVAALGLNLLLLVNLAGTAWLSARFLTGRAAFHRLERWQTSYLPVFALWVAALV
                     AVLPPIFRFE"
BASE COUNT         1731 a         4678 c         4670 g         1710 t
ORIGIN      
        1 aaggtgttcc cgggccactc ctccgagggc taggcgcggc gccatggtcg ccgacgcgaa
       61 gctggcgatc atggacgagt tcgaccgtgg cgccggtccg ctcgtcgccc gcatgatgga
      121 gggcttccgg gcctccatcc cggcctacgg cgcgctgccg gaggagacgc tgcggcaggt
      181 ccgcgaagtc gtgtcggaga acctcgcatg cttctcgcgc tgcctgcgcc ggggcagcga
      241 ccccacgcca gaggagctgg cgccgttccg ggacagcgcg tcgcggcgcg cgcgcgaagg
      301 cgtgtcgctg gcgggcgtcc tggaggccta ccgcatgggc tcccgggtgg cctggcgcga
      361 gctgcgcgag atcgtggagg cgcacggcga gttcgcgctc ggcctggagt tcgccacgcg
      421 ggtgatgtcc tacatcgaca gcgtcagcga gtcggtggcc gaggcctaca tcagcgagtt
      481 cgaggagctg gccagcgacc gcgaggcggc gcggcgcgac ttcatcgacg gcctgctcga
      541 cggctcgctc gccgacgcgg agctgctggc gcgcgcggag gcgctcggcc tggacgtcgc
      601 gtcaccgtat gtcgtgtgcc tgatctgcat ggtggagggc tccgccgagg gcgcggagct
      661 gcgcgccaag cagcgcaccc tgcgcgccat ggccgcctcg ctgccccacg ggcaccggtc
      721 actgatcgtc aaccgcggcc acgagctcgt cgccgtggtg ccatcccggc agggcgccga
      781 ggacgagctc agcgagcggc tcgggcgctt cgtgtcccgc gccgcggagg tgctgggcga
      841 gcggctggcc gccggcatcg ggcgggtcca cgacgacgtc accgagctgg ccagcggcta
      901 ccgggaggcg accgtcgccc tggccgccgc caagggcggc gaggcgggca tcgccgtcta
      961 cgggcaggtg ctgatggagg agctgctgct gcgcgagccc gccatcgccc ggcgcatggt
     1021 gcaggccacc ctgcagccgc tgcaggcgca cccctacctg ctggagaccc tggagaccta
     1081 cctcaggcac gggccgtcgc tgcccgacgt ggccgagcgc ctgcacgtcc acgcgaacac
     1141 ggtggcctac cggctgggac gcgtacgtga tctgacgggt cgtgatccgc gtacgccgaa
     1201 cggcatcgcg cacctggcgc tcgccttgcg cgccgccgag ctgctcggcg gccttcccga
     1261 gcagcccacc aggccctagc gtcgcggcgt cactgacgtt tctttcccag ggggtgggac
     1321 atgcaccatc ggatccgatc cacactgctc atgacactgg ccatgaccct cgtcatggcg
     1381 atgccactgg ccgccgcgcc gggcggcggg aacgggggcg ggggcggcgg ctccacgacg
     1441 ctgccgaact cgatcgcctc gctgggtgac tccatcaccc aggccgccaa cctcgacggc
     1501 ggcaacatcg gcgcctcgca cccggagcac tcctggtcca ccggctacga ctccggcgac
     1561 atcgtcaaca gccactacga gcgcctgctc gcgaacaacc gcaagatcaa agggcagacg
     1621 ttcaacaagg cggtgtccgg cgcgaagatg gacgacgcgc ccgcccaggc cgacaacgtc
     1681 gtcgccaccg gcgcgcaata cgtgacgttc ctcatggggg gcaacgacgt ctgcacgtcc
     1741 agcaacacca acatgacgcc gctgtccagc ttcgagacga atttccgcct ggcgatggac
     1801 aagctgacca ccggcctgcc cgacgcgaag atctacgtgc tgagcgtgcc ggacgtgtac
     1861 cagttgtggc ggctgttcga cggcgagtgg tacccggagt gggtgtggag cacgttcgac
     1921 atctgccagt cgatgctgga caacagccgc acggaggccg accggcaggc ggtgcgcgcc
     1981 cacaacatgg cgttcaacga catcctcgcc gccgtgtgcg ccgagtacac gcagtgcctg
     2041 tgggacaact acggggtgtt caactaccag ttcacgcgcg cggacgtcag cggcgtggac
     2101 tacttccacc cgtccatgac gggacagaag aacctggcgc agcagagctg ggcaggcggc
     2161 tactggccca cgctctagcc gacacgccac gcgccctgac cctgttccgc gccgctcgcc
     2221 gccgataagg tgagcggcgc ggggcaggca cgggcgacgg gaggcggcgt ggcagcggag
     2281 tacgactaca tcgtcgtggg cgcggggtcg gcgggatgcg tgctggcggc gcgcctcacg
     2341 gaggacccgg acgtgcgggt cctgctgctg gaggccggcg ggccggccga ccaccccaac
     2401 atcgcggtgc ccgcggccgg gccggcgctg tggcgcacgt cggtggactg gaacttcacc
     2461 acgatgccgc agaagcacct gcgcgagcgc gcgctgtact ggccgcgcgg caaggtgctg
     2521 ggcggctcca gctccatcaa cgcgatgatc tacatccgcg gcaaccgcgc cgactacgac
     2581 gagtgggccg ccctgggcaa cgccggctgg ggctacgacg acgtgctgcc gtacttcaag
     2641 cgggcggagg acaacgcccg cggcgccgac gagttccacg gcgccggcgg gcccctgcgc
     2701 gtggaggacc cccgctgggt cagcacgctg ggaagcgcgt tcgtcgaggc cgcacaggcg
     2761 gccgggctgc ccgccaaccc cgacttcaac ggcgcgaccc aggacggcgt cggtgtgtac
     2821 caggtgaccc agaggcgcgg ggagcgctgg agcaccttca aggcgtacct ggagccggcc
     2881 ctggcgcggc ccaacctgga ggtgcgcacg cacgcgctga cccaccgggt gctggtggag
     2941 gcgggacgcg ccaccggcgt ggagtacagc cacgccggcg aggtgcgcat cgcgcgcgcg
     3001 cagggtgagg tgctgctgtg cggcggcgcc gtggcctccc cgcaactcct gcaactgtcc
     3061 ggcatcggcc cggccgacca cctgcgcgag gtgggtgtcg ccgtggtggc ggaccttccc
     3121 ggcgtgggcg agaacctcca ggaccacctg gtcgcggact ggtcgcaccg ctgcgaggcg
     3181 gacagcctgt tcgaagccga ccgcagcacc ggcaacatcc tggcctacct cgcgcgccgc
     3241 aaggggccgc tgaccagcgt cgtggcggag gtgggcggct tcgtgcgcac cctggacggc
     3301 ctggacgcgc ccgacatcca gttccacttc ctgcccgcgc tggtggacga gctgggcgcc
     3361 aggacgccgc aggggcacgg ctggaccatg tggccgacgc tggtcaagcc gctgtccgcg
     3421 gggaccgtgc gcctgcgctc cacggacccg cgctgggcgc cggccatcga cccgaactac
     3481 ctggaggcgc ccgaggacgt cgacacgctg gtgcggggcc tggagctgtc gcgcgagatc
     3541 gcgcggcagg cgccgatggc ccgccacctc ggcgagcgtt tccacccgcc ggcgggcgcc
     3601 gacgacgacc tcgccgagta cgtgcggcac aactgccaga cgctgttcca tcccgtgggc
     3661 acgtgccgca tgggcgccga cgagctggcg gtggtggacg cggagctgcg cgtgcacggc
     3721 gtggacggcc tgcgcgtggt cgacgcctcg gtgatgccga cggtgccgcg cggcaacacc
     3781 aacgcgccga cgatcatggt cgccgagcgc gccgcggacc tcatccgcgc ccgcgtcccc
     3841 gcctgacctg gggcttgcgg agtcgagtcc ggccgatagc ggccacgatc gcctccgcgg
     3901 cggtcaggag aactcggcgg tgacgcgggc gtcggcggcg ctgctcgctg cggcgatgtc
     3961 ctcggcgtac tgccgcagcg cctcggcgag gtcgtcgtcg gccgcggcga ggatccgcag
     4021 cgccagcagc cccgcgttga cgccgttgcc cacgccgacg gtcgcgaccg gaaccccctt
     4081 cggcatctgc acgatcgaca gcagcgagtc catgccgtcc atgtgggcca gcgggacggg
     4141 gacgccgacg accggcagcg tcgtcgcgga ggcgagcatg ccgggcaggt gggccgctcc
     4201 cccggccccg gccacgatca ccttcaggcc ccgttcggcg gcggtgcgcc cgtagtcgag
     4261 catctcgtgc ggccgccggt gcgccgagac cacgcgcacc tcgtgcggca cgccgaagtc
     4321 ggccagcacg tccaccgcgc cgcgcatcac cgacaggtct gagtcgctgc ccatcacgac
     4381 gcccaccagc gggtccgcgg tcatgccgtc tcctctccgt gcaggatcgc ggcgccgcgc
     4441 cgcgcgcgcg ccgtcacctc gtccagatcg tcgccgacca cggtgacgtg gccgagcttg
     4501 cgccccggct tgcacgcgca cctgtacggc aaggccgcca agccgggcac ggccagtgcc
     4561 gcaggcaggc gcccggccgg gtcggacccg tccgccggcc cgaccacgtt gaccagcgcg
     4621 aacgccggcg cgaccgcgtc ggtggcaccg agcggccagt cgagcaccgc gcgcagatgg
     4681 ttggcgaact gcgaggtgac gcagccctcg atggacgcgt ggccggagtt gtgggggcgg
     4741 gccgccagct cgttcacaag cagggcgccg tcttcgacga acagctccac cgcgagcagg
     4801 cccacgacgc cgagccgctc cgccacgtcc agcgcgaggc gccgcgcctc gccggccagc
     4861 gcgtggctca ccggcgcggg gtagcgcagc tcgcgcagca tcccgtcgcg ctgcaccgtc
     4921 tggaccaccg ggtaggcgac ggcctcaccg gacgggcggc gcgcgaccac gaccgcgacc
     4981 tcgcagtcca tcgcgacatg acgctcggcc agaagcacca ggccgcggcc gtgcgcctcg
     5041 tcgagaacgg ccgccgcctc gtcgatgccg gcggccaccc acacgccccg gccgtcgtag
     5101 ccgcctcgcg cggccttgag caccagcggc cagccatgcg cctcaccgaa cgcggccatc
     5161 tcctcgcgcg agcccaccac gcggtgctcc ggctgcggcg cgcccaggcc ggccagcgcc
     5221 tcgcgctgcc ggcgcttgtc gatcaccacc gcgatgacac cggagcccgg ccggaccagc
     5281 gcgccgtcgc gctccagcgc ctggatcagc gccggttcca ccagctcgtg gtcgaacgtg
     5341 accgcgtcgg cgcccttggc cagcgccgcc aggccgtcag ggtcgtccgg cgcgccgagc
     5401 accacctcgg cggccacggc ggtggcgctg tcggccgggt cggacgccag cacccggagg
     5461 cggacatcca gcgcgacggc cgcctgcgcg gtcatacgcg cgagctggcc cgcgcccacc
     5521 atgccgacca ccggcgccac acgacctcct cgcagactcg aagacgcgcc gatcctagcc
     5581 gcacgctagg gtccgcccat gcgagcggag cccgagatca cgtgcgtgga ctgcggaggc
     5641 acgtgccacc tgctcaacgc cgatcccctg gaggacctgc cggagggctg gatcgtcgcc
     5701 taccgctgcg aggactgcct ggaccgctgg gacctggtcc tggaggagcc cgaacagcgg
     5761 tgagggccgg tcagcgaccg gccctcacca ttttgctcac gtgggtcaac tgatcgtgcc
     5821 gccgtgctcg ggtgacggcg acgagtccgc ctgggtgaag ccggactggc cggccgggcg
     5881 gatcgcgtcg tacgtcgccc cggccacctc cgcgccgatc gccgcgttgt acgcgacggc
     5941 gctgctcgcg ctcaccgtgc agtccacgtc gtcgttgccc gcggtggcca cgaccgagca
     6001 gccggcgagc tgcacgcgct cgccggcgtc cgtgtacacg tggtacgtgc ccggtccgcg
     6061 gtcgaccgtg ccgctatagg tgaaccggat ggtcaccgac gcggtgctgc cgctgccgga
     6121 cgtggagcgt gtgatgccgg tcagccgcgg cgccgccgtg ttgccggtcg ttgtggaccc
     6181 tggcgccatg ggctcctcgt cggcaccgtt gaacaatccg gactcaagga gcgacccctt
     6241 caccgcttcg tcctcgacga agccgcccac gatgacattg cgcagggcgc cggcgtcgaa
     6301 cgtgacgagg acgcgcgtga tggtcgtgcc ctgcgtcgcg tccggtgccg ccccggtgcc
     6361 gctgaacacc cggccgcgcg agtcgtagac cttgaatctg gaggcggcct ggttcacgtt
     6421 cacggccgtg tcgatctgct ggtcgaacgt gaagcgcatc tgcacggtgc tgccgctctc
     6481 gcgtgcgacg ccggtcaggt ccggcccctc cgtcgcgccg acgtgcgcgc ccccgggcgg
     6541 gttggcgtac cccccggcca cggccgtcac ggctgcggcg tccacgacgc cccgcccgac
     6601 gttgcccgac cacgtgtcgg tcgtcatgtt gtcgtcgtcg tgccaactgg aggcgagttg
     6661 gacctggcag gtgaggtcgt caaccttcac ggcgtcgccg ctcgtgccgc ggatcacctc
     6721 cacaccagtg tccccggtcg cttgcttctg cagcacgagc cggtacttgt tgttcgggcc
     6781 gcaggccgcg accggcttgt cgaacgtgaa gttgacctcg ttgtcgtcct cctccgccac
     6841 cgacaccagg atcggtccgc gcgagtcgcc ggaagccggc gtcaccgact gcaggcctac
     6901 ggagccttcc gcgttcggca cgttggaggt gagcccgcgc ccggcctcga acgccacggc
     6961 ggccaccgtc gcgtcgtcgg ccaaggcctc cgggaaggtg gcgaccacga cgtcctggcg
     7021 cgcggtgtcg cgggcggcgg tcgtggcggg cacctggagg ttgtcgaagg tgtacagccg
     7081 gaacttgtcc gcgctgacct gcgccgccag gggctcggtg aaggtgaagg agacctccgt
     7141 ggcgccggcg gccgtggtgc ccagcgtggc ggcgagcagc ctcggcgtcg aaccggtgat
     7201 cggcgtgtgg cccatcgcct ccgaggccgt gtcgcacgcg tcacggttga ccttcggcgc
     7261 gcacgtcagc tcggtcgccg cggccttgcc cgacggcgcc agccacgtgc cagtgggcgc
     7321 cggcacgctg gagccgttgg ccagcacgat cggcgcgtcg ttggccgccg agtacgcggc
     7381 ggcggtgaat ccggccgccc aggcgtcgga ggcctggccc tccgtcagga tggtggccga
     7441 ggccttggag gcgtccgagt agccgcgctc cttggcgatc tcgaccgccg tgtcgaagcg
     7501 cgtcgggccg gacacgcgct cgaccgtcat gccgatctgc tgcaactcgg tgatgaccgc
     7561 gtcgttgatg gcggagctgc cgcccaccac gaagaccgtg cggatctgcg ccccacgcag
     7621 gtagttcttg gtggaggtcg acagccgctg gctctccgtg agcagcaccg gcatgttgcg
     7681 gtcggccgcc cagccgcccg cggcgatgga gtcggcgaac gcgcgcgtcg ggtcggtgtt
     7741 cggcggctcg aacgcggcgg cgacgatggc ggacgtcgcg gccggcagcg cccggcgggc
     7801 gatgtcgatg gcggtctcga tgcgggtcgg cccgctgaag cggcgcacgg tgtagccctc
     7861 ggacttgagg tcgtcctcga cggccgtgga caccgcgctc ggcccgccca tgatgttgac
     7921 cacggacggc ttcaggcggt tgagctccgc gtcggcctca ctgctcagcg tttgcgaact
     7981 ggtgagcagg agcggacggc tgccctgcat gccgccggac gccagcgaat cggcgaacag
     8041 gtcgtcacgg cccaggagca cctcgctcgc cgaggtcggg aacgtcgcct tgctccacga
     8101 cgcggcgacg gccgtgttcg tggtgccgct gagctcggtg gtgttggtgt agtggctcgc
     8161 cgccgcgggt tccaccgcga ccaggagcaa cgcggccagc agcgccgcaa gcgccccggc
     8221 cagtccccgt gtccgagttc cgaacgtttc ggtgtgccgc atgcctcgca ctccccgcat
     8281 ctcgccgtcc gtgcaggtgg cacgggcggt ctcaccggcg ttacccgtcg gcgcgctgac
     8341 tgggcccgtc cgggcccgtg accattcagc ccggggatcg taggacaccg atgcctccat
     8401 gggcgtgcag gttcacaaaa tcgttgcgcc acacaattga gaacgcggcg gcccgcggag
     8461 gccttacggg gatctcgccg gcgcgcacgc cgccctcagg ccgggcggcg caccgcgacc
     8521 tgcgtcgaca cgcagccgca caccgggcaa ggggcttgcg tgccctcctt gcccaggtag
     8581 aaccaccgct cacacgaacg gcaccacgcc cacatctcgt tcactgaacc acctcgcgac
     8641 caggggttcc cccgcagtca caaagatcgg gcgcccaact cgctttcgct acccccgcgc
     8701 ggggggatcc catgccccca ttcgaggttt tcgcgtaaaa aaccgtccgt gttgtcggtt
     8761 ttgtggtgga ttggaggcgg tgcggcggcc gtcgtgcgtg ctcagcgagc cgccagcgca
     8821 cctgccaggt cggccgccat cgcctcgatg cgccgggcca ggccggcgcg cagggacgcc
     8881 tcgtcgccgg cgggctcgcc cttcgggtac gtgatcgttc gcgccgcgtt gaccagcgcc
     8941 cccctgccct ctgcgtcgaa cgccgcgacg gcgtcggcgg ccccgccccc ctgtgcgccg
     9001 tagccgggga ccagcacgat ggcgcgcggc atgcggtccc ggagcgcgcg ggcctccgcc
     9061 gggtacgtgg cgccgaccac ggcgcccacc gcgctgtagc cccgcgcccc cgtcagcgcc
     9121 tcacccgctt cggcgaccat gtcggcgacg cgctcggcga tcgtgcgccc gtcgtcggtg
     9181 cgcaggtcct gcaggtcgcc gctgcccgga ttggacgtct tgaccaggac gaagatgcct
     9241 ttgccgtgct cggcgcaggc gtccacgaac ggccgcaggg tgtcccggcc caggtacggc
     9301 gtgacggtca gcgcgtcggc cgcgaaggcc ggccggcggg cgccgaacac gtcggcggcg
     9361 cccaggaacg cgttggcgta cgccgccgcc gtggagtcga tgtcgttgcg tttcgcgtcg
     9421 gcgatgacca gcaggccgcg ctcccgggcc atgtccagcg tgtcggcgaa ggcctgcagt
     9481 ccctcgatcc cgtactgctc gaagaaggcg atctgcggtt tgaccgcggc gacccggccc
     9541 gcggcctcgt cgagcacgat ctcgtggaag gcgcggatgg cctcgcgcac cggctgggcg
     9601 gcgcggcgcg cggcgtccag caggaacggc ggcatcgact cgaggcgggg gtccaggccg
     9661 acgacggcgg gggtgccgca gccgtcgacg gcgtccagga ggcggtcggc gaagttctcg
     9721 gtcatcggct gcgaacccta cggcacccga gccggctagt aggtgacgct cacctccggc
     9781 gagcaggtgc cggtgccggc ctcgcagacc tggaatcggt gcgtcccgcc gcccttgctc
     9841 ttgaggttca cggtggccgc accgtcgttg gccgtggttg cggccttcgc cccgtcgagg
     9901 tggatgtcca catcggtcga cgtggcgccg gtccacgcga ggtcggcgtg cttgacgccc
     9961 ttgatcttgt agccggtcgc gctgagcgag atgccggccg gctccggcgc cggctcggtg
    10021 tccgggttgt cgaccgtgac gaccacggac gcgctcgccg tctgcggcgc ggcgccctgc
    10081 tcggtggccc tggcggtcag cgtgtgcgcg ccgtcggcga cggcggtgct gtcccacgac
    10141 gccgaccagc cgtcggtgcc gtcggtgtcc gtcgcgaacg gcgtggtggc gccgttcgcg
    10201 aagaactcca ccttggccac gccctcggtg tcggcggcgt ccgccgtcac cggcaccgcg
    10261 ccggagacgg tcgcgccctc ggcggggctc gtgatcgcga ccagcggcgc ggggtcgagc
    10321 accggcccgg cggtcgcggg cgcccacggg cgcgcatcca ggaagggctc ggcgtgcggg
    10381 ttgccgctgg agttgctcca actcgaagtg gcgtcggcct cggtgagcat ggcgccgcgg
    10441 gccgcggcga acgcgccggc gtcggcgggc tgcgggttgg ccgccaggta cagcgccaac
    10501 gcgccggcga cgtgcggcgc cgccatggac gtgccgctca tcgaggtggt gccgccgccg
    10561 cgcctggtcg acacgacgga gcggccgggc gcggccaccg cgacgggcgc ggaggctgcc
    10621 gaggcccacg acgcggcgtc gttgccccag ttggaccagt acggccagtc gtcgccctcc
    10681 tgcgtggccg acacggtgac caccgtgtcg tcgtaggcgg ccgggacggc tcccgacgcg
    10741 tcggcgtcgc tgttgccggc ggcgacggcg aagaccgcgc cgccgtgcgt cgcgttgcag
    10801 atggactcgt ggtaggcgtc ggagccggtg aagccggaag cggtgcaggt gcccgtcttg
    10861 gagccggtgc cgcccaggct catgttcacc acgcccgcct ggccgcgcgc ggcgatctcc
    10921 ttcgcggccc agtcgacgcc cgcgatgacg ccggagcggc ggccggagcc gttcttgtcc
    10981 aggaccttca cggcgtgcag cgtcacgccg ggcgccacgc cgacgacgtc gatgtcgttg
    11041 tcggcggcac cgaccgtgcc cgcgacgtgc gtgccgtgcc cgtggtcgtc gtcccaggcg
    11101 gcgcgacagc ccccgccgcc gcagctggtg acggcgtagc cgttgcccag gttggcggcg
    11161 aggtcggcgt ggtcggcgtc gatgccggtg tccacgacgt agacgtggat gcccgcgccg
    11221 gtgttggtgg cgctgcggtc ggtggcgtcc aggccgatgc gcgcgacgcc ccagggcgcg
    11281 acctcgccga aggcccggac ggcgccgtcg cgctcgacgt ggcgcacgcg cgggtcggcg
    11341 gcaagcgcgc gcaggcggcc ctccggcacg gcggcggcga agccgaacag cgcggtgccg
    11401 taggcctgcg tggcgcggac gccgtgctca cgcgcgacct gcgcggcgcg ggccttgttg
    11461 gcgtcgtggc cgccgggcgc gtgacgggcg tccagcacca cgatcacgtc gaccgtccgg
    11521 tccgccgcgg ccgctgcggg ggcgaccagg gagaaggcga gtgagaacac cgcgcagagc
    11581 gcgccgaccg tgtgcttcac gacgtgtcct gttcccgtgt gcgcggtcgc ggggccgggc
    11641 gcgaacgccc tgctccggca accccgctgc aattgacctc ccgcccgccg cgctgcccgc
    11701 gggtcaccgc tcacggtact gctgatttgg gcgttttgat aggtcttcgg tgtttattcg
    11761 aagcgaaaga tcggcgggag cacagccacg agggcggcaa cccagagggc gaacaccggc
    11821 aggtaggagg tctgccaccg ctcgaggcga tggaacgcgg cccgtcccgt gaggaagcgc
    11881 gccgacagcc acgcggtgcc ggccaggttg accagcagga gcaggttgag ccccagggcg
    11941 gccacgcggt tcggagtcag cccgaggtcg ccgatgcgcg ccaccatcgc gcccaggacc
    12001 atgccgtcga gcaccagcgc gccggccacc gcgacgagct gcacacggtc catgagcccg
    12061 ggcgggcgcg acgggtcgcg cgccgacatc gcatagagca ccaggccgag cacgaccacc
    12121 agcagcgcgt cgaagacggc gagcagttcg cggtcgaacg cgccggccgc gccggacacg
    12181 gcgtaggcca ccgcggcgac ggcgagcatg gcggcgaaca gcggcgtgaa cagcatggtc
    12241 agcaccggcg ccatgttctc caccacctgc tgcttggctt cgaccagcca tgccgccacg
    12301 atcaccgcgc cggccgcgcc cgagggcagc acccactcga tgacccgctc ggcgagctcc
    12361 ggccccagcg gttgcaggat cgccacggtg aggcccatga gcaccccgcc gccgagcgcg
    12421 atcagcgcgt agtagatgag ccactccccg gtgaagcgca cgaagtccat gcgccgctcg
    12481 tgggagcgca gcgtgccgcc catgtacggg caggcgaccg cgaaccacag caggaccggc
    12541 aggtgcagcg cgacgagcac gtgcgtggcg gagccgtcgc ggaacggaga gaggttgacc
    12601 accagtgcgg ccagcgcgaa gggcgcggcc gaccccagcc acccccgcgc gcccagccgc
    12661 cgccgccgcg cgaagaacgc cgccaggaac ggcagcacga acaggccgag attgcggacg
    12721 aaccatgccg gcggctcgcc gggaaactcc gcggccaggc gcgcgacctg gatggcgacc
    12781 gcggcggcc
//
LOCUS       DATMKC010000284         2779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_313048, whole genome shotgun sequence.
ACCESSION   DATMKC010000284 DATMKC010000000
VERSION     DATMKC010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313048"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2161)
                     /locus_tag="VNU01_08390"
     CDS             complement(<1..2161)
                     /locus_tag="VNU01_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32676.1"
                     /translation="MAVRPAAEHEVGRGSFRLALIAATTAMILGSLPFLPRHLWPRAW
                     ELALVAGLGAALVASTALPFLRRFWAVAGGTAVLGMITLLCVVTGGPDSPYTVLYLIY
                     LLYGAIFNTRRRLVAEAVLLVAAAGLLLGLDSEPTGRDAFVAVLRLASLLIVGAVVHG
                     LVAQLRRIARRLESSEQQYRSLVEYNPDAVHTFDLQARFTSANPAAAAVSGYSTDELR
                     QMRDLPLQALLAPEAVEHTMAGFRATAEGRPQHFETVIVNRHGERVELQVATVPIVVD
                     GKVVGVYSVSKDITESKRAARLLGGALAQQAAVGRLGQSALVAADPEALLDEAASLVA
                     QTLRVPLAGVFELHGEDEAVLVAGSGWRAGVVGSAVVPATAPYDVGLARASEEPVVVA
                     DYVMADDGRPTPLLEEHHAGSAMSVALRSRDAVFGVLAVYDREPRDFTDDERTFIQTV
                     ANVLASAIGRRRAEQQLLQAQKLQAVGQLAGGIAHDFNNLLTAMLGYCELLEPRVRDH
                     PMAAKHLEEALKAGERARALVDQILTFSRGRVSSPARCDLNDTLRGLKGLLLPLLPED
                     IDLRLELDPALGALRADPTQLEQVVLNLALNARDAMPGGGELRVATANAEVDRARGDR
                     LGLPAGSYVTLTVEDAGTGMDAVTATRLFEPFFTTKADGNGLGLATVYGIVTSAGGQI
                     QVESQPGHGARFTVYLPRVAADTEPAAAAPAPERRPAR"
     gene            2465..>2779
                     /locus_tag="VNU01_08395"
     CDS             2465..>2779
                     /locus_tag="VNU01_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32677.1"
                     /translation="MLAFLLLTVACGLKEGVEVSFQQGGAGGAEDFGGGFAADPGATD
                     DFTGTDGDAPVSDGTTSDGADPGTTGAGTTGGTTGGSGTTGTTGGGGGGAGGSGAAGG
                     AAG"
BASE COUNT          359 a         1048 c         1028 g          344 t
ORIGIN      
        1 gccgggccgg ccggcgctcc ggcgcggggg ccgccgccgc cggctcggtg tccgcggcca
       61 cccggggcag gtacacggtg aagcgcgcgc cgtgaccggg ctggctctcc acctggatct
      121 ggccgccggc cgaggtgacg atgccgtaca cggtggccag cccgagcccg ttgccgtccg
      181 ccttggtggt gaagaacggc tcgaacagcc gcgtcgccgt gaccgcgtcc atgccggtgc
      241 cggcgtcctc caccgtcagg gtgacgtagg agccggcggg caggcccagg cgatcgcccc
      301 gcgcgcggtc gacctccgcg ttggcggtgg cgacgcgcag ctcgcccccg ccgggcatgg
      361 cgtcgcgcgc gttgagcgcc aggttgagca ccacctgctc cagttgcgtg gggtcggccc
      421 gcagcgcgcc cagcgcggga tccagctcca ggcgcaggtc gatgtcctcc ggcagcaggg
      481 gcagcagcag gcccttgagg ccgcgcagcg tgtcgttgag gtcgcagcgc gccgggctgg
      541 agacccggcc gcggctgaac gtgaggatct ggtccaccag ggcgcgggcg cgctctcccg
      601 ccttcagcgc ctcctccagg tgcttggcgg ccatcgggtg gtcgcgcacc cgcggctcca
      661 gcagctcgca gtagccgagc atggccgtca gcaggttgtt gaagtcgtgc gcgatgccgc
      721 cggcaagctg gcccacggcc tgcagcttct gcgcctgcag cagctgctgc tcggcgcggc
      781 ggcggccgat cgcgctggcc agcacgttgg ccaccgtctg gatgaaggtg cgctcgtcgt
      841 cggtgaagtc gcgaggctcg cggtcgtaga cggccaggac gccgaacacc gcgtcgcggc
      901 tgcgcagcgc cacgctcatc gcgctgcccg cgtggtgctc ctccagcagc ggcgtgggac
      961 ggccgtcgtc ggccatcacg tagtcggcca ccaccacggg ctcctcactg gcgcgcgcca
     1021 ggcccacgtc gtagggcgcg gtggcgggca ccacggcgct gccgaccacg ccggcccgcc
     1081 agccggagcc ggcgaccagg acggcctcgt cctcgccgtg cagctcgaac acgcccgcca
     1141 gcggcacgcg cagcgtctgg gcgaccaggc tggcggcctc gtccagcagc gcctcggggt
     1201 cggcggccac cagggcgctc tggccaaggc ggcccaccgc cgcctgctgc gcgagcgcgc
     1261 cgcccagcag gcgggcggcg cgcttgctct ccgtgatgtc cttggacacc gagtagacgc
     1321 ccacgacctt gccgtcgacc acgatgggca cggtcgccac ctgcagttcc acgcgttcgc
     1381 cgtggcggtt cacgatcacg gtctcgaagt gctgcggccg gccctccgcg gtggcgcgga
     1441 agccggccat ggtgtgctcc acggcctccg gcgccaggag agcctgcagg ggcaggtcgc
     1501 gcatctggcg cagctcgtcg gtgctgtagc cgctgacggc cgccgccgcc gggttcgcgc
     1561 tggtgaagcg cgcctgcagg tcgaaggtgt ggacggcgtc cgggttgtac tccaccaggg
     1621 agcggtactg ctgctcgctg gactccagcc ggcgggcgat gcggcgcagc tgcgcgacga
     1681 gcccgtgcac gacggcgccc acgatcagca gggaggcaag ccgcagcacg gccacgaacg
     1741 cgtcgcggcc ggtcggctcg ctgtcgagcc cgagcagcag gcccgccgcg gccacgagca
     1801 gcacggcctc ggcgacgagc cggcggcggg tgttgaagat cgccccgtac agcagataga
     1861 tcaggtacag cacggtgtac gggctgtccg ggccgcccgt caccacgcac agcagcgtga
     1921 tcatgcccag cacggcggtg ccgccggcga ccgcccagaa gcgccgcagg aacggcagcg
     1981 ccgtggacgc aaccagggcc gcacccaggc cggcgaccag cgccagctcc cacgcccgcg
     2041 gccacaggtg gcgcggcagg aagggcaggc tgcccaggat catggcggtg gtcgcggcga
     2101 tcagggcaag gcggaagctg ccgcggccga cctcgtgctc cgccgccggc ctgacggcca
     2161 tcggccgtgt caaccccccg ccgtccatcg gcgacccccc aaatcgggcc ccttcgcatc
     2221 cgccgccacg ggacccgccg tcccgctccg tcagcgagca tagcgatcac aggctccgga
     2281 tatggcctac ccggcacggg gcgcgcaccc ctgggcagcg tctcatccga cttaccggac
     2341 tcttgtgcgc gcccttgacg ccgccgtgcc ctggacgttt gctggggggt cgtcccccgc
     2401 acgtggtgga ccagccaacc ggagggtggg agtgtccatg cgcacgacgc gcacgctcgc
     2461 agtcatgctc gcgttcttgc tgctcaccgt ggcctgcggc ctcaaggagg gcgtcgaggt
     2521 ctccttccag cagggcggcg cgggcggcgc cgaggacttc ggcggcggct tcgccgccga
     2581 ccccggggcg acggacgact tcaccggcac ggacggggac gcccccgtca gcgacggcac
     2641 cacgtccgac ggcgccgacc ccggcacgac cggcgcgggc acgaccggcg gcaccaccgg
     2701 cggctccgga accaccggca ccaccggcgg cggcggcggt ggtgccggtg gttccggagc
     2761 cgccggtggt gccgccggt
//
LOCUS       DATMKC010000285         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_121826, whole genome shotgun sequence.
ACCESSION   DATMKC010000285 DATMKC010000000
VERSION     DATMKC010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121826"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VNU01_08400"
     CDS             complement(<1..1084)
                     /locus_tag="VNU01_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32678.1"
                     /translation="MKLRTRGRAALGALAIVLISASMPAGAGVIPDEVDFVDDYVDLY
                     DEENREADTPGLGIGWAIARLDVADVALSNLVDRGELRANTGDIRTTVTTVGQPYSEL
                     MFNPHRMFNDVVPPWNPKRRGLNSDPEQGTYRAVLEHPQVPDVEGLLGIGRYYVQSNA
                     KGSFAEVGSASGWMRAKRFGLVSDMGDKGLVSAVTNEAAGAKVALTVEDFQINLNDVF
                     QQEAVELPLGMTLDIATAVSLPGGHTAQKAYERLKEFEQELGVLNGLAEDLAFLRAEL
                     EPYAAAMPGYAEAEAAVEAAEAQLAEARATAKAAAEEVVRLVAENEALNTAINSVNTM
                     LAAVTQDIAMLQGHIDALNTQIADTVE"
     gene            complement(1222..1572)
                     /locus_tag="VNU01_08405"
     CDS             complement(1222..1572)
                     /locus_tag="VNU01_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32679.1"
                     /translation="MTVAQSEEYATKEDLHRVEDRLERGVTDLRAEIATARQESKADN
                     QALRAEMAAGHDRLREDITSTENRLRDDAAKESQKVWDELKALGKAIDNLRRDGMWFG
                     LTLAGVLATIEVIA"
     gene            1796..>2689
                     /locus_tag="VNU01_08410"
     CDS             1796..>2689
                     /locus_tag="VNU01_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWH32680.1"
                     /translation="MATAVDDMVSQHDAVLVDRHRAGDRRAFGILYDAHYGRLLSYIR
                     RRVREGDLVEDIAQEAFVKAFAAMGDLRETGRFYPWLTAITRRLILDHYKARVRVIAV
                     PDLDAGQSEPADEALMQRQTQADLAAALLRVRARHQQVLWLREHEGLSYQDIALRLGI
                     PETAVAPLLFRARKALQREYLAITQQERSAVVVPALLFLADAARRLRDRLAAMVPYLP
                     EPAALTSSMAAVALSFAAVVGGGAEVVDAPRLASPAPSVSGSAASAALATAVDVAPEA
                     SIRRETAQHARDGGLAPVAPYE"
BASE COUNT          340 a          950 c          967 g          432 t
ORIGIN      
        1 tctccacggt gtcggcgatc tgcgtgttca gggcgtcgat gtggccctgc agcatcgcga
       61 tgtcctgcgt caccgcggcc agcatggtgt tcacggagtt gatcgcggtg ttcagcgcct
      121 cgttctccgc caccaggcgc acgacctcct ccgcggccgc cttcgccgtg gctcgcgcct
      181 cggcgagctg cgcctcggcg gcctccaccg cggcctcggc ctcggcgtag ccgggcatcg
      241 cggccgcgta gggctccagc tcggcgcgca ggaaggccag gtcctcggcc aggccgttga
      301 ggacgccgag ctcctgctcg aactccttca ggcgctcgta ggccttctgc gcggtgtggc
      361 cgccgggcag cgagacggcg gtggcgatgt ccagcgtcat gcccagcggc agctcgacgg
      421 cctcctgctg gaagacgtcg ttcaggttga tctggaagtc ctccaccgtg agcgcgacct
      481 tggcgccggc ggcctcgttg gtgacggcgc tgaccaggcc cttgtcgccc atgtcgctga
      541 ccaggccgaa gcgcttggcg cgcatccagc cgctggcgct gcccacctcg gcgaaggagc
      601 ccttggcgtt gctctgcacg tagtagcggc cgatgccgag caggccctcc acgtcgggga
      661 cctgcgggtg ctccagcacg gcgcggtagg tgccctgctc gggatcggag ttcaggcccc
      721 ggcgcttggg gttccacggc ggcacgacgt cgttgaacat gcggtgcggg ttgaacatga
      781 gctcggagta gggctggccc acggtggtca cggtggtgcg gatgtcgccg gtgttggcgc
      841 gcagctcacc gcggtccacc aggttgctga gcgccacgtc ggcgacgtcc aggcgcgcga
      901 tggcccagcc gatgcccagg cccggcgtgt cggcctcccg gttctcctcg tcgtacaagt
      961 cgacgtagtc gtcgacgaag tcgacctcgt ccgggatgac gccggcgccc gcgggcatgg
     1021 acgccgagat caggacaatg gcgagcgcgc caagcgccgc ccgcccccgc gtacgcagct
     1081 tcacaccgtt catagcggat acatcggggg ttacggatgt gcggtacgta ggtcaggccg
     1141 gggacggccc acccccgact cggtgacgca caaacccgga cagacctgtt ggagtgggtc
     1201 gcgcgcggtg ccgctcgacg ctcaggcgat gacctcgatg gtggccagca cgccggccag
     1261 cgtcagcccg aaccacatgc cgtcgcgtcg caggttgtcg atcgccttgc ccagcgcctt
     1321 gagctcatcc cacaccttct gggactcctt ggcggcgtcg tcgcggaggc ggttctcggt
     1381 ggaggtgatg tcctcgcgga ggcggtcgtg gccggcggcc atctccgccc ggagtgcctg
     1441 gttgtccgcc ttgctctcct gccgggccgt cgcgatctct gcccgcaggt ccgtcacgcc
     1501 gcgctcgagg cggtcctcca cgcggtggag gtcttccttg gtcgcgtact cctctgactg
     1561 tgccacggtc accgcgcctc ctcatggccg cactgccgct cgggttccca cgaccgtaac
     1621 gcccccgcgc cgcagggggc cgaaatcgcg cgctcccgtt gtggacaacc ggccgtgacc
     1681 gttccgtccg cagtcgcccg gcgacgattc gggagcaatc agacggcctt taccgggagg
     1741 attacggggc gtacagtgcg ccgcggcgga agcactggac acgggggtcc gcgcggtggc
     1801 gacggcagtc gacgacatgg tgagccagca cgacgccgtg ctggtggacc gccatcgcgc
     1861 cggcgaccgc cgcgccttcg ggatcctcta cgacgcccac tacggccggc tgctgtccta
     1921 catccgccgc cgcgtgcggg agggcgatct cgtcgaggac atcgcccagg aggcgttcgt
     1981 caaggcgttc gcggcgatgg gcgacctgcg cgagacgggc cggttctatc cgtggctcac
     2041 cgcgatcacg cgacggctga tcctggacca ctacaaagcg cgcgtgcgcg tcatcgccgt
     2101 gccggacctg gacgcggggc agagcgagcc ggccgacgag gcgctgatgc agcggcagac
     2161 gcaggcggac ctggccgccg cgctgctgcg cgtgcgcgcc cggcatcagc aggtgctgtg
     2221 gctgcgtgag cacgagggcc tgagctatca ggacatcgcg ctgcggctgg gaatccccga
     2281 gaccgcggtg gcgccgctgc tgttccgcgc gcgcaaggcg ctgcagcgcg agtacctggc
     2341 gatcacgcag caggagcgct cggccgtggt ggtgccggcg ctgctgttcc tggcggacgc
     2401 tgcgcggcgg ctgcgcgacc ggctggccgc catggtgccg tacctgcccg agcccgccgc
     2461 cctcaccagc tcgatggccg ccgtcgccct cagcttcgcg gccgttgtcg ggggcggtgc
     2521 ggaggttgtc gatgctccac gcctggcgtc tccggcgccg tccgtttccg ggtccgccgc
     2581 cagcgcggcc cttgcgacag cggtggacgt cgcgccggag gcttcgattc ggcgggagac
     2641 ggcccagcat gcgcgcgacg gaggactggc acccgtcgcg ccatatgag
//
LOCUS       DATMKC010000286        12105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_9230, whole genome shotgun sequence.
ACCESSION   DATMKC010000286 DATMKC010000000
VERSION     DATMKC010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9230"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /gene="der"
                     /locus_tag="VNU01_08415"
     CDS             complement(<1..877)
                     /gene="der"
                     /locus_tag="VNU01_08415"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWH32681.1"
                     /translation="MSAGGRTVRELGGPVVAVVGRPNVGKSTLVNRLTGRRDAIVEQR
                     PGVTRDRTTHAAEWRGRTFTVMDTGGWLPGWAQRGRQTGDDLDALVSAQAERATQTAD
                     LVLFVVDLTVGITEEDAAAAEWLRGAGLPVLLVANKADTTRVEPELAELHGLGLGEPL
                     PVSALHGHGSGDLLDAVFDGLKKVGAFQRKRVPVDDIPAVALIGRPNVGKSSIFNRIL
                     GEERTIVDDRPGTTRDAVDTLVELEDGRTYRFIDTAGLRRKTRKNDATEYYSTVRTVQ
                     ALDAACVALLVVDASE"
     gene            complement(874..1509)
                     /gene="cmk"
                     /locus_tag="VNU01_08420"
     CDS             complement(874..1509)
                     /gene="cmk"
                     /locus_tag="VNU01_08420"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWH32682.1"
                     /translation="MTVVAIDGPAGSGKSTVAAALALRLGVPHVDTGAIYRAATLAVL
                     RAGVDVRDEAACLRVVERASILRADGRTRLDGEDVEALIRGPEVTAAVSAVSEHPAVR
                     AALLGLQRAQVGDAGGVVEGRDAGTVVVPDARLKVWLTANPLERARRRAAERGETDPE
                     AVAAQAADLERRDAHDAAQMRRAPDAVEIDTTGRGIEDLVDELASMVEARA"
     gene            complement(1506..2810)
                     /gene="aroA"
                     /locus_tag="VNU01_08425"
     CDS             complement(1506..2810)
                     /gene="aroA"
                     /locus_tag="VNU01_08425"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWH32683.1"
                     /translation="MTGRELPDAAVRVQPGRAAGALAAPGSKSVTNRLLVLAALAEGD
                     SALRHPLDSDDTRAMRRLVAALGADVRDGEGAWHVRGTGGRLSAPGAPVDVLLSGTTM
                     RFGAALATLADGPVELTGGPSLLRRPIAALTGALRDLGADVRDRDGHPPVRTGGGGLR
                     GGPVRVDVGASSQFASAVLLVAPYAREADVELQVHGEAALSYVELTAEAMRDWGARVG
                     GGDGRWLVARGGYTARDVEVDYDASAAAHLWTVAAASGGAVTVTNVAERTLQADAAFP
                     RLLAAMGCGVQQDGSALTVTGPPRLRGLGEWDLGAMPDQLPSAAVLAAVADGETVLRN
                     VAVVRGHETDRIAALAGELAKAGVDAEELPDGLRIRGGVPELAAPVRLDTHDDHRLAM
                     AFAALAARVPGIVIEEPWCVRKTYPRFWADLARLGLRWEAAA"
     gene            complement(2807..3910)
                     /locus_tag="VNU01_08430"
     CDS             complement(2807..3910)
                     /locus_tag="VNU01_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWH32684.1"
                     /translation="MRVAIIGTGLVGGSLGLALRKHAAQAVERVVGYDAAQDAAAQAA
                     ERDVVHDWAATPRDAVADADVVFLAVPVSEIPRMAEEVGPAMRQGAILTDVGSVKGGL
                     VDAMQRAVPDGVHVIGGHPMAGSAETGAAHASPDLFVGATYLLTPTTHTDPVAYQRLH
                     GLVAALGARVLAVGPDRHDLLVAVVSHLPQLAASTLMNLAAERARSEHAGLLLLAAGG
                     FRDATRVAASNPDLWLDICAENRDAIVAVLADYEERVRALRAVLQGADDDGLRRLLSQ
                     ARDARRTLPGKETPAGELVELSLPIPDRPGVLAEVTTTVGGAGVNIEDFGIEHSPEGG
                     RGRLRMAILGAAQAELARQALEARGYEVSELSS"
     gene            complement(4005..4385)
                     /gene="aroH"
                     /locus_tag="VNU01_08435"
     CDS             complement(4005..4385)
                     /gene="aroH"
                     /locus_tag="VNU01_08435"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWH32685.1"
                     /translation="MIEGFRLAALRGATTLESDSRDQVLERTAEMLQALMERNGLSPD
                     DMVSLILTATDDIRSEFPAAAVRAAGISDVPMLCARELDITGGSGVPLCIRLMAHVYT
                     DRPRAELHHVYLHGARQLRSDLPE"
     gene            complement(4382..5176)
                     /locus_tag="VNU01_08440"
     CDS             complement(4382..5176)
                     /locus_tag="VNU01_08440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWH32686.1"
                     /translation="MTSEHPDTSGGKAERVQKILAAAGLGSRRACEEVIAQGRVAVDG
                     QVITLGAKADPVTQVITVDGERIHTNPGLVYLLLNKPQGVVTTVTDPQGRPTVMDLVP
                     VNPRVYPVGRLDRDTEGLLVLTNDGELANRLAHPRWQVEKTYVAQVRGTVKRHQLRTL
                     LDGVELEDGLAKARSVRELGSAQDRTLLEIVLTEGRKREVRRMLAAVGMNLERLARVK
                     IGPLPLGDISPGKSRPLTGAEVRALYASVGLGAPESGDSGSRRAAS"
     gene            complement(5173..5892)
                     /gene="scpB"
                     /locus_tag="VNU01_08445"
     CDS             complement(5173..5892)
                     /gene="scpB"
                     /locus_tag="VNU01_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWH32687.1"
                     /translation="MTDPANSPVPAQSVLLDDELRKALEAILLVVEEPVDANTLAQVL
                     EAPTEQVVATLHALRSEYVDAGRGFVLREVAGGWRYYTDPGAAPYVERFVLNGRSGRL
                     SQASLETLAIIAYKQPVTRAQVSQIRGVDADGAVRSLLARGLVEEIGRAELPGQPLLY
                     ATTASFLERLGLSSLDELPKLPELAPAGPPPEEPPPGGYKAARRELDALARGDVADGE
                     QLAMRDEEPAGDAPAAAEERW"
     gene            complement(5889..6713)
                     /locus_tag="VNU01_08450"
     CDS             complement(5889..6713)
                     /locus_tag="VNU01_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HWH32688.1"
                     /translation="MATTYHVTVGSFEGPFDLLLHLIARRKVEIYDIPIAQITDDYLA
                     VLRQLDELDLEVATEFLVVAATLIELKAARLLPSEDDPELEELALEARDLLYARLLDY
                     RTFKEAAGWVSERMEAFTGYAPRELLGRDGQLHVRQQVAVALVQADLARLALRALTER
                     PEPVVDLSHVQPVRMTVREAAGMVMDELDRAGGRATFRELTGGCRHPIEVIVHFLALL
                     ELYKLGHVELEQAASFGEIGVELRADEPWHGSVFATMEAYDARAAEDPEDAEVPKP"
     gene            complement(6725..8083)
                     /locus_tag="VNU01_08455"
     CDS             complement(6725..8083)
                     /locus_tag="VNU01_08455"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HWH32689.1"
                     /translation="MRLRAVDLVARKRDGGELSAAELDWLVRGYLDGAVMEGQMAALL
                     MAGVLRGFSDDEAIALTDVLLRSGDRMDLSGVPGPTVDKHSTGGVGDGTTLLVAPLLA
                     AAGCTLVKLSGRGLGHTGGTLDKLEAIPGLRTALDPDEARRIAEEVGCVVAAQSERLV
                     PADKALYALRDVTATVPSVALIASSVMSKKLASGAAHIVLDVKAGDGAFMPDEAAAAA
                     LAELCVRIGTAAGRRTVALVSAMDQPLGTGIGNALEVAECVRLLREEPRGRLAELALD
                     LASAALMRVRAGASGSAGGDAGADGARDELRALWRVGQALERLRAMIAAQGGDPAVCD
                     DPGAVLPRAPVVVEAPAPRGGVVEAVPARAVGELSSQLGAGRARKEDAVDPAVGVELR
                     VEIGDRVEAGQPLAVVHARGRDDADMAAARLAEIVRLVDRDVPRPPTLLRTIVGQEEH
                     TR"
     gene            complement(8097..9014)
                     /gene="xerD"
                     /locus_tag="VNU01_08460"
     CDS             complement(8097..9014)
                     /gene="xerD"
                     /locus_tag="VNU01_08460"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HWH32690.1"
                     /translation="MVPVALPQHARRYLDYLRVERGLSRHSLVAYERDLAVYGRYLAG
                     IGTDDPLTATDGDLAGFVAWLRDATTAAGKPYAASTIARTLVSVRGLHRFLVREGLAR
                     FDPSTELSGPRPKRPLPKALHLEEIERLLASPVGDEPVALRDRAMLELLYAAGLRITE
                     LVELDVDDVDLAARSVRCLGKGRKERIVPIGRVAAAAVDAWLVRGRPALRAAGPALFV
                     NRRGGRLTRQGGWKIVKRHAEDAGLGERVSPHTLRHSFATHLIDRCADVRVVQELLGH
                     ASVNTTQVYTLVSGSRLRSVYERAHPRAR"
     gene            9123..9344
                     /locus_tag="VNU01_08465"
     CDS             9123..9344
                     /locus_tag="VNU01_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32691.1"
                     /translation="MSMPLARGADDMYADDDDRPATKKDLRVLGAELRAELHQVRADL
                     LDTISSRTTTLFFSQIAAVAAVAAIAFAD"
     gene            complement(9347..9901)
                     /locus_tag="VNU01_08470"
     CDS             complement(9347..9901)
                     /locus_tag="VNU01_08470"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWH32692.1"
                     /translation="MSDVTVTASERPFSGKLSNIRVDTVRFADGSQAEREIVEHPDAV
                     AVVALDAQGRVLLLRQYRHPVGERLLELPAGKLDRQGEDPADAARRELEEETGWSAGR
                     LDPLLSFHNSAGWTDEGTTLYLADDLRETGTPDGFTAAHEEADMEVVWVPLDEAVAMV
                     AAGEIRDAKTVIGILLTRLQRGVH"
     gene            complement(9933..11258)
                     /locus_tag="VNU01_08475"
     CDS             complement(9933..11258)
                     /locus_tag="VNU01_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWH32693.1"
                     /translation="MSEAMGAGEGNLRRDIGFLGLLALSVGINIGGGLFVLVNIAAAE
                     TGPSLPLAMALSAIPALLAIVPYRVLARAYPTAGASYRYMKLWSPRGAYMAAAALLLS
                     IVIGGQPLFALMTGEYLARLSWVSWPAETLAVAVLVVFFVLNLLGVRLAMAVQSVLLF
                     VLVGALVLFVAMGWGDVEAARFADPLPGGAMGLVTATVLLYALLAGGLFVVEVGAEVV
                     DPGRVFGAVLPLGMLIVLALYVGITVVAVGAVPWQTMDGTTLVEVAQAFMSPGAVSVF
                     ILGGAVVAGVTTINGVFALVSRGMLVVAEEGLFPRSVGTVNRRFGTPHVALSVCFAGS
                     LLSLLAGPDKTFLGVLMNLGLVVAIAAVCLAAARLPTRHPDLFAKGAGGLKPRTLRVV
                     STAVVAMNVVVALALASEAPLPALLLVLVAALAGAWHARVHRAALLPSA"
     gene            complement(11255..>12105)
                     /locus_tag="VNU01_08480"
     CDS             complement(11255..>12105)
                     /locus_tag="VNU01_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32694.1"
                     /translation="EQRDLALELRRQRAVGPAHHGVDVEIEWVPADDLSDADAAERAL
                     RGVHGVLVPGGFGVRGIDGKINAARFARERDTPYLGICLGLQVAVVEFARHVLGLAEA
                     NSSEFDPHTPDPVVDLMADQRDVTDMGGTMRLGLYPAKLVQGTRAAAAYGEPVVYERH
                     RHRWEVNNRYRPRLAEAGMAFSGLSPDDRLVEFVEIPGHPWFVATQAHPEFRSRPNRP
                     HPLFRDFVGAARERMLAQAGRLPEPSARAEAEPSARAEPEPSARAEVEASARIVVDAT
                     ETAPAP"
BASE COUNT         1529 a         4670 c         4402 g         1504 t
ORIGIN      
        1 gctcggaggc gtccaccacg agcagcgcca cgcacgccgc gtccagcgcc tggacggtgc
       61 gcaccgtcga gtagtactcg gtcgcgtcgt tcttcctggt cttgcggcgg aggcccgcgg
      121 tgtcgatgaa gcggtacgtg cggccgtcct ccagctccac cagcgtgtcc accgcgtccc
      181 gggtggtgcc gggacggtcg tcgacgatgg tgcgctcctc ccccaggatg cggttgaaga
      241 tcgaggactt gccgacgttc gggcgcccga tcagcgcgac cgcggggatg tcgtcgaccg
      301 gcacccgctt gcgctggaag gcgccgacct tcttcaggcc gtcgaacacc gcgtcgagca
      361 ggtcgccgga gccgtggccg tgcagcgcgc tgaccggcag gggctcgccg aggcccagcc
      421 cgtgcagttc cgccagctcc ggctccacgc gcgtggtgtc ggccttgttg gccaccagca
      481 ggaccggcag gccggcgccg cgcagccact ccgccgcggc ggcgtcctcc tcggtgatgc
      541 cgacggtgag gtccaccacg aacagcacca ggtccgcggt ctgtgtcgcc cgttccgcct
      601 gcgccgacac cagcgcgtcc aggtcgtcgc cggtctggcg cccgcgctgc gcccagccgg
      661 gcagccagcc gccggtgtcc atgaccgtga acgtgcgtcc ccgccactcg gcggcgtggg
      721 tggtgcggtc ccgggtcacg ccgggacgct gctccacgat ggcgtcgcgc cgccccgtca
      781 ggcggttgac cagcgtggac ttgcccacgt tgggccgccc gaccacggcc acgaccggcc
      841 cgccgagctc gcgcaccgtg cggccgcccg cgctcacgcc cgtgcctcga ccatgctcgc
      901 caactcgtcc accaggtcct cgatgccccg tccggtcgtg tcgatctcca ccgcgtccgg
      961 cgcgcgccgc atctgcgcgg cgtcgtgcgc gtcgcgccgc tccagatccg ccgcctgcgc
     1021 ggcgaccgcc tcggggtcgg tctccccgcg ctcggcggcg cggcgccggg cgcgctccag
     1081 cgggttcgcg gtcagccata ccttcaaccg ggcgtccgga accaccacgg tgcccgcgtc
     1141 ccggccctcc acgaccccgc cggcgtcgcc gacctgggcg cgctgcaggc ccaggagcgc
     1201 ggcgcgcacg gcggggtgct ccgacaccgc cgacaccgcg gcggtgacct ccgggccgcg
     1261 gatgagcgcc tcgacgtcct cgccgtcgag ccgggtgcgc ccgtcggcgc gaaggatgct
     1321 cgcccgctcg acgacgcgca ggcacgcggc ctcgtcgcgc acgtccacgc cggcgcgcag
     1381 caccgccagg gtcgcggcgc ggtagatcgc gccggtgtcc acgtgcggca cgcccaggcg
     1441 cagcgccaac gccgcggcca cggtggactt gccggagccg gccggcccgt cgatcgcgac
     1501 gaccgtcatg cggcggcctc ccagcgcagc cccagccgcg cgaggtccgc ccagaagcgc
     1561 gggtaggtct tgcgcacgca ccagggctcc tcgatcacga tcccgggaac gcgggcggcc
     1621 agcgccgcga acgccatcgc gaggcggtgg tcgtcgtggg tgtccagccg caccggtgcc
     1681 gcgagctccg gcacgccgcc gcggatgcgc agcccgtcgg gcagctcctc cgcgtccacg
     1741 ccggccttcg ccagctcgcc ggccagggcg gcgatgcggt cggtctcgtg cccgcgcacc
     1801 acggcgacgt tgcgcagcac cgtctcgcca tcggccacgg ccgccagcac ggcggcgctg
     1861 ggcagctggt ccggcatggc gcccaggtcc cactccccca gcccgcgcag ccgcggcggc
     1921 ccggtcacgg tgagcgcgga gccgtcctgc tgcacgccgc agcccatcgc cgccagcagg
     1981 cgcgggaagg ccgcgtccgc ctggagcgtg cgctcggcca cgttggtgac cgtcacggcg
     2041 ccgccactcg ccgccgccac ggtccacagg tgcgccgcgg cgctcgcgtc gtagtccact
     2101 tcgacgtcgc gggcggtgta gccgccccgc gccacgagcc agcggccgtc gccgccgccg
     2161 acccgcgcgc cccagtcgcg catggcctcg gcggtcagct cgacgtagga cagcgccgcc
     2221 tcgccgtgga cctgcagctc cacgtcggcc tcgcgcgcgt acggcgccac cagcagcacg
     2281 gcgctcgcga actggctgct ggcgccgacg tccacccgca ccggaccgcc ccgcaggccg
     2341 cccccgccgg tgcgcacggg cgggtggccg tcgcggtcac ggacgtcggc gcccaggtcg
     2401 cgcagcgcgc ccgtcagcgc ggcgatgggg cggcgcagca gcgacggccc gccggtgagc
     2461 tccaccggcc cgtcggccag cgtggccagc gccgcgccga agcgcatcgt ggtgcccgac
     2521 agcagcacgt cgaccggggc gcccggcgcc gacaggcgcc cgcccgtgcc gcggacgtgc
     2581 cacgcgccct caccgtcgcg cacgtccgcg ccgagcgccg cgaccagccg gcgcatggcg
     2641 cgcgtgtcgt cgctgtcgag cgggtggcgc agcgcgctgt cgccttcggc cagcgccgcc
     2701 aggaccagca ggcggttggt gacgctcttg ctccccggcg ccgccagggc gccggcggca
     2761 cggccgggct ggacgcgcac ggcggcgtcc ggaagctccc ggccggtcat gacgacagct
     2821 cggagacctc gtagccgcgc gcctccagtg cctggcgcgc cagctcggcc tgcgcggctc
     2881 ccaggatcgc catgcgcagg cggccgcgcc cgccctccgg cgagtgctcg atcccgaagt
     2941 cctcgatgtt cacgccggcg ccgcccaccg tggtcgtgac ctccgcgagc acgccggggc
     3001 ggtccgggat cggcagggac agctcgacca gctcgcccgc gggggtctcc ttgcccggca
     3061 gcgtgcggcg cgcgtcacgg gcctggctca gcagccgccg caggccgtcg tcgtcggcgc
     3121 cctgaaggac cgcgcgcagc gcccgcacgc gctcctcgta gtcggccagc acggcgacga
     3181 tcgcgtcgcg gttctccgcg cagatgtcca gccacaggtc cgggttgctg gccgccacgc
     3241 gcgtggcgtc gcggaagccg ccggcggcca gcagcagcag gccggcgtgc tcgctgcgcg
     3301 cccgttccgc cgccaggttc atcagggtgg aggcggccag ctgcggcagg tggctgacca
     3361 cggccaccag gaggtcgtgg cggtccgggc ccacggccag cacgcgcgcg cccagcgccg
     3421 cgaccagccc gtgcaggcgc tggtaggcga ccgggtcggt gtgcgtggtc ggcgtgagca
     3481 ggtaggtcgc gcccacgaag aggtccgggc tggcgtgggc ggcgccggtc tcggccgagc
     3541 cggccatcgg atggccgccg atgacgtgca cgccgtcggg cacggcgcgc tgcatcgcgt
     3601 cgacgagccc gcccttcacg ctgcccacgt cggtgaggat ggcgccctgg cgcatggcgg
     3661 ggcccacctc ctcggccatg cgggggatct ccgacaccgg cacggccagg aacaccacgt
     3721 cggcgtcggc cacggcgtcc cgcggagtgg cggcccagtc gtggacgacg tcgcgctcgg
     3781 cggcctgcgc ggcggcgtcc tgcgcggcgt cgtagccgac gacgcgttcc acggcctggg
     3841 cggcatgctt gcgcagggcg agcccaagag agccgcccac caggccggtg ccgatgatcg
     3901 ccacgcgcat gggccccagg ctaccgggcc tccggagcgg ccccgtgacg ggcggcctgc
     3961 ccggtcggcc ggagggcgcg acttggccca gcggccgaag gccgctattc aggcaggtcg
     4021 ctgcgcagct ggcgggcccc gtgcaggtag acgtggtgca gctcggcgcg aggccggtcg
     4081 gtgtagacgt gggccatgag ccggatgcac agcggcacgc cgctgccacc ggtgatgtcg
     4141 agctcccggg cgcacagcat cggcacgtcg gagatgccgg cggcgcgcac ggcggcggcg
     4201 gggaactcgc tgcggatgtc gtcggtggcc gtcaggatga ggctcaccat gtcgtccggg
     4261 gacaggccgt tgcgctccat gagcgcctgg agcatctccg cggtgcgctc cagcacctgg
     4321 tcgcggctgt cggactccag cgtcgtggcg ccccgcagcg ccgccagtcg gaacccttcg
     4381 atcacgacgc cgccctgcgc gacccgctgt cgccggactc cggcgccccc agcccgacgg
     4441 acgcgtacag cgcccgcacc tccgccccgg tcagggggcg ggacttgccg ggcgagatgt
     4501 cgccgagcgg gagcgggccg atcttcacgc gggccaggcg ctccaggttc atgccgaccg
     4561 ccgcgagcat gcgccgcacc tcgcgcttgc gtccctcggt cagcacgatc tccagcagcg
     4621 tccggtcctg cgcgctgccg agctcgcgca ccgacctggc cttggcgagc ccgtcctcca
     4681 gctccacgcc gtccagcagc gtccgcagct ggtggcgctt gacggtgccg cgcacctggg
     4741 cgacgtaggt cttctcgacc tgccagcgcg ggtgcgccag ccggttggcc agctcgccgt
     4801 cgttggtgag cacgagcagc ccctcggtgt cgcggtccag cctgccgacc gggtacacgc
     4861 gcgggttcac cgggacgagg tccatcacgg tgggtcgccc ctgcgggtcg gtcacggtgg
     4921 tgaccacgcc ctgcggcttg ttcaggagca ggtacaccag gccggggttg gtgtggatgc
     4981 gctcgccgtc gacggtgatg acctgggtga ccgggtccgc cttggcgccc agcgtgatga
     5041 cctggccgtc gaccgccacg cggccctgcg cgatgacttc ctcgcaggcg cggcggctgc
     5101 ccagccccgc ggcggccagg atcttctgga cccgctccgc cttgccgccg gaggtgtccg
     5161 ggtgctcgct ggtcaccagc gttcctccgc ggcggcgggc gcgtcgccgg ccggctcctc
     5221 gtcgcgcatg gcgagctgct cgccgtcggc gacgtcgccc cgcgccagcg cgtccagttc
     5281 ccggcgcgcc gccttgtagc cgcccggcgg cggctcctcc gggggcgggc cggcgggcgc
     5341 cagctccggc agcttcggca gctcgtccag cgacgacagg cccagccgct ccaggaagct
     5401 cgccgtggtg gcgtacagca gcggctggcc cggcagctcg gcgcgcccga tctcctccac
     5461 cagcccgcgg gcgagcagcg accgcaccgc gccgtcggcg tcgacgccgc ggatctgcga
     5521 cacctgcgcg cgggtgaccg gctgcttgta ggcgatgatc gccagggtct ccagggacgc
     5581 ctgcgacagg cggccggagc gcccgttcag cacgaagcgc tccacgtacg gagcggcgcc
     5641 cgggtcggtg tagtagcgcc agccgcccgc gacctcgcgc agcacgaagc cgcggccggc
     5701 gtcgacgtac tcgctgcgca gcgcgtgcag ggtggccacc acctgctcgg tgggggcctc
     5761 cagcacctgc gcgagcgtgt tcgcgtccac gggctcctcg accaccagca ggatggcctc
     5821 cagggccttg cgcagctcgt cgtccagcag caccgactgg gcggggacgg gggagttggc
     5881 gggatcggtc acggcttcgg cacctcggcg tcctcggggt cctctgcggc gcgggcgtcg
     5941 taggcctcca tggtcgcgaa cacggaaccg tgccacggct cgtcggcgcg cagctccacg
     6001 ccgatctcgc cgaagctggc cgcctgctcc agctccacgt gacccagctt gtacagctcc
     6061 agcagcgcca ggaagtgcac gatcacctcg atggggtgac ggcagccgcc ggtgagctcg
     6121 cggaacgtcg cgcgcccgcc ggcccggtcc agctcgtcca tgaccatgcc ggccgcctcg
     6181 cgcacggtca tgcggaccgg ctgcacgtgc gacaggtcca ccacgggctc cgggcgctcg
     6241 gtgagggcgc gcagcgccag ccgcgcgagg tcggcctgga cgagcgctac cgcaacctgc
     6301 tgccggacgt ggagctgacc atcacggccg aggagctcgc gcggcgcgta gccggtgaac
     6361 gcctccatgc gctcggagac ccagccggcg gcctccttga aggtgcggta gtccagcagg
     6421 cgcgcgtaca gcaggtcgcg cgcctccagc gccagctcct ccagctccgg gtcgtcctcg
     6481 gacggcagca ggcgcgccgc cttgagctcg atcagcgtcg cggcgacgac caggaactcg
     6541 gtggccacct ccaggtcgag ctcgtcgagc tggcggagga cggcgaggta gtcgtcggtg
     6601 atctgcgcga tcgggatgtc gtagatctcc accttccgcc tggcgatgag gtggaggagg
     6661 aggtcgaagg gaccctcgaa cgagcccacc gtgacgtgat aggtggtggc cattgcgcgc
     6721 cagtctaccg ggtgtgctcc tcctgaccga cgatggtgcg cagcagcgtc ggcggccgcg
     6781 gcacgtcccg gtcgaccagc cggacgatct ccgccaggcg cgccgccgcc atgtcggcgt
     6841 cgtcgcggcc gcgcgcgtgg acgaccgcca gcggctgccc cgcctccaca cggtcgccga
     6901 tctccacgcg cagctccacg cccaccgccg ggtcgacggc gtcctccttg cgcgcccgcc
     6961 ccgctcccag ctgcgaggac agctcgccga cggcgcgggc cggcacggcc tccaccaccc
     7021 cgccgcgcgg tgccggcgcc tccacgacga cgggggcgcg cggcagcacg gccccggggt
     7081 cgtcgcacac ggcggggtcg ccgccctggg cggcgatcat ggcgcgcagc cgttccagcg
     7141 cctggccgac gcgccacagc gcgcgcagct cgtcgcgggc gccgtccgcc ccggcgtcgc
     7201 cgcccgccga tccggacgcg cccgcccgga cccgcatgag ggcggcggag gccaggtcca
     7261 gggccagctc cgccagccgc ccgcgcggct cctcgcgcag cagccggacg cactcggcga
     7321 cctccagggc gttgccgatg ccggtgccca gcggctggtc catggcgctg accagcgcga
     7381 cggtgcgccg ccccgcggcc gtgccgatgc gcacgcacag ctccgccagc gccgcggccg
     7441 ccgcctcgtc cggcatgaag gcgccgtcac cggctttgac gtccagcacg atgtgcgcgg
     7501 cgccggaggc gagcttcttg gacatcacgc tggaggcgat cagcgccacg gacgggaccg
     7561 tggcggtgac gtcgcgcagc gcgtacagcg ccttgtcggc cggcaccagg cgctcggact
     7621 gcgcggccac cacgcagccg acctcctcgg cgatgcggcg cgcctcgtcc gggtccaggg
     7681 cggtgcgcag gccggggatg gcttccagct tgtccagcgt gccgccggtg tggcccaggc
     7741 cgcggcccga cagcttgacc agcgtgcagc cggcggccgc cagcagcggc gccaccagaa
     7801 gcgtggtgcc gtcgccgaca ccgccggtgg agtgcttgtc cacggtgggg cctggaacgc
     7861 cggacaggtc catgcggtcg ccgctgcgca gcagcacgtc ggtcagcgcg atcgcctcgt
     7921 cgtcgctgaa cccgcgcagc acgccggcca tgagcaaggc ggccatctgg ccctccatga
     7981 cggcgccgtc caggtagccg cgcacgagcc agtcaagctc cgcggcgctc agctcgccgc
     8041 cgtcgcgctt gcgcgccacc aggtccacgg cccgcagccg catggcaggt cctttctcac
     8101 ctcgcgcggg gatgcgcccg ctcgtacacc gagcgcaggc ggctcccgct gaccagcgtg
     8161 tacacctgcg tcgtgttgac gctggcgtgg ccgagcagct cctgcaccac acgcacgtcc
     8221 gcgcaccggt cgatcaggtg tgtcgcgaag gagtgccgca gcgtgtgcgg cgacacgcgc
     8281 tcgcccaggc cggcgtcctc cgcgtgccgc ttcacgatct tccagccgcc ctggcgggtc
     8341 agcctgccgc cgcggcggtt cacgaacagc gccgggccgg cggcccgcag ggccggccgc
     8401 ccgcgcacca gccaggcgtc cacggcggcc gccgcgacgc gcccgatcgg cacgatgcgc
     8461 tccttgcggc ccttgcccag gcagcgcacg gagcgggccg ccaggtcgac gtcgtccacg
     8521 tcgagctcca ccagctcggt gatgcgcagg ccggcggcgt acagcagctc gagcatggcg
     8581 cggtcgcgca gcgcgaccgg ctcgtcgccc accggcgagg ccagcaggcg ctcgatctcc
     8641 tccaggtgca gcgccttggg cagcgggcgt ttcggccggg ggccgctgag ctccgtggac
     8701 gggtcgaacc gcgccagccc ctcgcgcacc aggaaacggt gcagcccgcg caccgacacc
     8761 agcgtgcggg cgatcgtgga ggccgcgtac ggcttgccgg cggcggtcgt ggcgtcgcgc
     8821 agccaggcga cgaacccggc caggtcgccg tccgtggccg tcagcgggtc gtccgtgccg
     8881 atgcccgcca ggtagcgccc gtagacggcg aggtcacgct cgtaggccac caggctgtgc
     8941 cgcgacagtc ctcgttccac acgcaggtag tccaggtagc gcctggcgtg ctgcggcagt
     9001 gcgaccggga ccacctcctc ggacctcgtt ggcgtgccga gccaggctat cactttgttg
     9061 acataaccat tgttgtggac agcggagttg cgcgatctgg cctgggatct cagcctcatc
     9121 acatgtccat gccactcgca cggggagccg acgacatgta cgccgatgac gacgaccgtc
     9181 ccgccacgaa gaaggacctg cgcgtgctcg gggccgaact gcgcgcggag ttgcatcagg
     9241 tgcgcgcgga cctgctggac acgatcagca gccgcaccac aacgttgttc ttcagccaga
     9301 tcgcggctgt ggcggcggtc gcggcgatcg cgttcgcgga ctgatctcag tgcacgccgc
     9361 gctgcaggcg cgtcagcagg atcccgatca ccgtcttggc gtcccggatc tccccggcgg
     9421 ccaccatggc gaccgcctcg tccaggggca cccacacgac ctccatgtcg gcctcctcgt
     9481 gggcggcggt gaagccgtcg ggagtgcccg tctcgcgcag gtcgtcggcc aggtacaggg
     9541 tcgtgccctc gtcggtccac cccgccgagt tgtggaagga cagcaggggg tccagccgcc
     9601 cggcggacca gccggtctcc tcctccagct cccgccgcgc cgcgtccgcc gggtcctcgc
     9661 cctggcggtc gagcttgccg gcgggcagct ccagcaggcg ctcgcccacg gggtggcggt
     9721 actggcgcag cagcagcacc cggccctgcg cgtccagcgc caccacggcc acggcgtcgg
     9781 ggtgctcgac gatctcccgc tcggcctgcg agccgtcggc gaagcggacg gtgtccacgc
     9841 ggatgttcga cagctttccg gagaacgggc gctcgctggc ggtgacggtg acgtcgctca
     9901 tcgcgcgagc ctatcccccg ccgcgggcgc ggctacgcgc tgggcagcag cgcggcacgg
     9961 tggacgcggg cgtgccaggc gccggccagc gcggcgacca gcaccagcag cagcgcgggc
    10021 agtggcgcct cgctggccag ggccagcgcc accacgacgt tcatcgccac cacggccgtg
    10081 ctcaccacgc gcagcgtgcg cggcttgagg ccgcccgccc ccttggcgaa caggtccggg
    10141 tgccgggtgg gcagccgtgc cgccgccagg cacaccgcgg cgatggccac caccagcccg
    10201 aggttcatga gcacgccgag gaaggtcttg tccggtccgg ccagcagcga gagcaggctg
    10261 ccggcgaagc acaccgacag ggcgacgtgg ggcgtgccga agcggcggtt cacggtcccg
    10321 accgaacggg ggaacaaccc ctcctcggcc accacgagca tgccgcgcga caccagcgcg
    10381 aacacgccgt tgatggtggt cacgccggcc accacggcgc cgccgaggat gaacacgctg
    10441 acggcgcccg gcgacatgaa cgcctgcgcc acctccacca gggtggtgcc gtccatcgtc
    10501 tgccagggca ccgccccgac ggcgaccacg gtgatgccca cgtacagcgc caggacgatc
    10561 agcatgccca ggggcagcac ggccccgaag acgcggcccg gatcgacgac ttccgccccc
    10621 acctcgacca cgaacagccc gccggccagc agcgcgtaca gcagcacggt cgcggtgacc
    10681 aggcccatcg ccccgccggg cagcgggtcg gcgaagcgcg ccgcctcgac gtcgccccag
    10741 cccatcgcca cgaacagcac cagcgcgccg accagcacga acagcaggac gctctgcacg
    10801 gccatggcca ggcgcacgcc cagcaggttg aggacgaaga agaccacgag cacggcgacg
    10861 gccagcgtct cggccggcca gctcacccag gacaggcgcg ccaggtactc gccggtcatc
    10921 agcgcgaaca gcggctggcc gccgatcacg atggaaagca gcagcgccgc ggcggccatg
    10981 tacgcgccgc gcgggctcca cagcttcatg tagcggtagg acgcgccggc ggtcgggtag
    11041 gcgcgcgcga gcacgcggta gggcacgatc gcgagcagtg cggggatggc cgacagcgcc
    11101 atcgccagcg gcagcgacgg cccggtctcg gccgcggcga tgttgaccag cacgaacagc
    11161 ccgccgccga tgttgatgcc gacggacagc gcgagcaggc ccaggaagcc gatgtcgcgc
    11221 cggaggttgc cctcgccggc ccccatggcc tcgctcacgg cgcgggcgcg gtctccgtgg
    11281 cgtccacgac gatccgtgcc gacgcctcca cctcggctcg tgccgacggt tctggctcgg
    11341 ctcgtgccga tggttctgcc tcggctcgtg ccgatggttc gggcagccgg ccggcctggg
    11401 cgagcatgcg ctcgcgcgcc gcgccgacga agtcgcggaa cagtgggtgc gggcggttgg
    11461 gccgcgagcg gaactccggg tgcgcctggg tggcgacgaa ccacgggtgc ccggggatct
    11521 cgacgaactc caccaggcgg tcgtccgggg acagcccgct gaacgccatg ccggcctcgg
    11581 ccagccgcgg ccggtagcgg ttgttgacct cccagcggtg gcggtggcgc tcgtagacga
    11641 ccggctcgcc gtaggcggcg gccgccctgg tgccctggac cagcttggcc gggtacaggc
    11701 ccagccgcat ggtgccgccc atgtccgtga cgtcgcgctg gtcggccatg aggtccacca
    11761 cggggtccgg cgtgtgcggg tcgaactcgc tggagttggc ctcggccagg ccgagcacgt
    11821 ggcgcgcgaa ctccaccacg gcgacctgca ggccgaggca gatgccgagg tagggggtgt
    11881 cgcgttcgcg cgcgaagcgg gcggcgttga tcttgccgtc gatgccgcgc acgccgaagc
    11941 cgccgggcac caggacaccg tggacgccgc gcagcgcgcg ctcggccgcg tccgcgtcgc
    12001 tgaggtcgtc ggcggggacc cactcgatct cgacgtccac gccgtggtgt gccgggccga
    12061 ccgcccgctg tcgccggagc tcaagcgcaa gatcgcgctg ctctc
//
LOCUS       DATMKC010000287         7077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_74057, whole genome shotgun sequence.
ACCESSION   DATMKC010000287 DATMKC010000000
VERSION     DATMKC010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74057"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VNU01_08485"
     CDS             complement(<1..1145)
                     /locus_tag="VNU01_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32695.1"
                     /translation="MAVSVRARLEAQADGAVQVRAEHLHPVQGRQHVRVRVPVGVARA
                     GRHHGGVRADGRQEPLGVGGAAMVGHFQHLGAQPPRRAQRPLLRQHLAVAREQDAPAR
                     PVHPQQEAVLVRRRAGQAGARRAEHLHERPAQPERLAAVHAHDRDAEAAGGIQRPCGR
                     AAARHGRMRSDDQRGGPQPPQHLRHAAAVVEVAVAEHRDIEPLHPQAPQHRGGATGIR
                     PGVHQHPHGAPGTRRLDDDRVALPDVQRRERQGGHLRRQRPGQQPRHLGGQRHVDAQL
                     GPAGERGRHAAQRVQRRPGQRRQGRSPARVDLADRAGGQAGQLRRARQRHGQHVRGQR
                     EQRRLTQAPQQRRCHGQLRGHRGRDGGAHPARAAQPAGHRGAGGQDPA"
     gene            1144..2088
                     /gene="holA"
                     /locus_tag="VNU01_08490"
     CDS             1144..2088
                     /gene="holA"
                     /locus_tag="VNU01_08490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWH32696.1"
                     /translation="MPRPPAYLLAGPEELLLRRAADQVLAELREEAAGDLDVTEVRAS
                     ELGEHGLPDLRTASLFGGARAVVIREAQDLPAEASAALAAELEGTPPDAAIVLLATGT
                     GRIQKLAKRVKELGGRVDVAPPKDWEDAKWAALVAAEFQRHGRAAAKDAVQAILSHAG
                     LDVGGIAEKVAQVCATAPAGQVGAEHVEAVVVGHGNRGSFAVADAMCDRDAAEALRLL
                     RGVLDGGDDPVMVLGALAYRLRSIVAVAAGVDGKAVGLNISPGQARRLQGVRRNFGPG
                     ELTRAYRLLARADVEIKGGVLPPQLVIERAVAEIATRG"
     gene            2147..2764
                     /locus_tag="VNU01_08495"
     CDS             2147..2764
                     /locus_tag="VNU01_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH32697.1"
                     /translation="MGRREERRERTLAEIREAAIGCMADEGVAGLNLSEVARRVGMRT
                     PSLYEYFPSKLAVYDDLFGLGVRRCQAAVEEAVDGLAPGIGRLAAGNVAFVSWCAANP
                     VLAQLLFWRPVPGFEPSPASYAEARRMLERLREELADTARAGELSPDAARDEGLALYT
                     ALTQGVVSQHLANEPGVPFEKGRFTALLPVLLELFRHRYAPKEPR"
     gene            2761..3546
                     /locus_tag="VNU01_08500"
     CDS             2761..3546
                     /locus_tag="VNU01_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWH32698.1"
                     /translation="MTTTLTRAAELPPLDRDAGYRIARAELDASIAFYAALGAQEWDA
                     PTACAPWRVREMVAHMVGAMDESIRFRVLLRHVRQGRRRYPDKSDLDALNEAQVDERR
                     DWPPEALLRAFRDLGPRAVEARRKLPAPLRAVRIPKPDGGRMSLAYLNDVIYPRDLWM
                     HRLDAARATGRDFTLGEHDRALVAGVVRDLALGWTGPDVTLTLTGPAGGSWALGQRPG
                     PELRMDAVACCLLLSGREPGASPQAAPGAEAAVPVLRAARVLF"
     gene            complement(3614..3880)
                     /gene="rpsT"
                     /locus_tag="VNU01_08505"
     CDS             complement(3614..3880)
                     /gene="rpsT"
                     /locus_tag="VNU01_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWH32699.1"
                     /translation="MANIKSQIKRNRQNETARLRNKAVRSALKTQLKKFRTAAEGGDA
                     GAAQEAFQVAARQLDKAAQKGIVHGNFAANHKSKMAKKLSSLSS"
     gene            4025..5854
                     /gene="lepA"
                     /locus_tag="VNU01_08510"
     CDS             4025..5854
                     /gene="lepA"
                     /locus_tag="VNU01_08510"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWH32700.1"
                     /translation="MTYDLARIRNFAIIAHVDHGKSTLADRMLQATGLVSDRDMRAQY
                     LDKMDIERERGITIKAQAVRMPYKAADGSEYLLNLIDTPGHVDFSYEVSRAMNACEGA
                     ILLVDAAQGMQAQTIANLYVAIEAGLEIIPVMNKIDLPAARPEQVALEIASLLGGDPE
                     DILAVSAKTGQGVPEVLERVVERIPAPKGEPDRPPRALIFDSVFDPYRGVVVYFRVIE
                     GVFRPDMDIHLMATGFHNSIDELGVVSPEPKPVDALGPGEVGYLTAAIKEVGKAKVGD
                     TITDHGRPAAEPLPGYREPTPMVFSGLYPVDSDDFPELRDALEKLRLNDASFTFEPET
                     SVALGFGFRCGFLGLLHMEIISERLDREFDIPLVTTAPNVAYEVHLEEIAVKPNEEMV
                     LTVSNPAELPPPNKIDFILEPVVNAMLICPTEYVGAVMQLCEGRRGVMKAMDYMTEER
                     VELRYELPLAEIIFDFYDRLKSGTRGYASLDYEPGGMRRSKLAKVDILLNGEPVDAFS
                     SIVHVDKAYDYGKRMVNKLKELIPRQMFDVPIQAAIGSKIIARETVKARRKDVLAKCY
                     GGDITRKRKLLEKQKEGKKRMKNVGSVEIPQEAFVAALSVGGD"
     gene            5966..>7077
                     /locus_tag="VNU01_08515"
     CDS             5966..>7077
                     /locus_tag="VNU01_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH32701.1"
                     /translation="MCSSTGFEIDAALATADLHGLLDVAERALRRAKTLPLGGAVDDV
                     ALTQAVQRLEAVSRVAESHKCAVVGEIEQRHAARGQSAASTEDLLTRVLHLTPGEAKA
                     RTELAAGLKQLPDTAAAFADGRVGLSQAAVAVKKAAEVAGRDDAEVLLATIDRTASTV
                     GQTTNRTRLAREIDTAVAKTGVDVLAERERRAFQDRKLQWSVRDGMDVLTAHLDPIGA
                     ATVRGMLDALSDKTSERDSRTFVQRQADALVDLATLAHTARNIAKTTMPPSKVLLITT
                     PDALHGLPGAEPATIDGHGPVSPELARQLCCDSAVTPVVTGRNGEVLDVGREERLPTT
                     KQRNAVIARDKTCVGCDAPVSRCQIHHIKWWGKGGAT"
BASE COUNT          951 a         2520 c         2655 g          951 t
ORIGIN      
        1 gcgggatcct ggccgccggc cccgcggtgg cccgcaggct gcgcggcccg cgccgggtgc
       61 gcgccgccgt cgcggccgcg gtggccgcgc agctggccgt gacaccgccg ctgctgtggc
      121 gcctgggtga ggcgccgctg ctcgcgctgc ccgcgaacgt gctggccgtg ccgctggcgg
      181 gcgcggcgca gctggccggc ctggccgccg gcgcggtcgg ccaggtcgac gcgcgcgggg
      241 gagcggccct ggcgtcgctg gccgggccgc cgctgcacgc gctgcgcggc gtggcggccg
      301 cgttcgccgg cgggcccaag ctgcgcgtcg acgtgacgtt gcccgccgag gtggcgcggc
      361 tgctgcccgg ggcgctgccg gcgcaggtgc ccgccctggc gctcacggcg ctggacgtcg
      421 ggcagggcga cgcggtcgtc gtcgaggcgc cgggtccccg gcgcgccgtg cggatgctgg
      481 tggacgccgg gccggatccc cgtcgcgccg ccgcgatgct gcggcgcctg ggggtggagc
      541 ggctcgatgt cgcggtgctc agccacggcg acctcgacca cagcggcggc atgccggagg
      601 tgctgcggcg gctgcggccc gccgcgctgg tcgtcggacc gcatccgccc gtggcgcgcg
      661 gcagcccggc cgcacggtcg ctggatgccg cccgccgcct cggcatcccg gtcgtgcgcg
      721 tgcacggcgg ccagtcgttc cggctgggcg gggcgctcgt ggaggtgctc ggcccgccgc
      781 gcgccggcct gcccggcgcg ccgccgaacg agcacagcct cctgctgcgg gtgcacgggc
      841 cgggcgggcg catcctgctc acgggcgaca gcgaggtgct ggcgcaggag tgggcgctgc
      901 gcacgccgtg gcggctgcgc gccgaggtgc tgaaagtgcc ccaccatggc ggcgccacca
      961 acgccgaggg gttcctggcg gccgtcggcg cgcacaccgc cgtggtgtcg gccggcgcgg
     1021 gcaaccccta cgggcacccg cacccggacg tgctggcggc cctggacggg gtgaaggtgc
     1081 tccgcacgga cctgcacggc accgtccgcc tgcgcctcca ggcgtgctcg tacactcacc
     1141 gccatgccgc gaccgcccgc ctacctgctc gccggacccg aggagctgct gctgcgccgg
     1201 gccgccgacc aggtgctcgc cgagctgcgc gaggaggcgg ccggcgacct ggacgtcacg
     1261 gaggtgcgcg cgtcggagct gggcgagcac ggcctgcccg acctgcgcac cgcctcgctg
     1321 ttcggcggcg cgcgcgccgt ggtgatccgc gaggcgcagg acctgccggc cgaggcgagc
     1381 gccgcgctcg ccgcggagct ggagggcacg ccgccggacg ccgccatcgt gctgctcgcc
     1441 acgggcaccg ggcgcatcca gaagctcgcc aagcgcgtca aggagctggg cggccgcgtc
     1501 gatgtcgcgc cgcccaagga ctgggaggac gcgaagtggg ccgcgctggt cgcggccgag
     1561 ttccagcgcc acggccgcgc cgccgccaag gacgcggtgc aggcgatcct gtcgcacgcc
     1621 gggctggacg tcggcggcat cgccgagaag gtcgcgcagg tgtgcgccac cgcgcccgcg
     1681 ggccaggtcg gcgccgagca cgtcgaggcg gtggtggtcg gccacggcaa ccgcggctcc
     1741 ttcgccgtcg ccgacgcgat gtgcgaccgg gacgccgccg aggcgctgcg cctgctgcgc
     1801 ggtgtgctgg acggcggcga cgacccggtc atggtgctgg gcgccctggc ctaccggctg
     1861 cgctccatcg tcgcggtggc cgccggggtg gacggcaagg cggtggggct gaacatctcg
     1921 cccggccagg cgcggcgcct gcagggtgtg cgccgcaact tcgggccggg cgagctgacg
     1981 cgggcctacc gcctgctggc ccgggccgac gtcgagatca agggcggcgt gctgccgccg
     2041 cagctcgtca tcgagcgggc ggtcgcggag atcgccacgc gcggctgagc ccttgaccca
     2101 ctgagcctaa tgtcgttagg gtttcctagc ggtgctagga ggctgcatgg gcaggcggga
     2161 ggagcggcgg gagcgcacgc tcgcggagat ccgcgaggcg gcgatcggct gcatggcgga
     2221 cgagggcgtc gccgggctca acctcagcga ggtggcgcgg cgggtgggca tgcgcacgcc
     2281 gtccctctac gagtacttcc cgtccaagct ggccgtctac gacgacctgt tcggcctggg
     2341 cgtgcgccgc tgccaggccg ccgtcgaaga ggccgtcgac ggcctggctc ccgggatcgg
     2401 ccgcctggcc gcgggcaacg tggcgttcgt gtcctggtgc gcggccaacc cggtgctggc
     2461 ccagctgctg ttctggcggc ccgtcccggg cttcgagccc tcgcccgcct cctacgccga
     2521 ggcgcggcgc atgctggagc gcctgcgcga ggagctcgcg gacaccgccc gggccggcga
     2581 gctgtcgccg gacgccgcgc gcgacgaggg gctggcgctg tacaccgccc tgacgcaggg
     2641 cgtggtgtcc cagcatctgg ccaacgagcc cggcgtgccg ttcgagaagg ggcgcttcac
     2701 cgccctgctg cccgtcctgc tggagctgtt ccgtcaccgc tacgccccga aggagccccg
     2761 atgaccacca cgctgacgcg cgcggcggag ctgccgccgc tggaccgcga cgccgggtac
     2821 cgcatcgccc gcgccgagct ggacgcgagc atcgccttct acgccgcgct cggcgcgcag
     2881 gagtgggacg cgcccacggc gtgcgcgcca tggcgcgtcc gcgagatggt ggcgcacatg
     2941 gtcggcgcga tggacgagtc gatccgcttc cgcgtgctgc tgcgccacgt gcggcagggg
     3001 cgcaggcgct acccggacaa gtcggacctc gacgcgttga acgaggcgca ggtcgacgag
     3061 cggcgcgact ggccgcccga ggcgctgctg cgggcgttcc gcgaccttgg cccgcgcgcc
     3121 gtcgaggcgc ggcgcaagct cccggcgccg ctgcgcgccg tccgcatccc caagcccgac
     3181 ggcgggcgca tgagcctggc ctacctcaac gacgtcatct acccgcgcga cctgtggatg
     3241 caccggctcg acgcggcgcg cgccacggga cgcgacttca ccctgggcga gcacgaccgc
     3301 gcgctggtgg ccggcgtggt gcgcgacctg gccctgggct ggaccgggcc ggacgtcacg
     3361 ctgacgctca ccgggccggc cggcggaagc tgggcgctgg gacagcgtcc cgggccggag
     3421 ctgcgcatgg acgcggtcgc ctgctgcctg ctgctgtcgg gtcgcgagcc gggcgcgtcg
     3481 ccgcaggcgg cgcccggcgc ggaggcggcg gtgccggtcc tgcgcgccgc ccgggtgctg
     3541 ttctgaccct gctggcccgc ggaacgcgaa acggcgcccc gaggggcgcc gcatgcgaag
     3601 cgaccgaagc gtcctaggag gacagcgagg acagcttctt ggccatcttg gacttgtggt
     3661 tggccgcgaa gttgccgtgc acgatgccct tctgcgcggc cttgtcgagc tggcgcgccg
     3721 caacctggaa ggcctcctgc gccgccccgg cgtcgccacc ctcggccgcc gtgcggaact
     3781 tcttcagctg cgtcttcagc gcactgcgca ccgccttgtt gcgcaggcgc gccgtctcgt
     3841 tctggcggtt gcgcttgatc tggctcttga tgttggccat gcgggcttca gttccttgga
     3901 cgctggtcgg ggaggctgct cggtctgggc gcgcgcggag agatccggcg cacggacgtg
     3961 acggcgagtg tagcgggggg ctgtggagcc gtccacctgg cggcccggct accatcggcc
     4021 ccacatgacg tacgacctcg cgcgcatccg caacttcgcc atcatcgccc acgtcgacca
     4081 cggcaagtcg acgctggccg accgtatgct gcaggccacc ggcctggtca gcgaccgtga
     4141 catgcgcgcg cagtacctcg acaagatgga catcgagcgc gagcgcggca tcaccatcaa
     4201 ggcgcaggcg gtgcgcatgc cgtacaaggc cgccgacggc agcgagtacc tgctgaacct
     4261 catcgacacg ccgggccacg tcgacttcag ctacgaggtc agccgcgcga tgaacgcctg
     4321 cgaaggcgcg atcctgctgg tggacgcggc gcagggcatg caggcccaga ccatcgcgaa
     4381 cctgtacgtc gcgatcgagg ccggcctgga gatcatcccg gtcatgaaca agatcgacct
     4441 gccggccgcg cggccggagc aggtcgccct ggagatcgcg agcctgctgg gcggcgaccc
     4501 cgaggacatc ctcgcggtgt cggccaagac cgggcagggc gtgcccgagg tgctggagcg
     4561 ggtggtggag cgcatccccg cgccgaaggg cgagccggac cggccgccgc gcgcgctcat
     4621 cttcgactcc gtcttcgacc cctaccgcgg cgtcgtcgtc tacttccgtg tcatcgaggg
     4681 cgtgttccgg ccggacatgg acatccacct catggcgacc ggcttccaca actccatcga
     4741 cgagctcggc gtggtgtccc cggagcccaa gccggtcgac gcgctgggac cgggggaggt
     4801 gggctacctc accgcggcca tcaaggaggt cggcaaggcc aaggtcggcg acaccatcac
     4861 cgaccacggc cgtcccgcgg ccgagccgct gcccggctac cgcgagccca ccccgatggt
     4921 gttcagcggc ctgtacccgg tggacagcga cgacttcccg gagctgcgcg acgccctgga
     4981 gaagctgcgg ctcaacgacg ccagcttcac gttcgaaccg gagacgtccg tggcgctggg
     5041 cttcggcttc cgctgcggct tcctggggct gctccacatg gagatcatct ccgagcggct
     5101 ggaccgcgag ttcgacatcc cgctggtcac cacggcgccc aacgtggcct acgaggtgca
     5161 cctggaggag atcgccgtca agccgaacga ggagatggtc ctcacggtgt ccaacccggc
     5221 ggagctgccg ccgcccaaca agatcgactt catcctggag ccggtcgtga acgccatgtt
     5281 gatctgcccc acggagtacg tgggcgcggt gatgcagctg tgcgagggcc gccgcggcgt
     5341 gatgaaggcc atggactaca tgaccgagga gcgcgtggag ctgcgctacg agctgccgct
     5401 ggcggagatc atcttcgact tctacgaccg gctgaagtca ggaacgcgcg gttatgcctc
     5461 gcttgactac gagccgggcg gcatgcgccg cagcaagctc gccaaggtcg acatcctgct
     5521 caacggcgag ccggtggacg cgttcagctc catcgtccac gtcgacaagg cctacgacta
     5581 cggcaagcgc atggtcaaca agctcaagga gctcattccc cggcagatgt tcgacgtgcc
     5641 gatccaggcg gcgatcggct ccaagatcat cgcccgcgag accgtgaagg cccgccgcaa
     5701 ggacgtgctg gccaagtgct acggcggcga catcacgcgc aagcgcaagc tcttggagaa
     5761 gcagaaggag ggcaagaagc gcatgaagaa cgtggggtcg gtggagatcc cgcaggaggc
     5821 cttcgtcgcc gcgctctccg tggggggcga ctgaccctgg cgtcctcgcg gcgttgcggg
     5881 gccgccccac gctggagacg actcgtttcc cacagattca atttcgtggg ttgacctgtc
     5941 gcagtcgcgt tgtatggtat cgaacatgtg ttcgagtacg gggttcgaga tcgacgcggc
     6001 gctggccacc gcggaccttc acggtctgct ggacgtggcc gagcgcgcgt tgcgccgcgc
     6061 caagacgctg ccgctgggcg gcgccgtcga cgatgtggcg ttgacccagg ccgtgcagcg
     6121 cctggaagct gtgagcaggg tcgcggagtc gcacaagtgc gcggtcgtgg gcgagatcga
     6181 gcagcgccac gccgcccgcg gccagtccgc cgcgtccacc gaggacctgc tgacgcgcgt
     6241 gctgcacctc acccctggtg aggccaaggc gcgcaccgaa ctcgcggcag ggctgaagca
     6301 gttgccggac acggctgcgg cgttcgcgga cggccgcgtg ggcctgtccc aggccgcggt
     6361 ggccgtcaag aaggccgccg aggtcgccgg ccgtgacgac gccgaggtgc tgctcgccac
     6421 gattgaccgg acggcgtcga cggtggggca gacgacgaac cggacgaggt tggcccgtga
     6481 gatcgacacc gcggtcgcga aaaccggtgt ggatgtgttg gcggagcggg aacgccgcgc
     6541 gtttcaggac cgcaagctcc aatggtccgt ccgtgacggc atggacgtgc tcaccgcaca
     6601 cctggacccc attggggcgg ccacggtgcg ggggatgctg gacgccctgt cggacaagac
     6661 gtcggagcgt gacagccgga cgttcgtgca acgccaggct gacgcgctgg tggacctggc
     6721 gacgctggcg cacaccgccc gcaacatcgc caagaccacg atgccgccgt ccaaagtgct
     6781 gctgatcacc acccccgacg ccctgcacgg tctcccaggc gcggagcccg cgacgatcga
     6841 cggtcacgga cccgtctcac ccgagctcgc gcggcagctg tgctgcgact cggcggtgac
     6901 gccggtggtg accggccgca acggcgaggt cctggacgtg ggtcgtgaag agcggctgcc
     6961 gacgaccaag cagcgcaacg cggtgatcgc ccgcgacaag acctgcgtcg gctgcgacgc
     7021 gcccgtgtcc agatgccaga tccaccacat caagtggtgg ggcaaaggcg gcgccac
//
LOCUS       DATMKC010000288        28265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_166150, whole genome shotgun sequence.
ACCESSION   DATMKC010000288 DATMKC010000000
VERSION     DATMKC010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 28265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28265
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166150"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1061)
                     /gene="rsmH"
                     /locus_tag="VNU01_08520"
     CDS             complement(45..1061)
                     /gene="rsmH"
                     /locus_tag="VNU01_08520"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWH32702.1"
                     /translation="MDPTPDVPAGGAPHLPVMAARVVELLLDGLRPGPALIVDATIGA
                     AGHAAALLAARADLHLVGFDRDPDALALAERRLAAYAGRVTLVHSGYDQINHHVTPVV
                     EHADVPPVGVLYDLGVSSMQLDQAQRGFSFRAEAPLDMRMDPTSDVSAANLVNELDVD
                     ELVDLIRRYGEERQALRIARAIVAARPLRTTTQLADVVYGAVPAAVRRNSAVHPATRT
                     FQALRIAVNRELERFEASLPQASDLLAQGGRIAVLSYHSLEDRITKRFFSDAATGCIC
                     PPDLPVCGCGRVPLLRPVTRGAEQPDGDEVAANPRARSAKLRAAERVRANDAFPLVRE
                     SEHA"
     gene            complement(1100..1255)
                     /locus_tag="VNU01_08525"
     CDS             complement(1100..1255)
                     /locus_tag="VNU01_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32703.1"
                     /translation="MDVLTATVDHPADLNGDLGRELVASALLMLLVLTSTGVVLGSVL
                     GALWLFA"
     gene            complement(1255..1689)
                     /gene="mraZ"
                     /locus_tag="VNU01_08530"
     CDS             complement(1255..1689)
                     /gene="mraZ"
                     /locus_tag="VNU01_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWH32704.1"
                     /translation="MFLGEYQHTLDPKGRVILPSAFRDRLKDGLVITVGLDNCLTIHP
                     VDDWQRVLASLRNLRTTDRRERMFARVMTSSAHSEELDKQGRVTIPARLREYAALAKD
                     VTVVGADMRVELWDAGRWETYRDSAMADFANTEQPFDLGGIF"
     gene            complement(2135..2812)
                     /locus_tag="VNU01_08535"
     CDS             complement(2135..2812)
                     /locus_tag="VNU01_08535"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWH32705.1"
                     /translation="MDAGPPVLRMRDDSAAVMADWSAGHPHREPPAVAAGGLGGLASG
                     HAHRAERQRRPSPHHKALVLNATMEPLCVVPARRALVLVLNDKADVVQTNGHRYRSER
                     LEYAAPSVVRLRRFVHVPYRRRAALSRRGVFIRDNFTCQYCGRAAENVDHVVPRSRGG
                     PHEWENVVAACRRCNSKKKDLMPAECGMVLRRAPFAPRAAFWLVVAVGGFHDDWQPYL
                     GDSLAAL"
     gene            3028..4011
                     /locus_tag="VNU01_08540"
     CDS             3028..4011
                     /locus_tag="VNU01_08540"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH32706.1"
                     /translation="MAARRTVGRRAAVDATQLAELFAQIEGNVTTVVEGKPEVVRLAL
                     VALIAEGHVLVEDVPGVGKTLLAKAIARSIACSVRRVQFTPDLLPSDITGVTIYNSEQ
                     GDWEFKPGALFANIVLGDEINRAGPKTQSALLEAMEEHTVTVDGTTHELGVPFMVIAT
                     QNPIELEGTYPLPEAQRDRFLMRLEIGYPSLDAELEILDVHGKSDRLEGMTPVADTRQ
                     VAAMIAACREVHAADPLKRYIVELVRSSRRHPAVELGASPRASLALLRAARALAAASG
                     RDYVVPDDVKSLARPVLAHRLILAPDARLSRGGAEEVVDELLDSIPIPVQR"
     gene            4025..5281
                     /locus_tag="VNU01_08545"
     CDS             4025..5281
                     /locus_tag="VNU01_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWH32707.1"
                     /translation="MVTERGAGLLIGAAFTWLLGRFLGVPELYVVAGAGCALAGLSVA
                     SVWLGSARLDAGRTVSPARIAWGGRTVVRVALRNTGRLPSGLLLVSDEIPYALADAPR
                     FSVPGLARRAQVDLGYELHGAGRGRYDVGPARVRLRDPFGLAERVRRVGATAELVVYP
                     RVERLGGGVPAAGRQGDGGAHRRTLLHAGDDFSTIREWAPGDDVRLVHWPSSAHRDRL
                     MVRQFEQPYEPLATLVCDTRALVHRGAGPASTLETAVTAVASVAGHLAAHGHRLRLAL
                     PEDRETPPIQPLERLMDRLAMVEASRETSLAPALGGLAGRASGGLLVAALAPPADGGE
                     RLGAHPDIRALLAAGRPYSVRIALVVDTGALAGPRGGQERPAALLAAALHGAGWRTAL
                     LRPGAPMSDAWNRVAGPTAMVLGGAR"
     gene            5278..7647
                     /locus_tag="VNU01_08550"
     CDS             5278..7647
                     /locus_tag="VNU01_08550"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWH32708.1"
                     /translation="MTAVAPQRPPVHAGATPAPAGPVTDPAARGPATPRPPLPGVAAA
                     VACVALLLATVLPLDRVYADRSWVAPTVSAALLAFGVGWLARRLRLGPVLSVSCNVAA
                     WALFSGLAFLPDTLLAGLVPTPSTVAAGVAAWAHGIELLQLRPAPAVPETGLVLITAT
                     GIWAVAHAVHELVFRAHAPMRAVLMAMVMWIVPLGLSPARGEAWLWAVPFLGAAALLL
                     LAAGAGEAARWGRWSPPAQGRRRLGLPVGARNPLGWLLAAAAIAAGVMGAPLLPGWTD
                     PALYELRDLGGSTTTTNPIVSIRASLLSPDPRPVLQVRTPRPVYLRTTSLDRYSDHEE
                     WTNDGIRGTPFRAGSVVPFEVPIRTALDVPVEVSVAALDAAVLVPMPYQPRVVDGTIG
                     DVLQYDQRLSTFTLNKGVRLSQGDVYAVSAAVPAPLAEQLDRVPAGWYAGELTRLPPS
                     VPAEVAQLARDITGRAGAVTPFQQAMAIQEELRAWTYSLDPPSGHSGEAMRTFLRTRT
                     GYCEQFAGTMAVMLRTLGIPARVGVGFTPGELADAAAGEYLIRSSNAHAWVEVLFPDE
                     GWIAFEPTPRTDGNVLVPSPANLAPSATQAQQAGDIPDAPEADDLPRQGPERLPTSAP
                     SPAATSPSAPGAGGGDGGGTTLLAAALLLLAFAGVGGTVLRRTRVDVDALGPAAQVLH
                     AVAKVERVSAAMGRPRKDTETDREFLGRITRSASGPALAAQAERARYATELPLESITL
                     ARTVAADLVQRLLYPLDRRSRATVLAQVRLAELRERAARALPGRFRRRK"
     gene            7730..8071
                     /locus_tag="VNU01_08555"
     CDS             7730..8071
                     /locus_tag="VNU01_08555"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HWH32709.1"
                     /translation="MPLSDREQQILADIEARLRAEDPKFARAVQTTTVSAHLRRRIKL
                     SAAAFVVGFLSLLLLVLHIGFGMAGFALMLAGAVSGINALKRLGQAEGPLGDQIRGGF
                     ERYRSDRDREE"
     gene            complement(8105..8755)
                     /locus_tag="VNU01_08560"
     CDS             complement(8105..8755)
                     /locus_tag="VNU01_08560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH32710.1"
                     /translation="MGELAVTRLDRASAKPVMAQVAGLSARAFDDYPFMRELFPGEAG
                     DPRRAGIARRFYAATVADCLAHGVVEVALDGDRLAGYCAWLRPGAYPMPAGRTVAFLP
                     AAATALRRYPRRARLVAQALARVERPHPDHPEHWYLAAIAVDPADQGRGVGARLIAPG
                     LRRADDGGEPCFLETTRPTAKDWYTRLGFAVQHAEPAFEGGPLQWFQWRDPQRRRR"
     gene            complement(8764..9582)
                     /locus_tag="VNU01_08565"
     CDS             complement(8764..9582)
                     /locus_tag="VNU01_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWH32711.1"
                     /translation="MSDRVMFEVGDDKVGVVTLNRPDKLNALDRGVFEGLHDAAAQAR
                     AAAEDGAVKAVLLRGAGRAFSSGLDVSLFGEMAAGDPSLQAAGGGTQDGMIAWLQQAF
                     TAWEDLPVPTVAAVRGVALGGGCQLAAACHLRVAAPDASIGVLEVKWAIIPDLGGTYR
                     LPRLVGLSRAIDMAVSARRVDAATALAWGLVDAVLDDEDFDAAARSYAAALAAGPTLA
                     QAGAARLMRESLDRTREDALAAERATQAGCLSSADFGEAVAAAMQRRAPDFAGR"
     gene            complement(9613..10866)
                     /locus_tag="VNU01_08570"
     CDS             complement(9613..10866)
                     /locus_tag="VNU01_08570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWH32712.1"
                     /translation="MSATLARRTYARRVAVAEPILHVDMDAFYASVEARKDPALLGRP
                     VLVGGTGGRGVVASASYEARAAGVRSAMPMARALRLCPDAVVLPPDFDAYQRVSGRIR
                     EIFLAVTPLVEPLSLDEAFLDVGGSVRLLGPPPRIAELVRGRIRDELGLPSSVGIAPN
                     KYLAKLCSGKAKPDGILHLRAEEVGAFLAPLPVRDLWGVGEQTAAKLDRFAIRTLADV
                     RATPEATLARLLGPAAAHQIRTLADGRDDRPVVPHEPAKGMSAEETFAHDVDDLAVLR
                     RELLRLSEKVARRLRKGNVAGRTVTLKLRYASFETVTRSRTLLMPTAEATELHREALA
                     LLEGLRLERVRVRLIGVGVTNLVPEDASRQLSLTADDRWSRLERAADVARDRFGGEAV
                     TRGALLRDAAPTSGDESARREDWQH"
     gene            complement(10863..13838)
                     /gene="dnaE"
                     /locus_tag="VNU01_08575"
     CDS             complement(10863..13838)
                     /gene="dnaE"
                     /locus_tag="VNU01_08575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWH32713.1"
                     /translation="MDVYGPSSVLVGVTHHLAPGDDARARARFALADRLGLRAVANQE
                     PRYLDAAHARIADVLDAVRLQAPLRTVARGRANDQGYLKTPEEMAEVFRERPDAVANA
                     AWVAERCEAELELGRLRVPDFVALGQDALAELKARAEAGIRERYASPAERHWDTFERE
                     LAMCARMGLAPYFLTVAEIVARIRSKGIIAACRGSAAGSILIYALRISDVDPIAHDLA
                     FERFMNPYREELPDIDIDVESARREDIYRDILDRYGDDRVACVAMVETFQARMAIREA
                     GKAIGLPQAEIDVIAKAFPHLRARDVRKGLDHLPELRGRNLDRPDLAFLFDAVEALDG
                     LPRHIALHPSGVLLADATLRDLVPMEHSAHGFAMAQFDKDDVEALGLCKLDVLAVRML
                     SAMSHCVDEVRRVRGQDVALSAIPWHDEDTYELIRSTRTLGMFQIESPGQRDLIGRLQ
                     PAELHDLVVDISLFRPGPVKGDMVRPFVARRHGREPTVYPHPILERALAETHGVIVFH
                     EQVMRCVAAVTGCDLAVADLVRRRLSDAERLEEIRDWVLAGCAERGIDEATARRLWHA
                     LEQFASFGFCKAHAAAFAVPTWRSAWLKRHYLPEFVAGLLTHDPGMYPRRLLLEDARR
                     LGVAVLPLDVNRSARDYRVERVPADHAGMAGCATGPAGGAREPGVPVGPSHSPGASGN
                     PLYVGAGRWAGPTPSLPAAPASAVPDLGSDPAEPSVRFGIRLALCDVAGIDERMIESV
                     LAHRPFTSVADLRDRSALSAPVAEALAHVGALECLAPGRSRRDIVLEVAERWSGARTK
                     AAAAKPVQGELLAEAAAGLADYTLSERVRAELEVLGLDASAHVVSFYEPLLEALGVTR
                     AAGLLDRPDGALVRVAGVKVATQTPPIRSGQRVVFLSLDDATGVADLAFFERVLERCA
                     HTVFHSWLLVVEGRVRHGGGRAVSVNAHSAWDLRDLLDAWRGDRLRAVLRQGRIAGMP
                     PRIGERQTPLWHRSGGSAG"
     gene            complement(13799..14365)
                     /locus_tag="VNU01_08580"
     CDS             complement(13799..14365)
                     /locus_tag="VNU01_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWH32714.1"
                     /translation="MFAHLAARTCYSMRHGALRPGELAQAVAERGMRAVGVADRDGLY
                     GAVRVAQACQAAGVRPIYGADLALRPDDARPGWAARVQRGRFAGPGSGPAWLDDGPSR
                     VTLIARTTPGYANLARTVSDAHLESVRGTPRLTWAQMERRPDAQFVLLGDDSPVGRLL
                     DAGRPDAAAASGRAAGWTCTGRAPCSSA"
     gene            14520..15284
                     /locus_tag="VNU01_08585"
     CDS             14520..15284
                     /locus_tag="VNU01_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWH32715.1"
                     /translation="MEPVYTPVIGAAKTLIAAMNWKIQIHGAEHIPASGPALLASNHI
                     GYLDFVFIGYGALDRGRMVRFAAKKEVFDHKVSGPLMRGMRHIQVDRFGGADAAVVAS
                     VAAMRRGEVIGMFPEGTISRSFIPAAGKTGAARMAMDAQAPLIPTAVWGTQRILTKGR
                     PKNFQRGVVIDVRYGEPVACQPGDDPTEVTKRLMDAITGLVENAAREYPQRPAGDGDR
                     WWLPASMGGTAPTVEEAEAMAAAERAERRAKRQREG"
     gene            15341..15874
                     /locus_tag="VNU01_08590"
     CDS             15341..15874
                     /locus_tag="VNU01_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32716.1"
                     /translation="MRIALLTVTLALAAACAGPDGAEVSFSTPPDDARTQAVPPEPAA
                     TREAATPGAATASAVPAQTPGPAAPRTVPASPTPSGGPRELGADEDAKVGQPYLYRLY
                     THCGIEATWWNGAWWDADPPLSDGSGNPPPGWGNPYQDGVIVQVSETRLEFTGDEGQR
                     AAFRPRPADVGAPPPCD"
     gene            complement(15950..18694)
                     /gene="aceE"
                     /locus_tag="VNU01_08595"
     CDS             complement(15950..18694)
                     /gene="aceE"
                     /locus_tag="VNU01_08595"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWH32717.1"
                     /translation="MPTQLPDVDPQETREWIESLDAVVQREGRQRARYLLLRVLERAR
                     EQQVGVPHLRSTDYINTIPPDREPPFPGDEYIERRIRAYIRWNAAVMVSRANRPDIGV
                     GGHIATYASSASLYEVGFNHFFRGKGTSDDPRHGDQIFFQGHASPGIYARAYLEGRLS
                     EEHLDLFRQETRRTSDGRAGLPSYPHPRLMPHFWEFPTVSMGLGPINSIYQARFNRYL
                     HHRGIADTGDSRVWAFVGDGEMDEPESQGALTVAAREELDNLIWVVNCNLQRLDGPVR
                     GNGKIIQEMESLFRGAGWNVIKVVWGRKWDELLALDTTGALVSKMNATPDGQFQTYSV
                     SDAAYIREHFFDSPELRRILEQSSLTEADLPVLDRGGHDYGKVYAAYEAAVNHAGQPT
                     VILAQTIKGWTLGPDFEARNATHQMKKLTQKALKKFRDRLYLDIPDEALESDLPPYYH
                     PGEDSDEIQYMLERRLELGGPLPARRVRVHPVSLPERDAYAELKQGSGKHDVATTMAF
                     VRLLKDLLKHPDLGKRIVPIIPDEARTFGMDAMFPTEKIYSPHGQTYEPVDREMLLAY
                     RESTQGQILHEGITEAGSMASFHAAGSAYATFSEHMLPVYVFYSMFGFQRTADLIWSA
                     ADQRCKGFLIGATAGRTTLNGEGLQHEDGHSLLYAATNPAVVSYDPSFAYEIAVVVED
                     GINRMLGNQADPDGPASSSEAVGHGADLMYYLTVYNEPVPQPPMPDGLDERLVLRGLY
                     RYLEAPDGDGPLARVLTSGSAMPAALRARDLLAEDWGVRTEVWSAPGWVQLHREGVAC
                     DEWNLLHPEEEPRVPWVTEALRGAGDAPVIAVTDYQKAVPLLVAPWVPAPYHVLGTDG
                     FGRSDLRSALRRLFRIDAEHVALAALHELAKQGAVEAKLVREAVERYGIDPDATTELP
                     "
     gene            18850..19335
                     /locus_tag="VNU01_08600"
     CDS             18850..19335
                     /locus_tag="VNU01_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HWH32718.1"
                     /translation="MTKDPGEAMPVHGGRTWVLAAVLCVALAACGGGAEPGSETSVFR
                     IRPGDCFNDPPAERQLDQVDVVRCDQPHDNEVFAAIEHPAAPEEPFPGRDALVGYAEQ
                     GCPEPFADYVGATYDQSRFSLFPIVPSADTWAKGDRQIICALYDHRAGKLTGSVRGSG
                     Q"
     gene            complement(19324..19944)
                     /locus_tag="VNU01_08605"
     CDS             complement(19324..19944)
                     /locus_tag="VNU01_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HWH32719.1"
                     /translation="MSATAAALVPERPTLPKLREAAAGCQACELWRTGTQTVFGEGPR
                     RARLLLVGEQPGDREDREGHPFVGPAGRLLDEALEEAGIARADVYLTNIVKHFRWKAQ
                     AGKGGKRIHAKPNAEHVEACRPWLEAELAVVRPELVVPLGATAAQGLLGRSFRVTRER
                     GQVHERDGVRYVATVHPSSILRAGERRAAQRADFVDDLRRVRALLA"
     gene            complement(19941..20627)
                     /locus_tag="VNU01_08610"
     CDS             complement(19941..20627)
                     /locus_tag="VNU01_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HWH32720.1"
                     /translation="MTFSIVARCARTGQLGVGAVTGTPGVGQLVTWAEAGVGAVATQA
                     WVNPYLGVDGLDLMGNGHPAERTLRAVVAMDDDRRLRQVGMVDAHGTSAAYTGERCER
                     WAGHRSEQDVAVQGNLLEGPETLDACLEAYAGSPGSDLVDRLIGALIAGEKAGGDRRG
                     ARSASVLVVATERYPLWDLRIDDSDRPLEALTDLRDDIAETLLPQILRLPTRQDSQGG
                     LHADDHAGLV"
     gene            complement(20651..20902)
                     /locus_tag="VNU01_08615"
     CDS             complement(20651..20902)
                     /locus_tag="VNU01_08615"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HWH32721.1"
                     /translation="MSKRYREPVEVQTTERGAPSAFRWRGAAYRVRGVLGHWREDAGW
                     WEGGGMAVPQRDLWRVEVGAGVYELVREGGAWRLDRVWD"
     gene            complement(20985..21884)
                     /locus_tag="VNU01_08620"
     CDS             complement(20985..21884)
                     /locus_tag="VNU01_08620"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWH32722.1"
                     /translation="MGRRRVLAASLALCAAVAGCRGGGEDAVVTLGVGSTAEQRMLAA
                     LTVVALDRAGIRVEPRPDLGGTVGLRREGLRGNVELWWDYTGAAWALGMGEQAPPADT
                     DESYQRVRRADEDRGLVWLEPTAANATLALYVRGDALPPEGSPRGMSWLAGVLSGGEA
                     ALCADRDFVRRDGGLDALAEAYAIGMDRLRVVAADEATAIRRVAAGSCFAGLATATSG
                     EARAAGLVPVADELAIFPAFVVAPVARAAALARHPGLAGALQPLARVLDTRALGELNA
                     EVARGRDPATLAEAFLEQALPEP"
     gene            21988..22851
                     /locus_tag="VNU01_08625"
     CDS             21988..22851
                     /locus_tag="VNU01_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWH32723.1"
                     /translation="MHRRTFLKSMGVLAGTVGTGGLLAACGDDEPTGTEPATEAAQLN
                     VIHASLETLAGEGRRFAFGLTTLSNVPVKAAEATVRVRTMDGAPVGEPASAEFHDEGG
                     SPLGLYVARIDVPQAGTYEVEVSAAEGVGTVAISVIAPEDSTVPVPGQAAVATATPTH
                     AAPLGVAAVCTNEPGCGMHEVSLDQALADGRPVALLFATPAYCTSALCAPAVNTLAEV
                     GDDRDWGDVAFVHVEIFSDEGQTVLQAVSDWGLRSEPWFFTIDRDGKVADRIDGPLLA
                     SELTQLVEAVA"
     gene            23041..23232
                     /locus_tag="VNU01_08630"
     CDS             23041..23232
                     /locus_tag="VNU01_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32724.1"
                     /translation="MIFGSHRKPEAVVLPYEAYEELRELAEELIVAQRLRERDARDSG
                     ARRSLRDVAGHFGIDLDTL"
     gene            23189..23626
                     /locus_tag="VNU01_08635"
     CDS             23189..23626
                     /locus_tag="VNU01_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32725.1"
                     /translation="MTSPATSASISTPCELLARPVTEDAVADVEDLRRTDERLLVEAL
                     RIAKRVDERTLAGKQLRYFSKTGDLTDCFRVYFGAAHGEDTHRMVFRELDDGAEVVEV
                     VVLAERDADIPYLLAALRLGRLDDPLRQADARRRIAKARRKRD"
     gene            complement(23646..24296)
                     /gene="thiE"
                     /locus_tag="VNU01_08640"
     CDS             complement(23646..24296)
                     /gene="thiE"
                     /locus_tag="VNU01_08640"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWH32726.1"
                     /translation="MTRDGQWRRRRLADARLYLCCDRTAGGGDLAGFLDAVLPAGVDI
                     VQLRDKHALEDDLFEAGPIFRAAALRHGALFVLNDEPLFAADLDADGVHLGQDDAPVR
                     EGRRAVGADRLVGLSTHAVEEIDAALDSDCDYFAVGPVWATPTKEGRPGIGLDPVRHA
                     AAVADRPWFVTGDMRVETIPEVRAAGGGRFVVVRAITGADDPADAVRAIRAAVRST"
     gene            complement(24293..24607)
                     /locus_tag="VNU01_08645"
     CDS             complement(24293..24607)
                     /locus_tag="VNU01_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32727.1"
                     /translation="MSEQQRLIDFEALTEQLRKAVAAIGMAAFVGAVAQTLRSGVDAG
                     ALLRWLTAFIALSVLAGAVLVAWHAYRGAVRSHGRGERLSGEDVGVVPPRAPRPRKPD
                     DS"
     gene            complement(24604..25413)
                     /locus_tag="VNU01_08650"
     CDS             complement(24604..25413)
                     /locus_tag="VNU01_08650"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWH32728.1"
                     /translation="MSATEQATARGDAFVIAGVTLRSRLIMGSGGAASLDALEQALRA
                     SGADLATVAMRRVDPAARGSVLDVLERAGCRVLPNTAGCHTARDAVLTARLAREALGT
                     DWVKLEVIGDDRTLLPDPVELLDAAEQLVDDGFTVLAYTNDDPVLAKRLERVGCAAVM
                     PLGSPIGSGAGIRNPYNLRIIVEAAEVPIVLDAGIGTASDAALAMELGCDGVLLASAV
                     TRARDPARMALAMRHAVEAGRLAGGAGRIPKRLYAEASSPFEGMAEYPGAP"
     gene            complement(25410..25616)
                     /gene="thiS"
                     /locus_tag="VNU01_08655"
     CDS             complement(25410..25616)
                     /gene="thiS"
                     /locus_tag="VNU01_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWH32729.1"
                     /translation="MTRVLVNGEARELDQPVPAAALRALGLDPGRAGVALALNGTVLP
                     RGQWHTAELHDGDEVEILTAVQGG"
     gene            complement(25613..26749)
                     /gene="thiO"
                     /locus_tag="VNU01_08660"
     CDS             complement(25613..26749)
                     /gene="thiO"
                     /locus_tag="VNU01_08660"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HWH32730.1"
                     /translation="MPTAAHAADVAVAGGGPIGLALAWRAAQRGLSVVVADPEPGGGA
                     AWAAAGMLAPVTEVHPGEEALLALNLASAARYPAFVAELEDVSGMDTGHRTEGTLVVA
                     RDRDDLEALEELRRLQASLGLASDRLTSRECRALEPGLAPSVRGGLLVHGDHQVDGRL
                     LVRALLAACERAGVRLVRSAVTALRRDGAAVTGVELADGAAVEAPQVVLAAGAWSAGI
                     AGIPDGLLPVRPVKGQIVYLRGPAGALAGRNIRGVEVYVVPRRDGRVAVGATMEELGF
                     DATPTAGAVHELLRAARELLPWVDELAFEDVVVSFRPATPDNGPLLGPTGVEGLLAAT
                     GHHRNGILLTPATADALAGCLSGGGLEGPAAAFDARRFGRVVAA"
     regulatory      complement(26740..26852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(26870..27220)
                     /locus_tag="VNU01_08665"
     CDS             complement(26870..27220)
                     /locus_tag="VNU01_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32731.1"
                     /translation="MAGTNGSDGFAAELAGLGFHLLQRDRQGLLQYARKPNRYLTEWV
                     HDDGRELLFTWEFELGEFCEAMGWQIGAAETSFQILYPQFDARLARDAAAVTGEIDRL
                     EQRLASLNLADPAL"
     gene            27317..>28265
                     /locus_tag="VNU01_08670"
     CDS             27317..>28265
                     /locus_tag="VNU01_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWH32732.1"
                     /translation="MEPGSPRTESLRDVLAGRYRLDARVGAGGMATIYRARDLTLDRD
                     VAVKVLHPHLMDDDGLRERFRTEARHAARLLHPNIVNVFDTGDREDPDGLPAGSPANL
                     PWIVMEYVDGPSLRDVLRERERLSPREALSITEPVARALARAHGGGVVHRDIKPENVL
                     IAADGTPKVADFGIARALAETSHTQTGTLIGSVHYMAPELVSGKPATAATDQYGLGVL
                     LYELLTGTQPLPAESPMAIALRHAQEAIPPPSAVNPEVPAALDAVVARATAHAAEDRF
                     PDMESFAAALRAAVPDGPEPVVVHRPVRHGGAQRGGEGLH"
BASE COUNT         3569 a        10699 c        10483 g         3514 t
ORIGIN      
        1 ggcgcagccc gggacggggc ggatcgcggg ggcggctgcg gtgctcatgc gtgctccgat
       61 tcgcggacga gcgggaacgc gtcgttggct cggacccgct cggcggcgcg gagcttggcc
      121 gagcgggccc gcgggttggc ggcgacctcg tcgccgtcgg gctgctcggc gccgcgcgtc
      181 accgggcgga ggagggggac gcggccacag ccgcacaccg ggagatccgg cgggcagatg
      241 cagccggtgg ccgcgtcgga gaagaaccgc ttggtgatgc ggtcctccag ggagtggtag
      301 ctcaggacgg cgatgcggcc gccctgagcc agcaggtccg aggcctgggg aagggaggcc
      361 tcgaaccgct cgagctctct gttcacggcg atgcgcagcg cctggaaggt gcgcgtcgcc
      421 gggtgcacgg cggagttgcg ccgcaccgcc gccggcaccg cgccgtagac gacgtcggcc
      481 agctgggtgg tggtgcgcag cggccgcgcg gcgacgatcg cccgcgcgat gcgcagggcc
      541 tggcgctcct cgccgtagcg gcggatcagg tcgaccagct cgtcgacgtc gagctcgttc
      601 accaggttcg cggcgctcac gtccgacgtc gggtccatgc gcatgtccag cggcgcctcg
      661 gcgcggaagc tgaagccgcg ctgcgcctgg tcgagctgca tggaggagac gccgaggtcg
      721 tagagcacac cgacgggggg gacgtctgca tgctctacga ctggcgtgac gtggtggttg
      781 atctggtcat accccgaatg aaccagggtc acacgcccgg cgtacgcggc caggcgccgt
      841 tccgccaggg ccagcgcatc cgggtcccgg tcgaagccga ccaggtgcag gtccgcccgg
      901 gcggccagca gcgccgcggc gtgaccggcg gcgccgatgg tggcgtccac gatcagcgcc
      961 gggccggggc gcaggccgtc caggagcagc tcgacgacgc gcgcggccat gaccggcagg
     1021 tgcggggcac cgccggcggg cacgtcgggc gtcgggtcca tgcggcgact ccaggtcgac
     1081 cggcaggccg ggcgctgcgc taggcgaaca gccacagggc gcccagcacg ctgcccagca
     1141 ccacgccggt ggaggtcagc acgagcagca tcagcagcgc cgaggccacg agctcgcgcc
     1201 cgaggtcgcc gttgaggtcc gcggggtggt ccacggtcgc ggtcagcaca tccatcagaa
     1261 gatccctcca aggtcgaacg gctgctcggt attggcgaag tccgccatgg cggagtcgcg
     1321 gtaggtctcc cagcgcccgg cgtcccagag ctcgacgcgc atgtcggcgc ccaccacggt
     1381 cacgtccttg gcgagggcgg cgtactcgcg cagccgggcc gggatggtga cgcggccctg
     1441 cttgtcgagc tcctccgagt gggcggagga ggtcatgacg cgggcgaaca tgcgctcgcg
     1501 gcggtccgtg gtccggagat tgcggaggct ggcgagcacg cgctgccagt catccacggg
     1561 gtggatggtc aggcagttgt cgagccccac cgtgatcacc aggccgtcct tcagtcggtc
     1621 ccggaaggcc gacggcagga tcacgcgccc cttggggtcc agcgtgtgct ggtactcccc
     1681 gaggaacatg cggcctcctc ccacgtccgt ccggtgctgt cacagggagg cggcatactc
     1741 ccccgccctc catagcgcac cactctccac cacttttccc cacatgtcaa caccgtggga
     1801 ccactctgcg cgactttttc gtcaccgcgc gcggcaaagc cgcaggtcaa ggcggtggtg
     1861 ccaagtgggg cgccccggcg cgtcccgggg gcccgggcgg cttccgcggg ctgcgggggc
     1921 aggccgggga ggccgctggg acggcgcgcg ggcgccctcg acggcgccgt aaaggggttt
     1981 tggcgccgta gagggcgtcc gcgatcagcc ggccggcggc ctcaccgggc cgaggtggtg
     2041 cgaagtgggg ggcggttccc cgttcaaacg ggtgcgggca cagcggcatg tcgcggtgcc
     2101 cgcacccgtt cgaatgggtg cgcgggtgag ggggctacag ggcggccagg gagtcgccca
     2161 ggtagggctg ccagtcgtcg tggaagccgc cgacggcgac caccagccag aacgcggcgc
     2221 gcggcgcgaa cggcgcgcgc cgcagcacca tgccgcactc ggccggcatc aggtccttct
     2281 tcttggagtt gcagcggcgg caggccgcca cgacgttctc ccactcgtgc gggccgccac
     2341 ggctgcgcgg caccacgtgg tcgacgttct cggcggcccg cccgcagtac tggcacgtga
     2401 agttgtcgcg aatgaagacg ccgcgtcggc tgagggccgc gcggcgacgg tagggcacgt
     2461 ggacgaagcg ccggaggcgt accacggagg gcgcggcgta ctccagccgc tcggagcggt
     2521 agcggtggcc gttggtctgg acgacgtcgg ccttgtcgtt cagcaccagc accaaggcgc
     2581 ggcgcgccgg cacgacgcag agcggctcca tggtggcgtt gagcaccagc gccttgtggt
     2641 gggggctcgg gcggcgctgc cgctccgcgc ggtgcgcgtg tcccgaggcg aggccgccca
     2701 ggccgcccgc cgcgacagcg ggcggttcac gatgcggatg cccggccgac cagtcggcca
     2761 tgacggccgc cgagtcgtcg cgcatccgca ggactggcgg gcctgcgtcc acacgtgcct
     2821 cctgccgccc ggaaccccct gccggcgcgg ccgagccact gtacccgctt tgacgcacgc
     2881 gctccgtccc ggcccgtggc cagctgcggg acgagttgtg tccgaagtcg gacttaagtg
     2941 gtcccgcagg ggggaacctg tcgggcccgc atggcgtcgt tagactggcc gcaccattcg
     3001 caccgccgag gcaagggaca tcgccgcgtg gcagcacgcc gaaccgtggg ccgccgggcc
     3061 gctgtggacg ccacgcagct cgccgagctc ttcgcccaga tcgagggcaa cgtcaccacc
     3121 gtggtcgaag gcaagccgga ggtcgtccgg ctggccctgg tcgccctgat cgcggagggc
     3181 cacgttctgg tggaggacgt gcccggcgtc ggcaagacgc tgctggccaa ggcgatcgcg
     3241 cgctccatcg cctgcagcgt gcggcgcgtg cagttcaccc ccgacctgct gcccagcgac
     3301 atcaccggcg tgacgatcta caactccgag cagggggact gggagttcaa gccgggtgcc
     3361 ctgttcgcca acatcgtgct gggcgacgag atcaaccgcg ccggaccgaa gacgcagtcg
     3421 gccctgctgg aggcgatgga ggagcacacc gtcaccgtcg acggcaccac ccacgagctg
     3481 ggcgtgccgt tcatggtgat cgccacgcag aaccccatcg agctggaggg cacctatccc
     3541 ctgccggagg cccagcgcga ccgcttcctg atgcgcctgg agatcggcta cccgtcgctg
     3601 gacgcggagc tggagatcct ggacgtgcac ggcaagagcg accgcctgga gggcatgacc
     3661 ccggtggcgg acacccgcca ggtcgcggcg atgatcgccg cctgccgcga ggtccacgcc
     3721 gccgacccgc tcaagcgcta catcgtggaa ctggtgcgct cctcccgccg gcaccccgcc
     3781 gtggagctgg gcgcctcgcc gcgcgcgtcc ctggcgctgc tgcgcgccgc ccgcgccctg
     3841 gcggccgcga gcgggcgcga ctacgtggtg cccgacgacg tgaagtcgct ggcgcgtccc
     3901 gtgctggccc accggctgat cctggccccg gacgcgcggc tgtcgcgcgg cggcgccgag
     3961 gaggtcgtcg acgagctgct cgactccatc cccatcccgg tgcagcgttg agggcgcgcg
     4021 gctgatggtc acggagcgcg gggcgggcct gctcatcggc gccgccttca cgtggctgct
     4081 cggccgcttc ctcggcgtgc cggagctgta cgtggtcgcc ggggcgggct gcgcgctggc
     4141 cggcctgtcg gtcgcctccg tgtggctggg ctcggcgcgg ctggacgccg gccgcaccgt
     4201 gtcgccggcg cgcatcgcct ggggcgggcg caccgtcgtg cgcgtcgcgc tgcgcaacac
     4261 cgggcggctg ccgtccgggc tgctgctggt gagcgacgag atcccctacg cgctggccga
     4321 cgcgccgcgc ttctccgtgc ccggcctggc gcggcgcgcg caggtggacc tgggctacga
     4381 gctgcacggc gccggccgcg gccgctacga cgtggggccg gcgcgggtgc ggctgcgcga
     4441 cccgttcggc ctggccgagc gcgtgcgccg cgtgggggcg accgccgagc tggtggtgta
     4501 cccgcgcgtg gagcgcctgg gcggcggcgt gcccgccgcc ggccggcagg gcgacggcgg
     4561 cgcccaccgc cgcacgctgc tgcacgccgg cgacgacttc tccacgatcc gcgagtgggc
     4621 ccccggcgac gacgtgcgcc tggtgcactg gccgtccagc gcccaccgcg accgcctgat
     4681 ggtgcgccag ttcgagcagc cctacgagcc gctggccacg ctggtgtgcg acacccgcgc
     4741 cctggtgcac cgcggcgcgg gcccggcgtc gaccctggag acggccgtga cggcggtggc
     4801 cagcgtggcc ggccacctcg ccgcgcacgg ccaccgcctg cgcctggcgc tgccggagga
     4861 ccgcgagacg ccgcccatcc agccgctgga gcggctgatg gaccgcctgg cgatggtgga
     4921 ggcgtcgcgc gagacgtcgc tggcgcccgc gctcggcggc ctggcgggac gcgcgtccgg
     4981 cgggctgctg gtcgccgcgc tggcgccacc cgccgacggc ggcgagcgcc tgggcgcgca
     5041 ccccgacatc cgcgcgctgc tggcggccgg gcggccctac agcgtgcgca tcgcgctggt
     5101 cgtggacacc ggcgccctgg ccggcccgcg gggaggccag gagcggcccg ccgcgctcct
     5161 ggccgccgcg ctgcacggag ccggctggcg caccgcgctg ctgcgccccg gcgcgcccat
     5221 gtccgacgcc tggaaccgcg tcgccggtcc cacggcgatg gtgctggggg gcgcgcgatg
     5281 accgccgtcg cgccacagcg gccgccggtc cacgccggcg ccacgccggc gcccgccggt
     5341 cctgtcacgg acccggccgc gcgcggaccc gccacgcccc ggccgccgct gcccggcgtg
     5401 gccgccgccg tggcgtgcgt ggccctgctg ctggcgaccg tcctgccgct ggaccgcgtg
     5461 tacgcggacc gctcctgggt cgcgccgacc gtgtccgcgg cgctgctggc gttcggggtg
     5521 ggctggctgg cgcggcgcct gcgcctgggg cccgtgctgt cggtgtcctg caacgtcgcg
     5581 gcctgggcgc tgttcagcgg cctggcgttc ctgcccgaca ccctgctcgc cggcctggtg
     5641 cccaccccgt ccaccgtggc ggcgggcgtg gccgcgtggg cgcacggcat cgagctgctg
     5701 cagctgcgcc cggcccccgc ggtgccggag acgggactgg tgctcatcac cgccacgggc
     5761 atctgggcgg tcgcccacgc ggtgcacgag ctggtgttcc gcgcccacgc gccgatgcgc
     5821 gccgtgctga tggcgatggt gatgtggatc gtgccgctgg gcctgtcgcc cgcgcgcggc
     5881 gaggcctggt tgtgggcggt gccgttcctg ggcgcggcgg cgctgctgct gctggcggcc
     5941 ggcgccgggg aggcggcgcg ctggggtcgc tggtcgccgc cggcgcaggg ccgcaggcgg
     6001 ctggggctgc ccgtcggcgc gcgcaacccg ctgggctggc tgctggccgc ggcggcgatc
     6061 gccgccggcg tcatgggcgc gccgctgctg cccggctgga ccgacccggc gctgtacgag
     6121 ctgcgcgacc tcggcggcag caccacgacc accaacccca tcgtcagcat ccgcgccagc
     6181 ctgctgtccc ccgacccgcg gccggtcctg caggtgcgca cgccgcggcc ggtgtacctg
     6241 cgcaccacgt cgttggaccg ctacagcgac cacgaggagt ggaccaacga cggcatccgc
     6301 ggcacgccgt tccgcgccgg ctccgtggtc ccgttcgagg tgccgatccg cacggcgctg
     6361 gacgtgcccg tggaggtctc ggtcgccgcg ctggacgcgg ccgtgctggt gccgatgccg
     6421 taccagccgc gcgtggtcga cggcaccatc ggcgacgtgc tgcagtacga ccagcgcctg
     6481 tcgacgttca cgctcaacaa gggcgtccgc ctcagccagg gcgacgtcta cgcggtgagc
     6541 gccgcggtgc ccgccccgct ggccgagcag ctcgaccggg tgccggccgg ctggtacgcc
     6601 ggcgagctca cgcggctccc gccgtccgtg ccggcggagg tcgcccagct tgcgcgcgac
     6661 atcaccggcc gggccggcgc ggtcacgccg ttccagcagg ccatggcgat ccaggaggag
     6721 ctgcgcgcct ggacctactc cctggacccg cccagcggcc acagcggcga ggcgatgcgc
     6781 acgttcctgc gcacgcgcac cggctactgc gagcagttcg ccgggaccat ggccgtgatg
     6841 ctgcgcacgc tgggcatccc ggcacgggtg ggcgtcggct tcacgccggg cgagctggcc
     6901 gacgccgccg ccggcgagta cctgatccgc tcgtccaacg cgcacgcctg ggtggaggtg
     6961 ctgttcccgg acgagggctg gatcgcgttc gagccgacgc cgcgcaccga cggcaacgtg
     7021 ctggtaccca gccccgccaa cctcgccccg tccgcgacgc aggcgcagca ggccggcgac
     7081 atccctgacg ccccggaggc cgacgacctg ccgcggcagg gtcccgagcg cctgccgacg
     7141 tccgccccca gcccggccgc caccagcccg tccgcgcccg gcgcgggcgg cggcgacggc
     7201 ggcggcacga ccctgctggc cgccgccctg ctgctgctgg ccttcgccgg cgtgggcggc
     7261 accgtgctgc ggcgcacgcg cgtggacgtg gacgccctgg gcccggcggc gcaggtgctg
     7321 cacgccgtgg ccaaggtgga gcgcgtctcg gcggcgatgg ggcgcccgcg caaggacacc
     7381 gagaccgacc gggagttcct gggccgcatc acccggtccg ccagcggccc cgcgctggcc
     7441 gcccaggccg agcgcgcccg ctacgccacc gagctgccgc tggagtccat caccctcgcc
     7501 aggaccgtgg cggcggacct ggtgcagcgc ctgctgtacc cgctggaccg ccgcagccgc
     7561 gccacggtgc tggcgcaggt gcgcctggcg gagctgcgcg agcgggcggc gcgcgccctg
     7621 ccgggccggt tccggcggcg aaagtgaacg gacgggtgtg ccgagacctt tcttgacgca
     7681 cactggaagg gctacggata ctcggcccgt cgatgaagcg gagtagctga tgcccctgtc
     7741 cgaccgcgag cagcagatcc tcgccgacat cgaggcgcgc ctgcgcgccg aggatccgaa
     7801 gttcgcgcgc gcggtgcaga ccaccaccgt ctcggcgcac ctgcgccggc gcatcaagct
     7861 gtccgccgcc gcgttcgtgg tcggcttcct gtccctgctg ctgctggtcc tgcacatcgg
     7921 cttcggcatg gccgggttcg cgctgatgct cgcgggggcc gtgagcggca tcaacgcgct
     7981 gaagcgcctg gggcaggccg agggtcccct gggcgaccag atccgcggcg ggttcgagcg
     8041 ctaccgctcc gaccgcgacc gcgaggagta gcgggcgcgg ccggcgggcg cgctcggcgc
     8101 ctgcttaccg gcggcgtcgt tgcgggtcgc gccactggaa ccactgcagc gggccgccct
     8161 cgaaggccgg ctcggcgtgc tgcacggcga aacccaggcg cgtgtaccag tccttggcgg
     8221 tggggcgggt ggtctccagg aagcacggct cgccgccgtc gtcggcgcgc cgcaggccgg
     8281 gtgcgatgag gcgcgcgccc accccgcggc cctggtcggc cgggtcgacg gcgatcgccg
     8341 cgaggtacca gtgctcggga tggtccggat ggggacgctc cacgcgcgcc agcgcctgcg
     8401 cgaccaggcg cgcccgccgc gggtagcggc gcagggccgt cgcggcggcc ggcaggaacg
     8461 ccacggtgcg ccccgcaggc atcgggtagg cgccgggccg cagccacgcg cagtagccgg
     8521 ccaggcggtc gccgtccagg gccacctcca ccacgccgtg ggcgaggcag tcggccacgg
     8581 tggcggcgta gaagcggcgc gcgatcccgg cgcgccgagg gtcgccggcc tcgcccggga
     8641 acagctcgcg catgaacggg tagtcgtcga aggcgcgggc ggacagcccc gccacctgcg
     8701 ccatgaccgg tttcgccgag gcgcggtcca gccgcgtgac ggcgagctcg cccatcggtg
     8761 ccgctagcgg ccggcgaagt ccggcgcgcg acgctgcatc gccgccgcga ccgcctcgcc
     8821 gaagtcggcc gaggacaggc agccggcctg ggtggcgcgc tcggccgcca gggcgtcctc
     8881 acgcgtgcgg tccaggctct cgcgcatgag gcgcgcggcg ccggcctgcg cgagcgtcgg
     8941 gccggcggcg agtgcggccg cgtaggagcg cgccgcggcg tcgaagtcct cgtcgtcgag
     9001 cacggcgtcc acgaggcccc acgccagggc cgtcgccgcg tcgacgcggc gggcgctgac
     9061 cgccatgtcg atggcgcgcg acaggccgac caggcggggc aggcggtagg tgccgccgag
     9121 gtccgggatg atcgcccact tcacctcgag cacgccgatg gaggcgtcgg gcgcggccac
     9181 ccgcaggtgg caggcggccg cgagctggca gcccccgccc agggcgacgc cgcgcacggc
     9241 ggcgacggtg ggcaccggca ggtcctccca ggcggtgaac gcctgctgga gccaggcgat
     9301 catgccgtcc tgcgtgccgc cgccggccgc ctgcagggac gggtcgccgg cggccatctc
     9361 cccgaacagc gacacgtcca ggccggaaga aaaggcgcgg ccggcgccgc gcagcagcac
     9421 ggccttcacg gcgccgtcct ccgcggcggc gcgcgcctgg gcggcggcgt cgtggagccc
     9481 ctcgaacacg ccgcggtcca gcgcgttcag cttgtcggga cggttcagcg tgaccacgcc
     9541 gaccttgtcg tcgccgacct cgaacatcac ccggtcactc atgcgatgga gcctactccg
     9601 cattcccaat ggtcagtgct gccagtcctc gcggcgggcg gactcgtcgc cgctggtggg
     9661 cgcggcgtcg cgcagcaggg cgccgcgcgt cacggcctcg ccgccgaagc ggtcccgcgc
     9721 cacgtcggcg gcgcgctcca ggcggctcca gcggtcgtcg gcggtcaggg agagctggcg
     9781 gctggcgtcc tccggcacga ggttggtgac gccgacgccg atcaggcgca cccgcacccg
     9841 ctccaggcgc agcccctcca gcagggccag cgcctcgcgg tgcagttccg tggcctccgc
     9901 cgtcggcatc agcagggtgc gggaccgggt caccgtctcg aagctggcgt agcgcagctt
     9961 cagcgtgacc gtgcgcccgg cgacgttgcc cttgcgcagc cgccgcgcga ccttctccga
    10021 cagccgcagc agctcccggc gcagcaccgc gaggtcatcg acgtcgtggg cgaacgtctc
    10081 ctcggcgctc atgcccttgg ccggctcgtg cgggaccacc gggcggtcgt cgcggccgtc
    10141 ggccagcgtg cggatctggt gggccgccgc cgggccgagc agccgcgcca gcgtcgcctc
    10201 cggcgtcgcg cgcacgtccg cgagcgtgcg gatggcgaag cggtccagct tcgcggcggt
    10261 ctgctcgccc acgccccaca ggtcgcgcac gggcagcggc gccaggaagg cgccgacctc
    10321 ctcggcgcgc aggtgcagga tcccgtccgg tttcgccttg ccgctgcaca gcttggccag
    10381 gtacttgttc ggcgcgatgc cgacgctgga cggcaggccc agctcgtcgc ggatgcggcc
    10441 gcggaccagc tcggcgatgc gcggcggcgg ccccagcagg cgcacgctgc cgccgacgtc
    10501 caggaacgcc tcgtccagcg acagcggctc gaccagcggc gtgaccgcca ggaagatctc
    10561 gcggatccgg ccggacacgc gctggtaggc gtcgaagtcc ggcggcagca ccacggcgtc
    10621 cgggcacagg cgcagcgcgc gcgccatcgg catggcggag cgcaccccgg ccgcgcgggc
    10681 ctcgtaggag gcgctggcca cgacgccgcg cccgccggtg ccgccgacga gcacggggcg
    10741 gcccagcagc gccgggtcct tgcgcgcctc gacggacgcg tagaacgcgt ccatgtccac
    10801 gtgcaggatc ggctcggcga cggccacccg ccgagcgtac gtgcgccggg cgagcgtcgc
    10861 gctcatcccg ccgagccgcc ggaccgatgc cacagcgggg tttgtctctc cccaatgcgc
    10921 gggggcatgc ccgcgatgcg cccctggcgc agcaccgcgc gcaggcggtc gccgcgccac
    10981 gcgtccagca ggtcgcgcaa gtcccaggcg ctgtgcgcgt tgacggacac ggcgcgcccg
    11041 ccgccgtggc gcacgcggcc ctccaccacc agcagccagg agtggaagac ggtgtgcgcg
    11101 cagcgctcca gcacgcgttc gaagaacgcg aggtcggcca cgccggtggc gtcgtccagc
    11161 gacaggaaca ccacgcgctg cccggagcgg atcggcgggg tctgcgtggc gaccttgacg
    11221 cccgcgacgc gcaccagcgc gccgtcgggg cggtccagca ggccggcggc gcgcgtcacg
    11281 ccgagcgcct ccagcagcgg ctcgtagaag ctcaccacat gggcgctggc gtccaggccc
    11341 agcacctcga gttcggcgcg cacgcgctcg ctgagggtgt agtcggccag cccggccgcc
    11401 gcctcggcga ggagctcgcc ctgcaccggc ttggccgccg cggccttcgt gcgcgcgcca
    11461 ctccagcgct cggcgacctc cagcacgatg tcgcggcggg agcgcccagg cgccaggcac
    11521 tccagcgcgc cgacgtgcgc gagcgcctcg gcgaccgggg ccgacagcgc cgaacggtcc
    11581 cgcaggtccg ccaccgacgt gaacggccgg tgcgccagga ccgactcgat catgcgctcg
    11641 tcgatccccg ccacatcgca caacgccagc ctgatgccga accgcaccga cggctccgcc
    11701 ggatcactcc ccaggtccgg cacagcagac gctggagccg ccgggaggga cggggtgggg
    11761 ccggcccatc gaccggcgcc gacgtaaaga gggtttccgg aggcgccggg agaatgggac
    11821 ggccccaccg gcacaccggg ctcccttgcg ccccccgccg gacccgttgc gcaccccgcc
    11881 atcccggcgt gatctgccgg cacacgctcg acgcggtagt cgcgggcgga gcggttgacg
    11941 tccagcggca gcacggcgac gcccagccgg cgcgcgtcct ccaagagcag ccgccgcggg
    12001 tacatgccgg gatcgtgggt gagcagcccg gcgacgaact ccggcaggta gtggcgcttg
    12061 agccacgccg agcgccacgt cggcacggcg aacgccgccg cgtgcgcctt gcagaagccg
    12121 aacgaggcga actgctccag cgcgtgccac agcctgcgcg ccgtcgcctc gtcgatgccg
    12181 cgctcggcgc agccggccag gacccagtcg cggatctcct ccaggcgctc ggcgtcggac
    12241 aggcgccggc gcaccaggtc ggccaccgcc aggtcgcagc cggtcaccgc ggccacgcag
    12301 cgcatcacct gctcgtggaa cacgatcacg ccgtgggtct ccgccagcgc ccgctccagg
    12361 atcgggtgcg ggtagacggt cggctcgcgc ccgtggcggc gcgcgacgaa cggccgcacc
    12421 atgtcgccct tcaccggccc ggggcggaac agcgagatgt cgaccacgag gtcgtgcagc
    12481 tcggcgggct gcagccggcc gatcaggtcg cgctgcccgg gcgactcgat ctggaacatg
    12541 cccagcgtgc gcgtggagcg gatcagctcg taggtgtcct cgtcgtgcca cgggatcgcc
    12601 gacagggcca cgtcctggcc gcgcacccgt cgcacctcgt ccacgcagtg cgacattgcc
    12661 gacagcatgc gcaccgccag cacgtccagc ttgcacaggc cgagcgcctc gacgtcgtcc
    12721 ttgtcgaact gcgccatcgc gaagccgtgc gcggaatgct ccatgggcac caggtcgcgc
    12781 agggtggcgt cggccagcag cacgcccgag gggtgcagcg cgatgtggcg cggcaggccg
    12841 tccagcgcct ccaccgcgtc gaacaggaac gccaggtccg gccggtccag gttgcgcccg
    12901 cgcagctccg gcaggtggtc cagccccttg cgcacgtcgc gggcgcgcag gtgcgggaac
    12961 gccttggcga tcacgtcgat ctcggcctgc ggcaggccga tcgccttgcc ggcctcgcgg
    13021 atcgccatgc gcgcctggaa cgtctccacc atggcgacgc aggcgacgcg gtcgtcgccg
    13081 tagcggtcga ggatgtcgcg gtagatgtcc tcccggcgcg ccgactccac gtcgatgtcg
    13141 atgtcgggca gctcctcgcg gtaggggttc atgaagcgct cgaacgcgag gtcgtgcgcg
    13201 atcgggtcga cgtcggagat gcgcagcgcg tagatcagga tgctgccggc ggccgagccg
    13261 cggcaggcgg cgatgatgcc cttggagcgg atgcgcgcga cgatctcggc gacggtgagg
    13321 aagtacggcg ccaggcccat gcgcgcgcac atcgcgagct cgcgctcgaa cgtgtcccag
    13381 tggcgctcgg ccgggctggc gtagcgctcg cggatgccgg cctcggcgcg cgccttgagc
    13441 tccgccagcg cgtcctgccc cagcgcgacg aagtcgggca cgcgcaggcg gcccagctcc
    13501 agctcggcct cgcagcgttc ggcgacccac gcggcgttgg cgaccgcgtc gggacgctca
    13561 cggaagacct ccgccatctc ctcaggcgtc ttgagatagc cctggtcgtt ggcgcgcccg
    13621 cgcgccacgg tgcgcagcgg cgcctggagg cgcacggcgt cgagcacgtc ggcgatgcgc
    13681 gcgtgcgcgg cgtcgaggta gcgcggctcc tggttcgcca cggcgcgcag gcccagccgg
    13741 tcggccagcg cgaagcgcgc gcgggcgcgg gcgtcgtcgc cgggcgccag gtggtgcgtc
    13801 acgccgacga gcacggagct cggcccgtac acgtccatcc agcggcgcgc cccgacgccg
    13861 ccgcggcgtc ggggcgcccg gcgtcgagca ggcggccgac cggggagtcg tcgccgagca
    13921 gcacgaactg cgcgtccggg cggcgctcca tctgcgccca cgtcagccgc ggcgtcccac
    13981 ggacggactc caggtgcgcg tcggacacgg tgcgcgccag gttggcgtag cccggcgtgg
    14041 tgcgcgcgat cagggtgacc cgcgagggcc cgtcgtccag ccacgccggg ccgctgccgg
    14101 gaccggcgaa gcggccgcgc tggacgcgcg ccgcccagcc gggccgcgca tcgtcgggcc
    14161 gcagcgccag gtccgccccg tagatcggcc gcaccccggc ggcctggcag gcctgcgcca
    14221 cgcgcaccgc cccgtacagg ccatcgcggt cggccacgcc cacggcgcgc atgccgcgtt
    14281 cggccaccgc ctgggccagc tcgccgggac gcagcgcccc gtggcgcatc gagtagcagg
    14341 tccgtgcggc gaggtgagcg aacatgtgtt cgattctgct cgcgaacgca cccgcccaac
    14401 agggtggaga gcggtgtacc gatagcccgc cgagcggtgc ggcagcaagt ttgacctcgc
    14461 gccgtgcgcg gaacccccgt accctctgcg cttccccgcc gcacccacga ggagcccgca
    14521 tggagccggt ctacacgccc gtcatcgggg ccgcgaagac gctcatcgcc gccatgaact
    14581 ggaagatcca gatccacggc gccgagcaca tccccgcgtc gggcccggcg ctgctggcgt
    14641 ccaaccacat cggctacctg gacttcgtgt tcatcggcta cggcgcgctg gaccgcggcc
    14701 gcatggtccg cttcgccgcc aagaaggagg tcttcgacca caaggtctcc ggccccctca
    14761 tgcgcggcat gcggcacatc caggtggacc gcttcggcgg cgcggacgcc gccgtggtcg
    14821 cctcggtcgc cgccatgcgc cgcggcgagg tgatcggcat gttcccggag ggcaccatca
    14881 gccgctcgtt catcccggcc gccggcaaga ccggcgcggc gcgcatggcg atggacgcgc
    14941 aggcgccgct gatccccacg gcggtgtggg gcacccagcg catcctcacc aagggacgcc
    15001 cgaagaactt ccagcgcggc gtcgtgatcg acgtgcgcta cggcgagccg gtcgcctgcc
    15061 agcccggcga cgaccccacc gaggtcacca agcgcctgat ggacgccatc accggcctgg
    15121 tcgagaacgc cgcgcgcgag tacccgcagc ggccggccgg cgacggcgac cgctggtggc
    15181 tgccggcgtc gatgggcggc accgcgccca ccgtcgagga ggccgaggcg atggccgcgg
    15241 cggaacgggc cgagcggcgc gcgaagcggc agcgcgaggg ctgaaacccg gccgccccgc
    15301 gcggcgtctc accggcacga cacgagcggg aggtgggacg atgcggatcg cgctgctcac
    15361 ggtgacgttg gcgctggccg cggcgtgcgc cggcccggac ggcgcggagg tgtccttcag
    15421 cacgccgccg gacgacgccc gcacgcaggc cgtgccgccg gaaccggccg ccacgaggga
    15481 ggcggccacg ccgggggccg ccacggcgag cgcggtcccg gcccagacgc cggggccggc
    15541 ggccccgcgg accgtcccgg cctcgcccac accgtccggc gggccgcgcg agctgggcgc
    15601 ggacgaggac gccaaggtcg gccagccgta cctgtaccgc ctgtacacgc actgcgggat
    15661 cgaggcgacg tggtggaacg gcgcctggtg ggacgccgac ccgccgctgt cggacggcag
    15721 cggcaacccg ccgcccgggt ggggcaaccc gtaccaggac ggcgtgatcg tgcaggtctc
    15781 cgagacgcgg ctggagttca ccggcgacga gggacagcgc gccgccttcc ggccgcggcc
    15841 ggccgacgtc ggcgccccac ccccctgcga ctgaccggac gtccacagat ttcagccggg
    15901 tatgggtgca ctttcagagc gatgactctg agagtgcacc cagcgacggt cagggcagct
    15961 cggtggtcgc gtccgggtcg atgccatagc gctccactgc ctcgcgcacc agcttggcct
    16021 ccaccgcgcc ctgtttggcc agctcgtgca gcgccgccag cgcgacgtgc tcggcgtcga
    16081 tgcggaacag ccgccgcagg gccgagcgca ggtcggaccg gccgaagccg tccgtgccca
    16141 gcacgtggta cggcgccggc acccacggcg ccaccagcag cggcaccgcc ttctggtagt
    16201 ccgtcacggc gatcaccggc gcgtcaccgg ccccgcgcag cgcctcggtg acccacggca
    16261 cgcgcggctc ctcctccggg tgcagcaggt tccactcgtc gcacgcgacg ccctcgcggt
    16321 gcagttgcac ccagccgggg gcggaccaca cctcggtgcg cacgccccag tcctccgcca
    16381 gcaggtcgcg ggcgcgcagc gccgccggca tcgccgagcc gctggtcagc acgcgcgcca
    16441 gcggcccgtc gccgtcgggc gcctccaggt agcggtacag gccgcgcagc accagccgct
    16501 cgtcgaggcc gtcgggcatc ggcggctgcg gcaccggctc gttgtagacg gtgaggtagt
    16561 acatgaggtc agcaccatgg cctaccgctt cgctgcttga ggccggaccg tcggggtcgg
    16621 cctggttgcc gagcatcctg ttgatgccgt cctccacgac gacggcgatc tcgtaggcga
    16681 agctcgggtc gtaggacacc accgcggggt tggtggccgc gtacagcagc gagtggccgt
    16741 cctcgtgctg cagcccctcg ccgttcagcg tggtgcgccc ggccgtcgcg ccgatcagga
    16801 accccttgca gcgctggtcc gcggccgacc agatgaggtc ggcggtgcgc tggaagccga
    16861 acatcgaata gaagacgtag accggcagca tgtgctcgct gaacgtcgcg tacgccgagc
    16921 cggcagcgtg gaacgacgcc atggagccgg cctcggtgat cccctcgtgc aggatctggc
    16981 cttgcgtgga ctcccggtag gccagcagca tctcgcggtc caccggctcg taggtctgcc
    17041 cgtgcggcga gtagatcttc tccgtgggga acatcgcgtc catgccgaac gtgcgcgcct
    17101 cgtccgggat gatcggcacg atgcgcttgc ccaggtccgg gtgcttgagc aggtccttga
    17161 gcagccgcac gaacgccatg gtggtggcga cgtcgtgctt gccggagccc tgcttcagtt
    17221 ccgcgtaggc gtcgcgctcc ggcaggctga ccgggtgcac gcgcacgcgc ctggccggca
    17281 gcggcccgcc cagctccagg cgccgctcca gcatgtactg gatctcgtcg gagtcctcgc
    17341 ccgggtggta gtacggcggc aggtcgctct ccagcgcctc gtccgggatg tccaggtaca
    17401 gccggtcgcg gaacttcttc agcgccttct gcgtgagctt cttcatctgg tgcgtggcgt
    17461 tgcgcgcctc gaagtccggc cccagcgtcc agcccttgat ggtctgcgcc aggatcacgg
    17521 tgggctggcc ggcgtggttc acggcggcct cgtaggcggc gtagaccttg ccgtagtcgt
    17581 ggccgccgcg gtccagcacc ggcaggtccg cctccgtcag gctggactgc tccaggatgc
    17641 gccgcagctc cggggagtcg aagaagtgct ccctgatgta ggcggcgtca ctgacgctgt
    17701 aggtctggaa ctgcccgtcg ggcgtcgcgt tcatcttgct gaccagcgcc cccgtggtgt
    17761 ccagcgccag cagctcgtcc cacttgcggc cccacaccac cttgatgacg ttccacccgg
    17821 cgccgcggaa cagcgactcc atctcctgga tgatcttgcc gttgccgcgc accgggccgt
    17881 ccaggcgctg caggttgcag ttgaccaccc agatgaggtt gtccagctcc tcgcgggcgg
    17941 cgaccgtcag cgcgccctgg gactcgggct cgtccatctc gccgtcgccc acgaacgccc
    18001 acacgcgcga gtcgccggtg tcggcgatgc cgcggtggtg caggtagcgg ttgaagcggg
    18061 cctggtagat ggagttgatc gggcccagcc ccatggagac ggtggggaac tcccagaagt
    18121 gcggcatgag gcgcgggtgc gggtagctgg gcagcccggc gcggccgtcg gacgtgcggc
    18181 gcgtctcctg gcggaacagg tccagatgct cctcgctgag ccgcccctcc aggtaggcgc
    18241 gcgcgtagat gcccggtgag gcgtggccct ggaagaagat ctggtcgccg tggcgcgggt
    18301 cgtcggacgt gcccttgccg cggaagaagt ggttgaagcc gacctcgtac agcgacgccg
    18361 acgaggcgta cgtggcgatg tggccgccga cgccgatgtc gggccggttc gcgcggctga
    18421 ccatgaccgc ggcgttccag cggatgtagg cgcggatgcg ccgctcgatg tactcgtcgc
    18481 cggggaacgg cggctcgcgg tccggcggga tcgtgttgat gtagtcggtg gagcgcaggt
    18541 gcggcacgcc gacctgctgc tcgcgggcgc gttccagcac gcgcagcagc aggtagcggg
    18601 cgcgctggcg cccctcgcgc tggaccaccg cgtccaggga ctcgatccac tcgcgggtct
    18661 cctgggggtc cacgtcgggg agctgggtcg gcatcccgtc cgtgatcact ggcttgcggt
    18721 cggtcacagc gctggtcctt cctgcgtgca cgtgcgcctc gcgcgccatg tcgcggcggt
    18781 cgggcgccct tttgcgccag tcttccacca tcggcggggc cgcgccgggt accttgcccg
    18841 ctgttcccga tgaccaaaga cccaggcgag gcgatgcccg tgcacggcgg acggacgtgg
    18901 gtgctggcgg ctgtgctgtg cgtcgccctg gccgcctgcg gcggcggggc ggagcccggc
    18961 tcggagacgt cggtgttccg catccggccc ggcgactgct tcaacgaccc gccggcggag
    19021 cggcagctgg accaggtcga cgtggtgcgc tgcgaccagc cgcacgacaa cgaggtgttc
    19081 gcggcgatcg agcaccccgc cgcgccggag gagccgttcc ccggccgcga cgcgctggtg
    19141 ggctacgccg agcagggctg cccggagccg ttcgcggact acgtcggcgc gacgtacgac
    19201 cagtcgcgct tctcgctgtt cccgatcgtg ccgagcgcgg acacgtgggc gaagggcgac
    19261 cgccagatca tctgcgcgct gtacgaccac cgggccggca agctgaccgg ctccgtgcgc
    19321 ggctcaggcc agtagcgcgc ggacgcggcg cagatcatcg acgaagtcgg cgcgctgcgc
    19381 ggcgcggcgc tcgccggcgc gcaggatcga cgaggggtgc acggtggcga cgtagcgcac
    19441 gccgtcgcgc tcgtgcacct ggccgcgctc gcgcgtcacc cggaaggagc gtcccagcag
    19501 gccctgcgcg gcggtggcgc cgagcggcac gaccagttcc gggcggacca cggcgagctc
    19561 cgcctccagc cacggccggc acgcctcgac gtgctcggcg ttcggcttgg cgtggatgcg
    19621 cttgccgccc ttgcccgcct gcgctttcca gcggaagtgc ttgacgatgt tggtgaggta
    19681 gacgtcggcg cgggcgatgc cggcctcctc cagggcctcg tccagcaggc ggccggccgg
    19741 ccccacgaac gggtgcccct cgcggtcctc gcggtcgccg ggctgctcgc ccaccagcag
    19801 cagccgcgcc cgccgcggcc cctcgccgaa cacggtctgc gtaccggtgc gccacagctc
    19861 gcaggcctgg cagccggccg cggcctcgcg cagcttcggc agcgtggggc gctccgggac
    19921 cagcgccgcc gccgtggcgc tcacaccagc cccgcgtggt cgtcggcgtg caggccgccc
    19981 tggctgtcct ggcgcgtcgg cagccgcagg atctggggca gcagcgtctc ggcgatgtcg
    20041 tcgcgcaggt cggtcagcgc ctccagcggc cggtcgctgt cgtcgatgcg caggtcccac
    20101 agcgggtagc gctcggtggc caccacgagg accgacgccg agcgggcgcc gcgccggtcc
    20161 ccgccggcct tctcccccgc gatcagcgcg ccgatgagcc ggtcgacgag gtcgctgccg
    20221 ggcgacccgg cgtaggcctc caggcaggcg tccagcgtct cggggccctc cagcaggttg
    20281 ccctggacgg cgacgtcctg ctcgctgcgg tggccggccc agcgctcgca gcgctcgccg
    20341 gtgtaggcgg cgctggtgcc gtgcgcgtcg accatgccga cctggcgcag gcgccggtcg
    20401 tcgtccatgg cgaccacggc gcgcagcgtg cgctctgcgg ggtggccgtt gcccatgagg
    20461 tccagaccgt cgacgcccag gtaggggttc acccaggcct gcgtggccac cgcgccgacg
    20521 cccgcctccg cccaggtgac gagctggccg acgccgggcg tgccggtcac cgcaccgacg
    20581 ccgagctgcc cggtgcgcgc gcagcgcgcg acgatcgaga acgtcatggc gtgctgctac
    20641 ccggtgcggc tcagtcccag acgcggtcca gccgccaggc gccgccctcg cgcaccagct
    20701 cgtagacgcc ggcgcccacc tccacgcgcc acaggtcgcg ctggggcacc gccatgccgc
    20761 cgccctccca ccagccggcg tcctcccgcc agtggcccag cacgccccgc acgcggtagg
    20821 cggcgccacg ccagcggaac gccgacgggg cgccccgctc ggtggtctgc acctcgacgg
    20881 gctcgcggta gcgcttgctc atcgccgcct ggcctcgcac cctgtcgaac acatgttcgc
    20941 tacggtaccc ggtcggcggg cgcggcacca gcccggccgg ccggctacgg ctccggcagc
    21001 gcctgctcca ggaacgcctc ggccagcgtc gcggggtcgc gtccccgggc cacctccgcg
    21061 ttgagctccc ccagggcgcg cgtgtcgagc acgcgcgcca gcggctgcag cgccccggcc
    21121 aggcccggat ggcgcgccag cgcggcggcg cgggccaccg gcgccaccac gaaggccggg
    21181 aagatcgcca gttcgtcggc gacgggcacc agcccggcgg cgcgcgcctc cccggacgtc
    21241 gccgtggcca gcccggcgaa gcacgagccc gccgcgacgc ggcggatcgc cgtggcctcg
    21301 tccgccgcga ccacgcgcag gcggtccatg ccaatggcgt aggcctccgc cagcgcgtcc
    21361 aggccgccgt cgcggcgcac gaagtcgcgg tcggcgcaca gggcggcctc gccgccggac
    21421 agcacgccgg ccagccagct cataccgcgc ggggagccct ccggcggcag cgcgtcgccg
    21481 cgcacgtaca gcgccagggt cgcgttggcc gcggtcggct ccagccacac caggccgcgg
    21541 tcctcgtcgg cgcggcgcac gcgctggtag ctctcgtcgg tgtcggcggg cggggcctgc
    21601 tcccccatgc cgagcgccca cgccgcgccg gtgtagtccc accacagctc cacgttgccg
    21661 cgcaggccct cgcgccgcag gcccacggtg ccgcccaggt cggggcgggg ctccacgcgg
    21721 atgcccgccc ggtccagcgc caccacggtg agcgcggcga gcatgcgctg ctcggcggtg
    21781 gagcccacgc ccagggtcac caccgcgtcc tcaccgccgc cgcggcagcc ggcgacggcc
    21841 gcgcacagcg ccagtgacgc ggccagaacc cgacggcgac ccatgccggc cagtctgcca
    21901 gttgggcgcg cccggccggg ggcgactacc ctagtgcggg cgcgcccgcc gccactcccg
    21961 cctcctggag acaaggaccg tgttcgcatg caccgacgca cgtttctgaa gtccatgggt
    22021 gtgctggccg gcacggtcgg caccggcggc ctgctggccg cctgcggcga cgacgagccc
    22081 acgggcaccg agccggccac ggaggccgcc cagctcaacg tgatccacgc gtccctggag
    22141 acgctggccg gcgagggccg ccggttcgcg ttcggcctga ccacgctgag caacgtgccg
    22201 gtgaaggccg ccgaggccac cgtgcgcgtg cgcaccatgg acggcgcgcc ggtgggcgag
    22261 ccggcgagcg cggagttcca cgacgagggc ggctcgccgc tgggcctgta cgtggcgcgc
    22321 atcgacgtgc cgcaggccgg cacctacgag gtcgaggtca gcgccgccga aggcgtcggc
    22381 accgtggcga tcagcgtgat cgcgccggag gactccactg tgccggtgcc cggccaggcc
    22441 gcggtggcga cggccacgcc gacgcacgcc gcgccgctgg gcgtcgcagc ggtctgcacc
    22501 aacgagcccg gctgcggcat gcacgaggtc agcctggacc aggcactggc cgacgggcgc
    22561 cccgtcgcgc tgctgttcgc cacgcccgcg tactgcacca gcgccctgtg cgcgccggcc
    22621 gtgaacacgc tggccgaggt gggcgacgac cgcgactggg gcgacgtcgc gttcgtgcac
    22681 gtggagatct tctccgacga gggccagacg gtgctgcagg cggtgtccga ctggggcctg
    22741 cgcagcgagc cgtggttctt caccatcgac cgcgacggca aggtcgccga ccgcatcgac
    22801 ggcccgctgc tggccagcga gctcacgcaa ctcgtcgaag ccgtcgcctg acggcccgcc
    22861 ggtcgccagc ggacctcgct tgcggcgctc accacctcgc agatgttctg ggcagaaaga
    22921 tgtacgtctt tctgcccagg agatggtgcg ttggtgcatc cggtgacgac gacagaggcc
    22981 cgtgaaggcc tgacttcgac ggtgcgccgc ttccgcgagg aaggcgtccg agcggaacca
    23041 gtgatattcg gctcccatcg caagcccgag gcggtggtcc tgccgtacga ggcgtacgag
    23101 gaactccggg agctcgccga ggagctcatc gtggcgcaac ggctgcggga gcgcgacgca
    23161 cgcgacagcg gggcacgccg gtcgctgcgt gacgtcgccg gccacttcgg catcgatctc
    23221 gacaccctgt gagcttctcg cgcgtccggt caccgaggac gcggtcgccg atgttgagga
    23281 cttgcgtcgc actgatgagc gtctgctggt ggaggccctg cgaatcgcca aacgcgtcga
    23341 cgagcgcacc ctcgccggca agcagctccg ctacttctcc aagaccggcg acctgacgga
    23401 ttgctttcgc gtctacttcg gcgccgcgca cggtgaggac acgcaccgga tggtgtttcg
    23461 ggagctcgac gatggcgccg aagtcgtcga ggtcgtggtc ctcgccgagc gggatgcaga
    23521 catcccgtac ctgctcgcgg cccttcgcct tggccggctg gacgacccac tgaggcaagc
    23581 agatgcgcgg cgacggatcg cgaaggcgcg caggaagcgc gattgagtgc ggcgcgccgc
    23641 cgagctcaag tgctccggac ggcggcgcgg atcgcgcgga cggcgtccgc ggggtcgtcg
    23701 gccccggtga tcgcgcgcac caccacgaac cggccgccgc cggcggcccg cacctcgggg
    23761 atcgtctcga cccgcatgtc ccccgtcacg aaccaggggc ggtcggcgac ggcggcggcg
    23821 tggcggaccg gatccaggcc gatgccgggc cggccctcct tggtgggcgt cgcccacacc
    23881 ggcccgaccg cgaagtagtc gcagtcggag tccagcgcgg cgtcgatctc ctcgacggcg
    23941 tgcgtggaca ggccgaccag ccggtccgcg cccacggcgc gtcgcccctc gcgcaccggc
    24001 gcgtcgtcct ggcccaggtg cacgccgtcg gcgtccaggt ccgcggcgaa cagcggctcg
    24061 tcgttgagca cgaacagcgc gccgtggcgc agcgcggcgg cccggaagat cggccccgcc
    24121 tcgaacaggt cgtcctccag cgcgtgcttg tcgcgcaatt gcacgatgtc cacgccggcg
    24181 ggcagcaccg cgtccaggaa gccggcgagg tcgccgcccc cggccgtgcg gtcacagcac
    24241 aggtagagcc gggcgtcggc caggcggcgg cgccgccact gcccgtcccg cgtcacgaat
    24301 cgtccggctt gcgcgggcgc ggcgcgcgcg gcggcaccac gccgacgtcc tcgccggaca
    24361 gccgctcacc gcggccgtgg ctgcgcacgg cgccgcggta ggcgtgccag gccaccagca
    24421 cggcgcccgc gagcaccgac agcgcgatga acgcggtcag ccagcgcagc agcgcgccgg
    24481 cgtccacgcc gctgcgcagc gtctgcgcga ccgcccccac gaacgcggcc atgccgatcg
    24541 cggccaccgc cttgcgcagc tgctcggtca gggcttcgaa gtcgatcagc cgctgttgct
    24601 cgctcatggc gcgccaggat attcggccat gccctcgaac gggctcgatg cctcggcgta
    24661 cagccgcttg gggatgcgcc cggcgccccc ggccagccgg ccggcctcca cggcgtggcg
    24721 catcgccagc gccatccgcg ccgggtcccg cgcccgcgtc accgcggacg ccagcagcac
    24781 gccgtcgcag ccgagctcca tcgccagtgc cgcgtcgctc gccgtgccga tgccggcgtc
    24841 caggacgatc ggcacctcgg ccgcctccac gatgatgcgc aggttgtacg ggttgcggat
    24901 cccggcgccg ctgccgatcg gcgagcccag cggcatcacc gccgcgcagc ccacgcgctc
    24961 caggcgcttg gcgagcaccg ggtcgtcgtt ggtgtaggcc agcacggtga agccgtcgtc
    25021 gacgagctgc tcggcggcgt ccagcagctc caccgggtcg ggcagcagcg tgcggtcgtc
    25081 gccgatcacc tccagcttca cccagtccgt gcccagcgcc tcgcgggcga gcctggcggt
    25141 caggacggcg tcgcgcgcgg tgtggcagcc ggcggtgttg ggcagcacgc ggcagccggc
    25201 gcgctccagc acgtccagca cggagccgcg cgccgccggg tccacgcgcc gcatcgccac
    25261 cgtcgccagg tccgcgccgg aggcgcgcag cgcctgctcc agcgcgtcca ggctggccgc
    25321 gccgccgctg cccatgatga ggcgcgagcg cagcgtcacg cccgcgatca cgaacgcgtc
    25381 gccgcgtgcc gtggcctgct ccgtggcact catccgccct gcaccgccgt gaggatctcg
    25441 acctcgtcgc cgtcgtgcag ctcggccgtg tgccactgcc cgcggggcag cacggtgccg
    25501 ttcagcgcca gggccacgcc ggcgcggccc gggtccaggc ccagggcgcg cagcgcggcc
    25561 gcaggcacgg gctggtcgag ctcgcgcgcc tcgccgttga ccagcacgcg cgtcatgccg
    25621 ccaccacccg gccgaagcgg cgcgcgtcga acgcggccgc cggtccctcc aggccgccgc
    25681 cggacaggca gcccgccagc gcgtcggccg tcgcgggcgt cagcaggatg ccgttgcggt
    25741 gatgcccggt ggccgccagc aggccctcca cgcccgtggg gccgagcagc gggccgttgt
    25801 ccggcgtcgc cggccggaag ctcacgacga cgtcctcgaa cgccagctcg tccacccatg
    25861 gcaacagctc ccgcgccgcg cgcagcagct cgtgcaccgc ccccgccgtc ggcgtcgcgt
    25921 cgaagcccag ctcctccatg gtggccccga cggccacgcg cccgtcccgg cgcggcacca
    25981 cgtagacctc cacgccgcgg atgttgcgcc cggccagcgc cccggcgggc ccgcgcaggt
    26041 acacgatctg gcccttcacc ggccgcaccg gcagcagccc gtcggggatc cccgcgatgc
    26101 cagccgacca cgcgcccgcc gccagcacca cctgcggcgc ctcgacggcc gcgccgtcgg
    26161 caagctccac gccggtcacc gccgcgccgt cgcggcgcag cgccgtcacg gccgagcgga
    26221 ccagccgcac gcccgcgcgc tcgcacgccg cgagcagcgc gcgcaccagc agccgcccgt
    26281 cgacctggtg gtcgccgtgg accagcagcc cgccgcgcac cgacggcgcc aggcccggct
    26341 ccagcgcgcg gcactcgcgc gaggtgagcc ggtcggaggc caggccgagc gaggcctgca
    26401 gccggcgcag ctcctccagc gcctccaggt cgtcgcggtc gcgcgcgacc accagcgtgc
    26461 cctcggtgcg gtggccggtg tccatgccgg agacgtcctc gagctccgcg acaaacgcgg
    26521 ggtagcgcgc cgccgaggcc aggttcagcg cgagcagcgc ctcctcgccc ggatgcacct
    26581 ccgtgaccgg cgcgagcatc cccgcggccg cccaggcggc gccgcccccg ggctccgggt
    26641 cggcgaccac caccgacagc ccgcgctgcg ccgcgcgcca ggccagcgcc aggccgatgg
    26701 ggccgccgcc ggcgacggcg acgtccgcgg cgtgcgctgc ggtgggcact tctggctccc
    26761 ttcgccggca tgacccggat caggtggtga cggtcggaac cgcgcggttc cctctcagcc
    26821 cgctggactc gcggactccc gtgctggtac tcccagcata tgcgcctcgc tacagggcgg
    26881 ggtcggcgag gttcagggag gcgaggcgct gttcgagcct gtcgatctcg ccggtgacgg
    26941 cggcggcgtc gcgggcgagc ctcgcgtcga actgcgggta caggatctgg aagctggtct
    27001 cggcggcgcc gatctgccag cccatcgcct cgcagaactc ccccagctcg aactcccacg
    27061 tgaacagcag ctcgcgcccg tcgtcgtgga cccactcggt gaggtagcgg ttgggcttgc
    27121 gcgcgtactg cagcagcccc tggcggtccc gctggagcag gtggaacccc agccccgcga
    27181 gctccgcggc gaacccgtcc gatccgttcg tgccggccat ggggccgaag cctaccgtcg
    27241 cgccccgctc accgtgtccg ccggacaggg gcagccaggg aggaatgtcc ggttggcgga
    27301 catagactcg accgggatgg aacccggatc tccgaggacc gagagcctgc gcgacgtgct
    27361 ggccggccgc taccggctgg acgcgcgcgt cggggcaggc ggcatggcca cgatctaccg
    27421 cgcccgcgac ctcacgctgg accgcgacgt ggccgtcaaa gtgctgcacc cgcacctgat
    27481 ggacgacgac gggctgcggg agcgcttccg caccgaggcg cggcacgccg cccggctgct
    27541 gcaccccaac atcgtcaacg tgttcgacac cggcgaccgc gaagatcccg acgggctgcc
    27601 cgcgggctcc cccgcgaacc tgccctggat cgtcatggag tacgtggacg ggccgtccct
    27661 gcgcgacgtg ctgcgcgagc gcgagcgcct gtccccgcgc gaggcgctgt ccatcaccga
    27721 gcccgtcgcc cgggcgctcg cccgcgcgca cggcggcggc gtggtgcacc gcgacatcaa
    27781 gccggagaac gtgctgatcg ccgccgacgg cacccccaag gtcgccgact tcggcatcgc
    27841 gcgggcgctg gccgagacca gccacacgca gaccggcacg ctcatcggca gcgtgcacta
    27901 catggccccg gagctcgtca gcggcaagcc ggcgaccgcg gccaccgacc agtacggcct
    27961 cggcgtgctc ctctacgagc tgctcaccgg cacgcagccg ctgccggcgg agtcgcccat
    28021 ggcgatcgcg ctgcgccacg cccaggaggc gatcccgccg ccgagcgcgg tcaaccccga
    28081 ggtgcccgcc gccctggacg ccgtggtggc gcgggccacc gcgcacgccg ccgaggaccg
    28141 cttcccggac atggagtcct tcgccgccgc gctgcgcgcc gccgtgccgg acgggccgga
    28201 gcccgtggtg gtgcaccggc ccgtccggca cggcggcgcg cagcgcggcg gcgaaggact
    28261 ccatg
//
LOCUS       DATMKC010000289         5201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_121901, whole genome shotgun sequence.
ACCESSION   DATMKC010000289 DATMKC010000000
VERSION     DATMKC010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121901"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..772)
                     /locus_tag="VNU01_08675"
     CDS             complement(104..772)
                     /locus_tag="VNU01_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HWH32733.1"
                     /translation="MATMVKRRKRQHTVSKFYLRAFADEAGTVRRVGVPGREAVDLSV
                     NDASVIKDFYSVRLSDGSVSDAFEEAFGEVEDGAATAHRALLDGQWPLQGEARLSLAA
                     WIALQHLRTEGTRGDQEVMRATMIRLVVGSSGKEALRRHIERAESRKVGDQELDKEWA
                     DLTKPGGPDLEPDVLMHMDTVVSLLPGFAQYLRDCHWTVFRFQRRCLATSGACQIVCV
                     RAWS"
     gene            complement(916..1512)
                     /locus_tag="VNU01_08680"
     CDS             complement(916..1512)
                     /locus_tag="VNU01_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWH32734.1"
                     /translation="MTAAPDNEPGTRVLVNVLGHFDVWHGERDLTPPAGQGAAVVQLL
                     ALHDGVIHRDRLVAHLWPDDPPWVGQQRLRNVLYRLRRHTGPLVGRDPRRNNLVRYTQ
                     PVRIDLTEFLHLSAEAIRLARSDPGAAIEHGTRALMLYRGPLLLDQPDAEWAHPPRIH
                     ATERHRLLLHALAEASDRLGEPEQATLYRHRGTRSYPS"
     gene            complement(1509..1823)
                     /locus_tag="VNU01_08685"
     CDS             complement(1509..1823)
                     /locus_tag="VNU01_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32735.1"
                     /translation="MSQPNDARLEALAQRRARIIAALPSTERAVMALRDLVDSGLPGY
                     REPHGEPFAIPDGTPPGLRADLLAWEEHHQLAAQLWAEDRFKLGFALGAGLSGELFLD
                     LP"
     gene            complement(2356..3318)
                     /locus_tag="VNU01_08690"
     CDS             complement(2356..3318)
                     /locus_tag="VNU01_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32736.1"
                     /translation="MAVAHVDACEYRVVEPLAGGVAGFGVGGGAVGRERDRRVQHLPR
                     FLQVGVQRGEAILDVLGVAAEPVLFGFQCGDVDGVGVVGVEQLAALRVDLGQALGLVF
                     AFGGAALFTRGELGLESLAEAFNDAGGQLQPLVQRGDFVFDAVGGDVALTAHHVAAVF
                     LAEAEEVQVAALGMLHGQAASADAAVQQAFEVVVVLALPRASHRPGLQQRLDLLECGA
                     VDQRVVGAGVVDAVPFHDAHVDLGSCSTTARSASVPLLCLDQSSRVHAPALTRPRGDC
                     RFSHRRAGASTSFLARHAGRSMRRTQVAPDRPVGPYRIMCAARW"
     gene            complement(3392..3464)
                     /locus_tag="VNU01_08695"
     tRNA            complement(3392..3464)
                     /locus_tag="VNU01_08695"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3429..3431),aa:Ala,seq:cgc)
     gene            complement(3499..4134)
                     /locus_tag="VNU01_08700"
     CDS             complement(3499..4134)
                     /locus_tag="VNU01_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32737.1"
                     /translation="MPADLSRLRDDIDARLAAKHAAREAALPEARKAIRFAANAIRAV
                     HRGERDDAQALLAQSEAALRAAEAASADQPAVRWSGAVDDAAKEYAEGRITLALVNEE
                     PLPTPDELGVQDSAWLNGAAEAVGELRRHLLDELRAGRLERCEGLLGSMDAIHAMLVT
                     IDYPDGITGGLRRSTDVARSIIERTRGDLTTALVQARLREALEDHARNLRQ"
     gene            complement(4146..4640)
                     /locus_tag="VNU01_08705"
     CDS             complement(4146..4640)
                     /locus_tag="VNU01_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32738.1"
                     /translation="MGVAVVVLLGGLWASILVPAAVRARRESSPIHSVDLFERSLGML
                     APKTSGGPANVPGRYVMVLERPARVARAGRSSSAVERRKAILLRLAGAVPFTGLLAIL
                     GGGLFRTLFLLSVVALLGYVGLLVQVNVRRMQATQKVRRLPVRQMAPVAHERDSRAVG
                     HGRY"
     gene            complement(4731..>5201)
                     /locus_tag="VNU01_08710"
     CDS             complement(4731..>5201)
                     /locus_tag="VNU01_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWH32739.1"
                     /translation="AVITVSTRAAGGVYADDAGPAVAGRLREAGWDVADVVVVPDGRA
                     VVAQAIRDACATADVVLTSGGTGLHPKDETPEATRDVIDREVPGLAEAMRAASLAITP
                     MAMLSRAVAGVAAGTLVVNLPGSPKGAVETLEVVLPVLAHAVDQLGGGDHAARG"
BASE COUNT          823 a         1822 c         1827 g          729 t
ORIGIN      
        1 gaggcggcac cgccgccggc gtttcggaac ggtcgtgaga cgtcatctgc ttgctcatca
       61 gggtgtccct tctcgagcgc ccgcatcaag cggcgcactc gtatcaggac caggccctta
      121 cacaaacgat ctgacacgcc cccgacgtgg cgaggcaccg tcgctggaac cggaacaccg
      181 tccaatggca atcccgaagg tactgcgcga accccggcaa gagcgacacg acggtgtcca
      241 tgtgcatcag tacatccggc tctagatcgg gaccaccagg cttcgtcagg tcggcccatt
      301 ccttgtctaa ctcttggtct ccgactttgc ggctttctgc acgttcaatg tgacgacgca
      361 gcgcctcctt accggaactg ccgacgacca gccggatcat cgttgcccgc atgacctcct
      421 ggtcgccgcg cgtgccttcg gtgcgcaagt gttgcaacgc aatccaggca gccagactca
      481 gccgtgcctc gccctgcaac ggccattgac catcgaggag cgcgcggtgc gctgtagcag
      541 cgccgtcctc aacctcccca aaggcttcct cgaaggcatc cgacacggag ccgtccgaaa
      601 gccgcacgct gtagaagtcc ttgataaccg atgcgtcgtt cacggagaga tccacagcct
      661 cccgtcccgg cacaccaacc cgccggactg tgccggcctc atcagcgaag gcccgcaaat
      721 agaacttcga aacagtgtgt tgtcgcttcc ggcgcttgac catcgttgcc atgcctccag
      781 caacctcgga ataggcagcc acatagggac cgccctctga cggagccgag gaagtccccg
      841 tgtatccatc gttatacgga cccaggaagg ctctcggcga agcaagccaa gcggtggccg
      901 acggcgtaag taggcctagc tcgggtaact gcgcgtgcct cggtgacgat agagcgtcgc
      961 ctgctcgggt tctcctagcc ggtcgctggc ctcggccagg gcgtgcagaa ggaggcgatg
     1021 gcgttcggtg gcgtggatgc gtggcgggtg ggcccattcg gcgtcgggct ggtcgagcag
     1081 gagcggtcct cggtacagca tgagcgcgcg ggtgccatgc tcgatggccg cgccggggtc
     1141 gctgcgtgcg aggcggatcg cttccgcgct gaggtgcagg aactcggtga ggtcaatgcg
     1201 cacgggctgg gtgtagcgga cgaggttgtt gcggcggggg tcgcggccga cgagcgggcc
     1261 ggtgtgacgg cgcaaccggt acaggacgtt gcggagtcgt tgctggccga cccagggcgg
     1321 gtcgtcgggc cacaggtgcg cgacgaggcg gtcgcggtgg atcacgccgt cgtgcagggc
     1381 gagcagctgg acgacggcgg cgccttggcc ggcgggcggg gtgaggtcac gctcgccgtg
     1441 ccacacgtcg aagtggccga ggacgttgac cagcacccgt gtccccggct cgttgtcggg
     1501 cgcggcggtc atggcaggtc gaggaacagt tcgccggaca gtcccgcgcc gagcgcgaag
     1561 ccaagtttga agcggtcctc ggcccacagc tgggcggcga gctggtggtg ttcctcccag
     1621 gccagcaggt cggcgcgcag gccgggcggg gtgccgtcgg ggatggcgaa cggctcaccg
     1681 tgcggttcgc ggtagccggg caggccggag tcgaccaggt cgcgcagcgc catcacggcg
     1741 cgttcagttg agggcagcgc ggcgatgatg cgggcgcgtc tttgggctag ggcttccagg
     1801 cgcgcgtcgt taggctggga cacgtcaatc cctccagtgc aggggttggc cacgccccgg
     1861 gaccgttggc gcggtcgccg gggcacttcg ctgtgctgac cccgcatcgg gccgtcacag
     1921 ccggacgcga ggagcgggca ggaggccagg acgacgcggt cgtgtcaagg cacgctcaag
     1981 cactcgccac accacctgtc agaagccacc cggggttgct aaccgggtgt gcgcgacagg
     2041 cgtggacggt ccggcttgtg cgctgcgctg aggcgactgc cagcggaaat gtcttctcac
     2101 tagtgagtcg gacttctccg accccgccgg ttcggccgcc tgaccagcgg tttcgcggcc
     2161 tgccggtggc tggaaccacc gtcgccccta gcgtcgggcc tagcgtcgcc cctaacgtcg
     2221 ccccccgaag cgcgcatgac gcgggcgagc cgacggctcg cacggcgacc gtggcgaagc
     2281 gctgcggccg ttggtgcggg caggacgctt ccgggagcgt tgcccgggcg cgttgtgggc
     2341 cgcgctgggc cacgatcacc agcgggcggc gcacatgatt cggtacgggc cgacggggcg
     2401 gtctggggcg acctgggtgc ggcgcatcga gcggcctgcg tgccgcgcga ggaagctggt
     2461 ggaggcaccg gcgcgtcggt gggaaaagcg gcagtctcca cgaggacgag taagcgcagg
     2521 cgcgtggacc cgagaggatt gatcaagaca gagcagcgga acggaagctg aacgagcagt
     2581 ggtggagcaa gatccaaggt ctacatgggc gtcgtggaat gggacggcgt cgactacccc
     2641 ggctcccacg acccgctgat cgaccgcgcc acattccaac aagtccaatc gctgctggag
     2701 gcccggtcgg tgagaggcac gcgggagcgc aagcaccacc actacctcaa aggcctgctg
     2761 cactgcggcg tctgcggacg ccgcctgtcc gtgcagcatt ccaagggccg ctacctgtac
     2821 ttcttctgcc tcggccagaa aaacggcggc gacgtgatgt gccgtgagcg ctacgtcgcc
     2881 gccgaccgcg tcgaagacga agtcgccgcg ctgtacgagc ggctgcaact gcccgcccgc
     2941 gtcattgaac gcctccgcaa gggactcgag gccgagttcg ccgcgcgtga acagcgcagc
     3001 gccgccgaac gcgaatacca gtccaagcgc ctggccaaga tcgacgcgca gcgccgcaag
     3061 ctgctcgacg cctactacgc caacgccatc gacgtcgcca cactgaaagc cgaacaggac
     3121 cggctcggcc gcgacgccca gcacgtccag gatcgcctcg ccgcgctgga cgccaacctg
     3181 gaggaagcgc ggcaagtgct ggacacggcg atcgcgttcg cggccgactg cgccaccgcc
     3241 tacgccaaag ccagcgacac cacccgcaag cggttcaacg acgcggtatt cacacgcatc
     3301 gacgtgcgcg acggccacgt caccggttgg gaggcccgcc cgctctacgc agagctgttc
     3361 gacgggccgg ggttcgaata cggaagtctt gtggagctga ggggaatcga acccctggcc
     3421 tcctcgatgc gaccgaggcg ctctaccaac tgagctacag ccccgcgtgg ggacggcggc
     3481 gatggtagca ggcgccgcct actggcgcag attgcgggcg tggtcctcga gcgcctcccg
     3541 cagccgcgcc tgcaccagtg cggtggtgag atccccgcgc gtccgctcga tgatcgagcg
     3601 cgccacgtcg gtcgagcgcc gcagcccgcc ggtgatgccg tcggggtagt cgatcgtcac
     3661 gagcatcgcg tggatcgcgt ccatcgaccc cagcaggccc tcgcagcgct ccagcctccc
     3721 ggcgcgcagc tcgtccagca gatgccggcg cagctccccc accgcctcgg cggcgccgtt
     3781 cagccaggcg ctgtcctgca cgcccagctc atccggcgtg ggcagcggct cctcgttcac
     3841 cagcgccagc gtgatgcgcc cctcggcgta ctccttggcg gcgtcgtcca ccgccccgga
     3901 ccagcgcacc gccggctggt cggcgctggc cgcctcggcc gcgcgcagcg ccgcctcgga
     3961 ctgcgcgagc agcgcctggg cgtcgtcgcg ttcgccgcgg tggaccgcgc ggatcgcgtt
     4021 ggcggcgaag cggatggcct tgcgcgcctc gggcagcgcg gcctcgcggg cggcgtgctt
     4081 ggcggccagc cgggcgtcga tgtcgtcgcg caggcgcgac aggtcggcgg gcatcgcggc
     4141 ggggctcagt accgcccgtg gccgacggcg cgcgagtcgc gctcgtgggc gaccggcgcc
     4201 atctgccgca ccggcaggcg gcgaaccttc tgcgttgcct gcatgcgccg cacgttgacc
     4261 tgcaccagca ggccgacgta gccgaggagg gcgaccacgc tcagcaggaa cagcgtccgg
     4321 aagaggccgc cgcccaggat cgcgagcagc cccgtgaacg gcacggcgcc cgccaggcgc
     4381 agcaggatcg ccttgcgccg ctccacggcg gaggaggagc gcccggcgcg ggcgacccgc
     4441 gcgggacgtt cgaggaccat cacatagcgt cccggcacgt tggccggccc accggaggtc
     4501 tttggtgcga gcatgcccaa gctgcgctcg aagaggtcga cggaatggat gggggatgac
     4561 tcacggcgcg cgcgcaccgc agcaggcacg agtatcgagg cccacagacc accgagcaat
     4621 acgacgaccg ctacgcccat cgagtccgca ccgtaacacc acgatgaatg gattcggtgg
     4681 aaccggtgac tgccaccggg ccccgtaccc acgcgctcgt tcagaggcta tcagccccgc
     4741 gcggcgtgat cacctccgcc gagctggtcc accgcgtgcg cgagcacggg cagcaccacc
     4801 tccagcgtct ccacggcgcc cttgggcgag ccgggcaggt tcaccaccag cgtgccggcg
     4861 gcgacccccg ccacggcccg cgacagcatc gccatcggcg tgatcgcaag ggacgccgcg
     4921 cgcatcgcct ccgccaggcc gggcacctcc cggtcgatca cgtcgcgcgt ggcctccggc
     4981 gtctcgtcct tggggtgcag ccccgtgccg ccggaggtca gcaccacgtc cgccgtcgcg
     5041 cacgcgtcgc ggatcgcctg cgccacgacg gcgcggccgt ccggcaccac caccacgtcg
     5101 gccacgtccc agccggcctc gcgcagccgc ccggcgacgg cgggcccggc gtcgtcggcg
     5161 tagacgccgc ccgccgcgcg cgtcgacacg gtgatgacgg c
//
LOCUS       DATMKC010000290         4896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_314185, whole genome shotgun sequence.
ACCESSION   DATMKC010000290 DATMKC010000000
VERSION     DATMKC010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314185"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="VNU01_08715"
     CDS             complement(<1..1695)
                     /locus_tag="VNU01_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH32740.1"
                     /translation="MTSMFPHHRVLAVRCAALLAFLLTAMTAAAPAAAGQEASGGAPG
                     GHAPVATHDGGDALPGRLLVTLDADVALGSTVAPFGGSAEPISDSVHLVRVPPARRGE
                     AELALRRLPGVRAVEPDRLRPWARVPDDAQFAEQWSHTMTLAPRAWDTTVGSRDVRVA
                     LIDSGVRGNHAELAGNVLEQVDLSSGAPVPRARGVDNDTCEIGHGTQVAGIIGAAGDN
                     GMGVAGVAWEVGIIDLAVSALATPGSCDGASDSAILAALHYATYHPSGPVDVVNLSVG
                     GRQAFCPEAYERAIDEARREGVVVIAAAGNSGPETAQVPASCPGVIAVGAVDVDGRVP
                     SYSATNPWVDLVAPGGGDSTSHMLLTTSRRGDWTRVRGTSFAAPYVSGVVALMRAVNP
                     LLSPDEVESVLERSAHDGGLGRTDAQGWGRVHAARALSWVRRDLSIPPPEAEPYFPVL
                     RVHSLAPGPGPERLGSHDGATDPVLQAAQVSRAVFREAGAVHAVVARADDFADALAGS
                     ALGMGVGPLLFNAPGQELSPQVRKELRRALPAGARVYVLGGERGLGRGVADDLRGMG"
     gene            complement(1748..2569)
                     /locus_tag="VNU01_08720"
     CDS             complement(1748..2569)
                     /locus_tag="VNU01_08720"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HWH32741.1"
                     /translation="MTAPRRPGHPDPLSPGEKVLLVDRKERRYFVVLEPGKDWHFHGG
                     IVHHDDILGRAEGSRVRSSKGSTLLVLRPTSGDWTVKAPRGAQVVYPKDQAMIVALAD
                     VMPGVTVAEAGAGSGALTSALLRAVGPEGRVISFELRDDHADIAERNVIERFGGRPAN
                     WELRRGDVVEGLAGVRCERVVLDLLEPWEVLKAAAEALHPGGIVLAYTPTVPQVMRLH
                     EAFTADARWGFAKTVETLVRGWHVDGLAVRPDHRMVAHTAFLTTARRLPDEPVEA"
     gene            complement(2566..3723)
                     /locus_tag="VNU01_08725"
     CDS             complement(2566..3723)
                     /locus_tag="VNU01_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32742.1"
                     /translation="MKVKLRNPERLVDVPGPVSVHQMLATLDINPETVLVIHDGTLVT
                     KDHVLPDDARVEVRPVISGGAGPAKCTHCKAPSIIEIARHRAAYCADCFTDHVRAQVR
                     DAVRRYDMMSYDDRILVAVSGGKDSLALWDVLLDMGYRADGMYLGLGIGSYSDNSEKI
                     VRDYAEARGATLHLVDLAGEYGYDIPQATGLKGRSSCGVCGLSKRYAFNKVALEHGYD
                     VMATGHNLDDEAAQLLGNVLRWQTPFMARQSPCLPRSETMAKKVKPLYRLGEREMAAY
                     CVIKGIDYVVEECPMVEGNTVLRYKDALNELERHSPGTKGQFLFGFLERARPQLFPDG
                     DPEGHRLVDCAECGMPTTTPPRQEAAGTEPVLCAFCRTRGRILQVVQKAAR"
     gene            complement(3785..4645)
                     /locus_tag="VNU01_08730"
     CDS             complement(3785..4645)
                     /locus_tag="VNU01_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH32743.1"
                     /translation="MTATIDPAEFHEAQRQMWDGAADAWRRWWPVIEEAARPVTDRLV
                     SLAEVAPGDQVLDVGTGIGEPALTAARACAPGGRVLATDLSGQMLQIARDRAQDAGLD
                     NVEFREAAGDQLGLDGEFDVALSRFTLMLVAEPDAMLASMRRALRPGGRMACAVWSSP
                     DRVPMIAVPMLTLLRELQVEPPPPGTPGIFSLADPARLEGLVREAGFTDVAVEAVVGQ
                     VTFPDAEEFARYIGEVAGPGIQLALASLPEARRIEAWDAVVDALRAHAGEDGSVTLSA
                     EALCVHGRAG"
BASE COUNT          610 a         1848 c         1791 g          647 t
ORIGIN      
        1 gcccatgccg cgcaggtcgt cggccacgcc ccggcccagc ccgcgctcgc cgcccaggac
       61 gtacacgcgc gccccggcgg gcagggcgcg gcgcagctcc ttgcgcacct gcggcgacag
      121 ctcctggccg ggcgcgttga acagcagggg cccgacgccc atgcccagcg cgctgccggc
      181 cagggcgtcg gcgaagtcgt cggcgcgcgc gaccacggcg tgcaccgcac cggcttcgcg
      241 gaacaccgcg cgcgacacct gcgccgcctg cagcaccggg tcggtggccc cgtcgtgcga
      301 gcccagccgc tccggccccg gtcccggggc gaggctgtgg acgcgcagca cggggaagta
      361 cggctcggcc tccggcggcg ggatgctcaa gtcccggcgc acccagctca gcgcccgcgc
      421 ggcgtgcacg cgcccccagc cctgcgcgtc ggtgcgcccc aggccgccgt cgtgcgccga
      481 gcgctccagg accgactcca cctcgtctgg cgagagcagc gggttgaccg cgcgcatgag
      541 ggcgacgacg ccgctgacgt agggggcggc gaagctggtg ccgcgcacgc gggtccagtc
      601 gccgcggcgg gacgtggtga gcagcatgtg gctggtgctg tcgccgccgc cgggggcgac
      661 caggtccacc cacgggttcg tggcgctgta ggacggcacg cggccgtcga cgtcgacggc
      721 gccgaccgcg atgacgccgg ggcaggaggc cgggacctgg gcggtctccg ggccgctgtt
      781 gcccgcggcg gcgatgacca cgacgccttc gcggcgcgct tcgtcgatgg cgcgctcgta
      841 ggcctccggg cagaacgcct ggcggccgcc gacggacagg ttgaccacgt ccaccgggcc
      901 gctcgggtgg tacgtcgcgt agtgcagcgc ggccaggatc gcgctgtcgc tcgcgccgtc
      961 gcagcttccc ggcgtggcca gcgcgctgac cgccaggtcg atgatgccga cctcccaggc
     1021 caccccggcc acgcccatgc cgttgtcccc ggccgcgccg atgatgcccg cgacctgcgt
     1081 gccgtggccg atctcgcagg tgtcgttgtc gacgccccgc gcgcgcggga ccggggcgcc
     1141 gctggacagg tccacctgct ccaggacgtt gccggcgagc tcggcgtggt tgccgcgcac
     1201 gccgctgtcg atcagcgcga cgcgcacgtc ccggctgccg accgtggtgt cccacgcgcg
     1261 cggcgccagc gtcatggtgt ggctccactg ctcagcgaac tgcgcgtcgt ccggcacgcg
     1321 cgcccacggg cgcaggcggt cgggctccac ggcgcgcacg ccgggcaggc ggcgcagcgc
     1381 cagctcggcc tcgccgcggc gcgccggcgg gacgcgcacc aggtgcacgc tgtcgctgat
     1441 cggctcggcg gagccgccga acggcgccac cgtcgagccg agcgccacgt cggcgtccag
     1501 ggtgaccagg agcctgcccg gcagcgcgtc gccaccgtcg tgggtcgcga cgggagcgtg
     1561 cccgccgggt gccccgccgg aggcttcctg gcccgcggcg gcgggcgcgg cggccgtcat
     1621 ggccgtgagc aggaacgcca gcagcgccgc gcagcgcacg gcaaggacac ggtggtgcgg
     1681 gaacatcgac gtcatgcagc gcttctcttc ggcggacgtg gcttggacgc cccggatgcc
     1741 ggactcgtta cgcctcgacc ggctcgtccg gaagtcttcg cgccgtggtg aggaacgccg
     1801 tgtgcgcgac catgcggtgg tcgggccgca cggccagccc gtcgacgtgc cagccgcgca
     1861 cgagcgtctc gacggtcttg gcgaagcccc agcgcgcgtc cgcggtgaac gcctcgtgca
     1921 ggcgcatcac ctgcggcacg gtgggcgtgt acgccagcac gatcccgccg gggtgcagcg
     1981 cctcggcggc ggccttgagg acctcccacg gctccagcag gtccaggacc acgcgctcgc
     2041 agcgcacgcc ggccaggccc tcgacgacgt cgccgcggcg cagctcccag ttggccggcc
     2101 gcccgccgaa gcgctcgatc acgttgcgct cggcgatgtc ggcgtggtcg tcgcgcagct
     2161 cgaagctgat cacgcgcccc tccggcccca ccgcgcgcag cagcgcgctg gtgagcgcgc
     2221 cggacccggc gccggcctcc gccacggtga cgccgggcat cacgtcggcc agcgccacga
     2281 tcatcgcctg gtccttgggg tagaccacct gcgcgccgcg cggcgccttg accgtccagt
     2341 ccccggaggt gggccgcagg accagcaggg tgctgccctt gctgctgcgg accctgctgc
     2401 cctcggcacg gcccaggatg tcgtcgtggt ggacgatgcc gccgtggaag tgccagtcct
     2461 tgccgggctc cagcaccacg aagtagcggc gctccttgcg gtcgaccagc agcaccttct
     2521 caccgggcga cagcgggtcg ggatggccgg gcctgcgcgg ggcggtcacc gcgcggcctt
     2581 ctgcacgacc tgcaggatgc gcccgcgcgt ccggcagaac gcgcacagga ccggctcggt
     2641 acccgcggct tcctggcgcg gcggggtggt ggtgggcatg ccgcactcgg cgcagtcgac
     2701 caggcggtgg ccctccgggt cgccgtccgg gaacagttgc gggcgcgcgc gctccaggaa
     2761 gccgaacagg aactggccct tggtgccggg cgagtggcgc tccagctcgt tcagcgcgtc
     2821 cttgtagcgc agcaccgtgt tgccctccac catcgggcac tcctcgacca cgtagtcgat
     2881 gcccttgatg acgcagtagg cggccatctc ccgctcgccc aggcggtaca gcggcttgac
     2941 cttcttcgcc atggtctcgc tccgcggcag gcacggggac tggcgcgcca tgaacggcgt
     3001 ctgccagcgc agcacgttgc cgagcagctg cgccgcctcg tcgtccaggt tgtggcccgt
     3061 cgccatgacg tcgtagccgt gctcgagcgc gaccttgttg aaggcgtagc gcttggacag
     3121 cccgcacacc ccgcaggagg agcggccctt gaggccggtg gcctgcggga tgtcgtagcc
     3181 gtactccccc gccaggtcca ccaggtgcag cgtggcgccg cgcgcctcgg cgtagtcccg
     3241 cacgatcttc tcggagttgt ccgagtagct gccgatgccc aggcccaggt acatgccgtc
     3301 cgcgcggtag cccatgtcca gcagcacgtc ccacagcgcc agcgagtcct tgcctccgct
     3361 gaccgcgacg aggatgcggt cgtcgtagct catcatgtcg tagcggcgga ccgcgtcgcg
     3421 gacctgcgcg cgcacgtggt cggtgaagca gtcggcgcag taggcggcgc ggtggcgcgc
     3481 gatctcgatg atggacggcg ccttgcagtg cgtgcacttg gcgggcccgg cgccgccgga
     3541 gatcaccggc cgcacctcca cccgcgcgtc gtcgggcagc acgtggtcct tggtcaccag
     3601 cgtgccgtcg tggatcacca gcaccgtctc cgggttgatg tccagggtcg ccagcatctg
     3661 gtgcaccgac acggggccgg gcacgtcgac gaggcgttcg gggttgcgca gcttgacctt
     3721 catggcgccc tgaaggctag cgcgcgcgaa gccccgctcc cccgctgggc cggtccgggt
     3781 cgtgtcagcc cgcccggccg tgcacgcaca gcgcctcggc ggacagcgtg acggacccgt
     3841 cctcgccggc gtgtgcgcgc agcgcgtcga cgacggcgtc ccacgcctcg atgcgacgcg
     3901 cctccggcag gctcgcgagc gcgagctgga tgcccgggcc cgccacctca ccgatgtagc
     3961 gggcgaactc ctcggcgtcg gggaacgtga cctggccgac caccgcttcc acggcgacgt
     4021 cggtgaagcc ggcctcgcgc acgaggccct ccaggcgcgc cgggtcggca agcgagaaga
     4081 tcccgggtgt gcccggcggc ggcggctcca cctgcagctc gcgcagcagg gtcaacatgg
     4141 ggacggcgat catcgggacg cggtcgggcg aactccacac cgcacaggcc atgcggccgc
     4201 cggggcgaag cgcccggcgc atggacgcga gcatcgcgtc aggctccgcg acgagcatga
     4261 gcgtgaagcg ggacagggcg acgtcgaact cgccgtccag cccgagctgg tcccccgcgg
     4321 cctcgcggaa ctccacgttg tccaacccgg cgtcctgcgc gcggtcacgg gcgatctgca
     4381 gcatctgccc ggacaggtcc gtcgccagca cgcgcccgcc tggcgcgcag gcacgcgcgg
     4441 cggtcagcgc cggttcgccg atgcccgtgc cgacgtcgag cacctgatcc ccgggcgcga
     4501 cctccgccag cgagaccagc cggtcggtca ccggccgggc ggcctcctcg atgaccggcc
     4561 accagcgccg ccatgcgtcg gctgcgccat cccacatctg gcgttgcgcc tcgtgaaact
     4621 ccgccggatc gatcgtggcc gtcatggtgt ccaccccctg gattgtcccg gccggcgccg
     4681 ccaccggcag gccgtcgcgg cctgagccga tcctacggtg tgtgcgggcc cgcgtgtgcg
     4741 gcggggccgg gcggcccggg gtgtgtcgac tgggtcatcc tgacattggt gcagtcgaca
     4801 cacccccggt ttctgcaggc gaacgcctag gcttgggatc atgagcacga tgcgggcact
     4861 gcaactgcag ctccagccgc tgcgggcggc ctacgc
//
LOCUS       DATMKC010000291         7674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_269238, whole genome shotgun sequence.
ACCESSION   DATMKC010000291 DATMKC010000000
VERSION     DATMKC010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269238"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="tilS"
                     /locus_tag="VNU01_08735"
     CDS             complement(<1..904)
                     /gene="tilS"
                     /locus_tag="VNU01_08735"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWH32744.1"
                     /translation="MSALGPDGALPPGASAERLAGEARRVLDACLEPGARVLLAVSGG
                     ADSVALALLVRRARPDLDAAVLHVRHGLRDDAADAACAAEAADRLGLPFRAVEVTVDL
                     RSGASGPEAAARSARYAAFRRTCREAGARAVLAGHTADDQAETVLLNLARGAGLPGAA
                     GMAADRTDESGLRLVRPLLRMRRADVRAVLPEAGLRWVEDPTNLDPSQRRARARREAL
                     PALARLTGEGGDVVGALTRFAAHAGRESHALDVLAERELGRVGRRWGAVVALPVARLA
                     AMPEGLALRVLRAAVADLGVAALPA"
     gene            complement(901..2052)
                     /locus_tag="VNU01_08740"
     CDS             complement(901..2052)
                     /locus_tag="VNU01_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HWH32745.1"
                     /translation="MARDPRATLADWDLAARLAWSVASGPLAPRQPADRRTVAALRRD
                     LDATVATADGLARRAAGLGEHLPPARAHVMGRRAWIRANLDALAELTDPLADKLLRRR
                     ARGQRALARQGMALQIGAVFGYLSTRVLGQYEVFGPGWRDGSPGDGGGGRLILVGPNL
                     LELEASHLPGSGVSVAEFRLGVCLHEIAHRLQFEAVPWARPLLRGLLEEYLDDVDFDP
                     ERLREIAGRLPELLRDPARLLEPRGLLELLLTQRQVEVIGRAQALMSLFEGHGNVVMD
                     WGAELAATEGRDAPDPARVREVLNRRRGQALNQVVQKAIGLSMKAEQYRAGEAFILTV
                     AERHGRDVFNRVWASSANVPTPDEIDDPDAWAARVVAAPAAGTGGAGTA"
     gene            complement(2073..2393)
                     /locus_tag="VNU01_08745"
     CDS             complement(2073..2393)
                     /locus_tag="VNU01_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32746.1"
                     /translation="MSKRLLGVVLLAVLAVAGLAGPVAAVEEGDHGPVRHELPSIDEV
                     GSQSDTAREFFPPEYEQPPVFPWFGWPLLIGGLVLTLWVMLSYLVFQPRFAKERKAKA
                     ARKR"
     gene            complement(2551..2826)
                     /locus_tag="VNU01_08750"
     CDS             complement(2551..2826)
                     /locus_tag="VNU01_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32747.1"
                     /translation="MARQPGSSEPDDGDARAEGAARIARAAAAGRNRLLSDANLIDAL
                     VRIDEIEVATRAIAEQRADLVEYAIDLREAVAEAVQTRDAADQEAAE"
     gene            3071..3799
                     /locus_tag="VNU01_08755"
     CDS             3071..3799
                     /locus_tag="VNU01_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HWH32748.1"
                     /translation="MYSTAQKALAEFVGTFALIFVGVGAIFVTTLDGSGANLTTVALG
                     HGLAIAVMVSALAHVSGAHFNPAVTAAALATRKISVREGAVYLAAQLAGGTAAAAIAM
                     AALPGGGDAVAGGTPVLNLDQISVGQGVLIEIVLTFFLVWVIFGTAMDPRGAFRQIAG
                     MAIGFTVLLDITMGGPLTGAAMNPARAFGPALVSGTWDHHWVYWVAPVIGGLLAGLLY
                     DLVILRGREEAGPGGAAAAADADG"
     gene            3859..4545
                     /locus_tag="VNU01_08760"
     CDS             3859..4545
                     /locus_tag="VNU01_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32749.1"
                     /translation="MRPVDLSVSEAVRVPSGGATLDGDLVVPSDASGIVLFAHGSGSG
                     RRSPRNRMVAEALHVAGLGTLLVDLLTAEEALADARGARNRFDIPLLAARLADAAAWL
                     AGDERTAALPAGCFGASTGAAAALVAAAAAPDRFGAVVSRGGRPDLAGDHLEAVRTPV
                     LLLVGGQDTQVLQLNREAAARLAGEHELVVIPGAGHLFEEPGALEQVSSLAAKWFLRR
                     LPQTATRSER"
     gene            complement(4518..5693)
                     /locus_tag="VNU01_08765"
     CDS             complement(4518..5693)
                     /locus_tag="VNU01_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWH32750.1"
                     /translation="MVPFDPADPAFVRDPYPVYARLRREPGLVRHERGGMWLVARHAE
                     VDAVFRDRRLGRVFEPRTPQDFYAPWNLVNVHSMLEMEPPDHTRLRGLVAREFTPRRV
                     EALRAHIGGLVDALLDPIAERGGGDLVAELAEPLPVAVIAELLGFPDSDRHLLRPWSG
                     DIVSLYELEPQRGADERAVRAAREFGGYLRELIAARRREPGPDLFSALVQLTDEGDRL
                     TEDELVATAVLLLNAGHEASVNVMANGVLALLRHPEQMARLAADPSLAPTAVEELIRY
                     DSPLSLFTRTALEDVEVGGQALAAGEVVGLLLGAANRDPAAFAEPDRVDVGRKPNNHV
                     GFGAGIHYCLGAPLARVELQEGLARLLGRFPRMALAEEPQPRPTFQFRGYRSLRVAV"
     gene            5823..6404
                     /locus_tag="VNU01_08770"
     CDS             5823..6404
                     /locus_tag="VNU01_08770"
                     /inference="COORDINATES: protein motif:HMM:NF037470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HWH32751.1"
                     /translation="MGGRRVIGYDATVGAAVGLWLRTGTIDMDALARRMAVSRATLYR
                     VVPGRDRLLGDVLWRQAGVLFARARRDARGAGADRVLDVLRRFGEDIVSAQPFRQFIR
                     AEPDTAIRVLFTPAGAVHERFVALNRDLLAEEAAAGTLVPPFDLDDLAYVFVRVYESM
                     WYADLLAGREPNLRVADCAARAMLRAATPPADG"
     gene            6538..7266
                     /locus_tag="VNU01_08775"
     CDS             6538..7266
                     /locus_tag="VNU01_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32752.1"
                     /translation="MLRRSWGLLAVVMALAAVAFAAPAAATHRPNHGGGGDPSPSPSP
                     TANGEDPILFVHGWNSSGTVWTTMIDRFKADGYTDGQLFNWSYNYRQSNEITAQEVLA
                     KIQDIKTQTGAAKVDVITHSMGGLSSRWCVKFTGCDGSVDGWVSLGGPNHGTDTANFC
                     FDTSCTEMRIGSDFLTTLNAGDETPGSATRWRTWWSPCDSTINPDSSVSLSGATNTKT
                     ACLSHSALYEDATVYGQVRDFVGA"
     gene            complement(7383..>7674)
                     /locus_tag="VNU01_08780"
     CDS             complement(7383..>7674)
                     /locus_tag="VNU01_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HWH32753.1"
                     /translation="VATMPSEADLRSEEVGPLVERLLAGRADVPTEARMRIIRLIENL
                     TLGRNAVGYLTESLHGAGSPQAQRIQIGRGMDIEAKKALARELAGILADSAG"
BASE COUNT          955 a         2955 c         2826 g          938 t
ORIGIN      
        1 ccgccggcag cgccgccacg ccgaggtcgg ccacggccgc acgcagcacg cgcagcgcga
       61 ggccttccgg cattgccgcc aggcgggcga ccggcagcgc gaccaccgcg ccccagcggc
      121 gcccgacacg gcccagctcg cgctccgcca gcacgtccag cgcgtggctc tcccgcccgg
      181 cgtgggcggc gaagcgcgtg agcgcgccga ccacgtcgcc gccctcgccg gtcagccgcg
      241 ccagcgcggg cagcgcctcg cggcgggccc gggcgcgccg ctgggacggg tcgagattgg
      301 tcgggtcctc cacccagcgc agcccggcct ccggcagcac ggcgcgcacg tccgcccggc
      361 gcatccgcag cagcgggcgc accagccgca ggccggactc gtcggtgcgg tcggcggcca
      421 tgccggccgc cccgggcagg cccgcgccgc gggccaggtt gagcagcacg gtctcggcct
      481 ggtcgtcggc ggtgtggccg gccagcacgg cgcgcgcgcc cgcctcacgg caggtccggc
      541 ggaacgcggc gtagcgggcc gaccgcgcgg ccgcctcggg cccggacgcg ccggagcgca
      601 gatcgacggt cacttccacg gcgcggaacg gcaggccgag ccggtccgcc gcctcggcgg
      661 cgcaggcggc gtccgcggcg tcgtcgcgca ggccgtgccg cacgtgcagg acggcggcgt
      721 ccaggtccgg ccgggcgcgg cgcaccagca gcgccagcgc gaccgagtcg gcgccaccgg
      781 aaacggccag cagcacgcgc gcgcccggct ccaggcaggc gtccagcacc cgccgcgcct
      841 cgccggccag gcgttccgca gacgcgcccg gcggcagcgc gccgtccggt cccagtgcgc
      901 tcacgccgtc ccggctccgc ccgtcccggc ggcgggggcg gccacgaccc gcgccgccca
      961 ggcatccgga tcgtcgatct catcgggcgt gggcacgttg gcggaggacg cccacacgcg
     1021 gttgaacacg tcccgtccgt ggcgctcggc gacggtcagg atgaacgcct cgcccgcccg
     1081 gtactgctcg gccttcatgg acagcccgat cgccttctgc accacctggt tgagtgcctg
     1141 cccgcggcgg cggttgagga cctcccgcac gcgcgccggg tcgggcgcgt cccggccctc
     1201 cgtggcggcc agctccgcgc cccagtccat caccacgttg ccgtgcccct cgaacagcga
     1261 catgagcgcc tgcgcgcggc cgatgacctc cacctggcgc tgggtcagca gcagctccag
     1321 caggccccgg ggctccagca ggcgcgcggg gtcgcgcagc agctccggca ggcgcccggc
     1381 gatctcccgc aagcgctccg ggtcgaagtc gacatcgtcc aggtactcct ccaacaggcc
     1441 gcgcagcagc ggccgggccc acggcaccgc ctcgaactgc agccggtggg cgatctcgtg
     1501 caggcacacg cccaggcgga actccgcgac gctcacgccg gagccgggca ggtgggacgc
     1561 ctccagctcc agcaggttgg ggccgaccag gatcaggcgc cccccgcccc cgtcgccggg
     1621 cgagccgtcg cgccagccgg gcccgaacac ctcgtactgg cccagcacgc gcgtggacag
     1681 gtagccgaac accgcgccga tctgcagggc catgccctgg cgcgcgaggg cgcgctggcc
     1741 gcgggcgcgg cggcgcagca gcttgtccgc cagcgggtcg gtgagctccg ccagggcgtc
     1801 gaggttcgcg cggatccagg cacgccggcc catgacgtgc gcgcgcgccg gcggcaggtg
     1861 ctcgcccagg ccggccgcgc ggcgggccag gccgtccgcg gtggcgacgg tggcgtccag
     1921 gtcgcggcgc agcgcggcga ccgtccggcg gtccgcgggc tgacggggcg ccagggggcc
     1981 ggacgcgacc gaccaggcga gccgtgccgc gaggtcccag tcagccagtg tcgctcgcgg
     2041 gtccctcgcc actggagcgc ctcctgtcgc cgctacctct tgcgcgcggc cttggccttg
     2101 cgctccttgg cgaagcgcgg ctggaagacc aggtaggaca gcattaccca cagcgtcagc
     2161 accaacccgc cgatcagcag gggccagccg aaccagggga acaccggcgg ctgctcgtac
     2221 tccggcggga agaactcacg cgccgtgtcc gactgggagc cgacctcgtc gatggagggc
     2281 agctcgtggc ggaccggccc gtggtcgccc tcctccaccg ccgcgaccgg accggccagg
     2341 ccggcgacag cgagcaccgc cagcaggacg acgccgagca ggcgcttgga catgcggacc
     2401 tccgtgaaca gccggaccgg tacggcgggc agtgtagcgg cagtgccgcc gagcgaccca
     2461 accgctggac gggacggggt catcctcgct cggcgggaac ccctcttcag cgctcgctcg
     2521 gacgaccccg tcccgtcctg cgagccgagt tcattcggcc gcttcctggt cggctgcgtc
     2581 gcgcgtctgc accgcctcgg cgaccgcctc gcgcagatcg atggcgtact ccaccaggtc
     2641 cgcgcgctgc tcggcgatcg cgcgcgtggc cacctcgatc tcgtcgatgc gcaccagcgc
     2701 gtcgatcagg ttggcgtccg acagcagccg gttgcggccg gcggccgcgg cgcgcgcgat
     2761 gcgcgcggcg ccctcggccc gcgcgtcgcc gtcgtcgggc tccgacgagc ccggttgcct
     2821 agccatgcct ggtccgccat tcctcgcgcg acgttcatgg cgcgccgcac tcgctgcccg
     2881 actcaattcg ccgcgtccgg cacctacccc tgcgaacgcg tgcgaatcct cacactttcg
     2941 gcccgcgccc cggcggaggc ggcgcaccgt cgctcgcgcc gtcgcggtgt agcttccttg
     3001 gccgatccgg tccgcggcgt cacggcgcgg gcgagcaccc gaccctggca ggcgtccttg
     3061 aggagggacc atgtactcga ccgcgcagaa ggccttggcg gagttcgtcg gcacgttcgc
     3121 gctgatcttc gtgggcgtgg gcgcgatctt cgtcacgacc ctggacggca gcggggccaa
     3181 tctcacgacc gtggcgctgg gccacggcct ggcgatcgcc gtcatggtga gcgccctggc
     3241 gcacgtgtcc ggcgcgcact tcaaccccgc ggtcaccgcg gccgcgctgg ccacgcgcaa
     3301 gatctccgtg cgtgagggcg cggtgtacct ggccgcccag ctcgccggcg gcacggccgc
     3361 ggcggcgatc gccatggcgg cgctgcccgg cggcggcgac gccgtggccg gcggcacgcc
     3421 ggtgctgaac ctggaccaga tctccgtggg ccagggcgtg ctgatcgaga tcgtgctgac
     3481 gttcttcctg gtctgggtga tcttcggcac cgccatggac ccgcgcggcg cgttccgcca
     3541 gatcgccggc atggcgatcg gcttcacggt gctgctggac atcaccatgg gcgggccgct
     3601 gaccggcgcg gcgatgaacc cggcacgcgc cttcggcccc gcgctggtct ccggcacctg
     3661 ggaccaccac tgggtctact gggtcgcgcc ggtcatcggc ggcctgctgg ccggcctgct
     3721 gtacgacctg gtgatcctgc gcggccgcga ggaggccggc cccggcggcg cggccgcggc
     3781 cgccgacgcc gacggctagc ccgcgaccgc gcggcggctc gcgcgtgggg tcggcggccc
     3841 gcgggtacgc ccggccccat gcgacctgtc gatctcagcg tgtccgaggc cgtccgcgtc
     3901 ccgtcgggcg gcgccacctt ggacggcgac ctcgtcgtgc cgtcggatgc ctccggcatc
     3961 gtgctgttcg cccacggcag cggctcgggg cggcgcagcc cgcgcaaccg catggtggcc
     4021 gaggcgctcc acgtcgccgg gctcggcacg ctgctggtgg acctgctcac cgccgaggag
     4081 gcgcttgccg acgcgcgcgg cgcccgcaac cgcttcgaca tcccgctgct ggccgcgcgc
     4141 ctcgcggacg cggccgcctg gctggccggc gacgagcgca ccgccgcgct gcccgccggc
     4201 tgcttcgggg ccagcacggg cgccgccgcc gcgctggtcg cggccgccgc ggcgccggac
     4261 cgcttcggcg cggtggtgtc gaggggcggc aggcccgatc tggcgggcga ccacctggag
     4321 gcggtgcgga ccccggtgct gctgctggtg ggaggacagg acacgcaggt cctccagctc
     4381 aatcgcgagg ccgccgcccg cctggccggc gagcacgagc tcgtggtgat ccccggcgcc
     4441 ggccacctgt tcgaggagcc cggcgccctg gagcaggtgt ccagcctggc ggcgaagtgg
     4501 ttcctccgtc gcctgcctca gaccgccacg cgcagcgagc ggtagccgcg gaactggaac
     4561 gtggggcgcg gctggggctc ctcggccagc gccatgcgcg ggaagcgccc cagcagccgc
     4621 gccagcccct cctgcagctc cacgcgcgcc agcggcgcgc ccaggcagta gtggatcccc
     4681 gcgccgaagc ccacgtggtt gttgggcttg cgtcccacgt ccacgcggtc gggctcggcg
     4741 aaggccgccg ggtcccgatt cgccgcaccg agcagcaggc ccacgacctc gcccgcggcc
     4801 agcgcctgac cgcccacctc gacgtcctcc agggccgtgc gcgtgaacag cgacagcggc
     4861 gagtcgtagc ggatcagctc ctccaccgcg gtgggcgcga gcgagggatc ggccgccagg
     4921 cgcgccatct gctcggggtg gcgcagcagc gccagcacgc cgttggccat gacgttcacg
     4981 gaagcctcgt ggccggcgtt gagcagcagc accgccgtgg ccaccagctc gtcctcggtc
     5041 aggcggtcac cctcgtcggt gagctggacc agggcgctga acaggtccgg ccctggttcc
     5101 cgccgacgcg ccgcgatcag ctcgcgcagg tagccgccga actcccgcgc cgcgcgcacg
     5161 gcgcgctcgt ccgcgccccg ctggggctcc agctcgtaca gcgagacgat gtcgccggac
     5221 cacgggcgca gcaggtggcg gtccgagtcc gggaagccga gcagctcggc gatcaccgcc
     5281 acgggcagcg gctcggccag ctccgcgacc aggtcgccgc cgccgcgctc cgcgatgggg
     5341 tccagcaggg cgtccaccag gccgccgatg tgcgcgcgca gcgcctccac ccggcgcggc
     5401 gtgaactctc tggccaccag cccgcgcagg cgggtgtggt cgggcggctc catctccagc
     5461 atcgagtgga cgttgaccag gttccatggc gcgtagaagt cctgcggcgt gcgcggctcg
     5521 aacacgcggc ccaggcgacg atcgcggaag acggcgtcca cctcggcgtg gcgggccacc
     5581 agccacatgc cgccgcgctc gtggcgcacc agccccggct cccgccgcag ccgcgcgtac
     5641 accgggtagg ggtcgcggac gaacgcgggg tctgcggggt cgaacgggac catccgggat
     5701 tcctcgggca tgcggcggac cgggtcttcc cgcaaggcta cgtggcccgt agcgtgtgcg
     5761 cgccagggtc ggccgcgggg acggccggag gggacgaagt ggggagcagc atcgccgggc
     5821 ccgtgggagg ccggcgcgtc atcggctacg acgccacggt gggcgccgcc gtgggcctgt
     5881 ggctgcgcac ggggaccatc gacatggacg cgctcgcgcg ccgcatggcg gtcagccgcg
     5941 ccacgctcta ccgggtcgtg cccggccgcg accggctgct cggcgacgtg ctgtggcgcc
     6001 aggcaggcgt gctgttcgcc cgtgcccgcc gggacgcccg gggggcaggc gcggaccgcg
     6061 tgctggacgt cctgcgccgc ttcggcgagg acatcgtgag cgcgcagccg ttccgccagt
     6121 tcatccgcgc cgagcccgac accgccatcc gagtgctgtt cacgcccgcg ggcgccgtgc
     6181 acgagcgctt cgtggcgctg aaccgggacc ttctggcgga ggaggcggca gccggcacgc
     6241 tggtcccgcc gttcgatctc gacgacctgg cctacgtctt cgtccgcgtc tacgagtcca
     6301 tgtggtacgc cgacctgctc gccgggcgag aaccgaacct gcgcgtcgcc gactgcgcgg
     6361 cgcgggccat gctgcgggcc gccacgccac cggccgacgg ctgagactgg cgtctcacgc
     6421 tgggcccggg agcttctcag ggcggcgcga ccgccctaag gtcaccgtcg ccctgcgtgg
     6481 agcgcaggcg cgcgtcccgg gggatgggac ggaggcactc acacgaggtg ggaaagcatg
     6541 ctgagacgat cctggggact tctggccgtc gtgatggcgc tggccgcggt ggcgttcgcc
     6601 gcgccggcgg cggcgacgca ccgacccaac catggcggcg gaggcgatcc gagcccctcg
     6661 cccagcccca cggccaatgg cgaggacccg atcctgttcg tccacggctg gaactccagc
     6721 ggcacggtgt ggaccacgat gatcgaccgc ttcaaggctg acggctacac cgacggccag
     6781 ctcttcaact ggtcctacaa ctaccgccag tccaacgaga tcaccgcgca ggaggtcctg
     6841 gccaagatcc aggacatcaa gacccagacc ggggcggcga aggtggacgt catcacccac
     6901 tccatgggcg gcctgtcgtc ccgctggtgc gtcaagttca ccggctgcga cggcagcgtg
     6961 gatggctggg tgtcgctggg cgggcccaac cacggcacgg acacggcgaa cttctgcttc
     7021 gacacgtcgt gcaccgagat gcggatcggg tcggacttcc tcaccacgct gaacgcgggt
     7081 gacgagacac ccggcagcgc cacgcgctgg cgcacctggt ggtcgccgtg cgacagcacc
     7141 atcaacccgg acagctccgt gtcgctgtcc ggcgccacga acaccaagac cgcctgcctc
     7201 agccacagcg ccctgtacga ggacgcgacg gtgtacgggc aggtgcgcga cttcgtcggc
     7261 gcgtagcccc ggcacgaacg atgggccccg gccaggcggc cggggcccat ccgcgtccgc
     7321 ggggccggcc gagtccgccc ccggctggcg gtcagccggc cgaatccgcc cccggctggc
     7381 ggtcagccgg ccgagtccgc caggatgccg gcgagctcgc gggccagcgc cttcttggcc
     7441 tcgatgtcca tgccgcggcc gatctggatg cgctgcgcct ggggggagcc cgcgccgtgc
     7501 agcgactcgg tgaggtagcc caccgcgttg cggcccagcg tcaggttctc gatcagccgg
     7561 atgatgcgca tgcgcgcttc cgtgggcacg tcggcgcggc cggccaggag gcgctccacc
     7621 agggggccga cctcctcgct gcgcaggtcc gcctcgctgg gcatggtcgc gacc
//
LOCUS       DATMKC010000292         2910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_48746, whole genome shotgun sequence.
ACCESSION   DATMKC010000292 DATMKC010000000
VERSION     DATMKC010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48746"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..462
                     /locus_tag="VNU01_08785"
     CDS             208..462
                     /locus_tag="VNU01_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32754.1"
                     /translation="MTAQRGRPVVGAIAGLLLGVFLALDAVLLGLVPTNAAVLTVLPV
                     LGLALGVAVGLTAPFGRFRRARPPAEAEAAAEAEATEPAQ"
     gene            459..2744
                     /locus_tag="VNU01_08790"
     CDS             459..2744
                     /locus_tag="VNU01_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32755.1"
                     /translation="MRRLALLGVLTFTLTACGGLGTVNFGSTIGPGGGLVTSGDGKAT
                     AEIPPGALDQDTDVTLESDGDGGYVLGPEDAEFDEPVTVSIDGDEPGEPVRVDEDGTE
                     EPLDEPTILTGPGVFTSGGLVDEPGTIRRAPAADDVALSPRYLTVPVGAPFTAEASVA
                     RLAGDMDAAQWRESLDGVTAAGQAGAVATGQEVVSALKVPGASLGLGGAVGGGGLSTL
                     FGIVRLDFVCELPGRWAFGTDVPMADGRTMHWRTGTVDCLAQEDEDNFTEISGDPTVT
                     DPDDPPPPAGTGEVTTPAATGNPIDLLLKAPGTVAMADNSVAVRITPTEVEAEVGGEG
                     FEAKLDLWDIGRHTKEGQQALFTARAKADGEVAVAVGGGVSAPYPLKPGEPIFGLPLW
                     FDCLKEGTGTFGATISIDYTGTGGGTYEVTHVPGKVTCAPDPNPRRNLTIADPGVWYP
                     GGHVGVDGTSVRLHPTVFVAAPGETVTVNVEVGLPGDPARPYRRNASASAIVGEGDAV
                     TTEVNRIYGGYVPSPGTVQLPIRFTCVKEGVQAIGTRVFINSEHQTPEGKRTGATSPE
                     YDAQMLLGTGVCTKDVAGGVPAQDWMNRILGGGTAQEANLPGTLQGPCQGRAAIDGYG
                     TIDPRASGGVYKVPRKGSAKWFGAIAAVPGEERSHNGQVVVHLPPPFPAVKVGEWADT
                     TTEVHNNGEGSWDLNPQWTPGGVLVAVSGEHNDTAGNCRAKVLVRIDGGPLDSAAGPA
                     SLAFTLFALGIFGWAGLPKPS"
BASE COUNT          402 a         1073 c         1103 g          332 t
ORIGIN      
        1 gccagcgcgg cccgcacccc ctcggtcacg gccggcggga cctccacggc ggcgaacagc
       61 cgcagtctct ccacgctcat cgctccgacg ctacccgcgc ggggcacccc ggcgcgggcg
      121 accccgagcc cccctgcccg ccgcctcggt gcctatgatt gacccgtccg ccggcggggc
      181 gggcgagaag cggggaggag cggggccatg accgcacaga gagggcgtcc cgtcgtgggc
      241 gcgatcgccg ggctgctgct gggtgtgttc ctggcgctgg acgcggtgct gctggggctg
      301 gtgcccacca acgcggcggt gctgaccgtg ctgcccgtcc tggggttggc cctgggcgtg
      361 gccgtcggcc tgacggcgcc gttcgggcgc ttccggcgcg cgcggccccc ggccgaggca
      421 gaggcggcgg ccgaggccga ggcgacggag ccggcccagt gaggcgcctg gcgctgctcg
      481 gcgtcctgac gttcacgctg accgcctgcg gcggcctcgg cacggtgaac ttcggctcca
      541 ccatcggccc cggcggcggc ctggtcacca gcggtgacgg caaggccacg gcggagatcc
      601 cgccgggggc gctggaccag gacaccgacg tcacgctgga gagcgacggc gacggcggct
      661 acgtgctcgg accggaggac gccgagttcg acgagccggt gaccgtgtcc atcgacggcg
      721 acgagcccgg cgagcccgtg cgcgtcgacg aggacggcac cgaggagccg ctggacgagc
      781 ccacgatcct caccgggccg ggcgtgttca ccagcggcgg cctggtggac gagcccggca
      841 cgatccgccg ggcgcctgcc gcggacgacg tcgcgctgtc gccgcgctac ctcaccgtgc
      901 ccgtcggcgc gccgttcacc gcggaggcga gcgtcgcacg gctggccggc gacatggacg
      961 ccgcacagtg gcgcgagtcg ctggacggcg tgacggcggc cggtcaggcg ggcgccgtgg
     1021 cgaccggcca ggaagtcgtc tcggccctga aggtgccggg agcgtcgctg ggcctgggcg
     1081 gcgcggtggg cggcggtggc ctgtccacgc tgttcggcat cgtccggttg gacttcgtgt
     1141 gcgagctccc gggacgctgg gccttcggca ccgacgtccc gatggcggac ggccgcacga
     1201 tgcactggcg caccggcacg gtggactgcc tcgcgcagga ggacgaggac aacttcaccg
     1261 agatctccgg tgacccgacg gtgaccgacc cggacgatcc cccgccgccg gcggggacgg
     1321 gcgaggtcac cacaccggca gccaccggga acccgatcga cctgctgctg aaggcgcccg
     1381 gcaccgtggc catggccgac aacagcgtcg ccgtgcggat cacgcccacg gaggtggagg
     1441 ccgaggtcgg cggggagggc ttcgaggcca agctggacct gtgggacatc ggccgccaca
     1501 ccaaggaggg acagcaggcc ctgttcaccg cccgcgccaa agccgacggc gaggtcgcgg
     1561 tcgcggtcgg cggcggcgtc agcgcgccgt acccgctcaa gccaggcgag ccgatcttcg
     1621 gcctgccgct gtggttcgac tgcctcaagg aaggcaccgg cacgttcggc gccacgatct
     1681 cgatcgacta cacgggcacg ggcggcggca cctacgaggt gacgcacgtg ccgggcaagg
     1741 tcacctgcgc cccggacccg aacccccgcc gcaacctcac catcgccgat ccgggcgtct
     1801 ggtacccggg cggccacgtc ggcgtcgacg gcaccagcgt gcgcctgcac ccgaccgtgt
     1861 tcgtggcggc cccgggcgag acggtcacgg tcaacgtgga ggtcggcctg ccgggcgacc
     1921 ccgcccgtcc gtaccggcgc aacgcgtcgg cctccgcgat cgtcggcgag ggcgacgcgg
     1981 tgacgaccga ggtcaaccgg atctacggcg ggtacgtgcc gtcccccggc accgtgcagc
     2041 tgccgatcag gttcacctgc gtgaaggagg gagtgcaggc catcggcacc cgggtgttca
     2101 tcaacagcga gcaccagacc cccgagggca agcgcaccgg ggcgaccagc cccgagtacg
     2161 acgcccagat gctgctcggg acgggcgtgt gcaccaagga cgtcgccggc ggcgtgcccg
     2221 cgcaggactg gatgaaccgc atccttgggg gaggcacggc gcaagaggcc aacctgcccg
     2281 ggacgctgca gggcccctgc cagggccggg cggccatcga cggctacggc accatcgacc
     2341 cgcgcgccag cggcggcgtc tacaaggtgc cgcgcaaggg ctcggcgaag tggttcggcg
     2401 ccatcgcggc cgtgcccggc gaggagcgca gccacaacgg ccaggtcgtc gtgcacctgc
     2461 cgccgccgtt cccggcggtg aaggtgggcg agtgggccga caccaccacc gaggtgcaca
     2521 acaacggcga ggggtcctgg gacctcaacc cgcagtggac cccggggggc gtgctggtcg
     2581 cggtcagcgg cgagcacaac gacacggcgg gcaactgcag ggcgaaggtg ctcgtgcgca
     2641 tcgacggcgg gccgctggac tccgccgcgg gaccggcgag cctggcgttc acgctgttcg
     2701 cgctcggcat cttcggctgg gccggcctgc ccaagccgtc gtaggcgcta cccgagttcg
     2761 gtgagccgcc ggcgcagcca gtcgctggcc ccggcgacgc gcaccaggcg ggcgcccacg
     2821 ccgccgccgg tcaccgactc cgccagcccc agcgtcaggc cggcctcccg cagcaggcgc
     2881 gcgatcgcgt gctcgacgcc ctctccgtcc
//
LOCUS       DATMKC010000293         3748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_54860, whole genome shotgun sequence.
ACCESSION   DATMKC010000293 DATMKC010000000
VERSION     DATMKC010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54860"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VNU01_08795"
     CDS             complement(<1..904)
                     /locus_tag="VNU01_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWH32756.1"
                     /translation="MAERHEVDLLVIGAGPAGLYAAYYGGFRGLKVALMDSLPEAGGQ
                     VAALYPEKMIYDVAGFPKIKGQALIDNLVEQAAPFEPLYLLNHRAEKLEQAEDGVTIT
                     SHRGTVVACKAVIITGGIGTFTPRPLPNAEEFEGNGLVYFVPKLDVMAGKDVLIVGGG
                     DSAFDWALNLEPIAKSITLIHRRDRFRAHEHTVQQVMDSSVRVMTHTEVARIAGDGQI
                     EKVEVFDNQTEEREILQVHAVIAALGFTADLGPLKEWGLELDRRQIVVDTSMRTNIPR
                     VFAAGDITEYAGKVRLISVGFGEAA"
     gene            complement(988..3177)
                     /locus_tag="VNU01_08800"
     CDS             complement(988..3177)
                     /locus_tag="VNU01_08800"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32757.1"
                     /translation="MRRRFAASALLLTLLAIALAAAPGAPAGAQAVAVRTTTVFPGLH
                     RQVIDFRLDDGRTAVANVLSFSAADPNLELRPVLAQDRVPGLETVQSMGRRMAPRGGV
                     AGVNGGFWLNRPVGDPNSFLSIERALVSEAETQGAGPRGTLARRGDRGFVMDRLDTAI
                     RLQVATLSERAVTAVNRFPRATAPYPDDATGPVYLYTPHFGGQVTVEPLKVEEVPQPL
                     LVLVVEGLTPRASGVATGTVASSTTVAGPVAIPPGGTVIAAHGQAAARLAAAVPGDEV
                     TVEVTVKPLDSPATEWEDVVDALAAGPLIVRRGERVDPASWEDEGFAPTTHSNVRHPR
                     SAVALTADGRVLLVTVDGRQPGYSAGMTMHELSHFLRTLGVRDGLSLDGGGSSQMVVD
                     GVVKNRPCCDKPLRPVGTGLFVFHHSEFTATERIAGSGREATAAQVALTAYPDGAEEV
                     LLASAATYPDALAGGPLSHTLGAPLLLSGRDALPAATADALARLRPRRVTILGGGGAI
                     SAALEQQLRQDYEVRRLAGLGRVETALAIARAMGTEHERVFLARADRFPDALSAAAPG
                     GLLRMPILLTGPGELHAETAAHLERFGTREVVVLGGETAVSEAVTDELRARERKVTRL
                     AGTSRFGTAAAVNRWLMPQVPERDRTGLVVAAGGFFADALSGGPLAARRGQLLMIVPS
                     EDAMADPEAKAFLDELGEDVLQRVTLIGGHAVLSSYTQWQLDSIARR"
     gene            3309..>3748
                     /locus_tag="VNU01_08805"
     CDS             3309..>3748
                     /locus_tag="VNU01_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWH32758.1"
                     /translation="MHDRLSMLDASLLELESPTVPLHTGGLGVFAPGLTFADVHETLS
                     ARLDEIPLARQVVQPVPLGTGRPVWVDDNHFDLSYHLRHSALPPPGDADALAEVVSRL
                     FARPLDRSRPLWELYVIEGLEGGRTALFRKVHLVMVSGDAGDPFS"
BASE COUNT          476 a         1365 c         1404 g          503 t
ORIGIN      
        1 tggccgcctc gccgaagccg accgagatga gccggacctt gcccgcgtac tccgtgatgt
       61 ccccggcggc gaacacgcgg gggatgttgg tcctcatgct ggtgtcgacg acgatctggc
      121 gccggtccag ctccaggccc cactccttga gcgggccgag gtcggcggtg aagccgaggg
      181 cggcgatgac ggcgtgcacc tgcaggatct cgcgctcctc ggtctggttg tcgaacacct
      241 ccaccttctc gatctgcccg tccccggcga tgcgcgccac ctcggtgtgc gtcatgaccc
      301 ggaccgagga gtccatgacc tgctggacgg tgtgctcgtg ggcgcggaac cggtcgcggc
      361 ggtggatcag cgtgatggac ttggcgatcg gctccaggtt cagcgcccag tcgaacgcgc
      421 tgtcgccgcc gccgacgatg agcacgtcct tgcccgccat gacgtccagc ttgggcacga
      481 agtagacgag gccgttgccc tcgaactcct cggcgttggg cagcgggcgg ggcgtgaacg
      541 tgccgatgcc gccggtgatg atcacggcct tgcacgcgac cacggtgccg cggtggctgg
      601 tgatggtgac cccgtcctcg gcctgctcca gcttctccgc gcggtggttg agcaggtaca
      661 gcggctcgaa cggcgcggcc tgctccacca ggttgtcgat gagcgcctgc cccttgatct
      721 tggggaagcc ggccacgtcg tagatcatct tctcggggta cagcgccgcg acctgcccgc
      781 cggcttccgg cagcgagtcc atcagcgcca ccttcaggcc gcggaagccg ccgtagtagg
      841 cggcgtagag ccccgccggg ccggccccga tgaccagcag gtcgacttcg tgccgctccg
      901 ccaccgaacg tcctttcacc tgaggtaccg ccgcgcagtg gcgctcaagt taccccgtgg
      961 taacccggtt gagccaacca cgcgcgctca gcggcgggca atgctgtcga gttgccactg
     1021 ggtgtaggag ctgagcacgg cgtggccgcc gatcagcgtg acgcgctgga ggacgtcctc
     1081 gccgagctcg tccaggaacg ccttcgcctc ggggtcggcc atggcgtcct cgctcggcac
     1141 gatcatgagc agctggccgc gccgggccgc cagcgggccg ccggacagcg cgtcggcgaa
     1201 gaacccgccg gccgccacca ccaggccggt gcggtcgcgc tccgggacct gcggcatcag
     1261 ccagcggttg acggcggccg ccgtgccgaa gcggctcgtg ccggccaggc gggtcacctt
     1321 gcgctcgcgc gcgcgcagct cgtcggtcac cgcctcgctc acggcggtct cgccgcccag
     1381 caccacgacc tcgcgggtgc cgaagcgctc caggtgcgcg gcggtctcgg cgtgcagctc
     1441 gccgggaccg gtgagcagga tcggcatgcg cagcaggccg ccgggggccg ccgcggacag
     1501 cgcgtcgggg aaccggtcgg cgcgcgccag gaacacgcgc tcgtgctcgg tgcccatggc
     1561 gcgggcgatg gccagcgccg tctccacgcg cccgaggccg gccagccggc gcacctcgta
     1621 gtcctggcgc agttgctgct ccagggcggc gctgatcgcg ccgccgccgc ccaggatcgt
     1681 cacgcgccgg gggcgcagcc gcgccagcgc gtccgcggtc gcggccggca gcgcgtcacg
     1741 gccgctgagc agcagcggcg cgccgagggt gtgcgacagc ggacctcccg ccagggcgtc
     1801 cgggtacgtg gcggcggagg cgagcaggac ctcctccgcg ccgtcggggt aggcggtcag
     1861 ggcgacctgc gcggcggtcg cctccctccc ggagccagcg atgcgctcgg tggcggtgaa
     1921 ctcggagtgg tggaacacga acaggccggt gcccacgggc cgcagcggct tgtcgcagca
     1981 cggccggttc ttgaccaccc cgtccacgac catctggctg gagcccccgc cgtccagcga
     2041 caggccgtcg cgaacgccga gcgtgcgcag gaagtggctc agttcgtgca tcgtcatccc
     2101 cgcgctgtag ccgggttggc ggccgtccac ggtgaccagc agcacgcgcc cgtcggcggt
     2161 cagcgcgacg gctgagcgcg ggtggcgcac gttggagtgg gtggtggggg cgaagccctc
     2221 gtcctcccac gacgccgggt cgacccgctc gccgcggcgc acgatgagcg ggccggcggc
     2281 cagcgcgtcg accacgtcct cccactcggt ggccgggctg tccagcggct tcacggtgac
     2341 ctcgacggtc acctcgtcgc ccggcacggc ggcggccagg cgcgcggccg cctggccgtg
     2401 cgcggcgatc acggtgcccc ccggagggat cgcgaccggg ccggccacgg tggtcgacga
     2461 ggcgaccgtg ccggtggcga cgccggaggc ccgcggcgtg aggccctcca cgacgagcac
     2521 cagcagcggc tgcggcacct cctcgacctt cagcggctcc acggtgacct ggccgccgaa
     2581 gtgcggcgtg tacaggtaga cggggcccgt ggcgtcgtcg gggtacggag ccgtggcgcg
     2641 cgggaagcgg ttcacggccg tgacggcgcg ctcggacagg gtggcgacct gcagccggat
     2701 ggccgtgtcc aggcggtcca tgacgaagcc gcggtcgccg cgccgcgcca gggtgccgcg
     2761 cgggccggcg ccctgcgtct cggcctccga caccagcgcg cgctcgatcg acaggaagct
     2821 gttcgggtcg ccgaccggcc ggttgagcca gaagcccccg ttcacgcccg ccacgccgcc
     2881 gcgcggcgcc atgcgccggc ccatcgactg caccgtctcc agcccgggga cgcggtcctg
     2941 cgccagcacg ggccgcagtt ccaggttggg gtcggccgcg gagaacgaca gcacgttggc
     3001 gacggcggtg cgcccgtcgt ccaggcggaa gtcgatcacc tgccggtgca ggccggggaa
     3061 caccgtggtg gtgcgcacgg ccacggcctg ggcgcccgcg ggcgcgccgg gcgcggcggc
     3121 cagggcgatg gccagcaggg tcagcaggag ggcggaagca gcgaaacggc gacgcacagt
     3181 ggtctctcgg gctcgtgcag ggcttggcgg gtcgggccgc cggttaggct agccgcgcac
     3241 gggtcgccgg accggcgatt cggcggaatt cacgccgccg gccccaagcc ggcaaggagt
     3301 gcctgcgaat gcacgaccgc ctgtccatgc tcgacgcctc gctgctcgag ctggagtctc
     3361 ccacggtgcc gctgcacacc ggcggcctcg gcgtgttcgc gcccgggctc accttcgccg
     3421 acgtgcacga gacgctgagc gcgcggctgg acgagatccc gctggcgcgc caggtggtgc
     3481 agccggtgcc gctgggcacc ggacggcccg tgtgggtgga cgacaaccac ttcgacctgt
     3541 cctaccacct gcgccattcc gcgctgccgc cgccgggcga cgccgacgcg ctcgcggaag
     3601 tggtgtcgcg cctgttcgcc cgcccgctgg accgcagccg cccgctgtgg gagctgtacg
     3661 tgatcgaggg gctggagggc ggccgcaccg cgctgttccg caaggtgcac ctggtcatgg
     3721 tgagcggcga cgccggcgac cccttctc
//
LOCUS       DATMKC010000294         2990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_1893, whole genome shotgun sequence.
ACCESSION   DATMKC010000294 DATMKC010000000
VERSION     DATMKC010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1809
                     /locus_tag="VNU01_08810"
     CDS             124..1809
                     /locus_tag="VNU01_08810"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWH32759.1"
                     /translation="MRRVAAEAEQAAQQLARQTITPEALARSRHELVAGELALRNLSQ
                     GVRDNGVAPVLRDALRPTTRVDDEATARARAEARDAVAPVVRTYPRGSVIVQAGQPVD
                     EAQLEALRSRGLEGAEPWLVVARSVLVALGLTVVVGAVLRTRFPEVWRSGRRLLLLAS
                     IVALFSATAEALWLFMPEEWPALLFLVPSGAVAMLAAVLFSPGVASLLAAPITVLVAY
                     TMPGHIDVAAYTAAVYLGSVPLGVRLGAWNALTRVAWQSSLIYGAAAGVAALAFGRPE
                     QVPVAIVAGLAGGVLTAVIVNGSLPFMESLFGMVTPTSLMRLSNRNHPLLRELEQKAL
                     GSYNHSIMVATLCERACRAIDADALLASTAALYHDIGKVRRPYFFVENQFGVGNPHDD
                     LEPRVSAIIIQEHVSDGIAMARTFRLPPEIVEGIATHHGTTLVSYFHAKALQRYGAER
                     VTEEEFRYPGRKPASREMAVLMLADCCEGATRAVAIDNRNLTRADLEEIVHRLIDERV
                     ADGQLDESALTLRDLKLVRESLIDTLVGVYHPRIAYPDPQVPEHTTEDAPDEA"
     gene            complement(1823..2905)
                     /locus_tag="VNU01_08815"
     CDS             complement(1823..2905)
                     /locus_tag="VNU01_08815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWH32760.1"
                     /translation="MPSLTARGARTAALAALGLLLMLALLGGLLVRGQVQAREDLRTR
                     FALRAELAAGFMDSLRTQGHDLRLAHEAAEASAHLATIVPVAGQRAYLVDRGLRVLAA
                     SPVDAVGRLLPEVEPALGAPTPGATPTYRGIHHPGEHVAASAAVGASGLMVVITVPTQ
                     RLYQPLEGAVWPQWLLFTVFAVVGAMAVLLLLRLMRREAELEWAMRVDPVTGVRNRRH
                     LQERLPEVASASRRHGRPWALLMIDVDHFKRVNDEQGHAGGDAVLREVAAAIVAECRV
                     EDLAGRWGGDEFLAILPDSDLDAAEHLAARIAERARRRMGAQGSLSVGCAAGLGEDPE
                     GMLRRADAALYEAKRQGRGRVVAAAE"
BASE COUNT          366 a         1142 c         1110 g          372 t
ORIGIN      
        1 tcaggcccag cgcgaccagg acggagcgcg ccacgaccag ccacggctcg gcaccctcca
       61 gcccccggct gcgcagcgcc tccagctgcg cctcgtccac cggctggccc gccggacgac
      121 acgctgcggc gcgtggcggc ggaggcggag caggcggcgc agcagctcgc gcggcagacc
      181 atcacgccgg aggcgctcgc gcgctcccgc cacgagctcg tcgccgggga gctggcgctg
      241 cgcaacctgt cccagggcgt ccgcgacaac ggggtggcgc ccgtcctgcg cgacgcgctg
      301 cgccccacca cgcgggtgga cgacgaggcc acggcgcgcg cgcgggcgga ggcgcgcgac
      361 gcggtggcgc ccgtggtgcg cacctatccc cgtggcagcg tgatcgtcca ggcgggccag
      421 ccggtggacg aggcgcagct ggaggcgctg cgcagccggg ggctggaggg tgccgagccg
      481 tggctggtcg tggcgcgctc cgtcctggtc gcgctgggcc tgacggtggt cgtcggcgcg
      541 gtgctgcgca cgcgcttccc cgaggtgtgg cgctccggcc gccgcctgct gctgctggcc
      601 tccatcgtcg cgctgttcag cgccacggcc gaggcgctgt ggctgttcat gccggaggag
      661 tggccggcgc ttttgttcct ggtgccatcc ggggccgtgg cgatgctggc ggccgtgctg
      721 ttctcccccg gcgtggcgtc cctgctggcc gccccgatca ccgtgctggt ggcctacacg
      781 atgccgggcc acatcgacgt ggcggcctac accgcggcgg tctacctggg cagcgtgccg
      841 ctgggcgtgc gcctgggcgc gtggaacgcg ctgacgcgcg tggcgtggca gtcctcgctg
      901 atctacgggg ccgcggcggg cgtcgcggcg ctcgcgttcg ggcggcccga gcaggtcccg
      961 gtggcgatcg tcgccggcct ggccggcggc gtgctcaccg ccgtgatcgt caacggcagc
     1021 ctgccgttca tggagtcgct gttcgggatg gtgacgccca ccagcctgat gcggctgtcc
     1081 aaccgcaacc acccgctcct gcgcgagctg gagcagaagg cgctgggctc ctacaaccac
     1141 tcgatcatgg tggccacgct gtgcgagcgc gcgtgccgcg ccatcgacgc cgacgccctg
     1201 ctggcgtcca cggcggccct gtaccacgac atcggcaagg tgcgccggcc ctacttcttc
     1261 gtggagaacc agttcggcgt cggcaacccg cacgacgacc tggagccgcg cgtctcggcg
     1321 atcatcatcc aggagcacgt cagcgacggc atcgccatgg cgcgcacgtt ccggctgccg
     1381 ccggagatcg tggagggcat cgccacccac cacggcacca cgctggtgag ctacttccac
     1441 gccaaggcgc tgcagcgcta cggcgccgag cgcgtcaccg aggaggagtt ccgctacccc
     1501 ggccgcaagc cggcctcccg ggagatggcg gtgctcatgc tggcggactg ctgcgagggc
     1561 gcgacccgcg ccgtcgcgat cgacaaccgc aacctcaccc gcgcggacct ggaggagatc
     1621 gtccaccggc tgatcgacga gcgcgtcgcc gacggccagc tcgacgagtc ggcgctcacg
     1681 ctgcgcgacc tgaagctggt gcgcgagtcg ctgatcgaca cgctggtggg cgtctaccac
     1741 ccgcgcatcg cctacccgga tccccaggtg ccggagcaca cgaccgagga cgcgcccgac
     1801 gaggcctgag gcgccggccc gctcactccg cggccgccac cacccggcct cgcccctgcc
     1861 gcttcgcctc gtacagcgcc gcgtcggcgc ggcgcagcat cccttcgggg tcctcgccca
     1921 gccccgcggc gcaaccgacg ctgagggagc cctgcgcgcc catccgccgg cgcgcgcgct
     1981 cggcgatgcg ggccgccagg tgctcggcgg cgtcgaggtc gctgtccggc aggatggcga
     2041 ggaactcgtc gccgccccag cggccggcca ggtcctcgac ccggcactcg gccacgatgg
     2101 cggccgcgac ctcgcgcagg acggcgtcgc cgccggcgtg gccctgctcg tcgttgaccc
     2161 gcttgaagtg gtcgacgtcg atcatgagca gcgcccacgg gcgcccgtgg cggcgggacg
     2221 cgctggcgac ctcgggcagc cgctcctgca ggtggcggcg gttgcgcacg ccggtgaccg
     2281 ggtcgacgcg catcgcccac tccagctcgg cctcccggcg catcaggcgc agcagcagca
     2341 gcacggccat ggcgcccacc acggcgaaca cggtgaacag cagccactgc ggccagaccg
     2401 cgccctccag cggctggtac aggcgctgcg tcggcacggt gatcaccacc atcaggccgg
     2461 acgcgcccac cgccgcgctg gccgcgacat gctcgccggg gtggtggatc ccgcggtagg
     2521 tcggcgtggc cccgggcgtg ggcgcgccga gcgcgggctc cacctcgggg agcagccggc
     2581 cgacggcgtc gacggggctg gccgcgagca cgcgcaggcc gcggtcgacc aggtacgcgc
     2641 gctgaccggc gaccggcacg atggtggcca ggtgcgcgga ggcctcggcc gcctcgtgcg
     2701 ccaggcgcag gtcgtggccc tgggtgcgca gcgagtccat gaacccggcg gccagctcgg
     2761 cgcgcagcgc gaaccgcgtg cgcaggtcct cccgcgcctg cacctgcccg cgcaccagca
     2821 ggccgcccag cagcgcgagc atgagcaaga gccccagcgc cgccagggcg gcggtgcggg
     2881 ctccccgcgc tgtcaggctc ggcactggcg ccattgcggc acggtatccc gggcgggcgt
     2941 gatcgacgcg gacttgcggc acccggtttc agtcgccggc gagctccggc
//
LOCUS       DATMKC010000295         3028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_194472, whole genome shotgun sequence.
ACCESSION   DATMKC010000295 DATMKC010000000
VERSION     DATMKC010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194472"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VNU01_08820"
     CDS             <1..742
                     /locus_tag="VNU01_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH32761.1"
                     /translation="FLDHSVSTAKLLPESVNAAVDADVIVIDATASLGNASMTCRAAA
                     ASDLQRPILLVAGEGGLAALKAGWGFDDWVSPAAQPAEVETRLRLLLERSGGSGSARR
                     AAPTTIGDLAIDEDTYVVRLRGVPLDLTYREFELLKYLAANPGRVFTRGQLLLEVWGY
                     DYYGGTRTVDVHIRRLRAKLGPEYEHLIGTVRGVGYKLDPQGLSGALTDDEDEASAGE
                     DDGSGTGGAAATDVDLRDASAGGSGRRR"
     gene            complement(744..1721)
                     /gene="mshD"
                     /locus_tag="VNU01_08825"
     CDS             complement(744..1721)
                     /gene="mshD"
                     /locus_tag="VNU01_08825"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HWH32762.1"
                     /translation="MPPVRYEIATELRQPVLAEILALIEHCTRVEGHRPVGEHKYAHL
                     LGAPGGWTGVLARRADELVGYAHLRWNPAGDGPRLAAEVVVHPGEDRDEVARHLVEEV
                     RALLARAGGGVLWLWVHRVQDAATTLAARMGFSIQRELAFMTRRLAAAPEWRVPDGVT
                     VRPYRPGPDDAALLRVNNAAFAGHPENGGWTAATLEQRRSLDWFDPAGVLLAWRGERL
                     LGFHWTKWHGHDSDEVPVHEPVGEVYVLAVDPVAHGTGLGRLLLRAGLAHLHGRGCRV
                     AVLYVDAASGPALALYRSEGFVEEYREVCYEAVVPPEPAQPAVDLLRPA"
     gene            1764..2189
                     /locus_tag="VNU01_08830"
     CDS             1764..2189
                     /locus_tag="VNU01_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWH32763.1"
                     /translation="MDVAQRLREQGIRMTPQRRVVCDTLARADRHLTAEEVHARASGD
                     TPELNLASVYRTLALLGELGLARQVHFGDGRSHWELAHPDDAFHLVCRGCGEVTHHAG
                     DLVDRVRDHLARGHGFTPEGVDLVVRGLCRDCAKRPSPS"
     gene            2256..2327
                     /locus_tag="VNU01_08835"
     tRNA            2256..2327
                     /locus_tag="VNU01_08835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2289..2291,aa:Lys,seq:ttt)
     gene            2356..2433
                     /locus_tag="VNU01_08840"
     tRNA            2356..2433
                     /locus_tag="VNU01_08840"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2391..2393,aa:Asp,seq:gtc)
     gene            2488..2563
                     /locus_tag="VNU01_08845"
     tRNA            2488..2563
                     /locus_tag="VNU01_08845"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2521..2523,aa:Phe,seq:gaa)
     gene            2717..2902
                     /locus_tag="VNU01_08850"
     CDS             2717..2902
                     /locus_tag="VNU01_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32764.1"
                     /translation="MRRMLTMVAAAAVTALAAVPVLADAPVDGQLCGDVVVVVNGEAV
                     IDEFHCLPPEGEPGLPI"
BASE COUNT          423 a         1114 c         1103 g          388 t
ORIGIN      
        1 gttcctggac cactccgtgt cgacggccaa gctgctgccg gagtcggtca acgcggccgt
       61 ggacgccgac gtgatcgtga tcgacgccac cgccagcctg ggcaacgcgt cgatgacctg
      121 ccgcgcggcg gcggcctcgg acctgcagcg gccgatcctg ctggtggccg gcgaaggcgg
      181 cctcgcggcg ctcaaggcgg gctggggctt cgacgactgg gtctccccgg cggcgcagcc
      241 cgcggaggtg gagacgcggc tgcggctgct gctggagcgc agcggcggga gcggttccgc
      301 ccggcgcgcg gcccccacca cgatcggcga cctggccatc gacgaggaca cctacgtggt
      361 gcgcctgcgc ggcgtgcccc tggacctcac ctaccgcgag ttcgagttgc tcaagtacct
      421 ggcggccaat cccgggcgcg tgttcacgcg ggggcagctg ctcctggagg tctggggcta
      481 cgactactac ggcggcacgc gcaccgtcga tgtccacatc cggcgactgc gcgccaagct
      541 ggggccggag tacgagcacc tcatcggcac ggtccgcggc gtcggctaca agctggaccc
      601 gcaggggctg tccggcgccc tcaccgacga cgaggacgaa gccagcgcgg gcgaggacga
      661 cgggtccggc accggtggcg ccgcggcgac cgacgtcgat ttgcgcgacg cctcggccgg
      721 cggctccggc cggcgccgct gagctacgcc ggcctgagca ggtccacggc cggctgggcc
      781 ggctccggcg ggaccacggc ctcgtagcac acctcgcggt actcctcgac gaagccttcg
      841 gagcggtaca gcgccagcgc cggaccactc gccgcgtcga cgtagagcac cgcgacccgg
      901 cagccgcgcc cgtgcaggtg cgccaggccg gcacgcagga gcaggcggcc caggcccgtg
      961 ccgtgggcca ccgggtcgac cgccagcacg tagacctcgc cgaccggctc gtggaccggc
     1021 acctcgtcgg agtcgtggcc gtgccacttg gtccagtgga agccgagcag gcgctcgccg
     1081 cgccatgcca gcagcacgcc ggccgggtcg aaccagtcca ggctgcgccg ctgctccagc
     1141 gtcgccgccg tccagccgcc gttctccggg tggcctgcga acgcggcgtt gttgacgcgc
     1201 agcagcgcgg cgtcgtccgg cccgggtcgg tacggccgca cggtcacccc gtcgggcacc
     1261 cgccactccg gcgcggcggc caggcggcgc gtcatgaacg cgagctcacg ctggatcgag
     1321 aagcccatgc gcgcggccag cgtcgtggcc gcgtcctgga cgcggtgcac ccacagccac
     1381 aggactcccc ctcccgcccg tgcgagcagc gcgcgcacct cctccaccag gtgccgggcg
     1441 acctcgtcgc ggtcctcgcc ggggtggacc accacctccg ccgccagcct cggaccgtcg
     1501 ccggcaggat tccagcgcag gtgcgcgtag cccacgagct cgtccgcgcg ccgggccagc
     1561 acaccggtcc acccgcccgg cgctcccagg aggtgggcgt acttgtgctc cccgaccggg
     1621 cggtggccct ccacacgggt gcagtgctcg atcagcgcca ggatctcggc caggaccggc
     1681 tggcgcagct cggtggcgat ctcgtagcgc acgggaggca tgccgccgag cctagccgcg
     1741 gcgcgcgctg gcagaatcat ggcgtggacg tggcccagcg cctgcgcgag cagggcatcc
     1801 ggatgacccc gcagcggcgg gtggtctgcg acacgctcgc gcgcgccgac cggcacctga
     1861 ccgccgagga ggtgcacgcc cgcgccagcg gcgacacgcc ggagctcaac ctcgccagcg
     1921 tgtaccggac actggccctg ctgggcgagc tgggcctggc gcggcaggtg cacttcggcg
     1981 acgggaggag ccattgggaa ctggcccatc ccgacgacgc cttccacctg gtgtgccggg
     2041 gctgcggcga ggtgacccac cacgcgggcg acctggtgga ccgcgtgcgg gaccacctgg
     2101 cgcgcgggca tggcttcacg ccggagggcg tcgacctggt ggtgcgcggc ctgtgccgcg
     2161 actgcgccaa gcggccctcg cccagctagc acgcacgcga gcgggcggct acactcgcgc
     2221 agcaccgcgc cgggaggcgg agtaccctgc tcgacgggcc gttagctcaa cgggtagagc
     2281 tccggacttt taatccggcg gagcgggttc gagccctgca cggcccacgg acgacgcgag
     2341 acccggtgtg acaatggagc tgtggagcag ttaggagtgc tcgtcaccct gtcaaggtga
     2401 aggtcgcggg ttcaaatccc gtcagctccg ccagccggcc ggcaatcccc ccagtgatgg
     2461 gcagggcgcc ggccggtcgc atgaaacggt caggtagctc agttggcaga gcgcgcgact
     2521 gaaaatcgtg aggtcggcgg ttcaaccccg cccctgacca ccacaggggc ccgtcaccgg
     2581 cgggcccctt gtcgttgtcc cgtcgcggtg acgcggagga aaaatctccg ccgcggaccg
     2641 tgaggcacgg cggacacacg cgttccccgg tcgaacgtgg cgcgcacgcg cccgagcgga
     2701 caaggagcgc acgacgatgc gacgcatgct gacgatggtg gcggccgcgg ccgtcacggc
     2761 cctggcggcg gttccggtcc tggcggacgc cccggtggac ggccagctct gcggtgacgt
     2821 ggtggtggtc gtgaacggcg aggccgtgat cgacgagttc cactgcctgc cgccggaggg
     2881 cgagccgggc ctgccgatct gacagcgtcc cgccccacca acagggacgg cgaaggggac
     2941 accacccatc cggtgtcccc ttcgtgctgt tttttccttt actgggagga gcggattccg
     3001 taccatttcc cccggacggc cgccttcc
//
LOCUS       DATMKC010000296         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_166459, whole genome shotgun sequence.
ACCESSION   DATMKC010000296 DATMKC010000000
VERSION     DATMKC010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166459"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VNU01_08855"
     CDS             <1..688
                     /locus_tag="VNU01_08855"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWH32765.1"
                     /translation="DTRKRASQVIREVLDFPYSHVVVVSGPNLAKEVARRMPGGTVAA
                     GPETQVVRRVQQAGHTPYFRVYTNPDVVGVEIGGAVKNAIALAAGMADGMGFGDNTKA
                     MLITRGLAEMTRLGVALGGNPLTFSGLAGMGDLVATCMSRQSRNRHVGEELAKGHTLE
                     EVIAAMNMVAEGVKSSKALLEIATEQGVEMPIVEHVVKVVHEGLSPREMLTSLMTRSP
                     KAEFHGMPDL"
     gene            685..1818
                     /locus_tag="VNU01_08860"
     CDS             685..1818
                     /locus_tag="VNU01_08860"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HWH32766.1"
                     /translation="MSAGEAAGRTRVLVLFGGRSSEHEVSCLSARNVLGAIDRERYVV
                     TTVGITRDGRWTLLDGVPDTPPGVLPAVPDDGPTVALVRTRKGPVLLRYDEGGGAAED
                     LGPVDVCFPVLHGPYGEDGTVQGLLASHGVPYVGADVASSAVGVDKRQMKNVFKARGL
                     PQVAHLAVRRTVWDTDRGPVLDDVETTLRYPVFTKPARQGSSIGIRKCASREELIAGI
                     EEAFGYDRVAIVEEGLDGVRELECGVIGNSQIEVTRPGETVHVGEAFYDFDAKYLKPV
                     ELRCPADLPEHVQRRCMDFAREAYLAIGCRGMARVDFFYDERNPDPAGQLLINEINTI
                     PGLTPSSMFPPVWAAEGVGFGELIDRLLSLALSASRAEAHYAP"
     gene            1870..2523
                     /locus_tag="VNU01_08865"
     CDS             1870..2523
                     /locus_tag="VNU01_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWH32767.1"
                     /translation="MTRYADRRDAGRRLAEALRDHLRGETRRVVVLAIPRGGVPVAAE
                     VAEALDAALDVVVVRKLRAPFQPELGYGAIGSDGHVDVDRDMAARLGLTEEQVQAEVA
                     DRRAVVDQRVREYRRVLPQPDLGGAVAVVVDDGIATGSTARQACGLARRLGAARVVLA
                     APVGPPDVEAALAGVADDVVVPARPPDFQAVGQAYTDFAQLDDGDVLDALRSAAGRV"
BASE COUNT          354 a          921 c          998 g          336 t
ORIGIN      
        1 ggacacgcgc aagcgcgcga gccaggtcat ccgcgaggtg ctggacttcc cgtactccca
       61 tgtcgtggtg gtcagcggtc cgaacctcgc caaggaggtg gcccggcgca tgcccggcgg
      121 caccgtggcg gccggtccgg agacgcaggt ggtgcggcgg gtgcagcagg ccggccacac
      181 gccctacttc cgcgtctaca ccaacccgga cgtggtgggc gtggagatcg gcggcgcggt
      241 caagaacgcg atcgcgctgg ccgccggcat ggccgacggc atgggtttcg gcgacaacac
      301 caaggcgatg ctgatcacgc gcggcctggc ggagatgacg cgcctgggcg tggcgctggg
      361 cggcaacccg ctgacgttct ccgggctggc gggcatgggc gacctggtgg ccacgtgcat
      421 gagccgccag tcgcgcaacc gtcacgtcgg cgaggaactg gccaagggcc acacgctgga
      481 ggaggtgatc gccgcgatga acatggtcgc cgagggcgtg aagagctcca aggcgctgct
      541 ggagatcgcg accgagcagg gcgtggagat gcccatcgtc gagcacgtgg tcaaggtggt
      601 ccacgagggc ctgtcgccgc gcgagatgct gacgtcgctg atgacgcgca gtcccaaggc
      661 cgagttccac gggatgccgg acctgtgagc gccggggagg ccgcgggccg cacgcgcgtc
      721 ctggtcctgt tcggcgggcg ctccagcgag cacgaggtga gctgcctgtc ggcgcgcaac
      781 gtgctgggcg ccatcgaccg cgagcgctac gtggtgacca cggtgggcat cacgcgcgac
      841 ggccgctgga ccctgctgga cggcgtgccg gacacgccgc cgggcgtgct gccggccgtg
      901 ccggacgacg gccccaccgt ggcgctggtg cgcacgcgca agggaccggt gctgctgcgc
      961 tacgacgagg gcggcggcgc cgccgaggac ctggggccgg tggacgtgtg cttccccgtg
     1021 ctgcacgggc cctacggcga ggacggcacg gtgcagggac tgctcgccag ccatggcgtg
     1081 ccgtacgtgg gcgcggacgt ggcctccagc gccgtcggcg tggacaagcg ccagatgaag
     1141 aacgtcttca aggcgcgcgg cctgccgcag gtcgcgcacc tggcggtgcg gcgcacggtg
     1201 tgggacaccg accgcgggcc ggtgctggac gacgtcgaga cgacgctgcg ctacccggtg
     1261 ttcaccaagc ccgcgcgcca gggcagctcc atcggcatcc gcaagtgcgc cagccgcgag
     1321 gagctcatcg ccggcatcga ggaggccttc ggctacgacc gcgtcgcgat cgtggaggag
     1381 gggctggacg gcgtgcgcga gctggagtgc ggcgtcatcg gcaactccca gatcgaggtg
     1441 acccggccgg gcgagacggt gcacgtgggc gaggcgttct acgacttcga cgccaagtac
     1501 ctgaagcccg tggagttgcg ctgccccgcg gacctgccgg agcacgtgca gcgccgctgc
     1561 atggacttcg cgcgcgaggc gtacctggcg atcggctgcc gcggcatggc gcgcgtggac
     1621 ttcttctacg acgagcgcaa cccggacccg gccggccagc tgctgatcaa cgagatcaac
     1681 accatcccgg gcctcacgcc gtcgtcgatg ttcccgcccg tgtgggcggc cgagggcgtg
     1741 ggcttcggcg agctgatcga ccgcctgctg tccctggcgc tgtcggcgtc gcgcgcggag
     1801 gcgcactacg ccccctgacg ccgcaggccc cgcacccata agacgccgga agctggggat
     1861 accacggaca tgacccgcta cgccgaccgt cgggacgccg gccgccgcct cgccgaggcg
     1921 ctgcgcgatc atctgcgcgg cgagacgcgg cgtgtcgtcg tgctcgcgat cccgcggggc
     1981 ggcgtgccgg tcgccgcgga ggtcgccgag gcgctggacg cggcgctgga cgtcgtggtg
     2041 gtgcgcaagt tgcgcgcccc gttccagccc gagctcggct acggggcgat cggctccgat
     2101 ggccacgtcg acgtcgaccg cgacatggcc gcgcgcctcg gcctcacgga ggagcaggtc
     2161 caagccgagg tcgccgaccg ccgtgccgtc gtggaccagc gtgtccgcga gtaccgccgc
     2221 gtcctgcccc agccggacct gggcggcgcc gtggcggtcg tcgtcgacga cggcatcgcc
     2281 acgggcagca ccgcccgcca ggcgtgcggg ctggcgcgcc gcctgggcgc ggcccgcgtc
     2341 gtcctggcgg cgccggtcgg gccgccggac gtcgaggccg ccctcgcggg cgtcgccgac
     2401 gacgtcgtgg tgccggcccg cccgccggac ttccaggcgg tcggccaggc ctacaccgac
     2461 ttcgcgcagc tggacgacgg cgacgtgctc gacgccctgc gcagcgcggc cgggcgggtc
     2521 tagcgctcca cctcgaacac caggcgcacc tgcgtgcgga actccgtcac ctcgccgccc
     2581 tcgaccacgg cgctggtgcc caggatgtc
//
LOCUS       DATMKC010000297         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_313387, whole genome shotgun sequence.
ACCESSION   DATMKC010000297 DATMKC010000000
VERSION     DATMKC010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313387"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..215
                     /locus_tag="VNU01_08870"
                     /pseudo
     CDS             78..215
                     /locus_tag="VNU01_08870"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            255..>2210
                     /locus_tag="VNU01_08875"
     CDS             255..>2210
                     /locus_tag="VNU01_08875"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32768.1"
                     /translation="MRRHRGATRIETAVEVAKAGAPSATEVIVARAFGGEGDPTQAFA
                     DSLAAGAWAASSGVPVLLTESGTLSAATQAYLESSKPARAFVVGGTGAVSDAVLAQIA
                     SMGIQTERVSGPTRFDTAVEIAKQRGMDDATDGRGVALIEGQAEGAWAPGFAAAGHAA
                     RKRLAVLLANGDALPEPTAAYLAGGDDATELVCAAQLAEAACDAASQALGKQLAPTVT
                     LNEGSVAQYADVTGTVEPPEEVLSLTVSGCGLADRSVLVDADGSYATRIEGSPGECTL
                     TFTLKTRTRGTFTKTFSLTVTEAAPATSNPELVEAQVVSTGAQVATVRYVFSEVVRAT
                     SYENTATDRIPGTQFVLYAPRGVQTTSPQSDTRFQAQDPLTNPTTDLGSSPRRDPDNN
                     RAVLVDFLVAHVQKATTAVALKEAAHSVDVVEGQSYQPSLPGSAPLKQVQFPEGRTDL
                     SDLVSVGSVTAQNMPPGEFKATFTFDLKEGQTEADSPTNASAYGVVLADGRIIPGKGT
                     PARSGTTATVTFAASDTRTPDGTVCTEGNCPPLQEALTGVRRGYVLGDGTRPLQAAPV
                     VSDGVTVGPDLVSVAIDVAGGTADFTFDDTVSSPDVSKFSLYLVNGSQLTPTEPSIVS
                     QRVVRVKAAVSKLVVGGGVAAQAVSGPG"
BASE COUNT          328 a          830 c          786 g          266 t
ORIGIN      
        1 ctcggcggcg gcactcggcg ccagcgacag gaccaggccg agcgtgagca cgcccgccag
       61 caccgttcgc cgactcgctc gcctcgggcg tgctccaggc caccgcgccc ctgctgctga
      121 cccccaccgc cagcctggcc cccgccgtgg actccgagct caagcgcctc gacgtggacg
      181 tcgtccacat cctgggcggc cccagcgccg tgtccaccgc cgtggaggac tcgctcaccc
      241 agcagggcta caccgtgcgc cgccaccgcg gcgccacccg catcgagacg gccgtggagg
      301 tcgccaaggc cggcgcgccg tcggcgacgg aggtcatcgt cgcgcgcgcc ttcggtgggg
      361 aaggcgaccc gacgcaggcg ttcgccgact cgctggccgc aggcgcgtgg gccgcgtcca
      421 gcggcgtccc cgtgctgctg acggagagcg gcacgctgtc cgccgccacg caggcctacc
      481 tggagagctc gaagccggcc cgcgccttcg tcgtcggtgg cacgggcgcc gtcagcgacg
      541 ccgtgctcgc gcagatcgcc agcatgggca tccagaccga gcgcgtgtcc ggcccgacgc
      601 gcttcgacac cgccgtggag atcgccaagc agcgcggcat ggacgacgcg accgacggcc
      661 gcggcgtcgc gctgatcgaa ggccaggccg aaggcgcgtg ggcgcccggc ttcgccgccg
      721 cgggccacgc cgctcgcaag cgcctggccg tgctgctcgc caacggcgac gcgctgccgg
      781 agccgaccgc cgcgtacctg gccggcggcg acgacgccac cgagctggtg tgcgcggcgc
      841 agctcgccga ggcggcgtgc gacgcggcct cgcaggccct gggcaagcag cttgccccca
      901 cggtgaccct gaacgagggc tccgtggcgc agtacgccga cgtgaccggc acggtggagc
      961 caccggagga ggtgctgagc ctcacggtgt ccggctgcgg cctggctgac cggtctgtgc
     1021 tggtggacgc cgacggcagt tacgcgacgc gcatcgaggg ctcgccgggg gagtgcacgc
     1081 tgacgttcac attgaagacc aggacgcgcg gcacgttcac caagacgttc tcgctgacgg
     1141 tcacggaggc ggcgccggcg acgtccaatc cggagctggt cgaggcgcag gtcgtctcga
     1201 cgggcgcgca ggtcgccacc gtgcgctacg tgttcagcga ggtggtccgc gcgacgagct
     1261 atgagaacac agcgaccgac cggatccccg gcacgcagtt cgtcctctac gcgccccgcg
     1321 gcgtgcagac cacaagcccc cagagcgaca cccgcttcca ggcacaagac ccgctgacga
     1381 accccaccac cgatctgggg tcgtcgccgc gccgtgaccc ggacaacaat cgtgcggtac
     1441 tggtggactt cctggtcgcg cacgtccaga aggccaccac cgccgtggcc ctgaaggaag
     1501 cggcccactc cgtggacgtc gtcgagggac agagctatca gcccagcctg ccgggcagcg
     1561 cgccgctgaa gcaggtgcag ttcccggagg gccgcaccga cctgagcgat ctcgtgtccg
     1621 tcggctccgt gacggcgcag aacatgccgc ccggcgagtt caaggcgacc ttcacattcg
     1681 acctgaagga gggccagacc gaggcggact ccccgacgaa cgcctcggcc tacggcgtcg
     1741 tgctcgccga cgggcgcatc atccccggca agggcacccc ggcccgcagc ggcacgaccg
     1801 cgacggtcac gttcgcggcc agcgacacgc gcacaccgga cggcaccgtc tgcacggaag
     1861 gcaactgccc gccgctgcag gaggccctga ccggcgtgcg gcgcggctac gtgctgggcg
     1921 acggcacgcg cccgctccag gcggcgcccg tggtgagcga cggtgtgacc gtcggcccgg
     1981 acctggtgag cgtcgccatc gacgtcgccg gcggcacggc ggacttcacc ttcgacgaca
     2041 ccgtgtccag ccccgacgtg tccaagttca gcctgtatct ggtgaacggg tcgcagctca
     2101 cgccgacgga accgtccatc gtgagccagc gcgtcgtccg cgtgaaggcg gcggtcagca
     2161 agctggtggt cggcggcggc gtggccgctc aggcggtcag cgggcccggt
//
LOCUS       DATMKC010000298         3727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_335834, whole genome shotgun sequence.
ACCESSION   DATMKC010000298 DATMKC010000000
VERSION     DATMKC010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335834"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VNU01_08880"
     CDS             complement(<1..789)
                     /locus_tag="VNU01_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32769.1"
                     /translation="MTDAVVFDAVRTPRGRGKATGALHAVKPISLVTQLIAALGERNP
                     SLDRGTIDDIVLGVVEPHGDQGGDIARTAAIASGLPQEVAGVQLNRYCASGLEAVNQA
                     AARVRGGWESLIVAGGVESMSRVRMGSGGDPWMMDPETSLASSFIPQGVSADLIATIE
                     GYTRADVDAFAVESHHRAAKAWADGRFDRSVVPVRDRVGAVIIERDEMVRSDASLDSL
                     SGLRPSFAQSGEDGGFDHVALQRYPAVESIAHVHHAGNSSAVVDV"
     gene            979..2121
                     /locus_tag="VNU01_08885"
     CDS             979..2121
                     /locus_tag="VNU01_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HWH32770.1"
                     /translation="MGALLAALVILVTACGSGGEGTSEEGTSAQATENGSEEASETPE
                     FEERELTMASWYAPDTFQGAAINWWIEELAKRTGGAVTVKTYWSESLLGAAEIANGVR
                     DGRVDLGHFCYCYTPGDFPLSTVVEVPFMGDNIGAQVTALNELYKDDEAYRNEWESGG
                     IKLLSHNPVQPALVGAPEPIGGLDWFQGKSIRASGFFAEALKKVGASPVSLPVGDTYE
                     AMQRGTIDAYGSVLLDSLGPLSLLEVGKHVVDPGLGHFVASAWTISQQTWDSMEPELQ
                     AVIEEISAAYPEKLIEASFDIEDKNCQAIKEAGGTLTKLSEADTQRWRELVGESGLDS
                     FVQRASAAGVDGRAFWEKYDALYQEAAEGAHADYVSGVDRCIESLA"
     gene            2308..2940
                     /locus_tag="VNU01_08890"
     CDS             2308..2940
                     /locus_tag="VNU01_08890"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWH32771.1"
                     /translation="MRTITAKDAASLGWPPWAMNLIRAVHAVASAMFVIGAVLLFALI
                     FLTVADVARRKYLGGSIPGAIEFTEVGLVALVFAGMTPAEVARAHVRTPILTSALPAR
                     AASVVRLVGLLIVTAVLAWLTVLTWRLGIQSLRQGEYRFGIAAVPIWPAKMVIPLGIG
                     GMAAEFGLQVALTVHSLIAGRRRGSLVEEVLLTGEVASRVTEQTDDEGAE"
     gene            2937..>3727
                     /locus_tag="VNU01_08895"
     CDS             2937..>3727
                     /locus_tag="VNU01_08895"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWH32772.1"
                     /translation="MSPELLAVLAIMSFLILVLLEVPVALSLLIAGTMGTALLRSPGV
                     AANALAATPYATTAKYALFIIPMYVVLGALISNAGIGEKIYRATSRLVRRLPGGLAAT
                     CVIATSIFSGISGSSAADVATFGRLSVTEMRRNGYDAAYAAAVVAAAGTFAVLIPPSI
                     VLVIYGIMSGESIGAMILAGVVPGAISAGCLVTFVVVKGFIDRSGEATTAPAEAIRAA
                     PAAAPPTPGATPRPSAADGGSAAAQPPSTSGDLIGLLYAAILFVIV"
BASE COUNT          569 a         1272 c         1304 g          582 t
ORIGIN      
        1 cacgtccacc acggccgatg agttcccggc gtggtggacg tgagcgatcg actcgaccgc
       61 cgggtagcgc tgcagggcca cgtggtcgaa ccccccgtcc tcacccgact gggcgaagga
      121 cggtcggagg ccgctcagag agtccagcga cgcgtccgag cgcaccatct cgtcccgctc
      181 gatgatcacc gcgccgacgc ggtcgcggac gggcaccacc gagcggtcga accgtccgtc
      241 ggcccatgcc ttggcggcgc ggtggtgaga ctcgacggcg aacgcgtcga cgtctgcgcg
      301 ggtgtagccc tcgatggtgg cgatcagatc cgcggagacg ccctgtggga tgaacgagga
      361 cgccagggat gtctccgggt ccatcatcca ggggtcgccg ccggaaccca ttcggacgcg
      421 cgacatcgat tcgaccccgc cggcgacgat gagtgactcc cagccgccgc gcacgcgcgc
      481 tgccgcctgg ttgacggctt ccagcccgga ggcgcaatag cggttgagct gcacgccggc
      541 gacctcctgc ggcagtcccg aggcgatcgc agcggtgcgg gcgatgtcgc cgccctggtc
      601 gccatgcggt tccacgacgc ccagcacgat gtcgtcgatg gtgccccgat ccagtgatgg
      661 gttgcgttcg cccagcgcgg cgatgagctg tgtcaccagg gagatcggct tgaccgcatg
      721 gagtgcgccg gtggccttcc cgcgccctcg tggcgtgcgc accgcgtcga agacgactgc
      781 gtccgtcatc gctgtggctc ctttgcctga agtcgtccca atcggatcag cgagtccttg
      841 accaggtcga cggccgatgg tacaccaatc tagcgatcga tcggccgact gcaaaaggcg
      901 gccccgatca aaggaagagc ccgtcgcgac aggatcgtta agaacggaga aatgatgccc
      961 ggtttcaagc ggtacaggat gggcgcgctg ctggccgcgc tggtgatttt ggtgacggcg
     1021 tgcggctcag ggggagaggg cacttccgag gaggggacca gcgctcaggc gactgagaac
     1081 ggctcggagg aggcgtccga gacaccggag ttcgaagagc gcgaactgac gatggccagc
     1141 tggtacgccc ccgacacctt ccagggcgcg gcgatcaact ggtggatcga ggagctggcc
     1201 aagcgcaccg gcggcgcggt caccgtgaag acctactgga gcgagtcgct cctgggcgcg
     1261 gccgagatcg ccaacggcgt ccgcgacggt cgtgtggacc tcggacactt ctgctactgc
     1321 tacaccccgg gtgacttccc cctgtccacc gtggtggagg tgccgttcat gggcgacaac
     1381 atcggagccc aggtgaccgc gctgaacgag ctgtacaagg atgacgaggc gtaccggaac
     1441 gagtgggagt ccggcggcat caagctgctg tcccacaacc cggtccagcc cgcgctcgtc
     1501 ggcgcgccgg agccgatcgg aggcctggac tggttccagg gcaagagcat ccgggccagc
     1561 ggcttcttcg ctgaagccct gaagaaggtc ggagcgagcc cggtatccct cccggtcggc
     1621 gacacctatg aggcgatgca gcgcggcacc atcgacgcat acggctcggt gctgctggac
     1681 tcgctcggcc cgctcagcct gctcgaggtc ggcaagcatg tggtggaccc cggcctgggc
     1741 cacttcgtcg cctccgcctg gaccatctcg cagcagacct gggactccat ggagccggag
     1801 ttgcaggccg tcatcgagga gatcagcgcc gcttaccccg aaaagctgat cgaggcctcg
     1861 ttcgacatcg aggacaagaa ctgccaggcc atcaaggagg ccggcggcac actcaccaag
     1921 ctcagcgagg cggacacgca acgctggcgg gagttggtcg gcgagagcgg tctggactcc
     1981 ttcgtgcagc gcgcgagcgc cgcgggcgtg gacggcaggg cattctggga gaagtacgac
     2041 gctctgtacc aggaggccgc tgagggagcc cacgccgatt acgtcagtgg cgtggaccgg
     2101 tgcatcgagt cgctggcctg actggtcggc gccccggcgc acgtccgcgc ccaggtcggg
     2161 tggcgcgccc cggggctcgt cccccggcac cggggcagcg ccgccacgat cctccgccgc
     2221 gcgcgtcatc gcgcggcgga ggaacggtcc gtgcaccgct gatgccctga cggtgaacga
     2281 agcgtcgctc acacgagagg ccgacccatg cgaactatca ccgccaagga cgccgcgtcc
     2341 ctcgggtggc cgccctgggc gatgaacctg atccgtgcgg tgcatgcagt ggcctcggcc
     2401 atgttcgtca tcggcgcggt cctgttgttc gccctgatct tcctgacggt ggcggatgtc
     2461 gcgcggcgca agtacctggg gggcagcatc cccggtgcga tcgagttcac ggaggtgggc
     2521 ctggtcgccc tggtgttcgc gggcatgaca cctgccgagg tcgcgcgcgc gcacgtgcgc
     2581 acgcccatcc tgaccagcgc actgcccgct cgagcggcgt cggtggtgcg cctcgtcggc
     2641 ttgctgatcg tcaccgccgt gctggcgtgg ctcaccgtgc tcacctggcg gctagggatc
     2701 cagtcgctcc gccagggtga gtaccgcttc ggcatcgcgg ccgtgcccat ctggcccgcc
     2761 aagatggtca tcccgctcgg aatcggcggc atggccgcgg agttcgggct gcaggtggcc
     2821 ctgaccgtgc actcgctcat cgccggaagg cggcgaggct ccctggtcga ggaggtgctg
     2881 ctcaccggcg aagtggcatc gcgggtgacc gagcagaccg acgatgaggg cgcggaatga
     2941 gccccgagct gctggccgtc ctggccatca tgtcgttcct gatcctcgtg ctgctcgagg
     3001 tgcccgtcgc gctgtcgctg ctgattgccg gcacgatggg caccgcgctg ctgcgcagcc
     3061 cgggcgtggc ggccaacgcg ttggccgcca cgccctatgc caccacagcg aaatacgcac
     3121 tgttcatcat cccgatgtac gtggtgctgg gcgcactgat ctccaacgcc ggcatcggcg
     3181 agaagatcta tcgcgccacc agccggctgg tgcggcggct gcctggcggc ctggccgcaa
     3241 cctgcgtgat cgcgacgtcc atcttcagcg gcatctccgg ctccagtgcc gcggacgtcg
     3301 cgaccttcgg ccgtctgtcc gtgacggaga tgcggcgcaa cggctacgac gccgcgtacg
     3361 cggccgccgt cgtcgccgcg gccggaacct tcgcggtcct cataccgccc agcatcgtcc
     3421 tggtgatcta cggcatcatg tccggcgagt cgatcggcgc catgatcctg gctggggtgg
     3481 tcccgggggc catctccgcc ggctgcctgg tcaccttcgt ggtcgtcaaa gggttcatcg
     3541 acaggtccgg cgaggccacg acggcgcctg ccgaggcgat ccgggcggca cccgccgccg
     3601 ctccgccgac gcccggcgcc acgcctcggc cgagcgcggc ggacggcgga agtgcggcgg
     3661 cccagccccc gtccaccagc ggcgacctga tcggcctgct ctatgccgcg atcctgttcg
     3721 tgatcgt
//
LOCUS       DATMKC010000299         3647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_297842, whole genome shotgun sequence.
ACCESSION   DATMKC010000299 DATMKC010000000
VERSION     DATMKC010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297842"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VNU01_08900"
     CDS             complement(<1..305)
                     /locus_tag="VNU01_08900"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWH32773.1"
                     /translation="MALTYRTAGESHGPALFAVVEGLPAGLELVPDDIDVDLARRQLG
                     YGRGGRMKIERDRVQVKTGLRHGRTIGSPLTMEIPNLDFPNWAQDRMAVWAPESEVEP
                     "
     gene            complement(339..3479)
                     /locus_tag="VNU01_08905"
     CDS             complement(339..3479)
                     /locus_tag="VNU01_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWH32774.1"
                     /translation="MTARRTPPNRRLARLSVLLPVLVLVLIAGRVVFHYAAQITVKGE
                     FYDLLGMGDVYDLRWRWGIILALVGLLLTVVLAAPILLIGRSARGAPPVGAAPRPVQP
                     SGPLTEEEIERITRRLETVDLSGLSGGGAAPPDDGHARAVRLAVLGGFAVALVLIGGV
                     MVPGLVAARDTLLAAWQAVPFGTEDPIFGRDVSFFVFTEPAVRELVQIATSALFLAAF
                     ATVATGLALWYTERQRGALLASRAALERTLVFGFGLGGLFLLCLAVLLWLSRYTMTVG
                     GTEVVAGAGAAARDIDIPTRAVGAVVLALLALGLLSLTITAVRRRASITRTGPAALIA
                     LGVWAVTAVALVVIADPWWLVLAVPVGVGLVVAWRNRERPWARRDTPLWAAPAFCAAS
                     ALVLSTLGPAGALLNDAIVLRGSQLQVERENIENTLISTRRAAGIDQAEQVTAEYVQR
                     GVTPADIRRAPASVTSLRFLDIPPTQQACSRTQTLRQFYTCDDVDVDRYMIGGRLRTV
                     FSIGREIDYSRIPDFQPRHFSYTHGYGLITAPVNQIDEAGQPVWIARDIPQTGLNPPL
                     RQPEIYFGAQGNMPWSMVNTEQAVFDQLSNREDVEWCTGEGAPARCGAEGGTGIRVGS
                     GWRRLAVTEYLGGLPYIGGGRRVWNATSGRPAGPDSRLLLFRDLRSRVQEIAPFLRPD
                     SDPWFAAAEGRLWVLLTAYVATDRYPYAARFGEVNYVRQPVTAAMDAYSGQTYIFVTD
                     EDEPMLATWRKVYPDLFRSMDEMDELAPGVRAHLRYGEDVFAFQSAALQRFHVTDPDT
                     FFNGSETWAPTQERYGPGTEGAQIVSPARYTFAVLPGETQERFLLIRSYKPATPNRGI
                     GFSGWLAVSNDPEDFGALTVVNFPTTGRGALVSVETFTGNVGRDPQLSEEIGQRRELV
                     LRGNTIVVPVGNGLLYVQPLYLDTPGDTLPQLWQVVVGVGDGRVFYAPRFQDALAQAL
                     GAGGATGEGDPGGPPATLQELVRQAATEFEAYQRAFGEGNFTEAARRLQRFQRALEQA
                     RRLADQQGEADGAATP"
BASE COUNT          504 a         1326 c         1386 g          431 t
ORIGIN      
        1 ggctccacct cggactcggg cgcccacacc gccatgcggt cctgcgccca gttggggaag
       61 tcgaggttgg ggatctccat cgtgagcggg ctgccgatgg tgcgcccgtg gcgcaggccc
      121 gtcttgacct gcacgcggtc gcgttcgatc ttcatgcgcc cgccccggcc gtagcccagc
      181 tggcggcgcg ccaggtcgac gtcgatgtcg tccggcacga gctcgaggcc ggcgggcagg
      241 ccctccacca ccgcgaagag cgcggggccg tgcgactcgc cggctgtgcg ataggtcagg
      301 gccacggggc ggggagtcta gcgtgcgggc gcgacgccct agggggtggc ggcgccgtcc
      361 gcctcgccct gctggtcggc gagccgccgc gcctgctcga gggcgcgctg gaaccgctgc
      421 aggcgccggg cggcctcggt gaagttgccc tcgccgaagg cccgctggta ggcctcgaac
      481 tcggtggcgg cctggcgcac cagctcctgc agggtggccg gcgggccgcc ggggtcgccc
      541 tcgccggtgg cgcccccggc gcccagggcc tgggcgagcg cgtcctggaa gcgcggcgcg
      601 tagaagacgc ggccgtcgcc gacgcccacc accacctgcc acagctgcgg cagcgtgtcg
      661 ccgggggtgt cgaggtacag cggctgcacg tagagcagcc cgttgccgac ggggaccacg
      721 atggtgttgc cgcgcagcac cagctctcgc cgctgcccga tctcctccga cagctgcggg
      781 tcgcggccga cgttgcccgt gaaggtctcc accgagacca gcgcgccgcg gcccgtggtg
      841 gggaagttga ccacggtgag cgcgccgaag tcctcagggt cgttggagac ggccagccag
      901 cccgagaagc cgatgccccg gttgggcgtc gccggcttgt aggagcggat cagcaggaag
      961 cgctcctggg tctccccggg cagcacggcg aacgtgtagc gcgccgggga cacgatctgc
     1021 gcgccctcgg tgccgggccc gtaccgctcc tgggtgggtg cccaggtctc ggagccgttg
     1081 aagaacgtgt ccgggtcggt cacgtggaag cgctgcagcg ccgcgctctg gaaggcgaag
     1141 acgtcctcgc cgtagcgcag gtgcgcgcgc acgcccgggg cgagctcgtc catctcgtcc
     1201 atgctgcgga acaggtcggg gtagaccttg cgccacgtgg cgagcatcgg ctcgtcctcg
     1261 tcggtgacga agatgtaggt ctggcccgag taggcgtcca tggcggccgt gacgggctgc
     1321 cggacgtagt tgacctcgcc gaagcgcgcc gcgtacgggt agcggtcggt ggccacgtag
     1381 gcggtcagca gcacccacag gcggccctcg gccgccgcga accaggggtc ggagtccggg
     1441 cgcaggaacg gggcgatctc ctgcacccgg gagcgcaggt cgcggaacag cagcaggcgg
     1501 ctgtcgggtc ccgccgggcg gcccgaggtg gcgttccaca cccgccgccc gccgccgatg
     1561 tacggcagcc cgccgaggta ctcggtgacc gccaggcgcc gccagcccga ccccacgcgg
     1621 atgcccgtgc cgccctcggc gccgcagcgg gcaggggcgc cctcgcccgt gcaccactcg
     1681 acgtcctcgc ggttgctcag ctggtcgaac accgcctgct cggtgttcac catcgaccac
     1741 ggcatgttcc cctgggcgcc gaagtagatc tccggctggc gcaggggcgg gttcaggccg
     1801 gtctgcggga tgtcgcgggc gatccagacc ggctggcccg cctcgtcgat ctggttgacc
     1861 ggcgcggtga tcaggccgta gccgtgcgtg tagctgaagt gccgcggctg gaagtcgggg
     1921 atgcggctgt agtcgatctc gcggccgatc gagaagacgg tgcgcaggcg cccgccgatc
     1981 atgtagcggt ccacgtcgac gtcgtcgcag gtgtagaact gccgcagggt ctgcgtgcgc
     2041 gagcaggcct gctgcgtcgg cgggatgtcg aggaaccgca gcgacgtaac cgacgcgggc
     2101 gcgcgccgga tgtccgcggg cgtcaccccc cgctgcacgt actccgcggt cacctgctcg
     2161 gcctggtcga tgccggccgc ccgccgcgtg gagatcagtg tgttctcgat gttctcgcgc
     2221 tccacctgca gctgggagcc ccgcagcacg atcgcgtcgt tcagcagcgc gccggccggg
     2281 cccagcgtgg agagcacgag ggcgctggcg gcgcagaagg ccggcgcggc ccacaggggg
     2341 gtgtcacggc gcgcccacgg ccgctcccgg ttgcgccacg cgaccacgag gcccacgccc
     2401 acgggcacgg ccagcaccag ccaccacggg tcggcgatga cgaccagggc caccgcggtc
     2461 accgcccaga cgccgagcgc gatgagcgcc gcgggaccgg tgcgggtgat ggaggcccgt
     2521 cgccggaccg ccgtgatcgt cagcgacagc agcccgagcg cgagcagcgc cagcacgacg
     2581 gcgccgaccg cgcgggtggg gatgtcgatg tcacgcgccg cggcgccggc gcccgccacc
     2641 acctcggtgc cgcccacggt catcgtgtag cgcgagagcc acagcaggac ggccaggcac
     2701 agcaggaaca ggcccccgag gccgaagccg aacaccaggg tccgctccag cgccgcccgc
     2761 gaggcgagca gcgccccgcg ctgccgctcc gtgtaccaga gcgccagacc ggtggcgacg
     2821 gtcgcgaagg cggccaggaa gagcgcggag gtggcgatct gcaccagctc gcgcacggcc
     2881 ggctccgtga acacgaagaa cgacacgtcc cggccgaaga tggggtcctc ggtgccgaag
     2941 ggcaccgcct gccaggcggc gagcagcgtg tcgcgcgcgg ccaccagccc cggcaccatg
     3001 accccgccga tcagcaccag ggccaccgcg aagccgccga gcaccgccag gcgcaccgcg
     3061 cgggcatggc cgtcgtcggg gggcgcggcg ccgccgcccg acaggccgct caggtcgacg
     3121 gtctccaggc gccgcgtgat gcgctcgatc tcctcctcgg tgaggggtcc cgagggctgg
     3181 accgggcgcg gggcggcccc gacggggggg gcgccccgcg cggagcgccc gatcagcagg
     3241 atcggcgccg cgagcaccac ggtcagcagc aggccgacca gggcgaggat gatcccccag
     3301 cgccagcgca ggtcgtacac gtcgcccatg ccgagcaggt cgtagaactc gcccttcacg
     3361 gtgatctgcg cggcgtagtg gaacaccacc cggccggcga tcagcaccag caccagcacc
     3421 ggcagaagca ccgagaggcg cgccagccgt cggttcgggg gggtgcggcg ggcggtcacg
     3481 agtaggcagg gtatcccgcg cgccgccctc gcggtgcgca tccggtccgg ccggggcgcc
     3541 gccgaccgtt cgcgggtggg cgcgcgggcg ccggcacgag ccgcggcgcc cccgtcggcg
     3601 accggcggtg cacctcatgg gagacgatcc agacgaccgc cgccacc
//
LOCUS       DATMKC010000300         2795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_303476, whole genome shotgun sequence.
ACCESSION   DATMKC010000300 DATMKC010000000
VERSION     DATMKC010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303476"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VNU01_08910"
     CDS             <1..430
                     /locus_tag="VNU01_08910"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HWH32775.1"
                     /translation="EESVALTKERDAQETESLSRALGVGATGKGITGGKPRGTAGALK
                     ELETAQKDRVKRTQRDALDRALVDLAAFYRDVLQLQLGTGTDLVHADLADQAATIARA
                     TTPESTLRRIDAVLACREALDANVAALLAVEAMALALRTG"
     gene            549..621
                     /locus_tag="VNU01_08915"
     tRNA            549..621
                     /locus_tag="VNU01_08915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:582..584,aa:Thr,seq:cgt)
     gene            671..1384
                     /locus_tag="VNU01_08920"
     CDS             671..1384
                     /locus_tag="VNU01_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32776.1"
                     /translation="MRRFEELDWQSTPIGEISLRRRREPALDVDVYEVRLDEDFLMSS
                     LFTVAEVALADLGLAAARGDALDVVVGGLGLGCTARAALADQRVRSLVVVEALPAVIG
                     WHVRELLPLGADLNADPRCSLVEGDFFARVAAGEPFPPQEVDVVLLDVDHSPRHVLHP
                     SHAPFYEVAGLQRLRERLRPGGVFALWSDDPPDDDFLARAGRVFAAAEAVVVPFANHL
                     TGGTSSNTVYVCTLDTDTP"
     gene            1418..1900
                     /locus_tag="VNU01_08925"
     CDS             1418..1900
                     /locus_tag="VNU01_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HWH32777.1"
                     /translation="MDHSTHSGVTAAPEGRALTRLAVSATLHCLTGCAIGEVLGMVIG
                     TAAGWSELQTVALAVGLAFLFGYALGLLPILRSGIGLRAALPIVLAADTLSITTMEVV
                     DNAIMLGIPGAMDAGLTSPLFWGALAVALAVAFVVTVPVNRWLIARGRGHAMVHALHH
                     "
     gene            complement(1975..>2795)
                     /locus_tag="VNU01_08930"
     CDS             complement(1975..>2795)
                     /locus_tag="VNU01_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH32778.1"
                     /translation="PDAAPVRTSGGFTLQPDHDLERLGTADLVAVPAMPRGCAPAAAL
                     QALREAVERGARVMSVCSGAFVLGEAGLLDGRRCTTHWRHSAELAGSFPAAKVDCDVL
                     YVEDGPVLTSAGTAAGIDACLHLVRQEFGARAATALARRMVVPPHRDGGQAQYVQTPV
                     AAAPAQSLQPLLDWVLAHLDQEHTVEALAARALLSPRTFARRFKDETGTTPYAWLSAR
                     RVELASQLLEETDEPVERVAVLAGFGGAAAMRHHFMQVRSTTPQAYRRAFRVPA"
BASE COUNT          337 a         1027 c         1047 g          384 t
ORIGIN      
        1 ggaggagtcg gtcgcgctca ccaaggagcg cgacgcgcag gagaccgagt cgctgtcacg
       61 ggccctcggc gtcggcgcga ccggcaaggg catcaccggc ggcaagccgc gcggcaccgc
      121 cggcgccctc aaggagctgg agacggcgca gaaggaccgg gtcaagcgca cccagcgcga
      181 cgccctggac cgggcgctgg tcgatctcgc ggccttctac cgcgacgtgc tccagctgca
      241 gctcggcacg gggaccgatc tggtgcacgc cgacctggcc gatcaggccg ccaccatcgc
      301 ccgcgccacg accccggagt cgacgctgcg gcggatcgac gcggtgctgg cctgccgcga
      361 ggcgctcgac gcgaacgtcg ccgccctgct ggccgtcgag gcgatggcgc tggcgcttcg
      421 taccggctga ctctcgcgct cgtacttccg cagcgacccg gcgaggcggc agacgaggtt
      481 gctggcgacc tgcgagcggg aagggccggg gtcgggctgg cgcgtctgta gactcgtccg
      541 cgcaccacgc cgccttagct cagtcggcag agcacctcac tcgtaatgag gttgtcatcg
      601 gttcgattcc gataggcggc tcgcctgaag gcccccgtcc gcgggggcct tcgtcgttcc
      661 aggagggcgc atgcgcaggt tcgaggagct ggactggcag tcgacgccga tcggcgagat
      721 cagcctgcgc cggcggcgcg agccggcgct cgacgttgac gtgtacgagg tgcgcctgga
      781 cgaggacttc ctcatgtcca gcctgttcac cgtggccgag gtcgccctgg ccgacctcgg
      841 gctggcggcg gcgcgcggcg acgcgctcga cgtggtggtc ggcgggctgg gcctgggctg
      901 caccgcacgc gccgccctcg ccgaccagcg ggtgcgctcc ctggtggtcg tcgaggcgct
      961 gcccgcggtg atcggctggc acgtccggga gctgctgccc ctcggcgccg acctcaacgc
     1021 cgacccgcgg tgctcgctgg tggagggcga cttcttcgcg cgcgtggccg cgggcgagcc
     1081 gttcccgccg caggaggtcg acgtggtcct gctcgacgtc gaccactcgc cgcggcacgt
     1141 gctgcacccc agccacgcgc ccttctacga ggtcgccggg ctgcagcggc tgcgcgagcg
     1201 gctgcggccc ggcggtgtct tcgcgctgtg gtcggacgac ccgcccgacg acgacttcct
     1261 ggcgcgcgcc ggccgcgtct tcgccgcggc cgaggcggtc gtggtgccct tcgccaacca
     1321 cctcaccggc gggacgtcga gcaacaccgt ctacgtctgc acgctggaca cggatacccc
     1381 ctaggggtat ggtccacgtc gatctcagga ggtggaaatg gaccacagca cccattccgg
     1441 cgtcaccgcg gctcccgagg ggcgggcgct cacgcggctt gcggtctcgg cgacgctgca
     1501 ctgcctgacc ggctgcgcca tcggcgaggt cctcggcatg gtcatcggca ccgcggccgg
     1561 ctggtcggag ctgcagaccg tggcgctggc cgtcgggctg gcgttcctgt tcggctacgc
     1621 gctgggcctg ctgccgatcc tgcgttccgg gatcggcctg cgcgccgcgc tgccgatcgt
     1681 gctggcggcg gacacgctca gcatcacgac catggaggtc gtcgacaacg cgatcatgct
     1741 cgggatcccc ggcgcgatgg acgcgggcct gacgtcgccg ctgttctggg gtgccctggc
     1801 ggtggccctc gccgtcgcct tcgtcgtcac ggtgccggtc aaccgctggc tgatcgcccg
     1861 cgggaggggc cacgccatgg tgcacgcgct gcaccactga cccggcgcaa cgtctgctcg
     1921 caccagcggc gggttgttcc gggggagcag acgttgcgca agcggccggg gggctcaggc
     1981 cgggacgcgg aaggcccgcc ggtaggcctg cggggtcgtg ctgcgcacct gcatgaagtg
     2041 gtgccgcatc gcggccgcgc cgccgaagcc ggccagcacc gcgacccgct cgaccggctc
     2101 gtcggtctcc tccagcagct gggaggccag ctcgacgcgc cgcgcgctga gccaggcgta
     2161 cggcgtcgtg cccgtctcgt ccttgaagcg acgggcgaag gtccgcggcg acagcagcgc
     2221 ccgcgccgcc agcgcctcca cggtgtgctc ctggtccagg tgcgccagca cccagtccag
     2281 cagcggctgc aggctctggg ccggagcagc ggccacgggc gtctggacgt actgcgcctg
     2341 cccgccgtcg cggtgcggcg gcacgaccat ccggcgggcc agcgccgtcg cggcgcgggc
     2401 gccgaactcc tggcggacga ggtgcaggca ggcgtcgatg ccggctgcgg tgccggcgct
     2461 ggtgaggacc gggccgtcct cgacgtagag gacgtcgcag tcgaccttgg cggcggggaa
     2521 gctgccggcc agctcggccg agtggcgcca gtgcgtcgtg cacctgcgcc cgtccagcag
     2581 ccccgcctcg ccgagcacga aggctccgga gcagaccgac atcacccgcg cgccgcgctc
     2641 gaccgcctcc cgcagtgcct gcagcgccgc cgcgggcgcg catccccgcg gcatcgccgg
     2701 caccgcgacg aggtcggcgg tgcccagccg ctccaggtcg tggtcgggct gcagcgtgaa
     2761 gccgccgctc gtgcggaccg gcgccgcgtc ggggg
//
LOCUS       DATMKC010000301         3837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_32818, whole genome shotgun sequence.
ACCESSION   DATMKC010000301 DATMKC010000000
VERSION     DATMKC010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32818"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNU01_08935"
     CDS             <1..278
                     /locus_tag="VNU01_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HWH32779.1"
                     /translation="VAVCGGAGDSLVGAALGAGADCYVTGDLRHHVALDALTMGMALI
                     DAGHYATEAAALPHLRATLAEAASRHGLSARLVASEVRTEPWSDWRA"
     gene            296..1030
                     /locus_tag="VNU01_08940"
     CDS             296..1030
                     /locus_tag="VNU01_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HWH32780.1"
                     /translation="MIPASTAEQRRLYDLQQVDSAIRKLQVRRSNLPEQQALDENAET
                     LAAITKEFADARERLDHLTRQQSRLEQEIATLDSRRKSEEGRMYSGLITSEKESEALR
                     NELHSIKGRKRDLEDQLIEVMEELEGLESLTTTLKERYDELTTKVAELTEARDHAASD
                     IDAEIAQRHAEREALAADVPEEIRGHYEELRDKKQGVAVAELEGRSCGGCRLELTAIE
                     LEELKEDVTKGLAKCPQCGRILVLAA"
     gene            complement(1072..2112)
                     /locus_tag="VNU01_08945"
     CDS             complement(1072..2112)
                     /locus_tag="VNU01_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWH32781.1"
                     /translation="MRAARFDQGSRKLTLEVVPVPQPGPLEALVRVQACGICLSDVHL
                     IDGSLPGMLPVLTPGHEAAGVIEALGEQVQGWRVGQRVAMAGGKPCFSCANCSRGRLD
                     ECLQFMIMGFAYDGAWAEYVVVPAMALTQVPDHVPIEQAAILADAVSTPYAGLVERAG
                     LRAGETVGLWGIGGLGVHAVQIARMVGAATIVAVDPNAAARERALEMGADHALDPTTQ
                     DVQAEVMAITRGLGLDLAVDLYGANAVLAQGASCLGRHGRLLMVGLSLEPIQLGPGVL
                     LGVQSQSILGHLGYAKRHLDELVDLVASGRLDVSKSVTEVMPLSEVARGVERLASKEG
                     NPVRLVVDPTLG"
     gene            2246..>3837
                     /locus_tag="VNU01_08950"
     CDS             2246..>3837
                     /locus_tag="VNU01_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HWH32782.1"
                     /translation="MGDDMRSLREQVRPDDREQLRIALVMSGGSSLAIWIGGVSAEVH
                     RLTQTAPGDATGYGALLDITRSEARMDVIAGTSAGGLNGAFLALAAARTADLSPLAEL
                     WATRGAASELLRPALEADPPSLLRGDGYFLPELRRALQGIWDAGRRYVPPDVMPLHLI
                     MTTTVLAGVPKTFVDDFGNRLHEVEHRATFEFLRDARTVPAGEPVDARSRARRDPFGD
                     PAVVGRLALAARSTASFPGAFEPSFVPIGAGHDELHPDMSQVIDLAASGFVLDGGVLV
                     NRPVDLALRAVFRQPAAAQVRRALIFVEPDPAGELALEPDRYDRPPSLRQVMVDSLTT
                     LPRAQSVAGALESLLEHNQRVRERRAVRLRLGGSPDGATAGDAPAADVADLAGALFPR
                     YVEQRREAALRTVFALARHDHLLGVGVLPGGVPAWSELEFAAAFRRARELPFVPDSMG
                     DDPAERWGVDFAEHLAYLAVDVLRRAIWLVPADQSDLRRRVRERRAELHETLRSLHGL
                     REEDRRFWQVLLAELLPPPASAS"
BASE COUNT          527 a         1437 c         1389 g          484 t
ORIGIN      
        1 ccgtggcggt gtgcggtggc gcgggcgact cgctcgtcgg cgcggcgctg ggcgccggcg
       61 cggactgcta cgtcaccggg gacctgcgcc accacgtcgc cctggacgcg ctgaccatgg
      121 gcatggcgct gatcgacgcc ggccactacg ccaccgaggc ggcggcgctg ccgcacctgc
      181 gcgccacgct cgccgaggcc gcgtcgcggc acggcctgtc ggctcggctg gtagcgtcgg
      241 aggtgcgcac cgaaccctgg tccgactggc gggcctgaca gcaggaggaa cattcttgat
      301 cccggcgagc accgcggagc agcgcaggct gtacgacctc cagcaggtgg actccgccat
      361 ccgcaagctc caggtgcgcc ggtcgaacct gcccgagcag caggcgctgg acgagaacgc
      421 cgagacgctg gccgcgatca ccaaggagtt cgccgacgcc cgcgagcgcc tggaccacct
      481 cacgcggcag cagtcgcggc tggagcagga gatcgccacg ctcgactcgc gccgcaagtc
      541 ggaggagggc cgcatgtact ccggcctgat cacctccgag aaggagagcg aggcgctgcg
      601 caacgagctg cacagcatca aggggcgcaa gcgcgacctg gaggaccagc tcatcgaggt
      661 gatggaggag ctggagggcc tggagtcgct caccaccacg ctcaaggagc gctacgacga
      721 gctcaccacc aaggtggccg agctcaccga ggcgcgcgac cacgccgcct ccgacatcga
      781 cgcggagatc gcgcagcgcc acgccgagcg cgaagccctg gccgccgacg tgcccgagga
      841 gatccgcggc cactacgagg aactgcgcga caagaagcag ggcgtggccg tcgcggagct
      901 ggaaggccgc tcctgcggcg gctgccgcct ggagctgacg gccatcgagc tggaagagct
      961 caaggaggac gtcaccaagg gcttggccaa gtgcccgcag tgcgggcgca tcctggtgct
     1021 cgcggcctag ctgcggttcc caggacattg ccgcccggtc aaccgggcgt ctcagcccag
     1081 agtgggatcc acgacgagtc gcaccgggtt gccctccttg ctcgccaggc gctccacgcc
     1141 ccgggccacc tcggacagcg gcatgacctc ggtgaccgac ttggacacgt ccaggcgtcc
     1201 ggaggccacc aggtcgacca gctcgtcgag gtggcgcttg gcgtaaccca ggtggcccag
     1261 gatgctctgc gactgcacgc ccaggaggac cccggggccc aactggatcg gctccagcga
     1321 cagtcccacc atgagcagcc ggccgtggcg gcccaggcag ctcgcgccct gtgccagcac
     1381 ggcgttcgcc ccgtacaggt cgacggcgag gtccaggccc aggccgcggg tgatcgccat
     1441 gacttcggct tggacgtcct gcgtggtggg gtccagcgcg tggtcggcgc ccatctccag
     1501 ggcgcgctcg cgggcggccg cgttggggtc gaccgcgacg atcgtcgccg cgcccaccat
     1561 gcgcgcgatc tgcaccgcgt gcaccccgag gccgccgatg ccccacaggc ccaccgtctc
     1621 gcccgccctg aggccggcgc gctccaccag tcccgcgtac ggcgtggaga cggcgtcggc
     1681 caggatcgcc gcctgctcga tcggcacgtg gtccggcacc tgcgtcagcg ccatcgccgg
     1741 caccaccacg tactcggccc acgcgccgtc gtaggcgaag cccatgatca tgaactgcag
     1801 gcactcgtcc agccggccgc gcgagcagtt ggcgcacgag aagcagggct tgccgcccgc
     1861 catcgccacc cgctggccca cgcgccagcc ctgcacctgc tcgcccagcg cctcgatcac
     1921 gccggccgcc tcgtgccccg gcgtgagcac gggcagcatg cccggcagcg agccgtcgat
     1981 gaggtgcacg tcggacaggc agatgccgca cgcctgcacg cgcaccagcg cctccagcgg
     2041 gccgggctgg gggaccggca ccacctccag tgtgagcttc ctcgacccct ggtcgaaccg
     2101 tgccgcccgc atggtgtcca cgtcccgctc ctgttccgct ccgccggatg ccggcccgca
     2161 ggctagaacg ccctgacgcg cgtcgcccga atcgcgtggc gcgttggtta gcatccgtcc
     2221 gacgtcgcga ggggagtggc acgccatggg ggacgacatg cgcagcctgc gcgagcaggt
     2281 gcgaccggac gaccgcgagc aattgcgcat cgcgctggtg atgagcggcg gcagcagcct
     2341 cgcgatctgg atcggcggcg tgagcgccga ggtgcatcgc ctcacccaga ccgcgccggg
     2401 ggacgcgacc ggctacggcg cgctgctgga catcacgcgg tccgaggcgc ggatggacgt
     2461 catcgcaggc accagcgcgg gcggcctgaa cggtgcgttc ctggcgctcg ccgccgcgcg
     2521 caccgccgac ctgtcgccgc tcgcggagct gtgggcgacc cggggcgcgg ccagcgagct
     2581 gctgcgcccc gcgctggagg ccgacccgcc ctcgctgctg cgcggcgacg gctacttcct
     2641 gccggagctg cgccgggcgc tccagggcat ctgggacgcc ggccgccggt acgtgccacc
     2701 cgacgtgatg ccgctgcacc tgatcatgac cacgaccgtg ctcgccggtg tgcccaagac
     2761 gttcgtggac gacttcggca accgcctgca cgaggtggag catcgcgcga cgttcgagtt
     2821 cctccgcgac gcgcgcaccg tgcccgccgg cgagccggtg gacgcccgct cccgcgcgcg
     2881 gcgcgacccg ttcggcgacc ccgcggtcgt cgggcgcctg gcgctggccg cccgcagcac
     2941 cgcgagcttc ccgggcgcct tcgaacccag cttcgtgccg atcggcgccg gccacgacga
     3001 gctgcacccg gacatgagcc aagtgatcga cctcgcggcg agcgggttcg tgctcgacgg
     3061 cggcgtcctg gtgaaccgtc cggtcgacct ggcgctgcgc gcggtcttcc gccagcccgc
     3121 cgccgcccag gtgcgccgcg ccctgatctt cgtggagccg gacccggcgg gggagctcgc
     3181 gctggagccg gaccgctacg accggccgcc ttcgctgcgc caggtgatgg tcgacagcct
     3241 caccacgctg cccagggccc agtccgtggc gggagcgctg gagtcgctgc tggagcacaa
     3301 ccagcgcgtg cgcgagcgcc gggccgtgcg gctgcgcctg ggtggaagcc ccgacggcgc
     3361 cacggcgggc gatgcgccgg cggccgacgt ggccgacctc gccggagcgc tgttcccccg
     3421 ctacgtcgag cagcggcgcg aagccgcgct gcgcaccgtg ttcgcgttgg cccggcacga
     3481 ccacttgctc ggcgtcggcg tcctgcccgg cggcgtgccg gcgtggtcgg agctggagtt
     3541 cgccgcggcg ttccggcgcg cgcgggagct gccgttcgtc cccgactcga tgggcgacga
     3601 cccggcagag cgttggggcg tggacttcgc cgagcacctg gcgtacctgg ccgtcgacgt
     3661 gctcaggcgg gcgatctggc tggtcccggc ggaccaaagc gacctgcgtc gccgtgtgcg
     3721 ggagcgccgc gccgagctgc acgagacgct gcggtccctg cacggcctgc gcgaggagga
     3781 ccggcgcttc tggcaggtcc tgctggcgga gctgctgccg ccgccggcgt cggcgtc
//
LOCUS       DATMKC010000302         5692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_23858, whole genome shotgun sequence.
ACCESSION   DATMKC010000302 DATMKC010000000
VERSION     DATMKC010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23858"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..781)
                     /locus_tag="VNU01_08955"
     CDS             complement(164..781)
                     /locus_tag="VNU01_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWH32783.1"
                     /translation="MGTLILIRHGQSVWNAENRFTGWVDTPLSDLGREEAHRAGRRLA
                     AEGVRVDRAFTSMLRRAIETGRAVLDELGQPDLEQVQAWELNERFYGALTGRDKAQTA
                     EEFGADQVKVWRRSYDIPPPGGESLKDTADRTLPYFRREILPAASADQTVLVAAHGNS
                     LRSIVKDLDGLDDEAIVAVEIATAVPLVYTLGEDGSVKDKRVLDS"
     gene            872..1867
                     /locus_tag="VNU01_08960"
     CDS             872..1867
                     /locus_tag="VNU01_08960"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HWH32784.1"
                     /translation="MASDETHVEVAGERVRVSSPGKVMFPAQGWTKMDLVEHFLATRE
                     GALRNVYGRPTVLKRWNKGVGAEPVFQKRAPKQSAHTYATISFPSGRTADLLVPRHAA
                     DVVWMAQLNCIDFNPWPVRAEDTDHPDELRVDLDPTPDTGFEVVREVALVCGDVLREH
                     GLTGWPKTSGNRGIHIFVRIAPEWSFTLVRRAALALAREVERRVPGKATSAWWKEERH
                     GVFVDYNQNARDRTVASAYSVRQTGLVSAPLRWDEVADVDPRDFPMDRYAATRYAVVG
                     DLTDGIDEAPGRLDQLLELSRRDEAEGLGDAPWPPHFPKMEGEPPRVQPSRRRAP"
     gene            1905..3764
                     /locus_tag="VNU01_08965"
     CDS             1905..3764
                     /locus_tag="VNU01_08965"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32785.1"
                     /translation="MRARVVMRAFLVTLLAAYATAYVPAAMGEAEQPASGVAADDGSG
                     AVLRQDPPPEGRRVERLAGTDRVGTAVAVARATYARATVALLASADGYADALVAAPYA
                     ADLGAPLLLTPRDRLPEAVADVLADLGVRRVTLLGGPAAISEEVADTLRRTYRVGRVA
                     GADRYGTAARVALLVPASDEVFLATGAGFADGLAVGPLAAAAGAPILLTLPDRLPEVT
                     AQRLAERGPRRVTLVGGSGAIAPEVESSLRADGWQVRRLSGASRHATSVAVADAAIAE
                     GASPAEVWLADGERFPDALAAGPAVAARGGVLLLVDGRRLGAATPVGDWLRARKEALR
                     RIVVLGGPGVMAPDAAAQIEAILAGRQLPRGGRGLLPEHRLVALYGHSSTPALGSMGE
                     QPPEQAADRAVAAAQPYEQGGKPVLPTFEFIATVATAGPGTSGLYRAPTDPAVIQRSL
                     DAIRRVRGYLVLDIQPGRSDFLTESKRYEHFLAQPDVGLALDPEWRMAPDQRPGEAVG
                     SVHASEINAVSAWLADLVARHALPEKLLLLHQFQDRMIRERHMLEPREGLAITVQMDG
                     FGSRSQKLATYARTRTGPPLHNGFKLFYDEDVNLYQPHEVLALDPVPDFVSYQ"
     gene            complement(3751..4767)
                     /locus_tag="VNU01_08970"
     CDS             complement(3751..4767)
                     /locus_tag="VNU01_08970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH32786.1"
                     /translation="MNVWDEPVRGVRASPGLTRLSGIESMRGFLERRFPAPPISRLTG
                     LRPADAGLTHSVFTLPVTRWLLDATGHLMPSTAALVSDAPLGAAFGNSLPAGKVCTTS
                     ELSISYLRPAGMDAERLIAQAEVIKAGRTTGLTEARITDAQGRLLAHATSRLVVLDVP
                     VFDDAPLPGPDDAPPTPDPWRREAEGAVADPAAFAGLTGREIFTGWLSGALPRPPVSH
                     LTGIRPQEIGDDAVRWSMPASEWLCSPAPYLYGGATAMLADAALTSAVMPALPADVAF
                     RPLDLKLRFLRPVWPGSGDLTAEGRLVHHGKSIAVTRGEVRAADGKVAILAESSLQLR
                     PTGS"
     gene            complement(4764..>5692)
                     /locus_tag="VNU01_08975"
     CDS             complement(4764..>5692)
                     /locus_tag="VNU01_08975"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWH32787.1"
                     /translation="FRMVAWRDPLRIDSRNQRPLLRYFPELEPVVALLPPDAVVDGEV
                     VVVLDGRTNFDALQNRLHPAESRVRMLSAETPARLIAFDCLAAGGRDLRGAPFSERRA
                     ALETLFAAELDGSPWHLTPSTSDVEEAGRWFRDFESAGCDGIVAKRLDGAYAEGERAM
                     VKVKHRRTVDCVIGGYREHKDGGKIGSLLLGLYNGAGQLHFIGHCSGFGDADRVAILE
                     RLLPLVRQDSFGDAARKPGQESRWSGGKDMSWTAVAPELVCEVSYDQLTGERFRHATR
                     FERWRPDKAPEQCGMDQLERPDGPGFEDVVRA"
BASE COUNT          715 a         2134 c         2116 g          727 t
ORIGIN      
        1 ctcccacacc gccgggtccg tggggcccac ctcccgccag tcgcagccgc cctggcagcg
       61 gcgcagcacg gcgtccggga cgcgggtgtg gtcgttgaac ggcaccacct gcgtgcagtg
      121 cgcacaacgg taggtggtcc cgcggcggac ggtcatgccg ccgtcacgag tccaggaccc
      181 gcttgtcctt gacggagccg tcctcgccca gggtgtagac cagcggcacg gcggtcgcga
      241 tctccaccgc cacgatcgcc tcgtcgtcca gcccgtcgag gtccttcacg atggagcgca
      301 gcgagttgcc gtgcgcggcc acgagcaccg tctggtcggc gctcgcggcc ggcaggatct
      361 ccctccggaa gtacggcagc gtgcggtcgg cggtgtcctt caggctctcg ccaccgggcg
      421 gggggatgtc gtagctgcgc cgccacacct tgacctggtc ggcgccgaac tcctcggcgg
      481 tctgcgcctt gtccctcccg gtgagcgcgc cgtagaagcg ctcgttgagc tcccacgcct
      541 gcacttgctc caggtccggc tggccaagct cgtccagcac cgcgcgcccg gtctcgatcg
      601 cccggcgcag catggacgtg aacgcgcggt ccacgcgaac cccttcggcg gccaggcgcc
      661 gccccgcgcg gtgggcctcc tcccggccca ggtcggacag cggcgtgtcc acccagcccg
      721 tgaagcggtt ctcggcgttc cacacggact ggccgtggcg gatgaggatg agcgtgccca
      781 cgcgagcggt tctccctggt cggtccgacg atgcgccaca ttgcagcacg ccgccgcctg
      841 cacgcgcgaa tgcggggcta ggctgaatcg catggcgagc gacgagaccc acgtcgaggt
      901 ggcgggcgaa cgcgtgcggg tgtccagccc cggcaaggtg atgttccccg cccagggctg
      961 gaccaagatg gacctcgtcg agcacttcct ggccacccgc gagggcgcgc tgcgcaacgt
     1021 ctacggccgg cccacggtgc tgaagcgctg gaacaagggc gtgggcgccg agccggtgtt
     1081 ccagaagcgg gcacccaagc agagcgcgca cacctacgcc acgatcagct tcccgtcggg
     1141 gcgcaccgcg gacctgctgg tcccgcgtca cgccgccgac gtcgtctgga tggcccagct
     1201 caactgcatc gacttcaacc cgtggccggt gcgcgcggag gacaccgacc accccgacga
     1261 gctgcgcgtc gacctggacc ccacaccgga caccggtttc gaggtggtac gggaggtcgc
     1321 gctggtgtgc ggcgacgtgc tgcgcgagca cggcctgacc ggctggccca agacctccgg
     1381 caaccgcggc attcacatct tcgtgcgcat cgcgccggaa tggtccttca ccctggtgcg
     1441 gcgcgccgcc ctggcgctcg cgcgagaggt ggagcggcgc gtgcccggca aggcgacgtc
     1501 ggcgtggtgg aaggaggagc gccacggcgt gttcgtcgac tacaaccaga acgcgcgcga
     1561 ccgcaccgtg gcgtccgcct acagcgtgcg ccagaccggc ctggtgagcg cgccgctgcg
     1621 ctgggacgag gtcgccgacg tcgacccgcg cgacttcccg atggaccgct acgccgccac
     1681 gcgctacgcc gtcgtgggcg acctcacgga cggcatcgac gaggcgccgg gccgcctgga
     1741 ccagctgctg gagctgtcca ggcgcgacga ggccgagggc ctgggcgacg cgccgtggcc
     1801 gccgcacttc cccaagatgg agggcgagcc gccgcgcgtg cagccctcgc gccgccgcgc
     1861 cccgtaggct gctgtcgcga cgggaaacgg caaggggagg cgcggtgcgg gctcgggtgg
     1921 tgatgcgcgc gttcctggtg acgctgctgg ccgcctacgc caccgcctac gtgcccgccg
     1981 ccatgggcga ggccgagcag cccgcgtccg gtgtcgcggc cgacgacggc tccggcgccg
     2041 tactgcgaca ggacccgccg ccggaaggcc ggcgcgtgga gcgcctggcg ggcaccgatc
     2101 gcgtgggcac cgccgtcgcg gtggcgcgcg ccacctacgc gcgggcgacg gtcgcgctgc
     2161 tggcgagcgc ggacggctac gccgacgcgc tggtcgccgc gccctacgcc gccgacctcg
     2221 gcgccccgct gctgctgaca ccgcgcgacc gcctgccgga ggcggtggcc gacgtgctgg
     2281 ccgacctggg cgtgcgccgc gtgacgctgc tgggcggccc ggccgcgatc tccgaggagg
     2341 tcgcggacac gctgcgccgg acctaccgcg tcggccgcgt ggccggcgcg gaccgctacg
     2401 ggaccgcggc gcgggtcgcg ctgctggtgc cggcctccga cgaggtgttc ctggccaccg
     2461 gcgccggctt cgccgacggc ctggccgtgg ggccgctcgc ggcggcggcc ggcgcgccga
     2521 tcctgctgac cctgcccgac cggctgccgg aggtcacggc gcagcggctg gccgagcgcg
     2581 ggccgcggcg cgtgacgctg gtcggcggca gcggggcgat cgcgccggag gtggagtcgt
     2641 cgctgcgggc ggacggctgg caggtccgcc gcctgtcggg tgcgtcccgc cacgccacct
     2701 ccgtggccgt ggccgacgcc gccatcgccg agggtgcgag tcccgcggag gtgtggctgg
     2761 ccgacggcga gcgcttcccg gacgccctgg cggccggccc ggccgtcgcg gcgcgcggcg
     2821 gcgtgctgct gctggtcgac ggccggcgcc tgggcgccgc gacgccggtg ggtgactggc
     2881 tgcgggcgcg caaggaggcg ctgcggcgca tcgtggtgct gggcggcccc ggcgtgatgg
     2941 cccccgacgc cgccgcccag atcgaggcca tcctggccgg ccggcagctg ccgcgcgggg
     3001 gccgcggcct gctgccggag caccggctgg tggccctgta cgggcacagc tccacgccgg
     3061 cgctggggtc catgggcgag cagccgccgg agcaggcggc cgaccgggcc gtcgccgccg
     3121 cgcagcccta cgagcagggc ggcaagccgg tgctgcccac cttcgagttc atcgccaccg
     3181 tggccacggc ggggcccggg acgtccgggc tgtaccgggc gcccaccgac cccgcggtca
     3241 tccagcgctc cctggacgcg atccggcgcg tgcgcggcta cctggtcctg gacatccagc
     3301 ccggccgctc ggacttcctc acggagtcca agcgctacga gcacttcctt gcccagcccg
     3361 acgtcggcct ggctctggac ccggagtggc gcatggcgcc cgaccagcgt cccggcgagg
     3421 cggtcggctc cgtgcacgcc tcggagatca acgccgtgtc ggcgtggctg gcggacctgg
     3481 tggcccgcca cgcgctgccg gagaagctgc tgctgctgca ccagttccag gaccgcatga
     3541 tccgcgagcg ccacatgctg gagccgcgcg agggcctggc gatcacggtg cagatggacg
     3601 gcttcggcag ccgctcgcag aagctggcga cgtacgcccg cacgcgcacc gggccgccgt
     3661 tgcacaacgg cttcaagctg ttctacgacg aggacgtgaa cctgtaccag ccgcacgagg
     3721 tgctggccct ggacccggtc ccggacttcg tcagctacca gtagggcgca gctgcagcga
     3781 ggactccgcc aggatggcca ccttgccatc ggcggcgcgc acctcgccgc gggtcacggc
     3841 gatgctcttg ccgtggtgga ccaggcgccc ctccgccgtc agatcgccgc tgccgggcca
     3901 caccggccgc aggaagcgca gcttgaggtc caggggccgg aacgccacgt cggccggaag
     3961 tgcgggcatc acggcgctgg tgagcgcggc gtcggccagc atggcggtcg caccgccgta
     4021 caggtacggg gcgggggagc acagccactc cgaggcgggc atgctccagc gcaccgcgtc
     4081 gtcgccgatc tcctgcggcc ggatgccggt caggtgggac accggcggcc gcggcagcgc
     4141 gccgctcagc cagccggtga agatctcccg tccggtcagg ccggcgaacg ccgccgggtc
     4201 ggcgacggcg ccctccgcct cgcgccgcca cgggtcgggc gtcggcggcg cgtcgtccgg
     4261 ccctggcagc ggcgcatcgt cgaacacggg cacgtccagc accaccaggc ggctggtcgc
     4321 gtgcgccagc agccgtcctt gagcatcggt gatccgcgcc tccgtgagcc cggtcgtgcg
     4381 tcccgccttg atcacctccg cctgggcgat caggcgctcg gcgtccatgc ccgcgggccg
     4441 caggtagctg atggacagct cgctggtggt gcacaccttg ccggcgggaa gggaattgcc
     4501 gaacgccgcg cccagcggcg cgtcgctgac cagcgccgcg gtcgacggca tcaggtggcc
     4561 ggtggcgtcc agcagccagc gcgtcaccgg cagcgtgaac accgagtgcg tcaggccggc
     4621 gtcggcgggc cgcagccccg tcaggcggct gatcggcggg gccgggaagc gccgctccag
     4681 gaagccgcgc atcgactcga tgccggagag ccgtgtcagg ccggggctgg cccgcacgcc
     4741 gcgcaccggc tcgtcccaca cgttcacgcg cgtaccacgt cctcgaaccc ggggccgtcc
     4801 ggccgctcca gctggtccat gccgcactgc tcgggcgcct tgtccgggcg ccagcgttcg
     4861 aaacgcgtcg cgtggcggaa acgttccccg gtgagctggt cgtagctgac ctcgcacacc
     4921 agctcgggcg cgaccgcggt ccacgacatg tccttgccgc cgctccagcg gctctcctgg
     4981 ccgggcttgc gcgcggcgtc gccgaagctg tcctggcgca ccagaggcag caggcgctcc
     5041 aggatcgcca cccggtcggc gtcgccgaag ccggagcagt gcccgatgaa gtgcagctgg
     5101 ccggcgccgt tgtacagccc cagcagcagc gagccgatct tgccgccgtc cttgtgctcg
     5161 cggtagccgc cgatcacgca gtccacggtg cggcggtgct tgaccttcac catggcgcgc
     5221 tcgccctcgg cgtaggcgcc gtccagcctc ttggccacga tgccgtcaca gccggccgac
     5281 tcgaagtcgc ggaaccagcg cccggcctcc tccacgtcgg aggtcgacgg cgtgaggtgc
     5341 cacggggagc cgtcgagctc cgcggcgaac agcgtttcca gggccgcgcg gcgctcgctg
     5401 aacggggcgc cccgcaggtc gcggccgccc gcggccaggc agtcgaacgc gatcaggcgc
     5461 gcgggcgtct ccgcggacag catgcgcacg cgcgactcgg ccgggtgcag ccggttctgc
     5521 agcgcgtcga agttcgtcct gccgtccagg accacgacca cttcgccgtc cacgacggcg
     5581 tccgggggca gcagcgcgac gaccggctcc agctcgggga agtagcgcag gagcgggcgc
     5641 tggttgcggc tgtcgatgcg cagcgggtcg cgccaggcca ccatgcggaa ac
//
LOCUS       DATMKC010000303         5284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_111542, whole genome shotgun sequence.
ACCESSION   DATMKC010000303 DATMKC010000000
VERSION     DATMKC010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111542"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..643
                     /locus_tag="VNU01_08980"
     CDS             185..643
                     /locus_tag="VNU01_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWH32788.1"
                     /translation="MVFRSHARLTPEALSPAAALAFVADASAGGTVLFTGTVRDHADG
                     REVASLEYEAYAEVAQPRMEELAAEAHRRWPALAAAWLEHRVGALEIGEAAVVVAASA
                     PHRDDAFAAARWLIDTLKETVPIWKRELWADGGAHWPGTPDPVGGTVAGD"
     gene            643..1635
                     /locus_tag="VNU01_08985"
     CDS             643..1635
                     /locus_tag="VNU01_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWH32789.1"
                     /translation="MGRDLAIDLGTANTLVWARGRGIVLNEPSVIAVNARSGAVLAMG
                     RDAYGMVGRTPGHIVTERPLRGGAITDFGTTSRMIELVLRRCGVTRWSKPKVLICVPS
                     AITHVERRAVEEAAKQAGASHAYLMEQPMAAAIGARLPVHEPMGNMVLDVGGGTSEVA
                     VISLGGVVVAKAVRVGGFDIDAAIQAFIRNHYAVAIGERTSEELKMAIGSAYPQSEEP
                     KAEIRGRELATGLPKTVVIGSDEIREAIDEQVTQIVGAVTDTLADCPPELTQDVLRSG
                     IWLVGGGALLQGLDARIAQETQVKVNVVDAPLEAVVVGAGTTLESFDNLKHLFV"
     gene            1658..4282
                     /locus_tag="VNU01_08990"
     CDS             1658..4282
                     /locus_tag="VNU01_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32790.1"
                     /translation="MTAGLSTPLHLAASLLALSAAVGLAVVGIVGLRARRPGSLLVTV
                     GALLLAVGHGLAGALVTQAADVAALLVPLPPLPPTLFAAAGGALAVIRLLPRGGAAAL
                     AGVGLGLLGAAELVSRSSATAGAVLLVLGALGVGVWLWAGSARRLLAKVATAFVALLL
                     ALIVLVAGILSGQASGQLVDEELARLEDLSVRLADEIGSAWPQDAIAAARPLRNSGQL
                     LLGIAPDDRDRLADINDLSFRQQDFFALLGADGRVVTAYSDDPALLSGSFTLALTGAQ
                     AVDRILEGRDEDGGLVTVGGKLVALGAVKLAAQDARAEEPPIGVLLTGRVVDEVWAAQ
                     TARAQGAGIVVEVAEAPSVASPELPVAGTDPARDVIRATTQGEGTGVLTGDGRSAYAA
                     SAPIRDPNTATLVGRIATVRAAEVIAEVEREQVSSLFVIAVLGALAAGLVGAAITRRL
                     VAPVRRLTEAAEAVRAGRLDTRAQVDTPDEIGRLGRTFDEMTTSLAAQSAELVEAAEV
                     QSRLRARLEAMTDSMGDALVAVDAAGEIVTFNPAAERLTGRRAADVAGGPLDRALRLA
                     ATPDTPVAVVLGAPDSEDQVQVQVLLRGQRGRPVQAAVTASPVRDADGKVLGRVLVLR
                     DMTREAEVERMKAEFLANVSHELRTPLTPIKGYADILARRDVDKDKARRYAQQILEST
                     ALLERIVGMIVDFAALDSGQVELAREPVRLSDLVAETLGEWRARHPKREFKRRVAKTL
                     PAVLADPAMLRRALDELLDNAVKFSPRGEPVSVTAAVEEDRAGRWVRLSVRDRGVGID
                     PETAANVFSDFYQVDASETRQFGGLGLGLALVRRIVEGVGGRAAVDSEPDEGTEVHLL
                     LPAAEDTGRKPRGTRSSG"
     gene            4332..>5284
                     /locus_tag="VNU01_08995"
     CDS             4332..>5284
                     /locus_tag="VNU01_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32791.1"
                     /translation="MKAGLAALLSLAIALALAGCSTARLGGAAGRLTQGETVMVQQGD
                     GWRALAAGDSVPDGARVRTGLETARLELQRGELWLGHSATVRLRGDDVEVERGEIVLH
                     SQGARASWQAVEVTGEGVFRLAPGASPRVGVYAGEVTVARPGEQRRLPALRQLGLAAR
                     RLPAEPDPLYYDTGDDWDRELLPKAISFDEEVSRLARGIDREYGTDPQPADFYTSFTA
                     VRPETVPVLAATARRADADGSFGPPSDALVTLFVSSAVAAAQGLAIEDAVRRVADKRA
                     VGARWGLIAVEEDIDVQRLAAAVDLARERRQIVAPDDERTIG"
BASE COUNT          616 a         1953 c         2049 g          666 t
ORIGIN      
        1 cggcaggcac gtcgcgctcg ggcccgtgcg gacgtccaag cgcaccctgg cggccgcggg
       61 gctcggcgcc gccaccctca cgggcgtgct gctgcgccgg ctgcgccgcg cctgacgcgc
      121 tgcggctgat cggccaggcc cgcccgcact gcaggccact gcgcgccgtg gcaagctgcc
      181 ggtgatggtc ttccgctccc atgcgcgcct cacccccgag gcactgtcgc ccgccgccgc
      241 gctggcgttc gtggccgacg cgtccgcggg cggcaccgtg ctgttcaccg gcacggtgcg
      301 cgaccacgcg gatgggcgcg aggtcgcctc gctggagtac gaggcgtacg ccgaggtggc
      361 gcagccgcgc atggaggagc tggcggccga ggcgcaccgc cggtggccgg cgctggccgc
      421 cgcctggctg gagcaccgcg tcggcgccct ggagatcggc gaggccgcgg tggtcgtcgc
      481 ggcgtcggcg ccccaccgcg acgacgcgtt cgccgccgcg cgctggctga tcgacacgct
      541 caaggagacc gtgcccattt ggaagcgtga gctgtgggcg gacggtggcg cgcactggcc
      601 cggcacgccg gaccccgtcg gcggcaccgt ggcgggggac tagtgggtcg cgacctcgcc
      661 atcgacctgg gcaccgccaa cacgctggtg tgggcgcgcg gccgcggcat cgtgctgaac
      721 gagccgagcg tcatcgccgt caacgcccgc tccggcgccg tgctggccat gggacgcgac
      781 gcctacggca tggtcggccg cacgccgggc cacatcgtca cggagcggcc gctgcgcggc
      841 ggcgcgatca ccgacttcgg caccacgtcg cgcatgatcg agctggtgct gcgccggtgc
      901 ggcgtcacgc gctggagcaa gccgaaggtg ctgatctgcg tgcccagcgc gatcacgcac
      961 gtggagcgcc gggccgtgga ggaggccgcc aagcaggcgg gcgcctcgca cgcctacctc
     1021 atggagcagc ccatggcggc ggccatcggc gcccgcctgc cggtgcacga gcccatgggc
     1081 aacatggtcc tggacgtggg cggcggcacc agcgaggtgg cggtgatctc gctgggcggc
     1141 gtggtggtgg ccaaggcggt gcgcgtcggc ggcttcgaca tcgacgccgc gatccaggcg
     1201 ttcatccgca accattacgc ggtggccatc ggcgagcgca cctccgagga gctcaagatg
     1261 gcgatcggct ccgcttaccc ccagtcggag gagcccaagg cggagatccg cgggcgcgag
     1321 ctggccaccg gcctgcccaa gacggtggtg atcggctccg acgagatccg cgaggccatc
     1381 gacgagcagg tcacccagat cgtcggtgcg gtcaccgaca cgctggcgga ctgcccgccg
     1441 gagctcaccc aggacgtgct gcgcagcggc atctggctgg tcggcggggg cgcgctgctg
     1501 cagggcctgg acgcgcgcat cgcgcaggag acgcaggtga aggtcaacgt cgtcgacgcg
     1561 ccgctggagg cggtcgtggt gggcgccggc accacgttgg agtccttcga caacctcaag
     1621 cacctgttcg tctagcgccc gtacactcgc cagcgacatg actgcgggcc tttccacgcc
     1681 gctccatctg gctgcctccc tgctggccct gtcggcggcg gtcggcctcg ccgtcgtcgg
     1741 gatcgtgggc ctgcgggcgc gccgccccgg ctccctgctg gtcaccgtgg gcgcgctcct
     1801 gctggccgtc ggtcacggcc tggcgggggc actggtgacg caggccgccg acgtcgccgc
     1861 gctgctggtc ccgctgccgc cgctgccccc gacgctgttc gccgccgcgg gcggggcgct
     1921 ggccgtgatc cggctgctgc cgcgcggcgg cgccgcggcg ctggccggcg tgggcctggg
     1981 cctgctgggg gccgccgagc tggtctcgcg ctcctcggcc accgcgggtg cggtgctgct
     2041 ggtgctgggc gcgctcggcg tcggcgtgtg gctgtgggcg ggcagcgccc ggcgcctgct
     2101 ggccaaggtc gccaccgcgt tcgtggcgct gctgctggcg ctgatcgtcc tggtcgcagg
     2161 catcctcagc ggccaggcct ccggccagct cgtggacgag gagctggccc gcctggagga
     2221 cctgtcggtg cggctggccg acgagatcgg ctcggcctgg ccgcaggacg ccatcgccgc
     2281 cgcccggccg ctgcgcaact ccggccagct ccttctgggc atcgcgccgg acgaccgcga
     2341 ccgcctggcg gacatcaacg acctgtcctt ccgccagcag gacttcttcg cgctgctcgg
     2401 cgccgacggc cgcgtggtga ccgcctactc cgacgacccg gcgctgctgt ccggctcctt
     2461 cacgctggcg ctcaccggcg cgcaggcggt ggaccgcatc ctggagggcc gcgacgagga
     2521 cggcgggctg gtcaccgtgg gcggcaagct cgtggccctg ggcgcggtga agctcgcggc
     2581 gcaggacgcg cgcgccgagg agccgcccat cggcgtgctt ttgaccggga gggtggtcga
     2641 cgaggtctgg gcggcgcaga ccgcccgcgc ccagggcgcc ggcatcgtgg tggaggtcgc
     2701 cgaggcgccc agcgtcgcct cgccggaact ccccgtcgcc gggaccgacc ccgcccgcga
     2761 cgtcatccgc gccaccacgc agggcgaagg caccggcgtg ctgaccggcg acgggcgcag
     2821 cgcctacgcc gcgtcggcgc ccatccgcga ccccaacacc gccacgctgg tgggccgcat
     2881 cgccacggtg cgcgccgccg aggtgatcgc cgaggtggag cgcgagcagg tcagctcgct
     2941 gttcgtcatc gccgtgctgg gcgccctggc cgccggcctg gtcggcgcgg cgatcacccg
     3001 gcgcctggtc gcgccggtgc gccggctcac cgaggccgcc gaagcggtgc gggcgggccg
     3061 cctggacacg cgcgcccaag tcgacacgcc cgacgagatc ggccggctgg gccgcacgtt
     3121 cgacgagatg accacgtccc tggcggcgca gtcggcggag ctggtcgagg ccgccgaggt
     3181 gcagtcccgg ctgcgcgcgc gcctggaggc gatgaccgac tcgatgggtg acgcgctggt
     3241 ggccgtcgac gccgccggcg agatcgtgac gttcaacccg gcggcggagc ggctcaccgg
     3301 gcgccgcgcc gccgacgtcg ccggcggccc cctggaccgg gcgctgcgcc tggccgcgac
     3361 gccggacacg ccggtcgcgg tggtcctcgg cgcgcccgac agcgaggacc aggtgcaggt
     3421 ccaggtcctg ctgcgcggcc agcgcgggcg gccggtgcag gccgcggtga cggcctcgcc
     3481 tgtgcgcgac gccgacggca aggtcctggg ccgcgtgctg gtgctgcgcg acatgacgcg
     3541 cgaggccgag gtcgagcgca tgaaggcgga gttcctggcc aacgtcagcc acgagctgcg
     3601 cacgccgctg acgcccatca agggctacgc cgacatcctg gcgcgccgcg acgtcgacaa
     3661 ggacaaggcc cgccgctacg cccagcagat cctggagtcc accgcgctgc tggagcgcat
     3721 cgtcgggatg atcgtggact tcgccgcgct ggactccggc caggtcgagc tggcgcgcga
     3781 gccggtgcgc ctgtccgacc tggtcgccga gacgctgggc gagtggcgcg cgcgccaccc
     3841 caagcgcgag ttcaagcggc gcgtcgccaa gacgctcccc gccgtgctgg cggacccggc
     3901 gatgctgcgc cgcgccctgg acgagctgct ggacaacgcc gtgaagttct ccccgcgcgg
     3961 cgagccggtc agcgtgacgg ccgcggtgga ggaggaccgc gccgggcgct gggtgcgcct
     4021 gtccgtgcgc gaccggggcg tgggcatcga cccggagacg gccgccaacg tcttcagcga
     4081 cttctatcag gtcgacgcca gcgagacccg ccagttcggc ggcttgggtc tgggcctggc
     4141 gctggtgcgc cgcatcgtcg agggcgtcgg cggccgggcc gccgtcgact ccgagccgga
     4201 cgagggcacg gaggttcacc tgctgctgcc ggctgccgaa gacacaggca ggaaaccgcg
     4261 cggcaccagg agttccggct gacgccgcat gggctgcgcg gcggaccccc ggacctacca
     4321 tgggacgcag catgaaggcg ggactcgcgg cgctgctctc gctggccatc gccctggcgc
     4381 tggccggttg ttccaccgcg cgcctgggtg gcgccgccgg gcggctgacc cagggcgaga
     4441 cggtgatggt gcagcagggc gacggctggc gcgccctggc ggccggtgac agcgtcccgg
     4501 acggcgcccg cgtgcgcacc ggcctggaga ccgcgcggct ggagctgcag cgcggcgaac
     4561 tgtggctggg ccactccgcc accgtgcgcc tgcgcggcga cgacgtggag gtcgagcgcg
     4621 gcgagatcgt cctgcacagc cagggcgccc gcgcctcctg gcaggccgtc gaggtcaccg
     4681 gcgagggtgt gttccgcctg gcgccgggcg cgtccccgcg cgtgggcgtg tacgcgggcg
     4741 aggtcacggt cgcgcgtccc ggcgagcagc ggcgcctgcc ggcgctgcgc cagcttggcc
     4801 tggcggcccg gcgcctgccc gcggagccgg acccgctgta ctacgacacc ggcgacgact
     4861 gggaccgcga gctgttgccc aaggcgatct cgttcgacga ggaggtgtcg cgcctggcgc
     4921 gcggcatcga ccgcgagtac ggcacggacc cgcagccggc ggacttctac accagcttca
     4981 cggccgtgcg cccggagacc gtgcccgtgc tggccgccac ggcgcgccgc gccgacgccg
     5041 acggcagctt cggcccgccg tcggacgcgc tggtgacgct gttcgtctcc tccgcggtcg
     5101 cggccgccca gggcctggcg atcgaggacg cggtgcgccg ggtcgccgac aagcgcgcgg
     5161 tcggcgcgcg ctgggggctc atcgccgtgg aggaggacat cgacgtgcag cgcctggccg
     5221 cggccgtcga cctcgcgcgg gagcgccgcc agatcgtggc gccggacgac gagcgcacca
     5281 tcgg
//
LOCUS       DATMKC010000304         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_338440, whole genome shotgun sequence.
ACCESSION   DATMKC010000304 DATMKC010000000
VERSION     DATMKC010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338440"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VNU01_09000"
     CDS             <1..468
                     /locus_tag="VNU01_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32792.1"
                     /translation="VPGRAPQGAARARGAQPARRPHLRVVGQPRHGMRYFVLAVTIGA
                     LGVFGIVGLHALAAEQSFAVRGLEREVRDLSLAYDELTAEIAALESPERVRQVALGQL
                     GMVPASRPGFLVADAPERGGSGDDDFVAARGTLPAPGGSDVADPVKQVLAEGS"
     gene            496..2418
                     /locus_tag="VNU01_09005"
     CDS             496..2418
                     /locus_tag="VNU01_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWH32793.1"
                     /translation="MSGQSTLTRARPRPAAPSGASRRGGPPPRWPPPSWGPAAAEWYG
                     RLSAALRRSRSRRRLTVMLAGYLVLTLLMSWRLVSIQVLQSEEYQSLAARQTHRDIEL
                     PARRGRLYARDGEPLAMSLEAATVYANPRVYERNGIDPSFIAATLAPLVDRPLLDVMR
                     ALTKDTSFSFVARQLPREVGEQIEALKLPGIQLLSEPRRVYPSDGLAAQTIGFAGVDN
                     QGLAGLEAQYESVLAGKPGRMWLERAPRGLTIDHGSREVEPSVAGTDLVLTLDREIQH
                     ATERALADAVAEHGAVGGSAVVVDAESGEVLAMASAPTYDPARIDGSDEYARRNRVVT
                     DVFEPGSVNKVITAAAALEAGLVRPDEVFTVPDTFPVAGKRFKDSSPHETKPMTFAQI
                     MEESSNVGTIQVALRLGEERMYRALRAFGYGERTGLGFPGESAGLLWPTEDWSGTSLP
                     TISIGQGVSATLLQVAQVFGVIANGGEWVEPLLVRGEVGPDGMLRQGETPKRRRVVSR
                     ETATQIAAMLSAVVEGERGTGTRAAVPGYRVAGKTGTAQKASTTRRGYEDGAYIASFA
                     GFAPADNPRLVAAVMLDEPDTIYGGVAAAPVFSEIMSFALGHRRIPPSDAAAAARAAE
                     PARAAAPPAAAGDDDG"
BASE COUNT          299 a          927 c          907 g          297 t
ORIGIN      
        1 gtcccgggcc gtgcccccca aggcgcagcc cgggcgcgcg gcgcgcagcc ggcccgccgt
       61 ccgcacctgc gcgtggtcgg ccagccccgc cacggcatgc gctacttcgt gctggcggtg
      121 acgatcggcg ccctgggcgt gttcggcatc gtcgggctgc acgcgctggc ggccgagcag
      181 tccttcgccg tgcgcggcct ggagcgggag gtcagggacc tgtcgctggc ctacgacgag
      241 ctcaccgccg agatcgcggc gctggagtcc ccggagcgcg tgcgccaggt cgccctgggc
      301 cagttgggca tggtgccggc gagccggccg gggttcctgg tggccgacgc ccctgagcgt
      361 ggcggatccg gtgacgacga tttcgtggcg gcgcgcggca cactgcccgc acccggcggc
      421 agcgacgtcg ccgaccccgt gaagcaggtg ctggccgagg gctcctaagc ccgctgaacg
      481 cacgtcagga gcggcatgtc cgggcaatcc acgctcaccc gagcgcggcc tcgccccgcg
      541 gcgccttccg gcgcctcccg ccgcggcggc ccgccgccgc ggtggccgcc cccgtcgtgg
      601 ggacctgccg cggccgagtg gtacggccgg ctcagcgccg cgctgcgccg ctcgcgcagc
      661 cgccgccgcc tgaccgtgat gctggccggc tacctggtgc tgaccctgct gatgtcgtgg
      721 cggctggtca gcatccaggt gctgcagtcc gaggagtacc agagcctcgc ggcgcggcag
      781 acccaccgcg acatcgagct gcccgcgcgc cgcggacgcc tgtacgcgcg cgacggcgag
      841 ccgctggcga tgtcgctgga ggccgccacc gtctacgcca acccgcgcgt gtacgagcgc
      901 aacggcatcg atcccagctt catcgccgcg acgctggcgc ccctggtcga ccggccgctg
      961 ctggacgtca tgcgcgcgct caccaaggac acgtcgttct ccttcgtcgc ccgccagctc
     1021 ccgcgcgagg tgggcgagca gatcgaggcg ctgaagctgc ccggcatcca gctgctcagc
     1081 gagccccgcc gcgtctaccc ctcggacggc ctggccgccc agaccatcgg cttcgccggc
     1141 gtcgacaacc aggggctggc cggcctggag gcccagtacg agtccgtcct ggcgggcaag
     1201 cccgggcgca tgtggctgga gcgcgccccg cgcgggctga ccatcgacca cggctcccgc
     1261 gaggtggagc catccgtggc cggcaccgac ctggtgctga cgctggaccg cgagatccag
     1321 cacgccaccg agcgcgcgct ggccgacgcc gtggccgagc acggcgcggt gggcggctcc
     1381 gcggtcgtgg tggacgccga gtccggcgag gtcctggcca tggcgtcggc accgacctac
     1441 gacccggcgc gcatcgacgg ctccgacgag tacgcgcggc gcaaccgcgt ggtcaccgac
     1501 gtgttcgagc cgggcagcgt gaacaaggtc atcaccgcgg ccgcggcgct ggaggccggc
     1561 ctggtgcgcc ccgacgaggt gttcaccgtc ccggacacct tccccgtggc cggcaagcgc
     1621 ttcaaggact cctcgccgca cgagaccaag ccgatgacgt tcgcgcagat catggaggag
     1681 tcctccaacg tgggcaccat ccaggtggcg ctgcgcctgg gggaggagcg catgtaccgc
     1741 gcgctgcgcg ccttcggcta cggcgagcgc accggcctgg gcttccccgg cgagtccgcg
     1801 ggcctgctgt ggccgaccga ggactggtcc ggcaccagcc tgcccacgat ctccatcggc
     1861 cagggcgtgt ccgccacgct gctgcaggtg gcgcaggtgt tcggcgtgat cgccaacggc
     1921 ggcgagtggg tcgagcccct gctggtgcgc ggcgaggtcg ggccggacgg gatgctgcgc
     1981 cagggcgaga cgcccaagcg gcggcgggtg gtgtcgcgtg agaccgcgac gcagatcgcc
     2041 gccatgctga gcgccgtggt cgagggcgag cggggcaccg gcacgcgcgc cgccgtgccc
     2101 ggctaccggg tggcgggcaa gacggggacc gcgcagaagg cctccaccac gcggcgcggg
     2161 tacgaggacg gcgcctacat cgcgagcttc gccggcttcg ccccggccga caacccgcgc
     2221 ctggtggccg ccgtgatgct ggacgagccc gacacgatct acggcggcgt ggccgccgcg
     2281 cccgtgttca gcgagatcat gagcttcgcc ctgggccacc gccgcatccc gcccagtgac
     2341 gcggccgccg cggcccgggc cgccgagccg gcgcgcgccg ccgccccgcc ggccgcggcc
     2401 ggcgacgacg acggctgacc gcgtgccgac
//
LOCUS       DATMKC010000305         7828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_49365, whole genome shotgun sequence.
ACCESSION   DATMKC010000305 DATMKC010000000
VERSION     DATMKC010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49365"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="cgtA"
                     /locus_tag="VNU01_09010"
     CDS             <1..375
                     /gene="cgtA"
                     /locus_tag="VNU01_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HWH32794.1"
                     /translation="VLRPADGKGQGFEVEAAEDGWRVRGERVERWVVMADLDNEEAVR
                     YLQGRLVRAGVEKALLDAGARRGDAVEIAGAVFDFEPERADMPDDEDDDDDALEPLVD
                     EVMFEDEDDDFALAEQQEDDAE"
     gene            372..1547
                     /gene="proB"
                     /locus_tag="VNU01_09015"
     CDS             372..1547
                     /gene="proB"
                     /locus_tag="VNU01_09015"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWH32795.1"
                     /translation="MTRPQAATEPRFPRPSRAVVKVGSSSLRGDGGRGIDRALVSDLA
                     RQIAAVRAAGTEVVLVTSGAVAAGMTGLGMAQRPSDTPSLQAAASVGQGVLMHTYSEA
                     FASYDVVCGQVLLTPDDVVHRERYLNARHTFTRLLELGALPIVNENDTVATDELRFGD
                     NDRLAALVASMLGAQLLVLLSDVEGLLDGDPAIDPAAPLLDRVDDLAALDARHFGGTG
                     SELGSGGMASKLEAARVARFSAAHTVIANARRDGVVTDAVAGAGVGTWFVPARRRPDS
                     RKLWIAFAKRAAGHLVVDSGAVRALTAGKKSLLAAGVVDAAGDFAAGDAVQVRSPDGV
                     VIARGLVGYSREEVLRIRGRSSEELARELGEAYTREVIHRDALVMLSEGLPAPADQG"
     gene            1556..2902
                     /locus_tag="VNU01_09020"
     CDS             1556..2902
                     /locus_tag="VNU01_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH32796.1"
                     /translation="MRQRVRVHPVVVDEVLVQLVGRQEPQLALRALAHALTLALLALA
                     TLGAAAPAGAQARDPLRDQLWHLDRVHAEDAWPVSRGAGMVLAVVDTGVDASHPDLAG
                     NVGRGIDLVEEGTPPDDPNGHGTLVAGVIAAVQGNGLGVAGASPDVRIMPVRVLDKDG
                     NGNSELVARGVRWAADNGAHVINLSLAEVPGLLSSLGGLFGDELSDAIRYADERGAVV
                     VAAAGNDGRSSTPYPRDLPLLVVGATDRGDQVWSSSNRDARTVFAPGVEIVSTWRDHG
                     YGRANGTSFATPIVSALAAILRQRGLTASEARAQIEATATRVGTGRGRVDFARAVGVE
                     PAQSPSPAATAGPPGGQPTASPGATLEATPPPRDSQPPGADGQAQPPPPRTAPPEVVS
                     PLPEGAGDPVAGPDVDPDVQVRPGLEVRQSREPLRWLGLVALLGVVAAFARAARRR"
     gene            2984..4228
                     /locus_tag="VNU01_09025"
     CDS             2984..4228
                     /locus_tag="VNU01_09025"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWH32797.1"
                     /translation="MSSVRHACEAAKAAAPALARASTARKNAALEAMAAALDANHARV
                     LEANAEDVARGRAEGLSEALIDRLTLTGARLDAIAQALRDLIALKDPIGELVRGWTLP
                     NGLEVRQVRVPLGVVAMVYEGRPNVTVDAAGLALKSGNACVLRGSSSAISSNAALAGL
                     LRGALEAQGLPADGVVLIEDTSRDSVKELGRMRGLVDLLIPRGGAGLIQAVVQDSVVP
                     VIETGVGNCHVYVDAAADLDKARAIAFNAKVHRPSVCNAAETLLVHRAVAEEFLPGMA
                     KELTAAGVELVGDADAQRLAGAAPATDSDWDTEYLDLKLAVRVVDGLDEALAHIARHG
                     TLHTEAIVTEDRAAARRFVQEVDAAAVMVNASTRFTDGGEFGFGAEIGISTQKMHARG
                     PMGTQELTTTKYVVEGEGQVRS"
     gene            4225..5106
                     /locus_tag="VNU01_09030"
     CDS             4225..5106
                     /locus_tag="VNU01_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWH32798.1"
                     /translation="MTDPRFAGLEDTSSRLESAGYLPDRAITTVTFLADRLGKPVLVE
                     GPAGVGKTELAKAIASARGAQLVRLQCYEGLDEAKALYEWNYKKQLLRIQADSDRAWE
                     DTKDDIFGPEYLLTRPLLQAITDDIETVLLIDEVDKVDFEFEALLLEILSDFQVTVPE
                     MGTVAAVRRPFVVLTSNNTRELSEALKRRCLYLHMDYPDIEREKAIVRSRVPEVPEQL
                     ADQLVRIVRSLRNLELRKAPSISETLDWARTLLELGYSGVDEGVAQTTLSVLLKHQSD
                     IDKALAHLEMAADPGSN"
     gene            5110..6546
                     /locus_tag="VNU01_09035"
     CDS             5110..6546
                     /locus_tag="VNU01_09035"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWH32799.1"
                     /translation="MAAPADTRLSGLQRRVLDLAHALREANVPVAVSDGLDAMRASAL
                     IDLLDRGQLREALAATMVKSPSHRATFDLLFDLYFPARGTQPLPGEAGDEAERPAEYD
                     GDTHHQEARDIDQFLAELVMRVMEGDDAAIRRLAMEAVESFGAVRQRDGRVSFFAYRV
                     FRNFNLGGLLRRLMTESGMDGDAEDAASALTRRLQRDEFEQRLRRFREEIESEIRRRQ
                     VEQQGADEIAKRMARPLPEDLDFFRISAEESESMRRQIRPLARKLATRVAVKRRRARD
                     GRLDVRRTIRHSLSTGGVPADPAFKARKHHRPELVLICDVSGSVAAFARFTLMLTHAL
                     QGQFSKVRSFAFIDTVDEVTRLFSGGDFSDAIKRLHTDANVVWLDGHSDYGHAFTAFH
                     ARYADAITPKTTLLILGDGRNNYRASNAWVLKDLKRRARRVYWLNPEPIQHWGTGDSI
                     ADEYARFTDGMIECRNLRQLAEFVERVV"
     gene            6556..7158
                     /gene="nadD"
                     /locus_tag="VNU01_09040"
     CDS             6556..7158
                     /gene="nadD"
                     /locus_tag="VNU01_09040"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWH32800.1"
                     /translation="MRRIGLMGGSFDPIHLGHLVTAEQARVDLGLDEVVFIPAGQPWQ
                     KEHTTPAEHRYLMTVLATSANPYFSVSRVEIEREGPTYTVDTLRTLRARHADADLWFI
                     SGADAIVNIVTWRDAAEALDLATFVAATRPGHDVAALQEPGIRERIVTLDVPALAISS
                     TDIRARVAADRSVRYLIPREVEEHIRKHGLYGATAQVGGW"
     gene            7155..>7828
                     /locus_tag="VNU01_09045"
     CDS             7155..>7828
                     /locus_tag="VNU01_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWH32801.1"
                     /translation="MTGRVARLEARRRRRRRLLTTAAAVTVAAAAVAALAGGALRQGD
                     DVTETPSGDATPAASARARTMLLVRAPHEPGPAKGVTLFAVGPADGQAAVVLLPVGTL
                     MDVPGHGLERLGAAFQYGGAALVEATVENTLGVDVDHSAAVSDAGLAALLGRVGPVEV
                     DVDRRLVARPGDGEPSLRFAAGPQHLDGPRLVEFLAFRDQPDDELSAFPRQERVWRAL
                     FGALAGP"
BASE COUNT         1038 a         2823 c         2988 g          979 t
ORIGIN      
        1 gtgctgcgtc ccgccgacgg taagggccag ggcttcgagg tggaggcggc cgaggacggc
       61 tggcgcgtgc gcggggagcg cgtcgagcgc tgggtcgtca tggcggacct ggacaacgag
      121 gaggccgtgc gctacctgca aggccgcctg gtgcgcgccg gcgtggagaa ggcgctgctg
      181 gacgcgggcg cgcggcgcgg cgacgccgtg gagatcgccg gcgctgtgtt cgacttcgag
      241 cccgagcgcg ccgacatgcc ggatgatgag gacgacgacg acgatgcgct ggagccgctc
      301 gtcgacgagg tcatgttcga ggacgaagac gacgacttcg cgctcgccga gcagcaggag
      361 gacgacgccg agtgacccgg ccgcaggccg cgaccgagcc gcgcttcccc cggcccagca
      421 gggccgtggt gaaggtcggc tcctccagcc tgcgcggcga cggcggccgc ggcatcgacc
      481 gcgccctcgt gagcgacctg gcgcggcaga tcgccgccgt gcgcgccgcc ggcacggagg
      541 tcgtcctggt caccagcggc gcggtcgccg ccggcatgac cggcctcggc atggcgcagc
      601 ggccgtcgga cacgcccagc ctccaggcgg cggccagtgt cgggcagggc gtgctgatgc
      661 acacctacag cgaggcgttc gcctcctacg acgtggtctg cggccaggtc ctgctcaccc
      721 cggacgacgt ggttcaccgc gagcgctacc tcaacgcccg gcacaccttc acgcgcctgc
      781 tggagctggg cgcgctgccg atcgtgaacg agaacgacac ggtggccacc gacgagctgc
      841 gcttcgggga caacgaccgc ctcgcggcgc tggtcgcgag catgctcggc gcccagctcc
      901 tggtgctgct gtccgacgtc gaggggctgc tggacgggga cccggccatc gacccggccg
      961 ccccgctgct ggaccgcgtc gacgacctgg ccgcgctcga cgcccggcac ttcggcggca
     1021 ccggctcgga gctgggcagc gggggcatgg cctccaagct ggaggccgcg cgcgtcgcgc
     1081 gcttcagcgc cgcgcacacc gtgatcgcca acgcccgccg cgacggcgtg gtgaccgacg
     1141 ccgtcgccgg ggcgggggtc ggcacgtggt tcgtcccggc gcgccgccgc cccgacagcc
     1201 gcaagctgtg gatcgccttc gccaagcgcg ccgccggtca tctggtggtc gacagcggcg
     1261 ccgtgcgcgc gctcacggcc ggcaagaagt cgctgctcgc cgccggcgtg gtggacgcgg
     1321 ccggggactt cgccgccggt gacgccgtgc aggtgcgcag cccggacggc gtcgtcatcg
     1381 cgcgcggcct ggtgggctac tcgcgcgagg aggtcctgcg catccgcggg cgctccagcg
     1441 aggagttggc gcgcgagctc ggcgaggcgt acacgcgcga ggtgatccac cgcgacgcgc
     1501 tggtgatgct gtccgaaggg ctcccagcgc ccgccgacca gggctagggg cggcgttgcg
     1561 gcagcgcgtc cgggtccacc ccgtggtcgt cgacgaggtg ctggtccagc tcgtcggccg
     1621 tcaggaaccg cagctcgcac ttcgggcact ggcgcatgcg ctcaccctag cgctgctcgc
     1681 gctggcgacg ctcggcgcgg ccgctccggc cggcgcccag gcgcgcgacc cgctgcgcga
     1741 ccagctctgg cacctggacc gcgtgcacgc cgaggacgca tggccggtca gccgcggcgc
     1801 cggcatggtc ttggccgtgg tcgacaccgg ggtggacgcg tcccacccgg acctcgcggg
     1861 caacgtcggg cgcggcatcg acctggtgga ggagggcacg ccccccgacg accccaacgg
     1921 ccacggcacg ctggtggccg gcgtgatcgc ggcggtgcag ggcaacggcc tgggtgtggc
     1981 cggggcgtcc cccgacgtgc gcatcatgcc ggtgcgcgtg ctggacaagg acggcaacgg
     2041 caactcggag ctggtcgcgc gcggcgtgcg ctgggccgcg gacaacggcg cgcacgtgat
     2101 caacctgagc ctcgccgagg tcccgggact gctgtccagc ctgggcgggc tgttcggcga
     2161 cgagctgagt gacgccattc gctacgccga cgagcggggc gcggtcgtgg tcgccgcagc
     2221 gggcaacgac ggccgctcca gcacgccgta cccgcgcgac ctgccgctgc tggtggtggg
     2281 tgccacggac cgcggcgacc aggtgtggtc gtcgtccaac cgcgacgcgc gcacggtgtt
     2341 cgcgccgggc gtggagatcg tgtcgacctg gcgcgaccac ggttacggcc gggccaacgg
     2401 caccagcttc gccacgccga tcgtctcggc gctggccgcg atcctgcgcc agcgcgggct
     2461 gaccgcctcg gaggcgcgcg cccagatcga ggccaccgcc acacgcgtcg gcaccggccg
     2521 cggccgcgtg gacttcgcgc gggcggtggg cgtggagccg gcccagagcc cgtcgcccgc
     2581 cgccacggcc ggtccgcccg gcgggcagcc gacggcgtcg cccggcgcca ccctggaggc
     2641 gaccccgccg ccgcgcgatt cgcagccgcc cggcgcggac gggcaggcgc agccgccgcc
     2701 gccgcgcacg gcgcctcccg aggtggtgtc gcccttgccc gagggcgcgg gcgatcccgt
     2761 tgccgggccg gacgtcgacc cggacgtcca ggtgcggccg ggcctggagg tgcgccagtc
     2821 ccgcgagccg ttgcgctggc tcgggctggt cgcgctgctc ggcgtggtcg cggcgttcgc
     2881 ccgcgccgcg cgccggcgct gagcggccgc ccgcttggcg gttgcgtgac gcaacgccgt
     2941 accatggcgg gactgcccgt gtccgcggag aggagggcgg tgcatgtcca gcgttcgtca
     3001 cgcctgtgag gcggccaagg ccgccgcgcc cgcgctcgcg cgggcctcca cggctcgcaa
     3061 gaacgccgcg ctcgaagcca tggccgcggc gctcgacgcg aaccacgcgc gcgtgctgga
     3121 agccaacgcc gaggacgtcg cccgcggccg cgccgagggg ctgtccgagg cgctgatcga
     3181 ccggctcacg ctgaccgggg cgcgcctgga cgccatcgcg caggcgctgc gcgacctgat
     3241 cgcgctcaag gaccccatcg gtgagctcgt ccgcggctgg acgctgccca acggcctgga
     3301 ggtgcgccag gtgcgggtgc cgctgggcgt ggtggcgatg gtctacgagg gccggcccaa
     3361 cgtgacggtg gacgccgccg gcctggcgct caagagcggc aacgcctgcg tgctgcgcgg
     3421 ctcgtccagc gcgatcagtt ccaatgcggc gctggccggg ctgctgcgcg gcgcgctgga
     3481 ggcccagggc ctgccggcgg acggcgtcgt gctgatcgag gacacctcgc gcgactccgt
     3541 caaggagctc ggccgcatgc gcgggctggt ggaccttttg atcccgcgcg gcggcgccgg
     3601 gctcatccag gccgtggtgc aggactcggt tgtgcccgtc atcgagaccg gcgtgggcaa
     3661 ctgccacgtg tacgtggacg ccgcggccga cctggacaag gcgcgggcca tcgcgttcaa
     3721 cgccaaggtg caccgcccca gcgtgtgcaa cgccgcggag acgctgctgg tccaccgcgc
     3781 cgtggcggag gagttcctgc ccggcatggc caaggagctc accgccgcgg gggtggagct
     3841 ggtgggcgac gccgacgcgc agcgcctggc gggcgcggcg cccgccaccg acagcgactg
     3901 ggacaccgag tacctggacc tcaagctggc ggtgcgggtg gtggacggcc tggacgaggc
     3961 gctggcgcac atcgcccgcc acggcacgct gcacaccgag gccatcgtca cggaggaccg
     4021 cgccgcggcg cgccgcttcg tccaggaggt ggacgccgca gccgtgatgg tgaacgcgtc
     4081 cacgcgcttc accgacgggg gcgagttcgg cttcggcgcg gagatcggga tctccacgca
     4141 gaagatgcac gcgcgcgggc cgatgggcac gcaagagctc accacgacga agtacgtggt
     4201 ggagggagag ggccaggtgc gctcgtgacg gacccccgat tcgccggctt ggaggacacg
     4261 tcctcccgat tggagtccgc cgggtacctg ccggaccgcg cgatcaccac ggtgacgttc
     4321 ctggccgacc gcctgggcaa gccggtgctg gtcgagggcc ccgccggcgt cggcaagacc
     4381 gagctggcca aggccatcgc cagcgcgcgc ggcgcgcagc tggtgcgcct gcagtgctac
     4441 gagggcctgg acgaggccaa ggcgctgtac gagtggaact acaagaagca gctgttgcgc
     4501 atccaggcgg actccgaccg cgcctgggag gacaccaagg acgacatctt cggcccggag
     4561 tacctgctga cgcggccgct tttgcaggcg atcaccgacg acatcgagac ggtgctgctg
     4621 atcgacgagg tcgacaaggt cgacttcgag ttcgaggcgc tgctgctgga gatcctcagc
     4681 gacttccagg tcaccgtccc ggagatgggc accgtggcgg cggtgcgccg gccgttcgtg
     4741 gtgctcacgt ccaacaacac gcgggagctg agcgaggcgc tgaagcggcg ctgcctgtac
     4801 ctgcacatgg actacccgga catcgagcgc gagaaggcga tcgtgcgctc gcgcgtgccg
     4861 gaggtgcccg agcagctcgc cgaccagctc gtgcgcatcg tgcgcagcct gcgcaacctg
     4921 gagctgcgca aggcgccgtc gatctccgag acgctggact gggcgcgcac gctgctggaa
     4981 ctgggctaca gcggcgtcga cgagggcgtg gcgcagacca cgctcagcgt gctgctcaag
     5041 caccagagcg acatcgacaa ggcgctcgcg cacctggaga tggccgccga cccggggagc
     5101 aactagccgg tggccgcccc ggccgacacg cggctgtccg ggctgcagcg ccgggtgctc
     5161 gacctcgccc acgcgctgcg ggaggccaac gtgccggtgg cggtcagcga cggcctggac
     5221 gcgatgcggg cgtccgcgct gatcgacctg ctggaccgcg ggcagctccg cgaggcgctg
     5281 gccgccacga tggtgaagtc gcccagccac cgcgccacgt tcgacctgct gttcgacctg
     5341 tacttcccgg cgcgcgggac gcagcccctg cccggcgagg ccggcgacga ggccgagcgg
     5401 ccggccgagt acgacggcga cacgcaccac caggaggcgc gcgacatcga ccagttcctg
     5461 gcggagctgg tcatgcgcgt catggagggc gacgatgccg cgatccggcg gctggcgatg
     5521 gaggcggtgg agagcttcgg cgcggtgcgc cagcgcgacg ggcgcgtgag cttcttcgcc
     5581 taccgggtgt tccgcaactt caacctgggc gggctgctgc gccgcctgat gaccgagtcc
     5641 ggcatggacg gcgacgcgga ggacgcggcg agcgccctga cccggcggct ccagcgcgac
     5701 gagttcgagc agcgcctgcg gcgcttccgc gaggagatcg agtcggagat ccgccgccgc
     5761 caggtagagc agcagggcgc ggacgagatc gccaagcgca tggcccgccc gctgccggag
     5821 gacctggact tcttccgcat cagcgcggag gagtccgagt cgatgcggcg ccagatccgc
     5881 cccctggcgc gcaagctggc cacgcgcgtg gcggtcaagc gacggcgcgc ccgcgacggc
     5941 cgcctggacg tgcggcgcac catccgccac tcgctgtcca ccggcggcgt gcccgccgac
     6001 cccgcgttca aggcgcgcaa gcaccaccgc ccggagctgg tgctgatctg cgacgtgtcc
     6061 ggctccgtgg ccgcgttcgc gcgcttcacg ctgatgctga cgcacgcgct gcagggccag
     6121 ttcagcaagg tgcgcagctt cgcgttcatc gacaccgtgg acgaggtcac ccggctgttc
     6181 tccggcggcg acttcagcga cgcgatcaag cgcctgcaca cggacgccaa cgtggtgtgg
     6241 ctggacggcc acagcgacta cggccacgcg ttcacggcgt tccacgcgcg ctacgccgac
     6301 gcgatcacgc ccaagaccac gctgttgatc ctgggcgacg ggcgcaacaa ctaccgggcg
     6361 tccaacgcgt gggtgctcaa ggacctcaag cgccgcgcgc gccgcgtgta ctggctgaac
     6421 ccggagccga tccagcactg gggcacgggg gactccatcg ccgacgagta cgcccgtttc
     6481 accgacggca tgatcgagtg ccgcaacctg cgccagctcg ccgagttcgt cgagcgggtg
     6541 gtgtagacac tcgtcatgcg gcgcatcggc ctcatgggcg gcagcttcga ccccatccac
     6601 ctcggccacc tggtcacggc cgagcaggcg cgtgtggacc tggggctgga cgaggtggtg
     6661 ttcatcccgg ccggccagcc gtggcagaag gagcacacca cgccggccga gcaccgctac
     6721 ctcatgaccg tgctggccac gtccgccaac ccgtatttct cggtcagccg cgtggagatc
     6781 gagcgggagg ggcccaccta cacggtggac accctgcgca cgctgcgggc gcgccacgcc
     6841 gacgcggacc tgtggttcat ctccggcgcg gacgccatcg tcaacatcgt gacgtggcgc
     6901 gacgcggcgg aggcgctgga cctcgcgacg ttcgtggccg ccacgcggcc gggccacgac
     6961 gtcgccgcgc tgcaggagcc gggcatccgc gagcgcatcg tgacgctgga cgtgccggcg
     7021 ctggcgatct cgtccacgga catccgcgcg cgcgtggccg cggaccgctc cgtgcgctac
     7081 ctgatcccgc gcgaggtgga ggagcacatc cgcaagcacg gcctgtacgg ggcgacggcc
     7141 caggtcggtg gctggtgacg gggcgggtcg cgcgcctgga ggcgcgccgg cgccggcgcc
     7201 ggcgcctgct caccacggcc gcggcggtga ccgtcgcggc ggccgcggtg gccgcgctgg
     7261 ccggtggcgc gctgcgccag ggcgacgacg tcacggagac gccgtccggg gatgcgacgc
     7321 ctgccgcttc cgcgcgcgcc cgcacgatgc tgctggtgcg cgctccccac gagcccggtc
     7381 cggccaaggg cgtgacgctg ttcgcggtgg gcccggcgga cggccaggcg gcggtggtgc
     7441 tgctgccggt gggcacgctg atggacgtgc ccggccacgg cctggagcgc ctgggcgcgg
     7501 ccttccagta cggcggggcc gcgctggtcg aggccaccgt ggagaacacc ctgggcgtgg
     7561 acgtcgacca cagcgccgcg gtgagcgacg cgggcctggc cgcgctgctc ggccgcgtcg
     7621 gcccggtcga ggtcgacgtg gaccgccggc tggtggcccg gccgggggac ggcgagccgt
     7681 cgctgcgctt cgccgccggc ccccagcacc tggacggccc gcgcctggtc gagttcctgg
     7741 cgttccgcga ccagccggac gacgagctca gcgcgttccc gcgccaggag cgcgtgtggc
     7801 gggcgctgtt cggcgcgctg gccggccc
//
LOCUS       DATMKC010000306         3832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_74597, whole genome shotgun sequence.
ACCESSION   DATMKC010000306 DATMKC010000000
VERSION     DATMKC010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74597"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /gene="folP"
                     /locus_tag="VNU01_09050"
     CDS             <1..502
                     /gene="folP"
                     /locus_tag="VNU01_09050"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWH32802.1"
                     /translation="VNDVSGAADRKLLEVAAAHKAVYVLMHTRGTPADMQSLADYDDV
                     VAEVYEFLADGLARCTAAGLPLERVVVDPGIGFAKTPEHNLRLLAALRQFRGLGRPVL
                     VGASRKSFLGAILGGAPPAERLEGSLVCAAHAAAAGAAVVRVHDVAPTVRAVRTMAAI
                     AAEESP"
     gene            499..927
                     /locus_tag="VNU01_09055"
     CDS             499..927
                     /locus_tag="VNU01_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWH32803.1"
                     /translation="MSELGGTGEAAPASELEAANTAFYDAFEAMSLDRMEGVWATDAD
                     AYCVHPGGEILRGWPRVRRAWAAVFASTSYIQFIVTNVEAAEHGDVGVVSCVENVLAG
                     EGADHLHGGTAVATNVFVRRDGRWRMLAHHASPMLRVEGA"
     gene            924..1964
                     /locus_tag="VNU01_09060"
     CDS             924..1964
                     /locus_tag="VNU01_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWH32804.1"
                     /translation="MTALMRAVRFHDHGGPEVLRLEALAVPAAGPGEVRVKVAACGLN
                     HLDIWTRRGMERVDVPLPHVPGVDIAGHVDSVGPGVEGWAEGDAVVVYPLETCGSCPA
                     CSAGQEQRCRHGRMIGGYHRDGGYADYVTVRAATLVPWPEGLSAVEAAALPVAYTNAW
                     HMVHDKGEATHGDDVLVTAASGGVATACVQLASAAGCRVIATVSSPDKADPVAGLGAH
                     DVLVGRDLDHGEVRALTGGKGVEIALDHVGGDMVMSCVRSLAAGGRLVTTGASAGPKP
                     ELDLRYLFMRDLDVRGAYMGPKSALERVMRLAGQGELRPLIHEVFPLERAADAQRVLE
                     ERKVIGKVVLEL"
     gene            1961..2353
                     /locus_tag="VNU01_09065"
     CDS             1961..2353
                     /locus_tag="VNU01_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32805.1"
                     /translation="MSHVVDPDGLPYAPEKLERKPVVGAEGFFALDLRAGRVVEVEEF
                     PEARKPAWKLAVDFGPVVGLLRTSAQVTNYAREELVGRMVVGAINLGEKRIAGFTSQF
                     LVLGTLRPDGTVDLLDVEPRTAPGAPVA"
     gene            complement(2355..3071)
                     /locus_tag="VNU01_09070"
     CDS             complement(2355..3071)
                     /locus_tag="VNU01_09070"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HWH32806.1"
                     /translation="MATGLSRRPVKAPSAPGVTSVPLLEGVAGFRDLGGFPTVDGAST
                     RKGVLFRSPCPSELTAADRRVLAALRVALRIDLRATFEVRDAPPVDLAELACAHVPVL
                     DAPDAARYARRLRSALHDRRDPAAVLAAFLDSEGEAFAKVFELLSERTPAVVHCTTGR
                     DRTGLVAGLALRLAGVPDNFIAVDYAESLPQGIEGDAGPILQALEKVDREFGSVRAYL
                     AEHGCPDAAIDRFRAAFRTG"
     gene            3202..3567
                     /gene="folB"
                     /locus_tag="VNU01_09075"
     CDS             3202..3567
                     /gene="folB"
                     /locus_tag="VNU01_09075"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWH32807.1"
                     /translation="MDRIEITGLRVFGRHGVFDWEQEQGQDFTVDVALDVDLRAAGAS
                     DELADTVDYGLLAERIAREVEDTQFQLLEALAQHLSDVVLSDERVRAVDIRIGKPDVA
                     LSVELHDVAVHVRRSREAG"
     gene            3573..>3832
                     /gene="folK"
                     /locus_tag="VNU01_09080"
     CDS             3573..>3832
                     /gene="folK"
                     /locus_tag="VNU01_09080"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWH32808.1"
                     /translation="MSVLAAWLPPRGGAFAFLGLGSNLGERMEHLQRAVDLLDDDART
                     RVEAVSSVYETDPVGGPEQGAYLNVALRVATRRSPRGLLRLC"
BASE COUNT          483 a         1330 c         1529 g          490 t
ORIGIN      
        1 cgtcaacgac gtctcgggcg ccgcggaccg caagctgctg gaggtggccg cggcgcacaa
       61 ggccgtgtac gtgctgatgc acaccagggg cacgcccgcc gacatgcagt cgctggcgga
      121 ctacgacgac gtggtcgccg aggtgtacga gttcctggcc gacgggctgg cgcgctgcac
      181 ggcggcgggg ctgccgctgg agcgcgtggt ggtggacccg ggcatcggct tcgccaagac
      241 gcccgagcac aacctgcgcc tgctggccgc cctgcggcag ttccgcggtc tggggcggcc
      301 ggtgctggtg ggcgcgtccc gcaagagctt cctgggggcg atcctgggtg gggcgccgcc
      361 ggcggagcgc ctggagggca gcctcgtgtg cgccgcgcac gccgcggccg ccggcgcggc
      421 ggtggtgcgc gtgcacgacg tggcccccac cgtgcgcgcc gtgcggacga tggccgcgat
      481 cgccgccgag gagtccccat gagcgagctg ggcgggacgg gcgaggcggc accggcctcg
      541 gagctggagg cggccaacac cgcgttctac gacgcgttcg aggccatgag cctcgatcgc
      601 atggaggggg tctgggccac ggacgccgac gcctactgcg tccaccccgg cggggagatc
      661 ctgcgcggct ggccccgggt gcgccgcgcc tgggcggcgg tgttcgccag caccagctac
      721 atccagttca tcgtgaccaa cgtcgaggcg gccgagcacg gcgacgtcgg cgtggtgagc
      781 tgcgtggaga acgtgctcgc gggcgagggc gcggaccatc tgcacggcgg caccgccgtg
      841 gccaccaacg tattcgtgcg ccgcgacggg cgctggcgca tgctcgccca ccacgcctcg
      901 ccgatgctgc gcgtggaggg ggcatgacgg cgctgatgcg ggccgtgcgc ttccacgacc
      961 acggcggtcc cgaggtgctg cggctggagg cgctggccgt cccggcggcc gggcccggcg
     1021 aggtgcgcgt caaggtggcc gcctgcgggc tgaaccacct ggacatctgg acccgccgcg
     1081 gcatggagcg cgtcgacgtg ccgctgccgc acgtgccggg cgtcgacatc gccggccacg
     1141 tcgacagcgt gggccccggt gtggagggct gggcggaggg cgacgccgtg gtggtgtacc
     1201 cgctggagac gtgcggctcc tgcccggcgt gctcggcggg gcaggagcag cgctgccgcc
     1261 acgggcgcat gatcggcggc taccaccgcg acggcggcta cgcggactac gtgacggtgc
     1321 gcgcggcgac gctggtgccc tggccggagg gcctgtccgc cgtggaggcg gccgcgctgc
     1381 ccgtggcgta cacgaacgcc tggcacatgg tccacgacaa gggcgaggcg acccacggcg
     1441 acgacgtgct ggtcaccgcc gcaagcggcg gcgtcgccac cgcctgcgtg cagctcgcct
     1501 ccgcggcggg ctgccgggtg atcgccacgg tgtccagccc ggacaaggcc gacccggtcg
     1561 ccgggctcgg tgcgcacgac gtcctggtgg gccgcgacct ggaccacggc gaggtgcgcg
     1621 cgctcaccgg cggcaagggc gtggagatcg cgctggacca tgtcggcggc gacatggtca
     1681 tgagctgcgt gcgctccctg gccgcgggcg ggcgcctggt gaccacgggc gcgagcgcgg
     1741 gccccaagcc ggagctggac ctgcgctacc tgttcatgcg cgacctggac gtgcgcgggg
     1801 cctacatggg gccgaagtcg gcgctggagc gcgtcatgcg cctggccggc cagggcgagc
     1861 tgcggccgct gatccacgag gtgttcccgc tggagcgcgc ggcggacgcg cagcgggtgc
     1921 tggaggagcg caaggtgatc ggcaaggtgg tgctggagct gtgagtcacg tggtggatcc
     1981 ggacgggctg ccgtacgcgc cggagaagct ggagcgcaag cccgtggtgg gggcggaggg
     2041 cttcttcgcg ctggacctgc gcgccggccg cgtggtggag gtcgaggagt tcccggaggc
     2101 gcgcaagccg gcctggaagc tggccgtgga cttcgggccg gtggtgggcc tgctgcggac
     2161 gtcggcgcag gtcaccaact acgcgcgcga ggagctcgtg ggccgcatgg tggtcggcgc
     2221 catcaacctt ggtgagaagc gcatcgcggg gttcacgtcg cagttcctgg tactggggac
     2281 cctgcggccg gacggcaccg tcgacctgct ggacgtggag ccccggaccg ctcccggcgc
     2341 cccggtcgcc tgactcagcc ggtgcggaag gcggcgcgga agcggtcgat cgcggcgtcg
     2401 ggacagccgt gctcggccag gtacgcgcgc acgctgccga actcgcggtc gaccttctcc
     2461 agggcctgca ggatcggtcc ggcgtcgccc tcgatgccct gcggcagcga ctcggcgtag
     2521 tccacggcga tgaagttgtc gggcacgccc gccaggcgca gcgccaggcc ggccaccagc
     2581 ccggtccggt cgcgtcccgt ggtgcagtgc accaccgcgg gggtgcgctc gctcagcagc
     2641 tcgaacacct ttgcgaacgc ctcgccctcg gagtccagga acgcggccag cacggccgcg
     2701 gggtcacggc ggtcgtgcag ggcgctgcgc aggcgccgcg cgtagcgggc ggcgtccggg
     2761 gcgtcgagca ccgggacgtg cgcgcaggcc agctccgcga ggtccaccgg cggcgcgtcg
     2821 cgcacctcga acgtcgcccg caggtcgatg cgcagcgcga cgcgcaacgc tgccaggacg
     2881 cggcggtccg cggcggtcag ctcggagggg cacggcgagc ggaacagcac gcccttgcgc
     2941 gtggacgcgc cgtcgacggt cgggaacccg cccaggtcgc ggaagccggc cacgccctcc
     3001 agcaggggca cggaggtgac cccaggcgcg ctcggcgcct tgacgggtcg gcgcgacagg
     3061 cccgtggcca tgggggaagc tgctccttcg caagctggcg gactgcgaac cgtaccccgc
     3121 ccggcgggcc tctatgctcg cgaaagccgt cgacgatcgg ctttgacgcc agtcggatcg
     3181 ccagggcccc tggaggagcg cgtggacagg atcgagatca ccgggctgcg ggtgttcggc
     3241 cgccacggcg tgttcgactg ggagcaggag cagggccagg acttcaccgt cgacgtggcg
     3301 ctggacgtcg acctgcgcgc cgccggcgcc tccgacgagc tcgcagacac ggtggactac
     3361 ggcctgctgg ccgagcgcat cgcccgcgag gtcgaggaca cccagttcca gctgctggag
     3421 gcgctggccc agcacctgtc cgacgtcgtc ctgtccgacg agcgcgtgcg cgcggtggac
     3481 atccgcatcg gcaagcccga cgtggcgctg tcggtggagc tgcacgacgt cgccgtgcac
     3541 gtccggcggt cccgcgaggc gggctgagcg tcgtgtcggt tctggccgca tggttgccgc
     3601 cccgcggggg cgcgttcgcg ttcctgggcc ttggcagcaa cctgggggag cgcatggagc
     3661 acctgcagcg cgccgtggac ctgctggacg acgacgcgcg cacccgcgtg gaggccgtct
     3721 ccagcgtcta cgagaccgac ccggtcggcg gccccgagca gggggcctac ctgaacgtcg
     3781 cgctgcgcgt ggcgacccgc cgctccccgc gcgggctact gcggctgtgc aa
//
LOCUS       DATMKC010000307         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_347562, whole genome shotgun sequence.
ACCESSION   DATMKC010000307 DATMKC010000000
VERSION     DATMKC010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347562"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..269
                     /locus_tag="VNU01_09085"
     CDS             141..269
                     /locus_tag="VNU01_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32809.1"
                     /translation="MDKLPTCPMCGSADTRVEEDVSDEVTVFGCNECGSTWADRND"
     gene            269..1876
                     /locus_tag="VNU01_09090"
     CDS             269..1876
                     /locus_tag="VNU01_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWH32810.1"
                     /translation="MDQLGTAADARSEAFQRNDEAHRLLVDDLRAQLATARLGGDERS
                     RLRHTERGKLLPRDRVDALLDPSSPFLELSPLAAHGLYDGDAPAAGIITGVGRVSGRE
                     CVIVANDATVKGGTYYPMTVKKHLRAQEVALQNRLPCVYLVDSGGAFLPKQDEVFPDR
                     EHFGRIFFNQAQMSARGIPQVAAVMGSCTAGGAYVPAMSDETVIVRNQGTIFLGGPPL
                     VKAATGEVVTAEELGGGDLHSRTSGVTDHLADDDAHALQIVRSIVSTLGAKPATPWDL
                     AASEEPAVDPAQLYGVVPPDTRTPYDVREVIARLVDGSRFHEFKALYGTTLVTGFARI
                     HGHPVGIVANNGILFSESALKGAHFIELCDQRGIPLVFLQNITGFMVGREYEAGGIAK
                     DGAKMVTAVSCANVPKLTVIVGGSYGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQA
                     ATVLATVRRDQLEARGEHWPADEEEAFKAPIRAQYEEQGHPYYSTARLWDDGVIDPMD
                     TRMVLGLALSACANAPVDPPRYGVFRM"
     gene            1929..>2358
                     /locus_tag="VNU01_09095"
     CDS             1929..>2358
                     /locus_tag="VNU01_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWH32811.1"
                     /translation="MFASSPLDRLFGNGPAKVLDALLRSDGLTVRQVSRVAGIAPGTA
                     SAALRRLERQWVVVGKPVGSALQYWVNEAHFAVGPLRDLVARAERLEASLPQLIVSIL
                     GTTPRSVILFGSVARSEADRDSDVDVLVVAHSEKELDWWRN"
BASE COUNT          354 a          796 c          859 g          349 t
ORIGIN      
        1 cggcacgccg aggccagcaa caccaggagc agcagcacag ccgcgcgcgt cgacaggtgt
       61 cgggctcgca tggccgccag ggtgccacgg gcgcgcgccg ctgtcctggc gcggcgcggg
      121 aaagccgtag gctttgcgcc atggacaagc tgcccacctg tcccatgtgc ggctccgcgg
      181 acacgcgcgt ggaagaggac gtgagcgacg aggtcaccgt cttcggctgc aacgagtgcg
      241 gttccacgtg ggccgacagg aacgactgat ggaccagctc ggcacggcgg cggacgcgcg
      301 ctcggaggcc ttccagcgca acgacgaggc acaccggctc ctggtcgacg acctgcgcgc
      361 gcagctggcc acggcgcgcc tcggcggcga cgagcgctcg cgcctgcgcc acaccgagcg
      421 cgggaagctc ttgccgcgcg accgcgtgga cgcgctgctg gacccgtcct cgccgttcct
      481 ggagctgtcg ccgctggccg cccacggcct gtacgacggc gacgcgcccg cggccgggat
      541 catcaccggc gtcgggcgtg tgagcggccg tgagtgcgtc atcgtggcca acgacgccac
      601 ggtgaagggc ggcacgtact acccgatgac ggtgaagaag cacctgcgcg cgcaggaggt
      661 ggcgctgcag aaccgcctgc cctgcgtgta cctggtggac tccggtgggg cgttcctgcc
      721 caagcaggac gaggtctttc cggaccgcga gcacttcggg cgcatcttct tcaaccaggc
      781 gcagatgtcc gcgcgcggca tcccgcaggt cgccgcggtg atgggctcgt gcaccgccgg
      841 cggcgcgtac gtcccggcga tgagcgacga gacggtgatc gtgcgcaacc agggcacgat
      901 cttcctgggc gggccgcccc tggtgaaggc ggccaccggc gaggtggtga ccgccgagga
      961 gctcggcggc ggggacctgc actcgaggac ttccggcgtg accgaccatc tcgccgacga
     1021 cgacgcgcac gccctgcaga tcgtgcgctc catcgtgtcg acgctgggcg ccaagcccgc
     1081 gacgccatgg gacctggcgg cgtccgagga gccggcggtc gaccccgcgc agctgtacgg
     1141 ggtggtgccg ccggacaccc gcacgcccta cgacgtgcgc gaggtgatcg cgcggctggt
     1201 cgatggcagc cggttccacg agttcaaggc gctgtacggc acgacgctgg tcaccggctt
     1261 cgcgcgcatc cacgggcacc ccgtcggcat cgtcgcgaac aacgggatcc tgttcagcga
     1321 gagcgcgctg aagggcgccc acttcatcga gctgtgcgac cagcgcggca tcccactggt
     1381 gttcctgcag aacatcaccg gcttcatggt gggccgcgag tacgaggccg gcgggatcgc
     1441 caaggacggc gccaagatgg tgaccgccgt gtcgtgcgcc aacgtgccca agctcacggt
     1501 gatcgtgggt ggcagctacg gcgccggcaa ctacgggatg tgcgggcgcg cgtactcgcc
     1561 ccggttcctg tggatgtggc ccaacgcgcg catctcggtg atgggcggcg agcaggcggc
     1621 gacggtgctg gccacggtgc gccgcgacca gctcgaggcg cgtggcgagc actggccggc
     1681 ggacgaggag gaggcgttca aggcgccgat ccgcgcccag tacgaggagc agggccaccc
     1741 gtactactcc accgcccggc tgtgggacga cggcgtgatc gaccccatgg acacgcgcat
     1801 ggtgctggga ctggcgctgt cggcctgcgc caacgcgccg gtggaccccc cgcgctacgg
     1861 cgtgttccgc atgtagcgca agtgtcctgt ctacttgaca agtgtccaat atattggaca
     1921 cttgtggaat gttcgcgtcc tcgccgctgg accggctctt cggcaatggc cccgcgaagg
     1981 tgctcgatgc cctgctgcgc tcagatggct tgaccgttcg ccaggtgtcc cgtgtggcgg
     2041 gcatcgcccc aggaaccgct tcggccgcgt tgcggcgcct tgagcggcaa tgggtggtgg
     2101 tgggcaagcc ggtgggctcg gccctgcagt actgggtcaa cgaagcccat ttcgccgttg
     2161 gcccgctgcg cgacctggtc gcacgtgcgg aacggctgga agcgagtctt ccacagttga
     2221 tcgtctcgat cctcggcacg acgccgcgat ccgtcattct gttcgggtcg gtcgctcggt
     2281 cggaagcgga tcgggacagc gacgtggacg tcctggtcgt cgcccactcg gagaaggaac
     2341 tggactggtg gaggaacc
//
LOCUS       DATMKC010000308         3181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_335849, whole genome shotgun sequence.
ACCESSION   DATMKC010000308 DATMKC010000000
VERSION     DATMKC010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335849"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VNU01_09100"
     CDS             complement(<1..315)
                     /locus_tag="VNU01_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWH32812.1"
                     /translation="MDAETERARRATPEESTEEILRAAEELLREAPLSALTVGAVMGR
                     TRLGRSSFYVYFRDVDDLVARLSQRLEEQLWEVTARWLDADDPGEGIEQALRALVDVW
                     AQH"
     gene            411..899
                     /locus_tag="VNU01_09105"
     CDS             411..899
                     /locus_tag="VNU01_09105"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH32813.1"
                     /translation="MPRFSNTIEIARPPEEVFAFLADHRNVPRWNYFVRTVRALDGTT
                     PALGARYHQVRRDDEQTFQITEWSPGRSFTIATLPGQRPAFERTMRFTSTPGGVRIDD
                     AWLLDIGQPRLLEPMLAQAARRGVGDNLARLRELLENGRTRLQDGRLSVLAGSELIRS
                     GG"
     gene            909..1640
                     /locus_tag="VNU01_09110"
     CDS             909..1640
                     /locus_tag="VNU01_09110"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HWH32814.1"
                     /translation="MRQAAVSLVLIAMLSACRAPAPEAPSASTAPEPDATVPPGALPS
                     PAPSPAAPTSPPAPTATEVRYRLDNRAVDSATAGFAGTVRAVLTDPRGWVRAGFRFVE
                     DADAPYRIVLAEGAEVDALCAPLETWSTYSCQNGPVVALNADRWRSATPQWPAGLDEY
                     RVMLVNHEVGHLLHLHHPRPQCPAAGLPAPVMAQQSTELGACLPNPWPLDWEVALAAE
                     RREPLAPPAEHDPAGHRPTAPPATS"
     gene            1725..>3181
                     /locus_tag="VNU01_09115"
     CDS             1725..>3181
                     /locus_tag="VNU01_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HWH32815.1"
                     /translation="MRKFVGGVSLALLLAVLPGGVSAHPDHAEDDGVLSPLHAGHQHG
                     ENDGHLPAVRRNVELVGKLDLFADAEQPGRVADVAVHGDHAYLGAFYEPECRHDSGGV
                     FVVDISDPANPRKAGFIPTTPYAYVGEGVQVLDLDTPSFTGQVLLHNNENCSPVPGGL
                     PAGRGGASLWDVTDPADPRPLAQHVGDTDVATTGGIPHLSSPHQSHSVFGWQQGEKAY
                     MVMVDNAELGNVGDEGFDIDIFDITDPANPVLAVETGLPDFPEIVEDPSPNGNNAFLH
                     DMVVKEVDGRFLLLGSYWDGGYIVLDVTDLPAKPVFLRDTDFGSSEPFAAEMGLPADW
                     TPEGNAHQAEFNHDNSLFLGADEDFNAHRVTGTIAGGTYQGETFSATQGSDTPPITPE
                     TPMAGPTQFLGEACGPVLPSTEEGEIALVVRGTCTFTIKVQTAERAGYDGVIVFNDQQ
                     GGAGCEAQVNMLAVGEIPSMFVARSVGLKLLDVSP"
BASE COUNT          447 a         1202 c         1092 g          440 t
ORIGIN      
        1 atgctgcgcc cacacgtcca cgagcgcgcg gagtgcctgc tcgatcccct cgcccggatc
       61 gtcggcgtcc agccagcgcg cggtcacctc ccagagctgc tcctccagtc gctgcgacag
      121 ccgcgcgacg aggtcgtcga cgtcccggaa gtacacgtag aaggacgacc gtcccaggcg
      181 cgtgcgcccc atgacggcgc ccaccgtgag cgcggacagc ggcgcctccc gcagcagttc
      241 ctcggcggcc cgcaggatct cctccgtcga ctcctcgggc gtggcgcgcc gcgcacgctc
      301 cgtctccgcg tccatcgggg ctcctgtcgt tgttgacgca ccgtcagtcg tgtgcttgac
      361 tgacactctg tcattgactc actgtcaata ctaccgggaa ggacgcccgc atgccgcgct
      421 tcagcaacac catcgagatc gccaggccgc cagaggaggt cttcgcgttc ctcgccgatc
      481 atcgcaacgt gccgcgctgg aactacttcg tgcgcaccgt ccgggcgctg gacggcacca
      541 cgcccgcact cggcgcgcgc taccaccagg tgcgccgtga cgacgagcag accttccaga
      601 tcaccgagtg gtcccccggc cggtcgttca ccatcgccac gctgcccggc cagcgcccgg
      661 cgttcgagcg cacgatgcgt ttcacgtcca cgcccggcgg cgtgcgcatc gacgacgcct
      721 ggctgctcga catcggccag ccgcggctgc tggagcccat gctggctcag gccgcgcgcc
      781 ggggtgtcgg ggacaacctg gcacggctgc gcgagctgct cgagaacggc cgcacccgtc
      841 tccaggacgg ccgcctcagc gtgctggcgg gcagcgagct gatccgctct gggggttagg
      901 ctgcccccgt gcgccaggca gcggtgtccc tggtcctcat cgccatgctg tccgcctgcc
      961 gggcaccggc accggaggct ccctcggcga gcactgcgcc ggagccggat gcgaccgtgc
     1021 caccaggcgc ccttccgtcg ccggcgccgt cgcccgcggc gccgacgtca cctccggcgc
     1081 ccacggcgac cgaggtgcgc taccggctgg acaaccgcgc cgtggacagc gcgaccgcgg
     1141 gcttcgccgg cacggtgcgc gccgtgctga ccgacccccg ggggtgggtg cgcgcgggct
     1201 tccgcttcgt cgaggacgcg gacgcgccgt accgcatcgt cctggccgaa ggcgcagagg
     1261 tggacgcgct gtgcgcgccg cttgagacct ggtcgaccta ctcctgccag aacgggccgg
     1321 tggtcgcgct caacgccgac cgctggcggt ccgcgacgcc ccagtggccg gcaggcctgg
     1381 acgagtaccg cgtgatgctg gtcaaccacg aggtcggcca cctgctgcac ctgcaccacc
     1441 cgcggccgca gtgcccggcg gccgggctgc ccgcgccggt catggcgcag cagagcaccg
     1501 agctgggcgc ctgcctgccg aacccgtggc cgctggactg ggaggtcgcg ctggccgccg
     1561 agcggcgaga gccgctggcg ccgcccgcgg agcacgaccc ggccggccac cgccccaccg
     1621 cgccgccggc gacgtcctga tcgccgtttt tcacggggcg cgcgttttgc cggatgcgtc
     1681 tcgtggtacg cccgcctcca cgaaaccgag gtggggaggc tcgcatgcgc aagttcgtgg
     1741 gtggggtgtc gctggcgttg ctgttggcgg tgttgcccgg aggcgtgtcc gcgcacccgg
     1801 accacgccga ggacgacggc gtgctgtcgc cgctgcacgc cggccatcag cacggcgaga
     1861 acgacggcca tctgcccgcg gtgcggcgca acgtcgagct ggtcggcaag ctcgacctgt
     1921 tcgccgacgc cgagcagccc ggccgcgtcg cggacgtcgc ggtccacggc gaccacgcct
     1981 acctgggcgc gttctacgag ccggagtgcc gtcacgacag cggcggcgtg ttcgtggtcg
     2041 acatctccga cccggccaac ccgcgcaagg ccggcttcat ccccaccacg ccgtacgcct
     2101 acgtcggtga gggcgtgcag gtgctcgacc tggacacgcc gtcgttcacc ggccaggtcc
     2161 tgctgcacaa caacgagaac tgctcgcccg tgccgggcgg cctgcccgca ggacggggcg
     2221 gcgcgtccct gtgggacgtg acggacccgg ccgacccgcg gccgctggcc cagcacgtcg
     2281 gcgacacgga cgtcgccacc accggcggca tcccgcacct gtcctcgccg caccagagcc
     2341 acagcgtgtt cgggtggcag cagggcgaga aggcctacat ggtcatggtc gacaacgccg
     2401 agctcggcaa cgtcggcgac gagggcttcg acatcgacat cttcgacatc accgaccccg
     2461 cgaacccggt gctcgccgtg gagaccggcc tgccggactt ccccgagatc gtcgaggacc
     2521 cctcccccaa cggcaacaac gcattcctgc acgacatggt ggtcaaggag gtggacgggc
     2581 gcttcctgct gctcggctcc tactgggacg gcggctacat cgtcctcgac gtgacggacc
     2641 tgcccgcgaa gccggtcttc ctgcgcgaca ccgacttcgg ctcgagcgag ccgttcgccg
     2701 cggagatggg cctgccggcg gactggaccc cggagggcaa cgcgcaccag gcggagttca
     2761 accacgacaa cagcctgttc ctgggcgccg acgaggactt caacgcccac cgcgtcaccg
     2821 gcacgatcgc cgggggcacc taccagggtg agacgttctc ggccacgcag ggcagcgaca
     2881 cgccgccgat caccccggag acgccgatgg cggggccgac ccagttcctc ggcgaggcct
     2941 gcggcccggt gctgccgtcc accgaggagg gcgagatcgc cctggtggtg cgcggcacct
     3001 gcaccttcac catcaaggtg cagaccgccg agcgcgccgg ctacgacggg gtgatcgtgt
     3061 tcaacgacca gcagggcggc gccggctgcg aggcgcaggt caacatgctg gccgtcggcg
     3121 agatcccgtc gatgttcgtg gctcggtccg tgggcctgaa gctgctcgac gtctcgccga
     3181 t
//
LOCUS       DATMKC010000309         9474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_146063, whole genome shotgun sequence.
ACCESSION   DATMKC010000309 DATMKC010000000
VERSION     DATMKC010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146063"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VNU01_09120"
     CDS             <1..1263
                     /locus_tag="VNU01_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH32816.1"
                     /translation="EDWEVSDDQLTWTFTLRDDVTWHDGEPFTADDVVHSLNRIKEEG
                     QNAWRLSAVEQITAADDHTVEIQVAQPTPNLLANLGAFKGMAIVPEHVDPAELAQEPV
                     GTGPFSFGSSQSGSSITLEANPDYWGEGPNVEAVEFRFIPEGSTAMANLRAGDVHWTD
                     NIPPQDVARVLEGDDIQAEAVPSNDYWYVAFNQNREPFGDVNVRRALAMAIDREAVTQ
                     AAKFDAATVNQTAIPQDSFWHYDYAPFERDVEEARRLLQEAGAEGLTIDLMVTSEYEE
                     TIQAAQVLEANWREIGVDTNIRTLDFSAWLAEQGEGNFDAFMLGWLGNIDPDDFYYAQ
                     HHTGANFNFHGYSNAEVDRLLDEARTETDQDARKELYDQAVKLIVDEVSYAYLYNPDV
                     VHAWTPQVSGYTVRPDRAIRFKDVALES"
     gene            1285..2226
                     /locus_tag="VNU01_09125"
     CDS             1285..2226
                     /locus_tag="VNU01_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH32817.1"
                     /translation="MARYVVRRTAQAAVVLLGVSVLVFLLVHLVPGDPIRTAMGPRYD
                     PEVYQRLSERAGFDQPLPVQYGQWLAGAVTGDLGVSFRTGRPVMGALLERLPATLTLA
                     FASVVVALLIALPLGVLSAVRSGSKLDYAATVFSQVGISIPDFWMGILFILVFAEYLQ
                     ILPASGYASLLDDPVAWLRHLLMPAVTIGVVSGSILTRFVRSSVLESLGQDYVRTARA
                     KGLSERVVVTRHVLRNALIPFVTVTGLQLATLLGGVIIVEFVFAWPGLGLLALQAVDA
                     RDYTVLQGAVLLIATLFLLINLVVDLLYAWLDPRIKY"
     gene            2226..3122
                     /locus_tag="VNU01_09130"
     CDS             2226..3122
                     /locus_tag="VNU01_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH32818.1"
                     /translation="MGAVAVSRQQRWLAFLRVVVANRLAAAGLVVIVLLALAAIFGEF
                     LAPYPPSDLDVTSRLQPPSGAHPFGTDELGRDVLSRVIVGARVSVQVAAIAVGISLVA
                     GVLTGLVAGYYGGRVDTVLMRLMDVLFAFPAILLAIAILAVLGPGLTNAMIAIGVVFT
                     PIFARITRASVLSVREEVYVRAARSLGASDARLIALHVLPNVLAPVIVQTSISLAFAI
                     LNEAALSFLGLGVQPPRPSWGRMLFEGRGFVRQAWWMAVFPGAAIFVTVLSFNVLGDA
                     LRDALDPRQKSAIEARGGGGPA"
     gene            3119..4111
                     /locus_tag="VNU01_09135"
     CDS             3119..4111
                     /locus_tag="VNU01_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32819.1"
                     /translation="MSEPLLEVADLRIRFHTRRGVASVVNGVTWSVRPGETVAIVGES
                     GSGKSVSTLAILGLVPQPPGEVSGSVRFEGRELVGASDADLRRMRGNDIAMVFQDPMT
                     SLNPVLTVGRQITEALELHLGLRGDAARRRAAELLDQVGIPSPDRRLDSYPHQMSGGM
                     RQRVMIAIALACDPKVLIADEPTTALDVTIQAQIVELIDGLQAERGMGVVWITHDLGV
                     VAGLADRVLVMYGGQVVEEAPVSRLYASPAHPYTQGLLAALPVLGEHRDELQTIAGLP
                     PDPSDLPPGCLFWPRCPKRLDARCQTERPVLRPAGPDHRVATFYDAGSEVASGA"
     gene            4101..5159
                     /locus_tag="VNU01_09140"
     CDS             4101..5159
                     /locus_tag="VNU01_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32820.1"
                     /translation="MAPEPGDELLRVEDLRVHFPAGGRALGKGRDVVRAVDGVSLSVR
                     RGETLGLVGESGSGKTTTGLALLRLVEPTSGRVWFDGTDVTALRRRDLRAFRRRVGMV
                     FQDPFASLNPRRSVGASVAEPMEIHGLHREGRDRRVAELFEMVGLDPALRRRYPHEFS
                     GGQRQRIGIARALAAEPDFLVLDEPIASLDVSIQAQVVNLLRSLQRELGLTYLFIAHD
                     LAAVQHISDRIAVLYLGVVMESGARDAVVGEPQHPYTRSLVSAVPIPDPERERRRERI
                     VLVGDVPSPLDPPSGCNFRTRCPDVFAPCGTVEPLLQDTPNGNLAACHLHGVVGREVP
                     RSHHPDSTQVAEAEGRVG"
     gene            5171..6034
                     /locus_tag="VNU01_09145"
     CDS             5171..6034
                     /locus_tag="VNU01_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HWH32821.1"
                     /translation="MRSSSPLRHFSQGLLMGAADIVPGVSGGTIALIVGIYERVVDSI
                     RALAKGKVRDVEWALVLPLGVGVLIALGVGSAFIPALLERYPAPTRAVFFGFIAGSVP
                     IPWSQIREPDGRRWALAAVGAVAAFFLVGLPARDLADPSVPRVFASAMVAICAMILPG
                     VSGAFLLLVLGLYEHTLRALSDLDVGYIVTFGLGAVVGLALFSRVLGYLLDHRHDATM
                     AVLVGLMAGSLRMLWPWLGEDRGLLPPPDAGDVLVGLALAAIGFVVVRGLLRLQAEAP
                     EDWHTPPAEPG"
     gene            complement(6103..6618)
                     /locus_tag="VNU01_09150"
     CDS             complement(6103..6618)
                     /locus_tag="VNU01_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6343..6345),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HWH32822.1"
                     /translation="MDRYVSPIDERTLEPLARAPRLASLDGAVIGLLDINKPRGEQFL
                     NRVEELLRERGVAEVVRLRKPTFTRPAPDDVISLADRCGAVVEALADUGSCVSCGSHD
                     VVTLEARGIPAVLVGTEPFRHEALEQVRVLGMPDVRMVELAHPIQPLPPETVAALADG
                     VLDEIVAALTQ"
     gene            complement(6632..8140)
                     /locus_tag="VNU01_09155"
     CDS             complement(6632..8140)
                     /locus_tag="VNU01_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32823.1"
                     /translation="MGTAPPFTLTDTSGAVHRLDALHASGPLLAVFAERDCPTSAAAL
                     AALRDTGGNLVVVSEGSPEAARELAGLAGGRTVLVERAPYPVSAAYGLQTVPTFVLVS
                     AGGDEAERAEGWDAEAVARMLEAAGGDPGRIATDLPSLKPGCQARNTYDEATGLRLEA
                     EDRAALASDGVDEMWERGWHDGLPTVPPTRERVRAMLGGRDPGEELGKVPPAMGLLTM
                     ERLAACAVLAGCDPSYFPVVEAVARAALEPAFNLHGMQNTTHPAAPAIVVNGPVRERL
                     GMNGGSNALGFGNRANATIGRALRLVMALTGGGTPGGLDQSTLGGPHKWTLCFPERQE
                     ASPWDPLHVDKGFAAGESVVSLYCTEGPAMISDHYSQSADQLAATLALGLAGVWAGAW
                     YPLAAETVLVLCTEHAKTFGDAGWSKQQVRERLFELSKRSVGELKATGSGELTPLITY
                     AQDDGAVVSKFLSPEEIVVVVAGSDAGRFSAVLAPWAGFGLGSTPVSRRVEA"
     gene            8205..8621
                     /locus_tag="VNU01_09160"
     CDS             8205..8621
                     /locus_tag="VNU01_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32824.1"
                     /translation="MIQVLIDADNLTAPRLRALLRALPADECRILVVGSPAALAQAEW
                     PREARVVEAVGWQAADLILAEAYEHGDEPLVLASGDGDFGAVVAGHRGPVLVVSDRPA
                     SMLRRAATVLDPVQDGLGALRDWFDAVLDGPDATAT"
     gene            8900..>9474
                     /locus_tag="VNU01_09165"
     CDS             8900..>9474
                     /locus_tag="VNU01_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32825.1"
                     /translation="MQGGLAPAGPVAAEIESLWWLMFWLGLAVFAVFAGALAHAVWRG
                     RREREERGGVAGIPAEEPAESLRFVRGSIIGLGVVMNGAVLLVVLVATVLAMRAIPDR
                     ADAAGLTLDLEARQFAWRATYPNGATTDGEVRIPAGEPVALRLTSADVIHSFWVPALA
                     GKLDALPDGVSTLVLQADEPGTYRGQCAEFCG"
BASE COUNT         1170 a         3461 c         3479 g         1364 t
ORIGIN      
        1 gaggactggg aggtctccga cgaccagctc acgtggacgt tcacgctgcg cgacgacgtc
       61 acctggcacg acggcgagcc gttcacggcg gacgatgtcg tgcactccct gaaccgcatc
      121 aaggaggagg gccagaacgc ctggcgcctg tcggccgtcg agcagatcac cgccgccgac
      181 gaccacaccg tcgagatcca ggtggcccag cccacgccga acctgcttgc caacctgggc
      241 gccttcaagg gcatggcgat cgtgccggag cacgtcgacc cggccgagct ggcgcaggag
      301 ccggtgggca ccgggccgtt cagcttcggc tcgtcgcagt ccggcagctc catcacgctg
      361 gaggccaacc cggactactg gggggagggg cccaacgtcg aggcggtgga gttccgcttc
      421 atccccgagg gctccacggc catggcgaac ctgcgcgccg gcgacgtgca ctggaccgac
      481 aacatcccgc cgcaggatgt cgcgcgtgtc ctcgagggcg acgacatcca ggccgaggcg
      541 gtgcccagca acgactactg gtacgtcgcg ttcaaccaga accgggagcc gttcggcgac
      601 gtgaacgtgc gccgggcgct ggcgatggcg atcgaccgcg aggccgtgac gcaggcggcg
      661 aagttcgacg ccgcgaccgt caaccagacc gccatcccgc aggacagctt ctggcactac
      721 gactacgcgc cgttcgagcg cgacgtcgag gaggcgcgcc ggctgctgca ggaggccggg
      781 gccgagggcc tgacgatcga tctcatggtg acctcggagt acgaggagac catccaggcc
      841 gcgcaggtgc tggaggcgaa ctggcgcgag atcggcgtgg acaccaacat ccgcacgctg
      901 gacttctccg cgtggctggc cgagcagggc gagggcaact tcgacgcctt catgctgggc
      961 tggctcggca acatcgaccc ggacgacttc tactacgccc agcaccacac cggcgcgaac
     1021 ttcaacttcc acggctactc caacgccgag gtcgaccggc tgctggacga ggcgcgcacg
     1081 gagaccgacc aggacgcccg caaggagctg tacgaccagg cggtgaagct catcgtggac
     1141 gaggtgagct acgcctacct gtacaacccc gacgtcgtgc acgcctggac cccgcaggtg
     1201 tccggctaca cggtgcgccc ggaccgggcg atccgcttca aggacgtcgc actggagtcc
     1261 taggccgggc gccggggcag gaggttggcg cgctacgtcg tccgcaggac cgcgcaggcg
     1321 gcggtcgtgc tgctgggcgt cagcgtgctg gtgttcctgc tggtgcacct ggtgcccggc
     1381 gacccgatcc gcacggcgat ggggccccgc tacgaccccg aggtgtacca gcgcctgagc
     1441 gagcgcgcgg gcttcgacca gccgctgccg gtgcagtacg gccagtggct ggccggtgcg
     1501 gtgaccggtg acctgggggt gagcttccgc acgggccgcc cggtcatggg cgcgctgctg
     1561 gagcgcctgc cggccacgct cacgctggcg ttcgcgtccg tggtggtggc gctgctgatc
     1621 gcgctgccgc tgggcgtgct gtcggcggtg cgctcgggct ccaagctgga ctacgccgcg
     1681 acggtgttca gccaggtggg catcagcatc ccggacttct ggatgggcat cctgttcatc
     1741 ctggtgttcg ccgagtacct gcagatcctg ccggcctccg gctacgcgtc cctgctcgac
     1801 gacccggtgg cgtggctgcg ccacctgctg atgccggcgg tgaccatcgg cgtggtcagc
     1861 gggtccatcc tcacgcgctt cgtgcgctcc tcggtcctgg agtccctggg ccaggactac
     1921 gtccgcaccg cgcgcgccaa gggcctgagc gagcgcgtcg tggtcacgcg ccacgtgctg
     1981 cgcaacgcgc tgatcccgtt cgtgaccgtc acggggctgc agctcgcgac gctgctcggc
     2041 ggcgtgatca tcgtcgagtt cgtgttcgcc tggccgggcc tgggcctgct ggcgttgcag
     2101 gccgtcgacg cgcgcgacta cacggtgctg cagggcgcgg tgctgctgat cgcgacgctg
     2161 ttcctgctga tcaatctcgt cgtggacctg ctgtacgcgt ggctcgaccc caggatcaag
     2221 tactgatggg cgccgtggcg gtgtcgcggc agcagcgctg gctggcgttc ctgcgcgtcg
     2281 tcgtggccaa ccgcctggcc gccgccggcc tggtggtcat cgtgctgctg gcgctggccg
     2341 cgatcttcgg cgagttcctg gcgccctacc cgcccagcga cctcgacgtc accagccgcc
     2401 tgcagccgcc gtcgggcgcg cacccgttcg gcaccgacga gctgggccgc gacgtcctgt
     2461 cgcgcgtgat cgtgggcgcg cgcgtgtccg tgcaggtcgc cgccatcgcg gtcggcatct
     2521 cgctggtggc cggcgtgctg accggcctgg tggccggcta ctacggcggc cgcgtcgaca
     2581 ccgtgctgat gcgcctcatg gacgtgctgt tcgcgttccc ggcgatcctg ctcgccatcg
     2641 cgatcctggc cgtgctcggc cccgggctga ccaacgcgat gatcgcgatc ggcgtggtgt
     2701 tcacgccgat cttcgcgcgc atcacccgcg ccagcgtgct cagcgtgcgc gaggaggtgt
     2761 acgtgcgcgc ggcccgctcc ctgggtgcct cggacgcccg cctcatcgcg ctgcacgtgc
     2821 tgccgaacgt gctggccccc gtgatcgtgc agacgtccat cagcctggcg ttcgcgatcc
     2881 tcaacgaggc ggcgctgtcg tttttggggc tgggcgtgca gccgccgcgg ccctcctggg
     2941 gccgcatgct gttcgagggg cgcggcttcg tgcggcaggc ctggtggatg gcggtgttcc
     3001 ccggggcggc gatctttgtg acggtgctgt cgttcaacgt gctgggcgac gcgctgcgcg
     3061 acgccctgga cccgcggcag aagtcggcca tcgaggcgcg cggcggcggg ggcccggcat
     3121 gagcgagccg ctgctggagg tcgccgacct gcgcatccgc ttccacaccc ggcggggcgt
     3181 ggccagcgtg gtcaacggcg tcacgtggtc ggtgcgaccg ggggagaccg tggccatcgt
     3241 cggcgagtcc ggcagcggca agagcgtgtc caccctggcc atcctggggc tggtgcccca
     3301 gccgcccggc gaggtgagcg gctcggtgcg cttcgagggc cgcgagctgg tgggcgcgag
     3361 cgacgccgac ctgcggcgca tgcgcggcaa cgacatcgcg atggtgttcc aggaccccat
     3421 gacgtcgctc aacccggtgc tcacggtcgg gcgccagatc accgaggcgc tggagttgca
     3481 cctgggcctg cgcggggacg cggcccggcg gcgcgccgcg gagctgctgg accaggtcgg
     3541 catcccctcg cccgaccgcc gcctggacag ctacccgcac cagatgtccg gcggcatgcg
     3601 ccagcgcgtg atgatcgcca tcgcgctggc ctgcgacccc aaggtgctga tcgccgacga
     3661 gcccaccacg gccctcgacg tcaccatcca ggcgcagatc gtggagctga tcgacggcct
     3721 gcaggccgag cgcggcatgg gcgtggtgtg gatcacccac gacctgggcg tggtggcggg
     3781 cctggccgac cgcgtgctgg tgatgtacgg cggccaggtg gtggaggagg cgccggtgag
     3841 ccgcctgtac gccagccccg cgcatcccta cacccagggc ctcctggccg cgctgcccgt
     3901 gctgggcgag caccgcgacg agctgcagac catcgccggc ctgccgcccg acccgtcgga
     3961 cctgccgccc gggtgcctgt tctggccgcg ctgccccaag cgcctggacg cccgctgcca
     4021 gacggagcgg ccggtgctgc ggccggccgg cccggaccac cgcgtggcga ccttctacga
     4081 cgccggctcg gaggtggcca gtggcgcctg agcccggaga cgagctgctg cgcgtggagg
     4141 acctgcgcgt gcacttcccg gcgggcgggc gcgcgctggg caagggccgc gacgtcgtgc
     4201 gcgccgtcga cggcgtcagc ctgtccgtgc gccgtggcga gacgctcggc ctggtggggg
     4261 agtccggttc cggcaagacc accaccggcc tggcgctgct gcgcctggtg gagccgacca
     4321 gcggccgggt gtggttcgac ggcacggacg tcaccgcgct gcgccgccgc gacctgcgcg
     4381 cgttccgcag gcgcgtgggc atggtgttcc aggacccgtt cgcgtcgctc aacccgcggc
     4441 gcagcgtggg tgcgagcgtc gccgagccca tggagatcca cggcctgcac cgcgaagggc
     4501 gcgaccgccg ggtggccgag ctgttcgaga tggtgggcct ggacccggcg ctgcggcggc
     4561 gctacccgca cgagttctcc ggcggccagc gccagcgcat cggcatcgcc cgcgcgctgg
     4621 cggccgagcc ggacttcctg gtgctcgacg agccgatcgc gtcgctcgac gtctccatcc
     4681 aggcgcaggt ggtgaacctg ctgcgctcgc tgcagcgcga gctgggcctg acgtacctgt
     4741 tcatcgcgca cgacctcgcg gcggtgcagc acatcagcga ccgcatcgcg gtgctgtacc
     4801 tgggcgtggt gatggagagc ggcgcgcgcg acgcggtggt gggcgagccg cagcacccgt
     4861 acacgcgctc gctggtgtcg gccgtgccga tccccgaccc ggagcgcgag cggcggcgcg
     4921 agcgcatcgt cctggtgggc gacgtgccga gcccgctgga cccgcccagc ggctgcaact
     4981 tccgcacgcg ctgcccggac gtgttcgcac cgtgcggaac cgtggagccg ctcctgcagg
     5041 acacgccgaa cggcaacctg gccgcatgcc acctgcacgg cgtggtcggc cgggaggtcc
     5101 cgcgctcgca ccatccggac agcacccagg tggccgaggc ggagggccgg gtcggatagc
     5161 ctgcgccccc atgcgctcgt cgtcaccgct gcggcatttc agccagggcc tgctcatggg
     5221 ggccgccgac atcgtccccg gcgtcagcgg cggcaccatc gccctgatcg tcggcatcta
     5281 cgagcgggtc gtggactcca tccgcgcact ggcgaagggc aaggtgcgcg acgtcgagtg
     5341 ggcgctggtg ctgcctctcg gcgtcggggt gctgatcgcg ctcggcgtcg gctccgcgtt
     5401 catcccggcg ctgctcgagc gctacccggc ccccacgcgc gccgtcttct tcggcttcat
     5461 cgccggttcc gtcccgatcc cgtggagcca gatccgcgaa ccggacgggc gccgctgggc
     5521 gctggcggcg gtcggcgcgg tggcggcgtt cttcctggtg ggcctgccgg cgcgcgacct
     5581 ggccgacccg tcggtgccgc gcgtgttcgc ctcggcgatg gtggcgatct gcgccatgat
     5641 cctgccgggg gtgagcgggg cgttcctgct gctggtgctg gggctgtacg agcacacgct
     5701 gcgggcgctg agcgacctgg acgtcggcta catcgtcacg ttcggcctgg gcgcggtcgt
     5761 cggcctggcg ctgttctcgc gcgtgctcgg ttacctgctg gaccaccgcc acgatgcgac
     5821 catggcggtg ctggtggggc tcatggccgg ctcgctgcgc atgctgtggc cgtggctggg
     5881 cgaggatcgc ggcctgctgc cgccgccgga cgccggcgac gtgctggtcg gcctggcgct
     5941 cgcggcgatc ggcttcgtgg tggtccgggg gctgctgcgc ctgcaggccg aggcgcccga
     6001 ggactggcac accccacccg ccgagcccgg ctgacccagg ccgacccggg gtgtgtcgac
     6061 tcagtcaatg tcaggatgat tcagtcgaca caccccgggt ggtcactgcg tgagtgccgc
     6121 gacgatctcg tccagcacgc catcggccag cgcggcgacg gtttccggcg gcagcggctg
     6181 gatcgggtgc gccaactcca ccatccgcac gtccggcatg ccgagcacgc gcacctgctc
     6241 cagcgcctcg tggcggaacg gctccgtgcc gaccagcacg gcggggatgc cccgggcctc
     6301 caaggtcacg acgtcgtgcg aaccgcacga cacgcagctg cctcagtcgg ccagcgcctc
     6361 caccacggcg ccgcagcggt cggccagcga gatcacgtcg tccggcgcgg gccgcgtgaa
     6421 cgtcggcttg cgcagccgca ccacctcggc gacgccgcgc tcgcgcagca gctcctcgac
     6481 gcgattcagg aactgctcgc cgcgcggctt gttgatgtcc agcaggccga tcacggcgcc
     6541 gtccagcgac gccagccgcg gggcgcgcgc caggggctcg agcgtgcgct cgtcgatcgg
     6601 gctcacgtag cggtccatgc tcactcctgc ctcacgcctc gacccgccgg ctcaccggcg
     6661 tgctgcccag cccgaacccg gcccacggcg cgagcaccgc gctgaagcgg cccgcgtcgc
     6721 tgcccgcgac cacgaccacg atctcctccg gtgacaggaa cttcgacacc accgcgccgt
     6781 cgtcctgcgc gtaggtgatc agcggcgtca gctcgccgga gccggtggcc ttgagctcgc
     6841 cgacgctgcg cttgctcagc tcgaacagcc gctccctgac ctgctgcttg ctccagccgg
     6901 cgtcgccgaa ggtcttggcg tgctcggtgc acagcaccag caccgtctcg gccgccagcg
     6961 ggtaccaggc gcccgcccac accccggcca gccccagcgc cagggtcgcg gcgagctggt
     7021 cggcggactg cgagtagtgg tcgctgatca tggccggccc ctcggtgcag tacagcgaca
     7081 ccacggactc gccggccgcg aagcccttgt ccacgtgcag ggggtcccac ggcgaggcct
     7141 cctggcgctc cgggaagcac agggtccact tgtgcggccc gcccagcgtg gactggtcca
     7201 ggccgcccgg cgtgccgccg ccggtcaggg ccatgaccag ccgcagggcg cgcccgatgg
     7261 tggcgttcgc gcggttgccg aaacccaggg cgttggagcc gccgttcatg ccgaggcgct
     7321 cgcggacggg gccgttcacc acgatggccg gcgcggcggg gtgggtggtg ttctgcatgc
     7381 cgtgcaggtt gaacgccggc tccagcgccg cgcgcgccac cgcctccacc accgggaagt
     7441 acgacgggtc gcagccggcc agcaccgcgc aggcggccag gcgctccatc gtcaaaagcc
     7501 ccatggcggg cggcaccttg cccagctcct cgccggggtc gcggccgccc agcatggcgc
     7561 gcacgcgctc gcgcgtcggc ggcaccgtgg gcaggccgtc gtgccagccg cgctcccaca
     7621 tctcgtcgac gccatcggac gccagcgccg cacggtcctc ggcctccagg cgcaggccgg
     7681 tcgcctcgtc gtaggtgttg cgtgcctggc agcccggctt gagcgacggc agatccgtcg
     7741 cgatgcgccc cgggtcgccg cccgctgcct ccagcatgcg cgcgaccgcc tcggcgtccc
     7801 agccctcggc gcgctccgcc tcgtcgccgc cggcggagac gagcacgaag gtcggcacgg
     7861 tctgcagtcc gtaggcggcc gacaccgggt agggcgccct ctccaccagc acggtgcgcc
     7921 cgccggccag ccccgccagc tcgcgcgccg cctcgggcga tccctcggac acgaccacca
     7981 ggttgccgcc cgtgtcgcgc agggcggcca gcgccgccgc ggaggtgggg cagtcgcgct
     8041 ccgcgaacac ggccagcagc ggcccggagg cgtgcaaggc gtcgaggcga tgcaccgcac
     8101 cggaggtgtc ggtgagcgtg aacggcggcg cggtgcccat ccagccctcc cgcgtaggct
     8161 tgcgatccga gtcggctccg gcagggacca tagatgatgc agggatgatc caggtgctca
     8221 tcgacgcgga caacctcacc gcgccgcggc tgcgcgcgct gctgcgggcc ctgccggccg
     8281 acgagtgccg gatcctggtg gtgggcagtc ccgccgcgct ggcgcaggcc gagtggccgc
     8341 gcgaggcgcg cgtggtcgag gcggtcgggt ggcaggccgc ggacctcatc ctggccgagg
     8401 cctacgagca cggcgacgag ccgctggtgc tcgcctccgg cgacggcgac ttcggcgccg
     8461 tcgtggccgg ccaccgcggg ccggtgctgg tcgtgagcga ccgtcccgcc tcgatgctgc
     8521 gccgcgccgc caccgtgctc gacccggtgc aggacggtct gggcgccttg cgcgactggt
     8581 tcgacgccgt gctggacggg ccggacgcga cggcgacgtg aaccgcaacg gcgacgtagc
     8641 cctcgggaaa cgccgccgtc atgccgatac gtcaaggtga ggcgtattgc ccggcgtggc
     8701 tgccgggtga agggaggagg aggtccatgc ggcccacaca gctctccaag ccttgcccct
     8761 ggctcgcccg cggtgaccgc tgcccgttcg gctgcacggc cgccgaccac ccccacgccg
     8821 cgcccgcctg agccgcagcc gccgtttcct gcccgcgccc gccgggtagc gcctcctccc
     8881 gagaccggga ggcgcgcgca tgcaaggagg ccttgctccc gccggccccg tggccgcgga
     8941 gatcgagtcg ctgtggtggc tgatgttctg gctgggcctg gcggtgttcg ccgtcttcgc
     9001 cggggcgctg gcgcacgcgg tgtggcgcgg gcggcgcgag cgcgaggagc gcggcggcgt
     9061 cgccggcatc ccggccgagg agcccgccga gtcgctgcgc ttcgtgcgcg gctcgatcat
     9121 cggcctgggc gtggtcatga acggtgccgt gctcctggtg gtcctggtcg ccaccgtgct
     9181 cgcgatgcgc gccatccccg accgggcgga cgccgccggg ctgaccctgg acctggaggc
     9241 gcgccagttc gcgtggcggg ccacctaccc caacggcgcg acgacggacg gcgaggtgcg
     9301 catcccggcc ggcgagccgg tcgcgctccg cctcacgtcc gccgacgtga tccacagctt
     9361 ctgggtgccg gcgctggccg gcaagctcga cgcgctgccc gacggggtca gcacgctggt
     9421 gctgcaggcc gacgagccgg gcacctatcg cgggcagtgc gccgagttct gcgg
//
LOCUS       DATMKC010000310         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_74760, whole genome shotgun sequence.
ACCESSION   DATMKC010000310 DATMKC010000000
VERSION     DATMKC010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74760"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VNU01_09170"
     CDS             complement(<1..1098)
                     /locus_tag="VNU01_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWH32826.1"
                     /translation="MSPSTHRSVLRRGMAVLWAYIRMEPRSFAAAVAGAALYAATTVG
                     SAVVVGRVTDHVVLPSLEAGRVRTGALAAGALTIFAVIVLKGVGVVLRRTAATLMQFR
                     LEARHRREVSSRYLRLPLSWHRRHSTGELLSTAHADVEAAFGAIAPLPFSAGVVLMLA
                     IAAAVMVLTDPLLAVIGLAMGAVMTALNWSYNRRVGGVAIRAQQLRGEVSEVAHESFD
                     GALVVKTLGREAAETERFRQSSERLRDELVRMGRLRATFDPSMEVLPELGILAVLLLG
                     GWRVSTGAATPGDVVQFAYLFSLLAFPLRVLGFLLGDLPRSVAGWERVRGVLDAREEL
                     LEAAGAGAAPPEAVPEAGQRAPARASELELRG"
     gene            1214..2200
                     /locus_tag="VNU01_09175"
     CDS             1214..2200
                     /locus_tag="VNU01_09175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWH32827.1"
                     /translation="MTLALSLPITGMPLDETVAYVRDCAELGYRAVWGSEVAGPDFAT
                     LLGAVAGAGLDVDLGVAVIPVQTRSPWLIAATAASLAQMSGGRFSLGLGTSSELIAGQ
                     WSGAPFDKPLTHLRETVETVRAILAGERANVDGEYVRTHGYKLFAPTPPVPIIVGALN
                     AASLRQAGRIGDGVALNQSAPEHLPMILGEVRKGAEEAGKDFAAMPVVSRIFCMVTDQ
                     PAAGREMVRHIFAPYAATSVYNRFFRWLGFTEEMEAVAEAFGRKDRAAAAAALSDRFV
                     ESIYVIGDADHVAQRVKDYVDNGVTVAAIQPLAAGRDDALRTMKAIAEAYGL"
     gene            2278..>2599
                     /locus_tag="VNU01_09180"
     CDS             2278..>2599
                     /locus_tag="VNU01_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWH32828.1"
                     /translation="MNETSAPPADAIPPPDEPPPPQTGPPAARRLERHPTDKVLGGVA
                     GGLGRYFGVDPIVFRLAFVGFALFGGGGILLYLIAWAAMPLGEGGEAGPARDGVDFAA
                     WVGLG"
BASE COUNT          346 a          976 c          965 g          312 t
ORIGIN      
        1 gccgcgcagc tccagctcgc tggcacgggc gggcgcccgc tgcccggcct cgggcacggc
       61 ctccggcggc gcggcaccgg cgccggccgc ctccagcagt tcctcgcgcg cgtccagcac
      121 gccgcgcacg cgctcccagc cggccacgga gcgcggcagg tcgccgagca ggaagccgag
      181 cacgcgcagc gggaacgcca gcaggctgaa caggtaggcg aactgcacca cgtcgcccgg
      241 cgtggccgcc cccgtgctga cgcgccagcc gcccagcagc agcacggcca ggatgccgag
      301 ctccgggagc acctccatgc tgggatcgaa cgtcgcgcgc aggcgcccca tgcggaccag
      361 ctcgtcgcgc aggcgctccg aggactgccg gaagcgctcc gtctcggcgg cctcgcggcc
      421 cagcgtcttc accaccagcg cgccgtcgaa gctttcgtgc gcgacctcgg agacctcgcc
      481 gcgcagctgc tgggcgcgga tcgccacacc gccgacgcgg cggttgtagg accagttcag
      541 cgcggtcatc accgcgccca tcgccaggcc gatgacggcc agcagcgggt cggtgagcac
      601 catcacggcc gcggcgatcg cgagcatgag gaccacgccc gcggaaaacg gcagcggcgc
      661 gatcgccccg aaggcggcct ccacgtcggc gtgggcggtg ctcagcagct cgcccgtgga
      721 gtggcggcgg tgccacgaca gcggcaggcg caggtagcgc gaggacacct cgcggcggtg
      781 ccgcgcctcc aggcggaact gcatgagggt cgccgcggtg cgccgcagca ccacaccgac
      841 gcccttcagc acgatcacgg cgaagatcgt cagcgccccc gccgccagcg cgccggtgcg
      901 gacgcgcccg gcctccaggg acggcagcac cacgtggtcg gtgacgcggc ccacgacgac
      961 ggcggagccg acggtcgtcg ccgcgtacag ggcggcgccc gccacggcgg cggcgaacga
     1021 gcggggctcc atgcggatgt acgcccacag caccgccatg ccgcggcgca gcacgctgcg
     1081 atgggtcgac ggggacacgg gaggcggcct cgatgggtcg gcggggtcag cccaccgagg
     1141 ctagcgagtc ggccgcgcgc ccgccggtta ggctccaggg ccgttgtccc taccgaacgg
     1201 gttggagcgg gtcatgacac tggcgctgag ccttccgatc accggcatgc cgctggacga
     1261 gaccgtcgcc tacgtgcgcg actgcgcgga gctgggctac cgcgccgtgt ggggcagcga
     1321 ggtcgccggt cccgacttcg cgaccctgct gggcgccgtg gccggcgccg gcctggacgt
     1381 cgacctcggc gtggcggtca tcccggtgca gacgcgcagc ccgtggctca tcgccgcgac
     1441 cgccgcgtcg ctggcgcaga tgagcggcgg gcgcttcagc ctggggctgg gcacgtccag
     1501 cgagctgatc gccgggcagt ggtccggcgc cccgttcgac aagccgctca cgcacctgcg
     1561 ggagacggtc gagacggtcc gggcgatcct ggccggcgag cgcgccaacg tcgacggcga
     1621 gtacgtgcgc acgcacggct acaagctgtt cgcgcccacg ccgccggtgc cgatcatcgt
     1681 gggcgcgctc aacgcggcga gcctgcgcca ggcggggcgc atcggcgacg gcgtggcgct
     1741 caaccagtcg gcgccggagc acctgccgat gatcctgggc gaggtgcgca agggcgccga
     1801 ggaggccggc aaggacttcg cggccatgcc ggtggtctcc cgcatcttct gcatggtcac
     1861 cgaccagccc gccgcgggcc gcgagatggt gcgccacatc ttcgcgccgt acgccgccac
     1921 gtcggtctac aaccgcttct tccgctggct gggcttcacc gaggagatgg aggccgtcgc
     1981 cgaggcgttc gggcgcaagg accgcgccgc ggcggccgcc gcgctgtccg accgctttgt
     2041 ggagtcgatc tacgtcatcg gcgatgccga ccacgtggcc cagcgcgtga aggactacgt
     2101 cgacaacggc gtcaccgtgg cggcgatcca gccgttggcc gccggccgcg acgacgcctt
     2161 gcgcaccatg aaggcgatcg ccgaggcgta cggcctgtag cgccggggcc ggtcgtccgg
     2221 cgttctcagg gtcttccccg atggcgcgcc tggcggccgc gtctcatcct gggtgccatg
     2281 aacgagacga gcgcaccccc cgccgacgcc atcccgccgc cggacgagcc acccccgccg
     2341 cagaccggcc cgccggcggc ccggcgcctg gagcgccatc ccaccgacaa ggtcctcggt
     2401 ggtgtggcgg gcgggctcgg ccgctacttc ggtgtcgacc cgatcgtgtt ccggctggcc
     2461 ttcgtcgggt tcgccctgtt cggcggcggc gggatcctgc tgtacctgat cgcgtgggcg
     2521 gccatgccgc tgggcgaagg cggcgaggcc ggaccggcgc gcgacggcgt cgacttcgcc
     2581 gcctgggtcg gcctgggcc
//
LOCUS       DATMKC010000311         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_217938, whole genome shotgun sequence.
ACCESSION   DATMKC010000311 DATMKC010000000
VERSION     DATMKC010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217938"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /gene="mreD"
                     /locus_tag="VNU01_09185"
     CDS             <1..478
                     /gene="mreD"
                     /locus_tag="VNU01_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWH32829.1"
                     /translation="FGHRPDLLVLFVVAVAYHDGPASGARAGFAAGLIGDLLSSASGP
                     VGVGALVLLLVGHAVGVLRPYLSGTALAGQLAVATGATLVAVLGYGFLGLLLDVAPAD
                     PALALQTAATVALYNALLAPFVFAAVRRLSVRFPRSTTVPASPWSPGRVSGQQGRP"
     gene            510..>2379
                     /gene="mrdA"
                     /locus_tag="VNU01_09190"
     CDS             510..>2379
                     /gene="mrdA"
                     /locus_tag="VNU01_09190"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWH32830.1"
                     /translation="MRTPQSPAGTDQAESTRLRLTFLTLLVVSLFILLFARLWFLQVM
                     AGERYSEMAQGNAIRTISIDAPRGKILDRNGRPVVRNRYANVISVKPAEMGEREAEVM
                     ADVAGLLGITVDELYERVRRSRVSPFRPKPVAVDVPGDIRDYIHENAATRFPGVYAET
                     RPLREYPNGSLAAHVVGYLGEISAEDLEDARYEGYRAGEIIGRTGVEQVHQQALRGVE
                     GQRRYVVNARGRVIGSLEELLPKPGADVQLTLDLEAQRFTEEALARGIEVARKVRDVA
                     TGPGRGGTFKAPAGAALVLDPRNGEVLALASFPTYEPSQFVGRLSPEYWAWLKAPENH
                     QPFLNRAVQSAYPPGSVFKPVSTAAALEAGYISTTTRLPCPRRWEWNGNVFRNWRGRD
                     MPSMNLAEALVDSCDTVYYELARRMWTDEVRAIEAGEEAYEHLSEQARGWGFGAPHGI
                     DLPSEAKGRVPGRRWKQEYWDAHKDTYCTQARQHPEGSYPQRLFTDLCNDGNRWRGGD
                     AVNMSIGQGDLLTTPLQVANAFAAIANRGTLWKPHVTKAVLHPDGRVDETQPEKLGEL
                     PVRREYLDYIDSGLQGVMAEGGTAGSVFADFPVRLAGKTGTAEMKPKQPFAWFAAYG"
BASE COUNT          321 a          879 c          862 g          317 t
ORIGIN      
        1 gttcgggcac cggcccgacc tgctggtcct gttcgtggtc gccgtggcct atcacgacgg
       61 ccccgcctcc ggcgcgcgcg cgggcttcgc cgccgggctc atcggcgacc tgctgagcag
      121 cgcgtcaggg ccggtcggcg tgggcgctct ggtcctgctg ctggtcggcc acgccgtcgg
      181 cgtgctgcgc ccgtacctgt ccggcaccgc ccttgccggc cagctcgcgg tcgccaccgg
      241 cgccacgctg gtcgccgtgc tgggctacgg cttcctcggg ctgctgctcg acgtggcgcc
      301 cgccgacccc gccctggcgc tgcagacggc cgccaccgtg gcgctgtaca acgcgctgct
      361 cgccccgttc gtgttcgcgg cggtgcgccg gctgtcggtg aggttcccgc gcagcaccac
      421 cgtgccggcc tcaccctggt cacccggacg ggtgtccgga cagcagggcc gcccctgacg
      481 gccgcgtgcg cccggctatc ctcggactcg tgcgcacccc gcagtcgccg gccggcacgg
      541 accaggccga aagcacgcgt ctccggctga ccttcctcac cttgctggtg gtcagcctgt
      601 tcatcctgct cttcgcgagg ctgtggttcc tgcaggtcat ggcgggggag cgctactcgg
      661 agatggccca gggcaacgcc atccgcacga tctccatcga cgcgccgcgc ggcaagatcc
      721 tggatcgcaa cggccggccg gtcgtgcgca accgctacgc gaacgtcatc tccgtcaagc
      781 cggcggagat gggcgagcgc gaggccgagg tcatggccga cgtcgccggg ctgctcggca
      841 tcaccgtcga cgagctctac gagcgcgtgc ggcgctcgcg cgtgagcccg ttccggccca
      901 agcccgtcgc cgtggacgtg cccggcgaca tccgcgacta catccacgag aacgcggcca
      961 cccggttccc cggcgtctac gccgagaccc ggccgctgcg cgagtacccc aacggctcgc
     1021 tggcggcgca cgtggtcggc tacctgggcg agatcagtgc ggaggatctc gaggacgcgc
     1081 gctacgaggg ctaccgggcc ggcgagatca tcgggcgcac cggcgtggag caggtccacc
     1141 agcaggcgct gcgcggcgtc gagggccagc ggcgctacgt ggtcaacgcc cgcggccgcg
     1201 tgatcgggtc gctggaggag ctgctgccca agccgggcgc cgacgtgcag ctcacgctgg
     1261 acctggaggc gcagcgcttc accgaggagg ccctggcgcg cggcatcgag gtggcgcgca
     1321 aggtgcgcga cgtcgcgacc ggcccgggcc gcggcggcac gttcaaggcg cccgccgggg
     1381 cggcgctggt gctggacccg cgcaacggcg aggtcctcgc gctggccagc ttcccgacct
     1441 atgagccctc gcagttcgtc ggccggctca gcccggagta ctgggcgtgg ctgaaggccc
     1501 ccgagaacca ccagcccttc ctgaaccggg ccgtgcagtc ggcctatccg cccggctcgg
     1561 tgttcaagcc ggtgtccacg gccgccgccc tggaggccgg ctacatcagc accacgacgc
     1621 ggctgccgtg cccgcggcgc tgggagtgga acggcaacgt gttccgcaac tggcgcgggc
     1681 gcgacatgcc gagcatgaac ctggcggagg ccctggtcga ctcctgcgac accgtgtact
     1741 acgagctggc gcggcgcatg tggaccgacg aggtgcgcgc catcgaggcc ggggaagagg
     1801 cctacgagca cctcagcgag caggcgcgcg gctggggctt cggcgccccc cacggcatcg
     1861 acctgccgtc ggaggcgaag ggccgtgtgc cggggcggcg ctggaagcag gagtactggg
     1921 acgcgcacaa ggacacctac tgcacccagg ctcgccagca cccggagggc agctacccgc
     1981 agcggctgtt caccgacctg tgcaacgacg gcaaccgctg gcgcggcggt gacgcggtca
     2041 acatgtccat cgggcagggc gacctgctca ccacgccgct gcaggtggcc aacgcgttcg
     2101 ccgcgatcgc caaccgcggc acgctgtgga agccccacgt caccaaggcg gtgctgcacc
     2161 ccgacggccg cgtggacgag acccagccgg agaagctcgg cgagctgccg gtgcgccgcg
     2221 agtacctcga ctacatcgac agcggcctgc agggcgtcat ggccgagggc ggcacggccg
     2281 gcagcgtgtt cgcggacttc ccggtgcggc tggcgggcaa gaccggcacc gcggagatga
     2341 agcccaagca gccgttcgcc tggttcgccg cctacgggc
//
LOCUS       DATMKC010000312         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_146068, whole genome shotgun sequence.
ACCESSION   DATMKC010000312 DATMKC010000000
VERSION     DATMKC010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146068"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VNU01_09195"
     CDS             <1..1146
                     /locus_tag="VNU01_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HWH32831.1"
                     /translation="GHGGPRHPGGPDDGPVQRGLLEEPVIVRQLPARAGDARGFFRKS
                     RDVVMGTERRIGLWVLPLFLVMGLLGATLAGGLAMLYYGQQVRDLERSTTRVVSQLEE
                     RGAELERIAEEARTSIEDQVRQVRETIAQEAPITSPNEAGIYAVAAAHPGGEVRVGSG
                     FTIFSDASETFLVTSYALVATPDGFAVRTAEVYLPSDTVTASVHNYDRDLDIATLRLR
                     GGPLPVPEWRPGEQEVRRGDLVYAAGIAGPGTPTVVEGTIAGVSAEAIVPGLSLNAFL
                     AGGPLVDASGKVIAIASISYRPFGPVDGDLHYAVPIRSVCRRLVRCTRADLSAGELGD
                     EGGSGTIRTTPPPEPPPSPSPTQEERRPSPSPTASPPPTAPATTPPP"
     gene            complement(1143..3266)
                     /locus_tag="VNU01_09200"
     CDS             complement(1143..3266)
                     /locus_tag="VNU01_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWH32832.1"
                     /translation="MSRKRLAARIAVTLVAFAVASGLMVAAFGMIALAIDWEIPERNE
                     LLGPSIAVDRHGRPLARFAADVERRPVPIDQISEHLQDAVVAKEDHRFYDHEGVDPIS
                     VLRAVWRNVTTGGIAEGGSTLTQQYVKMVYVGRERTLYRKIREAVVALQLDKERSKEE
                     ILEAYLNRAYFGDGAYGAQAAALTYFGKPAADLALHEAALLASVLSAPTRLSPRVDPD
                     GALFRRNQVLDIMAANGFAVRERVREAKLQPLGVVPYQREAPKAPYFVDELRRQLLDA
                     FGPEAVYNGGLQVTATIDLDQQALLEQQVAPHLPPNPEIDAGVASLDPRTGDVLAAWG
                     GRDYSVSQVNLALNQDYGRPSGSTFKPIVLATALEEGMTLNRRYPAPRQVRIGDWTPS
                     GGGCGGSCTLLQATARSVNTVFAQLGRDVGVEDFVQMAHRLGVRSSLLQRDGGPPDIT
                     RSLGTGNVTPLDLASAFGTFANNGVACPARLVLEVRAPDGTPLPAPDPRQPSAEERKA
                     WAARLHDMGYEFDDDDLGRCYRAVAPSVARDVTRALEAAVDSGTGTRAKIGRPQAGKT
                     GTHQFNTEIWFVGYTPQFSMAVHLAHRDRQVPLEGLPGCGRECFGGTIPAMIWRDAAS
                     AMLADVPPEPFPEPGPDERVYPSRRRLGPAMRSPEPRPEPEPTTPSPEPTESETGEPT
                     GSPSPGPGDGVLPFPPVAGDPQGDG"
     gene            complement(3340..>3775)
                     /locus_tag="VNU01_09205"
     CDS             complement(3340..>3775)
                     /locus_tag="VNU01_09205"
                     /inference="COORDINATES: protein motif:HMM:NF020772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HWH32833.1"
                     /translation="VRSLAGGPVRPTTLRAYLDRHPPRRRLALPESSWGWGKGHAAWV
                     SERTRWIWQALRGAEAEFRALPATADWAGARDAAWRQLSLLQASDWPFMINRDQSAQY
                     AEERVRAHLERFRQACRGEGLDELAARDDPGGTRAPRPAPVA"
BASE COUNT          443 a         1397 c         1453 g          482 t
ORIGIN      
        1 ggccacggcg gtccccggca ccccggcgga ccggacgacg gcccggtcca gcgcggcctg
       61 ctggaggagc ccgtcatcgt ccggcagctg ccggcgcgcg ccggggacgc gcgcggcttc
      121 ttccgcaaga gccgcgacgt cgtcatgggc accgagcggc gcatcgggct gtgggtgctg
      181 ccgctgttcc tggtgatggg gctgctgggc gccacgctgg ctggcggcct ggcgatgctg
      241 tactacggcc agcaggtgcg cgacctggag cgctccacca ccagggtcgt gtcccagctc
      301 gaggagcgcg gcgcggagct ggagcgcatc gccgaggagg cgcgcacgtc gatcgaggac
      361 caggtccgcc aggtgcgcga gaccatcgcg caggaggcgc cgatcacctc gcccaacgag
      421 gcgggcatct acgcggtggc cgccgcccac cccggcggcg aggtgcgggt gggcagcggc
      481 ttcaccatct tcagcgacgc cagcgagacg ttcctggtga cctcctacgc gctggtggcc
      541 accccggacg gcttcgcggt gcgcaccgcc gaggtctacc tgcccagcga cacggtgacc
      601 gcctcggtgc acaactacga ccgcgacctg gacatcgcga cgctgcgcct gcgcggtggc
      661 ccgctgccgg tgccggagtg gcggcccggc gagcaggagg tccgccgcgg cgacctggtc
      721 tacgccgccg gcatcgcggg gccgggcacg cccaccgtgg tggagggcac gatcgccggc
      781 gtgagcgcgg aggcgatcgt cccggggctg tcgctcaacg cgttcctggc cggcgggccg
      841 ctggtcgacg cctccggcaa ggtcatcgcc atcgcctcga tcagctaccg gccgttcggg
      901 ccggtggacg gcgacctgca ctacgcggtg ccgatccggt cggtgtgccg gcgcctggtg
      961 cgctgcacgc gcgcggacct gtcggccggt gagctgggcg acgagggcgg cagcggcacc
     1021 atccgcacca cgccgccgcc ggagccgccg ccctcgccgt cgccgacgca ggaggagcgc
     1081 cggccgtcgc catcgccgac cgcgtcgccg ccgccgaccg ctccggcgac cacgccgcct
     1141 ccctaaccgt ctccctgggg gtctccggcg accggcggga acggcagcac gccgtcgccg
     1201 ggacccggtg acggcgagcc cgtgggctcg cccgtctcgg actccgtcgg ctcgggcgac
     1261 ggcgtcgtcg gctccggctc cgggcgcggc tcgggggagc gcatcgcggg gcccaggcgc
     1321 cgccggctcg ggtacacgcg ctcgtcgggg ccgggctccg ggaacggctc cggcggcacg
     1381 tccgccagca tcgccgaggc ggcgtcgcgc cagatcatcg cggggatcgt gccgccgaag
     1441 cactcgcggc cgcagcccgg cagcccttcc agcggcacct ggcggtcgcg gtgggccagg
     1501 tgcacggcca tggagaactg cggggtgtag cccacgaacc agatctcggt gttgaactgg
     1561 tgcgtgccgg tcttgccggc ctgcgggcgc ccgatcttgg cgcgcgtgcc cgtgccgctg
     1621 tccaccgccg cctccagcgc gcgggtcacg tcgcgcgcca cggacggggc gacggcgcgg
     1681 tagcagcggc cgaggtcgtc gtcgtcgaac tcgtagccca tgtcgtgcag gcgggccgcc
     1741 catgccttgc gctcctccgc gctgggctgg cgcgggtccg gcgccggcag cggcgtgccg
     1801 tcgggcgcgc gcacctccag caccagccgc gccgggcacg ccaccccgtt gttggcgaac
     1861 gtgccgaagg cggaggcgag gtccagcggg gtgacgttgc ccgtgccgag cgagcgggtg
     1921 atgtccggcg gcccgccgtc gcgctgcagc aggctcgagc gcacgcccag ccggtgcgcc
     1981 atctgcacga agtcctccac cccgacgtcg cggcccagct gcgcgaacac ggtgttgacg
     2041 gagcgcgccg tggcctgcag cagcgtgcag ctgccgccgc agccgccgcc gctgggcgtc
     2101 cagtcgccga tgcgcacctg gcgcggcgcc gggtagcggc ggttgagggt catgccctcc
     2161 tccagcgcgg tcgccaggac gatgggcttg aacgtcgagc cggacgggcg gccgtagtcc
     2221 tggttcagcg ccaggttgac ctggctcacc gagtagtcgc ggccgcccca ggcggccagc
     2281 acgtcgcccg tgcgcgggtc gagcgaggcc acgcccgcgt cgatctccgg gttgggcggc
     2341 aggtgcgggg ccacctgctg ctccagcagc gcctgctggt ccaggtcgat ggtcgcggtg
     2401 acctgcaggc cgccgttgta gaccgcctcg gggccgaagg cgtccagcag ctggcggcgc
     2461 agctcgtcga cgaagtacgg ggccttgggc gcctcccgct ggtagggcac cacgcccagg
     2521 ggctggagct tggcctcgcg gacacgctcg cgcacggcga agccgttggc ggccatgatg
     2581 tccagcacct ggttgcggcg gaacagcgcg ccgtccgggt cgacgcgcgg cgacagccgc
     2641 gtgggggcgg acagcacgct ggcgagcagg gcggcctcgt gcagggccag gtccgcggcg
     2701 ggcttgccga agtaggtcag ggcagcggcc tgcgcgccgt aggcgccgtc gccgaagtac
     2761 gcgcggttga ggtaggcctc gaggatctcc tccttggagc gctccttgtc cagctggagg
     2821 gcgaccaccg cctcgcggat cttgcggtac agcgtgcgct cgcggccgac gtagaccatc
     2881 ttcacgtact gctgggtcag cgtggagccg ccctcggcga tgccccccgt ggtgacgttg
     2941 cgccacaccg cgcgcagcac ggagatcggg tccacgccct cgtggtcgta gaagcggtgg
     3001 tcctccttgg ccacgaccgc gtcctgcagg tgctcgctga tctggtcgat gggcaccggc
     3061 cggcgctcca cgtcggcggc gaagcgggcc agcgggcggc cgtggcggtc cacggcgatg
     3121 gacggtccca ggagctcgtt gcgctcggga atctcccagt cgatggccag cgcgatcatg
     3181 ccgaaggcgg ccaccatcag cccgctcgcg accgcgaagg cgacgagcgt gacggcgatg
     3241 cgggcggcca gccgcttgcg ggacatgccc cgagtctacg gacgttgcgc cggagcgggc
     3301 ctggaacggc cctgttcgcc cgggcggtgg cgcggccggt caggcgaccg gggcgggcct
     3361 gggcgcgcgc gtgccgcccg ggtcgtcgcg ggcggccagc tcgtccagcc cctcgccacg
     3421 gcatgcctgg cggaagcgct ccaggtgcgc gcgcacccgc tcctcggcgt actgcgccga
     3481 ctggtcgcga ttgatcatga acggccagtc gctggcctgc agcaggctga gctggcgcca
     3541 ggcggcgtcg cgcgcgccgg cccagtccgc cgtcgccggc agcgcccgga actcggcctc
     3601 ggccccgcgc agcgcctgcc agatccagcg cgtccgctcg ctgacccagg ccgcgtgccc
     3661 cttgccccag ccccaggacg actcgggcag ggccagccgc cgccgcggcg ggtgtctgtc
     3721 caggtaggcg cgcagggtgg tgggccgcac gggcccgccg gcgagcgagc gcacc
//
LOCUS       DATMKC010000313         4316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_289258, whole genome shotgun sequence.
ACCESSION   DATMKC010000313 DATMKC010000000
VERSION     DATMKC010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289258"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VNU01_09210"
     CDS             complement(<1..260)
                     /locus_tag="VNU01_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32834.1"
                     /translation="MNSAATGCDDAAPRGVMPTMSSRRVESLLGPLIVLLLVGGVTAA
                     VLGGGDDETEFAEQPTEFADPFDDPSGDDEPGGQSPAPAEEPT"
     gene            314..979
                     /locus_tag="VNU01_09215"
     CDS             314..979
                     /locus_tag="VNU01_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWH32835.1"
                     /translation="MTTSSPAATAGRPLLRGWLHAAMTPVAVGAGLWLLRGASAPANR
                     LSVAVFAACMVGLYGTSAAYHVGRWGERARYILSRCDVAMIQLAIAGTFTPLAFHTLE
                     GGWRTWSLVVAWGVALIGAAIAASPITGPRWLTAAGYVATGWLAVVPLSRMVQALPWE
                     GTGLIVLGGLIYMLGAVVYVRQRPNPLPAWFGFHEVFHLLVIAASCCHYYAIWRYVLP
                     LGA"
     gene            complement(1213..2475)
                     /locus_tag="VNU01_09220"
     CDS             complement(1213..2475)
                     /locus_tag="VNU01_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32836.1"
                     /translation="MPEDIEQAWDGFLAQPGVALAIGGMLDTSELWLHRRVERLTLHS
                     ATVCNRHVSLDLSVPEDAPPLPWNSRQLLLPLTFLQKQPLTRFDLHDSSGGPVPVLTA
                     GQNGELALTILSVMGEAVAGKELSRETQDQLSAVVAASGSPGSLDRVWDAFVDRVVDG
                     PDAALFDHDGYLKLVEAFLEQFVLVAVLSEGPGVRTVLKFSYDTAFTLTEGENRLRRF
                     RTRAGLRPAEVSFDVVSLLPASSTHVEVQEPEDVEVLATGALREAAEPRAGAGVAGPR
                     RHLHLGRLGAGEAVQVNLQVVPARTGWMQGSRWSAWLAAFLLFGVYLRAPVLFHRGAD
                     DSASLLLAVTAVVVGLVTRPPAHAIAARLVAVRVRLVWLPPVALMLVAAVLVLEMQQT
                     VGRALVGICAVAVSAVAVLLSLRPVRAH"
     gene            complement(2533..3450)
                     /gene="sucD"
                     /locus_tag="VNU01_09225"
     CDS             complement(2533..3450)
                     /gene="sucD"
                     /locus_tag="VNU01_09225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWH32837.1"
                     /translation="MSILIDQHTRLVVQGTGSQGTFHAKRNQGYGTKVVAGVHPKKGG
                     TTWEGLDVPIRASVAEAVESDGANTSMIMVPAPGAKDAILEAYEAGISTIVCITEGVP
                     VHDMAVVYNTLYPRQADGTFLKEGRPVLIGPNCPGLISPGKANVGIIPAEITKPGKVG
                     LVSRSGTLTYQIMHELNLAGIGISTCVGIGGDPIIGSNFLDVIPRFAEDPETEAIVFV
                     GEIGGQEEQKAGDWIKANIPDTPVVAYIAGFSAPPGKQMGHAGAIVKEGGGGGETAAD
                     KKAYLESLGISVGTNPTETAELMIQRLNA"
     gene            complement(3464..>4316)
                     /gene="sucC"
                     /locus_tag="VNU01_09230"
     CDS             complement(3464..>4316)
                     /gene="sucC"
                     /locus_tag="VNU01_09230"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWH32838.1"
                     /translation="YYLSILHDRVSKGYLVICSVEGGMDIEEVNRTRPEAVVKEPIDP
                     IAGIDRAKAEQILRAARIPDAVLDQAADLLVKLYEGFVDSDATLFEVNPLIRTEDGRV
                     IALDSKVSIDGNALFRHADLAEWENIEAADPLEAEAKRQDLAYVKLDGDVGIMGNGAG
                     LVMSTLDVVTQAGGKPANFLDAGGGASAESMAKGIAFVLGDPSVKAMLINIFGGITRC
                     DDVAKAVLGALDQLGDVEQRLVVRLDGTNADEGRRILAEADNPKIVAASKMNEAAQKA
                     VELAAQA"
BASE COUNT          647 a         1525 c         1440 g          704 t
ORIGIN      
        1 gtgggctcct ccgccggagc gggcgactgg cccccgggct cgtcgtcgcc ggacgggtcg
       61 tcgaacgggt cggcgaactc ggtgggctgc tccgcgaact ccgtctcgtc gtcgccgcct
      121 cccagcacgg cggccgtcac gccgccgacg agcagcagca cgatgagcgg gcccagcagg
      181 gattcgacgc gtcgggacga catggtcggc atcacaccac gcggcgccgc gtcgtcacaa
      241 cccgtggcgg cggaattcac gcgcccaggg tgggcgtttt gcccggagcg gccgcaggcg
      301 tcactcttgg gccgtgacca cctcgtcccc cgccgcgacc gccggccgcc cgctcctgcg
      361 cggatggctg cacgccgcga tgacaccggt cgccgtggga gcgggcctgt ggctgctgcg
      421 cggcgcgagc gccccggcca accgcctgtc cgtcgcggtg ttcgcggcgt gcatggtggg
      481 gctgtacggg acctcggccg cctatcacgt gggccgctgg ggcgagcggg cgcgctacat
      541 cctgtcccgc tgcgacgtcg cgatgatcca gctggccatc gccggcacgt tcacgccgct
      601 ggcgttccac acgctggagg gcggctggcg cacctggagc ctggtggtgg cctggggcgt
      661 ggcgctgatc ggcgcggcga tcgccgcctc gcccatcacg gggccgcgat ggctgaccgc
      721 ggcgggctac gtcgcgaccg gctggctcgc cgtggtgccg ctgtcgcgca tggtgcaggc
      781 gctgccgtgg gagggcacgg gcctgatcgt gctcggcggg ctgatctaca tgctgggcgc
      841 cgtggtctac gtgcggcagc ggccgaaccc cctgcccgcc tggttcggct tccacgaggt
      901 gttccacctg ctggtgatcg ccgcgagctg ctgccactac tacgcgatct ggcgctacgt
      961 cctgcccctg ggcgcctgag ggacctggac ctaccggtca acccttgctg tagcgcttct
     1021 tggcggaggc gaccagggct aggcgcgagg cgcgcatctc cggagtccag acaatggagc
     1081 gcgtaagagg cgcctcagcg gaatcgacgt gaaaacgtcc ggccttcggt tgtgctcgta
     1141 ccactttccc gttgttggcc atcgccgacc tccctttgtg atcacttagc atgatgccca
     1201 agcggtgcga catcaatgag cccgcaccgg tctcagtgag agcaacacgg ccacagcgct
     1261 gacagcaacg gcgcaaattc cgaccagagc cctgcccacc gtctgctgca tttcgagtac
     1321 caggacggct gccacgagca tcaacgcgac cggaggcagc cacaccagtc ttacgcgaac
     1381 ggccacgagg cgagccgcga tggcatgcgc gggaggccgg gtgacgagac cgaccacgac
     1441 cgcagtcacg gccaacagaa gcgacgctga gtcatcggca cctctgtgga acaacacggg
     1501 agcgcgaagg taaaccccga acagaaggaa cgcagccaac caggccgacc acctcgatcc
     1561 ttgcatccac cctgtccttg cgggaacgac ctggaggttg acctggaccg cctccccagc
     1621 ccccagacgc cccaggtgaa ggtgtcgccg tggcccggcc actcctgcgc ccgcacgggg
     1681 ctccgcggcc tcccgtagcg cccctgtcgc cagaacttcc acgtcttcgg gctcctggac
     1741 ctccacatga gtgctcgaag ctggcaagag agacaccacg tcaaacgaca cctccgccgg
     1801 acgaaggcct gcccgcgtcc tgaagcgacg gagccggttt tcgccctccg tgagggtgaa
     1861 cgctgtgtcg taggagaact tcagcacggt acgaacccct ggaccctcac tcagcacggc
     1921 cacgaggacg aactgttcga ggaaggcctc tacgagcttc aggtacccat cgtggtcgaa
     1981 gagtgcagcg tctggaccgt ctacgactcg gtccacgaac gcgtcccaga cgcgatccaa
     2041 gctacccgga gacccgctgg cagcgaccac agcgctgagc tgatcctgcg tctcacgtga
     2101 caactccttg cctgcgaccg cctcgcccat cactgacagg atcgtgaggg ccaattcacc
     2161 attctgtccc gctgtcagca cagggactgg accgccgctc gagtcgtgca ggtcgaaccg
     2221 ggtcaggggc tgcttctgta gaaaggtcag cggaaggagc aactgccgac tgttccaagg
     2281 cagtggcggc gcgtcttcgg gcacgctcag atctaggctc acgtgccggt tgcaaaccgt
     2341 ggccgagtga agggtcaacc gttccacccg gcgatggagc cacaactcac tggtgtccag
     2401 catgccgccg atcgcaagcg ccacgccggg ctgggccagg aacccatccc acgcctgctc
     2461 gatgtcctca ggcacgactc acccccgatt ccgccgggga aggttatcgg gcgagtgaga
     2521 caggaccgga cttcaggcgt tgaggcgctg gatcatgagc tcggcggtct cggtcgggtt
     2581 ggtgccgacg gagatgccca gcgactccag gtaggccttc ttgtcggccg cggtctcgcc
     2641 gccgccgccg ccctccttga cgatggcgcc ggcgtggccc atctgcttgc ccggaggcgc
     2701 ggagaacccg gcgatgtagg cgacgacggg cgtgtcgggg atgttcgcct tgatccagtc
     2761 gccggccttc tgctcctcct ggccgccgat ctcgcccacg aacacgatcg cctcggtctc
     2821 ggggtcctcg gcgaagcgcg ggatgacgtc caagaagttg gagccgatga tgggatcgcc
     2881 gccgatgccg acgcaggtcg agatgccgat cccggccagg ttcagctcgt gcatgatctg
     2941 gtaggtcagc gtgccggagc ggctcaccag gccgaccttg ccgggcttgg tgatctccgc
     3001 ggggatgatc ccgacgttgg ccttgccggg cgagatcagc cccgggcagt tcgggccgat
     3061 cagcaccggg cgcccctcct tgaggaacgt cccgtccgcc tggcgcgggt agagcgtgtt
     3121 gtagaccacg gccatgtcgt gcacgggcac gccctcggtg atgcacacga tcgtggagat
     3181 gcccgcctcg taggcctcca ggatcgcgtc cttcgcgccc ggcgccggga ccatgatcat
     3241 cgacgtgttc gcgccgtcgg actccacggc ctccgccacc gacgcgcgga tcggcacgtc
     3301 caggccctcc cacgtcgtgc cgcccttctt cgggtgcacg ccggcgacga ccttggtgcc
     3361 gtagccctgg ttgcgcttgg cgtggaacgt gccctgggag ccggtgccct ggaccacgag
     3421 ccgggtgtgc tgatcgatca ggatgctcat ccgtggactc cctttaggcc tgcgcggcca
     3481 gctcgacggc cttctgcgcg gcctcgttca tcttcgacgc ggcgacgatc ttcgggttgt
     3541 ccgcctcggc caggatgcgc cggccctcgt cggcgttcgt gccgtccagg cgcacgacca
     3601 gccgctgctc cacgtcgccg agctggtcca gcgcgccgag caccgccttg gcgacgtcgt
     3661 cgcagcgcgt gatgccgccg aagatgttga tcagcatggc cttgaccgac gggtcgccca
     3721 ggacgaacgc gatgcccttg gccatgctct cggcgctggc gccgccgccg gcgtccagga
     3781 agttcgccgg cttgccgccc gcctgcgtga ccacgtccag cgtggacatc accaggccgg
     3841 cgccgttgcc catgatcccc acgtcgccgt cgagcttgac gtaggcgagg tcctggcgct
     3901 tggcctccgc ctccagcggg tcggcggcct cgatgttctc ccactcggcc aggtcggcgt
     3961 ggcggaacag cgcgttgccg tcgatggaca ccttggagtc cagggcgatg acccggccgt
     4021 cctcggtgcg gatcagcggg ttcacctcga acagcgtcgc gtcggagtcc acgaagccct
     4081 cgtacagctt caccagcagg tccgcggcct ggtccagcac cgcgtcgggg atgcgcgccg
     4141 cccgcaggat ctgttccgcc ttggcgcggt cgatgccggc gatcgggtcg atgggctcct
     4201 tgaccaccgc ctccggccgc gtgcggttga cctcctcgat gtccatgccg ccctcgaccg
     4261 agcagatcac caggtagccc ttgctgaccc ggtcgtgcag gatcgacagg tagtac
//
LOCUS       DATMKC010000314         6692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_151919, whole genome shotgun sequence.
ACCESSION   DATMKC010000314 DATMKC010000000
VERSION     DATMKC010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151919"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VNU01_09235"
     CDS             <1..678
                     /locus_tag="VNU01_09235"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HWH32839.1"
                     /translation="CHDRFVRLVHGAGSRGEDRWRELREATEFAYVLGGGGGLEAYAL
                     VRHQADPDDWRYVLAVEDWAALTPPGLEAVLGFVGRHGTLAKAAVLAGPAVRLLGGHL
                     PEQDWRLTTDFSWMARGLDFSAAIAARPFPAGIGAELTIALEDPLLPEIQGPWRVTVA
                     GGKGLAEPAGEAAVRLRPRAVGPLLMGSAGADTLILGGMADGTPEDLALLSGVFAAPP
                     PVFLDFF"
     gene            704..1432
                     /locus_tag="VNU01_09240"
     CDS             704..1432
                     /locus_tag="VNU01_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH32840.1"
                     /translation="MADHLDAVLDHVAIAVPAWEPAERRWRDDLGGGVVAFGKTPAFW
                     SRQLRFAGGGKLELLAPPPETGPDNFVRRFLDRFGAHLHHVTLKVPDLMEAVAVLRAA
                     GLEPVDIRADNPYWHEAFLRPSEAGGIVVQVAYAGQSDEEWARKVGLEPERPRPGAAT
                     LTGPLLEHPDLAAAERLWRLLGAEIARDDSGLMASWPRSPLTVRIARGPAARAVALLG
                     HGVPPQQSDPETGPELRFSGIPPA"
     gene            1642..2199
                     /locus_tag="VNU01_09245"
     CDS             1642..2199
                     /locus_tag="VNU01_09245"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HWH32841.1"
                     /translation="MRLQIAAMLAGMAMVSGLVFQTSSAAFSGTTASSGNTFTAGTVS
                     LSDDDAAAAMFSLSNLKPGDTSTKCIQLTYTGSLASAIKLYGAIGGGTGLGAYIDLTI
                     TSGTPGTFADCSTFAGTQIYSGTLANFATTHANWSNGLSDANWTPSTNGQTRTYRFVT
                     TLQDNNSAQGLSANTVSFTWEAQNT"
     gene            2257..3162
                     /locus_tag="VNU01_09250"
     CDS             2257..3162
                     /locus_tag="VNU01_09250"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWH32842.1"
                     /translation="MDARPETTRRGAADWLVLVGRFAALVYLNALVWLSVWVLIPTLF
                     MGWQPMAIETGSMGPSIRTGDVVLVESYRGQPLEAGRVITFRDPADPDRLVTHRITAV
                     NADGTYLTKGDANLDADSTPLAGEHIRGVGRLVIPRVGLPVVWMEQGHYAALGAWAVS
                     VVFALVLLAPSRPEEQPSRVPLSARVPRFPRRSAPAPVAPLPGPAVPVASLPVPPGGP
                     SRTLPVAAGWAQVPAVRYRLRLPVPPMALELPAPPRWTVALVPSPPGTAPVAPIPAPP
                     RPAAVRRGTALSSRDVPGWAGRLHS"
     gene            3235..3669
                     /locus_tag="VNU01_09255"
     CDS             3235..3669
                     /locus_tag="VNU01_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32843.1"
                     /translation="MRRRAWIRLLVVLPLLAALAVLDVSDAVFTGGTSNGGSSFEGDT
                     LAPPSGVTVTHQCLLSIQSVKVDWTASPSTWATHYRVLRAVNGGAYASVATVSYGTDT
                     WTDSGVSASTTYTYRVRTERTGWTWTSVEAQSADVTTPALCL"
     gene            3842..4720
                     /locus_tag="VNU01_09260"
     CDS             3842..4720
                     /locus_tag="VNU01_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32844.1"
                     /translation="MLLVTMLVLRVSGAAFQASTVNPSNSFGAATVAVSDNDSGTLLL
                     DTTAMLPGDTKQTCVQVSYTGTVDATVKLHGTVSGSLPQYLNLTVERGSGASAFGNCT
                     GFSSTATVFSGTVSGLASSHTSWADGAGSYTAAPSSSQWFRIVWTLQDVNAAQGQTAS
                     ATYTWEARSGAVTTNFRSTTASSTATVPADTLQPPGTPSATYNCTLTVRQITVSWTAT
                     PSTWATTYRVYRSVDGGAYSSVATVEYGTTSWVDTAVNGGTTYIYKLRSERTGWTWVS
                     ADTAVSNSVTTPTLCL"
     gene            complement(4724..5767)
                     /locus_tag="VNU01_09265"
     CDS             complement(4724..5767)
                     /locus_tag="VNU01_09265"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWH32845.1"
                     /translation="MASPSRRVAVVGATGAVGQDLRRLLEQRAFPCDEVVLVASPRSE
                     GKVLTFRGQDVTVRSIEDPAVFEGVDIALFSAGGGTSREHAPRAVEAGAIVVDNSSAW
                     RMDPDVPLVVSEVNPEALERIGKGIVANPNCTTMAIMLPLKALHDAFGLVDLVATSYQ
                     AAGGAGQKGIDELAAQVPVLHEHVDLLRADGAAAAKLVEHKVHAATLAYNVVPLLGTP
                     GEGGYTDEELKLQNESRKILGIPDLRVSPTCVRVPVMVGHSVQCRARFSRPVTREAAL
                     AAMDGFPGLVLDEVPTPLEWAGRDEVAVGRVREDLGDDHALNLFVVGDNLLKGAALNT
                     VQIAELLVARGLR"
     gene            complement(5760..>6692)
                     /locus_tag="VNU01_09270"
     CDS             complement(5760..>6692)
                     /locus_tag="VNU01_09270"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWH32846.1"
                     /translation="ITDSAHGKARIMDITPGRIHEALEEGHVVIVAGFQGVSRDSKDV
                     TTLGRGGSDTTAVALAGALGADVCEIYTDVNGVYTADPRIVPDARHLSRVSHEEMLEL
                     AAHGAKVLQVRSVEYARNHGVTVHVRSSFTYDEGTWVMSDSEMDEGMEAAMVTGVAHD
                     SGEGKLVIRGVPDKPGIAARIFTVLAANHINVDMIVQNVSESGATDISCTLPLADGPK
                     AVQILRELAAEVGAAGVDFDEGIAKVSLVGAGMKTHPGVAAAMFEALSEVGVNIEMIS
                     TSTIRISVVIRESDLEAAVRAVHDRFNLAEADHG"
BASE COUNT          911 a         2460 c         2345 g          976 t
ORIGIN      
        1 tgccacgacc gcttcgtccg gctggtgcac ggcgcgggca gccgcgggga ggaccgctgg
       61 cgcgagctgc gcgaggccac cgagttcgcc tacgtgctcg gcggcggcgg cggactggag
      121 gcctacgcgc tggtgcgcca ccaggccgac cccgacgact ggcgctacgt cctcgccgtc
      181 gaggactggg ccgcgctgac gccccccggg ctggaggccg tgctcggctt cgtcggccgg
      241 cacggcaccc tggcgaaggc ggccgtgctc gccggcccgg ccgtgcgcct gctgggcggg
      301 cacctgccgg agcaggactg gcggctgacc acggacttct cctggatggc gcgcgggctg
      361 gacttctccg cggccatcgc ggcgcggccc ttccccgcgg gcatcggcgc ggagctcacc
      421 atcgcgctgg aggacccgct cctgccggag atccagggtc cgtggcgcgt gacggtcgca
      481 ggcggcaagg gcctcgccga gccggcgggt gaggcggccg tgcgcctgcg cccgcgcgcg
      541 gtcggaccgc tgctgatggg ctcggccggg gccgacacgc tgatcctcgg cgggatggcg
      601 gacggcacgc ccgaggacct cgccctcctg agcggcgtgt tcgccgcccc gccgccggtg
      661 ttcctcgact tcttctgacc cgacccgcga ccggagagac gacatggccg accatctcga
      721 cgccgtcctg gaccacgtcg ccatcgccgt gcccgcctgg gagccggccg aacggcgctg
      781 gcgggacgac ctgggcggcg gcgtggtcgc gttcggcaag acgcctgcgt tctggagccg
      841 gcagctgcgc ttcgccggcg gcggcaagct ggagctgctc gcgccaccgc cggagaccgg
      901 tccggacaac ttcgtgcggc gcttcctgga ccgcttcggc gcccacctgc accacgtcac
      961 gctgaaggtg cccgacctta tggaggcggt cgcggtgctg cgggcggcgg ggctcgaacc
     1021 ggtcgacatc cgggcggaca acccgtactg gcacgaggcg ttcctgcggc ccagcgaggc
     1081 cggcgggatc gtggtgcagg tcgcctacgc cggccagagc gacgaggagt gggcccgcaa
     1141 ggtcgggctg gagcccgagc ggccgcgccc cggggcggcg accctgaccg gcccgctgct
     1201 ggagcacccc gacctggcgg ccgccgagcg cctctggcgc ctgctgggcg cagagatcgc
     1261 ccgcgacgac agcggactga tggcgtcgtg gccccgctcg ccgctcacgg tccgtattgc
     1321 ccggggtccc gcggcccgcg cggtggcact tctgggccac ggcgtgccgc cgcagcaatc
     1381 cgaccccgag accggccctg agctgcggtt ttccggcatc ccgccggcct gatcctcggg
     1441 cgtacgcaca tacgtagcca tttctacgta gtcacgcaca gccatttacg tagatgtgct
     1501 tacgttttcg ctcgctcgtg ccgagacaca agggtcggca cctgtacggg ccgggaacac
     1561 cgcgaggtga cacgccagtg aggggatgtc gcggaggtcc caggcgctcg tctatgtagg
     1621 gagcgagtga tgactggaac gttgcgactg cagatcgccg cgatgctggc gggcatggcg
     1681 atggtgagcg gcttggtctt ccagacctcg agcgccgcct tcagtggcac cacagccagc
     1741 tcgggcaaca cctttacggc gggcaccgtg tcgctgtcgg acgacgacgc cgcggccgcg
     1801 atgttctcgt tgtccaacct gaagccgggc gacacctcca cgaagtgcat ccagctgacc
     1861 tacacgggct cgctggccag cgcgatcaag ctgtacgggg ccataggcgg gggcaccggt
     1921 ctcggcgcgt acatcgacct gaccatcacg agtggcacgc caggcacctt cgcggactgc
     1981 agcacctttg cgggcaccca gatctattcc gggaccctgg cgaacttcgc caccacgcac
     2041 gcgaactgga gcaatggcct gtccgacgcc aactggacgc ccagcaccaa tggtcagacc
     2101 cgcacctacc gtttcgtgac cacgctccag gacaacaact ccgcgcaggg gctgagcgcc
     2161 aacacggtga gcttcacctg ggaagcccag aacacctaag cgaaaggcga ccgtggccct
     2221 tgacgaaggg gtcgcgccgc ccgcagcgta gagacgatgg acgcccgacc ggagacgaca
     2281 cgacgaggag cagccgactg gctggtcctc gtcggccgtt tcgcggcact ggtctacctc
     2341 aacgcgctgg tgtggctgtc cgtctgggtg ctgatcccca cgctcttcat gggctggcag
     2401 ccgatggcga tcgagaccgg gtcgatgggg ccgtcgatcc gcaccggcga cgtggtcctg
     2461 gtcgagtcct accgggggca gccgctggaa gccggccgcg tgatcacctt ccgggatccg
     2521 gccgacccgg accgcctggt gacgcaccgg atcaccgcgg tcaacgcgga cggcacgtac
     2581 ctgaccaagg gcgacgccaa cctcgacgcg gactccaccc cgctggccgg ggagcacatc
     2641 cgcggcgtcg gccgcctggt gatcccgcgc gtcggccttc cggtggtgtg gatggagcag
     2701 ggccactacg cggcgctggg cgcctgggcc gtcagcgtgg tgttcgcgct cgtcctgctc
     2761 gcgccctccc gtccggagga gcaaccgtcc cgggtgccgc tgtccgcacg cgtgccgagg
     2821 ttcccgcggc ggtcggcgcc cgcccccgtg gcgcccttgc ccgggcctgc ggtccccgtc
     2881 gcctccttgc cggtgccgcc tgggggcccg agcaggacgc tgcctgttgc tgccgggtgg
     2941 gcgcaggtgc cggcggtgcg ctaccgcctg cgcctgcccg tgccgcccat ggcgctggaa
     3001 ctgccggccc cgccgcgctg gacggtcgcg ctggtcccgt cgccgcccgg cacggcaccg
     3061 gtcgcgccca tcccggcgcc gccgcgcccg gccgccgtcc ggcgcggcac ggccctgtcg
     3121 agccgggacg tccccggatg ggcgggccga ctgcattcgt gatctcccgg tcgaacggga
     3181 cgcaacaccg ccgtatcgtg tggaaggggt gcccacgacg caggaggatc ggtcgtgcgg
     3241 cgccgggcgt ggatccgctt gctggtcgtg cttccgctgc tggcggcact ggccgtgctc
     3301 gacgtcagcg acgccgtgtt caccggcggc acgtccaacg gcggcagctc cttcgagggc
     3361 gacacgctgg ctcccccaag cggcgtgacc gtcacgcacc agtgcctgtt gagcatccag
     3421 tccgtgaagg tggactggac ggccagcccg tccacgtggg cgacgcacta ccgcgtgctg
     3481 cgcgcggtca acggcggcgc ctacgcctcc gtggcgacgg tgtcctacgg caccgacacg
     3541 tggacggaca gcggcgtgtc ggcgtccacg acgtacacgt accgggtgcg caccgagcgc
     3601 acgggatgga cgtggacgtc cgtggaggcc cagtccgccg acgtcaccac cccggcgctg
     3661 tgcctgtgac gccggcgctc cagccgcggc gcgaatccgc cgacatggag ggcacggccg
     3721 caagaccggc ttgactcgag cgtttccaga tgcggcaatg gtcagaccgc cgccgagggg
     3781 gatccaaccg gaggtgggtg tgcgggtgtc acgcccggtt gtcgcggcag gtctctccgg
     3841 cttgctgctc gtcaccatgc tcgtgctgcg cgtcagcggc gcggcgtttc aggccagcac
     3901 ggtcaacccc tccaacagtt tcggcgcggc caccgtggcc gtctcggaca acgacagcgg
     3961 caccctgctg ctggacacca cggcgatgct gcctggcgac accaagcaga cctgcgtcca
     4021 agtgagctac acgggcaccg tcgacgcgac ggtcaagctc cacggcaccg tctcgggctc
     4081 cctgccgcag tacctgaatc tgaccgtcga gcggggcagc ggcgccagcg ccttcggcaa
     4141 ctgcacgggc ttctcctcga ccgccaccgt cttcagcggc acggtctcag ggctcgcgtc
     4201 cagccacacg tcatgggccg acggcgccgg ctcctacacg gcggcaccct cctcgtcgca
     4261 gtggttccgc atcgtgtgga cgctccagga cgtcaacgcc gcacagggcc agaccgcgtc
     4321 ggcgacgtac acgtgggagg cgcgcagcgg cgcggtcacg accaacttcc gctccaccac
     4381 cgcgtcgtcg accgcgacgg tgccggccga cacgctccag ccgccgggca ctcccagcgc
     4441 cacctacaac tgcaccctga cggtccgcca gatcaccgtg agctggaccg cgacgccgag
     4501 tacctgggcg accacctacc gggtctaccg ctcggtggac ggcggtgcgt acagctcggt
     4561 ggccaccgtc gagtacggca cgacctcgtg ggtcgatacg gccgtcaacg gcggcacgac
     4621 gtacatctac aagctgcgca gcgagcgcac cggctggacc tgggtgtccg ccgacaccgc
     4681 ggtctccaac agcgtcacga ctccgacgct ttgcctgtga cgctcagcgc agccctcgcg
     4741 ccaccagcag ctcggcgatc tggacggtgt tgagcgcggc gcccttcagc aggttgtcgc
     4801 cgaccacgaa caggttcagc gcgtggtcgt cgcccaggtc ctcgcgcacg cggccgaccg
     4861 cgacctcgtc gcgccccgcc cactccagcg gcgtgggcac ctcgtccagc accaggcccg
     4921 ggaagccgtc catcgccgcc agcgccgcct cgcgcgtgac cggccgcgag aagcgggcgc
     4981 ggcactggac cgaatggccg accatgaccg gcacccgcac gcacgtggga ctgacccgca
     5041 ggtccgggat gcccaggatc ttgcggctct cgttctggag cttcagctcc tcgtcggtgt
     5101 agccgccctc gccgggcgtg cccagcagcg gcacgacgtt gtaggccagc gtcgcggcgt
     5161 gcaccttgtg ctcgaccagc ttcgcggccg cggcgccgtc cgcgcgcagc aggtccacgt
     5221 gctcgtgcag caccggcacc tgcgcggcca gctcgtcgat gcccttctgg ccggcgccgc
     5281 ccgccgcctg gtagctggtc gcgaccaagt ccaccaggcc gaaggcgtcg tgcagcgcct
     5341 tcagcggcag catgatcgcc atcgtggtgc agttggggtt ggcgacgatg cccttgccga
     5401 tgcgctccag cgcctcgggg ttcacctcgc tgaccaccag cggcacgtcg gggtccatgc
     5461 gccaggcgga gctgttgtcc accacgatcg cgccggcctc gaccgcgcgc ggcgcgtgct
     5521 ccctggaggt cccgccccct gcgctgaaca gggcgatgtc gacgccttcg aacaccgccg
     5581 ggtcctcgat ggagcgcacc gtgacgtcct ggccgcggaa ggtcagcacc ttgccctcgc
     5641 tgcgcgggct ggcgacgagg accacctcgt cgcaggggaa ggcgcgctgc tccagcaggc
     5701 ggcgcaggtc ctggccgacc gcgccggtgg cgccgacgac ggcgacccgc cgggacggac
     5761 tagccatggt cggcctccgc gaggttgaag cggtcgtgga cggcgcgcac tgctgcctcc
     5821 aggtcgctct cccggatcac cacgctgatg cggatggtgg aggtcgagat catctcgatg
     5881 ttgacaccaa cctcgctcag cgcctcgaac atggcggcgg ccacaccggg gtgggtcttc
     5941 atgccggcgc ccacgaggct caccttggcg atgccctcgt cgaagtcgac gcccgcggcg
     6001 ccgacctcgg cggccagctc gcgcaggatc tgcacggcct tggggccgtc ggccagcggc
     6061 agcgtgcagg agatgtcggt cgccccggac tcggacacgt tctgcacgat catgtcgacg
     6121 ttgatgtggt tggcggccag caccgtgaag atgcgcgcgg cgatgccggg cttgtccggc
     6181 acgccgcgga tcacgagctt gccctcgccg ctgtcgtggg cgacgcccgt gaccatcgcg
     6241 gcttccatgc cctcgtccat ctcgctgtca gacatgaccc aggttccctc gtcgtaggtg
     6301 aaagagctgc ggacgtgcac ggtgaccccg tggttgcgcg cgtactccac gctgcgcacc
     6361 tgcagcacct tggcgccgtg cgccgccagc tccagcatct cctcgtggct gacccgcgac
     6421 aggtggcggg cgtccggcac gatgcgcggg tcggcggtgt agacgccgtt gacgtccgtg
     6481 tagatctcgc acacgtcggc gcccagggcg ccggcgagcg cgaccgcggt ggtgtcgctg
     6541 ccgccccggc ccagcgtggt gacgtccttg gagtcgcggc tgacgccctg gaagccggcg
     6601 acgatcacca cgtgcccttc ctcaagagcc tcgtggatgc gcccgggcgt gatgtccatg
     6661 atccgcgcct tgccgtgcgc ggagtcggtg at
//
LOCUS       DATMKC010000315         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_217961, whole genome shotgun sequence.
ACCESSION   DATMKC010000315 DATMKC010000000
VERSION     DATMKC010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217961"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VNU01_09275"
     CDS             <1..248
                     /locus_tag="VNU01_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32847.1"
                     /translation="AGGLLFRGGETGWAVVAWALTFVAGAALRLASAGCRALAQLLER
                     SERIEEELRRLDREPPQAEPDSGRVPDPYRRWGGGWH"
     gene            362..1297
                     /locus_tag="VNU01_09280"
     CDS             362..1297
                     /locus_tag="VNU01_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32848.1"
                     /translation="MGTSFVEIQAGPLRAGDPAYLGSFQILGRIWHRASATAYVGVDP
                     LRRMAVVTLFRNHGRVETPREEFERSLLAAQRIQGRSVAPVLETGWEGALPWLAVRYV
                     PGRLAAELVAEPDLEPEELLAFAAATASGLHALHRAGVVHGDFGPHTVVLAERGPLVT
                     GFGGPAVHRGRRRGPWVGPEPANKVNAATDVYAWGWLVAAAALGADSVKFKELRRGSR
                     LLSTDLSSLPAPLPVIVEQALHPYPPSRPSAERIAQALPLRQWPPRRVPPVVLAPRSY
                     APPQPGAARRAAAVATILTVLALAVAAGALVAALR"
     gene            complement(1319..2737)
                     /locus_tag="VNU01_09285"
     CDS             complement(1319..2737)
                     /locus_tag="VNU01_09285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWH32849.1"
                     /translation="MSAEHDLPPDLDIAYPAVEPDADTTPGEHDTTMEAIVELCKRRG
                     LIFQSSEIYGGLRGAWDYGPLGAALKDNVKAAWKRAMIQLRDDVVQLDASILMHPKVW
                     EASGHVAGFTDPLVECRECNTRHRADHLEPNADGKMVCPNCGADALTEPKAFNLMFRT
                     FVGPTEDTSAQVWMRPETAQAMFVDFAHVQLTSRQKIPFGIAQVGKSFRNEITPRNFI
                     FRVREFEQMEMEYFVRPGTDEQWHEYWIQQRWHWYTRLGMRPENLRVREHGTDELSHY
                     AKRTVDVEYRFPFAWSELEGIANRADFDLTQHSKHSGKDLDYYDQERDERYIPFVIEP
                     AAGVDRTALAFLVDAYREEEAPTASGKVEKRTVLKLHHELAPLKAAVLPLSRNEKLTP
                     TARRVADLLRPHWLIQYDDAGSIGKRYRRQDEVGTPFCVTVDFETVEQDEAVTVRDRD
                     SMTQERVSIDRLVGYLQERLAL"
     gene            complement(2815..>3715)
                     /locus_tag="VNU01_09290"
     CDS             complement(2815..>3715)
                     /locus_tag="VNU01_09290"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWH32850.1"
                     /translation="ATCDLDQPMIFGRTPFAMPVRLGHECVAEVVDGPEGFSPGQRVV
                     VAFQISCGTCEPCRRGQTGNCASVPPLSMFGFGALGGDWGGMLADLALVPYAAAMLVP
                     LPDGVEPSAVASVADNVPDGWRTVAPGLAERPGCEVLVAGGGGSIPLYAVDAALALGA
                     ASVTYVDTDADRLAVAEALGARAVEGKPQRSLGVFPVTVDGTGTVDGLVATLRLTEWG
                     GRCTSIGQLAAEGGLPLFELYTRGVRLDLSRGMARPAMPALLDLVAAGRLRPQLVTTA
                     TVDWEDAPRALLEPATKLVFTRD"
BASE COUNT          453 a         1371 c         1372 g          519 t
ORIGIN      
        1 tcgccggcgg cctgctgttc cgcggcggcg agaccggctg ggccgtggtc gcgtgggccc
       61 tgacgttcgt cgccggcgcc gcgctccggc tggcctcggc cggatgccgc gcgctggcgc
      121 agctgctgga gcgcagcgag cgcatcgagg aggagctgcg ccgtctggac cgggagccgc
      181 cccaggccga gccggacagc ggccgcgtcc ccgacccgta ccgccgctgg ggcggaggct
      241 ggcactagcc agcgcgcccc cgccggccgg cggcgggcac ggtgctggcg ccccaccccg
      301 cgccctggca ccatgcgatt tgcgggtagg tcagcgacgg gcggccgggg cgagcatggc
      361 ggtgggcacg agtttcgtcg agatccaagc cgggccgctg cgcgccggtg atccggcgta
      421 cctggggtcc ttccagatcc tcggccggat ctggcatcgt gcgtccgcga ccgcctacgt
      481 gggcgtcgac ccgctgcggc gcatggcggt cgtcacgctg ttccgcaacc acggcagggt
      541 cgagacgccg cgcgaggagt tcgagcgcag cctgctcgcc gcccagcgca tccagggccg
      601 cagcgtcgcc cccgtgctgg agaccggctg ggaaggggcg ctgccgtggc tggccgtgcg
      661 ctacgtgccg gggcggctcg cggccgaact cgtggccgaa ccggacctgg agccggagga
      721 gctgctggcg ttcgcggccg cgaccgcgtc gggactgcac gccctgcacc gcgccggcgt
      781 ggtccacggc gacttcggtc cccacacggt cgtgctggcc gagcgcgggc cgctggtcac
      841 cggcttcggc ggacccgcgg tgcaccgcgg ccgccgtcgc ggcccctggg tcggcccgga
      901 gcccgccaac aaggtgaacg ccgccacgga cgtgtacgcg tggggctggc tggtcgccgc
      961 cgccgccctg ggcgccgaca gcgtgaagtt caaggagctg cggcgcggct cgcgcctgct
     1021 gtccacggac ctgtcgagcc tgcccgcccc cctgccggtc atcgtcgagc aggcgctgca
     1081 cccctacccg ccgtcgcgcc cctcggcgga gcgcatcgcg caagccctgc cgttgcggca
     1141 gtggccgccg cggcgggtgc cgccggtggt gctggcgccg cgcagctacg cgccgccgca
     1201 gccgggcgcg gcccgccggg cggctgccgt ggcgaccatc ctgacggtgc tggcactggc
     1261 cgtcgccgcc ggggcactcg tcgcggcact gcgctagccc cccacgctca ggcggcggtc
     1321 agagggccag gcgctcctgg aggtagccca ccaggcggtc gatggacacg cgctcctgcg
     1381 tcatcgagtc gcggtcgcgc accgtcacgg cctcgtcctg ctcgaccgtc tcgaagtcca
     1441 cggtcacgca gaagggcgtg ccgacctcgt cctggcggcg gtagcgcttg ccgatggagc
     1501 cggcgtcgtc gtactggatc agccagtgcg ggcgcagcag gtccgcgacg cgccgcgcgg
     1561 tcggggtcag cttctcgttg cgcgacagcg gcagcaccgc cgccttcagc ggcgcgagct
     1621 cgtggtgcag cttcagcacg gtgcgcttct cgaccttgcc ggaggcggtc ggcgcctcct
     1681 cctcgcggta ggcgtccacc aggaacgcca gggccgtgcg gtccacgccg gccgccggct
     1741 cgatcacgaa cgggatgtag cgctcgtcgc gctcctggtc gtagtagtcc aggtccttgc
     1801 cgctgtgctt ggagtgctgg gtcaggtcga agtcggcgcg gttggcgatg ccctccagct
     1861 cgctccacgc gaacgggaag cggtactcca cgtccaccgt gcgcttggcg tagtggctga
     1921 gctcgtcggt gccgtgctcg cgcacgcgca ggttctccgg gcgcatgccc aggcgcgtgt
     1981 accagtgcca gcgctgctgg atccagtact cgtgccactg ctcgtcggtg ccggggcgga
     2041 cgaagtactc catctccatc tgctcgaact cgcgcacgcg gaagatgaag ttgcgcggcg
     2101 tgatctcgtt gcggaaggac ttgccgacct gcgcgatgcc gaaggggatc ttctggcggc
     2161 tggtgagctg cacgtgggcg aagtccacga acatcgcctg agccgtctcc gggcgcatcc
     2221 acacctgggc gctggtgtcc tcggtcgggc ccacgaacgt gcggaacatg aggttgaacg
     2281 ccttgggctc cgtcagggcg tcggcgccgc agttggggca caccatcttg ccgtcggcgt
     2341 tcggctccag gtggtcggcg cggtggcgcg tgttgcactc gcggcactcc accagcgggt
     2401 cggtgaagcc ggcgacgtgg ccggaggcct cccacacctt ggggtgcatg aggatcgacg
     2461 cgtcgagttg cacgacgtcg tcgcggagct ggatcatcgc ccgcttccac gcggccttga
     2521 cgttgtcctt gagggcggcg ccgagcgggc cgtagtccca cgcgccgcgc aggccgccgt
     2581 agatctccga ggactggaag atcagcccgc ggcgcttgca cagctcgacg atggcctcca
     2641 tcgtcgtgtc gtgctcgccg ggtgtcgtgt cggcgtccgg ctccaccgcc gggtaggcga
     2701 tgtccaggtc cggtggcagg tcgtgttcgg cgctcatcgt cggatgtccc ctcatgcaag
     2761 accccccaag tggctctcgg ggggtccgac gaggctaaca cgccgcgagc cgggtcagtc
     2821 gcgcgtgaag accagtttcg tggccggctc cagcagcgcc cggggcgcgt cctcccagtc
     2881 cacggtcgcc gtggtgacga gctgggggcg cagccggccg gcggccacga ggtccaggag
     2941 ggccggcatc gccggacggg ccatgccgcg gctcaggtcc aggcgcaccc cacgcgtgta
     3001 cagctcgaac agcggcaggc cgccctcggc ggcgagctgg ccgatcgagg tgcagcgccc
     3061 gccccactcg gtcagccgca gcgtggcgac caggccgtcg acggtcccgg tgccgtcgac
     3121 ggtgacgggg aacacgccca gggagcgctg aggcttgccc tcgaccgcgc gcgcgccgag
     3181 cgcctcggcc accgccagcc gatccgcgtc cgtgtccacg taggtcaccg aggcggcgcc
     3241 gagcgccagc gcggcgtcca ccgcgtagag cgggatggag cctccgccgc cggccacgag
     3301 cacctcgcac cccggccgct ccgccagtcc gggcgcgacc gtgcgccagc cgtcgggcac
     3361 gttgtcggcg acgctcgcca ccgcgctcgg ctcgacgccg tccggcagcg gcacgagcat
     3421 ggccgcggcg tagggcacca gcgccaggtc ggccagcatg ccgccccagt ccccgcccag
     3481 ggcgccgaag ccgaacatcg acagcggcgg cacgctggcg cagtttccgg tctggcccct
     3541 ccggcacggc tcgcacgtcc cgcaggagat ctggaacgcc accacgacgc gctgccccgg
     3601 tgagaacccc tccgggccgt cgacgacctc cgccacgcac tcgtgcccca ggcgcaccgg
     3661 catggcgaac ggcgtgcggc cgaagatcat cggctggtcg aggtcacagg tcgcc
//
LOCUS       DATMKC010000316         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_318049, whole genome shotgun sequence.
ACCESSION   DATMKC010000316 DATMKC010000000
VERSION     DATMKC010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318049"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VNU01_09295"
     CDS             complement(<1..1375)
                     /locus_tag="VNU01_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32851.1"
                     /translation="MSWDRMPFGERDAERDRLLAQWLATEVVPFNGYWSKRLGGKAPR
                     RLAELADLAPLEEAEAAGLGGPGNPALLLLPTEDGFKRHATRGEIFRAALESRQGGAR
                     ARRSILFRRYKPVHVHDAGVAALIAVAYTRTDLDRLHLAGARLMEVLGLGADDRLVNA
                     VPAGPSIRFWGLYHAALAARMTALHPRAAGGDPVAAVARALALLPPTVLAAPVDEALG
                     ILQGLGERGIRCLSLRTVLTVGPPPTPQRREEIGEAAAALGASGVRVQAVWAPETSRV
                     LWGECRPGTADPWEATYGLHTYPDLEIVEVRDTERGRPANPGDPGEILLTSLGWRGTA
                     LVRMATGSWTAGLAGSAVCPNCKRTVPRLAPDAVEAAWQPRVRVGDRLTRVDVRHASR
                     VLTPSVRERLGIASWSLRPVDGELTLVVETRDGGTAGLADLARDIGEAVGVVPVVRLT
                     RPAGGVQR"
     gene            complement(1670..>2163)
                     /locus_tag="VNU01_09300"
     CDS             complement(1670..>2163)
                     /locus_tag="VNU01_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWH32852.1"
                     /translation="AEESGGSAETVQLQVEVTEEAKRAKVVDTVEQSAGGRPDVTEAA
                     IIVAGGRGLGNAEGFGLIEQLADALKAGVGASRAATDAGWYPHSHQIGQTGKTVSPQL
                     YIGAGISGAIQHRAGMQTSQTIIAINKDPEAPIFSIADFGIVGDLYKVIPPLIDEINA
                     RKG"
BASE COUNT          278 a          806 c          818 g          261 t
ORIGIN      
        1 cccgctgcac gccgcccgcc gggcgcgtca ggcgcaccac cggcaccacg ccgacggcct
       61 cgccgatgtc gcgcgcgagg tcggccaggc cggccgtgcc gccgtcgcgg gtctccacca
      121 ccaaggtgag ctcgccgtcg accggccgca gcgaccagct ggcgatgccg aggcgctccc
      181 gcaccgacgg ggtgagcacg cgagaggcgt ggcgcacgtc gacgcgggtg agccggtcgc
      241 cgacgcgcac gcgcggctgc caggccgctt ccacggcgtc cggcgccagc ctcggcacgg
      301 tgcgcttgca gttggggcac acggcgctgc ccgcgagccc ggccgtccac gaccccgtgg
      361 ccatgcggac gagcgcggtg ccgcgccagc cgaggctggt gagcaggatc tcgccggggt
      421 cgcccgggtt ggcgggacgg ccgcgctcgg tgtcccggac ctcgacgatc tccaggtcgg
      481 gataggtgtg caggccgtag gtcgcctccc aggggtcggc cgtccccggg cggcactccc
      541 cccacagcac ccgggaggtc tccggcgccc acaccgcctg cacgcgcacg ccgctggccc
      601 cgagggccgc ggcggcctcg ccgatctcct cgcggcgctg cggcgtgggc ggggggccca
      661 cggtgagcac cgtgcgcagg ctgaggcagc ggatgccgcg ctcgccgagc ccttgcagga
      721 tgcccagggc ctcgtcgacc ggggcggcca gcaccgtcgg gggcagcagc gcgagggcgc
      781 gggccacggc cgcgaccggg tcgccgcccg cggcgcgcgg atgcagcgcc gtcatgcggg
      841 ccgccagcgc cgcgtggtac aggccccaga accggatcga gggaccggcg ggcaccgcgt
      901 tgaccaggcg gtcgtcggcg ccgaggccga gcacctccat gagccgcgcg ccggccaggt
      961 gcaggcgatc gaggtcggtg cgcgtgtagg cgaccgcgat cagcgcggcg acccccgcgt
     1021 cgtggacgtg gacgggcttg tagcgccgga acaggatcga ccggcgcgcc cgcgccccgc
     1081 cctggcggga ctccagcgcc gcgcggaaga tctcgccgcg cgtggcgtgg cgcttgaagc
     1141 cgtcctcggt gggcagcagc agcagcgccg ggttgccggg gccgcccagc ccggcggcct
     1201 cggcttcctc cagcggcgcg aggtcggcga gctcggccag gcggcggggc gctttgcccc
     1261 cgagccgctt cgaccagtac ccgttgaacg ggacaacctc cgtggcgagc cactgcgcga
     1321 gcaggcggtc gcgttcggcg tcccgctcgc cgaacggcat gcgatcccaa gacatgccga
     1381 cgagcatagg gcccatccac agattccccg cctatgcggg tgcactccgg gcagcatgac
     1441 gcccaatctg cacccgaccc ccagcgtcac gaagccacgg atcgaagaac agcaaaggcg
     1501 ccggaaaggg ctgggcaggc gcggctggaa cgaacacggc ccggtgggcg ctcaccgcgc
     1561 tggccgccgc aggcggctgg ccgtacaacg gggaagggcc gccgcccggc ggaggagcgt
     1621 aaaaggctct tgctccgacg ccgggatggc ggcccttccg ttgggacgat cagcccttgc
     1681 gggcgttgat ctcgtcgatc agcggcggga tcaccttgta gaggtcgccc acgatgccga
     1741 agtcggcgat ggagaagatc ggcgcctccg ggtccttgtt gatcgcgatg atcgtctgcg
     1801 acgtctgcat gccggcgcgg tgctggatcg ccccggagat cccggcgccg atgtacagct
     1861 gcggcgacac cgtcttgccg gtctggccga tctgatggct gtgcgggtac cagcccgcgt
     1921 ccgtggcggc gcgggacgcg ccgacgccgg ccttgagcgc gtcggcgagc tgctcgatga
     1981 gtccgaagcc ctccgcgttg cccaggccgc ggccgccggc gacgatgatc gccgcctccg
     2041 tgacgtcggg gcggccgccg gcggactgct ccacggtgtc gacgaccttg gcgcgcttgg
     2101 cctcctcggt cacctccacc tgcagctgca cggtctcggc ggagccgccg gactcctctg
     2161 cgg
//
LOCUS       DATMKC010000317        12370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_212219, whole genome shotgun sequence.
ACCESSION   DATMKC010000317 DATMKC010000000
VERSION     DATMKC010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212219"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VNU01_09305"
     CDS             <1..409
                     /locus_tag="VNU01_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWH32853.1"
                     /translation="EDPAASRVEVDIDVASVRTDSDQRDGHLLSPDFFDADNYAQIRF
                     ASTKVEPVDGDTWNLHGDLTIKDVTRPVTLTFEYLGVLKDPWGNDKAAFSAFTEVDRE
                     QWGLTWNAALETGGVLVGKQVRLEFEIQAQLAA"
     gene            561..1049
                     /locus_tag="VNU01_09310"
     CDS             561..1049
                     /locus_tag="VNU01_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32854.1"
                     /translation="MRGNKTKLLVACALAGALAAPVGAAAQTTTDSASHPVKEHPGQD
                     TREHRGEALSLDCKWFRHDGREGVGCRWTPSKARDFAAYRLVRGERGQEPVVVFETDD
                     RHQTHALDATAARDRDYSYMVLVFDAAGEVIGHTNPVRVSTRGRPDRPRPPAALPAVP
                     AL"
     gene            1103..1702
                     /locus_tag="VNU01_09315"
     CDS             1103..1702
                     /locus_tag="VNU01_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWH32855.1"
                     /translation="MEAGAGMRDLDERELVERARHDADAFAALYRRYVERIHAFAYRR
                     TGSREIAEDVTAATFEKALRYLHTFDWRTGGFAPWVFRIAANELATHYRKEYRTKTAK
                     AQATFRTLQPSVDDGGYDEIERRLDEPVGQVRLAMDRLSPRYQEALTLRFLSGLSLDQ
                     AAAAMRCSKPAMSVTVHRALKALREAMVAPGQTAGKVER"
     gene            1726..2763
                     /locus_tag="VNU01_09320"
     CDS             1726..2763
                     /locus_tag="VNU01_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32856.1"
                     /translation="MQELGRGRAPRPDDAFTDALEQRLRAVHATLPAAGHEAGATTHP
                     PAAAGRAWWRSPVPVAAGLVLLLTISFGAVLNRPAGVELAAAYDATVTLPDGRLVQAR
                     PGQRLAEGAVVLTGPNGHLLAGGVRLGPGEMAVVRDHRLQPVHEAQPERRHPAAPAEP
                     PRRRPIAVVPSLPAMPRPGSPEPRPTRPQQPPSAQPEPGPTADPRRPPGDEPAPGEPG
                     VHAGRQEPDAQHAMGLAATADEGRVFLSWTEFRDPRFAFFVILRASETAPTWPPSEGT
                     ELVGRSADPERRGFADGAPPRGAVYRVVAVDQDGRELARSGAVAPAPMEASGGRHASD
                     GTEQTVAPTRG"
     gene            2833..3234
                     /locus_tag="VNU01_09325"
     CDS             2833..3234
                     /locus_tag="VNU01_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWH32857.1"
                     /translation="MSAQDFRAGVESLDLDAMTAALADDVVLHSPVTFKPFQGKAAVR
                     RLLGILLETFSDFRYTDELRGDGSHILVFRARVGDRDVEGIDLLRAGPDGLVQDFTVM
                     VRPLSAAIALAEAVGPKLAAAGPAGAAGTSA"
     gene            3253..5844
                     /gene="pepN"
                     /locus_tag="VNU01_09330"
     CDS             3253..5844
                     /gene="pepN"
                     /locus_tag="VNU01_09330"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWH32858.1"
                     /translation="MPETHPNLTRDEARQRARLLSDVVYTVDLDVTGDGDTFGSRTVV
                     RFDCTEPGASTFVDLVAPAVRRITLNGRDVGPAAHRDHRIALEGLAASNELIVEADCA
                     YMRTGVGLHRFADPVDGKVYLHTQFEPFDAHRVFACFDQPDLKAPLELSVRAPAEWLC
                     VSNTAAVEQPPRGQAGVWRFAPTLPISPYIYALVAGPYHQVHDRWDGGGGEPVDLGIY
                     CRASLAPHLDAEEIFTITKQSFAFFTETFGYPYPFGQGGGHPGPSKKYDQLFVPEFNF
                     GAMENPGCVTFSESYLFRSKVTDAARESRAGTITHEMAHMWFGDLVTMRWWDDLWLNE
                     SFATYIGTLAVAESTRFTEAWSEFCNQLKAWAYREDQLPSTHPIVADITDTEAVRTHF
                     DGITYAKGASVLKQLVAWVGREGFVEGLRDYFRRHAFGNTTLEDFLGALDRASGRELS
                     DWSKLWLETAGVNTIRPEIRADDGVYGEVALLQEAPERWPTLRPHRIALGLYDRQGGK
                     LVRRERVELDVEGSRTVVPELAGRRVPDLLLINDDDLAYTKVRLDERSLRTVEEHLDE
                     LPGSLPRALVWGAAWDMTRDGELATRRFVRLVARHVGAESNVGVLQVLLRHASTATDA
                     YGRPDNRHAMHALLADVARRELERAEPGSDFQLQWARTLADNCVRPEDQAFLLALLSG
                     AESIRGLDIDTELRWHFLVDLASEGAADEGAIAAELERDPTDIGQRRAATARAARPDA
                     DAKKEAWAALVERRDVSLARMRALVGGFHQVGQDALLRPYVQRYVDALPGIWSTRGPE
                     EALLLTEGLFPGALVEPATVEAAERALALGLPAPARRILEESQDGVRRALRAREADTE
                     TGARARA"
     gene            5874..6686
                     /locus_tag="VNU01_09335"
     CDS             5874..6686
                     /locus_tag="VNU01_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWH32859.1"
                     /translation="MDEVIARGTAVPALGYGTWELEGDDCLRGVAAALELGYRHLDTA
                     QVYGNEDLVGQALADSGVAREEVFLTTKVWNSRLEPERVRSTTEDSLRRLRTDYVDLL
                     LIHWPVRMDILPQTLEAMRGLQERGLVRHVGVSNFTPAQLREALAHAEVFCNQVEYHP
                     YLGQAALLEVCREQDVLLTAYSPLARGRVLEDPVLREIGEAHGKSAAQVTLRWLVQQP
                     GVATIPKAASRRHIESNLAIFDFALSDAECARIAALERGERIVDPDWADWAA"
     gene            6703..7059
                     /locus_tag="VNU01_09340"
     CDS             6703..7059
                     /locus_tag="VNU01_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32860.1"
                     /translation="MPIATQLAGFVLTMIGVGTFLATGAEEPTALIPALFGVALWGAG
                     FVAERDEAKRMHAMHAAMLVALLGALATAPTALGVGAMGDASESARIESWLTVIVCLV
                     YLAGGVRSFVAARRSR"
     gene            7115..8188
                     /locus_tag="VNU01_09345"
     CDS             7115..8188
                     /locus_tag="VNU01_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32861.1"
                     /translation="MPVAYAASVWARALSFLLLTGLAAACTAEEAMDSAFQSLSRNPP
                     PGAATPETGDEGDAGTEQPSACPDGERCFGRPARTGAFDVAEVPEASGAARSLRDPGV
                     LWLVDDRPGQAELLAVRPDGSAAGRLRLAGAETVNVEALAAGPCEAGGDAPCLYIADI
                     GDNTRSRADVRVLRVPEPDLAGGPAPAATPDVIALRYPDQPHDAEALLVDAGGVPYVI
                     TKAPFDAASGVTGATRLFAAPGFRDGLLLDLGAVPLPEPEVAVATLFYGNTVTGADSA
                     PGRVLLRTYDQAVEYVAPSPDADLAGFAAWPHQRVPAPWQPQAEAVAYAGDHCGYYTL
                     SEGSGDIWLVPCEGTDAGGRDAP"
     gene            8211..9005
                     /locus_tag="VNU01_09350"
     CDS             8211..9005
                     /locus_tag="VNU01_09350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH32862.1"
                     /translation="MSTTWRRALVTGASSGIGRAIALEAAARGADVVLVARRASMLQD
                     VADTVRQAHGRAAEILVADLTAQDGLDAVEARLRDESSPIDLLVNNAGFGTFGTFHRL
                     PVAREDEEIRLNVLALVRLSHAALSAMVPRGHGGVLNVSSSAGFQAMPYSATYCSTKA
                     FVNTFTECLHEEAKGAGVHVSALCPGYVRTEFQDVANVSTSVIPKFAWLDVHAVARQG
                     LDAVSGNDALSVTGGLYRAGIALNRLLPRAAVRRIAGAVASRRMKL"
     gene            complement(9002..9718)
                     /locus_tag="VNU01_09355"
     CDS             complement(9002..9718)
                     /locus_tag="VNU01_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWH32863.1"
                     /translation="MRTLLKPKWIAGHLLAVVVVVTFANLGLWQLRRHDWRAERNDRI
                     AQGLAREPAPFASLPEGERAYRPVTVSGRYRPDADVLLTPRNELGPGHHVLTPLLLDD
                     GTALLVDRGWVPFRHATPPVPGAAAAAGPVRLEGVLLPAAEALPQGVREGGRLVRVRA
                     VDPDAVLDAVLGGQARAVEDVFLLLREQQPPPGPLPVPGPVPEQDAGPHLSYAIQWFL
                     FAAVVVAGYPLLLRRAAEGV"
     gene            9808..10659
                     /locus_tag="VNU01_09360"
     CDS             9808..10659
                     /locus_tag="VNU01_09360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWH32864.1"
                     /translation="MKFGITMFPTDEAMRPDELAIAVEDRGFDSLWFPEHTHIPASRD
                     TPYPGGGDLPREYARTHDPLVALAFAAGVTERLLLGTGVCLVVERDPIVLAKEVASLD
                     ALSGGRVLFGVGAGWNREEMRNHGTDPATRFELLRERVEAMKQIWTQDEATYHGDFVN
                     FDRVWSWPKPVRRPHPPIVIGGAGASALQRVVDYGDEWMPIARGWGAGDLARRVAELR
                     RLAQEAGRGHIPVTAYSASSKPESVEQFAEAGVERLVFWAPPAPADQVLPLLDRYAPL
                     VERYAGA"
     gene            10708..11337
                     /locus_tag="VNU01_09365"
     CDS             10708..11337
                     /locus_tag="VNU01_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWH32865.1"
                     /translation="MDLHLSHPPRSLDGFPERRLEAGARLWTVHPDDRWPWSCAADGD
                     GRFDLEEPWGTCYLSMDPLGALIECCCRRSPEVDEDALLQARLSVMRLDRDLRLADVA
                     HPRSFGYGVTAEVHSIGDYAATQAWARAFREAGFDGVHYLLRHDPSRSLRGVARFGPA
                     GERREWPRPDTGRFGEGLLREAAERFGIRYSAARTSAAARSPDSTAPSM"
     gene            complement(11274..12248)
                     /locus_tag="VNU01_09370"
     CDS             complement(11274..12248)
                     /locus_tag="VNU01_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH32866.1"
                     /translation="MPLDRPWDAPDAEELDGLTFETWIRRTCKTDGGRRFFRVVSAAV
                     WAADPASLSMLHVLFYVHSAGGLDALLDTGGGAQQDRVVGGTQLLAVRLAERLGPGRV
                     RLATPVRRVEWGPGGVSLHADGGVEVHARRAILTGPPTLLGRLSYDPPLPAERDLLTQ
                     RVPNGAVVKCMAVYERPFWRDAGLSGQAASDEGPVQVVFDNSPPSGTPGVLLGFLEGT
                     HAIRLSGVPADERRAAVVDAFTGLFGPQAAQPLRYIERDWSAEEWTRGCYGGHMPPGA
                     WTQLGPALARPVGPLHWAGSETGRRWSGYMDGAVESGERAAAEVLAAL"
BASE COUNT         1624 a         4588 c         4644 g         1514 t
ORIGIN      
        1 cgaggacccg gcggcctcgc gcgtcgaggt cgacatcgac gtggccagcg tgcgcaccga
       61 cagcgaccag cgcgacggcc acctgctgtc gcctgacttc ttcgacgccg acaactacgc
      121 gcagatccgc ttcgcgagca ccaaggtgga gcccgtggac ggcgacacct ggaacctgca
      181 cggcgacctc accatcaagg acgtcacccg cccggtgacg ctcaccttcg agtacctggg
      241 cgtgctgaag gacccgtggg gcaacgacaa ggccgcgttc tccgcgttca ccgaggtgga
      301 ccgcgagcag tggggcctga cctggaacgc cgcgctggag accggcggcg tcctggtggg
      361 caagcaggtc cgcctggagt tcgagatcca ggcgcagctc gccgcctgac acgccccctc
      421 ccgccctctc ccaacctgga acgttcgtcc gatatctcgg acgaacgttc cacattggga
      481 aggtgtaagc accgcacccg ccacgcgttg tcctgctcgg ggagtgcccg tcggcacgac
      541 gacgagagcg ggaggacgac atgcgaggca acaagacgaa gctcctggtc gcgtgtgcgc
      601 tggccggcgc actggccgcg cccgtgggcg ccgcggcgca gaccaccacg gacagcgcga
      661 gccaccccgt gaaggagcat cccggccagg acacccggga gcaccgcggt gaggcgctgt
      721 ccctggactg caagtggttc cgccacgacg gccgcgaggg cgtcggctgc cgctggacgc
      781 ccagcaaggc ccgcgacttc gccgcctacc ggctggtgcg tggcgagcgc ggccaagagc
      841 ccgtcgtggt gttcgagacg gacgaccgcc accagacgca cgcgctggac gccacggccg
      901 cgcgcgaccg tgactactcc tacatggtcc tggtcttcga cgccgccggc gaggtgatcg
      961 ggcacaccaa ccctgtgcgc gtgtccacgc gcggccgccc ggaccggccg cgcccgccgg
     1021 ccgcgctgcc cgccgtcccg gcgctgtagc gcccgtggcc ggtgcgctgg gggtggcgag
     1081 gcgtggggca gactgtgccc gcatggaggc gggcgcgggt atgcgggacc tcgacgagcg
     1141 cgagctcgtc gagcgcgccc gccacgacgc cgacgccttc gccgccctct accgccggta
     1201 cgtcgagcgc atccacgcgt tcgcgtaccg gcgcaccggc tcgcgggaga tcgccgagga
     1261 cgtcaccgcg gcgacgttcg agaaggccct gcggtacctg cacacgttcg actggcgcac
     1321 cgggggcttc gcgccgtggg tcttccgcat cgcggcgaac gagctggcca cccactaccg
     1381 caaggagtac cggaccaaga cggccaaggc gcaggcgacg ttccgcacgc tgcagccgtc
     1441 cgtggacgac ggcggctacg acgagatcga gcggcgcctg gacgagccgg tcggacaggt
     1501 gcgcctggcg atggaccggc tgagcccgcg ctaccaggag gcgctcacgc tgcgcttcct
     1561 gtccggactg tcgctggacc aggccgccgc ggcgatgcgc tgctccaaac ccgcgatgtc
     1621 ggtgacggtg caccgggcgc tcaaggcgct gcgcgaggcg atggtcgcgc ctggccagac
     1681 cgccgggaag gtcgagcgat gacacgcgac gagacgagac gccgcatgca ggagctggga
     1741 cgcggtcggg cgccgcgccc cgacgacgcg ttcaccgacg ccctggagca gcggctgcgg
     1801 gccgtgcacg cgacccttcc ggccgctgga cacgaggccg gcgccaccac gcatcccccc
     1861 gcggcggcag gccgcgcgtg gtggcgctcc cccgtgcccg tcgcagccgg gctggtgctg
     1921 ctgctgacga tctcgttcgg cgccgtgctg aaccggccgg cgggcgtgga gctggcggcc
     1981 gcctacgacg cgaccgtgac gctgcccgac gggcgcctcg tgcaggcgcg tcccggccag
     2041 cgcctggccg agggcgccgt ggtgctcacc ggccccaacg gccaccttct cgcgggcggc
     2101 gtgcggctcg gaccggggga aatggccgtc gtgcgcgacc accggctcca gccggtgcac
     2161 gaggcgcagc cggagcggcg gcaccctgcc gccccggccg agcccccgcg gcggcggccg
     2221 atcgccgtgg tgccctcgct gcccgccatg ccgcgacccg gctcgccgga gccccggccc
     2281 acccggccgc agcagccgcc gtccgcccag ccggagcccg gcccgaccgc cgatcctcgc
     2341 cgcccgccag gcgacgagcc ggccccgggt gagccgggag tgcacgccgg ccgccaggaa
     2401 cccgatgcgc agcacgccat ggggctggcc gcgacggcag acgagggacg cgtcttcctg
     2461 agctggacgg agttccgtga cccccgtttc gccttcttcg tgatcctccg cgcgagcgag
     2521 acggcgccga cgtggcctcc gtccgagggc accgagctgg tgggccgctc ggccgacccg
     2581 gagcgacgcg gcttcgccga cggcgcgcca ccgcgcggcg cggtgtaccg cgtggtggcc
     2641 gtggatcagg acggccgcga gctcgcccgc agcggcgcgg tggcgcccgc acccatggag
     2701 gcctccgggg gccggcacgc ctcggacggc accgagcaga cggtggcgcc caccaggggc
     2761 taggctcggc gctcgcgcgg ccacggacgg ccgccgggca ggccgcccct acggccgcgg
     2821 gaaggagccg ggatgagcgc gcaggacttt cgcgcgggtg tggagtcgct ggacctcgac
     2881 gcgatgacgg cggcactggc cgacgacgtg gtgctgcact cgccggtgac cttcaagccg
     2941 ttccagggca aggccgccgt gcgccggctg ctgggcatcc tgctggagac cttctccgac
     3001 ttccgctaca ccgacgagct ccgcggcgac ggcagccaca tcctggtgtt ccgggcccgc
     3061 gtgggcgacc gcgacgtcga gggcatcgac ctgctgcgcg ccgggccgga tgggctggtc
     3121 caggacttca ccgtcatggt gcggccgctg tcggcggcga tcgccctggc cgaggcggtg
     3181 ggccccaagc tggccgccgc ggggccggcc ggcgccgcgg gcacatccgc ctgacagggt
     3241 aggctcgccg ccatgcccga gacccatccc aacctcaccc gcgacgaggc ccggcagcgg
     3301 gcgcggctgc tctccgacgt cgtctacacg gtcgacctgg acgtcaccgg tgacggcgac
     3361 accttcggca gccggacggt cgtgcgcttc gactgcaccg agccgggcgc gtccacgttc
     3421 gtcgacctgg tcgcccccgc cgtgcgccgc atcacgctga acgggcgcga cgtgggaccc
     3481 gccgcccacc gcgaccaccg catcgcgctg gagggcctgg ccgcgtccaa cgagctgatc
     3541 gtggaggccg actgcgcgta catgcgcacg ggggtgggcc tgcaccgctt cgccgacccg
     3601 gtcgacggca aggtgtacct gcacacgcag ttcgagccgt tcgacgccca ccgcgtgttc
     3661 gcgtgcttcg accagccgga cctgaaggcg ccgctggagc tgtccgtgcg cgcgccggcc
     3721 gagtggctct gcgtcagcaa taccgccgcg gtggagcagc cgccgcgcgg ccaggccggc
     3781 gtgtggcgct tcgccccgac gctgccgatc tcgccctaca tctacgccct ggtcgccggc
     3841 ccgtaccacc aggtgcacga ccgctgggac ggcgggggcg gcgagccggt ggacctcggc
     3901 atctactgcc gcgcctcgct ggcgccgcac ctggacgccg aggagatctt cacgatcacc
     3961 aagcagagct tcgcgttctt caccgagacc ttcggctatc cctacccgtt cggccagggg
     4021 ggcgggcacc cgggcccttc aaagaagtac gaccagttgt tcgtgccgga gttcaacttc
     4081 ggcgccatgg agaaccccgg ctgcgtcacg ttctccgaga gctacctgtt ccgctccaag
     4141 gtgaccgacg ccgcgcggga gtcccgcgcg ggcacgatca cccacgagat ggcgcacatg
     4201 tggttcggcg acctggtcac catgcgctgg tgggacgacc tgtggctgaa cgagagcttc
     4261 gccacctaca tcggcacgct ggccgtggcg gagtcgaccc gcttcaccga ggcgtggtcg
     4321 gagttctgca accagctcaa ggcgtgggcc taccgcgagg accagctccc gtccacgcac
     4381 ccgatcgtgg cggacatcac cgacaccgag gcggtgcgca cgcatttcga cggcatcacc
     4441 tacgccaagg gcgccagcgt gctgaagcag ctggtggcct gggtgggccg cgagggcttc
     4501 gtcgaggggc tgcgcgacta cttccgccgc cacgccttcg gcaacaccac gctggaggac
     4561 ttcctgggcg cgctggaccg cgccagcggc cgcgagctgt cggactggtc gaagctctgg
     4621 ctggagaccg cgggggtcaa cacgatccgt cccgagatcc gtgcggacga cggcgtctac
     4681 ggggaggtgg cgctgctcca ggaggcgccc gagcgctggc cgacgctgcg cccgcaccgc
     4741 atcgcgctgg gcctgtacga ccggcagggc ggcaagctgg tgcgccgcga gcgcgtcgag
     4801 cttgacgtgg agggctcccg gaccgtggtg cccgagctgg ccggcaggcg cgtgccggat
     4861 ctgctgctga tcaacgacga cgacctcgcc tacaccaagg tccgcctcga cgagcgctcg
     4921 ctgcggaccg tcgaggagca cctggacgag ctgccgggat ccctgccgcg cgcgctggtg
     4981 tggggcgcgg cctgggacat gacgcgcgac ggcgagctgg ccacgcggcg cttcgtgcgg
     5041 ctggtggcgc ggcacgtcgg cgcggagtcc aacgtcggcg tcctgcaggt cctgctgcgc
     5101 cacgcctcca ccgccacgga cgcctacggc aggccggaca accgccacgc gatgcacgcg
     5161 ctgctcgccg acgtggcgcg ccgcgagctg gagcgcgccg agccgggcag cgacttccag
     5221 cttcagtggg cgcggacgct ggccgacaac tgcgtgcgcc ccgaggacca ggcgttcctg
     5281 ctggcgctgc tgtccggtgc cgaatcgatc cgggggctgg acatcgacac cgagctgcgc
     5341 tggcacttcc tggtggacct ggcctccgag ggcgcggcgg acgagggcgc gatcgccgcc
     5401 gagctggagc gcgaccccac cgacatcggc cagcggcgcg cggcgacggc ccgggccgcc
     5461 cgccccgacg ccgacgccaa gaaggaggcc tgggccgcgc tggtcgagcg ccgcgacgtc
     5521 tccctggcgc ggatgcgcgc actggtcggg ggcttccacc aggtgggtca ggacgcgctg
     5581 ctgcggccgt acgtgcagcg ctacgtggac gcgctgccgg ggatctggtc cacgcgcggc
     5641 cccgaggagg cgctgctgct caccgagggg ctgttcccgg gggcgctggt cgagcccgcc
     5701 accgtggagg ccgcggagcg cgcgctcgcg ctcggcctgc cggccccggc caggcgcatc
     5761 ctggaggagt cccaggacgg cgtgcgccgt gccctgcgcg cgcgcgaggc cgacacggag
     5821 accggcgcgc gggcgcgggc atgaacggcg acggcagcgg tgaggaatcg cgcatggacg
     5881 aggtgatcgc acgaggaacg gccgtgccgg cgctgggcta cggcacctgg gagctggagg
     5941 gcgacgactg cctgcgcggc gtcgccgccg cgctggagct gggctaccgc cacttggaca
     6001 ccgcccaggt ctacggcaac gaggacctgg tcggccaggc cctggccgac agcggcgtgg
     6061 cgcgcgagga ggtgttcctg accaccaagg tctggaacag ccgcctggag ccggagcgcg
     6121 tgcgcagcac caccgaggac agcctgcgca ggctgcgcac ggactacgtg gacctgctgc
     6181 tgatccactg gccggtgcgc atggacatcc tgccgcagac cctggaggcc atgcgcgggc
     6241 tgcaggagcg cgggctggtg cgccacgtcg gcgtgagcaa cttcacgccc gcgcagcttc
     6301 gcgaggcgct ggcgcacgcc gaggtgttct gcaaccaggt ggagtaccac ccctacctcg
     6361 gccaggcggc gctgctggag gtctgccgcg agcaggacgt gctcctgacc gcctactcgc
     6421 cgctggcgcg cggccgggtg ctggaggacc cggtgctgcg ggagatcggc gaggcgcacg
     6481 gcaagagcgc cgcgcaggtc accctgcgct ggctggtgca gcagcccggc gtggccacca
     6541 tccccaaggc cgcctcgcgc cgccacatcg agagcaacct ggccatcttc gacttcgcgc
     6601 tcagcgacgc cgagtgcgcg cgcatcgccg ccctcgagcg cggcgagcgc atcgtcgacc
     6661 ccgactgggc ggattgggcc gcctgagaca aggagcgcgg gcgtgcccat cgcgacacaa
     6721 ctggccggct tcgtcctgac catgatcggc gtcgggacgt tcctggcgac cggcgcggag
     6781 gagccgaccg cgctgatccc ggcgctgttc ggcgtggcgc tgtggggcgc cggcttcgtc
     6841 gccgagcgcg acgaggccaa gcgcatgcac gccatgcacg ccgccatgct ggtggcgctg
     6901 ctcggcgcgc tcgcgacggc ccccacggcg ctcggcgtgg gcgcgatggg cgacgcgagc
     6961 gagtcggcac gcatcgagtc gtggctgacc gtcatcgtgt gcctggtgta cctggccggc
     7021 ggcgtgcgct cgttcgtggc ggcccgccgc tcgcgctgag gcgggcggcg acgcgggcgc
     7081 ggtcatggct gcccgctgac cgagcccggc cggcatgccg gtcgcctatg ctgcaagcgt
     7141 gtgggcgcgc gcgctgagct tcctgctgtt gaccggcctc gccgcggcct gcacggccga
     7201 ggaggccatg gacagcgcgt tccagagcct ttcgcggaac ccgccgcccg gcgcggcgac
     7261 acctgagacg ggggacgagg gcgacgcggg caccgagcag cccagcgcgt gcccggacgg
     7321 cgagcgctgc ttcgggcgcc cggcccgcac cggcgccttc gacgtcgccg aggtccccga
     7381 ggccagcggc gccgcccgct cgctgcggga cccgggcgtg ctgtggctgg tcgacgaccg
     7441 gcccgggcag gcggaactcc tggcggtgcg gccggacggc tccgccgccg ggcgcctgcg
     7501 gctcgcgggc gccgagaccg tcaacgtcga ggccctggcc gcggggccgt gcgaggccgg
     7561 cggcgacgcg ccctgcctgt acatcgccga catcggcgac aacacccgct cgcgggccga
     7621 cgtgcgcgtc ctgcgcgtgc ccgagccgga cctggccggc gggccggcgc ccgccgccac
     7681 gcccgacgtc atcgcgctgc gctacccgga tcagccgcac gacgccgagg cgctgctggt
     7741 cgacgccggc ggcgtgccgt acgtgatcac caaggcgccg ttcgacgcgg ccagcggcgt
     7801 cacgggggcc accaggctgt tcgcggcacc gggcttccgc gacgggctgc tgcttgacct
     7861 gggcgcggtg ccgctgccgg agccggaggt cgctgtcgcg acgctgttct acggcaacac
     7921 ggtgacgggg gcggactccg cgcccggccg ggtgctgctg cgcacctacg accaggccgt
     7981 cgagtacgtg gcgccatcgc cggacgccga tctggcagga ttcgccgcgt ggccgcacca
     8041 gcgggtgccg gcaccgtggc agccgcaggc ggaggcggtc gcctacgccg gcgaccactg
     8101 cggctactac acgctttccg aaggcagcgg cgacatctgg ctggttccgt gcgagggcac
     8161 ggacgcgggc ggccgggacg cgccgtgaca ccgatcccga gggagaactc atgagcacga
     8221 cgtggcggcg cgccctggtc accggcgcct cgagcgggat cggcagggcg atcgcgctgg
     8281 aggcggccgc ccgcggcgcg gacgtggtgc tggtggcacg gcgggcgtcg atgctccagg
     8341 acgtggcgga cacggtccgc caggcgcacg gccgcgccgc ggagatcctg gtcgccgatc
     8401 tcaccgccca ggacgggctg gacgcggtgg aggcgcgcct gcgcgacgag tcctcaccga
     8461 tcgacctgct ggtgaacaac gccggcttcg gcacgttcgg gacgttccac cgcctgccgg
     8521 tggcacgcga ggacgaggag atccgcctca acgtgctggc cctggtgcgg ctgtcccacg
     8581 ccgctctgtc ggcgatggtg cccaggggcc acggcggcgt gctgaacgtg tcctcctcgg
     8641 cggggttcca ggccatgccc tacagcgcga cgtactgctc cacgaaggcg ttcgtgaaca
     8701 cgttcaccga gtgcctgcac gaggaggcca agggcgccgg cgtgcacgtc agcgcgctgt
     8761 gccccggcta cgtgcgcacg gagttccagg acgtcgccaa cgtctccacc tccgtgattc
     8821 cgaagttcgc ctggctggac gtgcacgccg tggcgcgcca gggcctggac gcggtgagcg
     8881 gcaacgacgc cctgtcggtg accggcggcc tgtaccgcgc cggcatcgcc ctgaaccgcc
     8941 tgctgccgcg cgcggccgtg cgccgcatcg ccggagcggt ggccagccgg cgcatgaagc
     9001 tctagacgcc ctcggccgcg cgccgcagca gcagcgggta gccggccacc accaccgcgg
     9061 cgaacaggaa ccactggatc gcgtaggaga ggtgcggccc cgcgtcctgc tccggcacgg
     9121 gccccggcac cggcagcggc cccggcgggg gctgctgctc gcgcagcagc aggaacacgt
     9181 cctccacggc ccgcgcctgc ccccccagca cggcgtccag gacggcgtcg ggatcgacgg
     9241 cgcgcacgcg cacgagccgg ccgccttcgc gcacgccctg cggtagcgcc tccgcggcgg
     9301 gcagcagcac gccctccagg cgcaccggcc cggcggccgc cgcggccccg ggcacgggcg
     9361 gcgtcgcatg gcggaacggc acccagccgc ggtccaccag cagcgccgtg ccgtcgtcca
     9421 gcagcagcgg ggtgagcacg tgatggccgg ggccgagctc gttgcgcggg gtcagcagga
     9481 cgtcggcgtc tggccggtag cgcccggaca cggtgacggg ccggtacgcg cgctcgcctt
     9541 ccggcagcga ggcgaacggg gccggctccc gcgccaggcc ctgcgcgatg cggtcgttgc
     9601 gctcggcgcg ccagtcgtga cggcgaagct gccacaggcc gaggttggcg aacgtgacga
     9661 ccaccacgac ggccagcagg tggccggcga tccacttggg cttgagcagg gtgcgcacgc
     9721 ggccatggtg cccgccggcg gcgggtccgg cgaaccggca catgcgatga tccggcggtc
     9781 acgacgggag cgacggcagg agcggcgatg aagttcggca tcacgatgtt ccccaccgac
     9841 gaggcgatgc gccccgacga gctggcgatc gccgtggagg accgcggctt cgactcgctg
     9901 tggttcccgg agcacacgca catcccggcg tcgcgcgaca ccccgtaccc gggcggtggc
     9961 gacctgccgc gcgagtacgc gcgcacgcac gatcctctgg tggcgctggc cttcgccgcg
    10021 ggcgtcaccg agcggctgct gctgggaacc ggcgtgtgcc tggtcgtgga gcgcgacccg
    10081 atcgtgctgg ccaaggaggt cgcgagcctg gacgcgctgt ccggcgggcg ggtcctgttc
    10141 ggcgtgggcg ccggctggaa ccgcgaggag atgcgcaacc acggcaccga cccggcgacg
    10201 cgtttcgagc tgctgcgcga gcgcgtcgag gcgatgaagc agatctggac ccaggacgag
    10261 gcgacctacc acggcgactt cgtgaacttc gaccgggtgt ggtcctggcc caagccggtg
    10321 cggcgccccc acccgcccat cgtgatcggc ggtgccgggg cgagcgcgct gcagcgcgtg
    10381 gtcgactacg gcgacgaatg gatgcccatc gcccgtgggt ggggcgccgg cgacctggcc
    10441 cggcgcgtcg cggagctgcg gcgcctggcg caggaggccg gccgcgggca catccccgtg
    10501 acggcgtact cggcgagctc caagccggag tccgtggagc agttcgccga ggccggcgtg
    10561 gagcggctgg tgttctgggc gccccccgcg ccggccgacc aggtgctgcc gctgctggac
    10621 cgctacgcgc cgctggtgga gcgctacgcc ggcgcctgag atcaccgcgg catatccgcg
    10681 ccaccgccgg ggcacgtcct aggcgtcatg gacctgcacc tgtcgcaccc gccacggtcg
    10741 ctggacggct tcccggagcg gcggctggag gcgggcgcgc ggctgtggac cgtgcacccc
    10801 gacgaccgct ggccgtggtc gtgcgccgcc gacggggacg gccgcttcga cctcgaggag
    10861 ccgtggggca cgtgctacct gtcgatggac ccgctgggcg cgctgatcga gtgctgctgc
    10921 cgccgcagcc ccgaggtcga cgaggacgcg ctgctccagg cgcgcctgtc ggtcatgagg
    10981 ctggaccgcg acctgcgcct ggccgacgtc gcgcacccgc gcagcttcgg ctacggcgtg
    11041 acggcggagg tgcactccat cggcgactac gccgccacgc aggcgtgggc gcgcgccttt
    11101 cgggaggccg gcttcgacgg ggtgcactac ctgctgcgcc acgacccgtc gcggtcgctg
    11161 cgcggtgtcg cgcggttcgg gccggcgggc gagcggcgcg agtggccgcg gcccgacacc
    11221 ggccggttcg gcgaggggct gctgcgcgag gccgccgagc gcttcggcat ccgctacagc
    11281 gcggcgagga cctcggcggc ggcgcgctcc ccggactcca ccgcgccgtc catgtaaccc
    11341 gaccagcgcc ggcccgtctc gctgcccgcc cagtgcagcg gcccgaccgg gcgcgccagc
    11401 gcggggccga gctgcgtcca ggcgccgggc ggcatgtggc cgccgtagca gccgcgggtc
    11461 cactcctccg ccgaccagtc acgctcgatg tagcgcagcg gctgcgccgc ctgcgggccg
    11521 aacagccccg tgaacgcgtc gaccacggcg gcgcggcgct cgtcggccgg cacgccggac
    11581 agccggatgg cgtgcgtgcc ctccaggaag cccaacagca cgccgggcgt gccggatggc
    11641 ggggagttgt cgaacaccac ctgcaccggt ccctcgtcgc tggccgcctg gccgctcagc
    11701 ccggcgtcgc gccagaacgg ccgctcgtag acggccatgc acttcaccac ggcgccgttg
    11761 ggcacgcgct gggtgagcag gtcgcgctcg gccggcagcg gcgggtcgta ggacagccgc
    11821 ccgagcagcg tcggcgggcc ggtcaggatc gcgcggcggg catgcacctc gacgccgccg
    11881 tcggcgtgca ggctgacgcc gccggggccc cactccaccc gccgcaccgg tgtggccagg
    11941 cgcacgcgcc ccgggccgag ccgctcggcc aggcgcacgg cgagcagttg cgtgccgccg
    12001 acgacgcgat cctgctgcgc gccgcccccc gtgtcgagca gggcatcgag gccgcccgcg
    12061 ctgtgcacgt agaacaggac atggagcatc gacaggctgg cggggtcggc ggcccacacg
    12121 gcggcgctga ccacccggaa gaagcgccgg ccgccgtcgg tcttgcaggt ccggcggatc
    12181 caggtctcga acgtgaggcc gtccagctcc tcggcgtccg gcgcgtccca gggccggtcc
    12241 agcggcacgg tgcgcgcgac ccgctccagc cgctgctggg ccacgccgga cgccgaggag
    12301 ctggacggcc tcacgttcga gacctggatc cgccggacct gcaagaccga cggcggccgg
    12361 cgcttcttcc
//
LOCUS       DATMKC010000318         9194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_115205, whole genome shotgun sequence.
ACCESSION   DATMKC010000318 DATMKC010000000
VERSION     DATMKC010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115205"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNU01_09375"
     CDS             <1..210
                     /locus_tag="VNU01_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32867.1"
                     /translation="GQAVFAYRLADPEIRRHRGWFLWYLLAAGLFYTELKNVIARVAQ
                     FKELMGERVWRVTPRSRPADPEPAP"
     gene            207..569
                     /locus_tag="VNU01_09380"
     CDS             207..569
                     /locus_tag="VNU01_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH32868.1"
                     /translation="MTRRILVVDDDEDIREIARLALETVGGWQVDTEPDGQAALTRAA
                     SSPPYDLILFDVMMPGMDGPTAVRRIRADGCTTPILFLTARPRDDLVDAGADGVLRKP
                     FDPMTLAEEIRARLGWPA"
     gene            566..859
                     /locus_tag="VNU01_09385"
     CDS             566..859
                     /locus_tag="VNU01_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWH32869.1"
                     /translation="MTDPELGDMVARLWERMRPVVAERLHVMGRAAEPGADADVRAAA
                     AEAAHKLAGSLGSYGYPGGSRLAARIEEALRADVPGQSVAGLVDALRREVGLG"
     gene            complement(809..2791)
                     /locus_tag="VNU01_09390"
     CDS             complement(809..2791)
                     /locus_tag="VNU01_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32870.1"
                     /translation="MAELSDESRRLEALRALGVLDTPPEERFDRIARAAADTLGTPIA
                     LITLVDDQRQWFKARVGLPLSETPRSQSFCARAIDSPDLLVVHDAREDPRFADNPLVT
                     GEPHIVFYAGAPVSAPSGERVGTVCVIGHEPRALSEVERRFLAALATWASSELAAAEV
                     NDVLTALDRIGRRLGALLDAVPEAIVLVDGEGRLRSPNPATGRLLGCPAQEVEGTHWT
                     HWLADGADARALFGVADDRRRHPRGELRLRCADGAEVPAEVTITREEVGEGMRLVIAR
                     SIKGQKDAQAALERLRRQNELILSSAHQAILGLDRAGVVHFANPSAHTLLGAPELIGC
                     RLHEIFHSRHADGSPYPWEDCPSYATLTRGEFVRVDDEVFWRTDGTSFVAEYASAPIV
                     EDGAVTGMVLTIDDVTVRKELERLKDELVGVVSHELRTPLTAIRGSLGILGGAMGADL
                     SDRAARMVDIAVRGTDRMTRLVNDLLDLDRLRSGVVDLHFASCTVGAAVDEALESVRP
                     QAEERGITIQREAGSEVVVRADHDRLLQCLVNLLANAVKFSPDGGTVRVAAWQDGGHG
                     VVRVADEGRGIPAAQLEEIFERFRQVDPSDTRRLGGSGLGLAIVRRLAELHGGRVWAE
                     SVEGKGAAFLLRLPAPAQADSAQADLAPQGVDQPGD"
     gene            complement(2887..4713)
                     /gene="typA"
                     /locus_tag="VNU01_09395"
     CDS             complement(2887..4713)
                     /gene="typA"
                     /locus_tag="VNU01_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWH32871.1"
                     /translation="MPAREDLRNVAIVAHVDHGKTTLVDAMLWQSGVFRENQDVNERV
                     MDSMDLEREKGITILAKNTAVQYGDVKINIIDTPGHADFGGEVERGLTMVDGVLLLVD
                     ASEGPLPQTRFVLRKALSAKLPIVLVVNKIDRPDARIAEVLDEVYELFLDLDATEEQI
                     NFPILYTVAKDGQASTDLDTPGTDLKPLFETLLATIPAPTHDDEHPFQALVTNLDASP
                     YVGRLALCRVRHGRIRKGQTVAWCRVNGEVEKVKITELYVTEALDRVDAEEAGPGEII
                     AVAGIPEITIGETLTDADDPRPLPAMTIDEPSLSMTIGINTSPLSGRDGKKLTARMVK
                     GRLDAELVGNVSLRVLPTERPDAWEVQGRGELQLAILVELMRREGFELTVGKPQVVTR
                     EIDGKTHEPMELVSVDVPEDYLGVVTQLFALRKGRLEQMVNHGTGWVRMEYLVPARGL
                     IGFRTEFLTETRGTGMLHHVFDRWEPWHGEIRTRPTGSLVADRAGAVTSFAMFGLQER
                     GTLFVQPGEEVYEGMIVGENSRSEEMDVNPTKEKKLTNIRSSTGDELERLVPPKVLSL
                     EQALEFIATDECVEVTPHHVRLRKVILDQQHRRRATSKAKHA"
     gene            complement(4840..7458)
                     /locus_tag="VNU01_09400"
     CDS             complement(4840..7458)
                     /locus_tag="VNU01_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWH32872.1"
                     /translation="MSRPPVSVLVVDDDVDHRRLLERCLSDAGMVVAAVASGPEALER
                     LDGIDLVLLDYRLPGMTGLETLKAIRAVDGPSVVMVTGMGSEGLVVEAMRAGAIDYLV
                     KEPHYLRSVPERIERAWRHHDLARRTAELQRLALLVTSAEDADAVFGEIVRGARALLS
                     ADACALALRHGDDLRVVAEVGERPGDMRDVVQAAQALLDRGAGTPAGSGLLLVPLPSV
                     DGRPLGALALLDEPGEERSPEDVRLVEAFASFAGLALHNLWRFQSERRMTAELAARAG
                     QQAAVARLGQRALAGADLAALFDEAAMVVQDVLGVDYVTVLELLPDGETLLCRGAAGW
                     VQEPRRRLILGRSTWPQVRRTLAGRRPVVVTDLAAAPRPDPAPLPRPEAVVSSATVAI
                     PGTPSPFGVLAADSATRRDFTPQDVDFLQSVAHVLAAAVSSTRAQRDLAHQALHDPLT
                     GLPNRVLLLDRLGQALARRGRPRSVAVLFLDVDRFKVVNDSLGHSAGDDLLGVVATRL
                     AAAVRPADTVGRFGGDEFVVVSESIDGEAGAIELAERIAEAVSRPVRLGGRELPVTVS
                     IGVVLADTDDDPESLLRDADAAMYRAKERGRSRHELFDHSMRRRVLERLETEAALRRA
                     IEQDELRLVFQPEVSLPSGRVVALEALVRWEHPQRGLMLPSDFLPLAEETGLSVPLGR
                     WVLREACRQVASSGAAAGLHLWVNLSARELTQPDLTSAVAEALAESGLEPQRLGVEIT
                     EAVMMEDIETVGRALGELRRLGLQLAIDDFGTGFSSLTYLRRFPVGVLKVDRSFVSGI
                     LDGGEDAAIVRAVVGLGHSLGLTVVAEGVEGAAQAVALVRVGCDLAQGWHFGRPAPLG
                     ELVRPAQGEEPVAAHG"
     gene            complement(7455..7898)
                     /locus_tag="VNU01_09405"
     CDS             complement(7455..7898)
                     /locus_tag="VNU01_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH32873.1"
                     /translation="MTPPPIRVLVADDNADHRFLIVRALRDANGLALEVDSVEDGEEV
                     LDLLYGRGRFVGRQRPHLVLLDLKMPRVSGMEVLERVKADPELRDIPIVVLSSSDRPE
                     DVTEAYRVGGNSYITKPSTLAGMRAGVAELSHYWTKLAVLPDADE"
     gene            complement(7895..>9194)
                     /locus_tag="VNU01_09410"
     CDS             complement(7895..>9194)
                     /locus_tag="VNU01_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32874.1"
                     /translation="ADDDDVLALPLRSGRLLLWAGPHTPYFGREELDLLARFGVVIDL
                     AWVRCELLERQRETTVRLEEAQRIARLGAWEMDLGSGAIVWSAEMYRIFDIAEGSPVT
                     LETFTERLHPEDLDVVMAEMARTIEGGPADASMDYRIVLPDGTVRTVHGQGQVQAGAD
                     GRPARLVGTAHDITDRKAAEDALNSAYERERTARLAYERANDELESFVYSVSHDLKSP
                     IISLLGYLDYLRLDYGSLLPPEAGHYLDRMTSSAGYMEALIQDLLELSRIGRVQTDPE
                     VVDLGALVADIAAELCAAHPAASVAVDPLPSVLVNPVRARQLFTNLIGNALDHAGRGD
                     VHVRVHAEPAAGGAVAVLVTDDGRGIPDAYREKVFGVFERLDSPGDPAGTGIGLAICR
                     KVMDALGGSIAVDDVPQGTRMRLEFPASAVAGARTGAVAP"
BASE COUNT         1205 a         3416 c         3348 g         1225 t
ORIGIN      
        1 ggccaggccg tcttcgcgta ccgtctcgcc gaccccgaga tccgccgcca ccgcggctgg
       61 ttcctgtggt acctgctggc cgccggcctc ttctacacgg agctgaagaa cgtgatcgcg
      121 cgcgtggcgc agttcaagga gctgatgggc gagcgcgtgt ggcgcgtgac cccgcgcagc
      181 aggcccgcgg acccggagcc cgcgccgtga cccggcgcat cctggtggtc gacgacgacg
      241 aggacatccg cgagatcgcc cggctggccc tggagaccgt ggggggctgg caggtggaca
      301 ccgagcccga cggccaggcg gcgctgacgc gcgcggcgtc gtcgccgccg tacgacctca
      361 tcctgttcga cgtcatgatg ccgggcatgg acgggccgac cgccgtgcgg cgcatccgcg
      421 ccgacggctg caccacgccg atcctgttcc tgaccgcgcg accccgggac gatctggtcg
      481 acgcgggcgc cgacggcgtg ctgcgcaagc ccttcgatcc gatgacgctg gcggaggaga
      541 tcagggcgcg cctgggttgg cccgcatgac ggacccggag ctgggcgaca tggtggcgcg
      601 gctgtgggag cggatgcgcc ccgtggtggc cgagcggctg cacgtcatgg gccgcgcggc
      661 ggagcccggc gcggacgcgg acgtgcgcgc ggcggcggcc gaggcggctc acaagctcgc
      721 cgggtcgctc ggctcctacg gctacccggg cggctcacgg ctcgcggcac ggatcgagga
      781 ggcgttgcgc gcggacgttc cgggccagtc agtcgccggg ctggtcgacg ccctgcggcg
      841 cgaggtcggc ctgggctgag tcggcctgcg ccggcgccgg cagccgcagg aggaacgccg
      901 cccctttccc ttcgacgctc tccgcccaga cccggccgcc gtgcagttcc gccaggcggc
      961 gcacgatcgc cagccccagc ccggacccgc cgagccgccg cgtgtcggag gggtccacct
     1021 ggcggaagcg ctcgaagatc tcctccagct gcgccgccgg gatgccccgc ccctcgtcgg
     1081 cgacacggac gaccccgtgg ccgccgtcct gccacgcggc gacgcgcacg gtgccgccgt
     1141 ccggcgagaa cttgaccgcg ttggccagca ggttcaccag gcactgcaac aggcgatcgt
     1201 ggtccgcccg gacgacgacc tccgatcccg cctcgcgctg gatcgtgatc ccccgctcct
     1261 cggcctgggg gcgcaccgac tccagcgcct cgtcgacggc ggcgccgacc gtgcaggagg
     1321 cgaagtgcag gtccaccacg cccgagcgca gccggtcgag gtccagcagg tcgttgacga
     1381 ggcgggtcat gcggtccgtg ccgcgcacgg cgatgtcgac catgcgggcc gcgcggtcgg
     1441 acaggtcggc gcccatggcg ccgcccagga tgcccaggga gccgcggatc gccgtgagcg
     1501 gcgtgcgcag ctcgtggctc acgaccccga ccagctcgtc cttgagccgt tcgagctcct
     1561 tgcgcacggt cacgtcgtcg atggtgagca ccatgccggt gacggcgccg tcctcgacga
     1621 tcggggcact ggcgtactcc gccacgaacg aggtcccgtc cgtgcgccag aacacctcgt
     1681 cgtcgacgcg cacgaactcg cctcgggtca gtgtcgcgta gctggggcag tcctcccacg
     1741 ggtacggcga gccgtcggcg tggcgagagt ggaagatctc gtgcaggcgg cagccgatga
     1801 gctccggcgc gcccaacagg gtgtgcgcgg acgggttggc gaagtgcacg acgccggcgc
     1861 ggtccaggcc caggatcgcc tggtgcgcgg aggacaggat cagctcgttc tggcggcgca
     1921 ggcgctccag cgccgcctgc gcatccttct gccccttgat gctgcgcgcg atcaccaggc
     1981 gcatgccctc gcccacctcc tcccgcgtga tggtgacctc ggccggcacc tcggcgccgt
     2041 cggcgcagcg caggcgcagc tcgccgcggg gatgccgccg gcggtcgtcg gccacgccga
     2101 acagggcgcg cgcgtccgcg ccgtcggcga gccagtgcgt ccagtgcgtc ccttccactt
     2161 cttgcgccgg gcagccgagc aggcggcccg tcgccggatt cggggagcgc aggcggccct
     2221 cgccgtcgac cagcacgatc gcctccggca ccgcgtcgag cagcgcgccc agccggcggc
     2281 cgatccggtc cagcgcggtg agcacgtcgt tcacctcggc cgcggccagc tccgacgacg
     2341 cccacgtggc gagcgcggcc aggaagcggc gctcgacctc cgacagcgcc cgcggctcgt
     2401 ggccgatcac acagacggtg cccacgcgtt ccccgctggg cgcgctgacg ggcgcgcccg
     2461 cgtagaacac gatgtgcggc tccccggtga ccagcgggtt gtccgcgaag cggggatcct
     2521 cccgagcgtc gtgcacgacg agcaggtccg gcgagtcgat cgcgcgagcg cagaacgact
     2581 ggctgcgcgg cgtctccgac agcggcaggc cgacacgggc cttgaaccac tggcgctggt
     2641 cgtccaccaa ggtgatcagg gcgatcggcg tcccgagggt gtcggcggcc gcgcgggcga
     2701 tccggtcgaa gcgctcctcc ggcggggtgt ccaggacgcc cagcgcccgc agcgcctcca
     2761 ggcgccggct ctcgtcggac agctccgcca tgtgcctgct ccgtcctccc ggtcgaaggc
     2821 agcttgcccc gcgagaccgg tcgcggtcca ggccctgtgc tcgccggccc gccgagcggg
     2881 gccggctcag gcgtgcttgg ccttggaggt cgcccggcgg cggtgctgct ggtcgaggat
     2941 gaccttgcgc agccgcacgt ggtgcggcgt cacctcgacg cactcgtcgg tggcgatgaa
     3001 ctccagcgcc tgctccaggc tcagcacctt cggcggcacc agccgctcca gctcgtcgcc
     3061 ggtggacgag cggatgttgg tgagcttctt ctccttggtc gggttgacgt ccatctcctc
     3121 cgaccgggag ttctcgccca cgatcatgcc ctcgtagacc tcttcgccgg gctgcacgaa
     3181 cagcgtgccg cgctcctgga ggccgaacat cgcgaagctg gtgaccgcgc cggcgcggtc
     3241 ggcgaccagc gacccggtgg gccgcgtgcg gatctcgccg tgccacggct cccagcggtc
     3301 gaagacgtgg tggagcatgc cggtgccgcg cgtctcggtg aggaactcgg tgcggaagcc
     3361 gatcaggccg cgcgccggca ccaggtactc catgcgcacc cagccggtgc cgtggttgac
     3421 catctgctcc aggcggccct tgcgcagcgc gaagagctgc gtgaccacac ccaggtagtc
     3481 ctcgggcacg tcgacggaga cgagctccat cggctcgtgc gtcttcccgt cgatctcgcg
     3541 cgtgaccacc tgcggcttgc cgacggtgag ctcgaagccc tcccgccgca tgagctccac
     3601 caggatcgcg agctgcagct cgccgcggcc ctgcacctcc caggcgtcgg ggcgctccgt
     3661 gggcagcacg cgcagcgaca cgttgccgac cagctcggcg tccagcctgc ccttcaccat
     3721 gcgcgcggtg agcttcttgc cgtcgcggcc ggacagcggc gaggtgttga tgccgatggt
     3781 catcgacagg ctgggctcgt cgatggtcat ggcgggcagc ggccgcgggt cgtcggcgtc
     3841 ggtgagcgtc tcgccgatgg tgatctccgg gatgccggcc acggcgatga tctctcccgg
     3901 cccggcttcc tctgcgtcga cgcggtcgag cgcctcggtg acgtacagct cggtgatctt
     3961 gaccttctcg acctcgccgt tgacgcggca ccacgccacc gtctggccct tgcggatgcg
     4021 gccgtggcgg acgcggcaca gcgcgaggcg gccgacgtac ggagaggcgt cgaggttggt
     4081 gaccagcgcc tggaacgggt gctcgtcgtc gtgggtgggc gcggggatcg tggcgagcag
     4141 cgtctcgaac agcggcttga ggtcggtgcc cggcgtgtcc aggtcggtgg aggcctgccc
     4201 gtccttggcg acggtgtaca ggatggggaa gttgatctgc tcctctgtgg cgtccaggtc
     4261 caggaacagc tcgtagacct cgtcgagcac ctcggcgatg cgcgcgtcgg gacggtcgat
     4321 cttgttcacc accagcacga tcggcagctt ggccgacagg gccttgcgca gcacgaaacg
     4381 ggtctgcggc agggggccct cgctcgcgtc caccagcagc agcacgccgt cgaccatggt
     4441 gagcccgcgc tcgacctcac cgccgaagtc ggcgtggccg ggcgtgtcga tgatgttgat
     4501 cttgacgtcg ccgtactgca cggcggtgtt cttcgccagg atcgtgatgc ccttctcacg
     4561 ctccaggtcc atggagtcca tgacgcgctc gttgacgtcc tggttctcgc ggaacacgcc
     4621 ggactgccac agcatggcgt ccaccagggt ggtcttgccg tggtcgacgt gggcgacgat
     4681 ggcgacgttg cgcaggtcct cgcgcgcggg catggcggta ctcctggagc agtggggccg
     4741 gccgggtggc gggcaaggga acggcggccg acgggccgcc gttgccccag tgtatcggcg
     4801 gcacgccccg gggcacgccg gggcggcggg ccgcccggct cagccgtgcg cggccaccgg
     4861 ctcctcgccc tgcgccggcc gcaccagctc gcccagcggt gcgggccggc cgaagtgcca
     4921 gccctgcgcc aggtcgcagc ccacgcgcac gagcgcgacc gcctgcgccg cgccctcgac
     4981 gccctcggcc accacggtga gaccgagcga gtgccccagt cccaccaccg cgcggacgat
     5041 ggccgcgtcc tcgccgccat ccaggatccc cgacacgaac gaccggtcga ccttgagcac
     5101 gcccacgggg aagcggcgca ggtaggtcag cgaggagaag ccggtgccga agtcgtcgat
     5161 cgcgagctgc aggcccagcc ggcgcagctc gccgagcgcg cgcccgaccg tctcgatgtc
     5221 ctccatcatc accgcctcgg tgatctccac gcccagccgt tgcggctcga ggccggactc
     5281 ggccagggcc tccgcgacgg cggaggtcag gtccggctgg gtgagctcgc gcgcggagag
     5341 gttcacccac aggtgcagcc cggccgccgc gccgctggag gcgacctggc ggcacgcctc
     5401 gcgcaggacc cagcggccca gcggcacgct cagtccggtc tcctcggcca gcggcaggaa
     5461 gtccgacggc agcatcaggc cgcgctgcgg atgctcccag cgcaccaggg cctccagggc
     5521 caccacgcgg ccgctgggca gcgacacctc cggctggaac accaggcgca gctcgtcctg
     5581 ctcgatcgcg cgccgcagcg ccgcctcggt ctccagtcgc tccagcaccc gccgccgcat
     5641 gctgtggtcg aacagctcgt ggcgcgagcg gccccgctcc ttggcgcggt acatcgccgc
     5701 gtcggcgtcg cgcagcaggg actcggggtc gtcgtcggtg tccgccagca ccacgccgat
     5761 gctcacggtg acgggcagct cgcgccctcc caggcgaacc gggcggctga ccgcttcggc
     5821 gatgcgctcg gccagttcga tcgcgccggc ctcgccgtcg atagactccg agaccaccac
     5881 gaactcgtca ccgccgaagc ggccgaccgt gtccgcgggc cgcaccgcgg cggcgagccg
     5941 ggtcgcgacg acgcccagca ggtcgtctcc ggcgctgtgc cccaggctgt cgttgaccac
     6001 tttgaagcga tcgacgtcca ggaacagcac ggccacggag cgcgggcgcc cgcggcgggc
     6061 cagcgcctgg cccaggcggt cgagcagcag cacacggttg ggcaggccgg tgagcgggtc
     6121 gtgcagcgcc tggtgcgcga ggtcgcgctg ggcacgcgtg ctggagaccg cggcggccag
     6181 gacgtgcgcg acggactgga ggaagtcgac gtcctgcggt gtgaagtccc gccgggtggc
     6241 gctgtccgcg gccagcacgc cgaagggcga cggggtgccc gggatggcca cggtggcgct
     6301 ggacacgacc gcctccgggc gcggcagcgg cgcggggtcc ggccgcggag cggcggccag
     6361 gtcggtgacc acgacgggtc gccggccggc gagcgtccgg cgcacctgcg gccacgtgga
     6421 gcgacccagg atcaggcggc ggcgcggctc ctggacccag ccggccgcgc cacggcacag
     6481 cagggtctcg ccgtccggca gcagctccag cacggtcacg tagtccacgc ccagcacgtc
     6541 ctgcaccacc atggccgcct cgtcgaacag cgcggccagg tcggcgcccg ccagggcgcg
     6601 ctggcccagg cgcgccacgg ccgcctgctg gcccgcgcgc gccgcgagct cggcggtcat
     6661 gcgccgctcg gactggaagc gccagaggtt gtgcagcgcc agccccgcga aggaggcgaa
     6721 tgcctcgacc agccgcacgt cctcgggcga gcgctcctcc cccggctcgt ccagcagcgc
     6781 cagcgcgccc agcggcctgc cgtccacgga cggcagcggc accagcagca ggcccgagcc
     6841 ggccggtgtg cccgccccgc ggtcgagcag cgcctgcgca gcctggacga cgtcgcgcat
     6901 gtcccccggc cgctccccca cctcggccac cacgcgcagg tcgtcgccgt gccgcagggc
     6961 gagcgcgcag gcgtcggccg acagcagcgc gcgcgcgccg cgcacgatct caccgaacac
     7021 cgcgtcggcg tcctcggccg aggtgaccag cagggccagc cgctggagct ccgcggtccg
     7081 ccgggcgagg tcgtggtggc gccaggcgcg ctcgatgcgc tccggcaccg agcgcaggta
     7141 gtgcggctcc ttcaccaggt agtcgatggc gcccgcgcgc atcgcctcga cgacgaggcc
     7201 ctccgacccc atgcccgtga ccatgaccac ggaggggccg tccacggcgc ggatcgcctt
     7261 cagcgtctcc aggccggtca tgcccggcag ccggtagtcc aggaggacga ggtcgatgcc
     7321 gtccaggcgc tccagcgcct cgggcccgct cgcgaccgcc gccaccacca tgcccgcgtc
     7381 gctcaggcag cgttccagca gcctgcggtg gtcgacgtcg tcgtcgacca ccagcaccga
     7441 cacgggggga cggctcactc gtccgcgtcc ggcagcaccg cgagcttggt ccagtagtgg
     7501 gacagctcgg cgacgcccgc ccgcatcccg gccagcgtgg aaggcttggt gatgtaggag
     7561 ttcccgccca cgcggtacgc ctcggtcacg tcctcgggcc ggtcggacga cgacagcacc
     7621 acgatgggga tgtccctgag ctcggggtcg gccttgaccc gttccagcac ctccatgccg
     7681 ctcaccctgg gcatcttgag gtcgagcagc accaggtgcg gccgctggcg gcccacgaag
     7741 cggccgcgcc cgtacagcag gtcgagcacc tcctcgccgt cctcgacgct gtcgacctcc
     7801 agcgcgaggc cgttcgcgtc gcgcagggcg cgcacgatca ggaagcggtg gtcggcgttg
     7861 tcgtcggcga cgagcacgcg gatcggcggt ggggtcatgg ggcgactgct cctgtgcggg
     7921 cgccggcaac ggccgacgcg gggaactcaa ggcgcatgcg ggtgccctgg ggcacgtcgt
     7981 cgacggcgat cgagccgccg agggcgtcca tgaccttgcg gcagatcgcc agcccgatgc
     8041 cggtgccggc cgggtcgcca gggctgtcca ggcgctcgaa gacgccgaag accttttcgc
     8101 ggtaggcgtc ggggatgccg cggccgtcgt cggtcaccag cacggccacg gcgccgccgg
     8161 cggccggctc ggcgtgcacg cgcacgtgga cgtcgccgcg cccggcgtgg tcgagcgcgt
     8221 tgccgatcag gttggtgaac agctggcggg cacgcacggg gttcaccagg acggagggca
     8281 gggggtcgac cgccaccgag gccgcggggt gcgcggcgca cagctccgcg gcgatgtcgg
     8341 cgaccagcgc gccgaggtcg acgacctccg ggtcggtctg cacccgcccg atgcgcgaca
     8401 gctccagcag gtcctggatc agcgcctcca tgtagccggc gctggaggtc atgcggtcca
     8461 ggtagtggcc cgcctccggc ggcagcaggc tcccgtagtc cagccgcagg tagtccaggt
     8521 agcccagcag cgagatgatc gggctcttga ggtcgtggga gacgctgtag acgaacgact
     8581 ccagctcgtc gttggcgcgc tcgtacgcca ggcgcgccgt ccgctcgcgc tcgtaggcgc
     8641 tgttcagggc gtcctcggcc gccttgcggt cggtgatgtc gtgcgcggtg ccgacgaggc
     8701 gcgccgggcg cccgtccgcg ccggcctgca cctgcccctg gccatgcacg gtgcgaacgg
     8761 tgccgtcggg cagcacgatg cggtagtcca tgctcgcgtc ggccgggccg ccctcgatcg
     8821 tgcgcgccat ctccgccatc accacgtcca ggtcctcggg gtgcaggcgc tcggtgaacg
     8881 tctccagcgt caccggcgag ccctccgcga tgtcgaagat ccggtacatc tccgccgacc
     8941 agacgatcgc ccccgagccg aggtccatct cccacgcgcc caggcgcgcg atgcgctgcg
     9001 cctcctccag gcggacggtg gtctcgcgct ggcgctccag cagctcgcag cgcacccacg
     9061 cgaggtcgat caccacgccg aagcgggcca gcaggtcgag ctcctccctg ccgaagtacg
     9121 gcgtgtgcgg ccccgcccac aacagcagcc gcccgctgcg cagcggcagc gcgagcacgt
     9181 cgtcgtcgtc ggcg
//
LOCUS       DATMKC010000319         2529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_173741, whole genome shotgun sequence.
ACCESSION   DATMKC010000319 DATMKC010000000
VERSION     DATMKC010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173741"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VNU01_09415"
     CDS             complement(<1..271)
                     /locus_tag="VNU01_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32875.1"
                     /translation="MGQAALEWVVLDVGEVLVDETRVWATWAAVLGVPAFTLMAAIGG
                     AVTRGGDHRDAFAALGLDGWRAREAEVEARYGGFQAGDLYPDALPG"
     gene            361..1383
                     /locus_tag="VNU01_09420"
     CDS             361..1383
                     /locus_tag="VNU01_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWH32876.1"
                     /translation="MVDVESLVPNGALTAFLDAELAEPGTGPVTVTKVGEGRSNLTFR
                     VERDGRAWILRRPPMGDLPETAHDMMREHRVLSGLHGTAVRVPRPVAACADTSVIGVP
                     FYLMEEIPGAVIRGAEPESYTPDDRYRIGMETVDALAELHTVDYAAAGLGDLGKPVGY
                     TARQVERWAKQWGFMKTRELEDIEVVRGWLEEHCPGEYPHAIVHGDYKLDNVVFSAEP
                     PARLLAILDWEMATLGDPLADVGYLMNFWPEPGDPTIYGLPQPTQQPGYPTKDELLAR
                     YEQRTGYAMTDLAFYRTLALWKLAILLEGLYKRFKAGKADSDWFAVLEQVVPAMGRQA
                     REWTGA"
     gene            1376..2137
                     /locus_tag="VNU01_09425"
     CDS             1376..2137
                     /locus_tag="VNU01_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWH32877.1"
                     /translation="MPEARGVEPRRGLLVDYGGVLTSSVTRSFRAFCREQGLDGELAK
                     ETFLEAYTTADGEESAIHKVEKGLITAEEFAELLAGIFSRKAGRPVPAEGLIAGLFSR
                     MELNEELFAAVAAARRAGVRTGLLSNSWGTHAQPVDPPGLVPVDALGGSDEQHRPQRL
                     DGYPRHRFGETFDAVVISGEVGMRKPDEDIYRYAAELLGLEPAGCVFVDDLDRNVEVA
                     ERLGMAGIVHRDNDETLPRLAELLGLELSLLRGAA"
     gene            complement(2112..>2529)
                     /locus_tag="VNU01_09430"
     CDS             complement(2112..>2529)
                     /locus_tag="VNU01_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32878.1"
                     /translation="RVWLDRAHPWLVRATRGALVRTPPSVYAHCWRKSVEALRLFAPD
                     AALCAVLPSVHRSAYYAGMHPHHERGMALTRALAAELDVPLVDLAAITARHLDGLNPD
                     GIHWSWAQHREVGEAMARTLLPALRRRAATPRPAEG"
BASE COUNT          347 a          914 c          951 g          317 t
ORIGIN      
        1 ggccgggcag cgcgtccggg tacaggtcgc ccgcctggaa gccgccgtag cgcgcctcca
       61 cctccgcctc gcgcgcccgc cagccgtcca ggcccagcgc ggcgaaggcg tcgcggtggt
      121 cgccgccgcg ggtcaccgcc ccgccgatcg ccgccatgag cgtgaacgcg ggaaccccca
      181 ggacggccgc ccaggtggcc cacacgcgcg tctcgtcgac cagcacctcg ccgacgtcca
      241 gcaccaccca ctccagcgcg gcttgaccca cggattcctc cgctcctacc gttccggcgg
      301 ccaggacggc ccaagatggc ccacctggag cgtagtcgcg cgcgaggtga aggagcgggt
      361 gtggtcgacg tcgagtcgct ggtgccgaac ggggcgctca cggcgttcct ggacgccgag
      421 ctcgcggagc cgggcacggg accggtcacc gtcaccaagg tgggggaggg ccgctccaac
      481 ctcaccttcc gcgtggagcg cgacggccgc gcgtggatcc tgcggcgccc gccgatgggg
      541 gacctgcccg agaccgcgca cgacatgatg cgcgagcacc gcgtgctgtc cggcctgcac
      601 ggcaccgcgg tgcgcgtgcc ccggccggtg gccgcctgcg ccgacacgtc ggtgatcggc
      661 gtgcccttct acctgatgga ggagatcccc ggcgcggtga tccggggggc ggagccggag
      721 tcctacacgc ccgacgaccg ctaccgcatc ggcatggaga cggtcgacgc gctggcggag
      781 ctgcacaccg tcgactacgc ggccgccggc ctcggcgacc tcggcaagcc ggtcggctac
      841 acggcccggc aggtggagcg ctgggccaag cagtggggct tcatgaagac ccgggagctg
      901 gaggacatcg aggtggtgcg cggctggctg gaggagcact gccccggcga gtacccgcac
      961 gcgatcgtcc acggcgacta caagctggac aacgtggtgt tctccgccga gccgcccgcc
     1021 cggctgctgg cgatcctgga ctgggagatg gccacgctcg gcgaccccct ggccgacgtc
     1081 ggctacctga tgaacttctg gccggagccg ggcgacccca ccatctacgg gctgccgcag
     1141 cccacccagc agccgggcta ccccaccaag gacgagctgc tggcccgcta cgagcagcgc
     1201 accggctacg ccatgacgga cctggcgttc taccggacgc tggcgctgtg gaagctcgcg
     1261 atcctgctgg aggggctgta caagcgcttc aaggcgggca aggccgacag cgactggttc
     1321 gcggtactgg aacaggtggt gcccgcgatg gggcgccagg cgcgggagtg gaccggtgcc
     1381 tgaggcgcgc ggagtggagc cgcggcgggg gctgctggtc gactacggcg gcgtgctcac
     1441 ctcgtcggtg acgcgcagct tccgcgcgtt ctgccgcgag cagggcctgg acggcgagct
     1501 ggccaaggag acgttcctgg aggcctacac caccgcggac ggcgaggaga gcgccatcca
     1561 caaggtggag aagggcctga tcaccgccga ggagttcgcc gagctgctcg ccggcatctt
     1621 ctcgcgcaag gccggccggc cggtgccggc ggagggcctg atcgccgggc tgttcagccg
     1681 catggagctc aacgaggagc tgttcgccgc cgtggccgcc gcgcgccggg cgggcgtgcg
     1741 cacgggcctt ctgtccaact cctggggcac gcacgcccag ccggtcgacc cgcccgggct
     1801 cgtgcccgtg gatgcgttgg gcgggtcgga cgagcagcac cggccgcagc gcctggacgg
     1861 ctacccgcgc caccgcttcg gtgagacctt cgacgccgtg gtgatctccg gcgaggtggg
     1921 catgcgcaag cccgacgagg acatctaccg ctacgccgcc gagctgctgg gcctggagcc
     1981 cgccggctgc gtgttcgtcg acgacctcga ccgcaacgtg gaggtcgccg agcgcctggg
     2041 catggccggc atcgtccacc gcgacaacga cgagacgctg ccgcggctgg cggagctgct
     2101 cggcctcgag ctcagccttc tgcggggcgc ggcgtagcgg ctcgccggcg cagcgcgggc
     2161 agcagcgtcc gggccatggc ctcgccgacc tcgcggtgct gggcccagct ccagtggatg
     2221 ccgtcggggt tcagcccgtc caggtgccgg gcggtgatcg cggcgaggtc gaccagcggc
     2281 acgtccagct ccgcggcgag cgcgcgtgtc agcgccatgc cgcgctcgtg gtgggggtgc
     2341 atgcccgcgt agtaggccga gcggtggacg ctcggcagca ccgcgcacag cgccgcgtcg
     2401 ggggcgaaca ggcgcagcgc ctcgacgctc ttgcgccagc agtgggcgta gacgctgggc
     2461 ggcgtgcgca ccagggcgcc tcgcgtggcc cgcaccagcc acgggtgggc gcggtccagc
     2521 cacacgcgc
//
LOCUS       DATMKC010000320         5490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_54767, whole genome shotgun sequence.
ACCESSION   DATMKC010000320 DATMKC010000000
VERSION     DATMKC010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54767"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1084
                     /locus_tag="VNU01_09435"
     CDS             179..1084
                     /locus_tag="VNU01_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWH32879.1"
                     /translation="MPGVEALTHDEIREALRLLGVPEPPTHRATAQRELRATLSNYGT
                     VRLALDGAPPGARDAFVRLASDGPVDVETLLGRSWWGHGMLPPPLDWLQRRALIVVAQ
                     DRLVHAIDEAVHGFHELALPLAQATARPADAEPLVVEAAGCVVIAPRPGLLDRALTAP
                     GAELRAVADTVAVSPKSPELVRAALRAAGVPLGADATVTAKPEAPALPGSIEEAIAPR
                     AVRALLARAVAEGRQLRLEYYPSSRGGAATERVVDPWDFKDDLLRGWCHLRDGERTFA
                     VDRIGKAILLPSALEKVREEAPGEG"
     gene            complement(1081..1944)
                     /locus_tag="VNU01_09440"
     CDS             complement(1081..1944)
                     /locus_tag="VNU01_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32880.1"
                     /translation="MWTTEVFETARGTVPALVAGDGEPLVCWHGFPDLPHTFAPLAER
                     LPGRRLIAPYQRGFHPDHPPAQAYFDAGTLAADAKALLDAVSPDAPVDVVGHDWGAAA
                     VYGLVTAFPDRVSRAVTMALPPTPALLGVVTQPAQMRKSFYIWFFQMAGLPEQVLNTA
                     NGGALLDFFWATWSPGAADAPHVAQVREALAHPGVVEAALGYYRALFQPAFADPELGD
                     VRGKAFAPAGVPVLLLGGDQDGCIDADILRAGGEWLPDGRVEILEGTGHFLHLEEPDR
                     VASLIQEWLGS"
     gene            2070..3530
                     /gene="ffh"
                     /locus_tag="VNU01_09445"
     CDS             2070..3530
                     /gene="ffh"
                     /locus_tag="VNU01_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWH32881.1"
                     /translation="MFDALSDRLEAIFSGLRGRGALTEQQVDEGLREIRLALLEADVN
                     FKVVKTFVSRIRERAVGTEVSQALDPGQQVIKIVHEELVRILGESSAPLELGSRTPAV
                     VMMAGLQGSGKTTATGKLAKLLKKKGRQVLLVACDLQRPAAVQQLRVLGEQVGVPVYA
                     PVESGDPVIVARQSLDEAKRLGASVVIVDTAGRTNVDEEMMRQAADIKAAVDPVETLF
                     VIDAMIGQEAVNVALAFAEAVDFSGVILSKLDGDARGGAALSVAEVTGRPIKFASVGE
                     KLDEFEAFHPDRMAGRILGMGDVMTLIEKAEEVYTADQAQKAQEKLLKAEFTFEDFLE
                     QLNQIRKMGPLGNLLGMLPGMGKALKDVEVGDEQVKPVEAIIQSMTLAERRDPKLLNG
                     KRKRRIAAGSGRTVQEINELIRSFEEMRKMMKMLGGKGALGGGMGGGMKAMRALKGQG
                     LGGLMDAAGGMGGGGMAGGAARPKNKKVPARKKKKR"
     gene            complement(3527..3871)
                     /locus_tag="VNU01_09450"
     CDS             complement(3527..3871)
                     /locus_tag="VNU01_09450"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWH32882.1"
                     /translation="MDPVPEDRFSQLVDAALDEVPDELWGRFDNVVVVVEDEHPEEPD
                     LLGLYEGIPLTERWDYAGVLPDRISIYRLPLCDMCADEAELVEEIRITVIHELAHHVG
                     IDDHQLHDLGWA"
     gene            3901..4842
                     /locus_tag="VNU01_09455"
     CDS             3901..4842
                     /locus_tag="VNU01_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HWH32883.1"
                     /translation="MTDVLLLVAGLLVLSYAADRFVLGAARLSTALRVSPVLIGAVVI
                     GFGTSAPELLVSVLATLRGAQDIAFGNVVGSNIANVLLVLGAAAAFRPLHVSGKTLRL
                     ELPLMIAAIVALAAITVDLHVYGWEGALLLAGAVGAFALIAWTALRDREARAAMEAEL
                     AEYEGGDVSAGRSALLAVAGLIGTLAGAQMLVQGALGVSRALGVPEAVIGLTVVAIGT
                     SLPELVTAVAAARRGETDLVVGNVLGSNLFNALLVAGASGVLDTVRLDPAFRWSSALM
                     VLANLFAAGMLLSGRRLGRREAAVLLAAFVGVTAFLL"
     gene            5008..>5490
                     /gene="rpsP"
                     /locus_tag="VNU01_09460"
     CDS             5008..>5490
                     /gene="rpsP"
                     /locus_tag="VNU01_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWH32884.1"
                     /translation="MAVKMRLRREGKKKQPFYRVVVADSRSPRDGRYIEDIGYYQPTH
                     EPSTISIDGDRALYWLRNGAQPSEPVLKLLRIQGIWEEFKPGDTGRDRSEANARKAAQ
                     RAAKEEAARKAEQEAATARAEKQAAAQSDAPEVEQAVEQAVEEATQPDAGAPEETGAL
                     E"
BASE COUNT          779 a         1966 c         2029 g          716 t
ORIGIN      
        1 gggcggcatc gtcgtggcga tccagcgcca gctcggcatc cccgtgaagc tcgtgggcct
       61 cggcgagggc atcgacgacc tggccgactt cgaccccgac gcgttcgtgg aggcattgtt
      121 cgccgaaggc gcccaagatg ccgagttgga cgccgaaccg ggcgcggagt agccggccgt
      181 gcccggggtc gaggcgctca cgcacgacga gatccgcgag gcgctgcgcc tgctcggcgt
      241 gccggagccg ccgacgcacc gcgccaccgc ccagcgggag ctgcgcgcca cgctgtcgaa
      301 ctacggcacc gtgcgcctgg cgctggacgg cgcgccgccc ggcgcccgtg acgcgttcgt
      361 gcgcctggcc tccgacgggc ccgtggacgt ggagaccctg ctgggccgga gctggtgggg
      421 ccacggcatg ctgccgccgc cgctggactg gctgcagcgg cgcgcgctga tcgtggtggc
      481 gcaggaccgg ctggtccacg cgatcgacga ggcggtgcac ggcttccacg agctggcgct
      541 gccgctggcc caggcgaccg cgcggcccgc cgacgccgag ccgctggtgg tggaagcggc
      601 cggctgcgtg gtgattgcgc cgcggcccgg gctgctggac cgcgcgctga ccgccccggg
      661 cgccgagctg cgcgccgtcg ccgacaccgt cgcggtctcg ccgaagtcgc cggagctggt
      721 gcgcgcggcg ctgcgcgccg cgggcgtgcc gctgggcgcc gacgcgaccg tcaccgccaa
      781 gccggaggcg cccgcgctgc ccggctccat cgaggaggcg atcgccccgc gtgcggtgcg
      841 cgccctgctg gcccgcgccg tcgccgaggg ccgccaactg cgcctggagt actacccgtc
      901 gtcccgcggc ggcgcggcga ccgagcgcgt ggtcgacccc tgggatttca aggatgatct
      961 gctgcgcggc tggtgccacc tgcgcgacgg cgagcgcacg ttcgccgtcg accgcatcgg
     1021 caaggcgatc ctgctgccca gcgccctgga gaaggtgcgc gaggaggccc caggcgaagg
     1081 ctaggagccc agccactcct ggatgagcga cgccacacga tccggctcct ccaggtgcag
     1141 gaagtgcccg gtgccctcca ggatctccac ccggccatcc ggcagccatt cgccgccggc
     1201 gcgcaggatg tccgcgtcga tgcagccgtc ctggtccccg cccagcagca gcacggggac
     1261 acccgcgggt gcgaacgcct tgccgcgcac gtcgcccagc tcggggtcgg cgaacgcggg
     1321 ctggaacagg gcgcggtagt agcccagcgc cgcctccacc acgcccggat gggcgagcgc
     1381 ctcgcgcacc tgcgcgacat gcggcgcgtc cgccgcaccg ggggaccacg tggcccagaa
     1441 gaagtccagc agcgcgccgc cgttggcggt gttcagcacc tgctcgggca gccccgccat
     1501 ctggaagaac cagatgtaga acgacttgcg catctgcgcg ggctgcgtca ccacgcccag
     1561 cagcgcgggc gtcggcggca gcgccatcgt caccgcccgg ctgacccggt cggggaaggc
     1621 ggtgaccagc ccgtagaccg ccgccgcgcc ccagtcgtgc cccacgacgt cgacgggcgc
     1681 gtccggcgag acggcgtcca gcagcgcctt tgcgtcggcg gccagcgtgc ccgcgtcgaa
     1741 gtacgcctgt gccggtggat ggtcgggatg gaagccgcgc tggtacggcg cgatcagccg
     1801 ccggccgggc agccgctcgg ccagcggcgc gaacgtatgc ggcaggtccg ggaagccgtg
     1861 ccagcacacc agcggctcgc cgtcgcccgc caccagcgcc gggaccgtgc cccgcgccgt
     1921 ctcgaacacc tcggttgtcc acatgcccgc cagcctattt caccggcggg tccgccaggc
     1981 gcggcgcccg accgtggcat gagccgtccg caagcccgcc gctacactcg gcccccaccg
     2041 caccactgac cacgaggggc tgcacaccca tgttcgacgc gctgagcgac cgcctggaag
     2101 cgatcttctc gggcctgcgc gggcgcgggg cgctgaccga gcagcaggtc gacgaggggc
     2161 tgcgcgagat ccgcctggcg ctgctcgaag ccgacgtgaa cttcaaggtc gtcaagacct
     2221 tcgtctcgcg catccgggag cgcgccgtcg ggaccgaggt cagccaggcg ctggaccccg
     2281 gccagcaggt gatcaagatc gtgcacgaag agctcgtgcg catcctgggc gagtcctccg
     2341 cgccgctgga gctgggctcc aggacgccgg ccgtggtgat gatggccggg ctgcagggct
     2401 cggggaagac cacggccacg ggcaagctcg ccaagctgct caagaagaag ggccgccagg
     2461 tcctgctggt cgcctgcgac ctgcagcgtc ccgccgccgt gcagcagctc cgcgtgctcg
     2521 gcgagcaggt cggcgtgccg gtctacgcgc ccgtggaatc cggcgatcct gtgatcgtgg
     2581 cgcgccagtc cctggacgag gccaagcgcc tgggcgccag cgtcgtcatc gtcgacaccg
     2641 ccggccgcac caacgtcgac gaggagatga tgcggcaggc cgccgacatc aaggccgccg
     2701 tcgacccggt ggagaccctg ttcgtcatcg acgcgatgat cggccaggag gccgtcaacg
     2761 tcgcgctggc gttcgccgag gccgtcgact tcagcggcgt catcctgtcc aagctggacg
     2821 gcgacgcgcg cggcggcgcc gccctgtccg tggcggaggt caccgggcgg ccgatcaagt
     2881 tcgccagcgt cggcgagaag ctcgacgagt tcgaggcgtt ccaccccgac cgcatggccg
     2941 ggcgcatcct cggcatgggc gacgtgatga cgctcatcga gaaggccgag gaggtctaca
     3001 ccgccgacca ggcccagaag gcccaggaga agctgctcaa ggcggagttc acgttcgagg
     3061 acttcctgga gcagctcaac cagatccgca agatgggccc gctgggcaac ctgctcggca
     3121 tgctgccggg catgggcaag gcgctgaagg acgtcgaggt cggcgacgag caggtcaagc
     3181 cggtcgaggc gatcatccag tccatgaccc tggccgagcg gcgcgacccg aagctgctca
     3241 acggcaagcg caagcggcgc atcgccgccg gttccggccg caccgtgcag gagatcaacg
     3301 agctgatccg cagcttcgag gagatgcgga agatgatgaa gatgctcggc ggcaagggcg
     3361 cgctcggtgg gggcatgggc ggcggcatga aggcgatgcg cgcgctgaag ggccagggac
     3421 tgggcggcct catggacgcc gccggcggca tgggcggcgg cggcatggcc ggcggcgccg
     3481 cccgcccgaa gaacaagaag gtccccgccc gcaagaagaa gaagcgctag gcccagccga
     3541 ggtcgtgcaa ctggtggtcg tcgatgccga cgtggtgcgc cagttcgtgg atcaccgtga
     3601 tgcggatctc ctcgaccagc tcggcctcgt cggcgcacat gtcgcacagc ggcagccggt
     3661 agatcgagat gcggtcgggc aggacgccgg cgtagtccca gcgctccgtc agcgggatgc
     3721 cctcgtacag gccgagcagg tccggctcct ccgggtgctc gtcctccacc acgaccacca
     3781 cgttgtcgaa acgaccccac agctcgtcgg gcacctcgtc cagcgcggcg tcgacgagtt
     3841 gcgagaatcg gtcctcgggt acagggtcca ccgctctgca gcctacggga gttcccgaac
     3901 gtgaccgatg ttctgttgct cgtcgccgga ctgctggtgc tgtcctacgc ggcggaccgc
     3961 ttcgtgctgg gtgccgccag gctgtccacc gcgctgcgcg tgtcgccggt cctgatcggg
     4021 gccgtggtca tcggcttcgg caccagcgcg ccggagctgc tggtctccgt cctggccacg
     4081 ctgcgcggcg cgcaggacat cgcgttcggc aacgtcgtcg gctccaacat cgccaacgtg
     4141 ctgctggtgc tcggtgccgc ggccgcgttc cggccgctgc acgtctccgg caagacgctg
     4201 cggctggagc tgccgctgat gatcgcggcc atcgtggcgc tggcggcgat caccgtggac
     4261 ctgcatgtct acggctggga gggcgcgctg ctgctggccg gcgccgtcgg cgcgttcgcg
     4321 ctcatcgcgt ggacggcgct gcgcgaccgc gaggccaggg ccgccatgga ggccgagctg
     4381 gccgagtacg agggtggcga cgtctcggcc ggccgttcgg cgctgctggc cgtggcgggc
     4441 ctgatcggca cgctggccgg ggcgcagatg ctggtgcagg gggcgctggg cgtgtcgcgc
     4501 gcgctcggtg tgccggaggc cgtcatcggc ctcaccgtgg tcgccatcgg caccagcctc
     4561 ccggagctcg tgaccgccgt ggccgcggcg cgccgcggcg agaccgacct cgtcgtgggc
     4621 aacgtcctgg gctccaacct gttcaacgcg ctgctggtgg cgggggcgtc cggggtgctg
     4681 gacaccgtgc gcctggaccc ggcctttcgc tggagcagcg cgctcatggt gctggcgaac
     4741 ctgttcgccg ccgggatgct gctgtcgggg cggcgcctcg ggcggcgcga ggcggccgtc
     4801 ctgctggccg cgttcgtcgg cgtgaccgcg ttcctgctgt agcccgcggg ttgctatcct
     4861 ggctgatcgg cgtggacacc cccggctcgt ccgggcgtcc gcgccccgtg cgtgcggcct
     4921 gccgaaccgc acactcgtcc acagaccgag gtgggcgcgc cggccatcag gcggcggcac
     4981 gaaggcagtt tgaaaggttc tcccagcatg gccgtcaaga tgcgcctgcg gcgcgaaggc
     5041 aagaagaagc agccgttcta ccgcgtggtc gtggccgact cccggtcacc gcgcgacggg
     5101 cgctacatcg aggacatcgg ctactaccag ccgacccacg agccctccac catctccatc
     5161 gacggcgacc gcgcgctgta ctggctgcgc aacggcgccc agcccagcga gcccgtcctg
     5221 aagctgctgc gcatccaggg catctgggag gagttcaagc cgggcgacac cggccgggac
     5281 cgctccgagg ccaacgcgcg caaggcggcg cagcgcgccg cgaaggaaga ggccgcgcgc
     5341 aaggccgagc aagaggcggc aacggcgcgc gccgagaagc aggccgccgc gcagtcagac
     5401 gccccagagg tcgagcaggc agtggagcag gccgtcgagg aggccacgca gcccgacgcc
     5461 ggagcgccgg aggagaccgg ggccctggag
//
LOCUS       DATMKC010000321        10796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_216391, whole genome shotgun sequence.
ACCESSION   DATMKC010000321 DATMKC010000000
VERSION     DATMKC010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10796
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216391"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VNU01_09465"
     CDS             <1..1352
                     /locus_tag="VNU01_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWH32885.1"
                     /translation="NVDVAPDAQDPRALLLQFDSPLLGRRVQNLVWLPSDAPSPLPVL
                     YFLHGTLRMATAPGAPGLLDDVEGAGVNVPYPLGPSAGRAPVAFVGSTMDRSRYAVVA
                     PDIGPEPWCEHCSWVDGVGGAGVPAESHLHTELMPLVEEVFGARRDRGGRAIAGRSMG
                     GGGSFIQAFRHPDRFAFAGALSGYIDMVYDPVLGPGLWAGYTRGQGYASPAQDEIGTR
                     AINPVDLAAGLVGVDIELVLTMGEGCVAMAGQGNCAEHPVTESFPTGAGQEILLRASA
                     DRWAPRLSQLGLALWYHRREGIHGPINAEEYVAEMLPRLNRLFARPVATPERFSYKSA
                     DARFSIWGYDVEVRRPNREFLHLLGARTDGRELLLAGTGEADLVTPPAFEPGRRYTVT
                     LRQDTAGDTTLTARADAAGRLAFTVPLGPPREQDERLALVEAGAFDFPKTRVTVEER"
     gene            1349..2956
                     /locus_tag="VNU01_09470"
     CDS             1349..2956
                     /locus_tag="VNU01_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWH32886.1"
                     /translation="MRRIVLAGSRVAALCLVLAALGALPSAAEEFAPECAPPGEPMTV
                     VVGRVECQRLHSEAMGAVTAFSYYVPPACDPALGRRCPVLYYTHGTGGSYREGTQPAW
                     VRALTSGPPVDPRTVADPWRYGDLSTWVPQPALDLVIVAPHGRTVPGGHGPFAGVDTG
                     WFDWNPRYAQGGDSPRYDTPPPRASEFLLDELLPYVEANLPVSGEREGRAILGYSQGG
                     FGAYINGLTRPDLFASLGMRSGGGLPLAAPADAADPALPAPPPALPGALAPVRLPGLL
                     PLVPAAVHQRVDPPLTAEVAYGWGDPVADQAWMRGANPVDLIPNARAYDHDGRQVVHL
                     KHFVNDAVPRRQSDLTNPNVFAQFYESALYPMNLYMERVFDRYGVERDFRVGPGDHSG
                     TYGVPYFREQLAEQYARLRHWDGGGRPPPRPVRFDYRTVRSAFSVWGWSFAVEREAME
                     FLNLTDVSCDGLTLRGSGSVTVTVPARCAATGPGGERTFTVDLGPSQPADEPAGMGQS
                     SAYGRVVRVTLERRGNGPAGGGTGAGR"
     gene            2953..4455
                     /locus_tag="VNU01_09475"
     CDS             2953..4455
                     /locus_tag="VNU01_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32887.1"
                     /translation="MSRPTARLSLAAVLALAAACAAPAAYDVGGSDLAGGAAAGAPGV
                     AGGQPGDAGPGVAAIGERPGTSSPVGPATPADVPAGSGPAGRAPAGGRTPVTGGAAPS
                     GGTVAIGFQVSKNVTAAFAAVGAEGEPPEERAIVGALVDWANRNGGIAGRRIVPIVHE
                     TDATSGSFSAQAQAACATFTEDNRVFAVGSSPVGGNDALLACLAQKGTPLVEQNHWLF
                     DEPYHRRYGGLLFQPAKARPERWIPAWVDGLAEQGYFEGAVVGVVRFDAPAFERLATA
                     HLLPRLARHGVEPAEHVAIRTPGGVSDFGAMAAEINNAILRFRAAGVTHVLILENAAI
                     LSFFWMPQADSSGYRPRYGMSSASTPTTVSNQAPAAQLADAVGVGWAPPVDVYDAQHP
                     GGNPIWATCMDILREAGLSTGYKSQGFYVHPHCDTVFFLRAAMALAPRFDVDGLRAGA
                     AALGSSYLSPFSHRTHLAAGRMDGAAGWRAFAFDAACACWRYHGPVRPLP"
     gene            4595..7534
                     /locus_tag="VNU01_09480"
     CDS             4595..7534
                     /locus_tag="VNU01_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWH32888.1"
                     /translation="MSLQVAGFELTGPVLLLGAVTGMTYGILAVGLVLIYRSNRIINF
                     AHGEIGTFAAAVLGVAVNRWGVPYWVAFPLAIGLGALIGAVSEAAVLRRLRRAPAIMS
                     VVATLGLAGFLLLLALVVNNQVSAGRLFPQPVGLPSFDVGPLFVSSAYSGMLLLAPVV
                     VGALTLFLRRSRYGLAIRAAAANGDAARLAGVSTTRMSTLSWAIAGGVAAFTAILVLP
                     TRGFISTEFLGPGLLMRALTAAVIARMTSLPVALAAGLGVGVVEQVLLWNDPRGGTVE
                     MVLFVIVLLTLLVQKRQAGRDEEKGSWAAVQAWAPLPESLRAVPVIRRLGAIVAAVAA
                     VAALLLPLVATNSASIILVSIMGFGLVGLSLSIVTGLSGQLSLGQFALAGVGATVSAA
                     VTLHTGSYPLGFFVAGLAAAGVSLLIGLPALRIRGLMLAVTTLGFALAAQSWLLQQSW
                     MLGDGIDPGRPIVGAFVFDTGKRYYYVALAALAFGFWLARNVWRGGLGRSLRAIRDNE
                     DGARAFTVPATRVKLQGFTTAGFLAGLGGAVYGHALSRIGAAAFPVDASVDTVAMTVL
                     GGVGVLAGPLLGALYIIGVPEFLPLDNAGLAATSLGWLALILAYPGGIAQALRPLRDR
                     VVDALARMHGVDPVAARRRDDTPPGAALVRLPEPGPRAAAPGGVLLEVRGMRKRFGGI
                     TAVDDVSLQVRPGEILGLIGPNGAGKTTLFELIGGFTRPDEGVVRFAGQDVSALGPDR
                     RADLGLVRSFQDAGLFPTLTVLETVQLSLERAQPTRLAHALSGYRGAEARKEARAREL
                     VEMLGLHAYRNRQILELSTGTRRITELACLVALDPTVLLLDEPAAGIAQRETEALGEL
                     LLRLRGELALTIVVVEHDIPLVMRLADRIVAMESGRVIADGSPQAVREDPRVIESYLG
                     GDSAAIERSGSGGGRREDATEPLVVQPAVLAGRCAATTRGGSRCSRAARDGGWCAQHA
                     AFAGSRS"
     gene            7531..8037
                     /locus_tag="VNU01_09485"
     CDS             7531..8037
                     /locus_tag="VNU01_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32889.1"
                     /translation="MTGRVPVPVLAGALALCLALFALLAAPGLAFSSDDQLRMVAPVE
                     LERVEGEVVVRWKVSDEVARLVGERRAFFAVFVDRAPIAPGESLASLADDGCRRAPAC
                     PDRAWLAERNVHVSLVSAVRLPVLPARGWGRGGTGHEATVVLMRSDGVRRGEAADSVR
                     FLVEEDGR"
     gene            8034..10289
                     /locus_tag="VNU01_09490"
     CDS             8034..10289
                     /locus_tag="VNU01_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32890.1"
                     /translation="MSAADAAELAERGLHPSGVHEMGRRLLGVPGDGDAPPFAGTVDR
                     AGLRALAGIAAAGGAALLLPYALAPLAGPVGHALGAGAGLVRGGLVLFLLVSGAAAIA
                     VPAFTGARPRRDGVALAAELGLLVVSFAAAFARTGGMLLLLLLAAGLATGAGAAVHVP
                     LLVDRFAAEVRQRVAALHAAVGLGAAGAAMALGATAVAGGAGWRTVPLAAALVAGAAA
                     VLAAPLRDPGFGRHDTDRLHAAVREGMGMGGEDGAAGQLGPNLAYGETLRRVLLVPPV
                     RTGLGAAAVSGMFALPLHLTLLGHLEAAWRVGPAGRAVLLGVMLLAAGAGVVALAERG
                     EALFRRSPSAHAGRLAALLVAGALALGLGALLPTPVLGVPLLFAGLAATAAVLPALTV
                     ALLTVVPAAMRGHAAGVLAAMSAAAGGVAGVLLLGALGSRFGTGVAVAGLVVPALAGA
                     AALRRVGPQLETELDRRVDEIVEIEDLRRVRATGGHVPLLSCRHIDFSYGLLQILFDV
                     SFTVDEGEMVALLGTNGAGKSTLLRVISGLGLPSAGTVRLQGEDVTYLDAERRVGMGI
                     AQVPGGKATFGPLSVVDNLRVFGHSAGRRRDRVDQGIEATFAAFPRLAERRNQLAATL
                     SGGEQQMLALGKALILQPRLLLIDELSLGLAPKVVGELLAMVRGINQTGTAVVLVEQS
                     VNVALSLVEHAYFMEKGQMRFDGPAGELLERPELLRSVFLQGAAQGAPALDSAGTGQD
                     SRQDRETVTGP"
     gene            10286..>10796
                     /locus_tag="VNU01_09495"
     CDS             10286..>10796
                     /locus_tag="VNU01_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32891.1"
                     /translation="MSGNGGRTRRVRSVAVFGALALGAVACGEPAALDVAQGSVSLDE
                     LFAGDSGGQAEFGEDGAAPASGTGVPGEDGGLGAGDPGTGDGGGLVPGGDPAGPGDGR
                     ATPGRRPGASGGGASPRQVPSGKGVSATEVKVGIQASKELGAGFALVGASGEAPSERR
                     IAEALVKWVN"
BASE COUNT         1121 a         4009 c         4193 g         1473 t
ORIGIN      
        1 ggaacgtcga cgtggcgccg gacgcgcagg acccccgcgc gctgctgctg cagttcgaca
       61 gccccctgct cggccgccgc gtgcagaacc tcgtgtggct gccgtccgac gcgccgtcgc
      121 cgctgccggt gctgtacttc ctgcacggca ccttgcgcat ggcgaccgcg cccggcgcgc
      181 cggggctgct ggacgacgtc gagggcgcgg gcgtcaacgt gccctacccc ctcggcccgt
      241 cggccggccg ggcgccggtc gcgttcgtcg gctccacgat ggaccgcagc cgctacgcgg
      301 tggtggcccc cgacatcgga cccgagccgt ggtgcgagca ctgctcgtgg gtggacggcg
      361 tcggcggcgc gggggtgccc gccgagtcgc acctgcacac cgagctcatg ccgctggtgg
      421 aggaggtgtt cggcgcgcgg cgcgaccgcg gcggccgcgc catcgccggg cgctccatgg
      481 gcggcggcgg ttctttcatc caggcgttcc gccaccccga ccgcttcgcc ttcgccggcg
      541 cgctgtccgg ttacatcgac atggtgtacg acccggtgct cggtcccggc ctgtgggcgg
      601 gctacacgcg cggccagggc tacgcctccc ccgcgcagga cgagatcggc acgcgggcga
      661 tcaacccggt cgacctggcc gccggcctgg tcggcgtgga catcgagctg gtgctgacga
      721 tgggggaggg ctgcgtggcc atggccgggc agggcaactg cgccgagcac cccgtcaccg
      781 agtcgttccc gacgggagcc ggccaggaga tcctgctgcg cgccagcgcc gatcgctggg
      841 ccccgcgcct cagccagctc ggcctggcgc tgtggtacca ccgccgcgag ggcatccacg
      901 ggccgatcaa cgccgaggag tacgtcgcgg agatgctgcc gcgcctgaac cggctgttcg
      961 cccgtcccgt cgccacgccg gagcgcttca gctacaagag cgccgacgcg cgcttctcca
     1021 tctggggcta cgacgtggag gtccgccggc ccaaccgcga gttcctgcac ctgctgggcg
     1081 cgcgcaccga cggacgcgag ctgctgctgg ccggcaccgg tgaggccgac ctggtcacgc
     1141 cgccggcgtt cgaacccggc aggcgctaca cggtcacgct gcgccaggac accgccggtg
     1201 acaccacgct gacggcgcgg gccgacgcgg ccggccggct ggcgttcacc gtgccgctgg
     1261 ggccgccgcg cgagcaggac gagcgcctgg ctctggtgga ggccggcgcg ttcgacttcc
     1321 cgaagacccg cgtgaccgtg gaggagcgat gaggcggatc gtccttgctg gaagccgcgt
     1381 ggccgcgctg tgcctggtgc tggcggcgct cggcgcgctg ccctcggcgg cggaggagtt
     1441 cgcgcccgag tgcgccccgc cgggcgagcc catgaccgtg gtggtcgggc gggtcgagtg
     1501 ccagcgcctg cacagcgagg cgatgggtgc cgtcaccgcg ttcagctact acgtgccgcc
     1561 ggcctgcgac ccggcgctgg ggcggcgctg cccggtcctg tactacacgc acggcaccgg
     1621 cggctcctac cgggagggca cacagcccgc gtgggtgcgc gcgctcacca gcggcccgcc
     1681 cgtggacccg cgcaccgtcg cggacccgtg gcgctacggc gacctgtcca cctgggtgcc
     1741 ccagccggcg ctggacctgg tgatcgtggc gccgcacggg cgcaccgtgc cgggcgggca
     1801 cggtcccttc gcgggcgtcg acaccggctg gttcgactgg aacccgcgct acgcgcaagg
     1861 gggcgacagc ccgcgctacg acaccccgcc gccgcgcgcc tcggagttcc tgctcgacga
     1921 gctcctgccg tacgtggagg cgaacctccc ggtgtccggc gaacgcgagg gccgcgccat
     1981 cctgggctac tcgcagggcg gcttcggcgc ctacatcaac ggcctgaccc gcccggacct
     2041 gttcgccagc ctgggcatgc gctccggcgg cggcctgccg ctggcggcgc ccgccgacgc
     2101 tgccgacccg gccctgccgg cgccgccgcc cgccctgccg ggcgcgctgg cgcccgttcg
     2161 cctgcccggg ctgctgccgc tggtgccggc cgccgtccac cagcgcgtcg acccgccgct
     2221 gaccgccgag gtggcctacg gctggggcga cccggtggcc gaccaggcgt ggatgcgcgg
     2281 cgccaacccc gtggacctca ttcccaacgc ccgcgcctac gaccacgacg ggcgccaggt
     2341 ggtgcacctg aagcacttcg tcaacgacgc ggtgccgcgg cgccagtccg acctgaccaa
     2401 ccccaacgtg ttcgcccagt tctacgagtc cgcgctgtac ccgatgaacc tgtacatgga
     2461 gcgggtgttc gaccgctacg gcgtggagcg cgacttccgg gtcgggccgg gcgaccactc
     2521 gggcacctac ggcgtgccgt acttccgcga gcagctcgcc gagcagtacg cgcggctgcg
     2581 ccactgggac ggcggcggcc ggccgccacc ccgccccgtg cgcttcgact accgcaccgt
     2641 gcgctccgcc ttctccgtgt ggggctggtc gttcgcggtg gagcgtgagg ccatggagtt
     2701 cctgaacctc accgacgtct cgtgcgacgg gctgacgctg cgcggcagcg gctcggtcac
     2761 cgtcaccgtg cccgcgcgct gcgccgccac cggtcccggc ggggagcgca ccttcaccgt
     2821 ggacctgggc ccgtcgcagc cggccgacga gccggccgga atgggccagt cctccgccta
     2881 cgggcgcgtc gtgcgggtga cgctcgagcg tcgcggcaac gggccggccg gcggtgggac
     2941 gggcgcgggc cgatgagccg gccgacggcc cgcctgagcc tggcggcggt gctcgcgctg
     3001 gcggcggcgt gcgccgcgcc ggcggcgtac gacgtcggcg ggtcggacct ggccggcggg
     3061 gcagcggcgg gcgccccggg cgtcgccggg ggtcagcccg gcgacgccgg gccgggtgtg
     3121 gcggccatcg gggagcggcc cggcacgtcg agtcccgtcg gccccgctac gccggcggac
     3181 gtgccagcgg gcagtggccc ggccggccgg gcgcccgccg gcggccggac gccggtgacg
     3241 ggcggcgcgg caccgtccgg cggcaccgtc gccatcggct tccaggtgtc caagaacgtg
     3301 actgccgcgt tcgcggccgt gggcgccgag ggcgagccgc cggaggagcg cgcgatcgtc
     3361 ggggcgctgg tcgactgggc caaccgcaac ggcggcatcg cgggccggcg catcgtgccg
     3421 atcgtgcacg agaccgacgc gacctccggc tccttcagcg cgcaggcgca ggcggcgtgc
     3481 gcgaccttca ccgaggacaa ccgggtgttc gccgtcggct ccagcccggt gggcggcaac
     3541 gacgcgctgc tggcctgcct ggcgcagaag ggcacgccgc tggtggagca gaaccactgg
     3601 ctgttcgacg agccctacca ccgccgctac ggcgggctgc tgttccagcc tgccaaggcg
     3661 cggcccgagc gctggatccc cgcttgggtc gacggcctgg ccgagcaggg ctacttcgag
     3721 ggcgcggtgg tgggcgtggt gcgcttcgac gcgccggcgt tcgagcggct ggccaccgcg
     3781 cacctgctgc cgcggctggc ccgccacggc gtcgagcccg ccgagcacgt ggccatccgc
     3841 acgccgggcg gggtgtcgga cttcggggcc atggccgcgg agatcaacaa cgccatcctg
     3901 cgcttccgcg ccgccggggt cacccacgtg ctgatcctgg agaacgcggc gatcctgtcg
     3961 ttcttctgga tgccccaggc ggacagctcc gggtaccgcc cccgctacgg catgagcagc
     4021 gccagcacgc ccaccacggt gtccaaccag gcgccggcgg cgcagctcgc cgacgccgtg
     4081 ggcgtgggct gggccccgcc cgtcgacgtc tacgacgccc agcacccggg cggcaacccg
     4141 atctgggcga cgtgcatgga catcctgcgc gaggcgggcc tgagcaccgg ctacaagagc
     4201 cagggcttct acgtgcaccc gcactgcgac accgtgttct tcctgcgcgc cgccatggcg
     4261 ctcgcgccgc gcttcgacgt cgacggcctg cgcgccggcg ccgcggcgct ggggtcgagc
     4321 tacctgtcgc cgttctcgca ccgcacgcac ctggcggcgg ggcgcatgga cggcgccgcc
     4381 ggctggcggg cgttcgcgtt cgacgcggcc tgcgcgtgct ggcgctacca cgggcccgtg
     4441 cggcccctac catgagccga tccgggagcc gatccgggca gaacggacat ccgcccttgc
     4501 gttattctag atttactgta gcctcgccgc accgcttgag tcgaggtgcg ggacggaagg
     4561 gcaggaccgt gtgcgcgctg cgtgaggggg tggaatgagc ctccaggtgg cggggttcga
     4621 gttgaccggt ccagtgctgc tgctcggggc cgtgaccggc atgacctacg ggatcctggc
     4681 cgtcggcctg gtgctcatct accggtcgaa caggatcatc aacttcgccc acggcgagat
     4741 cggcacgttc gccgccgccg tgctgggtgt cgcggtcaac cgctggggcg tgccctactg
     4801 ggtcgcgttc cccctggcga tcgggctcgg cgccctgatc ggcgcggtga gcgaggcggc
     4861 cgtgctgcgg cggctgcgcc gcgcgccggc gatcatgagc gtggtcgcca cgctgggcct
     4921 ggccggcttc ctgctgctgc tcgcgctcgt ggtcaacaac caggtcagcg ccgggcggct
     4981 gttcccccag ccggtcgggc tgccgtcctt cgacgtcggc ccgctgttcg tgagcagcgc
     5041 ctactccggc atgctgctgc tggcgccggt cgtggtcggc gccctgacgc tgttcctgcg
     5101 ccggtcgcgc tacggcctgg cgatccgcgc ggccgccgcc aacggcgacg ccgcgcgcct
     5161 ggccggcgtg tcgaccacgc gcatgtccac cctgtcgtgg gccatcgcgg gcggcgtggc
     5221 cgcgttcacc gcgatcctgg tgctgcccac gcgcggcttc atctccacgg agttcctcgg
     5281 ccccggcctg ctcatgcgcg cgttgacggc cgccgtcatc gcccgcatga ccagcctgcc
     5341 cgtggcgctg gcggctggcc tcggcgtcgg cgtggtcgag caggtgctgc tgtggaacga
     5401 ccctcgcggg ggcaccgtcg agatggtgct gttcgtgatc gtgctgctca ccctgctggt
     5461 ccagaagcgg caggccggcc gggacgagga gaagggcagc tgggcggccg tgcaggcctg
     5521 ggcgcccctg ccggagagcc tgcgcgccgt gccggtgatc cgccgcctgg gtgcgatcgt
     5581 ggcggcggtg gcggcggtgg ccgcgctgct gctgcccctg gtggccacca actccgcctc
     5641 gatcatcctg gtgtcgatca tgggcttcgg cctggtgggc ctgtcgctgt cgatcgtcac
     5701 cggcctgtcc ggccagctgt cgctgggcca gttcgcgctc gcgggcgtcg gcgcgacggt
     5761 ctccgcggcg gtcacgctgc acaccggcag ctacccgctg ggcttcttcg tcgccgggct
     5821 ggcggccgcc ggcgtgtccc tgctcatcgg cctgcccgcg ctgcgcatcc gcgggctgat
     5881 gctcgccgtc accacgctcg gtttcgcgct cgccgcccag agctggctgc tgcagcagtc
     5941 gtggatgctc ggcgacggca tcgaccctgg gcgccccatc gtgggcgcgt tcgtcttcga
     6001 caccggcaag cgctactact acgtggcgct ggcggcgctg gcgttcggtt tctggctggc
     6061 ccgcaacgtc tggcggggcg ggctggggcg cagcctgcgt gcgatccgcg acaacgagga
     6121 cggcgcgcgc gccttcacgg tgcccgccac gcgggtcaag cttcagggct tcaccaccgc
     6181 cggcttcctg gccggcctgg gcggtgcggt gtacggccac gcgctgtcgc gcatcggcgc
     6241 ggcggcgttc cccgtcgacg ccagtgtcga caccgtcgcg atgaccgtgc tcggcggcgt
     6301 cggcgtgctg gccgggccgc tgctcggcgc cctctacatc atcggcgtgc cggagttcct
     6361 gcccctggac aacgcgggcc tcgccgccac gtcgctgggc tggctcgcgc tgatcctggc
     6421 ctatcccggc ggcatcgcgc aggcgctgcg gccgctgcgc gaccgcgtgg tcgacgcgct
     6481 cgcccgcatg cacggcgtgg acccggtggc cgcccgccgg cgcgacgaca cgccacccgg
     6541 cgccgcgctg gtgcgcctgc cggagccggg cccgcgcgcc gccgcgccgg gcggcgtgct
     6601 gctggaggtg cgcggcatgc gcaagcgctt cggcggcatc accgcggtgg acgacgtgag
     6661 cctccaggtg cgtccgggcg agatcctcgg cctgatcggg cccaacggcg cgggcaagac
     6721 cacgctgttc gagctgatcg gcgggttcac caggcccgac gagggagtgg tgcgcttcgc
     6781 cggacaggac gtcagcgcgc tgggccccga ccggcgcgcg gacctcggcc tggtgcgctc
     6841 gttccaggac gccgggctgt tccccacgct gaccgtgctg gagacggtgc agctttcgct
     6901 ggagcgcgcg cagcccacgc ggctcgccca cgcgctgtcc ggctatcgcg gcgccgaggc
     6961 gcgcaaggag gctcgcgcgc gggagctcgt ggagatgctg ggcctgcacg cctaccgcaa
     7021 ccgccagatc ctggagctgt ccaccggcac gcggcgcatc accgagctgg cgtgcctggt
     7081 cgccctggac cccacggtgc tgctgctcga cgagccggcg gccggcatcg cgcagcggga
     7141 gaccgaggcg ctcggcgagc tgctgctgcg cctgcgcggc gagctggcgc tcaccatcgt
     7201 cgtggtcgag cacgacatcc cgctggtgat gcgactggcg gaccgcatcg tggcgatgga
     7261 gtccgggcgc gtcatcgccg acggctcgcc gcaggcggtg cgcgaggacc cccgggtgat
     7321 cgagagctac ctcggcggcg acagcgccgc gatcgagcgc tccggctccg ggggcggccg
     7381 ccgcgaggac gcgaccgagc cgctcgtggt gcagcccgcg gtgctcgccg gccgctgtgc
     7441 cgccaccacg cgcggcggca gccgctgcag ccgggccgcg cgcgacggcg gctggtgcgc
     7501 ccagcacgcc gcgttcgccg ggagccggtc gtgaccggcc gcgtgcccgt gcccgtcctg
     7561 gccggcgccc tggcgctgtg cctggcgctg ttcgccctgc tggcggcccc cgggctggcg
     7621 ttctcctccg acgaccagct ccgcatggtc gcgccggtgg agctggagcg cgtcgagggc
     7681 gaggtggtgg tgcgctggaa ggtctccgat gaggtcgcgc gcctggtcgg cgagcgccgc
     7741 gccttcttcg ccgtgttcgt cgaccgcgcg ccgatcgcgc cgggcgagag cctggcgtcg
     7801 ctcgccgacg acggctgccg gcgcgcaccc gcctgcccgg accgcgcctg gctggcggag
     7861 cgcaacgtgc acgtgagcct ggtcagcgcg gtgcgcctgc cggtcctgcc ggcgcgcggc
     7921 tggggccgtg gcggcacggg gcacgaggcg accgtggtgc tgatgcgctc cgacggcgtg
     7981 cgccggggcg aggccgcgga ctcggtccgc ttcctggtgg aggaggacgg cagatgagcg
     8041 ccgcggacgc cgccgagctg gccgagcgcg gcctgcaccc cagtggcgtg cacgagatgg
     8101 gacgccgcct gctcggtgtg ccgggggatg gcgacgcgcc gccgttcgcc ggcaccgtcg
     8161 accgcgcggg gctgcgggcg ctcgcgggca tcgcggccgc cggcggcgcc gcgctgctgc
     8221 tgccgtacgc gctcgcgccg ctggccggtc ccgtgggcca tgcgctcggc gcgggagcgg
     8281 gcctggtccg cggcggcctg gtgctgttcc tgctggtgtc gggcgccgcg gccatcgctg
     8341 tgccggcgtt caccggggcg cgcccgcgcc gcgacggagt cgcgctggcg gccgagctcg
     8401 gcctgctggt ggtgtcgttc gccgcggcct tcgcgcgcac cggcggcatg ttgctgctgt
     8461 tgctgctcgc cgcgggcctg gccaccggcg ccggggcggc cgtgcacgtg ccgctgctgg
     8521 tggaccgctt cgccgccgag gtgcgccagc gtgtcgccgc gctgcacgcc gccgtcggcc
     8581 tgggcgccgc gggtgccgcc atggcgctgg gagcgaccgc cgtggcgggg ggagcgggct
     8641 ggcgcaccgt gccgctggcc gccgcgctcg tcgcgggggc cgcggccgtg ctggccgcgc
     8701 cgctgcgcga cccgggtttc ggccgccacg acaccgaccg gctgcacgcc gccgtgcgcg
     8761 agggcatggg catgggcggc gaggacggcg ccgccggcca gctcggcccg aacctggcct
     8821 acggcgagac gctgcgccgc gtgctgctcg tgcccccggt gcgcacgggg ctcggggcgg
     8881 cggccgtctc cggcatgttc gcgctgcccc tgcacctgac gctgctcgga catctcgagg
     8941 ccgcgtggcg cgtcggtccc gccggccgcg cggtcctgct cggcgtgatg ctgctcgccg
     9001 ccggcgcggg cgtggtcgcg ctcgccgagc gcggcgaggc gctgttccgc cgctccccat
     9061 cggcgcacgc cgggcgcctc gccgcgctgc tggtcgccgg cgcgctggca ctcgggctcg
     9121 gcgcgctgct gcccacgccg gtcctcggtg taccgctgct gttcgcgggc ctcgccgcga
     9181 ccgccgccgt gctgcccgcg ctcacggtcg ccctgctcac cgtggtgccg gccgcgatgc
     9241 gcggccacgc cgccggcgtg ctggccgcga tgagcgcggc cgccggcggg gttgcgggcg
     9301 tgctgctgct cggcgcgctg ggcagccgct tcggcacggg cgtggccgtc gcgggcctgg
     9361 tcgtgcccgc gctcgcgggg gcggcggcgc tgcgccgcgt ggggccgcag ctggagaccg
     9421 agctggaccg gcgcgtggac gagatcgtcg agatcgagga cctgcggcgc gtgcgtgcga
     9481 ccgggggaca cgtgccgttg ctgagctgcc ggcacatcga cttctcctac gggctgctgc
     9541 agatcctgtt cgacgtcagc ttcacggtcg acgagggcga gatggtggcg ctgctcggca
     9601 ccaacggcgc cggcaagtcc acgctgctgc gcgtcatctc cggcctcggc ctgcccagcg
     9661 ccggcacggt gcggctgcag ggcgaggacg tgacctacct ggacgccgag cggcgagtgg
     9721 gcatgggcat cgcgcaggtg cccggcggca aggcgacgtt cggcccgctg agcgtggtgg
     9781 acaacctgcg ggtgttcggc cacagcgcgg ggcgccggcg cgaccgcgtc gaccagggga
     9841 tcgaggcgac cttcgccgcc ttcccgcgcc tggccgagcg ccgcaaccag ctcgccgcca
     9901 cgctgtcggg cggcgagcag cagatgctcg ccctgggcaa ggcgctgatc ctgcagccgc
     9961 gcctgctgct gatcgacgag ctgtcgctgg ggctggcgcc caaggtcgtc ggcgagctgc
    10021 tcgcgatggt gcgcgggatc aaccagacgg gcaccgcggt ggtgctggtc gagcagtcgg
    10081 tgaacgtcgc gctctcgctg gtcgaacacg cctacttcat ggagaagggc cagatgcgct
    10141 tcgacggtcc ggcgggcgaa ctgctcgagc gccccgaact gctgcgctcg gtgttcctcc
    10201 agggcgccgc ccagggcgcg ccggcgctcg actccgcagg caccgggcag gacagccgac
    10261 aggacaggga gacggtgaca gggccatgag cggcaacggc ggcaggacgc gcagggtgcg
    10321 gtcggtggcg gtgttcggcg cgctcgcgct gggtgcggtg gcgtgtggag agccggcggc
    10381 gctcgacgtg gcgcagggca gcgtgagcct ggacgagctg ttcgccggcg actccggcgg
    10441 gcaggcggag ttcggcgagg acggtgccgc gccggcgagc ggcaccggcg tgcccggcga
    10501 ggacggtggc ctcggcgccg gcgaccccgg caccggggac ggcggcgggc tggtgcccgg
    10561 cggggacccg gccggccccg gcgacggccg ggcgacaccg gggcggcggc ccggagcgtc
    10621 gggtggcggg gcgtcgccgc ggcaggtgcc gtccggcaag ggcgtgagcg ccaccgaggt
    10681 caaggtcggc atccaggcca gcaaggagct cggcgccgga ttcgccctgg tgggcgccag
    10741 cggcgaggcg cccagcgagc gccggatcgc cgaggcgctg gtgaagtggg tcaacg
//
LOCUS       DATMKC010000322         4543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_27977, whole genome shotgun sequence.
ACCESSION   DATMKC010000322 DATMKC010000000
VERSION     DATMKC010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27977"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VNU01_09500"
     CDS             complement(<1..600)
                     /locus_tag="VNU01_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH32892.1"
                     /translation="MCSSTGVGIDAALATADLHGLLDVAERALRRAKTLPLGGAVDDV
                     ELTQAVQRLEAVSRVAESHKCAVVVEIEQRAAARGQSAGSTEDLLTRVLQLTPGEARA
                     RTELAAGLQQLPDTAAAFADGRMGVGQAAVAVKKAAEVAGRDDAEVLLATIDRTASTV
                     GQTVNRTRLAREIDTAVAKTGVDVLAERERRAFQDRKLQW"
     gene            complement(674..997)
                     /locus_tag="VNU01_09505"
     CDS             complement(674..997)
                     /locus_tag="VNU01_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HWH32893.1"
                     /translation="MLTEDDVRRVALSLPAVQERLSYGTAAWHVNGKVFARMHEEARV
                     LVLWCAGEHEKRVLLTEHSPTVFTTPHYDGHASVLVRLDAVGLGLLEQLIQDAWRARA
                     PRHLL"
     gene            complement(999..1355)
                     /locus_tag="VNU01_09510"
     CDS             complement(999..1355)
                     /locus_tag="VNU01_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWH32894.1"
                     /translation="MTEAVLAVVHAHLAAFNAADLDAVLDGFAEDAVFATGDELLIGR
                     RGIGELFRQAFGPDIQATMELRSAVVQGDTAACELSERISYQGESLEFAIAAFYTVRD
                     GRLARVKVYREGSTGA"
     gene            complement(1352..2344)
                     /gene="hemB"
                     /locus_tag="VNU01_09515"
     CDS             complement(1352..2344)
                     /gene="hemB"
                     /locus_tag="VNU01_09515"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWH32895.1"
                     /translation="MNPASFPGSRPRRLRRTAAIRAAVRETTLTPSQLVAPLFVKEGV
                     DEPQPVASMPGVSQHTVESLRAEVKELRGSGVSAVLLFGVPARKDDAGSEAWNPDGIL
                     QRGIRALREDHGDELVVMADTCLDEYTSHGHCGPLRADGSVDNDAANEAYARVAVSQA
                     QAGADFISPSGMMDGMVRAIRAALDDAGFAERAAIMSYCTKYASAFYGPFRDAAECAP
                     QFGDRSGYQMDPGNVEEGVREALADMAEGADVLMVKPAVAYLDVVHRVKQATGMRLAA
                     YHVSGEYAMVHAAAERGWLDLDRAMAETLLSIRRAGADLVITYAAGWMARRLQS"
     gene            2381..2545
                     /locus_tag="VNU01_09520"
     CDS             2381..2545
                     /locus_tag="VNU01_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32896.1"
                     /translation="MDDISTPVGITIIVIAAVVLLKAAKTVVKLAMVALIVLGFYVWF
                     GMRAADALLR"
     gene            complement(2500..4080)
                     /gene="cobA"
                     /locus_tag="VNU01_09525"
     CDS             complement(2500..4080)
                     /gene="cobA"
                     /locus_tag="VNU01_09525"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWH32897.1"
                     /translation="MTIPFGGPAARHGTVYLVGGGPGDPGLVSLRAARLLSTATFVAY
                     DRLAPSAVRALCPPGAEQVYVGKLPDRHALSQDEINALLVERAKAGDAVVRFKGGDPF
                     VLGRGSEEAAHCRVHGVPFEVVPGVTSAIAAPAYAGIPVTHRAVAPGFAVVTGHEDPT
                     KGETQVDYEALARFPGTLVLLMGVGRIGEIAAALVSAGKPASTPVALVQWGTTARQRS
                     LRATLADVAQRVAETGFTSPAVTVVGDVAALPEDLAWFEDRPLHGVSVLVPRTRHQAG
                     ELSERLRALGAEPIEAPTIAIEPGREPERLGECLARMADGGFDWVALTSANGVDAVFA
                     RLDALGLDARAFARVRIAAVGPGTAEALAARGLRADLVPERFTTQGLAEALTADAAPQ
                     RALLPRADIATPVLTRALEEAGWDVTEVEAYRTVRAEALPDGVAEAVLDGEIDVLAFA
                     SSSTVRNTLELLGGPPPESVRIASIGPVTSATCRELGLRVDAEGPTHDLDGLVEAVLR
                     TRGDQRSRASAARMPNQT"
     gene            complement(4077..>4543)
                     /locus_tag="VNU01_09530"
     CDS             complement(4077..>4543)
                     /locus_tag="VNU01_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32898.1"
                     /translation="GMKRLYVPEELGGLGAPDLPLKAVPLEPGECVSAPGQGALAIEC
                     RGDDDDALAVCKLIDHTPTHRAVLAERAFLNRLGGGCLAPVGALGTLSRLGGLELIGV
                     LGDPVRRGTIRKSSKGPYDKPEELGEQLAQDVIDAGGAAILHAIAQRRERQA"
BASE COUNT          614 a         1740 c         1595 g          594 t
ORIGIN      
        1 ccattggagc ttgcggtcct gaaacgcgcg gcgttcccgc tccgccaaca catcgacacc
       61 ggttttggcg acggcggtgt cgatctcgcg ggccaggcgg gtgcggttca cggtctggcc
      121 gaccgtcgac gccgtgcggt cgatcgtggc gagcagcacc tcggcgtcgt cacggccggc
      181 cacttcggcg gccttcttga cggccacggc ggcctggccc acgcccatgc ggccgtcggc
      241 gaacgccgcc gccgtgtccg gcaactgctg cagccccgcc gcgagctcgg tccgcgccct
      301 cgcctcacca ggggtgagtt gcagcacgcg cgtcaacagg tcctcggtcg acccggcgga
      361 ctggccgcgc gccgcagcac gctgctcgat ctcgaccacc accgcgcact tgtgcgactc
      421 cgcgaccctg ctcacagctt ccaggcgctg cacggcctgc gtgagctcga catcgtcgac
      481 cgcgccgccc agcggcagcg tcttggcgcg gcgcaacgcg cgctcggcca cgtccagcag
      541 accgtgaagg tccgcggtgg ccagcgccgc gtcgatcccg actcccgtac tcgaacacat
      601 gttcgataga ttatcacgag ggcgcgactc gtcaagccgc gaatccacag cggcacaaga
      661 ccaagcgcac tcgttagagc aggtggcgtg gcgcgcgagc ccgccaggcg tcctggatca
      721 gctgctccag caggccgagg cccacggcat caaggcgcac gagcacgctg gcatgcccgt
      781 catagtgcgg cgtcgtgaac accgtggggg agtgctccgt cagcagcacg cgcttttcgt
      841 gctcgccggc gcaccacagc accagcacgc gggcctcctc gtgcatgcgg gcgaacacct
      901 tcccgttcac gtgccatgcc gctgtgccgt aagacagccg ctcctgcacg gcgggcagcg
      961 acagcgccac gcgacggaca tcgtcctcgg tgagcaccct aggcccctgt ggagccctcg
     1021 cggtagacct tcacgcgggc caggcggccg tcgcgaacgg tgtagaaggc ggcgatggcg
     1081 aactccaagg attcgccctg gtagctgatg cgctcggaca gctcgcacgc tgcggtgtcg
     1141 ccctgcacaa ccgcggagcg cagctccatc gtcgcctgga tgtccgggcc gaacgcctgg
     1201 cggaacagct cgccgatccc gcgcctgccg atcagcagct cgtcgccggt ggcgaacacg
     1261 gcgtcctcgg cgaaaccgtc caggacggcg tcgaggtcgg cggcgttgaa cgcggcgagg
     1321 tgcgcgtgca ccaccgcgag caccgcctcc gtcacgactg cagccggcgc gccatccatc
     1381 cggcggcgta ggtgatgacg aggtcggcgc ccgcccggcg gatcgacagc agggtctccg
     1441 ccatggcgcg gtcgaggtcg agccagccgc gctcggcggc ggcgtgcacc atcgcgtact
     1501 ccccggacac gtgataggcg gccagccgca tcccggtggc ctgcttcacg cggtgcacca
     1561 cgtccaggta ggccacggcg ggcttgacca tgagcacatc ggcgccttct gccatgtcgg
     1621 ccaacgcctc ccgcacaccc tcttccacgt tgccggggtc catctggtag ccgctgcggt
     1681 cgccgaactg cggcgcgcac tcggcggcgt cgcggaacgg cccgtagaac gcgctggcgt
     1741 acttggtgca gtagctcatg atcgcggcgc gctccgcgaa gccggcgtcg tccagcgccg
     1801 cgcggatggc gcgcaccatg ccgtccatca tcccgctcgg cgagatgaag tcggcgcccg
     1861 cctgcgcctg gctcaccgcg acgcgcgcgt aggcctcgtt ggccgcgtcg ttgtccacgc
     1921 tgccgtcggc gcgcagcggc ccgcagtgcc cgtgggaggt gtactcgtcc aggcaggtgt
     1981 cggccatgac caccagctcg tcgccgtggt cctcgcgcag cgccctgatc ccccgctgca
     2041 ggatgccgtc cggattccac gcctccgagc ccgcgtcgtc ctttcgcgcc ggcacgccga
     2101 acaggagcac ggccgacacg ccggacccgc gcagctcctt cacctcggcg cgcagtgact
     2161 ccaccgtgtg ctgtgacacg ccgggcatcg acgccaccgg ctgcggctcg tccacgccct
     2221 ctttcacgaa cagcggcgcg accagctgcg acggggtgag cgtggtctcg cgcacggcgg
     2281 cgcggatcgc cgcggtgcgg cgcaggcggc ggggtcgcga gccaggaaag gaagcaggat
     2341 tcattcccat gaggatagtg ctgcggtagc gtccggcgac atggacgaca tctccacccc
     2401 cgtcggcatc accatcatcg tcatcgcggc cgtcgtgctg ctgaaggcgg ccaagaccgt
     2461 ggtgaagctg gcgatggtcg cgctgatcgt gctgggcttc tacgtctggt tcggcatgcg
     2521 tgccgccgac gccctgctgc gctgatcacc ccgggtgcgg agcaccgcct cgacgaggcc
     2581 gtcgaggtcg tgcgtcggcc cttcggcgtc cacgcgcagg cccagctccc ggcacgtcgc
     2641 cgacgtcacc gggccgatcg aggcgatgcg caccgattcc gggggcgggc cgcccagcag
     2701 ctccagggtg ttgcgcaccg tggaggacga cgcgaacgcg agcacgtcaa tctccccgtc
     2761 caggaccgcc tcggcgacac cgtccggcag ggcctcggcg cgcacggtgc ggtaggcctc
     2821 cacctccgtg acgtcccagc cggcctcctc cagcgcgcgc gtcagcacgg gggtcgcgat
     2881 gtcggcgcgc gggagcagcg cccgctgcgg cgcggcgtcc gcggtgagcg cctcggccag
     2941 accctgcgtg gtgaaccgct ccgggacgag gtcggcccgc agaccccggg cggccagcgc
     3001 ctcggcggtg cccgggccga cggccgcgat ccgcacccgc gcgaacgcgc gcgcgtccag
     3061 ccccagcgcg tccaggcgcg cgaacaccgc gtcgacaccg ttggcggacg tgagcgccac
     3121 ccagtcgaag ccgccgtccg ccatgcgcgc gaggcattcg ccgaggcgct ccggctcacg
     3181 cccgggctcg atcgcgatgg tgggcgcctc gatcggctcc gcgcccaggg cgcgcagccg
     3241 ctccgacagc tcgccggcct ggtgccgggt gcgcgggacc agcacggaga ccccgtgcag
     3301 cggccggtcc tcgaaccacg ccaggtcctc cggcagcgcc gcgacatcgc ccacgacggt
     3361 caccgccgga gacgtgaagc cggtctcggc gacgcgctgc gccacgtccg cgagcgtcgc
     3421 gcgcagcgag cgctggcgcg ccgtggtgcc ccactgcacc agcgccaccg gcgtggacgc
     3481 cggcttgccg gccgagacca gggcggcggc gatctccccg atgcggccca cgcccatgag
     3541 cagcaccagc gtgccgggga agcgcgccag cgcctcgtag tccacctgcg tctcgccctt
     3601 ggtggggtcc tcgtgcccgg tcaccacggc gaagcccggc gccaccgcgc ggtgggtcac
     3661 cgggatgccg gcgtacgcgg gcgcggctat ggcggaggtc acgccgggca ccacctcgaa
     3721 cggcacgccg tgcacccggc agtgcgccgc ctcctccgag ccgcggccca gcacgaacgg
     3781 gtcgccaccc ttgaagcgca ccaccgcgtc gcccgccttg gcgcgctcca ccagcagggc
     3841 gttgatctcg tcctggctca gcgcgtgccg gtcgggcagc ttgccgacgt agacctgctc
     3901 ggcgccgggg gggcacagcg cgcgcaccgc ggagggcgcg aggcggtcgt aggccacgaa
     3961 cgtggcggtg gacagcaggc gcgccgcgcg cagcgacacc aggccagggt cgccggggcc
     4021 gccgcccacc aggtacaccg tgccgtgccg cgccgccggc ccgccgaacg ggatggtcat
     4081 gcctgccgct cccgccgctg cgcgatggcg tggaggatcg ccgcgccgcc ggcgtcgatg
     4141 acgtcctggg cgagctgctc gccgagctcc tccggcttgt cgtagggccc cttggaggac
     4201 ttgcggatgg tgccgcgccg caccgggtcg cccagcacgc cgatcagttc caggccgccg
     4261 aggcgcgaca gggtccccag ggcgccgacg ggcgccaggc agccgccgcc caggcggttg
     4321 aggaacgcgc gctcggccag caccgcgcga tgcgtgggcg tgtggtcgat gagcttgcac
     4381 accgccagcg cgtcgtcgtc gtcgccgcgg cactcgatcg ccagcgcgcc ctggccgggc
     4441 gcggagacgc actcccccgg ctccagcggc acggccttca gcggcaggtc aggcgcgccc
     4501 agcccgccga gctcctccgg cacgtacagg cgcttcatgc cgg
//
LOCUS       DATMKC010000323         4490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_88765, whole genome shotgun sequence.
ACCESSION   DATMKC010000323 DATMKC010000000
VERSION     DATMKC010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88765"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..1485
                     /gene="rfbB"
                     /locus_tag="VNU01_09535"
     CDS             478..1485
                     /gene="rfbB"
                     /locus_tag="VNU01_09535"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWH32899.1"
                     /translation="MKILVTGGAGFIGSNFIRQTLANQPDVRITNYDKLTYAGNLESL
                     KDVADDPRYSFVRGDINDAELLEEVLPGHDAVVNFAAESHVDRSITASRDFIEANVQG
                     ANTIFNVAMRLEIPKFLHISTDETYGSIPEPDSFKEGDALEPNSPYSASKASADLLAR
                     AYRVTFGYPITVTRTANNFGPYHFPEKVIPLFVSNLIDGRKVPLYGDGRNVRDWTYVL
                     DNAAAQWLVLTEGTPGEIYNVGAGNEMSNKELTYAILERFGYTGAQADEHIEFVADRP
                     GHDLRYSVDTAKVRELGWKPQHSFSEALDATIAWYRANEWWWRPLKEQGASERQGLVG
                     K"
     gene            1482..2342
                     /gene="rfbD"
                     /locus_tag="VNU01_09540"
     CDS             1482..2342
                     /gene="rfbD"
                     /locus_tag="VNU01_09540"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWH32900.1"
                     /translation="MKVLITGAGGQLGHDLVKAFADDDVVALARGELDVTDEAAVVAA
                     MRDHAPDLVVHAAAYTKVDAAEQDAEAAWRVNAIGSWWVARACKLAGAAMVYVSSDYV
                     FDGTLGRAYTEFDRVNPQGVYARSKEAGEQLVREVCPEHYIVRTSWVQGEHGGNFVKT
                     MLRLGAQRSEQGDSLTVVADQTGSPTFTADLAPAIRRLAVTGRHGTYHVTNSGHTTWH
                     EFAQAIFDFAGLDVRVEATTTAEYGAPASRPAYSVLDNLKARLTGLEPLPEWRDSLRD
                     LVGRLQSADG"
     gene            2314..3135
                     /locus_tag="VNU01_09545"
     CDS             2314..3135
                     /locus_tag="VNU01_09545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWH32901.1"
                     /translation="MAASRAPTGRSCAPPSSSSTTTPRRSCWRAWRRWARPGSARVLE
                     LDNLGFGKAANAGVAATTAEALVIANADTRFPAGSVRAMADHLRDNPRVGALGPLIRF
                     PDGRLQWSARAFPSVGQAIGHALLGMWRPDNRWTRAYRLTDWDHRSEREVDWVSGACL
                     ALRREAFDQVGGFDPSYFMFVEDVDLCARLWEAGWTVVFSPVAEVTHAVGASVGARPF
                     RMVVEHARSLNFFFTRRYAHGWRRALVPFIRAGLVGWVLAAWTWSALRGRSIGHA"
     gene            3223..>4490
                     /locus_tag="VNU01_09550"
     CDS             3223..>4490
                     /locus_tag="VNU01_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HWH32902.1"
                     /translation="MSQVRPALLRRALLVLVAALSVATLPALPAAAQSGCTPAGGQAI
                     PGPSTIPAGDFVVIGGGWGHGVGMSQYGAQGAAKLGCTAQQILQTYYQGVTIGQAPVL
                     PESVRVSLWPERPGGAPVPAICIRAHTGAPAWQPPEGDALTQPTGSLWRVDVLVDGSY
                     TIRQFDSVDGCRNAGTSGTGTEVWSGGSAGQLLRVPLDGTPIVQLTSKSTNATYPEGR
                     PYAHGTMEFRSVAGAAGATTGSTYLTLQIDTHEGRNALERYLRGLSEMPSSWEPAALQ
                     AQAITGRSYAQDKWESFKGSRSGCHCDVYDSTSDQVYAGYIKESEPGYGHRWVAAVDA
                     TAGQTMRYDGDTAVGYYSSSHGGHSESNAFVWGSNAVAYLKPVDDSRWDLASPNPLRR
                     WATALTKEQVGQAFGVGVATSVTLPEPRGAA"
BASE COUNT          677 a         1589 c         1592 g          632 t
ORIGIN      
        1 ccagagatga gcttcccaca ccggctgcga tggctcggta gatctcgcgg cggtccttgt
       61 ggtcgttgtg atcctgccgc gattccaata ccagtgtctg gacgctgcgg gggaggccgg
      121 cgacgaggtt ccataggcac cgaacgcgag attgttcgga cttgcgctgg ctgggtgtgg
      181 ggcagccagt cgttgcccgg gcacgatcac ggcagtgagc agaaacagac cgcccccgtc
      241 ggcgcgccgg cggaacgatt cgtctacgaa cgcggaagcg ggttgcacgc cggcagtctg
      301 cggtgcgcgg gcgcgggccg cctcaggcac accgcgcagg tgtggataac aaccgtgtcc
      361 gactggccgc gtccgagacg ccagcgtgtg cgaagctttc accgacaact tctgggtgtc
      421 accagacaac cagtggtgtc gtctcctgag caggcccgag caaggtgttg ccgccaaatg
      481 aagattctcg tgaccggcgg ggccgggttc atcggctcca acttcatccg ccagacgctg
      541 gccaaccagc ccgacgtgcg gatcaccaac tacgacaagc tgacctacgc gggaaacctc
      601 gagtcgctga aggacgtcgc cgacgacccg cgctacagct tcgtgcgcgg cgacatcaac
      661 gacgccgaac tgctggagga ggtgctgccc ggtcacgacg cggtggtgaa cttcgccgcg
      721 gagagtcacg tcgaccgctc catcaccgcc tcgcgcgact tcatcgaggc caacgtccag
      781 ggcgccaaca cgatcttcaa cgtggccatg cgcctggaga tcccgaagtt cctgcacatc
      841 tccacggacg agacctacgg ctcgattccc gagccggact cgttcaagga gggcgacgcg
      901 ctggagccca actcgccgta ctcggcgagc aaggcctcgg cggacctgct ggcgcgcgcc
      961 taccgggtga cgttcggcta cccgatcacc gtgacgcgca cggccaacaa cttcgggccg
     1021 taccacttcc cggagaaggt catcccgctg ttcgtcagca acctcatcga cggcaggaag
     1081 gtcccgctgt acggcgacgg gcgcaacgtg cgcgactgga cctacgtgct ggacaacgcc
     1141 gccgcgcagt ggctggtgct gacggagggc acgcccggcg agatctacaa cgtgggcgcc
     1201 ggcaacgaga tgagcaacaa ggaattgacc tacgcgatcc tggagcgctt cggctacacc
     1261 ggcgcgcagg ccgacgagca catcgagttc gtggcggacc gccccggcca cgacctgcgc
     1321 tactccgtgg acaccgcgaa ggtgcgcgag ctgggctgga agccgcagca cagcttctcc
     1381 gaggctctgg atgccacgat cgcgtggtac cgcgccaacg agtggtggtg gcggccgctc
     1441 aaggagcagg gcgcgagcga gcggcagggc ctggtcggca agtgaaggtc ctgatcaccg
     1501 gcgccggcgg ccagctcggc cacgacctcg tcaaggcgtt cgccgacgac gacgtggtgg
     1561 cgctcgcgcg cggggagctg gacgtgaccg acgaggccgc ggtggtcgcc gcgatgcgcg
     1621 accacgcccc cgacctcgtc gtgcacgccg ccgcctacac gaaggtcgac gcggccgaac
     1681 aggacgccga ggcggcgtgg cgggtcaacg ccatcggctc gtggtgggtg gcgcgcgcct
     1741 gcaagctggc cggcgccgcg atggtctacg tctccagcga ctacgtgttc gacgggacgc
     1801 tgggccgcgc ctacaccgag ttcgaccgtg tcaacccgca gggcgtgtac gcgcgcagca
     1861 aggaggcggg ggagcagctc gtgcgcgaag tctgcccaga gcactacatc gtgcgcacgt
     1921 cgtgggtgca gggcgagcac ggcggcaact tcgtgaagac catgctccgg ctgggggccc
     1981 agaggtccga gcagggggac agcctcacgg tggtggccga ccagaccggc tcgccgacgt
     2041 tcaccgccga cctggcgccg gccatccggc gcctggccgt gaccggccgc cacggcacct
     2101 accacgtgac caacagcggc cacaccacct ggcacgagtt cgcccaggcg atcttcgact
     2161 tcgccggact ggacgtgcgc gtcgaggcga cgacgaccgc cgagtacggc gcgccggcgt
     2221 cccgcccggc ctactcggtg ctcgacaacc tcaaggcgcg cctcaccggc ctggagccgc
     2281 tgccggagtg gcgcgactcg ctgcgcgacc tggttggccg cctccagagc gccgacgggt
     2341 aggtcgtgcg cgccgccgtc gtcgtcgtca accacaacac ccaggcggag ctgctggcgt
     2401 gcctggagac gctgggcgcg tccgggtagt gcgcgcgtgc tggagctgga caatctcggc
     2461 ttcggcaagg cggccaacgc gggcgtggcg gcgaccaccg ccgaggcgtt ggtgatcgcg
     2521 aacgccgaca cgcgctttcc cgccggctcg gtgcgggcca tggcggacca cctgcgcgac
     2581 aacccgcggg tcggtgcgct cggcccgctg atccgcttcc ccgacgggcg gctgcagtgg
     2641 tcggcgcgcg ccttcccgtc cgtcggccag gcgatcggcc atgcgctgct ggggatgtgg
     2701 cgcccggaca accgctggac gcgcgcgtac cgcctgacgg actgggacca ccggtcggag
     2761 cgcgaggtgg actgggtcag cggcgcgtgc ctggcgctgc ggcgcgaggc gttcgaccag
     2821 gtcggcggct tcgacccgtc ctacttcatg ttcgtggagg acgtggacct gtgcgcgcgg
     2881 ctgtgggagg ccgggtggac ggtggtgttc tcgcccgtcg ccgaggtgac gcacgcggtg
     2941 ggcgcctccg tcggcgcgcg gccgttccgc atggtggtgg agcacgcgcg ctcgctgaac
     3001 ttcttcttca cgcggcgcta cgcgcacggc tggcgccgcg cgctcgtgcc gttcatccga
     3061 gcgggcctgg tcggctgggt cctggcggca tggacgtggt cggccctcag gggccgctcc
     3121 atcggccacg cctagacccg gggtgtgtcg aaaaaatcaa tgtcaggatg actcagtcga
     3181 cacaccccgg ttttcagagg caccgacggt agggtggcgg tcatgtcgca ggttcgcccc
     3241 gccctcctcc gccgtgccct cctggtcctc gtcgccgcgc tgagcgtggc gacgctgccg
     3301 gcgctgccgg ccgccgccca gtccggctgc acgccggcgg gcgggcaggc catccccggc
     3361 ccgtccacca tcccggccgg cgacttcgtg gtcatcggcg gcggctgggg ccacggcgtc
     3421 ggcatgagcc agtacggcgc ccagggcgcc gccaagctgg gctgtaccgc ccagcagatc
     3481 ctgcagacct actaccaggg cgtcacgatc gggcaggctc cggtgctgcc cgagtccgtc
     3541 cgggtgagcc tgtggccgga gcgcccgggc ggcgcgcccg tgcccgccat ctgcatccgt
     3601 gcgcacaccg gcgcgccggc ctggcagccg ccggaaggtg acgcgctcac gcagccgacc
     3661 ggctccctgt ggcgcgtgga cgtgctggtg gacggctcct acaccatccg ccagttcgac
     3721 tcggtcgacg gctgccgcaa tgccggcacc agcggcacgg ggaccgaggt gtggtcgggt
     3781 ggctccgccg ggcagttgct gcgcgtgccc ttggacggca cgccgatcgt gcagctgacc
     3841 tccaagtcga ccaacgccac gtacccggag ggccgcccgt acgcccacgg caccatggag
     3901 ttccgctccg tcgccggggc tgcgggcgcc acgaccggct ccacgtacct gaccctgcag
     3961 atcgacacgc acgagggccg caacgcgctg gagcgctacc tgcgcggcct gtcggagatg
     4021 ccgtcgtcct gggagcccgc cgccctgcag gcgcaggcga tcaccggccg cagctacgcg
     4081 caggacaagt gggagagctt caagggcagc cgctcgggct gccactgcga cgtctacgac
     4141 tccacgtccg accaggtcta cgccggctac atcaaggagt ccgagccggg ctacgggcac
     4201 cgctgggtcg ccgcggtgga cgccacggcc gggcagacga tgcgctacga cggtgacacc
     4261 gccgtcggct actactcctc ctcccacggc ggccacagcg agtccaacgc gttcgtgtgg
     4321 ggctccaacg cggtggccta cctcaagccg gtcgacgaca gccgctggga cctcgcctca
     4381 cccaacccgc tgcggcgctg ggcgaccgcg ctcaccaagg agcaggtcgg ccaggccttc
     4441 ggagtcggcg tggccaccag cgtcacgctg ccggagccgc gcggcgcggc
//
LOCUS       DATMKC010000324         6784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_128210, whole genome shotgun sequence.
ACCESSION   DATMKC010000324 DATMKC010000000
VERSION     DATMKC010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128210"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..258)
                     /locus_tag="VNU01_09555"
     CDS             complement(55..258)
                     /locus_tag="VNU01_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32903.1"
                     /translation="MSTDATASPTFTETADEGAVRLAIASTARPSPPGRLSTALTFGW
                     RGMLKVKHVPEQLLDVTITPVPA"
     gene            complement(255..1262)
                     /locus_tag="VNU01_09560"
     CDS             complement(255..1262)
                     /locus_tag="VNU01_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWH32904.1"
                     /translation="MTTHSPVRAGLAIEATGLVKTFGDTRAVDGVDLAVPAHSVYAVL
                     GPNGAGKTTTIRMLATLLRPDGGSARVLGHDIVGEADAVRRVVSLTGQLASVDEDLSG
                     RENLVLLGRLLGLGRAAAKSRAGELLDAFGLSEAAGKLVKHYSGGMRRRLDIAASIVV
                     TPELMFLDEPTNGLDPRSRNQVWSIVRALVAEGTTILLCTQYLEEADQLADGIAVIDR
                     GKVIAEGTPAQLKASVGSGALHVRLLDPEQCPQAADLLDRALGAVNLEADPAALTATC
                     SDPERAADAVAALARAGVGVASFTLGQPSLDEVFLALTGHTAEEVATDGTAQIEEAET
                     V"
     gene            complement(1380..2366)
                     /locus_tag="VNU01_09565"
     CDS             complement(1380..2366)
                     /locus_tag="VNU01_09565"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH32905.1"
                     /translation="MSQTVTPGDTVDPGAQFEPYRRELTAFSYRMLGSAFEAEDAVQE
                     TLLRAWRGFEGFEGRASVRSWLYRIATNVCLDMARGRTRRARPMDLGPAGATATAQLV
                     ARPHEAWLEPMPERLMLGDTLDPADVAAARESVRLAFVAALQHLPARQRAVLILREVL
                     RWPATEVAELLDTTVASVNSALQRARATLADGAVAETDVWRPLDPEQQALLGRYVAAF
                     EAYDLDALTALLHADVVQTMPPYDLWLTGHDEIRRWYLGPGIGCRGSRLVPVVANGSA
                     AFGQYKPGPDGDLEPWAVQVIEIREGAIAAMNAFLDTERLFPLFGLPPRLDR"
     gene            2506..2988
                     /locus_tag="VNU01_09570"
     CDS             2506..2988
                     /locus_tag="VNU01_09570"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH32906.1"
                     /translation="MPPIIPNLWFDTEAEEAANFYVSIFPNSRIVGVTHYNEAGPRQA
                     GMVMTVEFELDGTRFVGINGGPEFTFDEAVSFQITCETQEEIDHYWERLTDGGQEVQC
                     GWLKDRYGLAWQVVPSGMEEVLADPDSERARRAMEAMLGMVKLDIGALRAAADGRSVS
                     "
     gene            2988..3317
                     /locus_tag="VNU01_09575"
     CDS             2988..3317
                     /locus_tag="VNU01_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWH32907.1"
                     /translation="MAYDEALAERVREAIAAREALSERKMFGGIAWMLHGNMAVGIVG
                     EDLMVRVGPQAYESALREPHARPMDFTGRPMNGYVFVAAPGLTADQDLAAWVARGAGF
                     AETLPPK"
     gene            complement(3324..5540)
                     /locus_tag="VNU01_09580"
     CDS             complement(3324..5540)
                     /locus_tag="VNU01_09580"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWH32908.1"
                     /translation="MKITYTHTDEAPALATYSLLPILEAFAAACGVDVETRDISLAGR
                     ILAAFPDRLREDQRVPDALAELAELAQTPQANIIKLPNISASIPQLKAAIKELQDAGY
                     AVPDYPEQADTDELRDIKARYDAVKGSAVNPVLREGNSDRRAPKSVKEYARKHPHSMG
                     AWSGESKTHVATMSSGDFRSSERSVTVEEATDVRIELTDGAGSVSVLRERVSLGAGEV
                     IDAAVMSRAALVEFLTEQIADAKRQGVLFSIHLKATMMKVSDPIIFGHAVRTFFAGLF
                     AEHGDALDAAGFDPSDGLASLDAALARLPEDQRAAVQDALRAAYEQGPALAMVDSDRG
                     ITNLHVPSDVIIDASMPAMIRTSGQMWNAAGEQQDAKAVIPDSSYAPLYDETIKFHKE
                     HGAFDPATMGTVPNVGLMAQKAEEYGSHDKTFEIPAAGTVRVVDGAGTVLLEHDVEAG
                     DIWRMCQTTDAAIRDWVKLAVTRARATGWPAVFWLDETRPHDAQLLAKVHDYLGEHDT
                     DGLDIRVMDVASAARFSLERAKRGENTISVTGNVLRDYLTDLFPILELGTSAKMLSIV
                     PLMNGGGLFETGAGGSAPKHVQQFVKENHLRWDSLGEFLALAASLEFLGEATGNPRAR
                     ILADALDAATAGVLENGRSPSRKAGELDNRGSHFYLALYWARALAEQSADAQLAERFA
                     ALAERLAANEAAIVSELNAVQGRPVDLGGYYFPDPERASAAMRPSATFNELLASLA"
     gene            complement(5681..6253)
                     /locus_tag="VNU01_09585"
     CDS             complement(5681..6253)
                     /locus_tag="VNU01_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32909.1"
                     /translation="MKTTRGLRALTGVLALALALTACGGGDEEAEPAATDSETEAADA
                     GEEIQVTGVDYAYEGAPSTAEAGTRLTFANGSENEAHEMVLVRIQDGEERPLEELLAL
                     PQEESESLVEFQGVAFAFPGEEAMYPEGETVLSEAGRYAIVCFIPVGQDIEQLRAAIA
                     AQGEDAEGPPEMGDGPPHFTQGMAAEITVS"
     gene            complement(6360..>6784)
                     /locus_tag="VNU01_09590"
     CDS             complement(6360..>6784)
                     /locus_tag="VNU01_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32910.1"
                     /translation="TVEAYDTIVWATGYRPEFPFLAGGIPWRDGAPDLHLQYASRTRD
                     DLFVLGLFETDGGAYPLLSLQAQALAAELALRREHPHVAALLRARKAARPDLRGGRQY
                     LDTARHSKYLHSRTYQRHMRDHLAYLASLAAPAGAVRT"
BASE COUNT          904 a         2415 c         2491 g          974 t
ORIGIN      
        1 ggcgcggcat gctcaaggtg aagcacgtgc cggagcagct gctggacgtg accatcacgc
       61 cggtaccggc gtgatggtca cgtccagcag ctgctccggc acgtgcttca ccttgagcat
      121 gccgcgccag ccgaacgtga gcgccgtgga caggcgcccg ggcggggacg gccgggccgt
      181 ggaggcgatc gccaggcgga cggcgccctc gtcggccgtc tcggtgaacg tgggggatgc
      241 ggtcgcgtcg gtgctcatac ggtctcggcc tcctcgatct gcgcggtccc gtcggtggcg
      301 acttcctccg cggtatggcc ggtcagcgcc aggaacacct cgtccaggct gggctgtccc
      361 agcgtgaagc tcgccacgcc gaccccggcg cgcgccagcg cggcgacggc gtcggccgcg
      421 cgctccgggt ccgagcacgt cgccgtcagg gccgccgggt ccgcctccag gttgaccgcg
      481 cccagggccc ggtccagcag gtccgccgcc tgcgggcact gctcggggtc cagcaggcgg
      541 acgtgcagcg cgccggagcc cacggaggcc ttcagctgcg cgggcgtgcc ctcggcgatg
      601 accttgccgc ggtcgatgac cgcgatgccg tcggcgagct ggtcggcctc ctccaggtac
      661 tgggtgcaca gcaggatcgt cgtgccctcg gccaccagcg cccggacgat gctccagacc
      721 tggttgcgcg agcgcgggtc cagcccgttc gtgggctcgt ccaggaacat cagctccggg
      781 gtcacgacga tgctcgccgc gatgtccagc cgccggcgca tgccgccgga gtagtgcttc
      841 accagcttgc cggcggcctc ggacaggccg aaggcgtcca gcagctcccc ggcgcgcgac
      901 ttcgccgcgg ctcgtcccag gcccagcagg cgcccgagca ggaccaggtt ctcccggccg
      961 ctcaggtcct cgtccaccga ggcgagctgg ccggtgaggc tcacgacccg gcggaccgcg
     1021 tcggcctcgc cgacgatgtc atggccgagc acgcgggcgc tgccgccatc cgggcgcagc
     1081 agcgtggcga gcatgcggat ggtggtggtc ttgccggcgc cgttggggcc gagcaccgcg
     1141 tagacggagt gcgcgggcac cgcgaggtcc acgccgtcga ccgcccgggt gtcgccgaag
     1201 gtcttcacca gcccggtggc ctcgatggcc agcccggcgc gtacggggga gtgcgtcgtc
     1261 atgcgcgatc tttccattcc gaatcgacag aacctgtccg gggcacgtgc ctcggacgca
     1321 ttcttgcgcg gccctgtgac agtgccgacg acagtagtga cgtcagcgcc gccgggaact
     1381 catcggtcca gtcgtggcgg cagcccgaac agcgggaaca gccgctcggt gtccaggaac
     1441 gcgttcatcg ccgcgatcgc gccctcgcgg atctcgatca cctgcaccgc ccacggctcc
     1501 aggtccccgt ccggccccgg cttgtactgc ccgaacgccg ccgagccgtt cgccaccacc
     1561 gggaccaggc gcgagccccg gcagccgatg cccgggccga ggtaccagcg gcggatctcg
     1621 tcgtggccgg tgagccacag gtcgtagggc ggcatcgtct gcacgacatc ggcgtgcagc
     1681 agggccgtga gcgcgtccag gtcgtaggcc tcgaacgccg ccacgtagcg gcccagcagc
     1741 gcctgctgtt cggggtccag gggccgccac acgtcggtct ccgcgacggc gccgtcggcc
     1801 agcgtcgcgc gcgcccgctg cagcgcgctg ttgacggagg cgacggtggt gtcgagcagc
     1861 tcggcgacct cggtggccgg ccagcgcagc acctcgcgca ggatcagcac ggcgcgctgc
     1921 cgggcgggca ggtgctgcag cgccgccacg aacgccagcc gcaccgactc gcgtgccgcg
     1981 gcgacgtccg cgggatccag ggtgtcgccc agcatcagcc gctccggcat cggctccagc
     2041 cacgcctcgt gcgggcgcgc cacgagctgc gcggtggcgg tcgcgcccgc gggacccagg
     2101 tccatcgggc gggctcgccg ggtgcggccg cgcgccatgt ccaggcacac gttcgtggcg
     2161 atgcggtaca gccaggaccg caccgaggcg cggccctcga agccctcgaa gccgcgccag
     2221 gcgcgcagca gcgtctcctg cacggcgtcc tcggcctcga acgccgagcc gagcatgcgg
     2281 tagctgaagg cggtcagctc gcggcggtac ggttcgaact gggcgccggg gtccacggtg
     2341 tcgcccgggg tgacggtctg ggacatttgg gcagcatccc aggaaccatt gcaccggaca
     2401 agctcacgcg ggaggattcg cgcgtcgacc gatgagtccg gcctccgcgg tgcgtcctac
     2461 caccagcgca ccgcacgcat cgacgaccga agggaacgac cgctcatgcc gccgatcatc
     2521 cccaacctct ggttcgacac cgaggcggag gaggccgcga acttctatgt ctccatcttc
     2581 cccaactcgc gcatcgttgg cgtcacgcac tacaacgagg ccggcccgcg gcaggcgggc
     2641 atggtgatga ccgtggagtt cgagctggac ggcacgcgct tcgtggggat caacggcggc
     2701 ccggagttca ccttcgacga ggcggtctcc ttccagatca cctgcgagac ccaggaggag
     2761 atcgaccact actgggagcg cttgaccgac gggggccagg aggtgcagtg cggctggctc
     2821 aaggaccgct acgggctggc ctggcaggtc gtgcccagcg gcatggagga ggtcctggcc
     2881 gatccggact ccgagcgggc ccgccgggcc atggaggcga tgctgggcat ggtgaagctg
     2941 gacatcggcg cgctgcgcgc cgccgccgac ggacggtccg tgtcctgatg gcctacgacg
     3001 aggcgctggc ggagcgcgtc cgtgaggcga tcgcggcgcg cgaggcgctc agcgagcgca
     3061 agatgttcgg cgggatcgcg tggatgctgc acggcaacat ggccgtgggc atcgtcggcg
     3121 aggacctcat ggtgcgtgtg gggccgcagg cgtacgagtc cgcgctgcgg gagccgcacg
     3181 cccggcccat ggacttcacc ggccggccga tgaacggcta cgtcttcgtt gcggcgccgg
     3241 gcctcacggc cgaccaggac ctggccgcct gggtcgcccg cggcgccggc ttcgccgaga
     3301 cgctgccgcc gaagtgagcg gccctacgcc aaggaggcga gcagctcgtt gaaggtcgcg
     3361 ctgggccgca tggcggcgga ggcgcgctcg gggtcgggga agtagtagcc gcccaggtcg
     3421 accggccggc cctggacggc gttcagctcg gacacgatgg cggcctcgtt cgcggccagc
     3481 cgctccgcca gagccgcgaa gcgctcggcg agctgggcgt cggcggactg ctcggccagc
     3541 gcgcgcgccc agtacagcgc caggtagaag tggctgccgc ggttgtccag ctcgccggcc
     3601 ttgcgcgacg gcgacctgcc gttctccagc acccccgcgg tcgcggcgtc cagcgcgtcg
     3661 gccaggatgc gggcgcgcgg gttgccggtg gcctcgccca ggaactccag cgacgcggcc
     3721 agggccagga actcgcccag ggagtcccag cgcaggtggt tctccttcac gaactgctgc
     3781 acgtgcttgg gcgcggagcc gcccgccccc gtctcgaaca ggccgccgcc gttcatcagc
     3841 ggcacgatgg acagcatctt cgcgctggtg cccagctcca ggatcgggaa cagatccgta
     3901 aggtagtcgc gcagcacgtt gcccgtcacg gagatggtgt tctcgccgcg cttggcgcgc
     3961 tccaggctga accgggccgc ggaggccacg tccatcacgc ggatgtccag gccgtccgtg
     4021 tcgtgctcgc ccaggtagtc gtgcaccttg gcgagcagct gcgcgtcgtg cgggcgcgtc
     4081 tcgtccagcc agaacaccgc cggccagccc gtggcgcggg cacgcgtcac cgcgagcttc
     4141 acccagtcgc ggatcgccgc gtcggtggtc tggcacatgc gccagatgtc gccggcctcg
     4201 acgtcgtgct ccagcagcac ggtgccggcg ccgtccacca cgcgcaccgt gccggcggcg
     4261 gggatctcga aggtcttgtc gtgcgagccg tactcctcgg ccttctgcgc catgagcccg
     4321 acgttgggca cggtgcccat ggtggcgggg tcgaaggcgc cgtgctcctt gtggaacttg
     4381 atggtctcgt cgtacagcgg cgcgtagctg gagtcgggga tgaccgcctt ggcgtcctgc
     4441 tgctcgccgg cggcgttcca catctgcccg gacgtgcgga tcatcgcggg catggacgcg
     4501 tcgatgatga cgtcgctcgg cacgtgcagg ttcgtgatgc cgcggtcgga gtcgaccatc
     4561 gccagggcgg ggccctgctc gtacgccgcg cgcagggcgt cctgcaccgc cgcgcgctgg
     4621 tcctccggca gccgcgcgag cgcggcgtcc agggacgcga gcccgtcgga ggggtcgaag
     4681 cccgcggcgt ccagcgcgtc gccgtgctcg gcgaacaggc cggcgaagaa cgtgcgcacc
     4741 gcgtggccga agatgatcgg gtcggagacc ttcatcatcg tggccttgag gtggatggag
     4801 aacaggacgc cctggcgctt ggcgtcggcg atctgctccg tgaggaactc caccagcgcg
     4861 gcgcggctca tcacggcggc gtcgatcacc tcgccggccc ccagggacac ccgctcccgc
     4921 aggacgctga cggagccggc gccgtcggtc agctcgatgc gcacgtcggt cgcctcctcg
     4981 accgtcacgg accgctcgga ggagcggaag tcgccggagc tcatggtcgc cacgtgggtc
     5041 ttggactcgc ccgaccacgc gcccatggag tgcgggtgct tgcgcgcgta ctccttcacg
     5101 gacttcggcg cgcggcggtc ggagttgccc tcgcgcagca ccgggttgac cgcgctgccc
     5161 ttcacggcgt cgtagcgcgc cttgatgtcg cgcagctcgt cggtgtcggc ctgttcgggg
     5221 tagtcgggca cggcgtagcc ggcgtcctgc aactccttga tggccgcctt cagctgcggg
     5281 atcgacgcgc tgatgttggg cagcttgatg atgttggcct ggggcgtctg ggccagctcg
     5341 gccagctccg caagggcgtc ggggacgcgc tggtcctcgc gcaggcggtc cgggaaggcg
     5401 gccaggatgc gccccgcgag cgagatgtcg cgagtctcca cgtccacgcc gcaagccgcg
     5461 gcgaacgcct ccaggatggg caggagcgag tacgtcgcca gggcgggggc ctcgtcggtg
     5521 tgggtgtagg tgatcttcat ctcgcgcgcc ttgggaggtg gggccgggct ggtcagcgaa
     5581 ggctaccgga ccgtcgtgat gccccaaacc ggcggaagcc atgggggaag cggaagggcc
     5641 cgcgccgagg cgcgggccct tcactgcgat tggcatcgcg tcaggagacg gtgatctccg
     5701 cggccatgcc ctgggtgaag tgcggcggac cgtcgcccat ctccggcggg ccctccgcgt
     5761 cctcgccctg cgccgcgatg gcggcgcgga gctgctcgat gtcctggccc accgggatga
     5821 agcagacgat ggcgtagcgg ccggcctcgc tcagcaccgt ctcgccctcc gggtacatcg
     5881 cctcctcgcc ggggaaggcg aacgcgacgc cctggaactc caccagcgac tccgactcct
     5941 cctgcggcag cgcgagcagc tcctccaggg gtcgctcctc gccgtcctgg atccggacga
     6001 gcaccatctc gtgcgcctcg ttctccgacc cgttggcgaa cgtcagcctg gtgccggcct
     6061 cggccgtcga cggcgcgccc tcgtaggcgt agtcgacgcc ggtgacctgg atctcctcgc
     6121 ccgcgtcggc ggcttcggtc tcgctgtccg tcgcggccgg ctcggcctcc tcgtcaccgc
     6181 cgccgcacgc cgtcagcgcc agcgccaacg cgagcacgcc cgtgagggcc cgcaggcccc
     6241 gtgtggtctt caatgccgtc tccctcgtcg atggacgccg ctgagagcca acgacgtcga
     6301 gaggagacat gaaacacgcg cggggacctg ccgccgcgca agctgtcctg gtcgtgcggt
     6361 caggtgcgga ccgcgccggc cggggcggcc agcgaggcaa ggtaggcgag gtgatcgcgc
     6421 atgtgccgct ggtaggtccg gctgtgcagg tacttgctgt gccgggccgt gtccaggtac
     6481 tggcgcccgc cccgcaggtc cggccgcgcg gctttgcgtg cccgcagcag cgcggcgacg
     6541 tgggggtgct cccgccgcag cgccagttcg gcggccagcg cctgcgcctg cagcgacagc
     6601 aggggatagg caccgccgtc ggtctcgaac agccccagca cgaacaggtc gtcgcgtgtc
     6661 cgtgacgcgt actgcaggtg caggtccggc gcgccgtcgc gccaggggat cccgccggcc
     6721 aggaagggga actccggccg gtagcccgtc gcccagacga tcgtgtcgta ggcctccacg
     6781 gtgc
//
LOCUS       DATMKC010000325        13512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_294085, whole genome shotgun sequence.
ACCESSION   DATMKC010000325 DATMKC010000000
VERSION     DATMKC010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294085"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VNU01_09595"
     CDS             <1..141
                     /locus_tag="VNU01_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWH32911.1"
                     /translation="EMVMPGDNTEMVVELIQPIAMEDGLRFAIREGGRTVGAGRVTKI
                     LK"
     gene            447..611
                     /gene="rpmG"
                     /locus_tag="VNU01_09600"
     CDS             447..611
                     /gene="rpmG"
                     /locus_tag="VNU01_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWH32912.1"
                     /translation="MPKSDVRPKITLACTECKERNYITTKNRNTQRERVELKKYCPRC
                     KTHQPHRETK"
     gene            748..1539
                     /locus_tag="VNU01_09605"
     CDS             748..1539
                     /locus_tag="VNU01_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH32913.1"
                     /translation="MGRLQGRVAVITGAARGIGEATARLFAAEGATLALNDVDEEPLR
                     ELAASLDAVAVPGDVARDAERIVAEALAAHGAIDIVVNNAGTRADAPFHLMTAEQWDA
                     VFDACLTGTLRMCQAVAPHFRERAMAELAEHGAVAHHRKITNTAPASFLVGSPGQANL
                     TAAGGAVVGLTRTLARELGGFAVNVNAVAPGFVETRLTAAEHPDGGAGMAEPLRQMTK
                     AMTALGRHGQPGDLARVHLFLASSDADFVTGVTIPVAGGLLGTCV"
     gene            1493..2008
                     /locus_tag="VNU01_09610"
     CDS             1493..2008
                     /locus_tag="VNU01_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWH32914.1"
                     /translation="MSRSPSQGACWGRACEGRRVIALNKARVGHRYPSYRYEVSREKI
                     REYANATGVSDPQYAADEGDVVAPPMFAACFTVIRGGAGMFGDAELGAHFALVHGSQE
                     YEFHRPVRLGDVLECTPFIADIQWKGRNEFLTLQVDCNDATTGEPVVTSRGVIVFLGS
                     APQNPEQQEAS"
     gene            2009..2428
                     /locus_tag="VNU01_09615"
     CDS             2009..2428
                     /locus_tag="VNU01_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWH32915.1"
                     /translation="MPKFDEVKVGDQIPGESKTIDQMQLIQYAGASGDFNPLHWQEEF
                     AGKVSPTGGIIAHGMYNAGLMSMVVTRWAGGPENVKRIKTSFRAPCPLGATVSFGGEV
                     TALDEAARTATLAIWAELEDGSKVIDRGSSEAVVQLD"
     gene            2541..2613
                     /locus_tag="VNU01_09620"
     tRNA            2541..2613
                     /locus_tag="VNU01_09620"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2574..2576,aa:Trp,seq:cca)
     gene            2658..2924
                     /gene="secE"
                     /locus_tag="VNU01_09625"
     CDS             2658..2924
                     /gene="secE"
                     /locus_tag="VNU01_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWH32916.1"
                     /translation="MSRETQREMDEERRDERPAAPSPRQRKQRTGLRQFLREVRQELK
                     RVAWPSRKELVSYSAVVLVAVTLVTVYVFGLDQAFGQLVFWIFR"
     gene            3224..3754
                     /gene="nusG"
                     /locus_tag="VNU01_09630"
     CDS             3224..3754
                     /gene="nusG"
                     /locus_tag="VNU01_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWH32917.1"
                     /translation="MPGDWYVVHTYAGYEAKVKANLENRIQSMNMEDRIYEVVIPTED
                     AVEIKAGKKQTVQRKVFPGYVLVRMDLDDDSWYVVRNTPAVTGFVGPPGARPVPLSLK
                     EVEAILAEPEEGEEVVTSVEYEEGENVRVTSGPFADFTGTISEINADQSKLKVLVSIF
                     GRETPVELGFDQVAKL"
     gene            3795..4217
                     /gene="rplK"
                     /locus_tag="VNU01_09635"
     CDS             3795..4217
                     /gene="rplK"
                     /locus_tag="VNU01_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWH32918.1"
                     /translation="MAKKVMALIKLQIPAGQATPAPPVGPALGQHGVNIMEFCKAYNE
                     RTAAQQGNIVPVEITVFEDRSFTFVTKTPPAARLLLKAANLEKGSGVPNRTKVGTISR
                     DKVREIAEQKMADLNAIDVEGATKIIEGTARSMGLVVE"
     gene            4286..5020
                     /gene="rplA"
                     /locus_tag="VNU01_09640"
     CDS             4286..5020
                     /gene="rplA"
                     /locus_tag="VNU01_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWH32919.1"
                     /translation="MAQRGKKYRAALEKIEEGRVYSPVAATKLVKETVTTKFDATVEL
                     AVRLGIDPRKADQMVRGSVPLPHGTGSTARVAVFAEGPKAQEGRDAGADVVGVEEITA
                     MIEAGNLEFDAIIATPDQMGKIGRYGKVLGPRGLMPNPKTGTVTMDVAKAVEEIKAGK
                     IEYRTDRQANVHVVLGKASFTAEQILDNYNAVMDELLRQRPAAAKGRYLRSVHLSTVM
                     GPSIPVDHAKKESEDAEQAGVVAQTA"
     gene            5051..6103
                     /locus_tag="VNU01_09645"
     CDS             5051..6103
                     /locus_tag="VNU01_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH32920.1"
                     /translation="MTAEHLRRLPFDHFARYQLAAEVVDATRRVGASRVLDIGGGPGS
                     LAAFCPDDRVISSDLHLPSHWHAAAPDLVLADGAKLPFADSTFDIVVTLDTLEHVPPE
                     RRPDLLSEALRVSRGYVLVVCPCGTPGVPEADAALLSFVRQRFGEEFETVGVLQEHLG
                     YGHPDPQQVLRDMRRAGADVESFPSGRLDRWLPMMLLFYNLMALGKDDPVERVQAWYN
                     ALFYRDDLRAPSYRQAFLARVAGADGPAPEEIVGRLLPQGPPLAADTSALEALRIGLT
                     EELVGTVDHYRAQLADLERRLAEATVESDRQRTRAELAEAHAAGLEAFRQQVLNHPLV
                     KATRPLRRTVRALGRR"
     gene            complement(6110..7135)
                     /locus_tag="VNU01_09650"
     CDS             complement(6110..7135)
                     /locus_tag="VNU01_09650"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HWH32921.1"
                     /translation="MSSAPHVRDVKGVIRDLLDRHGRIRAREVLEALGHGISRQAVHA
                     HLRAMIDAGEVQKSGVGKATWYEPAVAFARTYATEDLDESAVWSDVRAAVPLLRDLGE
                     PADGTMAYVFTKMVNNAIDHSGSPTVTVRARLDAPSIVVDVEDAGVGLFAKVASALES
                     ATLLEAAQRLMVGKFTTWPERHTGEGIFFSSRSVDVFVAEANGLRWTVDNEREDWAIG
                     EVDPRTGTLVRLALDPARARPLAEVFSRFTDLEDHRFDRTRVVVKMLDAGVRFVSRSE
                     AKRLVAELEPFDHVVLDFAGVTEVGQGFVDELFRVWAGAHPQKELQPANMSDAVRFMV
                     QRGRPRT"
     gene            7466..8155
                     /gene="rplJ"
                     /locus_tag="VNU01_09655"
     CDS             7466..8155
                     /gene="rplJ"
                     /locus_tag="VNU01_09655"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWH32922.1"
                     /translation="MPRPEKVAVVDEVREKLTSSTATVLTEYRGLTVSQLAALRAELR
                     KNNAEYKVAKNTLTRIAAREAGFEVPDELLSGPTALAFCADDPVGVAKALRTFAKANP
                     SLVVKGAVLDGRLIDAAETSRLADLETREELLAKMAGMIQTLIAQPARLAQASLTKMA
                     QLLAAYSEKKAAEPQPAAEAAAPAEAAEESAPEEAAAPEAEEAKAPAETEPAAEAPAE
                     EPAAEEAATEE"
     gene            8376..8756
                     /gene="rplL"
                     /locus_tag="VNU01_09660"
     CDS             8376..8756
                     /gene="rplL"
                     /locus_tag="VNU01_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWH32923.1"
                     /translation="MSTDDLLESFKEMTLLELSEFVKKFEEEFDVTAAAPVAVAAAPA
                     AGGGAGAEAAEEKDAFDVILTDAGDKKIQVIKEVRALTNLGLKEAKDLVDGAPKPVLE
                     GANKETAEKAKEALEGAGAKVELK"
     gene            9154..12738
                     /gene="rpoB"
                     /locus_tag="VNU01_09665"
     CDS             9154..12738
                     /gene="rpoB"
                     /locus_tag="VNU01_09665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWH32924.1"
                     /translation="MATSRLRTRANDDRRLSFAKIHEPLPVEELDLVAIQRHSFEWLR
                     EAGLREVFEEISPIEDFTGQMALSFSEHRFEEPKHSVEECKEKDMTYSAPLFVTAEFV
                     NRTTGEIKSQTVFMGDFPMMTGGGTFVINGTERVVVSQLVRSPGVYFDSSIDKATGRD
                     VFGCKIIPSRGAWLEFEIDKRGFAGVRVDRKRRQHISVLYRALKGIVFNEATGEYELA
                     DRETLESPVPAEEILAFFGESEVIARTLEKDTLLTPSDALQDIYRKLRPGEPPTAESA
                     GGLLINLFFNNKRYDLAKVGRYKVNKKMGEELVRLGLRTPDEAKPLAGGEVPAPLTEA
                     AKNKVKAAAAAYLPGEAASPSFAMILTKEDILATMSYLIRLGQEQPGYDVDDIDHFGN
                     RRLRSVGELIQNQVRIGLSRMERVVRERMTTQDVEAITPQTLINIRPVVASIKEFFGA
                     SQLSQFMGQTNPLEGLTHKRRLSALGPGGLSRERAGFEVRDVHVSHYGRMCPIETPEG
                     PNIGLIGSLATYARINEFGFIETPYRKVRNGVVTEKIEYLTADEEDRQVIAQANAPLD
                     DKGNFVNPFVLCRAKNGEVREVPPVEVDYMDVSPRQIVSVAAAMIPFLEHDDANRALM
                     GTNMQRQAVPLLRSQAPLVGTGLESKAARDAGDVVVADSDGVVVEVAADRIIVKTSDG
                     KLEKHFLRKFERTNQGTCYNQRPIVDEGDEVKAGQVIADGPCNDLGEMALGANLLVAF
                     MSWEGFNYEDAIILSERLVKEDVLTSIHIEEHEVDARDTKLGAEEITRDIPNVSDEVL
                     ANLDERGIIRIGAEVNPGDILVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKV
                     PHGEVGRVIGVRTFSREDGDEMPPGVNELVRVYVAQKRKISDGDKLAGRHGNKGVIAK
                     ILPVEDMPFLADGTPVDIVLNPLGVPSRMNVGQVLETHLGWVGAQGWEFDKKPQWFER
                     MGWGDDKLSRDPGVLATPVFDGCKEDELTSLLENTKPNRDGVRLVDGTGKALVFDGRS
                     GEPIDQPITVGYMYILKLLHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEV
                     WALEAYGAAYALQEILTVKSDDVLGRVKVYEAIVKGENIPEPGIPESFKVLVKEMQSL
                     CLNVEVLSATGQEIEFRESDDDAFRAAEELGIDLSRPERYGDEFSIGS"
     gene            12784..>13512
                     /locus_tag="VNU01_09670"
     CDS             12784..>13512
                     /locus_tag="VNU01_09670"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWH32925.1"
                     /translation="MLDVNNFDELRIGLATQDQIRMWSNGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTRDWECYCGKYKRVRFKGIICERCGVEVTRSKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYIITSVDAEARHSDLPKLEEEIHAEKR
                     KIEKQLEIDRDQLLKELEAELADLEESGAKAEQARKKRREIEKQIKTVTGNAQARSQR
                     LDEVLDTFRGLAPKQLIADELLYRE"
BASE COUNT         2309 a         4650 c         4751 g         1802 t
ORIGIN      
        1 gagatggtga tgccgggcga caacaccgag atggtggtgg agctgatcca gccgatcgcg
       61 atggaggacg gtctgcgctt cgccatccgt gagggtggcc gcaccgtcgg cgccggccgc
      121 gtcaccaaga tcctgaagta gttcggagtg cctcgcctcg cgctcggcgg aaaagccctt
      181 tagcgctcgc gccgaggcga ggcactcctc aagcattggc gtggcgctcc ggcgcggggc
      241 cccacgacaa gccagtcgca ccaccggaag cgccagggcc ggcaagaccg acagccgctt
      301 cctctcgctg cgtcgagggg agcgccccgg gcctgaggcc tcggtccctg acccggccgg
      361 cggtcctttg tgcgcctttc gggcgtgcga gtactctcga tgaactggcc ggaccgcgac
      421 gtgagcacag gaagtaggta gccgagatgc cgaagagtga tgttcggccg aagatcacgc
      481 tggcttgcac cgagtgcaag gagcgcaact acatcaccac caagaaccgc aacacgcagc
      541 gggaacgggt ggagctgaag aagtactgcc cgcggtgcaa gacgcaccag ccgcaccgcg
      601 agaccaagta gcgcatccgc cgcgacgcga gccccgccgt aggatcccgg cggggctttc
      661 gcgtcccccg caagttgggt gcgttccggg cgctccctgg cccagactgc acccgcacga
      721 gccggaaacc tggggaaagg tgttggcgtg gggcggttgc agggccgggt ggcggtcatc
      781 accggcgcgg cacgcggcat cggcgaggcc accgccaggc tcttcgccgc cgagggcgcg
      841 acgctcgcgc tgaacgacgt cgacgaggag cccctgcggg aactggcggc gtccctggac
      901 gccgtggccg tgccgggcga cgtggcgcgc gacgccgagc gcatcgtggc cgaggcgctg
      961 gccgcccatg gcgcgatcga catcgtggtg aacaacgcgg gcacgcgcgc cgacgcgccg
     1021 ttccacctca tgaccgccga gcagtgggac gcggtgttcg acgcgtgcct gaccggcacg
     1081 ctgcgcatgt gccaggccgt cgcgccgcac ttccgcgagc gcgccatggc cgagctcgcg
     1141 gagcacggcg ccgtcgccca ccaccgcaag atcaccaaca ccgcgccggc cagcttcctc
     1201 gtcggcagcc ccggccaggc caacctcacc gcggcgggcg gcgcggtcgt cggcctcacc
     1261 aggacgctcg cgcgagagct gggcgggttc gcggtgaacg tcaacgcggt cgctcccggc
     1321 ttcgtcgaga cgcgcctgac ggcggccgag caccccgacg ggggcgcggg catggccgag
     1381 ccgctgcgcc agatgaccaa ggccatgacg gccctgggcc ggcacgggca gccgggcgat
     1441 ctcgcgcgcg tgcacctgtt tttggcatca tccgacgccg acttcgtgac cggtgtcacg
     1501 atccccgtcg cagggggcct gctggggacg tgcgtgtgaa gggagacgcg tcatcgcgct
     1561 gaacaaagcc cgtgtcgggc accgctaccc cagctaccgc tacgaggtca gccgggagaa
     1621 gatccgcgag tacgccaacg ccacgggcgt cagcgacccg cagtacgccg ccgacgaggg
     1681 cgacgtcgtc gcgccgccga tgttcgcggc ctgcttcacc gtcatccgcg gcggcgccgg
     1741 gatgttcggc gacgcggagc tgggcgccca cttcgcgctg gtccacggct cgcaggagta
     1801 cgagttccac cgcccggtgc gcctgggcga cgtgctggag tgcacgccgt tcatcgcgga
     1861 catccagtgg aagggacgca acgagttcct cacgctgcag gtggactgca acgacgcgac
     1921 cacgggcgag cccgtggtga ccagccgcgg cgtgatcgtg ttcctcggct cggctcccca
     1981 gaatcccgag cagcaggagg cgtcctagat gcccaagttc gacgaggtca aggtcgggga
     2041 ccagatcccc ggcgagtcca agaccatcga ccagatgcag ctgatccagt acgccggcgc
     2101 cagcggcgac ttcaacccgc tgcactggca ggaggagttc gccggcaagg tcagcccgac
     2161 cggcgggatc atcgctcacg gcatgtacaa cgccgggctg atgtcgatgg tcgtcacccg
     2221 ctgggctggc gggccggaga acgtcaagcg gatcaagacc agcttccggg cgccctgccc
     2281 gctgggcgcc acggtgagct tcggcgggga ggtgaccgcc ctggacgagg cggcgcgcac
     2341 cgcgaccttg gccatctggg ccgagctcga ggacggctcg aaggtgatcg atcgcggctc
     2401 gagcgaggcc gtcgtccagc tcgactgacc gtgccggtcc gggccccggt tgccggggcc
     2461 cggatggcgc gcctatactg gcctcgacgc tgcccccggg cagtgttttt gttgtcccag
     2521 gcaacaccac ctggggaccc aggggcgtag ctcaactggc agagcgtcgg tctccaaaac
     2581 cgaaggccgg gggttcgagt ccctccgccc ctgcgacggc ggccgaccct ggccgcaggt
     2641 atggcgatga ggcgatcatg agtcgggaga cacagcgcga gatggatgag gagcgtcgcg
     2701 acgagcggcc ggccgcgccg tccccgcgcc agcgcaagca acgcaccgga ctgcggcagt
     2761 tcctgcgcga ggtgcgccag gagctcaagc gcgtggcatg gccgtcgcgc aaggagctgg
     2821 tgtcctactc ggcggtggtc ctcgtggccg tgacgctggt gaccgtgtac gtgttcggcc
     2881 tcgaccaggc cttcgggcag ctggtgttct ggatcttccg atgagcgacg cttcccccta
     2941 cgacgacgag atgccggccg agagcgactc cccggaggct gacgcgctgc tgtccggccc
     3001 caccggggcg cccgaggagg tcgagcaggc cgaggcaggc gatctggacg agctgctgtc
     3061 ggccgcgggc gccggcgacg gcgcggccgc caaggaggac ctggacgcgc tgatcgccgc
     3121 ggacaccgag cagcgtccgg ccaataccgc cgtggacacc gccgagccgg agacggacaa
     3181 cggcgcgccg gccaagcgca accgcggcct ggacttcatg cggctgccgg gcgactggta
     3241 cgtcgtgcac acctacgccg gctacgaggc gaaggtgaag gccaacctcg agaaccgcat
     3301 ccagtccatg aacatggagg accggatcta cgaggtggtc atcccgacgg aggacgccgt
     3361 cgagatcaag gccggcaaga agcagaccgt gcagcgcaag gtcttccccg gctacgtgct
     3421 ggtgcgcatg gacctggacg acgactcctg gtacgtggtg cgcaacacgc ccgcggtcac
     3481 cggtttcgtc gggcctccgg gtgcgcggcc cgtgccgctg tcgctgaagg aggtcgaggc
     3541 gatcctggcc gagcccgagg agggcgagga ggtcgtcacg tcggtcgagt acgaggaggg
     3601 cgagaacgtg cgcgtcacgt ccggcccgtt cgcggacttc accggcacca tcagcgagat
     3661 caacgccgac cagtcaaagc tcaaggtgct cgtgtccatc ttcggccgcg agacccccgt
     3721 ggagctcggt ttcgaccagg tcgccaagct ctagcggcga ccgccaacca cacacaggaa
     3781 gcaggtacca agagatggcc aagaaggtca tggccctgat caagctccag atcccggccg
     3841 ggcaggcgac ccccgccccg ccggtcggtc ccgcgctggg tcagcacggg gtcaacatca
     3901 tggagttctg caaggcctac aacgagcgca ccgcggcgca gcagggcaac atcgtcccgg
     3961 tcgagatcac cgtgttcgag gaccgctcgt tcacgttcgt caccaagacg ccgccggccg
     4021 cgcgcctgct gctgaaggcg gcgaacctcg agaagggctc cggcgtgccc aaccgcacca
     4081 aggtcggcac catctcgcgg gacaaggtgc gtgagatcgc cgagcagaag atggcggacc
     4141 tcaacgcgat cgacgtggag ggcgccacca agatcatcga gggcacggcc cgcagcatgg
     4201 gcctcgtcgt cgagtagtcc gatcaccatc gacaacgtgg gaggacggct tcgggccgtc
     4261 cgtctgcacc actggaggca ctgacatggc acagcgtggc aagaagtacc gggcggctct
     4321 ggagaagatc gaggagggcc gggtgtactc gccggtcgcc gcgacgaagc tcgtgaagga
     4381 gaccgtcacc accaagttcg acgcgaccgt cgagctcgcc gtgcggctgg gcatcgaccc
     4441 ccgcaaggcc gaccagatgg tgcgcggctc cgtgccgctg ccgcacggca ccggcagcac
     4501 cgcgcgcgtc gcggtgttcg ccgagggccc caaggcccag gagggccgtg acgccggcgc
     4561 ggacgtggtg ggtgtcgagg agatcaccgc gatgatcgag gcgggcaacc tcgagttcga
     4621 cgcgatcatc gccacgcccg accagatggg caagatcggc cgctacggca aggtcctggg
     4681 cccgcgcggc ctcatgccga accccaagac cggcacggtg accatggacg tcgccaaggc
     4741 cgtcgaggag atcaaggccg gcaagatcga gtaccgcacc gaccgccagg ccaacgtcca
     4801 cgtcgtgctc ggcaaggcgt cgttcaccgc cgagcagatc ctggacaact acaacgcggt
     4861 gatggacgag ctgctgcgcc agcgcccggc ggccgcgaag ggccgctacc tgcgctcggt
     4921 gcacctgtcc acggtgatgg ggccgagcat cccggtcgac cacgccaaga aggagtccga
     4981 ggacgccgag caggcgggcg tcgtcgccca gaccgcctag ccggtcgcgg gccgggccga
     5041 ggagggcagg atgaccgcgg agcacctgcg gcgcttgccc ttcgaccatt tcgcgcgcta
     5101 ccagctcgcc gcggaggtgg tcgacgccac acggcgcgtg ggcgcctcgc gcgtgctgga
     5161 catcggcggg ggaccgggct ccctggcagc gttctgcccg gacgaccgcg tcatctccag
     5221 cgacctgcac ctgcccagcc actggcacgc cgccgcgccc gacctcgtgc tcgccgacgg
     5281 cgcgaagctg ccgttcgcgg actccacctt cgacatcgtg gtgaccctgg acacgctgga
     5341 gcatgtgccg ccggagcggc gcccggacct gctgtcggag gcgctgcgcg tctcccgcgg
     5401 ttacgtcctg gtggtgtgcc cgtgcggcac gccgggcgtg ccggaggccg acgcggcgct
     5461 gctgtcgttc gtgcgccagc gtttcggcga ggagttcgag accgtcggcg tgctccagga
     5521 gcacctgggc tacgggcacc ccgacccgca gcaggtgctg cgcgacatgc gccgcgcggg
     5581 cgcggacgtg gagtcgttcc cgagcgggcg cctggaccgc tggctgccca tgatgctgct
     5641 cttctacaac ctcatggcgc tgggcaagga cgacccggtc gagcgcgtcc aggcctggta
     5701 caacgcgctg ttctaccgcg acgacctccg cgcgccgagc taccgccagg cgttcctggc
     5761 cagggtcgcg ggcgccgacg gtccggcgcc ggaggagatc gtcggccggc tgctgccgca
     5821 aggcccgccg ctggcggccg acaccagcgc gctcgaggcg ctgcgcatcg gcctcaccga
     5881 ggagctcgtg ggaaccgtcg accactaccg cgcccagctt gcggacctgg agcggcgcct
     5941 ggcggaggcc accgtcgagt cggaccggca gcgcacccgg gcggagctcg ccgaggcgca
     6001 cgccgccggc ctggaggcgt tccgccagca ggtgctgaac cacccgctgg tgaaggcgac
     6061 ccgcccgctg cggcgcacgg ttcgagccct gggccggcgc tgacgcggct caggtccggg
     6121 ggcgtccccg ctgcaccatg aaccggacgg cgtcgctcat gttggccggc tgcagctctt
     6181 tctgcgggtg ggccccggcc cagacccgga acagctcgtc gacgaagccc tggccgacct
     6241 ccgtgacacc ggcgaagtcg agcaccacat ggtcgaacgg ctccagttcc gccaccagcc
     6301 gcttggcctc ggaacgcgag acgaagcgga ccccggcgtc gagcatcttc accaccaccc
     6361 gcgtccggtc gaatcggtgg tcctccagat cggtgaagcg cgagaagacc tcggcgaggg
     6421 gtctggcacg cgccgggtcc agcgccagcc tgaccagcgt gcccgtccgt gggtccactt
     6481 cgccgatggc ccagtcctct cgctcgttgt ccacggtcca ccgcagcccg ttcgcctccg
     6541 ccacgaacac gtccacgctg cgcgacgaga agaagatccc ttccccggtg tgacgctccg
     6601 gccatgtggt gaacttgccc accatcagcc gctgtgccgc ctccagcaag gtggccgact
     6661 ccagggcaga ggccaccttg gcgaacagcc ccacgccggc gtcctccacg tccaccacga
     6721 tggacggcgc gtccaggcgc gcgcggacgg tcacggtggg cgagcccgag tggtcgatgg
     6781 cgttgttcac catctttgtg aacacgtagg ccatggtgcc gtccgccggt tcgccgaggt
     6841 cgcgcagcag gggcacggca gcgcggacat cgctccagac ggccgactcg tcgaggtctt
     6901 cggtggcgta cgtgcgcgcg aaggcgaccg cgggctcgta ccaggtcgct ttcccgacgc
     6961 cgcttttctg cacttcgccg gcgtcgatca tggcgcgcaa gtgggcgtgc accgcttgcc
     7021 gtgagatccc gtggccaagc gcctccaaga cctcgcgcgc ccggatgcgg ccatgccggt
     7081 ccagcaggtc gcggatgacg cccttgacgt ccctgacatg cggagcgctt gacatcttgt
     7141 cgcaatcacc ttgttgagca gcgtgttagt tgacatcttg gcggtagcgc gcccaacgta
     7201 cgagtttgtg tgaacttgtc aagaggcgtc cgttgcgacg cgcacgtcct caggtttgca
     7261 agcggtgggg gacggcggct acagtggccg ttcaccgcag accgctcgga tcgcgccgct
     7321 gtgcgcgcga agaagggccg gggcagccgg ccgccagcgc aggtgaggac gaagggcacc
     7381 gtcgccctgc cgtgctcatg tgcgcctccc ggtccgcggg aggctttttc ttttgctaca
     7441 gccacgcagg aaccaaggag ccacgatgcc ccgcccggag aaggtcgcgg tcgtcgacga
     7501 ggtgcgcgag aagctcacgt cgtccaccgc cacggtgctc accgagtacc gcggcctgac
     7561 cgtgtcccag ctcgccgcgc tgcgcgccga gctgcgcaag aacaacgccg agtacaaggt
     7621 cgcgaagaac acgctgaccc gtatcgcggc gcgcgaggcc gggttcgagg tgccggacga
     7681 gctgctgtcc ggtcccaccg cgctggcgtt ctgcgccgac gacccggtcg gcgtcgccaa
     7741 ggcgctgcgc acgttcgcga aggccaaccc gagcctcgtc gtgaagggcg ccgtgctcga
     7801 cggccgcctg atcgacgcgg cggagaccag ccgcctggcc gacctggaga cccgcgagga
     7861 gctgctggcc aagatggccg gcatgatcca gacgctgatc gcccagccgg cacgcctggc
     7921 gcaggccagc ctgaccaaga tggcgcagct gctggccgcc tactccgaga agaaggcggc
     7981 cgagccccag cccgccgccg aggccgcagc tccggcagaa gccgccgagg agagcgcccc
     8041 ggaagaggcc gccgctccgg aggccgaaga agccaaggcc ccagcagaga cggagcctgc
     8101 ggcggaagct cccgcggagg agccggccgc cgaggaggca gccaccgagg agtagacgcc
     8161 gaggcgcgcg cccgacgagg cgaactcagg gtgccggccg caggccggcg ggtcccctgg
     8221 agagcctcgg agggatgcgc gacacggcgg cgacaccgcg ggaggaaccg cggcggcgac
     8281 ccacgccagg cacggcgcaa cgccgggccc ggcgagcacc accagacgtc agcagacgaa
     8341 cgcaccacac cgaggagagc acgaccatgg cgaagatgag caccgacgac ctgctggagt
     8401 ccttcaagga gatgaccctc ctggagctct ccgagttcgt gaagaagttc gaggaggagt
     8461 tcgacgtcac cgcggccgcg ccggtcgccg tggccgccgc tcccgccgcc ggcggtggcg
     8521 ccggcgccga ggccgcggag gagaaggacg cgttcgacgt gatcctgacc gacgcgggcg
     8581 acaagaagat ccaggtcatc aaggaggtgc gcgcgctgac gaacctgggc ctgaaggagg
     8641 ccaaggacct cgtcgacggt gcccccaagc cggtcctgga gggcgccaac aaggagacgg
     8701 ccgagaaggc gaaggaggcc ctggagggcg ccggcgccaa ggtcgaactc aagtaggcac
     8761 ggctcctcgg tgttctcgcg tccggggcgg ttcgccgccc cggacggcac ccgggcccgg
     8821 gagcacgccg tccgccggtg ggctcgtccc acgggccgcg ttgacgaatc gacgcgtcgg
     8881 tggcaggctg gccccccaag ccacccgcca tccgcacgag gagttgcgtg ccggcgacca
     8941 cccgcctccg aagccatcgg ggccgagaag agccgttgag agcagtgtag ccgtcgctac
     9001 actgttcttt cgcgttttca caaggcccga atcggaccat tcgggctgca caaggcgcca
     9061 tcaggcgcgc tgaacgggct gaccagctcg cgtcccgtcg gcagcgcccg cgtcacctga
     9121 gcgaattgca tcgctactgg aggagtagga accttggcga cctcgcgtct ccgcacccga
     9181 gccaacgacg accggcgcct gtcgttcgcg aagatccacg aacctcttcc cgtggaggag
     9241 ctggatctcg tcgcgatcca gcgccactcc ttcgagtggc tgagggaagc cggtctgcgg
     9301 gaggtcttcg aggagatctc gcccatcgag gacttcaccg gccagatggc gctgtccttc
     9361 tccgagcacc gcttcgagga gcccaagcac tccgtggagg agtgcaagga aaaggacatg
     9421 acctactcgg cgccgctgtt cgtcacggcc gagttcgtca accgcaccac gggcgagatc
     9481 aagtcccaga cggtcttcat gggcgacttc ccgatgatga ccggtggcgg caccttcgtg
     9541 atcaacggca ccgagcgtgt cgtggtgtcc cagctggtgc gttccccggg cgtgtacttc
     9601 gactcctcca tcgacaaggc cacgggccgt gacgtcttcg gctgcaagat catccccagc
     9661 cggggcgcgt ggctggagtt cgagatcgac aagcgcggat tcgcgggcgt gcgcgtggac
     9721 cgcaagcgcc gccagcacat cagcgtgctg taccgcgccc tgaagggcat cgtcttcaac
     9781 gaggcgaccg gcgagtacga gctggccgac cgcgagacgc tcgagtcgcc cgtgccggcc
     9841 gaggagatcc tggcgttctt cggcgagtcc gaggtcatcg cgcgcacgct ggagaaggac
     9901 acgctgctca cgccgagcga cgcgctgcag gacatctacc gcaagctgcg cccgggcgag
     9961 ccgccgacgg cggagtccgc gggcggcctg ctgatcaacc tgttcttcaa caacaagcgc
    10021 tacgacctgg ccaaggtcgg ccgctacaag gtcaacaaga agatgggcga ggagctggtc
    10081 cgcctgggcc tgcgcacgcc cgacgaggcc aagccgctcg cgggcggcga ggtgccggcg
    10141 ccgctgaccg aggcggcgaa gaacaaggtg aaggccgcgg cggcggcgta cctgcccggc
    10201 gaggcggcct cgccgtcgtt cgcgatgatc ctcaccaagg aggacatcct ggcgacgatg
    10261 tcctacctga tccggctggg ccaagagcag cccggctacg acgtcgacga catcgaccac
    10321 ttcggcaacc gccgcctgcg cagcgtcggc gagctgatcc agaaccaggt gcggatcggc
    10381 ctgtcgcgca tggagcgcgt ggtgcgcgag cgcatgacca cgcaggacgt cgaggcgatc
    10441 accccgcaga cgctgatcaa catccgcccc gtggtggcgt ccatcaagga gttcttcggg
    10501 gccagccagc tgtcgcagtt catgggccag accaacccgc tggagggcct cacgcacaag
    10561 cggcgcctgt cggccctcgg ccccggcggc ctgtcgcgtg agcgcgccgg cttcgaggtc
    10621 cgtgacgtgc acgtgagcca ctacggccgc atgtgcccga tcgagacgcc ggaaggcccg
    10681 aacatcggcc tgatcggctc gctggcgacc tacgcgcgca tcaacgagtt cggcttcatc
    10741 gagacgccgt accggaaggt ccgcaacggc gtcgtcacgg agaagatcga gtacctgacg
    10801 gccgacgagg aggaccggca ggtcatcgcg caggcgaacg cgcccctgga cgacaagggc
    10861 aacttcgtca acccgttcgt gctgtgccgc gcgaagaacg gcgaggtccg cgaggtgccg
    10921 cccgtcgagg tggactacat ggacgtctcg ccgcgccaga tcgtgtcggt cgccgcggcg
    10981 atgatcccgt tcctggagca cgacgacgcc aaccgcgccc tcatgggcac gaacatgcag
    11041 cgccaggccg tgccgctgct gcgcagccag gcgccgctgg tgggcaccgg gctggagtcc
    11101 aaggccgctc gcgacgccgg cgacgtcgtg gtggccgaca gcgacggcgt ggtcgtggag
    11161 gtcgccgccg accgcatcat cgtgaagacg tcggacggca agctcgagaa gcacttcctc
    11221 cgcaagttcg agcgcaccaa ccagggcacg tgctacaacc agcgcccgat cgtcgacgag
    11281 ggcgacgagg tcaaggccgg ccaggtgatc gccgacgggc cgtgcaacga cctcggcgag
    11341 atggcgctgg gcgccaacct gctggtcgcg ttcatgtcct gggagggctt caactacgag
    11401 gacgcgatca tcctctcgga gcgcctggtc aaggaggacg tgctcacctc gatccacatc
    11461 gaggagcacg aggtcgacgc ccgcgacacc aagctgggcg ccgaggagat cacccgggac
    11521 atccccaacg tctccgacga ggtgctggcc aacctggacg agcgcgggat catccgcatc
    11581 ggcgcagagg tcaaccccgg cgacatcctg gtcggcaagg tcaccccgaa gggcgagacg
    11641 gagctgaccc cggaggagcg cctgctgcgc gcgatcttcg gcgagaaggc gcgcgaggtg
    11701 cgcgacacgt cgctgaaggt cccccacggc gaggtcggcc gcgtcatcgg cgtgcgcacg
    11761 ttcagccgcg aggacggcga cgagatgccg ccgggcgtca acgagctggt ccgcgtgtac
    11821 gtggcgcaga agcgcaagat ctccgacggc gacaagctgg ccggccgcca cggcaacaag
    11881 ggcgtgatcg ccaagatcct gcccgtggag gacatgccgt tcctggcgga cggcacgccc
    11941 gtcgacatcg tgctgaaccc gctgggcgtg cccagccgca tgaacgtggg ccaggtgctg
    12001 gagacgcacc tcggctgggt cggcgcgcag ggctgggagt tcgacaagaa gccgcagtgg
    12061 ttcgagcgca tgggctgggg cgacgacaag ctgtcgcgcg atccgggcgt gctggccacg
    12121 ccggtgttcg acggctgcaa ggaggacgag ctcaccagcc tgctggagaa caccaagccg
    12181 aaccgtgacg gcgtccgcct ggtcgacggc acgggcaagg cgctggtgtt cgacggccgc
    12241 tccggcgaac cgatcgacca gcccatcacc gtgggctaca tgtacatcct gaagctgctg
    12301 cacctggtgg acgacaagat ccacgcccgc tccaccggcc cgtactcgat gatcacccag
    12361 cagccgctgg gcgggaaggc gcagttcggc ggccagcgct tcggcgagat ggaggtctgg
    12421 gccctggagg cgtacggcgc cgcctacgcc ctgcaggaga tcctcaccgt caagtccgac
    12481 gacgtgctgg gccgcgtgaa ggtctacgag gccatcgtca agggcgagaa catcccggag
    12541 cccggcatcc ccgagtcctt caaggtgctc gtcaaggaga tgcagtcgct gtgcctgaac
    12601 gtcgaggtgc tctcggcgac cgggcaggag atcgagttcc gcgagtcaga cgacgacgcg
    12661 ttccgcgccg ccgaggagct cgggatcgac ctgtcccgcc cggagcgcta cggcgacgaa
    12721 ttctcgatcg gcagctagcc acacccacga agcgcttccg ccacgcggaa agaggagcga
    12781 gcagtgctcg acgtcaacaa ctttgatgag ctgcgcatcg gcctggccac gcaggaccag
    12841 atccgcatgt ggtccaacgg cgaggtcaag aagccggaga ccatcaacta ccggacgctg
    12901 aagccggaga aggatggcct gttctgcgag aagatcttcg gtcccacgcg ggactgggag
    12961 tgctactgcg gcaagtacaa gcgcgtgcgc ttcaagggga tcatctgcga gcgctgcggc
    13021 gtcgaggtca cgcgctccaa ggtgcgccgt gagcgcatgg gccacatcga gctcgccgcg
    13081 ccggtgacgc acatctggta cttcaagggt gtgccgtcgc gcctgggcta cctgctcgac
    13141 ctggcgccca aggacctcga gaaggtcatc tacttcgcgg cctacatcat cacctccgtc
    13201 gacgccgagg cgcgccacag cgacctgccg aagctggagg aggagatcca cgccgagaag
    13261 cgcaagatcg agaagcagct tgagatcgac cgcgaccagc tcctgaagga gctggaggcg
    13321 gagctggccg acctggagga gtccggggcc aaggccgagc aggcgcgcaa gaagcgccgc
    13381 gagatcgaga agcagatcaa gaccgtgacc ggcaacgcgc aggcgcgctc ccagcgcctg
    13441 gacgaggtgc tggacacgtt ccgcggcctc gcccccaagc agctgatcgc cgacgagctg
    13501 ctgtaccgcg ag
//
LOCUS       DATMKC010000326         4119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_341462, whole genome shotgun sequence.
ACCESSION   DATMKC010000326 DATMKC010000000
VERSION     DATMKC010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341462"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VNU01_09675"
     CDS             complement(<1..276)
                     /locus_tag="VNU01_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH32926.1"
                     /translation="MRIDKDLVAASATPLVLAILAEGESYGYAILKRINQLSGGEMDW
                     TDGMLYPLLLRQCRPPGDGDRVVPAAPPLRRDAPLGGHVPEALEAVQQ"
     gene            511..1029
                     /locus_tag="VNU01_09680"
     CDS             511..1029
                     /locus_tag="VNU01_09680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWH32927.1"
                     /translation="MSDAPGFSSQMAIERFLRASQGEPFQDGELLPFHLPECYGCGPE
                     NESGLHLSVRAAPENAVAGEYTFTRRLGGAPGVAHGGAVAALLDDLFGFVLVRILVPA
                     VTRDLQVRYRRPVHLHTPCALSARLVGREGREVHMAGELRQEGQVKAAAEAVFVELEP
                     ERLLNRYERLEP"
     gene            1029..1628
                     /locus_tag="VNU01_09685"
     CDS             1029..1628
                     /locus_tag="VNU01_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH32928.1"
                     /translation="MSRQVPPDIAGRLPAALHLFLDQGLDGTRVDAIVAATGIPKATL
                     YYHFDSKEAIFAFLLRELLDELAEAVDAAKRGRGTARERLVAVIEAIVGAMAANPTSC
                     LVLLSNLSRAGRMPEIAGSVQAAFHAPVEELLVEGSRDGSLRATGPATASAVFGAVVL
                     VSLHHLIMTGDIPVRATVDSVGSLLLHGLATGAAEGTDG"
     gene            1621..>4119
                     /locus_tag="VNU01_09690"
     CDS             1621..>4119
                     /locus_tag="VNU01_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWH32929.1"
                     /translation="MAERLLFTALRHPKAVVGAWLLAAIVLLVTAPSLRDVGTLDTAE
                     LLPSTSPSVVAAEASRRAFPEDLGRNSAVVIVRRPGGLLDTDRAWLAQTAERLAAHPL
                     AAATRVLSAAANPAMGTALRSPDGAAELLLVELEGSPFEPETAEAIEQLRQVVRADAP
                     AGVDAAVTGIAALGADQGEAITASIDQTAVITILLILLILWWVYRAPLAVLLPLATIA
                     VAFVVSRSLVAFLAASGFTVAGHAETFMVVLVLGAGTDYTLFLVSRYAEELAQGQPLP
                     DALRRTVRVIAGVIAAAAATVIVGFLSMLTADFGMYRSFGPAIALAIAVTLLVGLTFT
                     PALLALTGKAAFWPSGPVKASRAHAASGRWERVATLVRHRPIEVLLAGAILLLVPSAG
                     LAWLTPSMDLVGDLPPAAQSRQGFEILDQHFPAGALAPVQVVVAHDRPLTDDTAFAAL
                     DDLTDRLNEQPGIALARSITQPLGAPLTPDTIGRLTGGGFDPRALGLDPDVVDLTPLL
                     QAIEQPGGLRLTWSLLERYPQFNDLLAPFLSDDRQTTRLIVELDGNPFVPESLGKLDA
                     LPHVVADTLSGTSLAGAQVHLAGSPAYYRDVQDVAARDLALITVVLMLGIFLVLAALL
                     RSLVAPIYLLATVLLSYAATLGLTTWMSMLVLGTADMAFWIPPFLLVILVALGADYNI
                     FIMGRIREEVDAGSDIGAATARGLVLTGGVITSAGLILAGTFAVLMLAPMPNLRLIGF
                     AVTVGVLLDTFVVRSVLVPAATVLLDRWALWPSAHPRVRALRLAPATIAGLVVLLLAA
                     AALPSLPAEPVVVAHAAVEQQGAPDRQPPADDPSD"
BASE COUNT          538 a         1512 c         1391 g          678 t
ORIGIN      
        1 ctgctgcacc gcctccagcg cctcgggtac gtgaccaccg agtggcgcgt cccgccggag
       61 gggcggcgcc gcaggtacta cgcgatcacc gtcgccgggc gggcggcact ggcggagcag
      121 cagcgggtag agcatgccgt cagtccagtc catctcgccg ccggagagct ggttgatccg
      181 tttgaggatg gcgtagccgt agctttcgcc ctcggccagg atcgccagca ccagcggcgt
      241 cgccgacgcc gccacgaggt ccttgtcgat gcgcatgttc cgcccctatg gctcggtgtg
      301 ccatgcatag gagcgcaagg tataggcggg cgccactgga cgaccggcca gtgcggaccg
      361 acgaggcgct ccacacaggg gctgcgagcc gcacccatgc ggcttcctga gtcatcggac
      421 cgctccccac gtcggaccgc ggcgacatcg attgtgcgaa gctggacgct cggtttagac
      481 tgccggtcta accgttctgg aggtccgttc gtgagcgatg cccctggatt ctcctcgcag
      541 atggcaatcg agcggttctt gcgggcgtca cagggcgagc cgttccagga cggcgagctg
      601 cttcccttcc acctgcccga gtgctacggc tgcgggcccg aaaacgagtc cggactccat
      661 ttgtcggtgc gggccgcgcc ggagaacgcc gtcgcaggcg agtacacctt cacccggcga
      721 ttgggcggtg cgccgggggt ggcccacggc ggtgcagtgg cggcgcttct cgacgacctg
      781 ttcgggtttg tgctcgtccg catccttgtg cccgcggtca cccgggacct gcaggtgcgc
      841 tatcgccggc cggtgcatct gcacacgccg tgtgcgctgt cagcgcgcct cgtcggcagg
      901 gaggggcgcg aagtgcacat ggccggggag ttgcggcagg aaggacaggt caaagccgca
      961 gcggaagcgg tcttcgtgga actcgagccg gagcggctgc tgaaccgcta cgagcgattg
     1021 gagccctgat gtcccggcag gtccccccgg atatcgctgg acgccttccg gccgcgctgc
     1081 acttgttcct ggaccagggc ctggacggca cgcgggtgga cgcgatcgtc gccgcgaccg
     1141 gcattccgaa ggcgacgctg tactaccact tcgacagcaa ggaggcgatc ttcgcgttcc
     1201 tgctccgcga gttgctcgac gagcttgcgg aggcggtcga cgccgccaag cgggggcggg
     1261 ggacggcgcg ggagcgcctc gtcgcggtga tcgaagcgat cgtcggcgcg atggcggcga
     1321 acccgacaag ctgcctggtc ctgctgagca acctgagccg cgccgggcga atgcccgaga
     1381 tcgcggggag cgtgcaggcg gcattccacg cgccggtcga ggagctgctc gtggaaggca
     1441 gcagggacgg gtcccttcgt gccacgggcc cggccacggc gagtgccgtg ttcggtgcgg
     1501 tcgtactcgt cagcctgcac cacctgatca tgaccggtga catccccgtg cgggccaccg
     1561 tggacagcgt cggcagcttg ctcctccacg ggctggcgac cggcgccgca gagggaaccg
     1621 atggctgagc gtctgctgtt caccgcgctg cggcatccga aggccgtggt gggtgcgtgg
     1681 ctgttggccg ccatcgtgct gctcgtgacc gcgccgtctc tgcgtgacgt cggcacgctg
     1741 gacaccgcag agctcctccc gagtacctct ccgagcgtcg tggccgctga agcgtcgcgc
     1801 cgcgcgtttc ccgaagatct cggacgcaac agcgccgtcg tgatcgtgcg gcgacccggc
     1861 gggctgctcg acaccgaccg cgcgtggctg gcgcagaccg ccgagcggct cgcggcccac
     1921 cctcttgccg ccgccactcg cgtgctgagc gcggcagcca accccgcaat gggcaccgct
     1981 ctgcgctccc cggacggagc ggccgagctg ctgctcgtgg agttggaggg cagcccgttc
     2041 gagccggaga ccgctgaggc gatcgaacag ctccgccaag tcgtacgggc ggacgcgccg
     2101 gcaggggtgg acgcggcggt caccggcatc gccgcgctcg gcgcggacca gggtgaggcg
     2161 atcaccgcgt cgatcgacca gaccgccgtg atcacgatcc tgctcatcct gctcatcctg
     2221 tggtgggtgt atcgcgcacc gctcgccgtg ctgctgcccc tggcgacgat cgcggtcgcc
     2281 ttcgtcgtgt cccgttcgct ggtggcgttc ctggcggcga gcgggttcac ggtggccgga
     2341 catgccgaga cgttcatggt cgtgctggtc ctcggcgccg gcaccgacta cacgctgttc
     2401 ctcgtgtccc gctacgccga agaactggca cagggacagc ccctgcccga cgcgctgcgc
     2461 cgcaccgtcc gcgtgatcgc cggggtgatc gccgccgcgg cggccacggt catcgtcggc
     2521 ttcctgtcga tgttgaccgc cgacttcggc atgtaccggt cgttcgggcc tgcgatcgcg
     2581 ctcgcgatcg cggtcacgct cctggtgggg ctgacgttca cgcccgcgct gctcgccctc
     2641 acgggcaagg cggccttctg gccgtccggg cccgtcaagg ccagccgcgc ccacgcggct
     2701 tccggcaggt gggaacgggt tgccacgctc gtccgccatc gaccgatcga ggtgctgctc
     2761 gcgggcgcga tccttttgct tgtgccctcc gccgggctcg cctggctgac accgtcgatg
     2821 gatctcgtgg gtgacctgcc accggccgcc cagtcgcgcc agggcttcga gatcctcgat
     2881 cagcacttcc ccgccggcgc gctcgcaccc gtgcaagtgg tcgtcgcgca cgaccgtccg
     2941 ctcaccgacg acaccgcctt cgcggccctg gacgacttga ccgaccgcct caacgagcaa
     3001 cccggcatcg cgctggcgcg ctccatcaca caacctctcg gcgcaccgct cacccccgac
     3061 accatcgggc ggctcaccgg cggaggcttc gacccgcggg cgctgggcct ggacccggac
     3121 gtcgtcgacc tcaccccgct cctgcaggcc atcgagcaac cgggcggact ccgcctcacc
     3181 tggtcgctgc tggaacgcta cccccagttc aacgacctgc tggccccgtt cctgagcgac
     3241 gatcgacaga ccaccaggtt gatcgtggag ctggacggca acccgttcgt ccccgagtcg
     3301 cttggcaaac tcgatgccct gccgcacgtc gtcgcggaca cgctgtccgg cacctcgctc
     3361 gccggcgccc aggtgcacct cgccggatcg ccggcctact accgggacgt ccaggacgtc
     3421 gccgcccgcg acctcgccct catcacggtc gtgctcatgc tcggcatctt cctggtgctg
     3481 gccgccctgc tgcgatctct cgtcgcgccg atctacctgc tcgccaccgt gctgctgtcc
     3541 tacgctgcca cgctgggcct cacgacatgg atgtcgatgc tggtccttgg aaccgccgac
     3601 atggcgttct ggatcccgcc gttccttctc gtgatcctcg tcgcgctcgg cgcggactac
     3661 aacatcttca tcatggggcg catccgcgag gaggtcgacg ccggcagcga catcggcgcg
     3721 gccacggccc gcgggctggt gctcaccggc ggcgtgatca cctcggcggg gctcatcctc
     3781 gccggcacct tcgccgtgct catgctcgcg cccatgccga accttcgcct gatcggcttc
     3841 gccgtcaccg tcggcgtgct gctcgacacc ttcgtcgtcc gttcggtgct cgtccccgcc
     3901 gccaccgtgc tgctggaccg ctgggcgctg tggccttccg cccacccgcg ggtgcgcgcc
     3961 ctgcgccttg ctccagccac catcgccggg ctggtcgtcc tgctgctggc cgccgccgcg
     4021 ctgcccagcc tgcccgccga acccgtggtc gtcgcccacg cggccgtcga gcaacagggc
     4081 gcaccggatc gtcaaccgcc cgcagacgac ccgagcgac
//
LOCUS       DATMKC010000327         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_66163, whole genome shotgun sequence.
ACCESSION   DATMKC010000327 DATMKC010000000
VERSION     DATMKC010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66163"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1932
                     /locus_tag="VNU01_09695"
     CDS             52..1932
                     /locus_tag="VNU01_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32930.1"
                     /translation="MSSLVGRDRAHRRTGAERAQHHPPPRPSASGAAAPEAGAPAGLR
                     ELLDDRVCLLRYLAERGVPHLPLEAVLAPDGSGWSWTTGRAVTPGGWALALDALGGAI
                     SVTPARRSEGGARMLWRRVPAGWRGTDGRTVGVQELAHHLRCLAPHRAWVVQRAPAAH
                     PLLAGAGEDPHLRIRTRVERSGGLHVAAAALVVGRRAADVLRLDTARGVPMAPPDRHL
                     PLAPSAALAAALLRTLRVPLWDEVVALSIRATEALAPLGGVWLGVSVEPGGPVVRDVW
                     GDPPRTRTLRGPAASPRPRPLADFGELRRAARDVYGAATLPVLARAARLRHRRGLPLR
                     ESLRRGLLEPGRRLDPDVVPQAARIRLQAQLNAAGGHALADDRALLALLGEAAALPVP
                     PLRALLPPGGWGWCCRQDAPVHPDGWEQALAALPGDLIVRPHAGVRGAVAARRAGGAW
                     WPDGDAAALVARLAADRTVPLWVVQALPAPHPVLAAATDGGLCSLHVITLMRSRGRVE
                     LLRAWLGSAGDVTGLVAEVDPRDGRVAAARRAGRGGYGLRPADPDVAGLRELRGRCLP
                     GWAGVEEVAARTARALLPLRTLGLTLALTSAGPVLLEADPFWEPVAGEDLRPALARME
                     AA"
     gene            1936..3402
                     /locus_tag="VNU01_09700"
     CDS             1936..3402
                     /locus_tag="VNU01_09700"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWH32931.1"
                     /translation="MGARRPENTGRRVAGESLPHFGPTTDLVTRLQRALDHERAARRE
                     AEGRLERERRRHRRDELTGLPTRPAMLERIDAVAGRAGRTPYALLCADIDAFARINDT
                     LGHEVGDRVLRHVAARIRAVAPGGDMVGRLGDDAFVLVCEGMDTADAIALADRIREEV
                     GRGVEVGALRVTPTVSIGVRVVTGRGAGPANALRDAEVAAQRAREAGRGRLEVFDEGV
                     HAEIRERLRLERRLEQALDEGSLSLAYQPIVHPVTLQPMGYEALLRWDDPELGSVPPA
                     RFIPVAERTRQIVAIGEWVLTRALEDMDRAFGGAGRPRQIAVNVSPVQLHEPAFATHV
                     REALERFDVAPQRLVLEVTESLLVSDDEVVAHNLARLRQLGLALAIDDFGVGYSSLSK
                     LSRLKAGILKIDRSFVSDISPASDGRLIVAAIIGLARGLGIATIAEGVERAEQLDALR
                     DMGIDRVQGFLLGRPAADPRPVAGTGGALASYLAATPA"
BASE COUNT          319 a         1325 c         1364 g          409 t
ORIGIN      
        1 acgttagggc gaagttaggt tgcgccctgt ccccgagtag gaatgtgccc catgtcctcg
       61 ctcgtcggcc gcgaccgcgc gcatcgtcgc acgggcgcgg agcgcgcgca gcaccatccg
      121 cccccccgcc cgtccgcctc cggcgcggcc gcgccggagg cgggtgctcc ggccggcctg
      181 cgggagctcc tcgacgaccg ggtctgcctg ctgcgctacc tcgccgagcg gggcgtgccg
      241 cacctgcctc tggaggccgt gctcgctccc gacggctcgg gctggagctg gacgaccggg
      301 cgcgccgtca cccctggggg ctgggcgctc gccctggacg cgctcggggg cgccatctcg
      361 gtcaccccgg cgcgccggtc cgagggcggg gcgcggatgc tgtggcgccg cgtgccggcc
      421 ggctggcgcg gcaccgacgg gcgcacggtg ggcgtgcagg agctggccca ccacctgcgc
      481 tgcctggccc cgcaccgcgc ctgggtggtg cagcgggcgc ccgccgccca ccccctgctg
      541 gcgggtgccg gcgaggatcc ccacctgcgc atccggacgc gcgtggaacg ctcgggcggc
      601 ctgcacgtcg cggccgccgc cctggtcgtg ggacggcgcg ccgccgacgt gctgcgtctg
      661 gacaccgcgc gcggggtgcc catggccccg cccgaccgcc acctgcccct ggcgccctcc
      721 gcggcgctcg cggcggcgct gctgcggacg ctgcgcgtgc cgctgtggga cgaggtggtc
      781 gcgctgtcga tccgcgccac ggaggccctc gcgcccctgg gcggcgtgtg gctgggcgtg
      841 tcggtggaac cgggcgggcc ggtggtgcgc gacgtgtggg gcgacccccc gcgcacgcgg
      901 accctccgcg ggcccgccgc gtccccgcgc ccccggccgc tggccgactt cggcgagctc
      961 cgccgagccg cgcgcgacgt gtacggcgcc gccacgctgc ccgtgctcgc ccgcgccgcc
     1021 cgcctgcggc accggcgggg actgccgttg cgcgagagcc tgcggcgcgg gctgctggag
     1081 ccgggccgcc gcctcgaccc ggacgtcgtg cctcaggccg cccgcatccg cctgcaggcg
     1141 cagctcaacg ccgcgggcgg gcacgccctc gccgacgacc gcgcgctgct ggcgctgctg
     1201 ggcgaggcgg ccgcgttgcc ggtgccgccg ctgcgcgccc tgctgccgcc gggtggctgg
     1261 ggctggtgct gccgccagga cgcccccgtc cacccggacg gatgggagca ggcgctcgcc
     1321 gcgctgcccg gggacctgat cgtgcgtccg cacgcgggcg tgcgcggcgc cgtcgccgcc
     1381 cgccgcgccg gcggggcctg gtggcccgac ggcgacgcgg cggcgctggt cgcgcgcctg
     1441 gccgccgacc ggacggtgcc gctgtgggtg gtgcaggcgc tgcccgcgcc gcaccccgtg
     1501 ctcgcggcgg ccacggacgg cgggctctgt tcgctccacg tgataacgct gatgcgctca
     1561 cgcggccgtg tggagctgct gcgcgcctgg ctcggctcgg cgggcgacgt gaccggcctc
     1621 gtcgccgagg tggacccccg cgacggacgg gtggccgcgg cacgccgggc gggccggggc
     1681 ggctacggcc tgcggccggc ggacccggat gtcgccgggc tgcgcgagct gcgcggccgg
     1741 tgcctgcccg gctgggcggg cgtcgaggag gtcgcggcgc gcaccgcccg ggcgctgctg
     1801 ccgctgcgca cgcttgggct gacgctggcg ctgacctcgg cggggccggt gctgctggag
     1861 gccgacccct tctgggagcc ggtcgccggc gaggacctgc gccccgcgct ggcgcgcatg
     1921 gaggcggcgt gatcgatggg cgcgcgccgt cccgagaaca cgggtcgccg cgtggccggt
     1981 gagagcctcc cccacttcgg tcccaccacc gacctggtca cgcgcctgca gcgggcgctc
     2041 gaccacgagc gggccgcgcg ccgggaggcc gagggccgcc tggagcgcga gcgccgccgg
     2101 caccgccgcg acgagctgac cggcctgcct acgcgcccgg cgatgctgga gcgcatcgac
     2161 gcggtggccg gccgggccgg ccgcaccccc tacgccctgc tgtgcgccga catcgacgcc
     2221 ttcgcccgca tcaacgacac cctgggccac gaggtcggcg accgcgtcct gcgccacgtg
     2281 gccgcgcgga tccgcgcggt cgcgcccggc ggcgacatgg tgggccggct gggcgacgac
     2341 gccttcgtgc tggtgtgcga ggggatggac acggccgacg cgatcgccct cgcggaccgc
     2401 atccgcgagg aggtgggccg cggggtcgag gtgggcgcgc tgcgggtcac gcccaccgtc
     2461 agcatcggcg tgcgcgtcgt caccggccgc ggcgccgggc ccgccaacgc cctgcgggac
     2521 gccgaggtgg cggcgcagcg ggcccgcgag gcgggccggg gccggctgga ggtgttcgac
     2581 gagggcgtgc acgccgagat ccgcgagcgc ctgcggctgg agcggcggct ggagcaggcg
     2641 ctcgacgagg ggagcctgtc gctggcgtac cagccgatcg tgcacccggt caccctgcag
     2701 ccgatgggct acgaggccct gctgcggtgg gacgatccgg aactcggaag cgtgcccccc
     2761 gcgcgcttca tccccgtggc cgagcgcacc cggcagatcg tggccatcgg cgagtgggtg
     2821 ctgacgcgcg ccctggagga catggaccgg gccttcggcg gtgcgggacg gccccgtcag
     2881 atcgcggtga acgtgtcgcc cgtgcagctg cacgagcccg cgttcgcgac ccacgtgcgg
     2941 gaggcgctgg agcgcttcga cgtggcgccc cagcggctgg tgctggaggt gaccgagtcg
     3001 ctgctggtct ccgacgacga ggtcgtggcc cacaacctgg cccggctgcg gcagctgggg
     3061 ctggccctgg cgatcgacga cttcggcgtc ggctactcgt cgctgtccaa gctgagccgg
     3121 ctgaaggccg gcatcctgaa gatcgaccgc tcgttcgtgt ccgacatctc gcccgccagc
     3181 gacggacgcc tgatcgtcgc cgccatcatc ggcctggcgc gcggcctggg catcgccacc
     3241 atcgccgagg gcgtcgagcg cgccgagcag ctggacgcgc tgcgcgacat gggcatcgac
     3301 cgggtgcagg gcttcctgct cggccgcccc gcggccgacc cgcgtcccgt cgccgggacg
     3361 gggggcgcgc tcgcgtcgta cctcgccgcg acgcccgcct gagcggggcc cgggccc
//
LOCUS       DATMKC010000328         6334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_33005, whole genome shotgun sequence.
ACCESSION   DATMKC010000328 DATMKC010000000
VERSION     DATMKC010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33005"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..118)
                     /locus_tag="VNU01_09705"
                     /pseudo
     CDS             complement(1..118)
                     /locus_tag="VNU01_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            131..1138
                     /locus_tag="VNU01_09710"
     CDS             131..1138
                     /locus_tag="VNU01_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH32932.1"
                     /translation="MLGEIDGFEGSEGVVVMAATNRPDVLDPALLRPGRFDRQIVVPL
                     PTLSERVEILNVHTKGKPIAEDADLRTLARGTPGMSGADLKNLVNEAALIAVRSGSDD
                     IRMRDLEQARERHTIGRERSSLRLDEEEKRAVAYHESGHALAAFLSDEADPVYKVTVL
                     PVGMALGVTTQLPIDERHIYLRSYLDAKIKVMLGGRAAEMAVLGQPTTGGQHDLVQAT
                     RLARAIVREFGMSELLGPVGFGDERQVFLGEELTRGHEYSEQTAQLIDQEVRRMLAVA
                     LEEVVEEFARLRAALDAMADALVERESLSGEEALAIAREALPARTRARLRGRAAAAMA
                     S"
     gene            1150..1803
                     /gene="npdG"
                     /locus_tag="VNU01_09715"
     CDS             1150..1803
                     /gene="npdG"
                     /locus_tag="VNU01_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWH32933.1"
                     /translation="MSEQILGFLGGTGPQGRGLAARFAAAGLPVLLGSRDADKGRGAA
                     AELGQAHGLRIDGGANADAAAAADVVFVVVPYAAQADTVAPLAEALRGKVVVHCVNHM
                     LFDERGPYAPGVPGGSAAEECAALLPGANVVSAFHDISARALAKVGEPMDADVLVCGD
                     EAGAKEAVIALAARIPGLRGVDCGPLRMSRAIEDLTPVLVNINRRYKTHASLKIAGL"
     gene            complement(1800..2171)
                     /locus_tag="VNU01_09720"
     CDS             complement(1800..2171)
                     /locus_tag="VNU01_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32934.1"
                     /translation="MPVAQTNRDRLRGGLLGPLGEETTDMLMDALDQRGLRDHVDERF
                     DEQRRYVDQRFEQVDKRFEQVDKRFEQIEDRLARIDDAVRMLALAVASSSRPANTIVY
                     TVCGTIAVVAIATFLESVLAG"
     gene            complement(2213..3541)
                     /locus_tag="VNU01_09725"
     CDS             complement(2213..3541)
                     /locus_tag="VNU01_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HWH32935.1"
                     /translation="MSRTSAPLLERHPGEADLLGLAAASPGFAQLHTVRGWTVALCDP
                     LDVVTDLAELSAAASRLGWRAPRDPALPPFTAGLAGFLTDTLSAGLLDLAEPAGRPAP
                     APLPPLAFGLYDTAVLRDPDGEVWLAAGELPGYSTVPAAQRLAGLRERVARAAPPPDG
                     AAAPPPARVTASLSRDGHAKAVRQAQEWIAAGDLYQLNLTLQLRAPWGLGGAALARRL
                     WQATPGAAHAAWLRAPGGTEVVSASPETFLRVDGDRAVVRPIKGTRPRHADPRRDHAE
                     RLALEASVKDRAEHVMIVDLERNDLGRVAVAGGVTVEALAATEALPTLWHLVSTVSAR
                     LRPEVSLEDLLAAAFPCGSVTGAPKRMAVARIGALEPVQRGVYCGATGVIGPGLVDLS
                     VAIRTAVVHDGQASYGTGGGIVADSDPDAEWQEVLHKAEAFFSAANARLP"
     gene            complement(3558..3794)
                     /locus_tag="VNU01_09730"
     CDS             complement(3558..3794)
                     /locus_tag="VNU01_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32936.1"
                     /translation="MTEELARRRFRVLTRTRGGYNGSTMYDVQLQSVESGQLLWARTF
                     TDREQAEEFQASLDADLDMEDGEFRRKHGVPSGA"
     gene            4033..4575
                     /locus_tag="VNU01_09735"
     CDS             4033..4575
                     /locus_tag="VNU01_09735"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWH32937.1"
                     /translation="MKVYTRKGDDGTTGLLYGGRVHKDDLRTDAYGTTDEACSALGMA
                     RAELAGGEWHDEILRVQRELFVVGAQLATHVDHWPQLDGRVSRVVPEMVDELEARIDA
                     LTERYPLPKEFTVPGGSRGGAAIDLARTIVRRAERMAVRMAREGLLPDETPLRYLNRL
                     SDYLFVLARAVEGGAHTPTR"
     gene            4589..5305
                     /locus_tag="VNU01_09740"
     CDS             4589..5305
                     /locus_tag="VNU01_09740"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HWH32938.1"
                     /translation="MSLVPSVTEAVAAMGAGEHLVGVTDWCAVGAPAHAARVGGTKSP
                     SLAKIAALRPDVVVVNQEENRREDAEALGAAGIEVLVTFPRTVADVPPMLRELGALVR
                     RASQADAFACDLEEALAEPVPDAAVPVLTLIWRKPWMAVGAGTFADDLLHLAGFRNVL
                     TDRSTPYPKVQALSAAPDVVLLPTEPYEFGEKDLSAVRELVGERPALEFVDGQLLTWH
                     GVRTATALRHFRAVARRLNQ"
     gene            complement(5299..6144)
                     /gene="panB"
                     /locus_tag="VNU01_09745"
     CDS             complement(5299..6144)
                     /gene="panB"
                     /locus_tag="VNU01_09745"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWH32939.1"
                     /translation="MSVHSSPGKPSGSHGAPQPLSIHDLKSFKQRGERFTMLTAYDFL
                     TAQILDEAGVPVLLVGDSLGMVMLGYDSTVPVTMDEMLHHTRAVRRGASRALVVGDMP
                     FMSYQGSTDEAIRNAGRFLKEGGANAVKLEGGGPVLDLVARLVSIGIPVMGHLGLTPQ
                     SVNQFGGFKVQGRAADDAERILDDAIALADAGAFSIVLECVPAELAARVTEAVPVPTI
                     GIGAGAATDGQVLVIQDLLGLTAGRLPRFVKQYANLRGQIADAAKGFQHEVASGDYPG
                     PEHAY"
BASE COUNT          843 a         2236 c         2428 g          827 t
ORIGIN      
        1 tcttgaacag atcgcgcacg cggctggcgc cgacgccgac gaacatctcc atgaagtccg
       61 agccggtcac ggagatgaac ggcacgccgg cctcgccggc cacggcgagc gcgagcagac
      121 cctcaaccag atgctcggcg agatcgacgg cttcgagggc agcgagggcg tcgtggtcat
      181 ggcggcgacc aaccgcccgg acgtgctgga cccggcgctg ctgcgtcccg gccgcttcga
      241 ccggcagatc gtggtgccgc tgccgaccct gtccgagcgc gtcgagatcc tcaacgtgca
      301 caccaagggc aagccgatcg ccgaggacgc cgacctgcgc acgctggcgc gcggcacgcc
      361 cgggatgagc ggcgcggacc tgaagaacct ggtgaacgag gcggcgctca tcgccgtgcg
      421 ctccggcagc gacgacatcc gcatgcgcga cctggagcag gcgcgcgagc gccacaccat
      481 cggccgcgag cgctccagcc tgcgcctgga cgaggaggag aagcgcgccg tcgcctacca
      541 cgagagcggc cacgcgctgg cggcgttcct gtcggacgag gccgaccccg tctacaaggt
      601 gaccgtgctg ccggtgggca tggcgctggg cgtcaccacg cagctgccga tcgacgagcg
      661 gcacatctac ctgcgcagct acctggacgc caagatcaag gtgatgctgg gcggccgcgc
      721 cgccgagatg gcggtcctgg ggcagcccac caccggcggc cagcacgacc tggtgcaggc
      781 gacgcggctg gcgcgcgcca tcgtgcgcga gttcggcatg agcgagctgc tggggccggt
      841 gggcttcggc gacgagcgcc aggtcttcct gggggaggag ctcacgcgcg ggcacgagta
      901 cagcgagcag accgcgcagc tcatcgacca ggaggtccgg cgcatgctcg cggtggcgct
      961 ggaggaggtc gtcgaggagt tcgcgcgcct gcgcgcggca ctggacgcca tggcggacgc
     1021 gctggtggag cgcgagtcgc tgtccggcga ggaggcgctg gcgatcgcgc gggaggcgct
     1081 gcccgcacgg acgcgcgccc ggctgcgcgg ccgggcggcc gcggcgatgg ccagctagcc
     1141 tgctcgcgca tgagcgagca gatcctgggc ttcctgggcg gcaccggccc gcagggcagg
     1201 gggctggccg cgcgcttcgc cgccgcgggc ctgcccgtgc tgctcggctc ccgcgacgcg
     1261 gacaaggggc gcggggcggc ggccgagctc ggccaggccc atggcctccg aatcgacggc
     1321 ggcgccaacg ccgacgcggc ggcggccgcc gacgtggtgt tcgtggtggt gccctacgcc
     1381 gcgcaggcgg acaccgtggc gccgctggcc gaggcgctgc gcggcaaggt ggtggtgcac
     1441 tgcgtgaacc acatgctgtt cgacgagcgc ggcccgtacg cgcccggcgt gccgggcggc
     1501 tcggcggcgg aggagtgcgc ggcactgctg ccgggcgcga acgtggtgag cgcgttccac
     1561 gacatctccg cgcgggcgct ggcgaaggtg ggtgagccga tggacgccga cgtgctggtg
     1621 tgcggggacg aggcgggtgc caaggaggcg gtgatcgcgc tggcggcgcg catcccgggc
     1681 ctgcgcggcg tcgactgcgg cccgctgcgc atgtcccgcg cgatcgagga tctcacgccc
     1741 gtgctggtca acatcaacag gcgttacaag acgcacgcga gcctgaagat cgccggcctc
     1801 tagcctgcca ggacgctctc caggaacgtg gcgatcgcca cgaccgcgat ggtgccgcac
     1861 acggtgtaga caatggtgtt cgcggggcgg gacgaactcg cgacggcgag cgcgagcatg
     1921 cggaccgcat cgtcgatgcg cgcgaggcga tcctcgatct gctcgaaccg cttgtcgacc
     1981 tgctcgaacc gcttgtcgac ttgctcgaac cgctggtcga cgtagcggcg ctgttcgtcg
     2041 aaccgctcgt cgacgtgatc gcggagcccg cgctggtcca gcgcgtccat cagcatgtcg
     2101 gtcgtctcct cgccgagcgg cccgagcagt ccgccgcgga gccggtctcg attcgtctgc
     2161 gccacgggca ttgtcgacct ccccggttcg atgggcaacc attcggccga cattacggca
     2221 ggcgcgcgtt ggccgccgag aaaaaggcct ctgccttgtg gagaacttcc tgccactccg
     2281 cgtccgggtc ggagtcggcc acgatgccgc cgcccgtgcc gtacgaggcc tgcccgtcgt
     2341 gcacgacggc cgtgcggatc gccacggaca ggtccaccag ccccgggccg atcacgccgg
     2401 ttgccccgca gtacacgccc cgctggaccg gctccagggc gccgatgcgc gccaccgcca
     2461 tgcgcttggg cgcgccggtc accgacccgc acgggaacgc cgcggccagc aggtcctcca
     2521 gcgacacctc cgggcgcagg cgcgccgaca cggtggacac gaggtgccac agcgtcggca
     2581 gcgcctcggt ggccgccagc gcctcgaccg tgacgccgcc cgccacggcg acgcgcccca
     2641 ggtcgttgcg ctccaggtcc acgatcatga cgtgctccgc gcggtccttg accgacgcct
     2701 ccagcgccag ccgctcggcg tggtctcgcc gggggtcggc gtgccggggg cgggtgccct
     2761 tgatgggccg caccaccgcg cggtcgccat cgacgcgcag gaacgtctcc ggcgaggccg
     2821 acacgacctc tgtgccgccg ggcgcgcgca gccacgccgc gtgtgccgct ccgggcgtgg
     2881 cctgccacag gcggcgcgcc agcgccgccc cgcccaggcc ccagggcgcg cgcagctgca
     2941 gggtcaggtt cagctggtac aggtcgccgg cggcgatcca ctcctgcgcc tggcgcaccg
     3001 ccttggcgtg cccgtcgcgg ctgagcgacg ccgtcacgcg ggccggcggc ggcgccgcgg
     3061 caccgtcggg cggtggcgcc gcccgtgcca cccgctcgcg caggccggcc aggcgctggg
     3121 cggcgggcac ggtcgagtag ccgggcagct cgcccgcggc cagccacacc tcgccgtccg
     3181 ggtcgcgcag caccgcggtg tcgtacagcc cgaacgccag tggcggcagc ggcgcgggcg
     3241 cgggccggcc ggcgggctct gccaggtcga gcagcccggc ggacagcgtg tcggtgagga
     3301 agccggcgag cccggccgtg aacggcggca gcgccgggtc gcgcggcgcg cgccagccca
     3361 gccgggaggc cgcggcggac agctcggcca ggtccgtgac gacgtccaac gggtcgcaca
     3421 gcgcgaccgt ccagccgcgg acggtgtgga gctgggcgaa gccgggcgac gcggccgcca
     3481 gtcccagcag gtcggcctcg ccgggatggc gctccagcag cggggcgctc gtgcggctca
     3541 ccgcggcgcc gccggggtca ggcgcccgac gggacgccgt gcttgcggcg gaactcgccg
     3601 tcctccatgt ccaggtccgc gtccagggag gcctggaact cctccgcctg ctcgcggtcg
     3661 gtgaacgtgc gcgcccacag cagctggccc gactccacgg actgcagttg gacgtcgtac
     3721 atggtggagc cgttgtagcc cccgcgggtc cgggtcagca cccggaacct gcgtcgtgcg
     3781 agctcttcgg tcacgtctgc tacagcccct gctccatggg tcggccgcgt gtcggccttt
     3841 tgcctccggg accggtcgcg atgccggtgg cccagctccc gcacggcggc gggacgcatg
     3901 gtccggcggc aggccacgat ggacgtggga gccgggtctt ggagggaggt gacggtaccc
     3961 gaccggtacg cgtggccgca acgcggcggg acgggcgccg gggacggccg gggggctgac
     4021 tagactggcc gcatgaaggt ctacacccgc aagggcgacg acgggacgac cgggctgctg
     4081 tacggcggca gggtgcacaa ggacgatctt cgcaccgacg cgtacgggac cacggacgag
     4141 gcctgctcgg cgctcggcat ggcgcgggcc gagctcgccg gcggcgagtg gcacgacgag
     4201 atcctgcgcg tccagcggga gctgttcgtg gtgggcgcgc agctggccac gcatgtcgac
     4261 cactggccgc agctcgacgg tcgcgtgtcg agggtcgtgc cggagatggt ggacgagctc
     4321 gaggcgcgca tcgacgcgct gaccgagcgt tacccgctgc ccaaggagtt caccgttccc
     4381 ggcggctcgc gcggcggcgc ggcgatcgat ctcgcgagga ccatcgtgcg gcgcgccgag
     4441 cgcatggccg tgcgcatggc gcgtgagggc ctgctgccgg acgagacgcc gctgcgctac
     4501 ctgaaccggc tgtcggacta cctgttcgtg cttgcgcggg cggtcgaggg cggcgcccac
     4561 acgccgacgc ggtgacgccg tgcggctggt gagcctggtc cccagcgtca ccgaggccgt
     4621 cgcggcgatg ggcgcggggg agcacctggt cggcgtgacc gactggtgcg cggtgggcgc
     4681 gccggcgcac gcggcgcgcg tcggcggcac gaagagcccc tcgctggcca agatcgccgc
     4741 cctgcggccg gacgtggtgg tggtgaacca ggaggagaac cgccgcgagg atgccgaggc
     4801 gctgggcgcg gccggcatcg aggtgctggt gacgttcccc cggacggtcg cggacgtgcc
     4861 gccgatgctg cgcgagctcg gcgcgctggt gcgccgagcg tcacaggcgg acgcgttcgc
     4921 gtgcgatctg gaggaggcgc tcgccgaacc ggtccccgac gcggccgtgc ccgtgctcac
     4981 gctgatctgg cgcaagccgt ggatggccgt cggtgccggc acgttcgccg acgacctgct
     5041 gcacctggcc ggcttccgca acgtcctgac tgaccgctcc acgccctacc ccaaggtgca
     5101 ggcgctctcc gcggcaccgg acgtcgtgct gctgccgacc gagccgtacg agttcgggga
     5161 gaaggacctg tccgccgtgc gcgagctggt gggcgagcgg ccggcgctgg agttcgtcga
     5221 cggacagctg ctgacctggc acggcgtgcg cacggccacc gccctccgcc acttccgcgc
     5281 cgtggcgcgc cgcctgaatc agtaggcgtg ctcggggccg ggatagtcgc cggacgcgac
     5341 ctcgtgctgg aagcccttgg cggcgtcggc gatctggccg cgcaggttcg cgtactgctt
     5401 cacgaagcgc ggcaggcgcc cggcggtcag ccccaggagg tcctggatca ccagcacctg
     5461 cccgtccgtg gcggctccgg cgccgatgcc gatggtcggc acgggcacgg cctcggtcac
     5521 gcgggcggcc agctcggccg gcacgcactc cagcacgatg ctgaacgcgc cggcgtcggc
     5581 cagggcgatc gcgtcgtcca ggatgcgctc ggcgtcgtcg gccgcgcggc cctgcacctt
     5641 gaagccgccg aactggttga cgctctgcgg cgtcagcccc aggtggccca tgacggggat
     5701 gccgatggac accaggcggg cgaccaggtc cagcacgggg ccgccgccct ccagcttcac
     5761 ggcgttggcg ccgccttcct tgaggaagcg gccggcgttg cggatggcct cgtccgtgga
     5821 gccctggtag ctcatgaacg gcatgtcgcc caccaccagc gcgcgcgagg cgccgcggcg
     5881 caccgcccgg gtgtggtgga gcatctcgtc catggtcacc ggcacggtgg agtcgtagcc
     5941 gagcatcacc atgccgagtg agtcgccgac cagaagaacc ggtactcccg cttcgtccag
     6001 tatctgtgcg gtgagaaagt cgtacgcggt gagcatggtg aaccgctcgc cgcgctgctt
     6061 gaaggacttc aggtcatgga tggaaagcgg ttgcggagca ccgtggctcc ccgacggctt
     6121 cccgggtgag gaatgcacgc tcatgtgatc acgctccctg ccggcccgac tgctggggac
     6181 gcagtctcgg atgggtccag cggtgtgacg cgagtgtagg acaggaggct gtctgatgca
     6241 ggtcgtggtg cagcgggtcc agcgggccgc ggtggcggtg gacgacgcca tcgtgggcga
     6301 ggtggggcag ggcctgttcg cgctggtcgg cgtg
//
LOCUS       DATMKC010000329         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_149780, whole genome shotgun sequence.
ACCESSION   DATMKC010000329 DATMKC010000000
VERSION     DATMKC010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149780"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VNU01_09750"
     CDS             <1..912
                     /locus_tag="VNU01_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWH32940.1"
                     /translation="VLWEHTTDVRHGGARTLLSADGVVVATGGSGGATAFDAESGEAL
                     WQAPAAGEGYLPAVALVPAPQAAVVMLERDGALVSRDLRSGRERWRAQPGGAHRMGLA
                     AGGGVVAVLVADEGANGPQAGTVAVFDSADGSPRWERTVPERLGALLAVEGTVLAHGE
                     IVRYAWDAATGDQRWRVPRIGPFARLAATAQGPAAVTQDEIVLFDAVTSYEHRRVAAG
                     NALPDSVRVEADRLLLSTRDGHAEVRALDGEVLRRLPLGGAGGRPAAGAGLLAVPTHI
                     GVSAFGEDGALRWWHLRELPEERASGG"
     gene            complement(919..1362)
                     /locus_tag="VNU01_09755"
     CDS             complement(919..1362)
                     /locus_tag="VNU01_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWH32941.1"
                     /translation="MTAWCLTTSPGNFAKTAELGWKVQGIKSRRRPTAMQIRPGDKLV
                     YYITEVVAFGGIAEVTSECFEDHELIWTSKPGEDYPWRVEIAPEIVIEQRERWVAADE
                     LRDALEFPRKWPPENWKLAFQGNIRAWPDTDYETVRNALRKAATR"
     gene            complement(1359..2018)
                     /locus_tag="VNU01_09760"
     CDS             complement(1359..2018)
                     /locus_tag="VNU01_09760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWH32942.1"
                     /translation="MRVHIDEQSLSYLRRFTQQEDEALRAARARSEEADIPAVAPETG
                     ALLRWFAHLLNARHVAEVGTGGGYSGLWLLGGMDPRGTLTSVDADPDHHALAQRAFGD
                     AGLLDRVRLMTGAALQVLPKLADHHYDIVFLDAAKSEYPAYLEHARRLLRPGGVLIAD
                     NVLWHGKVADPLEGDDDTSGIREFNDQVREDPTLLPVLLPVGDGVLCAVYQPLEQPEQ
                     P"
     gene            2178..>2740
                     /locus_tag="VNU01_09765"
     CDS             2178..>2740
                     /locus_tag="VNU01_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWH32943.1"
                     /translation="MSDWSRPDTAVPPPPEPVYPHIPAGPEPRQETAGALTEPVPAPS
                     TGARLGVVAVVAALVGALAGGGAALVATTDLREQPAAAPAPERGEDVRTEPLQTGNSG
                     DLSRVAAVAERVLPSVVQIDVGRTGPVGGAAGNGSGVVYRSDGYIVTNNHVVAGGRQI
                     EVVLPDGSRLPARVIGSDPDNDLAVVKV"
BASE COUNT          331 a          995 c         1052 g          362 t
ORIGIN      
        1 gtcctgtggg agcacacgac ggatgtccgg cacggcggcg cgcgcacgct gctgtccgcc
       61 gacggcgtcg tggtcgccac cgggggctcg ggcggagcca ccgcattcga cgcggagtcg
      121 ggcgaggcgc tgtggcaggc ccccgctgcc ggcgaggggt atctgcccgc tgtcgcgctg
      181 gtgccggcgc cacaggcggc cgtggtcatg ctggagcgcg acggcgcgct ggtctccagg
      241 gacctgcggt cgggccggga gcgctggcgc gcccagccgg gtggcgccca ccgcatggga
      301 cttgccgccg gcggcggtgt ggtcgcggtg ctggtcgccg acgagggcgc caacgggccg
      361 caggccggca ccgtcgcggt tttcgactcc gccgacgggt cgccgcgctg ggaacgcacc
      421 gtcccggagc gactcggcgc attgctcgcg gtggagggca cggtgctcgc ccacggcgag
      481 atcgtgcgct acgcgtggga cgccgcgacc ggcgaccagc gctggcgcgt gccgcggatc
      541 ggcccgttcg cccggctggc cgcgacggcg cagggccccg cggcggtgac acaggacgag
      601 atcgtgctgt tcgacgcggt cacgtcctac gagcaccggc gcgtggccgc cgggaacgcg
      661 ctgcccgatt cggtccgcgt ggaggccgac cggctgctgc tgtccacgcg tgacggccac
      721 gccgaggtgc gcgcgctgga cggcgaggtg ctgcggcgcc tgcccctggg tggcgcgggt
      781 ggccgccccg ccgccggcgc cggcctgctg gcggtgccca cgcacatcgg cgtgtcggcg
      841 ttcggcgagg acggggccct gcgctggtgg cacctgcggg agctgccgga ggagcgggcc
      901 agcggcggct gatcccggtc agcgcgtggc ggccttgcgc agcgcgttgc gcaccgtctc
      961 gtagtcggtg tccggccagg cgcggatgtt gccctggaac gcgagcttcc agttctccgg
     1021 cggccacttg cgcgggaact ccagcgcgtc gcgcagctcg tccgcggcga cccagcgctc
     1081 gcgctgttcg atcacgatct ccggcgcgat ctcgacgcgc cacgggtagt cctcacccgg
     1141 cttggaggtc cagatcagct cgtggtcctc gaagcactcc gaggtcacct cggcgatgcc
     1201 gccgaacgcc accacctcgg tgatgtagta gacgagcttg tcgccggggc ggatctgcat
     1261 cgccgtcggc cggcgccgcg acttgatgcc ctggaccttc cagcccagct ccgcggtctt
     1321 ggcgaaattg ccgggcgagg tggtcaggca ccaggccgtc acggctgctc cggctgttcc
     1381 agcggctggt acaccgcgca caacacgccg tcgcccaccg gcagcagcac cggcaaaagc
     1441 gtcgggtcct cgcgcacctg gtcgttgaac tcgcggatgc ccgacgtgtc gtcgtcgccc
     1501 tccaacgggt ccgcgacctt gccgtgccag agcacgttgt cggcgatcag cacgccgccg
     1561 gggcgcagca gccgccgcgc gtgctccagg tacgccgggt actccgactt ggcggcgtcc
     1621 aggaacacga tgtcgtagtg gtggtcggcc agcttcggca gcacctgcag ggccgcgccg
     1681 gtcatcagcc gcacgcggtc gagcaggccg gcatcgccga aggcgcgctg ggccagggcg
     1741 tggtggtcgg ggtcggcgtc cacgctggtg agcgtgccgc gcggatccat gccgcccagc
     1801 agccacaagc cgctgtagcc gccgccggtg ccgacctcgg cgacgtggcg cgcgttgagc
     1861 aggtgcgcga accagcgcag cagcgcgccg gtctccgggg cgaccgccgg gatgtccgcc
     1921 tcctcgctgc gggcacgcgc cgcgcggagg gcttcgtcct cctgctgcgt gaaacgccgc
     1981 aggtaggaca ggctctgctc gtcgatgtgc accctcatgc tcctctcagg tcggcgcgga
     2041 aaggtaacca cgtcgcgacg tgcggcgaaa ccacccgcca cggcggccgc cggcggagcc
     2101 ggtgcacgcg acggctttgg ctgggcgccg cggcgcggcg gatacttgtc cgcgccgccc
     2161 gaggaacgag gatggacatg agcgactgga gccgacccga caccgcggtg ccgccaccgc
     2221 cggagccggt gtacccgcac attcccgcag gcccggagcc tcggcaggag accgcgggcg
     2281 ccctgactga gcccgtccct gcgccgtcaa cgggcgcgcg cctcggcgtg gtcgccgtgg
     2341 tcgccgccct ggtgggcgcg ctggccggcg gcggcgccgc cctggtcgcg acgaccgacc
     2401 tgcgcgagca gccggcggcc gcgccggcgc ccgaacgcgg cgaggacgtg cgcaccgagc
     2461 cgttgcagac cggcaacagc ggcgatctga gccgcgtggc cgccgtggcc gagcgcgtgc
     2521 tgcccagcgt cgtgcagatc gacgtgggcc gcacggggcc ggtgggcggc gcggccggca
     2581 acggctcggg cgtcgtctac cgcagcgacg gctacatcgt cacgaacaac cacgtggtcg
     2641 ccgggggccg ccagatcgag gtggtgctgc cggacggctc gcgcctgccc gcgcgcgtca
     2701 tcggcagcga cccggacaac gatctggccg tggtgaaggt
//
LOCUS       DATMKC010000330         4236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_120142, whole genome shotgun sequence.
ACCESSION   DATMKC010000330 DATMKC010000000
VERSION     DATMKC010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120142"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VNU01_09770"
     CDS             complement(<1..626)
                     /locus_tag="VNU01_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32944.1"
                     /translation="MLRALGDLLLPRRCGGCLAPVLGDGLWLCPACRAEISTLALPDA
                     GWSRPAPGVLAVAGFAYGGALAAAIRRAKTPGGHGLAPTLAVLLWERFGVGPAELPAV
                     RTWVPSVPARARSRAVELPRVLAGPGARPLLARRGAPPDQAGLAPARRRTAPFGTFVA
                     RGAVPPVVALVDDVRTTGATALALAAAGAVRVLVLTLAAGGQREHQDA"
     gene            complement(876..1721)
                     /gene="hisG"
                     /locus_tag="VNU01_09775"
     CDS             complement(876..1721)
                     /gene="hisG"
                     /locus_tag="VNU01_09775"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWH32945.1"
                     /translation="MSNEVRIAIPSKGRLREDCLKLLRQAGYPTSGFHGGGSIAEIQG
                     LSFIEMRPRDAAAAIEAGQLSAAFLATDIVMEHDLEALPALPLGFSRSTLVVASRDDD
                     GRSSVADLAGSVVATHLPELTRRFFGERGIDVRVVSMGGSLEGVCSAGLADAIVDLRE
                     TGTSLMMNRLRVLEEIRECQALFVRQAEVPALDDFTLRVEAVLSARRHRYVMLHLPQD
                     RVHELSTVFPGLASPTVLPLAERDDLVAVHFVVRSDELWSRLSDLRAMGATGIVALEP
                     QALLP"
     gene            complement(1718..2005)
                     /gene="hisE"
                     /locus_tag="VNU01_09780"
     CDS             complement(1718..2005)
                     /gene="hisE"
                     /locus_tag="VNU01_09780"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HWH32946.1"
                     /translation="MELAELEAVLRDRLATAPPESYSASLFRDPELAQRKIMEEAFEL
                     CLELGRAHAGQGDVRRTAEEAADLVFHVLAGLVGAGVAFDEVLKVLRERRR"
     gene            complement(2024..3289)
                     /gene="ahcY"
                     /locus_tag="VNU01_09785"
     CDS             complement(2024..3289)
                     /gene="ahcY"
                     /locus_tag="VNU01_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWH32947.1"
                     /translation="MSLPAHDVADLALAPKGKARIEWADRSMPVLRAIRERFAAERPL
                     EGQVVAACLHVTTETANLMRTLAAGGAQVTLVASNPLSTQDDTAAALVAEYGISTFAI
                     RAEDHDTYYKHIDAALDTQPTITMDDGCDLVNRLLSQRPEQAATVLAGTEETTTGVIR
                     LRAMEKAGALRYPIVAVNDTPTKHLFDNRFGTGQSTLDGIIRATNVLLAGKTVVVAGY
                     GYCGRGVASRAKGHGADVLVTEVDPIRALEAAMEGYRVAPMSQAAVEGDVFVTVTGNT
                     SVLRKEHFEAMKDGAIICNSGHFDVEIDIPALEKLSTARREVRQNAEEFQLADGRRIV
                     LLAEGRLVNLGAAEGHPAAVMDMSFADQSLAAEWLVRNIGSLETRVYDVPIELDAEVA
                     RLKLASMGKAIDTLTPEQEHYLNSYDIGT"
     gene            complement(3327..>4236)
                     /locus_tag="VNU01_09790"
     CDS             complement(3327..>4236)
                     /locus_tag="VNU01_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32948.1"
                     /translation="PPCTPSGSMPPPGFDELLRRAAAAPAGPLDVADLDRRVRRIRRN
                     RRIGTVAAVLVMAAPASLAGRAFLVPETSVDFASGGGRAIAPPTDEPSERSTVASTPT
                     PTPTATPSAPSVGGGVAVPGTSPSPAPSASPSGPRGQSSATVVISDPPGSPERRPDLP
                     GDARHPDVLLALVLDRVDVRQGERWKGWLEARNLGDEPIALSGAECWALWGLYRDGAW
                     VGGQADSVCGDIHPMRQLAPGASVRVPLDFDTVAGDDRDKGLRHLEPGEYQAAAALRV
                     QTREHGYSGVWYAETLTVTVSEADSE"
BASE COUNT          557 a         1556 c         1589 g          534 t
ORIGIN      
        1 tgcgcgtcct ggtgctcacg ctggccgccg gcggccagcg tgagcaccag gacgcgcacg
       61 gctcccgccg cggccagcgc cagcgcggtg gcgccggtcg tgcgcacgtc gtcgaccagc
      121 gccaccaccg gcgggacggc gccgcgcgcg acgaacgtgc cgaacggcgc ggtgcgccgc
      181 cgcgccggcg ccagccccgc ctggtcgggc ggggcgccgc ggcgggccag cagcgggcgg
      241 gcgcccgggc ccgccagcac gcgcggcagt tccacggcgc gcgagcgcgc gcgagccggc
      301 acggagggaa cccacgtgcg cacggcgggc agctcggccg ggccgacgcc gaaccgctcc
      361 cacaacagca cggccagcgt gggcgccagg ccgtgcccgc cgggcgtctt ggcccgcctg
      421 atggccgccg cgagcgcccc gccgtaggcg aagccggcca cggcgagcac ccccggcgcc
      481 ggccgtgacc agcccgcgtc gggcagcgcc agcgtggaga tctccgcccg gcacgcgggg
      541 cacagccaca ggccgtcgcc cagcacgggt gccaggcagc cgccgcagcg gcgcggtagc
      601 agcaggtcgc ccagggcgcg aagcatcggg ggacgctacc ggcccccggc tgccgacttt
      661 tttgccgtcc acagggttct ttggtgcaca gacgcaacct ttgcgccaat tcgcacgtct
      721 caagcgcgtg accgccctgg ccgaagggga cggttggagg cgatgagtat ggaaccccaa
      781 gtccagccga ggaccgcgat cctccgcccg gcgctgacgc tgcccgccat gctggcggcg
      841 ctggtcgccg tgatcgagct cgcgaaggcc ggcgcctagg gcaggagggc ctggggctcc
      901 agggcgacga tgccggtcgc gcccatcgcg cgcaggtcgc tcagccgtga ccacagctcg
      961 tcggagcgca ccacgaagtg cacggcgacg aggtcgtcgc gctcggccag cggcagcacc
     1021 gtgggcgagg ccaggccggg gaacaccgtg ctgagctcgt ggacgcggtc ctgcggcagg
     1081 tgcagcatca cgtagcggtg gcgccgcgcg gacagcaccg cctccacgcg cagggtgaag
     1141 tcgtcgagcg ccgggacctc cgcctggcgg acgaacagcg cctggcactc gcggatctcc
     1201 tccagcacgc gcagccggtt catcatcagg ctggtgccgg tctcgcgaag gtcgacgatc
     1261 gcgtcggcca ggcccgccga gcacacgccc tccagcgagc cgcccatcga caccacgcgc
     1321 acgtcgatgc cgcgctcgcc gaagaagcgc cgcgtcagct cgggcaggtg cgtggccacg
     1381 acgctgccgg cgaggtcggc caccgacgag cggccgtcgt cgtcgcggga ggcgaccacc
     1441 agcgtgctgc gcgagaagcc caggggcagc gcgggcagcg cctccaggtc gtgctccatg
     1501 acgatgtcgg tggccaggaa cgctgcggac agctgcccgg cctcgatcgc ggccgcggcg
     1561 tcgcgcgggc gcatctcgat gaacgacagc ccctggatct ccgcgatgga gccgccgccg
     1621 tggaagccgc tggtgggata gcccgcctgc cgcagcagct tcaggcagtc ctcgcgcagc
     1681 cggcccttgg acgggatggc gatgcggacc tcgttgctca tcgccgtcgc tcccgcagca
     1741 ccttcagcac ctcgtcgaac gccacgcccg cgcccaccag gccggcgagc acgtgaaaca
     1801 ccaggtcggc cgcctcctcg gcggtgcggc ggacgtcgcc ctgcccggcg tgcgcgcgcc
     1861 ccagctccag gcacagctcg aaggcctcct ccatgatctt gcgctgggca agctccgggt
     1921 cgcggaagag gctcgccgag taggactcgg gcggggcagt ggcgaggcgg tcccgaagga
     1981 ccgcctcgag ctcggcaagt tccatgcctg gcagcctatt gctctacgtg ccgatgtcgt
     2041 aggagttgag gtagtgctcc tgctccggcg tgagcgtgtc gatcgccttg cccatgctcg
     2101 caagcttcag gcgcgcgacc tccgcgtcca gctcgatcgg cacgtcgtac acgcgcgtct
     2161 ccaggctgcc gatgttgcgc accagccact ccgccgccag ggactggtcg gcgaagctca
     2221 tgtccatgac ggcggccggg tggccctcgg cggcgcccaa gttgaccagg cgcccctcgg
     2281 ccagcagcac gatgcggcgg ccgtcggcga gctggaactc ctcggcgttc tggcgcacct
     2341 cgcggcgcgc ggtggacagc ttctccagcg ccgggatgtc gatctcgacg tcgaagtggc
     2401 cggagttgca gatgatcgcg ccgtccttca tcgcttcgaa gtgctccttg cgcagcaccg
     2461 aggtgttgcc cgtgacggtg acgaacacgt cgccctcgac cgcggcctgg ctcatcggcg
     2521 cgacgcggta gccctccatc gccgcctcca gcgcgcggat ggggtcgacc tcggtcacca
     2581 gcacgtcggc gccgtgcccc ttggcgcgcg aggccacgcc gcggccgcag tagccgtagc
     2641 cggcgacgac cacggtcttg ccggccagca gcacgttggt ggcgcggatg atgccgtcca
     2701 gcgtggactg gccggtgccg aagcggttgt cgaacaggtg cttggtcggc gtgtcgttga
     2761 ccgcgacgat cgggtagcgc agcgcgccgg ccttctccat ggcgcgcagg cggatgaccc
     2821 cggtggtggt ctcctccgtg ccggccagca cggtggcggc ctgctccggg cgctgcgaca
     2881 gcagccggtt caccaggtcg cagccgtcgt ccatggtgat ggtcggctgg gtgtccagcg
     2941 ccgcgtcgat gtgcttgtag taggtgtcgt ggtcctcggc gcggatcgcg aacgtcgaga
     3001 tgccgtactc ggccaccagc gcggccgccg tgtcgtcctg cgtggacagc gggttggacg
     3061 cgaccagcgt cacctgcgca ccgccggccg ccagcgtgcg catgaggttg gcggtctcgg
     3121 tggtgacgtg caggcaggcc gcgacgacct ggccctccag cggccgctcc gcggcgaagc
     3181 gctcgcggat ggcgcgcagc acgggcatgg agcggtcggc ccactcgatg cgggccttgc
     3241 ccttgggggc gagtgcgaga tcggcgacgt cgtgcgcagg cagggacatg aaggctcttt
     3301 ctggtcggtg aggatcgcca gaaagcctac tccgagtcgg cctccgacac cgtcacggtg
     3361 agggtctcgg cgtaccacac gccgctgtag ccgtgctcgc gggtctgcac gcgcaacgcg
     3421 gcggccgcct ggtactcccc aggctccagg tggcgcagcc ccttgtcgcg gtcgtcgccc
     3481 gccacggtgt cgaagtccag cggcacgcgg acggaggcgc ccggggcgag ctggcgcatg
     3541 gggtggatgt cgccgcacac gctgtcggcc tgcccgccca cccaggcgcc gtcgcggtac
     3601 aggccccaca gggcccagca ctcggcgccg ctcagggcga tgggctcgtc gccgaggttg
     3661 cgcgcctcca gccagccctt ccagcgctcg ccctggcgca cgtcgacgcg gtccagcacc
     3721 agggccagca gcacgtcggg atgccgggcg tcgccgggca ggtccgggcg gcgctccggc
     3781 gagccgggcg ggtcggagat caccacggtc gccgacgact gcccccgcgg gccggacggt
     3841 gacgcggagg gcgcgggcga cggcgacgtg cccggcacgg cgacccctcc gcccacggac
     3901 ggcgcgctgg gcgtcgccgt cggcgtgggc gtgggcgtgg aggccacggt ggagcgctcg
     3961 ctgggctcgt cggtgggcgg cgcgatcgcg cggccgccgc cggaggcgaa gtccacggag
     4021 gtctccggca ccaagaacgc ccgccccgcc agcgacgccg gcgcggccat gacgagcacg
     4081 gcggccacgg tgccgatgcg ccggttgcgc cggatgcggc gcacgcggcg gtccaggtcg
     4141 gccacgtcca gcggccccgc cggggcggcc gcggcgcggc gcaacagctc gtcgaagccg
     4201 gggggcggca tgctgcccga gggggtgcac ggtggc
//
LOCUS       DATMKC010000331         3993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_294289, whole genome shotgun sequence.
ACCESSION   DATMKC010000331 DATMKC010000000
VERSION     DATMKC010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294289"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VNU01_09795"
     CDS             <1..1084
                     /locus_tag="VNU01_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWH32949.1"
                     /translation="LAHTEDLVLLLASNGAVRYASPGCDRIAPGLARPGASAFDPLDA
                     SDASRLRAALSDVVLEGAPRRIQVRGESPAGQRRTWEVRLDNRLRDPVLGAVVATFRD
                     VTGQMRTEHLAEGAAAVLRMVLAGAPRREALQTLVRMVEGAWPTVRATVLLADWDRGC
                     LRHGAAPSMPPHFVHAIDGLPIAEGAGACGTAAYRDAEVVVEDARRDPLLKDYRALMD
                     SLGARSLWSLPLHDGQGGVVGTLALYGTEVSSPTPEQWRLAQRLADLASLVIAGPRVA
                     SQSAASPAREPAMHAVARLTHRERAVLRLIALGHTNQEIADMLGLSVRTVETQRAAVS
                     GKSGAATRSQLVGLALEAGLLCAHHD"
     gene            1284..3227
                     /locus_tag="VNU01_09800"
     CDS             1284..3227
                     /locus_tag="VNU01_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32950.1"
                     /translation="MSLRAQIRAMFVLPLVAVLALQFAMLQYDRQADRHSSEIERLES
                     WRENIHQLYQVLLEAESAVRGYRLGGAVDQAHLVPYFAALRQAPQLVRELRPLAAGAP
                     QLTEHMRQLEELVAGRLSSLARLRRLPLELPPGNVIVTAQAEEGRRLTGLIREELEHF
                     IDEEARMEAVRRQGREAVAELGRVLQLAVPALLLLTFLLVRGTTSRLLRRLDLVGRNI
                     TDVVQGAPLVPLPEDSAESRRLNASMAEAEALLHRRERESTLSREEAERANAAKTAFL
                     SRMSHELRTPLTAVLGFSELLEMDDLTPQQRDSVRHIRTAGAHLLTLINEVLDISRIE
                     SGNLAVSIEPIELGPVVADALAIIRPMAEERGVRLPRASGRGMFVRADRQRLSQVLLN
                     LLSNAVKYNRDFGEIRLRCVAEGEVVRVAVTDTGLGIPPDMVPRVFNPFDRLGAERTG
                     VEGTGVGLVLSRGLVEAMGGSLTFVSVPGEGTTFTVRLPAAAEPSPLPQAPAPDVPEW
                     LPRSSPAGPTDGSRRVTVLYVEDNAANLDLVSRYLSQMPAVDLITTMQGRLAVDLARL
                     HRPDLILLDLHLPDLDGESVLRRLREDPHTATVPVAILTADATPGQERRLRAQGADHF
                     LHKPLDFARLAALLDPRGPVVQS"
     gene            3224..>3993
                     /locus_tag="VNU01_09805"
     CDS             3224..>3993
                     /locus_tag="VNU01_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH32951.1"
                     /translation="MTGWPADARILVVDDEPVNVHLLLRLLEQAGYQSVEGFADPAEL
                     AASVAEREPDLVVLDLHMPELDGFDLIRVMRQLLPADSFVPMLMLTADASARTREKAL
                     ELGASDFLTKPFDLTEVRLRVRNLLHTRGLHRRLAAQNRLLVDQVRDRTQDLHASRLE
                     LLQRLALAAEARDDDTYHHTVRVGRNAGRLAAALGFTPEEALLIEHAAPLHDIGKIGI
                     SDAILLKPGPLTPDEFAQIKTHAEVGARILGVRLDLCEL"
BASE COUNT          515 a         1433 c         1479 g          566 t
ORIGIN      
        1 gctggcgcac accgaggacc tcgtgctcct gctggcctcc aacggggcgg tgcgctacgc
       61 gagcccgggt tgtgatcgca tcgcgccagg gctggcgcgc cccggcgcca gcgccttcga
      121 tcccctggac gcctcggacg cctctcgctt gcgcgccgcc ctcagcgacg tggtgctgga
      181 gggcgcgccg cgccgcatcc aggtgcgtgg cgagtcgccg gcgggccagc ggcgaacgtg
      241 ggaggtgcgc ctggacaacc gtctgcgcga cccggtgctc ggggcggtcg tggcgacgtt
      301 ccgcgatgtg accgggcaga tgcgcaccga gcacctcgcg gagggcgccg cggcggtgct
      361 gcgcatggtg ctggccggcg cgccccggcg ggaggccctg cagacgctcg tgcggatggt
      421 ggagggcgcg tggcccacgg tgcgcgccac cgtcctgctg gcggactggg accgcggttg
      481 cctgcgccat ggtgcggcgc cgtccatgcc gccgcacttc gtgcacgcga tcgacggact
      541 gcccatcgcc gagggcgccg gcgcgtgcgg caccgccgcc taccgtgacg ccgaggtggt
      601 ggtggaggac gcgcgcaggg acccgctgct caaggactac cgcgcgctca tggacagctt
      661 gggggccagg agcctgtggt ccctgccgct gcacgacggc cagggcggcg tcgtgggcac
      721 gctggcgctg tacgggacgg aggtgtccag cccgacaccg gagcagtggc ggctcgcgca
      781 gcgcctggcc gatctggcca gtctggtgat cgccgggccg cgcgtcgcgt cacagtccgc
      841 tgcctccccg gcgcgcgagc ccgccatgca cgccgtcgcg cggctcacgc accgtgagcg
      901 tgccgttctg cgactgatcg ccctgggtca caccaaccag gagatcgccg acatgctcgg
      961 cttgagcgtg cgcaccgtgg agacacagcg cgccgcggtg tcgggcaagt cgggggctgc
     1021 cacgcggtca cagctcgtcg gcctggcgct ggaagcgggg ttgctgtgcg cgcaccacga
     1081 ctgagcgagt ccggcgtccc cggaccgggg gacgccaccc tgcgcagact gcggggaacc
     1141 cgctgcggaa agccgcggcg aactgcggat ggcgggtccg cgcagcggac ctacggtggg
     1201 ggcgagtccc gccgcggtgg ggcgggccgt ctcgaccgga gctcatgatg ctgcgcagca
     1261 ctgtcgctcg acgcgttggg cgcatgtccc tgcgcgccca gatccgggcg atgttcgtgc
     1321 tgccgctggt ggcggtgctc gccctccagt tcgccatgct gcagtacgac cgccaggccg
     1381 accgtcacag cagcgagatc gagcgactgg agtcctggcg ggagaacatc caccagctct
     1441 accaggtgct gctggaggcg gagagcgccg tgcgcggcta ccgcctgggt ggcgcggtcg
     1501 accaggccca cctggtcccg tacttcgccg cgctgcgtca ggcgccacag ctcgtccgcg
     1561 agcttcgccc gctggctgcg ggtgccccgc aacttacgga acacatgcgg cagctcgagg
     1621 agctggtggc gggccgtctg tccagcctgg ccaggttgcg ccggctgccg ctggagctgc
     1681 cgcccgggaa cgtgatcgtg acggcccagg cggaggaggg gcggcggctg acgggcttga
     1741 tccgcgagga gctggagcac ttcatcgacg aggaggcccg catggaggcc gtccgccggc
     1801 aaggccgtga ggcggtggcc gaactggggc gcgtgctgca gctggcggtg ccggcgctgc
     1861 tgctgctgac gttcctgctg gtccgtggca ccaccagccg cctgctgcgc cggctggacc
     1921 tggtgggccg caacatcacc gacgtggtgc agggcgcgcc gctggtcccg ctgccggagg
     1981 actccgccga gtcgcgccgc ctgaacgcgt cgatggccga ggccgaggcg ctgctgcacc
     2041 ggcgcgagcg cgagagcacc ctctcgcgcg aggaggcgga gcgcgcgaac gcggcgaaga
     2101 ccgcgttcct gtcgcgcatg tcccacgagc tgcgcacccc cctgaccgcg gtgctcggct
     2161 tctccgagct gctggagatg gacgacctca caccgcagca gcgcgacagc gtccgccaca
     2221 tccgcaccgc gggcgcgcac ctgctcacgc tcatcaacga ggtgttggac atctcccgga
     2281 tcgagtccgg gaatctcgcc gtgtcgatcg agccgatcga gctgggtccc gtggtggccg
     2341 acgcgctggc gatcatccgc ccgatggccg aggagcgggg cgtccgcctg ccacgcgcct
     2401 ccggccgcgg catgttcgtg cgcgccgacc ggcagcgctt gtcacaggtc ctgttgaacc
     2461 tgctgagcaa cgccgtcaag tacaaccgcg acttcggcga gatccgcctg cggtgcgtcg
     2521 ccgagggcga ggtcgtgcgc gtggccgtga cggacacggg cctgggcatc ccgccggaca
     2581 tggtgcccag ggtgttcaac ccgttcgacc gcctgggcgc ggagcgcacc ggcgtggagg
     2641 gcaccggcgt gggcctggtc ctgtcgcggg gcctcgtgga ggccatgggc ggttcgctca
     2701 cgttcgtgtc cgtccccggc gagggcacca cgttcaccgt gcgcctgccc gccgccgccg
     2761 agccatcgcc actcccgcag gcgcctgctc cggatgttcc cgagtggctc cccaggtctt
     2821 cgccggcggg cccgactgac ggctcgcgcc gggtgaccgt gctgtacgtg gaggacaacg
     2881 ccgccaacct ggacctggtg tcgcgctact tgagccagat gccggcggtg gacctgatca
     2941 ccacgatgca ggggcggctg gcggtggacc tggcgcggct gcaccggccc gacctgatcc
     3001 tgctggacct gcacttgccg gacctggacg gtgagtccgt gctgcgccgg ctgcgcgagg
     3061 acccccacac cgccacggtg ccggtggcca tcctcacggc ggacgccacc ccgggccagg
     3121 agcggcgtct gcgcgcccag ggcgccgacc actttctgca caagccgctg gacttcgccc
     3181 gcctcgcggc cctgctggat ccgcgaggcc cggtggtgca atcatgaccg gctggcctgc
     3241 ggacgcgcgc atcctcgtgg tggacgacga gccggtgaat gtccacttgc tcctgcgcct
     3301 gctggagcag gccggctacc agagtgtgga ggggttcgcc gatcccgccg agctggccgc
     3361 gtcggtcgcg gagcgcgagc ccgacctggt ggtactggac ctgcacatgc cggagctgga
     3421 cggcttcgac ctcatccgcg tgatgcgcca gctgctgccc gcggacagct tcgtgccgat
     3481 gctgatgctc accgccgacg cgagcgcgcg cacgcgcgag aaggcgctgg agctgggggc
     3541 gagcgacttc ctcaccaagc ccttcgacct gaccgaggtg cgcctgcgcg tgcgcaacct
     3601 gctgcacacc cgcggcctgc accgcaggct cgcggcgcag aaccgcctgc tggtcgacca
     3661 ggtgcgggac cggacgcagg acctgcacgc gtcgcgcctg gagttgctgc agcgcctggc
     3721 gctggccgcg gaggcccgcg acgacgacac ctatcaccac acggtgcgtg tcggccgcaa
     3781 cgccggccgg ctggcggccg ccctcggctt cacgccggag gaggcactgc tgatcgagca
     3841 cgccgcgccg ctgcacgaca tcggcaagat cgggatctcc gacgcgatcc tgctcaagcc
     3901 cggtccgctg accccggacg agttcgcaca gatcaagacg cacgccgagg tgggcgcgcg
     3961 catcctcggc gtgcgtcttg atctgtgcga act
//
LOCUS       DATMKC010000332         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_278033, whole genome shotgun sequence.
ACCESSION   DATMKC010000332 DATMKC010000000
VERSION     DATMKC010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278033"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VNU01_09810"
     CDS             <1..1030
                     /locus_tag="VNU01_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH32952.1"
                     /translation="AKPQRNRKGSIPVPAARPVTDERRIAFALVLLGALLLLREAGLW
                     AGDLLMFPLMLGAVGSGVIWATADETDRSRWTRLGLVAGPRWRRGPAAVARTAGGALL
                     VVLGMALFLATNGIFAALRVAGLATGVTLLGALLVLGPYLARLAQDLREERVARIRSQ
                     ERADVAAHLHDSVLHTLALIQRASATMSAGALARVQERELRAWLYGREQQGPGRLRGA
                     VDELAGRVERLHGVPVDAVVVGDAAMDEPLRALVAAAQEAAVNAARHSGAARVSLYVE
                     AEADGVTAWVRDEGRGFDPAAVPADRRGIADAVVGRVQRHGGTAVVASEPGEGTEVHL
                     WMPGATTA"
     gene            1027..1659
                     /locus_tag="VNU01_09815"
     CDS             1027..1659
                     /locus_tag="VNU01_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH32953.1"
                     /translation="MIRLFLVDDHELFLSGVRTELHGACDIVGTALDADTAAEAILAA
                     RPDVVLLDVHLPGGGGVEVLRQTRERLPGTRFLALSVSDSPDDVIGVIRAGARGYVTK
                     TITPAELVEAIQRIHDGDAVFSPRLAGFVLDAFAGELTGPLDPELDQLTPREREVLRL
                     IARGYAYKQVARRLEISIKTVESHVSAVLRKLQLSSRYELTRWAADRRLL"
     gene            complement(1625..1846)
                     /locus_tag="VNU01_09820"
     CDS             complement(1625..1846)
                     /locus_tag="VNU01_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32954.1"
                     /translation="MRRHDLDPVSLVFGLLFLAIAVALLVDAPALALLRLRWLLPTLG
                     IVVGVALLGSALRGDHETATADGDQPPSE"
     gene            complement(1846..>2599)
                     /locus_tag="VNU01_09825"
     CDS             complement(1846..>2599)
                     /locus_tag="VNU01_09825"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HWH32955.1"
                     /translation="LFTHSNHSRDPAPLAPPSLPTEPPSPRRPKSRLGRLTLALMFLA
                     LGVAGFLDATAVLSPSAGDYAALVLAAVGTGLVIGAWWGRARALIPLGVVLVPVVLVA
                     SILPEGPSLAGGSGEVYERPASSAALEQEYRLGAGQLTVDLRDVVLAPGEVRALRVTV
                     GFGEVTVVLPDSVTVVARADAGIGTVDLFGMRTGGLEPQLGGERTGSPFSGTLDLTAR
                     AGVGQVRVVRVAAGAPLHPFGDGLFGPGSEVF"
BASE COUNT          338 a          978 c          978 g          305 t
ORIGIN      
        1 cgcaaaaccc cagcgcaaca ggaaggggtc gatcccggtg ccggcggccc ggccggtcac
       61 cgacgagcgc cgcatcgcgt tcgcgctggt cctgctgggc gccctgctgc tgctgcgcga
      121 agcgggtctg tgggccggcg acctgctgat gttcccgctg atgctcggcg cggtcggctc
      181 cggcgtgatc tgggcgaccg ccgacgagac ggaccgcagc cgctggacgc gcttgggcct
      241 ggtcgcggga ccgcgctggc ggcgtggccc ggcggccgtc gcccgcaccg ccggcggcgc
      301 gctgctggtc gtgctgggca tggcgctgtt tctggccacg aacgggatct tcgcggccct
      361 gcgcgtcgcg gggctggcga ccggggtgac gctgctgggc gcgctgctcg tgctgggccc
      421 ctacctcgcc cggctggcgc aagacctgcg cgaggagcgc gtggccagga tccgctcgca
      481 ggagcgcgcc gacgtggcgg cccatctcca cgactccgtg ctgcacacgc tggcgctgat
      541 ccagcgcgcc agcgccacca tgtcggcggg ggcgctggcc cgggtccagg agcgcgaact
      601 gcgcgcctgg ctgtacgggc gcgagcagca ggggcccggc cggctgcgcg gggcggtcga
      661 cgagctggcc gggcgcgtgg aacggctgca tggcgtgccg gtggacgccg tcgtggtggg
      721 cgacgcggcg atggacgagc cgctgcgcgc cctggtcgcc gccgcccagg aagccgccgt
      781 caacgccgcg cgccactccg gcgccgcgcg cgtgtcgctg tacgtggagg cggaggccga
      841 cggcgtcacc gcgtgggtcc gcgacgaggg ccgcgggttc gacccggccg ccgtgccggc
      901 cgaccggcgc ggcatcgccg acgccgtggt gggccgcgtc cagcgccacg gcggcaccgc
      961 ggtcgtggcg agcgagccgg gcgaaggcac ggaggtgcac ctgtggatgc caggggccac
     1021 cacggcatga tccggctgtt cctggtcgac gaccacgagc tgttcctgtc cggcgtgcgc
     1081 acggagctgc acggcgcgtg cgacatcgtc ggcacggcgc tggacgccga cacggccgcg
     1141 gaggccatcc tggccgcgcg gcccgacgtc gtgctgctgg acgtgcacct gccgggcggg
     1201 ggcggcgtcg aggtgctgcg ccagacccgc gagcggctgc ccggcaccag gttcctggcc
     1261 ctgtcggtgt cggactcgcc ggacgacgtc atcggcgtca tccgcgccgg cgcccggggc
     1321 tacgtcacca agaccatcac cccggcggag ctggtcgagg cgatccagcg cattcacgac
     1381 ggcgacgcgg tgttctcccc ccggctggcc ggcttcgtcc tggacgcctt cgcgggggag
     1441 ctgaccggcc cgctcgatcc cgagctcgac cagctcaccc cgcgcgagcg cgaggtgctg
     1501 cgcctgatcg cgcgcggcta cgcctacaag caggtggccc ggcggctgga gatctcgatc
     1561 aagacggtcg agtcacacgt gtccgcggtg ctgcgcaagc tccagctttc cagccgctac
     1621 gagctcactc gctgggcggc tgatcgccgt ctgctgtagc ggtctcgtgg tcgccgcgca
     1681 gcgccgaacc cagcagggcg acacccacga cgatgccgag cgtcggcagc agccagcgca
     1741 ggcgcagcag ggccagcgcg ggcgcgtcga cgagcagcgc cacggcgatg gctaggaaca
     1801 gcagtccgaa taccagcgac accggatcga gatcgtgacg tcgcatcaga acacctccga
     1861 tccgggaccg aacaggccgt cgccgaacgg gtgcagcggc gcgccggcgg ccacgcgcac
     1921 cacgcgcacc tgcccgaccc cggcgcgcgc ggtcaggtcg agcgtgccgc tgaacgggct
     1981 gccggtgcgc tccccgccga gctgcggctc cagccctccg gtgcgcatgc cgaacaggtc
     2041 gacggtcccg atgcccgcgt ccgcccgcgc cacgaccgtg accgagtcgg gcagcaccac
     2101 cgtgacctcc ccgaacccga ccgtcacccg cagcgcgcgc acctcgcccg gcgccaggac
     2161 gacatcgcgc aggtccacgg tcaactgtcc cgcgccgagc cggtactcct gctccagcgc
     2221 ggccgacgac gcggggcgct cgtacacctc gccgctgccg ccggccagcg acgggccctc
     2281 cgggaggatg gacgccacca gcaccacggg caccagcacc acaccgagcg ggatcagcgc
     2341 ccgcgcccgg ccccaccagg cgccgatcac cagtccagtc cccaccgcgg ccaggaccag
     2401 ggcggcgtag tcccctgcgc tcggcgacag caccgccgtg gcgtcgagga agcccgcgac
     2461 gcccagcgcc aggaacatca gcgccagggt cagccggccc aggcgcgact tgggccggcg
     2521 cggcgaaggc ggttcggtgg gcagcgaggg cggcgccagg ggcgcggggt cgcggctgtg
     2581 attgctgtgg gtgaacagc
//
LOCUS       DATMKC010000333         4649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_36941, whole genome shotgun sequence.
ACCESSION   DATMKC010000333 DATMKC010000000
VERSION     DATMKC010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36941"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VNU01_09830"
     CDS             complement(<1..197)
                     /locus_tag="VNU01_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32956.1"
                     /translation="MRGDPVAGAGVEDHLDEDLAGLDKLDTPIEARVDAAARLWSAAW
                     PKLVAVALALAAWQAAAASGWR"
     gene            complement(187..945)
                     /locus_tag="VNU01_09835"
     CDS             complement(187..945)
                     /locus_tag="VNU01_09835"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH32957.1"
                     /translation="MSIVETQTGAAIAVRQVRKSYGPGHALALEGISLDVRPGEFICM
                     VGASGCGKTTLLNLIAGLDRPSDGTVAVAGKTGLLFQESALFPWLTVQANIELALRLI
                     GVPKARRAERARELIALVRLTGSEHRRPHQLSGGMRQRAALARALAQDADILLMDEPF
                     GALDAITRDLLHDELERVWADTGLTVLFVTHNVREAVRLGDRVLVLSSRPGRVVEEFA
                     VPLARPRRMDSAEVVTLAGTVTERLRQEVRRHAG"
     gene            1103..1912
                     /locus_tag="VNU01_09840"
     CDS             1103..1912
                     /locus_tag="VNU01_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWH32958.1"
                     /translation="MTPPTRLPSAPPAAHDGPPPAPADARPGYTVDQLAALTGVPSRT
                     IRHYQSEKVLPPPDKQGRIALYRELHVRRLELIAQLQDRGLSLKAIREALRDAEQGRL
                     SLEDWLGLSDQIRQPWSDDAPALLRHDELATRLAGRRPGLLAELAEAGLVKEQAGLPP
                     TYLVPSPGLLDVALRLEAAGVELRLAGGAAAVMRKHVRRAADDLVAHFLRHGAEGLGK
                     DAASTGEALDALRQLSAEAMRLIFAREVERALRERRDDLAAALSRARRDGS"
     gene            1921..2568
                     /locus_tag="VNU01_09845"
     CDS             1921..2568
                     /locus_tag="VNU01_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWH32959.1"
                     /translation="MAGHAPTSLGAGRPLLRGWSHLVAFAVMVVAGPLLVAGARPDAR
                     WFVAAHAASLTALFGVSALYHRKLWAPGPRAIMRRLDHATIFVLIAGTYTGVGGLILS
                     PGRRWLLALVWLGAAAGAAVQVAWPERRKLAAGTAIGLGWVAVLAAPLLLDALSAGRL
                     ALLLGGGVLYSAGAVAYATKRPRLAPAVFGYHEVFHLAVIGAAVAHTVVVAGLAA"
     gene            2565..3467
                     /locus_tag="VNU01_09850"
     CDS             2565..3467
                     /locus_tag="VNU01_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32960.1"
                     /translation="MTVQLHGLERRELDVHGRRLVCHVGGSGPALLLVHGITSSAATW
                     ARAARHLTAAHTVIAPDLPGHGESANPPGDYSLGAYAASLRDLLALLDVPRVTLVGHS
                     LGGGIALQTAYLFPELVERLALVSSGGLGSDVHLLLRAATLPGSELVLPLLTAPRVIG
                     AGDAVGRGLNRLGLRMPARVAEIWEGLRHLGAPEARRAFVHTARAVIGPAGQRVSAVD
                     RLYLAELLPTLVVWGAADRIIPAAHGHDAVTRMPGARLEVFERSGHFPHESEPRRFAR
                     LVSEFVATTDPAAAESLVATIRRK"
     gene            complement(3481..>4649)
                     /locus_tag="VNU01_09855"
     CDS             complement(3481..>4649)
                     /locus_tag="VNU01_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH32961.1"
                     /translation="SRLRPGYAGTVLEKRTATIVFDATDGGVWTVELAGGRGRARRGS
                     AADPTLVVHSSAATLARVVSGEASGVTAFLDGDLTVRGDLALSLQLDGLFAHQEERPV
                     HFARALETTAMGVRTAYLEAGPPDAPPVILLHGLGASKVSLLPVVAGLSAHHRVIALD
                     FPGFGKSSSPVGAPYDAAWFAKVVRRTMDAAEVDSAFLVGNSMGGRVSVELALQSPER
                     VRGIGLLCPAVAFDEYRLLRPFLQASRLDLALGVPGWKVPGPLPGWIVRNGLRAMFAD
                     HTRVPAANLDAAQQDFLRSIDNRRRRLAFLACTRRLGMESPARFWPRLVELQAPSLWV
                     FGDRDRLVSPNYAELVRSACPTAHVEVWDSCGHVPQFEHPHVTNQRLREFFAAC"
BASE COUNT          576 a         1789 c         1732 g          552 t
ORIGIN      
        1 cgccagcccg aggcggccgc cgcctgccag gccgccaggg ccagcgcgac cgccaccagc
       61 ttcggccacg ccgccgacca caggcgcgcc gccgcgtcga cacgcgcctc gatgggcgtg
      121 tccagcttgt ccaggccggc cagatcctcg tcgaggtggt cctccacccc cgcgccggcc
      181 accgggtcac cccgcatggc ggcgcacctc ctggcgcagc cgctcggtga cggtgccggc
      241 cagcgtgacc acctcggcgg agtccatcct gcggggccgc gccagcggca cggcgaactc
      301 ctccaccacc cggcccggac ggctggacag caccagcacg cggtcgccga ggcgcaccgc
      361 ctcgcgcacg ttgtgcgtga cgaacagcac cgtcaggccg gtgtccgccc agacgcgctc
      421 cagctcgtcg tgcagcaggt cgcgggtgat cgcgtccagg gcgccgaagg gctcgtccat
      481 cagcaggatg tcggcgtcct gggccagcgc ccgcgccagc gccgcgcgct ggcgcatgcc
      541 gccggagagc tggtgggggc ggcggtgctc cgagccggtc aggcgcacca gcgcgatgag
      601 ctcgcgggcg cgctccgccc ggcgagcctt cggcacgccg atcagccgca gcgccagctc
      661 gatgttggcc tgcacggtca gccacgggaa cagggcggac tcctggaaca gcaggccggt
      721 cttgccggca accgcgacgg tgccgtcgga gggccggtcg agcccggcga tgagattgag
      781 cagcgtggtc ttgccgcagc cggaggcgcc caccatgcag atgaactcgc cggggcggac
      841 atccagggag atgccctcca gggccagggc gtgcccgggc ccgtaggact tgcggacctg
      901 gcgcacggcg atggccgcgc ccgtctgggt ttccacgatg ctcatcgggc gaggattcgc
      961 ctgacggcgc gcccaccaca accaccagac cagggaaaac agtgcagggg ctgggatcgg
     1021 caccgtggaa acatggcaaa cgcgcctggt tttgacccgc tccacgttgc cagtgggcac
     1081 tgagacggtt acaactgtag acatgacacc tcccacacgc ctgcccagcg ccccgcccgc
     1141 ggcgcacgac ggcccgccac ccgcgccggc cgacgcccgg cccggctaca ccgtggacca
     1201 gctcgccgcg ctgaccggcg tacccagccg gaccatccgt cactaccagt cggagaaggt
     1261 gctgccgccg ccggacaagc agggccgcat cgcgctgtac cgggaactgc acgtgcgccg
     1321 cctggaactg atcgcccagt tgcaggaccg cggcctgtcg ctcaaggcga tccgtgaggc
     1381 gctgcgcgac gccgagcagg ggcgcctgtc gctggaggac tggctgggcc tgagcgacca
     1441 gatccgccag ccgtggtccg acgacgcgcc ggcgctgctg cgccacgacg agctggccac
     1501 gcgcctggcg ggcaggcggc ccgggctcct ggcggagctc gccgaggcgg gcctggtcaa
     1561 ggagcaggcc ggcctgccgc ccacctacct ggtgcccagc ccggggctgc tggacgtggc
     1621 cctgcgcctg gaggccgccg gcgtcgagct gcgcctggcc ggcggcgccg cggccgtgat
     1681 gcgcaagcac gtgcggcgcg ccgccgacga cctggtcgcc cacttcctgc gccacggcgc
     1741 cgaggggctc ggcaaggacg cggcgtccac cggcgaggcc ctggacgcgc tccggcaact
     1801 gtccgccgag gcgatgcgcc tgatcttcgc ccgcgaggtc gagcgcgccc tgcgcgagcg
     1861 ccgcgacgac ctggccgcgg cgctgtcgcg cgcccgccgg gacggctcgt gagcgccgcc
     1921 atggcagggc acgcacccac ctcgctcggc gccggccgcc cgctgctgcg cggctggtcg
     1981 cacctggtcg cgttcgccgt gatggtcgtg gccgggccgc tgctggtcgc cggcgcgcgc
     2041 ccggacgcgc gctggttcgt ggccgcgcac gcggccagcc tcaccgccct gttcggcgtc
     2101 agcgcgctgt accaccgcaa gctgtgggcg cccggcccgc gcgcgatcat gcgccggctg
     2161 gaccacgcca cgatcttcgt gctcatcgcc gggacctaca ccggcgtcgg cgggctgatc
     2221 ctgtcccccg gccgccgctg gctgctggcg ctggtgtggc tgggcgccgc cgccggcgcc
     2281 gcggtgcagg tggcgtggcc ggagcggcgc aagctcgccg ccggcaccgc gatcggcctg
     2341 ggctgggtgg ccgtgcttgc cgcgccgctg ctgctggacg ccctgtcggc gggccggctc
     2401 gcgctgctgc tgggcggcgg cgtgctgtac agcgccggcg cggtggccta cgccaccaag
     2461 cggccgcgcc tggccccggc ggtgttcggc taccacgagg tgttccacct ggcggtgatc
     2521 ggcgcggccg tcgcccacac cgtcgtcgtc gcggggctgg cggcatgacg gtgcagctgc
     2581 acggcttgga gcgccgggag ctcgacgtgc acggccgccg cctggtgtgc cacgtcggcg
     2641 gcagcggccc ggcgctgctg ctggtgcacg gcatcacgtc cagtgccgcg acctgggcgc
     2701 gcgccgcccg gcacctcacc gccgcccaca cggtgatcgc cccggacctg cccggccacg
     2761 gcgagtccgc gaacccgccc ggcgactact cgctgggcgc ctacgccgcg tcgctgcgcg
     2821 acctgctcgc gctcttggac gtgccgcgcg tcacgctggt gggccactcc ctgggcggcg
     2881 gcatcgcgct gcagaccgcc tacctgttcc cggagctggt ggagcgcctg gcgctggtgt
     2941 ccagcggcgg gctgggcagc gacgtgcacc tgctgctgcg cgccgcgacg ctgcccggct
     3001 ccgagctggt gctgcccctg ctgacggcgc cgcgcgtgat cggcgcgggc gacgcggtgg
     3061 gccgcggcct gaaccgcctg ggcctgcgca tgccggcgcg cgtcgccgag atctgggagg
     3121 ggctgcgcca cctgggcgcg ccggaggcgc ggcgcgcctt cgtgcacacc gcccgcgcgg
     3181 tgatcggccc cgcgggccag cgcgtcagcg ccgtcgaccg gctgtacctg gcggagctgc
     3241 tgcccacgct ggtggtgtgg ggcgcggccg accgcatcat ccccgccgcg cacggccacg
     3301 acgccgtcac tcgcatgccg ggcgcccgcc tggaggtgtt cgagcgctcc gggcacttcc
     3361 cgcacgagag cgagccgcgc cgcttcgccc gcctggtcag cgagttcgtc gccaccacgg
     3421 accccgccgc tgcggagtca cttgtggcca ctatccgccg caagtgactc cgcagcgtgg
     3481 ctagcaggcg gcgaagaact cgcgcaggcg ctggttggtg acgtgcgggt gctcgaactg
     3541 cggcacgtgg ccgcaggagt cccacacctc cacgtgcgcc gtcgggcacg ccgagcgcac
     3601 cagctccgcg tagttcggcg acaccagccg gtcgcggtcg ccgaagaccc agaggctggg
     3661 cgcctgcagc tccaccaggc gcggccagaa gcgcgccggg gactccatgc ccagccgccg
     3721 cgtgcacgcc aggaacgcca ggcgccggcg gcggttgtcg atggagcgca ggaagtcctg
     3781 ctgcgccgcg tccaggttcg cggccggcac gcgcgtgtgg tcggcgaaca tcgcccgcag
     3841 gccgttgcgc acgatccagc cgggcagcgg cccgggcacc ttccagccgg gcacccccag
     3901 cgcgaggtcc aggcgcgagg cctgcaggaa cggccgcagc aggcggtact cgtcgaacgc
     3961 gaccgccggg cacaaaaggc cgatgccgcg cacgcgctcc ggcgactgca gcgccagctc
     4021 caccgacacg cgcccgccca tcgagttgcc gaccaggaac gccgagtcga cctcggcggc
     4081 gtccatggtc cgccgcacga ccttggcgaa ccaggcggcg tcgtacggcg cgccgaccgg
     4141 cgaggaggac ttgccgaagc cggggaagtc cagcgcgatg acccggtggt gcgccgacag
     4201 ccccgccacc accggcagca gcgacacctt cgacgcgccc aggccgtgca gcaggatcac
     4261 cggcggcgcg tccggcgggc cggcctcgag gtaggcggtg cgcacgccca tggcggtcgt
     4321 ctcgagcgcc cgggcgaagt ggacgggccg ctcctcctgg tgcgcgaaca ggccgtcgag
     4381 ctggaggctg agcgcgaggt cgccgcggac ggtgaggtcg ccgtcgagga aggcggtgac
     4441 gccggaggcc tcaccggaga cgacgcgggc gagggtggcg gccgagctgt gcacgaccag
     4501 cgtcgggtcg gccgccgacc cgcggcgggc gcggccgcgg ccgccggcga gctcgacggt
     4561 ccacacgccg ccgtcggtcg cgtcgaagac gatcgtggcg gtccgcttct cgagcaccgt
     4621 ccccgcgtag cccggccgca gcctcgagg
//
LOCUS       DATMKC010000334        16566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_205967, whole genome shotgun sequence.
ACCESSION   DATMKC010000334 DATMKC010000000
VERSION     DATMKC010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 16566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16566
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205967"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1516)
                     /gene="arsJ"
                     /locus_tag="VNU01_09860"
     CDS             complement(248..1516)
                     /gene="arsJ"
                     /locus_tag="VNU01_09860"
                     /inference="COORDINATES: protein motif:HMM:NF033734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HWH32962.1"
                     /translation="MSTATDTVARGDLRNYALVTGAYWADTIADGAIRTLVLFYFYEL
                     GYSPFAVASLFLFYEVFGVVTNLVGGFLAARLGLKSTLVMGLGTQIVAVGMLAVVPEA
                     WLVVPYVMVAQALSGIAKDLTKMSSKSAVKLVVPKDRPGALYRWVAVLTGSKNALKGV
                     GFFLGGLLLSVVGFRAGLGVLAVLVATALVVVLAMMRGDLSRPDATAKFRQMFSNNRA
                     VNVLAGARVFLFASRDVWFVVGLPVYLRTVLGWSFWQVGTFMAVWVIGYGLVQAAAPR
                     LMGRRRGGGPRPDPDGRTATWLAFALAAFPAGIALTLATDVDPGVVIVAGLIVFGFIF
                     AMNSAVHSYLILAYAEGDRVAMNVGFYYMANAGGRLAGTVLSGALYQWQGLEACLWAS
                     VAFVLAAGLLSRLLPVRPGAGAMPAPARPA"
     gene            complement(1513..2550)
                     /locus_tag="VNU01_09865"
     CDS             complement(1513..2550)
                     /locus_tag="VNU01_09865"
                     /inference="COORDINATES: protein motif:HMM:NF033735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HWH32963.1"
                     /translation="MTRIGINGFGRMGRLGLRAGWGREDLQFVHVNEVKGDAATAAHL
                     LTFDSVHGRWHREARGEGGALHVEGVELGVTRHAAPGDVPWDDLGVEVVLECSGNFRT
                     IDALAPYFARGVRKVVVAAPVNDDRALNVVMGVNHTSYDPERFDVVTAASCTTNCLAP
                     VVKVLHEGIGIRHGQITTLHDMTNTQVIVDAPHKDLRRARAASMSLIPTTTGSATAIG
                     RIFPELQGRLDGLAVRVPLLTGSLTDCVFEVERATDAEEVNGLLRAAAAGPLEGILGY
                     EERPLVSTDYRTDGRSAIVDAPCTRVVGGTQVKVLAWYDNEWGYANRMVELAAMVGAT
                     LPAAAQRAPVG"
     gene            complement(2555..3700)
                     /gene="arsB"
                     /locus_tag="VNU01_09870"
     CDS             complement(2555..3700)
                     /gene="arsB"
                     /locus_tag="VNU01_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWH32964.1"
                     /translation="MTAAAPQGEAAVMARLSTLDRFLPLWIGLAMAGGLALGGLVPGI
                     DAALDRLAVGTVSLPIALGLLLMMYPVLARVRYEELGTLGGGRRLLAASLFLNWVVGP
                     LLMFALAWIFLADHPEFRTGLIIVGLARCIAMVLIWSDLACADNHATALMVAVNSLFQ
                     IVAYSLLGWLYLTGLPSLLGLDTQGFEVSLWEVARTVLIFLGIPLAAGIVTRRVGIRR
                     RGRAWYEQAFLPRIGPLALYGLLFTIVMLFALQGEAITSEPLDVVRIAVPLLVYFLAM
                     FGLSFWLGYGLGFGYARTASLAFTAASNNFELAIAVAVGVFGATSGQALAGVVGPLIE
                     VPVLVGLVYVALWAGRRLFGPDAVRRNPIGAVRPSETVSDPVPESIR"
     gene            complement(3789..4679)
                     /gene="arsM"
                     /locus_tag="VNU01_09875"
     CDS             complement(3789..4679)
                     /gene="arsM"
                     /locus_tag="VNU01_09875"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWH32965.1"
                     /translation="MADLTSACCATEAQASCCGPADKDACCDPAAHAEGGCGCAAGAG
                     EGADIREAVRQRYAAAARAAGAGEGCCGAAGVAVTDQAGTQVFGDALYAPGETEGAPA
                     DAVAASLGCGVPTAVADLHAGETVLDLGSGAGADVLISARRVGPTGRAYGLDMTDEML
                     ALARANAEAAGAENVTFLKGYIEDIPLPDGSVDVVISNCVVNLAGDKQRVFAEVARVL
                     RPGGRFAVSDVVADAGMDDATRADMAAWTGCIAGALTREEFAEGLAAAGLTDVEIAET
                     HRVHTHAAAAIVRATKAAHG"
     gene            complement(4667..5011)
                     /locus_tag="VNU01_09880"
     CDS             complement(4667..5011)
                     /locus_tag="VNU01_09880"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWH32966.1"
                     /translation="MTTRPTALPAGPSADACCAPSTGLDASLDAERLAELAKALSEPV
                     RVSILDVLRRHGEPLCQCELVPLFNIPQSTLSHHVRKLADAGLITVERRHRWAYYSVR
                     PEALEELTTWLT"
     gene            5455..8547
                     /locus_tag="VNU01_09885"
     CDS             5455..8547
                     /locus_tag="VNU01_09885"
                     /inference="COORDINATES: protein motif:HMM:NF023335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HWH32967.1"
                     /translation="MTPLRPGLYEVLITEGLRAHMDALADASSIRDRPLEPSEAPDRI
                     AWHIGREVERALADVSEDRRVDVAVRVARALLERLGAMAPADGDALPAEPGAVLRAIA
                     GRRPDGSPDLPAEPLTPLLDTTLLTNAPGEPTLVTQLRSEIASADALDVVMAFIRRTG
                     IAPLLDALRRHVERGRPLRVLTTTYTGSTEARALDELRRIGADVRISYDVGTTRLHAK
                     AWVFHRSGGVSTAYVGSSNLTHSAQVTGLEWNVRISEARNPDVVAKFAAVFESYWASG
                     EFVPYDRDRFAQEHARAGRADSGPHVMLSPLEVRPLPFQERLLELLQVSRRRGHHRNL
                     LVAATGTGKTVMAALDYAGLRARMPRARLLVIAHREEILDQSLATFRHVLRAPSFGEK
                     WVGGARPTRFEHVFASIQSLNAADLDNLPSDHFDVVIVDEFHHAAATSYRRVLDHLAP
                     VELLGLTATPERSDGLPVLHWFEDRIAAELRLWDAIDQQHLVPFTYFGVHDNLDLRRV
                     PWRRGRGYDLDALTNVYTSDDAWARLVVAQVHEHVDAAGMRALGFCVSIDHARFMARH
                     FTRHGIPAVAIWGDSAREQREQALRDLAAGRVRCVFSVDLFNEGIDVPAVDTVLMLRP
                     TESPVLFLQQLGRGLRRSDGKSVCTVLDFVGQHRHEFRFDRRLRALLGGTRTDVERQV
                     KEGFPYLPAGCHMYLDPVAADIVLRSLREAIPSRWAAKVDELRSLRRDLGYVDLARFL
                     AVSGLDLDDVYEAQRGWSDLQQAAGVSVAAAGPHERPLRRGVGRLLHVDDEERLGTYR
                     RLLAGDRAPQAERLGERERRLARMLVAVVTDTALDRQTTLQQALDLLWRHPQALADLR
                     DLVAVLRERIDHAHTPLAGHPHVPLQVHARYSRLEILAAMGVGHGAKVAPWQTGVYEA
                     RSARADLLAFTLDKSGRRFSPTTRYRDYAISRTLIHWESQSVTRADSTTGRRYRGHAE
                     LGWSILLFTRLNVNERAFWFLGPATYRTHQGERPMAITWELAHPLPGDLYARFAAAVA
                     "
     gene            complement(9096..12233)
                     /locus_tag="VNU01_09890"
     CDS             complement(9096..12233)
                     /locus_tag="VNU01_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWH32968.1"
                     /translation="MAVMPPTPEQARVLEASEPFVPVAAGAGAGKTTLLVERIWRDVE
                     HEGVPIERILVVTFNRSAAAHLRDRLQQRFGDRDAGRSSDRASLDLSAGWIGTFHGLS
                     ARIVRMHPFLAGVDPEFSELDDVEADALVEQALDDALAATTHPGLLDALAQAVYDGGI
                     RQAVLSAYARLRAAGHREPRLVLPPPPVLDPADAVGAARLLDELVVHPDLHERHAAGI
                     AAGRVLLSGEGPLPKCPKIHGTAKKGVKPLIDDANELLCRIWTDRVALFARPQLEALA
                     EVMERFATGYAQRKVELGALDYEDLQFHALDVLRAGVSLGLDRVYVDEFQDANAIQNE
                     IVDALGARRTWVVGDGGQAIYGFRHATSRFFAERVGDPPRYALRDNHRSQPELMEALN
                     DVFAHALAGEPAFRSLHATAATTPAPPLRRPPIELVSVSDADRERSTRDQEAEAVADA
                     VEGLLHDGYANRDVAVLFRALTMVEPYERALRVRGIPTYLVAGRGFFSHQQVADVLAL
                     LHLVDNPHHEDHLVRVLGSPYVNASDGDLVALRRHAGTKRPLWPAVPGIPALAPLVDL
                     VAALRPVLRESGLPALVEAAIAANGYDLAVLGLEGGRRRFANLRRLVQMAESFAEVRG
                     PDLPGFLHALAERDRLGQDPGEAVVVDPDLDAVRLTTIHAVKGQEFPAVVIADGSHGM
                     PGQQDLLMVDTDGRAGFRAKLAGGDPVDLFGAADLKERLQAEAAAEERRVFYVALTRA
                     KRHVTVVGRPDGRGGPNCMWSLLKQAIDAGSAFAAETAVAAASIVVPERRPAPAAPPV
                     AAAHAPVPAEPLSVDALAGRRLSFTALQALHEAPGEGAHSGAVPAADGGGAVVGTLVH
                     VALERHRWLGPHSATAPPPGWAAPLAASLGLGPLPPAHAERAESLVRDVLASPLAERI
                     RTALRVETEHPFAVVVDGVLLAGAIDLLAWLPDGTLLVVDWKTHALDGKQPNGLRGEY
                     ELQAALYSLAALRAGTVRVDRAWVFLGLSESPRTTMVVDADVPTIETVVRSALSGASA
                     RSTLRRTPPLRANRR"
     gene            complement(12224..15016)
                     /locus_tag="VNU01_09895"
     CDS             complement(12224..15016)
                     /locus_tag="VNU01_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWH32969.1"
                     /translation="MPLTLHRAATFAHAWDVAHELWTATGNGFLVAPDRHAVTRELIR
                     AVGPSGVLFDREAVTWAALRDRVLRVAEHPPRAVPSRVAVRLALQDAIRRTPLERLGA
                     SARGPGFLTALERALAEIREAGLAATELATRAPDPVSAEIAAVLAAAADAAHPADVRW
                     SAVRQADALTEFRPVVACGIDDLAPSAWSLLRALARVTEVHLVMPYDDARRGHEARHA
                     RQAGWGAQADQVTTHRAGGGGRIEDVVFEDRPPVADGRAVHRIGAAGTRGMHRAALSA
                     LLQAIRGGVRPAQCALVVPGLSRARDDLDRLLADWEVPARRLTRRRVLETRLGLALVH
                     LLRFSELDRDASGALAALLGWLRSPYSGADPGEVDAFERDARHAGLDQRRELIGRWGE
                     VAVPARRLVAAARDGVRAQLAALVAVGEGALAALPEAPPTHADELDRRALAALAGLAA
                     DLHDVPLEDDALPRARGPLPSGELGALIADLTVPDERGPEAGLAVLDYASLRGRVFHT
                     VALCGLDGDGLPSGPAPDALLGAFRAVLPERLVPRAPGTSEARLRFVHALAAATDQVI
                     LVRRVADDEGRPVAPSPFWMETCRAAGLDADGLDVRPDPDGEILARDEAGPEREMLRR
                     LALDGLAVPGLLANAAARRVRPLGLHPGALDHVTEISVTGVETFLGCPYGWLHGRYLS
                     PDPLVRPFDPAAEGTIGHEIMAAVYARVAAGPDPGPCDLARLPAYRAALADCLREVLV
                     RERPNRSTTAFDAFGTRLAVHLELLLANEAASGPRMRPVAVEHALRDEALLDVHPPLA
                     LTGRLDRLDADHRHAVVVDYKRSGRDYRGTEELPKRLQPPLYGYLAAAERGLESAGGL
                     YVSLLGGRRDGLVRSDADYAERPPRSVDAGEWRGMVDEAIDAARQVAADLRGGRLAGP
                     PSAGCARWCGCGALWR"
     gene            complement(15164..16291)
                     /locus_tag="VNU01_09900"
     CDS             complement(15164..16291)
                     /locus_tag="VNU01_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32970.1"
                     /translation="MRDPAVTIRHRVEQHGGRLAVSVREIRDTFGLSRLTLAGRQRII
                     TALVDAGLTVDPAFTTAALDDLILLTSAHRVARVASAPPLPTEPRPPRTPAGPAGEAI
                     QPPNGKRPTKRFLVIAALAVVALVVVVATLSAPRDETSASTAPGTTPRTIAAATRPAP
                     AQRARLLLARAERAAARDRLDVAEGYLARVPAEIEAQDPVVAQEIARARVIVDRTRRY
                     LAGERSGRGGRYARAARTMKALGGFRDAPQKARAYSRKHALVLVSQARRDLENDRPGQ
                     ALAKLGRAEGWSPLPAISVLRRQVRERQAQLARPTPPEPTTASSSECDPSYQGACLDP
                     SSPDYDCAGGSGDGPDYTGPVQVVGPDRFGLDRDGDGYACE"
BASE COUNT         2364 a         6144 c         5958 g         2100 t
ORIGIN      
        1 gtcacaggtg caggacctca cgacctcgca ggccgttggt gctcatcggc ggttcctctc
       61 gtggcgtggc gccgtgtgtg tgaccgccgg tcggatcccc ggcggggacc cggcgtggca
      121 gccgcggtcc cgtgcccgcg cgcctgcgta cggaaacggc gcgggccgcc cggacgtccg
      181 caggcacccg cacggcgacg gccgccgggg gccgccccgc cggagccgcc cggcgggcac
      241 ggcgccgcta cgcggggcgc gccggggccg gcatcgcccc ggcccccggg cggacgggta
      301 gcaggcgcga cagcaggccc gccgccagca cgaacgccac cgaggcccac aggcaggcct
      361 ccaggccctg ccactggtac agagcccccg acaggaccgt cccggccagc cgcccgccgg
      421 cgttggccat gtagtagaag ccgacgttca tggccacccg gtcgccctcg gcgtaggcga
      481 ggatcagata cgagtggacg gcggagttca tcgcgaagat gaacccgaac acgatcaggc
      541 ccgccacgat caccacgccc gggtcgacgt cggtcgccag ggtcagggcg atgccggccg
      601 ggaaggccgc cagggcgaag gccagccagg tggcggtgcg cccgtcgggg tcgggccggg
      661 gacctccgcc ccgccggcgg cccatcaggc gcggggcggc ggcctgcacc aggccgtagc
      721 cgatcaccca cacggccatg aaggtgccca cctgccagaa gctccagccg agcacggtgc
      781 gcaggtagac gggcaggccg accacgaacc acacgtcgcg cgacgcgaac aggaacacgc
      841 gtgcgccggc cagcacgttg accgcccggt tgttcgagaa catctgacgg aacttcgccg
      901 tcgcgtcggg tcgcgacagg tcgccgcgca tcatggccag caccaccacg agcgccgtcg
      961 ccaccagcac ggccaggaca cccagcccgg cccggaatcc cacgacgctc agcagcaggc
     1021 cgcccaggaa gaagcccacg cccttcaggg cgttcttgga cccggtcaga acggccaccc
     1081 accggtacag cgcccccggc cggtccttcg ggaccaccag cttcacggcg ctcttggagc
     1141 tcatcttggt gaggtccttg gcgatccccg acagcgcctg ggcgaccatc acgtagggga
     1201 cgaccagcca cgcctcgggg accacggcga gcatgcccac ggcgacgatc tgcgtgccga
     1261 ggcccatcac cagcgtcgac ttcaggccga gccgcgccgc gaggaagccg ccgacgaggt
     1321 tggtgaccac gccgaagacc tcgtagaaca ggaacagcga ggccaccgcg aacgggctgt
     1381 agcccagctc gtagaagtag aacagcacca gcgtgcggat cgcgccatcg gcaatcgtgt
     1441 ccgcccagta ggcccccgtg acgagcgcgt agttgcgcag gtcgccccgc gccacggtgt
     1501 ccgtcgccgt gctcacccca ccggtgcccg ctgcgccgcg gcgggcaggg tcgctcccac
     1561 catggccgcc agctcgacca tccggttcgc gtagccccac tcgttgtcgt accaggccag
     1621 caccttgacc tgggtgccgc ccacgacgcg ggtgcagggc gcgtcgacga tggccgagcg
     1681 cccatcggtg cggtagtccg tggacaccag cgggcgctcc tcgtagccga ggatcccctc
     1741 gagcgggccg gcggcggccg cgcgcaggag cccgttgacc tcctcggcgt ccgtcgcccg
     1801 ctccacctcg aacacgcagt cggtgagcga gccggtcagc agcggcacgc gtaccgccag
     1861 gccgtccagc cggccctgca gctcggggaa gatccgcccg atcgccgtgg ccgatcccgt
     1921 ggtggtgggg atcagcgaca tgctcgccgc ccgcgcgcgg cgcaggtcct tgtggggcgc
     1981 gtccacgatc acctgcgtgt tggtcatgtc gtgcagcgtg gtgatctggc cgtggcgtat
     2041 gccgatgccc tcgtggagca ccttcaccac cggcgccagg cagttggtgg tgcacgaggc
     2101 ggcggtgacc acgtcgaacc gctccgggtc gtagctcgtg tggttgacgc ccatcaccac
     2161 gttcagcgcc cggtcgtcgt tcaccggggc ggcgaccacg accttccgga ccccgcgggc
     2221 gaagtagggc gccagcgcgt cgatggtgcg gaaattgccc gagcactcca gcaccacctc
     2281 gacgccgagg tcgtcccacg gcacgtcgcc gggcgcggcg tgccgggtca cgcccagctc
     2341 cacgccctcc acgtgcagcg ccccgccctc cccccgggcc tcgcggtgcc accggccatg
     2401 gaccgagtcg aaggtgagca ggtgggccgc cgtggcggcg tcgcccttca cctcgttgac
     2461 gtgcacgaac tgcaggtcct cacggcccca cccggcgcgc aggcccagtc gtcccatgcg
     2521 gccgaagccg ttgatgccga tgcgtgtcat ggcctcacct gatcgattcg gggacgggat
     2581 cgctgacggt ctcggacggc cgcaccgccc cgatggggtt gcgccgcacg gcgtccggcc
     2641 cgaacaggcg gcggccggcc cacagcgcca cgtacaccag gccgacgagg accggcacct
     2701 cgatcagcgg gcccaccacc ccggccagcg cctgaccgga ggtcgcaccg aacaccccca
     2761 ccgccacggc gatcgccagc tcgaagttgt tcgaggcggc ggtgaacgcc agggatgccg
     2821 tgcgggcgta gccgaagccc aggccgtacc cgagccagaa cgacagcccg aacatggcca
     2881 ggaagtagac gagcagcggc acggcgatgc gcaccacgtc gaggggctcc gaggtgatcg
     2941 cctcgccctg cagcgcgaac agcatcacga tcgtgaacag cagcccgtag agggccagcg
     3001 ggccgatgcg cggcaggaac gcctgctcgt accacgcccg cccgcgccgc cggatgccga
     3061 cacggcgcgt gacgattccc gccgccagcg ggatgccgag gaagatcagc acggtccgcg
     3121 ccacctccca cagcgagacc tcgaacccct gggtgtcgag gcccagcagg ctcggcaggc
     3181 cggtcaggta caaccacccc agcagtgagt aggcgacgat ctggaacagc gagttcaccg
     3241 cgaccatcag cgccgtggcg tggttgtcgg cgcacgccag gtcggaccag atgagcacca
     3301 tcgcgatgca ccgggccagg cccacgatga tcagcccggt gcggaactcg gggtggtcgg
     3361 ccaggaagat ccaggccagc gcgaacatca gcagcggccc gacgacccag ttcaggaaca
     3421 gcgacgccgc cagcagccgg cgcccgccgc ccagcgtgcc gagctcctcg tagcgcaccc
     3481 gcgccagcac cgggtacatc atcagcagca ggccgagcgc gatcggcagc gagacggtgc
     3541 cgacggccag gcggtcgagc gccgcgtcga tccccgggac gaggcctccc agcgccagcc
     3601 ccccggccat cgccaaaccg atccacagcg gcaggaagcg gtccagcgtg gacagccgcg
     3661 ccatcaccgc cgcctcaccc tgcggtgcgg ccgccgtcaa cggcgtgccg ccatgcgccg
     3721 cgcggagagc gccggagcgc cagcgtcgtg cacccgcacg cccacgccgg cgcccgcggc
     3781 gccacccctc atccgtgcgc ggccttcgtg gcgcgcacga tggcggcggc cgcgtgcgtg
     3841 tgcacccggt gcgtctccgc gatctccacg tcggtgaggc cggccgcggc caggccctcg
     3901 gcgaactcct cccgggtcag cgcccccgcg atgcagccgg tccacgcggc catgtcggcg
     3961 cgggtggcgt cgtccatccc ggcgtcggcc accacgtccg agacggcgaa acggccgccg
     4021 ggccgcagca cccgcgccac ctcggcgaac acgcgctgct tgtcgccggc caggttgacg
     4081 acgcagttcg agatcaccac gtccaccgag ccgtccggca gcgggatgtc ctcgatgtag
     4141 cccttgagga acgtgacgtt ctccgcgccc gccgcctccg cgtttgcccg cgccagcgcg
     4201 agcatctcgt cggtcatgtc caggccgtac gcccgccccg tcgggcccac gcgccgcgcc
     4261 gagatcagga cgtcggcgcc ggcgcccgag ccgaggtcga gcaccgtctc cccggcgtgc
     4321 agatcggcca ccgcggtggg cacgccgcag cccagcgagg ccgccacggc gtccgccggc
     4381 gcgccctcgg tctccccggg ggcgtacagg gcgtcgccga acacctgcgt gcccgcctgg
     4441 tcggtcaccg ccacgcccgc cgcgccgcag cacccctccc ccgccccggc ggcacgggcg
     4501 gcggccgcgt agcgctgccg caccgcctcg cggatgtcgg cgccctcccc cgctccggcc
     4561 gcgcagccgc acccgccctc ggcgtgcgcg gccgggtcgc agcacgcgtc cttgtccgcc
     4621 ggcccgcagc aggacgcctg cgcctcggtc gcgcagcagg cggaggtcag gtcagccatg
     4681 tcgtcagctc ctcgagcgcc tcggggcgca cggagtagta ggcccaccgg tgacgccgct
     4741 ccaccgtgat caggcccgcg tcggccagct tgcgcacgtg atgcgagagc gtcgactgcg
     4801 ggatgttgaa cagcggcacc agctcgcact ggcacagcgg ctccccgtgc cggcgaagga
     4861 cgtcgaggat gctcacccgc acgggctccg acagcgcctt ggccagttcg gccaggcgtt
     4921 cggcgtcgag gctcgcgtcg agccccgtgg agggcgcgca gcaggcgtcc gcggaggggc
     4981 cggcgggaag tgcggtgggg cgagtggtca tatcggtgat cgtcgttgtg acgctgctca
     5041 cggtacctga tccgcgcagg tcataccgac gttcaccgtt tcaaggcggt gaaagtttag
     5101 ggtcgcggcg tcggccgcaa gtcccccgcc cccagctgcg ggcaccatcg cgcgaagttc
     5161 gaccttcccg ctgtggaacg acgggggcca cggcgaggtg cacacggacg agggagccct
     5221 gaaagtcccg aggggtcgcg gaagcggtga caaccaggat gacgcgctcc gccacacgcc
     5281 cagcagcacg ctcagggcga tgccgtcggc ctacagctcg tagcgcgttc gcgccgaatg
     5341 gcggaccgcg tttgagccgg cgtccagtac gtgggacggg cgcatcgcgg ctgcgggtcg
     5401 tgcgccgccc ccgttccgcc gaccgtggcg gagaccgcgg gttagggtgc cgtcgtgacg
     5461 ccgctgcgcc cggggctcta cgaggtgctg atcaccgagg ggctgcgggc acacatggat
     5521 gcgctcgccg atgcgtcgtc gatccgggac cgcccgctgg agccctccga agcgcccgac
     5581 cggatcgcct ggcacatcgg ccgcgaggtg gagcgggcac tcgcggacgt gtcggaggac
     5641 aggcgggtcg acgtggctgt gcgcgtggcg cgggcgctgc tggagcgcct gggcgccatg
     5701 gccccggccg acggggacgc actgcccgcg gagcccgggg cggtgctccg cgccatcgcc
     5761 ggacgccggc cggacggctc ccccgatctg cccgccgagc ccctcacacc ccttctggat
     5821 accacgctcc tgaccaacgc tcccggcgag ccgaccctcg tgacgcagtt gcggtcggag
     5881 atcgcctcgg ccgacgcgct cgacgtggtg atggcgttca tccgccgcac gggcatcgca
     5941 cccctgctcg atgcgctgcg gcgccacgtg gagcgcggtc gcccgctgcg ggtgctgacg
     6001 accacctaca ccggctccac ggaggcccgg gcgctggatg agctccggcg gatcggtgcc
     6061 gacgtgcgca tctcctacga cgtcggcacg acccggctgc acgccaaggc gtgggtcttt
     6121 caccgctccg gcggcgtctc caccgcgtac gtcggctcgt cgaacctgac gcactcggcg
     6181 caggtcaccg ggctcgagtg gaacgtgcgc atctcggagg cgcgcaaccc cgatgtggtc
     6241 gccaagttcg cggcggtctt cgagagctac tgggccagcg gcgagttcgt gccgtacgac
     6301 cgcgaccgct tcgcgcagga gcatgcccgg gcggggcggg ccgatagcgg gccgcacgtg
     6361 atgctgagcc ccctggaggt gcgcccgctc ccgttccagg agcgcctgct ggagctcctg
     6421 caggtgtcac gccggcgcgg ccatcatcgc aacctcctcg tggccgccac gggcaccggc
     6481 aagaccgtga tggcggccct cgactacgcc gggctgcggg cacgcatgcc acgggcgcgg
     6541 ctcctcgtga tcgcgcaccg cgaggagata ctcgaccaga gcctcgcgac cttccggcac
     6601 gtgctgcgcg cgccgtcgtt cggtgagaag tgggtcgggg gagcgcgccc gacgcgcttc
     6661 gagcacgtct tcgcctcgat ccagagcctg aacgcggcgg acctcgacaa cctgccgtcc
     6721 gaccacttcg acgtggtcat cgtcgacgag ttccaccacg ctgcggcgac gtcgtaccgc
     6781 cgcgtgctgg accatcttgc gccggtggag ttgctcggcc tgacggccac ccccgagcgc
     6841 agcgacggcc tgccggtcct gcactggttc gaggaccgca tcgccgccga gctccgcctg
     6901 tgggacgcga tcgaccagca gcacctcgtc cccttcacct acttcggcgt gcacgacaat
     6961 ctcgaccttc gccgcgtgcc gtggcgccgc gggcgcggct acgacctcga cgcgctgacg
     7021 aacgtctaca cgtcggacga cgcatgggcg cgccttgtgg tggcccaggt ccacgagcac
     7081 gtggacgccg ccggcatgcg cgccctgggg ttctgcgtca gcatcgacca cgcgcggttc
     7141 atggcccggc acttcacccg ccatggcatc cccgcggtcg cgatctgggg agacagcgct
     7201 cgcgagcagc gcgagcaggc gctgcgtgac cttgccgcgg gacgcgtgcg ctgcgtgttc
     7261 tcggtggacc tgttcaacga gggcatcgac gtgccggcgg tcgacaccgt cctgatgctg
     7321 cgaccgaccg agagcccggt gctgttcctt cagcaactcg ggcgcggcct gcgccgatcc
     7381 gacggcaagt cggtgtgcac ggtgctcgac ttcgtcggtc agcaccggca cgagttccgc
     7441 ttcgatcgcc gtctgcgggc cctcctcggc ggcacgcgca ccgacgtcga gcgccaggtg
     7501 aaggagggct ttccctacct gccggccggc tgccacatgt acctcgaccc ggtcgccgcc
     7561 gacattgtgc tgcggagcct cagggaggcc atccccagcc gctgggccgc gaaggtcgac
     7621 gagctgcgat ccctgcgacg cgacctcgga tatgtcgacc tggcacggtt cctcgccgtg
     7681 tccggtctgg atctggacga cgtgtacgag gcacagaggg gctggtccga cctgcagcag
     7741 gccgccggcg tctccgtcgc cgccgcggga ccacatgaga ggcccctgcg gcgcggcgtc
     7801 ggccgtctgc tgcatgtgga cgatgaggag cggctcggca cctaccgccg cctcctcgca
     7861 ggcgaccggg ctccccaggc cgaacgcctg ggcgagcggg agcggcgcct cgcgcggatg
     7921 ctggtcgccg tggtgaccga caccgccctc gaccggcaga ccaccctcca gcaggcgctc
     7981 gacctgctct ggcgccaccc gcaggcgctg gccgatttgc gggacctcgt cgccgtgctc
     8041 cgggagcgga tcgatcacgc gcacacaccc ctcgccggcc acccccacgt gcccctgcag
     8101 gtgcacgccc ggtacagccg gctcgagatc ctggccgcga tgggggtggg tcacggcgcg
     8161 aaggtcgccc cgtggcagac cggcgtgtac gaggcgcgca gcgcgcgggc ggacctgctg
     8221 gcattcacgc tggacaagag cggccggcgc ttctcaccga cgacgcgcta tcgcgactac
     8281 gcgatcagcc gcacgctgat ccactgggag agccagtcgg tgacgcgcgc cgacagcacc
     8341 accggccgtc gctaccgcgg acatgccgaa ctcggctggt cgatccttct gttcacacgg
     8401 ctcaacgtga acgagcgcgc tttctggttc ctcggcccgg cgacttaccg gacccaccag
     8461 ggcgagcggc caatggccat cacctgggag ctggcccacc ccctccccgg cgacctgtac
     8521 gcccgcttcg cggctgccgt cgcgtaagtg gcgggctccg gcggagtgaa tgtccaacga
     8581 gcgagggatc ccggcggtcg gcacacgtaa ttcgatcgct ccgagcgacc gatggggaag
     8641 aaccggcacc tggtgacccc gccgcgggga tgaacagctt aggggtgccc gcgcgggtgg
     8701 cgaagcggcg ctcgcgaaag ccattgcggt gggtgcccgc tcggccggcg cccgttcgct
     8761 gtgggcggcg ccggtctcgg cggagacctc ggcctccatg atctggtggc ccagaaaggc
     8821 tcaccgcctc gcgcaccagg tcggcgtgct cgtcggtccg gaccttgcgc ctagagcgcg
     8881 tcggcgtcga ggtggcgacg gtgggcctcg cgggtctcct cctcgttcgg cgcgtgggga
     8941 tgaaccgacg ctgggagctc gcggtggcca gccctgcctt cagcaggtgc cgctcctaca
     9001 ccacagcacg gggcgcgatg gcaccgcgac gcttccgggt ggcaacccag cgaaaagccg
     9061 aagatgccga cgactatggc cgcgaaggag cgacgtcacc gacggttagc gcgcaaggga
     9121 ggggttcggc gaagagtcga acgtgcggat gcaccagaca gggctgatcg cacaactgtc
     9181 tcgattgtgg gaacgtcggc gtcgacgacc atggtggttc tgggtgactc ggacaagccc
     9241 aggaagaccc acgcgcggtc cacgcggacg gtcccggccc gcagggcggc gaggctgtaa
     9301 agcgccgcct gcagttcgta ctccccgcgc aggccgttcg gttgcttacc gtcgagcgcg
     9361 tgcgtcttcc agtccaccac gagaagcgtt ccgtcaggca gccacgcaag caggtcgatc
     9421 gcgcctgcca ggagaacacc gtcgaccacg accgcgaagg ggtgctcggt ctccactcga
     9481 agggcggtgc ggatacgctc ggcgagcgga ctggcgagca cgtcgcggac aagcgactcg
     9541 gcgcgctcgg cgtgagccgg tggcagcggc cccaggccga gcgaggctgc gagcggcgcc
     9601 gcccacccgg ggggcggcgc cgttgccgag tgcggaccga gccaccggtg gcgctccagt
     9661 gcgacgtgga cgagagtccc gacgaccgct ccacctccgt cggcggcggg aacagcgccc
     9721 gagtgcgccc cctctccagg cgcctcgtgc agggcctgta gcgccgtgaa cgacagccgc
     9781 cgaccggcga gggcatcgac ggacaaaggc tcggcaggga ccggagcgtg cgcggccgcg
     9841 accggtggcg cggcgggcgc gggccgacgc tcggggacta cgatcgacgc cgccgcgacg
     9901 gcggtctccg cggcgaacgc gctgccggcg tcgatcgcct gtttgagcag gctccacatg
     9961 cagttggggc cgccgcggcc gtcgggccgt cccacgacgg tgacgtgccg cttggcgcgc
    10021 gtgagggcga cgtagaagac gcgccgctcc tcggcggcgg cttcggcctg gaggcgctcc
    10081 ttcaggtccg ccgcgccgaa caggtccacc gggtctcccc cggcgagctt ggcccggaag
    10141 cccgccctgc cgtcggtgtc gaccatgagc aggtcctgct gcccgggcat gccgtgggac
    10201 ccgtcggcga tgacgacagc cgggaactcc tggcccttga cggcgtggat ggtggtgagg
    10261 cgcacggcgt cgaggtccgg gtcgacgacg accgcctcgc cggggtcctg ccccagccgg
    10321 tcgcgttcgg cgagggcatg caggaagccg gggagatccg gcccccgcac ctcggcgaac
    10381 gactcggcca tttgcacgag gcggcggagg ttggcgaagc gccggcggcc gccctccagg
    10441 ccgagcaccg ccaggtcgta cccgttggcg gcgatcgcgg cctcgacgag cgccgggagt
    10501 ccgctctcgc ggagcacggg gcgcagcgct gcgaccaagt cgaccagcgg ggcgagggcg
    10561 ggtatgccgg gcaccgccgg ccagaggggc cgcttggtcc ccgcgtgccg ccggagcgca
    10621 acgagatcgc cgtcgctcgc gttgacgtac ggcgatccca gcacgcgtac gaggtggtcc
    10681 tcgtggtgcg ggttgtcgac cagatggagc agggcgagca catcggcgac ctgctgatgc
    10741 gagaagaagc cgcgcccggc gacgaggtac gtcgggatgc cccgcacacg cagggcgcgc
    10801 tcgtagggct cgaccatggt gagggcgcgg aacagcacgg cgacgtcccg gttggcgtac
    10861 ccgtcgtgca gaaggccctc gacggcgtcg gccaccgcct cggcctcctg gtcgcgcgtc
    10921 gagcgctctc tgtccgcatc gctcacggag acgagctcga tgggcgggcg ccgcagtggg
    10981 ggcgcgggcg tcgttgccgc cgtcgcgtgc agagagcgga acgcgggctc cccggcgagg
    11041 gcgtgcgcga agacgtcgtt gagcgcctcc atcagctccg gctgggagcg gtggttgtcg
    11101 cggagcgcgt agcgcggagg gtcgcccacg cgctcggcga agaagcgcga ggtcgcgtgc
    11161 cggaagccgt agatggcctg gcccccgtcg cccaccaccc aagtgcggcg ggcgccgagg
    11221 gcgtcgacga tctcgttctg gatcgcgttg gcgtcctgga actcgtccac atagacccgg
    11281 tccagaccaa ggctcactcc ggcgcgcagc acgtccagcg cgtggaactg caggtcctcg
    11341 tagtcgagcg cgccgagctc caccttgcgc tgcgcgtaac cggtggcgaa ccgttccatc
    11401 acctcggcga gcgcctccag ttgcgggcgg gcgaacaggg cgacccggtc cgtccagatg
    11461 cggcacagca gctcgttggc gtcgtcgatg agcggcttga cgcccttctt ggccgtgccg
    11521 tggatctttg ggcacttggg gagtggcccc tccccgctca acagcacgcg ccccgccgcg
    11581 atgccggcgg cgtgccgctc gtgcaggtcc gggtggacaa ccagctcgtc gagcagccgc
    11641 gctgcgccga cggcgtcggc ggggtcgagc accggcggcg gggggagcac gaggcgaggc
    11701 tcgcgatgcc cggccgcgcg caggcgcgcg taggccgaca gcaccgcctg gcggatgcct
    11761 ccgtcgtaga cggcctgtgc gagtgcgtcg aggagcccgg ggtgggtggt cgcggccagg
    11821 gcgtcgtcga gggcctgctc caccagcgcg tccgcctcga cgtcgtcgag ctcgctgaac
    11881 tcgggatcca cgcccgccag gaacgggtgc atccgcacga tgcgcgccga cagcccatgg
    11941 aaggtgccga tccagccggc ggagaggtcg aggcttgccc gatcactgga tcggcccgca
    12001 tcgcggtccc caaagcgctg ctgcaggcgg tcgcgcaggt gcgccgcggc ggaccggttg
    12061 aacgtcacca cgaggatgcg ctcgatcggg acgccctcgt gctccacatc gcgccagatg
    12121 cgttcgacga gcagtgtcgt cttgcccgca cccgcgcccg cggccaccgg cacgaacggc
    12181 tcggacgcct cgagcacacg agcctgctcg ggtgtgggcg gcatcaccgc catagcgcac
    12241 cgcacccgca ccacctcgcg cagcccgcgc tcggcgggcc ggcgagccgg cctccccgca
    12301 gatccgcggc cacctggcgg gccgcgtcga tcgcctcgtc gaccatgccc cgccattcgc
    12361 ccgcatcgac ggaccgcggc ggccgctcgg cgtaatccgc gtcgctccgc accagcccgt
    12421 cgcgccgccc cccgagcagc gatacgtaga ggcccccggc gctctccaag ccgcgttcgg
    12481 ccgcggcaag gtacccgtag aggggtggct gcagccgctt cggcaactcc tcggtaccgc
    12541 ggtagtcccg cccgctgcgc ttgtagtcga ccaccaccgc gtgccggtgg tccgcgtcga
    12601 gccggtcgag gcggccggtg agcgcaagcg gcgggtgcac gtccagcagc gcctcgtcgc
    12661 gaagggcatg ctccacggcg accgggcgca tccggggacc cgaggcggcc tcattggcca
    12721 gcagcagctc caggtgcacg gccagtcggg tgccgaacgc gtcgaaggcc gtcgtgctgc
    12781 gattcggcct ctcccgtacc agcacctcgc ggaggcagtc cgccagcgcg gcccggtacg
    12841 ccggcaggcg cgcgaggtcg caggggccag gatcgggtcc ggccgcaacg cgggcgtaga
    12901 cggcggccat gatctcgtga ccgatcgtgc cctccgcggc cgggtcgaag ggccgcacca
    12961 gcggatccgg ggacaggtac cggccatgga gccacccgta cgggcagccg aggaacgtct
    13021 ccacgccggt gaccgagatc tcggtgacgt ggtcgagggc ccccgggtgg aggccgagcg
    13081 gcctcacccg ccgcgccgcg gcgttggcga gcagcccggg cacggcgagg ccgtccaggg
    13141 ccagccggcg cagcatctcg cgctccggtc ccgcctcatc gcgcgccagg atctccccgt
    13201 cggggtccgg gcggacgtcg agcccgtcag cgtcgaggcc cgccgcccgg catgtctcca
    13261 tccagaaggg cgagggggcg acggggcggc cctcgtcgtc ggccacccgg cgcaccagga
    13321 tcacctggtc ggtggccgcc gccagggcgt ggacgaaccg gagtcgggcc tccgacgtgc
    13381 cgggggcgcg cggaacgagg cgctcgggaa gcacggcccg gaaggcgccg agcagcgcgt
    13441 ccggggcggg accggacgga agtccgtcgc cgtccagacc gcagagagcg accgtgtgga
    13501 acacgcgccc ccgcagcgac gcgtagtcga gcacggccag gcccgcctcc ggaccgcgct
    13561 cgtccgggac ggtgaggtcg gcgatcagcg caccgagttc gcccgaggga agcgggccgc
    13621 gtgcacgcgg cagcgcatcg tcctcgaggg gcacatcgtg caggtcagct gccaggccgg
    13681 cgagcgcggc gagcgcacgc cggtcgagct cgtcggcatg cgtgggcgga gcctcgggca
    13741 gcgccgccag agcgccctcg cccaccgcca ccagcgcggc cagctgcgcc cgcacgccgt
    13801 cgcgggcggc ggcgaccagc cgccgcgccg gtaccgcgac ctcgccccac cgtccgatca
    13861 gctcccgccg ctgatcgagg cccgcgtgcc gcgcgtcgcg ctcgaacgcg tcgacctccc
    13921 ccgggtcggc cccggagtac ggcgagcgca gccagccgag gagcgccgcc aatgcccccg
    13981 atgcgtcgcg atcgagctcg ctgaaccgca gcaggtgcac cagcgcgagc cccagtcgcg
    14041 tctccagcac gcgccggcgg gtgaggcgcc gggcaggcac ctcccagtcg gcgagcaggc
    14101 gatcgaggtc gtcgcgtgct cgggacagcc ccggcaccac gagggcgcac tgcgcggggc
    14161 gtactccgcc gcgaatcgcc tgcagcaggg cggagagggc ggcccggtgc atgccgcggg
    14221 ttcctgcggc gccgatgcga tgcacggcgc gcccatccgc gaccggaggc cggtcctcga
    14281 agacgacgtc ctcgatacga cctccgccgc ctgcgcggtg cgtcgtcacc tggtccgcct
    14341 gcgcgcccca ccccgcctgg cgcgcgtgtc gggcctcatg cccgcgtcgg gcgtcgtcgt
    14401 agggcatcac caggtgcacc tcggtgacgc gcgcgagcgc ccgcaggagc gaccaggcgc
    14461 tcggcgccag gtcatcgatg ccacaggcga cgacgggacg gaactccgtg agggcgtcgg
    14521 cctggcgcac tgcagaccaa cgcacgtcgg cggggtgggc ggcgtcggcg gccgcggcga
    14581 gcaccgcggc gatctcggcc gacaccggat ccggggctct ggtcgcaagt tcggtcgcag
    14641 ccagaccggc ctcgcggatc tccgccaggg cacgctccag cgccgtcagg aacccggggc
    14701 cccgcgcgga ggcgcccaga cgctcgagag gcgtccggcg gatcgcgtcc tgcagagcga
    14761 gccggacggc gacgcgggag ggcacggcgc gaggcggatg ctccgccacg cgcagcaccc
    14821 ggtcccggag tgcggcccac gtgaccgcct cccggtcgaa caggacaccg ctggggccca
    14881 ccgcgcgaat gagttcccgg gtgacggcgt ggcggtccgg cgcgacgagg aaaccgttgc
    14941 cggtggcggt ccacagctcg tgggcgacgt cccacgcgtg ggcgaacgtg gcggcgcggt
    15001 gaagcgtcag cggcacgtgg ccgagtgttg cggacgccct ggacggcgag gccttcccac
    15061 aggagctcgt cgcccagcac acggccccca gggccccgtc accgcagttc gtccgatcac
    15121 ctggtcacgg tgtctcactc gggggacgct gaggcgcacg tgtctactcg caggcgtagc
    15181 cgtcgccgtc cctgtcgagc ccgaatcggt ccggtcctac gacttggacg gggccggtgt
    15241 agtcgggacc gtctccgctt ccgcccgcgc agtcgtagtc cggcgaggac gggtcgaggc
    15301 acgcgccctg atagctcggg tcgcattcac tcgacgaggc cgtggtcggc tcgggtggag
    15361 tcggtctggc aagttgggcc tgccgttcgc gcacctggcg ccgcagtacc gagatcgcgg
    15421 gtaggggcga ccagccctcc gccctgccca acttcgcgag ggcttgacca ggtcggtcat
    15481 tctcgaggtc acgccgcgcc tgcgagacga gcacaagcgc gtgctttctc gagtacgccc
    15541 gcgccttctg gggcgcgtca cgaaagccgc caagggcctt catggtccga gccgcgcgcg
    15601 catagcgtcc accgcggccg gagcgctcgc ccgcgaggta tcgccgggtc cggtcgacga
    15661 tgacccgagc gcgcgcgatc tcctgcgcga cgacgggatc ttgcgcctcg atctccgcgg
    15721 gcacccgcgc cagatagccc tcggcgacgt cgaggcggtc ccgcgccgcc gcccgctccg
    15781 cgcgtgccag cagcagacgc gcccgttggg cgggggcggg tcgcgtcgcc gcggcgatgg
    15841 tccgaggcgt cgtcccggga gccgtcgacg cggaggtctc gtcacgcggg gcgctcaggg
    15901 tggcgaccac cacgaccagg gcaacaacgg ccagggccgc gataaccagg aatctcttcg
    15961 tggggcgctt accgttcggg ggctggattg cctctcctgc cggcccggcc ggcgtgcgcg
    16021 ggggccgcgg ctcagtcggc agaggaggtg ccgaggcgac gcgagcaacc ctgtgcgccg
    16081 acgtgagcag gatcagatcg tccagcgcgg ccgtcgtgaa cgcgggatcg acggtgagtc
    16141 ccgcgtcgac gagcgccgtg atgatgcgct gccgccccgc gagagtgagc cggctcagac
    16201 caaacgtgtc ccggatttct cgcacggaca ctgcgagccg cccgccgtgc tgctcgacgc
    16261 gatgccggat cgtcaccgct ggatcgcgca cccgcccacc acccaccatg tcaacgccca
    16321 ctcgccgctg accaggctag tggcggatgt tcgcccattg acctggtcga cccggcaggg
    16381 gtgctgccgg tcgtatggct cctggtcgta tgccctccgc cacagagcgg cgacaagaac
    16441 agacgtccgc gcttcccaca tccgtcagag cccggatcgc tcccgagacc cgagtcgttt
    16501 ctggcggttc acacgacgtc ggccacgcag tggacctccg tcaggacgtc ggcatcgccc
    16561 ccatcg
//
LOCUS       DATMKC010000335         3371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_6258, whole genome shotgun sequence.
ACCESSION   DATMKC010000335 DATMKC010000000
VERSION     DATMKC010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6258"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VNU01_09905"
     CDS             complement(<1..478)
                     /locus_tag="VNU01_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWH32971.1"
                     /translation="MGVVSEHRRGLAALFGAVLIWSSTYVLIKGMLDDIGPFTLAVAR
                     FAVALAVLAPLAARRGFRWRMALERRWLAFGAAGVTLYFGLQNLGLVFTTAGSAALIT
                     ASIPALTALVARLSLGERLRRRQAAGVTLSVVGVALVVRSGLELGDARTLAGNVLIL"
     gene            660..1709
                     /locus_tag="VNU01_09910"
     CDS             660..1709
                     /locus_tag="VNU01_09910"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HWH32972.1"
                     /translation="MSAQPSHAQSSHALPDAVLPPDAESIMEQALRQEGAPEVKGVRP
                     TTAEELRFQRRMAVIFTVLPLAGVVAAMALLWGRGISPLDFWIFLGFYLFTGFGVTVG
                     YHRLFTHRSFDVPKPVRAVIGVAGSMAVEGSLIDWVSTHRRHHAYADAYGDPHSPHLV
                     EEQGLRGVLRGLWHAHMGWMFEPNGTMNDRWAPDLQEERLTARIDKAFPLLTVLTFAL
                     PPLLGFVLSGFSLWAAFTAFLWGSLVRVFLLHHVTWSINSICHFFGDRPFDSRDESTN
                     NWPLSLLSFGESWHNNHHAFPTSARHGLLRFQFDPSWRVIRTLEQLKLARDVRLPSRA
                     VLAKKWAGRPQRLAR"
     gene            1757..2377
                     /locus_tag="VNU01_09915"
     CDS             1757..2377
                     /locus_tag="VNU01_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH32973.1"
                     /translation="MSEGSGRARARMTGTQRREQLIGIGRQVFAERGFEAASVEEIAE
                     RAAVSKPVVYEHFGGKEGLYAVVVDREVRLLVDEITQAIEATAHPRDAVEGAALAFLT
                     YIEEHREGFQILVRDAPIGTTTGTLPSVLGDVAASAESVLAAQFKSRGYETKTAPLYA
                     RALVGMVALVGQWWLEAGKPKRREVVAHIVNLAWNGLKDLDPAPKP"
     gene            complement(2374..>3371)
                     /locus_tag="VNU01_09920"
     CDS             complement(2374..>3371)
                     /locus_tag="VNU01_09920"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH32974.1"
                     /translation="PRVGGATRDAAGIAIQVSRAVFPDAAGDAAAAAPKARHVVLARD
                     DTWADSLAGTALSGDWGPILYARGGPAGTLPDETLAELRRVLGPGGRVYLLGGAGALS
                     PAVEQAVAAAGFAPKRLSGEERLRTALAIAAEVRALHPASGRVLVARAYDTPVDALAS
                     GAAAGHHHVPLVLSPTPGLTHQFLRDGVAGLGVPDATLVGGTAALSEQVAADLRALGL
                     RAERVSGPTRWHTAVAIADRFFPAPRTSVVAVDLESAPGWAWALAAGALSAMLDAPQL
                     GVAPSYAPTPTTDEIRSGGGTAAAPLRVVVVGDAERVSTAVRVALREAAEGAQAA"
BASE COUNT          457 a         1294 c         1221 g          399 t
ORIGIN      
        1 ccaggatcag gacgttgccg gccagggtgc gcgcgtcgcc cagctccagg ccggagcgca
       61 ccaccagcgc cacgcccacg accgacaagg tcacgccggc ggcctggcgg cgccgcaggc
      121 gctcgcccag cgacagccgc gccaccagcg ccgtgagcgc cgggatggac gccgtgatga
      181 gcgcggcgct gcccgccgtg gtgaacacca ggccgagatt ctgcaggccg aagtagagcg
      241 tgacgcccgc ggcgccgaag gcgagccagc ggcgctccag cgccatgcgc cagcggaagc
      301 cgcggcgcgc ggccagcggc gcaaggaccg ccagggcgac cgcgaagcgc gcgaccgcca
      361 gcgtgaacgg cccgatgtcg tcgagcatgc ccttgatgag cacgtaggtg gacgaccaga
      421 tcaggacggc gccgaacagg gccgccagcc cgcgccgatg ttcggagacc actcccatcc
      481 ggccatgatg ccgaccgcgg tgacccacac cggcggtcca cccctgcccg actggaccgg
      541 ccgccgcgga ccatggggac cggcgggccc gtaaacttac gctgtcgtag gttacgctcg
      601 cgtagccttg cgggaggctg actgtcccgc actccaccgc cgcagcacca ggagcacgca
      661 tgagcgcaca accctcccac gcacaatcgt cccacgccct gcccgacgcc gtcctgcccc
      721 ccgacgccga gtcgatcatg gagcaggccc tgcgccagga gggcgcgccc gaggtcaagg
      781 gcgtccggcc cacgaccgcc gaggagctgc gcttccagcg ccgcatggcc gtgatcttca
      841 cggtgctgcc gctggccggc gtggtcgccg ccatggcgct gctgtggggc cgcggcatca
      901 gcccgctgga cttctggatc ttcctgggct tctacctgtt caccggcttc ggcgtgaccg
      961 tcggctacca ccgcctgttc acccaccgca gcttcgacgt gcccaagccg gtgcgcgcgg
     1021 tgatcggcgt ggcgggctcc atggccgtgg agggctcgct gatcgactgg gtgtccaccc
     1081 accgtcgcca ccacgcctac gccgacgcct acggcgaccc gcactcgccg cacctggtgg
     1141 aggaacaggg ccttcgcggc gtgctgcgcg gcctgtggca cgcccacatg ggctggatgt
     1201 tcgagcccaa cggcaccatg aacgaccgct gggcccctga cctgcaggaa gagcggctga
     1261 ccgcccgcat cgacaaggcg ttccccctgc tgaccgtgct gacgttcgcg ctgccgccgc
     1321 tgctcggctt cgtcctgagc ggcttctcgc tgtgggccgc gttcaccgcg ttcctgtggg
     1381 gctcgctggt gcgggtgttc ctgctgcacc acgtcacctg gagcatcaac tcgatctgcc
     1441 acttcttcgg cgaccgcccc ttcgacagcc gcgacgagtc cacgaacaac tggccgctgt
     1501 cgctgctgag cttcggcgag tcctggcaca acaaccacca cgcgttcccc acgtccgcgc
     1561 gccacggcct gctgcgcttc cagttcgacc cgtcctggcg cgtgatccgc acgctggagc
     1621 agctcaagct ggcccgcgac gtgcgcctgc ccagccgggc cgtgctggcc aagaagtggg
     1681 cgggccggcc ccagcgactg gcgcgctgac gccggcgggg ggccgaccgg ccggcgacgt
     1741 gccatcatcg gcccccatga gcgagggcag cggccgcgcg cgagcgcgca tgacgggaac
     1801 ccagcgccgg gagcagctga tcggcatcgg ccggcaggtc ttcgcggagc gcggcttcga
     1861 ggcggcctcc gtggaggaga tcgcggagcg cgccgcggtc agcaagccgg tggtgtacga
     1921 gcacttcggc ggcaaggaag gcctgtacgc cgtcgtggtg gaccgcgagg tgcgcctgct
     1981 cgtggacgag atcacgcagg ctatcgaggc caccgcccat ccgcgcgacg cggtcgaggg
     2041 cgccgcgctg gcgttcctca cctacatcga ggagcaccgc gagggcttcc agatcctggt
     2101 ccgcgacgcc cccatcggca cgaccaccgg cacgctgccc agcgtcctgg gcgacgtcgc
     2161 ggccagcgcc gagtcggtgc tggccgccca gttcaagtcg cgcggctacg agaccaagac
     2221 cgcgccgctg tacgcgcgcg cgctggtcgg catggtggcg ctggtggggc agtggtggct
     2281 ggaggccggc aagcccaagc gccgggaggt cgtggcacac atcgtgaacc tggcctggaa
     2341 cggcctgaag gacctcgacc ccgcgccgaa gccctaggcc gcctgggcgc cttcggccgc
     2401 ctcgcgcagg gccacgcgca ccgccgtgct cacccgctcg gcgtcaccga ccaccaccac
     2461 gcgcagcggc gcggccgcgg tgccgccccc ggagcggatc tcgtccgtgg tgggcgtggg
     2521 ggcgtaggac ggcgccacac cgagctgcgg cgcgtcgagc atggccgaca gcgcgccggc
     2581 ggccagcgcc cacgcccagc ccggcgccga ctccaggtcg accgccacca cggacgtgcg
     2641 cggggcgggg aagaagcggt ccgcgatcgc cacggcggtg tgccaccgcg tcggcccgga
     2701 cacgcgctcc gcgcgcaggc ccagcgcgcg caggtcggcc gccacctgct cgctgagcgc
     2761 ggcggtgccg ccgacgagcg tggcgtcggg cacgccgagg ccggcgacgc cgtcgcgcag
     2821 gaactggtgg gtcagcccgg gtgtggggct gaggaccagg ggcacgtggt ggtggccggc
     2881 cgccgcgccg gacgcgagcg cgtccaccgg tgtgtcgtag gcgcgcgcca ccagcacgcg
     2941 cccggacgcc gggtgcagcg cgcgcacctc ggcggcgatg gccagcgcgg tgcgcaggcg
     3001 ctcctcgccg gacagccgct tgggcgcgaa gcccgcggcc gccaccgcct gctccaccgc
     3061 gggcgacagc gcgcccgcgc cgccgagcag gtagacgcgc ccgcccggcc ccagcacgcg
     3121 gcgcagctcg gccagcgtct cgtccggcaa ggtgccggcc gggccgccgc gggcgtacag
     3181 gatcgggccc cagtcgccgc tcagcgccgt gccggccagc gagtccgccc aggtgtcgtc
     3241 gcgtgccagc accacgtggc gggccttggg ggcggccgcc gccgcgtcgc ccgcggcgtc
     3301 ggggaacacc gcgcgcgaca cctggatggc gatgccggcc gcgtcgcgtg tcgcgccgcc
     3361 cacgcgcggc g
//
LOCUS       DATMKC010000336         4304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_290572, whole genome shotgun sequence.
ACCESSION   DATMKC010000336 DATMKC010000000
VERSION     DATMKC010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290572"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VNU01_09925"
     CDS             <1..1199
                     /locus_tag="VNU01_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32975.1"
                     /translation="ALGEAGVRPRDGRRQWSMHALAALTLLGGLSAFQGEFDFGVPQF
                     QLLYHPVLVLLSAAVALTAARAVLGRGGALVVAAGFLLFRGALMLFLQFGAERTVPRV
                     ALYLGIALVVEAVAAARPPERGMAPFALLTGAAVGTLGLATEWAWAAAVARHPWNAGL
                     VTEAVLVGLLAAVGGAVLGAGIAGGVRERLGVLPAALVVAGAVLGLASLLLPLPRTAR
                     MSPQAAVSYEHRGDEAKVRVVIGPPDAPYAARWWEVLAYQGGSMRVEPLLPGAGSGVY
                     ETAGTVPVGGDWKVALRLHKGTDILAAMLRRPADPEHGEEAIELRQGFITLDLDDPSG
                     EPAEGTPGWLGGVVLAAVTVLWLCWAWLTVRAAQASRAALAPPPAAQGKGRRGTKASH
                     RGSRAR"
     gene            complement(1207..2577)
                     /locus_tag="VNU01_09930"
     CDS             complement(1207..2577)
                     /locus_tag="VNU01_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWH32976.1"
                     /translation="MPRTARARVAPATYVLDTCVLLADPYALTRFDEHEVVLPLVVVE
                     ELDRNKTRLDEVGRNARLALRLIEDLRGASGSGLREAVALPTGGSLRVEGNHVDVLLP
                     PYLDPLKPDHRILAVALAIGGTLVTKDTALRIKGSQLGVAVEDYRADTAQVDEHYTGI
                     AELDVAAADLDALHRDGKAELSMDAVEGGFWMNQCLILRAGRSASGLARIVEVKEQEG
                     VAVATRVAGSPSAFGVAPRDVRQTFALDLLLDPAVPCVSLMGMAGTGKTFLALAAGLE
                     LTLEARAFRRLSVYRPLVAVGRQEVGYLPGDLDEKLEPWMAAVHDNLYSLFRRDDDAD
                     GWGRNGGTNHVRATIDSLIDRGQLEMAAITYLRGRSITDEYVIVDEAQNLELPTLKVI
                     LTRLAAGSKVVFCGDLSQVDNPYISPFGGMAALIEKLKGTALFGHVTMSKGVRSPLAE
                     LAATTL"
     gene            complement(2777..3340)
                     /locus_tag="VNU01_09935"
     CDS             complement(2777..3340)
                     /locus_tag="VNU01_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HWH32977.1"
                     /translation="MSVEKTDIEGLLVVRWDTHEDERGFFRQTYQVRELTEALGREPV
                     LRQGNHSHSVPGVLRGFHAEPWDKLVYVVRGIAMAAIADIRPDSPTFGEARRFMLGEP
                     PHGERIRLFISQGLANSFVTLGDTNVDYLYDVSDYWRPDIDKPAVIWNDPDLGVDWPI
                     QDPILSDADRANPTLRELFPDHPRWGR"
     gene            complement(3429..4205)
                     /gene="uppS"
                     /locus_tag="VNU01_09940"
     CDS             complement(3429..4205)
                     /gene="uppS"
                     /locus_tag="VNU01_09940"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWH32978.1"
                     /translation="MVHELAYRLYERRLAAGLRQAALPRHVAVILDGNRRYARARGLS
                     TVSEGHRRGAEKIHELLDWCDELGIPFVTIWLLSTDNLARDEQEMRSLSDIIAATVRR
                     MADEAPARKGGMAITAVGDLDALPGDLARTIKEAEEQTKGGQGLRVQVAVGYGGRQEI
                     TDALRGWLEARLEAGESLEQAIASLDEDAISAHLYTNGVPDPDLIIRTSGEIRLSGFL
                     MWQSAHSEFYFCDPYWPEFRKTDFLRALREFGSRSRRFGR"
BASE COUNT          531 a         1550 c         1611 g          612 t
ORIGIN      
        1 tcgccctcgg cgaggccggc gtgcggccgc gcgacgggcg gcggcagtgg tcgatgcacg
       61 cgctggccgc cctcacgctc ctgggcggcc tgtcggcgtt ccagggcgag ttcgacttcg
      121 gcgtgccgca gttccagctg ctgtaccacc cggtgctggt cctgctgtcg gccgcggtgg
      181 cgctcacggc cgcgcgcgcc gtcctgggcc gcgggggcgc gctcgtcgtg gcggccggct
      241 tcctgctgtt ccgcggcgcg ctcatgctgt tcctgcagtt cggtgccgag cgcaccgtgc
      301 cgcgcgtggc gctgtacctg ggcatcgccc tggtggtgga ggcggtggcg gcggcgcggc
      361 cgccggagcg cggcatggcg ccgttcgcgc tgctcacggg tgcggccgtg gggaccctgg
      421 ggctggcgac cgagtgggcg tgggcggccg ccgtggcgcg ccacccgtgg aacgccggcc
      481 tcgtcacgga ggcggtgctg gtcggactgc tcgccgccgt gggcggcgcg gtgctcggcg
      541 cgggcatcgc cgggggcgtc cgcgaacggc tgggggtcct gccggcggcg ctcgtggtgg
      601 ccggggccgt gctggggctg gcgtcgctgc tgctgccgct gccgcgcacg gcgcggatgt
      661 cgccccaggc cgcggtgtcc tacgagcacc ggggtgacga ggccaaggtc cgggtggtga
      721 tcgggccgcc cgacgcgccc tacgccgcgc gctggtggga ggtactggcc taccagggcg
      781 gcagcatgcg cgtcgagccg ctgctgcccg gggcaggctc aggcgtctac gagaccgccg
      841 gcacggtgcc ggtcgggggc gactggaagg tcgcgctgcg cctgcacaag ggcaccgaca
      901 tcctggcggc catgctgcgg cgcccggcgg accccgagca cggcgaggaa gccatcgagc
      961 tgcgccaggg cttcatcacc ctggacctgg acgaccccag cggcgagccc gccgagggca
     1021 cgcccgggtg gctcggcggc gtggtgctcg ccgccgtgac ggtcctctgg ctgtgctggg
     1081 cgtggctcac cgtgcgcgcc gcgcaggcgt cccgcgccgc cctggcgccg ccgccggccg
     1141 cccagggcaa gggccgccgc ggcaccaagg cgagccacag gggctcacgc gcccgctgag
     1201 ccgctgctac agcgtcgtgg cggccagctc cgcaaggggt gagcgcacgc ccttgctcat
     1261 ggtcacgtgc ccgaacagcg ccgtgccctt gagcttctcg atcagcgcgg ccatcccgcc
     1321 gaacggggag atgtaggggt tgtcgacctg ggacaggtcg ccgcagaaca ccaccttgga
     1381 gccggcggca aggcgggtca ggatcacctt cagcgtcggc agctccaggt tctgggcctc
     1441 gtccacgatc acgtactcgt cggtgatcga acgcccgcgc aggtacgtga tagcggccat
     1501 ctccagctgg ccgcggtcga tcagcgagtc gatcgtggcg cgcacgtggt tggtgccgcc
     1561 gttgcgtccc cagccgtcgg cgtcgtcgtc gcggcggaac agcgagtaca ggttgtcgtg
     1621 cacggccgcc atccacggct cgagcttctc gtcgaggtcg ccgggcaggt agccgacctc
     1681 ctggcgcccc acggccacca gcggccggta caccgacagg cgccggaacg cccgcgcctc
     1741 cagcgtcagc tccagcccgg ccgccagcgc caggaacgtc ttgccggtgc cggccatgcc
     1801 catgagcgac acgcacggca ccgccgggtc cagcaggagg tccagggcga aggtctggcg
     1861 cacgtcgcgc ggggcgacgc cgaacgcgct cgggctgccc gcgacgcgcg tggccacggc
     1921 gacgccctcc tgctccttga cctccacgat gcgcgccagc ccgctggccg accgcccggc
     1981 gcgcaggatc aggcactggt tcatccagaa gccgccctcc acggcgtcca tggacagctc
     2041 cgccttgccg tcgcggtgca gcgcgtcgag gtccgccgcc gcgacgtcca gctccgcgat
     2101 gccggtgtag tgctcgtcca cctgggcggt gtcggcgcgg tagtcctcca ccgcgacgcc
     2161 gagctggctg cccttgatgc gcagcgcggt gtccttggtg accagcgtgc cgccgatggc
     2221 cagcgccacg gcgaggatgc ggtggtccgg cttcagcggg tccaggtacg gcggcaacag
     2281 cacgtcgacg tggttgccct ccacgcgcag gctgcccccg gtgggcagcg cgacggcctc
     2341 gcgcaggccg ctgccgctcg cgccgcgcag gtcctcgatc aggcgcagcg ccaggcgggc
     2401 gttgcgcccg acctcgtcga gacgcgtctt gttgcggtcg agctcctcca ccaccaccag
     2461 cggcagcacg acctcgtgct cgtcgaagcg cgtcagcgcg taggggtcgg cgagcagcac
     2521 gcaggtgtcc agcacgtagg tggcgggggc gacgcgggcg cgagcggtcc tcggcatgcg
     2581 ggcggtctcc tgcttcagga aaacgaagaa cgcgccccgg ctggggcgcg ttcagggact
     2641 ggcttgcgac cgtcgcggac cgccggccgc gttgccggct ggtacaggcg ccatccgttc
     2701 gtcgtcaccc ggatcctcgg tcgcgttcga ctgcgaaggc gaggatacga ccgcctcgcg
     2761 gcggaacggc ggaggctcag cggccccatc tcgggtggtc tgggaacaac tccctgagcg
     2821 tcgggttggc ccggtcggcg tccgacagga tcgggtcctg gatcggccag tcgaccccga
     2881 ggtccgggtc gttccagatc acggcgggct tgtcgatgtc cggccgccag tagtcgctga
     2941 cgtcgtagag atagtcgacg ttcgtgtcgc ccagcgtgac gaagctgttg gccagcccct
     3001 gggagatgaa caggcggatg cgctcgccgt gcgggggctc gcccagcatg aacctgcgcg
     3061 cctcgccgaa cgtgggcgag tccgggcgga tgtcggcgat ggccgccatg gcgatgccgc
     3121 gcacgacata gaccagcttg tcccacggct cggcgtgaaa gccgcgcaga acgcccggca
     3181 cgctgtggct gtggttgccc tggcgcagca ccggctcacg ccccagcgcc tcggtcagct
     3241 cgcggacctg gtaggtctgg cggaagaagc cgcgttcgtc ctcatgggtg tcccagcgga
     3301 cgacgagcag gccctcgatg tcagtcttct cgacgctcat ggcgcgccag gctagcggcc
     3361 gccgcggcaa acgggtgccc ccattgcggg atgtcgcggt gcccgcaccc gttcgaacgg
     3421 ggaacgggct agcggccgaa gcggcgcgag cgggagccga actcgcgcag ggcgcgcagg
     3481 aagtcggtct tgcgaaactc cggccagtac gggtcgcaga agtagaactc gctgtgggcc
     3541 gactgccaca tcaggaagcc cgacagccgg atctcgcccg acgtgcggat gatcaggtca
     3601 gggtcgggca cgccgttggt gtacaggtgg gcgctgatcg cgtcctcgtc cagcgacgcg
     3661 atcgcctgct ccagggactc ccccgcctcc agcctggcct ccagccagcc gcgcagcgcg
     3721 tcggtgatct cctggcggcc gccgtagccg accgcgacct gcacccgcag gccctggccg
     3781 cccttggtct gctcctcggc ttccttgatg gtccgggcca ggtcgcccgg cagcgcgtcg
     3841 aggtcgccga ccgccgtgat cgccatgccg cccttgcgcg ccggcgcctc gtcggccatg
     3901 cgccgcaccg tcgcggcgat gatgtcggac agcgagcgca tctcctgctc gtcgcgcgcc
     3961 aggttgtccg tcgacagcag ccagatggtg acgaagggga tgcccagctc gtcgcaccag
     4021 tccagcagct cgtggatctt ctcggcgccg cggcgatgcc cctcgctgac ggtggacagg
     4081 ccccgggcgc gggcgtagcg gcggttgccg tccaggatga ccgcgacgtg ccggggaagc
     4141 gccgcctgcc gcagtcccgc ggcgagccgc cgctcgtaca gccggtaggc gagctcgtgc
     4201 accatccgcg gagtctagcc agccgggtgg gcgacgccct ccggagcagg ggtgtccgac
     4261 cgggcgggca gcgtctcgcg catgcgcagc gcccacaccg cggc
//
LOCUS       DATMKC010000337         2494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_96359, whole genome shotgun sequence.
ACCESSION   DATMKC010000337 DATMKC010000000
VERSION     DATMKC010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96359"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..608)
                     /locus_tag="VNU01_09945"
     CDS             complement(138..608)
                     /locus_tag="VNU01_09945"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWH32979.1"
                     /translation="MAVPRSRLRLTDEELERFLTEQRTLRLATVGDDGVPHVVPLWFV
                     WHDGAIWLNSLAKSERHGHLRAGRPVGLVLDDGERYEELRGVRMSGRPEIVADDDPVR
                     VEAYRRFARKYFGVDELPNQRSYETVRIVPDEVASWDFRKIPSGADKKVGLSKD"
     gene            complement(620..1531)
                     /locus_tag="VNU01_09950"
     CDS             complement(620..1531)
                     /locus_tag="VNU01_09950"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HWH32980.1"
                     /translation="MDLGYSGALYILAFDHRGSFQKKMFGIAGEPTAEETAKITDAKR
                     LIYEGMLKAAEGDVEPSELGVLVDEQFGGSTPADAKASGLKLAMPVEKSGQDVFDFQY
                     GDDFGAHITSFDPDFSKVLVRYNPAGDAQSNADQLGRLKRLSDWLHANDRKFLFELLV
                     PAEPAQLEAVGGSEERYDAEVRPDLMVQAIKEIQDAGVEPDIWKIEGVDERADCERIA
                     EQTRDGAGRENVVCVLLGRGANDAKVDHWLTQAAPVEGFVGFAIGRSIWWDALKGYLD
                     GSLEREKAAQQIADNYLRFIDVYKRAR"
     gene            1755..1958
                     /locus_tag="VNU01_09955"
     CDS             1755..1958
                     /locus_tag="VNU01_09955"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWH32981.1"
                     /translation="MAVIKTIDLVGVSTESWRDAAMKALEEASKTLRGIEGFDVLETS
                     ATVSENLVAEYHTHIRIRFRIDR"
     gene            1967..>2494
                     /locus_tag="VNU01_09960"
     CDS             1967..>2494
                     /locus_tag="VNU01_09960"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWH32982.1"
                     /translation="MPALILVGTQWGDEGKGKATDLLADQVSHVVRYQGGNNAGHTII
                     VGDVTLKLHLVPSGVMYPHVTPVIGDGVVLDPGVLLDELDALVAQGLDVSKLKISGNA
                     HLIMPYHRELDAVTERYLGRQKLGTTKRGIGPAYADKAARVGLRVQDLYDMKIFRQKL
                     DTVLKERNAVLSRVYN"
BASE COUNT          368 a          907 c          838 g          381 t
ORIGIN      
        1 ggtcgcgagc tgggacttcc gcaagatccc ctccggcgcc atgagcacgg acggcttcca
       61 cccggcggcc accggctacg gcttctgggc ggacgccctc gccgccgggg tggtcggcgc
      121 cctgccctga tccggggcta gtccttggac aagccgacct tcttgtcggc gccggagggg
      181 atcttgcgga agtcccagct cgcgacctcg tccggcacga tgcggaccgt ctcgtagctg
      241 cgctggttgg gcagctcgtc cacgccgaag tacttgcgcg cgaacctgcg gtaggcctcg
      301 acgcgcaccg ggtcgtcgtc cgccacgatc tccggacgcc cgctcatgcg cacgccgcgc
      361 aactcctcgt agcgctcgcc gtcgtcgagg accaggccca ccggccgccc ggcgcgcagg
      421 tggccgtgcc gctcggactt ggccagcgag ttcagccaga tcgcgccgtc gtgccagacg
      481 aaccacagcg ggacgacgtg cggcacgccg tcgtcgccca ccgtggccag gcgcagggtg
      541 cgctgctcgg tgaggaaccg ctccagctcc tcgtcggtga gccgcagccg ggaccggggg
      601 accgccacgt ccggccgccc taccgggcgc gcttgtagac gtcgatgaag cgcaggtagt
      661 tgtcggcgat ctgctgcgcc gccttctcgc gctccagcga cccgtccagg tagcccttga
      721 gcgcgtccca ccagatcgag cgcccgatgg cgaagccgac gaagccctcg acgggcgcgg
      781 cctgcgtgag ccagtggtcg accttggcgt cgttggcacc gcggcccagg agcacgcaga
      841 ccacgttctc gcggccggcg ccgtcgcggg tctgctcggc gatgcgctcg cagtcggcgc
      901 gctcgtccac accctcgatc ttccagatgt ccggctcgac gccggcgtcc tggatctcct
      961 tgatcgcctg gaccatgagg tcggggcgca cctcggcgtc gtagcgctcc tcgctgccgc
     1021 cgacggcttc cagttgcgcc ggctccgccg ggaccagcag ctcgaacagg aacttgcggt
     1081 cgttggcgtg cagccagtcc gacaggcgct tgagccgccc gagctggtcg gcgttggact
     1141 gcgcgtcccc ggcggggttg tagcgcacca ggaccttgga gaagtccggg tcgaaggacg
     1201 tgatgtgcgc gccgaagtcg tcgccgtact ggaagtcgaa gacgtcctgg ccgctctttt
     1261 cgaccggcat cgcgagcttc aggcccgacg ccttcgcgtc ggccggcgtc gagccgccga
     1321 actgctcgtc caccaggacg ccgagctcgg acggctccac gtcgccctcg gcggccttga
     1381 gcatgccctc gtagatcagg cgcttggcgt cggtgatctt cgcggtctcc tccgcggttg
     1441 gctcccccgc gatcccgaac atcttcttct ggaaggagcc gcggtggtcg aacgccagga
     1501 tgtacagcgc gccggagtag ccaaggtcca ttctcgagca ccagccttgt cgggtcggtc
     1561 atgacggttt gcggctcact ctacccagca ggcccccggg gctcccattc gccgggcgcg
     1621 cgcgcgaggt ggcggcccgc cgtccccggc gactaccatc ccccaggcat ccccccaagg
     1681 cgtccccggg actcccgctt ggtcaccggc gctcacccgg gcggagcctt gcgaaacgac
     1741 aggagcggta caccatggcg gtcatcaaga ccatcgacct cgtcggcgtc tccacggagt
     1801 cctggcgcga cgcggcgatg aaggcgctgg aagaggcgtc caagacgttg cgcggcatcg
     1861 agggcttcga cgtgctggag acctccgcga cggtctcgga gaacctcgtc gccgagtacc
     1921 acacccacat ccgcatccgc ttccgcatcg accgctagga cgcgccatgc cggcactcat
     1981 cctcgtcggg acccagtggg gggacgaggg caagggcaag gccaccgacc tcctcgccga
     2041 ccaggtcagc cacgtcgtcc gctaccaggg cggcaacaac gccggccaca cgatcatcgt
     2101 gggcgacgtc acgctgaagc tgcacctggt gcccagcggg gtcatgtacc cgcacgtcac
     2161 gccggtgatc ggcgacggcg tggtcctgga ccccggtgtg ctgctcgacg agctcgacgc
     2221 gctggtcgcg cagggcctgg acgtgtccaa gctcaagatc agcggcaacg cgcacctgat
     2281 catgccgtac caccgcgagc tggacgcggt cactgagcgc tacctgggcc gccagaagct
     2341 cggcaccacc aagcgcggca tcggcccggc ctacgcggac aaggcggcgc gcgtcggcct
     2401 gcgcgtgcag gacctgtacg acatgaagat cttccggcag aagctggaca cggtgctcaa
     2461 ggagcgcaac gcggttctgt cgcgggtcta caac
//
LOCUS       DATMKC010000338         3212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_170521, whole genome shotgun sequence.
ACCESSION   DATMKC010000338 DATMKC010000000
VERSION     DATMKC010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170521"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1095
                     /gene="pdxS"
                     /locus_tag="VNU01_09965"
     CDS             181..1095
                     /gene="pdxS"
                     /locus_tag="VNU01_09965"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWH32983.1"
                     /translation="MNETDTTPAAGAQTGTGRVKRGLADMLKGGVIMDVVTAEQARIA
                     EEAGAVAVMALERVPADIRRDGGVARMSDPDMIEAIMKEVSIPVMAKVRIGHFVEAQI
                     LQAIGVDYIDESEVLTPADEAHHIDKHRFTVPFVCGATNLGEALRRIGEGAAMVRSKG
                     EAGTGNVVEAVRHMRDITAGIRRLAALSSDELYAEAKELRAPVELVREVAQTGKLPVV
                     LFTAGGIATPADAALMMQLGADGVFVGSGIFKSGDPAKRARAIVEATTYSGDPDILAK
                     VSRDLGEAMVGINLDDLDASQRLAGRGW"
     gene            complement(1096..1515)
                     /locus_tag="VNU01_09970"
     CDS             complement(1096..1515)
                     /locus_tag="VNU01_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HWH32984.1"
                     /translation="MDGTTLNQKFAYAFGAVYVLVGLAGFAVTGGVGFASPDGNPLIL
                     FEVNPLHNIVHLAIGAAFLLGAKAGPMAARSTNMAIGAVYLLVGVVGLFIDRGAEYNL
                     LALNMADNWLHLVSGAAALGVATYAGKEMSASRRTAM"
     gene            1695..2291
                     /gene="pdxT"
                     /locus_tag="VNU01_09975"
     CDS             1695..2291
                     /gene="pdxT"
                     /locus_tag="VNU01_09975"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF013350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWH32985.1"
                     /translation="MGVLALQGDVLEHLSMLASAGARAVPVKRSEQLDDLDGLLIPGG
                     ESTTIGKLAVLYGLLEPMRKRIADGLPVFGTCAGAILLSRRALSAAGEPSEQPLLEAM
                     DTQVRRNAFGRQVRSFEADLDIDGVDGGPMHAVFIRAPWFEECGRDVEILAAAATPAG
                     AKVVVARQGALLASAFHPELAGDDRLHRLFVQMVRKER"
     gene            2295..3044
                     /locus_tag="VNU01_09980"
     CDS             2295..3044
                     /locus_tag="VNU01_09980"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWH32986.1"
                     /translation="MSGHSKWATIKRKKGALDAQRGKLFARLIRGIEAAAKVGGADPA
                     GNPTLQTAIDKAKAASVPKDNIERALKRASGAEEGVNYDTIYYEGYAPGGVALYVECL
                     TDNRNRAANDVRAAFNKHGGSLAEPGAVNYLFSRTGVILVPAAATTEDDLLMAGLEAG
                     ITDVTLDGDTFTVKCEPSDLMAVKAALEEAGIALTSADTTLLPSVTVQVTDETVAKQV
                     LRLLDALDDCDDVQEVYANFDIPDQVLEAVA"
BASE COUNT          476 a         1100 c         1180 g          456 t
ORIGIN      
        1 ggcgcaagga gtcgcgcgac agccacttcg agttcgtgac cgccgcgggc gtggtcgtcc
       61 cgctggcgcc ggggcgcacg tgggttgagc tgctgcccgc cggcacggcg gtcgacctgg
      121 tccgcccgca gcccgcgggc accgcgtcgc cgtcgtcctg acggcggtag gctggccgac
      181 gtgaacgaga ccgacaccac ccccgccgcc ggcgcgcaga ccggcaccgg acgcgtcaag
      241 cgcggcctgg ccgacatgct caagggcggc gtcatcatgg acgtcgtcac cgccgagcag
      301 gcgcgcatcg ccgaggaggc cggcgccgtc gccgtcatgg cgctggagcg cgtgcccgcc
      361 gacatccgcc gcgacggcgg tgtcgcccgc atgtccgacc ccgacatgat cgaagcgatc
      421 atgaaggagg tctccatccc ggtgatggcc aaggtgcgca tcggccactt cgtggaggcg
      481 cagatcctgc aggccatcgg cgtggactac atcgacgagt ccgaggtgct cacgcccgcc
      541 gacgaggcgc accacatcga caagcaccgc ttcacggtgc cgttcgtgtg cggggcgacc
      601 aacctgggcg aggcgctgcg gcgcatcggc gagggcgcgg cgatggtgcg ctccaagggc
      661 gaggccggca ccggcaacgt ggtggaggcc gtgcgccaca tgcgcgacat caccgcgggc
      721 atccgccgcc tcgccgcgct gagctccgac gagctgtacg cggaggccaa ggagctgcgc
      781 gcgccggtgg agctggtgcg cgaggtggcg cagaccggga agctgccggt ggtgctgttc
      841 accgccgggg gcatcgcgac gcccgccgac gccgcgctga tgatgcagct cggcgccgac
      901 ggggtgttcg tgggctcggg catcttcaag tccggcgacc cggccaagcg cgcgcgggcc
      961 attgtcgagg cgacgaccta ctcgggcgac ccggacatcc tcgccaaggt ctccagggac
     1021 ctcggcgagg cgatggtcgg gatcaacctc gacgacctgg acgcgtcgca gcgcctggcc
     1081 ggccgcggct ggtagctaca tcgcggtccg gcggctggcg ctcatctcct taccggcgta
     1141 cgtcgcgacg ccgagcgccg cggcgccgct gaccaggtgc agccagttgt cggccatgtt
     1201 cagcgccagg aggttgtact cggcgccgcg gtcgatgaac aggccgacca cgccgacgag
     1261 cagatacacc gcgccgatcg ccatgttggt ggagcgggcc gccatggggc cggccttggc
     1321 gcccagcagg aacgccgcgc cgatggccag gtgcacgatg ttgtgcagcg ggttgacctc
     1381 gaacaggatc agcgggttgc cgtccgggga agcgaagccg acgcctccgg tcaccgcgaa
     1441 gccggccagg cccacgagca cgtagaccgc gccgaaggcg tacgcgaact tctgattgag
     1501 cgtcgtgcca tccatgttcc caaccccttg tcgctctttg tggctcggtc cgcccggcct
     1561 acccgggcgg ggtgttcctg actcgcacgc aataggctgg ctccatgacc gagactcggc
     1621 gtgagcgggt tcccggcgag ggtctgatac ccacgcgggg gcctcgccat gtggtcggcg
     1681 accacggtcc cctggtgggc gtgctggccc tccagggcga cgtgctggag catctgtcca
     1741 tgctggcctc cgcgggcgcg cgtgccgttc cggtgaagcg ttcggagcag ctggacgact
     1801 tggatgggtt gctcatcccc ggcggggagt ccacgaccat cggcaagctg gcggtgctgt
     1861 acggcctgct ggagccgatg cgcaagcgca tcgccgacgg gctgcccgtg ttcggcacct
     1921 gcgcgggcgc gatcctgctg tcgcggcgcg ccctgtccgc ggcgggcgag ccgtccgagc
     1981 agccgctgct ggaggcgatg gacacgcagg tgcgccgcaa cgcgttcggc cgccaggtgc
     2041 gcagcttcga ggcggatctg gacatcgacg gggtggacgg cgggccgatg cacgcggtgt
     2101 tcatccgcgc gccgtggttc gaggagtgcg ggcgggacgt ggaaatcctc gccgcagccg
     2161 ccactccggc cggtgctaaa gtcgtcgtgg cccgccaggg ggcgctcctg gcgtcggcct
     2221 tccaccctga gctggcgggc gacgaccgcc tgcaccggtt gttcgtccag atggtgcgca
     2281 aggagaggta agccgtgtcc ggtcattcca agtgggcgac catcaagcgc aagaagggcg
     2341 cgctggacgc gcagcgcggc aagctcttcg cgcgcctgat ccggggcatc gaggcggcgg
     2401 ccaaggtggg cggcgcggac ccggccggca accccacgct gcagaccgcg atcgacaagg
     2461 ccaaggccgc ctccgtcccc aaggacaaca tcgagcgtgc gctgaagcgc gcgtccggcg
     2521 ccgaggaggg cgtcaactac gacaccatct actacgaggg ctacgcgccc ggcggtgtcg
     2581 ccctgtacgt ggagtgcctg accgacaacc gcaaccgggc ggccaacgac gtgcgcgcgg
     2641 cgttcaacaa gcacggcggc tcgctggccg agcccggtgc ggtcaactac ctgttctcgc
     2701 gcacgggcgt gatcctggtg cccgcggccg cgaccaccga ggacgacctg ctgatggcgg
     2761 gcctggaggc cggcatcacc gacgtcacgc tggacgggga cacgttcacg gtgaagtgcg
     2821 agccgtccga cctcatggcc gtgaaggcgg cgctggagga ggcgggcatc gcgctgacgt
     2881 cggcggacac cacgctgctg ccgtcggtga cggtgcaggt gaccgacgag accgtggcca
     2941 agcaggtgct gcgcctgctg gacgcgctgg acgactgcga cgacgtccag gaggtctacg
     3001 cgaacttcga catccccgac caggtcctcg aagccgtcgc ctgacccgca cctgttggca
     3061 acgcgacatc gggtcggatt cggtgcgcat gtgcacctga tccgacccat tgtttcgatg
     3121 tccacagtcg ggcggctcgt tttcccgccg tggccgtgcc gctgacgcaa gctgcggctc
     3181 atggacaagt ggggacagct cgcttgggag gg
//
LOCUS       DATMKC010000339         5454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_198211, whole genome shotgun sequence.
ACCESSION   DATMKC010000339 DATMKC010000000
VERSION     DATMKC010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198211"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VNU01_09985"
     CDS             <1..334
                     /locus_tag="VNU01_09985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWH32987.1"
                     /translation="ASGAHGDDEANLRLVLARMEGVADRAARFVCVAALALPDGRERT
                     VRGELEGMLTEAPRGSGGFGYDPIFLPLGERRTTAEMPPEDKDAISHRGKAFRALRPA
                     VAELLGLG"
     gene            complement(354..557)
                     /locus_tag="VNU01_09990"
     CDS             complement(354..557)
                     /locus_tag="VNU01_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32988.1"
                     /translation="MSDTTDIIGSHCDHGACSAPVRVHVRLAGGELGFCMHHWVALEE
                     AMVATPSFVAASPVDRVLSAAGA"
     gene            686..1726
                     /locus_tag="VNU01_09995"
     CDS             686..1726
                     /locus_tag="VNU01_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWH32989.1"
                     /translation="MTATHPAPTLAPRPVSPAGPPLPQPRGPLSAYLLDRLRLPVHAL
                     PAPPAAEDDPLWGEDSALALYCCYELHYRGLAGVDDAWEWEPSLLALRRALEDAFEVR
                     LREVVGPVEAPGDVAAALEAVAHPADDGGRSLADFAADEATDSQFKELAVHRTAWQLK
                     EADPHTWAIPRLWGKPKAALVEIQKEEYGEGVEKDIHAVLYAMTLRELGLDDRYGAYV
                     DHLPAVTLATVSLASFFGLHRRRRGAAVGHLSMFEMTSVEPMGKYSVGLRRLGYSSWA
                     RLFYDTHVVADANHQFVAAHKLAAGLAQQEPEVAGDIVFGAKALMALEAELTEHAFAC
                     FDAGRTSLRRPL"
     gene            1796..3139
                     /locus_tag="VNU01_10000"
     CDS             1796..3139
                     /locus_tag="VNU01_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH32990.1"
                     /translation="MPDVDVIVVGAGLAGLRAADLLRARGLTTLVLEARDRVGGRTWT
                     TELAGAPVELGAQWVGPGQPRILALLDELGLATHHVAHDGRKVVAAGGRVRTYSGTVP
                     PLSPTALAGLQGALWAMDRLARTVDPAAPWDARRAAELDGRTLEQWKRRHIPPGSGRS
                     VFDAAIRVVFGAEPGELSLLWAAHYVAGGGGMEALVEVQGGAQERRISAGTQALALAL
                     SARLGDAVRLSHPVERVVQDAGGVTVTGPWGQARAGRLVVALPPHLAGRIAFAPLLPV
                     ARDALTQRFPMGQTIKVQVRYERAFWRARGLSGEAVLADGPVSVVIDGSGAEGAAPGL
                     SAFVVAEPGRQASRWTPETRRERVLDQLARCFGDEAREPLGYADLDWAAQEWSRGCPT
                     GSAVPGALSRLGPALREACGRVHWAGTETARQWAGYMEGALESAERVAAEVGAAR"
     gene            3151..3555
                     /locus_tag="VNU01_10005"
     CDS             3151..3555
                     /locus_tag="VNU01_10005"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HWH32991.1"
                     /translation="MRTDINPGREAFAELLARADEGPVVMLNLLKFRDDAGDGETGRQ
                     AYNRYAQAIGPMIEELGGRTRWIGDTGTLLVGDPQAQDWDAILLVEYPTRRAFADMVS
                     RPDYLAAHEHRERALERTLLLQTHAVPGGAPF"
     gene            complement(3622..>5454)
                     /locus_tag="VNU01_10010"
     CDS             complement(3622..>5454)
                     /locus_tag="VNU01_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH32992.1"
                     /translation="TGKRVNIPITGTPRNVAGKPIDPAEQNRNDGFSPGALIVSVVPG
                     VDLARTGAPDLLGGFDDSLAPDSPIVLLNADTGERHPFWAELDEWDQYEPGQERPLLI
                     RPAVNFTEGHRYIVALRDMKDAAGGTIQPNEAFAKILEPGVPGRSQGRDRHFEDEIFP
                     RLAAAGVGRDGMYLAWDFTVASAENLAGRVLHMRDDAFATLGDAVPAYEVTGTFDNPS
                     SQIARRVEGAVKVPNYLTLPGGAPGSNIRYGADGKPEQFPGGGTMDAKFWCNIPASAS
                     PSNPSRISLYGHGLLGSGSQVSNGYVRDYANRDNITFCGVDWIGMSTEDLPVIGAILA
                     DMSLFSKLPDRAQQGFVNFMFVGRALKHPQGFVTDPAFQPTGEPLLDTGELFYDGNSQ
                     GGIMGGALMALTQDVTRGVLGVPGQNYSTLLDRSVDWEPYYAVFDAAYPDRLEQQIVF
                     SMIQMLWDRGEANGYAAHITHDPYPGTPSHQVLLQAAYGDHQVTIVAAEVMARTIGAY
                     AHQPQVAKHYDAPELWGLEQIPYGSDTGAISAMTLWDHGNDPIPLGNVPPTTDRYDPH
                     GLTRREPRAMDQKSAFMRTGGSVINVCDVPVVGGPCIATERRVD"
BASE COUNT          673 a         1923 c         2109 g          749 t
ORIGIN      
        1 agcgagcgga gcgcacggcg acgacgaggc gaacctgcgc ctggtgctgg cgcgcatgga
       61 gggcgtggcc gaccgggcgg cgcggttcgt ctgcgtcgcg gcgctggcgc tgcccgacgg
      121 gcgcgagcgc accgtgcgcg gcgagctgga gggcatgctc accgaggcgc cccgcggcag
      181 cggcggcttc ggctacgacc cgatcttcct gccgctgggc gagcggcgca ccaccgcgga
      241 gatgccgccg gaggacaagg acgcgatctc gcaccgcggc aaggcgttcc gcgccctgcg
      301 gcccgccgtg gccgagctgc tcggcctggg ctgaggcggc tgcggacgac ggctcaggcg
      361 ccggcggcgc tgagcacgcg gtccaccggc gaggcggcca cgaacgacgg cgtggcgacc
      421 atggcctctt ccagggcgac ccagtggtgc atgcagaagc ccagctcgcc gcccgccagg
      481 cggacgtgca cgcgcaccgg cgcgctgcac gcgccgtggt cgcagtgcga gccgatgatg
      541 tccgtggtgt cgctcatcct cccgctcccc ttcgaccagt cgtcgtgcgt cgtattcgtg
      601 tcaacgcgtg acggccgcgc gggatacggt gcgaatcggg cggcttgccg cttcggcgtc
      661 cgcacaaccg ggtaaatgcc gcgccatgac cgcgacgcat ccggcgccga ccctcgcgcc
      721 acgccccgtt tcccccgccg gaccgccgct gccgcagccg cgcgggccgc tgtccgccta
      781 tctgctggat cgcctgcgcc tgcccgtgca cgcgctgccg gcgccgccgg ccgcggagga
      841 cgacccgctg tggggcgagg acagcgcgct ggcgctgtac tgctgctacg agctgcacta
      901 ccgcggcctg gccggcgtcg acgacgcctg ggagtgggag ccgtcgctgc tcgcgctccg
      961 gcgtgccctg gaggatgcct tcgaggtgcg cctgcgcgag gtggtcgggc cggtggaggc
     1021 gcccggcgac gtggccgcgg ccctggaggc ggtcgcccac cccgccgacg acggcggccg
     1081 ctccctggcc gacttcgccg ccgacgaggc cacggacagc cagttcaagg agctcgccgt
     1141 gcaccgcacc gcgtggcagc tcaaggaggc cgacccgcac acctgggcga tcccgcggct
     1201 gtggggcaag cccaaggcgg cgctggtgga gatccagaag gaggagtacg gcgagggcgt
     1261 cgagaaggac atccacgcgg tgctgtacgc catgacgctg cgcgagctgg gcctggacga
     1321 ccgctacggc gcctacgtgg accacctgcc cgccgtgacg ctggcgaccg tcagcctggc
     1381 gagcttcttc ggcctgcacc ggcggcgccg cggcgccgcg gtcggccacc tgtcgatgtt
     1441 cgagatgacg tcggtggagc cgatgggcaa gtactcggtg ggcctgcggc gtctgggcta
     1501 cagctcgtgg gcgcggctgt tctacgacac gcacgtggtg gccgacgcga accatcagtt
     1561 cgtcgccgcg cacaagctgg ccgccgggct ggcgcagcag gagccggagg tggccggcga
     1621 catcgtgttc ggcgccaagg cgctcatggc gctggaggcc gagctcaccg agcacgcgtt
     1681 cgcctgcttc gacgcgggcc gcacgtccct gcgccgcccc ctctgacctc caaggcgggt
     1741 gcactccggc gcgtatgtgc gccggagtgc acccggctcg ggctagtttc tgtggatgcc
     1801 cgacgtcgac gtcatcgtgg tgggcgcagg cctggccggt ctgcgtgcgg ccgacctgtt
     1861 gcgcgcgcgg ggcttgacga ccctggtcct cgaggcgcgc gaccgcgtgg gcgggcgcac
     1921 ctggaccacc gagctcgccg gcgcgccggt ggaactgggc gcccagtggg tcgggccggg
     1981 acagccgcgc atcctggcgc tgctggacga gctgggcctg gccacccacc acgtcgccca
     2041 cgacggccgc aaggtggtcg ccgcgggcgg cagggtgcgc acgtactccg gcaccgtgcc
     2101 gccgctgtca ccgaccgcgc tggccggcct gcagggcgcg ctgtgggcga tggaccgcct
     2161 ggcgcgcacc gtcgaccccg cggcgccatg ggacgcccgg cgggccgcgg agctggacgg
     2221 ccgcacgctg gagcagtgga agcgccgcca catcccgccg ggctccggcc gaagtgtctt
     2281 cgacgccgcc atccgcgtcg tcttcggcgc cgagccgggc gagctgtcgc tgctgtgggc
     2341 ggcccactac gtcgccgggg gcggcggcat ggaagcactg gtggaggtcc agggcggcgc
     2401 gcaggagcgc cgcatctcgg ccggcacgca agcgctggcc ctggcgctgt ccgcccggct
     2461 gggcgacgcc gtacggctga gccatcccgt ggaacgcgtg gtgcaggacg ccggcggcgt
     2521 gacggtgacc ggcccgtggg gccaggcccg tgccggccgg ctcgtcgtgg cgctcccgcc
     2581 gcacctggcc gggcgcatcg ccttcgcgcc gctgctgccg gtggcgcgag acgcgctgac
     2641 ccagcgcttc cccatgggtc agaccatcaa ggtgcaggtg cgctacgagc gggcgttctg
     2701 gcgcgcccgc ggcctgtcgg gtgaggcggt gctggccgac ggcccggtca gcgtggtgat
     2761 cgacggctcc ggcgccgagg gggccgcgcc gggcctgtcg gcgttcgtcg tggccgagcc
     2821 gggccgccag gcgtcgcggt ggacgccaga gacgcggcgg gagcgggtcc tggaccagct
     2881 cgcgcgctgc ttcggggacg aggcgcgtga gccgctcggc tacgccgacc tggactgggc
     2941 ggcgcaggag tggtcgcgcg gctgtcccac cggctcggcc gtgcccggtg cgctcagccg
     3001 gctggggccg gcgctgcgcg aggcgtgcgg gcgcgtgcac tgggccggca cggagaccgc
     3061 gcgccagtgg gccggctaca tggagggggc gctggagtcg gcggagcgcg tggccgccga
     3121 ggtgggcgcc gcgcggtagc gtcccgggcc atgagaaccg acatcaaccc gggacgggag
     3181 gcgttcgccg agctgctcgc gcgcgccgac gaaggccccg tcgtcatgct gaacctgctc
     3241 aagttccgcg acgacgcggg cgacggcgag accggccggc aggcctacaa ccgctacgcg
     3301 caggcgatcg ggcccatgat cgaggagctg ggcgggcgta cgcgctggat cggcgacacc
     3361 ggcacgctgc tggtcggcga cccccaggcg caggactggg acgcgatcct gctggtggag
     3421 tacccgacgc gccgggcgtt cgccgacatg gtcagccgcc cggactacct ggccgcgcac
     3481 gagcaccgcg agcgggcgct ggagcgcacc ctgctgctgc agacccacgc cgtccctggc
     3541 ggcgcgccct tctaggaacg ccgaacgggt gcgggtcccg cgagatgtcg cggtgcccgc
     3601 acccgttcga atggggacgc gtcagtcgac gcggcgctcc gtggcgatgc acgggccacc
     3661 cacgaccggc acgtcgcaga cgttgatcac cgagccgccg gtgcgcatga acgccgactt
     3721 ctgatccatg gcgcgcggct ccctgcgcgt caggccgtgc gggtcgtagc ggtccgtggt
     3781 cggcgggacg ttgcccagcg ggatcgggtc gttgccgtgg tcccacaggg tcatggcgct
     3841 gatcgcgccg gtgtccgagc cgtacgggat ctgctccagg ccccacagct cgggcgcgtc
     3901 gtagtgcttg gcgacctgcg gctggtgggc gtaggcgccg atcgtgcgcg ccatgacctc
     3961 ggccgccacg atcgtcacct ggtggtcgcc gtaggccgcc tgcagcagca cctggtgcga
     4021 cggggtgccc gggtacgggt cgtgggtgat gtgggcggcg tagccgttgg cctcgccgcg
     4081 gtcccacagc atctggatca tcgagaagac gatctgctgc tccagccggt ccgggtaggc
     4141 cgcgtcgaag acggcgtagt acggctccca gtccacactg cggtcgagca gggtggagta
     4201 gttctggccg ggcacgccca gcacgccgcg cgtgacgtcc tgcgtgagcg ccatgagcgc
     4261 gccgcccatg atgccgccct ggctgttgcc gtcgtagaac agctcgccgg tgtccagcag
     4321 cggctcaccg gtgggctgga acgcggggtc ggtgacgaag ccctgcgggt gcttgagcgc
     4381 gcggcccacg aacatgaagt tcacgaagcc ctgctgcgcg cggtcgggca gcttggagaa
     4441 cagcgacatg tcggccagga tcgcgccgat gaccggcagg tcctccgtcg acatgccgat
     4501 ccagtcgacg ccgcagaacg tgatgttgtc gcggttggcg tagtcgcgga cgtagccgtt
     4561 gctcacctgg cttccggagc ccagcaggcc gtgcccgtac aggctgatgc ggctggggtt
     4621 ggacggcgag gccgacgcgg ggatgttgca ccagaacttc gcgtccatgg tgccgccacc
     4681 cgggaactgc tccggcttgc cgtccgcccc gtagcggatg ttggagccgg gcgcgccgcc
     4741 cggcagcgtc aggtagttgg gcaccttgac ggcgccctcg acgcgccggg cgatctggga
     4801 cgacgggttg tcgaacgtgc cggtcacctc gtaggccggg acggcgtcgc ccagcgtggc
     4861 gaacgcgtcg tcgcgcatgt gcagcacgcg gccggcgagg ttctcggcgc tggcgacggt
     4921 gaagtcccac gccaggtaca tgccgtcgcg gccgacgccg gccgcggcca gccgcgggaa
     4981 gatctcgtcc tcgaagtggc ggtcgcggcc ctggctgcgg ccgggcacgc ccggctccag
     5041 gatcttggcg aacgcctcgt tcggctggat cgtgccgccg gcggcgtcct tcatgtcgcg
     5101 cagcgccacg atgtagcgat gcccttccgt gaagttcacc gcgggacgga tcagcagcgg
     5161 gcgctcctgg ccgggctcgt actggtccca ctcgtccagc tccgcccaga acgggtggcg
     5221 ctcgcccgtg tcggcgttca gcaggacgat cggggagtcc ggggcgagcg agtcgtcgaa
     5281 gccgcccagc aggtccggtg cgccggtgcg cgccaggtcc acgccgggca ccacggagac
     5341 gatcagcgcg ccgggggaga agccgtcgtt gcggttctgc tcggccgggt cgatcggctt
     5401 gccggcgacg ttgcgcggcg tgcccgtgat cgggatgttc acgcgcttgc cggt
//
LOCUS       DATMKC010000340         4922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_33368, whole genome shotgun sequence.
ACCESSION   DATMKC010000340 DATMKC010000000
VERSION     DATMKC010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33368"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2414)
                     /locus_tag="VNU01_10015"
     CDS             complement(<1..2414)
                     /locus_tag="VNU01_10015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH32993.1"
                     /translation="MSAAVVAAFGPDAHTLRVVSALRAQLPGTPIVVYAGNRAGLDAL
                     ASLDQGADVVHAAGANGLREALSRCEPGPVLLTHDDVVFGPDMAARLLRAHAPGRVVV
                     PYLNAPGTGHDLGALRPGQDVAAVLARAAEKRPGKGKLHKIQPSVVLADRDLLFTQGA
                     FRHIAAPGAVLGTLEMDFHAARGAVAAHVAACRRREVPAERLDGRPLLVATLIVRDEE
                     HMLPDCLASLRAVADEIVVCDTGSTDRTVEIAEAAGARVIHREWRNDFGWARNEVIEA
                     AGNPWYVIVIDADERLECDDPAALRTVLAQGIDEHRVYEVQVANSAISRSVSGVFMGS
                     RVFRADEVRYVGALHEQPTPLAELPPVLRATLAGLRVLHLGYDPTMMVSRDKAERNLA
                     IAREGYETERTPHAALHYARSLAGADHDRGLQRRLLAEVLEAGPSAPIESYALTIMAE
                     YDLDEGDAEAALARARDAVQRTPADDLASLVFAKAAETLGRIDEIAELARWRRNRPSA
                     EPAHTMLDLSAEAYGREAAARAQAGDLAGAVADASAAIEMYGLPPEAWRRVATAVLDR
                     RPTQVAETLGPLVARDAGHAIPALSTVLPPARHHTTPVAERLGRVVLFLEEENGDERT
                     RAAVERYAAAAAGEGCAVVRHVTTERSPYARASAGGERLPLADASGLVEALRPEVVVF
                     ASMVRFNRDSRCLVFNPESRVMLLVPASPYAPGDTIDAVVHPEDDEGEKRLLDLLPSP
                     KASAPPRIRTIERVEPTAGLTSVVIMGTGDQELLEQCVAALDAHTTEPFELVLVGEGS
                     GAVESGR"
     gene            2907..4100
                     /locus_tag="VNU01_10020"
     CDS             2907..4100
                     /locus_tag="VNU01_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWH32994.1"
                     /translation="MRINQNIMAFNSYRNLTTTNTQLGKSLEKLSSGFRINRAADDAA
                     GLVVSEGLRAQISGLRVATRNAQDGVSVVQTAEGALTEVHSMLRRMRDLAVQASNTGA
                     NDADARAAAQAEIDELTAEVGRISDKTKFGGTALLDGTYGISPATVKSDTAGFAATGG
                     SFAFTVNDQFTVRVDGGAAVTVTLDASTRTADGLETYLQNKIQGALYADNQDGSKVTV
                     KVTKDDTDTWRVSIEAAVASGAALTLANQSGTPLTAQLGINAGTLADTASGSGGVFQV
                     GANAGETIAVSIDDMDATALGINALDVETDASGAITAIDAAIKTVSTQRGELGAVQNR
                     FEHTVANLSVTAENLAASESRIRDTDMALELVDFTRHQILQQAGTAMLAQANQVPSSV
                     LSLLG"
     gene            4227..>4922
                     /locus_tag="VNU01_10025"
     CDS             4227..>4922
                     /locus_tag="VNU01_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HWH32995.1"
                     /translation="MSGTFSVGGLASGLDTQNIIAQLMQLERVPIQRLTLRQAKLNRV
                     DDAWKTVVSKLSAVRNSTNNLRTQLEFEKFSKATSSNSDVIAASVHGTPSLGAVDVTV
                     VALAYAEQRASNENFASADAAMGTRELDITTSSGTYTITPDSADMTLADFVGKINKTV
                     PEVRAQAVQIADGEYELVISAKQTGTANDFSVVATNWTGAWTQTQAAADAQVRMGDPA
                     TGVLVTRSSNTITD"
BASE COUNT          758 a         1761 c         1709 g          694 t
ORIGIN      
        1 cgtccggact ccacggcacc gctcccttcg cccacgagca ccagttcgaa gggttccgtc
       61 gtgtgcgcgt cgagcgcggc cacgcactgt tccagcagct cctggtcgcc ggttcccatg
      121 atcaccacgc tggtgaggcc ggccgtcggc tccacgcgct cgatcgtgcg gatgcgcggc
      181 ggcgccgagg ccttcgggct ggggagcagg tccaggaggc gcttctcgcc ctcgtcgtcc
      241 tcggggtgca ccaccgcgtc gatggtgtcg cccggcgcat agggagacgc cgggaccagc
      301 aacatgacgc gggactcggg gttgaacacc agacaccgcg agtcgcggtt gaagcgcacc
      361 atggaggcga agacgacgac ctcgggacgc agtgcctcga cgagcccgct ggcgtcggcg
      421 agcggcagtc gttcgccccc ggcgctcgcc cgcgcgtagg ggctgcgctc cgtcgtgacg
      481 tgtcgcacca cggcacaacc ctcgccggct gccgcggcgg catagcgctc gacggccgca
      541 cgcgtgcgct cgtccccgtt ctcctcctcc aggaacaaca cgacccggcc cagccgctcc
      601 gcgaccgggg tggtgtggtg gcgcgcgggc ggcagcacgg tcgacagcgc ggggatggcg
      661 tgtccggcgt ccctggccac cagggggccg agcgtctccg ccacctgggt cggtcgccgg
      721 tccagcacgg ccgtggccac ccgtcgccag gcctccggcg gcagtccgta catttcgatc
      781 gccgcggacg cgtccgccac cgcgcccgca aggtccccgg cctgggcccg ggcggcggcc
      841 tcgcgcccgt aggcttccgc cgacaggtcc agcatggtgt gcgccggctc ggccgacgga
      901 cgattgcggc gccaccgcgc cagctcggcg atctcgtcga tgcgccccag cgtctccgcc
      961 gccttcgcga agaccagcga ggccaggtcg tcggcgggcg ttcgctggac cgcgtcccgg
     1021 gcgcgggcca gcgccgcctc cgcatcgccc tcgtccaggt cgtactccgc catgatggtg
     1081 agtgcgtagg actcgatcgg cgccgatgga cccgcttcca gcacttcggc gagcagcctg
     1141 cgctgcagcc cgcgatcgtg gtccgcgccc gcgagactgc gcgcgtagtg cagcgcggcg
     1201 tgcggggtcc tttcggtctc gtagccttcc cgcgcgatcg ccaggttgcg ctccgccttg
     1261 tcgcggctga ccatcatcgt cgggtcgtag cccaggtgca gcacccggag tccggccagc
     1321 gtggcgcgca gcaccggcgg cagttccgcc aagggcgtgg gctgctcgtg cagcgcgccg
     1381 acataccgga cctcgtcggc ccggaacacc cgactgccca tgaacacgcc gctgaccgag
     1441 cggctgatcg cggaattggc gacctggacc tcgtacacgc ggtgctcgtc gatcccctgg
     1501 gccagcacgg tgcgcagggc ggcggggtcg tcgcactcca gccgctcgtc cgcgtcgatg
     1561 acgatgacgt accaggggtt cccggcggcc tcgatgacct cgttgcgcgc ccagccgaag
     1621 tcgttgcgcc attcgcggtg gatcacccgc gcgcccgccg cctcggcgat ctcgacggtc
     1681 cggtccgtgg acccggtgtc gcacaccacg atctcgtcgg cgaccgcgcg cagggacgcc
     1741 aggcagtccg gcagcatgtg ctcctcgtcc ctgacgatga gcgtcgccac caagagcggc
     1801 cggccgtcca gccgctccgc cggcacctcg cggcgccggc acgccgcgac gtgcgccgcg
     1861 accgccccgc gcgcggcgtg gaagtccatc tccagcgtgc cgagcaccgc gcccggggcg
     1921 gcgatgtgcc ggaaggcgcc ctgggtgaac aacaggtcgc ggtccgccag cacgaccgac
     1981 ggctggatct tgtgcagctt gcccttgccg ggacgcttct ccgcggcccg cgcgagcacg
     2041 gcggcgacat cctggccggg acgcagcgca cccaggtcgt gcccggtgcc gggggcgttg
     2101 aggtagggga ccaccacgcg gcccggcgcg tgcgcgcgca gcagccgtgc cgccatgtcc
     2161 ggtccgaaca cgacgtcgtc gtgcgtgagc agcacggggc ctggctcaca ccgggagagc
     2221 gcctcgcgga gcccgttcgc ccccgcggcg tggacgacgt cggcgccctg atccagggaa
     2281 gccagcgcgt cgagtccggc gcggtttccc gcgtagacga cgatgggcgt gccgggcagc
     2341 tgggcgcgca gcgccgacac gacccgcaac gtgtgggcgt cgggcccgaa cgccgcgacg
     2401 accgcggcgg acattctgct cacgtgcaag cccccggatg ttcgaggcga ctgggtcagg
     2461 ttccccgtcg gccgcagtgc ggagtttctc aaggctgttt caggatcggg attcaagcat
     2521 gacgcccgac gcgccgaggc cttctgggtg cgtgtcgccc gaacgtgcac tgcccaggag
     2581 tacaggttgc tgagcgtgat atcaccccta ccccccgcgc ttccgttcgt gtagtttcat
     2641 cgagctggtc ccgcgtgagc gggacgagtg cgccgtgtcg gaccgtccga ggcggcgctg
     2701 ctggggtcgg gccggcgcat gggatgtccc tcgccgggga gaccgggagt cgtcaaggag
     2761 gaaagtccgc gaagttctcg gtcagacgtt aagtgaccct cccctgggcc gacagggaga
     2821 cgtgaaccag aggcttttcc gcttcaggtt tgcggccacg gaacggccgc caggcgatat
     2881 gggtgacaac agggaggttc aatcccatgc gcatcaacca gaacatcatg gcgttcaatt
     2941 cctatcggaa tttgacgacc accaacacac agctgggcaa gtccctcgag aagctgtcga
     3001 gtggcttccg gatcaaccgg gcggcggacg acgccgccgg tcttgtcgtg tccgagggtc
     3061 tgcgtgcgca gatcagcggt ctgcgcgtgg ccacccgcaa cgcgcaggac ggcgtctcgg
     3121 tggtgcagac cgccgagggt gccctcaccg aggtgcactc catgcttcgt cgtatgcgtg
     3181 acctggccgt gcaggccagc aacacgggtg cgaacgacgc cgacgctcgt gcggccgccc
     3241 aggccgagat cgacgagctg accgccgagg tcggccgcat ctctgacaag accaagttcg
     3301 gcgggaccgc cctgctggac ggcacgtacg gcatttcgcc cgcgaccgtc aagtcggaca
     3361 ccgccgggtt cgccgccacc ggcggcagct tcgccttcac cgtcaacgac cagttcaccg
     3421 tccgcgtgga cggtggcgca gccgtgacgg tgaccctgga cgcctcgacg cgcaccgcgg
     3481 acggtctgga gacctacctc cagaacaaga tccagggtgc gctgtacgcg gacaaccagg
     3541 acggcagcaa ggtcaccgtc aaggtgacca aggacgacac cgacacctgg cgcgtctcga
     3601 tcgaggctgc cgtggccagc ggtgccgccc tcacgctggc gaaccagagc ggcacgccgc
     3661 tgacggccca actcggcatc aacgcgggca cgctggcgga caccgcttcc ggttccggtg
     3721 gtgtgttcca ggtcggcgcc aacgccggcg agaccatcgc ggtgtccatc gacgacatgg
     3781 acgccacggc gctgggcatc aacgccctgg acgtcgagac ggacgcgagc ggcgcgatca
     3841 cggcgatcga cgccgcgatc aagaccgtgt ccacgcagcg cggtgagctg ggtgccgtgc
     3901 agaaccggtt cgagcacacg gtcgccaacc tctcggtcac ggccgagaac ctggcggcgt
     3961 ccgagagccg gatccgcgac accgacatgg ccctggaact ggtggacttc acgcgtcacc
     4021 agatcctgca gcaggccggc acggcgatgt tggcccaggc caaccaggtg ccgagctcgg
     4081 tcctgtcgct gctcggctag gtccttccgg gctgaagccg ggccaacccg taccgactgg
     4141 tccggtggcg tcgccgcaag atggcggcgc caccggccgg cggcccggtc gacagaggtg
     4201 accagcagag aggaggcggg gagcgcatga gcgggacgtt cagcgtcggc ggcctcgcgt
     4261 ccggcctcga cacccagaac atcatcgccc agctcatgca gcttgagcgc gtgccgatcc
     4321 agcgcctcac ccttcggcag gcgaagctca accgcgtcga cgacgcgtgg aaaaccgtcg
     4381 tgtcgaagct ttccgcggtg cgcaactcca cgaacaacct gcgcacccag ctcgagttcg
     4441 agaagttctc gaaggcgacg tcgtcgaact cggacgtgat cgccgcttcg gtgcacggga
     4501 caccgtcact cggcgccgtc gatgtgacgg tggtggccct ggcctacgcc gagcagcgtg
     4561 ccagcaacga aaatttcgcg agcgcggacg cggcgatggg cacgcgtgag ctggacatca
     4621 cgacctccag cggcacctac accatcacgc ccgacagcgc cgacatgacg ctggcggact
     4681 tcgtcggcaa gatcaacaag accgtccccg aagtgcgtgc gcaggcggtg cagatcgccg
     4741 acggcgagta cgagctcgtg atcagcgcga agcagaccgg cacggccaac gacttcagcg
     4801 tcgtggcgac gaactggaca ggcgcgtgga cgcagacgca ggcggctgcc gacgcccagg
     4861 tgcggatggg cgaccccgcc actggcgtgc tggtcacccg ctcgtcgaac acgatcaccg
     4921 ac
//
LOCUS       DATMKC010000341         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_198233, whole genome shotgun sequence.
ACCESSION   DATMKC010000341 DATMKC010000000
VERSION     DATMKC010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198233"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VNU01_10030"
     CDS             <1..276
                     /locus_tag="VNU01_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HWH32996.1"
                     /translation="RLYFWPEPHGHGSLRPTLSDAAWPDSRSCAPSDGSGSYSVAASP
                     AAVGSAKAAAELGAHERLSGQAASDKVGRNDPCPCGSGQKYKRCHGA"
     gene            complement(280..1221)
                     /locus_tag="VNU01_10035"
     CDS             complement(280..1221)
                     /locus_tag="VNU01_10035"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWH32997.1"
                     /translation="MRPLLLDVLREHADPEEPVCVPSPAVASVLIDDGWRVLAGRVED
                     QPSAALGGVVLVDDELGHSGEHAEGLLAQAARVLRPGGLLAVSARGRIHAEAIGVRPA
                     GRAFSAAELAHALGHRGFGVEVLAAPGAATGLRGVVPAYDPELDRHPGLLDSAEHLVA
                     LARWAPDEATRSERFFASLPRKVVAASVVCRDREERLLLVFDSYKRHWTLPGGVVDAD
                     EDPRAAAEREAWEEAGVRVRAGRILGVFSGSWPDRVVLVYEARLTEPAPTPVPVHHHE
                     VDAVSWMALDEAIRAVPPHVAFQITRSIRQPGGSWRQ"
     gene            complement(1226..2257)
                     /locus_tag="VNU01_10040"
     CDS             complement(1226..2257)
                     /locus_tag="VNU01_10040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH32998.1"
                     /translation="MLVASLALSGLGVPAQAADARLEKAQQQRVQAQRELDKLVGRLS
                     TLEAEVAQVENRLAELAAAERRAQEQAAGASAALRLRVRESYKRGTGDPMLALFTSES
                     PQQAAAQARLLGLLAKRSRSDFESATAARIRTQATALDVERVAADLRSRQARLDAVRA
                     QVAGLLAKAQQRERHVRDRIAKEQAAKRRVARRTTRQSGTRAAEGAAAVVNGAACPVG
                     NPRSYSDTWGAPRSGGRRHKGTDILAPRGTGIYAYESGSITRLTNSRVGGISIYMRGA
                     SGNTYYYTHLQGYVSGLSAGQAVNAGEHIAFNGDTGNAQGTPHLHFEVMPGGGGNVNP
                     YPYVKRACG"
     gene            complement(2316..2840)
                     /locus_tag="VNU01_10045"
     CDS             complement(2316..2840)
                     /locus_tag="VNU01_10045"
                     /inference="COORDINATES: protein motif:HMM:NF039460.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HWH32999.1"
                     /translation="MTLESEADPLILALAFAQLYLEVECGRRPRATVEPLMTPRLAGR
                     LGDGWVRGGPMRRPGAARGSLAAPDAFEAAVTAHGPQRTGVLALRLERRAAGWRVTEV
                     VRPEDGALPEAEPPPLERIEVESPDRWSDEPGGPDITVIAAPWGLPAGVAAEVCAPGG
                     PSRCWSSRSGTATL"
     gene            complement(2837..>3145)
                     /locus_tag="VNU01_10050"
     CDS             complement(2837..>3145)
                     /locus_tag="VNU01_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33000.1"
                     /translation="PQGAAGPMGDWLRDGAAAWPAGPPPYPPAAPWVAPPEQDDGRPR
                     WSWGTAAPWVVAGLLCALLLTSRAAPPAPEPVAVPSPAAPAELAAVPAPQPACVEVAQ
                     "
BASE COUNT          371 a         1229 c         1179 g          366 t
ORIGIN      
        1 cgcttgtact tctggcccga gccgcacggg cacgggtcgt tgcgccccac cttgtccgac
       61 gccgcctggc cggacagccg ctcgtgcgcg cccagcgacg gctcgggctc gtactcggtg
      121 gcggcgagcc cggccgcggt cggctcggcc aaggccgccg cggagctggg cgcgcacgag
      181 cggctgtccg gccaggcggc gtcggacaag gtggggcgca acgacccgtg cccgtgcggc
      241 tcgggccaga agtacaagcg ctgccacggc gcctgacggt tactggcgcc aggagccgcc
      301 gggctggcgg atgctgcggg tgatctggaa cgcgacgtgc gggggcacgg cgcggatcgc
      361 ctcgtccagc gccatccagc tgaccgcgtc gacctcgtgg tggtgcaccg gcaccggtgt
      421 gggcgcgggc tcggtgagcc gcgcctcgta gaccagcacc acccggtcgg gccaggagcc
      481 ggagaacacg cccaggatcc tgccggcccg cacgcgcacg ccggcttcct cccaggcctc
      541 gcgctccgcg gcggcgcgcg ggtcctcgtc ggcgtccacc acgccgccgg gcagcgtcca
      601 gtggcgcttg tagctgtcga acaccagcag cagccgctcc tcccggtccc ggcacaccac
      661 cgacgcggcg accaccttgc gggggaggct ggcgaagaag cgctcgctgc gcgtggcctc
      721 gtccggcgcc cagcgcgcca gcgccaccag atgctcggcg ctgtcgagca gccccgggtg
      781 gcggtccagt tccgggtcgt aggcgggcac gacaccgcgc aggcccgtcg ccgccccggg
      841 ggcggccagc acctcgacgc cgaacccgcg atgccccagc gcatgggcca gctccgccgc
      901 gctgaacgcg cggcctgcgg ggcggacgcc gatcgcctcg gcgtggatgc ggccgcgcgc
      961 gctcaccgcc aacaggcccc ccgggcgcag cacccgcgcg gcctgcgcga gcagcccctc
     1021 cgcgtgctcg ccggagtggc cgagctcgtc gtccaccagc accacgccgc cgagggcggc
     1081 cgacggctgg tcctccacgc gcccggccag cacccgccag ccgtcgtcga tcagcacgga
     1141 cgcgacggcg ggggagggca cacagacggg ctcttcgggg tcggcgtgct cgcgcagcac
     1201 atcgagcagc aggggacgca cgtggctagc cgcaggcccg cttcacatac gggtagggat
     1261 tcacgttgcc gccgccaccc ggcatcacct cgaagtgcag gtgcggggtg ccctgggcgt
     1321 tgccggtgtc gccgttgaag gcgatgtgct cgccggcgtt caccgcctgc ccggcgctca
     1381 gcccgctgac gtagccctgc aggtgggtgt agtagtacgt gttcccgctc gcgccgcgca
     1441 tgtagatgga gatgccgccg acgcgcgagt tggtgagccg ggtgatgctc ccgctctcgt
     1501 aggcgtagat gccggtgccg cgcggggcca ggatgtcggt gcccttgtgc cggcggccgc
     1561 cggagcgcgg cgccccccac gtgtcgctgt agctgcgcgg gttgcccacc gggcaggcgg
     1621 cgccgttgac cacggcggcc gcaccttccg ccgcgcgggt gccggactgg cgcgtggtgc
     1681 gccgagccac ccgccgcttc gccgcctgct ccttggcgat gcggtcgcgc acgtggcgct
     1741 cgcgctgctg tgccttggcg agcaggccgg cgacctgggc gcgcaccgcg tcgagcctgg
     1801 cctggcggct gcgcaggtcc gcggcgacgc gctcgacgtc cagtgcggtc gcctgcgtgc
     1861 ggatccgggc cgccgtggcc gactcgaagt cggagcggct gcgcttggcc aggagcccca
     1921 gcagccgggc ctgggcggcc gcctgctgcg gggactcact ggtgaacagg gccagcatgg
     1981 ggtcgccggt gccgcgcttg taggactcgc gcacgcgcag ccgcagcgcc gcggacgcgc
     2041 cggctgcctg ctcctgcgcg cgtcgctcgg ccgctgccag ctccgcgagc cggttctcca
     2101 cctgcgcgac ctcggcttcc agcgtcgaca gccgcccgac cagcttgtcg agctcgcgct
     2161 gggcctgcac gcgctgctgc tgggccttct ccaggcgcgc atcggcggcc tgagcgggta
     2221 cgcccaggcc ggacagggcc agagatgcga cgagcacgcc gagaaggaaa cggggaagcg
     2281 gcgagcgtgg catgcgagac tgcgcgcgca ttgccttaga gggtagccgt cccgctgcgc
     2341 gaagaccaac accgcgacgg ccctcccggg gcgcacacct ccgccgccac gccggccggg
     2401 agcccccagg gggccgcgat gacggtgatg tccggcccgc ccggctcgtc ggaccagcgg
     2461 tccggcgact ccacctcgat ccgctccagt ggcggcggct cggcctcggg cagcgcgccg
     2521 tcctccgggc ggaccacctc agtgacgcgc catccggccg ccctgcgctc cagtcgcagc
     2581 gccagcacac ccgtgcgctg gggcccgtgg gccgtcaccg ccgcctcgaa ggcgtccggt
     2641 gccgccagag agccgcgggc cgcgccggga cgccgcatgg gcccgccccg cacccagccg
     2701 tcgcccaggc gcccggccag gcgcggcgtc atcagcggct cgaccgtcgc ccgcggccgc
     2761 cggccgcact ccacctccag gtacagctgg gcgaacgcca gcgccaagat cagcgggtcg
     2821 gcctcggact ccagcgtcat tgcgccacct ccacgcacgc gggctgcggc gccggaaccg
     2881 ccgccagttc ggcgggggcg gcgggagagg ggaccgccac aggttccggc gccggcggcg
     2941 cagcccggct cgtcagcagc agcgcgcaca acagcccggc gaccacccac ggggccgcgg
     3001 tgccccagga ccagcgcggc ctgccgtcgt cctgctcggg gggcgcaacc cacggggccg
     3061 cgggcgggta cggcggcggg ccggccggcc acgcggcggc gccgtcgcgc agccagtcgc
     3121 ccatcggccc cgcggcgccc tgcgg
//
LOCUS       DATMKC010000342         4032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_264395, whole genome shotgun sequence.
ACCESSION   DATMKC010000342 DATMKC010000000
VERSION     DATMKC010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264395"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VNU01_10055"
     CDS             complement(<1..423)
                     /locus_tag="VNU01_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33001.1"
                     /translation="MAAIPLFRTAGAAPGALRLTPAIAVLAMAASAAGVLLPGTYAEE
                     APAWAAQATGQDLVNLLLVYPFLLALARLARRGSLPAYLAWLGLLGYTAYSYLIYAGY
                     TRFSAMFPLYVAVLALSGWGLVAGLLPLRPDMVATAFRN"
     gene            650..1063
                     /locus_tag="VNU01_10060"
     CDS             650..1063
                     /locus_tag="VNU01_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWH33002.1"
                     /translation="MVARGDGGRRLERLSVAQCLALLGPSGIGRIAFAHGPTPVIMPV
                     NYAMDGGSLVLRTAEGTLLAAAPDGTGVALEVDRIDLDYHIGWSILLRGRMDGIRDAD
                     ELARCRDLPLGVYAPGLREAWRRITPATLTGRRIA"
     gene            1081..2214
                     /locus_tag="VNU01_10065"
     CDS             1081..2214
                     /locus_tag="VNU01_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWH33003.1"
                     /translation="MTIAEQRLRGLADAAAALAGELSLSVLLQTIIDTATEVTGARYG
                     ALGVLHEDDTISQFITTGADDETIRAIGHYPTGKGILGLLIRHPQVLRMENLASHPAS
                     HGFPANHPPMSTFLGAPVRSGGRVYGNLYLTEKDGGFDDIDEQIILVLAAQAGAAIEN
                     AQLSDRLQVLAVQDERARISRELHDGVIQSLFSIGLALESARPFLESDPERVRERLDM
                     AVESLDDVIRELRASIFRLVPHKAGELGLARGLTELAREFEVNALVRPDLHIPAGIDE
                     VLPAEWAPELLLVARELLSNCVKHASATAVAVTLAVREEHLVLEVRDNGVGFVPETPK
                     VGRGLDNVRQRALMLGAELDIRSAPGEGSTIQVRVPLHDLPSS"
     gene            complement(2225..3385)
                     /locus_tag="VNU01_10070"
     CDS             complement(2225..3385)
                     /locus_tag="VNU01_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWH33004.1"
                     /translation="MRRMLYESRMVPMPAAALEALLLERGPELLAQAAGSELYRDDTD
                     IDGSFGIDLPAQWAGMEFAKRVQVTIGVARRVAGRLMLPLSWEGEPGRHLFPVFEGTL
                     EVGPMFRDLCELTLAGSYRVPLGPVGAALDATVLHATARDTAARLVQALASVVTEDAP
                     PVESVPPSQGATPLRVRHVMTRDPLVVPAETGLRSATLALLSAGISAVPVVAADGGLL
                     GVLSEHDLLAKEARRRFGFGGGTRAEERRREALTAGEACTTPALVTTPETLLADVARQ
                     MLDRGINRLVVVEHGGIVGIVSRRDVLEALVRDTEDVVSAVRAALVAIGAEELDVRIG
                     DDGVVVLSGTVPLRSVAAQAGEAAAAVEGVMGVRADEVAYREDDVLPVMPMV"
     gene            complement(3402..>4032)
                     /locus_tag="VNU01_10075"
     CDS             complement(3402..>4032)
                     /locus_tag="VNU01_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWH33005.1"
                     /translation="RARVEPAGPEHAWLVVTGAYAPPRDAAGRPMDVTARRDGAREQA
                     RALARALAAELLAAAAPLHGVVAFPALEPAPPRVADVMDVDTPAVDEDLDLHAAASLL
                     EAAGVDGGPVVNASGRLVGVLTERDLLAGVVQPQDSHAPLAGALCRRPALVTVPQLSA
                     ARAAQQMLYHGVRRLAVVEDGRLVGMVTRTALFAALRAHAARTAAVTSV"
BASE COUNT          550 a         1493 c         1507 g          482 t
ORIGIN      
        1 gttccggaac gccgtcgcga ccatgtccgg ccgcagcggc agcagcccgg cgacgagccc
       61 ccatcccgac agggcgagca cggccacgta cagggggaac atggcgctga agcgcgtgta
      121 gcccgcgtag atcaggtagg agtaggcggt gtagcccaac aggcccagcc acgccaggta
      181 cgcgggcagc gagccccgcc gggccaggcg ggccagggcc agcaggaagg ggtagaccag
      241 caagaggttc accaggtcct ggcccgtggc ctgtgccgcc caagccggtg cctcctcggc
      301 gtaggtgcct ggcagcagca caccggcggc actggcggcc atggccagga cggcgatcgc
      361 cggggtcagg cgcagggcgc caggtgcggc gccggcggtc cggaacaggg ggatggcggc
      421 catgtgctgc ctccttcggg catgccctcg cggatcacgc ccaggctccg ctccccggac
      481 cgccccggcg acggctgaag gtcccgatcc gcggcgccgg acggcccgcg gcggcaccgg
      541 tcctggcgcc ggtgggactt tggtcccgct gcagcgggca cagcggcggt tgtgcgacgg
      601 cggtcccggc gccgagcatg ggcggatgag cgcacactgc agcgactcgg tggtggcccg
      661 cggcgacgga ggccggaggc tcgagcggct gtccgtggcg cagtgcctcg cgttgctcgg
      721 cccctccggc atcggcagga tcgcgttcgc gcacggcccc acgccggtga tcatgccggt
      781 caactatgcc atggacggcg gcagcctggt gctgcgcacc gccgagggca cgctgctcgc
      841 ggccgcgccc gacggcacgg gcgtcgcgct cgaggtcgat cggatcgatc tggactacca
      901 catcggctgg agcatcctgc tgcgggggcg gatggacggc atccgcgatg ccgacgaact
      961 ggcgcgctgc cgggacctgc cgctgggcgt ctacgctccc ggcttgcgcg aggcatggcg
     1021 ccgcatcacg ccggcaaccc tgaccggacg ccgcatcgcc tgatcgccta ccgtggaggg
     1081 atgaccatcg ccgagcagcg cctgcgcggc ctggcggacg ccgccgccgc cctggccggc
     1141 gagctcagcc tgtcggtgct gctgcagacc atcatcgaca cggccaccga ggtgaccggc
     1201 gcccgctacg gcgctctggg ggtgctgcac gaggacgaca cgatctcgca gttcatcacc
     1261 accggcgccg acgacgagac catccgggcg atcggccact accccaccgg caaggggatc
     1321 ctgggcctgc tgatccgcca cccgcaggtg ctgcggatgg agaacctggc cagtcatccg
     1381 gcctcccacg gcttcccggc caaccacccg ccgatgtcga cgttcctggg ggcgccggtg
     1441 cgctccggcg gccgcgtgta cggcaatctc tacctcacgg agaaagacgg cggcttcgac
     1501 gacatcgacg agcagatcat cctggtgctc gcggcccagg ccggcgcggc gatcgagaac
     1561 gcccagctgt ccgaccgcct ccaggtattg gccgtgcagg acgagcgggc gcggatctcc
     1621 cgcgagctgc acgacggcgt catccagtcg ctgttctcga tcggcctggc gctggagtcc
     1681 gcgcggccgt tcctggagtc ggatcccgag cgggtgcgcg agcgcctcga catggcggtg
     1741 gagtccctgg acgacgtcat ccgcgagctg cgcgcgtcga tcttccgcct ggtgccgcac
     1801 aaggccggcg agctgggact ggccaggggg ctgacggagc tggcgcgcga gttcgaggtg
     1861 aacgccctgg tgcgccccga cctgcacatc cccgcgggca tcgacgaggt gctgcccgcg
     1921 gagtgggcgc ccgaactgct gctggtggcc cgggagctgc tgtccaactg cgtcaagcac
     1981 gcctcggcga cggccgtggc ggtgacgctc gcggtgcgcg aggagcacct ggtcctggag
     2041 gttcgcgaca acggcgtggg cttcgtgccc gagacgccga aggtcggccg cggactggac
     2101 aacgtgcggc agcgcgccct gatgctgggc gccgagctcg acatccgctc ggcgcccggg
     2161 gagggcagca ccatccaggt gcgcgtgccg ctgcacgacc tgccgtcgtc ctgaccgccg
     2221 ccggtcacac catcggcatg acgggcagca cgtcgtcctc ccggtaggcc acctcgtcgg
     2281 cgcgcacgcc catgacgccc tccacggcgg cggccgcctc gcccgcctgg gcggccaccg
     2341 agcgcagcgg gaccgttccc gacagcacca cgacaccgtc gtcgccgatg cgcacgtcga
     2401 gttcctccgc cccgatggcg accagcgccg cacggacggc cgagacgacg tcctcggtgt
     2461 cgcgcacgag tgcctccagc acgtcccggc ggctcacgat gccgacgatg ccgccgtgct
     2521 cgaccaccac caggcggttg atgccgcggt ccagcatctg acgggccacg tcggccagca
     2581 gcgtctccgg ggtggtgacc agcgcggggg tggtgcacgc ctcgcccgcc gtgagcgcct
     2641 cgcgccggcg ctcctcagcg cgggtgccgc cgccgaatcc gaagcgccgg cgggcctcct
     2701 tggccaagag gtcgtgctcg ctcagcaccc cgagcaggcc gccgtcggcg gcgacgacgg
     2761 gcacggcgga gatgccggcg gacagcagtg ccagcgtggc gctgcgcagg cccgtctccg
     2821 cgggcacgac cagcgggtcg cgggtcatca cgtgacgcac ccgcaggggg gtcgccccct
     2881 gcgacggcgg gacggactcg accggtgggg catcctcggt gaccacggag gccagggcct
     2941 ggaccaggcg ggcggcggtg tcgcgcgcgg tggcgtgcag cacggtcgcg tccagggcgg
     3001 cgcccacggg gcccagcggc acgcggtagg agccggccag cgtgagctcg cacaggtcgc
     3061 gaaacatggg cccgacctcc agcgtgccct cgaacacggg gaacaggtgc cgtcccggct
     3121 caccctccca cgacagcggc agcatcaggc gcccggcgac gcggcgggcg acgccgatgg
     3181 tgacctggac gcgcttggcg aactccatcc cggcccactg tgcaggcagg tcgatgccga
     3241 aggagccgtc gatgtccgtg tcgtcgcggt acagctcgct gcccgccgcc tgggccagca
     3301 gttccggccc gcgctcgagc aggagtgcct ccagcgcggc ggcgggcatc ggcaccatgc
     3361 gggattcgta cagcatgcgg cgcatcggag gatcctttcg gctacacgga cgtgacggcg
     3421 gcggtgcggg ccgcatgggc gcgcagcgcg gcgaacaggg cggtgcgggt gaccatgccg
     3481 accaggcggc cgtcctccac gacggccagg cggcgcacgc cgtgatagag catctgctgg
     3541 gcggcacgcg cggcggacag ctgcggcacg gtgaccagcg ccgggcgcct gcacagcgcg
     3601 ccggccagcg gcgcatggct gtcctgcggc tggaccaccc cggcgagcag gtcgcgctcg
     3661 gtgagcacgc cgaccagccg gccggacgcg ttcaccacag ggccgccgtc cacgccggcg
     3721 gcttccagca gcgacgcggc agcgtgcagg tccaggtcct cgtccaccgc cggcgtgtcg
     3781 acgtccatga cgtcggcgac cctcggcggc gccggctcca gggcggggaa cgccacgacg
     3841 ccgtgcaggg gcgccgccgc cgcgagcagc tccgcggcca gggcgcgcgc cagggcgcgg
     3901 gcctgctcac gggcgccgtc ccggcgcgcc gtgacgtcca tcggccgccc cgccgcgtcg
     3961 cgcggcggcg cgtacgcgcc ggtcaccacg agccaggcgt gctccggccc cgcgggctcg
     4021 acgcgcgcgc gg
//
LOCUS       DATMKC010000343         6610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_102445, whole genome shotgun sequence.
ACCESSION   DATMKC010000343 DATMKC010000000
VERSION     DATMKC010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102445"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VNU01_10080"
     CDS             complement(<1..917)
                     /locus_tag="VNU01_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33006.1"
                     /translation="MGLRGRLALFFVAITVVPLAVAVFTLRLQIDRQLEQRAAAELSS
                     VREAAVSILAGQRARAGDLATDLVHRNVGPVLAAGDPAAAEAFLARAVPDPSPERADF
                     VVLLDAGGRMLAMRDSGEGVEAAQVAAAVVERRVPPGLLLEVREVRGAPGGQAEHLLG
                     WVVAGFHVDQDLLERLAVGEGLAIVGAGQVLATTAPGEAAAVVAQAPPDGQVRAARAG
                     GRDLVVTAASLSGDAASADAPRLVAWADPHGESPALGIALLVLLPSTVAAAVFGWLLA
                     SAIVGPIRRAADVARAVAGGDLSRSLEPSG"
     gene            931..1533
                     /locus_tag="VNU01_10085"
     CDS             931..1533
                     /locus_tag="VNU01_10085"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWH33007.1"
                     /translation="MTAETPGDREQDPETRKARLRAAMRTARVELGAAERAAASAEVC
                     GLLQGMDELQGDGPYLLYAAFGDELSVDALIDALLRRGARVCLPRVDGADLLPCLIGG
                     LGGLAGGWRGVREPAADAPEIPVESLRAVVVPGLAFDEAGNRLGYGGGHFDRLLARLP
                     GDVPVIGVAFDVQVLDGLPAQAHDRPVHAVVTESRIVRVR"
     gene            1624..1893
                     /locus_tag="VNU01_10090"
     CDS             1624..1893
                     /locus_tag="VNU01_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWH33008.1"
                     /translation="MPTYEYACQDCGEHLEVVQSFKEEPLSTCGACGGRLRKVFSAAG
                     IIFKGSGYYVTDSRKKSGGSDAGSGSGATPSTETKSDSAPKAESA"
     gene            1893..2705
                     /locus_tag="VNU01_10095"
     CDS             1893..2705
                     /locus_tag="VNU01_10095"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWH33009.1"
                     /translation="MTTPPQPSEQVIQVGVFGGSGFYDFLSDTEEIAVSTPYGEPSSP
                     VIVGEIGGVRVGFLPRHGADHALPPHKINYRANAWAMKSLGATDMILPCAAGSLQRDV
                     EPGHFVICDQVVDRTSGRVDTFYDGPETTHISFADPYDEEMRAVAIASAKSLGIPVHD
                     RGTIVVIQGPRFSTRAESKWFASMGWEVINMTQYPEVILVREMEMAALNISLITDYDV
                     GVEGGEAVSHHAVLETFKANNDKLRDLLFDLIPKLPRSADRPALHALEGARV"
     gene            2773..3444
                     /locus_tag="VNU01_10100"
     CDS             2773..3444
                     /locus_tag="VNU01_10100"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HWH33010.1"
                     /translation="MAGGGLARWRRALHGPSPVLPAWLDALSECWASLSARGRAVVAL
                     SGIVLFLSGCAWWAAGVAARWGGSPVPVLVAERQLAPGDAVTGLRLVELPPAAVPSAA
                     ATDVPEGARMAFALPAGSVLTQAHLDARGPGAALPEDQRAMPIPVEEGWRVTAGGWVD
                     VWVLGMEDRPSVLVAAACPVLAVAADDMGAGTALVALPRAAVGEVAQGLTAGRVLLAH
                     APPPG"
     gene            3495..4466
                     /locus_tag="VNU01_10105"
     CDS             3495..4466
                     /locus_tag="VNU01_10105"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWH33011.1"
                     /translation="MANWLQSLILGIVQGVTEFVPVSSSGHLVLLPYLAGWDTPGLAF
                     DVALHMGTALAVLVYFRRELWGMATAVVRRGDDPESALYRRLLVLLVVGSVPVALTGL
                     FLKPLFEDAFESPLLVSGFLVVTAVVLTAGERVRARRVGAVAPAPETGEHRARVWQGD
                     WHGSSEEVAVLEPAADVATGRDPADPHGVDLQRLGMRHALIVGAGQALALFPGLSRSG
                     TTITAGLFAGLTRPAATRFSFLLSLPALIGAGLVSLPDLGQEAARFSTGDILIGVGAA
                     FLAGYATVAWLVRLVARERLTVFVRYLLAAAVIGVFGYLMNGPLSSV"
     gene            complement(4463..5824)
                     /locus_tag="VNU01_10110"
     CDS             complement(4463..5824)
                     /locus_tag="VNU01_10110"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWH33012.1"
                     /translation="MSAEDREELLALLERVAGQATGEESIEAYGLDSTETTVRAFGGE
                     VESLSSARSRGVAIRVLRDARIGFAYTADLSEEGLRLALSDARENRAAASPDDANCLP
                     GAGDAGDLPELDAPGFAAVPPARKVELALALEAAARGAGGEVKGVDSAVYADAITTAA
                     IVSTSGIRAAYRRTDAYVFTEVLAERDGAATAAYGLDAARHPDGLDVEAAAAEGARRA
                     TRLLGARKPDSRKLPIVLDPFATASLLGVLAGALSAEAAQKGRSMFAGRVGERVGSER
                     VTLVDDGRLLDGPAAAPWDGEGVPTGRTPLIERGELRGFLHNTYTAAREGARSTGNAS
                     RSGFMSPPGLAPTNLFLEPGELDEAALLAEAGEAFYVQQIMGVHSGANAISGDISVGA
                     AGLMVRGGALAEPVREATIGGSIPALLEGLREVGSELRFLPFGGGMGGALLLVDGFTL
                     AGS"
     gene            complement(5821..>6610)
                     /locus_tag="VNU01_10115"
     CDS             complement(5821..>6610)
                     /locus_tag="VNU01_10115"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWH33013.1"
                     /translation="FDEHPPEEVGRLVARRAVTMLDARPAPSGEFTVVLAPGSGGVLF
                     HEACGHGMEADIVAKGASVYAGKRGQRLGTPILTGVDDATMPGHWGSFGFDDEGTPAQ
                     RTVLFADGVCTDYMSDRLRAAELGIPRSGNARRQSYAHLPIPRMTNSFILPGADDPAE
                     ILRGVKRGVWCEQLGGGQVDPASGDFVFGVAVGYWVENGEIAYPIRGANLVGDGPTVL
                     ARIDALGTDFDVRQGICGKDGQHVPAGLGNPTVRIDRITVGGTA"
BASE COUNT          779 a         2538 c         2442 g          851 t
ORIGIN      
        1 ccgctgggct ccagcgaccg gctgaggtcc ccgccggcca cggcgcgcgc cacgtcggcg
       61 gcgcggcgga tcgggccgac gatcgccgag gcgagcagcc agccgaacac cgccgccgcg
      121 accgtggacg gcagcaggac cagcagcgcg atgccaagcg ccggggactc gccgtgcggg
      181 tccgcccatg ccaccaggcg aggcgcgtca gccgaggcgg cgtccccgga cagcgacgcc
      241 gccgtcacca ccaggtcgcg gcccccggcc cgcgcggcac ggacctggcc gtccgggggc
      301 gcctgcgcca ccacggccgc cgcctcgccg ggcgccgtgg tggccagcac ctggccggcg
      361 cccacgatgg ccagcccctc gcccaccgcc agccgctcca gcaggtcctg gtccacgtgg
      421 aagccggcca cgacccagcc cagcagatgc tcggcctggc cgcccggcgc gccgcgcacc
      481 tcccggacct ccagcagcag ccccggcggc acgcgccgct ccaccacggc cgccgcgacc
      541 tgcgccgcct ccacgccctc tccggagtcg cgcatcgcca gcatccgccc gccggcgtcc
      601 agcagcacca cgaagtcggc gcgctcaggg gacgggtccg gcaccgcgcg cgccaggaac
      661 gcctccgccg cggccggatc gccggccgcg agcaccgggc cgacgttgcg gtgcaccagg
      721 tccgtcgcca ggtcgccggc gcgggcgcgc tggcccgcaa ggatgctcac cgccgcctcg
      781 cgcacgctgg acagctccgc cgcggcgcgc tgctcgagct gccggtcgat ctgcaggcgc
      841 agcgtgaaga cggccaccgc cagtggcacc accgtgatcg ccacgaagaa cagggcgaga
      901 cgcccgcgca gtcccatact gtgacgcatc atgacagccg agacgcctgg cgacagggag
      961 caggaccccg agacgcgcaa ggcgcggctg cgcgccgcca tgcgcaccgc gcgcgtggaa
     1021 ctgggcgcgg cggagcgggc ggcggcgtcc gcggaggtct gcggcctgct gcaggggatg
     1081 gacgagctcc agggggacgg cccctacctg ctgtacgcgg cgttcggcga cgagctgtcc
     1141 gtggacgcgc tgatcgacgc cctgctgcgc cgcggcgcgc gcgtctgcct gccccgggtg
     1201 gacggcgcgg acctgctgcc gtgcctgatc ggcggactcg gcgggctggc cggggggtgg
     1261 cgcggcgtgc gcgagccggc cgccgacgcg ccggagatcc cggtggagtc gctgcgcgcc
     1321 gtggtggtgc cgggcctcgc gttcgacgag gccggcaacc gcctgggcta cggcggcggg
     1381 cacttcgacc gcctgctggc gcgcctgccc ggcgacgtgc cggtgatcgg ggtggcgttc
     1441 gacgtccagg tgctcgacgg cctgcccgcg caggcccacg accggccggt gcacgcggtg
     1501 gtcacggaat cgcgcatcgt gcgggtgcgt tgacgggttt gccggagcgc ccggctgccg
     1561 acacaatttc acgagcgcgc gaacgcgcgc gggccttgag acacggtgct tttcggagga
     1621 acgatgccga cgtacgaata cgcctgccag gactgcggcg agcacctgga ggtggtgcag
     1681 tccttcaagg aggagccgct cagcacgtgc ggcgcctgtg gggggcggct tcgcaaggtg
     1741 ttcagcgccg ccgggatcat cttcaagggc tccggctact acgtgacgga cagccgcaag
     1801 aagtccggcg ggtccgacgc gggctccggc tcgggtgcca cgccgtccac cgagaccaag
     1861 agcgactccg cgcccaaggc ggagtccgcc tgatgaccac acccccgcag cccagcgagc
     1921 aggtcatcca ggtcggcgtc ttcggcgggt cgggcttcta cgacttcctc agcgacacgg
     1981 aggagatcgc ggtctccacg ccctacggcg agccgtcgtc gccggtgatc gtgggtgaga
     2041 tcggcggcgt gcgcgtgggt ttcctgccgc ggcacggcgc cgaccacgcg ctgccgccgc
     2101 acaagatcaa ctaccgggcc aacgcctggg cgatgaagtc cctgggcgcc acggacatga
     2161 tcctgccgtg cgccgccggg tcgctgcagc gcgacgtcga gcccggccac ttcgtgatct
     2221 gcgaccaggt ggtggaccgc acgtccgggc gcgtcgacac gttctacgac ggccccgaga
     2281 ccacgcacat ctccttcgcc gacccctacg acgaggagat gcgcgccgtc gcgatcgcct
     2341 cggcgaagtc cctcggcatc ccggtgcacg accgcggcac catcgtggtg atccaggggc
     2401 cgcgcttctc cacgcgcgcc gagtccaaat ggttcgcctc catgggctgg gaggtcatca
     2461 acatgaccca gtacccggag gtcatcctgg tgcgcgagat ggagatggcc gcgctgaaca
     2521 tctccctgat caccgactac gacgtcgggg tcgagggcgg cgaggcggtc agccatcacg
     2581 ccgtgctgga gacgttcaag gcgaacaacg acaagctgcg cgacctgctg ttcgacctca
     2641 tccccaagct gccgcggtcg gcggaccgcc cggcgctgca tgccctggag ggcgcgcgcg
     2701 tctgacgttt ttccacagga acgcgcgttg gcgaggtgac gcgagccgcc gcatgccgca
     2761 gggtgtggcc tcatggcagg cggcggattg gcgcgctggc ggcgggcgtt gcacggcccg
     2821 tcccccgtgc tgcccgcgtg gctggacgcg ctgtcggagt gctgggcgtc gctgagcgcg
     2881 cgcggccggg cggtcgtcgc gctgtcgggc atcgtgctgt tcctgtccgg ctgcgcctgg
     2941 tgggccgccg gggtggccgc acgctggggc ggctcccccg tgccggtgct cgtcgccgag
     3001 cgccagctcg cgccgggcga cgccgtgacc ggcctgcgcc tggtcgagtt gccgcccgcc
     3061 gcggtgccgt ccgcggcggc cacggacgtg cccgagggcg cgcgcatggc gttcgccctg
     3121 ccggcgggct cggtgctcac gcaggcccac ctggacgcgc gcgggccggg cgcggcgctg
     3181 ccggaggacc agcgcgccat gccgatcccc gtggaggagg gctggcgcgt gacggcgggc
     3241 ggctgggtgg acgtctgggt actcggcatg gaggaccgcc cgtcggtgct ggtggcggcg
     3301 gcgtgcccgg tgctggcggt ggccgccgac gacatggggg ccggcacggc gctcgtcgcg
     3361 ctgccgcgcg ccgccgtcgg cgaggtggcc caggggctca ccgccggccg cgtgctgctc
     3421 gcgcacgcgc cgccgcccgg ctgagcggcc gctgctagca tcgcccggtc ccagccccca
     3481 ccaccggagc accgatggcg aactggctgc agtccctcat tctcggcatc gtgcagggcg
     3541 tcacggagtt cgtgcccgtg tcgtcctccg gccacctggt gctgctgccc tacctggcgg
     3601 gctgggacac gccggggctg gcgttcgacg tcgcgctgca catgggcacc gccctggcgg
     3661 tgctcgtcta cttccggcgc gagctgtggg gcatggccac cgcggtggtg cgccgcggcg
     3721 acgacccgga gagcgcgctg taccggcggc tgctcgtgct gctggtcgtg ggcagcgtgc
     3781 cggtggcgct cacggggttg ttcctcaagc cgctgttcga ggacgcgttc gagtccccgc
     3841 tgctggtgtc cggcttcctg gtggtcacgg cggtggtgct gaccgcgggc gagcgggtgc
     3901 gcgcgcgccg cgtgggcgcc gtcgccccgg cgccggagac cggcgagcac cgggcgcggg
     3961 tgtggcaggg cgactggcac ggcagcagcg aggaggtcgc ggtcctggag ccggccgccg
     4021 acgtggccac cggccgcgat ccggccgacc cgcacggcgt ggacctgcag cggctgggca
     4081 tgcgccacgc gctgatcgtg ggcgccgggc aggcgctggc gctgttcccg ggcctgtcgc
     4141 gctccggcac caccatcacg gccgggctct tcgccgggct cacacgcccc gccgccacgc
     4201 gcttctcgtt cctgctttcg ctgccggcgc tgatcggcgc cgggctggtc agcctgccgg
     4261 acctgggcca ggaggcggcc cgcttcagca ccggcgacat cctcatcggc gtgggcgccg
     4321 cgttcctggc cggctacgcg acggtggcgt ggctggtgcg cctggtcgcc cgcgagcgcc
     4381 tgaccgtgtt cgtgcgctac ctgctggccg ccgccgtcat cggcgtgttc ggctacctga
     4441 tgaacggccc cctcagctcg gtctagctgc cggcgagggt gaagccgtcc acgagcagca
     4501 gcgcgccgcc catgccgccg ccgaacggca ggaagcgcag ctcgctgccc acctcgcgca
     4561 ggccctccag cagcgccggg atggagcccc cgatcgtcgc ctcgcgcacc ggctccgcca
     4621 gcgcgccgcc gcggaccatc agccccgccg cgcccacgct gatgtcgccc gagatcgcgt
     4681 tcgcccccga gtgcacgccc atgatctgct gcacgtagaa cgcctcgccc gcctccgcca
     4741 gcagcgccgc ctcgtccagc tcgcccggct ccaggaacag gttcgtgggc gccaggccgg
     4801 gcggggacat gaagccggag cggctcgcgt tgccggtgct gcgcgcgccc tcgcgcgccg
     4861 ccgtgtaggt gttgtgcagg aagccgcgca gctcgccccg ctcgatcagc ggggtgcgcc
     4921 ccgtgggcac gccctcgccg tcccacggcg ccgcggccgg gccgtcgagc agccgcccgt
     4981 cgtccaccag cgtcacgcgc tcgctcccca cccgctcgcc gacgcgcccg gcgaacatgc
     5041 tgcgcccctt ctgcgccgcc tcggccgaca gcgcgcccgc cagcacgccc aaaagcgacg
     5101 cggtggcgaa cgggtccagc acgatcggca gcttgcggct gtccggcttg cgcgcgccga
     5161 gcaaccgggt cgcgcgccgc gcgccctccg cggccgccgc ctcgacgtcc agcccgtcgg
     5221 gatggcgcgc cgcgtccagg ccgtaggccg cggtggccgc gccgtcgcgc tcggccagca
     5281 cctccgtgaa cacgtaggcg tcggtgcgcc ggtacgcggc gcggatgccc gacgtcgaca
     5341 cgatcgccgc cgtggtgatc gcgtcggcgt acacggcgct gtccacgccc ttgacctcgc
     5401 ccccggcgcc gcgcgccgcc gcctccagcg ccagcgccag ctccaccttg cgggcgggcg
     5461 gcacggcggc gaagccgggc gcgtccagct ccggcaggtc gccggcgtcc ccggcgcccg
     5521 gcaggcagtt ggcgtcgtcc gggctcgcgg ccgcgcggtt ctcgcgcgcg tccgacagcg
     5581 ccaggcgcag gccctcctcg ctcaggtcgg ccgtgtaggc gaagccgatg cgcgcgtcgc
     5641 gcagcacgcg gatggcgacg ccgcgcgagc gcgcgctgga cagcgactcc acctcgccgc
     5701 cgaacgcgcg caccgtggtc tcggtggagt ccaggccgta cgcctcgatg gactcctcgc
     5761 cggtggcctg gcccgccacg cgctccagca gggccagcag ttcctcgcgg tcctcggcgc
     5821 tcatgcggtg cctccgacag tgatgcggtc gatgcgcaca gtggggttgc cgagccccgc
     5881 cgggacatgc tggccgtcct tgccgcagat gccctggcgc acgtcgaagt ccgtgcccag
     5941 cgcgtcgatg cgcgccagca ccgtggggcc gtcccccacc aggttcgcgc cgcggatcgg
     6001 gtaggcgatc tcgccgttct ccacccagta gcccacggcc acgccgaaca cgaagtcgcc
     6061 cgaggccggg tccacctgcc cgccgccgag ctgctcgcac cacacgccgc gcttcacacc
     6121 gcgcaggatc tcggcggggt cgtcggcgcc cggcaggatg aaggagttgg tcatgcgcgg
     6181 gatcggcagg tgcgcgtagc tctggcgccg ggcgttgccc gagcgcggga tgccgagctc
     6241 cgccgcgcgc agccggtcgc tcatgtagtc ggtgcagacg ccgtcggcga acagcacggt
     6301 gcgctgcgcc ggcgtgccct cgtcgtcgaa gccgaagctg ccccagtggc ccggcatcgt
     6361 ggcgtcgtcc acgcccgtga ggatcggcgt gccgaggcgc tgcccgcgct tgccggcgta
     6421 gacgctggcg cccttggcga cgatgtcggc ctccatgccg tggccgcacg cctcgtggaa
     6481 cagcaccccg ccgctgccgg gggccagcac cacggtgaac tcaccgctcg gcgcgggccg
     6541 cgcgtccagc atcgtcaccg cccggcgggc gaccagccgg ccgacctcct ccggcggatg
     6601 ctcgtcgaac
//
LOCUS       DATMKC010000344         9333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_157997, whole genome shotgun sequence.
ACCESSION   DATMKC010000344 DATMKC010000000
VERSION     DATMKC010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157997"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /gene="dtd"
                     /locus_tag="VNU01_10120"
     CDS             <1..343
                     /gene="dtd"
                     /locus_tag="VNU01_10120"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWH33014.1"
                     /translation="RSTEADADWLASKTADLRVFADADGRMDRSVRDIGGGVLAVSQF
                     TLYGEVAKGRRPSFVAAAEPERARALYERYCEALRARGVGTAQGVFGAHMVIELVADG
                     PVTLRMGRETG"
     gene            519..1598
                     /locus_tag="VNU01_10125"
     CDS             519..1598
                     /locus_tag="VNU01_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWH33015.1"
                     /translation="MTRPRYSKAAPRRHDAGAQGGAVRDAVVERLLTLAREELGADIA
                     YLEPLHPAALEPPPSGAVDVPLPLPEGKSGGMLRFTPSADAAEAERDVQAIRALAAML
                     GSQLEAPALDRESWQLRIMRIRDLLDHGDLPVVFQPIANLQTGEIVGVEALSRFPGSS
                     DHAPDKWFNEAASVGLGVELEVLAVRRALNALDQLPESAYLSVNVSPAVAMSAGLAET
                     LDEVPLHRVVLEITEHSQVLDYDRLNLALVALRARGVRLAIDDAGSGFSSLRHILRLS
                     PDIIKLDMSLTQGIDNDSVLRALGYSLASFASAIDAAVIAEGVETERELHALRFLQVS
                     YGQGFFLRRPGPMPMPDRLDGEVFG"
     gene            complement(1633..3321)
                     /locus_tag="VNU01_10130"
     CDS             complement(1633..3321)
                     /locus_tag="VNU01_10130"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWH33016.1"
                     /translation="MRLALIQSNPVVGDIGGNAAALLDAYWRACERGADLVVSPELAL
                     TGYPPEDLVLKSAFVDANVDSLRRLAGAVGGVPFVVGFVERLPGREATDLLGEYDHLA
                     NAAAVLRDGAVEAVYRKQRLPNYGVFDEKRYFHAGDEAVVFDVGGVPVGVTVCEDQWA
                     ADGPVAQAAAAGARVVVNVNASPFVIGKRAARDRWALRHTTTHGVWFAYLNMVGGQDD
                     VVFDGDSFLMAPSGRVVARAAQFSEDLLLADIPASLAEGDGDWPEPAQRLDPDGEAYT
                     ALVLATRDYARKNGFSRALLGLSGGVDSALVAAIAADALGADRVTTVAMPSPYSSQGS
                     LTDAEALAANLGVRHLVIPIDAPMKAFDEALGELFAGTASGLAEENVQSRIRGSLLMA
                     ISNKFGDLLLSTGNKSEYAVGYATLYGDMAGGFAPIKDVSKTRVYRLCRWRNAVEGRD
                     LIPRAILDKEPSAELRPDQRDSDSLPPYQEVLDPILECYVERDRSVAEIVAETGLDAG
                     VVAAVAALVDRAEYKRRQSAPGPKITPKAFGKDRRVPITQAWTLAPGATGDAET"
     gene            3466..4788
                     /locus_tag="VNU01_10135"
     CDS             3466..4788
                     /locus_tag="VNU01_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWH33017.1"
                     /translation="MELTLEGLENNVLILFIMLASVLVFIMHVGFTMLEAGFTRAKNS
                     ANIIAKNLMTLCLGFLVYWAVGFAIMYGASASGLFGTDGFFLLNFSEYEPLAGSIAID
                     FLFQGMFAATAATIVSGAVAERMKFGVYGFVAVAMTALIYPVIGHWKWGGGWLAELGF
                     LDFAGSTVVHLTGGVAAFVGALILGPRIGKFAKDGTARAIPGHSMPLGALGVLILFFG
                     WFGFNGGSVLALDGPVIASVLLTTAIAGAAGGVGAGYWTRLRYGKFDVGMLGNGILAG
                     LVGITAGADVVNNLGAVLVGLLAGVVVALAVPLVDKLKVDDPVGAVSVHGVCGALGTL
                     WVGLMHTEEGLFYGGGATLLGVQAVGVLATIAYVGVVTAVLFSVLKATVGIRVSEQEE
                     IEGLDIHEHGMYGYPELALGPAAYPGGPATETSGPAMAPVPKTAFQTT"
     gene            4915..6240
                     /locus_tag="VNU01_10140"
     CDS             4915..6240
                     /locus_tag="VNU01_10140"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWH33018.1"
                     /translation="MDKQQEYVLRTVEERDIRFVRLWFTDVLGMLKSVAVTSSELEGA
                     FAEGIGFDGSAVDGFARIQESDMLAVPDASTFQVLPWREDAGGTARMFCDIRTPQGEP
                     FAADPRQVLRRNLAKAADMGFSFYVHPEMEFFLFAGPNDPTPLDNGGYFDLTPLDVQS
                     DFRRQAIQTLEGMGISVEYSHHEVAPSQHEIDLRYADALTMADNIMTYRLVVKEVAMQ
                     RGIYATFMPKPLAEHWGSAMHTHVSLFDGDANAFHDNDDPYHLSDTGKQFIAGLLTHS
                     REITAICCQWVNSYKRLVPGFEAPVYVSWGRHNRSSLIRVPMYKPNKPASARVEFRAP
                     DPACNPYLAFAVILAAGLRGIEKGYELPAETEDNIYQMTEQERRAAGIGSLPRNLDEA
                     LRVMEDSELVAEALGEHVFEYFLRNKRAEWDEYRAQVTPFELAKYLPTL"
     gene            6249..9098
                     /locus_tag="VNU01_10145"
     CDS             6249..9098
                     /locus_tag="VNU01_10145"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HWH33019.1"
                     /translation="MGADASRASLAAAGLDADAIAQAELAAGDPEAARRALAAVAGRD
                     PLVLRAGLGHELVRRIGAVAGASDALGALLGQSAAAVAVLAGDLAPQDAATVRGACDD
                     ALDGSADPARDLARVQRLGLLRIAARDLLGMADTPTVAAELADLAQGVLASALDQVVR
                     EVGGPGRLAVIAMGKLGGRELNYVSDVDVMFVHDGDFAAAAKLCERYLRLLSTVSPEG
                     RAYEVDANLRPEGKDGPLSRSISGFRTYYERWARNWEFQALLKARPIAGDPELGQEFL
                     AAIEPFVWPDRRPPEAIAEIQAMKTVVEESAPVRKAGDRQVKLAPGGLRDIEFAVQLL
                     QLVHGRHDPALRSANTLESLAALAAGGYVDEEDAEVFDEAYRFLRTVEHRLQLVRLRR
                     THTIPEDAGERRRLARSMGFRDSKDDGPLERFDRELARVRGVVRQLHEKLFYRPLLDR
                     FAQVGADDALPVSEGGLAEQAARERLEALGFANATAALAHLDALAGGVTRRARLLRTL
                     LPAILPTLADQPDPDGGLAAFRSLADRLQESPTFLRTLRDNPPVGEILAQSLGRSRLV
                     GQWLERQPEVFGMLGDPGGLAQALEPADYRRLADGLLRRGQDPAAGADALRRMRRREA
                     VRIAVRDLMGLADSVEVVKELSGLAQACLEAGLCLALDGHDEVRMAVIGMGKLGGAEL
                     GYASDLDVLLVFDGPRGPALAAASRLLKLLGDITPEGKAFVVDPNLRPEGKDGPLART
                     LESYAAYYERWAEPWELQALTQARPVAGDPQLGHAFVEMVAPHVYPSPVPAARLQAIR
                     LMKARVERERAGGGRGGARMGERADVKLGPGGLADIEWTVQLLQIKHGAGHASLRVPG
                     ALSGVEALAALGLLGEDDADWLRDGLRLLWALRNALYLAGFRDTQTLPANPEDQRRVA
                     RMLGWDPLATQAFLEELSRTMRRVRRVHERAFYDA"
     gene            9127..>9333
                     /locus_tag="VNU01_10150"
     CDS             9127..>9333
                     /locus_tag="VNU01_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWH33020.1"
                     /translation="MGIHEVIIEVGDVTTAVEFYTKVVGFKLVRVVEHGGRKVAELDA
                     DGQRVSLVGTGGPSVRFALRTADVR"
BASE COUNT         1226 a         3290 c         3494 g         1323 t
ORIGIN      
        1 gcgctccacg gaggccgacg ccgactggct ggcgtccaag acggcggacc tgcgcgtgtt
       61 cgcggacgcc gacggccgca tggaccgctc cgtgcgcgac atcggcggcg gcgtcctggc
      121 ggtcagccag ttcacgctct acggcgaggt cgccaagggg cggcggccgt cgttcgtggc
      181 ggccgcggag ccggagcggg cgcgcgcgct gtacgagcgg tactgcgagg cgctgcgggc
      241 gcggggcgtc ggcaccgccc agggcgtctt cggcgcgcac atggtcatcg agctcgtcgc
      301 ggacggtccc gtgacgctcc ggatggggcg cgaaacggga tgaatcgctg tgactcccga
      361 aaactacttg acggatgcgt tgctcatggg gtgggatgtg cgcttgttac gtatgttgct
      421 cctgtgacga ccgggacgca gcctggccgc acaccgcgtg cggacatcac acctcaggga
      481 cgagtgggat gcgaggcggg caaggacgca cgtcgatcat gacgaggccg agatacagca
      541 aggcggcccc gcgccgccat gacgcgggcg cgcagggcgg cgcggtgcgt gacgccgtgg
      601 tggagcgatt gctcacgttg gcgcgcgagg agcttggcgc cgacatcgcc tacctggagc
      661 cgctgcatcc cgccgcgctc gaaccgccgc catcgggtgc ggtggacgtg ccgctgccgc
      721 tgccggaggg caagtccggc ggcatgctgc gcttcacgcc ctcggccgac gccgccgagg
      781 ccgagcgcga cgtccaggcc atacgcgccc tggccgccat gctcggctcg cagctggagg
      841 cgccggcgct ggaccgcgag tcctggcagc tgcgcatcat gcgtatccgc gacctgctgg
      901 accacggcga cctgccggtg gtgttccagc ccatcgcgaa cctgcagacc ggggagatcg
      961 tgggcgtgga ggcgctgtcg cgcttcccgg gcagcagcga ccacgcgccc gacaagtggt
     1021 tcaacgaggc cgcgtcggtg ggcctgggcg tcgagttgga ggtgctggcc gtccgccggg
     1081 cgctgaacgc gctggaccag ctgcccgagt cggcctacct gtcggtcaac gtctcgcccg
     1141 ccgtggcgat gtcggccggg ctggccgaga cgctggacga ggtgccgctg caccgggtgg
     1201 tgctggagat caccgagcac tcgcaggtgc tggactacga ccggttgaac ctggcgctcg
     1261 tggcgctgcg cgcgcggggg gtgcgcctgg ccatcgacga cgcgggctcg ggcttctcca
     1321 gcctgcgcca catcctccgg ctgtccccgg acatcatcaa gctggacatg tcgctgacgc
     1381 agggcatcga caacgactcg gtgctgcgcg cgctgggcta ctcgctggcc tcgttcgcgt
     1441 ccgcgatcga cgcggccgtc atcgccgagg gcgtggagac cgagcgcgag ctgcacgccc
     1501 tgcgcttcct ccaggtcagc tacggccagg gcttcttcct gcgccggccc gggccgatgc
     1561 cgatgcccga ccgcctggat ggcgaggtgt tcgggtgagc ctgtcggcgt gggacgcgcc
     1621 cgcgtccgcg cgttacgtct cggcgtcgcc ggtggctccc ggcgccagcg tccacgcctg
     1681 ggtgatcggc acgcgccggt ccttgccgaa ggccttgggc gtgatcttgg ggcccggcgc
     1741 ggactggcgc cgcttgtact cggcgcggtc caccagtgcg gcgaccgccg cgaccacgcc
     1801 ggcgtccagc cccgtctccg ccacgatctc cgccacggag cggtcccgct ccacgtagca
     1861 ctccaggatc gggtcgagca cctcctggta cggcgggagg ctgtcgctgt cgcgctggtc
     1921 cggccgcagc tccgcggacg gctccttgtc caggatcgcc cgcgggatca ggtcgcggcc
     1981 ctccacggcg ttgcgccagc ggcacagccg gtacacgcgt gtcttggaca cgtccttgat
     2041 cggcgcgaag ccgccggcca tgtcgccgta cagcgtggcg tagccgaccg cgtactcgct
     2101 cttgttgccc gtgctgagca gcaggtcgcc gaacttgttg gagatcgcca tgagcaggct
     2161 gccgcggatg cgcgactgca cgttctcctc ggccaggccg gacgccgtgc cggcgaacag
     2221 ctcaccgagc gcctcgtcga aggccttcat gggcgcgtcg atggggatga cgaggtggcg
     2281 gacccccagg ttggcggcca gcgcctccgc gtcggtcagc gagccttgcg aggagtacgg
     2341 cgacggcatg gccacggtgg tcacgcggtc cgcgcccagc gcgtcggcgg cgatggcggc
     2401 caccagcgcg gagtcgacgc ctccggacag gccgagcagc gcgcgggaga agccgttctt
     2461 gcgcgcgtag tcgcgcgtgg ccagcaccag ggccgtgtag gcctcgccgt cggggtcgag
     2521 ccgctgcgcc ggctccggcc aatcgccgtc gccctccgcc agtgacgcgg ggatgtcggc
     2581 caacagcagg tcctcgctga actgtgccgc gcgcgcgacc acccgtccgc tgggggccat
     2641 gaggaacgag tcgccgtcga agaccacgtc gtcctgtccg cccaccatgt tgaggtaggc
     2701 gaaccacacg ccgtgcgtgg tcgtgtggcg cagtgcccag cggtcgcggg cggcgcgctt
     2761 gccgatcacg aaggggctcg cgttcacgtt gaccacgacg cgcgcgcccg cggcggcggc
     2821 ctgcgcgacc gggccgtcgg ccgcccactg gtcctcgcag acggtcacgc cgaccggcac
     2881 cccgcccacg tcgaagacca ccgcctcgtc gccggcgtgg aagtagcgct tctcgtcgaa
     2941 cacgccgtag ttgggcaggc gctgcttgcg gtagaccgcc tccaccgcgc cgtcgcgcag
     3001 caccgccgcg gcgttggcga ggtggtcgta ctcgcccagc aggtcggtcg cctcccggcc
     3061 gggcaggcgc tccacgaagc ccaccacgaa cggcacgccg ccgaccgccc cggccaggcg
     3121 ccgcagggaa tccacgttgg cgtccacgaa cgcgctcttg aggaccaggt cctccggcgg
     3181 gtagccggtg agcgcgagct ccggcgacac gaccaggtcc gcgccgcgct cgcacgcgcg
     3241 ccagtacgcg tcgagcagcg cggcggcgtt gccgccgatg tcgccgacga cggggttgga
     3301 ctggatcagg gccaggcgca tcagtcggac ctcgggaaac ctcggattta cacgggagaa
     3361 acggacgggc aactgccgcg actcgggagc ttcctacctt ctgcatccgt agcgagtgga
     3421 tgggttgggg cctgccgctc tcggtgcgcg aggaaaggtg cggccatgga actcactctc
     3481 gaaggactcg agaacaacgt cttgatcctg ttcatcatgc tcgcctccgt gctggtgttc
     3541 atcatgcacg tgggcttcac catgctggag gcgggcttca cgcgggcgaa gaactccgcg
     3601 aacatcatcg ccaagaatct catgaccctg tgcctgggct tcctggtcta ctgggcggtc
     3661 ggcttcgcga tcatgtacgg ggcgtccgcc tccgggctgt tcggcacgga cgggttcttc
     3721 ctgctcaact tcagcgagta cgaaccgctc gccgggtcga tcgcgatcga cttcctgttc
     3781 cagggcatgt tcgcggccac ggcggccacg atcgtgtccg gggcggtggc cgagcgcatg
     3841 aagttcggcg tgtacggctt cgtggccgtc gcgatgaccg cgctgatcta cccggtcatc
     3901 ggccactgga agtggggcgg cggctggctc gccgagctgg gcttcctgga cttcgccggc
     3961 tccacggtcg tgcacctcac cggcggcgtc gccgcgttcg tgggcgcgct catcctgggg
     4021 ccgcgcatcg gcaagttcgc caaggacggg accgcccgcg cgatccccgg ccactccatg
     4081 ccgctgggcg cgctgggcgt gctgatcctg ttcttcggct ggttcggctt caacggcggc
     4141 tccgtgctcg cgctggacgg cccggtgatc gcctcggtcc tgctcaccac cgccatcgcc
     4201 ggcgccgccg gcggcgtggg cgcgggctac tggacgcggc tgcgctacgg caagttcgac
     4261 gtgggcatgc tcggcaacgg catcctggcc ggtctggtgg gcatcaccgc cggcgccgac
     4321 gtggtcaaca acctgggcgc cgtgctggtc ggcctgctgg ccggtgtcgt ggtcgcgctg
     4381 gccgtgcccc tggtggacaa gctcaaggtc gacgacccgg tcggcgccgt gagcgtgcac
     4441 ggcgtgtgcg gtgccctggg cacgctgtgg gtgggcctca tgcacaccga ggaaggcctg
     4501 ttctacggcg gcggcgcgac gctgctgggc gtccaggcgg tcggcgtgct cgcgaccatc
     4561 gcctacgtcg gggtcgtcac ggcggtcctg ttcagcgtgc tgaaggccac cgtgggcatc
     4621 cgggtctccg agcaggagga gatcgagggg ctcgacatcc acgagcacgg catgtacggc
     4681 taccccgagc tcgcgctcgg cccggcggcc taccccggtg ggcccgccac ggagacttcg
     4741 gggccggcca tggccccggt cccgaagacc gcgttccaga ccacctgacc tcagccccca
     4801 ccggcgggtc aggcagggta gagcgcaccg gcggagccgg ccgcgaacgc ggccggctcc
     4861 gctagcattc tcctcccacg cgacgcgtga cgcgtccgca ccacggaggc cgaagtggac
     4921 aagcagcagg agtacgtgct ccgcaccgtc gaagagcggg acatccgctt cgtgcgactg
     4981 tggttcaccg acgtgctcgg catgctcaag tcggtggccg tcacgtcctc cgagctggag
     5041 ggcgccttcg cggagggcat cggcttcgac ggctccgcgg tggacggctt cgcgcgcatc
     5101 caggagtccg acatgctcgc cgtgccggac gcctccacct tccaggtgct gccgtggcgg
     5161 gaggacgccg gcggcaccgc gcgcatgttc tgcgacatcc gcaccccgca gggcgagccg
     5221 ttcgcggcgg acccccgcca ggtgctgcgg cgcaacctgg ccaaggccgc ggacatgggc
     5281 ttttccttct acgtgcaccc ggagatggag ttcttcctgt tcgccgggcc caacgacccc
     5341 acgccgctgg acaacggcgg ctacttcgac ctgacgccgc tggacgtgca gagcgacttc
     5401 cgccgccagg ccatccagac gctggagggc atgggcatca gcgtggagta cagccatcac
     5461 gaggtcgcgc ccagccagca cgagatcgac ctgcgctacg ccgacgcgct caccatggcc
     5521 gacaacatca tgacctaccg cctggtggtg aaggaggtcg cgatgcagcg cggcatctac
     5581 gccaccttca tgcccaagcc gctggcggag cactggggct cggcgatgca cacgcacgtg
     5641 tcgctgttcg acggcgacgc caacgccttc cacgacaacg acgaccccta ccacctgtcc
     5701 gacaccggca agcagttcat cgccgggctg ctcacgcact cccgcgagat caccgcgatc
     5761 tgctgccagt gggtcaacag ctacaagcgc ctggtcccgg gcttcgaggc gcccgtctac
     5821 gtgagctggg gccggcacaa ccgcagctcg ctcatccgcg tgccgatgta caagcccaac
     5881 aagccggcgt cggcgcgtgt ggagttccgc gcgcccgacc cggcgtgcaa cccgtacctg
     5941 gcgttcgcgg tgatcctcgc cgccgggctg cgcggcatcg agaagggcta cgagctgccg
     6001 gccgagaccg aggacaacat ctaccagatg accgagcagg agcgccgtgc cgccggcatc
     6061 ggcagcctgc cgcgcaacct cgacgaggcc ctgcgggtca tggaggactc cgagctggtc
     6121 gccgaggcgc tgggcgagca cgtcttcgag tacttcctgc gcaacaagcg cgccgagtgg
     6181 gacgagtacc gggcgcaggt gactccgttc gagctcgcca agtacctgcc gaccctgtaa
     6241 agggcggcat gggggccgac gcttcgcgcg cctcgctcgc ggcggcgggc ctggacgccg
     6301 acgccatcgc ccaggccgag ctggccgcgg gcgatcccga ggcggcgcgc cgcgcgctgg
     6361 ccgccgtggc cgggcgcgac ccgctggtcc tgcgcgccgg actcggccac gagctggtgc
     6421 ggcgcatcgg cgcggtggcc ggcgccagcg acgcgctcgg cgcgctgctc ggccagtcgg
     6481 cggcggccgt cgcggtgctc gccggggacc tcgcgccgca ggacgccgcc acggtgcgcg
     6541 gcgcctgcga cgacgcgctg gatggctccg ccgatccggc gcgcgacctc gcgcgcgtgc
     6601 agcggctggg cctgctgcgc atcgccgcgc gcgacctgct ggggatggcc gacacgccga
     6661 ccgtcgccgc agagctcgcc gacctggcgc agggggtcct ggcctcggcc ctggaccagg
     6721 tggtgcgcga ggtgggcggg cccggccgcc tcgccgtcat cgccatgggc aagctgggcg
     6781 ggcgcgagct gaactacgtc agcgacgtgg acgtcatgtt cgtccacgac ggcgacttcg
     6841 cggccgcggc caagctgtgc gagcgctacc tgcggctgct gtccaccgtg tcgccggagg
     6901 gccgcgccta cgaggtggac gccaacctgc gcccggaggg caaggacggg ccgctgtcgc
     6961 gcagcatctc cggcttccgc acctactacg agcgctgggc gcgcaactgg gagttccagg
     7021 cgctgctcaa ggcgcggccc atcgccggcg acccggagct gggccaggag ttcctggcgg
     7081 ccatcgagcc gttcgtgtgg ccggaccggc gcccgccgga ggccatcgcg gagatccagg
     7141 cgatgaagac ggtggtggag gagtccgcgc cggtgcgcaa ggccggcgac cgccaggtga
     7201 agctggcgcc cggcggcctg cgcgacatcg agttcgcggt gcagttgctg caactggtgc
     7261 acgggcggca cgacccggcg ctgcgcagcg ccaacacgct ggagtccttg gcggcgctgg
     7321 ccgccggcgg ctacgtcgac gaggaggacg ccgaggtctt cgacgaggcg taccgcttcc
     7381 tgcgcaccgt ggagcaccgg ctgcagctgg tgcgcctgcg gcggacgcac acgatcccgg
     7441 aggatgcggg ggagcgccgc cgcctggcgc ggtcgatggg cttccgcgac agcaaggacg
     7501 acgggccgct ggagcgcttc gaccgcgagc tcgcgcgcgt gcgcggcgtg gtgcgccagc
     7561 tccacgagaa gctgttctac cggccgctgc tggaccgctt cgcgcaggtc ggcgcggacg
     7621 acgcgctgcc ggtgagcgag gggggcctgg ccgagcaggc ggcacgcgag cgcctggagg
     7681 cgctgggctt cgccaacgcc accgccgcgc tggcgcatct ggacgcgctg gccggcggcg
     7741 tgacgcggcg ggcgcggctg ctgcgcacgc tgctgcccgc gatcctgcca acgctggccg
     7801 accagccgga ccccgacggc ggcctggcgg cgttccgctc gctggcggac cggctgcagg
     7861 agtcgccgac gttcctgcgc acgctgcgcg acaacccgcc ggtgggcgag atcctggcgc
     7921 agtcgctggg ccgttcccgg ctggtggggc agtggctgga gcgccagccg gaggtgttcg
     7981 ggatgctggg cgatcccggc ggcctcgcgc aggcgctgga gcccgccgac taccggcgcc
     8041 tggccgacgg cctgctgcgc cgcggccagg acccggccgc gggcgcggac gcgctgcggc
     8101 gcatgcgccg ccgcgaggcc gtgcgcatcg ccgtgcgcga cctcatgggc ctggccgact
     8161 cggtggaggt ggtcaaggag ctgagcggcc tggcgcaggc gtgcctggag gcgggcctgt
     8221 gcctggcgct ggacgggcac gacgaggtgc gcatggccgt gatcggcatg ggcaagctgg
     8281 gcggcgccga gctcggctac gccagcgacc tggacgtgct gctggtgttc gacggcccgc
     8341 gcgggccggc gctggcggcc gcgtcgcggc tgctgaagct cctgggcgac atcacccctg
     8401 agggcaaggc gttcgtggtg gacccgaacc tgcggccgga gggcaaggac ggcccgctgg
     8461 cgcgcacgct ggagtcctac gccgcctact acgagcgctg ggccgagccg tgggagctgc
     8521 aggcgctgac gcaggcgcgg ccggtcgcgg gggacccgca gctggggcac gcgttcgtgg
     8581 agatggtcgc cccgcacgtc tacccgtcgc cggtgcccgc ggcgcggctg caggccatcc
     8641 ggctgatgaa ggcgcgcgtg gagcgcgagc gcgccggcgg cggccgcggc ggcgcgcgca
     8701 tgggggagcg ggccgacgtg aagctgggcc ccggcggact ggccgacatc gagtggacgg
     8761 tgcagctgct gcagatcaag cacggtgccg ggcacgcgtc cctgcgcgtg cccggcgcgc
     8821 tgtcgggcgt ggaggcgctg gcggcgctcg gcctgctggg cgaggacgac gccgactggc
     8881 tgcgcgacgg gctgcgcctg ctgtgggcgc tgcgcaacgc gctgtacctg gcgggcttcc
     8941 gggacaccca gacgctgccc gccaacccgg aggaccagcg ccgcgtggcc cgcatgctgg
     9001 gctgggaccc cctggcgacc caggcgttcc tggaggaact gtcgcgcacg atgcggcgcg
     9061 tccgccgcgt gcacgagcgc gccttctacg atgcctgagg cgcgacggtc gtgtgaagga
     9121 ggacgcgtgg gcatccatga ggtgatcatc gaggtcggcg acgtcaccac ggcggtcgag
     9181 ttctacacga aggtggtcgg cttcaagctg gtgcgcgtcg tggagcacgg cgggcgcaag
     9241 gtggccgagc tggacgcgga cgggcagcgc gtcagcctgg tgggcacggg cgggccgtcg
     9301 gtgcgcttcg ccctgcggac cgccgacgtg cgg
//
LOCUS       DATMKC010000345         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_350013, whole genome shotgun sequence.
ACCESSION   DATMKC010000345 DATMKC010000000
VERSION     DATMKC010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350013"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..652
                     /locus_tag="VNU01_10155"
     CDS             158..652
                     /locus_tag="VNU01_10155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWH33021.1"
                     /translation="MESDRTPGEHLGAAVDGPRAVDLRDYVVFDLDGVAGRRVLATDV
                     LAVDLLCLEPGQDVPPRTFTGTDVVYTVLGGVAWVVTDDAEVTLTPLQAVVIPAGVPH
                     AVRNDSPDPLILQALVSPPDEVAAPTGQPLRPAAAQPREVRGGRGGRAGGLRRLLGGR
                     GDGE"
     regulatory      664..775
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            769..>2104
                     /locus_tag="VNU01_10160"
     CDS             769..>2104
                     /locus_tag="VNU01_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33022.1"
                     /translation="MVDAVVAVPVLLAALALFAWLGVTARDSAADLDDYVAARGSQGA
                     VTVGLSFLASGAGAWILFTPAEIGAFVGMDGVAGYAVAAAAPFAAIALVGPRVRRLLP
                     RGRSLSDFAHERFGARFHLAVTAVSVLYMLTFLTAELTAVGALTERLTGLDGDVVLVL
                     VAAVTLAYTTVGGLRASLRTDTWQGWLVLALLAAGVLAAVGGLRDASAAWSGSGLLGV
                     DRVGIEVALTLLVAVTAANLFHQGYWQRVWAARDDAALRRGGALGAALTVPVLLVAGA
                     TGVLATGAGLDLGAPPAPFFALLTGLGPAVGVVVFVLGVALVASSVDTLQAGLSALTV
                     SELPGATLRRARVVTALAMVPVTAVALLGLSVLRIFLVADLLATAAVVPALIGLWSRA
                     TTAGAVAGLAAGLVAATIPEGLSGATFPSGVPTLAPFAWALAASAAVTVAASL"
BASE COUNT          195 a          779 c          813 g          317 t
ORIGIN      
        1 aatcgcggcg acacgccgct ggtagataac gtctgtagcg gtcacggtgc tctcctctga
       61 actgtggaag gtccagagag tcgagcaccg tgaccgcctc acacagcgga cccagtgtcg
      121 cccctacctg tgatccctac gtcctagact gcgtggcatg gaatcggacc gcacccccgg
      181 cgagcacctc ggggccgccg tcgacggccc ccgggccgtg gacctgcgcg actacgtggt
      241 gttcgacctg gacggggtcg cgggccggcg cgtcctggcc acggacgtcc tggccgtgga
      301 cctcctctgc ctggagcccg gtcaggacgt gccaccccgc accttcaccg gcacggacgt
      361 ggtctacacc gtcctcggcg gtgtcgcgtg ggtggtcacc gacgacgcgg aggtgacgct
      421 gacgcccctg caggccgtgg tcatcccggc gggcgtcccg cacgcggtgc gcaacgacag
      481 cccggacccg ctcatcctcc aggcgctggt gtcgccgccg gacgaggtcg cggcgcccac
      541 gggccagccg ctgcggcccg ccgccgcgca gccgcgggag gtgcgcggcg ggcgcggcgg
      601 gcgcgccgga ggcctcaggc ggctgctcgg cggccgcggc gacggagagt agactccgcg
      661 cgtcaatcac gccgggggag tccgcgcacg ggctgagagt gcggctgagg cggccgcaga
      721 ccccacgaac ctgacccggt tcacaccggc ggagggagtg cgtctgccat ggtcgatgcc
      781 gttgtcgccg tccctgtcct gctcgccgcg ctggcgctgt tcgcctggct cggtgtcacc
      841 gcacgcgact ccgctgcgga cctcgacgac tacgtcgccg cgcgaggctc gcagggcgcg
      901 gtgaccgtcg gcctgtcgtt cctcgcctcg ggcgcgggcg cctggatcct gttcaccccg
      961 gcggagatcg gggccttcgt cggcatggac ggcgtcgccg gctacgcggt ggcggccgcc
     1021 gcgccgttcg ccgcgatcgc cctggttggc ccgcgcgtgc gccggctgct gccgcggggc
     1081 cgcagcctgt cggacttcgc gcacgagcgc ttcggcgcgc gcttccacct cgccgtcacc
     1141 gccgtctcgg tcctgtacat gctgacgttc ctcacggcgg agctcacggc ggtcggcgcg
     1201 ctcacggagc ggctcaccgg cctggacggc gacgtggtgc tggtgctggt ggcggccgtg
     1261 acgctggcct acaccacggt gggcggcctg cgggcgtcgc tgcggacgga cacctggcag
     1321 ggctggctgg tacttgcgct gctcgccgcc ggggtcctgg ccgcggtcgg cggcctgcgc
     1381 gacgcctcgg cggcatggtc gggctccggg ctgctgggcg tggaccgtgt cggcatcgag
     1441 gtggcgctga cgctgctggt cgccgtcacg gcggcgaacc tattccacca gggctactgg
     1501 cagcgcgtgt gggccgcccg cgacgacgcg gcgctgcgcc gcggcggcgc gctgggcgcc
     1561 gcgctgaccg tgccggtgct gctcgtggcg ggcgcgaccg gcgtgctggc caccggggca
     1621 gggctggacc tgggcgcgcc gcccgcgccg ttcttcgcgc tgctgaccgg gctcggcccg
     1681 gcggtgggcg tggtggtgtt cgtgctcggc gtcgcgctgg tcgcctcgtc cgtggacacg
     1741 ctccaggccg gcctgtcggc gctgaccgtg agcgagctgc ccggcgcgac gctgcgccgc
     1801 gcgcgcgtgg tcacggcgct ggccatggtt cccgtgacgg cggtcgcgct gctcggcctg
     1861 tccgtcctgc gcatcttcct ggtggcggac ctgctggcca ccgcggcggt ggtgcccgcc
     1921 ctgatcgggt tgtggtcgcg cgcgaccacg gcgggcgcgg tggcgggcct ggccgccggc
     1981 ctggtcgcgg cgacgatccc cgaggggctg tcgggggcga cgttcccgag cggcgtgccg
     2041 acgctggcgc ccttcgcctg ggcgctggcc gcctccgccg ccgtcaccgt cgccgcctca
     2101 ctgg
//
LOCUS       DATMKC010000346         3821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_246826, whole genome shotgun sequence.
ACCESSION   DATMKC010000346 DATMKC010000000
VERSION     DATMKC010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246826"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..807
                     /locus_tag="VNU01_10165"
     CDS             229..807
                     /locus_tag="VNU01_10165"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HWH33023.1"
                     /translation="MRFSKEELQAQYGRTVPDLIGPDCRLLFVGINPGLWTAAVQTHF
                     ARPGNRFWPALHAAGITERQIDARTGFTSDDKDYLVRRGIGITNLVPRATARADELTH
                     AELVEGGDRLRRLVGVVKPKVVAVVGLTAYRVAFGIPKAEIGRQPESLEGAEVWAVPN
                     PSGLNAHYKPPELAEWYRRVAAAAGILRESTR"
     gene            complement(867..2012)
                     /locus_tag="VNU01_10170"
     CDS             complement(867..2012)
                     /locus_tag="VNU01_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWH33024.1"
                     /translation="MTTGPLRGVRIIEIAGIGPGPFAGMLLADMGADVIRVDRANPGG
                     LGAMFPPDKDVLIRGRRSIAVDMKSPEGIELVLSLAESADALFEGFRPGVMERMGLGP
                     DVCLARNPKLVYGRMTGWGQDGPWAQMAGHDLDYIALAGALHAIGAAGEAPPVPLNLI
                     GDFGGGGMFLAFGLVCGLLEASRSGQGQVIDAAMVDGTATLLAMFHGMLAMGMWRTER
                     GGNLLDGGAPFYRCYACADGGFVAVGALEPQFYAALLDGLGFAAEDWPQYDLARWGDL
                     REAFTKRFAERTRDEWAAVFAGTDACVAPVLSLTEAPEHEHLAARGVFVEHAGVRQPA
                     PAPRFSRTAPRLERPPVLPGQHTDEVLAELGLSPDRVAELRAAGTVK"
     gene            2228..2995
                     /locus_tag="VNU01_10175"
     CDS             2228..2995
                     /locus_tag="VNU01_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33025.1"
                     /translation="MGKLRTALTELGGITSIALDGVVAMRKRPFQGAEFLHQCWFLAK
                     VSAVPLILISIPFGMIIALHVGSFARQLGAEANVGSAMLLAVIREQAPVATALLIAGA
                     GGSAMCADLGSRRIRDEIDAMQVMGVDPMHRLVAPRLLAASVVAVFFVSIVAVAGILG
                     GWFFAVVVQKGTTGAYFASLNQLAQLPDYYMAVTKAVVFGFTAAAVACYKGYTVKGGP
                     KGVGDAVQASVVITFILLFFQNAVLTIMYLNLVPQKM"
     gene            3004..>3821
                     /locus_tag="VNU01_10180"
     CDS             3004..>3821
                     /locus_tag="VNU01_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33026.1"
                     /translation="MATAGPVIRIGRRIGGPFETVWDQFRFYGEVLRGLPTSFRYGSV
                     ILRLITDVTVGAGALILGGGMFFVIASLAFFTGAVVGFEGYVGLEQIGAQAYVGLVSS
                     WANTREITPLIAGVALAAQVGAGYTAELGAMRISEEIDALEVMSVPSIVYLVSTRVWA
                     ALIPIVPLYLMALFSSYYATRMTVTGYFGLSTGSYGQYFNLFLPATDVLYSVTKVIVF
                     AIVVVLIHCYYGYYASGGPAGVGVAVGRAIRTSIVAVVVLNLLLSILMWGGGDTV"
BASE COUNT          551 a         1399 c         1299 g          572 t
ORIGIN      
        1 ccgcgaccgc cgcgccaagg aacagaagac caacaaagac atcttgcgct gcctcaagcg
       61 ctacatcgcc cgagaagtcc accgcgccct gatggccgac ctcaccactc catcaccgac
      121 ttgacatcca taggagcatc ctccccggtt gggtgcactc cgggcgccat ctggcccaga
      181 ctgcacccgc acgaccggca aatctgtgga ctgggtagcc tcgcggccat gcgcttcagc
      241 aaggaggagc tccaggccca gtacgggcgc accgtgccgg acctgatcgg gcctgactgc
      301 cgcctgctgt tcgtcggcat caacccgggc ctctggaccg ccgccgtgca gacgcacttc
      361 gccaggccgg gcaaccgctt ctggccggcg ctgcacgccg cgggcatcac cgagcgccag
      421 atcgacgcgc gcaccggctt caccagcgac gacaaggact acctcgttcg ccggggcatc
      481 ggcatcacga acctggtgcc gcgcgccacc gcccgcgccg acgagctcac ccacgccgaa
      541 ctggtcgagg gcggggaccg gctgcgccgg ctcgtcggcg tcgtgaagcc gaaggtcgtc
      601 gcggtcgtgg gcctgacggc ctaccgcgtc gcgttcggga tccccaaggc ggagatcggc
      661 aggcagccgg agtccttgga gggcgcagaa gtgtgggcgg tgcccaaccc cagcggcctc
      721 aacgcccact acaagccgcc cgagctggcc gagtggtacc gccgcgtggc cgccgccgcc
      781 ggcatccttc gggaatccac acgctgacgc cctctatggg tgcggtttct gagcgatggc
      841 tctgagagtg cacccaacag gggggcctac ttgacggtgc cggcggcgcg cagctcggcg
      901 acgcggtcgg gggacaggcc cagttccgcc agcacctcgt cggtgtgctg ccccggcagc
      961 acggggggcc gctccaggcg cggcgccgtc cgcgagaagc gcggcgcggg cgccggctgg
     1021 cggacgccgg cgtgctccac gaacacgccc cgggccgcca ggtgctcgtg ctccggcgcc
     1081 tccgtcaggc tcagcaccgg cgccacgcac gcgtccgtcc cggcgaacac cgccgcccac
     1141 tcgtcgcgcg tgcgctcggc gaagcgcttc gtgaacgcct cgcgcaggtc gccccagcgc
     1201 gccaggtcgt actgcggcca gtcctccgcc gcgaatccca ggccgtccag cagcgccgcg
     1261 tagaactgcg gctccagcgc ccccacggcc acgaagccgc cgtcggcgca ggcgtagcag
     1321 cggtagaacg gcgcgccgcc gtcgagcagg ttgccgcccc gctcggtgcg ccacatgccc
     1381 atcgcgagca tgccgtggaa catcgccagc agcgtcgcgg tcccgtccac catcgccgcg
     1441 tcgattacct ggccctgccc ggagcgcgat gcctccagca gcccgcacac caggccgaac
     1501 gccaggaaca tgccgccgcc gccgaagtcg ccgatcaggt tcagcggcac cgggggcgcc
     1561 tcgccggccg ccccgatggc gtgcagcgcg ccggccaggg cgatgtagtc caggtcgtgg
     1621 ccggccatct gcgcccaggg gccgtcctgg ccccagccgg tcatgcgccc gtagaccagc
     1681 ttggggttgc gggccaggca cacgtccggc cccaggccca tgcgctccat gacgccgggg
     1741 cggaacccct cgaacagcgc gtcggcggac tccgccagcg acagcaccag ctcgatgccc
     1801 tcgggcgact tcatgtcgac ggcgatcgag cgccggccgc ggatcagcac gtccttgtcc
     1861 ggcgggaaca tggcccccag cccgcccggg ttggcgcggt ccacgcggat cacgtcggcg
     1921 cccatgtcgg ccagcagcat cccggcgaac gggccggggc cgatgcccgc gatctcgatg
     1981 atgcgcacac cgcgcagggg accggtggtc atggcgcgcc tttctccgtg ctgcccggga
     2041 gcgttgccgg tgatggaacg atttcagcct agtcgttcag ccgtcggcac aggaatcggc
     2101 gcaatctcga cacgacttgg tggcgaatcc gagcggaagg ggcgcgaaac cgctagaaca
     2161 gacacccgca ccgcgggagt gatcgtcaaa aggcgtccga gcaggggggc acccctaagg
     2221 gctgggcatg ggcaagctgc gcactgcgct gaccgaattg ggtgggatca cgtccatcgc
     2281 cctcgatggc gtcgtcgcca tgcgcaagcg cccctttcag ggcgccgagt tcctgcacca
     2341 gtgctggttc ctggccaagg tcagcgccgt cccgctgatc ctgatctcga tccccttcgg
     2401 catgatcatc gcgctgcacg tcggctcgtt cgcgcgccag ctcggcgccg aggccaacgt
     2461 cggcagcgcc atgctgctgg cggtcatccg cgagcaggct ccggtcgcca ccgcgctgct
     2521 gatcgccggc gccgggggca gcgcgatgtg cgccgacctg ggcagccggc gcatccgcga
     2581 cgagatcgac gccatgcagg tcatgggcgt ggacccgatg caccgcctgg tggcgccgcg
     2641 cctgctggcc gccagcgtcg tggcggtgtt cttcgtcagc atcgtggcgg tggccggcat
     2701 cctgggcggc tggttcttcg cggtggtggt gcagaagggc accacgggcg cgtacttcgc
     2761 cagcctcaac cagctcgcgc agctgccgga ctactacatg gccgtcacca aggcggtggt
     2821 gttcggcttc accgccgcgg cggtcgcctg ctacaagggc tacaccgtca agggcgggcc
     2881 caagggcgtc ggcgacgccg tccaggccag cgtggtgatc acgttcatcc tgctgttctt
     2941 ccagaacgcc gtcctgacca tcatgtacct gaacctcgtc ccccagaaga tgtgaggcag
     3001 gcgatggcga ccgctggccc cgtgatccgc atcggccgcc gcatcggcgg gccgttcgag
     3061 acggtctggg accagttccg cttctacggc gaggtgctgc gcgggctgcc cacgtcgttc
     3121 aggtacggca gcgtcatcct gcggctcatc accgacgtca cggtcggcgc cggcgcgctg
     3181 atcctgggcg gcggcatgtt cttcgtgatc gcctcgctgg ccttcttcac gggcgcggtg
     3241 gtcggcttcg aggggtacgt gggcctggag cagatcggcg cgcaggccta cgtgggcctg
     3301 gtgtccagtt gggcgaacac gcgcgagatc accccgctca tcgccggtgt cgccctggcc
     3361 gcccaggtgg gcgccggcta caccgccgag ctcggcgcca tgcgcatctc cgaggagatc
     3421 gacgcgctgg aggtcatgag cgtcccgagc atcgtctacc tggtgtccac ccgcgtgtgg
     3481 gcggccctga tcccgatcgt gccgctgtac ctcatggcgc tgttctccag ctactacgcc
     3541 acgcgcatga ccgtcaccgg ctacttcggc ctgtccaccg gctcctacgg gcagtacttc
     3601 aacctgttcc tgcccgccac ggacgtgctg tacagcgtca ccaaggtgat cgtgttcgcc
     3661 atcgtggtgg tgctcatcca ctgctactac ggctactacg cctcgggcgg gccggcgggt
     3721 gtgggcgtgg cggtcgggcg cgccatccgc accagcatcg tcgcggtggt cgtgctgaac
     3781 ctgctgctgt cgatcctgat gtggggcggc ggcgacacgg t
//
LOCUS       DATMKC010000347         2021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_134967, whole genome shotgun sequence.
ACCESSION   DATMKC010000347 DATMKC010000000
VERSION     DATMKC010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134967"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VNU01_10185"
     CDS             complement(<1..1809)
                     /locus_tag="VNU01_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH33027.1"
                     /translation="MKKRVRLAAASMAAVLSLPAALPASVTAAAAAATAAVIVQAPGA
                     EARVAAAVRDLGGTVTRALPIVGGFAAVLPEAALPRLAAEPGVTARTPDGRMVPQATK
                     TGDGTNLSTLKSVYREEVGADVLNAAGFTGAGVTIALVDTGIDTEVAGSGDLKGKVLA
                     VDDPMVPPTAAQPDPPDVECVDFSGEDTCDDTFGHGTFMAGLMAGSGAASGGSYKGVA
                     PDARLVSIKVGGRDGSADVSKVLAGIQWAVSFKDRYDIDVLNLSLGSDSVQPPEIDPL
                     NLAVQRAWVSGLTVVVSAGNFGAGPRTRPDGTTYGTVTKPGDDPFVITVGSSDDRETP
                     GIDDNRLPHFSSWGPTFHGLAKPDVIAPGGRVISLRAPGSFIDETIHAGKLDAAHRRG
                     SGTSMSAAVTSGLAALLLDARDGSDGLPEWRPDDVKAALIAGARKIKVNEPRAIGAGQ
                     VHGPSALAASISPTYRISVLSDGGGTLDASRGTNRVAVPCPEPILSAEPQPECWQTLE
                     GEQTAQGNNWQETEALGNNWQGNNWQGMEFADSEWTPQSWYESQWVSTVGNNWQGNNW
                     QATTWANGNNWQGNNWQGSTWNGQTDGTSYGQTTRGS"
BASE COUNT          248 a          723 c          745 g          305 t
ORIGIN      
        1 ggagcccctc gtcgtctggc cgtaggacgt gccgtcggtc tgcccgttcc aggtcgagcc
       61 ctgccagttg ttgccctgcc agttgttgcc gttggcccag gtggtggcct gccagttgtt
      121 gccctgccag ttgttgccca ccgtgctgac ccactggctc tcgtaccagc tctgcggcgt
      181 ccactcggag tcggcgaact ccatcccctg ccagttgttg ccctgccagt tgttgccgag
      241 cgcctcggtc tcctgccagt tgttgccctg tgcggtctgc tcgccctcca gggtctgcca
      301 gcattccggc tgcggctccg cggacaggat cggctcaggg cacggcacgg cgacgcggtt
      361 ggtgccgcgg ctggcgtcca gggtgccgcc gccgtccgac agcacggaga tgcggtaggt
      421 gggcgagatc gacgcggcca gcgcgctggg cccgtgcacc tggccggcgc cgatcgcgcg
      481 cggctcgttc accttgatct tgcgcgcgcc ggcgatcagc gccgccttga cgtcgtcggg
      541 ccgccactcg ggcaggccgt cggagccgtc ccgcgcgtcg agcagcagcg ccgccaggcc
      601 gctggtgacc gccgcggaca tggaggtgcc gctgccgcgc cggtgcgccg cgtccagctt
      661 gccggcgtgg atggtctcgt cgatgaagct gccgggcgcg cgcagggaga tcacgcgccc
      721 gccgggtgcg atgacatcgg gcttcgccag gccgtggaag gtcgggcccc acgaggagaa
      781 gtgcggcagg cggttgtcgt cgataccggg ggtctcgcgg tcgtcggagg agccgaccgt
      841 gatgacgaac gggtcgtcgc ccggcttggt gaccgtgccg taggtggtgc cgtccggacg
      901 cgtgcgcggg ccggcaccga agttgccggc ggagacgacc acggtcaggc cggacaccca
      961 cgcgcgctgc acggccaggt tcagcgggtc gatctccggc ggctgcacgc tgtcgctgcc
     1021 cagcgacagg ttgaggacat cgatgtcgta gcggtccttg aacgacaccg cccactggat
     1081 gcccgcgagc accttggaga cgtccgcgga cccgtcgcgg ccgccgacct tgatggacac
     1141 gagcctggcg tccggcgcca cacccttgta gctgccgccg gacgcggcgc cgctgcccgc
     1201 catcagcccg gccatgaacg tgccgtggcc gaaggtgtcg tcgcaggtgt cctcaccgga
     1261 gaagtcgacg cactcgacgt ccggcgggtc cggctgcgcg gcggtgggcg ggaccatcgg
     1321 gtcgtcgacc gcgagcacct tgcccttcag gtcgccgctg ccggcgacct ccgtgtcgat
     1381 gccggtgtcg accagcgcga tcgtgacgcc ggcgccggtg aacccggcgg cgttgagcac
     1441 gtcggcgccg acctcctcac ggtagaccga cttgagcgtc gacaggttgg tgccgtcgcc
     1501 ggtcttggtc gcctgcggca ccatgcgccc gtccggcgtg cgcgcggtca cgccgggctc
     1561 ggccgccagg cgcggcagcg ccgcctcggg caggaccgcc gcgaatccgc cgacgatggg
     1621 cagggcgcgc gtgaccgtgc cgccgaggtc ccgcaccgcc gccgcgacgc gcgcctcggc
     1681 gcccggcgcc tggacgatga cggccgcggt ggcggcggcc gcggcggccg tcaccgatgc
     1741 gggcagcgcc gcgggaaggc tcagcaccgc cgccatggag gcggcggcca atctgacacg
     1801 cttcttcacg atttgccctc cacctgtcga cgtacagacc gtcgcgagcc cgccacagtg
     1861 cgtgaatcag cgcggcgcgc gcgcacgcgc cgcgcagaag aagatgattc cccgggttgg
     1921 ggggccaaaa cgcaccctgg cgccatttct gccgacgggt gaaccgggtc ggcgcgtgga
     1981 atgctgaggc ttcggcgagc atgatcgcgg gccggcgcgc a
//
LOCUS       DATMKC010000348         5297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_318239, whole genome shotgun sequence.
ACCESSION   DATMKC010000348 DATMKC010000000
VERSION     DATMKC010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318239"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VNU01_10190"
     CDS             <1..1238
                     /locus_tag="VNU01_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWH33028.1"
                     /translation="FRSVSADGGANAILFDPANGSDGASLSVTGSTADGSGGVIRASS
                     GDAVVVGGGSVSLARMRVEDGAGRAIAADGAASVSLTRVTLSGNGGAGGLPTVDLAET
                     TGAVTIMSSTLSAGSGDLLRVAASATTPTVTVYQSTFSDGDGAGLLVEADADGAVSAT
                     VSGSTFDGLAGPGVRWRAGLSPGADGHSLLTVVSSAFRDRAGAGGSHLALELGASSAT
                     TAVLGAAGQPNTFDTVGGTGAAAIQLDVSGGAVLEATVAASTIDAPRGRGLALEAAQA
                     ADVTLLVDANTIRDAVAEALWAAAGAGDGDSAVDVTVTANQVSGANAGAAALAAIAVE
                     SAEHTLCADVSGNTVTATAGGAFDVVLRAVDPGLLRYAMAGSGTVTDADVVARNTIGA
                     AQVTGAGVSGSGGAACRLP"
     gene            complement(1245..3803)
                     /locus_tag="VNU01_10195"
     CDS             complement(1245..3803)
                     /locus_tag="VNU01_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWH33029.1"
                     /translation="MTTTVSGARRLVLAPLRGLSGAQRVWLLTAAMSAATAALVAGPL
                     SALPAPATGLGVPWPLLAVLFALCEVVVVNVQFRRDAHTISLGELPLVVGLYFVSPVG
                     LVAAQLTGAAVALALHRRQTPMKLAFNVAHFGLEAALAALLFSLVPAHALFGAAGWAA
                     VFAATTFAAVLAVSAMFAAVSLSEGRPAFAGWGREISTGLMFTGATTSMALLAVWMLA
                     QRPQALWLLAVPGATLWTAYRAWVRERERGERLRFLSEVTQAISAAPDLEGAVAALLS
                     HARGMFRAEVAELTLYPAEGSGLPLRTTAGPEGHGQVLRPVPHAGPRPARGAAALFRA
                     GEPVPEGLAGRGYRDAMTATLATERGVFGELVVADRTCEATPFDQEDLQLLGTLAAQA
                     SVCLENGRLEKTLVQLSALKEHLRHQAFHDSLTGLVNRALFHDRVEHALSSRRREDGA
                     VIVLLLDLDDFKAVNDSLGHDAGDQLLVEVAGRLEGCVRPGDTAARLGGDEFAVLVDD
                     VEGIGAGIVVAERIHAALRAPVVLHGMEVAVHASIGIARADGIDDAATVLRNADVAMY
                     EAKQQGKNRYGIYEHGMHHSARERLELRTQLRAAVDEDQLLLRFQPVVDLVSGAVVGA
                     EALVRWAHPTRGLLAPDAFIAYAEESGLIVPLGAWVLDGALAQLSRWRADLGTSRPFT
                     LAVNLSPRQLTQPGLAEEVRRLLGLHGVPPSDLMLEITESALIKDTPATLRVLRDLKA
                     LGVRIAVDDFGTGYSALSYLRQFPIDVLKISKPFVDDVRRGAEERALTEAIVRIAQAL
                     RLDTIAEGVEHADQREALRDMGCGYGQGFHFARPLPAGDLGALLRGPAAVTRSA"
     gene            complement(3835..>5297)
                     /locus_tag="VNU01_10200"
     CDS             complement(3835..>5297)
                     /locus_tag="VNU01_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH33030.1"
                     /translation="VELPRADHPHQGVQGVRAAVPDRPVRLRHEVDGYDPSADPHALH
                     ALVRVIGARQFYDEGWTGAGVDVALIDSGVAAVAGLDQPGGLLHGPDLSFESQSDALR
                     HVDTFGHGTHMAGIIAGRDAGVTVPRAADHHAFHGVAPGARVVSLKVADRNGATDVSQ
                     VIAAIDWVVQHRDRAGLRIRVLNLSFGTDAVQDYRIDPLAHAAEVAWRNGIVVVAAAG
                     NAGTGSPALDNPALDPFLLAVGAQDTRGTQGLGDDVVPAFSSTGDGVRNPDLVAPGVS
                     VASLRVPDSGIDRHHPGGRVGSRYFRGSGTSQAAAVVSGAVALLLQRHPGLTPDEVKQ
                     HLRASAEPLPAADPRGQGSGALDLEEALRRPPQLGARQPWEPSSGAGSLDGARGSLRL
                     SHDDVVLDGEQDIFGAPFDAAAHARLAEAGTAWSDGDWNGGPWTGAGWLGDRWAATVW
                     SRSSWSRSSWSSGGWTRSSWTRSSWSGDAWAGGGWG"
BASE COUNT          677 a         2063 c         1978 g          579 t
ORIGIN      
        1 cgttccgctc ggtcagcgcc gacggcggcg cgaacgcgat cctgttcgac cccgccaacg
       61 gcagcgatgg cgcctcgctg tccgttacgg gatcgaccgc ggacggcagc ggcggcgtca
      121 tccgcgcgag cagcggcgac gccgtggtgg tcggcggcgg cagcgtgtcg ctggcgcgca
      181 tgcgcgtcga ggacggcgcg ggccgcgcca tcgccgccga cggcgcggcg agcgtgtcgc
      241 tgacccgcgt gacgctgagc ggcaacggcg gcgccggcgg cctgcccacg gtcgacctcg
      301 ccgagaccac cggcgcggtc accatcatgt ccagcacgct gagcgccggc agcggcgacc
      361 tgctgcgcgt cgccgccagc gccacgaccc cgaccgtcac cgtgtaccaa tcgaccttca
      421 gcgacggcga cggcgcgggg ctcctggtgg aggccgacgc ggacggcgcc gtgagcgcca
      481 ccgtgagcgg cagcacgttc gacggcctcg ccggcccggg cgtgcgctgg cgcgccggcc
      541 tgtcccccgg cgccgacggg cactcgctgc tcaccgtggt gtccagcgcg ttccgcgacc
      601 gcgcgggcgc cggcggcagc cacctcgcgc tggagctcgg cgcctccagc gccaccaccg
      661 ccgtgctggg cgcggccggc cagccgaaca ccttcgacac ggtcggcggg acgggggccg
      721 cggccatcca gctcgacgtg tccggcggcg ccgtgctgga ggccaccgtg gcggccagca
      781 ccatcgacgc gccgcgcggg cgcgggctcg cgctcgaggc ggcgcaggcc gccgacgtga
      841 ccctgctggt ggacgccaac accatccgcg acgccgtcgc ggaggcgctg tgggcggcgg
      901 ccggcgccgg cgacggcgac agcgcggtgg acgtcacggt gaccgccaac caggtgagcg
      961 gggcgaacgc cggcgcggcg gccctggcgg ccatcgccgt ggagagcgcc gagcacacgc
     1021 tgtgcgccga cgtgagcggc aacacggtga ccgccacggc gggcggcgcg ttcgacgtcg
     1081 tgctgcgcgc ggtcgacccg ggcctgctgc gctacgcgat ggcgggcagc ggcacggtga
     1141 cggacgcgga cgtggtggcg cgcaacacga tcggcgcggc acaggtcacg ggagccggcg
     1201 tgagcggttc cggcggcgcg gcctgccggc tcccgtgacg cgcgtcacgc ggagcgcgtc
     1261 accgccgccg gcccgcgcag cagcgcgccc aggtcaccgg cgggcagcgg ccgcgcgaag
     1321 tggaagccct gcccgtagcc gcagcccatg tcgcgcagcg cctcgcgctg gtcggcgtgc
     1381 tcgacgccct cggcgatggt gtccaggcgc agcgcctgcg cgatgcgcac gatcgcctcg
     1441 gtgagcgcgc gctcctccgc gccgcggcgc acgtcgtcga cgaacggctt ggagatcttc
     1501 agcacgtcga tggggaactg ccgcaggtag gacagcgcgg agtagccggt gccgaagtcg
     1561 tccaccgcga tgcgcacgcc cagcgccttg aggtcgcgca gcacgcgcag cgtggccggc
     1621 gtgtccttga tgagcgcgct ctcggtgatc tccagcatca ggtcggacgg cggcacgccg
     1681 tgcaggccca gcagccggcg cacctcctcg gccagcccag gctgcgtgag ctggcgcggc
     1741 gacaggttga ccgccagggt gaacggccgc gacgtgccca ggtcggcgcg ccagcgcgac
     1801 agctgcgcca gcgcgccgtc gagcacccac gcgcccagcg gcacgatcag gccgctctcc
     1861 tccgcgtagg cgatgaaggc gtcgggggcg agcagcccgc gtgtgggatg ggcccagcgc
     1921 accagcgcct ccgcgcccac cacggcgccg gagaccaggt ccaccaccgg ctggaagcgc
     1981 agcagcaact ggtcctcgtc caccgcggcg cgcagttgcg tgcgcagctc cagccgctcg
     2041 cgcgcgctgt ggtgcatgcc gtgctcgtag atgccgtagc ggttcttgcc ctgctgcttg
     2101 gcctcgtaca tcgccacgtc ggcgttgcgc agcacggtgg cggcgtcgtc gatcccgtcg
     2161 gcgcgggcga tgccgatgct ggcgtggacg gcgacctcca tgccgtgcag caccacgggc
     2221 gcgcgcagcg cggcgtggat gcgctcggcc accacgatcc ccgcgccgat gccctccacg
     2281 tcgtcgacca ggacggcgaa ctcgtcgccg cccagccgcg cggcggtgtc gccggggcgc
     2341 acgcagcctt ccagccgccc ggccacctcc acgaggagct ggtccccggc gtcgtggccg
     2401 aggctgtcgt tgaccgcctt gaagtcgtcc aggtccagca gcagcacgat gaccgcgccg
     2461 tcctccctgc gccgcgagga cagcgcgtgc tccacgcggt cgtggaacag cgcgcggttg
     2521 accagcccgg tcagcgagtc gtggaacgcc tgatggcgca ggtgctcctt gagcgccgac
     2581 agctgcacca gggtcttctc caggcgcccg ttctccaggc acacgctggc ctgcgccgcg
     2641 agcgtgccga gcagctgcag gtcctcctgg tcgaacggcg tcgcctcgca agtgcggtcg
     2701 gccaccacca gctcgccgaa caccccgcgc tccgtggcca gggtggccgt catggcgtcg
     2761 cggtagccgc ggccggccag cccctcgggc accggctcgc cggcgcggaa cagcgcggcc
     2821 gcgccgcggg ccggccgcgg accggcgtgc ggcaccggcc gcagcacctg cccgtggcct
     2881 tccggccccg cggtggtgcg cagcggcagg ccgctgccct cggccgggta cagggtgagc
     2941 tcggcgacct cggcgcggaa catgccgcgc gcgtgggaca gcagcgccgc gaccgcgcct
     3001 tccaggtccg gtgcggcgct gatggcctgg gtgacctccg acaggaagcg cagccgctcg
     3061 ccgcgctcgc gctcgcggac ccacgcgcgg taggcggtcc acagcgtggc gccgggcacc
     3121 gcgagcagcc acagcgcctg cggccgctgc gccagcatcc acaccgccag cagcgccatg
     3181 ctcgtggtgg cgccggtgaa catcaggccg gtggagatct cccgtcccca cccggcgaag
     3241 gctggccggc cctcgctcag cgacacggcg gcgaacatcg cgctgaccgc cagcaccgcc
     3301 gcgaacgtgg tggcggcgaa cacggccgcc cacccggccg cgccgaacag cgcgtgcgcc
     3361 ggcaccagcg agaacagcag cgcggccagc gccgcctcca ggccgaagtg cgccacgttg
     3421 aacgcgagct tcatgggcgt ctggcggcgg tgcagcgcca aggcgacggc ggcgccggtg
     3481 agctgcgcgg cgaccagccc caccggcgag acgaagtaca ggcccaccac cagcggcagc
     3541 tcgccgaggg agatggtgtg cgcgtcacgg cggaactgca cgttcaccac cacgacctcg
     3601 cacagggcga acaggaccgc cagcagcggc cacggcacac ccagccccgt ggccggcgcg
     3661 ggcagggcgg acagcggacc ggccaccagg gccgccgtgg cggccgacat cgccgccgtg
     3721 agcagccaca cgcgctgcgc gccggacagg ccacgcagcg gcgcgaggac cagccgcctc
     3781 gcgccgctca ccgtcgtcgt catcccgacc acctccccag ctcgccgggg ccgctcagcc
     3841 ccagcctccc ccggcccagg cgtccccgct ccacgagctg cgggtccagc tcgagcgggt
     3901 ccagccaccg gacgaccagc tcgaccggct ccacgagctg cgcgaccaca ccgtggccgc
     3961 ccagcggtcg ccgagccagc cggcgccggt ccacggaccg ccgttccagt ccccgtcgct
     4021 ccacgccgtc ccggcctcgg ccagtcgcgc gtgggcggcg gcgtcgaacg gcgccccgaa
     4081 gatgtcctgc tccccgtcca gcaccacgtc gtcgtgggac aggcgcagcg agccgcgcgc
     4141 gccgtccagc gagccggccc ccgacgacgg ctcccacggc tggcgcgcgc cgagttgcgg
     4201 ggggcgccgc agcgcctcct ccaggtcgag cgcgccgctg ccctggccgc gcgggtccgc
     4261 cgccgggagc ggctcggcgc tggcgcgcag gtgctgcttg acctcgtcgg gcgtcaggcc
     4321 ggggtggcgc tgcaggagca gcgccaccgc gccggagacc acggcggcgg cctgcgacgt
     4381 gccgctgccg cggaagtagc gcgaccccac gcgcccgccg gggtgatgcc ggtcgatgcc
     4441 gctgtccggc acgcgcaggc tggccacgga cacgccgggc gccaccaggt cggggttgcg
     4501 caccccgtcg ccggtgctgg agaacgcggg caccacgtcg tcgcccaggc cctgggtgcc
     4561 ccgcgtgtcc tgcgcgccga ccgccagcag gaacgggtcc agcgccgggt tgtccagcgc
     4621 cggcgagccg gtgccggcgt tgccggccgc ggccaccacc acgatgccgt tgcgccaggc
     4681 cacctcggcg gcgtgcgcca aggggtcgat gcggtagtcc tgcaccgcgt cggtgccgaa
     4741 ggacaggttg agcacgcgga tgcgcaggcc ggcgcgatca cggtgctgca ccacccagtc
     4801 gatcgcggcg atcacctggg acacgtccgt ggcgccgttg cggtcggcca ccttcaggct
     4861 caccacgcgc gcgccgggcg cgacgccgtg gaaggcgtgg tggtcggcgg cgcgcggcac
     4921 ggtcacgccg gcgtcgcgcc cggcgatgat cccggccatg tgggtgccgt gcccgaacgt
     4981 gtcgacgtgg cgcagcgcgt cggactggga ctcgaacgac aggtccggcc cgtgcagcag
     5041 cccgccgggc tgatccaggc ccgccacggc ggcgacaccc gagtcgatca gggccacgtc
     5101 gacgcccgcg ccggtccagc cctcgtcgta gaactgccgc gcgccgatca cccgcaccag
     5161 ggcgtgcagg gcgtgcgggt cggcggacgg gtcgtagccg tccacctcgt ggcgcagacg
     5221 caccggccgg tccgggaccg cggcgcgcac gccctgcacg ccctggtgcg ggtgatcggc
     5281 gcgcggcagt tctacga
//
LOCUS       DATMKC010000349         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_299679, whole genome shotgun sequence.
ACCESSION   DATMKC010000349 DATMKC010000000
VERSION     DATMKC010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299679"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VNU01_10205"
     CDS             <1..841
                     /locus_tag="VNU01_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWH33031.1"
                     /translation="LDLEDSVAPNVKEEARGNVIEALRTNDWGNKTVVVRINAIDTRW
                     AYRDILEVVEAAGEHIDCLMIPKVQSPGEVEFVDHMLRMIEETKGFEHRIGLEAQIEN
                     GPGLTLIDDIAFASDRLETLIFGPGDMAAALGMPSLTVGESIADYPGDHWHWVLMRIL
                     VAARAAGLQAIDGPFAKIRDHDGYREIAKRSKSLGYDGKWVLHPDQITIANEVFSPTQ
                     EEFERAEDILTAYEKATDVDRTGAVMFGDEMIDEASRKMAEVTSMRGRAAGMTVRPES
                     ERG"
     gene            complement(838..2040)
                     /locus_tag="VNU01_10210"
     CDS             complement(838..2040)
                     /locus_tag="VNU01_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWH33032.1"
                     /translation="MLVAATSANSALLLAELGLVVVGLALLARAAAWAGFSPIPLYLL
                     GGLALGRGGIVPLGLSEDFIGVGAEIGVVLLLFMLGLEYTAAELAGSLRTGLRAGGED
                     LLLNFTPGVLFALAWGWSPVTAVLLGGVTYISSSGVVAKVLGDLGRMGNRETPAVLTI
                     LVVEDLAMALYLPVVAVLLAGTGWTTGALSLGLAVATVVVALIVALRFGGAVSRVLFS
                     TSDEVLLLTTLGTVLLVGGLAQRLQVSAAVGAFLVGLALSGPVAERARELLGPLRDLF
                     AATFFVFFGLQIDPGDLPPVLAAAALLALVTGATKILTGWRAARALGVRAPGRLRAGT
                     VLAARGEFSIVIAGLGVASGVEEALGPLAAAYVLILAVGAPILARIADPLAAAVAGRG
                     SGRDAPVS"
BASE COUNT          366 a          795 c          762 g          215 t
ORIGIN      
        1 cctggacctc gaggactccg tcgcgccgaa cgtgaaggaa gaggcgcgcg gcaacgtcat
       61 cgaggcgctg cgcaccaacg actggggcaa caagaccgtc gtcgtgcgca tcaacgcgat
      121 cgacacgcgc tgggcctacc gggacatcct ggaggtcgtc gaggccgccg gcgagcacat
      181 cgactgcctg atgatcccca aggtccagtc gccgggtgag gtcgagttcg tcgaccacat
      241 gctgcgcatg atcgaggaga ccaagggctt cgagcaccgc atcggcctgg aggcccagat
      301 cgagaacggc ccgggcctga cgctgatcga cgacatcgcg ttcgcgtcgg accgcctgga
      361 gacgctgatc ttcggccccg gcgacatggc cgcggcgctg ggcatgccgt cgctgaccgt
      421 gggcgagtcc atcgccgact accccggcga ccactggcac tgggtgctga tgcgcatcct
      481 ggtcgccgcg cgcgccgcgg gccttcaggc catcgacggg ccgttcgcca agatccgcga
      541 ccacgacggc taccgcgaga tcgccaagcg gtccaagtcg ctcggctacg acggcaagtg
      601 ggtcctgcac ccggaccaga tcaccatcgc caacgaggtg ttctcgccga cgcaggagga
      661 gttcgagcgg gccgaggaca tcctcaccgc ctacgagaag gccaccgacg tcgaccgcac
      721 gggcgccgtg atgttcggcg acgagatgat cgacgaggcc tcccgcaaga tggccgaggt
      781 cacgtccatg cgcggccgcg ccgccggcat gaccgtgcgc ccggagtccg agcgcggcta
      841 agacaccggc gcgtctctcc cggaccccct cccggccacc gcggcggcga gggggtccgc
      901 gattctggcg agaatcgggg cgccgacggc caggatgagc acgtaggcgg cggcgagcgg
      961 gccgagcgcc tcctccaccc cggaggccac gcccaggccg gcgatgacga tggagaactc
     1021 gccacgagcg gccaggaccg tgccggcgcg caggcggccg ggcgcgcgca cgcccagggc
     1081 ccgcgccgcc cgccagccgg tgaggatctt ggtggcgccg gtgaccagcg ccagcagcgc
     1141 ggctgcggcg agcaccggcg gcaggtcgcc cgggtcgatc tgcaggccga agaacacgaa
     1201 gaaggtcgcg gcgaacaggt cgcgcagcgg gccgagcagc tccctggcgc gctccgccac
     1261 ggggcccgac agcgccaggc ccaccaggaa ggcgccgacg gcggcggaga cctgcaggcg
     1321 ctgcgccagc ccgccgacga gcagcacggt gccgagcgtc gtcagcagca gcacctcgtc
     1381 ggaggtggag aacagcacgc ggctcaccgc gccgccgaag cgcagcgcca cgatcagggc
     1441 cacgaccacg gtggcgaccg ccagtcccag cgacagcgca cccgtggtcc agccggtgcc
     1501 ggcgagcagc accgccacca cgggcaggta cagcgccatc gccaggtcct ccaccaccag
     1561 gatggtcagc accgccgggg tctcgcggtt gcccatccgg cccaggtcgc ccagcacctt
     1621 ggcgaccacg ccggaggacg agatgtacgt gacgccgccc agcagcacgg ccgtcaccgg
     1681 cgaccagccc caggccagcg cgaacagcac gcccggcgtg aagttcagca ggaggtcctc
     1741 gccgccggcg cgcaggccgg tgcgcagcga gccggccagt tcggcggcgg tgtactccag
     1801 cccgagcatg aacagcagca gcaccacgcc gatctcggcg cccaccccga tgaagtcctc
     1861 cgacaggccc agcggcacga tgccgccgcg gccgagcgcg agcccgccga gcaggtacag
     1921 cgggatcggc gagaagccgg cccacgcggc cgcgcgggcc agcagcgcca ggcccaccac
     1981 cacgaggccg agctccgcga gcagcagggc gctgttcgcg gacgttgcgg cgacgagcac
     2041 ctagccggcc ctcagcacgt cgagagcgcg ggcgatgccc tccggcgtcc cgaccagcac
     2101 ggcgacgtcg ccgtgctggt cgggacgccg gagggcat
//
LOCUS       DATMKC010000350         3157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_55167, whole genome shotgun sequence.
ACCESSION   DATMKC010000350 DATMKC010000000
VERSION     DATMKC010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55167"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VNU01_10215"
     CDS             complement(<1..1037)
                     /locus_tag="VNU01_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWH33033.1"
                     /translation="MRRRAGLPAAGLALAALFAAPLLYLSGWAAGDPPGVLRLWGSAA
                     TLAPLGRTLLLAVTVSLATATLGTALAWLTARTDLPARRLWRLLLPLPLVIPSFIGAF
                     SLIAAFAPGGLLDQLLTPLGARELPQVRGYWGAFAVLTLLTYPYVYLPVAARLRQLPP
                     SLEESARLLGHSPLSAFRTTVLPQTRGAVLAGSLLVFLYTVSEFGAVQLMRYDTLTRS
                     IYASRIYEQRRALALSLLLGVLAVGIAAAERWLGGLREHRTARGGRALEVPLGAWRRP
                     ALALVAGVALFALVAPLAVLLFWAVRGVLRGDTAGGALAADIAGLLSPALHTATVSMA
                     AAAHRQVRPGGP"
     gene            complement(1034..2035)
                     /locus_tag="VNU01_10220"
     CDS             complement(1034..2035)
                     /locus_tag="VNU01_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWH33034.1"
                     /translation="MTRRTVAALLALALAAALTACSGGREALTIYSGRTENLVGPLLE
                     RFHAETGIPIDVKYGDSAELALLLDTEGERTSADVFLSQTPGANAFLAGKDLLLELPA
                     EVVERVDERFRDEHGVWVGVTGRQRVLVYNRELVEPSALPTSVDQLTQPQFAGKVAVA
                     PTNGSFQDFVTAMRQVRGDDETLTWLRALDAGGAPTYANNNAIVDAVARGEVAMGLVN
                     HYYNHRFLDEDPSLPSRNHQFPGDDIGSLLIPSTVSVLASTDQRDNALRFVEFLLEAQ
                     AQRYFSEETFEYPLGEGAEPADTLPPLETLEAFPYRFAELGGGLERTVELIRTSGLA"
     gene            complement(2032..2991)
                     /locus_tag="VNU01_10225"
     CDS             complement(2032..2991)
                     /locus_tag="VNU01_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH33035.1"
                     /translation="MAIVVTGAAGFIGAQLVARLLRDGHAVVGVDRRGGIPPGATPLL
                     TDLSRPDDAVRAALAEAEAVFHLAGCPGVRDRSPDVAARRLRDNVLAGERVLAATAPG
                     TPLVVASSSSVYGGARRGPHGAFRPCAERDRPAPRGGYARSKLVLEERCARRAAAGGH
                     VVVLRPFTVAGEGQRPDMALSLWIDAARDGRPLRVLGSPDRRRDVTDVRDVVEGMVRA
                     ARREATGVVNLGTGVGHRLADLADAVRRAVGADVPLAVEAVSPVEPPATLADTRRCRR
                     VLGFTPATDLVSLVRRQAAAQLGAPPEPAAAAHPDPHAVLETA"
BASE COUNT          424 a         1175 c         1230 g          328 t
ORIGIN      
        1 gggccgccgg ggcgtacctg acggtgcgcc gcggcggcca tgctcaccgt ggccgtgtgc
       61 agcgcgggcg acagcaggcc cgcgatgtcc gccgccaggg cgccgccggc ggtgtcgccg
      121 cgcagcacgc cgcgtaccgc ccagaacagc aggaccgcga gtggggcgac cagcgcgaac
      181 agggcaacgc cggcgaccag cgcgagcgcg ggccggcgcc atgcgcccag gggtacctcc
      241 agcgcccggc cgccgcgggc ggtgcggtgc tcgcgcagcc cgcccagcca gcgctccgcc
      301 gcggcgatgc ccacggccag gacgccgagc agcagcgaca gcgccagggc gcggcgctgc
      361 tcgtagatgc gcgaggcgta gatgctgcgc gtcagcgtgt cgtagcgcat cagctgcacg
      421 gcgccgaact cgctcaccgt gtacaggaac accagcagcg acccggccag caccgcgccg
      481 cgcgtctgcg gcagcacggt ggtgcggaac gccgacagtg gcgagtgccc cagcaggcgg
      541 gcgctctcct ccagcgacgg cgggagctgg cgcagccgcg cggcgaccgg gaggtacacg
      601 tagggatagg tcagcagggt gagcaccgcg aacgcgcccc agtagccccg cacctgcggc
      661 agttcgcgcg cccccagggg cgtgagcagc tggtcgagca ggccccccgg cgcgaacgcg
      721 gcgatcagcg agaacgcgcc gatgaagctc gggatgacca gcggcagcgg cagcagcagg
      781 cgccacagcc gccgcgccgg caggtccgtc cgcgcggtca gccacgccag cgccgtgccg
      841 agcgtggcgg tcgccaggga cacggtcacg gccagcagca gcgtgcggcc cagcggcgcg
      901 agcgtcgccg cggaccccca caggcgcagg acgccggggg ggtcacccgc ggcccacccg
      961 gacaggtaca gcagcggcgc cgcgaacagc gccgccaggg ccagcccggc cgccgggagc
     1021 cccgccctgc gcctcacgca agtccgctgg tgcggatgag ctcgaccgtg cgctccaggc
     1081 cgccgcccag ctcggcgaag cggtacggga acgcctccag cgtctccagc ggcggcagcg
     1141 tgtcggccgg ctcggcgccc tcgcccagcg ggtactcgaa ggtctcttcg gagaagtagc
     1201 gctgcgcctg cgcctccagc aggaactcca cgaagcgcag cgcgttgtcg cgctggtcgg
     1261 tggaggccag cacgctcacg gtcgagggga tcagcagcga gccgatgtcg tcgccgggga
     1321 actggtggtt gcggctgggc aggctggggt cctcgtccag gaagcggtgg ttgtagtagt
     1381 ggttcaccag ccccatggcg acctcgccgc gcgccaccgc gtcgacgatg gcgttgttgt
     1441 tggcgtaggt gggggcgccg ccggcgtcca gcgcgcgcag ccaggtgagg gtctcgtcgt
     1501 cgccccgcac ctggcgcatc gccgtcacga agtcctggaa ggagccgttg gtgggcgcga
     1561 ccgcgacctt gcccgcgaac tgcggctggg tgagctggtc caccgacgtg ggcagcgccg
     1621 acggctccac gagctcgcgg ttgtagacca gcacgcgctg gcggccggtg acgccgaccc
     1681 acacgccgtg ctcgtcgcgg aagcgctcgt ccacccgctc caccacctcg gcgggcagct
     1741 ccagcagcag gtccttgccc gccaggaacg cgttcgcgcc cggcgtctgc gacaggaaca
     1801 cgtccgcgct ggtgcgctcc ccctcggtgt ccagcagcag cgccagctcc gcggagtccc
     1861 cgtacttcac gtcgatgggg atccctgtct cggcatggaa gcgctccagc agcgggccga
     1921 cgaggttctc ggtgcggccc gagtagatgg tcaaggcctc gcgcccgccg gagcaggcgg
     1981 tcagcgcggc ggccagggcc agggcaagca gtgcggcgac ggtgcggcgg gtcatgcggt
     2041 ctccagaacg gcgtgagggt cggggtgcgc ggcggcggcc ggctccggcg gcgcgccgag
     2101 ctgggcggcg gcctggcggc gcaccagcga gacgagatcg gtcgcgggcg tgaagcccag
     2161 cacgcggcgg cagcggcgcg tgtcggccag ggtggcgggc ggctcgaccg gcgacaccgc
     2221 ctccacggcc agcggcacgt cggcgccgac cgcgcggcgc accgcgtcgg ccaggtcggc
     2281 cagccgatgg cccacgccgg tacccaggtt caccacgccg gtggcctccc ggcgggcggc
     2341 gcgcaccatg ccctccacga cgtcgcgcac gtcggtgacg tcgcggcgcc ggtcgggcga
     2401 gccgagcacg cgcaggggcc ggccgtcgcg cgccgcgtcg atccacagcg acagtgccat
     2461 gtccgggcgc tggccctccc cggcgacggt gaacgggcgc agcacgacga cgtggccgcc
     2521 ggcggccgcg cgacgggcgc agcgctcctc cagcaccagc ttggacctgg cgtagccgcc
     2581 gcgtggtgcc ggccggtcgc gttccgcgca gggccggaac gcgccgtggg ggccgcgccg
     2641 ggcgccgccg tagaccgacg aggaggacgc caccaccagc ggcgtgccgg gcgcggtggc
     2701 ggccagcaca cgctcgccgg ccagcacgtt gtcccgcagc ctgcgggcgg cgacgtccgg
     2761 ggagcggtcg cgcacgcccg ggcagccggc caggtggaac accgcctccg cttccgcgag
     2821 ggcggcgcgc accgcgtcgt ccggccgcga caggtccgtc agcagcggcg tggcgccggg
     2881 cgggatgccg ccgcggcggt cgacgccgac cacggcgtgg ccgtcgcgca ggagacgcgc
     2941 gacgagctgg gcgccgatga agccggcggc cccggtgacc acgatcgcca tgccaggggt
     3001 tccttgtgcg gtggtggcgg gccccgattg ggggccgcga gtgaagtgag gttaccctta
     3061 cctcatgagc gggagccaat cgagcgcggc ggcgcgccca acgccacccc acgggccgcg
     3121 catgcccggc ccttcacgcc tgcgccgctg ggcgggc
//
LOCUS       DATMKC010000351        10074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_295287, whole genome shotgun sequence.
ACCESSION   DATMKC010000351 DATMKC010000000
VERSION     DATMKC010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295287"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VNU01_10230"
     CDS             complement(<1..361)
                     /locus_tag="VNU01_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HWH33036.1"
                     /translation="MGSARLIVHGGLGTPDSPVVRQALEQALDAGWRELSQTGDPVAG
                     ACAAVRVLEDDGRFNAGSGGVPADTGEVELDAGVFQGWTGRFAGVAALRDVANPVLVA
                     AALLAHRPGAVLLAGEGA"
     gene            481..2814
                     /locus_tag="VNU01_10235"
     CDS             481..2814
                     /locus_tag="VNU01_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33037.1"
                     /translation="MSWRAFALRHLLGRLHIGEDILRHLTTFQRLGATLELAPPGEML
                     PVGARTALRAIRTRGAADISPDWLWPWWLQRQLDPTDDAFTPRGHLPVMQNVTHRNWT
                     LVGNMGSAWEAIVDPTGLVTAQFDGWSLDWWVRDADGRWHFPSRSAERVRQRLMDARP
                     VVETRLGLPDGEVVQRVYAVETVPELVVVEVHNAGARDCGLAMALRPYNPEGLAVVED
                     LSVHGQVVSVGKVPVLYLPRLPERSALSTFEDGDVVHVLEGRREAPPAREHVHDRAGL
                     AQGAFTFTLPAGETLRFAAPMAGRSPRRRWPMPWRRRMRHTVPQPERLPGAAQVRDEW
                     DRRMRRGTRVELPDARLQEAVDANRAFLLLLHDPGDITPGPMTYHRFWFRDAAYQLAA
                     LDRWGLHTEVADVLRTYPGRQRGDGFFYSQWREWDANGAAIWSLAEHERLTADAATRD
                     HTAAAVASGVEWILRTLDDPRGKEPEAAGLLPAGISAEHLGPFDYYYWDNLWAWRGLV
                     DGATLLHGSGRHDAAAAAARGAEALGARLRAAWDRDARRLGRRAVPAGPGRGFDAGMI
                     GALSAVYPLGLLAPDDEWMRGTAEVVRERFLLGQAFFQGISHTGLGTYLTVQLAMVEI
                     EAGDPRGWDRVEWLLDAATDTWTWPEAIHPRLPGGCMGDGHHGWMAADLLNVVRLTIA
                     RETADGLTLFSVLPERWRGGRVALRGAPTHHGLLDLELEWPDERPLLRWSLRRRDDRP
                     VRLTAPALDPSWHSDEPEGEARLGGVYADARSRRRAQ"
     gene            complement(2778..3437)
                     /locus_tag="VNU01_10240"
     CDS             complement(2778..3437)
                     /locus_tag="VNU01_10240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH33038.1"
                     /translation="MKAAAVIAAWNEAPTIAEVVGAARASVLVDEVIVVDGRSTDGTA
                     AGAEAAGARVLTQPEGGKGEAMAAGVAATDADVIVFLDGDLTGLRPHHVDRLVRTVTA
                     GGAVMACGLFDRGPGMNRLFLHLLPILTGERALRRELFESLAAADLAGYRIEAALNAR
                     AGELGLPVAAFVCQGMWHRTKEEKFSTPAQGWIAKVRMLLVAMGSYAGYWARRRLRAS
                     A"
     gene            complement(3434..3904)
                     /locus_tag="VNU01_10245"
     CDS             complement(3434..3904)
                     /locus_tag="VNU01_10245"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWH33039.1"
                     /translation="MRLRPTLLAGMGGALLAYLFDPQAGARRRHRMASQAAGTMRARR
                     RGMSKRGEYLAGRSKGMVRELRPDQQPENAQTLVDKVRSEVLGAEQWRHYTVNVSAAD
                     GVVMLNGQLDRPEQIERLVHEVRQVTGVRDVENALHLPGTLPPNKRSAMTARVR"
     gene            complement(4012..4356)
                     /locus_tag="VNU01_10250"
     CDS             complement(4012..4356)
                     /locus_tag="VNU01_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33040.1"
                     /translation="MAAFEQVGHFPSRPMAEVAATYLHANGIPATVWADDAGGTYPHV
                     ALYRGISLRVPAEDADAARALLAEADAAHALAPEPDAAERRPLRPVAKWVIRLAAAVV
                     VAGAAISALPPR"
     gene            4463..4963
                     /locus_tag="VNU01_10255"
     CDS             4463..4963
                     /locus_tag="VNU01_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33041.1"
                     /translation="MSGFEDKLRERLESNAALAEQRAAAEAEMDRAALQAQQEAAAAE
                     RELRASRDARHAELAARLEDLLDMLRGSGQRLEVRAGWSASGEEFYARLATHAGWPQR
                     VLSLELDRDDDEVLARWHSDVGNTLELWRLLEFDVEMLEELVLQVIDDELWRGASAPP
                     AFPEPA"
     gene            complement(4960..6534)
                     /locus_tag="VNU01_10260"
     CDS             complement(4960..6534)
                     /locus_tag="VNU01_10260"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWH33042.1"
                     /translation="MHHDDARPTSGPGGERPFGLRVLGRAGEPGWALRLRVQTLLTGA
                     VVVANLVGAAAVVALSVWLIPGPSLLDTRFGTVVVVAAPAYIVLAVAAGALSGTRREL
                     PRLRWVAEGGAPPGDGHSQVLRAPFRLLGLQVALWLGAVVLFGALGVPAGWRVVLRTT
                     TTVLLGGLVTCTCSYLLSELALRPLAARVLALPGAPERGAPGLAVRSLLVWLVGTGVP
                     VLGLMVVAVVALVEGDVPAGSLAITMLGLGGVTLAVSLLLVRLGIQGTLDPLRSMRRA
                     LAAVEAGRLDTRIVVYDASELGRLQAGFNRMVAGLEERERLRDLFGRHVGEPVATAAV
                     AGRFDAGGEVRDAAVVFVDIVGSTRLAAELPPERIVELLNRFFTVVVDVVDRHGGLVN
                     KFVGDAALAVFGVPTPLDDACGRALAAARELAGRLPAEVPECPAGIGVAAGRVVAGNV
                     GDQRRFEFTVIGDPVNEAARLTELAKSAPSRLLASAVALDAAGEAERGHWELVGEVTV
                     EGRATPTRLAAPRPPG"
     gene            6608..8701
                     /locus_tag="VNU01_10265"
     CDS             6608..8701
                     /locus_tag="VNU01_10265"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWH33043.1"
                     /translation="MNRLAEASSPYLLQHADNPVDWWEWGEDAFAEAKRRDVPVFLSV
                     GYSSCHWCHVMAHESFEDAEVAAYLNEHFVSVKVDREERPDVDAVYMGAVQAISGRGG
                     WPMSVFLTPDGTPFFGGTYWPKEERQGMPGFLRVIEAVSAAWTNERDKVVESGGKLAQ
                     HLREQQQVDASDERPDLTIPERAAELCVRMWDRRHGGFGQAPKFPQAMTIDFLLAHSL
                     RTGDAEAKEAATHSLTAMSKGGIYDHVAGGFARYSVDDVWLVPHFEKMLYDNALLLRA
                     YVHGWQATGNPRFRRIAVETAEYLLRDMRHARGAFYSATDADSEGEEGKFFLWHAEEF
                     FDTIQDGGENPEKWMAFYGVTPAGNFSDPHHPGSPMRSILYEALERDELDDEFAARAQ
                     KVREILYAKREQRVHPGLDDKILTSWNALAIGALAEAGAAFGIPRFVVAAQQAAAFLR
                     DEAQVHGRLMHVWKEGRGASVPAFLEDVAYLAQALLVLYEADFDPDWFRWALRLAQTA
                     WAEFGDEETGAFYSTAHDAEELLTRPKDLWDNATPAGSSVMVDVHLRLHALTGETQWL
                     ERAEKTIAAFQYRALQAPTGYGEFLRAMERMASGPMEVAIVGQLEDEATIRLIEVYRE
                     RWRPGAVAAVNGVVGGVEAADGAASTPDVHPVALLHERPLVGGRPAAYVCRQFACERP
                     VTSPDDLRDALDRRA"
     gene            8951..10057
                     /locus_tag="VNU01_10270"
     CDS             8951..10057
                     /locus_tag="VNU01_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWH33044.1"
                     /translation="MAAAQVLAVTGGVDTHAEVHVAAAIDAVGRELGHASFATTPAGY
                     AALLAWLESFGPLERVGVEGTGVYGAGLTRVLQAAGVRVIEVDRPDRKARRYQGKSDP
                     IDAYAAARAALSGRAAGTPKTRTGSVEMIRTLRVARAGAVKAKAVAWNQLKALIKTAP
                     EDLRERLRALDGAALLDACVALRAARGPAPEHGPHAKRHRPGVLADPTAACKRSLAVL
                     ARRVRALEAEITDLDNDLEPLIVATAPTLLGLYGVGLDVAGQLLATAGDNPDRLASEA
                     AFAHLCGVAPIPASSGKTERHRLNRGGDRHANNALWRIAMTRLRYDPATREYRDRRAK
                     EQKTNKDILRCLKRYIAREVHRALMADLTTPSPT"
BASE COUNT         1352 a         3722 c         3784 g         1216 t
ORIGIN      
        1 gcgccccctc ccccgccagc agcacggcgc cgggacgatg cgcgagcagc gccgcggcga
       61 ccagcaccgg gttggcgaca tcgcgcagcg cggccacgcc ggcgaagcgg ccggtccagc
      121 cctggaacac cccggcgtcg agctccacct cgcccgtgtc cgcgggcacc ccgccgctgc
      181 cggcgttgaa gcggccgtcg tcctccagga cgcgcacggc ggcgcacgcg cccgccacgg
      241 ggtcgccggt ctggctgagt tcgcgccagc ccgcgtccag cgcctgctcc agcgcctggc
      301 ggaccaccgg gctgtcgggc gtgccgagac caccgtgcac gatcaggcgc gcgcttccca
      361 tggcgcggcg gtacccgtcg cgaccgccgg ggaaaacgcc cggcggggcg gcgtgtcaac
      421 ggcggccgtc gcggtaaggg tggcggcgac cacgcccgcg accccgacgg aggagcccgc
      481 atgagctggc gcgcattcgc gctccgccac ctgctgggac ggctgcacat cggcgaggac
      541 atcctgcgcc acctgaccac gttccagcgc ctgggcgcga cgctggagct ggcgccgccc
      601 ggcgagatgc tgcccgtcgg cgcgcgcacg gccctgcggg ccatccgcac gcgcggcgcg
      661 gcggatatca gcccggactg gctgtggccg tggtggctcc agcgccagct cgatcccacc
      721 gacgacgcgt tcacgccgcg cggtcacctg cccgtgatgc agaacgtcac gcaccgcaac
      781 tggacgctgg tcggcaacat gggctcggcg tgggaggcga tcgtcgaccc caccggcctg
      841 gtcaccgcgc agttcgacgg ctggtcgctg gactggtggg tgcgcgacgc cgacggccgg
      901 tggcacttcc ccagccgctc cgccgagcgg gtgcgccagc gcctgatgga cgcgcggccc
      961 gtcgtggaga cccgcctggg gctgcccgac ggcgaggtcg tccagcgcgt gtacgccgtg
     1021 gagaccgtgc cggagctggt ggtcgtcgag gtccacaacg ccggcgcgcg cgactgcggg
     1081 ctggccatgg cgctgcgccc gtacaacccg gaggggctgg ccgtggtgga ggacctgtcc
     1141 gtgcacggcc aggtcgtctc ggtcggcaag gtccccgtcc tgtacctgcc gcgcctgccc
     1201 gagcgcagcg ccctgtcgac gttcgaggac ggcgacgtgg tgcacgtgct ggagggccgg
     1261 cgcgaggccc cgccggcgcg cgagcacgtg cacgaccggg ccggcctggc ccaaggcgcg
     1321 ttcacgttca cgctgccggc gggcgagacg ctgcgcttcg ccgcacccat ggcggggcgc
     1381 tcgccgcggc ggcgctggcc gatgccgtgg cggcggcgca tgcgccacac ggtgccgcag
     1441 ccggagcgcc ttccgggcgc cgcgcaggtg cgcgacgagt gggaccgccg catgcgccgc
     1501 ggcacgcggg tggagctgcc ggacgcgcgc ctgcaggagg cggtggacgc caaccgcgcg
     1561 ttcctgctgc tgctgcacga cccgggcgac atcacgccgg gaccgatgac ctaccaccgc
     1621 ttctggttcc gcgacgccgc ctaccagctc gcggcgctgg accgctgggg cctgcacacc
     1681 gaggtcgccg acgtcctgcg cacctacccg ggccgccagc ggggcgacgg cttcttctac
     1741 agccagtggc gcgagtggga cgccaacggc gccgcgatct ggagcctggc cgagcacgag
     1801 cggctcaccg ccgacgcggc cacgcgcgac cacacggcgg cggccgtcgc gtccggcgtg
     1861 gagtggatcc tgcgcacgct ggacgacccg cgcggcaagg agcccgaggc cgcgggcctg
     1921 ctgccggccg gcatctccgc cgagcacctg ggaccgttcg actactacta ctgggacaac
     1981 ctgtgggcgt ggcgcggcct ggttgacggc gccacgctgc tgcacggcag cgggcgccat
     2041 gacgcggcgg cggccgcggc gcgcggcgcg gaagcgctcg gcgcccggct gcgcgcggcg
     2101 tgggatcgcg acgcccgccg gctgggccgc cgcgcggttc cggccggccc ggggcgcggc
     2161 ttcgacgccg gcatgatcgg cgcgctcagc gcggtgtacc cgctgggcct gctggcgccc
     2221 gacgacgagt ggatgcgcgg caccgccgag gtggtgcgcg agcgcttcct gctgggccag
     2281 gcgttcttcc agggcatctc gcacacgggt ctcggcacgt acctgacggt gcagctcgcg
     2341 atggtcgaga tcgaggccgg cgacccgcgc ggctgggacc gcgtcgagtg gctgctggac
     2401 gccgcgaccg acacctggac gtggccggag gcgatccacc cgcggctgcc gggcggctgc
     2461 atgggcgacg gccaccacgg ctggatggcc gcggacctgc tcaacgtggt gcgcctgacc
     2521 atcgcgcgcg agaccgccga cggcctcacg ctgttcagcg tgctgccgga gcgctggcgc
     2581 ggcggccgcg tggcgctgcg cggcgcgccg acgcaccacg gcctgctgga cctggagctg
     2641 gagtggccgg acgagcggcc gctgctgcgc tggtcgctgc gccgccggga cgaccggccg
     2701 gtgcggctga ccgcgcccgc cctggacccg tcgtggcaca gcgacgagcc cgagggcgag
     2761 gcgcggctcg gcggggtcta cgcggacgcg cgcagccgcc gccgggccca gtagccggcg
     2821 tagctgccca tcgcgaccag cagcatgcgc accttggcga tccagccctg cgccggcgtg
     2881 gagaacttct cctccttggt gcggtgccac atgccctggc acacgaacgc ggcgaccggc
     2941 aggccgagct cgcccgcgcg cgcgttcagc gccgcctcga tgcggtagcc ggcgaggtct
     3001 gccgccgcca gcgactcgaa cagctcgcgg cgcagcgcgc gctccccggt gaggatgggc
     3061 agcaggtgca ggaacaggcg gttcatgccg gggccgcggt cgaacaggcc gcacgccatc
     3121 acggccccgc cggccgtcac ggtgcgcacc agccggtcca cgtggtgggg gcgcaggccg
     3181 gtgagatcgc cgtccaggaa cacgatcacg tcggcgtcgg tcgcggcgac gccggccgcc
     3241 atcgcctcgc ccttgccgcc ctccggctgg gtgagcaccc gcgcgccggc cgcctcggcg
     3301 ccggcggcgg tgccgtcggt ggagcgcccg tccaccacga tgacctcgtc caccaggacg
     3361 gacgcgcgtg cggcgcccac cacctcggcg atggtgggcg cctcgttcca cgcggcgatg
     3421 accgctgcgg ccttcacctg acgcgcgccg tcatggcgct gcgcttgttc ggcggcagcg
     3481 tgcccggcag gtgcagcgcg ttttcgacgt cgcgcacgcc ggtgacctgg cgcacctcgt
     3541 gcaccagccg ctcgatctgc tcggggcggt ccagctggcc gttcagcatc accacgccgt
     3601 cggcggcact gacgttgacg gtgtagtggc gccactgctc ggcgcccagc acctcgctgc
     3661 ggaccttgtc caccagcgtc tgggcgttct ccggctgctg gtccgggcgc agctcgcgca
     3721 ccatgccctt ggaccggccc gccaggtact caccgcgctt ggacatgccg cggcggcgcg
     3781 cacgcatcgt gccggcagcc tggctcgcca tgcggtggcg gcgcctggcg cctgcctgcg
     3841 ggtcgaacag gtaggccagc agggcgcccc ccatccctgc cagcagtgtc gggcgaagtc
     3901 tcatcgcgcg ctcctttgcg ttgtcgcctt gaggtctccc tggccgcata cccgcgggcg
     3961 cggcccggca tggctgccgg cgcgcagaat gtcgcgcgac gcgaaaaggc ctcagcgagg
     4021 tggcagcgcg gagatcgcgg cgcccgccac cacgacggcg gcggcgaggc ggatcaccca
     4081 cttggccacg gggcgcagcg gccggcgctc ggccgcgtcg ggctccggcg cgagggcgtg
     4141 cgcggcgtcg gcctcggcca gcagcgcgcg ggcggcgtcg gcgtcctcgg cgggcacgcg
     4201 cagcgagatg ccgcggtaca gcgccacgtg ggggtaggtg ccgccggcgt cgtcggccca
     4261 gacggtggcg gggatgccgt tggcgtgcag gtacgtggcc gcgacctcgg ccatgggccg
     4321 ggagggaaag tgccccacct gttcgaatgc cgccatcgtg ctcactccgt cggcgcgcgt
     4381 cgcctgaccg cccagtatgc gcgccatggc gcgctagggt cggacggaca gatggaacgc
     4441 cacgaaagcg agcgacgacg gcatgagcgg gttcgaggac aagctgcgcg agcggctgga
     4501 gagcaacgcg gcgctggccg agcagcgcgc cgccgccgag gcggagatgg accgcgcggc
     4561 gctgcaggcg caacaggagg ccgccgcggc ggagcgcgaa ttgcgtgcgt cgcgggacgc
     4621 ccgccacgcc gagctggccg cgcgcctgga ggacctgctg gacatgctgc gcgggtccgg
     4681 ccagcggctg gaggtgcgcg ccggctggag cgcgtccggt gaggagttct acgcgcggct
     4741 ggccacgcac gcgggctggc cgcagcgcgt gctgtcgctg gagctggacc gcgacgacga
     4801 cgaggtgctg gcgcggtggc actccgacgt gggcaacacc ctggagctgt ggcgcctgct
     4861 ggagttcgac gtcgagatgc tggaggagct ggtgctccag gtgatcgacg acgagctgtg
     4921 gcgcggcgcc tccgcgccgc ccgccttccc cgagccggct tagccgggcg ggcgcggggc
     4981 ggccagccgc gtcggcgtgg cgcggccctc cacggtgacc tcgccgacca gctcccagtg
     5041 gccgcgctcg gcctcgccgg ccgcgtcgag cgccacggcg gaggccagca ggcgcgacgg
     5101 cgccgacttc gccagctcgg tcagccgggc cgcctcgttc accgggtccc cgatgacggt
     5161 gaactcgaag cggcgctggt cgcccacgtt gccggcgacg acgcggcccg cggccacgcc
     5221 gatgcccgcc gggcactccg gcacctccgc cggaagccgc ccggccagct cgcgcgcggc
     5281 ggcgagcgcc cggccgcagg cgtcgtccag cggggtcggc acgccgaaca ccgccagcgc
     5341 cgcgtcgccg acgaacttgt tcaccagccc gccatggcgg tcgaccacgt cgacgaccac
     5401 ggtgaagaag cggttgagca gctccacgat gcgctcgggc ggcagttccg cagccaggcg
     5461 tgtggagccg acgatgtcca cgaacaccac cgccgcgtcg cgcacctcgc cgcccgcgtc
     5521 gaaccgcccg gccacggccg cggtcgcgac cggctcgccg acgtgccggc cgaacaggtc
     5581 gcgcaggcgc tcgcgctcct ccaggccggc caccatgcgg ttgaagccgg cctgcaggcg
     5641 gccgagctcg gaggcgtcgt agacgacgat gcgcgtgtcc agccgcccgg cttccacggc
     5701 ggcgagcgcg cggcgcatcg agcgcagcgg gtccagcgtg ccctggatgc ccaggcgcac
     5761 cagcagcagg ctcacggcca gcgtcacgcc gcccagtccc agcatcgtga tggcgagcga
     5821 gccggcgggc acgtcgcctt cgaccagcgc caccaccgcc acgaccatca agcccagcac
     5881 cggcacgccc gtgcccacca gccacaccag cagggagcgc accgcgaggc cgggcgcgcc
     5941 gcgctccggt gcgccgggca gggccaggac gcgcgcggcc agtggccgca gtgccagctc
     6001 gctgagcagg taggagcacg tgcacgtcac caggccgccg agcaggacgg tggtcgtggt
     6061 gcgcagcacc acgcgccagc cggccggtac gcccagcgcg ccgaacagca ccacggcgcc
     6121 gagccacagc gcgacctgca ggcccagcag gcggaacggc gcgcgcagca cctggctgtg
     6181 gccgtcgccg ggcggcgcgc cgccctcggc gacccagcgc aggcgcggca gctcccgccg
     6241 ggtgccagac agcgcgccgg cggccacggc gagcacgatg taggcgggcg cggcgacgac
     6301 gaccacggtg ccgaagcgtg tgtcgagcag gctggggccg gggatgagcc agacgctcag
     6361 cgcgacgacg gcggccgcgc ccacgaggtt ggcgacgacg accgcgccgg tgagcagcgt
     6421 ctgcacgcgc agtcgcagcg cccagcccgg ctcgccggcc cggcccagga cgcggaggcc
     6481 gaacggccgc tcacctccgg gaccgctcgt cggccgcgcg tcgtcgtgat gcacccgcgg
     6541 atcgtagatc ccggtctcgg cggccggcag gtggtcaccc cgcacaccac ggggtagaac
     6601 ccgcggcatg aatcgactcg ccgaggcctc cagcccgtac ctgctccagc acgccgacaa
     6661 ccccgtggac tggtgggagt ggggtgagga cgcgttcgcg gaggcgaagc ggcgcgacgt
     6721 gccggtcttc ctgtcggtcg gctactcctc gtgccactgg tgccatgtca tggcgcacga
     6781 gtccttcgag gacgccgagg tggccgcgta cctgaacgag cacttcgtct cggtgaaggt
     6841 ggaccgcgag gagcgccccg acgtcgacgc cgtctacatg ggcgcggtgc aggcgatcag
     6901 cggccgcggc ggctggccca tgagcgtgtt cctcacgccg gacggcacgc cgttcttcgg
     6961 cggcacctac tggccgaagg aggagcgcca gggcatgccg ggcttcctgc gggtgatcga
     7021 ggcggtcagc gcggcgtgga ccaacgagcg cgacaaggtg gtggagtccg gcgggaagct
     7081 ggcccagcac ctgcgcgagc agcagcaggt cgacgccagc gacgagcggc cggacctgac
     7141 catcccggag cgggccgcgg agctgtgcgt gcgcatgtgg gaccgccggc acggcggctt
     7201 cggacaggcg cccaagttcc ctcaggcgat gacgatcgac ttcctgctgg cgcactcgct
     7261 gcgcaccggc gacgccgagg cgaaggaggc ggcgacgcac tcgctgaccg cgatgagcaa
     7321 gggcggcatc tacgaccatg tcgccggcgg cttcgcgcgc tactcggtcg acgacgtctg
     7381 gctggtcccc cacttcgaga agatgctgta cgacaacgcg ctgctgctgc gcgcgtacgt
     7441 gcacggctgg caggccaccg gcaacccccg cttccgccgc atcgccgtgg agaccgccga
     7501 gtacctgctg cgcgacatgc ggcatgcgcg cggggcgttc tactcggcca cggacgccga
     7561 cagcgagggc gaggagggca agttcttcct gtggcacgcc gaggagttct tcgacaccat
     7621 ccaggacggc ggcgagaacc ccgagaagtg gatggcgttc tacggcgtca cgccggcggg
     7681 caacttctcc gacccccacc accccggcag cccgatgcgc agcatcctgt acgaggcgct
     7741 ggagcgcgac gagctggacg acgagttcgc cgcccgcgcg cagaaggtgc gcgagatcct
     7801 ctacgccaag cgcgagcagc gcgtccaccc ggggctggac gacaagatcc tgacgtcgtg
     7861 gaacgcgctg gcgatcggcg cgctggccga ggccggcgcg gcgttcggga tcccgcgctt
     7921 cgtggtggcc gcacagcagg ccgccgcgtt cctgcgtgac gaggcgcagg tccacggccg
     7981 cctgatgcac gtctggaagg agggacgcgg cgcgtcggtg ccggcgttcc tggaggacgt
     8041 ggcgtacctg gcgcaggcgc tgctggtgct gtacgaggcc gacttcgacc cggactggtt
     8101 ccgctgggcg ctgcggctgg cgcagacggc gtgggcggag ttcggcgacg aggagaccgg
     8161 cgcgttctac tcgaccgcgc acgacgccga ggagctgctc acgcggccga aggacctgtg
     8221 ggacaacgcc acgcccgcgg gctcgtccgt catggtggac gtgcacctgc ggctgcacgc
     8281 gctgaccggc gagacccagt ggctggagcg ggcggagaag accatcgcgg cgttccagta
     8341 ccgcgcgctg caggcgccga ccggctacgg ggagttcctg cgcgccatgg agcgcatggc
     8401 gtccgggccg atggaggtgg ccatcgtggg gcagctggag gacgaggcga cgatacggct
     8461 gatcgaggtg taccgggagc gctggcggcc cggcgccgtg gcggcggtca acggcgtggt
     8521 gggcggtgtc gaggccgcgg acggcgccgc ctcgacaccg gacgtgcatc ccgttgcgct
     8581 gctgcacgag cgcccgctgg tcggcgggcg gcccgcggcg tacgtgtgcc ggcagttcgc
     8641 ctgcgagcgc cccgtcacgt cacccgacga cttgcgcgac gccctcgacc gccgcgcctg
     8701 acagtgagaa tgccgtaggg cctgcacccc gcccggtctg actctgcgac tgacagaccg
     8761 tcgtgtcctg aaagggatcc gtcgaccgga tcggggtgca ggttccttgg gcacgagctg
     8821 tcggacgaag tgacctcata ggagcctgcg ctatcaggcg cccatcactg tcttgtccgt
     8881 cgtctggctg tgcgtgccgg cccagcccag ttgacgagga gggacggcga tgaccatggt
     8941 gacagatccg gtggcggcgg cccaggtgct ggcggtgacg ggcggggtgg acacccatgc
     9001 cgaggtgcac gtcgcggcgg cgatcgacgc ggtcggccgc gagctcggcc acgcgtcgtt
     9061 tgcgacgaca ccggcgggtt atgccgcgct gctggcgtgg cttgagagct tcgggccgct
     9121 ggagcgggtc ggggtggagg gcaccggtgt gtacggcgcc gggctcaccc gggtgctgca
     9181 ggccgccggg gtgcgagtga tcgaggtcga ccggcccgac cgcaaggcgc gccgctacca
     9241 gggcaagtcc gatccgatcg acgcttacgc cgccgcccgc gcggccctgt cgggccgtgc
     9301 ggcgggcacg cccaagacgc ggaccggctc ggtggagatg atccgcacgc tacgggtcgc
     9361 gcgggctggt gcggtcaagg ccaaggcggt ggcgtggaac cagctcaagg cgctgatcaa
     9421 gaccgccccg gaggacctgc gtgagcggct gcgcgccctc gacggcgccg cgctgctcga
     9481 cgcttgcgtc gcgctgcgcg ccgcccgcgg ccccgccccc gagcacggcc cgcacgccaa
     9541 gcggcaccgt cccggcgtgc tggccgaccc gaccgccgca tgcaagcgca gcctcgcggt
     9601 gctcgcccgg cgcgtacgcg ccctggaggc cgagatcacc gacctcgaca acgacctcga
     9661 accgctcatc gtcgcgaccg caccgactct gctcggcctg tacggcgtcg gcctcgacgt
     9721 cgccggccaa ctgctggcca cggccggcga caaccctgat cggctggcct ccgaggccgc
     9781 gttcgcgcac ctgtgcggcg tcgcgccgat ccccgcctcc tccggcaaaa ccgaacgcca
     9841 ccggctcaac cgcggcggcg accgccatgc caacaacgcc ctgtggcgca tcgcgatgac
     9901 tcggctgcgc tacgaccccg ccaccaggga ataccgcgac cgccgcgcca aggaacagaa
     9961 gaccaacaaa gacatcttgc gctgcctcaa gcgctacatc gcccgagaag tccaccgcgc
    10021 cctgatggcc gacctcacca ctccatcacc gacttgacat ccataggagc atcc
//
LOCUS       DATMKC010000352         8530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_44977, whole genome shotgun sequence.
ACCESSION   DATMKC010000352 DATMKC010000000
VERSION     DATMKC010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8530
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44977"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VNU01_10275"
     CDS             <1..983
                     /locus_tag="VNU01_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWH33045.1"
                     /translation="VEPAARERFSLAGQVIRGHDRLRVVSVERLGVTIPMYDITTGTG
                     DFIANGVVSRNCFARPTHTYLNLDAFGDFEKTVVVKTNVADVLRHELSKKSWTGEHVA
                     MGTNTDPYQRCEGRYRLMPDIIQALADGLTPFSILTKGTLITRDLDVLTAAARRVEVR
                     AALTIGMLDEALWRSSEPGTPSPRARLDAVRALTEAGIPTGVMLAPIMPGLNDDPGQL
                     AELVDAAADAGATHVTPIVLHLRPGVREVFWPWLQRTHPELEGRYAALYRRSTADKSY
                     REPVEQYVRARRRIAFRLRGRQEPREYLRASQPAPPGQTAGHAGDQLTLL"
     gene            complement(1026..1481)
                     /locus_tag="VNU01_10280"
     CDS             complement(1026..1481)
                     /locus_tag="VNU01_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWH33046.1"
                     /translation="MGRRYRLGVLRRLGNLFMSVAVLLGLPRSTVLLETVGARTGRVR
                     RTPVNVLRLDDERWLVAPYGLVGWVHNVRAAPAATLRRGLVGEPVRLEPADDDVAGPV
                     LKAYWEAFPITRPFFDAGEDAPPEAFAGEAARHPVFRVVAADPAGKRAI"
     gene            1636..3111
                     /locus_tag="VNU01_10285"
     CDS             1636..3111
                     /locus_tag="VNU01_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33047.1"
                     /translation="MGRDIDTVTISPEHRARFRGRLLENQEALRRMIADGRFGFVRRR
                     VGVELEASLVGPDGDPIGVNAELLELLAASGLDFQSELGRFNIEFASPPLPLGGGALD
                     ALERSLADTLDGARGCADTLGARPVLIGILPTITDFDVAEQNISVNPRYRMLNDAMLA
                     ERGEQPHLHIEGADTLDLEIPSIAYAAACTSLQIHLEVRPEEFSAHWNAATAASAALV
                     AASANAPLFLGRRLHHESRIAVFSQACDTRSRELAAQGVAPRVWFGSGWVSDGALELF
                     EQNLRWFPALLPLPEGDSPLDILDRGEIPPLAALTLHNGTIYRWNRPVYGVMEGGPNL
                     RIENRVMPAGPTIADVAANVALCLGLMRALALEERPLWERLPFGVAASNFERAARDGI
                     HARLAWPGVQGDLPACELLLRLLPLAREGLAELGVATAEADRCLGIVEGRAHRRRNGA
                     VWQLETWQALRDGGRDPRDAARELVRRYLECAATGEPVHTW"
     gene            3105..4136
                     /locus_tag="VNU01_10290"
     CDS             3105..4136
                     /locus_tag="VNU01_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HWH33048.1"
                     /translation="MVSGDPLYLRVEGHDAFAPYLASDEETLVRLGKPALLRIPGRDP
                     ARARFVSCLLHGNEDSGYRAVLRLLRRRPVFDFDVWVFIGNVRAAHQHGWFAHRYLDD
                     QEDFNRVWGVAERTTRMRQCAAAVLQELSGAPLEAALDIHNNTGANPPYAIVPVPTQQ
                     AQALAATCSVMVVHWNRRAHTLMEALSALCPAVSVECGLPGIEEHHRYAEGALARFLA
                     VEDPTTGPVPERVFELLATVHVRREVAFAFGGPLTDELDLVLAAGLDGHNFGTMPAGA
                     VLGHVRPGADMPLVALDMDRTDVTARHFEVCPDGSVVATRALTPAMMVTTVQQERRDC
                     LFYVARRLV"
     gene            complement(4112..5563)
                     /locus_tag="VNU01_10295"
     CDS             complement(4112..5563)
                     /locus_tag="VNU01_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWH33049.1"
                     /translation="MPAASRPRYDAVVVGAGPNGLSAAVTLAMAGRGVLVVEGADRIG
                     GGARTAEVTLPGFAHDLCSAIHPLAVATPFLPRLPLDRHGLEWVHPEVLVAHPLDDGR
                     AGVLRRSLEDTADGLGDADGRAWRRLFRAPLHAWDDVAGQLVAPLSLPRHPLALAGTA
                     LQALLPARGVMRGMFTGEEAQALFTGAAAHAFMRLDAPFSAAFGLVLIAAGHAHGWPL
                     ARGGSQAIADALASYLVELGGEIVTGWQVRSVEELPPAEQYLLDVTPRQLLGVAGHRF
                     PPRYQRQLQRYAYGPGAFKVDYALSGPVPWRNADARRAGTVHVAGTMEELAAAEHAVV
                     QGRVPERPFVLVAQQSLFDGTRAPAGTHTLWAYCHVPHGCTEDMTERMERQIDRFAPG
                     WRDLVLARATRGPADLEAYNPNFVGGDIAGGNHGVHKLLAGPTLRLRPHTTPDPTIFL
                     CSSSTPPGPGVHGLCGWHAARAALLAQTSRRAT"
     gene            complement(5593..6462)
                     /gene="mca"
                     /locus_tag="VNU01_10300"
     CDS             complement(5593..6462)
                     /gene="mca"
                     /locus_tag="VNU01_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HWH33050.1"
                     /translation="MNRFHAMFVHAHPDDESSKGAATMARYAKEGHRVSVVTCTDGGA
                     GDILNPAMDRPGVKERIVEIRREELAEALEILGVADHWWLGYPDSGYVEGFEGDGTLL
                     PADCFYNAPMDEAVERLVRIVRAERPDVLVTYPEGGGYPHPDHIRTHDITVAAFDAAG
                     DASRYPDAGPPWTTKKLYYVGAFNRRKVEALHAACAERGIESPFTGWLERWDPDEKDP
                     STTQVDVGEFLGHRTRALIAHATQVDPAGMWFKVPDEVIREVYPFEDYTLARSVVPVQ
                     LPEDSLFAGLPDE"
     gene            complement(6497..7354)
                     /locus_tag="VNU01_10305"
     CDS             complement(6497..7354)
                     /locus_tag="VNU01_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWH33051.1"
                     /translation="MVERLRVSVVVIGDEILGGFVQDTNSHWLAVRLHELGVPLERVS
                     TVPDQLDAIREALLTELGRARPRLVLTCGGIGSTPDDLTMEAVARSLGLGLERQPDID
                     DRITVALEWTARQGVDVTAEHERSMRKMAMVPEGAYLLSGAQGVAPGVALDVDGGCKA
                     AGGATIVILPGVPAELKRIMEHGIQPLLLEGRGEPLHVAELRHPYPESTLNPVLERIV
                     AEYPDVHLGSYPGQECIIRLKGAPERVEAAMALVRAHVEALAADAGAQRLREAWQERW
                     SGDSPRPAS"
     gene            7436..8101
                     /locus_tag="VNU01_10310"
     CDS             7436..8101
                     /locus_tag="VNU01_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33052.1"
                     /translation="MYSSPDDARSDLFLAGAVFLFGPLVVQILLDIAPLPDFEPLFVL
                     VDVAVPIAVTVLAPYLLIRYRKEPLGAYGFSGVQTQNLALGALLGLPLAIASVLALLV
                     RFGPETANPLALTTVGGAPMIATRLAHAIGRCLLAVYVTVKARDAFRQDYLAVREGLR
                     DIGRWVVIGSAGALLLLTLSAVLRGQGIGLLVGVLTETKRGAWGAIGLGGVIALVTIF
                     QTV"
     gene            8251..>8530
                     /locus_tag="VNU01_10315"
     CDS             8251..>8530
                     /locus_tag="VNU01_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33053.1"
                     /translation="MTDVLEGAPKRARSFPYARAQALITVAAVILFADLGMVASTIPA
                     MTPPLTQSSAEAAADALLHTPSEARPSVERAARADTSPVVPAPAPAAPA"
BASE COUNT         1090 a         3215 c         3079 g         1146 t
ORIGIN      
        1 cggtggaacc cgcggcgaga gagcgcttct ccctcgccgg gcaggtgatc aggggtcatg
       61 accggctgcg cgtggtgtcg gtggaacggc tcggtgtcac gatcccgatg tacgacatca
      121 ccacgggcac gggagacttc atcgcgaacg gcgtcgtcag ccgcaactgc tttgcgcggc
      181 cgacgcacac gtacctgaac ctggacgcgt tcggcgactt cgagaagacc gtcgtggtga
      241 agaccaacgt cgccgacgtc ctgcgccacg agctgtccaa gaagagctgg acgggcgagc
      301 acgtcgcgat ggggacgaac acggacccct accagcgctg cgagggccgc taccggctga
      361 tgccggacat catccaggcg ctcgcggacg gcctcacgcc gttctccatc ctcaccaagg
      421 gcacgctgat cacccgcgac ctggacgtgc tcacggcggc cgcgcggcgc gtcgaggtcc
      481 gcgccgcgct gaccatcggc atgctcgacg aggcgctgtg gcgctcctcc gagccgggca
      541 cgccctcgcc ccgcgctcgc ctggacgccg tgcgggcgct caccgaggcg ggcatcccca
      601 caggcgtcat gctggcgccg atcatgccgg gcctgaacga cgaccccggg cagctcgccg
      661 aactggtcga cgccgcggcg gacgccggcg ccacgcatgt cacgcccatc gtgctgcacc
      721 tgcgccccgg tgtgcgcgag gtgttctggc cctggctcca gcgcacgcac ccggagctgg
      781 agggcaggta cgcggcgctg taccggcgct ccaccgcgga caagtcctac cgcgagccgg
      841 tcgagcagta cgtgcgggcg cggcgccgca tcgcgttccg cctgcgcggg cggcaggagc
      901 ccagggagta ccttcgcgcc agccagcccg caccgccggg gcagacggcc ggccacgcgg
      961 gcgaccagtt gacgctgctg tgaccgaagg gcgagacgtg ggcaacccgc gctgcgacac
     1021 gggcctcaga tggcgcgctt cccggcagga tccgcagcga cgacgcggaa caccgggtgc
     1081 cttgcggctt cccccgcgaa cgcttccggt ggtgcatcct ctccggcatc gaagaacggc
     1141 cgggtgatcg ggaacgcctc ccaataggcc ttcagcaccg ggcccgcgac gtcgtcgtcc
     1201 gcgggctcca gtcgcacggg ctcgcccacc aggccgcggc gcaaggtcgc cgcgggcgcg
     1261 gcgcggacgt tgtgcaccca gcccaccagc ccgtacggcg cgaccagcca gcgctcgtcg
     1321 tccagcctga gcacgttcac cggcgtgcgc cgcacccggc cggtgcgcgc ccccaccgtc
     1381 tccagcagca cggtcgagcg cggcagcccc agcagcaccg ccacgctcat gaacaggttc
     1441 cccaggcgcc ggagcacccc cagccgatag cgccgcccca tgccggtcac tcctttcacc
     1501 ggatcccggc gggctcagcc taaccctggg ggcacctggg cgatgggacg actccgcgcc
     1561 cggtacgcgc acgggtcatc ggggcaagct cctgctccgg cggccgcatt ggcggccgtc
     1621 gggcacggag gagcaatggg ccgcgacatc gacacggtca ccatcagccc ggagcaccgg
     1681 gcgcgcttcc gcggccgcct gctggagaac caggaggcgc tgcggcgcat gatcgccgac
     1741 ggccgcttcg gcttcgtccg gcgccgcgtc ggcgtggagc tggaggcgtc gctggtcggc
     1801 ccggacggcg accccatcgg cgtgaacgcc gagctgctgg agttgctggc ggcgtcgggc
     1861 ctggacttcc agagcgagct cggccgcttc aacatcgagt tcgccagccc gccgcttccg
     1921 ctgggcggcg gcgcgctcga cgcgctcgag cgctcgctgg ccgacaccct ggacggcgcc
     1981 cgcggctgcg ccgacacgtt gggcgcccgg cccgtgctga tcggcatcct gccgaccatc
     2041 accgacttcg acgtggccga gcagaacatc tcggtgaacc cccgctaccg catgctcaac
     2101 gacgcgatgc tggccgagcg cggggagcag ccgcacctgc acatcgaggg cgcggacaca
     2161 ctggacctgg agatcccgag catcgcctac gccgccgcgt gcacgtcctt gcagatccac
     2221 ctcgaggtgc ggcccgagga gttcagcgcc cactggaacg ccgccacggc ggcctcggcc
     2281 gcgctggtgg ccgcgtccgc gaacgcgccg ctgttcctcg gccggcggct gcaccacgag
     2341 tcgcgcatcg ccgtgttctc ccaggcgtgc gacacgcgct cgcgcgagct cgccgcgcag
     2401 ggcgtcgcgc cgcgcgtgtg gttcggctcg ggctgggtca gcgacggcgc gctggagctg
     2461 ttcgagcaga acctgcgctg gttccccgcg ctgctgccgc tgccggaagg ggactcgccg
     2521 ctggacatcc tggaccgcgg cgagatcccg ccgctggccg cgctgaccct gcacaacggc
     2581 accatctacc gctggaaccg gccggtgtac ggcgtcatgg agggcggtcc gaacctgcgc
     2641 atcgagaacc gcgtcatgcc ggccgggccg accatcgccg acgtcgccgc gaacgtggcg
     2701 ctctgccttg gcctcatgcg tgccctggcc cttgaggagc gtccgctgtg ggagcggctg
     2761 ccgttcggcg tggccgcgtc caacttcgag cgcgcggcgc gcgacggcat ccacgcacgg
     2821 ctcgcgtggc caggcgtcca gggcgatctg ccggcctgcg agctgctgct gcgcctgttg
     2881 ccgctggcgc gcgagggcct cgcggaactg ggtgtggcca cggcggaggc ggaccggtgc
     2941 ctggggatcg tggaggggcg cgcgcaccgg cgccgcaacg gcgcggtgtg gcagctggag
     3001 acctggcagg cgctgcgcga cggcggcagg gatcctcgcg acgcggcgcg cgaactggtg
     3061 cgccgctacc tggagtgcgc ggcgaccggg gagccggtgc acacatggtg agcggcgacc
     3121 cgctgtacct gcgcgtggag ggccacgacg ccttcgcccc gtacctggcc tccgacgagg
     3181 agacgctggt gcgcctgggc aagccggcgc tcttgcgcat cccgggacgt gaccccgcgc
     3241 gcgcccgctt cgtgagctgc ctgctgcacg gcaacgagga ctcgggctac cgggcggtcc
     3301 tgcgcctgct gcggcggcgg ccggtgttcg acttcgacgt gtgggtgttc atcggcaacg
     3361 tgcgcgccgc gcaccagcac ggctggttcg cccaccgcta cctggacgac caggaggact
     3421 tcaaccgggt gtggggcgtg gccgagcgca ccacccgcat gcgccagtgc gccgccgcgg
     3481 tgctccagga gctgtccggc gccccgctgg aggccgcgct ggacatccac aacaacacgg
     3541 gcgcgaaccc cccgtacgcg atcgtgcccg tgcccaccca gcaggcccag gcgctggcgg
     3601 cgacctgctc ggtcatggtg gtgcactgga accgccgtgc ccacacgctg atggaggcgc
     3661 tgtcggcgct ctgcccggcg gtgtcggtgg agtgcggcct gcccgggatc gaggagcacc
     3721 accgctacgc ggagggcgcg ctcgcgcgct tcctggcggt ggaggatccg accaccggcc
     3781 ccgtgccgga gcgcgtcttc gagctgctgg cgaccgtgca cgtgcgccgc gaggtggcgt
     3841 tcgccttcgg cgggccgctc accgacgagc tggacctggt ccttgccgcc ggcctggacg
     3901 gccacaactt cggcacgatg ccggccggcg ccgtcctggg ccacgtccgt cccggggccg
     3961 acatgccgct ggtcgcgctg gacatggacc gcaccgacgt cacggcgcgc catttcgagg
     4021 tgtgcccgga cggctccgtg gtggccacgc gcgcgctcac cccggcgatg atggtcacca
     4081 ccgtgcagca ggagcgccgc gactgcctgt tctacgtcgc gcgccggctg gtctgagcca
     4141 gcagcgcggc gcgcgcggcg tgccagccgc acagcccgtg gacgccgggc ccgggcggcg
     4201 tcgacgagga gcacaggaag atcgtggggt ccggcgtggt gtgcgggcgc agccgcagcg
     4261 tcgggccggc caggagcttg tgcaccccgt ggttgccgcc ggcgatgtcg ccgcccacga
     4321 agttggggtt gtacgcctcc aggtccgccg ggccccgcgt cgcccgcgcc aggacgaggt
     4381 cgcgccagcc gggggcgaag cggtcgatct ggcgctccat gcgttcggtc atgtcctccg
     4441 tgcagccgtg cggcacgtgg cagtacgccc acagcgtgtg tgtgcccgcg ggcgcgcgcg
     4501 tgccgtcgaa caggctctgc tgggcgacca gcacgaacgg ccgctccggc acgcggccct
     4561 gcaccaccgc gtgctcggcc gccgccagct cctccatcgt cccggcgacg tgcaccgtcc
     4621 cggctcgccg cgcgtcggcg ttgcgccacg gcaccggccc ggacagcgcg tagtcgacct
     4681 tgaacgcgcc cggcccgtag gcgtagcgct gcagttggcg ctggtagcgc ggcgggaagc
     4741 ggtgcccggc cacgcccagg agctgccgcg gcgtgacgtc gagcaggtac tgctcggcgg
     4801 gcggcagctc ctccacggaa cgcacctgcc agccggtgac gatctcgccg ccgagctcca
     4861 ccaggtatga ggccagcgcg tcggcgatcg cctgcgagcc gccgcgcgcg agcggccagc
     4921 cgtgggcgtg gcccgccgcg atcaggacca ggccgaacgc ggcgctgaac ggcgcgtcca
     4981 ggcgcatgaa cgcgtgcgcc gccgcgccgg tgaacagcgc ctgcgcctcc tcgcccgtga
     5041 acatgccgcg catcacgccg cgggcgggca gcagcgcctg cagcgccgtg cccgccagcg
     5101 ccagcggatg gcgcggcagc gacagcggcg ccaccagctg tccggcgacg tcgtcccagg
     5161 cgtgcagcgg cgcgcggaac agccggcgcc aggcgcgccc gtccgcgtcg ccgaggccgt
     5221 ccgcggtgtc ctccagggag cggcgcagca ccccggcgcg gccgtcgtcc agcgggtggg
     5281 ccaccagcac ctcggggtgc acccactcca ggccgtggcg gtccaggggc agccgcggca
     5341 ggaacggcgt cgccacggcc agcgggtgga tcgccgagca caggtcgtgg gcgaagccgg
     5401 gaagcgtcac ctcggcggtg cgcgcgccgc cgccgatgcg gtccgcgccc tccaccacca
     5461 gcacgccgcg gccggccatg gccagggtca cggccgccga cagcccgttc gggccggcgc
     5521 ccaccaccac cgcgtcgtac ctcgggcggg acgcggctgg catcggggcg ctcctggctt
     5581 cggcggtggg cgctactcgt cgggcaggcc cgcgaacagc gagtcctccg gcagctgcac
     5641 cggcacgacg ctgcgggcca gggtgtagtc ctcgaacggg tagacctcgc ggatgacctc
     5701 gtccggcacc ttgaaccaca tacccgccgg gtccacttgt gtcgcatgcg cgatcagcgc
     5761 cctggtgcgg tgccccagga actcgcccac gtcgacctgc gtggtcgagg ggtccttctc
     5821 gtcggggtcc cagcgctcca gccagccggt gaacggcgac tcgatgccgc gctcggcgca
     5881 cgcggcgtgc agcgcctcca ccttgcggcg gttgaacgcg ccgacgtagt acagcttctt
     5941 cgtcgtccac ggcggcccgg cgtccgggta gcgcgaggcg tccccggccg cgtcgaacgc
     6001 ggcgaccgtg atgtcgtgcg tgcggatgtg gtccgggtgc gggtagccgc cgccctccgg
     6061 gtaggtgacc agcacgtcgg gtcgctcggc gcgcacgatg cggaccaggc gctcgaccgc
     6121 ctcgtccatc ggcgcgttgt agaagcagtc cgccggcagc agcgtgccgt cgccctcgaa
     6181 gccctcgacg tagccggagt ccgggtagcc cagccaccag tggtcggcga cacccaggat
     6241 ctccagcgcc tccgcgagct cttcccggcg gatctcgacg atgcgctctt tgaccccggg
     6301 ccggtccatg gccgggttga ggatgtcgcc ggcgccgccg tcggtgcagg tcaccacgga
     6361 cacgcggtgg ccctccttgg cgtagcgcgc catggtggcg gcacccttgg acgactcgtc
     6421 gtccgggtgg gcgtgcacga acatcgcgtg gaaccggttc atgcccgcca gcctagcgat
     6481 ggtccgcgac ggcgactcag ctcgcggggc ggggagagtc cccggaccag cgctcctgcc
     6541 acgcctcgcg caggcgctgc gcgcccgcgt cggcggccag cgcctcgacg tgcgcgcgca
     6601 ccagcgccat cgccgcctcg acgcgttcgg gcgcgccctt cagccggatg atgcactcct
     6661 ggccgggata ggagcccagg tgcacgtccg ggtactcggc gacgatgcgc tccaggaccg
     6721 ggttcagcgt cgactccggg tagggatgac gcagttccgc gacgtgcagc ggctcgccgc
     6781 ggccctccag cagcagcggc tggatgccat gctccatgat ccgcttcagc tcggcgggca
     6841 ccccgggcag gatcacgatg gtggccccgc cggcggcctt gcacccgccg tcgacgtcca
     6901 gcgcgacgcc cggcgcgaca ccttgcgccc cggacagcag gtaggcgcct tccgggacca
     6961 tggccatctt gcgcatcgag cgctcgtgct cggcggtcac gtcgacgccc tggcgggccg
     7021 tccactccag cgccacggtg atgcggtcgt cgatgtcggg ctggcgctcc aggcccaggc
     7081 ccaggctgcg cgccaccgcc tccatggtga ggtcgtcagg cgtggagccg atccccccgc
     7141 aggtcagcac caggcgcggg cgggcccgcc ccagttccgt gagcagcgcc tcgcggatcg
     7201 cgtcgagctg gtccgggacg gtgctgaccc gctccagcgg cacccccagc tcgtgcaggc
     7261 gcacggcgag ccagtgggag ttggtgtcct ggacgaagcc ccccaggatc tcgtcgccga
     7321 tcacgacgac cgacacgcgc agccgctcca ccatgcgcgc gagcatagcg gggctccggc
     7381 gggtcgcgtc cggcccgggg gcggtaccgc tgcgccgccc cgtaggatgc gacccatgta
     7441 ctccagtccc gacgacgcgc gctcggacct gttcctggcc ggcgcggtct tcctgttcgg
     7501 tcccctggtc gtccagatcc tgctggacat cgcgccgctg ccggacttcg agccgctgtt
     7561 cgtgctcgtc gacgtggcgg tcccgatcgc ggtcaccgtc ctggcgccgt acctgctgat
     7621 ccgctaccgc aaggagccgc tcggcgccta cgggttctcc ggggtgcaga cgcagaatct
     7681 cgccctgggc gcgctgctcg gtctgccgct ggcgatcgca tccgtcctgg cgctgctcgt
     7741 ccgcttcggc ccggagacgg cgaatccgct ggcgctgacg accgtcggcg gcgcgccgat
     7801 gatcgcgacg cggctcgcgc acgcgatcgg ccgctgcctg ctggccgtgt acgtgaccgt
     7861 caaggcgcgc gacgcgttcc gccaggacta cctggccgtg cgcgagggcc tgcgcgacat
     7921 cggccgctgg gtggtgatcg gctcggcggg cgcgctgctg ctgctcacgc tgagcgccgt
     7981 gctgcgcggg cagggcatcg gcctgctcgt gggcgtgctc acggagacca agcgcggcgc
     8041 ctggggggcc atcggcctgg gtggcgtgat cgccctggtc accatcttcc agaccgtcta
     8101 gggccgcacg cccggcgcgc gatcttgacg gcaatctcgg gccgatcacc ggacgcgtca
     8161 cggtccccgg gctaccctcg ttggccagat cggagccctt cacgtggggc tccggtcgtg
     8221 cacgcacgac agacgagagg ctcaccgatc gtgaccgacg ttcttgaggg cgcgccgaag
     8281 cgcgcgcgtt cctttccgta cgcccgcgcg caggcgctca tcaccgtggc cgcggtgatc
     8341 ctgttcgccg acctcggcat ggtcgcctcg acgatccccg ccatgacgcc gcccctgacg
     8401 cagtccagcg ccgaggccgc cgcggacgcg ctgctgcaca ccccctcgga agcccgtccc
     8461 agcgtggaac gtgccgcgcg cgccgacacg agcccggtgg tgcccgcgcc cgctccggca
     8521 gccccggccc
//
LOCUS       DATMKC010000353         9720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_175405, whole genome shotgun sequence.
ACCESSION   DATMKC010000353 DATMKC010000000
VERSION     DATMKC010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175405"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VNU01_10320"
     CDS             complement(<1..640)
                     /locus_tag="VNU01_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HWH33054.1"
                     /translation="MNRTAEGHGNSVDEAIQQALRQLGLTADQVFVEILSEGSRRGLF
                     GGRRAHVRAIAKDAVVAPGTTSETTSSEPQQTEEDGVTANDQEDVTAEAVATRDEDAM
                     EADGDGQARESLREQMTPEEWLDLLDQEAEMAADFVEGLLDRLDLPGDLEIEVTEDQA
                     LVNVQDLGSGLLIGRRGATLDALQELVRSAVQRQTQRRAHVRVDVEGYRARQL"
     gene            complement(688..1434)
                     /locus_tag="VNU01_10325"
     CDS             complement(688..1434)
                     /locus_tag="VNU01_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HWH33055.1"
                     /translation="MGELLTSVAEALGEVLRVLHSLVEPVTGAAAWGWAIILLTLLVR
                     VVMLPLAVKQINSMRAMQRLQPEIKKLQAKYKTDRSMMRTDPEKYRERRAKQQEEMMK
                     LYKEHNVNPAAGCLPLLLQMPIFFALFRLLSSTEIVPQLNTAGFYFAQLAERPTQAGL
                     GAWALIALMGYTTYFSQKQMMASNPGAAENPQQRMMLYLMPVVLTVFAVNLQTGVLLY
                     WVTTNIWTMAQQWVMFRGLDHERAAKGDKA"
     gene            complement(1457..1699)
                     /gene="yidD"
                     /locus_tag="VNU01_10330"
     CDS             complement(1457..1699)
                     /gene="yidD"
                     /locus_tag="VNU01_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWH33056.1"
                     /translation="MSRSPLAVVLLLLVRLYRAVPKRGGTCRFHPSCSAYAAEALETH
                     GGMRGAWLAMRRLGRCHPFHPGGLDPVPPRRRAVLN"
     gene            complement(1696..2106)
                     /gene="rnpA"
                     /locus_tag="VNU01_10335"
     CDS             complement(1696..2106)
                     /gene="rnpA"
                     /locus_tag="VNU01_10335"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWH33057.1"
                     /translation="MITSARAAGGSARTVRRSNTTQSNSPGLPSGVPELLRDGQAAHG
                     AAVVVRALPRRGDEPRRAVVASRKVGGAVARNRAKRRLREAMRAAGISPDVDVVVIAR
                     GAALSVPMPQLTAELRALAGRLEEKLAVAAKGAQ"
     gene            complement(2141..2275)
                     /gene="rpmH"
                     /locus_tag="VNU01_10340"
     CDS             complement(2141..2275)
                     /gene="rpmH"
                     /locus_tag="VNU01_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWH33058.1"
                     /translation="MKRTYQPNNRRRKRKHGFRSRMQDRAGQAIVKARRSKGRKRLTA
                     "
     gene            3055..4485
                     /gene="dnaA"
                     /locus_tag="VNU01_10345"
     CDS             3055..4485
                     /gene="dnaA"
                     /locus_tag="VNU01_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWH33059.1"
                     /translation="MPDVSVVDLEDLWNRSLTDLQTHLTGAAQRAWLDDTRPVGFSQD
                     TVVLAAPHSFAREQLDTKYGAVLRGALTRAAGRPLEVVVTVRPDGEAASSRAEALAEA
                     DAAAEEAVRVPHPRHEAAPERFTAPGDRASELNEKYTFDRFVIGASNRFAHAASFAVA
                     EAPAKAYNPLFIYGGAGLGKTHLLQAIGHYTMSLYPGMQVRYVTSEQFTNEFIDAISN
                     GRQVPFQRRYRDVDVLLIDDIQFLESKERTQEEFFHTFNALHNAEKQIVISSDRPPKQ
                     IGQLEERLRTRFEWGLITDIQPPDLETRLAILRKKAVQDNLPVPDDVLELIASRISSN
                     IREIEGALIRVTAFASLQRTEVTLQLAQVVLKDLFPGTESSEIGVSLIMSETANYFGL
                     TIEDLCSTSRTRQLVTARQIAMYLTRELTELSLPKIGAAFGGRDHTTVIHANNKIAGL
                     MQERPAIYQQLQELTSRIKSAARQSR"
     gene            4841..5959
                     /gene="dnaN"
                     /locus_tag="VNU01_10350"
     CDS             4841..5959
                     /gene="dnaN"
                     /locus_tag="VNU01_10350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWH33060.1"
                     /translation="MKFRAERVEFADAASWALHTVGARATRPALSGVRLEVRGDRLEF
                     SSTDLDMSSVLSLGVHAERDGVALVPGRLLAELVRSLPNAAVEAEANGDELFLSCGRA
                     QFHLRLMPMDDFPVLPEPAAGASAAVMKADEFARMVVQVARAASLDGARPALTGVQLE
                     ATAGSLTAAATDSYRLAVCTVPWDQGADTTVLVPRKALEAARRSAEQLGSEVRLVLES
                     EQVTFEFGDRRLTTQLIGHKFPDFRQLIPSGYERRLTVDRAELADVVKRIAVIGDSNT
                     TATPVTLHLSADTVRITAGSGEVGRAEEALPGSLEGEELTIAFNPRYLSDGLEAAGSE
                     RVVLEFRDELKPAVLRPATSEGEERTADFLYLLMPVRV"
     gene            5980..7134
                     /gene="recF"
                     /locus_tag="VNU01_10355"
     CDS             5980..7134
                     /gene="recF"
                     /locus_tag="VNU01_10355"
                     /inference="COORDINATES: protein motif:HMM:NF000822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HWH33061.1"
                     /translation="MRLETLELADWRSYERARLDLGPGVRVIVGDNAQGKTNLLEAVH
                     YLAIGSSHRVSSDAPLVRHGADAAIVRARALLGATDAAGAQALSVELEVRPQGRNRVR
                     VNGQPQNRPRDAMGLIRSVLFAPEDLSLVRGDPSDRRRFLDELLGQRRPAYLAARQEY
                     ERVLKQRNALLKHVRGGFADRADLEVWTDGLVAAGAGLLAARIAAVHALAGPVAEAYA
                     DLVSASPEREATGAVEMVYELSTGRRVAAEPGAGMPEPAELAAELAEGLARLASAERE
                     RGITLAGPHRDELYLGLKGLPAKGYASHGEHWSLALALRLASREVLLEVGEEPIVLLD
                     DVFAELDERRRARLAERCAGFEQVLVTAAVDHDVPLAGPRSMVRAGTVRS"
     gene            7131..7502
                     /locus_tag="VNU01_10360"
     CDS             7131..7502
                     /locus_tag="VNU01_10360"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HWH33062.1"
                     /translation="MSWSGLRGQQGEDPDARPEGKAPAPLGQLVGAIAGRRGWRTRLE
                     GARIHERWGDIAGEQLAAHTAPVRLHGGVLVLRVASAAWATQVRYLTGELMTRANEVL
                     GERQVTSVQIVIGAVDGAGDR"
     gene            7733..>9720
                     /gene="gyrB"
                     /locus_tag="VNU01_10365"
     CDS             7733..>9720
                     /gene="gyrB"
                     /locus_tag="VNU01_10365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWH33063.1"
                     /translation="MTTTNDYGAADITVLEGLEAVRKRPGMYIGSTGPRGLHHLVWEV
                     VDNSVDEAMAGRCDRIEITIQADGGVRVSDNGRGIPVDEHPTMRKSALEVVLTVLHAG
                     GKFDGKSYAVSGGLHGVGVSVVNALSSRLVADVKRDGKLYRQSFTVGVPDGPVQVIGE
                     APDGETGTTITFWASEDVFETTDYSWETLITRLRETAFLTAGLEIVLVDERHSAITQD
                     GGETGEPRKAVFKYDDGLQDFVRHLNTAKDTVHDEIVHFSASETDADGPHEVEVALQW
                     NTGYHESIHTFANTINTHEGGTHEEGFKKALTNVVNRFARDLGLLKPTGKEKDLTLTG
                     EDIREGLTAIISVKLADPQFEGQTKTKLGNTPIRSFVERTCNDQLRRWFEEHPNEVRV
                     IVNKAIQGARARMAARQARELTRRKGFLEGAALPGKLADCQSKDPEESEIFVVEGDSA
                     GGSSKMARDRVTQAVLPIRGKILNVEKARLGKILENKEIQALITAIGTGIGEEFDLSK
                     ARYHKIVMLMDADVDGAHIRTLVLTFLFRHMRELIEAGYVYIAQPPLYQIKPAGSKDK
                     DKIRYAYSDRQRDQILAELRNGESNRKIEIGRFKGLGEMDAEQLWDTTMDPAVRTLLQ
                     VGMEDAAAADQMFTVLMGDDVEARRDFIVRNARCRASSP"
BASE COUNT         1498 a         3455 c         3397 g         1370 t
ORIGIN      
        1 cgagctggcg agcccgatac ccctcgacgt cgacccgcac gtgggcgcgc cgctgcgtct
       61 ggcgctgcac ggctgagcgt acgagttcct gcagcgcgtc gagcgtcgcg ccacggcggc
      121 cgatcagcag gccgctgccg agatcctgga cgttgacgag cgcctggtcc tcggtgacct
      181 cgatctccag gtcgccgggc aggtccagcc ggtcgagcag tccttccacg aaatcggcgg
      241 ccatctcggc ctcttggtcc agcagatcca gccattcctc tggggtcatc tgctcgcgca
      301 ggctttcccg tgcctgcccg tcgccatcgg cctccatggc gtcctcgtcg cgtgtggcca
      361 cagcctcggc tgtgacatct tcctggtcgt tcgcggtcac cccgtcctcc tcggtctgct
      421 ggggttcgct gcttgtggtc tcactcgtgg tccctggggc gacgacggcg tccttggcaa
      481 tcgcccgcac atgcgcgcgg cgcccgccga acaggccccg gcggctgccc tcggagagaa
      541 tttcgacgaa cacctgatcc gccgtgagac ccagctggcg cagggcctgc tggatcgcct
      601 cgtcgacgga gtttccatgc ccctccgcgg tgcgattcac tgcctccctc cccttctggt
      661 ggccgatcgt cgccggccgc ccgtgtccta ggccttgtcg cccttggccg cccgctcgtg
      721 gtcgagtccg cggaacatca cccactgctg cgccatggtc cagatgttgg tggtgaccca
      781 gtacagcagc acgcccgtct gcaggttcac ggcgaacacc gtcagcacca cgggcatcag
      841 gtacagcatc atccgctgtt gcggattctc ggcggcgccg gggttgctcg ccatcatctg
      901 cttctgggag aagtacgtcg tgtagcccat gagcgcgatc agcgcccacg cccccaggcc
      961 cgcctgcgtc gggcgctcgg ccagctgggc gaagtagaac cccgccgtgt tgagctgggg
     1021 gacgatctcg gtgctgctca gcaggcggaa cagcgcgaag aagatcggca tttgcaacag
     1081 gagcggcagg caacccgcag cggggttgac gttgtgctcc ttgtacagct tcatcatctc
     1141 ttcttgctgc ttggcccgcc gctcccggta tttctccggg tccgtccgca tcatcgagcg
     1201 gtcggtcttg tacttggcct gcagcttctt gatctccggt tgcaggcgct gcatggcccg
     1261 catggagttg atctgcttca ccgccagggg aagcatcacg acgcgcacga gcagcgtcag
     1321 caggatgatc gcccagcccc aggcggccgc accggtgacc ggctcgacga gcgagtgcaa
     1381 gacgcgcagc acctcgccga gcgcctcggc cacggaagtc aacagctcgc ccacgtgtgt
     1441 gtccctctcc ctctcgtcag ttcagcacgg cgcggcgccg tggaggcacg gggtccagcc
     1501 cgcccgggtg gaacggatga cagcggccga ggcggcgcat ggccagccag gccccgcgca
     1561 tgccgccgtg ggtctccagc gcctcggcgg cgtacgccga gcagctcggg tggaagcggc
     1621 aggtgccgcc gcgcttcggc acggcccgat agagccgcac gagcagcagc agcacgacgg
     1681 ccaggggact gcggctcact gtgccccctt ggcggccacg gccagcttct cttcgaggcg
     1741 gccggcgagc gcccgcaatt cggcggtcag ttgcggcatg gggacgctga gggcggcccc
     1801 gcgagcgatg accacgacgt ccacgtcggg cgagatcccc gcggcacgca tcgcctcccg
     1861 cagccggcgc ttcgcgcgat tgcgcgcgac ggcaccaccg actttgcggc tggccaccac
     1921 ggcacgccgg ggctcgtcgc cgcgacgggg gagcgcgcgc acgaccacgg ccgcgccgtg
     1981 cgccgcctgg ccgtcccgca gcagctcggg gaccccggat ggcaggccgg gtgagttact
     2041 ctgcgtagta ttagaacgac gcacggtgcg cgccgatccc cccgcggccc gggccgaggt
     2101 gatcatcgtc cctgcgcccc gctcacggaa aatggccgag tcaggcggtc agtcgcttgc
     2161 ggcccttgct gcggcgcgcc ttgacgatcg cctgaccggc acggtcctgc atgcgcgagc
     2221 ggaaaccgtg cttgcgcttg cgccggcggt tgttcggctg gtacgtgcgc ttcatggact
     2281 gctcactcca gacaagcttg tcgaccgctg ggccgctgag gtgtggcctg gcgacccggg
     2341 tccttccggg tgggacgaga acccaacggt accgctgacc gctcggcgct tcctgtggtt
     2401 cactcgcgca caaaggcgcc tggggggcac gctcgcgggc atggcgacac ctgcggacgc
     2461 gaacgcccga agcacgcagt gtaggaacgc cgcgggaagc ctgtcaaacg gaccgggcag
     2521 ccgggccgcc gcagcccccg cccgcgcgtg attccggccc gtaccaccgc tgccggatga
     2581 tcacggcaag cccctgacct gcggattcgc ggctttgacg cgtccctgtg gaaaagtgct
     2641 tgcggcgccg gaaaggcgct ccctatactc gccaacgttc ccacggggac ggttgctcgg
     2701 cgtggcggaa aggccaaggc ggcaacttcg ggaccatgac ggcaaagcgc tcaacttcgt
     2761 cgggatggcc tcgcccgtgg gcgcaccgcg agacgcacac cgcttccctg gtgctcggcc
     2821 gccggctccg cgccggcgac gcaccccggt ggaaccggcc tgacgccgac cgcgcgctcc
     2881 gcaggtctct gcggggggtg ctgttgacgt acccctcatc cacagatgtg gaggatgttg
     2941 tggataaggc tggcctgacg ccgcccgcgg gttttcgccg tgagaccggt cacggtgaaa
     3001 cagccgaatg aaccagccgt cccgctcccg cgaggcggac aggaaggacc aagggtgccg
     3061 gacgtgtcgg ttgtcgacct cgaagacctc tggaaccgct cgctcaccga cttgcagacg
     3121 cacctgacgg gtgccgccca gcgtgcctgg ctggatgaca ccaggcccgt gggcttcagt
     3181 caggacaccg tcgtgctggc cgcgccgcac agcttcgcca gggagcagct ggacaccaag
     3241 tacggcgccg tgctgcgcgg cgccctgacg cgcgccgcgg gccgcccgct ggaggtggtc
     3301 gtcaccgtcc ggccggacgg cgaggccgcg agcagccggg ccgaggcgct cgcggaagcc
     3361 gacgcggccg cggaggaggc cgtccgcgtg ccgcatccgc gccacgaggc cgcgcccgag
     3421 cgcttcaccg cgccgggcga ccgcgccagc gagctcaacg agaagtacac cttcgaccgc
     3481 ttcgtcatcg gcgccagcaa ccgcttcgcc cacgccgcgt cgttcgcggt ggccgaggcg
     3541 ccggcgaagg cgtacaaccc gctgttcatc tacggcggcg cgggactggg caagacgcac
     3601 ctgctgcagg cgatcgggca ctacaccatg agcctgtatc ccggcatgca ggtgcgctac
     3661 gtgacctcgg agcagttcac caacgagttc atcgacgcca tctccaacgg ccggcaggtg
     3721 ccgttccagc gccggtaccg ggacgtggac gtgctgctca tcgacgacat ccagttcctg
     3781 gagtccaagg agcgcacgca ggaggagttc ttccacacgt tcaacgcgct gcacaacgcc
     3841 gagaagcaga tcgtcatctc ctcggatcgc ccgcccaagc agatcggcca gctcgaggag
     3901 cggctgcgca cgcggttcga gtgggggctg atcaccgaca tccagccgcc ggacctggag
     3961 acccgcctgg cgatcctgcg caagaaggcc gtgcaggaca acctgccggt gccggacgac
     4021 gtgctggagc tgatcgccag ccgcatctcg tcgaacatcc gcgagatcga gggcgcgctc
     4081 atccgcgtga ccgcgttcgc cagcctccag cgcaccgagg tgaccctcca gctggcccag
     4141 gtggtgctca aggacctgtt cccgggcacg gagagctccg agatcggggt gagcctgatc
     4201 atgtcggaga ccgccaacta cttcgggctg accatcgagg acctgtgctc gaccagccgc
     4261 acgcggcagc tggtgaccgc ccggcagatc gcgatgtatc tcacccgcga gctcaccgag
     4321 ctgtccttgc ccaagatcgg cgcggcgttc ggcggacgcg accacaccac ggtcatccac
     4381 gccaacaaca agatcgccgg cctcatgcaa gagcggccgg ccatttacca gcagctccag
     4441 gagctcacca gccgcatcaa gtcggcggcg aggcagtcgc gttgagcgcc tgcacacggg
     4501 ctcaggaacc cgatggcgcg gctgtgggcg agctgtgggc agcgtcgtgg acggcctggg
     4561 ggaaaaccgc gattctcccc agctcgtgtt tcacatcgct gtcatctggg gagaacgggc
     4621 cgcttcccca cacggcgtgc acaggcggcc cacagtcacc ccacaggccc cgtaagctcc
     4681 tgaccggcgg aaagaggggt tgtccccagc actgccgccc ctctacgagt acgacggatt
     4741 ttctctatcc aacaagcttg aagaaccggg tcccggcctt tgtggggaca accggcgatt
     4801 cgcatggcat gtccggcaca tcggcaccac ggaggacggc atgaagttca gggcggagcg
     4861 agtggagttc gccgacgcgg cgtcatgggc cctgcacacc gtgggtgccc gtgccacgcg
     4921 gccggcgctg tccggcgtgc ggctcgaggt gcgcggcgat cgcctggagt tctcctccac
     4981 cgatctggac atgtcgtcgg tgctctcgct cggggtccac gccgagcgcg atggtgtcgc
     5041 cctcgtgccg ggccgcctgc tggccgagct ggtgcgcagc ctgcccaacg ccgccgtgga
     5101 ggcggaagcc aacggcgacg agctgttcct gtcctgcggc cgggcgcagt tccacttgcg
     5161 gctcatgccg atggacgact tccccgtgct gccggagccg gcggcgggcg cgtccgcggc
     5221 cgtcatgaag gccgacgagt tcgcccgcat ggtcgtgcag gtggcgcgcg ccgccagcct
     5281 ggacggcgca cggccggcac tcaccggcgt gcagctcgag gccaccgccg gctcgctcac
     5341 cgcggccgcc acggactcct accggctggc cgtgtgcacc gtgccgtggg accagggcgc
     5401 ggacaccacc gtgctggtgc cccgcaaggc gctggaggcc gcgcggcgct cggccgagca
     5461 gctcggcagc gaggtgcggc tggtcctgga gagcgagcag gtgacgtttg agttcggcga
     5521 ccggcgcctg accacgcagc tgatcggcca caaattcccc gacttccgcc agctcatccc
     5581 gtccggctac gagcggaggc tgaccgtcga ccgtgccgag ctcgcggacg tggtcaagcg
     5641 catcgcggtg atcggtgaca gcaacaccac cgccacgccc gtgacgttgc acctgtccgc
     5701 cgacacggtg cgcatcaccg cgggcagcgg cgaggtgggc cgggcggagg aggcgctgcc
     5761 gggcagcctg gaaggcgagg agctcaccat cgcgttcaac ccgcgctacc tgtccgacgg
     5821 cctggaggcg gcgggcagcg agcgcgtcgt gctggaattc cgcgacgagc tcaagcccgc
     5881 ggtgctgcga ccggccacca gcgagggcga ggagcgcacc gccgacttcc tctacctgct
     5941 gatgccggtc cgggtctgac cgccgcggcg cccgccgcga tgcgcctgga gacgctggag
     6001 ctggccgact ggcgctccta cgaacgcgcc cgcctggacc tgggacccgg cgtgcgcgtc
     6061 atcgtgggcg acaacgccca gggcaagacc aacctcctgg aggcggtgca ctacctggcc
     6121 atcggctcca gccaccgggt gtcttcggac gcgccgctgg tgcgccatgg cgccgatgcc
     6181 gccatcgtcc gggcgcgggc gctgctgggc gccaccgacg cggcgggtgc gcaggcgctg
     6241 tcggtggagc tggaggtccg cccgcagggc cgcaaccggg tccgggtgaa cggccagccc
     6301 cagaaccgcc cgcgcgacgc gatggggctc atccgcagcg tgctgttcgc gccggaggac
     6361 ctgtcactgg tccgcggcga cccgtccgac cgccgccgct tccttgacga gctgctgggg
     6421 cagcgccggc ccgcctacct ggccgcgcgg caggagtacg agcgcgtgct caagcagcgc
     6481 aacgcgctgc tcaagcacgt tcgcggcggc ttcgccgatc gggcggatct ggaggtctgg
     6541 accgatgggc tggtggcagc cggcgcgggg ctgctggcgg cccggatcgc ggcggtgcac
     6601 gcgctggccg gtcccgtcgc cgaggcgtac gcggacctgg tgagcgcatc gccggagcgg
     6661 gaggccaccg gggcggtgga gatggtctac gagctgtcca ccgggcggcg cgtcgcggcg
     6721 gagccgggcg cgggcatgcc cgagcccgcc gagctcgccg cggagctggc ggagggtctg
     6781 gcccgcctgg cctcggcgga gcgcgagcgg ggcatcacgc tggccgggcc gcaccgcgac
     6841 gagctgtacc tgggcttgaa gggcctgccg gccaagggct acgccagcca cggcgagcac
     6901 tggtcgctcg cgctggcgct gcgcctggcg agccgggagg tcctgctcga agtgggggag
     6961 gaacccatcg tgttgctgga cgacgtcttc gccgaactgg acgagcgccg ccgcgcccgc
     7021 ctcgccgagc gctgtgccgg cttcgagcag gtgctggtca ccgcggcggt ggaccacgac
     7081 gtgccgctgg ccggcccccg ctccatggtc cgcgccggaa cggtgcggtc atgagctggt
     7141 cgggactgcg cggccaacag ggcgaggatc cggacgccag gcccgagggc aaggcgccgg
     7201 cgccgctggg ccagctggtg ggcgccatcg ccggccggcg cgggtggcgc acgcggctgg
     7261 agggagcacg gatccacgag cgctggggcg acatcgccgg tgagcagctg gccgcgcaca
     7321 cggcaccggt ccggctgcac ggcggcgtgc tggtgctgcg cgtggcgtcc gcggcatggg
     7381 ccacacaggt gcgctacctc acgggcgagc tgatgacccg ggccaacgag gtgctcggcg
     7441 agcggcaggt caccagcgtg cagatcgtga tcggggcggt ggacggcgcg ggcgacaggt
     7501 gaccacccgg acacgttgtc cacagggaag gccgctgacc tgcggaaacg gctccagagg
     7561 cacggacggt cgccgaccgt ggcgtggtag gcttacaggc atcgaagcgg tccgggacgc
     7621 ctgagcgccc gacgcgcaag attttgccga tcttcaccga aatcgggctc cggcttgcgc
     7681 ttcccccgcg cgccgagccc ctgctgcccg gacaccgggc ccggagcgac gtgtgaccac
     7741 gacgaacgac tatggcgccg ccgacatcac ggtcctggaa ggcctggagg cggtccgcaa
     7801 gcggccgggc atgtacatcg gctcgaccgg cccgcgcggg ctgcaccacc tggtctggga
     7861 ggtggtcgac aactccgtcg acgaggcgat ggcgggccgc tgcgaccgca tcgagatcac
     7921 gatccaggcc gacggcggcg tgcgcgtctc cgacaacggc cgcggcatcc cggtggacga
     7981 gcaccccacc atgcgcaagt ccgcgctgga ggtcgtgctg accgtcctgc acgccggcgg
     8041 caagttcgac ggcaagagct acgcggtctc cggcggcctg cacggcgtgg gcgtgtccgt
     8101 ggtgaacgcg ctgtcctccc ggctggtcgc cgacgtcaag cgcgacggca agctgtaccg
     8161 ccagtccttc accgtcggcg tgccggacgg gccggtgcag gtcatcggcg aggcgccgga
     8221 cggcgagacc ggcaccacga tcacgttctg ggccagcgag gacgtgttcg agaccacgga
     8281 ctactcgtgg gagacgctga tcacccgcct gcgcgagacc gcgttcctga cggcgggcct
     8341 ggagatcgtg ctcgtcgacg agcgccacag cgccatcacg caggacggcg gcgagaccgg
     8401 cgagccgcgc aaggcggtgt tcaagtacga cgacggcctg caggacttcg tccgccacct
     8461 caacacggcc aaggacaccg tccacgacga gatcgtgcac ttctccgcca gcgagaccga
     8521 cgccgacggc ccgcacgagg tcgaggtcgc cctgcagtgg aacaccggct accacgagtc
     8581 gatccacacg ttcgcgaaca cgatcaacac ccacgagggc ggcactcacg aggagggctt
     8641 caagaaggcc ctgaccaacg tggtcaaccg cttcgcccgc gacctcgggc tcctgaagcc
     8701 caccggcaag gagaaggacc tcaccctcac gggcgaggac atccgcgagg gcctgaccgc
     8761 gatcatctcg gtgaagctgg ccgacccgca gttcgagggc cagaccaaga cgaagctggg
     8821 caacacgccc atccgcagct tcgtggagcg cacctgcaac gaccagctcc gccgctggtt
     8881 cgaggagcac cccaacgagg tccgcgtcat cgtcaacaag gccatccagg gcgcccgcgc
     8941 ccgcatggcc gcccggcagg cgcgcgagct cacccgccgg aagggcttcc tggagggtgc
     9001 ggcgctgccc ggcaagctgg ccgactgcca gtccaaggac cccgaggagt cggagatctt
     9061 cgtggtggag ggcgactccg ccggcggcag ctccaagatg gcgcgcgacc gcgtgaccca
     9121 ggcggtgctg cccatccgcg gcaagatcct caacgtcgag aaggcgcgcc tgggcaagat
     9181 cctggagaac aaggagatcc aggcgctcat caccgcgatc ggcacgggca tcggcgagga
     9241 gttcgacctc agcaaggccc gctaccacaa gatcgtcatg ctcatggacg ccgacgtcga
     9301 cggtgcccac atccgcaccc tggtgctgac gttcctgttc cgccacatgc gggagctgat
     9361 cgaggcgggc tacgtctaca tcgcccagcc gccgctgtac cagatcaagc ccgcgggctc
     9421 caaggacaag gacaagatcc gctacgccta ctccgaccgc cagcgcgacc agatcctcgc
     9481 cgagctgcgc aacggcgaga gcaaccgcaa gatcgagatc ggccgcttca agggcctcgg
     9541 cgagatggac gccgaacagc tgtgggacac gaccatggac ccggcggtgc gcacgctgct
     9601 ccaggtcggc atggaggacg ccgcggccgc cgaccagatg ttcaccgtgc tcatgggcga
     9661 cgacgtggag gcccgccgcg acttcatcgt gcgcaacgcc cgctgccgtg cgtcatcgcc
//
LOCUS       DATMKC010000354         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_318450, whole genome shotgun sequence.
ACCESSION   DATMKC010000354 DATMKC010000000
VERSION     DATMKC010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318450"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..973)
                     /locus_tag="VNU01_10370"
     CDS             complement(95..973)
                     /locus_tag="VNU01_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWH33064.1"
                     /translation="MTALSRAPGYADEARRPRMAFFTDTTVCIGCKACEVACKEWNDV
                     PDSLWDFTGHSYDNTGHLSADAWRHVAFVEQPTHLQGAEQMAHTAAIDAHAEALATDG
                     FRWLMMSDVCKHCTTAGCLDNCPTGALFKTEFDSVVVQPDICNGCGYCVVGCPYGVIS
                     RRESDGKAWKCTMCYDRLRVGEQPACAKTCPTDSINFGSLEAMRELARTRLEQVHALG
                     YGGAHLYGEADSDAAGVDGTGALFLLLDEPEVYGLPPDPVAPTKTLGSSWMAALGAMG
                     ALAVALLGAVAGGGRR"
     gene            complement(970..>2111)
                     /locus_tag="VNU01_10375"
     CDS             complement(970..>2111)
                     /locus_tag="VNU01_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWH33065.1"
                     /translation="PTEGEVADPDPEAVLAEINGRHADGTPVASLTELRADGSTACGC
                     WIYAGCMAGGVNHTARRTPWHEQGPVALEWGWAWPSNRRMLYNRASADPEGRPWSERK
                     RYVWWDQEQGRWSGEDVPDFQATKPPSYRPAADAVGPDAIAGDDPFIMQADGKGWLFV
                     PAGLTDGPMPTHYEPHESPFANALYGQRANPARGRYDVAVNPYQTADGTGGGDAYPFV
                     VTTYRLTEHHTAGGMSRTLGHLAELQPEMFCEVSPTLAAARGLTHGGWATIVTSRTAI
                     EARVLVTERMKPLRVQGRTVEQVGLPYHWGSNGLVTGDSANDLFPIVVDPNVKIQEVK
                     AATCDVLPGRRPRGPLLRQLVERWPAEHVALRRDGWRPERGEVGV"
BASE COUNT          252 a          805 c          755 g          299 t
ORIGIN      
        1 gtgcccgtct acttcttcgt gggcgggacc atcgcgccct tggggctcgt gagctgctgc
       61 gcgggcaccc cgggcgcgcc ctggcgcgtg gcgctcatcg gcggcccccg ccggccacgg
      121 cgccgagcag cgcgacggcg agcgccccca tggcgcccag cgccgccatc cacgacgagc
      181 cgagcgtctt ggtgggcgcc accgggtccg gcggcagccc gtacacctcc ggctcgtcca
      241 ggagcaggaa cagcgcgccg gtgccgtcca cgcccgccgc gtcggagtcg gcctcgccgt
      301 acaggtgcgc gcccccgtac ccgagcgcgt gcacctgctc caggcgcgtg cgcgccagct
      361 cgcgcatcgc ctccagcgac ccgaagttga tcgagtcggt ggggcaggtc ttggcgcacg
      421 ccggctgctc gcccacccgc aggcggtcgt agcacatcgt gcacttccac gccttgccgt
      481 cggactcgcg ccgcgagatc acgccgtacg ggcagcccac cacgcagtag ccgcagccgt
      541 tgcagatgtc cggctgcacc accacggagt cgaactcggt cttgaacagc gcgccggtgg
      601 ggcagttgtc cagacacccc gccgtggtgc agtgcttgca gacatccgac atcatcagcc
      661 agcggaaacc gtccgtggcc agcgcctccg cgtgcgcgtc gatcgcggcg gtgtgcgcca
      721 tctgctcggc gccttggaga tgcgtgggct gttcgacgaa agcgacgtgg cgccaggcgt
      781 ccgcggacag gtggccggtg ttgtcgtagg agtgcccggt gaagtcccac agggagtccg
      841 ggacgtcgtt ccactccttg caggcgacct cgcaggcctt gcagccgatg cacacggtgg
      901 tgtcggtgaa gaacgccatg cgcggccggc gcgcctcgtc ggcgtacccc ggcgcccgcg
      961 acagcgcggt cacacgccca cctccccgcg ctcgggccgc cagccgtcgc ggcgcagcgc
     1021 cacgtgctcg gcgggccagc gctcgaccag ctggcgcagc agcgggccgc gcgggcgccg
     1081 ccccggcagc acgtcgcagg tcgcggcctt gacctcctgg atcttcacgt tcgggtcgac
     1141 cacgatcggg aacaggtcgt tggccgagtc gccggtgacc aggccgttgc tgccccagtg
     1201 gtacggcagg ccgacctgct ccaccgtgcg gccctgcacg cgcagcggct tcatgcgctc
     1261 ggtcaccagg acgcgtgcct cgatcgcggt gcgcgaggtg acgatggtgg cccagccccc
     1321 gtgggtcagg ccccgcgccg ccgccagggt cggcgacacc tcgcagaaca tctccggctg
     1381 cagctccgcc aggtgcccca gcgtgcggct catcccgccg gcggtgtggt gctcggtgag
     1441 ccggtaggtg gtcaccacga acgggtaggc gtccccgccg cccgtgccgt cggcggtctg
     1501 gtacgggttg accgcgacgt cgtagcggcc gcgcgccggg ttggcgcgct ggccgtacag
     1561 cgcgttggcg aacggcgact cgtgcggctc gtagtgcgtc ggcatggggc cgtcggtcag
     1621 cccggccggc acgaacagcc agcccttgcc gtcggcctgc atgatgaacg ggtcgtcccc
     1681 ggcgatcgcg tccggcccca cggcgtcggc ggccgggcgg tacgacggcg gcttcgtcgc
     1741 ctggaagtcg ggcacgtcct cgccgctcca gcggccctgc tcctggtccc accacacgta
     1801 gcgcttgcgc tccgaccagg gccggccctc cgggtcggcg gacgcgcggt tgtacagcat
     1861 ccgccgattc gacggccacg cccagcccca ctccagcgcg accggcccct gctcgtgcca
     1921 cggcgtgcgc cgcgcggtgt ggttcacgcc gccggccatg cagccggcgt agatccagca
     1981 gccgcacgcg gtggagccgt cggcgcgcag ctccgtgagg ctcgcgaccg gcgtgccgtc
     2041 ggcgtggcgg ccgttgatct cggccagcac cgcctccggg tccgggtcgg ccacctcccc
     2101 ttccgtgggg t
//
LOCUS       DATMKC010000355         3979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_154433, whole genome shotgun sequence.
ACCESSION   DATMKC010000355 DATMKC010000000
VERSION     DATMKC010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154433"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VNU01_10380"
     CDS             complement(<1..279)
                     /locus_tag="VNU01_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33066.1"
                     /translation="MVLQETELIDSALRLLQSRLPPGWTVAQAEGQGAGSGEGDGLFA
                     FNAQSGMGMGTAIVQAKCNFGPADVDRLLGGLTRRLREAAGPRPILLVS"
     gene            complement(884..958)
                     /locus_tag="VNU01_10385"
     tRNA            complement(884..958)
                     /locus_tag="VNU01_10385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Gly,seq:gcc)
     gene            complement(1156..2115)
                     /gene="dut"
                     /locus_tag="VNU01_10390"
     CDS             complement(1156..2115)
                     /gene="dut"
                     /locus_tag="VNU01_10390"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWH33067.1"
                     /translation="MLRVQRNPGLDEALVTELVGIWTRVTNAGGAVGFVAPVTEGDVR
                     PAADEAFAAVRAGRDDLVVAYAGDRAVGWGLLMPNQWATHAHWGWIRRVQRDPEHRAA
                     GVGDAVLSALEEAALDRGLERVALSVRGGTGREGFYLDRGFRVEGYLPGRVRLGPGDD
                     RDEVVMSKKLTGTPDGPLLRVRRLDPEVPLPSYAKAGDAGLDLCAARDVELAPGERAV
                     IPTGIAVALPPGTVGLVHPRSGLAARHGLALVNAPGTIDEGYRGEVKVIAINLDPSET
                     LRLARGERIAQLVVQRVERVRVEESEELDDTERGDGGLGSTGR"
     gene            2225..2785
                     /locus_tag="VNU01_10395"
     CDS             2225..2785
                     /locus_tag="VNU01_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HWH33068.1"
                     /translation="MRPLTRREWEEHYTDLRAPLLKEWPEVDERRLEAANDDFDRVIE
                     LIQAATGMSGDEIESRLKALDVEELGLGTGGPGEEQGAEGAASLDLLRLGGDFDEADR
                     QRVVERLDKLNRRLKKFPADGTDIHLTCKDRDSTGQKVTLELWLPKFGHFAATSTEAD
                     MRAALMDVREDMWRQIDDAVNKRKGN"
     gene            2785..3186
                     /locus_tag="VNU01_10400"
     CDS             2785..3186
                     /locus_tag="VNU01_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33069.1"
                     /translation="MGATLRYAKVIDADRFTAQGSQLRPGLDSEVRLSGEPPAVARPF
                     LIVRSWEREDGFTETWRIADPHGRTLREGLARTVMDSDDDIADEVRDLEFEYADTGYQ
                     LILEVDGREVARVDFPVLEPSPAEQGHMTET"
     gene            complement(3183..3614)
                     /locus_tag="VNU01_10405"
     CDS             complement(3183..3614)
                     /locus_tag="VNU01_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWH33070.1"
                     /translation="MAKPQRHPLARLGFALSGTIVRGRGSAVDAGVAHLRRSGMIWLA
                     PEGGSFRGHLRRPRSGAAVLAAQAGVPIVPLGIRYATEAGPRLRDWRPWRRPEVTVVI
                     GEALALAPGEAPEAAAARYMPALAACAGTVYAPAAEDLATA"
     gene            3607..>3979
                     /locus_tag="VNU01_10410"
     CDS             3607..>3979
                     /locus_tag="VNU01_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33071.1"
                     /translation="MAIGTHARVQHHRQRPVQGVGVVADHHSRPPRQRTAHVHPQGGA
                     EHGPRHGGEQQPGDADALDQAGVQHQPRPGGQRDAGVHGARAAGLVQQRRAEQRHQAG
                     QLQHGERGGELLRAPRQLLDAR"
BASE COUNT          602 a         1513 c         1373 g          491 t
ORIGIN      
        1 gctcacgaga aggatggggc ggggcccggc cgcctcccgg agccgccggg tcaatcctcc
       61 gagcagccgg tcgacatcgg cagggccaaa gttgcacttg gcctgaacga tcgcggtgcc
      121 catccccatg cccgactggg cgttgaaggc gaagaggccg tctccctcgc cgctgcctgc
      181 cccttggcct tccgcctgcg cgaccgtcca ccctggcggg aggcgcgatt gcaatagcct
      241 tagggcagag tcaatgagct cggtttcctg gagaaccatg ccactacttt acaggcaact
      301 ctagccggtt gtctactgcc taaagtttct cgatatggga aaacagctgg ttctcggcaa
      361 ctgtgcgccc gccggcaccg cacccgtcag gcgttcggcg ctcggttgct atcgaacaca
      421 ctccccggcc caagtacacg ctgacgctgt cgaggcgttg ggcgaagcgt tcacgcatct
      481 gggactacaa acggctacta agagcccgaa cgcggacgga tcatcgccgg catcgtcacg
      541 actcatgacg acgaggccga cttcgctttg tgggagctca acacggagcg ccaccggcgg
      601 cgtccgcctt tcttcttgga gctcagatac gccgggtcca ggtgcacgcc tgcgaggacc
      661 ctaggcgcta ggaacggaca ctaagcggtc cgagcgaccg agacccaaag agtctgtccg
      721 caggagccag tcgcattttc ccacagattt cccacaacgt ggtgccggtg agggccggtg
      781 gaggccggaa ttggccggtg ggacgaggag ccggccagtc aggtcgccag cgcaaaaccg
      841 ctggtagaac gcggaaagcc cgccgactgg cgggctttca cgttggagcg gacgacggga
      901 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagcc acgtccgcgt
      961 gcttccggca cggcgcgtgc agcgcgcccg ccatgcgggg acccatcctg ccgcgcgggg
     1021 actgcacctg caagccgagc gccggaatcg cccgctccgc cgtctcgttc accgccaatg
     1081 tgaaccgttg cgctgaaata tcagcgcaac ggttcactgt ggacgcaagg cgacggcgga
     1141 ggcgacggcg gggggtcagc ggccggtgga gcccaggccg ccgtcgccgc gctcggtgtc
     1201 gtccagctcc tcggactcct ccacgcgcac ccgctccacg cgttgcacca ccagctgcgc
     1261 gatgcgctcg ccgcgcgcca gtcgcagcgt ctcggacgga tcgaggttga tcgcgatgac
     1321 cttgacctcg ccgcggtagc cctcgtcgat ggtccccggc gcgttcacca gtgccagccc
     1381 gtgccgcgcc gccagccccg acctcgggtg caccagcccc acggtccccg gcggcagcgc
     1441 caccgcgatc cccgtgggga tcaccgcccg ctcccccggc gccagctcca cgtcccgcgc
     1501 cgcgcacagg tccagcccgg cgtcgccggc cttggcatag gacggcaggg gcacctcggg
     1561 gtccagccgg cgcacgcgca gcagcggccc gtccggcgtc ccggtcagct tcttggacat
     1621 gaccacctcg tcacggtcgt cgccgggccc caggcgcacg cgcccgggca gatagccctc
     1681 cacgcggaag ccccggtcaa ggtagaagcc ctcgcggccg gtgcccccgc gcacggacag
     1741 cgccacgcgc tccagcccgc ggtccagcgc ggcctcctcc agcgcggaca gcaccgcgtc
     1801 gccgaccccg gcggcgcggt gctccgggtc gcgctgcacc cggcggatcc agccccagtg
     1861 cgcgtgcgtc gcccactggt tcggcatcag cagcccccag ccgacggcgc ggtcgccggc
     1921 gtaggccacc accaggtcgt cgcggcccgc ccgcactgcg gcgaacgcct cgtccgcggc
     1981 cggccgcacg tcgccctcgg tgaccggcgc cacgaagccc acggcgccgc cggcgttggt
     2041 cacgcgcgtc cagatcccca ccagctcggt gaccagggcc tcgtcgaggc cggggttgcg
     2101 ctggacgcgg agcacgtgtc ctcctgacgg gcctggcggg tgtggggtcg gtcgcggacg
     2161 ggcagcactc taaccagcgc cgggccccat cccgcgatcc gatccgaaca ggagacagcg
     2221 acgcatgaga ccgctgaccc gccgcgagtg ggaagagcac tacacggacc tgcgggcccc
     2281 cctcctcaag gagtggcccg aggtcgacga gcggcggctg gaggccgcca acgacgactt
     2341 cgaccgcgtg atcgagctca tccaggccgc caccggcatg tccggcgacg agatcgagtc
     2401 gcgcctgaag gccctggatg tcgaggagct gggcctgggc acgggcggcc ccggcgagga
     2461 gcagggcgcc gagggcgccg cctccctgga cctgctgcgc ctgggcggcg acttcgacga
     2521 ggccgaccgg cagcgcgtgg tggagcggct ggacaagctc aaccggcgcc tgaagaagtt
     2581 cccggccgac ggcaccgaca tccacctcac ctgcaaggac cgcgactcca ccgggcagaa
     2641 ggtgacgctg gagctgtggc tgccgaagtt cggccacttc gccgccacct cgacggaggc
     2701 cgacatgcgc gccgccctga tggacgtgcg cgaggacatg tggcggcaga tcgacgacgc
     2761 cgtgaacaag aggaagggca actgatgggc gccaccctcc gctacgccaa ggtcatcgac
     2821 gccgaccgct tcaccgcgca gggcagccag ctccgccccg gccttgactc cgaggtgcga
     2881 ctgtccggcg agccgcccgc cgtggcgcgg ccgttcctga tcgtgcgctc ctgggagcgc
     2941 gaggacggct tcacggagac ctggcgcatc gccgacccgc acgggcgcac gctgcgcgag
     3001 gggctcgcgc gcaccgtgat ggacagcgac gacgacatcg ccgacgaggt ccgcgacctg
     3061 gagttcgagt acgccgacac cggctaccag ctcatcctgg aggttgacgg ccgcgaggtc
     3121 gcccgcgtgg acttccccgt cctggagccc tcccccgccg agcagggcca catgaccgag
     3181 acctaggcgg tggccaggtc ctccgcggcc ggcgcgtaca ccgtgccggc gcaggcggcc
     3241 agcgccggca tgtagcgggc ggcggccgcc tccggcgcct cccccggcgc caatgccagc
     3301 gcctcgccga tcaccacggt gacctcgggg cgccgccacg gccgccagtc gcgcagccgc
     3361 ggtccggcct cggtggcgta gcggatcccc aggggcacga tcggcacgcc ggcctgcgcg
     3421 gccagcaccg ccgcgccgct gcgaggcctg cgcaagtgcc cgcggaagct gccgccctcc
     3481 ggcgccagcc agatcatccc gctgcggcgc aggtgcgcca ccccggcgtc cacggcgctg
     3541 ccgcggccgc gcacgatggt ccccgacagc gcgaagccca ggcgcgccag cggatggcgc
     3601 tgcggcttgg cgatcgggac gcacgcgcgc gtccagcatc accgccagcg gcccgtccag
     3661 ggcgttgggg tggttgctga ccaccacagc cggcccccgc ggcagcgcac cgcgcacgtc
     3721 cacccgcagg gcggcgcgga gcacgggccg cgccacggtg gtgaacagca gcccggcgac
     3781 gccgatgcgc tcgaccaggc gggcgtgcag caccagcccc gcccaggcgg ccagcgcgat
     3841 gccggcgtgc acggcgcccg tgccgccggc ctggtgcagc agcggcgcgc cgagcagcgc
     3901 caccaggctg gccagcttca gcacggcgaa cgcggcggcg aactcctccg ggcgccgcgc
     3961 cagctgctgg atgcgcgac
//
LOCUS       DATMKC010000356         6876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_64014, whole genome shotgun sequence.
ACCESSION   DATMKC010000356 DATMKC010000000
VERSION     DATMKC010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64014"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1574
                     /locus_tag="VNU01_10415"
     CDS             192..1574
                     /locus_tag="VNU01_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWH33072.1"
                     /translation="MSGPPEPASTPDPDSTRSRRRGADARPRRDWRLPVPDNVLLRRF
                     GYVRLVGFGAWLLACIVLYGIFGTQMWPIALGLPVLAVVTGGYFARSTRYPRTAVVVS
                     LLADALVLGGAIAFLGGSGSGLVMLYAIVVVSAGILLGPGAATVFGLLTVFLGVLQLV
                     IEEMGFPPVLLHRPELDDRLPILLASLAGLVSVGYLSTTYAGRLHELLALADEEAEQV
                     RERGRRRRSFVRQASIDVREPLLEVERVADELDERWADMTESERRRMAARLRMRVTEL
                     DAEIGQLEDLGAMDETGEQRLVPVLLPRAVADVVVALGERLDGYVIDIQVPQIKVLGS
                     RRGARRVIYNLMENVIQHTPLGTGLRVTGLTTAGHGVLVFTDDGPGIPSNVAHRLFDA
                     PDEGGGPRVGLPLVAELCAAMGADIRYEPAPSGGSRFMVAFRLAPSGAPSADDEAPAD
                     AAVSGPAAAD"
     gene            1619..2518
                     /gene="dapA"
                     /locus_tag="VNU01_10420"
     CDS             1619..2518
                     /gene="dapA"
                     /locus_tag="VNU01_10420"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWH33073.1"
                     /translation="MRDVPIFGRVLTAMVSPFTADGALDLDGAQRLAAHLVDHGSDGI
                     VVAGSTGESPTLTHDETLDLVRAVKEAVGDRAKVVVGTGKNDTAATVAMSREVAGIGV
                     DGLLLVSPYYNRPSQRGMLAHFGAVADAVGGLPVLLYDIPSRTGCEIAKETMATLAER
                     HANVVGVKDAAGDFPKSAWLVSRTPEHFELYAGDDVATLPLLAVGGVGVISVAAHLVG
                     DDLAAMVDAFPTDPGKAREIHFKLLPLFSTLFCDVNPVPLKAALRLVGLPGGPLRLPL
                     AEPDDQTVQRVREALAAVGVLSA"
     gene            2515..4197
                     /locus_tag="VNU01_10425"
     CDS             2515..4197
                     /locus_tag="VNU01_10425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWH33074.1"
                     /translation="MSDPMQRGRRASASPVEVSFYGGLGEIGANMASVEVDGKIALID
                     VGLIFPDAEHHGIDLILPDWSRLRERAEDVHCVVITHGHEDHMGALPFFLRDFPGVPI
                     YSSRLALGLIGAKLEEHPDVTFTPVEVEAGERIETGPFDIEFLSVTHSIPDGMGVAFH
                     TPHGTILHTGDFKLDQTPIDGRPTDLPHFAQLGDEGVALLLADSTNADRPGHVKTERI
                     VGDTLRDVFAAATGRVVVTTFASHVHRVQQAIDAAVEVGRKVCFVGRSMVRNMPIARD
                     LGYLNYRDEHVVEMEDVEKMARDATVIVCTGSQGEPYAALSLMAAGQHRQVRLEPGDT
                     VVMASSVIPGNEHAIFKSINGLFRQGADVVHTGIADVHVSGHAAADELRILHNIVRPN
                     AVIPVHGEYRHLVAHRKIAIETGVAEDNVLVCEDGDTILLEDGEVRRGESFQAGRVFV
                     DGLGVGDVGNAVLRDRSRLSSEGVCVAVLVTDQHDRLDGEPMVVQQGIIYEPEAGDLL
                     DQAAKVLGEELRRVKDGEEAAVLRRITVQTLARYWKEQTGRRPVILPIVVEV"
     gene            4206..6740
                     /locus_tag="VNU01_10430"
     CDS             4206..6740
                     /locus_tag="VNU01_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HWH33075.1"
                     /translation="MASSTTRTQRAGGSKPSAKPSTRNAGARKTGARKTGARKPPSKP
                     ASRKAAPAKRGGRGGGRAPRDSGARDHVRDAWGAGFLLLSALSALAVYTPGAAGAIGV
                     FLDGFWRGLFGVFGLLAPPLLLGAGILLLREPKRANGRIAIGATLTLAALAGLWHLAK
                     GAPAFSAPLEDLHASAGWVGGAVALPLKAVAATWGTAVILVALLSLGLMITTATPPSA
                     VARRSGQAFTALRARVTEWWARRKLARAQGDPFDDEIFARSAQAPPKAPVEGETITLP
                     PAGDAPPPDEPPAVEEPGRTVVFEGAIDEAAGELEELSDTQLMPVGLDAGRADDTDKI
                     APLRDPSVATMLTPPDPSFAAYQLPSLDLLRTGKAAAGNKKSLDQMTRALEHTLKQFG
                     VDASVARVSRGPTVTRFEIELGPGVKVAAVTKLNDDISYALATPEIRMVAPIPGKSAI
                     GIEVPNRDRDLITLGDVLRSAEAEAAAHPLTAGIGVDIAGNAVLVNLAKMPHLLIAGA
                     TGSGKSVTMNSIVTSIITRSTPAQVRMILVDPKRVELNHYEGTPHLLTPVVTDPKRAT
                     DALAWTVKEMEDRYERLAMLGYRNIDTYNAAVRDGSVKQAPALSGKADEVWEELPYML
                     LVIDELADLMMVAPRDVESAIVRIAQMARAVGIHLIVATQRPSVDVVTGLIKANIPSR
                     IALAMATQADSRTILDQAGAEKLVGHGDMLFMPANASKPHRIQGCYVDEREIEKVVQH
                     CKRQREAEYQPGIVKSGSEAVRSDAMGDDASDEQLTRAAMELVVRSGLGSTSMLQRKL
                     KVGFARAGRLMDELETMGVVGPSEGPKARAVLMTVDELEQRLADGN"
BASE COUNT          969 a         2502 c         2489 g          916 t
ORIGIN      
        1 ggacgccgcc acgcggatga accgcagcgc ctccaccacg ccggccaggc cgcgcagggc
       61 gtcggtgaac tcggcgcgct ggtccgcgcc cacctccggc ttcagccgga acttcacgac
      121 atgccagagc acgctgcctc cgtggtgcca aaccgcatgg atgaggaggc cggaacgcta
      181 gcatcccgcg catgagcggc ccccccgagc cagcctcgac ccctgacccg gactcgacgc
      241 gctcgcgccg tcgcggcgcc gacgcgcgcc cccggcgcga ctggcgcctg cccgtgccgg
      301 acaacgtgct gctgcgccgc ttcggctacg tccgtctggt cggcttcggc gcgtggctcc
      361 tcgcctgcat cgtgctgtac gggatcttcg gcacccagat gtggccgatc gcgctggggc
      421 tgcccgtcct ggccgtggtc accggcggct acttcgcacg ctccacgcgc tacccgcgca
      481 ccgcggtggt cgtgtcgctg ctcgcggacg cgctcgtcct gggcggcgcg atcgccttcc
      541 tgggcggcag cggcagcggg ctggtgatgc tgtacgccat cgtggtggtc agcgccggca
      601 tcctgctcgg gccgggcgcc gcgaccgtgt tcggcctgct caccgtgttc ctgggcgtgc
      661 tgcaactggt catcgaggag atgggcttcc cgccggtgct gctgcaccgc ccggagctgg
      721 acgaccggct gccgatcctg ctcgccagcc tggccgggct ggtctccgtc ggctacctgt
      781 cgaccaccta cgccgggcgc ctgcacgagc tgctcgccct cgccgacgag gaggccgagc
      841 aggtgcgcga gcgcggccgc cggcgccgca gcttcgtccg gcaggcgtcc atcgacgtgc
      901 gcgagccgct gctggaggtg gagcgcgtcg ccgacgagct ggacgagcgc tgggccgaca
      961 tgaccgagtc cgagcgccgg cgcatggccg cgcgcctgcg catgcgggtc accgagctgg
     1021 acgccgagat cggccagctc gaggacctcg gcgccatgga cgagaccggc gagcagcgcc
     1081 tggtgccggt gctgctgccg cgcgccgtcg ccgacgtggt ggtcgcgctg ggcgagcgcc
     1141 tggacggcta cgtcatcgac atccaggtcc cgcagatcaa ggtgttgggc agccgtcgcg
     1201 gcgcccgccg cgtgatctac aacctcatgg agaacgtcat ccagcacacg cctctgggga
     1261 ccggcctgcg cgtcaccggg ctgaccacgg ccggccacgg cgtgctggtg ttcaccgacg
     1321 acgggcccgg catccccagc aacgtggcgc accggctgtt cgacgcgccc gacgagggcg
     1381 gcggcccgcg cgtcggtttg ccgctggtcg ccgagctgtg cgccgccatg ggcgccgaca
     1441 tccgctacga gcccgcgccc agcggcgggt cgcgcttcat ggtggcgttc aggctcgcgc
     1501 cgagcggcgc gcccagcgcg gacgacgagg ccccggcgga cgccgcggtg tccggtcccg
     1561 cggccgccga ctagactcag cggaccggcg ccgtgcccgg cgccccgcag tcaaggacgt
     1621 gagggacgtg cccatcttcg gacgagtgct caccgccatg gtcagcccgt tcaccgccga
     1681 cggcgccctg gacctggacg gcgcgcagcg cctggcggcg cacctggttg accacggcag
     1741 cgacggcatc gtggtggccg ggtccaccgg cgagtccccg acgctgaccc acgacgagac
     1801 cctggacctg gtgcgcgcgg tcaaggaggc cgtgggggac cgcgcgaagg tggtcgtggg
     1861 caccggcaag aacgacaccg cggccaccgt ggcgatgtcc cgcgaggtcg ccggcatcgg
     1921 cgtggacggg ctgctcttgg tgtcgccgta ctacaaccgg ccatcccagc gcggcatgct
     1981 cgcgcacttc ggcgcggtcg cggacgccgt cggcggcctg cccgtgctgc tgtacgacat
     2041 ccccagccgc accggctgcg agatcgccaa ggagaccatg gcgacactgg cggagcggca
     2101 cgccaacgtg gtcggcgtga aggacgccgc gggggacttc ccgaagtcgg cgtggctggt
     2161 gtcgcggacg ccggagcact tcgagctgta cgccggcgac gacgtcgcga cgctgccgct
     2221 gctggcggtc ggcggcgtcg gggtcatcag cgtggccgcg cacctggtgg gcgacgacct
     2281 cgccgccatg gtggacgcgt tccccaccga ccccggcaag gcgcgcgaga tccacttcaa
     2341 gctgctgccg ctgttctcga cgctgttctg cgacgtcaac cccgtgccgc tcaaggccgc
     2401 gctgcggctg gtcggcctgc ccggcggccc gctgcggctg cccctggccg aacccgacga
     2461 tcagacggtg cagcgcgtgc gtgaagcact cgccgccgtg ggagtcttgt ccgcgtgagt
     2521 gacccgatgc agcgcggccg ccgcgcgtcc gccagccctg tcgaagtctc gttctacgga
     2581 ggcctcgggg agatcggcgc gaacatggcg tcggtcgagg tggacggcaa gatcgcgctg
     2641 atcgacgtcg gcctgatctt cccggacgcc gagcaccacg ggatcgacct gatcctgccg
     2701 gactggtcgc ggctgcggga gcgcgccgag gacgtgcact gcgtggtgat cacgcacggg
     2761 cacgaggacc acatgggcgc gctgccgttc ttcctgcgcg acttccccgg tgtgccgatc
     2821 tactcctcgc gcctggcgct gggcctgatc ggggcgaagc tggaggagca ccccgacgtc
     2881 acgttcaccc cggtggaggt cgaggccggc gagcgcatcg agaccggccc gttcgacatc
     2941 gagttcctga gcgtcacgca ctcgatcccg gacggcatgg gcgtggcgtt ccacacgccg
     3001 cacggcacga tcctgcacac cggcgacttc aagctggacc agacgccgat cgacgggcgc
     3061 cccacggacc tgccgcactt cgcgcagctg ggcgacgagg gcgtggcgct gctgctcgcc
     3121 gactccacca acgcggaccg gcccgggcac gtcaagaccg agcgcatcgt cggcgacacg
     3181 ctgcgcgacg tgttcgccgc cgccaccggg cgcgtggtcg tcaccacgtt cgccagccat
     3241 gtccaccgcg tgcagcaggc catcgacgcc gccgtggagg tgggccgcaa ggtctgcttc
     3301 gtcggccgct ccatggtgcg caacatgccg atcgcgcgcg acctcggcta cctcaactat
     3361 cgcgacgagc acgtcgtcga gatggaggac gtcgagaaga tggcgcgcga cgccacggtg
     3421 atcgtctgca ccggctcgca gggcgagccg tacgccgcgc tgtcgctgat ggccgccgga
     3481 cagcaccgcc aggtgcgcct ggagcccggc gacacggtgg tcatggcgtc gtcggtcatc
     3541 cccggcaacg agcacgcgat cttcaagtcg atcaacgggc tgttccgcca gggcgccgac
     3601 gtggtgcaca ccggcatcgc cgacgtgcac gtgtccggcc acgcggccgc ggacgagctg
     3661 cgcatcctgc acaacatcgt ccggccgaac gccgtcatcc cggtgcacgg cgagtaccgc
     3721 cacctcgtcg cccaccgcaa gatcgcgatc gagaccggcg tggcggagga caacgtgctc
     3781 gtctgcgagg acggcgacac gatcctcctc gaggacggcg aggtgcgccg cggcgagagc
     3841 ttccaggcgg ggcgcgtctt cgtcgacggc ctcggcgtcg gcgacgtcgg caacgctgtg
     3901 ctgcgcgacc gctccaggct gtccagcgag ggtgtgtgcg tcgcggtgct ggtgaccgac
     3961 cagcacgacc gcctggacgg cgagccgatg gtggtgcagc aggggatcat ctacgagccg
     4021 gaggccggcg acctgctgga ccaggccgcc aaggtgctgg gcgaggagct ccggcgcgtc
     4081 aaggacggcg aagaagctgc ggtgcttcgc cggatcacgg tccagaccct ggcacgctat
     4141 tggaaggaac agaccgggcg gcgcccggtc atcctgccga tcgtcgtcga ggtctagagg
     4201 acatcgtggc cagcagcacc acccgcacgc agcgcgcggg cggctccaag ccgtccgcca
     4261 agccgtccac gcgcaacgcc ggcgcgcgca agaccggcgc gcgcaagacc ggcgcgcgca
     4321 agccgccgtc caagcccgcg tcgcgcaagg ccgccccggc caagcgcggc ggccgcggcg
     4381 gtggtcgcgc gccccgcgac agcggcgccc gggaccacgt tcgcgacgcc tggggcgccg
     4441 gcttcctgct gctctccgcg ctgtccgcgc tggccgtcta cacgcccggc gccgccggcg
     4501 ccatcggcgt gttcctggac ggcttctggc gcggcctgtt cggcgtgttc gggctgctgg
     4561 cgccgccgct gctgctgggc gcgggcatcc tgctgctgcg cgagccgaag cgcgcgaacg
     4621 gccgcatcgc catcggcgcg acgctgacgc tcgcggcgct ggcggggctg tggcacctcg
     4681 ccaagggcgc ccccgcgttc tcggcgccgc tggaggacct gcacgcctcc gccgggtggg
     4741 tgggcggcgc cgtggcgctg ccgctcaagg cggtggccgc cacgtggggc accgccgtca
     4801 ttctggtggc gctgctgtcg ctgggcctga tgatcaccac ggccacgccg cccagcgcgg
     4861 tggcgcgccg gtcgggtcag gcgttcacgg cgctgcgcgc gcgcgtcacc gagtggtggg
     4921 cgcgccgcaa gctcgcgcgc gcccagggcg acccgttcga cgacgagatc ttcgcgcggt
     4981 ccgcccaggc gccccccaag gcgcccgtgg agggcgagac gatcacgctg cccccggccg
     5041 gcgacgcgcc gccgcccgac gagccgcccg ccgtggagga gcccgggcgg accgtcgtgt
     5101 tcgagggcgc catcgacgag gcggctggcg agctggagga gctgagcgac acgcagctca
     5161 tgccggtggg cctggacgcc ggccgcgccg acgacaccga caagatcgcg ccgctgcgcg
     5221 acccgtccgt cgcgacgatg ctcacgccgc ccgacccgtc gttcgcggcg taccagctcc
     5281 cgtcgctgga cctgctgcgc accggcaagg ccgccgcggg caacaagaag tccctggacc
     5341 agatgacccg ggcgctggag cacacgctga agcagttcgg cgtcgacgcc tccgtggcgc
     5401 gcgtgtcgcg cgggccgacc gtcacgcgct tcgagatcga gctgggcccc ggcgtgaagg
     5461 tggccgccgt caccaagctc aacgacgaca tctcctacgc gctggccacc cccgagatcc
     5521 gcatggtggc gccgatcccc ggcaagagcg ccatcggcat cgaggtgccc aaccgcgacc
     5581 gcgacctgat cacgctgggc gacgtgctgc gctcggcgga ggccgaggcg gcggcgcacc
     5641 cgctcaccgc cgggatcggc gtggacatcg cgggcaacgc cgtgctggtg aacctcgcca
     5701 agatgcccca cctgctgatc gccggggcca ccggctccgg caagagcgtg accatgaact
     5761 ccatcgtcac gtcgatcatc acgcggtcaa cgcccgcgca ggtgcgcatg atcctggtgg
     5821 accccaagcg cgtggagctc aaccactacg agggcacgcc gcacctgctc accccggtgg
     5881 tcaccgaccc caagcgcgcg acggacgcgc tggcgtggac ggtgaaggag atggaggacc
     5941 gctacgagcg gctcgccatg ctgggctacc gcaacatcga cacgtacaac gccgcggtgc
     6001 gcgacggttc cgtgaagcag gcgcccgccc tgtccggcaa ggcggacgag gtctgggagg
     6061 agctgccgta catgctcctc gtgatcgacg agctggccga cctgatgatg gtggcgcccc
     6121 gcgacgtgga gtccgcgatc gtgcgcatcg cgcagatggc gcgcgccgtg ggcattcacc
     6181 tcatcgtggc gacgcagcgc ccgtcggtgg acgtggtgac gggcctgatc aaggcgaaca
     6241 tcccgagccg catcgcgctg gccatggcca cgcaggccga cagccgcacg atcctggacc
     6301 aggccggcgc ggagaagctg gtcggccacg gcgacatgct gttcatgccg gccaacgcat
     6361 ccaagcccca ccgcatccag ggctgctacg tcgacgagcg cgaaattgaa aaggtcgtcc
     6421 agcactgcaa gcgccagcgc gaggcggagt accagccggg catcgtgaag tccggctcgg
     6481 aggcggtgcg ctccgacgcg atgggcgacg acgccagcga cgagcagctc acgcgtgccg
     6541 cgatggaact ggtggtgcgc agcggcctgg ggtcgacctc gatgctgcag cgcaagctga
     6601 aggtgggctt cgcgcgggcg ggccggctga tggacgagct ggagacgatg ggcgtcgtgg
     6661 gccccagcga gggccccaag gcgcgcgccg tgctcatgac cgtggacgag ctggagcagc
     6721 gcctcgccga cggcaactga cagtgagaat gccgtagggc ctgcaccccg cccggtctga
     6781 ctctgcgact gacagaccgt cgtgtcctga aagggatccg tcgaccggat cggggtgcag
     6841 gttccttggg cacgagctgt cggacgaagt gacctc
//
LOCUS       DATMKC010000357         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_295613, whole genome shotgun sequence.
ACCESSION   DATMKC010000357 DATMKC010000000
VERSION     DATMKC010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295613"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VNU01_10435"
     CDS             complement(<1..1459)
                     /locus_tag="VNU01_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWH33076.1"
                     /translation="MSIAEQDLAVVQQESLQSLNPATGDVVATLPIAGPDDVRAAVAR
                     AREAAAWWRDLGFGERKRRLLAFKGVIARRSEELADLVRRENGKPVVDGMIEIITAID
                     HLDWAAKNAERVMALRKVRSSINLINHAAYVEEQPLGVIGVIGPWNYPVHTPLGSITY
                     ALAAGNAVVYKPSEYTPAIGQWLADAFASVVSEQPVFQVVHGYGQTGAALCTSGVDKV
                     AFTGSARTGRKIMAACADTLTPVLMELGGKDALIVDEDADVEAAADAAVWGALQNAGQ
                     ACVSVERAYVHEKVYDEFVQHVVDIAGKLRAGDDPLADIGPITMPSQIDIISRHLDEA
                     FAKGAKALVGGPGAVHAPYVDPVVLVDVPEDALILKEETFGPVLPIVKVTDADDAVRK
                     ANDTSYGLGNSVWGKKRAMDIARRLRSGMVAVNGIVTYAGMPSLPFGGIGESGFGRIH
                     GEDGLKEFTLTHAITKERFSLPMRLLSFQRPAKALD"
     gene            1639..>2605
                     /locus_tag="VNU01_10440"
     CDS             1639..>2605
                     /locus_tag="VNU01_10440"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWH33077.1"
                     /translation="MSQDHDFDLGIVFNHVADYLFVVRAVGERYRCLAVNDAILRLTG
                     YRREDFVGRFVDEIEPVPASMRFARHYTEALERGSPVRFEDSLDTPAGAFVVEITVTP
                     FEGADGGRYVLGVGRNISARRRAEQALRESNERLTETLESISDGFYVLDEHGRFTYVN
                     ESAERELGRSREELLGRSAPELYPESEHTDVYARYARAMATGQPVEFETYWPPRDTWY
                     AIRAFSGRHGLSVYFRDVGEQRRMEEQLRQAAKMEAVGRLAGGVAHDFNNLLSSVQGH
                     AELALARLPADGDAARHVEEVLAASERAADLVRRLMTFSRHQVLDI"
BASE COUNT          369 a          884 c          954 g          398 t
ORIGIN      
        1 ggtcgagggc tttggccggc cgctggaagc tcagcagccg catcggcagg ctgaagcgct
       61 ccttggtgat cgcgtgggtg agcgtgaact ccttgagccc gtcctcaccg tggatgcggc
      121 cgaagccgga ctcgccgatg ccgccgaacg gcaggctggg catgcccgcg tacgtgacga
      181 tgccgttcac ggcgaccatg ccggagcgca ggcgccgggc gatgtccatc gcgcgcttct
      241 tgccccacac ggagttgccg aggccatagc tggtgtcgtt ggccttgcgc acggcgtcgt
      301 cggcgtcggt gaccttcacg atgggcagca ccggcccgaa cgtctcctcc ttgaggatga
      361 gcgcgtcctc cggcacgtcc accagcacca cggggtcgac gtagggggcg tgcacggcgc
      421 cggggccgcc gaccagggcc ttggcgccct tggcgaacgc ctcgtcgagg tggcgggaga
      481 tgatgtcgat ctggctcggc atggtgatgg ggccgatgtc ggccagcggg tcgtcgccgg
      541 cgcgcagctt gccggcgatg tccacgacgt gctggacgaa ctcgtcgtag accttctcgt
      601 gcacgtaggc gcgctccacg gagacgcagg cctggccggc gttctgcagg gcgccccaca
      661 cggcggcgtc ggccgccgcc tcgacgtcgg cgtcctcgtc gacgatcagg gcgtccttgc
      721 cgccgagctc catcagcacg ggggtcaggg tgtccgcgca ggccgccatg atcttgcgcc
      781 cggtgcgggc cgagccggtg aacgcgacct tgtcgacgcc ggaggtgcac agcgccgcgc
      841 cggtctggcc gtagccgtgc accacctgga agacgggctg ctcggacacc acggaggcga
      901 acgcgtcggc aagccactgg ccgatggccg gcgtgtactc gctgggcttg tagaccacgg
      961 cgttgccggc ggccagcgcg taggtgatgg agcccagggg cgtgtggacg gggtagttcc
     1021 aagggccgat gacgccgatg acgccgagcg gctgctcctc cacgtaggcg gcgtggttga
     1081 tcaggttgat gctggagcgc accttgcgca gggccatgac gcgctcggcg ttcttggccg
     1141 cccagtccag gtggtcgatg gcggtgatga tctcgatcat cccgtccacg acgggcttgc
     1201 cgttctcgcg gcggaccagg tcggccagct cctcggagcg gcgggcgatg acgcccttga
     1261 acgccagcag ccggcgcttg cgctccccga agcccaggtc gcgccaccac gcggcggcct
     1321 cccgggcgcg cgccacggcg gcgcgcacgt cgtcgggccc ggcgatgggc aacgtcgcca
     1381 cgacgtcgcc cgtggccgga ttcagcgact gcagggactc ctgctggacg acggccagat
     1441 cctgctccgc gatcgacatg gtggctccct cgttgcgtcg gcgatgagac ggaacgcctc
     1501 ggaacgtctc atcggatcat agagggccga ccgaccgccg gtcagtcccc tttttcgcgg
     1561 gcgggggccg atgcttgtgt ccgcggcgcg cacgcctgac gatgcgacgg ggagcagggt
     1621 cggcaggcgg agacagcggt gtcacaggat cacgacttcg acctcggcat cgtgttcaac
     1681 catgtcgccg actacctctt cgtggtcaga gccgtggggg agcgctaccg ctgcctggcc
     1741 gtcaacgacg cgatcctgcg gctcaccggc taccggcgcg aggacttcgt cgggcggttc
     1801 gtggacgaga tcgaacccgt ccccgcctcc atgcgcttcg cgcgccacta cacggaggct
     1861 ctggagcgcg gcagcccggt gcgcttcgag gactccctgg acacgcccgc cggcgcgttc
     1921 gtggtggaga tcacggtcac gccgttcgag ggcgccgacg gcggtcgtta cgtgctcggc
     1981 gtcgggcgga acatctccgc gcgccggcgc gcggagcagg cgttgcgcga gtccaacgag
     2041 cggctcactg agacactcga gtcgatctcg gacggcttct acgtgctgga cgagcacggg
     2101 cggttcacct atgtgaacga aagcgccgag cgcgaactcg gccgaagccg cgaggagctc
     2161 ctcggacgct cggcgccgga gctgtatccg gagagtgagc acaccgacgt ctacgcgcgc
     2221 tacgccagag cgatggcgac cggccagccg gtcgagttcg agacgtactg gccgccgcgg
     2281 gacacctggt acgccatccg ggcgttctcc ggccgccacg gcctgtcggt gtacttccgc
     2341 gacgtcggtg agcagcggcg catggaggag cagctgcggc aggccgccaa gatggaggcc
     2401 gtgggccggc tggccggcgg ggtcgcgcac gacttcaaca atctgctgtc gagtgtccaa
     2461 ggccacgcgg agctcgcact ggcgcgcctg cctgccgacg gcgacgcggc gcggcacgtc
     2521 gaagaggtgc tggcggcgtc cgagcgcgcc gccgacctgg tgcgccggct gatgaccttc
     2581 agccggcacc aggtcctcga catcc
//
LOCUS       DATMKC010000358         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_298054, whole genome shotgun sequence.
ACCESSION   DATMKC010000358 DATMKC010000000
VERSION     DATMKC010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298054"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VNU01_10445"
     CDS             complement(<1..347)
                     /locus_tag="VNU01_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33078.1"
                     /translation="MRQIPPIIDEPPRGAGARARHWLRGRRFSLAGLLALAEVIAVLV
                     WRPGTIAASALAFVVLVLAVMGIGRVRSGLLRDLLVIVAIAQGLVLVIPILIGFSFVL
                     GLIAAAVLLVLLIG"
     gene            complement(407..1252)
                     /gene="ispE"
                     /locus_tag="VNU01_10450"
     CDS             complement(407..1252)
                     /gene="ispE"
                     /locus_tag="VNU01_10450"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWH33079.1"
                     /translation="MAGLTLPAPAKVNLVLRVAAPRPDGYHPVTTLLVAVDGLADTVR
                     VASAPARRVICPGVAERDNLAWRAMDVLERATDRPVRLRVDIDKRIPAQAGLGGGSSD
                     AAAVMRAARDLLGLALGDGDLERLAAEVGSDVPFFVRGGAQWATGRGEVLTPAEPPPG
                     LWLLLTPPVVALPTALVYRAWDAGARPPALPPPDTAAPLRDWRRDGRVGNDLWPAALA
                     RAPRLGGVARALRAAGARRVLLCGSGGALAGLFGSAEDARAAAARPGLPVLAVTCPAP
                     SGRAG"
     gene            complement(1240..2067)
                     /gene="rsmA"
                     /locus_tag="VNU01_10455"
     CDS             complement(1240..2067)
                     /gene="rsmA"
                     /locus_tag="VNU01_10455"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWH33080.1"
                     /translation="MSGAARRVNVLRLLQAHGVRPDTDLGQHFLVDENLVDLSLRAGG
                     VGPEDVVLEVGAGPGVLTAALARTARLVHAVELDRRMAPMLAEAVAGAGDVRLHWGDA
                     MRLDLAALTPPPTRLVANLPYAIATPLVVESTWRLPSLRGWCVMVQREVADRWLASRG
                     GPLYGAPSVLLQLCARPTFRRNVGREVFAPPPRVDSALVALERTGPGADGGLRALVRA
                     AFAQRRKTLANTLGAAGADRDAVRAALASLDLPPTARAEDLGPDAFRRMAEELAWPG"
BASE COUNT          282 a          880 c          852 g          184 t
ORIGIN      
        1 ccgatcagca gcaccaggag cacggcggcg gcgatgagcc cgagcacgaa cgagaaaccg
       61 atgaggatgg ggatcaccag caccagcccc tgggcgatcg cgacgatcac gaggaggtcg
      121 cgcagcaggc cggaccggac gcggccgatg cccatgacgg ccagcaccag gacgacgaac
      181 gccagggcgc tggccgcgat ggtgcccggg cgccacacga gcaccgcgat cacctcggcg
      241 agcgcgagca gcccggccag gctgaaccgg cggccgcgga gccagtggcg cgcgcgggcc
      301 ccggccccgc gcgggggctc gtcgatgatg ggcgggatct ggcgcaccgc gcggcgagtg
      361 tagcggcgcc cccccggcgg aggcctccgg tccgggtacg gcgcggtcag ccggcccggc
      421 cggacggtgc cgggcacgtc acggccagca ccgggagacc gggccgcgcc gccgccgcgc
      481 gcgcgtcctc ggcggaaccg aacagccccg cgagggcgcc gccgctgccg cacagcagca
      541 cccgccgcgc gccggcagcc cgcagcgcgc gcgcgacgcc ccccagccgg ggggcacggg
      601 cgagggccgc gggccacagg tcgttgccca cccggccgtc acggcgccag tcgcgcagcg
      661 gcgccgcggt gtccggcggc ggcagggccg gcgggcgggc accggcgtcc caggcccggt
      721 agacgagagc cgtcggcagc gcgaccaccg gcggggtgag cagcagccac agccccggcg
      781 gcggctccgc cggggtcagc acctcgcccc gtcccgtggc ccactgcgcc ccgccgcgga
      841 cgaagaacgg cacgtccgac cccacctccg cggccagccg ctccaggtcg ccgtccccga
      901 gggcgagtcc cagcagatcg cgcgcggccc gcatcaccgc cgccgcgtcg ctggacccgc
      961 cgcccagccc cgcctgcgcg gggatgcgtt tgtcgatgtc cacccgcagc cggaccgggc
     1021 ggtccgtggc gcgctccagc acgtccatcg cccgccaggc caggttgtcg cgctccgcca
     1081 cgcccgggca gatgacccgc cgcgccggcg cggaggcgac gcggacggtg tcggcgagtc
     1141 cgtcgacggc gaccaacagc gtggtgaccg ggtggtagcc gtcgggtcgt ggcgcggcca
     1201 cccgcagcac caggttcacc ttggcgggcg ccggaagcgt cagcccggcc acgccagctc
     1261 ctcggccatc cgccggaacg cgtccggccc gaggtcctcc gcccgggcgg tggggggcag
     1321 atccagggac gccagggcgg cgcggaccgc gtcccggtcg gcgccggcgg cgccgagggt
     1381 gttggcgagc gtcttgcggc gctgggcgaa ggccgcccgc acgagcgccc gcaggccgcc
     1441 gtccgcgccc ggcccggtgc gctccagcgc caccagggcc gagtcgacgc gcggcggcgg
     1501 ggcgaacacc tcgcggccca cgttgcggcg gaaggtgggc cgggcgcaca gctgcagcag
     1561 caccgacggc gcgccgtaga gcggcccccc tcgtgacgcg agccaccggt ccgcgacctc
     1621 gcgctgcacc atcacgcacc agccgcgcag cgacggcagc cgccacgtgc tctcgaccac
     1681 gagcggggtg gcgatggcat agggcaggtt ggccaccagc cgcgtcggcg gcggcgtgag
     1741 cgcggcgagg tccaggcgca tcgcgtcgcc ccagtgcagg cgcacgtccc cggcgcccgc
     1801 caccgcctcg gcgagcatcg gcgccatgcg ccggtccagc tccacggcgt gcaccagccg
     1861 cgccgtgcgc gccagggccg ccgtgagcac gcccggcccc gcgccgacct cgagcaccac
     1921 gtcctcgggc ccgaccccgc cggcccgcag cgacaggtcg acgaggttct cgtcgaccag
     1981 gaagtgctgg ccgagatcgg tgtcgggacg gacgccgtgc gcctggagga gccgcaggac
     2041 gttgacgcgc cgcgcggcgc cgctcacggc cggcggaggt cgcccgcggg cgtctcccag
     2101 ccgaagacgc gcgccgcgtt gcggtcggtg accccctcca tggcctgcgg gtcggtgccg
     2161 cgggcggcgg ccagcgccgc gagcgtgtgg gccacgag
//
LOCUS       DATMKC010000359         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_107070, whole genome shotgun sequence.
ACCESSION   DATMKC010000359 DATMKC010000000
VERSION     DATMKC010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107070"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VNU01_10460"
     CDS             <1..743
                     /locus_tag="VNU01_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWH33081.1"
                     /translation="SHAGSTPCADAEEAVGDWLSAHGVEPAWELAAPLVRAGLDEGRL
                     RALLEGLPAEAAQWLPLEAAVTEAVDDIADAASRVSGIVDAVKRYSHLDEAPVQDIDV
                     HDELERTLRVLRHKLGEGIAVVRDYAPDLPAVLGDPVALNQVWTNLIDNAVDAMGGMG
                     TLTLRTAAAGGGVEVHVGDDGPGLAPHVRDRVFEPFVTTKPVGSGTGLGLHLVWDVVT
                     RVHRGDVRVDSSQAGTVFRVWLPAAGAR"
     gene            complement(740..2731)
                     /locus_tag="VNU01_10465"
     CDS             complement(740..2731)
                     /locus_tag="VNU01_10465"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWH33082.1"
                     /translation="MTQTLEPPANALEAGRRAHERHDWSEAFDRLREAEAAAPLDADD
                     LVRLGEAAWWSGDLVSAIEARERAYALCVRQGDKARAAGLALNLVQDYASRVQHQQAG
                     AFLARAERLLTDIPESAAHGFLTLTRARFALESGDMDDALRLAREGAALARRHDDPDT
                     EALSLTIEGRVYIERGDIARGLQLLDEATTAAVTGELSPLATGIVYCSTIETCADLHD
                     FHRAGEWTEKAQEWCKRHAGENGWPGVCRVHEAEVIAFRGDWQRAEDAARLAARELAN
                     FSVDAAGAAYYEVGEVRLRMGDLAEAEHAFRLADEHARDPQPGLALLRLAQGRPDAAL
                     AAIRRALEDPGRSPLWRAKLLPASVRIGLEAGDAGLARQSAEELERLAATYGTQSPVL
                     GAQAACSRAAILLAEGQAAAAILRLREALRAWQQARAPYQAAETRARLATAYEAQQDL
                     DAAITELEAALAAFEKLGAQPDAQQAAARLRALREQAGSHARTESRVRSFLFTDIEQS
                     TALVEAIGDEAWTDLVRWHDQTLREVFAAHQGQEIDHAGDGFFVAFATADDALAAAVA
                     LQRRLADHRRDHGFAPKVRVGVHTAEAVSDAGGYRGKGVHAAARIAALAAGGEVVVSQ
                     DTLDAAGVPHPTSAPRETALPGISDAVRVATLDWMLLAD"
     gene            2852..>3178
                     /locus_tag="VNU01_10470"
     CDS             2852..>3178
                     /locus_tag="VNU01_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWH33083.1"
                     /translation="MQQADRVTVTFLFSDIEGSTKLLERLGERWPATLEAHRRVVRAA
                     LEACGGTEHGTEGDSFFASFPSAAAGVGAAALIQRGLAETAWPEGAELRVRVGVHTGE
                     AQRSGEG"
BASE COUNT          349 a         1181 c         1240 g          408 t
ORIGIN      
        1 gctcccacgc cggctcgaca ccgtgcgccg acgcggagga ggccgtgggc gactggctgt
       61 cggcgcacgg tgtcgagccg gcgtgggagc ttgcggcgcc gctggtgcgg gcggggctgg
      121 acgaggggcg cctgcgggcg ctgctggagg gcctgccggc ggaggcggcg cagtggctgc
      181 cgctggaggc ggcggtgacc gaggccgtgg acgacatcgc cgacgccgcc tcgcgggtca
      241 gcgggatcgt cgacgcggtc aagcgctaca gccatctgga cgaggcgccc gtgcaggaca
      301 tcgacgtgca cgacgagctg gagcgcacgc tgcgcgtgct gcgccacaag ctcggtgagg
      361 gcatcgccgt ggtccgcgac tacgcgccgg acctgcccgc ggtgctcggc gacccggtgg
      421 cgctgaacca ggtgtggacc aacctgatcg acaacgccgt cgacgcgatg ggcgggatgg
      481 gcacgctcac gctgcgcacg gcggccgccg gcggcggcgt ggaggtgcac gtcggcgacg
      541 acgggccggg gctcgcgccg cacgtgcgcg accgcgtgtt cgagccgttc gtcaccacca
      601 agccggtcgg ctccggaacg ggcctggggc tgcacctggt gtgggacgtg gtcacgcgcg
      661 tgcaccgcgg cgacgtgcgg gtcgactcgt cgcaggccgg gaccgtgttc cgggtgtggc
      721 tgcccgccgc cggcgcgcgc tagtcggcca ggagcatcca gtcgagcgtg gcgacgcgca
      781 cggcgtcgct gatgcccggc agggcggtct cccgcggcgc cgacgtcggg tgcggcacgc
      841 cggccgcgtc gagcgtgtcc tggctcacga ccacttcgcc cccggcggcc agcgccgcga
      901 tgcgcgcggc cgcgtgcacg cccttgccgc ggtagccgcc ggcgtcgctg accgcctccg
      961 cggtgtgcac gccgacgcgc accttgggcg cgaagccgtg gtcgcgccgg tggtccgcca
     1021 ggcgccgctg cagggcgacc gcggccgcga gggcatcgtc cgcggtggcg aaggcgacga
     1081 agaacccgtc gccggcgtgg tcgatctcct ggccctgatg cgcggcgaac acctcgcgca
     1141 gcgtctggtc gtgccagcgc accaggtcgg tccacgcctc gtccccgatc gcctccacca
     1201 gggccgtgga ctgctcgatg tcggtgaaca ggaacgagcg caccctgctc tcggtccgcg
     1261 cgtggctgcc cgcctgttcc cgcagggccc gcaggcgtgc cgcggcctgc tgcgcgtccg
     1321 gctgcgcgcc gagcttctcg aacgcggcga gggcggcctc cagctcggtg atcgccgcgt
     1381 ccaggtcctg ctgcgcctcg tacgcggtcg ccaggcgggc cctggtctcc gccgcctggt
     1441 agggcgcgcg cgcctgctgc cacgcccgca gcgcctcgcg cagccgcagg atcgcggcgg
     1501 ctgcctggcc ctcggcgagc aggatcgcgg cgcgcgagca ggcggcctgc gcgcccagga
     1561 cggggctctg ggtgccgtac gtggcggcca ggcgctccag ctcctcggca gactgccggg
     1621 ccaggcccgc gtcgccggcc tccaggccga tgcggaccga ggccggcagc agcttcgcgc
     1681 gccacagcgg cgagcggccc gggtcctcca gcgcgcggcg gatcgcggcg agcgcggcgt
     1741 cggggcggcc ctgcgccagg cgcagcaggg ccaggccggg ctgcgggtcg cgcgcgtgct
     1801 cgtcggccag gcggaacgcg tgctccgcct cggccaggtc gcccatccgc aggcgcacct
     1861 cgccgacctc gtagtaggcg gcgcccgccg cgtccacgga gaagttcgcg agctcccgcg
     1921 cggccaggcg cgcggcgtcc tcggcgcgct gccagtcgcc gcggaaggcg atgacctccg
     1981 cctcgtggac gcggcacacg ccgggccagc cgttctcgcc ggcgtggcgc ttgcaccact
     2041 cctgcgcctt ctccgtccac tcgccggcgc gatggaagtc gtgcaggtcg gcgcaggtct
     2101 cgatggtgct gcagtagacg atgcccgtgg ccagcgggct gagctcgccg gtgaccgccg
     2161 ccgtggtggc ctcgtccagc agctgcagcc cgcgagcgat gtcgccgcgc tcgatgtaga
     2221 cccggccctc gatcgtgagg ctcagcgcct cggtgtccgg gtcgtcgtgc cggcgggcga
     2281 gcgccgcgcc ctcgcgagcc agccgcagcg cgtcgtccat gtcccccgat tccagggcga
     2341 accgggcgcg cgtgagcgtg aggaagccgt gggcggcgct ttccgggatg tcggtgagca
     2401 ggcgctccgc ccgggcgagg aaggcgcccg cctgctggtg ctgcacccgc gaggcgtagt
     2461 cctggaccag gttgagcgcg agcccggcgg cgcgcgcctt gtcgccctgg cggacgcaca
     2521 gcgcgtacgc gcgctcgcgc gcctcgatcg cgctcaccag gtcgccgctc caccacgccg
     2581 cctcgccgag gcggaccagg tcgtcggcgt cgagcggcgc ggccgcctcc gcctcgcgca
     2641 gccggtcgaa cgcctcgctc cagtcgtgtc gctcgtgcgc gcggcgaccc gcctccagcg
     2701 cgttcgccgg cggctcgagc gtctgcgtca cggttcaccc ccggtccgaa ctcggtgccc
     2761 gtacggattc tcttcccgcc ccattggcag gacaaggacg aatctgcccg gggcggttag
     2821 gttgtgccga gtcggaccgg cgggagggga catgcagcag gcggatcgcg tcaccgtcac
     2881 ctttctgttc tcggacatcg agggctcgac gaagctgctg gagcggctcg gcgagcgctg
     2941 gccggccacg ctcgaagccc atcggcgcgt ggtgcgcgcc gcgctcgagg cctgcggcgg
     3001 cacggagcac ggcacggagg gcgacagctt cttcgccagc ttccccagcg ccgcggcggg
     3061 cgtcggcgcg gccgcgctga tccagcgggg cctggccgag acggcctggc ccgagggcgc
     3121 cgagctgcgc gtccgcgtgg gggtgcacac cggcgaggcg cagcggtcgg gcgagggc
//
LOCUS       DATMKC010000360         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_316931, whole genome shotgun sequence.
ACCESSION   DATMKC010000360 DATMKC010000000
VERSION     DATMKC010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316931"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2045
                     /locus_tag="VNU01_10475"
     CDS             <1..2045
                     /locus_tag="VNU01_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWH33084.1"
                     /translation="MKDRIGRLVGDRPVGVERDDEGNAKSRRWGGMWVGTFHATCARL
                     LRTEAPRLGYDKAFTIYDAADSTRLVGLCIKDLGIDPKRVTPRGAAHEISNAKNELVD
                     FDSYRLRAGENWYQEQVGEIYRLYQQRLHRASAFDFDDLLVKTVELFQLFEPVLDHYR
                     ERFAHILVDEWQDTNRAQYELVKLLALQHRNLTVVGDSDQSIYRFRGADIRNILDFQS
                     DFPDATTITLDRNYRSTQTILDAANSVIRNNTQRLPKHLWTDAGAGVKAVRYTGDNEH
                     DEAAFVAEEVERLADQHGYRYGDCAVFYRTNAQSRVLEEVLIRLGTPYQVVGGTRFYE
                     RKEVKDLLAYLRLMVNPADDVSAKRVLNVPRRGIGAKTEEALDLFAGRERVTFIEACR
                     RAEENHLLSARATGAVLDFVRLLDRLRTEAVEEELPLRQVVERAWERTGYLAELEAER
                     TVEALGRVENIKELAGVAEDFQALQPDATLAEFLERVSLVSEQDDYSDESSRLTLMTM
                     HNAKGLEFPVVFIVGMEDAVFPHHRALGDPEELEEERRLCYVAMTRARERLYLCSAWS
                     RTLFGGQSANPPSRFLKEVPQELVEDRASDRGGPSRRAVARAGARPGVLNQRRPIDVE
                     GDAEDLAVGDRVLHPTFGPGKILELTGKAGAEEALVRFDEFGTKRLVLAYAPLVRA"
BASE COUNT          346 a          787 c          780 g          305 t
ORIGIN      
        1 agatgaagga ccgcatcggc cgcctcgtgg gcgaccggcc ggtcggtgtc gagcgcgacg
       61 acgagggcaa cgcgaagtcg cgccgctggg gcggcatgtg ggtcggcacg ttccacgcca
      121 cgtgcgcgcg gctgctgcgc acggaggccc cgcgcctggg ctacgacaag gcgttcacca
      181 tctacgacgc cgcggactcc acgcggctgg tcgggctgtg catcaaggac ctcggcatcg
      241 accccaagcg ggtcaccccg cggggcgccg cgcacgagat cagcaacgcc aagaacgagc
      301 tggtcgactt cgacagctac cgcctgcgcg ccggcgagaa ctggtaccag gagcaggtgg
      361 gggagatcta ccgcctgtac cagcagcgcc tgcaccgcgc cagcgccttc gacttcgacg
      421 acctgctggt caagacggtg gagctgttcc agctgttcga gccggtcctg gaccactacc
      481 gggagcgctt cgcccacatc ctggtggacg agtggcagga caccaaccgc gcgcagtacg
      541 agctggtcaa gctgctggcg ctccagcacc gcaacctgac cgtcgtgggc gactccgacc
      601 agtcgatcta ccgcttccgc ggtgccgaca tccgcaacat cctggacttc cagtccgact
      661 tcccggacgc caccaccatc accctggacc gcaactaccg ctccacgcag accatcctgg
      721 acgcggcgaa ctcggtgatc cgcaacaaca cccagcgcct gcccaagcac ctgtggaccg
      781 acgccggcgc gggcgtgaag gccgtgcgct acaccggcga caacgagcac gacgaggccg
      841 cgttcgtcgc cgaggaggtc gagcgcctgg ccgaccagca cggctaccgc tacggcgact
      901 gcgcggtctt ctaccggacc aacgcccagt cgcgcgtgct ggaggaggtg ctcatccgcc
      961 tgggcacccc gtaccaggtc gtgggcggca cgcgcttcta cgagcgcaag gaggtcaagg
     1021 acctgctggc ctacctgcgc ctcatggtga acccggccga cgacgtgtcc gccaagcgcg
     1081 tgctcaacgt cccgcggcgc ggcatcggcg ccaagaccga ggaggcgctg gacctgttcg
     1141 ccggccgcga gcgcgtcacg ttcatcgagg cgtgccgccg ggcggaggag aaccatctgc
     1201 tgtcggcgcg cgccaccggc gccgtgctcg acttcgtgcg gctgctggac cggctgcgga
     1261 ccgaggcggt cgaggaggag ctgccgctgc gccaggtcgt ggagcgcgcc tgggagcgca
     1321 ccggctacct ggccgagctg gaggcggagc gcacggtgga ggcgctgggg cgcgtggaga
     1381 acatcaagga gctggcgggc gtcgccgagg acttccaggc gctgcagccc gacgccaccc
     1441 tggcggagtt cctggagcgg gtgagcctgg tcagcgagca ggacgactac agcgacgaga
     1501 gctcgcgcct cacgctcatg accatgcaca acgccaaggg gctggagttc cccgtggtgt
     1561 tcatcgtcgg catggaggac gcggtgttcc cgcaccaccg ggcgctcggc gacccggagg
     1621 agctggagga ggagcgccgc ctgtgctacg tcgccatgac gcgcgcgcgt gagcggctgt
     1681 acctgtgcag tgcctggagc cgcacgctgt tcggcggcca gagcgccaac ccgccgtcgc
     1741 gcttcctcaa ggaggtgccg caggagctgg tggaggaccg cgcctccgac cggggcggtc
     1801 ccagccgccg cgccgtggcg cgcgccggcg cgcggccggg cgtgctcaac cagcgccgcc
     1861 ccatcgacgt ggagggggac gccgaggacc tggccgtcgg cgaccgcgtg ctgcacccga
     1921 cgttcggccc gggcaagatc ctggagctga ccggcaaggc cggcgccgaa gaggcgctgg
     1981 tgcgcttcga cgagttcggg accaagcggc tggtgctcgc gtacgcgccc ctggtgcgcg
     2041 catgagctgt ccctttcgcc ccctcaagta agcctcggcc cgggtcgacg ttgtagcggc
     2101 aagacccata tcgtcccggc acacgctcag ctcttggggg tcagacccat gtctccttcg
     2161 atctggcagc gcttcgcgtt gcccgtcgcg ctcctgttcg ccgccgtctc ctgcggag
//
LOCUS       DATMKC010000361        10636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_247333, whole genome shotgun sequence.
ACCESSION   DATMKC010000361 DATMKC010000000
VERSION     DATMKC010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247333"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VNU01_10480"
     CDS             complement(<1..1124)
                     /locus_tag="VNU01_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWH33085.1"
                     /translation="MSVVTDVFERLRRDLLRQVEEEGLDPHADAEAIRARAADAVAAY
                     QRGAHLGEGAPLHDPEDTTRRLVDALTRYGALTDLLERRDVEEVFIEGGQVRFIAGDG
                     RIRTLAAPTTEEENRQIVERLLALTPRRLDTASPLTQARVLGGTARLTAAIPPVSERL
                     SATIRRHTLRRDTLASLSAAGAMEPAPAAFLWGVMQGQVRLLVSGPPGAGKTSLLGAL
                     LRAVPGDRCIRCCEEIRELTVPLSHGAYYETRPPGLDGGAEISLRDLVKFVLAMRPDL
                     IVVGEVRGAEAFELTRAANAGCGMACTIHANHARAALDALVHAAVMAGENVTEQALRA
                     VFAAGVDLVVHCELRQAGDSDRAARRRVSEIIAVVPSLHGG"
     gene            complement(1121..2236)
                     /locus_tag="VNU01_10485"
     CDS             complement(1121..2236)
                     /locus_tag="VNU01_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33086.1"
                     /translation="MSAVPVLALAATPRDWAARLHRHVADHGGGRIRATVLTAAEALA
                     ERYDVLLADDRTSFLSQRLIRSLHDSGRLVVGLYDAADPQGKSDLVDWGVDEALPSDL
                     APGELLAAIQELLPSRPAERRHPGGQPVAPAAVPAVSRLAGAIVVRGPGGGAGRTEVA
                     LGLAAELAITGGSTVLVELDEARASLAPRLGLPPYPNLRAAVDWSRRGAGSARAAVQR
                     ADAGLGVLVAGPVPQSLGPAPGDDLAAVLSDVARGADWVVVDAGEAGAPLPIATTVLV
                     GAATPVGAVRLTALLAAQADAAHVVVNRAPASAYRRWEFLDELRRAHTPLSVTTVPED
                     RNVFDASWRGRPSQRGPFARAMRALAQRIAAERETAA"
     gene            complement(2233..2952)
                     /gene="cpaB"
                     /locus_tag="VNU01_10490"
     CDS             complement(2233..2952)
                     /gene="cpaB"
                     /locus_tag="VNU01_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWH33087.1"
                     /translation="MSDNVQVAAPIAAPASAAPGAALRRPWWSRLSRAHVAALAAAVV
                     AGGLNLMALQRGDARVPMLVAAADLGHGAALDPAMVRAVPMLGGPGALDGLVPMSRLR
                     DRQGWLLTRRVHAGEPLRWSDLRDPRELGGMRAMSLPIQPEHAAGGALRPGDRVDVIA
                     VREGRAMWVAAGVEVLDVEDLAAEGLGNVRAYSVTVAVTEAAALRLAWALHDGALELI
                     RATGAPLLSEPGTVVADEAAA"
     gene            complement(3248..4114)
                     /locus_tag="VNU01_10495"
     CDS             complement(3248..4114)
                     /locus_tag="VNU01_10495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH33088.1"
                     /translation="MHHSSLQSLLRHRASLVPLVATIVGLALGLAIHTTAGATAAGRA
                     DHDRPALQHRSLGTGQVAEARLRQGGERSSRAVRGAVTTRPAPVTPPTPPAPAPRAPE
                     ESGLRAPAGASRAFAGVEHLTLSLPQGARAVGFHESGSGRALSMHPMGIPRVNGNMTR
                     LSSAPVGDGFEYLVLPSRGRRNGPTSAVDVSMPFNWPVQAPVTGTVVDATSYLLYGNT
                     PDNAVYIVPDSRPDLVLVAMHLNGLKINVGDRVEAGKTVLALTARQLPFGSQIDRYAG
                     RLPHVHMELRPR"
     gene            4482..4754
                     /locus_tag="VNU01_10500"
     CDS             4482..4754
                     /locus_tag="VNU01_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33089.1"
                     /translation="MDRARSNPEAEAVDGRHDELALNLVAEACVAVELASAEDERTLA
                     RLESLRAELLSVAYCCGDEAAREALLAVELIQDALDVQPVPAALAS"
     gene            4800..5321
                     /gene="tadA"
                     /locus_tag="VNU01_10505"
     CDS             4800..5321
                     /gene="tadA"
                     /locus_tag="VNU01_10505"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWH33090.1"
                     /translation="MAQLTHRIWMERALEQARLAEAHGDVPVGAVVVKDGAVLAEGRN
                     VRERDLDPTGHAELVAVREAARALGSWRLDGCVLYVTLEPCTMCAGALVLARLPLLVF
                     GAADPKAGAVGSLYNLVQEPRLNHRVDVVGGVLADDSGALLRRFFQARRARGESPPPG
                     GAATLPGVPGGVA"
     gene            5307..5395
                     /locus_tag="VNU01_10510"
     tRNA            5307..5395
                     /locus_tag="VNU01_10510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5343..5345,aa:Ser,seq:cga)
     gene            complement(5471..5557)
                     /locus_tag="VNU01_10515"
     tRNA            complement(5471..5557)
                     /locus_tag="VNU01_10515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5519..5521),aa:Ser,seq:gga)
     gene            5679..6737
                     /locus_tag="VNU01_10520"
     CDS             5679..6737
                     /locus_tag="VNU01_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWH33091.1"
                     /translation="MRVLGVPVRVDGSLLLIAGLVAWFYADVFGGRPGAPAGAALGVG
                     AALAAAGFVLSLLAHELGHAVTSRAMGIPVHGITLFLMGGVTESAEEAKRPRDDFLIV
                     ALGPVLSGLAGLLAFAVARLDPPQPLDSVAVFLAWANWVLAVFNLVPGYPLDGGRLLR
                     AVLWGVTGRPHAATRWAARIGQGFAGLLGAYGLWALTQGAGGLGGLWEIFIAVFLWRG
                     AAQSHARAGARERLAAKTVRDVMGSVPPSLDPSWSLAEALERIQEKPSLLWPVGDPVI
                     GGLTLAEVDAVEDLRWHDVTVGDAALRGDSALVAADAGLEEALRRMRDAPHTMLVVVE
                     DGRAVGLLTPSLLGFGDE"
     gene            6734..7756
                     /locus_tag="VNU01_10525"
     CDS             6734..7756
                     /locus_tag="VNU01_10525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HWH33092.1"
                     /translation="MRRSLRTIAAPLAAAVLMVLAFRAPLPYFTERPGALFGLDGRVA
                     VQGHPEPLHGDVLFGTVNVRGATLVAAVRAALERDVQLVRRERLIPEGLDSQAFFEQQ
                     QAEFALAADVAAAVGLRAAGLPVEPDALRGEGALVLRVVRGGPAQRRLRPGDVVTAVN
                     ARPVTTDGEYHAVVDALAWEPMTLTVQRDGVVQDIAITPAATRAGRPSVGLVVSTFRP
                     QVSLPVPVRLSDIRVGGPSAGLAIALTVYDRVAAEDLLAGRVVAATGTLDTRGRVGPI
                     GGVGLKAIAADRAAVDVLLVPAEQLEQARQAGGDTRVRIVGVATFDEALEALREAAAR
                     RPRAAA"
     gene            7829..7928
                     /gene="ffs"
                     /locus_tag="VNU01_10530"
     ncRNA           7829..7928
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNU01_10530"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8019..9785
                     /gene="dnaX"
                     /locus_tag="VNU01_10535"
     CDS             8019..9785
                     /gene="dnaX"
                     /locus_tag="VNU01_10535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWH33093.1"
                     /translation="MAYVSLYRKYRPQTFSEIVGQEHVSRTLSQAVTEDRLHHAYLFT
                     GPRGTGKTSTARILAKAINCEQGPTATPCNACSQCLGITTGSSVDVIEIDAASHGGVD
                     DVRDLRDRVAFAPASARMKVYIVDECHMLSTAGWNAFLKTVEEPPGHVLFVFATTEPH
                     KVLATILSRTQRFDFKRVPADLLAGHVRDLCGREGVTIDPDAVELVVRAGDGSVRDTL
                     SVLDQVMAFTGSSVTAAAVADVLGVTPAELLGEAADALAAHDAAGVMTLVQRIAEAGL
                     DARQFARDLVEHLRSLFLLQVAPDAGLVETTPERLVELRAQSERLGRHELLRAMDLLA
                     DAQALMRRGNTRLPLELALAKAAMPEHSGDAAALAARLDRLERSGAAAAPAPPGRVQV
                     GPDNAQSAPISGPELGTTEEADTSTAEEPSRVQAEHDSAQDAPIQGGADLGAVLARWD
                     EVMDHVKARKIRFHAMLQMGRPLALNGNVLTLEFRDGYSLHADQCASPQGAEVIAAAV
                     QDVLGFSPAIRTVVGGADAGPAPAVHAADEAEHEAQQMAEVMETEDAERHGELPDDAE
                     AEALAVQALTTHLGATVVEDAD"
     gene            9821..10135
                     /locus_tag="VNU01_10540"
     CDS             9821..10135
                     /locus_tag="VNU01_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWH33094.1"
                     /translation="MSNMNQVMRQAQAMQRKLKKAQDELAAMEFSGSAGGGMVKATVS
                     GDGRLSAVQIDKAAVDPDDVELLQDMVVAAVTEAMRSRTEAEERLLGPIAGGLGVPGL
                     GL"
     gene            10138..>10636
                     /gene="recR"
                     /locus_tag="VNU01_10545"
     CDS             10138..>10636
                     /gene="recR"
                     /locus_tag="VNU01_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWH33095.1"
                     /translation="MLYEGPVQSLVEEFGRLPGIGPKSAQRLAFHVLNADAADAERLA
                     RAILEVKEKVRFCARCFNVSELEECRICRDARRDATVICVVEEPRDVLAIERTGEFRG
                     RYHVLGGAISPIDGVGPDQLRIKELAARIGAEDVAEVVLATNPNIEGEATASYLARLL
                     KAMDLK"
BASE COUNT         1327 a         3870 c         4118 g         1321 t
ORIGIN      
        1 ccgccgtgca gggacgggac cacggcgatg atctcgctca cgcgccgccg ggcggcacgg
       61 tcgctgtcac ccgcctggcg cagctcgcag tgcaccacca ggtcgacgcc ggccgcgaag
      121 accgctcgca gcgcctgctc ggtgacgttc tcccccgcca tgaccgccgc gtggaccagg
      181 gcgtccagcg cggcgcgcgc gtggttggcg tggatggtgc acgccatgcc gcagcccgcg
      241 ttggccgcgc gtgtgagctc gaaggcctcc gcgccgcgaa cctctcccac cacgatgagg
      301 tcagggcgca tggccaggac gaacttcacc aggtcgcgca ggctgatctc cgcgccgccg
      361 tccaggccgg gcggccgggt ctcgtagtag gcgccgtggc tcaacggcac ggtgagctcg
      421 cggatctcct cgcagcagcg gatgcagcgg tcgcccggca cggcgcgcag cagcgcgccc
      481 agcagcgagg tcttgccggc gcccggcggg ccggacacca gcaggcgcac ctgaccctgc
      541 atcacgcccc acaggaacgc cgcgggggca ggctccatgg cgccggccgc cgagagggag
      601 gccagcgtgt cgcgccggag ggtgtgccgg cggatcgtgg cggacagccg ctcgctgacg
      661 ggggggatcg ccgcggtgag ccgcgcggtg ccgcccagga cgcgcgcctg ggtcagcggg
      721 ctggcggtgt ccaggcgccg cggggtcagg gccagcagcc gctccacgat ctgccggttc
      781 tcctcctcgg tggtgggcgc cgccagcgtg cggatgcgcc cgtcgccggc gatgaagcgg
      841 acttgcccgc cttcgatgaa cacctcttcg acgtcccggc gctccagcag gtcggtgagc
      901 gccccgtagc gggtgagcgc gtcgacgagg cgccgggtgg tgtcctccgg gtcgtgcagc
      961 ggcgcgccct cgcccaggtg ggcgccgcgc tggtaggcgg cgacggcgtc cgcggcccgc
     1021 gcccggatcg cctccgcgtc ggcgtgcggg tcaagtcctt cctcctcgac ctggcgcagc
     1081 aggtcgcgcc gcagccgctc gaagacgtcc gtcaccacgc tcatgcggcc gtctcccgct
     1141 ccgcggcgat ccgctgggcc agcgcgcgca tggcccgcgc gaacgggccg cgctgcgatg
     1201 ggcggccgcg ccaggacgcg tcgaagacgt tgcgatcctc tgggacggtc gtgaccgaaa
     1261 gaggcgtgtg ggcgcggcgc aactcgtcca ggaactccca gcgccggtag gccgaggcgg
     1321 gcgcccggtt cacgaccaca tgggcggcgt cggcctgcgc ggcgagcagg gcggtcaggc
     1381 ggacggcgcc gaccggcgtg gcggcgccga ccagcaccgt cgtcgcgatc ggcagcggcg
     1441 cgccggcctc tccggcgtcg accaccaccc agtccgcccc gcgcgcgacg tcggacagca
     1501 ccgcggccag gtcgtcgccc ggtgccggcc ccagggactg cggcaccggg ccggccacga
     1561 gcacgcccag gcccgcgtcc gcgcgctgga cggccgcccg cgccgacccg gcgccacggc
     1621 ggctccagtc gaccgccgca cgcaggttcg ggtagggcgg caggcccaga cgcggcgcga
     1681 gggaagcccg cgcctcgtcg agctcgacca ggaccgtgct gccgcccgtg atggccagct
     1741 cggccgccag tcccagcgcc acctcggtgc ggccggcgcc gccaccgggc ccgcggacga
     1801 cgatggcgcc cgccaggcga gacaccgccg ggacagccgc gggagccacc ggctgaccgc
     1861 ccgggtggcg ccgctccgca ggccggctgg ggaggagctc ctggatggcg gccagcagct
     1921 cgccgggcgc aagatccgac ggcagcgcct cgtcgacccc ccagtccacc agatcgctct
     1981 tgccctgcgg gtcggcggcg tcgtacagcc ccacgaccag ccggccgctg tcgtgcagcg
     2041 accggatcag ccgctggctg agaaacgacg tgcggtcgtc cgccaggagc acgtcgtagc
     2101 gctcggcgag ggcttccgcc gcggtgagca ccgtggcgcg gatgcgcccg ccaccgtggt
     2161 cggcgacgtg acggtgcagg cgcgccgccc agtcgcgagg cgtggccgcg agggcgagta
     2221 cggggacggc gctcacgccg ccgcctcgtc tgccaccacc gtgcccggct cgctcagcag
     2281 cggcgcgccg gtcgcccgga tcagctcgag cgcgccgtcg tgcagcgccc aggcgagccg
     2341 gagcgccgcc gcctcggtca ccgcgacggt gaccgagtag gcgcgcacgt tccccagtcc
     2401 ctccgccgcc aggtcctcca cgtccaggac ctccacgccg gcggccaccc acatggcgcg
     2461 gccctcccgg acggcgatca cgtccacccg gtcgccgggg cgcagcgcgc cgccggccgc
     2521 gtgctccggc tggatcggca ggctcatggc gcgcatgccg ccgagttcgc gcgggtcccg
     2581 caggtcgctc caccgcagcg gctcaccggc gtgcacccgc cgggtcagca gccagccctg
     2641 ccggtcccgg agccggctca tggggacgag cccgtcgagc gcgcccgggc cgcccagcat
     2701 ggggacggcg cgaaccatcg cggggtccag tgccgccccg tgaccgagat ccgcggcggc
     2761 cacgagcatg ggcacgcggg cgtcgccgcg ttgcagcgcc atcaggttca gcccgccggc
     2821 cacgactgcc gcggccagcg ccgcgacatg cgcgcgcgac agcctgctcc accacggtct
     2881 gcgcaaggcc gcgccgggcg cggccgaggc cggcgccgcg atcggcgcgg ccacctggac
     2941 gttgtcggac atgcccgccc ccgttcgtcc gcgaacaaac gggcgggacc ttagtgccgg
     3001 ccgcggcggg cggcaacacc cgatcgggtg aatctgtgga cgaactcagg ggagcgccgt
     3061 acgcgccgcc acgggcccgt tcctcagggg cgctgcccgc ctccgaccgg ttcgtccggc
     3121 cgcatcatga cggcatgtcg gacgtgtccg gcggcggcgc gtgtacgcac cggacacaac
     3181 aacgccgggg ccatgccacc ccggcgtcgt gtcccctcgt cctggcctgc accaggaaag
     3241 gtatccgcta tcgcgggcgc aactccatat gcacgtgcgg gagccggccg gcgtagcggt
     3301 cgatctggct gccgaacggc agttgccgtg cggtcagcgc caggacggtc ttgccggcct
     3361 ccacgcggtc gcccacgttg atcttcagcc cgttcaggtg catggccacc agcacgaggt
     3421 ccggccggct gtcggggacg atgtagaccg cgttgtcggg cgtgttgccg tacagcaggt
     3481 aggaggtcgc gtccacgacg gtgccggtga ccggcgcctg cacgggccag ttgaacggca
     3541 tcgagacgtc caccgcgctg gtggggccgt tgcggcggcc gcgcgagggc agcaccaggt
     3601 actcgaagcc gtcgccgacc ggcgcgctgg acaggcgggt catgttgccg ttcacccgcg
     3661 ggatgcccat cgggtgcatg gacagggcgc ggccggagcc ggactcgtgg aagccgacgg
     3721 cgcgcgcgcc ctgcggcagg ctcagggtca ggtgctccac gccggcgaag gcgcgggagg
     3781 cgccggcggg ggcccgcagg ccggactcct ccggcgcccg cggcgcgggc gccggaggcg
     3841 tgggcggggt gacgggggcc ggccgggtcg tgaccgcgcc gcgcacggcg cgcgagctgc
     3901 gctcgccgcc ctggcgcagc cgcgcctcgg caacctgccc ggtgcccagg ctgcggtgct
     3961 gcagtgccgg gcggtcgtgg tcggcgcggc cggcggcggt ggcgcccgcg gtggtgtgga
     4021 tggccaggcc cagggccagg ccgacgatcg tggccacgag ggggaccagc gacgcccggt
     4081 ggcggagcag ggactgcagg gagctgtggt gcattcgtct cgccttgtcg ttgggcggcc
     4141 gcggcggcgg ctgccgttca ggcacgcggc gccgagcccc ggctccaagc ggcggctgcc
     4201 gcggggcaag gtcagggcca cgcgcgcacc ggccgtcccc acaaggcagg ccgatgctgg
     4261 atatgtggac gagctcgcgc ccggactggc tggccggccg cggtcccccg gcgccgggcc
     4321 tggcccggtg cccgcagggg gactcaccgc cccccgggaa ggggacagcg ctgcaacggt
     4381 aggtgtcggg ggcatcacgg gcaggcgcgg gcggcaatct tgacggtgcc gcccgcggcg
     4441 cctccggggc cctgtcacac ccatgtggca tggtcgcctg gatggatcga gcgcggagca
     4501 atccggaggc ggaagctgtg gacggacggc acgacgagct ggcgctgaac ctggtggccg
     4561 aggcctgcgt ggcggtggag ctggcgtccg cggaggacga gcgaacgctc gcgcgcctgg
     4621 agtccctgcg cgccgagttg ctgtcggtgg cgtactgctg cggtgacgag gccgcgcgcg
     4681 aggcgctgct cgcggtcgag ctcatccagg acgcgctcga cgtgcagccg gtgccggccg
     4741 ccctggcctc gtaggggcct ccggcggccg cgccacgcgg cgtctggcag gctggggccg
     4801 tggcccaact cacgcaccgc atctggatgg aacgcgcgct ggagcaggcg cgcctggccg
     4861 aagcccacgg cgacgtgccg gtgggcgcgg tcgtggtcaa ggacggtgcc gtgctcgccg
     4921 aggggcgcaa cgtccgtgag cgcgacctgg accccacggg ccacgcggag ctggtcgccg
     4981 tgcgcgaggc cgcccgggcg ctgggctcgt ggcggctgga cggctgcgtg ctctacgtga
     5041 cgctggagcc gtgcaccatg tgcgccggcg cgctggtcct ggcgcgtctg ccgctgctgg
     5101 tgttcggggc ggccgacccg aaggccggcg cggtcgggtc gctgtacaac ctggtgcagg
     5161 agccgcgcct gaaccaccga gtggacgtgg tcggcggcgt gctggccgac gacagcggcg
     5221 ccctgctgcg gcggttcttc caggccaggc gcgcgcgggg cgagtcgccg ccacccggcg
     5281 gcgctgctac ccttcccggc gttccgggtg gagtcgcata gtccggccta gtgcgcggca
     5341 ctcgaaatgc cgtatggggg caaccccatc gtgggttcga atcccacctc caccacccga
     5401 cggcccgcga cgcggaccga cgcacggaaa aggcccaccg cctgcgcgac ggtgggcctt
     5461 tggcgttgcg cggagtcggc gagattcgaa ctcgcgaggg gttgccccct acggccattc
     5521 caagaccgcg ccataggcca gactaggcga cgactccgtg cggccgaacg gccacgcggc
     5581 gagtatagcc agcgaccggt gggcttcagc ccggcccggc ggggcacggc gaggcctgcc
     5641 cgcgcgaggc gcgagccgcg agaacgacag gagagacgtt gcgggttctg ggcgtgccgg
     5701 tccgggtcga tggctccctg ctgctgatcg cgggcctggt cgcatggttc tacgccgacg
     5761 tgttcggcgg ccgcccgggc gcgccggccg gcgcggcgct gggcgtcggc gcggcgcttg
     5821 ccgcggccgg gttcgtgctc agcctgctgg cccacgagct cggccacgcc gtgaccagcc
     5881 gcgccatggg catccccgtg cacggcatca cgctgttcct catgggcggc gtcacggagt
     5941 ccgcggagga ggccaagcgc ccccgcgacg acttcctgat cgtggcgctg ggcccggtgc
     6001 tgagcgggct ggccgggctg ctcgccttcg ctgtggcgcg cctggacccg ccgcagccgc
     6061 tggacagcgt ggcggtgttc ctcgcctggg ccaactgggt gctggccgtg ttcaacctgg
     6121 tgcccgggta tccgctggac ggcgggcggc tgctgcgagc ggtgctgtgg ggcgtcaccg
     6181 gccggcccca cgcggcgacg cgctgggccg cgcggatcgg ccagggcttc gcgggcctgc
     6241 tcggcgccta cgggctgtgg gcgctcacgc agggcgccgg cgggctgggc gggctgtggg
     6301 agatcttcat cgccgtgttc ctctggcgcg gcgcggcaca gtcccacgcc cgcgcgggcg
     6361 cgcgggagcg cctggccgcc aagaccgtgc gcgacgtcat gggctccgtg ccgccgtcgc
     6421 tggacccgtc ctggagcctt gccgaggcgc tcgagcggat ccaggagaag ccgtcgctgc
     6481 tctggccggt gggcgacccg gtgatcggcg ggctcacgct ggccgaggtc gacgccgtgg
     6541 aggacctgcg ctggcatgac gtgacggtcg gtgacgcggc gctgcgcggg gacagcgcgc
     6601 tggtcgcggc cgacgccggc ttggaggagg cgctgcgccg catgcgcgac gcgccgcaca
     6661 cgatgctcgt ggtcgtggag gacgggcgcg ccgtggggct gctcactccc agcctgctgg
     6721 ggttcgggga cgagtgaggc ggtcgctgcg gacgatcgcg gcgccgctgg ccgccgccgt
     6781 gttgatggtg ctggcgttcc gcgcgccgct gccctacttc accgagcggc ccggggccct
     6841 gttcggcctg gacgggcgcg tggcggtgca aggccatccc gagccgctgc acggcgacgt
     6901 gctgttcggc accgtgaacg tgcggggcgc gacgctggtc gccgcggtgc gcgcggcgct
     6961 ggagcgcgac gtgcaactgg tgcgccgcga gcggctgatc cccgaggggc tggactcgca
     7021 ggctttcttc gagcagcagc aggccgagtt cgcgctggcc gccgacgtcg ccgccgcggt
     7081 cgggttgcgg gcggccggcc tgcccgtgga gccggacgcg ctgcggggcg agggcgcgct
     7141 ggtgctgcgg gtggtgcgcg gcggccccgc gcagcgccgg ctgcgtcccg gcgacgtggt
     7201 gaccgccgtc aacgcccgcc cggtcaccac ggacggcgag taccacgccg tcgtggacgc
     7261 gctcgcgtgg gagcccatga cgctgaccgt gcagcgggac ggggtggtcc aggacatcgc
     7321 catcacgccc gccgccacgc gcgcgggccg gcccagcgtg gggctggtgg tctcgacctt
     7381 ccggccgcag gtgtcgctgc cggtgcccgt ccgcctgtcc gacatccgcg tcggcgggcc
     7441 cagcgcgggc ctggcgatcg cgctcacggt ctatgaccgc gtcgccgcgg aggacctgct
     7501 cgccgggcgg gtggtggccg ccaccggcac cttggacacc cggggccgcg tgggccccat
     7561 cggcggggtc gggctcaaag ccatcgcggc ggaccgggcc gcggtggacg tgctgctggt
     7621 ccccgccgag cagctcgagc aggcacgcca ggcgggcggg gacacgcgcg tgcgcatcgt
     7681 cggtgtcgcc acgttcgacg aggcgctcga ggccttgcgg gaggcggcgg cgcggcgtcc
     7741 gcgggcggcc gcctgaggcg gctctgcccg cgtcgcgccc gctccctata ctggggcgtc
     7801 cgccggtggc agcgccgggc gggtggacgg cgaggtcccg tgcgacgtcg cgccgtgaac
     7861 ctggtcaggg ccggaaggca gcagccataa gcggtccgcg acgggtgccg cggtcaagcc
     7921 gcgtcgcttc gcccgtccgg cactgtcccc ggcgcactcc ttccccgccc gacagcgccc
     7981 ggccggagcg acccggcatt catcgacagg acgccgacgt ggcctacgtc tcgctgtacc
     8041 gcaagtaccg cccgcagacg ttctccgaga tcgtcgggca ggagcacgtg tcccggacgc
     8101 tgtcgcaggc ggtgaccgag gaccgtctgc accacgcgta cctgttcacc ggcccgcgcg
     8161 ggaccggcaa gacctcgacc gcccgcatcc tggccaaggc gatcaactgc gagcaggggc
     8221 ccaccgccac gccgtgcaac gcgtgcagtc agtgcctggg catcaccacc gggtcctcgg
     8281 tcgacgtgat cgagatcgac gcggcctccc acggcggcgt ggacgacgtg cgcgacctgc
     8341 gcgaccgcgt cgcgttcgcc cccgcctcgg cgcgcatgaa ggtctacatc gtcgacgagt
     8401 gccacatgct gtccacggcc ggctggaacg cgttcctcaa gaccgtggag gagccgcccg
     8461 gccacgtgct gttcgtcttc gccacgaccg agccgcacaa ggtcctggcc acgatcctgt
     8521 cacgcacgca gcgcttcgac ttcaagcgcg tcccggcgga cctgctggcc ggccacgtgc
     8581 gcgatctgtg cggccgtgag ggcgtgacca tcgacccgga cgcggtggaa ctggtggtgc
     8641 gcgcgggcga cggcagcgtc cgcgacacgc tgtccgtgct cgaccaggtg atggcgttca
     8701 cgggctcgtc ggtcacggcg gcggcggtgg cggacgtgct gggtgtcacg cccgccgaat
     8761 tgctgggcga ggccgccgac gcgctggcgg cgcacgacgc ggcgggcgtg atgacgctgg
     8821 tgcagcgcat cgccgaggcc gggttggacg cgcgccagtt cgcgcgcgac ctggtggagc
     8881 acctgcgctc gctgttcctg ctgcaggtgg ccccggacgc ggggctggtg gagaccacgc
     8941 ccgagcgcct ggtggagctg cgggcgcagt cggagcgcct cggccggcac gagctgctgc
     9001 gcgccatgga cctgctcgcc gacgcgcagg cgctgatgcg ccggggcaac acccgcctgc
     9061 cgctggagct ggcactggcg aaggcggcca tgcccgagca cagcggggac gcggcggcgc
     9121 tggccgcccg cctggaccgc ctcgaacgct ccggcgccgc agcagcgcct gcccccccag
     9181 gtcgggtgca ggtcgggcca gataatgccc aaagtgcacc catatcgggg ccggagctgg
     9241 ggacaaccga ggaagccgac acatccacag ctgaagagcc gagccgggtg caggctgagc
     9301 acgatagtgc ccaagatgca cccatccagg ggggtgcaga cctcggcgcg gtgctggcgc
     9361 gctgggacga ggtcatggac cacgtcaagg cccgcaagat ccgcttccac gcgatgctgc
     9421 agatgggccg gccgctcgca ctgaacggca acgtgctcac cctggagttc cgcgacggct
     9481 acagcctgca cgccgaccag tgcgcctccc cgcagggcgc cgaggtgatc gcggctgccg
     9541 tccaggacgt gctgggcttc agcccggcga tccgcaccgt ggtgggagga gccgacgccg
     9601 ggcccgcgcc ggcggtccac gccgccgacg aggccgagca cgaggcgcag cagatggccg
     9661 aagtgatgga gaccgaggac gcggagcgcc acggggagct gcccgacgac gccgaggccg
     9721 aggcgctcgc cgtgcaggcg ctcaccaccc acctcggcgc gaccgtcgtg gaggacgcgg
     9781 actagcccgg gccgccgggg cacgcgggta ccctggcgcc atgtcgaaca tgaaccaggt
     9841 catgcgccag gcgcaggcca tgcagcgcaa gctcaagaag gcccaggacg agcttgcggc
     9901 gatggagttc tccggctccg cgggtggcgg catggtcaag gccaccgtgt ccggcgacgg
     9961 ccggctcagc gccgtgcaga tcgacaaggc ggccgtcgac cccgacgacg tggaactgct
    10021 ccaggacatg gtggtggccg ccgtcacgga ggcgatgcgc tcccggaccg aggcggagga
    10081 acggctgctc gggccgatcg ccggcggcct gggcgtcccc ggcctgggac tgtagggatg
    10141 ctgtacgagg gcccggtcca gagcctggtc gaggagttcg gcaggctgcc cggcatcggc
    10201 cccaagagcg cgcagcgcct ggccttccat gtgctcaacg ccgacgcggc cgacgccgag
    10261 cgtctggcca gggccatcct cgaggtgaag gagaaggtcc gcttctgtgc gcgctgcttc
    10321 aacgtcagcg agctggagga gtgccgcatc tgccgcgatg cccgccgcga cgccacggtc
    10381 atctgcgtcg tggaggagcc gcgcgacgtc ctggcgatcg agcgcaccgg cgagttccgc
    10441 ggccgctatc acgtcctggg tggggccatc tcaccgatcg acggggtggg gccggaccag
    10501 ctgcgcatca aggagctcgc cgcgcgcatc ggtgcggagg acgtcgccga ggtggtgctc
    10561 gccacgaacc cgaacatcga gggcgaggcc acggccagct acctggcgcg gctgctcaag
    10621 gccatggacc tcaagg
//
LOCUS       DATMKC010000362         5020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_338590, whole genome shotgun sequence.
ACCESSION   DATMKC010000362 DATMKC010000000
VERSION     DATMKC010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338590"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /gene="groL"
                     /locus_tag="VNU01_10550"
     CDS             <1..1474
                     /gene="groL"
                     /locus_tag="VNU01_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWH33096.1"
                     /translation="VAKEIELDNPFANMGAQLLKEAATKTNDVAGDGTTTATVLAQAM
                     VREGMRNVAAGANPMLLKRGIEKAVERVVEQIGQMARDIETQEEIAHVATISANNDPE
                     IGTVIAEAMDKVGKDGVITVEESQTFGLELDFVEGMQFDKGYISPYMVTDTDRMEAVF
                     EEPFILIANQKISAVHDLLPILEKVMQAGRPLVIIAEDVEGEALATLVVNKIRGTFQS
                     AAVKAPGFGDRRKAMLQDIAILTGGQVISEEVGLKLDGVTLDLLGRARKVTIQKDNTT
                     IVEGAGEDADIKGRIQQIKNEIDNTDSDWDREKLQERLAKLSGGVAVIKVGAATEVEL
                     KEKKHRIEDALSATRAAVEEGIVAGGGTALLQASNALDKLELEGDYATGANIVRSALT
                     APLYWIAANAGLEGSVVVEKVRGMEAGQGLNALSGEYGDMIKFGVIDPAKVTRSALQN
                     AASIASLLLTTETVVADKPEPKEAGGAGGGHDHGMGGMDF"
     gene            1651..2568
                     /locus_tag="VNU01_10555"
     CDS             1651..2568
                     /locus_tag="VNU01_10555"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWH33097.1"
                     /translation="MAEWTDLFALAQRQYGLLERRQFGAAGVSVSAVDRRAVREAWQR
                     LHPGVLALPGSVDSPERRIMAAVLATGGEVAAARWTAAYLWGLTDRLRVPLTVVVAHG
                     RRAPRLDAVKTLRSRTLRPDDLTVRKGIAVTRPERMLADLAAVTTRPELRDLAIDVRQ
                     RRMLDVPVLWELYERTWPVRGHEHLKRVLLDLGGEQVDSGLEWRTRGLCRRAGLPEPF
                     PEPFPVQVEGRVVARIDIAWPDWKVGVECDGFRYHSERAQLERDTARQNLLVAHGWRL
                     CRVTWRQVEQESDTVIASIHRILQEAGAV"
     gene            2618..3397
                     /locus_tag="VNU01_10560"
     CDS             2618..3397
                     /locus_tag="VNU01_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33098.1"
                     /translation="MLSEAAVQGRLAEALGEAGGAWERGWVLAAMGRFGEALTVLEDA
                     MSGPERARALGTRASVYRQLSLHELAERDDDAGLAATDDPAVRAGLLVGKVADALGLG
                     LRGEWRPRALLAADSVAAAGDWRQRLRLAWVTGETAMYEGAPDAATPHFAEAVTRAVE
                     HGARRHETKSHLFLGAAHAALGNTAAAQGHARAALSLARDTGARPLQWAAALVLAEAG
                     ERPDEHVDEARRVLAAILDGLPDDLRAAALARDPARWLLGG"
     gene            complement(3394..4275)
                     /locus_tag="VNU01_10565"
     CDS             complement(3394..4275)
                     /locus_tag="VNU01_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWH33099.1"
                     /translation="MRIAVVGAGTMGAGIAYAAAVGGIEVALYDADPAGLERGLARAR
                     ADLDGAVARGKVEPAQADAAAARLRPARSLAAAVDRVDAVIEAIAEDMDAKQALFRQV
                     DALAPGALLATNTSALSVTQIMAVLEDPGRGLGMHFFNPVRAMRLCELVLGVQTRPDS
                     VERAEALARAMGKETVRVADVAGFATSRINAVIGNEAFFMLSEGVATAEDIDTAVRLG
                     LNHPMGPLEMADLVGLDVRLAVLGELRRSLGEKYRPAPLMEKLVRAGRLGRKTGQGVY
                     RYDAEGRREPGSAEGLR"
     gene            4327..>5020
                     /locus_tag="VNU01_10570"
     CDS             4327..>5020
                     /locus_tag="VNU01_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33100.1"
                     /translation="MRCVAVLLALAVALPACREEPVEPSLRARLAAPMVALAESVERV
                     EGMADSARYRVERGEPMREALALLRGSLDEVRAHAGELAHAAVGLRDPQAAAIARQTV
                     DLALQAADRADEEIAVLAPLVDADTLMDRLVATWAGAADDALVAQVAALPPALGAAPA
                     LPEPCRTLWDNRARWAALVADRSRRLAGTPEARDGFLANPFGEDRRAADSAERPCWRA
                     HGALPRALADLRP"
BASE COUNT          692 a         1764 c         1901 g          663 t
ORIGIN      
        1 ggtggccaag gagatcgagc tggacaaccc gttcgcgaac atgggcgccc agctcctcaa
       61 ggaggccgcg accaagacca acgacgtggc cggtgacggc accaccacgg cgacggtcct
      121 ggcgcaggcg atggtccgcg agggcatgcg caatgtcgcg gccggcgcca acccgatgct
      181 gctcaagcgc ggcatcgaga aggccgtcga gcgcgtggtc gagcagatcg gccagatggc
      241 gcgcgacatc gagacgcagg aggagatcgc gcacgtcgcg accatctccg ccaacaacga
      301 ccccgagatc ggcacggtca tcgccgaggc gatggacaag gtcggcaagg acggcgtgat
      361 caccgtcgag gagagccaga ccttcggcct ggagctcgac ttcgtcgagg gcatgcagtt
      421 cgacaagggc tacatctcgc cgtacatggt gaccgacacc gaccgcatgg aggcggtgtt
      481 cgaggagccc ttcatcctca tcgccaacca gaagatctcg gcggtgcacg acctgctccc
      541 catcctggag aaggtcatgc aggccggccg gccgctggtg atcatcgccg aggacgtcga
      601 gggcgaggcc ctggcgaccc tggtcgtgaa caagatccgc ggcacgttcc agtccgccgc
      661 ggtgaaggct cccggcttcg gtgaccgccg caaggccatg ctgcaggaca tcgcgatcct
      721 gaccggcggc caggtgatct ccgaggaggt cggcctgaag ctggacggcg tgacgctgga
      781 cctgctgggc cgcgcccgca aggtgacgat ccagaaggac aacaccacga tcgtcgaggg
      841 tgccggcgag gacgccgaca tcaagggccg catccagcag atcaagaacg agatcgacaa
      901 caccgactcc gactgggacc gcgagaagct ccaggagcgc ctcgccaagc tgtcgggcgg
      961 cgtcgcggtg atcaaggtcg gcgcggccac ggaggtcgag ctcaaggaga agaagcaccg
     1021 catcgaggac gccctgtcgg cgacccgcgc cgcggtggag gagggcatcg tcgccggtgg
     1081 cggcaccgcc ctgctccagg cttccaacgc gctggacaag ctggagctcg agggcgacta
     1141 cgccacgggc gccaacatcg tccgctcggc gctgaccgcg ccgctgtact ggatcgcggc
     1201 gaacgcgggc ctggagggct cggtcgtcgt ggagaaggtg cgcggcatgg aggcgggcca
     1261 gggcctcaac gccctgtccg gcgagtacgg cgacatgatc aagttcgggg tcatcgaccc
     1321 ggcgaaggtc acgcgctccg cgctccagaa cgcggcctcg atcgcgagct tgctgctcac
     1381 gaccgagacc gtcgtggccg acaagcccga gccgaaggag gctgggggcg ccggtggcgg
     1441 ccacgaccac ggcatgggcg gcatggactt ctaggagtcc ttcgtcccac agcacgcccg
     1501 gaaaggcccc gcttcggcgg ggcctttccg cgtccccccg cccctgcggg tgcactttgg
     1561 cgcgtatgtg cgtgcagatg cacccgcctc ggcccgggac ctgtggatga ccgagacggt
     1621 ggcggctcca tttgccgcag catgcgacgc atggcggaat ggacggatct ctttgcactg
     1681 gcccagcgcc agtacggcct tttggagcgg cggcagttcg gtgccgcggg ggtctcggtc
     1741 tcagcggtgg atcggcgggc ggtgcgcgag gcttggcagc gccttcatcc cggcgttctg
     1801 gcgttgccgg gaagcgtcga ttcgccggag cggcgcatca tggcggcggt gcttgccacg
     1861 ggcggcgaag tcgcggcggc gcggtggacc gcggcatacc tgtgggggct cactgatcga
     1921 ctccgggttc cgttgactgt tgtggtcgcc catgggcgac gggcgccgcg gctcgacgcg
     1981 gtgaagactc ttcgcagccg gacgctgcgc ccggacgatt tgacggtccg caaaggcatc
     2041 gccgtcaccc ggcctgagcg gatgctcgcg gatctcgcgg cggtgaccac ccggccggaa
     2101 ctgcgcgatc tggccatcga cgtgcgccag cgccggatgc tcgacgtccc tgtgctgtgg
     2161 gagctgtacg agcggacctg gccggtgagg ggtcacgagc acctgaagcg tgtgctgctc
     2221 gacctcggcg gcgagcaggt tgacagcggt cttgagtggc ggacgcgggg cctctgccga
     2281 cgggccgggc tcccggagcc ttttcctgag ccttttcccg tccaggtaga gggcagggtg
     2341 gtggcaagga tcgacattgc ctggccggat tggaaggtgg gcgtggaatg cgacggcttc
     2401 cggtaccact ccgagcgggc gcagctggag cgtgacacgg cccgccagaa tctcctggtg
     2461 gcccacggtt ggcggctgtg ccgggtgacg tggcgccaag tggagcagga gtcggacacg
     2521 gtcatcgcga gcatccacag aatcctgcaa gaggcgggtg cggtctgacg catatgcgcg
     2581 tcaaagtgca cccgcgttgg gcggtagggt gcgcgggatg ttgagtgagg cggcggtgca
     2641 ggggcggctg gccgaggcgc tgggcgaggc cggcggcgcc tgggagcgcg gctgggtgct
     2701 ggccgcgatg ggccggttcg gcgaggcgct caccgtgctg gaggacgcca tgtcgggtcc
     2761 tgagcgtgcc cgcgcgctcg gcacgcgcgc gtcggtgtac cgccagctct ccctgcacga
     2821 gctggccgag cgcgacgacg acgccggcct ggccgccacg gacgatcccg ctgtgcgcgc
     2881 cgggctgctg gtcggcaagg tggccgacgc cctggggttg gggctgcgcg gcgagtggcg
     2941 cccgcgtgcg ctgctcgccg ccgatagcgt ggcggccgcg ggggactggc ggcagcgcct
     3001 gcggctggca tgggtcaccg gtgagacggc catgtacgag ggcgcgccgg acgcggcgac
     3061 gccgcacttc gccgaggcgg tcacgcgcgc cgtggagcat ggcgcccgcc gccacgagac
     3121 caagtcgcac ctgttcctgg gggccgcgca cgccgctctg ggcaacacgg ccgccgcgca
     3181 ggggcacgcg cgcgcggcgc tgtccctggc gcgcgacacg ggcgccaggc cgctgcagtg
     3241 ggcggccgcg ctggtcctgg ccgaggcggg ggagcggccg gacgagcacg tggacgaggc
     3301 gcggcgggtc ctggcggcga tcctggacgg gctgccggac gatctgcgcg cggccgccct
     3361 tgcccgcgac cccgcccgct ggctgctcgg cggctagcgc agcccctcgg cggaaccggg
     3421 ctcgcgccgt ccctcggcgt cgtagcggta cacgccctgg ccggtcttgc ggcccagccg
     3481 ccccgcccgg accagcttct ccatcagcgg cgccggacgg tacttctcgc ccagggagcg
     3541 gcgcagctcg cccagcacgg cgaggcgcac gtccagcccc acgaggtcgg ccatctccag
     3601 cggccccatc gggtggttga gccccagccg cacggcggtg tcgatgtcct cggcggtggc
     3661 cacgccctcc gacagcatga agaacgcctc gttgccgatg accgcgttga tccgggaggt
     3721 ggcgaagccc gcgacgtcgg ccacgcgcac cgtctccttg cccatcgcgc gcgccagggc
     3781 ctccgcgcgc tccacgctgt ccggacgggt ctgcacgccc agcaccagct cgcacaggcg
     3841 catcgcgcgc accgggttga agaagtgcat gcccaggccg cggccggggt cctccagcac
     3901 ggccatgatc tgcgtcaccg acagcgcgga ggtgttggtg gcgagtagcg ccccgggcgc
     3961 cagcgcgtcg acctggcgga acagcgcctg cttcgcgtcc atgtcctcgg cgatcgcctc
     4021 gatcacggcg tcgacgcggt ccacggccgc ggccaggctg cgcgccgggc gcaggcgcgc
     4081 cgccgcggcg tccgcctgcg cgggctcgac cttgccgcgc gccaccgcgc catccagatc
     4141 ggcccgcgcg cgggccaggc cacgctccag cccggcgggg tcggcgtcgt acagcgcgac
     4201 ctcgatcccg ccgaccgcgg ccgcgtaggc gatgcccgct cccatcgtgc ccgcgccgac
     4261 gacggcgatg cgcatcggtg tctcctctcg tcggcgcgac agcctagccg cctaccatgg
     4321 ccgccgatgc gctgcgtcgc cgtgctgctc gccttggccg tggccctccc ggcgtgccgg
     4381 gaggagccgg tcgaaccgag cctgcgcgcg cgcctggccg cgcccatggt ggcgctggcg
     4441 gagtccgtgg agcgggtgga gggcatggcc gactccgcgc gctaccgggt cgagcggggc
     4501 gagccgatgc gcgaggcgct cgcgctcctg cgcggcagcc tggacgaggt gcgcgcccac
     4561 gccggcgagc tggcgcacgc ggcggtggga ctgcgcgacc cgcaggcggc cgccattgcc
     4621 cgccagaccg tcgacctggc gctccaggcc gccgaccgcg ccgacgagga gatcgccgtc
     4681 ctggcgccgc tggtggacgc cgacacgctc atggaccggc tcgtcgcgac gtgggcgggc
     4741 gcggcggacg acgccctcgt cgcgcaggtc gcggcgctgc ccccggcgct tggcgccgcg
     4801 cccgcgctgc cggagccgtg ccggacgctg tgggacaacc gtgcgcgctg ggccgccctg
     4861 gtggcggacc ggtcccggcg cctggccggc acacccgagg cccgcgacgg cttcctggcc
     4921 aacccgttcg gcgaggaccg gcgcgccgcc gacagcgccg agcgcccatg ctggcgtgcc
     4981 cacggggcgc tgccgcgggc cctggcggac ctgcgaccgc
//
LOCUS       DATMKC010000363         4310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_103332, whole genome shotgun sequence.
ACCESSION   DATMKC010000363 DATMKC010000000
VERSION     DATMKC010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103332"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="VNU01_10575"
     CDS             complement(<1..1627)
                     /locus_tag="VNU01_10575"
                     /inference="COORDINATES: protein motif:HMM:NF036452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5305 family protein"
                     /protein_id="HWH33101.1"
                     /translation="MTRAHGARRAVKAAAACGALLALAALWPAQLGGRTSFLSTVGGS
                     MAPQIREGDLAVVRSAPPYQVGDVVAYRNADLGQIALHRIIEARDGRFRLQGDANTWI
                     DSYEPAESEIVGRLWLHVPKAGTWLDWLRRPRDAALLTALAALLVGTGARHVRRRMRR
                     RRSGEPVRGAGLSRRGPAPAPSGAAGSRRLWLRAAPAGAALVAAGAIGSLVAGASPPL
                     RAQLEEVPYRHTGTFGYDAPATVSTDVLAGEAMRDGAPVYLRLAQRLNLRFAYALEAE
                     EVEGARGTRRLTAVLTDTRGWERELPLRPAATFDGPSFEVAGSLDLVGLQQLIQRFRD
                     QTGSGGTNFQLRVAVETAVEATVAGQGVRDEFEPDLVFLVDAHAMTPEPGEDGEPLTA
                     SRDGVVGRDVMVPARLEALGLSVPVSAMAAASRAVLAAGTLLLLVAGAALARSPAERP
                     SDRILRRHGSRILPVERVEPRPDERVVRMSEMRDLVRIADNLEAPVLHLAEGSLHRFL
                     VQEGATTYVVEVDDELGSQRDATVSRLPAQLGAP"
     gene            complement(1714..2121)
                     /locus_tag="VNU01_10580"
     CDS             complement(1714..2121)
                     /locus_tag="VNU01_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33102.1"
                     /translation="MKGLGLRRQVGVLLLPMVLATGAYGLTAANTVPDNDAGDGTVVV
                     TGYTVSAIDYNLNATNPYNIDSVDFLLTADDGSYELPDTIKIRLDASTTVWYSCAGQG
                     VATATDQTFLCDTTVGTQATVANTDELRVVAAE"
     gene            2357..2428
                     /locus_tag="VNU01_10585"
     tRNA            2357..2428
                     /locus_tag="VNU01_10585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2389..2391,aa:Val,seq:gac)
     gene            complement(2489..3337)
                     /locus_tag="VNU01_10590"
     CDS             complement(2489..3337)
                     /locus_tag="VNU01_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33103.1"
                     /translation="MSRRRVAVAAVVVGLTVAGGLSLPGSSAYLQGQSRTPGSVFGMG
                     RPPQEESLAETARRALCEAAAAEPCPAPQDFVPPWLREPGAAGADESVEPPPVAVTAA
                     PRAPATASPSPSPSPEPEEGCREPRCVDEAVLVVGEFADELRDLEQEEPGRVPERAVR
                     TIGEFADGLRELPEPPDDDLPLPEDTREALGELVEAGVLEPFPPQCVTNVGELAELVA
                     SRDGAADGEPSPSPEACATPSPSPSPSRRPEPTPNPSARPSPSPSPTPTEYPYDGPGH
                     RVRQAG"
     gene            complement(3334..3927)
                     /locus_tag="VNU01_10595"
     CDS             complement(3334..3927)
                     /locus_tag="VNU01_10595"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWH33104.1"
                     /translation="MSDIRQAQAAEESAPMQTGGAARTDGVLLTALRGLALVYVTLLV
                     LTGVAILGVGALLGWRPYLVTSGSMAPAMRPGDVIVVEPAVPGKPYDVPTIITFRDPA
                     RGLVTHRVVAAEQAPNGEVRYTTKGDANPDAESGTVGQSELVGSVRYVVRDVGMPLHW
                     AQHGRWGPFGAWAAGTLLAISLAVGLLRPRAAKESPS"
     gene            complement(3927..>4310)
                     /locus_tag="VNU01_10600"
     CDS             complement(3927..>4310)
                     /locus_tag="VNU01_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33105.1"
                     /translation="DLLPGQERVSRISVRNTGNVRLDVRLFGSVPVTAEHDLAAHLEF
                     EVHHVADGVAERVYPASGVATLRDFATDHAEYADGIDQWTASAGDLREYEFRVRLPEA
                     GDPSLPGDFGPGWSATVTFTWEGRS"
BASE COUNT          536 a         1649 c         1580 g          545 t
ORIGIN      
        1 ccggggcgcc gagctgcgcc ggcaggcggc tcaccgtcgc gtcgcgctgc gagcccagct
       61 cgtcgtcgac ttccaccacg taggtcgtgg cgccctcctg caccaggaag cggtgcaggc
      121 tgccctcggc caggtgcagg accggcgcct ccaggttgtc cgcgatgcgc acgaggtcgc
      181 gcatctcgct catgcgcacg acgcgctcgt cgggccgggg ctcgacgcgc tccaccggca
      241 ggatgcgaga gccgtgacgg cgcaggatgc ggtcggacgg ccgttcggcg ggcgagcgcg
      301 ccagcgcggc gcccgccacc agcagcagca gtgtgccggc ggccagcacg gcccgtgagg
      361 cggccgccat cgccgacacc ggcaccgaca gccccagcgc ttccaggcgg gcggggacca
      421 tgacgtcgcg gccgaccacg ccgtcgcgcg aggcggtcag cggctcgccg tcctcgcccg
      481 gctccggggt catcgcgtgg gcgtcgacca ggaacaccag gtccggctcg aactcgtcgc
      541 gcacgccctg gccggccacg gtggcctcca cggcggtctc gaccgccacg cgcagctgga
      601 agttcgtgcc gccggagccg gtctggtcgc ggaagcgctg gatcagctgc tgcaggccca
      661 ccaggtccag cgatccggcg acctcgaacg acgggccgtc gaacgtcgcc gccggccgca
      721 ggggcagctc ccgctcccag ccgcgcgtgt ccgtcagcac ggccgtgagc cggcgggtgc
      781 cgcgcgcgcc ctccacctcc tcggcctcca gcgcgtaggc gaagcgcagg ttcaggcgct
      841 gcgccagccg caggtagacg ggtgcgccgt cgcgcatggc ctcgccggcc aggacgtccg
      901 tgctcacggt ggcgggcgcg tcgtagccga acgtgccggt gtgccggtag ggcacctcct
      961 cgagctgggc ccgcagcggc ggactggcgc cggcgaccag ggagccgatc gctcccgcgg
     1021 ccacgagggc ggcgcccgcc ggtgcggcgc gcagccacag ccggcgtgat cccgccgcgc
     1081 cggagggcgc gggcgcggga ccgcggcggg acaggcccgc gccgcgaacc ggctcaccgc
     1141 tgcgccgccg cctcatgcgc ctcctcacgt gccgtgcgcc ggtgcccacg agcagcgccg
     1201 ccagagccgt cagcagggcg gcgtcgcgcg gccggcgcag ccaatccaac catgtgcccg
     1261 ccttgggcac gtgcagccac agccggccca cgatctcgga ctcggccggc tcgtagctgt
     1321 cgatccaggt gttcgcgtcg ccctggaggc ggaagcggcc gtcgcgggcc tcgatgatgc
     1381 ggtgcagggc gatctggccc aggtcggcgt tgcggtaggc gaccacgtcc cccacctggt
     1441 agggcggcgc gctgcggacg acggcgaggt cgccctcgcg gatctgcggc gccatggacc
     1501 cgcccacggt ggacaggaac gacgtgcgcc cgcccagctg cgccggccac agcgccgcca
     1561 gcgccagcag ggcgccgcag gctgcggctg ccttgaccgc cctgcgcgcc ccgtgtgctc
     1621 gtgtcatcgt catcccgccc gcccggacgt gacgcgaccg ccaggggcgt ggatgcccct
     1681 ggcggccggc gtcggctcat gtcgtggtgc gcactactcg gcggccacca cccgcagctc
     1741 gtcggtgttc gcgacggtcg cctgcgtgcc caccgtggtg tcgcacagga acgtctggtc
     1801 cgttgccgtc gccacgccct ggccggcgca cgagtaccag acggtcgtgg acgcatcgag
     1861 gcggatcttg atcgtgtcgg gcagctcgta gctgccgtcg tcggccgtca gcaggaagtc
     1921 caccgagtcg atgttgtacg ggttcgtggc gttgaggttg tagtcgatcg cgctcacggt
     1981 gtagcccgtg accacgaccg tgccgtcacc ggcgtcgttg tccggcacgg tgtttgcagc
     2041 cgtgagaccg taggcacccg tcgcgagcac catgggcaac agcaggacgc ccacctggcg
     2101 ccgcagtccc agacccttca tggatgtcac tccttgactc tcgcgagggc gatccgtcgc
     2161 ccgcttcccc gtaaagatcg ggagcggggg ctatggcctt gagtgactat gcgtgacccc
     2221 accgaaatgg tgatgcgcgc tacgtatgcc gtctacgtat gcctcacgcg gcatcagccg
     2281 cgcctcggcg gcgcgcctcg ccggcgcgcg gcgatgtgcc aagggggagg gcgccgacta
     2341 cacttggcct cctcccgggc ggttagctca gtgggagagc gctgccttga cacggcagag
     2401 gccactggtt caaacccagt accgcccacg cgccagcgca cacgagaggg cccgccgcac
     2461 ggcgggccct ctcggcgttg cgggacggtc atccggcctg gcgcacgcga tgccccggcc
     2521 cgtcgtaggg atactcggtc ggcgtggggc tcggcgacgg cgacgggcgc gcggacgggt
     2581 tcggcgtggg ctccggccgg cgtgagggcg acgggctcgg tgacggagtg gcgcaagcct
     2641 ccggcgacgg agacggctcg ccgtccgctg cgccgtcgcg gctcgccacg agctcggcca
     2701 gctctccgac gttggtgacg cactgcggtg ggaacggctc cagcacaccc gcctcgacca
     2761 gctctccgag cgcctcccgc gtgtcctccg gcagcggcag gtcgtcgtcc ggcggctccg
     2821 gcagctcgcg cagcccgtcg gcgaactccc cgatggtgcg cacggcgcgt tcggggaccc
     2881 ggccgggctc ctcctgctcc aggtcccgca gctcgtcggc gaactccccg acgaccagga
     2941 ccgcctcgtc cacgcagcgc ggctcccggc agccttcctc cggctcgggc gagggggacg
     3001 gcgacgggct cgccgtcgcg ggcgcccgcg gcgcggcggt cacggcgacc ggcgggggct
     3061 cgaccgactc gtcggcgccc gcggcgccgg gctcccgcag ccatggcggc acgaagtcct
     3121 ggggcgcggg acagggctcg gcggcggccg cctcacacag cgcgcggcgc gccgtctcgg
     3181 ccagggactc ctcctgcggg ggccggccca tgccgaacac gctgccgggc gtgcgcgact
     3241 ggccctgcag gtacgcgctg ctccccggca ggctgagccc tcccgccacg gtgaggccga
     3301 cgacgacggc ggccaccgcg acccggcggc ggctcacgac ggactctcct tggccgcgcg
     3361 gggccgcagc agccccaccg ccagggagat cgccagcagc gtgccggcgg cccaggcgcc
     3421 gaacgggccc cagcggccgt gctgcgccca gtgcagcggc atgcccacgt cgcgcaccac
     3481 gtagcgcacg gacccgacga gctccgactg gcccacggtg ccggactccg cgtccgggtt
     3541 cgcgtcgccc ttggtggtgt agcggacctc gccgttcggc gcctgctcgg ccgcgacgac
     3601 ccggtgcgtc accaggccgc gtgccgggtc gcggaacgtg atgatcgtcg gcacgtcgta
     3661 gggcttgccg ggcacggccg gctccaccac gatcacgtcg cccggccgca tggccggcgc
     3721 catggagccc gaggtcacca ggtacggccg ccagcccagc agcgcgccga cgccgaggat
     3781 cgccacgccc gtcagcacca gcagcgtcac gtacaccagc gccaggccgc gcagggccgt
     3841 gagcagcacg ccgtcggtgc gcgccgcgcc gccggtctgc atgggtgcgg attcctcggc
     3901 cgcctgtgcc tgccggatgt cgctcatcag ctccgccctt cccaggtgaa cgtgaccgtg
     3961 gcgctccagc ccgggccgaa gtcgcccggc aggctggggt cgcccgcctc cggcaggcgc
     4021 acgcggaact cgtactcgcg caggtcgccg gccgacgcgg tccactggtc gatgccgtcg
     4081 gcgtactcgg cgtggtccgt cgcgaagtcg cgcaaggtcg ccacacccga cgcgggatac
     4141 acgcgctcgg ccaccccgtc ggcgacgtgg tggacctcga actcgaggtg cgcggccagg
     4201 tcgtgctccg cggtcacggg caccgagccg aacaggcgca cgtccagccg cacgttgccc
     4261 gtgttgcgca ccgagatgcg gctgacgcgc tcctgcccgg gcagcaggtc
//
LOCUS       DATMKC010000364         9879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_68304, whole genome shotgun sequence.
ACCESSION   DATMKC010000364 DATMKC010000000
VERSION     DATMKC010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68304"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            574..2568
                     /locus_tag="VNU01_10605"
     CDS             574..2568
                     /locus_tag="VNU01_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33106.1"
                     /translation="MASARRPEGPFPYPSTSAWRVKFAQALLDDPRVNLPAEAIEELA
                     AGTLNHRELLVTSGGKTRFRPGLIAEHEALPGVTIYVLTATAHGAFGTVSPHNERIAT
                     WWPSPLDTSSPSDARLPERRRIDGHAAFVVDADSDIRLVDILSRTEAHLQSVDATRPY
                     DLTEDLATNGQRIPAMYVATRILTADNTRLAWTALTGNNRTLRRLELFGLRARDGVMG
                     APVRAVDGDPDATGVYTDTTAWLTRLSARLNREVASETDQDSPAQRAAAVARVHSEFV
                     VGCSDPDRLAEALHELNVREHVRGVHEYASLERHLAQGETVVVAYRREGLVDPVMADV
                     LVGAVAFDALRGHQPNPNWPVLTEADLADDAAARTWRHRALIELLLPTHPQHQRIVRR
                     AMGEPATSQLRKAHAEARGQTLAALQGLDQPKGRRNPRLGEHLTKRQAQSGISVSDRS
                     LDALVADLGASSEELVWRLTPILAGLGLVEPDRGSANARGGARQAIEAVLAAGSRGRT
                     FLSEALAAHREGRPPRRVDAGGAIIEVVADKEWVGTQDGFPRRPRPRPARPVTPPVEP
                     DPPKPAREAFAELAMQVPLLLAQLKQAPDELRRHLADMADLSSADASVGLSPDDADQV
                     KLELTNIGSAVHRLAGDVDPLVRATPDEDSEDGYDEEASA"
     gene            2565..2987
                     /locus_tag="VNU01_10610"
     CDS             2565..2987
                     /locus_tag="VNU01_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33107.1"
                     /translation="MTAHVHGRLPAPHLPPAAGLVLRAAAGRWYQETIGPVPVSQRHR
                     IQPWPVSVYLVTGRDGRIEWLGKADRDPGVAARLDDHLRHPDRRATFYQVRAVLLRPD
                     TPPAAVLALEGKLADVLSLRGWPGRAWPSAAMWPELVA"
     gene            complement(3024..4106)
                     /locus_tag="VNU01_10615"
     CDS             complement(3024..4106)
                     /locus_tag="VNU01_10615"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH33108.1"
                     /translation="MAFGHEVGRALSDGQVIGRSEPFAKVFPSSRVVKRAVGLVAWGV
                     LEDIALDAYLDGNGRLVAETSVRRIARNLGLNKDTVTRHLAKLREYGFVLHEENRDDG
                     NGRYATCRYVLDPSACIERFTTTPTVPNTAREARTGPPRGRGQVRGPRPAPQPACAEP
                     RRGTEPGELDLQLRAELERLGVAVPTAAQLVEQFPPQRIRAALDAVGKARPRSPAGWV
                     IAAIRQGWTLPTTGVASRPTDTSAAAGPATDTRSADLWDQWDLAISALLSDADLRALA
                     IVLCPPVPGVGWLIPAVRVEIIRWATYQCRDAVAVDADRIRTELAQPTAPPSDWEGPS
                     RPAGPATGQAVGLRARLPAVLLGRAR"
     gene            complement(4414..4959)
                     /locus_tag="VNU01_10620"
     CDS             complement(4414..4959)
                     /locus_tag="VNU01_10620"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HWH33109.1"
                     /translation="MAELTSLLRDDSLTRAQRLTRLQGWRGVDAACAEAAPAAQRIGP
                     AVCAVGALLGAALQAPLVPALFAATALAGAVAPHHPFESLSNRWARRRGSGALPPNRA
                     AKRFGCAVGVAFLGGAALAFASGATTLGVVLSLMLGCTAAFVAVTGICLPSMLFTVLW
                     GAERAAAPHLFGAPDSARSDA"
     gene            5055..5741
                     /locus_tag="VNU01_10625"
     CDS             5055..5741
                     /locus_tag="VNU01_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWH33110.1"
                     /translation="MGNRAAKDALFEAFAEVAKALASGRRAEIADVLAQGERSVEEIA
                     EELGQSLANTSHHLRAMARAGLLTTRRDGTRIYYALASERVGELWSALRDVAIEHVAG
                     IERLAGAYLGDRDGVEVVDRKELLARLKRREVVVLDVRPEAEFAAGHIAGARSMPIAE
                     LRRRMRALPKDAEVVAYCRGPYCVYADEAVRELTRKGFRAKRLKDGFPEWRRAGLPVA
                     AGTGGDTDER"
     gene            5731..6336
                     /locus_tag="VNU01_10630"
     CDS             5731..6336
                     /locus_tag="VNU01_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH33111.1"
                     /translation="MSDDLLVDPEALREQVRDKYREVATEPHRGFHFHTGRPLAARLG
                     YDDAAVDALPDRAVESFAGVGNPFSLRRLEPGERVVDVGSGAGFDSLIAAGQVGDSGH
                     VVGVDMTPEMLDKSRATAAALGLRNVEFREGLAERLPVEDGWADVVVSNGVINLCADK
                     KAVFEEIRRVLKPGGTLQFADIANGRPVPPDALRDIDLWTG"
     gene            6373..6504
                     /locus_tag="VNU01_10635"
     CDS             6373..6504
                     /locus_tag="VNU01_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33112.1"
                     /translation="MLEDVGFVDVVIGPAADTFGGAGGEDKARAYDVFGYAFLARRP"
     gene            6538..6903
                     /locus_tag="VNU01_10640"
     CDS             6538..6903
                     /locus_tag="VNU01_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWH33113.1"
                     /translation="MSNKAVISLTTGLEDAEKVTVAFLVAVGAAETGRPTLMFLTKEA
                     VRLALEGVAVGVACEGCPPLQDLMKRFEAAGGRYYVCPICFNAKQLDEASLISGAELQ
                     GTIPMWNWIGDEAATTFSY"
     gene            complement(6900..7151)
                     /locus_tag="VNU01_10645"
     CDS             complement(6900..7151)
                     /locus_tag="VNU01_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH33114.1"
                     /translation="MDKTLHSAEQGRLVELLRQMRVEARLTQAQVATRLGRPQSFVSK
                     YESGERRLDLVELAAVAEALETDLGGIVHRFQAAGPARG"
     gene            complement(8000..9118)
                     /locus_tag="VNU01_10650"
     CDS             complement(8000..9118)
                     /locus_tag="VNU01_10650"
                     /inference="COORDINATES: protein motif:HMM:NF038033.1"
                     /note="This lipoprotein, of unknown function, is related
                     to FEA1 (Fe-assimilating protein 1) from Chlamydomonas
                     reinhardtii (see PF07692); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FEA1-related lipoprotein"
                     /protein_id="HWH33115.1"
                     /translation="MRKTLRLLCAALALAVMTTACGNDDGAGVRVLDEDGSGSASGSG
                     SGSGSGSGSASGSASGVSSDPAGPLGGYEPASDVAAHAKVTLDVCAVNAALEQGDFEA
                     AAAAYAEGGNSLKGDGGARTLKGFASTPLDEPSWQAYAEHFGSDTWLDDFVTEALEGT
                     GRFAGASDEQRKQGVAKGVQNGVMVAWVLHELDAAKAKIDAGETDPAGGAPHNVDEGW
                     AFYHGENPDCAPYATADKRGADFGRGHAVNEGMLEAFGAARDAAIAGDAAAYAQAEAE
                     IRRLLTVTYVQAVEKYTAKAASGADDAAKQQAEGYAFYRVIAPRIAEASPEAAAALDE
                     ALGAEEPPTQEEADAALDALRAAYGELGVSAEDIGTLQ"
     gene            9316..9528
                     /locus_tag="VNU01_10655"
     CDS             9316..9528
                     /locus_tag="VNU01_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWH33116.1"
                     /translation="MLVCHCKVVTDRQIRAAIDAGARDEFDVAAACGAGSDCGGCIPT
                     VQQLLGECAGCPVAGVARSTLTLPAA"
     gene            9562..>9879
                     /gene="bfr"
                     /locus_tag="VNU01_10660"
     CDS             9562..>9879
                     /gene="bfr"
                     /locus_tag="VNU01_10660"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWH33117.1"
                     /translation="MQGNAQVIEFLNEALTAELTAINQYFIASKMADNWGLKALAKDY
                     YDESVAEMKHADILIERILLLDGTPNMQRLFNVQVGEDPIEQFRLNFEMEKAAVERYR
                     RGVQ"
BASE COUNT         1416 a         3539 c         3505 g         1419 t
ORIGIN      
        1 agtgacggtg cgcccaccgt cggcggtggg gattcggcag gagaagtacc aggtgactcg
       61 cggatgcccg cagccacaca gcttcagccc acgcgggaca cgcttgtggc agcgactgca
      121 ccgccggtag acatactcga ccactgggga gcacctttcg ccctgtcagg tggtccccgc
      181 aaagggtggc tacccctcgg cgctacgcga tatgcggccg ctccttctgg ccgcaccttt
      241 ggccagcgag ctgctgacag acgggtggtc ttcacctagt ggtcatcccg acacaggtgc
      301 ctggcaatga cgtgttcggg ctcatatcct gtttgctgag cgcgtctgct ggcagacgca
      361 ggatcggcac ttgccgcccg tgacgggtcg cacgctaggc caatgcaggg ctctcagccc
      421 tgcggccgag gtcctgtgtg caaaggcggc ggtcgaccac cccatgtcca cccgtgtgtg
      481 gatccgggca ttggccggcg gcaccgacct ctcgaccgtg aggggggagg cccactcgtc
      541 cccccaatcc agatcgagag caaaggttcc gtcatggctt ctgcccgacg gcccgaaggg
      601 ccgttcccgt acccgtccac ctctgcctgg cgtgtcaagt tcgcgcaggc gctgctcgat
      661 gatccgcgcg tgaacctgcc cgccgaggcg atcgaggaac tcgccgccgg cacgctgaac
      721 catcgcgagc ttctggtgac cagcggtggc aagaccaggt tccgcccggg gctgatcgcc
      781 gagcacgagg ccctgcctgg cgtgaccatc tacgtcctga ccgcgaccgc gcatggcgcg
      841 ttcggcaccg tgagcccgca caacgagcga atcgcgacgt ggtggccctc tccgctggac
      901 acgagctcgc cttcggatgc ccggctgccg gagcggcggc gtatcgacgg gcacgcggcg
      961 tttgtggtcg acgcggacag cgacatccgc ctagtcgaca tcctgtcgcg cacggaagcg
     1021 cacctgcagt cagtcgacgc cacgcgcccg tatgacctga cggaggatct ggccaccaac
     1081 ggccagcgca tcccggccat gtacgtggct acgcgcatcc tcaccgcgga caacacccgc
     1141 ctggcctgga ccgcgttgac gggcaacaac cggacattgc ggcggctgga gctgttcggc
     1201 ctgcgggcgc gggacggcgt catgggcgcg cccgtgcgtg ccgtggatgg cgatccggac
     1261 gccaccggcg tgtacaccga caccacggcc tggttgaccc gtctgtcggc gcgcctcaat
     1321 cgcgaggttg ccagcgagac cgaccaggac agtccagcgc agcgtgctgc cgctgtcgcc
     1381 cgcgtccact cggagttcgt cgtcggctgc tccgatcccg accggctcgc cgaggcgctc
     1441 catgagctga acgtgcgcga gcacgtccgg ggtgtccatg agtacgcctc gctggagcgc
     1501 cacctagccc aaggcgagac cgtggtggtg gcctaccgtc gggaaggttt ggtcgatcct
     1561 gtgatggccg acgtcctggt gggcgccgtc gccttcgatg cactccgcgg tcaccagccg
     1621 aacccgaact ggccggttct gaccgaggcg gacctcgctg atgatgccgc cgcccgcacc
     1681 tggcggcacc gggccctgat cgagctgctc ctgccgaccc accctcaaca ccagcgcatt
     1741 gtgcgccgag cgatgggcga gccggccacg tcgcagctac gcaaggctca tgccgaggcc
     1801 cgtgggcaaa ccctggcggc gttgcagggc ctggaccagc cgaagggccg tcgtaacccg
     1861 cggctcggtg agcacctcac caagcggcag gcccaatcgg ggatctccgt gagcgaccgg
     1921 tccctggacg cgctcgtcgc cgacctggga gccagcagcg aagagctcgt ctggcggctg
     1981 actccgattc tggctgggct cggcctggtg gaacccgatc gcggctcggc caacgcccgc
     2041 ggcggcgcgc ggcaggccat cgaagccgtc ctcgccgccg gtagccgtgg ccgcacgttc
     2101 ctgtcggagg cgctggcagc ccatcgcgaa gggcgcccgc cacgccgtgt cgacgccggt
     2161 ggcgcgatca tcgaggtggt ggccgacaag gagtgggtgg gaacccaaga tggtttcccg
     2221 cgccgcccgc ggccccgccc cgcccggcca gtgacgcctc ctgttgaacc cgacccgcca
     2281 aagccggccc gagaggcgtt cgccgagctc gccatgcagg tgccgctcct gctcgcccag
     2341 ctgaagcagg cgccggacga gttgcgtcgc cacctggccg acatggccga tttgtcttcg
     2401 gcagatgcca gtgtcgggtt gtcgccggat gacgccgacc aggtgaagct cgagctgacc
     2461 aacatcggca gcgctgtcca ccggctcgcc ggcgacgtcg acccgctagt gcgcgcgacc
     2521 cctgacgagg acagcgagga cggatacgac gaggaggcgt cggcgtgacc gcgcacgtgc
     2581 acggtaggct gcccgcgccg cacctccccc cggccgctgg gctcgtcctg cgcgccgcag
     2641 ctggccggtg gtatcaggag acgatcggcc ctgtcccggt ctcccaacgg catcgcattc
     2701 aaccgtggcc ggtaagtgtc tatctggtga cagggcgcga cggccgcatc gagtggctcg
     2761 gcaaggccga ccgagaccca ggcgttgcag ccagactgga cgaccacctg cggcacccag
     2821 accgccgcgc caccttctat caggtacgcg ccgtgctcct gcggccggac acgcctccag
     2881 cggccgtcct ggcgctagag ggcaagctcg ccgacgtcct gtccctgcgc ggctggccag
     2941 gccgggcatg gccatccgcc gccatgtggc ccgaactggt tgcctgaacg agcacgagcc
     3001 cgccaggcag agtcggggtt ggctcagcgc gcgcgaccca gtaggaccgc gggcagacga
     3061 gcccggagac cgacagcttg accggtcgcc gggccggctg gccgcgatgg gccctcccaa
     3121 tcgctgggag gtgcggtcgg ctgggccaac tctgtccgga tgcggtccgc gtctacggcg
     3181 accgcatcgc ggcactggta ggtggcccac cggatgatct cgactcgtac ggcggggatg
     3241 agccagccga cgcctgggac gggcgggcac agcacgatcg ccagggctcg gaggtcggcg
     3301 tcgctgagca gcgcggagat ggccagatcc cactggtccc acaagtccgc ggacctcgtg
     3361 tcggttgccg ggccggctgc ggccgacgtg tccgtcggcc gcgaggccac ccctgttgtg
     3421 ggcaacgtcc agccttggcg gatggcggcg atgacccagc cggcggggga gcggggccgg
     3481 gccttgccga ccgcgtcgag ggcggcgcgg atgcgctggg gtgggaactg ctcgacgagc
     3541 tgggcggcgg tgggcacggc gacgccgagc cgctcaagct cggcgcgcaa ctgcaggtcg
     3601 agctcgccag gctcggtgcc gcggcgaggc tcggcgcacg ccggctgcgg ggcgggccgc
     3661 ggcccgcgga cctgaccgcg accgcgaggc ggcccggtgc gcgcctcgcg ggcggtgttc
     3721 gggacggtcg gggtggtggt gaaccgttcg atgcacgcgg aggggtccag gacgtagcgg
     3781 caggtggcgt agcggccgtt gccgtcgtcg cggttctcct cgtgcagcac gaacccgtac
     3841 tcgcgcagct tcgccaggtg acgggtgacg gtgtccttgt tcagcccgag gttgcgggcg
     3901 atgcggcgca cgctggtttc ggcgaccagc cggccgttgc cgtcgaggta ggcgtccagg
     3961 gcgatgtcct ccagcacccc ccaggcgacc agtccgacgg ctcgcttcac cacccggctg
     4021 gacggaaaga ccttcgcgaa cggttcgctg cgcccgatga cctggccgtc ggatagggca
     4081 cggccgacct cgtgcccgaa cgccatcacg tcgcgggcct gccgcggtgc cgggccggtt
     4141 gtttgtccgg ttcgcgaagc ttcaatgtcg ctgctcaccg attggtcaca tggtggtcac
     4201 atggcgtcgt ggtaacccgt gccatccgtg ccactggcga cacagaaacg gcggagaatg
     4261 ccgctcctgg cgccagtggc acgaacgagg accggctcat aacccggagg tcgcaggttc
     4321 aaatcctgcc ctcgcaacca acgcgaaagc ccccgctccg gcgggggctt tcgcgttctc
     4381 cggcatccga gtcgctccac gaggcacccg cggtcacgcg tccgaccggg cgctgtctgg
     4441 cgcgccgaac aggtgcggcg cggcggcgcg ttcggcgccc cacagcacgg tgaacagcat
     4501 cgaggggagg cagatcccgg tgacggcgac gaacgcggcg gtgcagccga gcatgaggct
     4561 caggaccaca ccgagtgtcg tcgcgccgga ggcgaaggcc agcgccgcgc cgccgaggaa
     4621 cgccaccccg accgcgcagc cgaaccgctt cgccgcccgg ttggggggca acgcaccgct
     4681 gccacggcgg cgcgcccagc ggttggagag ggactcgaac ggatggtgcg gcgccaccgc
     4741 gccggccagg gctgtggcgg cgaacagtgc cggcacgagc ggcgcctgga gcgcggcgcc
     4801 cagcagcgcg ccgaccgcgc acaccgccgg gccgatgcgc tgggcggcgg gcgcggcctc
     4861 cgcgcacgcg gcgtcgacgc cccgccagcc ctggaggcgg gtcaggcgtt gcgccctcgt
     4921 cagggagtcg tcccggagga gggatgtcag ctcagccatg gacatgggcg cactgtcgcc
     4981 ccgtggtcgc catgttgtca agtaatcatt tgactacttg acaagaaggc cggcgcgggc
     5041 agaggatgcc cctcatggga aaccgggccg ccaaggacgc gctgttcgag gccttcgccg
     5101 aggtcgccaa ggcgctggcg agcggacggc gcgcggagat cgccgacgtg ctggcgcagg
     5161 gcgagcgttc ggtggaggag atcgccgagg agctcggcca gtcccttgcc aacacctcgc
     5221 atcacctgcg cgccatggcg cgcgccggcc tgctcaccac ccggcgcgac ggcacgcgca
     5281 tctactacgc cctggcgagc gaacgggtgg gggagctgtg gtcggcgctg cgcgacgtcg
     5341 cgatcgagca cgtcgcgggc atcgagcggc tggcgggcgc gtacctcggc gaccgtgacg
     5401 gcgtggaggt cgtcgaccgc aaggagctcc tggcccgact gaagcggcgc gaggtggtcg
     5461 tgctggacgt gcggccggag gcggagttcg ccgccggtca catcgcgggc gcgcgctcca
     5521 tgccgatcgc cgagctgcgc cgccgcatgc gggcgctgcc caaggacgcc gaggtggtcg
     5581 cctactgccg cggcccctac tgcgtgtacg cggacgaggc ggtgcgcgag ctgacccgca
     5641 agggcttccg cgcgaagcgc ctgaaggacg ggttccccga atggagacga gccggcctgc
     5701 ccgtggcggc cgggacggga ggcgacaccg atgagcgatg acctgctggt ggaccccgag
     5761 gcgctgcgcg agcaggtccg cgacaagtac cgggaggtgg ccaccgaacc ccaccgcggc
     5821 ttccacttcc acaccggtcg gccgctggcg gcgcgtctgg gctacgacga cgccgcggtc
     5881 gacgcgctgc cggaccgcgc cgtcgagtcc ttcgccggtg tcggcaaccc gttctcgctg
     5941 cgccgcctgg agccgggcga gcgcgtggtc gacgtcggct ccggcgccgg cttcgacagt
     6001 ctcatcgccg ccggccaagt gggcgacagc ggccacgtgg tgggcgtcga catgaccccg
     6061 gagatgctgg acaagtcgcg cgcgaccgcc gcggccctgg gcctgcgcaa cgtcgagttc
     6121 cgcgaggggc tcgcggagcg gctgcccgtc gaggacggct gggccgacgt ggtggtctcc
     6181 aacggcgtca tcaacctgtg cgccgacaag aaggccgtct tcgaggagat ccgccgggtg
     6241 ctcaagcccg gcggcacgct gcagttcgcc gacatcgcca acggccggcc cgtgccgccc
     6301 gacgccctgc gcgacatcga cttgtggact gggtgaattg ccggagggct gccccgtgcg
     6361 ggctggcaga agatgctcga ggacgtcggc ttcgtcgacg tcgtgatcgg accggccgcc
     6421 gacacgttcg gcggcgcagg cggtgaggac aaggccaggg cgtacgacgt cttcggctac
     6481 gccttcctcg cccgcagacc ctgatggcag cacccgacga cgacgaagga gaccaccatg
     6541 tccaacaagg cagtgatcag cctgaccacc gggctcgagg acgccgagaa ggtgacggtc
     6601 gccttcctgg tggccgtcgg cgccgccgag accggccgcc cgaccctgat gttcctcacc
     6661 aaggaggccg tccggctcgc ccttgagggc gtcgccgtgg gggtcgcctg cgagggctgc
     6721 ccgccgctgc aggacctcat gaagcgcttc gaggccgcgg gcggccgcta ctacgtctgc
     6781 cccatctgct tcaacgccaa gcagctggac gaggcgagcc tgatttccgg cgccgagctc
     6841 cagggcacca tccccatgtg gaactggatc ggcgacgagg cggcgaccac cttcagctac
     6901 tagcccctcg ccggacccgc ggcctggaag cggtggacga tgccgcccag gtcggtctcc
     6961 agcgcctctg ccaccgcggc cagttcgacc aggtccagcc ggcgctcgcc gctctcgtac
     7021 ttgctgacga aggactgcgg gcggccgagg cgcgtggcga cctgcgcctg ggtcagacgc
     7081 gcctcgactc gcatctggcg cagcagttcg accaggcgac cctgttccgc ggagtgcagc
     7141 gtcttgtcca cggcgccagt aggaatccca cctcgggata tcccaagttc ggttatgacc
     7201 ggctcgtcgg aatgaagtgc atccgtcaaa cactatcgaa tgccgcctcg cggatggcgc
     7261 ggtggagcgc tgcaccgacg gactttccaa cggtgtaccg atgccgggga tatcctggtt
     7321 cgggatattg accgcagtcg ctcgggccct taccgtttgg cctcgctcgg tgcggcggtc
     7381 ggcgaggctg cctgagtgct cggctggacg tgactgcgta gggcttgggg ggcggggaag
     7441 cggcagcggc cacccacgac tgacccaacc acgtacgcga gagcggccgc tcacggtgca
     7501 cccccatgca cctgtggcgg cttctcggat tcgcagcaag gcccttggca tccccccgcc
     7561 gagggccttg cgccgttcgg ggccgcggac gcagcggtcc gccaggcgcg acgccgtcgc
     7621 cgccgcgaag cgcagcgcca caccgagcca gcgcaatcgc tcctccggtg tggtccgcct
     7681 ccacgcctcg cgctgcgcgc gggcggcggc ctcccaggag ccgtctgcga cgctcattct
     7741 tggtcgctcc ggagtgccct gagtgccgcg atgtcggcga ggtcctgcgg gcggcccgct
     7801 tccgtcttca tggcgatgag gtgttccacg ctggccacgc gcacgggaac gtcctgcacg
     7861 tcgacgactt cgctcgcctc ccacagttcc gtgaacggtc cagcgccgcc ctgcgtcgat
     7921 gtccttgtgg gtgcatgggg cgggcgctga caagcgaagg cccccgggaa cccgggggcc
     7981 ttcgtggtcg gagagcgcgc tactggagcg tgccgatgtc ctcggcgctg acgcccagct
     8041 cgccgtaggc cgcgcgcagc gcgtcaagcg cggcgtcggc ctcctcctgc gtcggcggct
     8101 cctccgcgcc cagcgcctcg tccagggccg cggccgcctc cggggaggcc tccgcgatcc
     8161 gcggggcgat cacgcggtag aacgcgtagc cctcggcctg ctgcttcgcg gcgtcgtccg
     8221 cgccgctggc cgccttggcc gtgtacttct ccacggcctg cacgtaggtc accgtgagca
     8281 ggcgtcggat ctccgcctcg gcctgcgcgt acgcggcggc gtcaccggcg atggccgcgt
     8341 cgcgcgcggc gccgaacgcc tccagcatgc cctcgttcac cgcgtggccg cggccgaagt
     8401 ccgccccgcg cttgtcggcg gtcgcgtacg gcgcgcagtc cgggttctca ccgtggtaga
     8461 aggcccagcc ctcgtccacg ttgtgtggcg cgccgccggc cgggtccgtc tcgcccgcat
     8521 cgatcttggc cttcgccgcg tccagctcgt gcagcaccca ggcgaccatc acgccgttct
     8581 gcacgccctt ggccaccccc tgcttgcgct gctcgtcgga ggcgccggcg aagcggccgg
     8641 tgccctccag cgcctcggtg acgaagtcgt ccagccaggt gtcgctgccg aagtgctcgg
     8701 cgtaggcctg ccagctcggc tcgtccagcg gcgtggacgc gaagcccttc agggtgcgcg
     8761 cgccgccgtc gcccttgagc gagttgccgc cctcggcgta ggcggcggcc gcggcctcga
     8821 agtcgccctg ctccagggcg gcgttcacgg cgcagacgtc cagtgtcacc ttggcgtgtg
     8881 ccgccacgtc cgacgccggc tcgtagccgc ccagcgggcc ggccggatcc gacgagaccc
     8941 ccgaggccga gccggaggcc gaaccggacc cggacccgga acccgacccg gaaccggagg
     9001 ccgatcccga cccgtcctcg tcgagcacgc gcacgccggc gccgtcgtcg ttgccgcagg
     9061 cggtggtcat gaccgccagg gccagggccg cacagagcag ccgcagggtc ttccgcatga
     9121 ctgtcgagtc ctttcgccgc ctggcgccgc gcccgcgctg caccggcctt catgaagtta
     9181 ggtttcccta acgaacgcta ggcgcgcggc ggcccatcgt gcaaatccgc aggtcaggcc
     9241 cgtgtctggc gcgcgcgctc ccgggcacgt acgctttctc aagtaaggct gaccgaactc
     9301 gccggggagt gttcgttgct cgtctgccac tgcaaggtcg tcaccgaccg ccagatccgc
     9361 gccgccatcg acgccggcgc gcgcgacgag ttcgacgtcg ccgcggcctg cggcgccggc
     9421 agcgactgcg gcggctgcat ccccacggtc cagcagctcc tgggcgaatg cgccgggtgc
     9481 ccggtcgccg gcgtcgcccg gtccaccctg acactcccgg cggcgtaggc tcccggcgca
     9541 gccggacacc gaggagacag catgcagggc aacgcgcagg tcatcgagtt cctcaacgag
     9601 gcgctcacgg ccgagctcac cgcgatcaac cagtatttca tcgcgtcgaa gatggccgac
     9661 aactggggcc tgaaggcgct cgcgaaggac tactacgacg agtccgtcgc cgagatgaag
     9721 cacgcggaca tcctcatcga gcgcatcctg ctgctggacg gcacgccgaa catgcagcgc
     9781 ctgttcaacg tgcaggtcgg cgaggacccg atcgagcagt tccgcctcaa cttcgagatg
     9841 gagaaggccg ccgtggagcg ctaccgccgc ggcgtgcag
//
LOCUS       DATMKC010000365        15267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_80163, whole genome shotgun sequence.
ACCESSION   DATMKC010000365 DATMKC010000000
VERSION     DATMKC010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80163"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VNU01_10665"
     CDS             complement(<1..678)
                     /locus_tag="VNU01_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33118.1"
                     /translation="MAGAHLRRIPRGHLAAPFTAALGDARVGLVEAGPGYGKTTLLDD
                     IAAAWRHGASRWVAGQASADLRERLRRDGPPHLLLVDDADRLPPAQRAAVADLAAGLP
                     RAHRMVVVSAGACADVRRLADDLPGPRLSAADLLLGADDLARGLHGVVADPEAAARAL
                     LAASGGWAEGAGLALRAGVGAGGAAEEAAAHGAALVARRAEALPARRLAVLRRGLSLP
                     RLRDPLLR"
     gene            complement(969..2000)
                     /locus_tag="VNU01_10670"
     CDS             complement(969..2000)
                     /locus_tag="VNU01_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH33119.1"
                     /translation="MNRRRQGARLAAVAGTAAAGAAIAWAASRPDRGPLHVRDPEWAE
                     LHRDLGAQALSVASFDGTRLHADVLGPPGAPVIVLAHGYVLSARAWHYQVRDLAGEFR
                     VVAYDQRGHGRSQTAASGDYSIQALGRDLAAVLDAAAPDGVPAVVAGHSMGGMSVLSF
                     AEQFPDAVPARLAGAVLLSTTAADVVRGSVFSAGSALVATVLGAAAAKAVGLRPPQRV
                     HDHDVALFLTRLIGLAPSASPAHVAFTQQLALSCPKEVSASVLPALTALDVREAAGRL
                     GVPTLVMVGAADRITPPGAARELVSLLPSGELVVLPGVGHMAPLEAHRDVTAHLRAFA
                     RRCLERPAA"
     gene            complement(1997..3145)
                     /gene="alr"
                     /locus_tag="VNU01_10675"
     CDS             complement(1997..3145)
                     /gene="alr"
                     /locus_tag="VNU01_10675"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWH33120.1"
                     /translation="MTVSGARAEPPGQRPTWVEVDVDAIAHNVAALKAHAAAPRLMAV
                     VKADGYGHGLVPSARAALRGGADWLGVALVEEGAALRAAGIGAPVLVLAEPPASAVGE
                     LLRHRLTPAAYTRPFLEALDAAARGAGEPAGLHLKLDTGMRRVGVPPADWEDALAFVR
                     DAPGLRLDALWSHFAVADEPHHPFIGRQAAEFRRGLALADRLGVPPGFAHLCNSAGTL
                     HLHDEHHDMVRPGLAVYGLEPAPGLAGPVPLRPALSWYSRLSMVKPLAAGEAVSYGLR
                     WRAERDTVVGTVPAGYADGVVRALSDRGRVLVGGRTAPMRGTVCMDQFLVEVDGGAAA
                     GDEVCLIGRQGDEEVTADEWAGLLGTINYEIVCGIGSRVPRVHLGGQA"
     gene            complement(3142..4644)
                     /locus_tag="VNU01_10680"
     CDS             complement(3142..4644)
                     /locus_tag="VNU01_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWH33121.1"
                     /translation="MIPLYLPGSVRAMDERAIAGGTPSLELMERAAGHLARAVTAAAG
                     RSYGLRVAVLCGKGNNGGDGLAAARRLLDAGAAPLVWLAAAPEELSRDAAAQLARWRA
                     AGGRVAASAADALRDADVAVDCLLGTGAAGAPRGAVAGAIAAVTASGLPVVACDAPSG
                     VDASTGQVAGPAVRADVTVTLGAEKLGLRLWPARGLCGRLLHADIGVRNSGDAPDAVV
                     LEDADVAALLPAPEPDADKRSRGIVLIVAGSDGMAGAAVLAARGALASGAGLVTVMAP
                     EPVRPLIAQAVPAALTVALPHDPGHAADLVNERAADSDAAVFGPGRGRGDADVALARA
                     VVAGCAAPLVLDADGLNAYRHDGDALAGHASRLLVCTPHRRELERLTGEEDVWARRAA
                     AAPELAARWDTVLVAKGPGTVTAAPGGRRWVNATGSASLATGGTGDVLAGVLGALLAR
                     SPRPETVAAGVHLHGLAGEAAARERTVRGAGPVDVVRGLSEAWRRLEAAA"
     gene            complement(4641..5024)
                     /locus_tag="VNU01_10685"
     CDS             complement(4641..5024)
                     /locus_tag="VNU01_10685"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWH33122.1"
                     /translation="MAVIGIGVDAVEIARVDAALRRTPSLLARLFTERERAVCTSRCG
                     DLKAGGLAARFAAKEAVAKAFGTGLSGFAFRDIEVLSDPDGRPVVTLHGAAAERAAAL
                     GARVHVSLSTSKDLAVANAVLEQAP"
     gene            complement(5050..6900)
                     /gene="glmS"
                     /locus_tag="VNU01_10690"
     CDS             complement(5050..6900)
                     /gene="glmS"
                     /locus_tag="VNU01_10690"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWH33123.1"
                     /translation="MCGIVGYTGPEQALPIVMGGLRTLVYRGYDSAGVAVLHDGGVRV
                     HKRAGKLGVLEEALQDVELPGTTAIGHTRWATHGEPNDANAHPHLDAGGDIAVIHNGI
                     IENYAELKAELAAQDHQFLSDTDTEVVAHLIGSLRDGSDGDLADAVRTALKRVKGAFS
                     FAVVDRRQPHLIVAARREAPLVLGHVAGAALLSSDVAGLIAHTRDVEALLDDQVAVLT
                     PDGVQVTDLDGNPAEGHRYTVDWDVAAAEKQGYPHFMLKEIHEQPQAVRETLLGRIDE
                     GGRILLDAVRTDRDPQLELDLRSVDKVYVVACGTSYHAGMVAKYAIEHWAGIPVEMEV
                     ASEFRYRDPILTPHTLVVAISQSGETLDTLAAAAHARAQRAKVAAITNVVGSSLARDA
                     DLVLYTRAGPEIGVAATKTFVTQIVALDVLALYLAQQRRALFPEECRDHLARMEALGG
                     HIERVLEQEGAIAELAARFASADYVMFVGRHVGLPIALEGALKLKEISYVHAEGFPAG
                     EMKHGPIALIDQGGPVVALATAGHVHGKMLSNIQEVKARGAVVLAVATEGDTAIKEHA
                     DHVIFVPEVHELLYPVLTVVPLQLLGYHIASILGRDVDQPRNLAKTVTVE"
     gene            complement(7048..7926)
                     /locus_tag="VNU01_10695"
     CDS             complement(7048..7926)
                     /locus_tag="VNU01_10695"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HWH33124.1"
                     /translation="MADQQGADGLSKRERQKARGQQRRQQEMAKARREETTSRLLRLI
                     GLLVVLGLLGWFVGNWVVDWRRERQLISQAEERLEELGCDNGGIQPDQGAGHIEDPAA
                     VVPKAIYPDRPASSGPHFGSVVRSGVFDELVDERVLVHNLEHGYVNVYYAEDAPEEQV
                     AELKRIVGDKIGGSNPKMIVAPKIGEWSSDANFAFVAWRYRQMCEEFDEGVLLKFVSE
                     HYGLQGIAPEKTVPAHMGGQGVLDPDEDEGPLLLPPLTPEDLDPDAPAASPSPAPDAE
                     GTQEPAEATTEETAGG"
     gene            complement(7996..8994)
                     /gene="cofE"
                     /locus_tag="VNU01_10700"
     CDS             complement(7996..8994)
                     /gene="cofE"
                     /locus_tag="VNU01_10700"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWH33125.1"
                     /translation="MIQILPVTGLPEVGEGDDLAELVAGHASLADGDIVVVSQKIVSK
                     AEGALADVLPGETRADARRRLARSLAVRVLADTERALVVQTPQGLVCANAGIDASNVP
                     GGRLVLLPEDPDASARRLREGLRARTGADVAVIVADTFGRPWRLGQTDVAIGVAGIAP
                     LRDERGSVDREGVALEVTEIAVADQLAAAADLVRRKAEGVPVVIVRGALVERDEAGAA
                     RQLVRAPDEDLFPRGAGALTGPLGRLAESEPAWSAPVAADDVQGVLHVVRALGRAVPG
                     QEEEGPDGPTRFRADGVVLGVLAGLFADRGYEVRLRQSGQPGEAVLVAGRPAGQDG"
     gene            complement(8991..9932)
                     /gene="cofD"
                     /locus_tag="VNU01_10705"
     CDS             complement(8991..9932)
                     /gene="cofD"
                     /locus_tag="VNU01_10705"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HWH33126.1"
                     /translation="MDVVALAGGIGAARFLRGLVRVLDPSRLTVVANTGDDIVLHGLH
                     VSPDLDTITYTLGGGVHPEQGWGRAGESFVVSGELAERYGRPRWFSLGDRDLATHLVR
                     TQALRDGATLSQATARIAAAWGLPFALLPMTDDPVASRVRTADGRDLHFQEWWVGERA
                     KPDVADVRLDGAERARPAPGVLEAIAGADAVVLCPSNPVVSIGTILAVPGIREALRSA
                     VVVGVSPIVGGAVVRGMADRLLPAVGLEVSAAGVARGYADVLDGWVIDERDAALAAEV
                     ERLGVAVRVTDTIMRDVEVTAALASACLELARARTAT"
     gene            10094..11410
                     /locus_tag="VNU01_10710"
     CDS             10094..11410
                     /locus_tag="VNU01_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWH33127.1"
                     /translation="MRTEVVAGVTTFMTMAYILFVNPSILSATSDIPFPALLTATALA
                     AAVMTLAMGLVSNYPFAIASGMGLNAVVAFQLVGAQGLTYGEAMGVIVTEGVLITVLV
                     LTGFREAVLNAIPLALKKAIGAGIGLFITLIGFANAGIVVAGQGTPVARGNLDSAKVA
                     VFVVGLLVTAWLVARKTKGALLIGIVLTTVLAIVVNAANGGDLWQNGIAALPTDVVAA
                     PDFSLLGTFSLGYWGKLGAISAVLAVFTLMIADFFDTMGTAVALGNEGGFLDDDERLP
                     GMNRVLLVDSVAAAAGGLAGASSATTYIESAAGIEEGGRTGLTSVVVGLLFAVALFFS
                     PIAGVIPPEATAPALILVGFLMISVIRDIDWNDYAIAIPAFLTIVVMPFNYSITDGIG
                     WGFVSFTVIHLLLGRLRDVHWMMLLSSVAFAVFFAIDPIRRLLGVG"
     gene            complement(11432..12760)
                     /gene="glmM"
                     /locus_tag="VNU01_10715"
     CDS             complement(11432..12760)
                     /gene="glmM"
                     /locus_tag="VNU01_10715"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWH33128.1"
                     /translation="MAQLFGTDGIRGVANTELTPELAVGLGRALVRTLREEGIARPLV
                     AVGRDPRPSGEMLEAALVAGVTSAGGDIAPLGVLPTPGVAYLIGHVGADSGVMISASH
                     NPVEDNGIKVFGADGYKLSDAEEERVEELMQRTDEDRPTGLHIGRLRPRPDALGAYVD
                     HLAGSCGTDLGGMKVVVDCANGAAAGVAPAVLRRLGCDVVAINTRLDGDHINDGCGST
                     HPEVVAAAVREHKAEVGLSHDGDADRLIAADADGRLVDGDQILAVLADRLHRERGIDT
                     VVTTVMTNLGFRQAMAARGIDVVETKVGDRYVLEAMRSGGHLIGGEQSGHVILAEHAT
                     TGDGVLTAVQLLCAMRAAGKPLAELAGVMRRLPQVLVNVRGVDRSRLDGADELWAAVD
                     KESLDLADAGRILVRASGTEPLVRVMVEADTEERAAEVAERLAGIVRAAL"
     gene            complement(12775..13170)
                     /gene="rpsI"
                     /locus_tag="VNU01_10720"
     CDS             complement(12775..13170)
                     /gene="rpsI"
                     /locus_tag="VNU01_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWH33129.1"
                     /translation="MPAVITKIYTGRRKTSVARVRLVPGTGQFTLNGRPLEDYFQTEV
                     LQMTVREPFVTTETLGRYDVIARIHGGGTSGQAGALRHGISRALEGEDPEFRPLLKKA
                     GLLTRDAREVERKKYGLKKARKAPQYSKR"
     gene            complement(13184..13612)
                     /gene="rplM"
                     /locus_tag="VNU01_10725"
     CDS             complement(13184..13612)
                     /gene="rplM"
                     /locus_tag="VNU01_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWH33130.1"
                     /translation="MRTYSPRPADIQPQWYVIDAQDMVLGRLATRVAHVLRGKHKPTF
                     APHMDMGDFVIVVNADKVRLTGTKGDKELKYRHSGYPGGLKSIPFARLLAERPERAVE
                     QAVRGMLPKNTIGRAQLRKLKVYAGPEHPHTAQKPATLSA"
     gene            complement(14150..14527)
                     /locus_tag="VNU01_10730"
     CDS             complement(14150..14527)
                     /locus_tag="VNU01_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33131.1"
                     /translation="MLLLDAGALLALERDDRLTWRRLEMCVRDGVRAVTHAGVIGQVW
                     RGGQGRQARLAVALRGVEIVSLDDGLGRRAGVLLGRAGAADIVDAALVALAGDDDRIL
                     TSDAADIQALVDVFGRHVDVVPV"
     gene            complement(14527..14814)
                     /locus_tag="VNU01_10735"
     CDS             complement(14527..14814)
                     /locus_tag="VNU01_10735"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HWH33132.1"
                     /translation="MTKERVTVTIDAELAAAAAEAVREGRADSVSAWVNAALKERADK
                     ESKLAALRAAIADYEAEYGAFTDEELAAQERADREAAARVREEARRRRAAT"
     gene            complement(14846..>15267)
                     /locus_tag="VNU01_10740"
     CDS             complement(14846..>15267)
                     /locus_tag="VNU01_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33133.1"
                     /translation="RWAPPASMAPLWRHDTWHVGPPALDVDAMEAGGAHLLGEHDFSS
                     FCRRADDQHLVRRIDLLAVRREPGGLVVVRVEGKAFCHQMVRSVTACLVRVGQGKRPP
                     DWVREALHARDRHAVGSIAPPHGLTLVDVSFARPSAQ"
BASE COUNT         1938 a         5910 c         5456 g         1963 t
ORIGIN      
        1 ccgcagcagg gggtcgcgca ggcgcggcag ggacaggccg cggcgcagga cggcgagccg
       61 cctcgccggc agggcctcgg cacggcgcgc gacgagcgcg gcgccgtgcg cggccgcttc
      121 ctccgcggcg ccgcccgcac cgacccccgc gcgcagcgcc aggccggcgc cctcggccca
      181 gccgcccgag gcggccagca gcgccctggc ggcggcctcg ggatcggcga ccacgccgtg
      241 cagcccgcgc gccaggtcgt cggcgcccag cagcaggtcc gcggccgaca ggcgcggccc
      301 gggcaggtcg tcggccagcc ggcggacgtc cgcgcacgcg cccgcgctca ccacgaccat
      361 gcggtgtgcc cgcggcaggc cggccgcgag gtcggccacg gcggcccgct gcgcgggcgg
      421 caggcggtcc gcgtcgtcga ccagcagcag gtggggaggc ccgtcccggc gcagacgctc
      481 gcgcaggtcc gcggaggcct gcccggcaac ccagcgcgac gcaccgtgcc gccacgccgc
      541 ggcgatgtcg tccagcaggg tggtcttgcc atagcccggg cccgcctcca ccagcccgac
      601 ccgggcgtcg cccagcgcgg ccgtgaacgg cgccgccagg tgaccgcgcg gaattctccg
      661 cagatgcgcg ccggccatgc tccgtcgtcc cccctttcgc gcgtggcccc atgcgggagg
      721 catgggtcgc gcggcacagt aacgaggggc cgtaccagcg acgcatcgtc ggcgtaccag
      781 ttgcgtacca attgcgtccc agtggcgtcc cagcggcgta ccgaggtgtc gcgggcggcg
      841 ccgccggggg cgcgtgccgg aggtggcccc ctagtcctgg tgcgacagta ggcagggcct
      901 gtgacgagaa agcgccgcag gcgcctggtt gtccacaccg ggccgggcgg gacggggcac
      961 ggcgccgctc aggcggcggg ccgctccagg cagcgccgcg cgaacgcgcg caggtgcgcg
     1021 gtcacgtcgc ggtgcgcctc cagaggcgcc atgtgcccca cgccgggcag gacgaccagc
     1081 tcgccggagg gcagcaggct caccagttcc cgggccgcgc ccggcggtgt gatgcggtcc
     1141 gccgctccga ccatgaccag ggtgggcacg cccaggcgcc ccgccgcctc gcgcacgtcc
     1201 agtgcggtca gcgccgggag cacggacgcg ctcacctcct tggggcacga cagcgccagc
     1261 tgctgggtga acgccacgtg cgccggcgac gccgacggcg ccaggccgat cagccgcgtc
     1321 aggaacaggg cgacgtcgtg gtcgtgcacc cgctggggcg ggcgcaggcc gacggccttg
     1381 gcggcggccg cgcccaggac ggtggcgacc agcgccgacc cggcggagaa caccgagccg
     1441 cgcacgacgt cggcggccgt cgtgctcagc aggaccgcac cggccaggcg ggcgggcacc
     1501 gcgtccggaa actgctcggc gaaggacagc accgacatgc cgcccatgga atgcccggcc
     1561 acgacggccg gcacgccgtc gggcgccgcc gcgtccagga cggcggccag gtcacggccg
     1621 agcgcctgga tcgagtagtc gccggaggcc gccgtctggc tgcggccgtg gccgcgctgg
     1681 tcgtaggcca ccacgcggaa ctcgcccgcc aggtcgcgca cctggtagtg ccaggctcgt
     1741 gccgacagca cgtagccgtg ggccagcacg atgaccggag caccgggcgg ccccagcacg
     1801 tcggcgtgca ggcgcgtgcc gtcgaaggag gcgacgctca gcgcctgcgc gcccaggtcg
     1861 cgatgcagtt ccgcccattc gggatcgcgg acatggagcg gaccgcgatc cgggcgcgac
     1921 gccgcccacg cgatcgcggc gccggccgcc gccgtgccgg ccaccgcggc cagccgggca
     1981 ccctggcgcc gccggttcac gcctgcccgc ccaggtgcac gcgcggcacg cgcgagccga
     2041 tgccgcagac gatctcgtag ttgatggtgc cgagcaggcc cgcccattcg tcggccgtga
     2101 cctcctcgtc gccctggcgt ccgatgaggc agacctcgtc gccggccgcc gcgccgccat
     2161 ccacctccac caggaactgg tccatgcaca ccgtgccgcg catgggcgcc gtgcggcctc
     2221 ccaccagcac gcggccccgg tcggacaggg cccgcaccac gccatcggcg taaccggccg
     2281 ggacggtgcc gaccaccgtg tcgcgctcgg cccgccagcg cagcccgtag ctgaccgcct
     2341 caccggccgc cagcggcttg accatcgaca ggcgcgagta ccacgacagc gccgggcgca
     2401 gcggcacggg tcccgccagg ccgggcgccg gctccagccc gtagaccgcc aggccggggc
     2461 gaaccatgtc gtggtgctcg tcgtgcaggt gcagcgtgcc cgccgagttg cacaggtgcg
     2521 cgaagcccgg cgggacgccc agccggtcgg ccagcgccag gccgcggcgg aactcggccg
     2581 cctgccggcc gatgaacggg tggtgcggct cgtcggccac ggcgaagtgg ctccacagcg
     2641 cgtccagccg cagccccggc gcgtcgcgga cgaaggccag cgcgtcctcc cagtcggccg
     2701 gcgggacgcc gaccctgcgc atgccggtgt ccagcttcag gtgcaggccg gccggctcgc
     2761 cggcaccgcg ggcggccgcg tccagggcct ccaggaaggg gcgtgtgtac gccgccggcg
     2821 tcagccggtg acgcagcagc tcgccgaccg ccgaggccgg gggctcggcc agcaccagga
     2881 cgggcgcgcc gatccccgcc gcccgcaggg ccgcgccctc ctccaccagc gcgacgccca
     2941 gccagtcggc gccgccgcgc agggccgccc gcgccgacgg caccagcccg tgcccgtagc
     3001 cgtcggcctt cacgaccgcc atgagccgcg gcgccgccgc gtgcgccttg agcgccgcga
     3061 cgttgtgcgc gatcgcgtcc acgtcgacct cgacccacgt gggccgctgc ccgggcggct
     3121 ccgcccgcgc gccgctcacc gtcatgccgc cgcctccagt cgccgccatg cctcactcag
     3181 cccgcggacc acgtccacgg gccccgcgcc ccggaccgtg cgctcgcgcg cggccgcctc
     3241 gcccgccagc ccgtgcaggt gcacgcccgc cgcgacggtc tccggccgcg gcgagcgcgc
     3301 cagcagcgcg ccgagcacgc cggccagcac gtcgcccgtc ccgccggtgg ccagcgacgc
     3361 cgacccggtg gcgttcaccc agcgccgccc gccgggcgcc gcggtgacgg tacccgggcc
     3421 cttggccacc agcacggtgt cccagcgcgc cgccagttcg ggcgccgctg ccgcacggcg
     3481 ggcccacacg tcctcctcgc cggtgagccg ctccagctcg cggcggtgcg gcgtgcacac
     3541 caggagccgc gaggcatgac ccgccagggc gtcgccgtcg tggcggtagg cgttgagccc
     3601 gtcggcgtcc aggaccagcg gcgcggcgca gccggccacc accgcccggg ccagcgcgac
     3661 gtccgcgtcg ccgcggccgc gtcccggccc gaacaccgcg gcgtcgctgt cggccgcccg
     3721 ctcgttcacc aggtccgccg cgtggcctgg gtcgtgcggc agcgcgaccg tgagcgcagc
     3781 cggcaccgcc tgcgcgatca gcggcctgac cggctcgggg gccatgaccg tcaccagccc
     3841 cgccccgctc gccagtgccc cgcgggcggc cagcacggcg gcgccggcca tgccgtccga
     3901 gccggcgacg atcaggacga ttccgcggga gcgcttgtcc gcgtccggct ccggcgcggg
     3961 cagcagcgcc gcgacgtcgg cgtcctccag caccaccgcg tcgggggcgt ccccggagtt
     4021 gcgcacgccg atgtcggcgt gcagcagccg tccgcacagg ccgcgggcgg gccacagccg
     4081 caggccgagc ttctccgcgc ccagcgtgac cgtgacgtcg gcgcgcaccg cgggcccggc
     4141 gacctgcccg gtcgacgcgt cgacgccgga cggcgcgtcg caggcgacca cgggcaggcc
     4201 ggacgcggtc acggcggcga tggcgcccgc gaccgcgccg cgcggggcgc cggcggcgcc
     4261 cgtgccgagc aggcagtcca cggcgacgtc cgcgtcgcgc agggcgtccg cggcggacgc
     4321 ggccacgcgg ccccctgcgg cgcgccagcg cgcgagctgg gcggccgcgt cgcgcgacag
     4381 ctcctccggc gccgcggcca gccacaccag aggcgccgcg ccggcatcga ggagccggcg
     4441 ggcggcggcc agcccgtccc cgccgttgtt gcccttgccg cacaggacgg ccacgcgcag
     4501 cccgtaggac cgccccgccg ccgccgtgac cgcacgcgcc aggtggccgg ccgcgcgctc
     4561 catcagctcc aggctgggcg tgccgccggc gatggcgcgc tcgtccatcg cccgcacgct
     4621 ccccggcagg tacagcggga tcacggcgcc tgctccagca cggcgttggc cacggccagg
     4681 tccttgctcg tggacagcga cacgtgcacg cgcgccccca gtgcggccgc gcgctccgcc
     4741 gcggcgccgt gcagcgtcac cacgggacgg ccgtccgggt cgctcagcac ctcgatgtcg
     4801 cggaaggcga agccggacag gccggtgccg aaggccttgg ccaccgcctc cttcgccgcg
     4861 aagcgcgccg ccaggccgcc cgccttcagg tcgccgcagc gggacgtgca cacggcccgc
     4921 tcccgctccg tgaacagcct ggccaacagc gacggcgtcc ggcgcagggc agcgtccacc
     4981 cgcgcgatct ccaccgcgtc cacaccgatg ccgatcaccg ccatgccgtc gccgccgcgc
     5041 gccgcggcgt cactccaccg tgaccgtctt ggccaggttg cgcggctggt cgacgtcccg
     5101 gcccaggatc gaggcgatgt ggtagccgag cagctgcagc ggcaccaccg tgaggaccgg
     5161 gtacagcagc tcgtggacct cgggcacgaa gatgacgtgg tcggcgtgct ccttgatcgc
     5221 cgtgtcgccc tccgtcgcca ccgcgagcac gacggcgccg cgcgccttca cctcctggat
     5281 gttcgacagc atcttgccgt gcacgtgccc ggcggtggcc agggcgacca ccggcccgcc
     5341 ctggtcgatg agcgcgatgg gaccgtgctt catctcccct gcggggaagc cctccgcgtg
     5401 gacgtagctg atctccttga gcttgagcgc gccctccagc gcgatgggca ggcccacgtg
     5461 ccggccgacg aacatgacgt agtccgccga ggcgaagcgc gcggccaact ccgcgatggc
     5521 gccctcctgc tccagcaccc gctcgatgtg gccgcccagc gcctccatgc gcgccaggtg
     5581 gtcgcggcac tcctcgggga acagtgcgcg gcgctgctgc gccaggtaca gcgccagcac
     5641 gtccagcgcc acgatctggg tgacgaacgt cttggtcgcc gcgacgccga tctccggccc
     5701 cgcccgcgtg tacagcacca ggtccgcgtc ccgcgccagc gagctgccca cgacgttggt
     5761 gatcgccgcg accttcgcgc gctgcgcccg ggcgtgggcg gcggccgcca gcgtgtccag
     5821 cgtctcgccg gactggctga tcgccacgac cagggtgtgc ggcgtcagga tggggtcgcg
     5881 gtagcggaac tcgctggcga cctccatctc gacggggatc ccggcccagt gctcgatcgc
     5941 gtacttggcc accatgccgg cgtggtagct ggtgccgcag gccaccacgt agaccttgtc
     6001 caccgagcgc aggtccagct ccagctgcgg gtcgcggtcg gtgcgcaccg cgtccagcag
     6061 gatgcgcccg ccctcgtcga tgcgcccgag cagggtctcg cgcacggcct gcggctgctc
     6121 gtggatctcc ttgagcatga agtgcgggta gccctgcttc tcggccgccg ccacgtccca
     6181 gtccaccgtg tagcggtggc cctcggcggg gttgccatcc aggtccgtga cctgcacgcc
     6241 gtccggcgtc aggacggcga cctggtcgtc cagcagcgcc tcgacgtcac gggtgtgggc
     6301 gatcagcccc gccacgtcgc tggacagcag cgccgcgccc gccacgtgac cgagcacgag
     6361 cggcgcctcc cggcgcgccg ccacgatgag gtgcggctgg cgccggtcga ccacggcgaa
     6421 ggagaacgcc cccttgaccc gcttgagcgc ggtgcgcacg gcgtccgcga ggtcgccgtc
     6481 cgaaccgtcg cgcaacgagc cgatgaggtg ggcgacgacc tccgtgtccg tgtcggacag
     6541 gaactgatgg tcctgagcgg ccagctcggc cttgagctcg gcgtagttct cgatgatgcc
     6601 gttgtggatg acggcgatgt caccgccggc gtccaggtgc ggatgggcgt tggcgtcgtt
     6661 gggctcaccg tgcgtcgccc agcgggtgtg cccgatcgcc gtggtgccgg gcagctccac
     6721 gtcctggagc gcctcctcga gcacgccgag cttgccggcg cgcttgtgga cccgcacgcc
     6781 gccgtcgtgc agcacggcca ctcccgccga gtcgtagccg cggtacacca gcgtgcgcag
     6841 gccgcccatg acgatgggca gggcctgctc ggggccggtg tacccgacga ttccacacat
     6901 gtgctgcgcc tgcctgtcgc gatccgcggg acctgctcgg gtggtggccc aatcctaatg
     6961 gccggtacgc cgacggccgc gtgtcctggt ggacacgcgg ccgtcggcga acgggtgccg
     7021 cgccgggtcg gcgcgcggcg cagccggtca gccgccggcc gtctcctcag tggtggcctc
     7081 cgcaggctcc tgcgtcccct cggcgtcggg cgcgggcgac gggctggccg ccggcgcgtc
     7141 cgggtccagg tcctcgggcg tcagcggcgg cagcagcagc gggccctcgt cctcgtcggg
     7201 gtccaggacc ccctggccgc ccatgtgggc cggcacggtc ttctccgggg cgatgccctg
     7261 caggccgtag tgctcggaga cgaacttcag cagcacgccc tcgtcgaact cctcgcacat
     7321 ctggcggtag cgccaggcca cgaacgcgaa gttcgcgtca gaggaccatt cgccgatctt
     7381 cggcgccacg atcatcttgg ggttggaccc gccgatcttg tcgccgacga tgcgcttgag
     7441 ctcggcgacc tgctcctccg gcgcgtcctc ggcgtagtac acgttgacgt agccgtgctc
     7501 caggttgtgc accaggacgc gctcgtcgac cagctcgtcg aacacaccgc ttctcaccac
     7561 ggagccgaag tgcgggcccg acgaggccgg acgatccggg tagatcgcct tgggcaccac
     7621 ggccgccggg tcctcgatgt ggccggcgcc ctgatcgggc tggatgccgc cgttgtcaca
     7681 gcccagctcc tccaggcgct cctcggcctg cgagatgagc tggcgttcgc gccgccagtc
     7741 caccacccag ttcccgacga accagccgag cagtcccagg acgacgagca gcccgatcag
     7801 ccgcagcaag cggctggtgg tctcctcgcg ccgcgccttg gccatctcct gctggcggcg
     7861 ctgctgcccg cgtgccttct ggcgctcgcg cttggacagc ccgtccgcgc cctgctgatc
     7921 agccactggt gatttcctcc gtctcgaccg ggcgcccgcg gcgccgccgc gcaccccctc
     7981 acggcgtggg cgattctagc cgtcttggcc cgcgggccgt ccggccacca ggaccgcctc
     8041 gccgggctgg ccggactggc gcaggcgcac ctcgtagccg cggtcggcga acaggcccgc
     8101 cagcacgccc agcaccacgc cgtcggcgcg gaaccgcgtg gggccgtccg ggccctcctc
     8161 ctcctggccg ggcacggcgc ggcccagggc gcgcaccacg tggagcacgc cctgcacgtc
     8221 gtcggcggcc accggggcgc tccacgccgg ctcggactcg gccagcctgc cgagcgggcc
     8281 ggtgagcgcg ccggcgccgc gcgggaacag gtcctcgtcc ggcgcccgca cgagctggcg
     8341 cgcggcgccg gcctcgtccc gctccaccag cgcgccgcgc acgatgacca caggaacgcc
     8401 ctcggccttg cggcggacca ggtccgccgc cgcggcgagc tggtcggcca cggcgatctc
     8461 ggtgacctcc agcgccacgc cttcccggtc caccgagccg cgctcgtcgc gcaagggcgc
     8521 gatgcccgcg acgccgatcg ccacgtcggt ctggcccagg cgccacggcc ggccgaaggt
     8581 gtccgccacg atgacggcca catcggcgcc cgtgcgcgcg cgcaggccct cgcgcaggcg
     8641 gcgggcgctg gcgtccgggt cctcgggcag cagcaccagc cgcccgcccg gcacgttcga
     8701 ggcgtcgatg ccggcgttgg cgcacaccag cccctgcggg gtctgcacca ccagggcgcg
     8761 ttcggtgtcg gccagcacgc ggacggccag cgacctggcg aggcgccgcc gggcgtccgc
     8821 gcgcgtctct ccgggcagca cgtcggcgag tgccccctcc gccttgctga cgatcttctg
     8881 cgacaccacc acgatgtcgc cgtcggccag cgaggcgtgg ccggccacca gctcggcgag
     8941 atcgtcgccc tcgcccacct ccggcagtcc cgtgaccggc aggatctgga tcatgtcgcc
     9001 gtcctcgcgc gcgcgagctc gaggcaggcc gacgccagcg cggcggtcac ctcgacgtcg
     9061 cgcatgatcg tgtccgtgac gcgcaccgcg acgccgagcc gctccacctc ggcggccagg
     9121 gcggcgtcgc gctcgtcgat cacccagccg tccagcacgt cggcgtagcc ccgggcgacg
     9181 cccgccgccg agacctccag gcccaccgcg ggcagcaggc ggtcggccat gccgcgcacc
     9241 acggcgccgc ccacgatcgg cgagacgccg accaccacgg cggagcgcag cgcctcgcgg
     9301 atgccgggca ccgccaggat cgtcccgatc gagaccaccg ggttggaggg gcacagcacc
     9361 acggcgtccg cgccggcgat cgcctccagc acgccgggtg cggggcgtgc ccgctcggcg
     9421 ccgtcgagcc gcacgtcggc cacgtccggc ttcgcgcgct cgccgaccca ccactcctgg
     9481 aagtgcaggt cgcgcccgtc ggccgtgcgc acgcgcgagg cgaccgggtc gtcggtcatc
     9541 ggcagcagcg cgaagggcag cccccaggcc gcggcgatcc gcgccgtcgc ctgcgacagc
     9601 gtggcgccgt cgcgcagcgc ctgcgtgcgc accaggtggg tcgccaggtc acggtccccc
     9661 aggctgaacc agcgcggcct gccgtagcgc tccgccagct cgccggacac gacgaaggac
     9721 tccccggcgc ggccccagcc ctgttccggg tgcacaccgc cgccgagggt gtaggtgatc
     9781 gtgtccaggt cggggctcac gtgcaggccg tgcagcacga tgtcgtcgcc ggtgttggcg
     9841 acgaccgtga ggcgcgaggg gtccagcacg cggaccagcc cgcgcaggaa acgggcggcc
     9901 ccgatcccgc cggcgagcgc gacgacgtcc atgccgtgga ctgtagccgc cacagttgcg
     9961 cgggcgccgc cgcgagccgc cttatcctgc tgccggcatg gtcggtcggc gggggcatga
    10021 agggagcgca gcagtggccc agcaagcgtc cgttctggaa cgtgtcttca aggtgtccga
    10081 acgcggcacg agcgtgcgga ccgaggtggt cgcgggcgtc acgacgttca tgaccatggc
    10141 ctacatcctg ttcgtgaacc cgagcatcct gagcgcgacc tcggacatcc ccttccccgc
    10201 gctgctcacc gcgacggcgc tcgccgcggc cgtcatgacc cttgccatgg gcctggtcag
    10261 caactacccg ttcgcgatcg ccagcggcat ggggctcaac gcggtggtcg cgttccagct
    10321 cgtcggggcg cagggcctga cctacggcga ggccatgggc gtcatcgtga ccgagggcgt
    10381 cctcatcacg gtcctggtgc tgacgggctt ccgcgaagcc gtcctgaacg ccatcccgct
    10441 ggccctcaag aaggccatcg gcgcgggcat cggcctgttc atcaccctga tcggcttcgc
    10501 caacgccggc atcgtggttg ccggccaggg cacgccggtc gcgcgcggca acctggacag
    10561 cgccaaggtc gcggtcttcg tggtcggcct gctggtgacc gcgtggctgg tcgcgcgcaa
    10621 gaccaagggc gccctgctca tcggcatcgt gctcaccacg gtgctcgcga tcgtcgtcaa
    10681 cgcggccaac ggcggcgacc tgtggcagaa cggcatcgcc gccctgccca cggacgtggt
    10741 cgccgcgccg gacttctccc tgctgggcac cttcagcctg ggctactggg gcaagctggg
    10801 cgccatcagc gccgtgctcg cggtcttcac gctgatgatc gccgacttct tcgacaccat
    10861 gggcaccgcg gtggcgctgg gcaacgaggg cggcttcctc gacgacgacg agcgcctgcc
    10921 cggcatgaac cgcgtgctgc tggtcgactc cgtcgcggcg gcggcgggcg gcctggcggg
    10981 cgcctccagc gccacgacct acatcgagtc ggcggccggc atcgaggagg gcggccggac
    11041 ggggctgacc agcgtcgtgg tgggcctgct gttcgccgtg gcgctgttct tctcgccgat
    11101 cgccggcgtg atcccgcccg aggcgacggc gccggccctg atcctggtcg gcttcctgat
    11161 gatctcggtg atccgcgaca tcgactggaa cgactacgcg atcgcgatcc cggcgttcct
    11221 gaccatcgtg gtgatgccgt tcaactactc catcaccgac gggatcggct ggggcttcgt
    11281 cagcttcacg gtcatccacc tgctgctggg ccgcctgcgc gacgtgcact ggatgatgct
    11341 gctgtccagc gtcgccttcg cggtgttctt cgccatcgac ccgatccgcc gcctgctcgg
    11401 ggtgggctga gcgcgctccc ccgtgccccg ctcagagggc ggcgcggacg attccggcga
    11461 gtcgctcggc gacctcggcc gcgcgctcct ccgtatccgc ctcgaccatc acccgcacca
    11521 gcggctcggt tcccgaggcg cgcacgagga tgcgaccggc gtcggccaag tccaacgact
    11581 ccttgtccac cgccgcccac agctcatcgg cgccgtccag gcgcgagcgg tccaccccgc
    11641 gcacgttgac gaggacctgc ggcaggcggc gcatgacgcc ggccagctcc gccagtggct
    11701 tgccggcggc gcgcatcgcg cacagcagct gcaccgcggt gagcacaccg tcgcccgtcg
    11761 tggcgtgctc cgccaggatc acgtggccgg actgctcgcc gccgatgaga tggccgccgg
    11821 agcgcatggc ctccagcacg tagcggtcgc ccaccttggt ctcgaccacg tcgatgccgc
    11881 gcgcggccat cgcctggcgg aagccgagat tcgtcatgac ggtggtgacc acggtgtcga
    11941 tgccgcgctc gcggtgcagc cggtccgcga gcacggccag gatctggtcg ccgtcgacca
    12001 ggcgcccgtc ggcgtcggcg gcgatcaggc ggtcggcgtc gccgtcgtgt gacaggccca
    12061 cctcggcctt gtgctcccgc acggcggcgg ccaccacctc cgggtgggtc gacccgcagc
    12121 cgtcgttgat gtggtcgccg tcaaggcgcg tgttgatcgc cacgacgtcg cagcccaggc
    12181 ggcgcagcac cgccggcgcg acgcccgcgg ccgcgccgtt ggcgcagtcc acgaccacct
    12241 tcatgccgcc caggtcggtg ccgcaggagc cggccaggtg gtcgacgtac gcgccgagcg
    12301 cgtccggtcg cgggcggagg cggccgatgt gcaggcccgt ggggcggtcc tcgtccgtgc
    12361 gctgcatcag ctcctcgacg cgctcctctt ccgcgtcgct gagcttgtag ccgtcggcac
    12421 cgaacacctt gatgccgttg tcctcgacgg ggttgtggct ggccgagatc atcacgccgc
    12481 tgtcggcgcc cacatggccg atgaggtagg cgacaccggg ggtcggcagg acccccaggg
    12541 gcgcgatgtc gccgccggcc gaggtcacgc cggcgacgag cgccgcctcg agcatctcgc
    12601 cggaagggcg ggggtcgcgg cccacggcga ccagcggccg ggcgatgccc tcctcgcgca
    12661 gcgtccggac gagcgcacgc ccgagcccca cagcgagctc gggcgtgagt tcggtgttgg
    12721 cgaccccgcg gatgccgtcc gtgccgaaca gctgtgccac ggcggaaagg ggtcctagcg
    12781 cttggagtac tgcggcgcct tgcgcgcctt cttgaggccg tacttcttgc gctccacctc
    12841 gcgggcgtcg cgcgtgagca ggccggcctt cttcagcagc gggcggaact cggggtcctc
    12901 accctccagc gcgcgggaga tcccatggcg cagggctccc gcctgtcccg acgtgccgcc
    12961 gccgtggatg cgggcgatga cgtcgtaacg gcccagcgtc tcggtggtga cgaacggctc
    13021 gcgcaccgtc atctggagga cctccgtctg gaagtagtcc tccagcggac ggccgttcag
    13081 cgtgaactgg ccggtcccgg gaaccaggcg cacgcgtgcg accgaggtct tgcggcggcc
    13141 ggtgtagatc ttggtgatga cagcaggcat gtggtctgtt cctctacgcc gacagggtgg
    13201 cgggcttctg ggcggtgtgg gggtgctccg gccccgcgta caccttcagc ttgcgcagct
    13261 gcgcccgccc gatggtgttc ttggggagca tgccgcggac ggcctgctcg acggcccgct
    13321 cggggcgctc ggccagcagc cgcgcgaacg ggatcgactt gagcccgccc gggtagccgg
    13381 agtggcggta cttgagctcc ttgtcgccct tggtgcccgt gagccggacc ttgtccgcgt
    13441 tcaccacgat gacgaagtcg cccatgtcca tgtggggggc gaacgtcggc ttgtgcttgc
    13501 cgcgcagcac gtgggcgacg cgcgtggcca gccgcccgag gaccatgtcc tgggcgtcga
    13561 tgacgtacca ctgcggctgg atgtccgcgg ggcggggcga gtacgtgcgc atcaatggct
    13621 ctcagatcgg ttcggtggaa gtcggcgcgc gcgcgtgtcc gcgcggcgcg ccacggaagc
    13681 ccccagggta ggcgtgcgcc ggggtgcggt caacttcggc gcaggatcgg acccggcagg
    13741 tgtccacacc gcctatggat cgccgacgtg gatcccgcta cgctgtctcc accaggagca
    13801 gcccagcgat gggcgaatcc cgttagccga aggccccgct cgcgcggggt cttcgtgctt
    13861 acggagacca gcagtgaagg tgcaccacgt tggcctccac gtgggaccac aacatggcgt
    13921 tggcacgcag tgcacgggcg tcggtgacct gcccgctcat tctgcgtgca tgcagataga
    13981 ccaccagatc ggccgcctgc accaggcggc tggccaccga tggagcgaag tggagggtgt
    14041 ccacgatgcg agccagccgg cgggatcgat agccaccggc cgcgtgccgc tgaaagtgcc
    14101 agagaccttg gcggtatacg tcctgctggc ttacctgatc ggcgataaat cagaccggta
    14161 caacgtcgac gtggcggccg aagacgtcca ccagcgcctg gatgtcggcc gcgtcgctgg
    14221 tcaggattcg atcgtcgtcc ccggccaggg cgaccagggc ggcgtcgaca atgtcagcgg
    14281 cacccgcccg gccgagcaac acccctgccc gacgaccgag accgtcatcc agagagacga
    14341 tctcgacgcc gcgcagcgcc accgcaagtc gagcctgccg gccctggccg ccgcgccaca
    14401 cctgcccgat caccccggcg tgcgtcaccg cgcgcacacc gtccctgacg cacatctcca
    14461 accggcgcca ggtcagccgg tcgtcacgct ccagggccaa cagcgccccg gcgtccagca
    14521 agagcatcag gtggccgccc gccgccggcg ggcctcctcc cgcacccgcg cggccgcctc
    14581 ccggtccgcc cgctcctgcg ccgcaagctc ctcgtccgtg aaggccccgt actcggcctc
    14641 gtagtcggcg atggccgccc tgagcgcggc gagcttgctc tccttgtcgg cgcgttcctt
    14701 cagcgccgcg ttcacccagg cgctcaccga atcggcacgc ccttcccgca ccgcctcggc
    14761 cgccgcggcg gccaactccg cgtcgatggt cacggtcacc cgctccttgg tcataccttc
    14821 atcttacccc ggtatgacaa gcctcctact gcgcggacgg tctggcaaac gagacatcca
    14881 caagcgtgag gccgtggggc ggggcgatgg agcccaccgc atggcggtcg cgggcgtgga
    14941 gggcctcccg gacccagtcc ggcgggcgct tgccctggcc gacgcgcacc aagcacgccg
    15001 tgacggagcg caccatctgg tggcagaacg ccttgccctc gacgcgcacc acgacgagcc
    15061 cgccaggctc gcgccggacg gcgagcaggt cgatgcgccg gacgaggtgc tggtcgtccg
    15121 cccttcggca gaaggagctg aagtcgtgct cgcccagcag atgggctccc ccggcttcca
    15181 tggcgtccac gtccagcgcg ggcggtccca cgtgccaggt gtcgtggcgc cacagcggcg
    15241 ccatggaagc cgggggagcc catctgc
//
LOCUS       DATMKC010000366         9860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_286510, whole genome shotgun sequence.
ACCESSION   DATMKC010000366 DATMKC010000000
VERSION     DATMKC010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286510"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VNU01_10745"
     CDS             complement(<1..493)
                     /locus_tag="VNU01_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33134.1"
                     /translation="MRRLLSRTVALLGAVFAALLVVTPLMIATAQTPQTAEDVLMQDE
                     AWFFRYRQPLAPVPDVEDPAGASGIPKAEVRSRTSTPFAPETLHVAVVSGEQEAITFL
                     NLPLFELADNGLPPAVTGGTITLVDAGSSLGSKRADVAEMVACLVTELFVAALRGDEQ
                     LGHQ"
     gene            complement(705..1934)
                     /gene="tyrS"
                     /locus_tag="VNU01_10750"
     CDS             complement(705..1934)
                     /gene="tyrS"
                     /locus_tag="VNU01_10750"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWH33135.1"
                     /translation="MTSQHTAAEQLRVLLDGAEQVLPAEEFARKVTEAATGKRPPLRV
                     KFGMDPTSPDLHIGHTVVLRKLRQFQDLGHTAVLIVGGFTARVGDPSGRSVTRPALTA
                     QQVADNARTYLDQVGKVLLPERLEVVDNTDWLEPMGMADVLRLTAQATVAQMLERDDF
                     AKRYREGKPIAVSELLYPLLQGQDSVHLRSDVELGGTDQTFNLLVGRQLQSAAGQDPQ
                     CVLTMPLLEGLDGSAKMSKTSGNTIGIVEDPAEMFGKAMSIRDELMVKYFRLATDLAP
                     DEVDAIAAGLADGSLHPGQTKRRLAREIVRMYHSAQAAEAAEARFDVQFKERGIPEDI
                     PEFRVDTQPVWPLPSLLADAGLVPSRSEARRMVGQGAVRLDGEPLRDADAELRADELA
                     GRVLQVGKRRFVRLVRG"
     gene            2001..4427
                     /locus_tag="VNU01_10755"
     CDS             2001..4427
                     /locus_tag="VNU01_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWH33136.1"
                     /translation="MRRRVVLWVLAAVTLLALAGGAAAWRAAHAPVTLPPPAASSQIL
                     AADGSVLATLHGEQDRVPVPLERVPKVLRDAVIAAEDARFADHGGVDLRATLRALWRN
                     IRAGEATQGGSTITQQLAKNLVTGAEPTLGRKLREAWTAVRIEASLSKDEILERYLNT
                     VYFGGGAYGVQAASQRWFAKDVDDLELPDAALLAGLLRAPSGADPVRHPERARRARAR
                     VLDRMVAEGLVGRAEAEAVRRADLGVVETPPDWRAPWFVDHVLDTLRDDDRFAALGQD
                     ADARLDRLFRQGLRVETTLDPVWQAAAERAVREVVHEPGDPYAAVVAVDPRDGALRAL
                     VGGRRYGDPGDPVSRFNLATRGRRQAGSAFKPLVLAAALSRGHTLDDRFPAGRSVSIA
                     LPGDVAPWVVRNYDERAYGELALRDATRLSVNTVYARLVMDVGAEAVAELARVAGVGR
                     PLRPYPSIALGTQEVTPLEMAAVQATFAAGGVRRPVVAVRRVTDAGGAVLYEAEPARG
                     RRVLPEGVAALVTEALQDVVRRGTGQAAAIGRPVAGKTGTTQNWADAWFAGYTPRLAA
                     AVWVGFPQAAEPMTPPRTRERVEGGGWPAEIFAAFADAALSGVPAGEFAAPPGGVVRV
                     EVDRTRNCLPNAYTPQELVELRSYVPGSEPTEHCTEPSGPYDARVPDVVGLPFEAAAR
                     LLGGEGLLVARRAELRADLPPGRVVRQSPDADSPLSPDPALRPPVTVWVSAGAEVTVP
                     DVLGLELERAVAELEGRGLLVQVRQECPGAGRDCSGARLRPGTVWEQSPDAGRRVHFG
                     AQVTLFAYPA"
     gene            complement(4415..5005)
                     /locus_tag="VNU01_10760"
     CDS             complement(4415..5005)
                     /locus_tag="VNU01_10760"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWH33137.1"
                     /translation="MTERLERAFFARPATAVAPELVGKLLVREDQGLVARIVEVEAYL
                     QDDPACHAYRGQTARNAPLFGPPGHAYVYFTYGMHWCLNTVTGTPGHGQGVLLRAAEP
                     LENTELMRARRGGARDRDLLRGPARLAQAYGLDGSWSGADLCSGGVLYLADDGARPQV
                     AATPRTGVAAGFDTPWRFVVPGSPWASPYKRHPQAG"
     gene            complement(5002..6744)
                     /gene="polX"
                     /locus_tag="VNU01_10765"
     CDS             complement(5002..6744)
                     /gene="polX"
                     /locus_tag="VNU01_10765"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HWH33138.1"
                     /translation="MPWLNDEVARRFSEIATLLKLDGADPFRVRAYERAAGAISACPV
                     DLAELSDAEIAKLKGIGAATARKIAEFRDTGAIGMLDELKAQVPPGFTELVRVPGLGP
                     KTIRALHEALGIDSVEALRAALERDELKGIPGLGPKSVQNLRESLERAGAKQDKRIPV
                     EEALAVADELCARLAPLPGVHRVETAGSLRRMRETIGDVDILVSADGPAPVMAALRDS
                     DLVVKVIAAGERKTSVLTVRGLQVDLRVVAREEFGAALAYFTGSKEHNVRVRERAVRR
                     GLTLNEYGLFAKESGELVAGATEEDVYAALGLQWVPPTLREDTGEVDAAAEGRLPPVV
                     RLEDVRGDLHGHSDWSGDGKATLEELLEAAAAKGYAYYALTDHAENLTMNGLSRERML
                     AQREEVARLQERFPGMRILHGAELNIGIDGSLDYDEDFLLGYDFTVASVHSHFPRPRE
                     EQTARIVRAMEHPAVNVIGHPTGRKIGRRPGYEIDLEAVVEAAVRTGTALEVNAHPAR
                     LDLSGDMVRRAVEGGATLAISCDAHRVGDLDLMRYGVATAQRGWATPAQILNCRDLDG
                     LLAFVRAKRERSGA"
     gene            6800..7102
                     /locus_tag="VNU01_10770"
     CDS             6800..7102
                     /locus_tag="VNU01_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33139.1"
                     /translation="MPGPDSDLGHDLVAHLYAAALRLMHEHQQAFLPLNKVQEQLGWD
                     DESLGRVVDHCVRERPAITLPGAPSALTADWMDDGDRPAYGRSGVRFIGIRPAAPG"
     gene            complement(7071..8399)
                     /locus_tag="VNU01_10775"
     CDS             complement(7071..8399)
                     /locus_tag="VNU01_10775"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH33140.1"
                     /translation="MDQERGPGGMRQRSLPAILRPLRHAAYRRLAAGSLVSLFGDGVF
                     LVALPLQVYAISNIPTAMAVVGAVWTTSQLLMLLVGGWAVDRFERRRIMIAADLLRGA
                     ALLGAGALSLNGSLELWHFGVVGAVVGASNAFFNPAMTSIVPDLLPTGELARANAFLG
                     VARPAMVRLLGPAAGGLLVGTFGTGPAFLVDAGTFLVSAALLSRLPRRTADIDADAAA
                     SPPGSLRTVAEGLRFVRSHRWCWVWLIASAAGLLAFTGPVDMLLPYVVKNDLGLTQRQ
                     AAYFLGAVLAAGGGGSVLMALLVGQRGIPRRAMTAMYLAQALGIALLAMYGAMRSLWQ
                     GVLAAVLLNAMFTFTDIVWTTTLQRQVPRRLLGRVSSLDWMAALGAMPLSFVVAGRLA
                     EVSGAREVLLAASGVGAGVVLVLMMVPGARSPEGLEPAAALTPALRDESR"
     gene            complement(8541..>9860)
                     /gene="argH"
                     /locus_tag="VNU01_10780"
     CDS             complement(8541..>9860)
                     /gene="argH"
                     /locus_tag="VNU01_10780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWH33141.1"
                     /translation="LGRSVHFDARLWPYDITGSRAHADELRRLELLTAEDHAAIMGAL
                     DVVAGEFEASTFAFLDDDEDLHGAVERRLVELCGEAGGRLRAGRSRNDQICNDLRLYL
                     RDAIAELLDGVRSVQRALADKAEEVLDWIAPGYTHLQRAQPVALGHHLLAHAWALQRD
                     AGRLTDAARRMDVSVLGAGALAGLTLGLDPARYADALGFDRPAENSMDAVADRDFAVE
                     FLAAAAMAGVHLSRLGEEIVLWSSTEFGWARVGDAFSTGSSIMPQKRNPDVAELVRGK
                     TGRLIGDLVSLLVTLKGLPLTYNRDLQEDKEPVFDAVDTLRVALPAMAGTVRSLAFDK
                     DRLRAAAHGGFALATDLAEALVQRGVPFRQAHERVGAFVSECERRGLELDEVKRKNLV
                     TVFPELQDAPLDLLRPDAAVVRRGASLGPAPEPLRAQLAGLRALLDG"
BASE COUNT         1254 a         3687 c         3668 g         1251 t
ORIGIN      
        1 cctggtgacc gagctgttcg tcgccgcgca gggcggcgac gaacagctcg gtcaccaggc
       61 acgcgaccat ctcggccacg tcggcgcgct tggagcccag ggatgagccc gcgtcgacga
      121 gcgtgatggt cccgcccgtg acggccggcg gcagcccgtt gtcggccagc tcgaacaggg
      181 gcaggttcag gaacgtgatc gcctcctgct cgccggatac caccgccacg tgcagcgtct
      241 cgggggcgaa cggcgtcgac gtgcggctgc gcacctcggc cttggggatg ccgctggcgc
      301 cggccgggtc ctcgacgtcg ggcacggggg ccaaaggctg gcggtagcgg aagaaccagg
      361 cctcgtcctg catgaggacg tcttcagccg tctggggcgt ctgggcggtg gcgatcatca
      421 acggcgtgac gaccagcaga gccgcgaaga cagcacccag cagggcgacg gttcggctga
      481 gcagacggcg catggtgttc atcctcgggt ctggccaccg gtggcacacc ggcggcgtcg
      541 tctggacagt cctaccctgt ccaacgaggc aggtactatg aaggattcgc tagtccctac
      601 tacggtacca ggggccttcg cgcttacgga acgattcgcc cccgcgcgtt ccacgtccca
      661 atctaccgga cattccgggt gcttgcgtgg gagtcggccc gggctcaccc gcgcaccagg
      721 cgcacgaacc ggcgcttgcc cacctggagc acccggccgg ccagctcgtc ggcccgcaac
      781 tcggcgtcgg cgtcgcgcaa cggctcgccg tccagccgca cggcgccctg gccgaccatt
      841 cgccgggcct ccgagcggct gggcaccagg cccgcgtcgg cgagcaggct cggcaggggc
      901 cacaccggct gggtatccac ccggaactcc gggatgtcct ccgggatgcc gcgctccttg
      961 aactgcacgt cgaagcgcgc ctccgccgcc tcggccgcct gggccgagtg gtacatgcgc
     1021 acgatctcgc gcgccaggcg ccgcttggtc tgcccggggt gcagcgagcc gtcggcgagg
     1081 cccgcggcga tggcgtccac ctcgtcgggg gcgaggtcgg tggccagccg gaagtacttc
     1141 accatgagct cgtcgcggat cgacatcgcc ttgccgaaca tctcggccgg gtcctcgacg
     1201 atgccgatcg tgttgccgga ggtcttggac atcttcgcgg agccgtccag gccctccagc
     1261 aggggcatcg tcaggacgca ctgcgggtcc tggcccgcgg cggactgcag ctggcgtccc
     1321 accagcaggt tgaaggtctg gtccgtgccg cccagctcca cgtcgctgcg caggtgcacc
     1381 gagtcctggc cctgcagcag cgggtacagc agctcgctca cggcgatcgg cttgccctcg
     1441 cggtagcgct tggcgaagtc gtcgcgctcc agcatctgcg ccacggtggc ctgcgcggtg
     1501 agccgcagca cgtccgccat gcccatcggc tccagccagt cggtgttgtc gaccacctcc
     1561 aggcgctcgg gcagcagcac cttgcccacc tggtccaggt aggtgcgcgc gttgtcggcg
     1621 acctgctggg ccgtgagcgc cggtcgcgtg accgagcggc cgctggggtc gcccacgcgc
     1681 gccgtgaagc cgccgacgat gagcacggcg gtgtgcccga ggtcctggaa ctggcgcagc
     1741 ttgcgcagca ccacggtgtg gccgatgtgg aggtccgggc tggtggggtc catgccgaac
     1801 ttgacccgca gcggcggccg cttgccggtg gcggcctcgg tgaccttgcg cgcgaactcc
     1861 tcggcgggca ggacctgctc cgcgccgtcc aacaggacgc gcagctgctc ggcggcggtg
     1921 tgctggctcg tcatgacgcg caagcgtacc tatgcgccgc cgcacgccca tgccggcccg
     1981 gcgcccgtag catcgggggc atgcgccgtc gcgtcgtgct ctgggtcctg gccgcggtga
     2041 cgctgctggc cctggcgggc ggcgcggccg cctggcgggc ggcgcacgcc cccgtgacgc
     2101 tgccgccgcc ggccgcctcc agccagatcc tggccgccga cggctcggtg ctggcgacgc
     2161 tgcacggcga gcaggaccgc gtgcccgtgc cgctggagcg ggtgccgaag gtgctgcgcg
     2221 acgcggtgat cgccgccgag gacgcgcgct tcgccgacca cggcggcgtg gatctgcgcg
     2281 cgacgctgcg ggcgctgtgg cgcaacatcc gcgccggcga ggcgacgcag ggcggatcga
     2341 cgatcaccca gcagctcgcc aagaacctgg tgaccggcgc ggagcccacc ctgggccgca
     2401 agctgcgcga ggcgtggacg gcggtgcgca tcgaggcgtc gctgtccaag gacgagatcc
     2461 tggagcgcta cctcaacacc gtgtatttcg gcggcggcgc ctacggggtg caggccgctt
     2521 cccagcgctg gttcgccaag gacgtcgacg acctggagct gcccgacgcg gcgctgctgg
     2581 cggggctgct gcgggccccg tccggggccg acccggtgcg ccaccccgaa cgcgcgcggc
     2641 gtgcccgcgc gcgcgtgctc gaccgcatgg tcgccgaggg gctggtcggc cgggcggagg
     2701 ccgaggcggt gcgccgcgcg gacctcggcg tggtggagac gccgccggac tggcgcgcgc
     2761 cgtggttcgt cgaccacgtg ctcgacacgc tgcgggacga cgaccgcttc gccgcgctcg
     2821 gccaggacgc cgacgcgcgc ctggaccggc tgttccgcca gggcctgcgc gtggagacga
     2881 cgctggaccc ggtgtggcag gccgcggccg agcgcgccgt gcgcgaggtg gtgcacgagc
     2941 ccggcgaccc gtacgcggcc gtggtggccg tcgacccgcg cgacggcgcg ctgcgcgccc
     3001 tggtgggcgg tcggcgctac ggcgacccgg gcgaccccgt cagccgcttc aacctggcca
     3061 cgcgcggccg acgccaggcg ggctcggcgt tcaagccgct ggtgctggcg gcggcgcttt
     3121 cgcgcgggca caccctggac gaccgcttcc cggccggccg gtccgtgtcc atcgccctgc
     3181 ccggcgacgt ggcgccgtgg gtggtccgca actacgacga gcgcgcgtac ggggagctgg
     3241 ccctgcgcga cgcgacacgg ctgagcgtga acacggtcta cgcgcggctg gtcatggacg
     3301 tgggcgcgga ggccgtcgcg gagctcgcgc gcgtggccgg ggtgggccgg cccctgcgcc
     3361 cgtacccgtc gatcgcgctg ggcacgcagg aggtcacgcc gctggagatg gcggcggtgc
     3421 aggccacctt cgccgccgga ggcgtccgcc ggccggtggt ggccgtgcgc cgcgtcaccg
     3481 acgccggcgg ggcggtgctg tacgaggccg agccggcgcg cggccggcgg gtgctgccgg
     3541 agggcgtggc cgccctggtg acggaggccc tccaggacgt cgtgcgccgc ggcaccgggc
     3601 aggccgccgc gatcggcagg cccgtcgccg gcaagaccgg cacgacgcag aactgggcgg
     3661 acgcgtggtt cgccggctac acgcctcggc tggccgccgc ggtgtgggtc ggcttcccgc
     3721 aggcggccga gccgatgacc ccgccgcgca cccgcgagcg cgtggagggc ggcggctggc
     3781 ccgcggagat cttcgcggcg ttcgcggacg cggcgctgtc cggcgtcccc gccggcgagt
     3841 tcgccgcgcc ccccggcggg gtggtgcggg tggaggtgga ccgcacgcgc aactgcctgc
     3901 ccaacgccta caccccgcag gagctggtcg agctgcgctc ctacgtgccc ggcagcgagc
     3961 ccaccgagca ctgcaccgag ccgagcggcc cgtacgacgc gcgcgtgccc gacgtggtgg
     4021 gcctgccgtt cgaggccgcg gcgcgcctgc tcggcggcga gggcctgctg gtggcgcgca
     4081 gggcggagct gcgagcggac ctgccgccgg gccgcgtggt gcgccagtcc cccgacgcgg
     4141 acagcccgct gtcaccggac cccgcgctgc gcccgccggt gacggtgtgg gtgtccgcgg
     4201 gcgcggaggt gaccgtgccg gacgtgctgg ggctggagct ggagcgcgcg gtcgccgagc
     4261 tggagggccg ggggctgctg gtgcaggtgc gccaggagtg tcccggcgcc ggacgggact
     4321 gctcgggggc gcgcctgcgc cccggcaccg tgtgggagca gtctcccgac gccggccgcc
     4381 gcgtgcattt cggcgcgcag gtcaccctct tcgcctaccc cgcctgaggg tggcgcttgt
     4441 acggcgaggc ccagggcgag ccggggacca cgaagcgcca gggcgtgtcg aagccggcgg
     4501 ccacgccggt gcgcggcgtc gccgccacct ggggccgcgc gccgtcgtcg gccaggtaca
     4561 gcacgccgcc ggagcacagg tccgcgccgc tccaggaccc gtccaggccg tacgcctgcg
     4621 ccagccgcgc cgggccgcgc agcaggtcgc ggtccctggc gccgccgcgg cgcgcgcgca
     4681 tcagctcggt gttctccagc ggctcggctg cgcgcagcag caccccctgg ccgtgacccg
     4741 gcgtgcccgt caccgtgttc aggcaccagt gcatgccgta ggtgaagtac acgtaggcgt
     4801 gcccgggcgg gccgaacagc ggcgcgttgc gcgccgtctg cccccggtag gcgtggcacg
     4861 ccgggtcgtc ctggaggtag gcctccacct cgacgatgcg cgccaccagg ccctggtcct
     4921 cgcgcaccag cagcttgccc accagctccg gcgccaccgc cgtcgccggg cgggcgaaga
     4981 aggcccgctc cagccgctcg gtcatgcgcc ggaccgttcg cgcttggcgc gcacgaacgc
     5041 gagcaggccg tccaggtccc ggcagttgag gatctgcgcc ggcgtggccc agccgcgctg
     5101 cgcggtcgcc acgccgtagc gcatcaggtc caggtcgccg acgcggtggg cgtcacagga
     5161 gatcgccagc gtggcgccgc cctcgacggc gcgccgcacc atgtcgccgg acaggtccag
     5221 gcgcgcgggg tgcgcgttga cctccagggc cgtgcccgtg cgcacggccg cctccaccac
     5281 cgcctccagg tcgatctcgt agccgggccg ccggccgatc ttgcgcccgg tggggtggcc
     5341 gatgacgttg accgccgggt gctccatcgc cctgacgatg cgcgccgtct gctcctcgcg
     5401 cggcctgggg aagtgcgagt gcacgctggc gaccgtgaag tcgtagccga gcaggaagtc
     5461 ctcgtcgtag tccagcgagc cgtcgatgcc gatgttcagc tcggcgccgt gcaggatgcg
     5521 catgccgggg aagcgctcct gcagccgggc gacctcctcg cgctgggcca gcatgcgctc
     5581 gcgcgacagc ccgttcatgg tgaggttctc ggcgtggtcg gtgagcgcgt agtaggcgta
     5641 gcccttcgcg gccgccgcct ccagcagctc ctccagggtc gccttgccgt cgccggacca
     5701 gtcgctgtgg ccgtgcaggt ccccgcgcac gtcctccagg cgcaccaccg ggggcaggcg
     5761 cccctccgcc gccgcgtcga cctcgccggt gtcctcccgc agcgtcggcg gcacccactg
     5821 caggcccagg gcggcgtaga cgtcctcttc cgtggcgccg gccaccagct cgccggactc
     5881 cttggcgaac agcccgtact cgttgagcgt caggccgcgg cgcacggcgc gttcgcgcac
     5941 gcgcacgttg tgctccttgg agccggtgaa gtaggccagc gccgcgccga actcctcgcg
     6001 cgcgaccacg cgcaggtcga cctgcagccc gcgcacggtg agcacgctgg tcttgcgctc
     6061 ccccgccgcg atgaccttga ccaccaggtc gctgtcgcgc agggccgcca tgacgggcgc
     6121 cgggccgtcg gcggacacca ggatgtcgac gtcgccgatg gtctcgcgca tgcgccgcag
     6181 gctgcccgcc gtctccaccc ggtggacgcc cggcagcggc gccaggcgcg cgcacagctc
     6241 gtcggcgacg gccagcgcct cctccacggg gatgcgcttg tcctgcttcg cgccggcgcg
     6301 ctccagcgac tcgcgcaggt tctgcacgct cttggggccc aggccgggga tgcccttgag
     6361 ctcgtcgcgc tccagcgcgg cacgcagcgc ctccacgctg tcgatgccga gcgcctcgtg
     6421 cagcgcgcgg atggtcttgg gccccaggcc ggggacgcgc accagctcgg tgaagcccgg
     6481 cggcacctgc gccttgagct cgtcgagcat gccgatggcg ccggtgtcgc ggaactcggc
     6541 gatcttcctc gcggtggcgg cgccgatgcc cttgagcttg gcgatctctg cgtcggagag
     6601 ctccgccagg tccacggggc atgccgagat cgccccggcg gcgcgctcgt aggcgcgcac
     6661 gcggaaaggg tccgcgccgt cgagcttgag cagggtggcg atctcggaga agcgccgcgc
     6721 cacctcgtcg ttgagccagg gcatgcccac aatgctaggt cgcgcgccgc cgtgcgggcc
     6781 gcgcggctag catcccgcca tgcccggccc cgactccgac cttggccacg acctcgtcgc
     6841 ccatctgtac gcggccgccc tgcggctgat gcacgagcac cagcaggcgt tcctgccgct
     6901 gaacaaggtg caggagcagc tcggctggga cgacgagtcc ctggggcgcg tggtggacca
     6961 ctgcgtccgg gaacggcccg ccatcacgct gccgggcgcg ccgagcgcgc tgaccgccga
     7021 ctggatggac gacggcgacc gcccggccta cggccgcagc ggcgtgcgct tcatcgggat
     7081 tcgtcccgca gcgccggggt gagcgccgcg gcgggctcga gcccttccgg gctgcgcgcg
     7141 ccgggcacca tcatgagcac cagcaccacg cccgcgccga ccccggacgc ggccagcagc
     7201 acctcccgcg ccccggaaac ctccgcgagc cggcccgcca ccacgaacga cagcggcatg
     7261 gcgcccaggg ccgccatcca gtccaggctc gacacgcggc cgagcagccg gcgcggcacc
     7321 tgccgctgca gcgtcgtggt ccacacgatg tcggtgaagg tgaacatcgc gttcaggagc
     7381 acggcggcga gcacgccctg ccacaggctg cgcatcgccc cgtacatcgc cagcagcgcg
     7441 atgccgagcg cctgggcgag gtacatggcg gtcatcgcgc gccgggggat gccgcgctgg
     7501 ccgaccagca gcgccatgag caccgaaccg ccgcctccgg cggccaggac ggcgcccagg
     7561 aagtaggcgg cctgccgctg cgtgagcccc aggtcgttct tgacgacgta cggcagcagc
     7621 atgtcgaccg gacccgtgaa ggccagcagg cccgcggccg aggcgatcag ccacacccag
     7681 caccagcggt ggctgcgcac gaagcgcagg ccctccgcga cggtgcgcag cgagcccggc
     7741 ggcgacgcgg ccgcgtcggc gtcaatgtca gcggtgcgcc gcggcaggcg ggacagcagc
     7801 gcggccgaca ccaggaacgt gccggcgtcg acgagaaacg ccgggcccgt gccgaacgtg
     7861 cccaccagca ggccgcccgc ggccggtccc agcagccgca ccatcgcggg ccgcgcgacg
     7921 cccaggaagg cgttcgcccg cgcgagctcc cccgtgggca gcaggtccgg gacgatggac
     7981 gtcatcgccg ggttgaagaa cgcgttggag gcgcccacca cggcccccac cacgccgaag
     8041 tgccacagct ccagcgagcc gttgagcgac agcgccccgg cgccgagcag cgccgcccct
     8101 cgcaggaggt ccgcggcgat catgatgcgg cggcgttcga agcggtcgac cgcccacccg
     8161 ccgaccagca gcatcagcag ctgggaggtg gtccacacgg cgccgacgac cgccatggcg
     8221 gtcgggatgt tgctgatggc gtagacctgc agcggcagcg cgaccaggaa caccccgtcg
     8281 ccgaacaggc tcaccagcga cccggccgcc aggcggcggt acgccgcgtg gcgcagcggg
     8341 cgcaggatcg cgggaaggct ccgctgccgc atcccgcccg gcccccgctc ctgatccacg
     8401 cgcttccctc gtcgtcccgc caacgtcagc caccgcgcgg cgcgaacgcg cgcggatcct
     8461 catgatgccc gctcgaaccg gctccgcgac agacacaaac gggggattga cgcctcctca
     8521 ccgcctgccg ggcgtcaaaa tcagccgtcc agcagcgctc gcaggcccgc gagctgtgcc
     8581 cgaaggggct ccggcgccgg tcccagcgag gcgccccggc ggaccaccgc ggcgtccggg
     8641 cgcagcaggt ccaatggtgc gtcctgtagc tccgggaaca ccgtgacgag attcttgcgc
     8701 ttgacctcgt cgagctccag gccgcgtcgc tcgcattcgc tgacgaaggc cccgacgcgc
     8761 tcgtgcgcct ggcggaacgg gacgccgcgc tgcaccagcg cctcggcgag gtcggtggcc
     8821 agcgcgaagc ccccgtgcgc ggcggcgcgc aggcggtcct tgtcgaaggc cagcgagcgc
     8881 acggtgccgg ccatggccgg caaggccacc cgcagggtgt cgacggcgtc gaacaccggc
     8941 tccttgtcct cttggaggtc ccggttgtac gtcaggggca ggcccttgag cgttaccagg
     9001 aggctgacca ggtcgccgat gagccggccg gtcttgccgc gcacgagttc ggccacgtcc
     9061 gggttgcgct tctgcggcat gatcgaggag ccggtggaga acgcgtcgcc cacgcgcgcc
     9121 cagccgaact cggtggagct ccacagcacg atctcctcgc ccaggcgcga caagtggacg
     9181 cccgccatgg cggcggcggc caggaactcc acggcgaagt cacggtcggc cacggcgtcc
     9241 atggagttct cggccggccg atcgaagccg agcgcgtcgg cgtagcgggc ggggtccagg
     9301 ccgagcgtga ggcccgccag cgcgccggcg ccgagcacgg acacgtccat gcgccgcgcg
     9361 gcgtcggtca gccggcccgc gtcgcgctgc agcgcccagg cgtgcgcgag caggtgatgg
     9421 ccgagcgcga cgggctgggc ccgctgcagg tgcgtgtagc ccggcgcgat ccagtcgagg
     9481 acctcctcgg ccttgtcggc cagggctcgc tgcacggagc ggacgccgtc gagcagctcc
     9541 gcgatggcgt cgcgcaggta caggcgcagg tcgttgcaga tctggtcgtt gcggctgcgc
     9601 ccggcgcgca ggcggccgcc cgcctcgccg cacaactcca ccaggcggcg ctcgacggcg
     9661 ccgtgcaggt cctcgtcgtc gtcgaggaag gcgaacgtgg acgcctcgaa ctcgccggcc
     9721 acgacgtcca gcgcccccat gatcgccgcg tggtcctcgg ccgtcagcag ttccaggcgc
     9781 cgcagctcgt cggcgtgcgc gcgcgacccg gtgatgtcgt acggccacag ccgcgcgtcg
     9841 aagtggacgc tgcggccgag
//
LOCUS       DATMKC010000367         3891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_68320, whole genome shotgun sequence.
ACCESSION   DATMKC010000367 DATMKC010000000
VERSION     DATMKC010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68320"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VNU01_10785"
     CDS             complement(<1..936)
                     /locus_tag="VNU01_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HWH33142.1"
                     /translation="MAAQLSLADLGVALHQVPFVVVDLETTGGSARASAITEIGAVRI
                     QGGEVTGEFHTLVDPGLPIPASIAALTGITDAVVAGWPSIAGVLPSFLEFSRGAAFVA
                     HNAGFDLGFLNANLERLGYPRLDAPVVCTAALARRLVRDEVRNVKLATLAEFFRCRTR
                     PVHRALADARATVEVFHGLLERAGSFGVLTLEDLVQFSRVRNAPLFKARRGLADGLPQ
                     APGVYAFRSSSGEVLYVGKATDLRARVRQYFGSDDRRKIADLMKEAEAVDHWVCPTPL
                     EAAVRELRLIHAHRPRFNRRDKSPRRGVWLRLTDDA"
     gene            complement(1012..1257)
                     /locus_tag="VNU01_10790"
     CDS             complement(1012..1257)
                     /locus_tag="VNU01_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWH33143.1"
                     /translation="MRDANWALRAACRGQDVELFYSNEPTSVRAALRICGHCPVRKDC
                     FEDAVQRGEWFGVWGGLKESRRRRIVRQVAAEDADAA"
     gene            1383..2048
                     /locus_tag="VNU01_10795"
     CDS             1383..2048
                     /locus_tag="VNU01_10795"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH33144.1"
                     /translation="MTPSPQAPPLSTGDAPVTRNGATGRDVSLLDAASRRFVAVGIAK
                     TTMEDIAREAGAGKATLYRYFPNKAAVVDALVRRENERFTRRLTRAVTDGDGAAGKIE
                     AAFVAALDFLRSHPMLNKSLREEPDVLLPYLTLRSGPMVETGMRIFAGVIQEGVRSGE
                     LRPVHPEWAAETLFRLVMSFFSLPPLAIRVDDPEQVRQYAHGLVAGTLAARPTSPSRG
                     RGR"
     gene            2056..2865
                     /locus_tag="VNU01_10800"
     CDS             2056..2865
                     /locus_tag="VNU01_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWH33145.1"
                     /translation="MSVLLRDLDDGVCTLRLNRPEALNALSGEMATRLREALAEAEAD
                     DAVRCVVLTGAGRGFCAGADLGHIAAEFGSAAQAGGAPSSAVVLEAIRRHSVPLVDAL
                     LGFSKPLVAAVNGPCAGAGMGLALGADVIVAAESATFTVVFTRRGLVPDYGVTHLLPR
                     LVGLRLARQLCLLADAVPAGEALRMGLVSEVVADAELDAAAGRWARRFAEGPGVALRL
                     TKRLLAESFELDARQAVEREFAAQALAMTTADAIEGVAAFLDKRAPRFTGR"
     gene            2867..3364
                     /locus_tag="VNU01_10805"
     CDS             2867..3364
                     /locus_tag="VNU01_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWH33146.1"
                     /translation="MADRDRDELGRPRNARPRDRTGRPLSRGAPDAMADRQEPDEVVR
                     DIGDALRRAVDLVDGGRLFEAHEFLEWVWKSPDVAAADRDFWKGVTQVVVAGVHQQRG
                     NASGVARLASRGAALLRGYPPGHRGVDTVALLADAERLLAAADGPDPAEALRRVAPPR
                     LRLLA"
     gene            3446..3694
                     /locus_tag="VNU01_10810"
     CDS             3446..3694
                     /locus_tag="VNU01_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33147.1"
                     /translation="MDLAAGLEPGKRFRCGACGNLTRFDVLARERVRRFWHVDLSGEG
                     AADEEELLEREVESVACRWCGSAEHIEVVDAPGAAEAS"
BASE COUNT          495 a         1397 c         1502 g          497 t
ORIGIN      
        1 ggcgtcgtcg gtcagccgca gccacacgcc ccggcgcggc gacttgtcgc ggcggttgaa
       61 gcgggggcgg tgcgcgtgga tcagccgcag ctcgcgcacg gccgcctcca gcggcgtcgg
      121 gcagacccag tggtcgaccg cctcggcctc cttcatgagg tcggcgatct tgcggcggtc
      181 gtcggagccg aagtactggc gcacgcgggc gcgcaggtcc gtggccttgc cgacgtagag
      241 gacctcgccg gaggaggagc ggaacgcgta cacccccggc gcctgcggca gtccgtccgc
      301 gaggccgcgc cgcgccttga acagcggcgc gttgcgcacg cgcgagaact ggaccaggtc
      361 ctccagcgtg agcaccccga acgagccggc gcgctccagc agcccgtgga agacctccac
      421 ggtggcgcgg gcgtccgcca gcgcgcggtg caccggacgg gtgcggcagc ggaagaactc
      481 ggccagcgtg gcgagcttga cgttgcgcac ctcgtcgcgc accaggcgcc gcgcgagcgc
      541 cgccgtgcac accaccggcg cgtccaggcg cgggtagccc aggcgctcca ggttggcgtt
      601 caggaagccc aggtcgaagc cggcgttgtg cgcgacgaac gccgcgccgc gggagaactc
      661 caggaaggag ggcagcacgc ccgcgatgga gggccacccc gccacgacgg cgtcggtgat
      721 gccggtcagc gccgcgatgg acgcggggat cggcaggccg gggtccacca gggtgtggaa
      781 ctcgccggtg acctcgccgc cctggatccg caccgcgccg atctcggtga tcgcgctcgc
      841 gcgcgccgag ccgccggtgg tctccaggtc gaccaccacg aacggcacct ggtgcagggc
      901 gacgccgaga tcggcgagcg acagctgtgc ggccataagg ccgcgcagca taccgaacaa
      961 atgttcgcca cgccggcagg ccggccggtg cggcgcggac cggcccgtcc gtcaggcggc
     1021 gtcggcgtcc tccgcggcca cctgccgcac gatccggcgg cggcgggact ccttgagccc
     1081 gccccacacg ccgaaccact cgccgcgttg cacggcgtcc tcgaaacagt ccttgcgaac
     1141 cgggcagtgc ccgcagatgc gcagggcggc gcgcacgctg gtgggctcgt tggagtagaa
     1201 caactccacg tcttgccctc ggcacgcggc ccggagcgcc cagttggcgt ctctcatcgc
     1261 cccctccgtt ctccctgcgc cggacggccg ggcccgcgcg gcggtgcggg catcggcatg
     1321 gctcacacca cgcgcgcggt ggcggcggca cggggaaacg cgatccgtat actcgtctcg
     1381 ccatgacgcc atcgccgcaa gccccgcccc tgagcaccgg tgacgcaccg gtgaccagaa
     1441 acggcgccac tggcagggat gtctctctct tggacgccgc aagccgccgg tttgttgccg
     1501 tcggtatcgc aaaaacgact atggaggaca tcgcacgcga agcgggcgcg gggaaagcga
     1561 cgctgtaccg gtacttcccg aacaaggccg cggtggtcga cgcgctggtg cgccgggaga
     1621 acgagcgctt cacgcggcgc ctgacccggg cggtcaccga cggcgacggc gcggccggca
     1681 agatcgaggc cgccttcgtg gcggcgctgg acttcctgcg ctcccacccg atgctgaaca
     1741 agagcctgcg cgaggagccg gacgtgctcc tgccgtacct caccctgcgc tccgggccca
     1801 tggtggagac gggcatgcgc atcttcgccg gcgtcatcca ggagggcgtg cgctccggcg
     1861 agctgcgccc ggtgcatccg gagtgggccg ccgagaccct gttccgactg gtcatgagct
     1921 tcttcagcct tccgccactg gccatccgcg tggacgaccc ggagcaggtg cgccagtacg
     1981 cgcacggcct ggtggccggc acgctggcgg cgcggccgac gtcgccgtcg cgcgggcggg
     2041 ggcgctgagc agccgatgag cgtgctcctg cgcgacctcg acgacggcgt ctgcacgctg
     2101 cgcctgaacc ggccggaggc gctgaacgcg ctgtcgggcg agatggcgac gcggctgcgc
     2161 gaggccctgg cggaggccga ggccgacgac gcggtccgct gcgtggtgct cacgggcgcg
     2221 ggcaggggtt tctgcgcggg cgccgacctg ggccacatcg ccgccgagtt cggttcggcg
     2281 gcgcaggcgg gaggcgcgcc gagctcggct gtggtgctgg aggcgatccg gcgccactcc
     2341 gtgccgctgg tggacgcgct gctgggcttc tccaagccgc tggtcgccgc ggtgaacggt
     2401 ccgtgcgcgg gagccggcat gggcctggcg ctgggcgccg acgtgatcgt cgcggcggag
     2461 tcggccacgt tcacggtggt gttcacgcgc cgcgggctgg tgccggacta cggggtcacc
     2521 cacctgctgc cgcggctggt gggcctgcgg ctggcccggc agctgtgcct gctggccgac
     2581 gcggtgcccg cgggcgaggc gctgcgcatg gggctggtca gcgaggtcgt ggcggacgcg
     2641 gagctggacg ccgcggccgg ccgctgggcc aggcgcttcg ccgaggggcc cggcgtggcg
     2701 ctgcggctga ccaagcgcct gctggccgag agcttcgagc tggatgcccg ccaggcggtg
     2761 gagcgcgagt tcgccgccca ggcgctggcc atgaccacgg ccgacgcgat cgagggcgtg
     2821 gccgccttcc tggacaagcg cgcgccccgg ttcaccggcc gctgacgtgg ccgaccgcga
     2881 ccgcgacgag ctggggcggc cacgcaacgc caggccgcgg gaccgcaccg gccgccccct
     2941 gtcgcgcggc gcgcccgacg cgatggcgga ccgccaggag cccgacgagg tggtgcgcga
     3001 catcggcgac gccctgcgcc gggcggtgga cctggtcgac ggcggccggc tgttcgaggc
     3061 gcacgagttc ctggagtggg tgtggaagtc gcccgatgtg gcggccgccg accgcgactt
     3121 ctggaagggc gtgacgcagg tggtggtggc cggcgtgcac cagcagcgcg gcaacgcctc
     3181 cggggtggcg cgcctggcgt cgcgcggcgc ggccctgctg cgcggctacc cgccgggcca
     3241 ccgcggcgtc gacacggtgg cgctgctggc cgacgccgag cgcctgctgg ccgcggcgga
     3301 cgggccggac ccggccgagg cactgcgccg agttgcgccg ccccgcctcc ggctgcttgc
     3361 ctagcgtctg ctcacctagc atctgctcac agtgagcagt tcctccgata tcgcgccgcg
     3421 cgccgccccg aggagtgacg acgacatgga tctggccgcc ggcctcgagc ccggcaagcg
     3481 cttccgctgc ggcgcgtgcg gcaacctcac ccgcttcgac gtgctggcgc gcgagcgcgt
     3541 gcgccgcttc tggcacgtcg acctgtccgg cgagggcgcg gcggacgagg aggagctgct
     3601 ggagcgcgag gtcgagtccg tggcctgccg ctggtgcggc tccgccgagc acatcgaggt
     3661 cgtggacgcg ccgggcgccg ccgaggcgtc ctgacgccgc ggcggccggg ggactgtcac
     3721 agccgggtcg tacggtcgtc gcatgctgat cgactgtgat gcctgcgccc tgaaggacag
     3781 cgacgcctgc ggcgactgcg tggtgacctt cctggtcgag cgccccgagg gcgccatcgt
     3841 gttcgacgtg gaggaggaac gcgcgctgcg cagcctccag cgggcgggcc t
//
LOCUS       DATMKC010000368         5414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_162812, whole genome shotgun sequence.
ACCESSION   DATMKC010000368 DATMKC010000000
VERSION     DATMKC010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162812"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VNU01_10815"
     CDS             complement(<1..601)
                     /locus_tag="VNU01_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWH33148.1"
                     /translation="MLELPHGLRPGGRFAEWTATPPRYLVADVDGTLLAESHVPTQAV
                     LEAAAACGPAGLRVGVATGRMPHAVADLIARLGAEGPHVVHNGAEVWADGAILEAWPL
                     PPQARAVLRDLCAGHGLYAEFYAGDGYWVTDRRREAQRHWELLRRDPDGDAGALDLAR
                     TEVLKATVILFSGDDPAPVLATLAEAGLTCGAGSSPENRI"
     gene            651..1106
                     /locus_tag="VNU01_10820"
     CDS             651..1106
                     /locus_tag="VNU01_10820"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWH33149.1"
                     /translation="MALVEQIQSDLTAAMKARDTETTATLRMVMAAVKNARVAPGQQG
                     EISDAQALDILAKEAKKRAEAIEAYTQAGRQELADKEQAELAVIQRYLPQPLTEDEVR
                     AIVEEAIAATGASGPGDMGKVMGAVTPQTKGRADGRLVSTMVKDRLAGA"
     gene            complement(1223..1975)
                     /locus_tag="VNU01_10825"
     CDS             complement(1223..1975)
                     /locus_tag="VNU01_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH33150.1"
                     /translation="MHTEIEGPDGAPSLVLLHGGIGTGRSHWSKLVPALAERFRVYLP
                     DLPGHGRTPLAGGVDYGMGCHVGPVTDLLEQLGPPVHVAGFSMGGHVAMTIAADRPEL
                     FASLTAIGVSVREHAGLEKWRRQFEPETFEAANPLWSRYLSKVHAPLGGPDAWRDVLR
                     RDSTKVAAASRTAEDFADLTCPVLLVRGDRDWAVEPAQYAELRQLWGDRSEELVVPNG
                     GHDVQITRWRLVEPALLDFLDRAVKQAAEGRP"
     gene            2036..3154
                     /gene="ychF"
                     /locus_tag="VNU01_10830"
     CDS             2036..3154
                     /gene="ychF"
                     /locus_tag="VNU01_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWH33151.1"
                     /translation="MALQVGIVGLPNVGKSTLFNAVSAAGAEAANYPFATIEPNVGVV
                     ALRDERLDTLARLNRSAKVVPTSVEFLDIAGLVKGASQGEGLGNQFLSHIREVDAIVH
                     VVRCFDDPDIVHVDGSVDPVRDIEVIDTELILKDLETVEKRAQRSGKAARVGDKDGMR
                     EAEVVAALSAHLEGGSPARTFAFTEDDRVLRRELALLTDKPVLYAANVAESDLPDGNA
                     YVDQVRAVAEKEGATSTTGMSPGVIVVCAEAEAQISELPEDERAEFLSDLGLERSGLD
                     QLVHAAYRLLGLITFFTSGPQETRAWTVRQGTKAPQAAGKIHGDIERGFIRAETISYG
                     VYAELGGEQAVKDAGKLRVEGKDYIVQDGDVMHFRFNV"
     gene            complement(3206..3646)
                     /locus_tag="VNU01_10835"
     CDS             complement(3206..3646)
                     /locus_tag="VNU01_10835"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWH33152.1"
                     /translation="MRPVETSVEVARPAAEVFAFVSDVTNNPRFQKGMRSCEWTSPPP
                     VDEGSTYDQVAHFLGRDVVSKFVVDAYEPGRLIRFRSVAGPFPIVETRVVEPAGPDAC
                     RVEVTVGGSGSAFFKLADPLLRPMVAASVKRDYRRLAALLQPGG"
     gene            complement(3679..4089)
                     /locus_tag="VNU01_10840"
     CDS             complement(3679..4089)
                     /locus_tag="VNU01_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWH33153.1"
                     /translation="MRALLERSRVIAVVGCSADPAKPAHTVPRRMQQAGFRIIPVNPN
                     HAGQEVLGERCYASLADVPERVDLVNVFRRPQFTPDVVRQAVEVGAPAVFLQLGIAHP
                     ESRRIAEEAGIDYVEDACVAVERAKYAVTHSPGA"
     gene            complement(4095..5096)
                     /locus_tag="VNU01_10845"
     CDS             complement(4095..5096)
                     /locus_tag="VNU01_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWH33154.1"
                     /translation="MEQTYLGRTGLVVSELCLGTMTFGGAADEATSRAIVERFRAAGG
                     TFFDTADIYNAGASEQILGRIVAAERDQVVIATKGHGVTGPGPNDRMSSRRHLARAVD
                     DSLRRLGTDWIDLYQLHAWDPTTPLEETLSTLEDMVRAGKVLHVGVSNFTGWQIERAA
                     RIQEARGWDPIVALQPQWSLVERLIEHETLPAAYANGLGILAWSPLAAGFLTGKYRRE
                     ESREDIAGRGRFAQWVDNIDERGWAVLDAVREIADAHGVEPATVAVAWLLTRPRCVPI
                     VGATRPEQLDASLAAAALELTEQDVARLDELSAPRSGYPWDFGTWPGRPARPDWGAP"
BASE COUNT          791 a         1974 c         1953 g          696 t
ORIGIN      
        1 tgatcctgtt ctccggcgac gacccggcgc cgcaggtgag ccccgcctcg gcaagcgtcg
       61 ccagcaccgg cgccgggtcg tcgccggaga acaggatcac ggtcgccttg agcacctcgg
      121 tgcgcgcaag gtccagcgcg ccggcgtcgc cgtccgggtc ccggcgcagc agctcccagt
      181 ggcgctgcgc ctcccgcctg cggtcggtca cccagtagcc gtcgccggcg tagaactcgg
      241 cgtacaggcc gtgcccggcg cacaggtcgc gcaggaccgc gcgcgcctgc ggcggcagcg
      301 gccaggcctc caggatcgcg ccgtcggccc acacctcggc gccgttgtgc accacgtgcg
      361 ggccctcggc gccgaggcgc gcgatcaggt ccgcgaccgc gtgcggcatg cggccggtgg
      421 ccacgccgac gcgcaggccc gccgggccgc acgccgccgc ggcctccagc acggcctggg
      481 tgggcacgtg ggactcggcc agcagggtgc cgtcgacgtc ggccaccagg tagcgcggcg
      541 gcgtggcggt ccactcggcg aagcggccgc cggggcgcag tccatgggga agctcgagca
      601 tgtcggcccc gacgatacgg cacgggccgc gccctcctag aatcgccggc atggcactcg
      661 tcgagcagat ccagtcagac ctcaccgccg cgatgaaggc gcgtgacacc gagaccaccg
      721 cgacgctgcg gatggtgatg gcggccgtga agaacgcgcg cgtcgccccc ggccagcagg
      781 gcgagatcag cgacgcccag gcgctggaca tcctcgccaa ggaggcgaag aagcgcgccg
      841 aggccatcga ggcgtacacc caggcgggcc gccaggagct ggccgacaag gagcaggcgg
      901 agctcgcggt catccagcgc tatctgcctc agccgctcac cgaggacgag gtccgcgcca
      961 tcgtggaaga ggcgatcgcg gccaccggcg ccagcggccc gggcgacatg ggcaaggtca
     1021 tgggcgccgt gacgccccag accaaaggcc gcgccgacgg ccgcctcgtc agcacgatgg
     1081 tgaaggaccg cctcgcgggt gcctgacggt cctgcggcgc ggcccagtac gagtccgcca
     1141 ccccctctcg cgaggggccg cagacttcgc tagctgcccc gcgtcgccgg agtggccgtc
     1201 ccgcctcggt gcttgagcgc cgtcaaggcc tcccctcggc cgcctgtttc accgcgcggt
     1261 cgaggaagtc gaggagggcg ggctcgacga ggcgccagcg ggtgatctgc acgtcgtggc
     1321 cgccgttggg cacgaccagc tcttcgctcc tgtcgcccca tagctgtctg agctcggcgt
     1381 actgcgcggg ctcgacggcc cagtcgcggt cgccgcggac gagcaggacc gggcaggtga
     1441 ggtccgcgaa gtcctccgcc gtccgcgacg cggcggcgac cttggtggag tcgcggcgca
     1501 gcacgtcgcg ccaggcgtcc ggccctccca gcggcgcgtg caccttggac aggtagcgcg
     1561 accacagcgg gttggccgcc tcgaacgtct ccggctcgaa ctgcctgcgc cacttctcca
     1621 ggcccgcatg ctcacgcacg ctcacgccga tcgccgtgag cgaggcgaac agctccgggc
     1681 ggtccgccgc gatcgtcatg gcgacgtggc cgcccatgct gaagcccgcc acgtgcacgg
     1741 gcgggccaag ctgctccagc aggtcggtga ccgggccgac gtggcagccc atgccgtagt
     1801 cgacgccccc ggcaagcggc gtgcggccgt gcccgggcag gtcggggaga tagacccgga
     1861 agcgctccgc cagggcgggc accagcttgg accagtgcga acgcccggtg ccgatgccgc
     1921 cgtgcagcag caccaggctc ggcgcgccgt ccggcccctc gatctcggtg tgcatgccct
     1981 gcacgctacc ctggcgcgtc gctccccctt cacccagcga gaggttcgcg cgcacatggc
     2041 actgcaggtc ggcatcgtcg ggctgcccaa cgtgggcaag tccaccctgt tcaacgccgt
     2101 cagcgccgcc ggcgccgagg cggccaacta cccgttcgcc accatcgagc ccaacgtcgg
     2161 tgtggtggcg ctgcgcgacg agcgcctgga cacgctcgcg cgcctcaacc gctccgccaa
     2221 ggtggtgccc accagcgtgg agttcctcga catcgccggt ctggtgaagg gcgccagcca
     2281 gggcgagggc ctgggcaacc agttcttgag ccacatccgc gaggtcgacg cgatcgtgca
     2341 cgtggtgcgc tgcttcgacg accccgacat cgtgcacgtg gacggcagcg tggacccggt
     2401 gcgcgacatc gaggtcatcg acaccgagct catcctcaag gacctggaga cggtcgagaa
     2461 gcgcgcgcag cgcagcggca aggccgcgcg cgtcggcgac aaggacggca tgcgcgaggc
     2521 cgaggtcgtc gccgccctga gcgcgcacct ggagggcggc agccccgcgc gcacgttcgc
     2581 gttcaccgag gacgaccgcg tgctgcgccg cgagctcgcg ctgctcaccg acaagcccgt
     2641 gctgtacgcg gcgaacgtgg cggagtccga cctgcctgat gggaacgcct acgtcgacca
     2701 ggtgcgggcc gtcgccgaga aggagggcgc aacctcaacc acgggcatga gcccgggggt
     2761 catcgtggtg tgcgcggagg cggaggcgca gatcagcgag ctgcccgagg acgagcgcgc
     2821 ggagttcctg tcggacctcg ggctggagcg cagcggactg gaccagctcg tccacgccgc
     2881 ctaccggctg ctggggctga tcacgttctt cacgtccggg ccgcaggaga cccgcgcgtg
     2941 gaccgtgcgg cagggcacca aggcgccgca ggcggccggc aagatccacg gcgacatcga
     3001 gcgcggcttc atccgcgcgg agacgatcag ttacggcgtc tacgcggagc tgggcggcga
     3061 gcaggccgtg aaggacgccg gcaagctgcg cgtggagggc aaggactaca tcgtccagga
     3121 cggcgacgtc atgcacttcc gcttcaacgt ctagccgaat ggtcagatcg cttccgcata
     3181 cggaagcgat cctccgcatt gcacatcatc ctccaggttg gaggagggcg gcgaggcggc
     3241 ggtagtcgcg cttcaccgac gcggcgacca tggggcgcag cagcgggtcg gcgagcttga
     3301 agaacgcgct gcccgacccc ccgaccgtca cctcgacccg gcaggcgtcc gggccggccg
     3361 gctcgacgac gcgggtctcc acgatgggaa acgggcccgc cacgctgcgg aaccggatca
     3421 ggcgaccggg ctcgtaggcg tccacgacga acttggacac gacgtcgcgt cccaggaagt
     3481 gggcaacctg gtcgtaggtg gagccctcgt ccaccggagg cggcgacgtc cactcgcagg
     3541 agcgcatgcc cttctggaag cgggggttgt tcgtcacgtc ggagacgaac gcgaacacct
     3601 cggccgccgg ccgggcgacc tccaccgacg tctcgaccgg cctcatgcca ccctccccgt
     3661 cgccgcggac ggcgtcattc aagcacccgg cgaatgcgtg acggcgtact tggcgcgctc
     3721 caccgcgaca cacgcgtcct cgacgtagtc gatgcccgcc tcctcggcga tgcggcgcga
     3781 ctcggggtgg gcgatgccga gctgcaggaa caccgccggc gcgcccacct cgacggcctg
     3841 gcgcaccacg tccggggtga actggggccg ccggaacacg ttcaccaggt ccacccgctc
     3901 cggcacgtcg gcgagcgagg cgtagcagcg ctcgcccagc acctcctgcc cggcgtggtt
     3961 ggggttcacg gggataatgc ggaagccggc ctgctgcatg cggcgcggca ccgtgtgggc
     4021 gggcttggcg gggtcggcgg agcagcccac cacggcgatg acccgggagc gctccagcag
     4081 ggcgcgcatg tccgtcatgg cgcgccccag tccggccggg ccggacgccc cggccaggtg
     4141 ccgaagtccc acgggtagcc gctgcgcggc gcgctgagct cgtccaggcg cgccacgtcc
     4201 tgctccgtca gctccagcgc cgccgcggcc agcgacgcgt ccagctgctc gggccgcgtc
     4261 gcgccgacga tcgggacgca gcgcggccgc gtgagcagcc aggcgacggc cacggtcgcc
     4321 ggctccacgc cgtgggcgtc ggcgatctcg cgcaccgcgt cgagcaccgc ccagccgcgc
     4381 tcgtcgatgt tgtccaccca ctgggcgaag cggccccgcc cggcgatgtc ctcgcggctc
     4441 tcctcgcggc ggtacttgcc ggtgaggaac ccggcggcca gcggcgacca cgccaggatg
     4501 cccaggccgt tggcgtacgc ggcgggcagg gtctcgtgct cgatcagccg ctccaccagc
     4561 gaccactgcg gctgcagcgc cacgatgggg tcccagccgc gggcctcctg gatgcgcgcg
     4621 gcccgctcga tctgccagcc ggtgaagttg gacaccccca cgtgcagcac cttgccggcg
     4681 cgcaccatgt cctccagcgt cgacagggtc tcctccagcg gcgtggtcgg gtcccacgcg
     4741 tgcagctggt acaggtcgat ccagtccgtg ccgagcctgc gcaggctgtc gtccaccgcg
     4801 cgcgccaggt ggcgccggct ggacatgcgg tcgttgggac ccggccccgt cacgccgtga
     4861 cccttggtgg cgatcaccac ctggtcgcgc tcggcggcca cgatgcggcc caggatctgc
     4921 tcgctggcgc ccgcgttgta gatgtcggcg gtgtcgaaga acgtgccgcc cgcggcgcgg
     4981 aagcgctcga cgatcgcgcg cgaggtggcc tcgtcggcgg cgccgccgaa ggtcatggtg
     5041 cccaggcaca actcgctgac gacgaggccg gtccgcccga ggtaggtctg ctccatgcgg
     5101 ccagcatgcc ccccggcccg gcccgcgcaa aacggggccg cgcgtgtgcg caggcggcgg
     5161 cggtacgtac atacgcatac gtctttatcc gtactagggg ctagtcagga tcaatctgtc
     5221 gtggcacgat tggtctggca gccccccaga cgggggatgt aggtgccccc cacagggcgc
     5281 cgtggctggg ggaaccgggg aaatcacgct ggcggtcggt cagcgcgggc gaggtgggtc
     5341 cgccgcaaaa gatccaatgg catgcggtac tccacctgtt tttgcagggg tcacgcgttc
     5401 gcatgacctt gagc
//
LOCUS       DATMKC010000369        10728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_91784, whole genome shotgun sequence.
ACCESSION   DATMKC010000369 DATMKC010000000
VERSION     DATMKC010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91784"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2180)
                     /gene="glgX"
                     /locus_tag="VNU01_10850"
     CDS             complement(<1..2180)
                     /gene="glgX"
                     /locus_tag="VNU01_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWH33155.1"
                     /translation="MHQETQRVNVWPGRPYPLGATWDGRGTNFALYSESAEGVELCLF
                     DAEGHEARVAVRDHTAYIWHCYLPGVGPGQRYGYRVHGPWDPASGHRFNPHKLLLDPY
                     AKALVGELDWRHEVFGYRIGADDLTMDERDSAAHVPKSVVANPFFDWGDDRYPQVPWN
                     QTVIYETNVRQLTMLHPDIEPELRGTYAGIASPPVLDHLRRLGVTAIELEPVHHFVSE
                     HFLVEHGLRNAWGYNTVGFFAPHAPYAARGSLGEQVYEFKAMVKDLHAAGFEVILDVV
                     YNHTAEGNHQGPTLSLRGIDNRAYYRLVDGDERHYMDYTGTGNSLNARHPHVLQLIMD
                     SLRYWVLEMHVDGFRFDLASTLARELHDVDRLAAFFDIIQQDPVISQTKLIAEPWDVG
                     EGGYQVGNFPVLWTEWNGKYRDTMRDYWRGEIGGLGEFAARLSGSADLYEHTGRRPYA
                     SINFVTAHDGFTLADLTAFNHKHNEANPGDGGGEDHNRSWNCGEEGPTTDPAVTALRW
                     RQRRNFLATLLLSQGVPMLLGGDEFGRSQQGNNNAYCQDNEITWYDWKWDTDQEELFA
                     FTRRLVRLRARHPVFRRRQFFRGEGPGRTDDVEWFRPDGEAMTDEDWAFQGARTLGMF
                     LNGRAIPTRDERGRRILDDSFLLLFHSGHEAQAFTLPSAPLGERWGVVLDTARPALRE
                     RVEQFGAEGSVNACASWPEWNSSRKLSSRMRRPRSSRVGMARPL"
     gene            2215..3315
                     /locus_tag="VNU01_10855"
     CDS             2215..3315
                     /locus_tag="VNU01_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH33156.1"
                     /translation="MRPAQPSSAPRPARRDWLGPGIVAAAGLSVASGFAQFAATATLG
                     DVAAAFGETATGDSLVAQAGLAGSTLGIGLAVVRLASLASLPLAELADRRGRRGVLLG
                     VAAAGLGFRTVYALAVIPLLGVPLLARALPEPERYTRLRTQLTHGPRPRLSALGHVPR
                     ALRPTLLLLATLTAALAFVSGPVNSLLFLYAERIVGLSSSVTAVVVVAAGPVGLAGLL
                     AGRWAADRWGRRLTAGVSQAVVALAGVATYRGGGAFTIAGYLLSICASGAFGPAAAAL
                     AAELFPTSMRATAAGWLTVSGVLGAVSGLVAFGVLADAAGFALAALAVGVPVALITPL
                     YARLPETRGLELEQSAPEPEAEDGREPPPERG"
     gene            complement(3312..5276)
                     /locus_tag="VNU01_10860"
     CDS             complement(3312..5276)
                     /locus_tag="VNU01_10860"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWH33157.1"
                     /translation="MTSAAATAILVLLVRAYGQPAVQPSRGATWLFAFFVLLGELVPV
                     HVLRRGDGGETTIATPFALGLLVTAGVVDATVILAASTVVAGMVRQKPWGELAASAAR
                     YGLGLAAAGAAFELLHGRTFDSFGRVDELLAITLAAGVFFLVEHTLRARHLALTRGGT
                     VADRLRRDAAFQLAIVASQLAMTPVAALAAQRSVWMVPLLLVPAVAVHRSANLSLERE
                     QEALHDPLTRLPNRGLFHLRVEEALEVADGPVAVLFLDLDRLREVNDTLGHHIGDRLL
                     QQVGPRLRAVLGPAVTVGRIGGDEFAVLLPGVRDGRHAVRTADALAAAVAEPFRVKEL
                     SLAVEANIGVAVYPDHADDAAELMQRADVALYVAKERRSPTELYDADRGARSRRSLSL
                     VGDLRRGIDRRELVLHYQPKAELATSRIVGVEALVRWNHPDHGLVRPDEFIPLAEHTV
                     LMDPLTMWVLDEALSQCAIWREAGLDLRMAVNLSVRNLHDPQLAAKVAGRLHARGLPP
                     SCLELEITESSIMAEPTRAVETLSQLARLGVRLSVDDFGTGYSSLAYLKRLPVQEVKI
                     DRSFISEMATDANDTAIVRSAIELSRHLGLEVVAEGVETRETWEALAELGCHYAQGDY
                     LSSPLPPAEAIRALRGPVLAARGDAGADGA"
     gene            complement(5469..6110)
                     /locus_tag="VNU01_10865"
     CDS             complement(5469..6110)
                     /locus_tag="VNU01_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH33158.1"
                     /translation="MKVSSIPLPQPPSGRKALATYESLLAAAERVLAADGLPGLNSTV
                     VAAQAGVATGTFYTYFADKDALLAALFARRLDDISAAVEAALAGEALTAGGLEPALRG
                     AVRAVLQGYRAHATVIRAALARIPDSPLLRAVYWERHQRASAEVGRFLRRGAAAGVVR
                     RGGHQSLSLAMLVIVQGLNHPVVLAGEPRQVAAVSDHLVRALAGLLRPDPAAS"
     gene            6162..7757
                     /locus_tag="VNU01_10870"
     CDS             6162..7757
                     /locus_tag="VNU01_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWH33159.1"
                     /translation="MDVLGTRVDPSSEGYIQNRDGMLALLGQLDDALAQARAGGGEKY
                     VQRHRDRGRLLPRERVELLLDRDSPFLELSSLTAYGTEYHVGGSGVSGIGVVSGVECL
                     IGASDPTVKGGATNPFGLKRALRSMEIAMENRLPLINLTESAGADLPKQAEIFVPGGE
                     TFRNLTRMSAARVPTLSLVFGPSTAGGAYVPGMSDYTVMVRDQARVYLGGPPLVKMAT
                     GEDADEESLGGAEMHSTTSGVSDYLAEDELDAIRIGRQIVAHLNWRKLGPGPSLPADE
                     PVHDPEDLLGIGSVDVRVPFDVREVIARITDGSRFEEFKPRYGKTLVTGWASIHGYPV
                     GILGNNGILFSPESQKGAQFIQLCNQRDIPLVFLQNITGFMVGTAYEQGGIVKDGAKL
                     INAVANSKVPHFTIMIGASYGAGNYGMCGRAYGPRFLFTWPNHRIAVMGPQQLAGVMS
                     IVAENAAKKAGAAFDHDAAKAMQDAFQAEIESQSTAFYATARLWDDGIIDPRDTRTVL
                     GLALSAAHTNEVKGTPGYGVFRM"
     gene            7769..9799
                     /locus_tag="VNU01_10875"
     CDS             7769..9799
                     /locus_tag="VNU01_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWH33160.1"
                     /translation="MPEPRPITKLLVANRGEIACRVMRTARAMGIATVAVCSDPDRDA
                     THVREADEAVALGGAAASESYLVVDKLLDAARRTGADAVHPGYGFLAENAAFAQACAD
                     AGLVFVGPSPEVIAAMGDKLRAKELMERAGVPVLRSVAVTGEEVPSLDGVPFPVLVKA
                     SAGGGGKGMRIVREPSELADAVAAARREAASAFGDDTVFIEPYVERPRHIEVQILGDA
                     HGTVLHFGERECSIQRRYQKVIEEAPSPLVDDELRARLTSAAIDGAKALGYTNAGTVE
                     FIATQGGEFFFLEVNTRLQVEHPVSELVWRLDDAETEPVDLVRLQLLVAMGRPLPFAQ
                     DDLELVGHAIEARLYAEDPASGFLPATGTLALWEPAVLPGVRFDAGVHTGFEVGVNYD
                     PLLAKVIAHAGSRTEAALLLARALDATRVHGVTTNRSFLSAALRHPSFLSGDLHTGFI
                     DEHGLAAARPLGEEALLVSVAAAALAGVHARRRAGALPVPASVPSGWRNNRSQPQRIA
                     FDVPGREEPADAGYWLRRDGVWEVSLDERVAEVRVRAWPGQDEALDLDVDGIRRRAHV
                     HADGDMRYVDSPLGAIALRERPRFPVAGQEEVAGGLQAPMPGNVLQVAVAEGDEVAKG
                     DLLVILEAMKMEHRIAAPFDGTVTEVRVAQGQQVDADAVLVVLEAAGEAQAP"
     gene            9917..10321
                     /locus_tag="VNU01_10880"
     CDS             9917..10321
                     /locus_tag="VNU01_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWH33161.1"
                     /translation="MHEHPAARTALAAMQAVTRGDREAWLACYRDDAVLHDPVGGSPL
                     DPGGAGLRGTRALQDFWDLTIAPNDVAFTVAAVHPAGDEAAVVASVTIRFPGGNAVSY
                     DGVFVYRVDEAERVASVRAYWDLQRVLSALGA"
     gene            complement(10291..>10728)
                     /locus_tag="VNU01_10885"
     CDS             complement(10291..>10728)
                     /locus_tag="VNU01_10885"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HWH33162.1"
                     /translation="CGPQPPNVRLLPFAPQREILSRCALAVHHGGFSSTMECLSAGAP
                     QLVVPLTGDQGSHADAVTRHGLGSVLRHALGQGAFGVPLTDPARLDPQQVLTEARALA
                     ADPAVAGRVADFRRRVDELPGPPEFVAFVEELAGQAPRADRTR"
BASE COUNT         1398 a         3918 c         3981 g         1431 t
ORIGIN      
        1 ctcaacggcc gcgccatccc gacgcgcgac gagcgcggcc ggcgcatcct ggacgacagc
       61 ttcctgctgc tgttccactc cggccacgag gcgcaggcgt tcacgctgcc ctccgcgccg
      121 aactgctcga ctcgttcgcg cagtgccggc cgcgccgtgt ccagcacgac gccccaccgc
      181 tcgcccagcg gcgcggaggg cagcgtgaac gcctgcgcct cgtggccgga gtggaacagc
      241 agcaggaagc tgtcgtccag gatgcgccgg ccgcgctcgt cgcgcgtcgg gatggcgcgg
      301 ccgttgagga acatgcccag cgtgcgcgcg ccctggaacg cccagtcctc gtccgtcatc
      361 gcctcgccat ccggccggaa ccattccacg tcgtcggtgc gtcccggccc ctcgccgcgg
      421 aagaactgcc gccggcggaa caccgggtgg cgggcgcgca gccgcaccag ccgccgggtg
      481 aacgcgaaca gctcctcctg gtcggtgtcc cacttccagt cataccaggt gatctcgttg
      541 tcctggcagt aggcgttgtt gttgccctgc tggctgcgcc cgaactcgtc gccgccgagc
      601 agcatcggca ccccctggct gagcagcagc gtcgccagga agttgcgccg ctggcgccag
      661 cgcagcgcgg tgacggccgg gtcggtggtg gggccctcct cgccgcagtt ccaggagcgg
      721 ttgtggtcct cgccgccgcc gtcgccgggg ttcgcctcgt tgtgcttgtg gttgaacgcg
      781 gtgaggtcgg cgagcgtgaa gccgtcgtgc gcggtgacga agttgatcga cgcgtacggg
      841 cggcggccgg tgtgctcgta caggtccgcc gagccggaca ggcgcgccgc gaactcgccc
      901 agcccgccga tctccccccg ccagtagtcg cgcatggtgt cgcggtactt gccgttccac
      961 tcggtccaca ggacggggaa gttgccgacc tggtagccgc cctcgcccac gtcccacggc
     1021 tcggcgatga gcttggtctg ggagatcacc gggtcctgct ggatgatgtc gaagaaggcg
     1081 gccagccggt cgacgtcgtg cagctcgcgg gcgagcgtgg aggcgaggtc gaagcggaag
     1141 ccgtcgacgt gcatctccag cacccagtag cgcagggagt ccatgatgag ctggagcacg
     1201 tgcgggtggc gggcgttcag cgagttgccg gtgccggtgt agtccatgta gtggcgctcg
     1261 tcgccgtcca ccagccggta gtaggcgcgg ttgtcgatgc cgcgtagcga cagcgtgggc
     1321 ccctggtggt tgccctcggc ggtgtggttg tagaccacgt cgaggatcac ctcgaagccg
     1381 gcggcgtgca ggtccttcac catcgccttg aactcgtaga cctgttcgcc caggctgccg
     1441 cgcgccgcgt acggcgcgtg cggcgcgaag aagccgacgg tgttgtagcc ccaggcgttg
     1501 cgcagcccgt gctcgaccag gaagtgctcg gagacgaagt ggtgcaccgg ctccagctcg
     1561 atcgccgtca cgcccagccg gcgcaggtgg tccagcacgg gcggcgacgc gatgccggcg
     1621 taggtgccgc gtagctccgg ctcgatgtcc gggtgcagca tggtgagctg gcgcacgttg
     1681 gtctcgtaga tcacggtctg gttccacggc acctgcgggt agcggtcgtc gccccagtcg
     1741 aagaacgggt tggcgaccac ggacttgggc acgtgggcgg cgctgtcgcg ctcgtccatg
     1801 gtgaggtcgt ccgcgccgat gcggtagccg aacacctcgt gccgccagtc cagctcgccg
     1861 accagcgcct tggcgtaggg gtccagcagc agcttgtgcg ggttgaagcg gtggccgctc
     1921 gccgggtccc agggcccgtg gacgcggtag ccgtagcgct ggccgggacc gacgcccggc
     1981 aggtagcagt gccagatgta ggcggtgtgg tcgcgcaccg cgacgcgggc ctcgtggccc
     2041 tcggcgtcga acaggcacag ctcgacgccc tccgcgctct cactgtagag ggcgaagttg
     2101 gtgccacggc cgtcccaggt ggcccccaag ggatagggcc tgcccggcca cacattcacg
     2161 cgctgggtct cctggtgcac ggtgaggaca gggtgcgggc gtaggctgcc cgccatgcgt
     2221 ccggcccaac cgtcttcagc gccaagaccc gcccggcgcg actggctggg cccgggcatc
     2281 gtggccgccg ccggcctgtc ggtggcttcc ggattcgcgc agttcgccgc caccgccacg
     2341 ctgggcgacg tcgccgcagc cttcggcgag acggccaccg gggactcgct ggtcgcccag
     2401 gccgggctgg cgggcagcac gctgggcatc ggcctggccg tcgtccgcct cgcgtcgctg
     2461 gcgagcctgc cgctggccga gctcgcggac cgccgcgggc gccggggggt cctgctgggc
     2521 gtcgcggcgg cgggcctggg attccgcacg gtgtacgccc ttgccgtgat cccgctgctc
     2581 ggtgtgccgc tgctcgcccg cgccctgccg gagccggagc gctacacgcg gctgcgcacg
     2641 cagctcaccc acgggccgcg cccccggctg tccgcgctgg gccatgtgcc tcgggcgctg
     2701 cggccgacgc tgctgctgct ggcgacgctc accgcggcgc tcgcgttcgt gagcgggccg
     2761 gtcaacagcc tgctgttcct gtacgcggag cgcatcgtgg gcctgtcgtc gtcggtcacc
     2821 gccgtggtgg tggtcgccgc gggcccggtt ggtctggcgg ggctgctcgc cgggcgctgg
     2881 gcggcggacc gctgggggcg ccgcctcacc gccggcgtca gccaggccgt ggtcgcgctg
     2941 gccggcgtgg cgacctaccg cggcggcggc gcgttcacga tcgccggtta cctgctgtcc
     3001 atctgcgcgt ccggcgcgtt cggccccgcg gcggccgcgc tggcggccga gctgttcccc
     3061 acgtcgatgc gcgccaccgc ggccggctgg ctgaccgtgt ccggggtgct gggcgcggtg
     3121 tcggggctgg tggccttcgg cgtgctggcc gacgccgccg ggttcgcgct cgccgcgctg
     3181 gccgtgggtg tgcccgtggc gctgatcacg ccgctgtacg cgcggctgcc ggagaccagg
     3241 ggcctggagc tggagcagtc cgcgccggaa ccggaggccg aggacgggcg cgagcccccg
     3301 ccggagcgag gctaggcgcc gtccgcgccc gcgtcgccgc gggcggccag caccggcccg
     3361 cgcagcgccc ggatcgcctc ggcgggcggc agcggactgg acaggtagtc gccctgcgcg
     3421 tagtggcagc cgagctcggc cagcgcctcc caggtctcac gcgtctccac gccctccgcc
     3481 accacctcca gcccgaggtg gcgcgacagc tcgatcgcgg agcgcacgat cgccgtgtcg
     3541 ttggcgtcgg tcgccatctc gctgatgaac gagcggtcga tcttgacctc ctgcaccggc
     3601 aggcgcttga ggtaggccag cgaggagtag ccggtcccga agtcgtccac ggacaggcgc
     3661 acgcccaggc gcgcgagctg cgacagcgtc tccaccgccc gcgtcggctc ggccatgatc
     3721 gagctctcgg tgatctccag ctccaggcag gatggcggca gcccgcgggc gtgcagccgg
     3781 cccgccacct tggccgcgag ctgcgggtcg tgcaggttgc gcacggacag gttgaccgcc
     3841 atgcgcaggt ccaggcccgc ctcgcgccag atggcgcact ggctgagcgc ctcgtccagc
     3901 acccacatgg tgagcgggtc catgagcacg gtgtgctccg ccagggggat gaactcgtcc
     3961 gggcgcacca gcccgtggtc cgggtggttc cagcgcacca gcgcctccac gccgacgatc
     4021 cgcgacgtcg ccagctcggc cttcggctgg tagtgcagca ccagctcgcg ccggtcgatc
     4081 ccccggcgca ggtcgccgac caggctgagg cttcggcggc tgcgggcacc gcggtcggcg
     4141 tcgtacagct cggtggggct gcggcgctcc ttggccacgt acagggcgac gtcggcgcgc
     4201 tgcatcagct ctgcggcgtc gtcggcgtgg tcggggtaga ccgccacccc gatgttggcc
     4261 tccaccgcca gcgacagctc cttcacgcgg aacggctcgg ccacggcggc ggccagcgcg
     4321 tcggcggtgc gcacggcgtg ccggccgtcg cggacaccgg gcagcagcac cgcgaactcg
     4381 tcgccgccga tgcgcccgac cgtgacggcg ggaccgagca ccgcgcgcag gcgcgggccg
     4441 acctgctgga gcaggcggtc gccgatgtga tggccgagcg tgtcgttgac ctcgcgcagg
     4501 cggtcgaggt ccaggaacag caccgcgacc ggcccgtcgg cgacctccag cgcctcctcg
     4561 acgcgcaggt ggaacagccc gcggttgggc agccgcgtca gcgggtcgtg cagcgcctcc
     4621 tgctcgcgct ccagggacag gttcgcgctg cggtgcaccg cgacggcggg caccagcagc
     4681 agcggcacca tccacacgct gcgctgcgcg gccagggccg ccacgggagt catggcaagc
     4741 tggctggcca cgatggcgag ctggaacgcg gcgtcgcggc gcagccggtc ggcgacggtg
     4801 ccgccccggg tcagcgcgag gtgccgggcc cgcagcgtgt gctcgaccag gaagaacacg
     4861 ccggccgcca gagtgatggc gagcagttcg tccacgcgcc cgaaggagtc gaacgtgcgc
     4921 ccgtgcagca gctcgaaggc ggctccggcg gcggcaagac cgaggccgta gcgcgccgcg
     4981 ctcgccgcca gctcacccca cggcttctgc cgcaccatcc cggccaccac cgtggaggcg
     5041 gcaaggatca ccgtggcgtc caccaccccg gcagtcacga gcaggcccaa cgcgaacggc
     5101 gtggcgatgg tcgtctcgcc gccgtcgccg cggcgcagca cgtggacggg caccagctcg
     5161 cccaggagca cgaagaacgc gaacagccag gtggcgccgc ggctgggctg gacggcgggc
     5221 tgcccgtagg cgcgcacgag cagcacgagg atcgcggtcg ctgccgcgct cgtcacgagc
     5281 agctctgcaa gcacccgccc tcggagggcc gggtgcccgc gacggtgcgc catcatcggt
     5341 ggaacggtca cttccccacc tgactgccgt ccccgtgccc gcggtccccc ggtccggggg
     5401 acggcccgtt cacggtaaca acgatccggc gtcacgtcgc cactttttcg tggcggcgag
     5461 atccggcgct acgaggcggc cggatccggc cgcagcagcc cggccagcgc ccggaccagg
     5521 tggtcgctga ccgcggcgac ctggcgcggc tcgccggcca gcacgacggg gtggttcaag
     5581 ccctgcacga tcacgagcat cgccagggac aggctctggt ggccgcctcg ccgcaccacg
     5641 ccggccgcgg cgccgcgccg taggaagcgg ccgacttccg cgctcgcccg ctgatggcgc
     5701 tcccagtaca cggcgcgcag gagcgggctg tccgggatgc gcgccagcgc ggcgcggatc
     5761 accgtggcgt gcgcgcggta cccctgcagc accgcgcgga ccgcgccgcg cagggccggc
     5821 tccaggcccc cggcggtcag cgcctccccc gccagcgccg cctcgaccgc cgcgctgatg
     5881 tcgtccaggc gccgggcgaa cagcgccgcc agcagcgcgt ccttgtccgc gaagtaggtg
     5941 tagaaggtgc cggtcgcgac gcctgcctgc gcggcgacca ccgtggagtt cagcccgggc
     6001 aggccgtcgg ccgccagcac acgctccgcc gcggccagca gggactcgta ggtggccagc
     6061 gccttgcgac cggacggcgg ctgcggcaac ggaattgaag aaaccttcat atttgacgag
     6121 tatagtgccg ggcgacgcga cgaccgcggg aggagaagcc agtggacgtg ctgggcacgc
     6181 gcgtggaccc ctcgagtgag ggctacatcc agaaccgcga cggcatgctc gcgctgctcg
     6241 gccaactcga cgacgcgctg gcgcaggcgc gcgccggcgg cggcgagaag tacgtccagc
     6301 gccaccgcga ccgcggccgg ctgctccccc gggagcgcgt cgagctgctg ctggaccgcg
     6361 actcgccctt cctggagctg tcctcgctca ccgcgtacgg caccgagtac cacgtcggtg
     6421 gcagcggcgt gtcaggcatc ggcgtggtca gcggggtcga gtgcctgatc ggggcgagcg
     6481 accccaccgt caagggcggc gcgaccaacc cgttcggcct caagcgcgcg ctgcgctcca
     6541 tggagatcgc catggagaac cgcctgccgc tgatcaacct gaccgagtcg gcgggcgcgg
     6601 acctgcccaa gcaggcggag atcttcgtcc ccggcggcga gacgttccgc aacctgacgc
     6661 gcatgtccgc cgcgcgcgtg ccgacgctgt cgctggtgtt cggcccgtcc acggcgggcg
     6721 gcgcgtacgt gccgggcatg agcgactaca ccgtgatggt gcgcgaccag gcgcgcgtct
     6781 acctgggcgg cccgccgctg gtgaagatgg ccaccggcga ggacgccgac gaggagtcgc
     6841 tcggcggcgc ggagatgcac tcgaccacct ccggcgtgtc cgactacctg gccgaggacg
     6901 agctcgacgc catccgcatc ggccgccaga tcgtcgccca cctgaactgg cgcaagctgg
     6961 ggcccggccc gtcgctgccc gccgacgagc cggtccacga cccggaggac ctgctgggca
     7021 tcggctcggt ggacgtgcgc gtgccgttcg acgtgcgcga ggtgatcgcc cgcatcaccg
     7081 acggcagccg cttcgaggag ttcaagccgc gctacggcaa gacgctggtc accgggtggg
     7141 cgtccatcca cggctacccc gtgggcatcc tgggcaacaa cggcatcctg ttcagcccgg
     7201 agagccagaa gggcgcgcag ttcatccagc tgtgcaacca gcgcgacatc ccgctggtgt
     7261 tcctgcagaa catcaccggc ttcatggtcg gcaccgccta cgagcagggc ggcatcgtca
     7321 aggacggcgc caagctgatc aacgccgtgg ccaactccaa ggtgccgcac ttcacgatca
     7381 tgatcggcgc gtcctacggc gccgggaact acggcatgtg cgggcgcgcg tacgggcccc
     7441 gcttcctgtt cacgtggccc aaccaccgca tcgcggtcat ggggccccag cagctcgccg
     7501 gcgtgatgtc catcgtggcg gagaacgccg cgaagaaggc cggcgccgcc ttcgaccacg
     7561 acgccgccaa ggcgatgcag gacgcgttcc aggcggagat cgagtcccag tccacggcgt
     7621 tctacgcaac cgcccgcctc tgggacgacg gcatcatcga cccgcgcgac acgcgcaccg
     7681 tcctggggct ggcgctgtcc gccgcccaca ccaacgaggt caagggcacg ccgggctacg
     7741 gcgtgttccg catgtgaagg cggtccccat gcccgagccc cggccgatca ccaagctgct
     7801 cgtcgccaac cgcggcgaga tcgcctgccg tgtcatgcgc accgcgcgcg cgatgggcat
     7861 cgccaccgtc gccgtctgca gcgaccccga ccgcgacgcg acgcatgtgc gcgaggcgga
     7921 cgaggccgtc gcgctgggcg gcgcggcggc gtcggagtcc tacctggtcg tggacaagct
     7981 cctggacgcc gcgcgccgca ccggcgcgga cgccgtgcac cccggctacg gcttcctggc
     8041 ggagaacgcc gcgttcgcgc aggcgtgcgc cgacgcgggg ctcgtgttcg tcggtccttc
     8101 ccccgaggtc atcgccgcca tgggcgacaa gctgcgcgcc aaggagctga tggagcgcgc
     8161 cggcgtgccg gtcctgcgct cggtcgccgt gaccggcgag gaggtcccgt cccttgacgg
     8221 cgtgccgttc cccgtgctgg tcaaggcctc cgcgggcggg ggcggcaagg gcatgcgcat
     8281 cgtgcgcgag ccgtcggagc tggccgacgc cgtggccgcc gcacggcgcg aggcggcgtc
     8341 ggcgttcggc gacgacaccg tgttcatcga gccctacgtc gagcgcccgc gccacatcga
     8401 ggtgcagatc ctcggtgacg cccacggcac ggtgctgcac ttcggcgagc gcgagtgctc
     8461 catccagcgc cgctaccaga aggtcatcga ggaggcgccg tcgccgctgg tggacgacga
     8521 gctgcgcgcg cggctgacgt ccgcggccat cgacggcgcg aaggcgctgg gctacaccaa
     8581 cgcgggcacc gtggaattca tcgcgaccca agggggggag ttcttcttcc tggaggtcaa
     8641 cacgcgcctg caggtggagc accccgtcag cgagctggtc tggcgcctgg acgacgcgga
     8701 gacggagccg gtcgacctgg tgcgcctgca gctgctggtg gcgatgggcc ggccgctgcc
     8761 gttcgcgcag gacgacctgg agctggtcgg ccacgccatc gaggcgcgcc tgtacgccga
     8821 ggaccccgcg agcggcttcc tgcccgccac gggcacgctg gcgctgtggg agcccgcggt
     8881 cctgccgggc gtgcgcttcg acgccggcgt gcacacgggc ttcgaggtcg gcgtcaacta
     8941 cgacccgctg ctggcgaagg tgatcgcgca cgcgggcagc cgtacggagg cggcgctgct
     9001 gctggcgcgg gcgctggacg ccacgcgcgt gcacggcgtc accacgaacc ggtcgttcct
     9061 cagcgcggcg ctgcgccacc cctcgttcct gtccggcgac ctgcacaccg gcttcatcga
     9121 cgagcacggc ctcgccgcgg cgcggcccct cggcgaggag gcgctgctgg tctccgtggc
     9181 ggcggccgcg ctggccggcg tgcacgcgcg ccgccgcgcc ggcgcgctgc cggtgccggc
     9241 gagcgtgccg agcggctggc gcaacaaccg ctcgcagccc cagcgcatcg ccttcgacgt
     9301 cccgggccgc gaggagcctg cggacgccgg ctactggctg cgccgcgacg gcgtctggga
     9361 ggtgtcgctg gacgagcgcg tcgccgaggt gcgcgtgcgg gcctggcccg gccaggacga
     9421 ggcgctggac ctcgacgtgg acggcatccg ccggcgcgcg cacgtgcacg cggacgggga
     9481 catgcgctac gtcgactcgc cgctgggcgc catcgcgctg cgtgagcgtc cgcgcttccc
     9541 ggtggcgggc caggaggagg tggccggcgg gctccaggcg ccgatgccgg gcaacgtcct
     9601 gcaggtggcc gtcgccgagg gcgacgaggt cgcgaagggc gacctgctgg tgatcctcga
     9661 ggcgatgaag atggagcacc gcatcgccgc gccgttcgac ggcacggtga cggaggtgcg
     9721 cgtggcccag ggccagcagg tcgacgccga cgcggtcctg gtcgtgctgg aggccgccgg
     9781 cgaggcccag gccccgtgag cgccgccacg acaccgccgg ccagctcgcg gcgcacggcc
     9841 accaggggcg ccgcgcgcga gggaacgtgc tggtacggcg taccttgaca cctgtcaacc
     9901 tcaccacgga gcccgcatgc acgagcaccc cgccgcaagg accgcactcg ccgccatgca
     9961 ggcggtgacc cgcggcgatc gcgaggcctg gctcgcctgc taccgcgacg acgcggtcct
    10021 gcacgatccc gtcggcggtt cgccgctgga cccgggcggc gcggggctgc gcggcacgcg
    10081 ggcgctgcag gacttctggg atctgaccat cgcgccgaac gacgtggcct tcaccgttgc
    10141 ggccgtgcac ccggcggggg acgaggcggc cgtggtggcc tccgtgacca tccggttccc
    10201 gggcggcaac gccgtgagct acgacggggt cttcgtgtac cgcgtcgacg aggcggagcg
    10261 ggtcgcgtcg gtgcgcgcgt actgggacct tcagcgggtc ctgtcggccc tcggcgcctg
    10321 acccgccagc tcctcgacga aggccacgaa ctcggggggc ccaggcagtt cgtcgacccg
    10381 ccggcggaag tccgcgacgc ggccggccac cgcggggtcg gccgcgagcg cgcgcgcctc
    10441 ggtcagcacc tgttgcggat cgagccgtgc cgggtccgtc agcggcacgc cgaacgcccc
    10501 ctggccgagg gcgtggcgca gcaccgagcc gagcccgtgg cgcgtcacgg cgtcggcgtg
    10561 gctgccctgg tcgcccgtga gcggcaccac gagctgcggc gccccggccg acaggcactc
    10621 catcgtcgag ctgaagccgc cgtggtggac cgccagcgca cagcgcgaca ggatctcccg
    10681 ctgcggcgcg aacggcagca gccggacgtt gggcggctgc gggccgca
//
LOCUS       DATMKC010000370         9232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_29340, whole genome shotgun sequence.
ACCESSION   DATMKC010000370 DATMKC010000000
VERSION     DATMKC010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29340"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="VNU01_10890"
     CDS             <1..1998
                     /locus_tag="VNU01_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWH33163.1"
                     /translation="VGLLATGAGGYQPAYFPLVGLVAPPRVRSQAYAWAILFVGTGGL
                     MSILLFSLAETSGYRTAMTLLAFITAVASLTAMSAARFVRRDIAQAEQALGAAQRLRE
                     EFAATGSRALLVCRGVEVAYDQVQVLFGVDLEVQPGECVALLGTNGAGKSTLLRAISG
                     THDPSGGVIFFDGRDITHADANQTAAAGIAQVPGGKAVFPTLTVAEHLRAAGWLYRAE
                     KDYLARATAEVFQTFPRLKEREHQLAGDLSGGEQQMLGLGMAFIAKPKLLMIDELSLG
                     LAPAIVEQLLKILRRIQAQGTAIVLVEQSVNVALTVADRAYFLEKGEVRFEGPTADLL
                     ERPDVLRSVFLEGAARGRAAGAGEGAARGRAAGAGEGAATRGVSTPSIQDGLSRHTPG
                     APLLDVYGLTKRFGGIRAVDDATFRLHEGEILGLIGPNGAGKTTIFDLVSGFLVPDGG
                     RIVVGGQDITGMAADRRAWLGLGRSFQDARLVPSLTVAENLALGLERHLPIRDHVAAA
                     LGLPMVLRQEEDVAWTVADLVELMGLEAYRDKFVRELSTGTRRIVDLAMCIAHEPRVL
                     LLDEPSSGIAQRETEALGPLLRRIQAETGCALLVIEHDMPLITSISDRMIALELGHPI
                     AEGTPAEVVRDARVVASYLGGGGAATQRSGPAALAGVGEEE"
     gene            1998..2987
                     /locus_tag="VNU01_10895"
     CDS             1998..2987
                     /locus_tag="VNU01_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33164.1"
                     /translation="MAATRRVLARVLALWVGLAVAAAPVPAAALAPSQTGWWWRAQAG
                     LLVDVPHPPQVPEGGLYVEGAPDGPTAIAALRFRVPEDSVGIAVTLRVADEQGGDSAV
                     LLLCPAGSPWVPAQGGRWDARPAAACEGQGVLGTRAEDGSAWSFPLELLSFTGARDLV
                     LVPGVDDSRPEGVNGSVFAIAFEPPGESALITEDADMRDDPPSGPPRPPVFVPPTDTG
                     GAPPPFGGLAQEPPPAGEAAPELAPQSGDPSVIAPRVSTPPEAVPGRPVPAGKDARAL
                     AALVALLSVGVAGVLSRDRLAVVPGIPAAAAGDPREGGIGRFRRPRSSPPPPL"
     gene            3017..4546
                     /locus_tag="VNU01_10900"
     CDS             3017..4546
                     /locus_tag="VNU01_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH33165.1"
                     /translation="MTVGAHNLARLTEQAFERHGDYESTYFEGAWLRSGDLFERSRRL
                     AGGFVELGIAPGDRVVVMMANSPEVGLCYTALWRAGAVITPAIFLLPPDELRHVLENS
                     EARAIVTTPEFLGTVKAAAEGVETLKFILVLGHSEDGVVALESLESADPIGIVSRSDD
                     DLAALMYTGGTTGRAKGVMLTHENLWYCGKASEEASYVPGTRRTVVPLPLAHSFGLIV
                     TIVGAHGREPGQSALMRWFDPVGLLTLIQDLKMERATLVPSMIQILLTQPLEDYDLSS
                     WRFVSCGASPLSVEVAQEFERRVPTVTITEGYGCTESGAVISVNPPLARRLGSVGKPL
                     PGYEVRLDAGDGGPDAEGQPDPARLGEVVVRAPGVMRGYWKAPEVTAETLRDGWLHTG
                     DIGRFDEDGYLYIVDRKKDLIIRGGFNVFPRDVEDAMVEHPAVALCGVVGRPDPTYGE
                     EVVAFVQLMPGAQADEAELVAFAKQRLGGYKYPREVHILEGVPLTPVGKIDRKRLRTL
                     L"
     gene            4633..7158
                     /locus_tag="VNU01_10905"
     CDS             4633..7158
                     /locus_tag="VNU01_10905"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWH33166.1"
                     /translation="MLEALGDAVVVADARNTIVWANRAAAELLDWPDHDLVGQPLTML
                     MPERFRPAHVAAFERYVATRKATIMGRPVRVAALRRDGSEVPIELVLNPLPADSNALV
                     AASLRSMEDRIELELRSVVVSRLLAVLAEGGALTDVAPRVLEAIADTLGWQYAGLWVV
                     DWNDELVRLAASWSAPDVSLGGFTAVSESLSFRVGEGVPGRVVSDGAAVWIPDIAEEP
                     NFPRRDPAMADGLRSAFAFPVRAGRRMLGVIELLTTVRREPDDQLLEAMGAIGEHLGR
                     FVEQRLAEEAVQASRQRLELALEAGRLGTWEWDLRADVLHWYDSVEALVGPSGPHGRA
                     FDEAMELVHPEDRERVEACFREAAAGSGDLRCTYRVVAPDGRTRWFETSGRLMRDAAG
                     RTLLAGVAADVTDRMEAQQQREELLRDVQAAQEHLGLLAEAGELLSSSLDYGETLRHV
                     AGLSVPRLADVCLVHGVRDNGTLQLLAVEGAEEAARVVLRLHTEHPGAVRSLFDLGEV
                     ITRHEPVLHQEVTPAVLRRLAANGPHLAVLEALGWHSVLVVPLQARGRTLGAMTWAMT
                     DSGRAYGPRDVQFASQIAARAALAVDNARLYEDQANTARTLQESLLPPHLPAIPGIEV
                     SAGYRPAGRNQVAGDFYDLFPVRGDSWGIVMGDVRGKGAAAAAVTALARWTVRAAAMQ
                     SSSPPRVLALLNDAMVRRLEEGEDPRFCTAVFARLRAAADGAQLTVSSGGHPLPLMLR
                     RSGVVEELGEAGLIVGAFPEGDWQAARAQMGPGDVVLLYTDGVTEARVDGGELFGDAR
                     LRALLEECAGLTAGEVVQRVLKRLDEDAEPRDDIALLALRVRE"
     gene            complement(7174..7884)
                     /locus_tag="VNU01_10910"
     CDS             complement(7174..7884)
                     /locus_tag="VNU01_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HWH33167.1"
                     /translation="MSDRERALHDALATFVRRMTQDYDVGDVLDLLAGQVPAVMGVEA
                     GGVTLVDPKGTTAYAAASDPRAQRTEQLETTLDQGPCIACIADREIKVVPDLLRETRW
                     PAYGEAARQFGWRTVLGVPLMAGDRCIGGLDIYGEQPRDWTREELEHARLLADMATSY
                     IVHARALEESRTLAAQLQNALHSRVVIEQAKGMLAATLDLDVDQAFELLRTRARSTNV
                     TLRSVAQRVVEGRGAPQH"
     gene            7977..8049
                     /locus_tag="VNU01_10915"
     tRNA            7977..8049
                     /locus_tag="VNU01_10915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8010..8012,aa:Thr,seq:cgt)
     gene            8213..9115
                     /locus_tag="VNU01_10920"
     CDS             8213..9115
                     /locus_tag="VNU01_10920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWH33168.1"
                     /translation="MRLSVGLTNFGNHLEGDQWVPALRRLEDAGMHGVWLTDHVAVPH
                     HIESRYPFTPDGTFRLREADVWYDALVTLSYVAACTSTLELGIGVCVVGLRHPIILAK
                     QIATIDRLSGGRVALGIGSGWLREEFQVLDIDPSRPVARMEGALRLMKACWSGAPEPG
                     EYGPYKVDRPFHSLPTPAHEIPVVVGGEGPAALRASVEAADGWLGATFDLSQASVAHI
                     AEVAGRVRGLSNPGAVPPRVMTRVPVRRSDLDGALAHALRTWSEAGVDELVLDVNWAN
                     ADRSVTVLEQVVRQGRDLGLVDAA"
BASE COUNT         1138 a         3254 c         3551 g         1289 t
ORIGIN      
        1 gtgggcctgc tggccaccgg cgcgggcggc taccagccgg cgtacttccc gctggtcggg
       61 ctggtcgcgc cgccgcgggt gcgctcgcag gcctacgcct gggcgatcct gttcgtcggc
      121 accggcggcc tgatgtcgat cctgctgttc tcgctggcgg agacgtccgg gtaccgcacc
      181 gccatgacgc tgctggcgtt catcacggcg gtggccagcc tcacggcgat gagcgccgcc
      241 cgcttcgtgc gccgcgacat cgcgcaggcc gagcaggcgc tgggcgcggc gcaacggctg
      301 cgcgaggagt tcgccgcgac cggctcgcgt gcgctgctgg tgtgccgcgg cgtggaggtg
      361 gcctacgacc aggtccaggt gctgttcggc gtggacctgg aggtccagcc gggcgaatgc
      421 gtggcgctgc tgggcaccaa cggcgcgggc aagtccacgc tgctgcgcgc catcagcggc
      481 acgcacgacc cctccggcgg cgtgatcttc ttcgacggcc gcgacatcac ccacgccgac
      541 gcgaaccaga ccgccgcggc cggcatcgcc caggtgcccg gcggcaaggc ggtgttcccc
      601 acgctcaccg tcgccgagca cctgcgcgcc gccggatggc tgtaccgcgc cgaaaaggac
      661 tacctcgccc gggcgaccgc ggaggtcttc cagaccttcc cccgcctcaa ggagcgcgag
      721 caccagctcg cgggcgacct gtccggcggc gagcagcaga tgctgggact gggcatggcg
      781 ttcatcgcca agccgaagct gctcatgatc gacgagctgt cgctggggct ggcgccggcg
      841 atcgtcgagc agctgctgaa gatcctgcgg cgcatccagg cgcagggcac cgcgatcgtg
      901 ctggtggagc agtcggtcaa cgtggcgctg accgtggccg accgcgccta cttcctggag
      961 aagggggagg tgcgcttcga agggcccaca gccgacctgc tcgagcgccc cgacgtgctg
     1021 cgcagcgtgt tcctggaagg tgccgcgcgc ggccgtgcgg ccggcgctgg ggaaggcgcc
     1081 gcgcgcggcc gtgcggccgg cgctggggaa ggtgccgcga cccggggtgt gtcgactccg
     1141 tcaatccagg atggactcag tcgacacacc ccgggggcgc cgctgctgga cgtgtacggg
     1201 ctcaccaagc gcttcggcgg catccgcgcg gtcgacgacg ccacgttccg gctgcacgag
     1261 ggcgagatcc tgggcctcat cgggcccaac ggcgccggca agaccaccat cttcgacctg
     1321 gtgtccggct tcctggtccc cgacggcggg cgcatcgtgg tgggcggcca ggacatcacc
     1381 ggcatggcgg cggaccgccg ggcgtggctg ggcctgggcc gctcgttcca ggacgcccgc
     1441 ctggtgccat ccctgaccgt cgcggagaac ctcgcgctgg gactcgaacg gcacctgccg
     1501 atccgcgacc acgtcgcggc cgccctgggc ctgcccatgg tgctgcgcca ggaggaggac
     1561 gtcgcgtgga cggtcgcgga cctggtggag ctcatgggcc tggaggccta tcgggacaag
     1621 ttcgtgcgtg agctgtccac cggcacgcgg cgcatcgtcg acctggcgat gtgcatcgcg
     1681 cacgagccgc gtgtgctgct gctggacgag ccgtcctcgg gcatcgccca gcgcgagacg
     1741 gaggcgctcg ggccgctgct gcggcggatc caggcagaga ccggttgcgc cctgctggtc
     1801 atcgagcacg acatgccgct gatcacctcg atctccgacc ggatgatcgc gttggagctc
     1861 ggccacccca tcgccgaggg cacgcccgcc gaggtggtgc gcgacgcgcg cgtggtggcg
     1921 agctacctgg gcggcggcgg cgcggcgacc cagcgctcgg gcccggcggc gctcgccggc
     1981 gtgggggagg aggagtgatg gcggcgacca ggcgggtgct ggcacgggtc ctcgcgctgt
     2041 gggtgggcct cgccgtggcg gccgcgccgg tcccggcggc cgcgctcgcg cccagccaga
     2101 ccggatggtg gtggcgcgcg caggcggggc tgctggtcga cgtgccccat ccgccccagg
     2161 tgccggaggg cggcctgtac gtggagggcg cgccggacgg tcccacggcc atcgccgcgc
     2221 tgcgcttccg cgtccccgag gacagcgtcg gcatcgccgt gacgctgcgg gtcgccgacg
     2281 agcagggcgg cgacagcgcc gtgctcctgc tctgccccgc cggcagcccg tgggtccccg
     2341 cccagggcgg ccgctgggac gcccggccgg cggcggcgtg cgaaggccaa ggcgtgctgg
     2401 gcacccgggc cgaggacggc tccgcctggt cgttccccct ggagctgctg tcgttcaccg
     2461 gcgcgcgcga cctcgtgctg gtcccgggcg tcgacgactc gcgaccggag ggggtcaacg
     2521 gctcggtgtt cgcgatcgcc ttcgagccgc ccggggagtc ggcgctgatc accgaggacg
     2581 ccgacatgcg cgacgacccg ccatccgggc cgccgcgccc gcccgtgttc gtgccgccga
     2641 ccgacaccgg cggcgcgcca ccgcccttcg gcggcctggc gcaggagcca ccgcccgcgg
     2701 gcgaggccgc tccggagctg gcgccgcagt cgggcgaccc cagcgtcatc gcgccgcgcg
     2761 tgtccacgcc ccccgaggcg gtgccggggc gccccgtgcc ggccggcaag gacgcgcgcg
     2821 cgctcgcggc cctggtcgcg ctgctgtcgg tcggtgtcgc cggcgtgctg tcccgcgacc
     2881 gcctggcggt ggtgcccgga attccggcgg cggcggcggg agatccgcgc gagggcggca
     2941 tcggcaggtt ccgccggccg cgctccagcc cgccgccacc gctgtaggag cccgtgagag
     3001 cagaaagggt gctgacgtga ccgtgggagc gcacaacctc gcccgcctga ccgagcaggc
     3061 cttcgagcgc cacggcgact acgagtcgac ctacttcgag ggcgcctggc tgcgctcggg
     3121 ggacctgttc gaacggagcc ggaggctggc gggcggcttc gtcgaactcg gcatcgcccc
     3181 gggcgaccgg gtcgtcgtca tgatggcgaa cagcccggag gtggggctgt gctacacggc
     3241 gctgtggcgc gcgggggcgg tgatcacgcc ggcgatcttc ctgctgccgc ccgacgagct
     3301 gcgccacgtg ctggagaact ccgaggcccg cgcgatcgtc accacgccgg agttcctggg
     3361 cacggtgaag gccgcggcgg agggcgtgga gacgctcaag ttcatcctgg tgctggggca
     3421 ctccgaggac ggcgtggtcg ccctggagtc cctggagagc gccgatccca tcggcatcgt
     3481 gagccgctcc gacgacgacc tggccgcgct gatgtacacg ggcggcacga cggggcgggc
     3541 caagggcgtg atgctcacgc acgagaacct gtggtactgc ggcaaggcgt cggaggaggc
     3601 gagctacgtc cccggcacgc gccgcaccgt ggtgccgctg ccgctggcgc actccttcgg
     3661 actgatcgtc accatcgtgg gcgcgcacgg gcgcgagccg gggcagagcg cgctgatgcg
     3721 ctggttcgac ccggtcggcc tgctcacgct gatccaggac ctgaagatgg agcgggccac
     3781 gctggtgccc tccatgatcc agatcctgct gacgcagccg ctggaggact acgacctgtc
     3841 cagctggcgc ttcgtgtcct gcggcgcgtc gccgctgtcg gtggaggtcg cgcaggagtt
     3901 cgagcggcgc gtgcccaccg tcaccatcac cgagggctac ggctgcaccg agtccggcgc
     3961 ggtgatctcg gtgaacccgc ccctggcccg gcgtctgggc tcggtgggca agccgctgcc
     4021 gggctacgag gtgcgcctcg acgcgggcga cggtggcccg gacgccgagg ggcagcccga
     4081 cccggcgagg ctgggcgaag tcgtggtgcg cgcgccgggt gtcatgcgtg gctactggaa
     4141 ggcgccggag gtcacggccg agacgctgcg ggacggctgg ctgcacaccg gtgacatcgg
     4201 ccgcttcgac gaggacggct acctctacat cgtggaccgc aagaaggatc tgatcatccg
     4261 cggcggcttc aacgtgttcc cgcgcgacgt cgaggacgcc atggtggagc acccggccgt
     4321 ggcgctgtgc ggcgtggtgg ggcggccgga ccccacctac ggcgaggaag tggtcgcgtt
     4381 cgtgcagctc atgcccggcg cgcaggcgga cgaggcggag ctggtggcgt tcgccaagca
     4441 gcggctcggg gggtacaagt accccaggga ggtccacatc ctggaaggtg tgccgttgac
     4501 gcccgtgggg aagatcgacc ggaagcggct gcgcaccttg ctgtaggcgg gccgccccgc
     4561 gtccgcgccg tcccagaggg ggccgtcatc ccgtcgcgcc gtccaccgcg catctcgctg
     4621 gaggagcgag cgatcctcga ggcgctcggc gacgccgtcg tggtcgcgga cgcccgcaac
     4681 accatcgtgt gggccaaccg cgccgcggcc gagctgctgg actggccgga ccacgacctg
     4741 gtgggccagc cgctcaccat gctcatgccg gagcggttcc ggcccgccca cgtggcggcc
     4801 ttcgagcgct acgtcgcgac gcgcaaggcc accatcatgg gccgccccgt gcgcgtcgcc
     4861 gcgctgcgcc gcgatggcag cgaggtgccg atcgagctgg tgctgaaccc gctgccggct
     4921 gactccaacg cgctggtggc cgcttcactg cgcagcatgg aggaccgcat cgagctggag
     4981 ctgcgctccg tggtggtgag ccgcctgctc gcggtcctgg cggagggcgg cgcgctcacc
     5041 gacgtggcgc cgcgcgtgct ggaggccatc gccgacacgc tgggctggca gtacgccggc
     5101 ctgtgggtgg tggactggaa cgacgagctg gtgcgcctgg cggcgagctg gtcggcgccc
     5161 gacgtgtcgc tggggggctt caccgccgtc agcgagtcgc tgtcgttccg cgtgggggag
     5221 ggcgtgcccg gccgcgtggt gtccgacggc gcggcggtgt ggatccccga catcgccgag
     5281 gagcccaact tcccccgccg ggacccggcg atggccgacg ggctgcgctc ggcgttcgcg
     5341 ttccccgtgc gggcgggcag gcgcatgctc ggggtgatcg agctgctcac caccgtgcgg
     5401 cgggagcccg acgaccagct gctggaggcg atgggcgcca tcggcgagca cctcgggcgc
     5461 ttcgtggagc agcgcctggc cgaggaggcc gtgcaggcga gccggcagcg cctggagctg
     5521 gcgctggaag ccgggcgcct cggcacgtgg gagtgggacc tgcgcgcgga cgtgctgcac
     5581 tggtacgaca gcgtggaggc gctggtgggc ccgtccggtc cgcacggcag ggccttcgac
     5641 gaggccatgg agctggtcca cccggaggac cgcgaacggg tcgaggcctg cttccgcgag
     5701 gcggccgccg gcagcggcga cctgcgctgc acctaccgcg tggtcgcccc cgacgggcgg
     5761 acgcgctggt tcgagacgtc gggacggctg atgcgcgacg ccgccggccg cacgctgctg
     5821 gccggcgtgg ccgcggacgt gaccgaccgc atggaggcgc agcagcagcg cgaggagctg
     5881 ctgcgcgacg tgcaggcggc ccaggagcac ctgggactgc tcgccgaggc cggtgagctg
     5941 ctgtcctcct cgctcgacta cggcgagacg ctgcgccacg tggcgggact gtcggtcccg
     6001 cgcctggccg acgtgtgcct ggtgcacggc gtgcgggaca acggcacgct gcagctcttg
     6061 gccgtcgagg gcgccgagga ggccgcgcgc gttgtcctgc gcctgcacac cgagcatccc
     6121 ggcgcggtgc ggtcgctgtt cgacctgggc gaggtgatca cccggcatga gcccgtgctg
     6181 caccaggagg tgacgccggc ggtcctgcgg cggctggccg ccaacgggcc gcacctggcc
     6241 gtgctcgagg cgctcggctg gcacagcgtg ctggtggtgc cgctgcaggc gcgcgggcgc
     6301 accctcggcg cgatgacctg ggcgatgacc gactcgggcc gcgcctacgg cccgcgtgac
     6361 gtccagttcg cctcgcagat cgccgcgcgc gcggcgctgg ccgtggacaa cgcgcgcctg
     6421 tacgaggacc aggccaacac ggcgcgcacg ctccaggaga gcctgctgcc gccgcatctg
     6481 cccgcgatcc ccggcatcga ggtctcggcc ggctaccgcc ccgccggccg caaccaggtg
     6541 gcgggggact tctacgacct cttcccggtg cgtggcgact cgtggggcat cgtcatgggg
     6601 gacgtgcgcg gcaagggcgc ggccgcggcg gcggtgacgg cgctggcgcg ctggaccgtg
     6661 cgcgcggccg ccatgcagtc atccagcccg ccgcgcgtgc tggccctgct caacgacgcg
     6721 atggtgcgcc ggctggagga aggcgaggac ccgcgcttct gcaccgccgt gttcgcgcgc
     6781 ctgcgcgcgg ccgcggacgg cgcccagctc accgtgtcca gcggcggcca cccgctgccg
     6841 ctgatgctgc ggcgcagcgg cgtggtggag gagctcgggg aagccgggct catcgtcggc
     6901 gcgttcccgg agggcgactg gcaggcggcc cgcgcgcaga tgggaccggg cgacgtggtc
     6961 ctgctctaca ccgacggcgt cacggaggcg cgcgtggacg gtggtgagct gttcggtgac
     7021 gcgcgcctgc gcgcgctgct ggaggagtgc gcgggcctga ccgccggcga ggtcgtccag
     7081 cgcgtcctga agcgcctcga cgaggacgcc gagccgcgcg acgacatcgc gctgctggcg
     7141 ctgcgcgtgc gcgagtaggc ggcgagggcc gcctcagtgc tgcggcgcgc cccggccctc
     7201 gaccacccgc tgcgccacgc tgcgcagcgt gacgttggtg gagcgggcgc gcgtgcgcag
     7261 cagctcgaac gcctggtcca cgtccaggtc gagcgtggcg gccagcatgc ccttggcctg
     7321 ttcgatgacc acgcggctgt gcagcgcgtt ctggagctgg gccgccagcg tgcgcgactc
     7381 ctccagcgcg cgggcgtgga cgatgtagct cgtggccatg tcggccagga gccgcgcgtg
     7441 ttccagctcc tcccgcgtcc agtcgcgcgg ctgctctccg tagatgtcca ggccgccgat
     7501 gcagcggtcg ccggccatga gcggcacgcc cagcaccgtg cgccagccga actgccgcgc
     7561 cgcctcgccg taggccggcc agcgcgtctc gcgcagcagg tcgggcacca ccttgatctc
     7621 gcgatcggcg atgcaggcga tgcagggacc ctggtccagc gtggtctcga gctgttcggt
     7681 ccgctgggcg cgggggtcgc tcgccgcggc gtaggcggtg gtgcccttcg ggtcgaccag
     7741 cgtcacgccc cccgcctcca cgcccatgac cgcgggcacc tggccggcca gcaggtccag
     7801 cacgtcgccc acgtcgtagt cctgggtcat ccgccggacg aaggtggcca gggcgtcatg
     7861 gagggcgcgc tcgcggtcgc tcatgggggc aactatggcc gccggcctct ttgcgaaacc
     7921 tggccgactt gccgaggggc gcgcaggcgc tacccttctc ccgcgcgttc gcgcacgccg
     7981 ggatagctca gtcggcagag cgattcactc gtaatgaata ggtccggggt tcgattcccc
     8041 gtcccggctc cgcgtcggag accccgcttc ggcggggtct ccgacctttg ggagcccatc
     8101 cccgcgagcc cgttccttgc ggcggacatc gccggccgtt gacggtcgtc cgccgcgatg
     8161 gtattctatc gatcgaacga cagatgaccg gccaccgacg ttggaacagt gcatgcggct
     8221 tagcgtgggc ttgacgaact tcggcaacca cctcgagggc gatcagtggg tgcctgcgct
     8281 gcggcgtctg gaggacgccg gcatgcatgg tgtctggctc actgaccacg tcgccgtccc
     8341 tcaccacatc gagtcgcgct accctttcac ccccgatggc acgttccgtc tgcgcgaggc
     8401 cgatgtgtgg tacgacgcgc tggtgacgct gtcctacgta gcggcgtgca cgagcaccct
     8461 ggagcttggc atcggcgtgt gcgtggtggg ccttcgccac cccataatct tggccaagca
     8521 gatcgcgacc atcgaccgcc tttccggcgg aagggtggcg ctcggtatcg ggagcggctg
     8581 gctgcgcgag gagtttcagg tgctcgacat cgatcccagc cggccggtgg cccgaatgga
     8641 gggtgcgctg cgcctgatga aggcctgctg gtcgggtgcg cccgagcccg gcgagtacgg
     8701 cccctacaag gtggaccgcc cgttccacag cctgcccacc cccgcacacg agatcccggt
     8761 ggtggtgggc ggcgagggcc ccgctgccct cagggccagc gtggaggccg ccgacggctg
     8821 gctgggggcg acgttcgatc tgtcacaggc gtcggtcgcc cacatcgccg aggtcgccgg
     8881 gcgcgtgcgg ggcctttcca acccgggggc cgtaccacct cgggtgatga cgcgggtgcc
     8941 cgtgcggcgg agcgacctgg acggcgcgct cgcccacgcc ctcaggactt ggagcgaggc
     9001 cggtgtggac gaactcgtcc tggacgtgaa ctgggcgaat gccgatcggt ccgtgaccgt
     9061 gctggagcag gtggtgcggc agggccgcga cctcggcctg gtcgatgcgg cgtgagcggc
     9121 caggccacgg cgccgctggc gggcgagggg cgggtcatcg tcaccggtgc cgggcgggga
     9181 ctgggccgag agatcgcact cgtgctggcc gcggccgggg cgcgtgtcgc ca
//
LOCUS       DATMKC010000371         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_184136, whole genome shotgun sequence.
ACCESSION   DATMKC010000371 DATMKC010000000
VERSION     DATMKC010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184136"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1369
                     /gene="pdhA"
                     /locus_tag="VNU01_10925"
     CDS             242..1369
                     /gene="pdhA"
                     /locus_tag="VNU01_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWH33169.1"
                     /translation="MTRADEPLVPDPVSLLPPHPPVQLLDDHGELHEVEGFPLDLKEE
                     DFRALYRHMVLARRVDRQAINLQRQGQLGVYASLLGQEAAQVGGAYALAEQDWVFPSY
                     REMGAALVRGLDPSAILHLFRGTWLSDHDPHRHRFGLLSIPIGTQALHAAGFAMGAKF
                     DGKDLVVLAYFGDGATSEGDPHEAMNFAAVFDAPVVFFVQNNQYAISVPLSKQTHAPT
                     LAHKAVGYGMPGYRCDGNDVLASYAVTKQAVDRARAGQGPSMVEAVTYRMEAHTTADD
                     ATRYRTPEELEEWRRRDPIARFEHFLEREGLLDAGLKQEVEREGEELARRMREEIADA
                     PHGDPLELFAHVYADPPATYAEQRELLRRELAAGDDAEGEA"
     gene            1370..>2100
                     /locus_tag="VNU01_10930"
     CDS             1370..>2100
                     /locus_tag="VNU01_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWH33170.1"
                     /translation="MAVTLAQALNRALHDAMEADDRVVMFGEDVGRLGGVFRITDKLQ
                     ERFGERRCFDTPLAESGIVGTAIGMAMYGWKPVVEMQFDGFTYPAFEQIVSHLAKMRN
                     RSRGTVDLPVVIRIPFGGGIGAVEHHSESPEAYFAHTAGLKVVSPGTPSDAYSLLREA
                     VESRDPVIFMEPKRRYWLKEDVELPVRTEPFGRAVVRRPGTTATLLAYGPMVRTCLDA
                     AEAAREEGWELEVVDIRSLVPFDEDT"
BASE COUNT          313 a          742 c          771 g          274 t
ORIGIN      
        1 tcctgccagc aggatcagcc ggtcgccgca cggccccgtg cgtgcgccgc ccgcgtgtga
       61 cgttccgtgg aaacggaaac ccttgccgtc acacgtcggg cccccgtacg ctcgcgcaag
      121 agccctgcaa agggacggtc aagcccgaga cccgggcctg ccgatccttt cagggagcgc
      181 ccgacacgcc ggaaacgccg gacgcggagg accccgtgag ccggtggatc gaggagtcga
      241 gatgacgcgc gccgatgagc cgctcgtgcc ggacccggtc tcgctgctgc cgccgcatcc
      301 gccggtgcag ctgctggacg accacgggga actgcacgag gtcgagggct ttcccctgga
      361 cctcaaggaa gaggacttcc gcgccctgta ccggcacatg gtgctggccc ggcgcgtcga
      421 ccggcaggcc atcaacctcc agcggcaggg ccagctcggc gtgtacgcgt cgctgctggg
      481 ccaggaggcc gcccaggtcg gcggcgccta cgcgctggcc gagcaggact gggtgttccc
      541 gtcctaccgc gagatgggcg ccgccctggt gcgcggcctg gaccccagcg cgatcctgca
      601 cctgttccgc ggcacgtggc tgtccgacca cgacccgcac cgccaccgct tcggcctgct
      661 gtccatcccc atcggcacgc aggcgctgca cgccgcgggc ttcgcgatgg gcgcgaagtt
      721 cgacggcaag gacctggtgg tgctggccta cttcggcgac ggggcgacca gcgagggcga
      781 cccgcacgag gccatgaact tcgccgccgt cttcgacgcg ccggtggtgt tcttcgtgca
      841 gaacaaccag tacgcgatca gcgtgccgct gagcaagcag acgcacgcgc ccacactggc
      901 gcacaaggcc gtcggctacg gcatgccggg ctaccgctgc gacggcaacg acgtgctggc
      961 ctcctacgcg gtcaccaagc aggcggtgga ccgggcccgc gccggccagg gcccgtcgat
     1021 ggtggaggcg gtcacctacc gcatggaggc gcacaccacc gccgacgacg cgacgcgcta
     1081 ccgcacgccg gaggagttgg aggagtggcg ccggcgcgac cccatcgcgc gcttcgagca
     1141 cttcctggag cgcgaggggc tgctggacgc cgggctcaag caggaggtcg agcgcgaggg
     1201 cgaggagctg gcccggcgga tgcgcgagga gatcgccgac gcgccgcacg gcgacccgct
     1261 ggagctgttc gcgcacgtct acgccgaccc gccggccacc tacgccgagc agcgcgagct
     1321 gctgcggcgc gagctggcag ccggcgacga cgccgagggg gaggcgtaga tggcggtcac
     1381 gctggcgcag gcgctgaacc gcgcgctgca cgacgcgatg gaagccgacg accgcgtggt
     1441 catgttcggc gaggatgtcg ggcggctggg cggcgtgttc cgcatcaccg acaagctgca
     1501 ggagcgcttc ggcgagcggc gctgcttcga cacgccgctg gccgagtccg gcatcgtggg
     1561 gaccgcgatc ggcatggcga tgtacggctg gaagcccgtg gtggagatgc agttcgacgg
     1621 cttcacctac ccggccttcg agcagatcgt cagccacctc gccaagatgc ggaaccgctc
     1681 gcggggcacg gtcgacctgc ccgtggtgat ccgcatcccg ttcggcggcg gcatcggcgc
     1741 ggtggagcac cactcggaat cgcccgaggc ctacttcgcg cacaccgccg ggctgaaggt
     1801 ggtctcgccg ggcacgccgt cggacgcgta ctcgctgctg cgcgaggcgg tggagtcccg
     1861 cgaccccgtc atcttcatgg agccgaagcg gcgctactgg ctcaaggagg acgtcgagct
     1921 gccggtgcgc acggagccgt tcggccgtgc cgtggtccgc cgcccgggga cgacggcgac
     1981 gctgctggcc tacggcccga tggtgcgcac ctgcctggac gcggccgagg ccgcccgcga
     2041 ggagggctgg gagctcgagg tcgtggacat ccgctcgctg gtgcccttcg acgaggacac
//
LOCUS       DATMKC010000372         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_61753, whole genome shotgun sequence.
ACCESSION   DATMKC010000372 DATMKC010000000
VERSION     DATMKC010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61753"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VNU01_10935"
     CDS             <1..321
                     /locus_tag="VNU01_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33171.1"
                     /translation="GGVAAALAPTVLRLSGEGGSPGAVTASTAAGAALLLGLAHLPRM
                     RGVEALLLRVLALPVWLGGLALLMAGSVLALPAALLAGRAGEVFLRLARALAGAVETF
                     LPPR"
     gene            405..1271
                     /locus_tag="VNU01_10940"
     CDS             405..1271
                     /locus_tag="VNU01_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HWH33172.1"
                     /translation="MAARIIDGKATAARVRERVAAEVAELAGKGVVPGLAAVLVGGDE
                     ASQVYVAGKERDCEAVGMRNFGAKLPGDVSQEELHAELDRLNADEQVSGIIVQLPLPS
                     HLDPIAAQERVSPGKDVDGLHPVSAGRLLRGDPLFVPATPLGVQVLLSEYEIPVAGAD
                     VVIVGRSQLVGRPLALLLTLKGQGANATVTMCHTGTRDLSAHTTRADIVVVATGVPHT
                     LTADMVKPGATVIDVGINRDEDGKLFGDVDYEEVSRVAGAITPVPGGVGPMTRAMLLH
                     NTLLAAKAQHGL"
     gene            complement(1268..1822)
                     /locus_tag="VNU01_10945"
     CDS             complement(1268..1822)
                     /locus_tag="VNU01_10945"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWH33173.1"
                     /translation="MAADAAARWVAFLRAINVGGRRVRMDALRDLFGELGFADVATHV
                     ASGNVIFRAAGTSEQMRARIEAGLRDALGFDVPAYLRSERDVAGIVAAHPFGDVPEAQ
                     VQVAFLRTPPTAAALRALRALQTPRDRLEPGGAHLYWLATDGVGRSTLSWPKVEAALG
                     SPTTVRSLTSLRRLAAANGMGASG"
     gene            complement(1812..>2737)
                     /locus_tag="VNU01_10950"
     CDS             complement(1812..>2737)
                     /locus_tag="VNU01_10950"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWH33174.1"
                     /translation="VEELADSAGISIPMLRAWESAIGLPSHERYRPSDAEDARLLARL
                     VAYLPGSEVDALVRPFRADGQALQRIAMAHLELVYRNFIQPLRAAGGNDVAVAIALAE
                     AAHALLPLAGPLVGTAYRRIMEHLLSTELVAQATRGAVDRMDLAVAFVDVVGYTSLSA
                     RIDPEGLDEVIDAFETRCYTVAGLNERIQLVKFLGDAAMYISTDAVALARAMLDVTEY
                     AEHDSPLAGAPMRAGLAFGECLVRQGDYFGPPVNLAARLTDRARAGTVLAQPDLAETL
                     EGHFALRRLPPIHLRGLGHLRPVAVRRTDGG"
BASE COUNT          344 a          985 c         1072 g          336 t
ORIGIN      
        1 ggcggcgtcg ccgctgcgct cgcgcccacc gtcctgcgcc tgtccgggga gggcggctcg
       61 ccgggggccg tgacggcgtc gaccgccgcg ggcgccgcgc tcctgctggg gctggcccac
      121 ctgccgcgca tgcgcggcgt ggaggcactg ctgctgcgcg tgctcgcgct gccggtgtgg
      181 ctgggtgggc tggcgctgct catggcgggc agcgtcctcg cgctgccggc cgccctgctg
      241 gccggccgcg ccggcgaggt gttcctgcgc ctggcgcgcg cgctggccgg cgcggtggag
      301 acgttcctgc cgccacgctg accggggccg tgccatggac gccttcggcc cacgcgccgc
      361 gctttggcat gatgcccgca ccggtgtgca cgacaggaga cctcatggca gcgcgcatca
      421 tcgacggcaa ggcgaccgcg gcacgcgttc gcgagcgtgt ggcggcggag gtcgccgagc
      481 tcgcgggcaa gggggtcgtc cccgggctgg cggcggtgct cgtgggtggc gacgaggcca
      541 gccaggtgta cgtcgcgggc aaggagcgcg actgcgaggc ggtcggcatg cgcaacttcg
      601 gcgcgaagct gcccggtgac gtcagccagg aggagttgca cgccgagctg gaccggctca
      661 acgccgacga gcaggtgagc gggatcatcg tgcagctgcc gctgccgtcg cacctggacc
      721 cgatcgcggc gcaggagcgc gtgtcgcccg gcaaggacgt cgacggcctg cacccggtca
      781 gcgccgggcg gctgctgcgc ggcgacccgc tgttcgtgcc ggcgacgccg ctgggcgtgc
      841 aggtgctgct cagcgagtac gagatccccg tggcgggcgc cgacgtggtg atcgtcggcc
      901 gctcgcagct ggtgggccgg ccgctggcgc tgctgctcac gctgaagggc cagggcgcca
      961 acgcgaccgt gacgatgtgc cacaccggca cgcgcgacct gtccgcgcac accacgcgcg
     1021 ccgacatcgt ggtggtggcc accggcgtgc cgcacacgct caccgccgac atggtgaagc
     1081 cgggcgcgac cgtgatcgac gtgggcatca accgcgacga ggacggcaag ctgttcggcg
     1141 acgtcgacta cgaggaggtc tcccgggtcg cgggcgccat cactccggtg cccggcggtg
     1201 tgggcccgat gacgcgggcg atgctgctgc acaacacgct gctggccgcc aaggcgcagc
     1261 acggcctcta gccggacgcg cccatgccgt tggcggccgc cagccgccgc agcgacgtga
     1321 ggctgcgcac ggtggtcggc gagccgagcg ccgcttccac cttcggccac gacagcgtgg
     1381 agcggcccac gccgtcggtg gccagccagt acaggtgcgc gccgccgggc tccagccggt
     1441 cgcgcggcgt ctgaagcgcc cgcagcgcgc gcagtgccgc ggccgtgggc ggcgtacgca
     1501 ggaacgcgac ctgcacctgc gcctcgggga cgtcaccgaa cgggtgggcc gccacgatgc
     1561 cggccacgtc gcgctcgctg cgcaggtagg cgggcacgtc gaagcccagg gcgtcgcgca
     1621 gccccgcctc gatgcgtgcg cgcatctgct cgctcgtgcc ggcggcgcgg aagatcacgt
     1681 tgccgctggc gacatgcgtg gcgacgtcgg cgaagccgag ctcgccgaac aggtcacgca
     1741 gggcgtccat ccgcacgcgg cggccgccga cgttgatcgc gcgcaggaac gcgacccagc
     1801 gcgcggccgc gtcagccgcc atcggtccgg cgcacggcga cgggccgcag atggcccagg
     1861 ccgcgcaggt ggatgggcgg caggcggcgg agggcgaagt gcccttccag ggtctcggcg
     1921 aggtccggct gggccagcac ggtgccggcg cgggcgcggt cggtgaggcg ggcggccagg
     1981 ttcacgggcg ggccgaagta gtcgccctgg cgcaccaggc actcgccgaa cgccagcccg
     2041 gcgcgcatcg gggcgccggc cagcgggctg tcgtgctcgg cgtactcggt gacgtcgagc
     2101 atcgcgcggg ccagcgcgac cgcgtccgtg gagatgtaca tcgcggcgtc gcccaggaac
     2161 ttcaccagct ggatgcgctc gttcagcccg gcgaccgtgt agcagcgcgt ctcgaacgcg
     2221 tcgatgacct cgtccaggcc ctccgggtcg atgcgcgccg acagcgacgt gtacccgacc
     2281 acgtccacga acgcgaccgc gaggtccatg cggtccacgg cgccgcgggt ggcctgggcg
     2341 accagttcgg tggacagcag gtgctccatg atgcggcggt aggccgtgcc gaccagcggg
     2401 cccgccagcg gcagcagcgc gtgcgccgcc tcggccagcg cgatcgccac cgcgacgtcg
     2461 ttgcccccgg ccgcgcgcag cggctggatg aagttgcggt agaccagctc caggtgcgcc
     2521 atcgcgatgc gctgcagcgc ctggccgtcg gcgcggaacg ggcgcaccag cgcgtcgacc
     2581 tccgagccgg gcaggtaggc gaccagccgg gcgagcaggc gcgcgtcctc ggcgtcgctg
     2641 ggccggtagc gctcgtgcga cggcagccct atcgcggact cccatgcgcg cagcatcggg
     2701 atggagatcc cggcggagtc ggcgagctcc tccacgg
//
LOCUS       DATMKC010000373         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_176311, whole genome shotgun sequence.
ACCESSION   DATMKC010000373 DATMKC010000000
VERSION     DATMKC010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176311"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VNU01_10955"
     CDS             <1..482
                     /locus_tag="VNU01_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWH33175.1"
                     /translation="DKLGKGFYLQMDAFANGRLQTAARALGVMQAAFEDAVNYANERH
                     VFGVALGDYQLTRSKLARMAMLIAACRRFSYRSAQLLGTGDGQLEASMVKAFSCLAAE
                     WVTREAQQIHGGYGYAEEYAVSRYFVDARVLSIFEGADETLALRVIVRRLLEDALAS"
     gene            complement(543..1607)
                     /gene="rsgA"
                     /locus_tag="VNU01_10960"
     CDS             complement(543..1607)
                     /gene="rsgA"
                     /locus_tag="VNU01_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HWH33176.1"
                     /translation="MTTDALPPDLIAYGWDAGWAEAFALATAGDAESVPARVARVDRG
                     ACTAAGEGRSYRVQLLPRTEWPVTGDWLAVRPSASERELDQVAAVLPRRKALRRMDAD
                     ERRSQLVAANVDVVLAVIPLDRPFKPGLRDRLLVLAAEAQAEPVLVLSKADIADPVQL
                     AVAESDAPPGFPQLVVSAARGEGVEAVRDAIGAGRTAVLLGPSGAGKSTLANALVGQD
                     VQRTGEVREGDRKGRHTTTARELMPLPGGGVLMDTPGVRALGLWDADEGIEEVFADIA
                     ELAQQCRFGDCAHDSEPGCAVRQAIEDGELDADRFARWRALLDEVEAQERRRVEHERR
                     AHERAFSRHVRDTLKRKGRK"
     gene            1686..>2134
                     /locus_tag="VNU01_10965"
     CDS             1686..>2134
                     /locus_tag="VNU01_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWH33177.1"
                     /translation="MAEIRETPLPGVGLRYDFRTDAGDLIGVIAHRSGRRELLVYDRE
                     DPDRCREALSLDEEDAWTLGEILGGPKLTGPLATLQQSIQGLTIDWYRVEEGHASAGS
                     TIGALQVRRRSGTTVVAVQRGDHTLPSPTPELELQLGDVAELQLQLRG"
BASE COUNT          286 a          793 c          777 g          278 t
ORIGIN      
        1 aggacaagct gggcaagggc ttctacctgc agatggacgc gttcgcgaac ggccgcctgc
       61 agacggcggc gcgcgcgctg ggcgtgatgc aggccgcgtt cgaggacgcc gtcaactacg
      121 ccaacgagcg ccacgtcttc ggcgtcgcgc tgggcgacta ccagctcacc aggtccaagc
      181 tcgcgcgcat ggcgatgctg atcgccgcgt gccgccgctt cagctaccgc tcggcgcagc
      241 tcctgggcac cggcgacggc cagctcgagg cgtcgatggt gaaggcgttc agctgcctgg
      301 cggcggagtg ggtcacgcgc gaggcgcagc agatccacgg cggctacggc tacgccgagg
      361 agtacgcggt cagccgctac ttcgtcgacg cgcgggtgct gtcgatcttc gagggcgccg
      421 acgagacgct ggccctgcgc gtcatcgtcc gccgactgct cgaggacgcc ctcgcctcct
      481 gattcccaac cgggaacgtt cgtccgagat accggacgaa cgttccacat tggcaaggcg
      541 ggctacttgc ggcctttgcg cttgagggtg tcgcgcacgt gtcgagagaa ggcgcgctcg
      601 tgcgcccgcc gctcgtgctc gacgcggcgg cgctcctggg cctccacctc gtcgagcagc
      661 gcgcgccagc gcgcgaagcg atccgcgtcc agttcgccgt cctcgatcgc ctggcgcacc
      721 gcgcacccgg gctcggagtc gtgcgcgcag tcgccgaagc ggcactgctg cgccagctcg
      781 gcgatgtcgg cgaacacttc ctcgatgccc tcgtcggcgt cccacaggcc cagcgcgcgc
      841 acccccgggg tgtccatgag cacgccgccg cccggcagcg gcatgagctc gcgcgccgtg
      901 gtggtgtggc ggcccttgcg gtcgccctcg cgcacctcac cggtgcgctg cacgtcctgg
      961 ccgaccagcg cgttggccag cgtggacttg cccgcgccgg acgggccgag cagcaccgcc
     1021 gtgcgtcccg ccccgatggc gtcgcgcacc gcttcgacgc cctcgccgcg ggcggcgctc
     1081 accacgagct gcgggaagcc cggaggcgca tccgactccg cgacggcgag ctgcaccggg
     1141 tcggcgatgt cggctttgct cagcacgagc accggttcgg cctgcgcctc ggcggccagc
     1201 accagcagcc ggtcgcgcag gccaggcttg aacggccggt ccagcgggat cacggcgagc
     1261 accacatcga cgttcgcggc cacgagctgc gagcgccgct cgtcggcgtc catgcggcgc
     1321 agcgccttgc gcctcggcag caccgcggcc acctggtcca gctcccgttc cgaggcgctg
     1381 ggccggacgg cgagccagtc gccggtgacc ggccactcgg tgcggggcag gagctgcacg
     1441 cggtaggagc ggccctcgcc cgccgcggtg cacgcgcccc ggtcgacgcg ggcgacgcgg
     1501 gccgggaccg actcggcatc gccggcggtc gcgagggcga acgcctcggc ccagccggcg
     1561 tcccagccgt aggcgatgag atcggggggc agcgcgtcgg tcgtcatggg tgcagcgcag
     1621 tgtcgcccac aggaacgggc cctgtcaggc cgccgcaccc gcacgctttc cggcagactg
     1681 gccgtatggc cgagatcaga gagaccccgc tgcccggtgt cgggctccgc tacgacttcc
     1741 gcacggacgc gggcgacctg atcggcgtga tcgcgcaccg gtcgggccgc cgcgagctgc
     1801 tcgtgtacga ccgggaggac cccgaccgct gccgggaggc gctgagcctc gacgaggagg
     1861 acgcctggac gttgggcgag atcctgggcg ggccgaagct gaccggtccg ctggccacgc
     1921 tccagcagag catccagggc ctgaccatcg actggtaccg cgtggaggaa ggccacgcca
     1981 gcgcgggcag caccatcggc gcgctgcagg tgcgccgccg cagcggcacc acggtggtcg
     2041 cggtgcagcg cggcgatcac accctgccct cgcccacccc ggagctggag ctgcagctcg
     2101 gcgacgtcgc cgagctgcag ctccagctcc gggg
//
LOCUS       DATMKC010000374        10898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_315344, whole genome shotgun sequence.
ACCESSION   DATMKC010000374 DATMKC010000000
VERSION     DATMKC010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315344"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="hisA"
                     /locus_tag="VNU01_10970"
     CDS             complement(<1..722)
                     /gene="hisA"
                     /locus_tag="VNU01_10970"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWH33178.1"
                     /translation="MSFTLYPAVDIKEGRAVRLVQGREDAETVYDDDPVQAAVRFAEQ
                     GAQWLHVVDLDAAFRGEPRNRHLIADIVKATGIPVQASGGIRSMADLRDSVDYGASRV
                     VIGTMALEEPSFVAAAVAEYGDKVAVGLDAEGTTLKARGWTTESGDLFDALRRFTMMG
                     VARFVFTDISRDGMLTGPNVERLREVAEATSAKVTASGGVSSLEDLRALASAHERVDG
                     AIVGKALYAGRFTLPEALALDAG"
     gene            complement(719..1351)
                     /gene="hisH"
                     /locus_tag="VNU01_10975"
     CDS             complement(719..1351)
                     /gene="hisH"
                     /locus_tag="VNU01_10975"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWH33179.1"
                     /translation="MSGPGPRVAVLDYEAGNLRSAEKALARAGADAFVTADAAEAAGA
                     DAIVVPGVGHFGQCARQFREAGFEQLVRDWSARGRPLLGICVGMQILYQGSEEDPATP
                     GLGLLPGRVRRLPGDVVVPHMGWNTVEAAREDPLVDGVDGKHAYFVHSYYADPADDAH
                     VVATADYGPGFPCVVRVGSVVGTQFHPEKSGDVGRRMLENFVDEVRGQVA"
     gene            complement(1348..1944)
                     /gene="hisB"
                     /locus_tag="VNU01_10980"
     CDS             complement(1348..1944)
                     /gene="hisB"
                     /locus_tag="VNU01_10980"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWH33180.1"
                     /translation="MSDAPRTGSVVRQTKETKVEVRVDLDGDGTCEISTGVPFFDHML
                     DQLGRHGRLNLTVRTDGDLHVDAHHTVEDTGIALGQALAEALGDKAGVERFGDATVPI
                     DEALVRVAVDLSGRPYLVYDADTPVELVGTYETTLTKHFFEALVANARCTLHVQKLAG
                     EGSHHIQEAVFKAVAVALRRAVRVTGSAIPSTKGTLGG"
     gene            complement(1941..3041)
                     /gene="hisC"
                     /locus_tag="VNU01_10985"
     CDS             complement(1941..3041)
                     /gene="hisC"
                     /locus_tag="VNU01_10985"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWH33181.1"
                     /translation="MSGDTPSRVPVRGDLADVVPYGAPQLDVPVRLNTNETPFAPPAE
                     YLKRLAERVETLPLHRYPDRRAWALRTALGERFGLPPERVWAANGSNELLLQLFQAYG
                     GPGRTLLLFRPGYSPYPLYAQIALTDSVQADLDGDYRLTTEIALAAVEAHDPELICVT
                     SPNNPTGMPVGLDVVRALHDASRALVIVDEAYVEFSQNHGDGAGGVSATTVLDELPRV
                     VVTRTFSKAWRLAGIRLGYLLAHDWVIEDLRKVRLPYHLDALTQAAGLAALDMADEVT
                     AHIAVLGAERDRVHAELAAMPDVTVWPSAANFLLLRTTMDGGALFEALLARGVLVRDF
                     SSRPRLEGCLRVSIGTPEENDRFLAALKESLA"
     gene            complement(3038..4354)
                     /gene="hisD"
                     /locus_tag="VNU01_10990"
     CDS             complement(3038..4354)
                     /gene="hisD"
                     /locus_tag="VNU01_10990"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWH33182.1"
                     /translation="MTTSTLRVVDLRGDRTDPRARLPRAAAERVAAARDAARRILDDV
                     RDEGDAAVRRWTRELDGWQAAGEAPLEVGLDEARRALEELPAELAEALRRAAEQVRWF
                     HEQAKPRDWMEKRNGAVLGVRHQPLRRVGVYVPGGRAAYPSTVLMTVVPARVAGVDEV
                     VLCTPPGRDGEVNRTILAAAALAGADRIFRVGGAQAIGAMAYGTASIPPCDKVVGPGN
                     VYVAEAKQIVASEGVCGIDAKAGATEVAIIADDSADPRVVAADLVAQAEHDPMAGCIL
                     ITPSEALAEAVEVALRTEVAGTKHAERVRQALDGQGAAVLVDDLDHAVEVANAFAAEH
                     LEVQTQDSVAVAERVRAAGAVFVGHATPVALGDYAAGPNHTLPTAGTARFTGGLRTDD
                     FYVPVNWVQYDEASLADLAPTVDALAAAEDLPAHARAVRIRLEGAP"
     gene            4449..5090
                     /locus_tag="VNU01_10995"
     CDS             4449..5090
                     /locus_tag="VNU01_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWH33183.1"
                     /translation="MRLPLFPLHVVLFPGRPLALHIFEPRYRAMLADVLAGDRRFGVV
                     AIRSGHEVGGPAEIHDVGTVAHLEDVELHADGRADVITRGEQRFRVVGTVPGKPYLLA
                     DVEALDDEAPGEHAGRLAQAVRAALKPYLCALGAPPELMELLPDDPVQLGYFAAASLQ
                     TDLPEQQRLLALDSAQGRLAATLRLLRREQGIARHFGTMGSLRPPSPAPTDLN"
     gene            complement(5156..6430)
                     /locus_tag="VNU01_11000"
     CDS             complement(5156..6430)
                     /locus_tag="VNU01_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWH33184.1"
                     /translation="MLSLAWALTLVLAAWVAKDYVRDGRSPAAAVGEPTTLSVELREF
                     AITPVELRAPAGAPLRIEVRNAGAIMHNLAVEGAGTTEDFPGGQRATLELPPLEEGAY
                     KLLCTIPGHEAAGMAATLTVGGDGAVAHDHADHALGAEEMARMHVEGVKAFPAPTEGR
                     GNQLLAPVSVAGGVAEYHLTADEISWETKPGVRKDGMAFNGMIPGPRIEGAVGDTIRV
                     VLHNKLDDAPTAAHFHGLLVPNDMDGVPGITQDPVMPGETFTYEFTLRNAGSHMYHSH
                     FDAAVQVPMGLLGAIVVRDGTEEPVDVDEVMVLNDGPLGFTINGKDFPATQPVVAALG
                     QKVRIRYMNEGLQIHPMHLHGMAQRVVARDGYPLPQPYLADTVLVGPGERIDVVVEAT
                     EPGVWAFHCHILNHAEAADGMFGMVTALIVEE"
     gene            6652..7500
                     /gene="lgt"
                     /locus_tag="VNU01_11005"
     CDS             6652..7500
                     /gene="lgt"
                     /locus_tag="VNU01_11005"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWH33185.1"
                     /translation="MSVLLPFAVIPPPPGDRIELGPFTVHYYGIAIAIGALIAVTLLR
                     KRFAARGGDPDLADRTAVWAVAAGLLGARLAYVSTHLDRFMERPWAILAIWEGGLAFF
                     GGLTFGALAAILYLRRHGASVADFADAAAPALPLAHAIGRWGNYFNEELYGTPTDVPW
                     ALDLSFQAQHVHPTFLYESLLNLTLAGTLLLADRGRALKRGTLVFLYLAGYGVIRFSM
                     ELLRTDTEWRLFGLSRNAYVALLVVLIGLVGARLRQRAPEPEPEPEEHAGADHDPSDG
                     PPAGLA"
     gene            7797..8780
                     /locus_tag="VNU01_11010"
     CDS             7797..8780
                     /locus_tag="VNU01_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33186.1"
                     /translation="MALTALTSVPAVAGSLGSLDGPLLSVTLGAPADWCTPDGALDAA
                     AMPAVVPPSRCDLDGRVVADHGVLLTVPAPGQKVGGEAMLLDGTTQVFELARRADGTL
                     VLGDVGDERADGADAGGHGDSGVNPCQDAAFQMSDYRESDERLWHYKASTTPPGLSIG
                     AVAQDLVNGFNAVTGSHNDCGLADEVDARQRYGGNTPHGTGINFEGRCPAQGDGHSVV
                     DFGNLPEETLARTCWRGIRTAQGWYEITEADVKIRSAAPVFTGDVQPGCAGARYSIEA
                     IVTHEAGHSFGLRHVPERDHPELTMSEQTPACSRAPVTLGLGDVEALRSVY"
     gene            8893..10071
                     /locus_tag="VNU01_11015"
     CDS             8893..10071
                     /locus_tag="VNU01_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH33187.1"
                     /translation="MGELRSAVDGVLGQDPCELADVELAERLVELEREIARLTAAQLA
                     ALAAFDARGAYQADGCGSAASWLRKMARVAPGDAAARVRAARRLRDEQPHVAAALADG
                     DISWPHARVLSEQIGRTAQRLRLRAGGQVDAIDAADEVVRSAEKLLVDAARTLDPRDT
                     AKVARRWQHLVDPDGFDRDAEHAFHRRYLNVSATWGGMVRIDGELDAEGGQTVLSAIQ
                     SLAAPDRPTDPRLPGQRRADALVELSRRVLDTGALPQSGGEKPHITVAISLDALEGRL
                     GHEPGELAKTGPISAETARRLACDAGITRIITGADSEPLDIGRRTRVIPSALRRALIH
                     RDQGCAWHGCDMPPEWTDAHHITHWADGGPTSLHNLVLLCRRHHRAVHESGQPLPQPP
                     "
     gene            complement(10086..10496)
                     /locus_tag="VNU01_11020"
     CDS             complement(10086..10496)
                     /locus_tag="VNU01_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HWH33188.1"
                     /translation="MSYALRMLETYPGDFGFDAAQLAAAIEALHDCAQACTACADACL
                     AEPQVADLVACIRLNLDCADVCATTARALSRQTEYKPELARALLEACVIACRACGDMC
                     AHHGEMGMEHCAVCAEACRRCAEACEALLAKASV"
     gene            10615..>10898
                     /locus_tag="VNU01_11025"
     CDS             10615..>10898
                     /locus_tag="VNU01_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWH33189.1"
                     /translation="MATSDLRTPGDVPPAPARPGRERGGLRLPPRRTKDGLVAGVASG
                     LAAGTGIDPFLLRWGFALLGLAGGVGVAAYLGAWALMDPPDAEAPRYAATP"
BASE COUNT         1352 a         4121 c         3954 g         1471 t
ORIGIN      
        1 ccggcgtcca gggcgagcgc ctcgggcagc gtgaagcggc ccgcgtacag cgccttgccg
       61 acgatcgcgc cgtcgacgcg ctcgtgcgcg gacgcgagcg cgcgcaaatc ctccaggctg
      121 gacacgccgc cgctcgcggt caccttggcg ctggtggcct cggccacctc gcgcaggcgc
      181 tcgacgttgg gcccggtgag catgccgtcc cggctgatgt cggtgaacac gaagcgcgcc
      241 acgcccatca tggtgaagcg gcgcagggcg tcgaacaggt cgccggactc ggtggtccag
      301 ccgcgcgcct tcaaggtggt gccctcggcg tccaggccca ccgcgacctt gtcgccgtac
      361 tcggcgacgg ccgccgccac gaagctcggc tcctccagcg ccatggtgcc gatcaccacg
      421 cggctggcgc cgtagtcgac cgagtcgcgc aggtccgcca tggagcggat gccgccggag
      481 gcctgcacgg ggatgccggt ggccttgacg atgtcggcga tgaggtgccg gttgcgcggc
      541 tcgccgcgga acgccgcgtc gaggtccacc acgtgcagcc actgggcgcc ctgctcggcg
      601 aagcgcacgg cggcctgcac cgggtcgtcg tcgtagaccg tctcggcgtc ctcgcggccc
      661 tgcaccaggc ggaccgcacg gccctccttg atgtcgaccg cgggatacag cgtgaagctc
      721 atgcgacctg ccctctgacc tcgtcgacga agttctccag catcctgcgg ccgacgtcgc
      781 cggacttctc ggggtggaac tgcgtgccca cgacactgcc cacgcgcacc acgcacggga
      841 agccgggccc gtagtcggcg gtggcgacga cgtgcgcgtc gtccgcgggg tcggcgtagt
      901 aggagtgcac gaagtaggcg tgcttcccgt ccacgccgtc gaccagcggg tcctcgcggg
      961 cggcctccac cgtgttccag cccatgtggg gcaccaccac gtcgccgggc aggcgccgca
     1021 cccggcccgg cagcaggccc agccccggcg tggcggggtc ctcctcgctg ccctggtaca
     1081 ggatctgcat gcccacgcag atgcccagca gcggcctgcc gcgcgcggac cagtcacgga
     1141 cgagctgctc gaagccggcc tcgcggaact gccgcgcgca ctggccgaag tggccgacgc
     1201 cgggcaccac gatggcgtcg gcgcccgccg cctcggcggc gtcggcggtg acgaacgcgt
     1261 ccgccccggc gcgcgccagg gccttctccg ccgagcgaag gttgccggcc tcgtagtcga
     1321 gcacggcgac gcggggaccc ggaccgctca tccccccagg gtgcccttcg tcgacgggat
     1381 cgcagagccg gtcacgcgca ccgcgcggcg cagcgccacc gcgaccgcct tgaacaccgc
     1441 ctcctggatg tggtggctgc cctcgccggc cagcttctgc acgtgcagcg tgcagcgcgc
     1501 gttggccacc agcgcctcga agaagtgctt ggtcagggtg gtctcgtacg tgccgaccag
     1561 ctccaccggg gtgtccgcgt cgtacaccag gtacgggcgc ccggacaggt ccacggcgac
     1621 gcgcaccagg gcctcgtcga tcggcacggt ggcgtcgccg aagcgctcca ccccggcctt
     1681 gtcgccgagc gcctccgcga gcgcctgccc gagcgcgatg cccgtgtcct cgacggtgtg
     1741 gtgcgcgtcg acgtgcaggt cgccgtccgt gcgcaccgtg aggttcaggc ggccgtggcg
     1801 gccgagctgg tcgagcatgt ggtcgaagaa cggcacgccc gtggagatct cacacgtgcc
     1861 gtcgccgtcg aggtcgacgc gcacctcgac cttggtctcc ttggtctggc ggaccaccga
     1921 tccggtccgt ggtgcgtcgc tcatgccagg ctctccttca gtgcggccag gaagcggtca
     1981 ttctcctccg gcgtgccgat cgagacgcgc aggcagccct ccagccgtgg ccgtgacgag
     2041 aagtcgcgta ccagcacgcc gcgggccagc agcgcctcga acagcgcacc gccgtccatc
     2101 gtcgtgcgca gcagcaggaa gttcgcggcg ctgggccaca ccgtgacgtc gggcatggcg
     2161 gccagctccg cgtgcacgcg gtcgcgctcg gcgcccagca ccgcgatgtg ggcggtgacc
     2221 tcgtccgcca tgtccagcgc cgccagcccg gcggcctgcg tcagcgcgtc caggtgatac
     2281 ggcaggcgca ccttgcgcag gtcctcgatc acccagtcgt gggcgagcag gtagcccagg
     2341 cggatgccgg ccagccgcca cgccttggag aaggtgcgcg tcaccacgac gcgcggcagc
     2401 tcgtccagga cggtcgtggc cgacacgccc ccggcgccgt cgccgtggtt ctgactgaac
     2461 tccacgtagg cctcgtcgac gatcaccagc gcgcggctcg cgtcgtgcag cgcgcgcacc
     2521 acgtccaggc cgaccggcat gccggtgggg ttgttcgggc tggtgacgca gatcagctcc
     2581 gggtcgtgcg cctcgacggc ggccagcgcg atctccgtgg tgagccggta gtcgccgtcc
     2641 aggtcggcct gcacgctgtc ggtcagcgcg atctgcgcgt acagcgggta cggcgagtag
     2701 ccgggccgga acagcagcag cgtgcgtccg ggcccgccgt aggcctggaa cagctgcaac
     2761 agcagctcgt tggagccgtt ggccgcccac acgcgctccg gcggcaggcc gaagcgctcg
     2821 ccgagggccg tgcgcagcgc ccacgcgcgc cggtccgggt agcggtgcag cggcagggtc
     2881 tccacgcgct cggccaggcg cttgaggtac tccgcgggcg gcgcgaacgg cgtctcgttg
     2941 gtgttgagcc ggaccggcac gtccagctgc ggcgcgccgt agggcaccac gtccgcgagg
     3001 tcgccgcgca cgggcacgcg cgacggcgtg tccccgctca cggcgcgccc tccaggcgga
     3061 tgcgcaccgc ccgggcgtgc gcgggcaggt cctcggcggc cgcgagcgcg tccacggtcg
     3121 gggcgaggtc ggctagcgac gcctcgtcgt actgcaccca gttcacgggc acgtagaagt
     3181 cgtccgtgcg cagcccgccg gtgaagcgcg ccgtgcccgc ggtgggcagc gtgtggttgg
     3241 gcccggcggc gtagtcgccc agcgcgaccg gggtggcgtg gcccacgaag accgccccgg
     3301 ccgcgcgcac gcgctccgcc acggcgacgg agtcctgcgt ctgcacctcc aggtgctccg
     3361 cggcgaacgc gttggcgacc tccacggcgt ggtcgaggtc gtcgaccagc acggcggcgc
     3421 cctgcccgtc cagcgcctgg cgcacgcgct cggcgtgctt ggtgccggcg acctcggtgc
     3481 gcagcgccac ctcgacggct tcggccagcg cctccgacgg cgtgatcagg atgcagcccg
     3541 ccatcgggtc gtgctcggcc tgggcgacca ggtcggcggc gacgacgcgc gggtcggcgc
     3601 tgtcgtcggc gatgatcgcg acctcggtgg cgcccgcctt cgcgtcgatc ccgcacacgc
     3661 cttccgacgc cacgatctgc ttggcctcgg ccacgtacac gttgccgggg ccgaccacct
     3721 tgtcgcaggg cgggatcgac gcggtgccgt aggccatggc gccgatcgcc tgggcgccgc
     3781 ccacgcggaa gatgcggtcc gcgcccgcca gcgcggcggc cgcgaggatg gtgcggttga
     3841 cctcgccgtc gcgtcccggc ggcgtgcaca gcacgacctc gtcgacgccg gcgacgcgcg
     3901 ccggcaccac cgtcatgagc accgtggacg ggtaggcggc gcggccgccc ggcacgtaca
     3961 cgccgacgcg ccgcagcggc tggtggcgca cgccgagcac ggcgccgttg cgcttctcca
     4021 tccagtcgcg gggcttggcc tgctcgtgga accagcgcac ctgctcggcg gcgcggcgca
     4081 gcgcctccgc gagctcggcg ggcagctcct ccagggcgcg gcgcgcctcg tcgagcccga
     4141 cctccagggg cgcctccccg gcggcctgcc agccgtccag ctcccgggtc cagcggcgca
     4201 ccgccgcgtc gccctcgtcg cgcacgtcgt cgaggatgcg ccgggcggcg tcgcgcgcgg
     4261 cggcgacgcg ctccgcggcg gcgcgcggca agcgcgcgcg cgggtccgtg cggtcgccgc
     4321 gaaggtcgac gacccggagg gtgctggtgg tcatgcaggg actgtagcga ctgcggcgcc
     4381 gggcgagcca agccgccgcc cgcgccgatc cggtgcgatc gagcgccgcc gacgtacgct
     4441 tggccgggat gcggctgccg ttgttccccc tccacgtcgt cctgttcccg ggcaggccgc
     4501 tggcgctgca catcttcgag ccccgctacc gcgcgatgct ggcggacgtg ctggcgggcg
     4561 accgccgctt cggcgtggtg gcgatccgca gcggccacga ggtgggcggg ccggcggaga
     4621 tccacgacgt cggcaccgtg gcccacctgg aggacgtcga actgcacgcc gacggccgcg
     4681 ccgacgtgat cacccgcggc gagcagcgct tccgcgtcgt cgggacggtg cccggcaagc
     4741 cgtacctgct cgccgacgtg gaggcgctgg acgacgaggc gccgggcgag cacgccggca
     4801 ggctggccca ggcggtgcgc gcggcgctga agccctacct gtgcgcactg ggcgcgccgc
     4861 cggagctcat ggagctcctg cccgacgacc ccgtgcagct cggctacttc gcggccgcca
     4921 gcctgcagac cgacctgccc gagcagcagc gcctgctggc gctggactcg gcgcagggcc
     4981 ggctggccgc gacgctgcgc ctgctgcgcc gcgagcaggg catcgccagg cacttcggga
     5041 cgatgggctc gctgcggccg ccgtcgcccg cgccgaccga cctcaactga tctgtctcgg
     5101 agcggttcgc gtgcctgcgc ggcacttcgt gccgctgacc gcgaaccggc gcgcctcact
     5161 cctccacgat cagggcggtg accatgccga acatgccgtc ggcggcctcg gcgtggttga
     5221 ggatgtggca gtggaacgcc cacacgcccg gctcggtggc ctccaccacc acgtcgatgc
     5281 gctcaccggg cccgaccagg accgtgtcgg ccaggtacgg ctggggcagc gggtagccgt
     5341 cgcgggcgac cacgcgctgt gccatgccgt gcaggtgcat ggggtggatc tgcagcccct
     5401 cgttcatgta gcggatgcgg accttctggc ccagcgcggc gacgaccggc tgcgtggccg
     5461 ggaagtcctt gccgttgatg gtgaagccga gcggcccgtc gttgagcacc atcacctcgt
     5521 cgacgtcgac cggctcctcc gtgccgtcac ggacgacgat cgcgccgagc agccccatgg
     5581 gcacctggac ggccgcgtcg aagtgcgagt ggtacatgtg cgacccggcg ttgcgcagcg
     5641 tgaactcgta ggtgaaggtc tccccgggca tcaccgggtc ctgggtgatg cccggcacgc
     5701 cgtccatgtc gttgggcacc agcaggccgt ggaagtgggc cgccgtgggc gcgtcgtcca
     5761 gcttgttgtg caggacgacg cggatcgtgt cgccgacggc cccctcgatg cgcgggccgg
     5821 ggatcatccc gttgaacgcc atgccgtcct tgcgcacgcc gggcttggtc tcccacgaga
     5881 tctcgtcggc ggtgaggtgg tactccgcca cgccgccggc cacggacacg ggcgcgagca
     5941 gctggttgcc ccgcccctcc gtcggcgccg ggaaggcctt gacgccctcg acgtgcatcc
     6001 gcgccatctc ctccgcgccc agggcatggt cggcgtggtc gtgcgcgacc gcgccgtccc
     6061 cgcccacggt cagcgtcgcc gccatgccgg cggcctcgtg cccggggatg gtgcacagca
     6121 gcttgtacgc gccctcctcc aggggcggca gctccagcgt cgcgcgctgc ccgccgggga
     6181 agtcctcggt cgtgccggcg ccctccacgg ccaggttgtg catgatcgcc ccggcgttgc
     6241 gcacctcgat gcgcagcggc gcgcccgcgg gcgcccgcag ctcgaccggc gtgatcgcga
     6301 actcgcgcag ctccacggac agcgtggtgg gctcgccgac cgccgcggcc ggcgaccggc
     6361 cgtcgcggac gtagtccttg gcgacccacg cggccaggac gagtgtcagg gcccaggcca
     6421 gcgacagcac ggcacgggac ggttgcgcac ggaacgacat cgggcacctc gcggctcggg
     6481 cggcacccac ggccgccgac tgtccaccac gctggcgagc cgaggccccg tccggcagtg
     6541 cccacggtcc cgcggcccgg ccgcttccgg cccagcgcgg gcgggacctc cggccctgcg
     6601 tccccccgac cgccgggccg cccggccgcg cagcctctag gatccgcccc catgagcgtt
     6661 ctgctgccgt tcgcggtcat cccgccgccc cctggcgacc ggatcgaact gggccccttc
     6721 accgtccact actacggcat cgcgatcgcc atcggcgcgc tgatcgcggt gacgctgctg
     6781 cgcaagcgct tcgcggcgcg cggcggggac cccgacctgg ccgaccgcac cgcggtgtgg
     6841 gcggtcgcgg ccggcctgct gggcgcgcgg ctcgcctacg tctccacgca cctggaccgc
     6901 ttcatggagc gcccgtgggc gatcctggcg atctgggagg gcggcctggc gttcttcggc
     6961 ggcctgacgt tcggggcact ggccgcgatc ctgtacctgc gccgccacgg cgcgtccgtg
     7021 gccgacttcg ccgacgcggc cgcgccggcc ctgccgctgg cgcacgcgat cggccgctgg
     7081 ggcaactact tcaacgagga gctctacggc acgcccaccg acgtgccgtg ggccctggac
     7141 ctgtccttcc aggcccagca cgtccacccg acgttcctgt acgagtcgct gctgaacctc
     7201 acgctggcgg ggacgctcct gctggccgac cgcgggcggg cgctcaagcg cggcacgctg
     7261 gtgttcctgt acctggccgg ctacggggtc atccgcttca gcatggagct gctgcgcacg
     7321 gacaccgagt ggcggctgtt cggcctgtcg cgcaacgcct acgtcgcgct cctggtcgtg
     7381 ctgatcggcc tggtcggcgc gcggctgcgc cagcgcgcgc ccgagccgga gccggagccg
     7441 gaggagcacg cgggtgccga ccacgatccg tcggacgggc cacctgccgg cctcgcctga
     7501 gcgggcggtg ccatgcccgg cgggagccgg ctgggtgctg cggttctggg cacagggcct
     7561 cgacaagctc gccgccatct gaaacagtca attccgtaaa aaccgggcga ggtgttcgcc
     7621 tcctccggcg gcgttgccta cgctgcccgg gcagtctcag cccacggcgc cttgcgccgc
     7681 ggccaccggc cgtgcgtccg gcgcctcacg actgaaggtc tctcgtggtg cgcttctccg
     7741 gggcacgcgc gttgcgcgcg ggccgtagct ccctccgtgc cgccgccctc tgggtggtgg
     7801 ccctgacagc gctgacctcg gtcccggccg tggccgggtc gctggggtcg ctcgacgggc
     7861 cgctgctgtc cgtgaccctc ggtgcccccg cggactggtg cacccctgac ggcgcgctcg
     7921 acgccgccgc catgcccgcc gtggtgcccc cctcgcgctg cgacctggac ggccgggtgg
     7981 tcgccgacca cggggtgctc ctcacagttc ccgcgcccgg tcagaaggtc ggcggcgagg
     8041 cgatgctgct ggacggcacc acgcaggtgt tcgagctggc gcgccgcgcc gacggcacgc
     8101 tggtactcgg cgacgtgggc gacgagcgcg ccgacggggc cgacgccggg gggcatggcg
     8161 acagtggcgt gaacccgtgt caggacgcgg cgttccagat gtcggactac cgcgagtccg
     8221 acgagcgcct gtggcactac aaggcctcga ccacgccgcc ggggctgtcc atcggcgcgg
     8281 tggcgcagga tctcgtcaac ggcttcaacg ccgtcaccgg ctcgcacaac gactgcggcc
     8341 tggccgacga ggtcgacgcg cgccagcgct acggcggcaa caccccgcac ggcacgggca
     8401 tcaacttcga gggccgctgc cccgcgcagg gcgacggcca ctccgtggtg gacttcggca
     8461 acctcccgga ggagacgctg gcccggacgt gctggcgcgg catccgcacg gcgcagggct
     8521 ggtacgagat caccgaggcg gacgtgaaga tccgctccgc ggcgcccgtg ttcaccggag
     8581 acgtccagcc cggctgcgcg ggcgcccgct acagcatcga ggcgatcgtc acgcacgagg
     8641 ccgggcacag cttcggcctg cggcacgtgc cggagcgcga ccatcccgag ctgacgatgt
     8701 cggagcagac gccggcgtgc tcgcgcgccc cggtcacgct gggcttgggc gacgtcgagg
     8761 cgctgcgcag cgtctactag gcagcgccgc gtccgcgccc ctgagaagtt tctcggaaaa
     8821 gttttctgtg gcctgtgcgc aagtgggttt cggggccgtt tttcgtcaca ccctcgtggg
     8881 agtgttggcg gcatgggtga gctgcggtct gccgtcgacg gggtcctggg ccaggaccct
     8941 tgtgagcttg ccgacgtgga gctggctgag cggctggtgg agctggagcg cgagatcgcc
     9001 cggctgaccg cggcgcagct ggccgcgctg gcggccttcg acgcgcgcgg cgcctaccag
     9061 gccgacggct gcggcagtgc cgcgtcatgg cttcgcaaga tggcccgggt ggcgcccggc
     9121 gacgcggccg cgcgggtgcg tgccgcacga aggctgcgcg acgagcagcc ccacgtcgcc
     9181 gccgcgctgg ccgacgggga catctcctgg ccccacgcca gggtcctgtc ggagcagatc
     9241 ggccgcacgg cgcaacggct gcggctgcgc gccggcggcc aggtcgacgc gatcgacgcg
     9301 gcagacgagg tcgtgcggtc ggcagagaag ctgctcgtgg acgccgccag gacactcgac
     9361 ccgcgcgaca ccgccaaagt cgcccgccgc tggcagcacc tggtcgaccc cgacgggttc
     9421 gaccgcgacg ccgagcacgc cttccaccgc cgctacctca acgtgtcggc cacctggggc
     9481 ggcatggtcc gcatcgacgg cgagctcgac gccgaaggcg gccagaccgt gctgagcgcc
     9541 atccaatccc tcgccgcgcc cgacaggccg accgacccgc gcctgcccgg ccaacgccgc
     9601 gccgacgcac tggtcgaact gtcccgccgg gtcctggaca ccggcgccct gccccagtca
     9661 ggcggcgaga agccccacat caccgtggca atcagcctcg acgcactcga aggacgcctc
     9721 ggccacgagc cgggggaact ggccaagacc gggccgattt ccgccgagac cgcccgccgt
     9781 ctcgcctgcg atgcggggat cacccgcatc atcaccgggg cggactccga gcccctcgac
     9841 atcggccggc gcacccgcgt catcccatcc gcgctccgcc gcgccctgat ccaccgcgac
     9901 caaggctgcg cctggcacgg ctgcgacatg ccccccgaat ggaccgacgc ccaccacatc
     9961 acacactggg ccgacggcgg ccccacttcg ctgcacaact tggtcctgct ctgccgccgc
    10021 caccaccgcg ccgtccacga aagcggccaa cccctccccc agccgccgta agtcgcgagc
    10081 ggcggttaga cggaggcctt ggccaggagc gcttcgcagg cctcggcgca gcgccggcac
    10141 gcctcggcgc acaccgcgca gtgctccatg cccatctcgc cgtggtgcgc gcacatgtcg
    10201 ccgcacgccc ggcaggcgat gacgcacgcc tcgagcagcg cgcgtgcgag ctcgggcttg
    10261 tactccgtct gccgcgacag cgcccgggcc gtggtcgcgc agacgtcggc gcagtccagg
    10321 ttcaggcgga tgcaggcgac cagatcggcc acctgcggtt cggcgagaca cgcgtcggcg
    10381 caggccgtgc acgcctgggc gcagtcgtgc agcgcctcga tcgcggcggc gagctgcgcg
    10441 gcgtcgaagc cgaagtcgcc gggataggtc tcgagcatgc gcagggcgta ggacacggtc
    10501 ttcctccatg cggtcggtgg tcgtccaggc gacgcctacc ccgcaccgcg gcttgcgcat
    10561 gcctcggggt catccccgag tgccctgcgg gtggcgctgt gccagcatgg cggcgtggcg
    10621 acctcagatc tccgcacgcc gggcgatgtc ccgcctgcgc ctgcccgccc cgggcgcgag
    10681 cgcggcgggc tccgcctgcc gccgcggcgg acgaaggacg gcctggtggc cggtgtcgcc
    10741 tccggcctgg ccgccggcac cgggatcgac cccttcctgt tgcgctgggg ttttgcgctg
    10801 ctggggctcg cagggggtgt cggcgtcgcc gcgtacctgg gcgcctgggc gttgatggac
    10861 cctcccgacg cggaggcgcc caggtacgcg gcgacgcc
//
LOCUS       DATMKC010000375         6334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_130168, whole genome shotgun sequence.
ACCESSION   DATMKC010000375 DATMKC010000000
VERSION     DATMKC010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130168"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VNU01_11030"
     CDS             <1..1448
                     /locus_tag="VNU01_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH33190.1"
                     /translation="LAAAVLGTAFGRVVAHRGAGVRAAHVAVSVVAIVTAVVVLVPRS
                     TPALSADIRTSPAGHDAIDVEVLLTPADGADGAERFQVMAWQGGELAVAHLREVERGR
                     FVTDEPVPVGGDGKALVRIAHGGALGAIPISMPADPAIGADEVPLLPRRQGPFLAESH
                     VLLREAHTGPAWPAVVGYTFVALGVGTVIGLLLAATVGLDRRRRALGWRNGDGTLAGT
                     RVVLTGCEGGIGLAAKRALEGQGARVVGIDVRAGGRDCLVADLRDPDAVRAAIDEAAA
                     RLGGIDVLVANAGIGAADETARPPSAEARDVMDVNCFGSWEVIGQAMPHLRRGGGHVV
                     VVGSGLARVNMPYSAAYTASKRALVGYADVLRLEEDGAVTVSVVQPAYIRTPIHDGPA
                     AHGASLDGVARVETVQDAAAAIVTACETHRRELGSSPVTTLQLWAARHWPAVVDRAVA
                     QRRRQVERRRPRPVFPDRSGDRSRRAGKVQA"
     gene            1445..2464
                     /locus_tag="VNU01_11035"
     CDS             1445..2464
                     /locus_tag="VNU01_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH33191.1"
                     /translation="MKLAKVLVRVSLGALALAAAGVAAMMRRWAATPDLSGGPLVLPQ
                     GSERVVERPDGVRLAVLEAGEGGSPTFVMPHCWTGDRRVWGPVARRLVKAGAHVVVYD
                     HRGHGRSTLECGDYSLEALGDDLAAVLDSVTTERVLLVGHSMGGFTIQSFLRRHPEAA
                     ARRVAGVGFVASATDGQRPTSRLRAAITLWLMSSPLSDFGLRGPLATFWTRFTVGRTV
                     CAAHLDAVIQPYLATPPAARRAFLEAIYGMDFSDVLPGVRVPALLVSGGRDQLTVPGR
                     MRRMAELMPHARFVSLPDAGHMLPQEEPDRLTALLLDLAAEAGAGAPVEPRVLDLSDL
                     SEQIS"
     gene            2461..3945
                     /locus_tag="VNU01_11040"
     CDS             2461..3945
                     /locus_tag="VNU01_11040"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWH33192.1"
                     /translation="MIEHFRIAIVGSGFAGLGLAIQLKRSGRDDFVVLERGDDVGGTW
                     RDNTYPGAACDVPSLLYSFSFAPNPGWSRSFSPQEEIQDYLRDCADRFGVRPHIRFGN
                     RVDEARWDDEAQLWRIRTSAGELTAQFLMGGMGGLSEPSVPDLPGLERFGGTTFHSAR
                     WDHGYGLRGKRVAVVGTGASSIQFVPEIQPLVERLDVYQRTPAWIVPRWDRDLTRVER
                     ALYRRFPALQRLVRALIYWGREFYAIPFMHHRKRFTSPPERVARRHLRKQVPDPDLRA
                     KLTPDYAAGCKRILISNDWYPALGKPNVDLVTAGIAEVTETGVVDRDGVERPVDAIIF
                     GTGFHVTDFPHGRYIFGRDGRALTEHWREGAQAYLGMAAPGFPNFFFLTGPNTGLGHT
                     SMVFMMECQFAYLLDMLGVVEERGIATVEVRPDVVAAFNTRLHERMPQTVWSSGGCAS
                     WYLDEHGRNTTLWPDFTWKFRLRTRRFSPADYDLGRRRVRVPAA"
     gene            4029..4811
                     /locus_tag="VNU01_11045"
     CDS             4029..4811
                     /locus_tag="VNU01_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWH33193.1"
                     /translation="MPQATESCVHLPPPGHDVIGPDRFRQPGLVAVESIGPGMPVQRV
                     GPVFMVHDSTFEPGCGIGHHPHRGMERLFYILEGAVAHDDAQNGITGHMGTGDLGILT
                     EGRQGMLHSERNAAQGRTRCWILVYPCEPLVEHAAFDAIRADAMRVTEGQVAGGSRRT
                     THVVEPGDSRLHGDIRLFGDTTLDEGGEFEFALNADETGLLFVVDGDVAMVRAHDEVV
                     LSPTHTVAWGTADHGREVMLRALRPARLLHVVMGPGDGLVRR"
     gene            4982..>6334
                     /locus_tag="VNU01_11050"
     CDS             4982..>6334
                     /locus_tag="VNU01_11050"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HWH33194.1"
                     /translation="MSATSEIGTAAQATVATAPNGRQRRLETAEQRWARMPFITTGED
                     YVASLYGRGTSLYLFGEKIDEPADHPVIRPSIEALKATYDLATERPELATAISPYTGE
                     PVNRFLHIVESPDDLVAKARMQRELGRRTGTCFQRCVGLDAISTLHSITFELDAAHGT
                     DYQRRFIEFLTQAQRRNVLIAGGMTDPKGDRSKRPHQQADADLFLHVSGRNSHGVFVR
                     GAKAHMTGGWNQHWIMLMPTMNLSAEDRDYAIVCAVPGDAEGITYVYGRQASDTRAME
                     PGTMDQGNARFGGQEVLVHFDDVFVPYDHVLLDGEYEFAQELVARFTTYHRASYVCKT
                     GLGDVLTGAAAQAAEWNGVPGASHIADKLVEMTHLNETIYSSAIASAHEASELPSGVF
                     MNEPMLANVAKHNVTRFPYEIARLAQDIAGGMTDPKGDRSKRPHQQADADLFLHVSGR
                     N"
BASE COUNT          838 a         2318 c         2315 g          863 t
ORIGIN      
        1 cgctggcggc cgccgtgctc ggcaccgcgt tcggccgcgt ggttgcccac cgcggggcgg
       61 gagtgcgcgc ggcgcatgtc gccgtgtcgg tggtcgcgat cgtgaccgcc gtcgtggtgc
      121 tggtgcccag gtcgacgccg gctctgtccg ccgacatccg cacctcgccg gcggggcacg
      181 acgcgatcga cgtcgaggtg ctcctgacgc cggcggacgg cgccgacggc gccgagcggt
      241 tccaggtcat ggcatggcag ggcggcgagc tcgccgtcgc gcacctgcgc gaggtcgagc
      301 gcggccggtt cgtcacggac gagcctgtgc ccgtcggcgg cgatggcaag gcgctcgtgc
      361 gcatcgccca cggcggcgcg ctgggcgcca tccccatctc catgccggcc gacccggcca
      421 tcggcgcgga cgaggtgccg ctgctgccgc gccggcaggg acccttcctg gcggagagcc
      481 acgtgctgct gcgcgaggcc cacacaggac cggcgtggcc ggccgtggtc ggctacacct
      541 tcgtggccct gggcgtgggc accgtgatcg gcctgctgct cgccgcgacg gtcggcctgg
      601 accgccgccg gcgcgcgctc gggtggcgca acggagacgg cacgctggcc ggcacgcgcg
      661 tggtgctcac cgggtgcgag ggcggcatcg gcctggctgc caagcgcgcc ctggagggcc
      721 agggcgcgcg ggtcgtcggc atcgacgtgc gcgccggggg ccgtgactgc ctggtggcgg
      781 acctccgcga tcccgacgcc gtgcgtgcgg ccatcgacga ggccgccgcg cgcctgggcg
      841 gcatcgacgt gctggtggcc aacgccggga tcggcgcggc ggacgagacc gcgcgccccc
      901 cgtcggcgga ggcgcgcgac gtcatggacg tcaactgctt cggctcctgg gaggtgattg
      961 gccaggccat gccccacctg cgccgcggcg gcgggcacgt ggtggtggtc ggctccggcc
     1021 tggcccgcgt gaacatgccc tacagcgccg cctacaccgc cagcaagcgc gcgctggtgg
     1081 gctacgccga cgtgctgcgg ctggaggagg acggcgccgt caccgtgagc gtcgtgcagc
     1141 ccgcctacat ccgcacgccc atccacgacg gcccggcggc gcacggcgcc agcctggacg
     1201 gcgtggcgcg cgtggagacc gtccaggacg ccgcggccgc tatcgtcacc gcctgcgaga
     1261 cgcacagacg ggagctgggc agctcgccgg tcacgacgct gcagctgtgg gcggcgcgcc
     1321 actggccggc cgtggtggac cgcgccgtgg cgcagcggcg ccgccaggtc gagcggcggc
     1381 gcccgcggcc ggtgttcccg gaccggtccg gcgaccggtc gcgacgagcg gggaaggtgc
     1441 aggcatgaag ctggccaagg tcttggtacg cgtgtcgctg ggggcgctgg cgctggcggc
     1501 ggcgggcgtg gccgcgatga tgcgccgctg ggccgccacc cccgatctgt ccggcgggcc
     1561 gctggtgctc ccccagggca gcgaacgcgt cgtcgagcgc ccggacggcg tccggctggc
     1621 cgtgctggag gcgggggagg gcggctcgcc gacgttcgtc atgccccatt gctggaccgg
     1681 ggaccgccgc gtgtggggcc cggtggcgcg gcgcctggtg aaggccggcg cccacgtcgt
     1741 ggtgtacgac caccgcgggc acgggcgctc gacgctggag tgcggcgact actcgctgga
     1801 ggcgctcggc gacgacctcg ccgccgtgct ggacagcgtg accaccgagc gcgtgctgct
     1861 cgtcggccat tcgatgggtg gcttcaccat ccagtcgttc ctgcggcgtc acccggaagc
     1921 cgcggcgcgc cgggtcgccg gcgtcggctt cgtggcctcc gccacggacg ggcagcgccc
     1981 cacgtcacgg ctgcgggccg cgatcaccct gtggctcatg agttcgccgc tgtccgactt
     2041 cggcctcagg ggcccgctcg cgaccttctg gacgcgcttc acggtgggcc gcacggtgtg
     2101 cgcggcgcac ctggacgcgg tgatccagcc gtacctcgcc acgccgcccg ccgcgcgccg
     2161 cgcgttcctg gaggcgatct acggcatgga cttcagcgac gtgctgccgg gcgtgcgcgt
     2221 gccggcgctg ctggtctccg gcgggcgcga ccagctcacc gtgcccgggc gcatgcgccg
     2281 catggcggag ctcatgccgc acgcgcgctt cgtgtcgctg cccgacgccg gccacatgct
     2341 cccgcaggag gagcccgacc gtctcacggc gctgctgctg gacctggcgg ccgaggccgg
     2401 cgccggtgcc cccgtggagc cccgtgtcct cgacttgagc gacctgtcgg agcagatctc
     2461 gtgatcgagc acttccgcat cgccatcgtc ggcagcgggt tcgccggcct cggcctggcg
     2521 atccagctca agcgctccgg ccgcgacgac ttcgtggtgc tggagcgcgg cgacgatgtc
     2581 ggggggacct ggcgcgacaa cacctatccc ggcgcggcct gcgacgtgcc atcgctgctg
     2641 tactcgttct ccttcgcgcc gaaccccggc tggagccgct cgttctcgcc gcaggaggag
     2701 atccaggact acctgcgcga ctgcgccgac cgcttcggcg tgcgcccgca catccgcttc
     2761 ggcaaccgcg tggacgaggc gcgctgggac gacgaggcgc agctctggcg catccgcacg
     2821 agcgccggcg agctcaccgc gcagttcctc atgggcggca tgggcgggct gagcgagccg
     2881 tccgtgccgg acctgccggg cctcgagcgc ttcgggggca ccacgttcca ctcggcgcgc
     2941 tgggaccacg gctacggcct gcgcggcaag cgcgtcgccg tcgtcggcac cggcgcctcg
     3001 tcgatccagt tcgtgccgga gatccagccc ctggtggagc gcctggacgt ctaccagcgc
     3061 acgccggcgt ggatcgtgcc gcgctgggac cgcgacctga cacgcgtgga gcgcgcgctg
     3121 taccggcgct tcccggcgct gcagcggctg gtgcgcgccc tcatctactg ggggcgggag
     3181 ttctacgcga tcccgttcat gcaccaccgc aagcgcttca ccagcccgcc ggagcgcgtc
     3241 gcgcggcgtc acctgcgcaa gcaggtcccg gatccggacc tgcgcgccaa gctgaccccg
     3301 gactacgcgg ccggctgcaa gcgcatcctg atctccaacg actggtatcc ggcgctgggc
     3361 aagcccaacg tggatctggt caccgccggc atcgccgagg tcaccgagac gggcgtggtc
     3421 gaccgcgacg gcgtggagcg gccggtggac gccatcatct tcggcaccgg cttccacgtg
     3481 accgacttcc cgcacgggcg ctacatcttc ggacgggacg ggcgcgcgct caccgagcac
     3541 tggcgcgagg gcgcccaggc ctacctgggc atggccgcgc ccggcttccc aaacttcttc
     3601 ttcctgaccg ggcccaacac cggcctgggc cacacctcga tggtgttcat gatggagtgc
     3661 cagttcgcct acctgctcga catgctcggt gtggtggagg agcgcggcat cgccaccgtg
     3721 gaggtgcgtc ccgacgtggt cgcggcgttc aacacgcggt tgcacgagcg catgccgcag
     3781 acggtgtgga gcagcggcgg ctgcgcgagc tggtacctgg acgagcacgg ccgcaacacc
     3841 acgctgtggc cggacttcac ctggaagttc cgcctgcgca cgcgccgctt ctccccggcc
     3901 gactacgacc tggggcggcg gcgggtgcgg gtgccggccg cgtagggccg gtgccgcgcg
     3961 tgccaggatt ggacgcggtg cccggcgggg caggaaccct cgcacgatcc cgtccaagga
     4021 gttcccgcat gccgcaggcg accgagtcgt gcgtccatct cccgcccccc gggcacgacg
     4081 tcatcgggcc ggaccgcttc cgccagcccg gcctggtcgc ggtcgagtcg atcgggcccg
     4141 gcatgccggt gcagcgcgtc gggcccgtgt tcatggtgca cgactcgacg ttcgagcccg
     4201 gctgcggcat cggccaccat ccccaccggg gcatggagcg gctgttctac atcctggaag
     4261 gcgccgtggc gcacgacgac gcgcagaacg gcatcaccgg gcacatgggc accggcgacc
     4321 tcgggatcct cacggaaggc cgccagggga tgctgcactc cgagcgcaac gccgcccagg
     4381 ggcgcacgcg ctgctggatc ctggtgtatc cgtgcgagcc gctcgtggag cacgccgctt
     4441 tcgacgcgat ccgcgcggac gccatgcgcg tcaccgaggg ccaggtggcg ggagggagcc
     4501 gccgcacgac gcacgtggtg gagccgggcg acagccgcct gcacggggac atccgacttt
     4561 tcggcgacac gaccctggac gagggcggcg agttcgagtt cgcgctcaac gccgacgaga
     4621 ccggcctgct gttcgtggtg gacggcgacg tggcgatggt gcgcgcgcac gacgaggtgg
     4681 tcctgtcgcc cacgcacacg gtggcgtggg gcacggccga ccacggccgt gaggtcatgc
     4741 tgcgggcact gcgcccggcg cgcctgctgc acgtcgtgat gggccccggc gacggcctgg
     4801 tccgccgcta gcccccgcgc cccgtactcc aacctggagg atcccgtgcg gattgggagg
     4861 atcgcttccg tattcggaag cgatcctcca aggtggccac gatcttccag gttggggtct
     4921 gttgggcggg cggcggcacg cgatactggg ggtccttcca tcgagtgagg cggcgagagc
     4981 catgagcgcg accagcgaga tcgggacggc ggcgcaggcg acggttgcca cggcgcccaa
     5041 cggcaggcag cggcggctgg agacggccga gcagcgctgg gcgcgcatgc cgttcatcac
     5101 cacgggcgag gactacgtcg cctcgctgta cggacgcggc accagcctgt acctgttcgg
     5161 cgagaagatc gacgagcccg ccgaccatcc cgtcatccgc ccgtccatcg aggcgctgaa
     5221 ggccacctac gacctggcca ccgagcgccc cgagctcgcc accgcgatca gcccctacac
     5281 gggcgagccg gtcaaccgct tcctgcacat cgtggagtcc cccgacgacc tggtcgccaa
     5341 ggcgcgcatg cagcgcgagc tgggccggcg caccggcacg tgcttccagc gctgcgtggg
     5401 cctggacgcc atctccacgc tgcactcgat caccttcgag ctcgacgcgg cccacgggac
     5461 cgactaccag cggcgcttca tcgagttcct cacccaggcg cagcggcgca acgtgctcat
     5521 cgccggcggc atgaccgacc ccaaggggga ccgctccaag cgcccccacc agcaggcgga
     5581 cgccgacctg ttcctgcacg tgagcgggcg caactcccac ggcgtgttcg tgcgcggcgc
     5641 gaaggcgcac atgaccggtg gctggaacca gcactggatc atgctgatgc cgaccatgaa
     5701 cctgtcggcc gaggaccgcg actacgccat cgtctgcgcg gtccccggcg acgccgaggg
     5761 catcacctac gtctacggcc gccaggcgtc cgacacccgc gcgatggagc cggggaccat
     5821 ggaccagggc aacgcccgct tcggcgggca ggaggtgctg gtccacttcg acgacgtgtt
     5881 cgtgccctac gaccacgtcc tgctggacgg cgagtacgag ttcgcgcagg aactggtcgc
     5941 ccggttcacc acgtaccacc gcgccagcta cgtctgcaag accggtctcg gcgacgtgct
     6001 caccggcgcg gccgcgcagg ccgcggagtg gaacggcgtg ccgggcgcct cgcacatcgc
     6061 cgacaagctc gtggagatga cccacctcaa cgagacgatc tactcctcgg ccatcgcctc
     6121 ggcgcacgag gccagcgaac tgccgtcggg cgtgttcatg aacgagccga tgctggccaa
     6181 cgtcgccaag cacaacgtga cgcgcttccc gtacgagatc gcgcgcctgg cgcaggacat
     6241 cgccggcggc atgaccgacc ccaaggggga ccgctccaag cgcccccacc agcaggcgga
     6301 cgccgacctg ttcctgcacg tgagcgggcg caac
//
LOCUS       DATMKC010000376         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_319234, whole genome shotgun sequence.
ACCESSION   DATMKC010000376 DATMKC010000000
VERSION     DATMKC010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319234"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1432
                     /locus_tag="VNU01_11055"
     CDS             290..1432
                     /locus_tag="VNU01_11055"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH33195.1"
                     /translation="MTFELARLCLPYGEAALPEDVSERDIRLLHFDGRRPRDITVRVN
                     VRGDSVCGETDGFSPFALAVPEISAGPTLERLGSADAIRSASQVAGSRFRSGVPVVYV
                     ASGETYADVLALGPVATPSGGPILLTHRDQLPEATREMLLRLMPGRIVILGGHGAISA
                     VVEQALRQFAADGVSRLSGFDRYETASALLAASFPVATPVVFIATGENFPDAIAGGAA
                     AARLNAPVVLVQPDALPAASAAELARLRPERIYVLGGSGAVSAGVLVRLAEFASSSVE
                     RLAGADRFHTAVRISHEFFPRGASTVYLAAGEEFGAALAAVPAAARDSAPILLTRSSQ
                     IPAVVRDELERLRPERIVILGARGATAAMLREDVAELQSRWRVQRR"
     gene            complement(1429..>2813)
                     /locus_tag="VNU01_11060"
     CDS             complement(1429..>2813)
                     /locus_tag="VNU01_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33196.1"
                     /translation="PPDSPLPGALDNVHRGVRGLELRRGGEVARYSPTWMLATNRSFA
                     RVLELFAEARDLEDLAGVEASVFDAEQFLVLLGDGGVRARRLFRGNETVRAEDVDAAS
                     VAATARRLGDWLARAVHPDGRMTYQWFPSRGEESQGDNTVRQWMATAALGRYAAWRDD
                     ERVWALTRRNVARNLDAYYTEQLGLGLIEEGPDKVKLGAVALAGRALAEHPDRPRLAP
                     VIDALEATTVHLAEPDGSFRTWFRPADRDDQHNFYPGEALYFWAHRYAERRDPATLER
                     LMRAFRFYRGWHLDPANRNPAFVPWHTQAYVETWRHTRSDELATFVFEMNDWLLDMQQ
                     WDSAPYPDLRGRFYDPDRPGYGPPHASATGVYLEGLADAFWLAREVGDDGRAERYRVA
                     LVRGIRSVMQLEFADDADLYYVSDRARVRGGVRTEAYDNRIRVDNVQHNLMALLKVLE
                     RFGPADYRPS"
BASE COUNT          343 a          996 c         1055 g          419 t
ORIGIN      
        1 ggagccccag ccgcagcccg acctgggaag tgtcgacacc ggcgacacca gtgcgatcga
       61 cctgccctcc gccccgccgg gtggtggcgt ggcggccgtg aacgcgtcgt cggttcgccc
      121 tggggcggct ctgcgcgtac aggtgtcctt gcccgcaggg acggcggtgg cgatcatccg
      181 cggcgccgtg ggagacggct acctgcgcat cgcgcccacg cgcgaggcgc tgcgcgcccc
      241 aagtgggctg gatctgctgg ccggggcctt ggacgtgcaa cttgtcggcg tgaccttcga
      301 gctggcaagg ctctgcctgc cctacggcga ggccgcgtta ccggaggatg tgagcgagcg
      361 cgacatccgg ctgctgcact tcgacggtag gcgcccgcgc gacatcacgg tgcgggtgaa
      421 cgtgcgtggc gactcggtat gcggcgagac ggacggcttc tcgccgttcg cccttgccgt
      481 gcccgagatc tccgccgggc cgaccctgga gcgacttggg tcagctgatg cgatccgttc
      541 cgccagccag gtcgcaggct cgcgtttccg gtccggtgtg ccggttgtgt acgtggccag
      601 tggagagacc tacgcggacg tgctggcgtt agggcctgtg gcgaccccga gtggcggccc
      661 gatccttctg acccatcgcg accagctgcc tgaggcgacg cgtgagatgc tgctccgcct
      721 catgccgggg cgcatcgtca tcctgggcgg ccacggggcc atctccgctg tggtcgaaca
      781 ggcgctgcgg cagttcgccg ccgacggcgt gagccgcctg tcgggcttcg accggtatga
      841 gaccgcgagc gcgctactag cggcgtcctt cccggtggcg acaccggtcg tgttcatcgc
      901 gacgggtgag aacttccccg acgcgatcgc cggcggggcg gccgccgccc gtctgaacgc
      961 tcccgtggtg ctggtgcagc ccgacgccct cccggccgcg agcgctgcgg aactggcccg
     1021 gctccgtccg gagcgcatct acgtgctggg cggttccggt gccgtgagcg cgggggtcct
     1081 ggtccgtctg gccgagttcg cctccagctc ggtcgaacgg ctcgccggcg cggaccggtt
     1141 ccacacggcc gtgcgcatct cccacgagtt cttcccgaga ggcgcgtcga cggtgtacct
     1201 ggcggccggt gaagagttcg gggcggcgct cgccgctgtg cccgccgccg ctcgcgacag
     1261 tgcgccgatc ctgctgacgc ggtcgtcgca gatcccggcg gtggttcgcg acgagttgga
     1321 gcgcctgcgc ccggagcgca tcgtcatcct gggggctcga ggcgcgacgg cggcgatgct
     1381 gcgggaggac gtcgcggagt tgcagagccg ctggcgcgtc cagcggcgct aggacggccg
     1441 gtagtcggcc ggaccgaacc gctccagcac cttcagcagg gccatcaggt tgtgctgcac
     1501 gttgtccacg cggatgcggt tgtcgtacgc ctccgtgcgc acgccgccgc gcacgcgcgc
     1561 gcggtccgac acgtagtaca ggtccgcgtc gtcggcgaac tccagctgca tgacgctgcg
     1621 gatgccgcgc accagcgcca cgcggtagcg ctcggcccgg ccgtcgtcgc ccacctcgcg
     1681 ggccagccag aacgcgtcgg ccaggccctc caggtacacc cccgtggcgg acgcgtgcgg
     1741 cgggccgtat cccgggcggt cggggtcgta gaagcggccg cgcaggtccg ggtagggcgc
     1801 cgagtcccac tgctgcatgt ccagcagcca gtcgttcatc tcgaacacga acgtggccag
     1861 ctcgtcggag cgcgtgtgcc gccacgtctc cacgtaggcc tgcgtgtgcc acggcacgaa
     1921 cgcggggttg cggttggccg ggtccaggtg ccagccccgg tagaaccgga acgcgcgcat
     1981 gagccgttcc agggtcgcgg ggtctcggcg ctcggcgtag cggtgggccc agaagtacag
     2041 cgcctcgccg gggtagaagt tgtgctggtc gtcccggtcg gcgggacgga accaggtgcg
     2101 gaacgagccg tccggttcgg ccaggtgcac ggtggtggcc tccagggcgt cgatgaccgg
     2161 cgccagccgt gggcggtcgg gatgctcggc cagggcccgg ccggccagcg cgaccgcacc
     2221 gagcttcacc ttgtccgggc cctcctcgat cagcccgaga cccagttgct cggtgtagta
     2281 ggcgtcgagg ttgcgggcga cgttgcgccg ggtgagcgcc cagacgcgct cgtcgtcccg
     2341 ccaggcggcg tagcggccga gcgccgccgt ggccatccac tgccgcacgg tgttgtcgcc
     2401 ctgggactcc tcgccgcggc tggggaacca ctggtaggtc atgcggccgt cggggtgcac
     2461 ggcgcgggcg agccagtcgc ccaggcggcg cgccgtcgcc gcgaccgacg ccgcgtcgac
     2521 gtcctccgcc cgcacggtct cgttgccgcg gaacaggcgc cgggcccgca ccccgccgtc
     2581 gccgagcagc accaggaact gctcggcgtc gaacacgctg gcctcgacgc ccgcgaggtc
     2641 ttcgaggtcg cgcgcctcgg cgaacagctc cagcacgcgg gcgaacgagc ggttggtggc
     2701 gagcatccac gtggggctgt agcgcgccac ctcgccgcct cggcggagct ccaggccgcg
     2761 cacgccgcga tgcacgttgt ccagggcgcc gggcagcggc gagtccggcg gcg
//
LOCUS       DATMKC010000377         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_138041, whole genome shotgun sequence.
ACCESSION   DATMKC010000377 DATMKC010000000
VERSION     DATMKC010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138041"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VNU01_11065"
     CDS             <1..1403
                     /locus_tag="VNU01_11065"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HWH33197.1"
                     /translation="REPLRRALDVVRDAAVAAYDERAGALLKDPWSARDDYILVRLGE
                     LSRAEFLDRHAIGTLDDTRRTDVWTLLESQRHAMVMYTSCGWFFADVSGIETVYVLRS
                     AARVLGLLEELGAASPRAEVLDVLAEARSNKPGAGSGADIWRTQVAAAEVTPTRVAAH
                     LALTGLVRPLTEVEQVGGHAVHVADVRSEQRGRVGLATARFHVQSLATSRHHDFTVAA
                     VHLGGLDFHGVVAETAAHPGAHTLAVDALWDEFPTAPLARLLNLVTDVLDGEEFDLEQ
                     ALPEGRQDIVHAVFSELEGRFREVYERLYTDHRRILEMLTAAGYSLPRDLRAAAELTL
                     SGRLEQLLAGAPGEGGVDDEALGEVREILMLARSQGYRLDLTALERLLTGTVSSAARA
                     AAHTLDAHDIEAVERWMGLCAELAVDVDLSEAQEHAYEAAVRARTGRLNLEQREAAER
                     LGTRLGLSPLAWGGAP"
     gene            1488..>2270
                     /locus_tag="VNU01_11070"
     CDS             1488..>2270
                     /locus_tag="VNU01_11070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWH33198.1"
                     /translation="MRILLLSWEYPPRIHGGLGRHVHSLSVALARAGHEVHVVTRPHA
                     DAPLEEERDGVHVVRAPEPPPVLPPGDWVANALAVNTGLQTTATRLLNEIDVDVVHAH
                     DWLVAYAAGGVRITTGLPLVATIHATEYGRHQGYLPGPMNKFIHQAEWWLTYEARRVI
                     TCSAYMREQAERIFELPRGKVDTIPNGVTTEDFALLDGEELAALREAATPDGEHLVIY
                     AGRLEYEKGIQTLLHAVRDLRRGGTPVRLMVCGEGTYGGELRA"
BASE COUNT          300 a          826 c          860 g          284 t
ORIGIN      
        1 ggcgcgagcc gctgcggcgg gcgctggacg tggtgcgcga cgccgccgtg gccgcgtacg
       61 acgagcgcgc cggcgcgctg ctcaaggacc cgtggtccgc gcgcgacgac tacatcctgg
      121 tgcgcctggg cgagctcagc cgcgccgagt tcctggaccg ccacgccatc ggcacgctgg
      181 acgacacgcg ccgcacggac gtgtggacgc tgctggagag ccagcgccac gccatggtga
      241 tgtacaccag ctgcggctgg ttcttcgccg acgtgtccgg catcgagacc gtgtacgtgc
      301 tgcgctcggc ggcccgtgtc ctggggctct tggaagagct tggcgccgcg tcgccgcgcg
      361 cggaggtgct cgacgtgctg gcggaggcgc gcagcaacaa gccgggcgcg ggcagcggcg
      421 ccgacatctg gcgcacgcag gtggccgcgg cggaggtcac gcccacccgc gtcgccgcgc
      481 acctggcgct gaccgggctg gtgcgcccgc tgacggaggt cgagcaggtg ggcgggcacg
      541 ccgtgcacgt ggccgacgtg cgcagcgagc agcgcggccg cgtggggctg gccaccgcgc
      601 ggttccacgt gcagtcgctg gccaccagcc gccaccacga cttcaccgtc gcggcggtgc
      661 acctgggcgg cctggacttc cacggcgtgg tcgcggagac cgcggcgcac ccgggcgcgc
      721 acacgctggc ggtggacgcg ctgtgggacg agttccccac ggcgccgctg gcccgcctgc
      781 tgaacctggt caccgacgtg ctggacggcg aggagttcga cctcgagcag gcgctgccgg
      841 aaggccgcca ggacatcgtc catgccgtgt tcagcgagct ggagggccgc ttccgcgagg
      901 tgtacgagcg gctgtacacc gaccaccggc gcatcctgga gatgctgacg gcggccggct
      961 acagcctgcc gcgcgacctg cgggcggccg cggagctgac cctgtccggg cggctggagc
     1021 agctgctggc cggcgccccg ggcgaaggcg gcgtggacga cgaggcgctg ggcgaggtgc
     1081 gcgagatcct gatgctcgcg cggtcgcagg gctaccggct ggatctgacg gcgctggagc
     1141 ggctgctcac gggcaccgtg tcgtcggccg cgcgggccgc cgcgcacacg ctggacgcgc
     1201 acgacatcga ggccgtcgaa cgctggatgg gcctgtgcgc ggagctggcc gtcgacgtcg
     1261 acctgtcgga ggcgcaggag cacgcctacg aggcggcggt gcgggcccgc accggccggc
     1321 tgaacctgga gcagcgcgag gccgccgagc gcctgggcac ccgcctgggc ctgtctcccc
     1381 tggcctgggg cggcgccccc tgacccgtgg tgtgtcgact gagtcaacgc tggattgacc
     1441 gagtcgacac acccgggtgt gagcggctgg cctacactcc acggccgatg cgaatcctgc
     1501 ttctgagctg ggagtacccg ccgcgcattc acggcgggct gggccgccat gtccactcgc
     1561 tgtcggtggc gctcgcgcgc gccggccacg aggtccacgt ggtgacgcgg ccgcacgcgg
     1621 acgccccgct ggaggaggag cgcgacggcg tgcacgtcgt ccgcgccccc gagccgccgc
     1681 ccgtcctgcc gccgggcgac tgggtggcca acgccctggc cgtgaacacc ggcctgcaga
     1741 ccacggcgac gcgcctgctc aacgagatcg acgtcgacgt ggtgcacgcg cacgactggc
     1801 tggtggccta cgcggccggc ggcgtgcgca tcaccaccgg cctgcccctg gtcgccacca
     1861 tccacgccac cgagtacggc cgccaccagg gctacctgcc cgggcccatg aacaagttca
     1921 tccaccaggc cgagtggtgg ctgacctacg aggcgcgccg cgtgatcacc tgcagcgcct
     1981 acatgcgcga gcaggccgag cgcatcttcg agctgccccg cggcaaggtc gacaccatcc
     2041 ccaacggcgt gaccacggag gacttcgccc tcctggacgg cgaggagttg gccgccctgc
     2101 gcgaggcggc cacgcccgac ggcgagcacc tggtcatcta cgccggccgg ctggagtacg
     2161 agaagggcat ccagacgctg ctgcacgccg tgcgcgacct gcgccgcggc ggcacgccgg
     2221 tgcggctcat ggtctgcggc gagggcacct acggcggcga gctgcgcgcc
//
LOCUS       DATMKC010000378        13664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_5620, whole genome shotgun sequence.
ACCESSION   DATMKC010000378 DATMKC010000000
VERSION     DATMKC010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5620"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VNU01_11075"
     CDS             complement(<1..702)
                     /locus_tag="VNU01_11075"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWH33199.1"
                     /translation="MTATVKELLAERGRSFSFEFFPPKTDEGEQQLWRAISELADLEP
                     TFVSVTYGAGGSTRDRTIRITQRIARSSGITAAAHLTCVGSTVDELRAIVRQIGEAGI
                     RNVMALRGDPPGGPGQPWTATPGGLTHADELVALLKEEGDFCVGVAAHPEKHPEAPDL
                     ATDVKHLVGKFAAGADFAVTQFFFRAHDYFDLVQAVRAQGYDAPIIPGIMPVTNVAQI
                     ERMAALQGSDFPGDLA"
     gene            complement(790..1800)
                     /gene="ilvC"
                     /locus_tag="VNU01_11080"
     CDS             complement(790..1800)
                     /gene="ilvC"
                     /locus_tag="VNU01_11080"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF009940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWH33200.1"
                     /translation="MSDARMYYDDDASLEYLQGKTVAVIGYGSQGHAHARNLHESGVE
                     VVVGLYPGSKSAADAEAAGLEVLPVSDAAKRADIVMVLLPDTVQAAVYRNEIEPGLVD
                     GNALFFAHGFNIHFGQIVPPAGVDVCMVAPKGPGHLVRRTYTEGNGVPALFAVHQDAS
                     GKAREVALAYAKGIGAARAGVIETTFAEETETDLFGEQAVLCGGASSLVQKGFEVLVE
                     AGYQPEVAYFECLHELKLIVDLMYEGGLARMRYSISDTAEYGDYVSGPYVVDDGVKQR
                     MQEVLRRIQDGEFARDWILENQANQPSFRATRRRQAEHPIEKVGAKLRGMMSWLPKPT
                     NH"
     gene            complement(1803..2327)
                     /gene="ilvN"
                     /locus_tag="VNU01_11085"
     CDS             complement(1803..2327)
                     /gene="ilvN"
                     /locus_tag="VNU01_11085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWH33201.1"
                     /translation="MSKHTLSVLVEDRPGVLARVAGLFSRRGFNIESLAVGPTEVDGV
                     SRMTIRVDAADTALEQVTKQLNKLIHVLKIVELEDNAAVERELQMVKVAVNPGERGEI
                     IEIADVFRAKVVDVDTDSITIEATGSPDKLEAMVRLLTPYGIRELVRSGMIAVGRGSK
                     SITEGRALRLERTA"
     gene            complement(2330..4159)
                     /locus_tag="VNU01_11090"
     CDS             complement(2330..4159)
                     /locus_tag="VNU01_11090"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWH33202.1"
                     /translation="MAGGTAHEPRTRGAVMEITGAQAVIRSLELEGVDVVFGHPGGAI
                     LPAYDPILDSSIRHILARHEQGAGHMAEGYAWATGRTGVAIATSGPGGTNLVTALADA
                     HMDSVPMVAITGQVPTGAVGGDAFQEADMTGITMPVTKHNEMITDPDRIPGAIKEAFH
                     IASTGRPGPVLVDIPKDILNATCHFAWPDRVNLPGYKPTVRGHGKMVREAAREILLAK
                     RPVIYAGGGIIKAGAHEELRKLAELANLPVVTTLMARGAFPDSHPQHLGMPGMHGHWA
                     AVTSMQQADLLIALGARFDDRVTGNLATFAPHARIIHVDVDPAEIGKNREAHVPIVGD
                     CRVVLQQITDVLTEALKDYRYDLSEWWTTLDAWRRDHPLRAEQPADGPLKPQTVVRAI
                     SDKLGGEGIVVTGVGQHQMWAAQHIRFEKPRSWINSGGLGTMGFCVPAAIGAKVGMPD
                     KTVIAVDGDGSFQMTLQELATAKTEGVPVIFCVLNNAGLGMVRQWQQLFYDERYSQVD
                     MPADVPDLVKLADAYGCVGFRCDKAEDVESTLDKALAVGEVPVIVDFRIDQTEKVFPM
                     VPAGASNDDIIESAEEWYARVDAAKQASSDGQLPENMREGNVV"
     gene            complement(4725..5417)
                     /locus_tag="VNU01_11095"
     CDS             complement(4725..5417)
                     /locus_tag="VNU01_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH33203.1"
                     /translation="MSTFGSSAGEQGDDRPEGSTPIRVVIAEDHGVVADGVATMLSFE
                     ADMEVIEIVTSGEDLIDSVEKHRPDVALVDVNLIGMDGLAAVREIEARGIPTRTMALT
                     MFTDHDTVTRAVAAGVAGYLPKNVRREELVQAVRAVAAGKGFLHPDVTRPFLERVGPM
                     AMHAGIQPLSKREQEVLEQLATGRSTKEIADALGLGDETVKSHLSRIYQKLKATDRVQ
                     AVVTAMRHGLVR"
     gene            complement(5414..7084)
                     /locus_tag="VNU01_11100"
     CDS             complement(5414..7084)
                     /locus_tag="VNU01_11100"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWH33204.1"
                     /translation="METGVPPQRETKVHPAFGVYVALLAGAALLFFPAGLMEAARDPW
                     ATLLWILFIAAANLLTIPASPKWSIDASLGAPVSVSAAVVLSPPLAIVVNFLGLVNER
                     EWRGGTSIWMVLFNRSQLALSAGAAAFAADMVPWSNIVASMLAVIVYTVVNTAAVSFQ
                     VLLRRKVQFLEAAKGSAAPFPHFMLDFGLVTLLSVLIVIGHEQVGPLAVLLLALPLWL
                     GYSALRSAREAEDRAEQLAARVRELETLHALGTELLTARDVERVRIIALGALRSALDT
                     DLVDVSSGGEVREGLVPVSVPGAEPFTIGVPPGLSEQARAGVEAIAGLLGNALQRVRL
                     ERELGAVERARTALSGQILEEGTRERNRIALQVHDEVLPYLAAAEIQADNVRSSLSRD
                     DRARADELAKATQQLVHDGIHRLREVIAALRRQIVTPGGLREALLGALEEIRLSAGVE
                     GSLSAPDPLPPLPLAVEILVLETVRGCLANVANHAKADTVEVRVEVVEGRIRLEICDD
                     GAGFDPAAVPPGHHGLGLMSQRVGLARGTFRVDSEQGAGTTVHVEVPV"
     gene            complement(7816..8334)
                     /locus_tag="VNU01_11105"
     CDS             complement(7816..8334)
                     /locus_tag="VNU01_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWH33205.1"
                     /translation="MQRPRTVDADIVTDLATRTPVLARRTLGLHTERAFHVTDVTEDC
                     QSLLEASGVQDGTLTVYSEHTTCAVKINERETCFLEDFRLFMEHLVPADAYYRHDDLE
                     LRAPETLAGAPEEEPINGHAHIKQMLLGASSETVPVVDGRLALGRWQRIMFIELDQAR
                     PRQIQLQVQGWR"
     gene            complement(8520..9623)
                     /locus_tag="VNU01_11110"
     CDS             complement(8520..9623)
                     /locus_tag="VNU01_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWH33206.1"
                     /translation="MPPPAAAGPTALEAARAPVEAVLRAYLAQASARLAALDPALADV
                     ATPAVELALSGGKRLRPAFVHWGARAAGAALEGELGEAVHTAGAAVELLHTFALVHDD
                     VMDRAQWRRGRPALHRAMAEWHAAEGLVGDAGWFGIGAAILAGDIVFVWADELFDRAA
                     AAVAEPAAAREVFTRLRVEVMAGQYLDLRLAGLPRPTAEEALRVALLKSGRYTVTRPL
                     QLGAALGGAGSSRPGDALGSALSAYGDALGVAFQLRDDVLGLYGDPAATGKEALGDLR
                     EGKRTLLVLRALELAGDRDRAAVAAALGDPALDHDGADRVRRIVRDTGALAAVEREME
                     QRLAEAVQALAAVPEPARSALAELARDSVHRLA"
     gene            complement(9626..11089)
                     /gene="gatB"
                     /locus_tag="VNU01_11115"
     CDS             complement(9626..11089)
                     /gene="gatB"
                     /locus_tag="VNU01_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWH33207.1"
                     /translation="MSTVTETTYEAVIGLEVHVELDTRTKMFCSCAIDFGGEPNTRTC
                     PVCLGQPGSLPVVNREAVAAAIKLGLALGCRIAPTSEFHRKNYFYPDMPKDYQISQYD
                     VPICLGGSLRVDTSEGEKVVGITRVHMEEDTGKSLHVGTSGRIHGADYSLVDYNRAGI
                     PLLEIVSEPDVRSAEEAKAYLTELRAIVLALGISDAKLEEGSMRCDANISIRPAGQAE
                     YGTRVEVKNMNSVRSLGRAIEYEIARQIDMVEAGERIVQETRHWNEDAGTTSTLRSKE
                     EATDYRYFPDPDLVAVAPDSAWIEQIRAGLPELPAQARTRLRDELGLPADRAGTVVGN
                     RLVGRLDEAVAAGASAPEAANWLVNEITGWAVERGMEPAEAPVTGVQLAELVALVGDG
                     TLSTKLAREVLTDVLEGKGSPRGIVAERGLEQISDTGALEAIVDQVIAEQADAAQRVR
                     DGNAKAIGALVGAVMKASQGKANPAMVNELLRARLLG"
     gene            complement(11086..12579)
                     /gene="gatA"
                     /locus_tag="VNU01_11120"
     CDS             complement(11086..12579)
                     /gene="gatA"
                     /locus_tag="VNU01_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWH33208.1"
                     /translation="MSELVTRTAAELRDLLAAGEVSAREVAQAHLDRIRATDGRVKAF
                     LKVLDEQALAHAGDVDRRRAAGEPLGPLAGVPLALKDILATKGIETTCASRILETYRP
                     PYDATVVKRLRQADVVVLGKTNMDEFAMGSSTENSAFSKTYNPWDLARVPGGSSGGSS
                     AAVAAFDAPLGIGTDTGGSIRQPAAFCGIVGVKPTYGLVSRFGLIAFASSLDQVGPFA
                     RSVTDAALLLDAIAGHDPADSTSIPEAKPDFLAGIDKGVQGLRVGVVREFMAEGMEPG
                     VRARVDEAVARLEKLGATVVDVSLPHASYGVAAYYLIAPSEASSNLSRYDGVRYGLRV
                     PGETAEEMMANTRAAGFGPEVKRRIMIGTYALSAGYYDAYYAQAQRVRTLIIRDFAEA
                     YAQCDVLVGPTTPSTAFTVGEKVDDPLAMYLNDVYTIPSNLAGAPALSLPIGLDEHHL
                     PVGLQVMAPVLGEPAMFRVARALEADLAFDPTPRGANALETPGGAAA"
     gene            complement(12576..12872)
                     /gene="gatC"
                     /locus_tag="VNU01_11125"
     CDS             complement(12576..12872)
                     /gene="gatC"
                     /locus_tag="VNU01_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWH33209.1"
                     /translation="MALSTDDVRHVARLARLALSDEEVEALRSQLSDILAYAEQVGEA
                     AGGDVPPTSHAYPLANVFRADEPHESLSPEEAISTAPEAEDGRFRVPKIVGGEA"
     gene            complement(12968..>13664)
                     /locus_tag="VNU01_11130"
     CDS             complement(12968..>13664)
                     /locus_tag="VNU01_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWH33210.1"
                     /translation="VEPLAHRRGAMDIEGLGYETAKVLLDKALVANLADVYRLADRRA
                     ELLELERFGERKVDNLLAGIEASKARPFEHLLVGLNIRHVGGTVARLLARHFGSMEGL
                     RAATEDEIAGVTGVGPIIAAAVRQWFDNDRNAKLVDDLAGLGVRMDTDVTKVGDSLAG
                     KTFVLTGGLEGFTRDEAKQAIEDRGGKVAGSVSKKTDYVVVGADPGSKAAKAAELGVP
                     ALDEAAFIALLDA"
BASE COUNT         1858 a         4964 c         4807 g         2035 t
ORIGIN      
        1 ggccaggtca ccggggaagt ccgagccttg gagggccgcc atgcgctcga tctgcgcgac
       61 gttcgtcacc ggcatgatcc cggggatgat cggcgcgtcg taaccctggg cccgcaccgc
      121 ctggaccagg tcgaagtagt catgcgcgcg gaagaagaac tgcgtcaccg cgaagtccgc
      181 gccggcggcg aacttgccca ccagatgctt cacgtcggtg gcgagatccg gggcctccgg
      241 atgcttctcc gggtgggccg ccacgccgac gcagaagtcg ccctcctcct tgagcagcgc
      301 gaccagctcg tcggcgtgcg tgagcccgcc aggcgtggcg gtccacggct gccccggacc
      361 gcccggcggg tcaccgcgca gggccatgac gttgcggatg ccggcctcac cgatctggcg
      421 cacgatcgcg cgcagctcgt cgacggtcga gccgacgcag gtgagatgcg cagccgccgt
      481 gatgccgctg gaccgcgcga tgcgctgggt gatgcggatg gtgcggtcac gcgtggagcc
      541 gccggcgccg taggtcaccg agacgaacgt gggctccagg tcggcgagct cggagatcgc
      601 gcgccacagc tgctgctcgc cctcgtcggt cttcggcggg aagaactcga acgagaacga
      661 tcggccgcgc tcggccagca gctccttgac ggtggcggtc atcagcaggc gtccacaact
      721 tcccccctca tatgggtgcg gttccggagc catggctccg aaaccgcacc caacgagaga
      781 agcggtcggt cagtggttcg tgggcttggg cagccagctc atcatcccgc gcaacttcgc
      841 gccgaccttc tcgatcgggt gctcggcctg gcggcggcgc gtggcccgga agctcggctg
      901 gttcgcctgg ttctccagga tccagtcgcg ggcgaactcg ccgtcctgga tgcgccgcag
      961 cacctcctgc atgcgctgct tcacgccgtc gtccaccacg tacggtcccg acacgtagtc
     1021 gccgtactcg gcggtgtccg agatcgagta gcgcatgcga gccaggccgc cctcgtacat
     1081 gaggtcgacg atcagcttca gctcgtgcag gcactcgaag taggcgacct ccggctggta
     1141 gccggcctcc accaggacct cgaagccctt ctgcaccagg ctggaggcgc cgccgcagag
     1201 caccgcctgc tcgccgaaca ggtcggtctc ggtctcctcg gcgaacgtcg tctcgatgac
     1261 gccggcccgc gcggcgccga tgcccttggc gtaggcgagc gcgacctcgc gcgccttgcc
     1321 ggaggcgtcc tggtggaccg cgaacagcgc gggcacgccg ttgccctccg tgtaggtgcg
     1381 gcgcaccagg tggccggggc ccttcggcgc gaccatgcac acgtccacgc ccgcgggcgg
     1441 cacgatctgg ccgaagtgaa tgttgaagcc gtgcgcgaag aacagcgcgt tgccgtccac
     1501 cagccccggc tcgatctcgt tgcggtacac cgccgcctgg acggtgtccg gcagcaggac
     1561 catcacgatg tcggcgcgct tcgcggcgtc gctgacgggc aggacctcca ggcccgccgc
     1621 ctccgcgtcc gcggcgctct tggagcccgg gtacaggccg acgacgacct cgaccccgga
     1681 ctcgtggagg ttgcgggcgt gggcgtggcc ctgcgacccg tagccgatga cggcgacggt
     1741 cttgccctgg aggtattcca gggaggcgtc gtcgtcgtag tacatgcgtg cgtcggacat
     1801 tgctacgcgg tcctttccag acggagggcg cgcccctcgg tgatgctctt gctgccgcgg
     1861 cccacggcga tcatgccgga ccgcaccagc tcacggatgc cgtacggggt gagcaggcgc
     1921 accatcgcct cgagcttgtc cggcgagccg gtcgcctcga tggtgatgga gtcggtgtcc
     1981 acgtccacga ccttggcgcg gaacacgtcg gcgatctcga tgatctcgcc gcgctcaccc
     2041 gggttgaccg ccaccttcac catctggagc tcgcgctcca cggcggcgtt gtcctccagc
     2101 tccacgatct tcagcacgtg gatgagcttg ttgagctgct tggtgacctg ctccagggcg
     2161 gtgtcggcgg cgtccacgcg gatggtcatg cgcgacaccc catccacctc cgtggggccg
     2221 accgccagcg actcgatgtt gaagccgcgg cggctgaaca ggcccgcgac gcgggccagg
     2281 acgcccgggc ggtcctccac cagcacggac agcgtgtgct tggacatggc tagaccacgt
     2341 tcccttcgcg catgttctcg gggagctgcc cgtccgacga cgcctgcttg gcggcgtcca
     2401 cgcgggcgta ccactcctcg gccgactcga tgatgtcgtc gttgctggcg ccggccggca
     2461 ccatcgggaa aaccttctcg gtctggtcga tgcggaagtc cacgatcacc gggacctcgc
     2521 ccaccgccag cgccttgtcc agcgtggatt ccacgtcctc cgccttgtcg cagcggaagc
     2581 ccacgcagcc gtaggcgtcg gccagcttga ccaggtccgg cacgtccgcc ggcatgtcca
     2641 cctggctgta gcgctcgtcg tagaacagct gctgccactg gcgcaccatg ccgaggccgg
     2701 cgttgttcag cacgcagaag atcaccggca cgccctcggt cttggcggtg gccagctcct
     2761 gcagggtcat ctggaagctg ccgtcgccgt cgacggcgat cacggtcttg tccggcatgc
     2821 cgaccttggc gccgatcgcc gccggcacgc agaagcccat ggtccccagg ccgccggagt
     2881 tgatccagct gcgcggcttc tcgaagcgga tgtgctgggc ggcccacatc tggtgctggc
     2941 ccacgcccgt caccacgatg ccctcgccgc ccagcttgtc gctgatggcg cgcaccacgg
     3001 tctgcggctt gagcgggccg tcggccggct gctcggcgcg cagcgggtgg tcgcggcgcc
     3061 acgcgtccag cgtcgtccac cactccgaca ggtcgtagcg gtagtccttc agcgcctcgg
     3121 tcagcacgtc ggtgatctgc tggagcacca cgcggcagtc gcccacgatc ggcacgtgcg
     3181 cctcgcggtt cttgccgatc tccgccggat ccacgtcgac gtggatgatc cgcgcgtgcg
     3241 gggcgaaggt cgccaggttg ccggtgacgc ggtcgtcgaa gcgcgcgccc agggcgatga
     3301 gcaggtccgc ctgctgcatc gacgtgacgg cggcccagtg cccgtgcatg cccggcatgc
     3361 ccaggtgctg cgggtggctg tcggggaacg cgccccgcgc catgagcgtg gtcaccaccg
     3421 gcaggttggc gagttccgcc agcttgcgca gctcctcgtg cgcgcccgcc ttgatgatgc
     3481 cgccgccggc gtagatgacc gggcgcttgg ccagcaggat ctcgcgggcg gcctcgcgca
     3541 ccatcttccc gtggccgcgc accgtcggct tgtacccggg caggttcacg cggtccggcc
     3601 acgcgaagtg gcaggtcgcg ttgaggatgt ccttggggat gtccaccagc accgggcccg
     3661 gccggccggt ggaggcgatg tggaacgcct ccttgatcgc gccggggatg cggtcggggt
     3721 cggtgatcat ctcgttgtgc ttggtgaccg gcatggtgat gccggtcatg tccgcctcct
     3781 ggaaggcgtc accgccgacc gcgccggtcg gcacctggcc cgtgatcgcg accatcggca
     3841 cggagtccat gtgcgcgtcg gccagtgcgg tgacgaggtt ggtgccaccg gggcccgacg
     3901 tcgcgatggc cacgcccgtg cggcccgtcg cccaggcgta gccctccgcc atgtgcccgg
     3961 cgccctgctc gtggcgggcc aggatgtggc ggatggacga gtccaggatc gggtcgtacg
     4021 ccggcaggat ggcgccaccg ggatgcccga agacgacgtc gacgccttcc agctccagtg
     4081 acctgatgac ggcctgcgct ccggtgatct ccatgacggc ccctcgggtc cgtggttcgt
     4141 gtgctgtgcc gcccgccacc ctcggcgggc aatgaaaaga cccctcgccg ggatggcaga
     4201 gaggtcgcgc ctggtgtgct gcggccgtgc cgcgggtcag accaggcgcg gtgagaggga
     4261 tacgagcacg ccacgcgtct ccatcatgcc gtgcacctcc cctctcgcgc tggtccgtcg
     4321 aggcggttcg cctcgccgtc gggtgctacc cacggttttg acgcgccgca tcgcgctgac
     4381 cgctgcggcg ccatcgccgc tcgctagtgt ggtcgcgtgt ccgacgaact gtcaactcga
     4441 tagcgagaag gtttcgtcgg cgggcgcagg atcgcgccag cggagtgccc gggatcgccg
     4501 ttcgcgcggc ctaccagatg aaatgcccca tatagcctgt ccgccaggac aagggttttt
     4561 gcgggtcggt gggttcccga cagggatcat gggggcacca acaggtccta catcaaggca
     4621 ccatgcggcg ggcgggttcc cgccccctcc cgccgcagac ggtgtcgcac acagagagcc
     4681 ccgactcccc ccggtcgggg ctctctatct ttcttggggc gaggctatct gacgaggccg
     4741 tggcgcatgg cggtgacgac ggcctggacg cggtcggtcg ccttcagctt ctggtagatc
     4801 cgggacaggt ggctcttcac ggtctcgtcg ccgaggccga gcgcgtcggc gatctccttg
     4861 gtggagcgcc cggtcgccag ctgctccagc acctcctgct cgcgcttgga cagcggctgg
     4921 atgccggcgt gcatcgccat cgggcccacg cgctccagga acggccgggt cacgtccggg
     4981 tgcaggaagc ccttgccggc ggcgaccgcc cgcaccgcct gcaccagctc ctcgcggcgc
     5041 acgttcttcg gcaggtagcc ggccacgccc gcggccaccg cgcgggtcac cgtgtcgtgg
     5101 tcggtgaaca tggtgagcgc catggtccgg gtcgggatgc cgcgcgcctc gatctcgcgc
     5161 acggcggcca ggccgtccat gccgatcagg ttcacgtcga ccagggcgac gtcggggcgg
     5221 tgcttctcca ccgagtcgat caggtcctcg ccggacgtga cgatctcgat gacctccatg
     5281 tccgcctcga acgacagcat ggtggccacg ccgtcggcga cgacgccgtg gtcctcggcg
     5341 atgacgacgc ggatgggagt ggacccctcc gggcggtcgt cgccctgctc gcccgcggac
     5401 gatccgaacg tgctcacact ggcacctcca cgtgcaccgt tgtccccgcg ccctgctcgc
     5461 tgtccacacg gaacgtgccg cgagcaaggc ccacacgctg cgacatcagc ccgagcccgt
     5521 ggtggccggg aggcacggcg gcgggatcga acccagcgcc gtcatcacag atctccaagc
     5581 ggatccgccc ctccaccacc tccacgcgca cctcgacggt atccgccttt gcgtgatttg
     5641 ccacattcgc caggcagccc cgcaccgtct cgagcaccag gatctccacc gccaagggta
     5701 acggaggcaa ggggtccgga gcgctgaggc tgccctcgac gccggcggac agccggatct
     5761 cctccaacgc gcccagcagc gcctcccgca ggccgccggg cgtgacgatc tggcgccgca
     5821 gcgccgcgat gacctcgcgc aggcggtgga tgccgtcgtg gaccagctgc tgcgtcgcct
     5881 tggcgagctc gtccgcgcgg gcacggtcgt cccgggacag cgacgagcgc acgttgtccg
     5941 cctggatctc cgcggccgcc aggtacggca gcacctcgtc gtgcacctgc agggcgatgc
     6001 ggttccgctc gcgcgtgccc tcctccagga tctggcccga cagggcggtg cgggcgcgct
     6061 ccacggcgcc gagctcgcgc tccaggcgca cgcgctgcag ggcgttgccc agcagcccgg
     6121 cgatggcctc cacgccggcg cgcgcctgct cgctcaggcc cggcgggacg ccgatggtga
     6181 acggctcggc gcccggcacg ctcaccggca cgagcccctc gcgcacctcg ccgcccgagg
     6241 agacgtccac caggtcggtg tccagcgcgg agcgcagcgc gccgagcgcg atgatgcgca
     6301 cccgttcgac gtcccgcgcg gtcagcagct ccgtgccgag cgcgtggagc gtctccagct
     6361 cccgcacgcg cgccgccagc tgctcggcgc ggtcctccgc ctcacgggcg gagcgcagcg
     6421 cgctgtagcc gagccacaac ggcagggcga gcagcagcac cgcaagcggc cccacctgct
     6481 cgtggccgat gacgatcagg accgacagca gcgtgaccag gccgaagtcc agcatgaagt
     6541 gcgggaaggg cgccgccgag cccttggccg cctccaggaa ctgcaccttg cgccgcagca
     6601 gcacctggaa ggagaccgcc gcggtgttga ccacggtgta gacgatcacc gcgagcatcg
     6661 aggcgacgat gttgctccac ggcaccatgt cggccgcgaa cgccgccgcg cccgccgaca
     6721 gcgcgagctg gctgcggttg aacaggacca tccagatgga ggtgccgccg cgccattccc
     6781 gctcgttgac caagcccagg aagttcacga cgatcgcgag cggcggcgac aggaccaccg
     6841 cggccgacac gctgaccggg gcgccgaggc tggcgtcgat gctccacttg ggcgaggcgg
     6901 ggatggtcag caggttcgcc gcggcgatga acaggatcca cagcagggtc gcccacgggt
     6961 cccgtgcggc ctccatgagg ccggccggga agaacagcag cgccgcccct gcaagaaggg
     7021 cgacgtacac gccgaaggcc gggtggacct tcgtctcccg ttgaggggga actccggttt
     7081 ccacccggcc tcctgaggct ccggccgcgc tcgtcaaccc ccgagcgcgt gccggcaaac
     7141 cttaccgcac cgacaggtgc gggagtgcgc tcttaccagg ccgtcttgtt ggccagaccg
     7201 ctggcgacca gggccgcaag cgtggtgagg gcggcgatga cgcggggcga gaacttcttc
     7261 atgatgatcc tcctgatgat gttttgcgtt tcgggcatgg acaaaacact gtctggctga
     7321 acgcccgccc tggttcgctc acgcgaaccg atcgaggagc cagacgagga ggaggcctcc
     7381 gctccaagac cagtgatacc acgccgatac gtgacgcccc aagcacggtg cgcaatctcg
     7441 gagagaaaat gcccgatttg ggagccttgg agtaacgaaa tcgttactta ccgtgatatc
     7501 ggccctggtc tcgtgcggta gccgcgcact cgcgtgcgga gccgccccac tcccctcgga
     7561 tacctgtcgg cgacaaggcc actgccgcaa ccactttgcc gacatgtctg ctcaccggcc
     7621 ccacgagggg gacgggtgcc gtccgtggct gcgacgcgcc gcacctgcag gctccgaggg
     7681 gagccccggg gcgctccggc gccggccgcc caggcgaacc cagacggtcg cgacgcctcg
     7741 cgtcgagtgg cacaagccta aacagcagtc acacaagtcg tcaacagttg acttcggcga
     7801 cgctgaggcg gcggttcagc gccagccctg cacctgcagc tggatctgcc gggggcgcgc
     7861 ctggtccagc tcgatgaaca tgatccgctg ccaccggccc agggccagcc ggccgtcgac
     7921 gaccggcacc gtctcggagg acgcgcccag cagcatctgc ttgatgtggg cgtggccgtt
     7981 gatcggctcc tcctccggcg cgccggccag ggtctccggc gcgcgcagct ccaggtcgtc
     8041 gtggcggtag taggcgtccg ccggcaccag gtgctccatg aacaggcgga agtcctccag
     8101 gaagcacgtc tcccgctcgt tgatcttgac cgcgcaggtg gtgtgctcgc tgtagaccgt
     8161 gagcgtgccg tcctggacgc cgctggcctc cagcagggac tggcagtcct cggtcacgtc
     8221 ggtgacgtgg aacgcgcgct cggtgtgcag gccaagggtc cggcgggcca ggacgggcgt
     8281 gcgggtggcg aggtcggtga cgatgtcggc atcgaccgtg cgcggacgct gcatggtggg
     8341 ggcctccggc gacggagcgg caccgcggac accggcgcga acgccgtcga cagacgagtc
     8401 gttctggatc ggctgcactc tactcgcttt cgtcacggat gccggatagg ggcccgacgt
     8461 gcgattctcc atgctgtcag cattcgccag cctcggccat tcggccgggc cgccggcgct
     8521 catgcgagcc ggtggaccga gtcccgcgcc agctcggcca gggcggacct ggccggctcg
     8581 gggaccgcgg ccagagcctg caccgcctcc gcgaggcgct gctccatctc gcgctccacc
     8641 gccgcgagcg ccccggtgtc gcgcacgatc cgccgcacgc ggtcggcgcc gtcgtgatcc
     8701 agcgccgggt cgcccagcgc cgccgccacc gcggcccgat cgcggtcgcc cgccagctcc
     8761 agggcgcgca ggaccagcag cgtgcgcttg ccctcgcgca ggtcgcccag cgcctccttg
     8821 cccgtcgcgg ccgggtcgcc gtacaggccc agcacgtcgt cgcgcagctg gaacgcgacg
     8881 cccagcgcat cgccataggc gctcagggcg ctgcccagcg catccccggg ccgtgaggag
     8941 cccgcgccgc ccagcgccgc gcccagctgc agcggccgcg tgaccgtgta gcggcccgac
     9001 ttgagcagcg ccacgcgcag ggcctcctcc gcggtgggcc gcggcaggcc cgccagcctc
     9061 aggtccaggt attggccggc catgacctcc acgcgcagcc tcgtgaagac ctcgcgggcc
     9121 gccgcgggct ccgcgaccgc ggcggccgcg cggtcgaaca gctcgtcggc ccagacgaac
     9181 acgatgtcac cggccaggat ggccgcgccg atcccgaacc agccggcgtc gccgaccagc
     9241 ccctcggccg cgtgccactc cgccatcgcg cggtgcagcg cgggccgccc gcgccgccac
     9301 tgcgcccggt ccatgacgtc gtcgtgcacc agcgcgaacg tgtgcagcag ctccacggcc
     9361 gccccggccg tgtgcacggc ctcgccgagt tcgccctcca gcgccgcgcc ggcggcccgc
     9421 gcgccccagt ggacgaacgc cggccgcagg cgcttgccgc ccgacagcgc gagctcgacc
     9481 gccggggtgg cgacgtcggc cagcgcgggg tcgagcgcgg ccaggcgcgc ggaggcctgc
     9541 gccaggtagg cccgcagcac cgcctccacg ggagcccgcg ccgcctccag ggcggtcggg
     9601 ccggcggctg cgggcggcgg cacgctcagc ccaggagcct ggcgcgcagc agctcgttca
     9661 ccatcgcggg attggccttg ccctgcgacg ccttcatcac ggcgccgacc agcgcgccga
     9721 tggccttggc gttgccgtcg cgcacgcgct gggcggcgtc ggcctgctcg gcgatcacct
     9781 ggtcgacgat ggcctccagg gcgccggtgt cgctgatctg ctccaggccg cgctcggcga
     9841 cgatgccccg tggggagccc ttgccctcca acacgtccgt gagcacctcg cgcgcgagct
     9901 tggtggacag cgtgccgtcg ccgaccagtg ccaccagctc cgccagctgg acgccggtga
     9961 cgggcgcctc ggcgggctcc atcccgcgct cgaccgccca gccggtgatc tcgttcacca
    10021 gccagttggc ggcctcgggc gcggacgcgc cggcggccac cgcctcgtcc agcctgccga
    10081 ccagcctgtt gccgaccacg gtccccgcgc ggtccgcggg cagccccagc tcgtcgcgca
    10141 gccgggtgcg ggcctgggcg ggcagttccg gcaggccggc gcggatctgc tcgatccacg
    10201 cggaatcggg cgcgaccgcc accaggtccg ggtcggggaa gtagcggtag tcggtggcct
    10261 cctccttgct gcgcaaggtg gaggtggtgc cggcgtcctc gttccagtgg cgcgtctcct
    10321 gcacgatgcg ctcgccggct tccaccatgt cgatctggcg ggcgatctcg tactcgatcg
    10381 cccggcccag gctgcgcacc gagttcatgt tcttcacctc gacgcgcgtg ccgtactcgg
    10441 cctgcccggc cgggcggatc gagatgttgg cgtcgcagcg catcgagccc tcttcgagct
    10501 tggcgtcgct gatgcccagg gccagcacga tggcgcgcag ctcggtgagg tacgccttgg
    10561 cctcctcggc gctgcgcacg tcgggctcgg acacgatctc cagcagcggg atcccggcgc
    10621 ggttgtagtc gaccagggag tagtcggcgc cgtggatgcg ccccgacgtg cccacgtgga
    10681 ggctcttgcc ggtgtcctcc tccatgtgca cgcgcgtgat gccgacgacc ttctcgcctt
    10741 cagaggtgtc cacccgcagg gagccgccca ggcagatcgg cacgtcgtac tggctgatct
    10801 gatagtcctt gggcatgtcg ggatagaagt agttcttgcg gtggaactcg ctggtcgggg
    10861 cgatgcggca gcccagggcc aggcccagct tgatcgccgc ggccaccgcc tcgcggttga
    10921 ccaccggcag gctgccgggc tggcccaggc acaccgggca ggtgcgcgtg ttcggctcgc
    10981 cgccgaagtc gatcgcgcac gagcagaaca tcttggtgcg cgtgtccagc tccacgtgca
    11041 cctcgaggcc gatcacggcc tcgtacgtcg tctcggtcac ggtgctcatg ccgcggcgcc
    11101 tcccggcgtc tccagcgcgt tcgcgcctcg gggggtgggg tcgaaggcga ggtcggcctc
    11161 cagcgcgcgg gccacgcgga acatcgccgg ctcgcccagc acgggcgcca tgacctgcag
    11221 gcccaccggc aggtgatgct cgtccaggcc gatcggcagg ctgagcgccg gggcgccggc
    11281 caggttggac gggatcgtgt agacgtcgtt caggtacatg gccagcgggt cgtcgacctt
    11341 ctcgcccacc gtgaacgcgg tgctgggcgt cgtcgggccg accagcacgt cgcactgcgc
    11401 gtacgcctcg gcgaagtcgc ggatgatcag cgtgcggacg cgctgcgcct gggcgtagta
    11461 ggcgtcgtag tagccggccg acagcgcgta cgtgccgatc atgatgcggc gcttcacctc
    11521 cgggccgaag cccgcggcgc gcgtgttcgc catcatctcc tcggccgtct cgccgggcac
    11581 gcgcaggccg tagcgcaccc cgtcgtagcg cgacaggttg ctggaggcct cgctcggcgc
    11641 gatcaggtag taggcggcca cgccgtagga ggcgtgcggc aggctcacgt cgaccaccgt
    11701 ggcgcccagc ttctccaggc gcgcgacggc ctcgtccacg cgggctcgca cgcccggctc
    11761 catgccctcg gccatgaact cgcgcaccac gccgacgcgc aggccctgca cgcccttgtc
    11821 gatgccggcg aggaagtccg gcttggcctc ggggatcgac gtggagtcgg cggggtcgtg
    11881 gccggcgatg gcgtccagca gcagcgccgc gtcggtgacg gagcgcgcga acgggccgac
    11941 ctggtccagg gaggacgcga acgcgatcag gccgaagcgg ctgaccagcc cgtaggtcgg
    12001 cttcacgccg acgatgccgc agaacgcggc cggctggcgg atggacccgc cggtgtccgt
    12061 gccgatgccc agcggggcgt cgaacgccgc gaccgcggcg ctggacccgc cggaggagcc
    12121 gcccggcacg cgggcgaggt cccaggggtt gtaggtcttg gagaacgccg agttctccgt
    12181 ggacgagccc atcgcgaact cgtccatgtt ggtcttgccg agcaccacga cgtcggcctg
    12241 gcgcagccgc ttgaccacgg tggcgtcgta ggggggtcgg taggtctcca ggatgcgcga
    12301 cgcgcaggtg gtctcgatgc ccttggtggc gaggatgtcc ttgagggcca gcggcacgcc
    12361 ggccagcggc ccgagcggct cgcccgccgc gcggcggcgg tcgacgtcgc cggcgtgggc
    12421 cagcgcctgc tcgtccagca ccttcaggaa cgccttgacc cggccgtcgg tggcgcggat
    12481 gcggtccagg tgcgcctggg cgacctcgcg cgcgctgacc tcgccggcgg ccaggaggtc
    12541 gcgcagctcg gcggcggtcc tggtgacgag ctcgctcacg cctcgccacc cactatcttg
    12601 ggcacgcgga agcggccgtc ctcggcctcc ggggcggtgg agatcgcctc ctcgggcgac
    12661 aaggactcgt gcggctcgtc cgcccggaag acgttcgcca aggggtaggc gtggctggtg
    12721 ggcggcacgt cgccgcccgc cgcctccccc acctgctcgg cgtaggcgag gatgtccgac
    12781 agctgcgagc gcagcgcctc gacctcctcg tcggacagcg ccaggcgcgc gaggcgcgcc
    12841 acgtgccgga cgtcgtcagt gctcagtgcc atagcgccca aagctagcag cgctccccgg
    12901 gggtccccat tcgaacgggt gtggggactg cgggataccg cggtgcccgc acccgttcaa
    12961 acggggatca ggcgtcgagg agcgcgatga aggcggcttc gtccagggcg gggacgccga
    13021 gttcggcggc cttggcggcc ttgctgcccg ggtcggcgcc caccaccacg tagtcggtct
    13081 tcttggacac gctgccggcg accttgccgc cgcggtcctc gatggcctgc ttggcctcgt
    13141 cgcgcgtgaa gccctccagg ccgccggtga gcacgaacgt cttgccggcc aggctgtcgc
    13201 cgaccttggt gacgtcggtg tccatgcgca cgcccagccc ggcgaggtcg tccacgagct
    13261 tggcgttgcg gtcgttgtcg aaccactggc gcaccgccgc ggcgatgatc gggccgacgc
    13321 cggtcacccc ggcgatctcg tcctccgtgg cggcgcgcag cccctccatg gacccgaagt
    13381 ggcgggcgag caggcgcgcg acggtgcccc cgacgtggcg gatgttcagc ccgaccagca
    13441 ggtgctcgaa cggccgggcc ttggacgcct cgatgccggc gaggaggttg tcgaccttgc
    13501 gctcgccgaa ccgctccagc tccagcagct ccgcccgccg gtcggccagc cggtagacgt
    13561 ccgccaggtt ggccaccagc gccttgtcca gcagcacctt ggcggtctcg tagcccagcc
    13621 cctcgatgtc catcgcgccg cggcggtgcg ccagtggttc gaca
//
LOCUS       DATMKC010000379         2372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_130333, whole genome shotgun sequence.
ACCESSION   DATMKC010000379 DATMKC010000000
VERSION     DATMKC010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130333"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VNU01_11135"
     CDS             complement(<1..245)
                     /locus_tag="VNU01_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWH33211.1"
                     /translation="MTLQSDPEWYRTTIIYEVNVRGFSDSNNDGTGDIRGLIERLDYL
                     QWLGIDCIWLLPFYASPLQVVQALDEPADVAGPVVVGV"
     gene            complement(275..2281)
                     /locus_tag="VNU01_11140"
     CDS             complement(275..2281)
                     /locus_tag="VNU01_11140"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HWH33212.1"
                     /translation="MPDRSNAHDTGGHGPRGRVQVQHVSPSVDCGRYAAKAVVGEEIV
                     VSADVFREGHEQVAAAVRYRGPGDRTWRESALRADVNDRWYGSFEADREGPWRYAVCA
                     WTDHYASWLDGLVKKHAAGQDDLALEFEEGARLLERRQGPKAFAQVMAETAELLRSDA
                     ADAEKVAAASADDLVALLERHPEKLDPTTSPELALWVDREKASFSAWYELFPRSEGAE
                     LPQEAGMDGGPARSGTFKEAAKRLPAIADMGFDVVYLPPIHPIGRTHRKGPNNTLTPG
                     PHDPGVPWAIGSEEGGHKAIHPDLGTLEDFRDFVGEAQRLGLEVALDFAIQCSPDHPW
                     VTEHPEWFRHRADGSIAYAENPPKRYQDIFPIDFDTPDQDGLWNELKSVLDHWIAQGI
                     RIFRVDNPHTKALPFWEWVIAEVKREHPDVLLLAEAFTRPKMMQALAKLGFSQSYTYF
                     AWRNSKHELTEYVTELAHSDMADYFRPNFWPNTPDILTEYLVTGGRPAFKIRLLLAAL
                     LSPSYGIYSGYELYENTPLRAGSEEYLDSEKYAYRPRDWDRPDSLAPWIARVNRIRRE
                     HPAFHLLRNVWFHHIGNDALLCFSKVAANRTDAVLVVVNLDPHHVQEGVTWLDMWQLG
                     LEHAGPFEAHDLVTDTTYIWHGPDNYVRLDPHTEPGHVLRLRAL"
BASE COUNT          319 a          825 c          841 g          387 t
ORIGIN      
        1 actccaacaa cgacgggacc ggcgacatcc gcgggctcat cgagcgcctg gactacctgc
       61 agcggcgagg cgtagaacgg cagcagccag atgcagtcga tgcccagcca ctgcaggtag
      121 tccaggcgct cgatgagccc gcggatgtcg ccggtcccgt cgttgttgga gtcggagaag
      181 ccccggacgt tgacttcgta gatgatcgtg gtgcggtacc actccgggtc gctctgcagg
      241 gtcaccgtgt cgtcctcgtc ctcgtcgtcg ccgcctacag cgcgcgcagg cgcagcacgt
      301 gccccggctc ggtgtgcggg tccaggcgca cgtagttgtc cggcccgtgc cagatgtagg
      361 tggtatcggt caccaggtcg tgcgcctcga acggtccggc gtgctccagg cccagctgcc
      421 acatgtccag ccaggtgacg ccctcctgca cgtggtgcgg gtccaggttg accaccacga
      481 gcaccgcgtc ggtgcggttg gccgccacct tgctgaagca cagcagcgca tcgttgccga
      541 tgtggtggaa ccacacgttg cgcagcaggt ggaacgccgg gtgctcccgc cggatgcggt
      601 tcacccgcgc gatccacggt gccagcgagt ccgggcggtc ccagtcgcgc gggcggtagg
      661 cgtacttctc ggagtccagg tactcctcgc tgccggcgcg cagcggcgtg ttctcgtaca
      721 gctcgtagcc ggagtagatg ccgtagctgg gcgacagcag cgccgccagc agcaggcgga
      781 tcttgaacgc ggggcgcccg ccggtgacca ggtactcggt gaggatgtcg ggcgtgttcg
      841 gccagaagtt cggccggaag tagtccgcca tgtccgagtg cgcgagctcg gtgacgtact
      901 cggtgagctc gtgcttggag ttgcgccacg cgaagtaggt gtagctctgg ctgaagccca
      961 gcttggccag cgcctgcatc atcttggggc gcgtgaacgc ctccgccaac agcagcacgt
     1021 cggggtgctc gcgcttgacc tcggcgatca cccactccca gaacggcagc gccttggtgt
     1081 gcgggttgtc cacgcggaag atgcggatgc cctgcgcgat ccagtggtcc agcaccgact
     1141 tgagctcgtt ccacagaccg tcctggtccg gcgtgtcgaa gtcgatcggg aagatgtcct
     1201 ggtagcgctt cggcgggttc tcggcgtagg cgatcgaccc gtcggcgcgg tggcggaacc
     1261 actccgggtg ctcggtcacc caggggtggt cgggggagca ctggatcgcg aagtccagcg
     1321 ccacctccag gccgaggcgc tgcgcctcgc cgacgaagtc gcggaagtcc tccagcgtgc
     1381 ccaggtccgg gtggatcgcc ttgtggccgc cttcctccga gccgatggcc cagggcacgc
     1441 cagggtcgtg cggccccggc gtgagcgtgt tgttggggcc cttgcggtgc gtccgcccga
     1501 tcgggtggat gggcggcagg tacaccacgt cgaagcccat gtcggcgatg gccggcagcc
     1561 gcttcgccgc ctccttgaac gtgccggagc gcgccggccc gccgtccatc ccggcttctt
     1621 ggggcagctc ggcgccctcc gagcgcggga acagctcgta ccaggcggag aagctggcct
     1681 tctcccggtc cacccacagg gccagctcgg gcgaggtggt ggggtccagc ttctccggat
     1741 ggcgctccag cagcgcgacc aggtcgtcgg cggaagccgc cgcgaccttc tccgcgtcgg
     1801 cggcgtcgga gcgcagcagc tcggcggtct ccgccatgac ctgtgcgaac gccttgggcc
     1861 cttggcgccg ctccagcagc cgggcgccct cctcgaactc cagggcgagg tcgtcctggc
     1921 cggcggcgtg cttcttgacc aggccgtcca gccagctggc gtagtggtcg gtccaggcgc
     1981 acaccgcgta gcgccacggg ccttcgcggt cggcctcgaa gctgccgtac cagcggtcgt
     2041 tgacgtcggc gcgcagcgcc gactcgcgcc aggtgcggtc gccggggccg cggtagcgca
     2101 ccgccgcggc cacctgctcg tggccctcgc ggaagacgtc ggccgacacg acgatctcct
     2161 cgccgacgac cgccttcgcg gcgtagcgcc cgcagtcgac cgacgggctg acgtgctgga
     2221 cctggacgcg gccgcgcggg ccgtgtccgc cggtgtcgtg tgcgttggac ctgtctggca
     2281 cggccttcgc tgcctcctcg cggcgaaatc ctgcggggac tccgccctac ccgggcctcc
     2341 gggaccctat cccaaccacc ctgtcaccgg ca
//
LOCUS       DATMKC010000380         2791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_134206, whole genome shotgun sequence.
ACCESSION   DATMKC010000380 DATMKC010000000
VERSION     DATMKC010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134206"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VNU01_11145"
     CDS             complement(<1..1712)
                     /locus_tag="VNU01_11145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWH33213.1"
                     /translation="MSTPTEQGAGELQFLQLDVGGMTCASCAMRIEKKLNKLDGVQAS
                     VNYATEQALVRFPASVAPQDLVAQVEAAGYTATLPTPETAPGASGADGQEEPDPTAAL
                     RTRLLVSLALTVPVLAVSMIPALQFRNWQWLALTLASPVVVWGALPFHRAAWTNARHG
                     TATMDTLISIGTLAAYAWSLVALFLGDAGMPGMRMAFSLTVQRTSGLHEVYLEVAAAV
                     TTFILAGRYYEAKAKRRSGAALKALLGLGAKDVAVLRDGPGGPEEVRIPVDQLRVGMR
                     FVVRPGEKVATDGVVEDGTSAIDASLLTGESVPVEVGPGDAVTGATVNAGGRLVVRAT
                     RVGADTQLAQIARLVTQAQSGKAEVQRLADRVSGVFVPIVIVLALATLVFWLLAGEGA
                     AMAFSAAVAVLIIACPCALGLATPTALLVGTGRGAQMGILIKGPEVLESTRRIDTVVL
                     DKTGTVTSGRMSLVDVTTAPGVSQDDALRLVGALEDASEHPIAQAIAKAARERLGTLP
                     PVESFASAEGLGVQGVVDGVAVVAGRERFLADWALTLPEELRAAKEAAEAAGRTPVLA
                     GWDGA"
     gene            complement(1709..1957)
                     /locus_tag="VNU01_11150"
     CDS             complement(1709..1957)
                     /locus_tag="VNU01_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HWH33214.1"
                     /translation="MSVQTYTVTGMTCGHCVASVSEEVGALDGVTDVQVDLSSGQVTV
                     TSQAPLDDTAVREAVEEAGYSLADGPGGGPRTLPQVSP"
     gene            2084..2356
                     /locus_tag="VNU01_11155"
     CDS             2084..2356
                     /locus_tag="VNU01_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWH33215.1"
                     /translation="MRGYTMDKDDYLKRLKRIEGQVRGLQRMVEEDTYCIDLLTQISA
                     ATKALQSVAIGVLDEHVRHCVAHADGDKAEEMVEEAIRAIERLVRS"
     gene            complement(2408..>2791)
                     /locus_tag="VNU01_11160"
     CDS             complement(2408..>2791)
                     /locus_tag="VNU01_11160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH33216.1"
                     /translation="GQHAAELPPLGEWVAATDERLPEWLRPFGGDALVAWDDDGRYAA
                     GVGIKRHDEHGWEIAVGTEEHARGRGLARRLVATAASAIVARGKVPLYFHAMDNEPSA
                     RVADAAGFPDRGWRLLFVLPRAAEG"
BASE COUNT          388 a         1065 c          977 g          361 t
ORIGIN      
        1 gcgccgtccc agccggccag caccggcgtc cgtccggcgg cctcggccgc ctccttcgcg
       61 gcgcgcagct cctccggcag cgtcagtgcc cagtcagcca ggaagcgctc tcggcccgcg
      121 accaccgcga cgccgtccac gacgccctgc acgcccagac cctccgcgct ggcgaaggac
      181 tccaccggcg gcagcgtccc gagccgctcg cgggcggcct tggcgatcgc ctgcgcgatg
      241 gggtgctcgc tggcgtcctc cagcgccccg accaggcgca gcgcgtcgtc ctggctgacg
      301 ccgggagcgg tggtcacgtc caccaggctc atgcgcccgc tggtgaccgt gccggtcttg
      361 tccagcacga ccgtgtcgat gcgccgcgtg gactccagca cctccgggcc cttgatcagg
      421 atgcccatct gcgcgccgcg gccggtgccc accagcagcg ccgtcggcgt ggccaggccc
      481 agcgcgcacg ggcaggcgat gatcagcacg gccacggcgg ccgagaaggc catggcggcg
      541 ccctcgccgg ccagcagcca gaacaccagc gtggccaggg cgagcacgat cacgatgggc
      601 acgaacacgc ccgagacgcg gtcggccagg cgctgcacct cggccttgcc gctctgcgcc
      661 tgcgtgacca gccgcgcgat ctgcgcgagc tgggtgtccg cgcccacacg ggtggcgcgc
      721 acgaccaggc gcccgcccgc gttgaccgtg gccccggtga cggcgtcacc ggggccgacc
      781 tccacgggca cgctctcacc ggtgagcagc gaggcgtcga tggcgctggt gccgtcctcc
      841 acgacgccgt ccgtggcgac cttctcgccg ggacggacca cgaagcgcat gcccacgcga
      901 agctgatcga ccgggatgcg gacctcttcc gggccgcccg gaccgtcgcg caacaccgcg
      961 acgtccttgg cgccgagccc gagcagcgcc ttgagcgcgg cgcccgagcg gcgcttggcc
     1021 ttcgcctcgt agtagcggcc ggccaggatg aaggtggtga ccgccgcggc gacctccagg
     1081 tagacctcgt gcaggccaga ggtgcgctgc accgtcagcg agaacgccat gcgcatgccc
     1141 ggcatgccgg cgtcgcccag gaacagggcc accagcgacc aggcgtaggc ggccagggtg
     1201 ccgatcgaga tcagggtgtc catggtcgcg gtgccgtgcc tggcgttggt ccacgccgcg
     1261 cggtggaacg gcagcgcgcc ccacaccacc accggcgagg ccagcgtcag cgccagccac
     1321 tgccagttgc ggaactgcag cgccgggatc atcgacacgg ccagcaccgg cacggtcagc
     1381 gccagcgaca ccagcaggcg cgtgcgcagc gcagccgtcg ggtccggctc ctcctgcccg
     1441 tccgcgcccg atgcgccggg tgccgtctcg ggcgtgggca gcgtggcggt gtacccggcg
     1501 gcctcgacct gggccaccag gtcctgcggg gccaccgagg cggggaagcg caccagcgcc
     1561 tgctcggtgg cgtagttcac gctggcctgc accccgtcca gcttgttcag cttcttctcg
     1621 atgcgcatcg cgcacgaggc gcacgtcatg ccgccgacgt ccagttgcag gaactgcagc
     1681 tcgccggcgc cctgctccgt gggtgtgctc atggcgagac ctggggcagc gtgcgcggac
     1741 cgccgccggg gccgtcggcc agcgagtagc cggcctcctc gaccgcctcg cggaccgccg
     1801 tgtcgtcaag cggcgcctgg ctggtgacgg tgacctggcc ggaggacagg tccacctgga
     1861 cgtcggtcac gccgtccagc gctccgacct cctccgacac gctcgcgacg cagtggccgc
     1921 aggtcatccc cgtgacggtg taggtctgga cgctcatgtg cccctccttg gtcgttggcc
     1981 gatgcggtgt gtgcaacaat acccccctgg ggtatattcc gcaacacggc caccggggtg
     2041 gagtacccgt cgccggtatc cttccaccga caaggagggc ggcatgcgcg gctacaccat
     2101 ggacaaggac gactacctca agcgcctgaa gcgcatcgag gggcaggttc gcgggttgca
     2161 gcgcatggtc gaggaggaca cgtactgcat cgacctgctg acgcagatca gtgccgcgac
     2221 caaggcgctg cagtcggtgg ccatcggcgt gctggacgag cacgtgcgcc actgcgtcgc
     2281 gcacgcggac ggcgacaagg ccgaggagat ggtcgaagag gccatccgcg ccatcgagcg
     2341 cctggtccgc tcctagcccc ccggccttcc caacctggaa cgttcgtccg gtactcggac
     2401 gaacggttca accctcggcg gcgcgcggca ggacgaacag caggcgccag ccccggtcgg
     2461 ggaagcccgc ggcgtccgcc acgcgcgccg acggctcgtt gtccatcgcg tggaagtaca
     2521 gcggcacctt gccgcgcgcg acgattgccg acgcggccgt ggccaccagc cggcgcgcca
     2581 gtccgcgccc gcgcgcgtgc tcctcggtgc ccacggcgat ctcccagccg tgctcgtcgt
     2641 ggcgcttgat ccccaccccc gccgcgtagc ggccgtcgtc gtcccaggcg accagggcgt
     2701 cgccgccgaa cgggcgcagc cactccggca ggcgctcgtc cgtggccgcc acccactcgc
     2761 caaggggcgg cagctcggcg gcgtgctggc c
//
LOCUS       DATMKC010000381         3730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_203626, whole genome shotgun sequence.
ACCESSION   DATMKC010000381 DATMKC010000000
VERSION     DATMKC010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203626"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VNU01_11165"
     CDS             <1..349
                     /locus_tag="VNU01_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWH33217.1"
                     /translation="RGFDFATDGTITGRFSNGQTKLLGAVAIATFNNPGGLQKYGENL
                     YSATLNSGQALVGLPGDGDAGVIAPGSLEMSNVDLAQEFTNLIIAQRGFQANARSISS
                     SDEMLADIVNLKR"
     gene            575..826
                     /locus_tag="VNU01_11170"
     CDS             575..826
                     /locus_tag="VNU01_11170"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HWH33218.1"
                     /translation="MILVHRLKGEPMFLNADLIESIEARPDTVVTLVDGRKTVVAETP
                     EEVVDRIRLFRAAVLAAVEDMRAQGPEGGSLVLLRNDEE"
     gene            828..1652
                     /locus_tag="VNU01_11175"
     CDS             828..1652
                     /locus_tag="VNU01_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HWH33219.1"
                     /translation="MNPTFIGGILLSLGAIAGATIIDGNSFGVLFGPSAVVLVFFGTI
                     GASSVGYNVTELKQIPGAITHALKGSPPDHDATINRLVSFANTARREGVLALERRLDE
                     VDDHFQRAGLQLVVDGVDPDQVRQFLEIEIAAADERHQVGISFFKTMAGYAPTMGLLG
                     TIIGLVNMLANLSDPSQLGKGMALALLTTLYGVLFANLVFQPVAERLTRANSAELSSL
                     DMALDGILAVQAGTSPRMLAERLETYLAPERRQGYEARIAAGDPATAGSPVAAPAA"
     gene            1671..2480
                     /locus_tag="VNU01_11180"
     CDS             1671..2480
                     /locus_tag="VNU01_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWH33220.1"
                     /translation="MARGKARGREEHGPKRGGGGGGDGDGGDSRWLTTYGDMVTNLLG
                     FFVILFSMSSVDPARFQSFVEGLYETTFRNESILDGGITIDGELVGPGGEPSGLTPEE
                     QAAEELKVSIEGLRETGAQIEQALQSAGMGGLVDYKIDQRGLVLTIGTDAVLFETGST
                     ELSPQGRDLLAQIAPVLARVPNDIRVEGHTDNVPLNRAGYTNWNLSTDRAVAVLNLLH
                     EVHGVDPVRLLAVGYGEFRPISENETAEGRAANRRVEIIVAVPERRSNVSQ"
     gene            2467..2940
                     /locus_tag="VNU01_11185"
     CDS             2467..2940
                     /locus_tag="VNU01_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HWH33221.1"
                     /translation="MSASSEALAAPAPAAKSGAAKSKLAIIALVLAIAALAFVFLKPG
                     ASAEPAAPPEPQPGPVVDVGQMLVNLADAGRYARVSFALVLAEGTAVGSADGVEANFP
                     LVKEALLFEFSKVTAAQIREPGGLDAIAAALSERARAIYPDGEVMRVVLTELLVQ"
     gene            3061..>3730
                     /locus_tag="VNU01_11190"
     CDS             3061..>3730
                     /locus_tag="VNU01_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33222.1"
                     /translation="MSATSLSDGPLLGDADELDERVPEHYDFRRPSKFGREHVRSLEV
                     AHEVFARRFSSGVAAAMRAMLQLEPLSVDQVSYDDYIRSMPNPSVLTVVTLPPLPGAA
                     IVEMNVQLALVMVDRLLGGKGLPVDVRRPTELETFLLRELMGNALESFRETMEPLLEV
                     APEIASLEFNPQLVQVAAPSDMVLLLSYRASIAQGRRAEGLLTICYPFPTLAPAMNRL
                     VTHLW"
BASE COUNT          554 a         1299 c         1314 g          563 t
ORIGIN      
        1 gcgcggcttc gacttcgcca ccgacggcac catcacgggc cgcttctcca acgggcagac
       61 gaagctcctg ggcgccgtcg cgatcgcgac gttcaacaac ccgggcggcc tgcagaagta
      121 cggcgagaac ctctacagcg cgaccctcaa ctccggccag gcgctggtgg gcctgccggg
      181 cgacggcgac gccggcgtga tcgcccccgg gtcgctggag atgtccaacg tcgacctggc
      241 gcaggagttc accaacctga tcatcgccca gcgcgggttc caggccaacg cgcgcagcat
      301 ttcctccagc gacgagatgc tggccgacat cgtcaacttg aagcgctgac gcttcgcgtc
      361 agcgggccgg cgccgctgga cgttcgctgc ggcgccgggg cacactgcgg ggcggagcag
      421 gcgcgcgtcc aggcgcgttc gcgcgtttcg cccgcctcgt tgccccgccc agcgcctttg
      481 cgggcggggc ggttaagtcc gctcggcaaa agccgagtag atcttgagaa gaatttttcc
      541 gcacggatga gcccacgcac agggaggtgc ccgggtgatc ctcgttcatc gcctcaaggg
      601 cgagccaatg ttcttgaacg cggacctcat cgagtcgatc gaggccaggc cggacaccgt
      661 cgtgacgctg gtggacgggc gcaagaccgt ggtcgcggag acgcccgagg aagtcgtcga
      721 ccgcatccgc ctgttccggg ccgccgtcct ggccgcggtg gaggacatgc gcgcccaggg
      781 ccccgagggc ggctccctgg tgctgttgcg caacgacgag gaatgacatg aaccccacgt
      841 tcatcggtgg gatcctgctg tcgctcggcg cgatcgccgg ggccaccatc atcgacggca
      901 actcgttcgg tgtgctgttc ggcccgtcgg cggtggtgct ggtgttcttc ggcaccatcg
      961 gcgccagctc cgtgggttac aacgtgacgg agctcaagca gatccccggt gccatcacgc
     1021 atgccctcaa gggctcgccg ccggaccacg acgccaccat caaccgcctg gtcagcttcg
     1081 ccaacaccgc gcgccgcgag ggtgtgctgg ccctggagcg gcggctggac gaggtcgacg
     1141 accacttcca gcgcgccggc ctgcaactgg tcgtcgacgg ggtggacccg gatcaggtgc
     1201 gccagttcct ggagatcgag atcgccgcgg ccgacgagcg ccaccaggtg ggcatcagct
     1261 tcttcaagac gatggccggc tatgcgccca cgatgggcct gctcggcacg atcatcggcc
     1321 tggtcaacat gctggccaac ttgagcgacc cctcccagct cggcaagggc atggcgctgg
     1381 cgctgctgac caccctgtac ggcgtgctgt tcgccaacct ggtgttccag cccgtcgcgg
     1441 aaaggctcac gcgcgccaac tccgctgagc tgtcctcgct ggacatggcg ctggacggga
     1501 tcctggccgt ccaggccggc acgagcccgc gcatgctggc ggagcgcctg gagacgtacc
     1561 tggccccgga gcgccgccag ggttacgagg cccggatcgc cgccggggac ccggccaccg
     1621 ccggcagccc cgtcgccgcg cccgcggcct agcggacggg gagaactgcc atggcgcgcg
     1681 gaaaggccag aggccgcgag gagcacggcc ccaagcgcgg cggcggcggg ggcggcgatg
     1741 gcgatggtgg cgactcccgc tggctgacca cctacggcga catggtcacc aacctgctgg
     1801 gcttcttcgt gatcctgttc tcgatgagct cggtcgatcc ggcgcggttc cagagcttcg
     1861 tcgagggcct gtacgagacg acgttccgca acgagtccat cctcgacggc ggcatcacca
     1921 tcgacggcga gctggtcggg cccggcgggg agccgagcgg cctcacgccg gaggagcagg
     1981 ccgccgagga gctcaaggtc tccatcgagg gcctgcgcga gaccggcgcg cagatcgagc
     2041 aggcgctgca gtccgccggc atgggggggc tggtggacta caagatcgac cagcgcgggc
     2101 tggtgctcac gatcggcacg gacgcggtgc tgttcgagac gggatccacc gagttgtccc
     2161 cccaggggcg ggacctgctg gcgcagatcg cgccggtcct ggcccgcgtg cccaacgaca
     2221 tccgcgtcga gggccacacc gacaatgtgc cgctgaaccg ggcgggctac accaactgga
     2281 acctgtccac ggatcgcgcc gtggcggtgc tcaacctgct ccacgaggtg cacggcgtgg
     2341 accccgtccg gctgctggcc gtcgggtatg gggaatttcg tccgatttcc gagaacgaaa
     2401 ctgctgaagg tcgggccgcg aaccgccgag ttgaaatcat cgtcgctgtt ccggagagaa
     2461 ggtccaatgt cagccagtag cgaagcgctt gccgcgcccg cgcccgcggc caagtcgggg
     2521 gcggccaaga gcaagcttgc gatcatcgcc ctcgtgctcg cgatcgccgc cttggcgttc
     2581 gtgttcctca agcccggcgc ctcggccgag cccgccgcgc cgcccgagcc gcagcccggg
     2641 ccggtggtgg acgtgggcca gatgctggtg aacctggccg acgcgggccg ctacgcgcgc
     2701 gtgtcgttcg ccctggtgct cgccgagggg accgccgtgg gctccgccga cggcgtggag
     2761 gcgaacttcc cgctggtcaa ggaagcgctg ctgttcgagt tctccaaggt gaccgccgcg
     2821 cagatccgcg agcccggcgg gctcgacgcg atcgcggcgg cgctgagcga gcgcgcgcgc
     2881 gcgatctatc cggacggcga ggtcatgcgc gtggtcctca cggagctgct cgttcagtaa
     2941 ccgcgcgatt cccgctttac tccggtgccc gggggtcgac aggacccgcg ggcaccgcct
     3001 gttccggcgt gcgccatgcg agcgttgtgc cagccaggcc acccgcgcga gaggagcgtg
     3061 atgtccgcga ccagcttgtc cgacgggccc ctgctcggcg atgccgacga gctggacgag
     3121 cgtgttccgg agcactacga cttccgccgc cccagcaagt tcggccgcga gcacgtgcgc
     3181 agcctcgaag tcgcgcacga ggtgttcgcg cgccggttct ccagcggcgt cgcggcggcc
     3241 atgcgggcga tgctgcagct ggagccgctg tcggtcgacc aggtcagcta cgacgactac
     3301 atccgttcga tgccgaaccc gtccgtgctg acggtggtga cgctgccgcc gctgccgggc
     3361 gccgcgatcg tcgagatgaa cgtccagctc gccctggtga tggtcgaccg cctgctgggc
     3421 ggcaagggcc tgcccgtgga cgtgcgccgc cccacggagc tggagacgtt cctgctgcgc
     3481 gagctcatgg gcaacgcgct ggagtcgttc cgcgagacca tggagccgct gctggaggtc
     3541 gcgccggaga tcgccagctt ggagttcaac ccgcagctcg tgcaggtcgc cgcgccgtcg
     3601 gacatggtgc tgctgctgtc ctaccgcgcc agcatcgccc agggccgccg cgccgagggc
     3661 ctgctgacca tctgctatcc gttcccgacg ctggctccgg cgatgaaccg cctggtcacg
     3721 cacctgtggg
//
LOCUS       DATMKC010000382         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_274167, whole genome shotgun sequence.
ACCESSION   DATMKC010000382 DATMKC010000000
VERSION     DATMKC010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274167"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VNU01_11195"
     CDS             <1..216
                     /locus_tag="VNU01_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33223.1"
                     /translation="VPVDRTVAALAGRAAVDLAQDAAAESGLMVALRDGAGAWRAEGI
                     PLERVRGERTLPPALQADPRPLAAWLS"
     gene            232..1596
                     /locus_tag="VNU01_11200"
     CDS             232..1596
                     /locus_tag="VNU01_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HWH33224.1"
                     /translation="MSRRVDEVSLSRRAFLGAAGALVIPGRRGAQEGQGWRAGAAALD
                     ITPEGSLWMAGFAARKEPARGVALPLRAKALALQAADGAPAVLVTVDLLGVTARLTGA
                     VAAEAARRHGVARSHLLFNASHTHCGPIVDAQLAVAYELTPGQWEDIRVYTAQLEGKL
                     VAVIGEALGRLAPARLAYARGEAAFARNRRVAFTPDGPVDHSVPVLRVAGADGATLAV
                     VFGYACHNTTLQAGFVQYHGDYAGVAQAAVERRHPGVTALFVAGCGADANPHPRGTIE
                     LVEAHGAALADAVLRALPAAAPLPPGLRAAYGTVDLPFAGAEVRERWMAGLDIDPVYL
                     RRHAALMKEIAAREGSLPAAQAAPIQVWRLGPGTGSELMLVAIGGEVVVDYALRIGRE
                     YPDRRTWAAGYSNDVFGYLPSRRVLREGGYEGADAMIYYGRPAPFTDAVEDLIMGEVR
                     RLAR"
     gene            1821..>2358
                     /locus_tag="VNU01_11205"
     CDS             1821..>2358
                     /locus_tag="VNU01_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWH33225.1"
                     /translation="MADSTEERALVALVRGGKWFRGEQVAAFEAAYAALTGARGCLAT
                     ANGTSALITSLAALGIGAGDEVIVPPYTFIATVNAVLLMGALPVFVDTDRETFQIDAR
                     RIEEAITPATRAIVPVHLGGSAADLDTVMDVARRRGLTVVEDACQAHLAEWRGRKVGT
                     YGAAGCFSFQASKNLNSGE"
BASE COUNT          295 a          809 c          942 g          312 t
ORIGIN      
        1 gtgccggtgg atcggacagt ggccgcactc gccgggcgcg cagcggtgga tctcgcgcag
       61 gacgcggcgg cggagagcgg gctcatggtc gcgctccgcg acggcgccgg cgcgtggagg
      121 gccgagggga tcccgctcga acgcgtccgc ggcgagcgta cgctgccccc ggcgctccag
      181 gccgatccgc gcccgctggc ggcgtggtta tcgtgaatgg agaggaaccg ggtgagcaga
      241 cgcgttgacg aggtgagcct gagccggcgt gcgttccttg gtgcggccgg cgccctggtg
      301 attcccgggc gccgtggcgc gcaggaggga caggggtgga gggcgggagc cgccgccctc
      361 gacatcaccc cggagggatc gctctggatg gcgggcttcg ccgcgcggaa ggagcccgcc
      421 cgcggcgtgg cgctccccct gcgggcgaag gcgctcgccc ttcaggcggc ggacggcgcg
      481 cctgcggtgc tcgtgacggt ggacctgctg ggcgtgaccg cccgcctgac cggcgccgtg
      541 gcggcggagg ctgcgcggcg gcacggcgtg gcgcgatcgc acctcctctt caacgcgagc
      601 cacacgcact gcgggccgat cgtggacgcg cagcttgccg tggcgtacga gctgaccccg
      661 gggcagtggg aggacatccg tgtgtacacc gcgcagctcg aagggaagct ggtggcggtc
      721 atcggcgagg cgctcggccg cctggctccc gcccggctcg cgtacgcgcg gggcgaggcg
      781 gcgttcgcga ggaaccggcg cgtggcgttc acgcccgacg gaccggtgga ccactcggtg
      841 cccgtcctgc gcgtggccgg tgcggacggc gcgacgctgg ccgtcgtctt cggatatgcg
      901 tgtcacaaca ccacgctgca ggcgggcttc gtgcagtatc acggcgatta tgccggcgtc
      961 gcccaggcgg ccgtcgagcg gcggcacccg ggagtgacgg cgctgttcgt ggccggctgc
     1021 ggcgccgacg cgaacccgca tccgcgaggc accatcgagc tggtggaggc gcacggggcg
     1081 gcgcttgccg acgcggtcct ccgcgcgctg ccggcggcgg cgccgctccc gccggggctg
     1141 cgcgccgcgt acgggacggt ggacctgccg tttgccggcg ccgaggtgcg cgaacgctgg
     1201 atggcggggc tcgacatcga cccggtgtat ctccgccgtc acgcggcgct gatgaaggag
     1261 atcgccgcgc gcgagggaag cctcccggcg gcgcaggcgg cccctatcca ggtgtggcgg
     1321 ctcgggcccg gcaccggatc cgagctgatg ctggtcgcga tcggcgggga ggtggtggtg
     1381 gactacgcgc tccgcatcgg gcgcgagtat ccggaccgcc gcacatgggc tgcgggctac
     1441 agcaatgacg tgttcgggta cctgccgtcc cgccgcgtgc tgcgcgaggg aggatacgaa
     1501 ggggccgacg cgatgatcta ctacggccgg ccggcgccgt tcaccgacgc ggtcgaggac
     1561 ctgatcatgg gtgaggttcg ccggctcgcc cggtgatccg gggaactgga atcgagggaa
     1621 acgtcatgcg cgagagaaaa ccggtcagcc gccgtgcctt catcgcagcc gcggtcgcgg
     1681 ggagcgccgc ggccggcacc agccctgccg gcgggcaggg gccgtccggg gcggccgcgc
     1741 ccgtggtgcg ccgcaccgag gcaggcccgg cgctcctcgg cggcacgccg gtgcggcggg
     1801 cgccattccc tgcctggccc gtggccgaca gcaccgaaga gcgtgcgctc gtggcgctgg
     1861 tgcgcggcgg caagtggttc cgcggcgagc aggtcgccgc gttcgaagcg gcgtatgccg
     1921 cgctgacggg tgccaggggg tgcctcgcga ccgccaatgg caccagcgcc ctcatcacct
     1981 cgctggccgc gctcgggatc ggcgccggcg acgaggtgat cgtccccccg tataccttca
     2041 tcgccaccgt gaacgcggtg ctcctcatgg gggccctgcc ggtgttcgtc gacaccgacc
     2101 gcgagacgtt ccagatcgac gcgcgccgca tcgaggaggc gatcacgccg gccacgcgcg
     2161 ccatcgttcc cgtgcacctg gggggatccg ccgccgacct cgacacggtg atggacgtgg
     2221 cccgccggcg gggcctgacc gtcgtggagg acgcctgcca ggcgcatctc gcggaatggc
     2281 gcggccggaa ggtgggcacg tacggggcgg ccggctgttt cagctttcag gcgagcaaga
     2341 acctgaactc cggggaag
//
LOCUS       DATMKC010000383         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_199990, whole genome shotgun sequence.
ACCESSION   DATMKC010000383 DATMKC010000000
VERSION     DATMKC010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199990"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VNU01_11210"
     CDS             complement(<1..183)
                     /locus_tag="VNU01_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33226.1"
                     /translation="MSLRTRVALIAATAVAVSVLAVSVGAYLATARTLHGGVDASLLD
                     QAEQLLGLGGRPRMGPR"
     gene            complement(180..860)
                     /locus_tag="VNU01_11215"
     CDS             complement(180..860)
                     /locus_tag="VNU01_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH33227.1"
                     /translation="MRILIVDDDRAVRDSLRRALELHGYEAETAPDGIEALRSVQRQP
                     PDAMILDVNMPGVDGFGLTRRLRQDGVHLPILMLTARDAVPDRVAGLDTGADDYLVKP
                     FALEELLARLRVLLRRAGAEDRPAQAGVLRFADLELDPQRVSVRRGERELALSRTEYE
                     LLLAFMENPGRVLTRDVLHERVWGADMSASNTLEVYVGYLRRKLEAAGEPRLVQTVRG
                     FGYALREP"
     gene            complement(965..1438)
                     /locus_tag="VNU01_11220"
     CDS             complement(965..1438)
                     /locus_tag="VNU01_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33228.1"
                     /translation="MAITRTRLAAIGVAGVLSAGAAVGALSPAIAQDAEEAPSTDTRA
                     QERAERHEQRREAFASALAEELDLPVERVGTALDNVREQMRAEAEEQRLAHLRERLDA
                     AVADGRLTQEQADAIYAAAESGAMPFGGRPGGHHRGVRGGPPPAGGGTEADATSA"
     gene            complement(1551..>2343)
                     /locus_tag="VNU01_11225"
     CDS             complement(1551..>2343)
                     /locus_tag="VNU01_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HWH33229.1"
                     /translation="RGRVPVGVPHRQGGAADRRSHLRANALVGNRPEAAALELVAPGF
                     ACRFHADAVCALTGAPAAATLDGDPLPHDTVFTARAGQRLDLGPAPWGLRTVLAVRGG
                     IAVEPVLGSRATDTLAGLGPAPLRARDRLPVGGLTGEQDWRAPAPAVAPAQPLEVPVV
                     AAPGAAWVDTAALAESLATVTAASGRTALRLDAPALARRRTEEFPPQALVRGAVQVPP
                     DGRPIVFLADHPVTGGYPVIGVVPAAHNDRLAQLRPGDRLRLVLR"
BASE COUNT          259 a          873 c          941 g          270 t
ORIGIN      
        1 acggggcccc atgcgcggcc tgccgcccag gccgagcagc tgctcggcct ggtcgagcag
       61 cgacgcgtcg acaccgccgt gcagggtgcg cgccgtcgcc aggtaggcgc ccacggacac
      121 cgccaggacg gacaccgcga cggcggtcgc cgcgatgagc gcgacgcgcg tgcgcaggct
      181 cacggctcgc gcagcgcgta gccgaagccg cgcacggtct ggacgaggcg cggctccccg
      241 gcggcctcga gcttgcgacg caggtagccg acgtagacct ccagggtgtt ggacgcgctc
      301 atgtcggcgc cccacacgcg ctcgtgcagg acgtcgcggg tgagcacgcg tcccgggttc
      361 tccatgaacg ccagcagcag ctcgtactcg gtgcggctga gcgccagctc gcgctcgccg
      421 cgtcgcacgc tcacgcgctg cgggtccagc tccagatcgg cgaagcgcag cacgcctgcc
      481 tgcgcgggac ggtcctccgc gccggcgcgg cgcagcagca cccgcagccg tgccaggagc
      541 tcctccagcg cgaacggctt cacgaggtag tcgtcggcgc cggtgtccag gcccgccacg
      601 cggtccggca ccgcgtcgcg cgccgtcagc atgaggatcg gcaggtggac gccgtcctgg
      661 cgcaggcggc gggtgagccc gaagccgtcc acgcccggca tgttgacgtc gaggatcatc
      721 gcgtcggggg gctggcgctg gaccgagcgc agcgcctcga tgccgtcggg cgcggtctcg
      781 gcctcgtagc cgtgcagttc cagcgcgcgg cgcaggctgt cgcgcacggc gcggtcgtcg
      841 tcgacgatca ggatccgcat gcgccacagc ctgccaggcg cggctgagaa cggggtgagg
      901 ggggcccggg acgcgagggg ggcggtcgcg tcccgggccc ccccggggtg ggagctgggt
      961 ggtgtcaggc cgaggtggcg tccgcctcgg tgccgccgcc ggccggcggc gggccgccac
     1021 gcacgccgcg atggtggccg cctgggcgcc cgccgaaggg catcgcgccg ctctccgcgg
     1081 cggcgtagat cgcgtccgcc tgctcctggg tcagccgccc gtccgccacg gcggcgtcga
     1141 ggcgctcgcg caggtgcgcc agccgctgct cctcggcctc cgccctcatc tgctcgcgga
     1201 cgttgtcgag cgcggtgccg acgcgctcca cgggaaggtc cagctcctcc gccagcgccg
     1261 aggcgaacgc ctcgcggcgc tgctcgtggc gctcggcgcg ctcctgcgcg cgggtgtcgg
     1321 tgctcggcgc ctcctcggcg tcctgcgcga tggcggggct cagggcgccc acggcggcgc
     1381 ccgcgctgag cacgcccgcc acgccgatcg cggcgagccg tgtccgggtg atcgccatgc
     1441 tgtctccttt gggttcggtc cgctgttgac gatcacaccg tcggcaatgc gcgtgagagg
     1501 gcgctgagag cggcgtgaga gtttccggcc agggcccggc gcggtcgctg tcagcgcagg
     1561 acgaggcgca ggcggtcgcc cggacgcagt tgcgccaggc ggtcgttgtg cgcggcgggg
     1621 acgacgccga tgacgggata gccgccggtc acggggtggt cggccaggaa cacgatgggc
     1681 cgtccgtccg gcggcacctg cacggcgccg cgcacgagcg cctgcggggg gaactcctcg
     1741 gtccggcggc gcgccagcgc cggggcgtcc aggcgcaggg cggtccgccc gctcgccgcg
     1801 gtcacggtgg caaggctctc ggccagcgcg gcggtgtcga cccaggcggc gcccggcgcg
     1861 gccacgaccg ggacctccag cggctgggcc ggggcgacgg cgggcgcggg cgcgcgccag
     1921 tcctgctcgc cggtgaggcc gcccaccggg aggcggtcgc gggctcgcag cggcgcgggg
     1981 cccagcccgg cgagcgtgtc cgtggcgcgg ctgcccagca ccggctcgac cgcgatgccg
     2041 ccgcgcacgg ccagcaccgt gcgcaggccc cacggcgcag ggccgaggtc gaggcgctgg
     2101 ccggcgcgcg cggtgaacac ggtgtcgtgc ggcagcgggt cgccgtccag cgtggcggcc
     2161 gcgggcgcgc cggtgagcgc gcacaccgcg tcggcgtgga agcggcacgc gaagcccggg
     2221 gccacgagtt cgagcgccgc ggcttcgggg cggttgccca ccagcgcgtt ggcgcgcaga
     2281 tgggacctgc ggtcggccgc accgccctga cgatgtggga cgccgaccgg gacccgcccg
     2341 cgc
//
LOCUS       DATMKC010000384         3595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_345340, whole genome shotgun sequence.
ACCESSION   DATMKC010000384 DATMKC010000000
VERSION     DATMKC010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345340"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VNU01_11230"
     CDS             <1..238
                     /locus_tag="VNU01_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HWH33230.1"
                     /translation="IQPVSLVPSRRRGPRRREGTKDTGWMILDFGDVVFHGFVAEQRE
                     FYNLERLWADAPQVEFTDAGAPPEALAHVEASGE"
     gene            235..924
                     /locus_tag="VNU01_11235"
     CDS             235..924
                     /locus_tag="VNU01_11235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWH33231.1"
                     /translation="MSEGAYPPESVGETPPGSATLVLVRHGESRWNAAGVLQGQLCEG
                     LSEHGRLQAAAVAEYLVKAHGDATLIARSDIDRVAQTAAPAEERLGLQVLIEPRLREI
                     DLGSWSGRPRAEVLAEHHAELMGWLRCEDVRAGGSGETLTELRERVWAAMEDLGDRLA
                     GEGGGSAILFTHGGPVRTTTAAVLGLPPGAERRIQPVRNGSVTVVRRDPDGAGGHEWR
                     LVAYNRADHLP"
     gene            985..2106
                     /locus_tag="VNU01_11240"
     CDS             985..2106
                     /locus_tag="VNU01_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH33232.1"
                     /translation="MRVLLVDDHATLRVALRALLDEVAGIEVVGECADGSEVVEAVAA
                     LRPELVLMDINMPVVDGIEATLAVKRAYPDVRVVGLSGFEDATKVSAMIAAGAGGYLL
                     KTADPREIAEGLQSILAGRPVLAPEVLQGVLDDVARFYREQLAKAELLAEVDRMKRQF
                     FTLVSDDLRNPLAAITGYARTMRQNWTRIDEATREEFTERIEQQSLRLGKRIEQMLTI
                     SGIRRAEPWEATEFDLGAAAREAAERFDDRRLSVRALDSMLVRGDRGGFVTAAVTLIE
                     NALQHTQGVVLLGTGREAKMATLMVRDEGPGMEPERLRWLLAGPFARIEQGEELEEGG
                     SLGLSIYIARMVLEAMGGVLEAESSPIGGSAFVALVPLA"
     gene            complement(2176..>3595)
                     /locus_tag="VNU01_11245"
     CDS             complement(2176..>3595)
                     /locus_tag="VNU01_11245"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HWH33233.1"
                     /translation="ERGISISSTVLHFAFGPWRFNLLDTPGHADFSEDTYRTLWAADA
                     AIMVIDVAKGLEPQTLKLFRVCRDRGIPIVTFVNKCDRPGLPPLAILDEISAEIGMEP
                     VPMVWPVADGPEFAGVVDRLSEEFVAFGRSAHGSKQAEETRTPLDKVQRAEVAPQTPQ
                     LRWDTALEELELLDIEERALDRAAFLAGKQTPVFFGSALANFGVRQLLEGFANLAPSP
                     GPQAVAEPEGAPARDVTAPFSALVFKIQANMDPRHRDRVAFLRVQSGRFARGMTAVHA
                     RTGRPYALKYAHQLFAQDRETVDDAVAGDVVGVVNATDLLVGDTLYAEDKVVFPPIPT
                     FAPELFVGARNRDTSRYKQFRRGLAQLDEEGVVHVLQRPEYGEQEPLLAGVGQLQFEV
                     AEHRMREEFGAQIALTPAPWQLARLLVDGDAAKLRGQRGTLVVVDSKGRTLALFNSEH
                     ALTWAMQDHQDLVFGELGMTRS"
BASE COUNT          484 a         1270 c         1359 g          482 t
ORIGIN      
        1 catccagccg gtgtccttgg tgccctcccg gcgccgcggc ccgcggcgcc gggagggcac
       61 caaggacacc ggctggatga tcctggactt cggcgacgtg gtgttccacg gcttcgtggc
      121 cgagcagcgc gagttctaca acctcgagcg gctgtgggcc gacgcgccgc aggtggagtt
      181 caccgacgcc ggcgcgccgc cggaggcgct ggcgcacgtg gaggcgtccg gcgagtgagc
      241 gagggcgcgt acccgccgga gtccgtcggt gagacgccgc cgggctcggc gacgctggtg
      301 ctggtgcgcc acggcgagtc ccgctggaac gccgcggggg tgctgcaggg ccagttgtgc
      361 gagggactgt ccgaacacgg ccgcctgcag gccgccgccg tggcggagta cctcgtcaag
      421 gcgcacggcg acgcgacgct gatcgcccgc agcgacatcg accgcgtcgc ccagaccgcc
      481 gcgcccgccg aggagcgcct cggcctgcaa gtcctgatcg agccgcgact gcgcgagatc
      541 gacctggggt cctggagcgg gcggccgcgc gccgaggtgc tggccgagca ccatgccgag
      601 ctcatgggct ggctgcgctg cgaggacgtg cgcgcgggcg gcagcggcga gacgctgacc
      661 gagttgcgcg agcgggtctg ggcggcgatg gaggacctcg gggaccggct ggccggggaa
      721 ggcggcggca gcgcgatcct gttcacgcac ggtgggcccg tccgcaccac cacggcggcc
      781 gtcctgggcc tgccgccggg cgccgagcgc cgcatccagc cggtgcgcaa cggctcggtg
      841 acggtggtgc gccgcgatcc cgacggtgcc ggcggccacg agtggcgcct ggtggcctac
      901 aaccgcgccg accacctgcc ctaggagcac ctgtctggac gcggatcgcc gccgcgccgg
      961 gcggtccccc gtaggcttga gccgatgcgt gtcctgctgg tcgacgacca cgccaccctg
     1021 agggtggcgc tgcgcgcgct gctcgacgag gtggccggca tcgaggtggt gggggagtgc
     1081 gccgacggtt ccgaggtggt cgaggccgtc gcggcgctgc ggcccgagct ggtcctcatg
     1141 gacatcaaca tgccggtggt ggacggcatc gaggcgacgc tggccgtcaa gcgcgcctac
     1201 ccggacgtgc gcgtggtggg gctgtcgggc ttcgaggacg ccaccaaggt gtcggccatg
     1261 atcgccgcgg gcgccggcgg ctacctgctc aagaccgcgg acccgcgcga gatcgccgaa
     1321 gggctgcagt ccatcctggc gggccggccg gtgctcgcgc cggaggtgct ccagggcgtg
     1381 ctggacgacg tcgcgcgctt ctaccgggag cagctcgcca aggcggagct cttggccgag
     1441 gtggaccgca tgaagcgcca gttcttcacg ctggtgtccg acgacctgcg caacccgctg
     1501 gcggcgatca ccggctacgc gcgcacgatg cgccagaact ggacgcgcat cgacgaggcc
     1561 acccgcgagg agttcaccga gcgcatcgag cagcagtccc tgcgcctggg caagcggatc
     1621 gagcagatgc tcacgatctc cggcatccgc cgcgccgagc cgtgggaggc cacggagttc
     1681 gacctcggcg ccgcggcacg ggaggcggcg gagcgcttcg acgaccggcg cctgtccgtg
     1741 cgcgcactgg actccatgct cgtgcgcggc gatcgcgggg gcttcgtgac cgctgccgtg
     1801 acgctgatcg agaacgcgct ccagcacacc cagggcgtgg tgctgctggg gacggggcgc
     1861 gaggccaaga tggccacgct catggtgcgc gacgagggcc ccggcatgga gcccgagcgg
     1921 ctgcgctggc tgctggccgg cccgttcgcg cgcatcgagc agggcgagga gctggaggag
     1981 ggcggcagcc tggggctgtc gatctacatc gcccgcatgg tgctggaggc gatgggcggc
     2041 gtgctggagg cggagtcctc gccgatcggt ggctcggcct tcgtcgcgct ggtccccctg
     2101 gcctgaccca cccgcaagac ccgctgcggg acgagttgtg tccgataacg gccacaagtg
     2161 gtcccgcagc gtgggctagg agcgggtcat gcccagttcg ccgaagacca ggtcctggtg
     2221 gtcctgcatc gcccaggtca gcgcgtgctc ggagttgaac agcgccagcg tgcgcccctt
     2281 ggagtccacc accaccagcg tgccgcgctg cccgcgcagc ttggcggcgt cgccgtccac
     2341 gagcagccgc gcgagctgcc acggggccgg cgtgagcgcg atctgcgcgc cgaactcctc
     2401 ccgcatgcgg tgctcggcga cctcgaactg cagctggccc acgccggcca gcagcggctc
     2461 ctgctcgccg tactcggggc gctggagcac gtgcacgacg ccctcctcgt ccagctgcgc
     2521 caggccgcgg cggaactgct tgtagcggct ggtgtcgcgg ttgcgcgcgc cgacgaacag
     2581 ctcgggggcg aacgtcggga tcggcgggaa cacgaccttg tcctcggcgt acagcgtgtc
     2641 gccgaccagc aggtccgtgg cgttcaccac gccgaccacg tcgccggcca ccgcgtcgtc
     2701 gaccgtctcg cggtcctgcg cgaagagctg atgggcgtac ttgagcgcgt agggccggcc
     2761 cgtgcgcgcg tgcacggccg tcatgccgcg ggcgaagcgc ccggactgca cgcgcaggaa
     2821 cgcgacgcgg tcacggtggc gggggtccat gttcgcctgg atcttgaaca cgagcgcgct
     2881 gaacggcgcg gtcacgtcgc gcgcgggcgc gccctccggc tcggcgacgg cctgcgggcc
     2941 gggggaaggc gccaggttcg cgaagccctc caggagctgg cgcaccccga agttcgccag
     3001 cgcggagccg aagaacaccg gcgtctgctt gccggccagg aacgccgccc ggtccagcgc
     3061 gcgctcctcg atgtccagca gctccagctc ctccagcgcg gtgtcccagc gcagctgcgg
     3121 cgtctgcggc gcgacctcgg cgcgctgcac cttgtccagg ggcgtgcgcg tctcctcggc
     3181 ctgcttggag ccgtgcgcgc tgcggccgaa cgcgacgaac tcctccgaca ggcggtccac
     3241 cacgccggcg aactccggcc cgtcggccac cggccacacc atcggcaccg gctccatgcc
     3301 gatctcggcg gagatctcgt ccaggatcgc cagcggcggc aggcccggcc ggtcgcactt
     3361 gttgacgaac gtcacgatcg ggatgccgcg gtcacggcag accctgaaca gcttcagggt
     3421 ctgcggctcc aggcccttgg cgacgtcgat caccatgatc gcggcgtcgg ccgcccacag
     3481 cgtgcggtag gtgtcctcgg agaagtcggc gtggccgggc gtgtccagca agttgaagcg
     3541 ccacggcccg aacgcgaagt gcagcaccgt ggagctgatc gagatgccgc gctcc
//
LOCUS       DATMKC010000385         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_62646, whole genome shotgun sequence.
ACCESSION   DATMKC010000385 DATMKC010000000
VERSION     DATMKC010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62646"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VNU01_11250"
     CDS             <1..551
                     /locus_tag="VNU01_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWH33234.1"
                     /translation="RYAAGVRLPFRLLVLVAVALAQAACSGEQGGEVAIAPDDPGRPA
                     LQPSEPFTRVVSLDWLGPGGARASLDAWVADTPEKRALGLMHRTALPEDAGMVFVFAQ
                     DHAGGFWMRNTRIPLSIAHLSADGEVLVVLDMEPCQTDPCPVYDPGIAYRYAVEANQG
                     WFAEHGIGPGWRVLGGLPPASG"
     gene            600..740
                     /locus_tag="VNU01_11255"
     CDS             600..740
                     /locus_tag="VNU01_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33235.1"
                     /translation="MDTWLMIVISAAVVLLPFVLMFAMNGRNEADSRGRRLDRTWRTS
                     HK"
     gene            865..>2356
                     /gene="dnaK"
                     /locus_tag="VNU01_11260"
     CDS             865..>2356
                     /gene="dnaK"
                     /locus_tag="VNU01_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWH33236.1"
                     /translation="MAKAVGIDLGTTNSVVAVLEGGEPTVIANAEGARTTPSVVGFSK
                     AGEVLVGEVAKRQAVTNPDRTIQSVKRHMGTDWATEVDGKSYTAQEISARILMKLKRD
                     AEAYLGEEVTQAVITVPAYFDDAQRQATKEAGQIAGLEVLRIINEPTAAALAYGLDKE
                     GEQTILVYDLGGGTFDVSVLEIAEGVFDVKSTSGDTHLGGDDWDQAVIDWLVKSFKDK
                     HGVDLAADKMALQRLKEAAEKAKKELSSTMETSINLPFITATSEGPLHLEESLTRAQF
                     QQMTADLLERTKQPYFNAIKDAGIKPDDIDHVILVGGSTRMPAVADLVKELTGGKEAH
                     KGVNPDEVVAIGASLQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTKLIERNTTIPT
                     RKSEVFTTAEDNQSQVEIHVLQGEREMASFNKTLGKFQLVDIPPAPRGVPQIEVTFDI
                     DANGIVHVSAKDRGTGKEQSMTITGQSALPKDDIERMVRDAEAHAEE"
BASE COUNT          406 a          814 c          813 g          323 t
ORIGIN      
        1 ctcgctacgc tgcgggggtg cgccttcctt tccgcctgct cgtcctcgtt gctgtcgcgc
       61 tcgcgcaggc cgcgtgctcc ggcgagcagg gcggtgaggt ggccatcgct cccgacgatc
      121 cggggcggcc ggcgctgcag ccctcggagc cgttcacgcg ggtcgtgtcg ctggactggt
      181 tggggcctgg cggcgcccgc gcgagtctgg acgcctgggt ggcggacacg cctgagaagc
      241 gcgctctggg gttgatgcac cgcacggcac tgccggagga cgcgggcatg gtgttcgtgt
      301 tcgcccagga ccacgccggc ggcttctgga tgcgcaacac gcgcatcccg ctgtcgatcg
      361 cgcacctgtc cgccgacggc gaagtcctgg tcgtgctgga catggagccg tgccagaccg
      421 atccctgccc ggtctacgac ccggggatcg cctaccgcta cgccgtggag gcgaaccagg
      481 ggtggttcgc cgagcacggc atcggccccg gatggcgtgt gctgggcggg ctgcctcccg
      541 cttccggatg accgcgcggc cggttcccct ccctgccgcc gggcggtagc ctgcccgcca
      601 tggacacctg gctgatgatc gtcatctccg cggcggtcgt gctgctgccg ttcgtgctga
      661 tgttcgccat gaacggcagg aacgaggcgg acagccgcgg ccgccgcctc gaccgcacct
      721 ggcgcacttc ccacaaatag aaaaactgag cgtctcacgc tcaagtatgt tgccaccctc
      781 gcgcgcgacg ggtagccttg tccacgtcac gcaaggtttt cacgacgcat cgcccgccgt
      841 gcgcgggccg gaggtggcaa cgacatggcc aaggcagtcg gcatcgacct gggcaccacc
      901 aactccgtgg tggccgtgct cgagggcggc gagcccaccg tcatcgcgaa cgccgagggc
      961 gcgcgcacca cgccgtccgt ggtcggcttc tccaaggcgg gcgaggtact ggtgggcgag
     1021 gtcgccaagc gccaggccgt cacgaacccg gaccggacca tccagtcggt caagcggcac
     1081 atgggcaccg actgggccac cgaggtggac ggcaagagct acaccgcgca ggagatcagc
     1141 gcccgcatcc tgatgaagct gaagcgggac gcggaggcct acctgggcga ggaggtcacg
     1201 caggcggtca tcaccgtgcc ggcctacttc gacgacgcgc agcgccaggc caccaaggag
     1261 gccgggcaga tcgccggcct ggaggtcctg cgcatcatca acgagccgac cgcggcggca
     1321 ctggcctacg gcctggacaa ggaaggcgag cagaccatcc tggtctacga cctgggcggc
     1381 ggcaccttcg acgtgtccgt gctggagatc gccgagggcg tgttcgacgt caagtccacc
     1441 agcggcgaca cccacctggg cggcgacgac tgggaccagg ccgtcatcga ctggctggtg
     1501 aagtccttca aggacaagca cggggtcgac ctcgccgcgg acaagatggc gctgcagcgc
     1561 ctgaaggaag ccgccgagaa ggccaagaag gagctctcct ccacgatgga gacctccatc
     1621 aacctgccgt tcatcacggc gaccagcgaa ggcccgctgc acctggagga gtcgctcacg
     1681 cgcgcccagt tccagcagat gaccgcggac ctgctggagc gcaccaagca gccctacttc
     1741 aacgccatca aggacgccgg catcaagccg gatgacatcg accacgtgat cctggtcggc
     1801 ggctccacgc gcatgcccgc cgtcgcggac ctggtgaagg agctcacggg cggcaaggag
     1861 gcgcacaagg gcgtcaaccc cgacgaggtc gtggccatcg gcgcgtcgct gcaggccggc
     1921 gtgctcaagg gcgaggtcaa ggacgtcctg ctgctggacg tcacgccgct gtcgctgggc
     1981 atcgagacca agggcggcgt gatgaccaag ctgatcgagc gcaacaccac catcccgacg
     2041 cgcaagtccg aggtcttcac caccgccgag gacaaccaga gccaggtgga gatccacgtg
     2101 ctgcagggcg agcgcgagat ggccagcttc aacaagacgc tgggcaagtt ccagctcgtc
     2161 gacatcccgc ccgccccgcg cggcgtgccg cagatcgagg tcacgttcga catcgacgcc
     2221 aacggcatcg tgcacgtgtc cgccaaggac cgcggcaccg gcaaggagca gtcgatgacg
     2281 atcaccggcc agtccgcgct gcccaaggac gacatcgagc gcatggtgcg cgacgccgag
     2341 gcgcacgcgg aggagg
//
LOCUS       DATMKC010000386         8109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_24179, whole genome shotgun sequence.
ACCESSION   DATMKC010000386 DATMKC010000000
VERSION     DATMKC010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24179"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VNU01_11265"
     CDS             complement(<1..1112)
                     /locus_tag="VNU01_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWH33237.1"
                     /translation="MTMAPDRPPSVASRLPDIPGPGETALRLWRRLRKMSTALVLLFL
                     LAAASVLATFIPQRPLIPSTVADWLAGEAGPGAGVAQVLDWLGLFDVFGATWFAALVL
                     LLFTSLTGCLLPRWRGFLRVVRKAPPAGRNLGRLTHSEQWRTALPPERALEAAAGALR
                     GYRRRRCADATASGAAQVAAERGHWREGGSLVFHTAFYLLLAGAVIGKAFGFEGQINV
                     PEGDAFADTRIMYDLAEPGRFFGLDDHRGFVVELTDFDVSYFANYTPKDFVSRVRILE
                     DGQEVRRGVTRVNHPLHHDGMKLYQVRFGMAPLVVVRAGGTTLVEEAVMLSDAGGGVW
                     TGALKVSLGATAGERDGPQIALDLVFLPDAAVTEEG"
     gene            complement(1115..1882)
                     /locus_tag="VNU01_11270"
     CDS             complement(1115..1882)
                     /locus_tag="VNU01_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HWH33238.1"
                     /translation="MSVEGLISEANLLIAAAIAFAAGVVSFASPCVVPLVPGYLSYMT
                     GLSGAELREGTRQGAAGRARVLTGGLLFTLGFAVPFTLLGVVGGALSTALTTGAWRVL
                     LGLVVMLLGLAMTGLLPFDVLRREARLTDTAIDRGLLGALPLGFVFGVGWTPCIGPAL
                     GAILTLGVIGSGDNPVRGAMLAFVYALGLGLPFILLGLLFHRAGRALDFLRRNAKSFQ
                     VTGGLLLAGVGLAIATGLWEAFITWLLPAIGRFETVL"
     gene            complement(1879..2442)
                     /locus_tag="VNU01_11275"
     CDS             complement(1879..2442)
                     /locus_tag="VNU01_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWH33239.1"
                     /translation="MRLLPLALACALTLAACTPGSSQESRVGGFVTARGGDFIELDQR
                     EPAPDVAAETLDGDLLALRDLEGPVLVNFWASWCAPCATEAPHLQAVHEAYGPRGLHV
                     VGVDVRDNRPNALSFERDFGVTYPSWYDKAGSIAAAFGGIGPAGLPSTILLDAEHRVA
                     VRLFGAVTGARLAPYLEELVQGQEASP"
     gene            complement(2521..3270)
                     /locus_tag="VNU01_11280"
     CDS             complement(2521..3270)
                     /locus_tag="VNU01_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33240.1"
                     /translation="MSALVLFAAQATPPDSLMAKLAVPLGALIFLGSIYLLLRSNLGT
                     RRGYLVFGAAFFGYMVIQSAFWAFGAPGTPVATGPTNLPGQVANEYQPEWVAFAEDSR
                     LGKGDYAWIQGAEWGAVPEDFADEARVGVDDIQGFFSSDAGHKLVGENWKADTVEYAQ
                     QDGTYPVLRVTYLETDKTGAVAPGGDEVTLYGFYDAGSPSFPAVVLLLISLAGLAVHG
                     LLLDRDEQREKRELRELRGETAAREPVAADA"
     gene            complement(3286..4218)
                     /locus_tag="VNU01_11285"
     CDS             complement(3286..4218)
                     /locus_tag="VNU01_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWH33241.1"
                     /translation="MSNTTLATGILALAALAVIVFLSVSANRKRKAIEDVPPAMRPAY
                     SDEQLETSIFERYQAWGLIMTLFFAVFFPLYYFNESNRLNAEAQEFFVASVVRGEESY
                     KNLCANCHGTDGGGGAAPSPTGEGFWPAPNLLNIVKRYEDNRNVKDIELFIRSTIERG
                     RPGTPMPTWGAGFGGSLNDQQVDDLVNWILASQIDEVDEATAASNLSGEQLFSDNCAK
                     CHGADLQGDVGPALTSVFSRHSEATILEILRNGIIVPTGANMPPWQQGYMYAGARYTD
                     DALERILEYLRSRQTEAPADDTGEDEPQPAPTTA"
     gene            complement(4268..5089)
                     /locus_tag="VNU01_11290"
     CDS             complement(4268..5089)
                     /locus_tag="VNU01_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWH33242.1"
                     /translation="MTTILLLATTAGLAAIAAWWIRRTNLAVAPAGRPVREPRPLAQG
                     CELGSQLLMAASDAGEPAAAGQPGSAATAPAPDTPGPAVHKKRERKPRDLPSRREFLR
                     VASWGAWMATLGGFGGASLMFLWPNLRGGFGARLDVGTEEEILADIRANKAPYEFPAG
                     RTYFVEYSEDLDPDGQYAEITGGAKVMALYWRCVHLGCKVPWCQTSQWFECPCHGSRY
                     NRWGEWQGGPAPRGLDRFPVFIEDGRLIVDTGQLKEGPPRQAPSLQQPQEGPSCL"
     gene            complement(5108..5953)
                     /locus_tag="VNU01_11295"
     CDS             complement(5108..5953)
                     /locus_tag="VNU01_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33243.1"
                     /translation="MSEEVEMDQALYEQLLAEGKSERIARAKAKAAAVRAAKAASGEA
                     TGPRPAGDGEPAPANGDGGAAAPAAAKAPVAVGARAGGGGALTPEERQARVAAALAAK
                     GGNGGVKTETRLAGQEHTHRLLAMVPPTGIQQIRGKQEDKVYTFPHLITAEFVALLAM
                     TVVLVVWSATIPAPLLDLANPNVTPNPSKAPWYFMGLQELLRYFHPMIAGVTVPTVGI
                     IVGMMLPYIDKNPSVKPENRKTAYTLFTIALIFNAVLVIVGSFMRGPGFNWVWPWVDG
                     MWFGL"
     gene            complement(5966..6733)
                     /gene="extP"
                     /locus_tag="VNU01_11300"
     CDS             complement(5966..6733)
                     /gene="extP"
                     /locus_tag="VNU01_11300"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HWH33244.1"
                     /translation="MSKLPSPDAMKAKLQDNVVVKSIFRPGSIYRKGYRDTSRDRALA
                     AMNNVLYHLHPVKVKRHGLKLTYTYCLGGLSFFLFIMLTITGIFLMFFYRPTAGAGAE
                     LAYLDMQNLRQVVTFGDLVRNMHRWAAHLMVFSVFLHMARVFYHGAYKPPREFNWVVG
                     IILLVLTLLLSFTGYLLPWDQLAMWAVAVGTSMAGFTPVFGQQVQFVLLGGVVIGPET
                     LIRWYVLHVLMLPFVLTIFLAVHFWRIRKDGGISGPL"
     gene            complement(6759..7103)
                     /locus_tag="VNU01_11305"
     CDS             complement(6759..7103)
                     /locus_tag="VNU01_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWH33245.1"
                     /translation="MGKTDQGLHPEIFDDYRIETVDANWLQERVKPKRHIGLTPELCI
                     LCRACEDVCPWECIFMMAPSIVTDAENPNVMTLANTAEAIFVIDDNECTRCAICVERC
                     PSDALWLGRVDN"
     gene            complement(7137..7610)
                     /locus_tag="VNU01_11310"
     CDS             complement(7137..7610)
                     /locus_tag="VNU01_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33246.1"
                     /translation="MDWLSEMFRTLPEVGSQFWDFLNDGGVNGVAVTVASVILTAGLL
                     FAAKALRAEHGWLSALFGSMAATIAMFWVFGVIPSAWVYFIDGSRDVLEDKVIPTSAG
                     PQLQNLYQVIRDSVVMGMTGVGIVGLAAVCARIQKEYPRALAEGEEARPQSGGYK"
     gene            complement(7779..>8109)
                     /locus_tag="VNU01_11315"
     CDS             complement(7779..>8109)
                     /locus_tag="VNU01_11315"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWH33247.1"
                     /translation="CIREMPLLARAAADLDGEVAFLGVDVQDQDAKAMRLAREAGVAY
                     PLVRDPAGRLFTSLHGAGMPTTLFVDADGTVVYRRTGEVDAERFRELLREHLGVLWTP
                     PAPAQDG"
BASE COUNT         1221 a         2867 c         2905 g         1116 t
ORIGIN      
        1 atgccctcct cggtgaccgc ggcgtccggc aggaacacca ggtccaacgc gatctgcggg
       61 ccgtcccgct cccccgccgt ggcccccagc gacaccttca gcgctccggt ccacacgccg
      121 ccgccggcgt cggacagcat gacggcctcc tcgaccagcg tggtgcctcc ggcgcgcacc
      181 accacaaggg gcgccatgcc gaagcgcacc tggtacagct tcatgccgtc gtggtgcagg
      241 ggatggttga cgcgcgtcac gccgcgccgc acctcctggc cgtcctccag gatgcgcacg
      301 cgcgagacga agtccttcgg cgtgtagttg gcgaagtagg acacgtcgaa gtcggtgagc
      361 tccaccacga agccgcgatg gtcgtccagg ccgaagaagc ggcccggctc ggcgaggtcg
      421 tacatgatcc gcgtgtcggc gaacgcgtcg ccctccggca cgttgatctg gccctcgaag
      481 ccgaacgcct tgccgatcac ggcgcccgcg agcagcaggt agaaggcggt gtggaagacc
      541 aggctgccgc cctcgcgcca gtgcccgcgc tcggccgcga cctgcgccgc gccggaggcg
      601 gtggcgtcgg cgcaccggcg ccggcggtag ccgcgcagcg ccccggcggc cgcctccagc
      661 gcacgctcgg gcggcagcgc ggtgcgccac tgctcgctgt gcgtgagacg gccgaggttg
      721 cgccccgccg ggggggcctt gcgcacgacg cgcaggaagc cgcgccagcg cggcagcagg
      781 cagccggtga ggctggtgaa cagcagcagc accagggcgg cgaaccaggt cgcgccgaag
      841 acgtcgaaca ggccgagcca gtccagcacc tgggccacgc cggcgcccgg cccggcctca
      901 ccggccagcc agtccgccac ggtgctgggg atcagcggcc gctgcgggat gaacgtcgcg
      961 agcacgctcg cggcggccag caggaacagc agcaccagcg ccgtggacat cttgcgcagc
     1021 cgccgccaca ggcgcagcgc ggtctccccg ggaccgggga tgtcaggcag gcgggacgcc
     1081 accgatggtg gccggtcggg agccatggtc atcgctacag caccgtctcg aagcgcccga
     1141 tcgccggcag cagccaggtg atgaacgcct cccacaggcc cgtggcgatc gcgagcccca
     1201 cgcccgccag cagcaggccg ccggtgacct ggaagctctt ggcgttgcgg cgcaggaagt
     1261 ccagcgcccg ccccgcgcgg tggaacagca ggcccagcag gatgaacggc agccccaggc
     1321 ccagcgcgta gacgaacgcc agcatcgccc cgcgcacggg gttgtcgccc gagccgatga
     1381 cgcccagcgt caggatcgcg ccgagcgcgg ggccgatgca cggcgtccag cccacgccga
     1441 acacgaagcc gagcggcagc gcgcccagca ggccgcggtc gatggccgtg tcggtgaggc
     1501 gcgcctcgcg ccgcagcacg tcgaacggca gcagcccggt catcgccagg ccgagcagca
     1561 tgaccaccag gcccagcagc acgcgccacg cgcccgtggt cagcgccgtg gacagtgcgc
     1621 cgcccaccac gccgagcagc gtgaacggga ccgcgaagcc gagggtgaac agcagcccgc
     1681 cggtcagcac gcgggcgcgg ccggccgcgc cctgccgggt gccctcgcgc agttccgcgc
     1741 cggacaggcc cgtcatgtac gacaggtagc cgggcaccag cggcaccacg cacggcgagg
     1801 cgaaggacac gacgccggcg gcgaacgcga tcgccgcggc gatcagcagg ttggcctcgg
     1861 agatcagccc ctcgacgctc acggcgacgc ctcctgcccc tggaccagct cctccaggta
     1921 gggagccagc cgcgcgccgg tcacggcgcc gaacaggcgg accgcgacgc ggtgttccgc
     1981 gtcgagcagg atcgtggagg gcagcccggc ggggccgatg ccgccgaacg cggccgcgat
     2041 ggaccccgcc ttgtcgtacc aggacgggta ggtgaccccg aagtcccgct cgaagctgag
     2101 cgcgttcggc cggttgtcgc gcacgtccac gccgaccacg tgcaggcccc gcgggccgta
     2161 cgcctcgtgc accgcctgca ggtgcggcgc ctccgtggcg cacggcgcgc accacgacgc
     2221 ccagaagttc accagcacgg ggccttccag gtcgcgcagc gcgagcaggt cgccgtccag
     2281 cgtctccgcg gccacgtcgg gggccggctc gcgctggtcc agctcgatga agtcgccgcc
     2341 gcgggcggtg acgaagccgc ccacacggga ctcctgcgag gagccgggag tgcaggccgc
     2401 gagcgtcagc gcgcaggcca gcgcgagcgg gagaaggcgc atggggccag ggtagcgtca
     2461 aacgaagaag cccccggacg ggccgggggc ttgttcatgc tgcgtggagc ggtcgccggc
     2521 tcacgcgtcg gcggcgaccg gttcccgcgc ggccgtctcg ccgcgcagct cgcgcaactc
     2581 gcgcttctcg cgctgctcgt cgcggtccag cagcaggccg tgcaccgcca ggccggccag
     2641 ggagatcagc agcagcacca cggcggggaa gctcgggctg cccgcgtcgt agaagccgta
     2701 cagcgtgacc tcgtcgccgc cgggcgccac ggcgccggtc ttgtcggtct ccaggtacgt
     2761 gacgcgcagg accgggtagg tgccgtcctg ctgggcgtac tcgaccgtgt cggccttcca
     2821 gttctccccg accagcttgt gaccggcgtc gctggagaag aagccctgga tgtcgtccac
     2881 gcccacgcgc gcctcgtcgg cgaagtcctc cggcaccgcg ccccactcgg cgccctggat
     2941 ccacgcgtag tcgcccttgc ccaggcggct gtcctcggcg aaggcgaccc attccggctg
     3001 gtactcgttg gcgacctggc ccggcaggtt cgtgggaccg gtggccacgg gcgtgcccgg
     3061 cgccccgaag gcccagaacg ccgactggat caccatgtag ccgaagaagg cggcgccgaa
     3121 gaccaggtag ccgcggcgcg tgccgaggtt ggagcgcagc agcaggtaga tgctgcccag
     3181 gaagatcagc gcgcccaggg gcacggcgag cttggccatg aggctgtcag gcggcgtggc
     3241 ctgagcggcg aagagcacga gagcagacat ggtcgccatc cttcgttacg ccgtcgtcgg
     3301 ggcgggctgc ggctcgtcct cgccggtgtc gtccgccgga gcctccgtct ggcgcgagcg
     3361 caggtattcc aggatgcgct cgagcgcgtc gtcggtgtag cgggcgccgg cgtacatgta
     3421 gccctgctgc cacggcggca tgttggcgcc ggtggggacg atgatgccgt tgcgcaggat
     3481 ctccaggatc gtcgcctcgc tgtggcgcga gaacaccgac gtcagcgccg ggccgacgtc
     3541 gccctgcagg tccgcgccgt ggcacttggc gcagttgtcg gagaacagct gctcgccgct
     3601 caggttggac gccgcggtgg cctcgtcgac ctcgtcgatc tggctggcga ggatccagtt
     3661 caccaggtcg tcgacctgct ggtcgttgag cgagccgccg aagccggcgc cccaggtcgg
     3721 catgggcgtg cccggacggc cgcgctcgat ggtcgagcgg atgaacagct cgatgtcctt
     3781 gacgttgcgg ttgtcctcgt agcgcttgac gatgttcagc aggttcgggg ctggccagaa
     3841 gccctcgccg gtgggcgacg gcgcggcacc gccgccaccg tcggtgccgt ggcagttcgc
     3901 gcagaggttc ttgtacgact cctcgccgcg gaccacggag gcgacgaaga actcctgcgc
     3961 ctcggcgttc aggcggttgg actcgttgaa gtagtacagc gggaagaaca cggcgaagaa
     4021 cagcgtcatg atcaggcccc aggcctgata gcgctcgaag atgctggtct ccagctgctc
     4081 gtcggagtac gccggccgca tggcgggcgg cacgtcctcg atcgccttgc gcttcctgtt
     4141 ggcgctgacg gagaggaaca cgatcacggc caaggccgcg agcgcgagga tccccgtcgc
     4201 aagcgtcgtg ttgctcactg gtgttcgccc ttgctgtcga aggtgtgccc ggcttgccga
     4261 tccgccccta caggcagctc ggcccctcct gcggctgctg cagggagggc gcctggcgcg
     4321 gcggtccttc cttcagctgg ccggtgtcca cgatgagtcg cccgtcctcg atgaagacgg
     4381 ggaagcggtc caggccgcga ggcgccgggc cgccctgcca ctcgccccag cggttgtagc
     4441 gggagccgtg gcaggggcac tcgaaccact gcgaggtctg gcaccacggc accttgcagc
     4501 ccaggtgcac gcagcgccag tacagggcca tcaccttggc gccgccggtg atctccgcgt
     4561 actgcccgtc cgggtccagg tcctcgctgt actccacgaa gtacgtgcgg ccggcgggga
     4621 actcgtaggg cgccttgttc gcgcggatgt cggcgaggat ctcctcctcg gtgcccacgt
     4681 ccaggcgcgc gccgaagccg ccgcgcaggt tgggccacaa aaacatcagc gacgcgccgc
     4741 cgaagccgcc gagcgtggcc atccacgcgc cccacgacgc cacgcgcagg aactcgcggc
     4801 gcgacggcag gtcgcggggc ttgcgctcgc gcttcttgtg caccgcgggc ccgggggtgt
     4861 ccggcgccgg cgcggtcgcg gccgagcccg gctggccggc ggcggcgggc tcgccggcgt
     4921 cgctggccgc catcagcaac tggctgccga gctcgcagcc ctgcgccagc ggccggggct
     4981 cgcgcaccgg gcgccccgcc ggggcgacgg ccaggttggt gcgccggatc caccacgcgg
     5041 cgatcgccgc gaggccggcc gtggtggcca ggagcaggat ggtcgtcatg ctcacgttct
     5101 cgcttcctta aagaccgaac cacatgccgt ccacccacgg ccagacccag ttgaagccgg
     5161 ggccgcgcat gaacgaaccg acgatgacga gcaccgcgtt gaagatcagc gcgatggtga
     5221 acaaggtgta ggcggtcttg cggttctccg gcttcaccga cgggttcttg tcgatgtagg
     5281 gcagcatcat cccgacgatg atgccgaccg tggggacggt cacaccggcg atcatcgggt
     5341 ggaagtagcg cagcagctcc tgcaggccca tgaagtacca gggcgccttg gaggggttcg
     5401 gcgtgacgtt ggggttggcc aggtccagca gcggcgcggg gatggtcgcc gaccacacca
     5461 ccaggaccac cgtcatcgcc agcagggcga cgaactcggc ggtgatcagg tgggggaacg
     5521 tgtagacctt gtcctcctgc ttgccgcgga tctgctggat gcccgtgggc gggaccatgg
     5581 ccagcagccg gtgcgtgtgc tcctggccgg ccaggcgggt ctcggtcttc acgccgccgt
     5641 tgccgccctt ggcggcgagc gccgccgcca cgcgcgcctg gcgctcctcc ggggtgagcg
     5701 cgccgccacc gcccgcgcgg gcgccgacgg ccacgggagc cttggcggcg gccggcgcgg
     5761 ccgcgccgcc gtcgccgttc gcgggcgcgg gctcgccgtc tccggccggg cgcgggccgg
     5821 tggcctcgcc cgaggcggcc ttggcggcgc gcaccgcagc ggccttggcc ttcgcgcgcg
     5881 cgatgcgctc gctcttgccc tcggcgagca gctgctcgta gagggcttgg tccatctcca
     5941 cttcttcgga catgggtacg gctccttaca gcgggccgga gatgccgccg tccttgcgga
     6001 tccgccagaa gtggaccgcg aggaagatgg tcaggacgaa cgggagcatg agcacgtgca
     6061 gcacgtacca gcggatcagt gtctcggggc cgatcaccac gccgcccagc agcacgaact
     6121 gcacctgctg gccgaacacc ggggtgaagc cggccatcga ggtgcccacg gccacggccc
     6181 acatcgccag ctggtcccag ggcagcaggt agccggtgaa gctcagcagc agcgtcagca
     6241 ccagcaggat gatgccgacc acccagttga actcccgggg gggcttgtag gcgccgtggt
     6301 agaagacgcg cgccatgtgc aggaacacgc tgaacaccat gaggtgggcg gcccagcggt
     6361 gcatgttgcg gacgaggtcg ccgaaggtca ccacctggcg caggttctgc atgtccaggt
     6421 aggccagctc ggcgccggcg ccggcggtcg gccggtagaa gaacatcagg aagatgccgg
     6481 tgatggtgag catgatgaac aggaagaagc tcaggccacc caggcagtag gtgtaggtga
     6541 gcttcagccc gtgccgcttc accttcaccg ggtgcaggtg gtacagcacg ttgttcatcg
     6601 ccgcgagcgc ccggtcacgg gacgtgtcgc ggtagccctt ccggtagatc gacccgggtc
     6661 ggaagatcga cttcacgacg acgttgtcct gaagcttcgc cttcatcgcg tccggggacg
     6721 ggagcttgct cacctactcg ccctctcttg caaccgtgtc agttgtcgac ccggccgagc
     6781 cacagggcat cggacgggca gcgctccacg cagatcgcgc agcgcgtgca ttcgttgtcg
     6841 tcgatgacga agatcgcctc agcggtgttg gcgagcgtca tcacgttggg gttctcggcg
     6901 tcggtcacga tgctgggcgc catcatgaag atgcactccc aggggcacac gtcctcgcac
     6961 gcgcggcaga ggatgcacag ctccggcgtg aggccgatgt gccgcttggg cttgacccgc
     7021 tcctggagcc agttcgcgtc caccgtctcg atgcggtagt cgtcgaagat ctccggatgg
     7081 agcccttggt cggtttttcc catgtgccgg tccttcccgc tttgtgctcg tccggcctac
     7141 ttgtacccgc ccgactgcgg tcgcgcttcc tctccctcgg cgagcgcccg cgggtactcc
     7201 ttctggatgc gggcgcacac ggccgcgagg ccgacgatcc cgacgccggt catgcccatg
     7261 accacggagt cgcggatcac ctggtagagg ttctgcagtt gcgggccggc ggacgtcggg
     7321 atgaccttgt cctccagcac gtctcgggag ccgtcgatga agtacaccca cgcggacggg
     7381 atgacaccga agacccagaa catcgcgatg gtcgcggcca tcgagccgaa cagcgcggag
     7441 agccagccgt gctcggcgcg cagcgccttg gcggcgaaca gcagcccggc ggtgaggatc
     7501 acgctggcga ccgtgacggc gacgccgttg acgccaccgt cgttgaggaa gtcccagaac
     7561 tgggaaccca cctccggaag ggtgcggaac atctccgaca gccagtccac tccctcgacc
     7621 cttcgtccgt gtcgttcgtg tgcagcgacc ttgaggagcg cgagggcgac aagtggatcc
     7681 ggccagccct cccgcgggca cgcatgctat cccaggcacg tgagaaaccg ccaacgccag
     7741 gcggttttcg cggggatccg gcgggcctgc ggggccgctc agccgtcctg cgccggggcc
     7801 ggaggcgtcc acaggacgcc gagatgctcg cgcaggagct cccggaagcg ctcggcgtcg
     7861 acctccccgg tgcgccggta caccaccgtg ccgtcggcgt cgacgaacag cgtggtgggc
     7921 atgccggcgc cgtgcaggct ggtgaacagg cggccggcgg ggtcgcgcac cagcgggtag
     7981 gccacacccg cctcccgtgc caggcgcatc gccttggcgt cctggtcctg cacgtccacg
     8041 cccaggaagg cgacctcgcc gtccaggtcc gccgcggctc gcgccagcaa cggcatctcc
     8101 cggatgcac
//
LOCUS       DATMKC010000387         3625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_227186, whole genome shotgun sequence.
ACCESSION   DATMKC010000387 DATMKC010000000
VERSION     DATMKC010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227186"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VNU01_11320"
     CDS             complement(<1..1099)
                     /locus_tag="VNU01_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWH33248.1"
                     /translation="MAEYVYVMKGLTKVVPPNKEILKGIWLSFLPGAKIGVIGPNGAG
                     KSTLLRVMAGVDTEFEGEAFAGQGIRVGYLPQEPQLDESKDVKGNIMDGVGETARLLE
                     RFNELSMKMAEPLDDDAMAKVYEEFGQVQDAIEAANAWDLDRTIEVAMDALRVPAGDA
                     DVSKLSGGERRRVALCKLLLSRPDMLLLDEPTNHLDAESVAWLERFLEEYPGTVVAVT
                     HDRYFLDNVAGWILELDRGKGMPFEGNYSGWLEQKSERLRQEEKQESSRQQALARELE
                     WVRASPKARQAKSKARIQSYEALLAKGPQERLGSADIVIPNGQRLGDVVVEADHVSKG
                     FGDRLLMEDLTFKLPPGGIVGVIGPNGAGKTT"
     gene            complement(1138..1965)
                     /locus_tag="VNU01_11325"
     CDS             complement(1138..1965)
                     /locus_tag="VNU01_11325"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWH33249.1"
                     /translation="MRLLATGFALAEAPTLTDDGTLLVSSALGGGLRRLSRDGDTLEL
                     LVPERKGIGGAALDMAGRPIVSGRDVVRLGDGEPAVLLPRGEPVTGFNDIAATPEGGL
                     LAGALHFSPLGGGAPVPGTLRYRAPDGAVSVWHQGIAWPNGIGFSPDGATVYVVDYPG
                     GIVLAGPWDPRRPGALTPLCTSPSGQFDGLAVAADGSLLVALAGGGGVGWFSPDGALR
                     EVLDVPANFVSSVCFAGDGLTDLIVTTLDNTVRPEEGGAVFLAPAPVPGAPVPRYRG"
     gene            complement(1962..3473)
                     /locus_tag="VNU01_11330"
     CDS             complement(1962..3473)
                     /locus_tag="VNU01_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH33250.1"
                     /translation="MPNVLVDLILGAVARHRAAVAFRDAGREATFAQVGKRIARLANA
                     LLAVSPEPGRCVAILMSNRIEYAEADLAAALAGKVKAPVNPRLSDEERAYCLANVQAE
                     TLVTEASELERVAALLPQLPELRRVLVVDAEPGAALPHGAMPYEAALAAASAHASAVD
                     LPDGAPSVVRHTSGTTGRPKGAVISAAARLAAMRTSLIEEIDPAAHDCFLHVAPMGHA
                     SGDKVLTFFARGAANAFLPRFDAGTFADDAEALEATATFLVPTMIRMLLDRGRVPGGF
                     RNVTYGGSLIDPGTRTVAVERFGPVLTQIYGATETPHPVLVLGRDEHLLPETAQATGR
                     PAHGVEVRLAGDGELLVRGANVMSGYWNDPKATAEVLVDGWYHSGDIARQLPGGWYAI
                     VDRKKDLVITGGLNVYPAEVEAALLRHPGVAEAAVFGVPDPLWGEIVAAAVIPARGAT
                     PTVEELQAHCEALLAGYKKPRRIELRDALPKTATNKILKRALRDEATTGRAGR"
BASE COUNT          497 a         1340 c         1280 g          508 t
ORIGIN      
        1 gcgtggtctt gccggcccca ttagggccga tcacgccgac gatgccgccc ggcggcagct
       61 tgaacgtgag gtcctccatc aggaggcggt cgccgaagcc cttgctcacg tggtcggcct
      121 cgaccaccac gtcgcccagt cgctggccgt tggggatcac gatgtccgcg ctgcccagcc
      181 gctcctgcgg gcccttggcc agcagcgcct cgtagctctg gatgcgcgcc ttgctcttgg
      241 cctggcgcgc cttcggggac gcgcgcaccc actcgagctc gcgggccagg gcctgctgcc
      301 gcgaggactc ctgcttctcc tcctggcgca gccgctcgga cttctgctcc agccagccgg
      361 agtagttgcc ctcgaacggc atgcccttgc cgcggtccag ctccaggatc cagccggcca
      421 cgttgtccag gaagtagcgg tcgtgcgtca cggccaccac cgtgcccgga tactcctcca
      481 ggaagcgctc cagccaggcc acggactccg cgtcgaggtg gttggtgggc tcgtccagca
      541 gcagcatgtc ggggcgggac agcagcagct tgcacagcgc gacgcggcgg cgctcaccgc
      601 cggacagctt gctgacgtcg gcgtcgcccg cggggacgcg cagcgcgtcc atcgcgacct
      661 cgatggtgcg gtccaggtcc cacgcgttgg ccgcctcgat ggcgtcctgg acctggccga
      721 actcctcgta gaccttggcc atcgcgtcgt cgtcgagcgg ttcggccatc ttcatggaca
      781 gctcgttgaa gcgctccagc aggcgcgcgg tctcgccgac gccgtccatg atgttgccct
      841 tcacgtcctt ggactcgtcc agctgcggct cctgcggcag gtagccgacg cggatgccct
      901 ggccggcgaa cgcctcgccc tcgaactcgg tgtccacgcc cgccatgaca cgcagcaggg
      961 tcgacttgcc cgcgccgttg gggccgatga cgccgatctt ggcccccgga aggaagctca
     1021 gccagatccc cttgaggatc tccttgttgg ggggcacgac cttggtcagg cccttcatca
     1081 catagacgta ctcagccaca gttgctccag cggaggggtt gggggacgcc gctcatgcta
     1141 gccgcggtag cgcgggacgg gcgcgccggg gacgggcgcg ggggcgagga agacggcacc
     1201 gccctcctcg ggccgcacgg tgttgtccag cgtcgtgacg atcaggtcgg tcaggccgtc
     1261 gccggcgaag cagacgctgc tcacgaagtt cgccggcacg tcgaggacct cgcgcagcgc
     1321 gccgtcgggt gagaaccagc cgacaccgcc cccgcccgcc agggcgacca gcaggctgcc
     1381 gtcggcggcc accgcgaggc cgtcgaactg gccggacggc gaggtgcaca gcggcgtcag
     1441 cgccccgggg cgccgtgggt cccacgggcc ggcgagcacg atcccgcccg gatagtcgac
     1501 gacgtacacc gtggcgccgt cgggcgagaa gccgatgccg ttgggccagg cgatgccctg
     1561 gtgccacacg ctgaccgcac cgtccggtgc ccggtagcgc agcgtccccg ggaccggcgc
     1621 cccgccgccc agagggctga agtgcagcgc acccgcgagc agaccgccct ccggcgtcgc
     1681 ggcgatgtcg ttgaaaccgg tgaccggctc gccccgcggc aggagcaccg ccggctcgcc
     1741 gtcgccgagg cggacgacat cgcggccgga cacgatcggc cgccccgcca tgtccagcgc
     1801 cgcgccgccg atgcccttgc gctctggcac cagcagctcg agcgtgtcgc cgtctcggct
     1861 cagccgccgc agcccgccgc ccaaggcgct gctcaccagc agcgtgccgt cgtcggtgag
     1921 cgtcggcgcc tccgcgagcg cgaaccccgt cgccagcagc ctcatcgccc cgccctcccc
     1981 gtcgtggcct cgtcgcgcag cgcgcgcttg aggatcttgt tcgtcgccgt cttgggcaac
     2041 gcatcgcgca gctcgatgcg gcgcggcttc ttgtagcccg cgagcagcgc ctcgcagtgc
     2101 gcctggagct cctcgacggt cggggtggcg ccgcgcgcag ggatgacggc cgccgccacg
     2161 atctcgcccc acagcggatc cggcacgccg aacaccgcgg cctcggccac gcccgggtgg
     2221 cgcagcaacg ccgcctcgac ctcggcgggg tacacgttca gcccacccgt gatgacaagg
     2281 tccttcttgc ggtccacgat cgcgtaccag ccgcccggga gctgacgcgc gatgtcgccg
     2341 gagtggtacc agccgtccac gagcacctcg gcggtggcct tcgggtcgtt ccagtagccg
     2401 ctcatcacgt tggcgccgcg caccagcagc tcgccgtcgc cggccagccg cacctcgacg
     2461 ccgtgcgccg ggcggcccgt cgcctgcgcc gtctcgggca gcagatgctc gtcacggccc
     2521 aggacgagca cggggtgcgg cgtctccgtg gcgccgtaga tctgcgtgag cacgggcccg
     2581 aagcgctcga ccgccacagt gcgggtccca gggtcgatga gcgagccgcc gtaggtgacg
     2641 ttgcggaagc cgcccggaac ccgcccgcgg tccagcagca tgcggatcat cgtgggcacc
     2701 aggaacgtgg cggtggcctc cagcgcctcg gcgtcgtccg cgaaggtccc ggcgtcgaag
     2761 cgcggcagga acgcgttggc cgcgccgcgc gcgaagaacg tgagcacctt gtcgccggag
     2821 gcgtggccca tcggcgcgac gtgcaggaag cagtcgtgcg cggccgggtc gatctcctcg
     2881 atcagcgacg tgcgcatggc ggcgaggcgc gccgcggcgg agatgacggc gcccttgggg
     2941 cggccggtgg tgccggaggt atgacggacc accgacggcg ctccatcggg aagatcgacc
     3001 gcggacgcgt gcgcggaagc ggcggcgagc gcggcctcgt agggcatcgc gccgtgcggc
     3061 agcgcggcgc caggctcggc gtcgacgacc agcacgcgcc gcaactccgg tagttggggc
     3121 aggagcgcgg cgacacgctc cagctcggac gcctcggtga ccagcgtctc ggcctgcacg
     3181 ttggccaggc agtacgcgcg ctcctcgtcg gaaaggcgtg ggttgaccgg cgccttgacc
     3241 ttgccggcca gggcggcggc caggtccgcc tcggcgtact cgatgcggtt gctcatcagg
     3301 atcgcgacgc aacggcccgg ctcgggcgac accgcgagca gcgcattggc cagccgcgcg
     3361 atgcgcttcc ccacctgggc gaacgtggcc tcgcgcccgg cgtcgcggaa tgcgaccgcg
     3421 gcacgatgcc gcgccaccgc cccgaggatc aggtcgacca gaacgttggg caccggcgca
     3481 gcctaccgac ccgcgttgtg aaccgtttcc ctcacatagc agggaaaccg cccactgtgg
     3541 gcggtttccc tgctatgtga gggaaacggt tcacaacgcg ggtcggtagg ctgcgccggt
     3601 gcccaacgtt ctggtcgacc tgatc
//
LOCUS       DATMKC010000388         3990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_195872, whole genome shotgun sequence.
ACCESSION   DATMKC010000388 DATMKC010000000
VERSION     DATMKC010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195872"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VNU01_11335"
     CDS             <1..450
                     /locus_tag="VNU01_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33251.1"
                     /translation="VLLTTQYLEEADRLADRIGVIHRGRIVREGTADELKVDVGGAVL
                     ELAVAEESRPAAMAALERLSGEEPLYDEATGRLRAPAPDGAASMLAAVRALDAVGVEP
                     VDVALHKPTLDDVFLALTGDAPGSDETGDDDAAPGSGQAASTEAKIR"
     gene            447..1283
                     /locus_tag="VNU01_11340"
     CDS             447..1283
                     /locus_tag="VNU01_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33252.1"
                     /translation="MTASTVPVRTRTTVASTVSDIAVLMRRNLLRTVRMPQLMVFSTV
                     QPVMFLLLFNYVFGGAIGQAIPPAAGGKYINWLMPGLLVQISTFGAGQTAIGLTEDLA
                     KGVVDRFRSLPIARSAVLAGRTLADVVRNSFVLALMLAVGFLIGFRPQTDVPRFLAGF
                     GMVMLFTFSLSWVMATVGLKVRSPEAAQSAAFIPIFPLVFASSVFVPTQTMPDWLRAF
                     ADHQPITVITNALRGLMLGEGALLPGQTVGGTVVAALAWMAGISVVFGFLAVRTYRKA
                     VL"
     gene            1335..2141
                     /gene="prcB"
                     /locus_tag="VNU01_11345"
     CDS             1335..2141
                     /gene="prcB"
                     /locus_tag="VNU01_11345"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HWH33253.1"
                     /translation="MKLSGTDPFDLAGPSFFALLQREAPEQAARVLTSAQSSGMVDHV
                     DGTTVIALTYGAGGDQGVIMAGDRRATSGNIISKRDMRKVFEADSWSAVGISGAAGPA
                     MELAKIFATELEHYEKVEGNPLSLEGKANKLAQMVRANLPLAMQGLVVVPLFAGYDPR
                     EGVARIYEFDAAGGRYEERRFATTGSGGRDARGSLKARFREDLVREGAIDVAIEALWD
                     AADEDSATGGPDLVREIYPLVAVIDSGGYRELADDVVAERVRGVIDRRRQ"
     gene            2170..2844
                     /gene="prcA"
                     /locus_tag="VNU01_11350"
     CDS             2170..2844
                     /gene="prcA"
                     /locus_tag="VNU01_11350"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HWH33254.1"
                     /translation="MAMPYYVSPEQVMKDKADFARKGIARGKSVAALTYADGILFVAE
                     NPSATLHKISEIYDRIAFAAVGKYNEFEQLRVAGIRLADVKGYQYAREDVTGRALANA
                     YATALGAAFTESSKPFEVELLVAEISADSVELYHITYDGTIMDREGHVAIGGSSDAIT
                     AALDSRFDASLPRDEALRVARDALSTAEERPIDADRLEVAGLDRTRGRRKFFRMSGTE
                     IAAALG"
     gene            complement(2861..3172)
                     /locus_tag="VNU01_11355"
     CDS             complement(2861..3172)
                     /locus_tag="VNU01_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33255.1"
                     /translation="MTRALPTWFATALAGAGVVAGALSLEGLSTAQYLALQLGGLAVA
                     AGMFAWIVRSHARAASATSGQVLTVVLLALAMPVMALRALTVLFLLASGQPLAAGPFS
                     G"
     gene            3410..>3990
                     /locus_tag="VNU01_11360"
     CDS             3410..>3990
                     /locus_tag="VNU01_11360"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWH33256.1"
                     /translation="MERRIFGIEVEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSVRQVVAHDKAGERILEGLVEQAELRLHEEGI
                     AGQIYLFKNNTDSAGNSYGCHENYLTSRRDDFGHYAEVLIPFLVSRQIYAGAGKVLQT
                     ARGAMYCVSQRAEHIWEGVSSATTRSRPIINTR"
BASE COUNT          591 a         1433 c         1422 g          544 t
ORIGIN      
        1 gtgctgctca ccacgcagta cctggaggag gcggaccggc tcgccgaccg catcggcgtg
       61 atccaccgcg gccgcatcgt gcgggaaggc accgccgacg agctcaaggt ggacgtgggc
      121 ggcgcggtgc tggagctggc cgtggccgag gagtcccgcc cggccgcgat ggcggcgctg
      181 gagcgcctga gcggcgaaga gccgctgtac gacgaggcga ccgggcggct gcgcgccccc
      241 gcgcccgacg gcgcggccag catgctggcg gccgtgcgcg cgctggacgc cgtcggggtg
      301 gagccggtcg acgtggcgct gcacaagccg accctggacg acgtgttcct ggcgctgacc
      361 ggcgacgcgc ccggcagcga cgagaccggc gacgacgacg cggcacccgg ctccggccag
      421 gccgcgtcca cggaggcgaa gatccgatga ccgcgagcac cgtgcccgtg cgcacgcgca
      481 ccaccgtggc gtccaccgtg tccgacatcg ccgtgctcat gcggcgcaac ctgctgcgca
      541 ccgtgcgcat gccgcagctg atggtgttct ccacggtgca gcccgtcatg ttcctgctgc
      601 tgttcaacta cgtcttcggc ggcgccatcg gccaggcgat cccgcccgcg gcgggcggca
      661 agtacatcaa ctggctgatg cccggcctgc tggtgcagat ctccacgttc ggcgccgggc
      721 agaccgccat cggcctcacc gaggatctcg ccaagggcgt ggtggaccgc ttccgctcgc
      781 tgcccatcgc gcggtcggcc gtgctggcgg gacgcacgct cgccgacgtg gtgcgcaaca
      841 gcttcgtgct cgcgctcatg ctcgccgtcg gcttcctgat cggcttccgc ccgcagacgg
      901 acgtgccgcg cttcctggcc ggcttcggca tggtgatgct gttcacgttc tcgctgtcgt
      961 gggtgatggc gaccgtgggc ctcaaggtgc gcagccccga ggcggcgcag tccgcggcgt
     1021 tcatcccgat cttcccgctg gtgttcgcct cgtcggtgtt cgtgcccacc cagaccatgc
     1081 cggactggct gcgcgccttc gccgaccacc agcccatcac cgtcatcacc aacgccctgc
     1141 gcgggctgat gctgggggag ggggcgctgc tgccgggcca gacggttggc ggcaccgtgg
     1201 tggccgcgct ggcgtggatg gccgggatca gcgtggtgtt cgggttcctg gcggtgcgca
     1261 cgtaccgcaa ggcggtgctg tgagagccgg acgctaggat gcctgctccg tggtgaccga
     1321 tcgaaggacg tggagtgaag ctttccggca ctgacccgtt cgacctcgcg ggacccagct
     1381 tcttcgccct gctccagcgc gaggcgccgg agcaggcggc gcgggtgctg acgagcgcgc
     1441 agtcctcggg catggtggac cacgtggacg gcacgacggt catcgcgctc acctacggcg
     1501 ccggcggcga ccagggcgtc atcatggccg gcgaccgccg cgcgacgtcc ggcaacatca
     1561 tctccaagcg cgacatgcgc aaggtgttcg aggccgactc gtggtcggcg gtgggcatct
     1621 ccggcgcggc cgggccggcc atggagctcg cgaagatctt cgccacggag ctggagcact
     1681 acgagaaggt cgagggcaac ccgctgagcc ttgagggcaa ggcgaacaag ctcgcccaga
     1741 tggtgcgcgc caacctgccg ctggcgatgc aggggctggt ggtggtgccg ctgttcgccg
     1801 gctacgaccc gcgcgagggc gtcgcccgga tctacgagtt cgacgccgca ggcggccgct
     1861 acgaggagcg ccgcttcgcc accaccggct ccggcggccg tgacgcgcgc ggctcgctca
     1921 aggcgcgctt ccgcgaggac ctggtccgcg agggcgccat cgacgtggcg atcgaggcgc
     1981 tgtgggacgc cgccgacgag gactccgcaa ccggcgggcc ggacctggtc cgcgagatct
     2041 acccgctggt ggcggtcatc gactcgggcg gctaccgcga gctggccgac gacgtggtcg
     2101 ccgagcgcgt gcgcggcgtc atcgaccggc gccgccagta gcgaccgccc gcaggcgagg
     2161 agacgaccca tggccatgcc gtactacgtg tcacccgagc aggtgatgaa ggacaaggcg
     2221 gacttcgccc gcaagggcat cgcccgcggc aagtccgtcg ccgcgctcac ctacgccgac
     2281 ggcatcctgt tcgtggcgga gaacccctcg gcgacgctgc acaagatcag tgagatctac
     2341 gaccgcatcg ccttcgccgc cgtcggcaag tacaacgagt tcgagcagct gcgcgtcgcc
     2401 gggatccgcc tggccgacgt gaagggctac cagtacgcgc gcgaggacgt caccgggcgg
     2461 gcgctggcca acgcctacgc caccgccctc ggcgcggcgt tcaccgagtc gtccaagccg
     2521 ttcgaggtgg agctgctggt cgccgagatc agcgccgaca gcgtggagct gtaccacatc
     2581 acctacgacg gcacgatcat ggaccgtgag ggccacgtgg ccatcggcgg ctcctccgac
     2641 gcgatcaccg cggcgctgga cagccgcttc gacgcctcgc tgccgcgcga cgaggcgctg
     2701 cgcgtggcgc gggacgccct gtccacggcg gaggagcgcc ccatcgacgc cgatcgcctg
     2761 gaggtggccg gactggaccg cacgcgggga cggcgcaagt tcttccgcat gagcggcacc
     2821 gagatcgccg ccgcactggg ctgagcggcc gcggcgccgg tcagccgctg aaggggccgg
     2881 ccgccagcgg ctggccgctc gccagcagga acagcacggt gagcgcgcgc agcgccatca
     2941 ccggcatcgc cagggccagc aggaccaccg tgaggacctg cccggacgtc gccgaggcgg
     3001 cccgcgcgtg actgcgcacg atccacgcga acatgccggc cgccacggcc aggccgccga
     3061 gctgcagcgc caggtactgc gccgtgctca agccctccag cgacagcgcg ccggccacca
     3121 cgcccgcgcc cgcgagggcg gtggcgaacc aggtcggcag ggcgcgcgtc atacgcagtg
     3181 catcggccgc tgacgccccc gagttgacgg ccgaatgccg tccgcacctt gtccacgggg
     3241 acgagcccag ctcgggcccg tcgctgggcc ttcgccgttc cgttcgcgga cgtgggcccg
     3301 cccgcctgag ccacccgcaa ggtttgggcg catccggccg actggtacca cggcgaccta
     3361 caatcgcggg acggccacat cgcggggcca gcgtcggaag gcaggaccca tggaacgccg
     3421 gatcttcggc atcgaagtcg agtacggcgt cacgtgcacg ttccggggac agcgcaggct
     3481 gtcgccggac gaggtcgcgc gctacctgtt ccgccgcgtc gtgagctggg gccgctccag
     3541 caacgtgttc ctggagaacg gcgcgcgcct gtacctcgac gtcggcagcc accccgagta
     3601 cgccaccccc gaatgcgact ccgtgcgcca ggtcgtcgcc cacgacaaag cgggcgagcg
     3661 catcctcgag ggcctcgtcg agcaggccga gctgcgcctg cacgaggagg gcatcgccgg
     3721 gcagatctac ctgttcaaga acaacaccga ctcggcgggc aactcctacg gctgtcacga
     3781 gaactacctg accagtcgcc gtgacgattt cggccactac gccgaggtgc tgattccctt
     3841 cctggtctcg cgccagatct acgccggagc ggggaaagtg ctccagacgg cccggggggc
     3901 catgtactgc gtctcgcagc gggccgaaca catctgggag ggtgtctcca gcgcgaccac
     3961 ccgcagccga cctatcatca atacccgcga
//
LOCUS       DATMKC010000389         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_256221, whole genome shotgun sequence.
ACCESSION   DATMKC010000389 DATMKC010000000
VERSION     DATMKC010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256221"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1197
                     /locus_tag="VNU01_11365"
     CDS             82..1197
                     /locus_tag="VNU01_11365"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HWH33257.1"
                     /translation="MGAVHWRELYREVVDTGLCTGCAACVMACPRDVLDYIPATYAPV
                     QVGEGMAVDECFHGDRGCDICTRACPRFRAWESELDTALFGRERTPEESWGIAKSVLL
                     VRATDSEVFTAGQDGGLVSALLIWGLETGRIDGALASKLTEGRPFDAEPALVTSREQV
                     LATAGSRYTYSANPLAMKQAEAEGLRNLALVGMSCMSSINGTVLARGIKKYANRIALT
                     VGLLCSKTFTYEGQEEVLAEHGIAVSDVVKVNIKGRYMVWTRDGGYTEIPLKELHPHT
                     RPGCKTCPDFAAEHADISTGGIGADDDWTLTLVRTARGEEWIQGLLDAGLIEAKPGET
                     DEVAMNLLDKLSRVSRKRWPADLLPAGHRAPALLPVV"
     gene            1202..>2511
                     /gene="cofG"
                     /locus_tag="VNU01_11370"
     CDS             1202..>2511
                     /gene="cofG"
                     /locus_tag="VNU01_11370"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HWH33258.1"
                     /translation="MSRFPHALADDLPSGVRRALARADDGKTLSVDEAAWLLSARGAA
                     LEALMGIAARVRDGAFGARVTYSRKVFVPLTHLCRDSCGYCTFAWPPKGDLPAFLSPE
                     QVLEIARAGQEAGCKEALFTLGDKPELRYPAAREWLAARGYDSTLEYLRAVAIQVIEE
                     TGLVPHLNPGVMSWTELSMLKQVSGSMGIMLESASLRLLAKGQAHWHAPDKDPEVRLR
                     TIEDAGRLAIPFTSGVLIGIGETERERAEALFALAGSARRYGHVQEVIVQNFRAKPDT
                     AMRDAPEPSADELLATIAVARLVLPVKVHLQAPPNLSPSLEARMVAAGIDDFGGVSPV
                     TPDHVNPEYPWPHLDGLAARVAEAGHELRERLCVYPEFVRRPDPWLAGRMRGPVLALA
                     DDEGLAVAGLSPTGHDWQDPETPVGAPVGSDARWTSAVAGDGVTG"
BASE COUNT          335 a          910 c          935 g          331 t
ORIGIN      
        1 gcctacccgc gacccgaggc ccttagactg gccgtccggg cgatttcacg ctcctgccca
       61 cgctccggag aaaggcgttg catgggcgcc gtgcactggc gcgagctcta ccgcgaagtg
      121 gtcgacacgg ggctgtgcac cgggtgcgcc gcgtgcgtca tggcctgccc gcgcgacgtg
      181 ctcgactaca tcccggcgac ctacgcgccc gtccaggtgg gtgagggcat ggccgtggac
      241 gagtgcttcc acggcgaccg cggctgcgac atctgcacgc gcgcctgccc gcgcttccgg
      301 gcctgggagt ccgagctgga caccgcgctg ttcggccgcg agcgcacgcc ggaggagagc
      361 tggggcatcg ccaagtccgt gctgctggtg cgcgccaccg acagcgaggt gttcaccgcc
      421 ggccaggacg gcgggctggt gtccgcgctg ctcatctggg gtctggagac cggccgcatc
      481 gacggcgcgc tggcgtccaa gctgaccgag ggccgcccgt tcgacgccga gccggcgctg
      541 gtgacctcgc gcgagcaggt actggccacg gccgggtcgc gctacacgta ctcggccaac
      601 ccgctggcga tgaagcaggc cgaggcggag gggctgcgca acctcgcgct ggtgggcatg
      661 agctgcatgt cgtccatcaa cggcaccgtg ctggcgcgcg gcatcaagaa gtacgccaac
      721 cgcatcgcgc tcaccgtggg cctgctgtgc tccaagacgt tcacgtacga gggccaggag
      781 gaggtgctgg ccgagcacgg catcgccgtg tccgacgtcg tgaaggtcaa catcaagggc
      841 cgctacatgg tgtggacgcg cgacggcggc tacaccgaga tcccgctgaa ggagctgcac
      901 ccgcacacgc gcccgggctg caagacctgc ccggacttcg ccgccgagca cgccgacatc
      961 tccaccggcg gcatcggcgc ggacgacgac tggacgctca ccctggtgcg caccgcccgc
     1021 ggcgaggagt ggatccaggg gctgctggac gccggcctga tcgaggccaa gcccggcgag
     1081 accgacgagg tggcgatgaa cctgctggac aagctgtcgc gcgtgtcgcg caagcgctgg
     1141 cccgccgacc tgctgcccgc cggccaccgc gccccggccc tgctgccggt ggtgtgaccc
     1201 cgtgagccgg ttcccccacg ccctcgccga cgacctgccc agcggcgtgc gcagggcgct
     1261 ggcgcgcgcc gacgacggca agacgctgtc cgtcgacgag gcggcctggc tgctgtcggc
     1321 gcgcggggcg gcgctggagg cactgatggg catcgccgcg cgcgtccgcg acggcgcctt
     1381 cggcgcgcgt gtcacctact cgcgcaaggt gttcgtgccg ctgacgcacc tgtgccggga
     1441 ctcctgcggc tactgcacgt tcgcgtggcc gccgaagggc gacctgcccg ccttcctgtc
     1501 gcccgagcag gtgctggaga tcgcgcgggc cggccaggag gccggctgca aggaggcgct
     1561 gttcaccctc ggcgacaagc cggagctgcg ctacccggcg gcgcgcgagt ggctggccgc
     1621 gcgcggctac gactccacgc tggagtacct gcgggccgtg gcgatccagg tcatcgagga
     1681 gaccggcctg gtgccccacc tcaaccccgg cgtcatgagc tggaccgagc tgtccatgct
     1741 caagcaggtc tccgggtcca tgggcatcat gctggagtcg gcgtcgctgc ggctgctggc
     1801 caagggccag gcccactggc acgcgcccga caaggacccc gaggtgcgcc tgcgcaccat
     1861 cgaggacgcc ggccgcctgg cgatcccgtt cacctccggg gtcttgatcg gcatcggcga
     1921 gaccgagcgc gagcgcgccg aggcgctgtt cgcgctggcc gggtccgcgc gccgctacgg
     1981 gcacgtccag gaagtgatcg tgcagaactt ccgcgccaag ccggacacgg cgatgcgcga
     2041 cgcgcccgag ccgtcggccg acgagctgct cgcgaccatc gccgtggcgc ggctggtgct
     2101 gccggtcaag gtgcacctgc aggcgccgcc gaacctgtcg ccgtcgctgg aggcgcgcat
     2161 ggtcgccgcg ggcatcgacg acttcggcgg cgtgtcgccg gtgaccccgg accacgtcaa
     2221 cccggagtac ccgtggccgc acctggacgg cctggccgcg cgcgtcgccg aggcgggcca
     2281 cgagctgcgc gagcggctgt gcgtgtaccc ggagttcgtg cgccggcccg acccgtggct
     2341 ggccgggcgc atgcgcggac cggtcctggc gctggccgac gacgagggcc tggccgtggc
     2401 gggcctgtcg cccacgggcc acgactggca ggacccggag acgcccgtgg gcgcgccggt
     2461 gggctcggac gcgcgctgga cgtcggccgt ggccggcgac ggcgtcaccg g
//
LOCUS       DATMKC010000390         9837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_223596, whole genome shotgun sequence.
ACCESSION   DATMKC010000390 DATMKC010000000
VERSION     DATMKC010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223596"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VNU01_11375"
     CDS             <1..588
                     /locus_tag="VNU01_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWH33259.1"
                     /translation="IVGCTGGGSNFAGLSFPFLREKLAGKMDPRILAVEPAACPSLTR
                     GVYTYDFGDTAGMTPLVKMHTLGHDFVPEAIHAGGLRYHGMSPLISHIYELGLIEAVA
                     KNQSECFAAGVQFARTEGIVPAPEPTHALAATVEEALRCKETGEEKVVLTALCGHGHF
                     DMAAYDRYLAGELVDYAYPEEKVADALTRLPQVDL"
     gene            complement(638..1948)
                     /locus_tag="VNU01_11380"
     CDS             complement(638..1948)
                     /locus_tag="VNU01_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH33260.1"
                     /translation="MATTRWRMLAILMLVLSLVATACGGGDDDADTDGDGGDGGGSAV
                     GVTDDPCPNAVNADNGCIYLGIISDLTEGPFRALAVPLTDAQKAFWARVNNDGGIGGY
                     DIDVETHIKDNKYIPETHKQVFEEIRGDVLGLAQTLGSPQTAAIIQDLRDDSIVAAPA
                     SWTSAWEFEDVIIESGTNYCMEMANAVDWYAEEQAAPTKVMAIHYPGDYGDDAAAGAR
                     LAAERIGAEFVDHPTQPGQENQSGAVAAIVQEKPDLVVVTTGPTELATIVGGAASQGF
                     TGKVIGSSPTWNPALLQTAAAPALKAMYFQAGPWGPWGTESAGHAALREAFPDTTPND
                     GYVAGWVWQYPLKAALEKAVEDGNLTREGLLEAVKTLETVDYEGMLPEEAGNYAGEPA
                     DAVFRQSVVSQVDDTQPTGVNVVADFFTGPTAEGMAFDKPCFEA"
     gene            complement(1987..3144)
                     /locus_tag="VNU01_11385"
     CDS             complement(1987..3144)
                     /locus_tag="VNU01_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWH33261.1"
                     /translation="MSATTHAPELGELGGRRVRGRPELYTSYRQDQALLNTAPKRAGV
                     TALFLAGVVLPFLVGNDMVLLLATAAAAAIGAIGLNIVTGMAGQVSLGHAFFVGVGAF
                     TAATLAGDPDSSRHIGFGLDMLLWLPGAAVVAGAFGALVSPLAVRLRGLYLAIVTLGL
                     VFLGEHAFKELKDLTGGVGVGRPGPEAVVLGVDLTEPSTLLGLTLTRHVKLYLLMLVL
                     LAVCAVLGRNVGRSALGRAFSAVRDRDIAAAVIGVPLTKYKVLAFTISSAFAGVSGAL
                     LYTIIGHIEPSSFNLLMSIRFIAMILIGGIATVSGAIMGAVFITMLPRMSQFLVRELP
                     FLSGGQLLDQHQIEAVLYGLLIIGFLVFEPRGLFGVWLRVRNYWKGWPFSY"
     gene            complement(3141..4034)
                     /locus_tag="VNU01_11390"
     CDS             complement(3141..4034)
                     /locus_tag="VNU01_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWH33262.1"
                     /translation="MTRLAQAIVVGLGNGSIYALLALGFVIIYKATSVISFAQPSIMV
                     AGAMLTYLFGHVVGLGFFPALALAAAATAVLALVVERTALRPMVGKPVFVVAIITIGV
                     DVVIRVVVNRFIGLDVRPVDNPWGLSTTTFGGVIVQQRHIAMLLTTAVLVAALSLFFK
                     YSRVGLAMRAAALDQETAQAQGVSVGMVFALSWAIAGALAAVAGAFVGTGTGIEGSTW
                     TVALKALPAIILGGLDSVPGAVVGGILIGVVEALVATYQGSYFPWLGANFSVVSPYLV
                     MLLVLLVRPYGLFGTPEVERV"
     gene            complement(4031..5875)
                     /locus_tag="VNU01_11395"
     CDS             complement(4031..5875)
                     /locus_tag="VNU01_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWH33263.1"
                     /translation="MTTTFEAPATTISIATRLRDWAHRDPGGVALREKRLGVWHEITW
                     QAYWDLVQDVAHAFLSLGVEPGDRVAIHSENRPEWVVCDVACVAVRAVSMGVYPTNPA
                     AEVEYLLDNSGAKILVAEDQEQVDKALAVADRLPHLERIVYVDPRGVRRYDDPRLLAW
                     EDLLAMGRRHRAAHAGEVERLMSQARPDDLATLVYTSGTTGPPKGAMLTVANVEFAIT
                     SAVVSGALADPPPGQSDMLLSYLPLAHVAERLFTVWFNLGAGPVVNFAESIETVQQNL
                     REIQPTIFLGVPRIWEKMHAGIEIRMANASRLKRANYRFWVARGLRLVDKDLAGARLT
                     PLDRVVRLLGHLLLYRALKDRIGMRCCRFAASGAAPVAPDLLRFFMAIGVPMHEAYGM
                     TENSAVATSNRPGRVKLGTVGEPYPGAEVRVDPATGEIQTRHPGNFAGYWRNEQATRE
                     TFTEDGWLRTGDVGEWVDGTHLKITDRLKDIIITAGGKNISPSELENALKTSPYIKEA
                     VVLGDRRPYLTALIGIELDTVGDWATRQGIPYTTYRDLSEKPEVLALVRKEIDRTNAR
                     FAQVETIKKFRMLPKELDHEDGELTATQKVKRAAISRQFSDLVEAMYQ"
     gene            complement(5872..6663)
                     /locus_tag="VNU01_11400"
     CDS             complement(5872..6663)
                     /locus_tag="VNU01_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH33264.1"
                     /translation="MSLLDFRDVRLRFAGVTALDGVSFTVQPGELFAIIGPNGAGKTS
                     VFNVLSGIYAPQEGSVRFDGQELVGMAPHRIAQLGMARTFQNIELFENLTVVDNLMLG
                     RHGHVRYGAVSALAYAGRAAREEVRNRAVVEEIIDFLEIEEHRKYPVRLLPYGVQKRV
                     ELGRALAMEPKLLLLDEPVAGMNVEETEDMARFVLDIREELGIPIILVEHDMGVVMDL
                     ADRVMVLDFGKPITTGTPDEVQRHPDVIRAYLGAAAATPGEGGTT"
     gene            complement(6660..7448)
                     /locus_tag="VNU01_11405"
     CDS             complement(6660..7448)
                     /locus_tag="VNU01_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH33265.1"
                     /translation="MVLTVENLEVVYDDVILVLRGVSLEVPDGKIVALLGANGAGKTT
                     LLRAISGLLDVHDGEITKGRISLDGSPIHHLPPARIVRAGVTQVLEGRRIFSEFTVEE
                     NLRIGGYTNAKTAKHNLDRVYELFPVLKDRARSTAGYLSGGEQQMLAMGRALMSDPRY
                     LLLDEPSLGLAPLLVEQIRDLVVEINKQGTTVLLVEQNAHMALSIADHGYVMETGKVV
                     MDNAAAALLEDEDVREFYLGMGTEGAAKSFRDVKHYKRRKRWLS"
     gene            complement(7578..>9837)
                     /locus_tag="VNU01_11410"
     CDS             complement(7578..>9837)
                     /locus_tag="VNU01_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33266.1"
                     /translation="FGMAPAALLGVDVDAVWGHAAELLVAAGPGVPAAANPGVQLAAF
                     MAGLARGGRDKLTLLAPEPLGPLGDWVEQLVAESTGKQGVGVVPVVGEPVGRPGSYGD
                     DRAFVAYRFGDTEPEGARALADAGHPVLFLDVASREHLGAEFLRWELATALAGALLGV
                     NPFDEPNVTEAKEQTRAILAEVAGGGGVPAPEHGDVPALCATLRPGDYLAIQAYLPPS
                     SATLASFAQARAAARDLRGIATTFGWGPRFLHSTGQLHKGGADNVVVLQIVDATLWQD
                     AAPVDIPGRPYDFGTLIRAQAAGDLGHAAPVRAQGAAWPRSPWTAPTRSSAWPRPWRT
                     ASRIPERTRMPAIRRTLTVFAVAGLAWLAIVLTRGALDPPAATLPVRPDSSPASSSPA
                     PAIRRPPLLAGEWQELEPAPIGVPGEHLGAWTGRELVVGDFATGMAAYDPRARRWRVL
                     RGAPRLQRVGAKLAAGEDGRVFLYGGFAPSYNPRPYTADAWMMDRAGRWRALPPPPAP
                     PRWVPAVVWTGSELFVWGGERPGDWMARDAATGWVGGSAYDVAAGRWRPLPPAPIADV
                     WYARGVWAGDRVLVWGQTAAHESFAAAYLPATDEWRPLPPPPLRDPTDAAAVWTGTEL
                     VLWGRPRTEAPAESAAPDGVALAPAAGAQWRPLPLVGLGGVPARDSYGYAAAWTPHGM
                     VLHGGYPRAVSLRYDPAAGSWARLPHGGPGPRVTPVLAWTGAEVLVWGGFGTSGPDGR
                     LWAWRPPRASPE"
BASE COUNT         1388 a         3544 c         3568 g         1337 t
ORIGIN      
        1 atcgtgggct gcacgggcgg cggctccaac ttcgcggggc tgagcttccc gttcctgcgc
       61 gagaagctgg ccggcaagat ggacccgcgc atcctggcgg tggagccggc ggcctgcccg
      121 tcgctcacgc gcggcgtcta cacctacgac ttcggcgaca ccgcgggcat gacgccgctg
      181 gtgaagatgc acaccctggg gcacgacttc gtgcccgagg cgatccacgc cggcggcctg
      241 cgctaccacg gcatgtcgcc gctgatctcc cacatctacg agctcggcct catcgaggcg
      301 gtcgccaaga accagagcga gtgcttcgcc gcgggcgtcc agttcgcgcg caccgagggc
      361 atcgtgccgg cgcccgagcc gacgcacgcc ctggccgcca ccgtcgagga ggcgctgcgc
      421 tgcaaggaga ccggcgagga gaaggtggtc ctcacggcgc tgtgcggcca cggccacttc
      481 gacatggcgg cctacgaccg ctacctggcg ggcgagctgg tggactacgc ctacccggag
      541 gagaaggtgg ccgacgcgct cacgcgcctg ccgcaggtgg acctgtaacg cgaagggggc
      601 cggtctttcg accggccccc tcggtgtgcc gttcggctca ggcctcgaag cagggcttgt
      661 cgaacgccat gccctcggcc gtggggccgg tgaagaagtc ggcgacgacg ttcacgcccg
      721 tgggctgcgt gtcgtccacc tgggacacca cgctctggcg gaacacggcg tcggccggct
      781 cgcctgcgta gttgccggcc tcctccggca gcatgccctc gtagtcgacc gtctccagcg
      841 tcttcacggc ctccagcagg ccctcacggg tgaggttgcc gtcctcgacc gccttctcca
      901 gcgcggcctt gagcgggtac tgccacaccc agccggccac gtagccgtcg ttcggcgtgg
      961 tgtccgggaa ggcctcgcgc agggcggcgt gcccggcgga ctcggtgccc cacgggcccc
     1021 acggcccggc ctggaagtac atcgccttca gcgccggggc ggccgcggtc tgcagcagcg
     1081 cggggttcca cgtcgggctg gagccgatca ccttgccggt gaagccctgg ctggccgcgc
     1141 cgcccacgat ggtcgcgagc tccgtcggtc cggtggtcac caccacgagg tcgggcttct
     1201 cctgcacgat cgccgcgacg gcaccggact ggttctcctg gcccggctgg gtcgggtggt
     1261 cgacgaactc ggcgccgatg cgctcggcgg ccagccgcgc accggcggcc gcgtcgtcgc
     1321 cgtagtcacc ggggtagtgg atcgccatga ccttggtcgg cgccgcctgc tcctcggcgt
     1381 accagtcgac ggcgttggcc atctccatgc agtagttggt gccggactcg atgatgacgt
     1441 cctcgaactc ccacgcagac gtccacgagg ccggtgcggc cacgatggag tcgtcgcgca
     1501 ggtcctggat gatcgccgcc gtctgcgggg agccgagcgt ctgcgccagg cccagcacgt
     1561 cgccgcggat ctcctcgaac acctgcttgt gggtctccgg gatgtacttg ttgtccttga
     1621 tgtgggtctc gacgtcgatg tcgtagccgc cgatgccccc gtcgttgttc acgcgcgccc
     1681 agaacgcctt ctgggcgtcc gtcagcggga cggcgagggc gcggaacggg ccctccgtca
     1741 ggtcggagat gatgcccagg tagatgcagc cgttgtcggc gttgaccgcg ttcggacacg
     1801 ggtcgtcggt gacaccgacg gcgctgccgc cgccgtcacc tccgtccccg tccgtgtccg
     1861 cgtcgtcgtc gccgcctccg caggcggtcg ccacgaggga gagcaccagc atcaagatgg
     1921 cgagcatccg ccagcgtgtc gttgccatgg gtgtttcctt tctgccgccc gatgtcgccg
     1981 ttcccgctag taggagaacg gccacccttt ccagtagttg cgcacgcgca gccacacgcc
     2041 gaacaggccg cgcggttcga acaccaggaa cccgatgatg agcaggccgt acagcacggc
     2101 ctcgatctga tgctggtcca gcagctgccc cccggacagg aacggcagct cgcgcacgag
     2161 gaactgcgac atgcgcggga gcatggtgat gaacaccgcg cccatgatcg ccccggacac
     2221 cgtggcgatg ccgccgatga ggatcatcgc gatgaagcgg atcgacatca gcaggttgaa
     2281 cgagctcggc tcgatgtgcc cgatgatcgt gtacagcagc gcgccggaca cgcccgcgaa
     2341 cgccgaggag atggtgaacg cgagcacctt gtacttggtc agcggcacgc cgatgaccgc
     2401 cgcggcgatg tcgcggtcgc gcacggcgga gaacgcgcgg ccgagcgcgg agcgcccgac
     2461 gttgcgcccg agcaccgcgc acacggccag caggaccagc atgagcaggt acagcttcac
     2521 gtgccgggtg agcgtcaggc ccagcagcgt gctgggctcg gtgaggtcga cgccgagcac
     2581 caccgcctcc gggccgggcc ggcccacgcc gaccccgccg gtgaggtcct tgagctcttt
     2641 gaaggcgtgc tcgcccagga acaccaggcc caaggtgacg atggccaggt acagcccgcg
     2701 cagccgcacg gccagggggc tgaccagcgc gccgaacgcc ccggccacca ccgccgcgcc
     2761 gggcagccac agcagcatgt ccaggccgaa gccgatgtgg cgggagctgt ccgggtcgcc
     2821 ggccagcgtg gcggcggtga acgcgccgac gcccacgaag aacgcgtggc ccagcgagac
     2881 ctggccggcc atgccggtga cgatgttcaa gccgatggcg ccgatcgcgg cggccgccgc
     2941 cgtggccagc agcagcacca tgtcgttgcc caccaggaac ggcagcacca cgccggccag
     3001 gaacagcgcg gtcacgcccg cgcgcttcgg cgccgtgttc agcagggcct ggtcctggcg
     3061 gtaggacgtg tacagctccg ggcggccgcg gacgcgccgg ccgcccagct cgcccagctc
     3121 gggcgcatgc gtcgtggcgc tcatacgcgc tccacctccg gcgtgccgaa caggccgtac
     3181 ggccgcacga gcaggaccag cagcatcacc aggtagggcg agaccacgga gaagttcgcg
     3241 cccagccagg ggaagtagct gccctggtag gtggcgacca gcgcctccac gacgccgatc
     3301 aggatgccgc cgacgacggc gcccggcacg gagtcaaggc cgccgaggat gatggcgggc
     3361 agcgccttca gcgccaccgt ccacgtggag ccttcgatgc cggtgccggt gccgacgaac
     3421 gcgccggcca cggcggcgag cgccccggcg atcgcccacg acagcgcgaa caccatgccc
     3481 accgacacgc cctgcgcctg cgcggtctcc tggtccagcg cggctgcgcg catcgccagc
     3541 cccacgcgcg agtacttgaa gaacagcgac agcgcggcca ccagcacggc cgtggtcagc
     3601 agcatggcga tgtggcgctg ctgcacgatc acgccgccga aggtggtggt cgacagcccc
     3661 cacgggttgt cgaccgggcg cacgtccagg ccgatgaagc ggttcaccac cacgcggatc
     3721 accacgtcga caccgatggt gatgatcgcc accacgaaca cgggcttgcc gaccatgggc
     3781 cgcagcgcgg tgcgctccac caccagggcg agcaccgcgg tggccgcggc ggccagcgcc
     3841 agcgccggga agaagcccag gcccacgacg tggccgaaca ggtaggtgag catcgcgccg
     3901 gcgaccatga tcgacggctg ggcgaagctg atcaccgacg tggccttgta gatgatcacg
     3961 aagcccaggg ccagcagcgc gtagatgctg ccgttgccca gcccgaccac gatggcctgc
     4021 gcgaggcggg tcactggtac atcgcctcca ccaggtcgct gaactggcgg gagatcgcgg
     4081 cccgcttcac cttctgcgtg gcggtcagct cgccgtcctc gtgatcgagc tccttgggca
     4141 gcatgcggaa cttcttgatc gtctccacct gcgcgaagcg cgcgttggtg cggtcgatct
     4201 ccttgcgcac cagggccagc acctccggct tctccgacag gtcccggtag gtggtgtagg
     4261 ggatgccctg gcgggtggcc cagtcgccca ccgtgtccag ctcgatgccg atcagcgccg
     4321 tgaggtaggg gcgccggtcg cccaggacca cggcctcctt gatgtagggc gaggtcttca
     4381 gcgcgttctc gagctccgac ggcgagatgt tcttgccgcc ggccgtgatg atgatgtcct
     4441 tcaggcggtc ggtgatcttc aggtgcgtgc cgtcgaccca ctcgccgacg tcgccggtgc
     4501 gcagccagcc gtcctcggtg aaggtctcgc gcgtggcctg ctcgttgcgc cagtagccgg
     4561 cgaagttgcc cgggtggcgt gtctggatct cgccggtggc ggggtcgacg cgcacctccg
     4621 ccccggggta gggctcgccg acggtgccga gcttgacgcg tccgggacgg ttggacgtcg
     4681 ccacggcgct gttctcggtc atgccgtacg cctcgtgcat cggcacgccg atggccatga
     4741 agaagcgcag caggtcgggc gcgaccggcg ccgccccgga cgcggcgaag cggcagcagc
     4801 gcatgccgat gcggtccttg agcgcccggt acagcagcag gtggccgagc agccgcacga
     4861 cgcggtccag cggggtcagg cgcgcaccgg ccaggtcctt gtccaccagc cgcagcccgc
     4921 gcgccaccca gaagcggtag ttggcgcgct tgagccggct ggcgttggcc atgcggatct
     4981 cgatgcccgc gtgcatcttc tcccagatgc gcggcacgcc caggaagatc gtcggctgga
     5041 tctcgcgcag gttctgctgc accgtctcga tggactcggc gaagttcacc accggcccgg
     5101 cgccgaggtt gaaccacacc gtgaacaggc gctcggccac gtgcgccagc ggcaggtacg
     5161 acagcagcat gtccgactgg cccggcgggg ggtcggccag cgcgccggag accacggccg
     5221 acgtgatggc gaactcgacg ttggcgacgg tcagcatcgc gcccttgggc gggcccgtgg
     5281 tgccggaggt gtagaccagc gtcgccaggt cgtccgggcg cgcctggctc atcaggcgct
     5341 cgacctcgcc ggcgtgcgcg gcacggtgcc ggcggcccat cgccagcagg tcctcccagg
     5401 ccagcaggcg cgggtcgtcg tagcggcgca ccccgcgggg gtccacgtag acgatgcgct
     5461 ccaggtgggg gaggcggtcg gcgaccgcca gggccttgtc gacctgctcc tggtcctcgg
     5521 ccacgaggat cttggccccg gagttgtcca gcaggtactc cacctcggcg gccgggttgg
     5581 tggggtacac gcccatgctc acggcgcgga ccgccacgca cgcgacgtcg cagaccaccc
     5641 attcgggccg gttctcggag tggatcgcga cacggtcgcc gggctccacg cccagggaca
     5701 ggaacgcgtg cgccacgtcc tggaccaggt cccagtaggc ctgccaggtg atctcgtgcc
     5761 acacgcccag ccgcttctcg cgcagggcga cgccgccggg gtcgcgatgc gcccagtcgc
     5821 gcagccgcgt ggcgatgctg atggtggtgg cgggtgcctc gaaggtcgtg gtcatgtcgt
     5881 gcccccttcg ccgggcgtcg cggcggccgc ccccaggtac gcgcggatca cgtcggggtg
     5941 gcgctggacc tcgtcgggcg tgccggtcgt gatcggcttg ccgaagtcga gcaccatgac
     6001 gcggtcggcc aggtccatga cgacgcccat gtcgtgctcg accaggatga tcgggatgcc
     6061 cagctcctcg cggatgtcca ggacgaagcg cgccatgtcc tcggtctcct cgacgttcat
     6121 gccggcgacc ggctcgtcga gcagcagcag cttgggctcc atggccagcg cccgtccgag
     6181 ctcgacgcgc ttctgcacgc cgtagggcag caggcgcacc gggtacttgc ggtgctcctc
     6241 gatctccagg aagtcgatga tctcctcgac cacggcgcgg ttgcggacct cctcgcgggc
     6301 ggcgcggccg gcgtaggcca gcgcggacac ggcgccgtag cgcacgtgcc cgtggcggcc
     6361 gagcatgagg ttgtcgacca cggtgaggtt ctcgaacagc tcgatgttct ggaaggtgcg
     6421 cgccatgccg agctgggcga tgcggtgcgg cgccatgccg acgagctcct ggccgtcgaa
     6481 gcgcaccgag ccctcctggg gggcgtagat gccggacagc acgttgaaca cggaggtctt
     6541 gccggcgccg ttggggccga tgatcgcgaa cagctcaccg ggttggacgg tgaagctcac
     6601 gccgtccagt gcggtgacgc cggcgaagcg caggcgcacg tcgcggaagt ccagcaagct
     6661 catgacagcc accgcttgcg ccgcttgtag tgcttgacgt cgcggaagga cttcgccgcg
     6721 ccctccgtgc ccatgcccag gtagaactcg cgcacgtcct cgtcctccag cagggcggcc
     6781 gcggcgttgt ccatgacgac cttgccggtc tccatgacgt agccgtggtc ggcgatggac
     6841 agcgccatgt gcgcgttctg ctcgaccagc agcaccgtgg tgccctgctt gttgatctcc
     6901 accaccaggt cgcggatctg ctccacgagc agcggcgcca ggcccaggct cggctcgtcc
     6961 agcagcaggt agcgcgggtc ggacatgagc gcgcggccca tcgccagcat ctgctgctcg
     7021 ccgccggaca ggtagccggc ggtcgagcgc gcgcggtcct tgagcaccgg gaacagctcg
     7081 tagacgcggt ccaggttgtg cttggccgtc ttggcgttgg tgtagccgcc gatgcgcagg
     7141 ttctcctcga cggtgaactc cgagaagatg cgccggccct ccagcacctg cgtgacgccg
     7201 gcgcgcacga tgcgcgcggg cggcaggtgg tggatcgggc tgccgtccag cgagatgcgg
     7261 cccttggtga tctcgccgtc gtgcacgtcc agcagcccgc tgatggcgcg caggagcgtg
     7321 gtcttgccgg cgccgttggc gcccagcagc gccacgatct tgccgtcggg gacctcgagg
     7381 ctcacgcctc gcagcacgag gatgacgtcg tcgtagacga cctcgaggtt ctccacggtc
     7441 aggaccaacg gcgcgctccg gtcagcaggc tgtggactga tggacggcta ctgggcggtg
     7501 cctggcggct acggctgcga tcgtagagaa ctcggcagcg gaaatcctgg gttttccgcg
     7561 tgccataccc aagttggtca ttccgggctg gcccgcggcg gtcgccaggc ccagagccgg
     7621 ccatccgggc cgctcgtgcc gaagccaccc cacacgagca cctcggcgcc cgtccacgcc
     7681 agcaccggcg tgacccgcgg gccggggccg ccgtgcggca ggcgcgccca ggacccggcc
     7741 gccgggtcgt agcgcagtga caccgcgcgc gggtagccgc cgtgcagcac catgccgtgc
     7801 ggggtccacg cggccgcgta cccgtaggag tcccgcgccg ggacgccgcc cagcccgacc
     7861 agcggcaggg gccgccattg cgcgccggct gccggcgcca gggccacgcc gtcgggagcg
     7921 gcggactcgg cgggcgcctc ggtgcggggg cggccccaca gcaccagctc cgtcccggtc
     7981 cagacggccg ccgcgtctgt cgggtcccgc agcggcggtg gcggcagggg ccgccactcg
     8041 tcggtcgcgg gcaggtacgc cgcggcgaag gactcgtgcg cggccgtctg gccccaaacc
     8101 agcacgcggt cgcccgccca gaccccgcgc gcgtaccaca cgtcggcaat gggcgcgggc
     8161 ggcagcgggc gccagcggcc ggcggccacg tcgtacgcgg acccgccgac ccagccggtg
     8221 gcggcgtcgc gggccatcca gtcgcccgga cgctcgccgc cccagacgaa cagctcgctg
     8281 ccggtccaca ccaccgcggg cacccatcgc ggcggcgccg gcggaggcgg cagggcacgc
     8341 cagcgcccgg cccggtccat catccacgcg tcggcggtgt acgggcgcgg gttgtagctc
     8401 ggcgcgaagc ccccgtacag gaacacgcgg ccgtcctcgc ccgcggccag cttcgcgccg
     8461 acgcgctgca ggcgcggcgc gccccgcagg acccgccagc ggcgggcgcg ggggtcgtag
     8521 gcggccatgc cggtggcgaa gtcgcccacg acgagctcac ggccggtcca cgcgcccagg
     8581 tgctcgcccg ggacgccgat gggcgcgggc tccagctcct gccattcgcc ggcgaggaga
     8641 ggcggccggc ggatggccgg cgcgggggag gacgaggcgg gggagctgtc cggccgcacc
     8701 ggcagtgtcg cggccggagg atcgagcgcg ccgcgcgtga gcacgatcgc gagccacgcc
     8761 agccccgcca ccgcgaacac ggtcagcgtg cggcggatgg cgggcatgcg cgtgcgctca
     8821 ggaattcgtg aggccgtgcg ccagggcctc ggccaggcgc tcgaccgcgt cggggccgtc
     8881 cacggcgacc tgggccacgc ggcgccctgc gcgcgcacgg gcgccgcgtg gcccaggtcg
     8941 ccggccgcct gcgcgcggat cagcgtgccg aagtcgtagg gccgcccggg gatgtcgacg
     9001 ggcgccgcgt cctgccacag cgtggcgtcg acgatctgga gcaccacgac gttgtccgcg
     9061 ccgcccttgt gcagctgccc ggtggagtgc aggaagcgcg gcccccagcc gaacgtggtg
     9121 gcgatcccgc gcaggtcgcg ggcggcggca cgcgcctggg cgaacgacgc cagcgtcgcc
     9181 gacgagggcg gcaggtacgc ctggatcgcc aggtagtcgc ccgggcgcag agtggcgcac
     9241 agcgccggca cgtcgccgtg ctcgggcgcg ggcacgccgc cgccgccggc gacttcggcc
     9301 aggatcgccc tggtctgctc cttggcctcg gtgacgttgg gctcgtcgaa gggattcacg
     9361 cccagcagcg cgcccgccag ggcggtggcc agctcccagc gcaggaactc cgcgccgagg
     9421 tgctcgcgcg acgccacgtc caggaacagc acggggtggc cggcgtcggc cagcgcacgg
     9481 gcgccctcgg gctcggtgtc gccgaagcgg taggcgacga aggcgcggtc gtcgccgtag
     9541 gagccgggcc ggccgacggg ctcgccgacg acgggcacca cgcccacgcc ctgtttgccg
     9601 gtgctctcgg cgacgagctg ctcgacccag tcgcccagcg ggccgagcgg ctccggcgcc
     9661 agcagcgtga gcttgtccct gccgccgcgc gcgagtcccg ccatgaacgc ggcgagctgc
     9721 acgcccgggt tcgccgcggc cgggacaccc ggtccggccg cgaccagcag ctcggcggcg
     9781 tggccccaga cggcgtcgac gtccacgccg agcagcgcgg cgggcgccat gccgaac
//
LOCUS       DATMKC010000391         3277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_168171, whole genome shotgun sequence.
ACCESSION   DATMKC010000391 DATMKC010000000
VERSION     DATMKC010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168171"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..988
                     /locus_tag="VNU01_11415"
     CDS             152..988
                     /locus_tag="VNU01_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH33267.1"
                     /translation="MRFDNPELVDTSAGRVAVHQAGQGSPVVLLHGLFGASPVWSGVA
                     PLLAARHRVLAVDLLGFGHSERVRDAEALWAPSQAAAIGEALDALGVGPAAFVGYDYG
                     GPVALTLSARAPQRVTALTLSATNAFPDAPMPFPLSLLFLPVVGGAAEAALFSRPGLA
                     MAARAAVGTKGVRLDRWLLLGDRAQSRAVRTLFKQALRQMPARYGPVHDALTRLDVPT
                     LVVWGDRDPFFALEQGRRTAAAVRGAQLRVYDGAGHFVPEEQPERFAADVLALLARVG
                     AA"
     gene            1013..3025
                     /locus_tag="VNU01_11420"
     CDS             1013..3025
                     /locus_tag="VNU01_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWH33268.1"
                     /translation="MFDSVLVANRGEIAVRVIRTLRRLGIRAIAVHSDADAGAPHVRA
                     ADLAVRLGPTPAAESYLHTERVLEAAGATGAQAIHPGYGFLSENAAFARACAAAGIVF
                     VGPPPEAIEAMGDKIRAKQTVEPAGVPVVPGVHRPGLDDDALTRAAEGVGYPLLVKAA
                     AGGGGKGMRIVRAAGELAEALSAARREARNAFGDDDLLLERYLERPRHIEIQVLADTY
                     GNVVHLGERECSLQRRHQKVIEEAPSPVLDAAMRARMGAAAVGAATSCGYTGAGTVEF
                     IVPFTPAGQTPSEFFFLEMNTRLQVEHPVTELVTGLDLVEQQLRVAAGQPLGFTQDDV
                     TLTGHAVEARVYAEDPARGFLPTGGRILVYAEPSGDGVRVDSGVAAGVEVGSAYDPML
                     AKVIAHGPDRATALGRLDAALRDFTLLGPTTNTAFLRVLLDHPDVAAGALDTSLAERV
                     ADDLPPAGLPHDVLAAAALARLLALEPDGPVVDPFTIPGGWRIGEAAWAVWLLRQEGG
                     DADPVEVRVRGRAARAEVAVADEAPLVASARWEGPHRLLVTAAGQTRAYAYAERSGTA
                     LLGRDGATWAIREEDRLEAARHHDAAAGVGPLLAPMPGTVTVVHVEPGETVAAGQTLL
                     VVEAMKMEHPIKAPADGVVAELAVAAGDRVDMDQRLAVVAPDGGEA"
     gene            3022..>3277
                     /locus_tag="VNU01_11425"
     CDS             3022..>3277
                     /locus_tag="VNU01_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33269.1"
                     /translation="MSGRRAHALDGLPERITIYEVGPRDGLQNEAATVPTEVKREFIE
                     RLADAGLSVVEATSFVRPEWVPQLGDAEALMTTLERRDGVR"
BASE COUNT          376 a         1225 c         1267 g          409 t
ORIGIN      
        1 gtgtccagcg cccgcccacc acgtcgagcg ccttgctcag cgcgcagagc tgctcgtagg
       61 agcgcatgcc tgacctttcg gttgagtttc ttgctcggct agtttacttt cttgccctag
      121 gagagcaagt gggcgagcaa ggaggcgggc gatgcggttc gacaacccgg aactggtgga
      181 cacctcggcg gggcgcgttg ccgtgcacca ggccggccag ggcagccccg tggtgctgct
      241 gcacggtctc ttcggcgcct cgccggtgtg gtccggcgtg gcgccgctgc tggcggcgcg
      301 gcaccgcgtc ctggcggtgg acctgctcgg cttcggccac agcgagcgcg tccgcgacgc
      361 cgaggcgctg tgggcgccca gccaggccgc cgcgatcggc gaggcgctgg acgccctggg
      421 tgtcggcccc gcggcgttcg tgggctacga ctacggcggc ccggtcgccc tcacgctgag
      481 cgcccgcgcg ccgcagcgcg tcacggcgct gacgctgagc gccacgaacg ccttcccgga
      541 cgcgccgatg ccgttcccgt tgtcgctgct gttcctgccg gtggtgggcg gggcggcgga
      601 ggcggcgctg ttctcccggc cgggcctggc catggccgcg cgcgcggcgg tgggcaccaa
      661 gggcgtgcgc ctggaccgct ggctcctgct gggcgaccgg gcgcagtccc gcgccgtccg
      721 cacgctgttc aaacaggccc tgcgccagat gcccgcccgc tacggcccgg tccacgacgc
      781 gctgacacgg ctcgacgtgc ccacgctggt ggtctggggc gaccgcgacc cgttcttcgc
      841 gctggagcag ggcaggcgca cggccgccgc ggtgcgcggc gcgcaactgc gcgtgtacga
      901 cggcgccggg cacttcgtgc cggaggagca gcccgagcgc ttcgccgccg acgtgctcgc
      961 gctgctggcc cgggtggggg cggcctgacc gcggcgcata ggattcgcgg cgatgttcga
     1021 ctccgtgctc gtcgccaacc gcggggagat cgccgtccgg gtgatccgca ccctgcgccg
     1081 cctcggcatc cgcgccatcg ccgtccacag tgacgcggac gccggtgcgc cccatgtccg
     1141 ggccgcggac ctcgccgtgc gcctcgggcc gacgcccgcc gccgagagct acctgcacac
     1201 cgagcgcgtg ctggaggcgg ccggcgcgac cggggcgcag gcgatccacc ccggctacgg
     1261 gttcctgtcg gagaacgccg cgttcgcgcg cgcctgcgcc gcagccggca tcgtcttcgt
     1321 cggcccgccg ccggaggcga tcgaggcgat gggcgacaag atccgcgcca agcagaccgt
     1381 ggagcccgca ggggtgccgg tggtgcccgg cgtgcaccgg ccgggactcg acgacgacgc
     1441 gctgacgcgg gcggccgagg gcgtcgggta ccccctgctg gtcaaggccg ccgcgggcgg
     1501 cggcggcaag ggcatgcgca tcgtgcgcgc cgccggcgag ctggccgagg cgctgtccgc
     1561 ggcgcggcgc gaggcgcgca acgccttcgg cgacgacgac ctgttgctgg agcgctacct
     1621 ggagcgtccc cgccacatcg agatccaggt gctcgccgac acctacggca acgtggtgca
     1681 cctgggggag cgcgagtgca gcctgcagcg ccgccaccag aaggtgatcg aggaggcgcc
     1741 ctcgcccgtg ctggacgccg cgatgcgcgc ccgcatgggc gccgccgccg tcggcgccgc
     1801 gacgtcgtgc ggctacaccg gcgcgggcac cgtggagttc atcgtcccgt tcaccccggc
     1861 gggccagacc cccagcgagt tcttcttcct ggagatgaac acccgcctgc aggtcgagca
     1921 cccggtcacc gagctggtca ccggcctgga cctggtggag cagcagcttc gcgtcgcggc
     1981 cggtcagccg ctcggcttca cgcaggacga cgtgaccctg accggccacg ccgtggaggc
     2041 gcgcgtctac gccgaggacc ccgcgcgcgg cttcctgccc acgggcgggc gcatcctggt
     2101 ctacgccgag ccgtccgggg acggcgtgcg cgtggactca ggcgtcgccg ccggcgtcga
     2161 ggtcggcagc gcctacgacc cgatgctcgc caaggtcatc gcgcacgggc cggaccgggc
     2221 cacggcgctg ggccgcctgg acgccgcgct gcgcgacttc acgctgctgg gccccaccac
     2281 gaacacggcg ttcctgcgcg tgctgctgga ccaccccgac gtggcggccg gggcgctgga
     2341 caccagcctg gccgagcgcg tcgccgacga cctgccgccc gcgggcctgc cgcacgacgt
     2401 gctcgccgcc gccgccctgg cgcgcctgct ggcgctggag cccgacgggc cggtggtcga
     2461 cccgttcacc atccccggcg gctggcgcat cggcgaggcg gcgtgggccg tgtggctgct
     2521 gcgccaggag ggcggcgacg ccgacccggt ggaggtgcgc gtgcgcggcc gcgccgcccg
     2581 cgcggaggtg gccgtcgccg acgaggcgcc gctggtggcc tcggcgcgct gggaggggcc
     2641 ccaccgcctg ctggtcaccg cggcgggcca gacgcgcgcg tacgcctacg ccgagcgctc
     2701 tggcactgcg ctgctgggcc gggacggcgc gacgtgggcg atccgcgagg aggaccgcct
     2761 ggaggcggcg cgccaccacg acgccgccgc cggcgtgggc ccgctgctcg cgccgatgcc
     2821 gggcaccgtg accgtggtcc acgtcgagcc cggcgagacc gtggccgccg ggcagacgct
     2881 gctggtggtc gaggccatga agatggagca ccccatcaag gcgcccgccg acggcgtggt
     2941 ggccgagctg gcggtggcgg ccggagaccg cgtcgacatg gaccagcgcc tggccgtggt
     3001 cgccccggac ggcggcgagg cgtgagcggc cgccgcgcgc acgcgctgga cggcctgccg
     3061 gagcgcatca cgatctacga ggtcggcccg cgcgacggcc tgcagaacga ggccgccacc
     3121 gtgcccaccg aggtgaagcg cgagttcatc gagcggctgg ccgacgcggg gctgtccgtg
     3181 gtcgaggcca cgagcttcgt gcggcccgag tgggtgccgc aactcggcga cgccgaggcg
     3241 ctgatgacca cgctggagcg ccgcgacggc gtgcgct
//
LOCUS       DATMKC010000392         3691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_351654, whole genome shotgun sequence.
ACCESSION   DATMKC010000392 DATMKC010000000
VERSION     DATMKC010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351654"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VNU01_11430"
     CDS             <1..679
                     /locus_tag="VNU01_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWH33270.1"
                     /translation="IEHATLLSPLPEPGELRAHARPGPAEGTVVLSVVAADRLGLVAD
                     CAGVLAGHGLTVLDARAFTRADGVALDWFVLRPGKGHGQASWEQVTDDLRRAAQGRFD
                     VAGHVARRERRRDVRPRVAPAMEEVEVRLERLDDGSLRIEVHGPDAPGVLYRLARVLA
                     DQGLDLAGARVGTLGPEVRDVFFVRAPDGDLDQAMLAALLTDAGGWPADVLPQPPARR
                     RRASPAA"
     gene            692..1168
                     /locus_tag="VNU01_11435"
     CDS             692..1168
                     /locus_tag="VNU01_11435"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HWH33271.1"
                     /translation="MDVHPQIQPLAFLLGTWRGEGQGRYPTIADFAYGEEIAFAHVGK
                     PFLAYTQRTWALDDQRPLHAETGYLRRTAEGALEWLLAHPTGIAEVELGSLDGATLRL
                     RSVGVLCTPTAKRVDELTRELTVDGDELRYELHMAAVGEPLTFHLSATLRRVRDRG"
     gene            complement(1172..1900)
                     /locus_tag="VNU01_11440"
     CDS             complement(1172..1900)
                     /locus_tag="VNU01_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWH33272.1"
                     /translation="MTGVLVHLVVYAAVNGLLLGIWYLAGAATPFWPVWVLAPWGAGL
                     AIHAGVVAVTAPARMRRRRRRRRAAAASAGRTWIAAMFTDLVGSSALAERLGDEAWAR
                     LIGSYRRTVETVAAAHGGAVIGTQGDGVLLRFDSPAKALRAAAALQRQFARRRDDGDA
                     LPPVRVGIHAGEAVAVGGDVLGQMVNVAARIADAAGADEVLVSEPVADHAPPEMAFDD
                     RGLQSVKGSDKARHILALRWRGVE"
     gene            complement(1932..2630)
                     /locus_tag="VNU01_11445"
     CDS             complement(1932..2630)
                     /locus_tag="VNU01_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33273.1"
                     /translation="MSRRAACLLILALLASACGGGGGDDAGGSGAGELRGAFAVVAGA
                     CAPDGTRSGSFFRMVQPGGTLEEGPFVANADSPCDDKTWTPLRPGTDGGLVTGDYQPH
                     PEPPFAANDGAAAAIVEPTSFFAVRFAAATNPTDPQTGQAVPAPSVSVDEQGALHGDL
                     SAFGVAWNQQHFNQGAPKPGGARPGLTAGPSGTYDEDTGAYALEWSSQIEGGPFNNFT
                     GVWHLEGTFAPAAE"
     gene            complement(2627..3520)
                     /locus_tag="VNU01_11450"
     CDS             complement(2627..3520)
                     /locus_tag="VNU01_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33274.1"
                     /translation="MRSRGPLLVILAVGVGLAAAPMVFQMFTRAPLGGQMIEEFRPFM
                     SPARLDAFEGHLTRIGAAVESLHRRGAGLEHPLVEPLATQWPEIDADMTGMLDDIRDN
                     LDNFAAVAALPPFPLFPWFFLAPGLLLAWGAGAGLRRADHDGLPASTRAVLVALGLGL
                     VAAPAVFQMFERAPLGGDMIADFRSLMTEERIQSVQGYFLVIGAAEGALRTEVLEELE
                     ADGMRAVDVRNLVDAWPAMAADMAPMIGAMSDNLDNYRAVAALPPFGLFPWFFVIPGL
                     LVAGLALLPPGTATAQKETTP"
BASE COUNT          448 a         1411 c         1404 g          428 t
ORIGIN      
        1 catcgagcac gccacgctac tgtcgccgct gcccgaaccg ggcgagctgc gcgcccacgc
       61 ccgcccgggc ccggccgagg gcacggtggt gctcagcgtc gtggccgccg accggctcgg
      121 cctggtcgcc gactgcgccg gcgtgctggc cggccacggg ctgaccgtgc tggacgcgcg
      181 ggcgttcacg cgcgccgacg gcgtggccct ggactggttc gtgctgcggc ccggcaaggg
      241 gcacgggcag gcgtcgtggg agcaggtgac cgacgacctg cgccgggccg cgcagggacg
      301 cttcgacgtg gccggccacg tcgcgcgccg cgagcgccgc cgggacgtgc ggccgcgcgt
      361 ggcgccggcg atggaggagg tggaggtgcg cctggagcgc ctcgacgacg gctcgctgcg
      421 catcgaggtg cacggtccgg acgcccccgg cgtgctgtac cggctggcgc gcgtgctggc
      481 cgaccagggc ctggacctgg ccggtgcgcg cgtgggcacg ctggggccgg aggtgcgcga
      541 cgtgttcttc gtgcgggcac ccgacggcga cctcgaccag gcgatgctgg cggcgctgct
      601 caccgacgcg ggcggctggc cggccgacgt gctgccgcag cccccggccc gccgccgccg
      661 cgcctcaccg gcggcctaga ctgcgcgcct catggacgtg catccccaga tccagccgct
      721 cgcgttcctg ctcgggacgt ggcgcggcga gggacagggc cgctacccga ccatcgcgga
      781 cttcgcctac ggcgaggaga tcgcgttcgc gcacgtcggc aagccgttcc tggcctacac
      841 ccagcgcacg tgggcgctgg acgaccagcg ccccctgcat gccgagaccg gctacctgcg
      901 gcgcacggcc gagggcgcgc tggagtggct gctggcgcac cccaccggca tcgccgaggt
      961 cgagctgggc agcctcgacg gcgcgacgct gcggctgcgc agcgtcggcg tgctgtgcac
     1021 gccgaccgcc aagcgggtcg acgagctcac ccgcgagctg acggtggacg gcgacgagct
     1081 gcgctacgag ctgcacatgg ccgccgtggg cgagccgctg accttccacc tgtcggccac
     1141 gctgcggcgc gtgcgcgaca ggggctgatc gtcactccac gccgcgccag cgcagcgcca
     1201 ggatgtgccg ggccttgtcg ctgcccttca ccgattgcag gccgcggtcg tcgaaggcca
     1261 tctcgggcgg cgcgtggtcg gccaccggct cgctcaccag gacctcgtcg gccccggccg
     1321 cgtcggcgat gcgcgcggcg acgttgacca tctgccccag cacgtcgccg cccaccgcga
     1381 ccgcctcgcc cgcgtggatg ccgacgcgca ccggcggcag cgcgtcgccg tcgtcgcgcc
     1441 ggcgggcgaa ctggcgctgc agggccgccg cggcccgcag cgccttggcg ggggagtcga
     1501 agcgcagcag cacaccgtcg ccctgcgtgc cgatcaccgc gccgccgtgc gcggccgcca
     1561 ccgtctcgac ggtgcgccga tacgagccga tcagccgcgc ccaggcctcg tcgcccaggc
     1621 gctcggccag cgcgctggag ccgaccaggt ccgtgaacat cgccgcgatc caggtgcgcc
     1681 ccgcgctcgc ggccgcggcg cggcgccggc gccggcggcg gcgcatgcgc gcgggcgcgg
     1741 tgaccgccac gacgccggcg tggatcgcca gccccgcgcc ccacggcgcc aggacccaca
     1801 ccggccagaa cggcgtggcg gcgccggcca ggtaccagat gcccagcagc agcccgttga
     1861 cggccgcgta gaccaccagg tggacgagca cgccggtcac gcgcgccgcc tcggtgggcg
     1921 ccgccccccg ctcattcggc ggcgggggcg aacgtgccct ccaggtgcca cacgccggtg
     1981 aagttgttga acggcccgcc ctcgatctgg ctggaccact ccagcgcgta ggcgccggtg
     2041 tcctcgtcgt aggtgccgga cggtcccgcg gtgagtcccg gccgggcgcc gccgggcttg
     2101 ggggcgccct ggttgaagtg ctgctggttc cacgcgacgc cgaacgccga caggtcgccg
     2161 tgcagcgctc cctgctcgtc cacggacacg ctcggcgcgg gaaccgcctg gccggtctgc
     2221 gggtcggtcg ggttggtggc cgcggcgaag cgcacggcga agaacgacgt gggctccacg
     2281 atcgcggctg ccgcgccgtc gttggccgcg aacggcggct cggggtgcgg ctggtagtcc
     2341 cccgtcacca ggccgccgtc cgtgcccggg cgcagcggcg tccaggtctt gtcgtcgcac
     2401 ggggagtcgg cgttggccac gaacgggccc tcctccaagg tgccgcccgg ctgcaccatg
     2461 cggaagaacg agccggagcg ggtgccgtcg ggcgcgcagg cgccggccac gacggcgaac
     2521 gcgccgcgca gctcgccggc cccggagccg cccgcgtcgt cgccgccgcc tcctccgcac
     2581 gcggaggcca gcagcgccag gatgagcagg cacgcggcac gtcggctcat ggggttgtct
     2641 ccttctgcgc tgtcgcggtc cccggcggca gcagggccag gccggcgacg agcaggccgg
     2701 ggatcacgaa gaaccacggg aacaggccga acggcggcag cgccgcgacg gcgcggtagt
     2761 tgtccaggtt gtcgctcatg gcgccgatca tcggggccat gtcggccgcc atcgccggcc
     2821 acgcgtccac gaggttgcgc acgtccaccg cgcgcatgcc gtcggcctcg agctcctcca
     2881 gcacctcggt gcgcagggcg ccttccgcgg cgccgatcac caggaagtag ccctgcaccg
     2941 actggatgcg ctcctcggtc atcagggagc ggaagtcggc gatcatgtcg ccgcccaggg
     3001 gcgcgcgctc gaacatctgg aacaccgcgg gcgcggccac cagccccagc cccagggcga
     3061 ccaggacggc gcgcgtgctc gccggcaggc cgtcgtggtc cgcgcggcgc aggccggcgc
     3121 cggcgcccca cgccagcagc agcccgggtg ccaggaagaa ccacgggaac agcgggaacg
     3181 gcggcagcgc cgccaccgcg gcgaagttgt cgaggttgtc gcggatgtcg tcgagcatgc
     3241 cggtcatgtc cgcgtcgatc tccggccact gggtcgccag cggctccacc agcgggtgct
     3301 ccaggccggc gccgcggcgg tgcaggctct ccacggccgc gccgatgcgc gtgaggtggc
     3361 cttcgaaggc gtccaggcgc gccggcgaca tgaacgggcg gaactcctcg atcatctggc
     3421 cgcccagcgg ggccctggtg aacatctgga acaccatcgg cgcggccgcc aggccgacgc
     3481 cgaccgccag gatcaccagc agcggcccgc ggctacgcat ggcgccgcag ggcggcccgg
     3541 gccgcgaccg ccgcggcgag gaccgccagg ccggcggcgg tcgcggcccg ggccgccctg
     3601 cggcgccatg cgtagccgcg ggccgctgct ggtgatcctg gcggtcggcg tcggcctggc
     3661 ggccgcgccg atggtgttcc agatgttcac c
//
LOCUS       DATMKC010000393         3898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_227611, whole genome shotgun sequence.
ACCESSION   DATMKC010000393 DATMKC010000000
VERSION     DATMKC010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227611"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="fliI"
                     /locus_tag="VNU01_11455"
     CDS             <1..368
                     /gene="fliI"
                     /locus_tag="VNU01_11455"
                     /inference="COORDINATES: protein motif:HMM:NF037775.4"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HWH33275.1"
                     /translation="PVADAVRSILDGHIVLDRRLGISGRYPAVDPLASLSRLATKVTT
                     AEQQQALMRVREAMAAAEDVRDLVEVGAYVPGANPMADAGLALMPEIVRFLKQDTHEQ
                     TAYDEAWSRLAELAGRVVA"
     gene            365..784
                     /locus_tag="VNU01_11460"
     CDS             365..784
                     /locus_tag="VNU01_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33276.1"
                     /translation="MNKSRLAAVLRLRSLAERKARGQLAHAEREAALAAEMLDRRREA
                     PPPAVPSEVLTPLQLRALSLQGVRSHELLQAAAAEAQRTQKTRDQAHDHWAHASKEHK
                     SAERLDERRRSQIAEQARVAAERALDEMVVLRRGWAQ"
     gene            796..1344
                     /locus_tag="VNU01_11465"
     CDS             796..1344
                     /locus_tag="VNU01_11465"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWH33277.1"
                     /translation="MTAVQSRIAEIQSRFPVPRGDFRAALAHAERVQPPRNAATALPT
                     ANAEAGEWAAALPAAGQIEAAAHQAGVDPRLLASLVWAESGFRADARSHAGAIGLAQL
                     MPGTAAGLGVDARDPAQNLLGGARYLRAQLDRFGSPELALAAYNAGPARVAQAGGVPR
                     IAETQAYVPRVLGYFAQLGGVR"
     gene            1341..2555
                     /locus_tag="VNU01_11470"
     CDS             1341..2555
                     /locus_tag="VNU01_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33278.1"
                     /translation="MIDAVLAATLTAAPAGVQPNAPAEDSPAAGFGDVLAAMLAAAGE
                     VAPEAVVAAAAPAGPAGGSQEEQAAPDGRVAAAAPAAGEAPAILVGAAATEPAVRPAR
                     SEPRDAPPPAPQQSQGATAAKAASSAWHASAQGSAHAADASAVALSVPPVPPEPAPEP
                     VPAPVPEAAAEAGEAAPAETPAGEVAPATARETARGGEAGSAAAGAPARSDALAASAA
                     AGRAPSDDGGVQPLTGDGTARRAEPVARAEAAPRPASLTAAPGSEVAAPDATAPAPAA
                     APATAVPSGTLQRVLDAIEAASHRPPPSTMAVDLPADAGEELVRLLVTVRGDTVTVTG
                     GPSDQGLPPGWARPLAEALAQRGMALDGFASGQTPDRRRRGEPEPAPRGRPPAGQAVR
                     PSPRVHDGALRL"
     gene            2566..3006
                     /locus_tag="VNU01_11475"
     CDS             2566..3006
                     /locus_tag="VNU01_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HWH33279.1"
                     /translation="MPIDGISGIGGTAEPPKSSAVQSLGGMDSDAFLKLLVAQMRYQN
                     PMAPTDGAAMLQQTAQFTQVETLKQLATAQKELLSYHQASVASSLVGKEVTAVKDDGT
                     EVTGTVDAIRFTSDGPVLRMGELELSLDATREIRLAGAGGATSE"
     gene            3031..>3898
                     /locus_tag="VNU01_11480"
     CDS             3031..>3898
                     /locus_tag="VNU01_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWH33280.1"
                     /translation="MLRSMFAAVSGLRSHQTMMDVVGNNVANVNTAGFKSSRTTFQDA
                     LNQVIRGSSAVSDDRAGTNPMQIGLGSQVTAVDQVFGQGSIQLTGRNTDLAIQGEGFF
                     MMRIGEQTFYSRAGAFGLDANGSLTGPGGARVTGWMPDANGAIDTQQPLETLQLPTGQ
                     VIQPQESNNVTVGGNLPASAANGTAVNTSITIHDQQGTSYSLVLTFTKVGPDEWDMTQ
                     SLDGVAFGAAQDVFFDPATGEPLNAAAPGGTALAALTVDPAGTAGVWDGNGIEINFDE
                     VGVELRQFAGATT"
BASE COUNT          524 a         1492 c         1444 g          438 t
ORIGIN      
        1 agccggtcgc cgacgccgtg cgctccatcc tcgacgggca catcgtgctg gaccggcgtc
       61 tcggcatctc cgggcgctac ccggccgtgg acccgctcgc ctccttgtcg cgcctggcca
      121 ccaaggtgac cacggcggag cagcagcagg cgctcatgcg cgtgcgcgag gcgatggccg
      181 ccgcggagga cgtgcgcgac ctggtcgagg tcggtgccta cgtgcccggc gcgaacccca
      241 tggcggacgc aggcctcgcc ctgatgccgg agatcgtgcg cttcctcaag caggacaccc
      301 acgagcagac ggcctacgac gaggcgtgga gccgcctcgc ggagctggcg gggcgggtgg
      361 tggcatgaac aagagccgtc tggccgcggt gctgcgcctg cgttccctcg ccgagcggaa
      421 agccaggggt cagctcgcgc acgcggagcg cgaggccgcc ctggcggccg agatgctcga
      481 ccgacgtcgc gaggcgcccc cgccggccgt gccgtcggag gtgctgacgc ccctgcaact
      541 gcgcgccttg tcgctgcagg gcgtgcgctc gcacgagctg ctgcaggcgg ccgcggccga
      601 ggcgcagcgc acacagaaga cacgcgatca ggcgcacgac cactgggcgc acgcgtccaa
      661 ggagcacaag agcgccgaac ggctcgacga gcgccgccgc tcgcagatcg ccgagcaggc
      721 gcgcgtcgcc gccgagcgtg ccctggacga gatggtcgtc cttcgccggg ggtgggccca
      781 gtgagcgtcc atggcgtgac ggccgtccag tcccgcatcg cggagatcca gtcccgcttc
      841 ccggtgccgc gcggcgactt ccgcgccgcg ctggcccacg ccgaacgggt gcagccgccg
      901 cggaatgccg cgaccgctct ccccaccgcg aacgcggaag cgggggagtg ggccgctgcg
      961 ctgccggcgg ccggccagat cgaggccgcg gcgcaccagg ccggggtcga cccccggctg
     1021 ctcgcctcgc tggtctgggc ggagtccggc ttccgggccg acgcgcgcag ccacgcgggc
     1081 gccattggcc tggcccagct catgccgggc accgccgccg ggctgggcgt ggacgcgcgc
     1141 gacccggcgc agaacctgct cggcggtgcg cgctacctgc gcgcgcagct ggaccgcttc
     1201 ggcagcccgg agctggccct ggccgcgtac aacgccgggc ccgctcgtgt cgcgcaagcc
     1261 ggcggggtgc cgcgcatcgc cgagacgcag gcatacgtgc ccagggtcct gggctatttc
     1321 gcgcaactcg gaggggtccg atgatcgacg ccgtgctcgc cgccacgctg actgccgcac
     1381 cggccggcgt ccagccgaac gctcccgccg aggactcgcc ggccgcgggc ttcggtgacg
     1441 tgctggccgc gatgctggcc gccgccgggg aggtcgcgcc ggaggcggtc gtcgccgcgg
     1501 cggcccccgc ggggcccgcc ggcggcagcc aggaggagca agcggcgccg gacggtcgcg
     1561 tcgcggccgc cgcgccggcg gcgggcgagg caccggccat cctcgtggga gccgccgcga
     1621 ccgagccggc ggtgcggccg gctcgcagtg agccgcgcga tgcgccgccg ccggcgccgc
     1681 agcagtctca gggcgcgacg gcggccaagg cggcgtcgtc cgcctggcac gcctccgcgc
     1741 agggctccgc gcacgccgcc gatgccagcg ccgtggcgct gtccgtgccg cccgtgccgc
     1801 ccgagccggc gcccgagccc gtgccggcgc ccgtgccgga ggccgcggcg gaagctgggg
     1861 aggccgcacc ggcggagacg cccgccggtg aggtcgcgcc ggcgacggcg cgcgagacgg
     1921 cgcgcggcgg ggaagccggc tcggcggctg ccggcgctcc ggcacggagc gacgcgctcg
     1981 ccgcgtccgc cgccgcgggc cgggcaccct ccgacgatgg cggcgtgcag cccctgaccg
     2041 gtgacgggac ggcccggcgg gcggagccgg tcgcgcgtgc cgaggccgcc ccgcggccgg
     2101 catccctgac cgccgcgccc ggttctgagg tggcggcccc cgacgcgacg gcgcccgccc
     2161 cggccgccgc gcccgccacc gcggtgccgt cgggcacgct gcagcgcgtc ctcgacgcga
     2221 tcgaggcggc gtcccaccgg cctccgccca gcaccatggc cgtggacctg cccgcggacg
     2281 ccggtgagga gctggtccgg ctgctggtca ccgtccgcgg cgacacggtg accgtcaccg
     2341 gtggcccttc cgaccagggg ctgccccccg ggtgggcccg tcccctcgcg gaggcgctgg
     2401 cacagcgcgg catggccctg gacggcttcg cgtccggcca gacgcccgac cgccgccgcc
     2461 gaggcgagcc cgagcccgcc ccacgaggcc gtccaccggc cggccaggcc gtgcggccct
     2521 ccccgcgcgt ccacgacggc gccctgcgcc tgtaggagga agaccatgcc aatcgacggg
     2581 atcagtggca tcggcgggac cgccgagcct ccgaagtcgt ccgcggtcca gagcctcgga
     2641 ggcatggaca gcgacgcgtt cctcaagctt ctggtggcgc agatgcgcta tcagaacccc
     2701 atggcgccca cggatggggc cgccatgctg cagcagaccg ctcagttcac gcaggtggag
     2761 acgctcaagc agctcgccac cgcgcagaag gagctgctca gctaccacca ggcgtccgtg
     2821 gcctcgagcc tggtgggcaa ggaagtcacc gcggtcaagg acgacggcac cgaggtcacg
     2881 ggaacggtcg atgccatccg gttcaccagc gacggccccg tgctgcgcat gggcgaactg
     2941 gaactgagcc tcgacgccac ccgcgagatc cgcctggccg gcgccggcgg cgccacgtcc
     3001 gagtaacaca cctctcagaa ggagcttccc atgctgcgct cgatgttcgc cgccgtcagc
     3061 ggcctgcgct cgcaccagac catgatggac gtcgtcggca acaacgtcgc caacgtcaac
     3121 accgccggct tcaagtcgtc ccgcaccacg ttccaggacg cgctgaacca ggtcatccgc
     3181 gggtcgtcgg ccgtgtcgga cgaccgcgcc ggcaccaacc cgatgcagat cggcctgggc
     3241 tcgcaggtca ccgccgtgga ccaggtcttc ggccagggct cgatccagtt gaccggacgc
     3301 aacaccgacc tggcgatcca gggcgagggc ttcttcatga tgcgcatcgg cgagcagacg
     3361 ttctactcgc gcgccggcgc cttcggcctg gacgcgaacg gctcactcac gggccccggc
     3421 ggtgcccggg tgaccggctg gatgcccgac gccaacggcg cgatcgacac ccagcagccc
     3481 ctggagaccc tccagctgcc caccggtcag gtgatccagc cgcaggagag caacaacgtg
     3541 accgtcggcg gcaacctgcc ggccagcgcg gccaacggca cggcggtgaa cacgtcgatc
     3601 accatccacg accagcaggg cacgtcctac tcgctggtgc tcaccttcac gaaggtgggc
     3661 ccggacgagt gggacatgac ccagtcgctc gacggcgtgg cgttcggcgc cgcgcaggat
     3721 gtcttcttcg acccggcgac cggcgagccg ctgaacgccg cggccccggg cggcacggcg
     3781 ctggcggcgc tgacggtcga cccggccggc accgccggtg tgtgggacgg caacggcatc
     3841 gagatcaact tcgacgaggt gggtgtggag ctgcggcagt tcgccggggc gaccacgg
//
LOCUS       DATMKC010000394         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_4383, whole genome shotgun sequence.
ACCESSION   DATMKC010000394 DATMKC010000000
VERSION     DATMKC010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4383"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VNU01_11485"
     CDS             <1..471
                     /locus_tag="VNU01_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33281.1"
                     /translation="LPHPPELVAKAKTIVLTETVLAATVVAAVAGSLRSPGAPTASQL
                     TAIVAAGLTCTAVVVAGSLKMSVRRPHRADLRGPRDAVAPPGALALASARLAIPTALV
                     GIVLGGASQVELWWWPPLLAAPVLLLCVLSVMRSLRRYEDPFHRARIVQTVSSG"
     gene            614..1783
                     /locus_tag="VNU01_11490"
     CDS             614..1783
                     /locus_tag="VNU01_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWH33282.1"
                     /translation="MVLTPLLAAPAVAAPPSPSPTPAVDAALSAHLQQRAQRQAAELE
                     AGLAAQQVAAEQATAALEAYQAARRAADAAVRHSASQARLLVAAEKRTEQTRARLARY
                     VGSLYRTGMGNRQLAVYSSLMDARNPQQLFRGLGMVSRVGGNQNDAFLGLARAEEFQA
                     RAAARAERAAKDAAAAQQRAAAAKEAADQVVADAVAHVTRASAALAKTEQALALAQRR
                     EKLMASASLIARQRAAIPPAAIEGALAERPIPECKGAPTEGYPNGRLPLSALCPLWGT
                     SGQLLRWDAAAAFDAMSKEFGAHFGEPICVTDSYRSYPEQVAVALAKPDLAAVPGRSN
                     HGWGVALDLCGGIQSFTTDQHVWMVANSMAYGWFLPAWAQRGGSKPEPWHWEYAG"
     gene            1848..2393
                     /locus_tag="VNU01_11495"
     CDS             1848..2393
                     /locus_tag="VNU01_11495"
                     /inference="COORDINATES: protein motif:HMM:NF042550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6636 domain-containing protein"
                     /protein_id="HWH33283.1"
                     /translation="MTPHGRLRLAGLLCAVALATACNGQEPAPRAAGSPSATAAPSPS
                     TSPSPTTSPSDPAEPAVPTVLPPGAAVDFLSPTGNIHCSISSQGAVCEVRERSYEPPP
                     KPADCELDHGTMIGVGRRGPAAFLCHGDTGFGSPTPVLEYGRSTTNGLFMCRSSQEGM
                     SCEVSGGKHGFEISRDAYRVY"
BASE COUNT          321 a          964 c          891 g          305 t
ORIGIN      
        1 ctcccccacc cgcccgagct ggtggccaag gccaagacga tcgtgctgac cgagacggtg
       61 ctggccgcca cggtcgtcgc ggcggtcgcc gggtcgctgc ggagtccggg ggcacctacc
      121 gcgtcgcagc tgaccgccat cgtcgccgcc gggctgacct gcaccgctgt cgtcgtggcc
      181 ggctcgctga agatgtccgt acgccgtccg caccgcgccg acctgcgcgg cccgcgggac
      241 gccgtcgcgc cgcccggagc gctcgcgctc gccagcgccc gactggcgat cccgaccgcg
      301 ctggtcggga tcgtcctggg cggggcgagc caggtcgagc tgtggtggtg gccgccgctg
      361 ctggctgccc ccgtcctgct gctgtgcgtg ctgtccgtga tgcgcagcct gcgccggtac
      421 gaggacccgt tccaccgcgc ccgcatcgtg cagacggtct cgtccggctg acggaccggc
      481 tgactacttc gagccattcg aagcagaaac ggccccgcag gccacctgcc ctggcaccgt
      541 catgagttcg tcatgaagtc caggtcggag ggtccagcag gtgcgcaggt cttggcggac
      601 agccctcgcg gcagtggtcc tcacgccgct gctcgcggca cctgccgtgg ccgcccctcc
      661 ttcgccgtcg ccgacccccg cggtcgacgc cgcgctctcc gcgcacctgc agcagcgggc
      721 ccagcggcag gccgccgagc tcgaggccgg gctggccgcc cagcaggtcg cggccgagca
      781 ggccaccgcc gcgctggagg cctaccaggc cgcccggcgc gcggccgacg ccgccgtacg
      841 ccactccgcc tcgcaggcgc ggctgctggt cgccgcggag aagcggaccg agcagacgcg
      901 ggcgcgactg gcgcgctacg tcggctcgct ctaccggacc gggatgggca accggcagct
      961 ggccgtctac tccagcctca tggacgcgcg gaacccgcag cagctgttcc gcggcctcgg
     1021 catggtctcg cgggtcggcg gcaaccagaa cgacgccttc ctcggcctcg cccgggcgga
     1081 ggagttccag gcgcgggctg ccgcacgggc cgagcgcgcc gccaaggacg ccgcggcggc
     1141 ccagcagagg gccgccgccg ccaaggaggc ggccgaccag gtcgtcgccg acgccgtcgc
     1201 ccacgtgacc cgggccagcg ctgcgctggc caagaccgag caggcgctgg cgctggcgca
     1261 gcggcgcgag aagctgatgg cgtcggcgtc gctcatcgcc cggcagcgcg cagccatccc
     1321 gccggcagcc atcgagggcg ccctggccga gcgcccgatc cccgagtgca agggcgcgcc
     1381 gacggagggc tacccgaacg ggcgcctgcc gctcagcgcg ctgtgcccgc tgtggggcac
     1441 cagcgggcag ctgctgcgct gggacgccgc ggccgccttc gacgcgatga gcaaggagtt
     1501 cggcgcgcac ttcggcgagc cgatctgcgt caccgactcc taccgcagct atcccgagca
     1561 ggtcgcggtg gcgctggcca agcccgacct cgcggccgtc cccggacgca gcaaccacgg
     1621 ctggggcgtc gcgctcgacc tgtgcggcgg catccagagc ttcacgaccg accagcacgt
     1681 ctggatggtc gccaactcca tggcgtacgg ctggttcctg ccggcgtggg cgcagcgcgg
     1741 cggctccaag cccgagccgt ggcactggga gtacgcaggc tgagctcccg cacccggccc
     1801 acgacgcacc cgctgccggc acccgccgct gctgtagaac gggcgccatg accccgcacg
     1861 gacgtctccg cctggccggt ctgctctgcg cggtggcgct ggccacggcg tgcaacggtc
     1921 aggagccggc gccccgggcg gcgggctcgc cgtcggccac ggctgcgccc tcgcccagca
     1981 cgtcgccctc ccccaccaca tcgccctccg accccgccga gcccgcggtg ccgacggtgc
     2041 tgcctccggg cgccgcggtg gacttcctgt caccgacggg caacatccac tgcagcatca
     2101 gcagccaggg cgcggtgtgc gaggtcaggg agcggtcgta cgagccaccg ccgaagcctg
     2161 ccgactgcga gctcgatcac ggcacgatga tcggcgtggg acgccggggg ccggcggcct
     2221 tcctctgcca cggcgacacc gggttcggct cccccacacc ggttctggag tacgggcgga
     2281 gcaccaccaa cggcctgttc atgtgccgca gcagccagga gggcatgtcc tgcgaggtct
     2341 ccgggggcaa gcacggcttc gagatctccc gcgacgccta ccgggtctac tgaggccggg
     2401 agctcagaag ggcaccgggg tcccgagcag ccccggctgc gggtcgcgcc cggacaccga
     2461 gcggcagaag tcgattgcgt c
//
LOCUS       DATMKC010000395         5306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_59881, whole genome shotgun sequence.
ACCESSION   DATMKC010000395 DATMKC010000000
VERSION     DATMKC010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59881"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VNU01_11500"
     CDS             <1..1052
                     /locus_tag="VNU01_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33284.1"
                     /translation="LAGRTLVPVWHETDVTSGNFSTQSQAACATFTEDHEVFAAMSSA
                     VGGNDTLAACMAQKATPLVERNLWLFDTPYYRQFAGYLYQPGKMRADRWAAAYADGLV
                     ESGYTKDAKLGLVRFDGPVFQRVADGAFKPALARHGVRLTDEAVLSTPGGISDFGRMS
                     AEINNAIVRFRAAGVTHVVFIENAGILPFFWMPQADSSGYRPRYGIASNSIPATMAGQ
                     ALASWLANAVVVGWTPPNDVPAAHHPGGNPAWARCNEIMKAAGTSGAAGFYTQSYCDA
                     FFFLKAVADRAPSLDAPGFRAGVESLGGAYNSPFTFATRFGPGRHDGAAQWRTGLFHD
                     DCACFRYTGPLRAAP"
     gene            1049..1834
                     /locus_tag="VNU01_11505"
     CDS             1049..1834
                     /locus_tag="VNU01_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWH33285.1"
                     /translation="MSAPLRLERDGGVAELVLDRPEAANSIDLGMARALLDAAIRLGQ
                     EPAVRVVLLRGEGARFCGGGDLKAFATTGDGLAGHLEEVTANLHAAIARLARLDAPVI
                     AAVHGAAAGAGFSLACAADLLLAAEDTLFVPAYAKAGLSPDGSLSATLPRLVGPRRAL
                     ELLLTGRPLSAREALDWGIVTAVHPDGELLDAARELAARLAEGPTRAYGAAKRLVRSG
                     WELPLEAQMERESAELAAMARGRDGREGVAAFTERRPAVFTGE"
     gene            complement(1821..2630)
                     /locus_tag="VNU01_11510"
     CDS             complement(1821..2630)
                     /locus_tag="VNU01_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HWH33286.1"
                     /translation="MSDAGAMQALCADLRAEQDELAALLADLEERCWDTPTPAAGWNV
                     RDQIGHLAHGEELAALAATDPDGFQAELARLMGDLDGRIGAVLARARASDGPLLLAWW
                     LGARERTLAALAGQDERERIAWAGPAMSARSFAAARLMETFAHGQDIRDALGAPPVPS
                     PRLRHVAELGVRTRAFAYLLRELDAPATPLRVELDAPDGGTWVWGEDDAPDRVSGPAL
                     DFCLVVTRRRHPADTRLDAHGPHAAEWLAIAQCYLGPPDEGRPAGSFPTPP"
     gene            complement(2627..3871)
                     /locus_tag="VNU01_11515"
     CDS             complement(2627..3871)
                     /locus_tag="VNU01_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH33287.1"
                     /translation="MDFRDTPEQAAFRAELRAWLDAHAPRRPGDVPESLGAVIRREGA
                     DDAVEASRAWQATLHSGGWGAITWPREYGGRDAGVVEAYIFADELRRSDVPPSVFAIT
                     LGMVGPTIIAHGTAEQKARYLERMVDGSELWCQLWSEPGAGSDLAGLATRAEGDPATG
                     WRLTGQKVWTSWAHHARWGLVLARTNPDAPKHAGLSCFIVDMHAPGVTVRPLRQMTGQ
                     SHFNEVWFEDAVVPPENLVGPVDQGWRVAMTTLMNERYTAGTSSVGVLSPDPLIALAR
                     RTERGGRPAAADPVVRQDLARVWIAARLFELTLYRSLTRAAKGQEPGPEGSVMKLSAT
                     GLATELGEAGLRVLGAGGALDGDDAEDRGEWVQARLFAAGFRIAGGTDEIQKTILGER
                     VLGLPPEPQPAKGASFKSQARQ"
     gene            3983..>5306
                     /locus_tag="VNU01_11520"
     CDS             3983..>5306
                     /locus_tag="VNU01_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH33288.1"
                     /translation="MGKGRGVESHLVSRRDLDFLLYEWLEVESLVERERYAAHDRDTM
                     DAALELAERIATERFAPHNKTADEHEPTFDGERVHLTPEVKQAVDAYAAAGFIGAVLD
                     ERLGGMQLPHTVAQACMAWFQAANVGTTGYPFITAAAVNMLAAHGSPEQVDTYLGPMV
                     SGRFLGTMALSEPQAGSSLSDIVLRAEPRGDGSYRLFGNKMWISCAEHELSENIVHIV
                     LARIPGSPPGVKGISMFIVPKFLVQPDGSLGERNDVVVAGLNHKMGYRGTVNTLLNFG
                     EGRHTPGGEPGAVGELVGEPNRGLAFMFHMMNEARVGVGLGATALGYTGYLKSLRYAR
                     ERTQGRPVAGKDPLAPMVPIVEHADVRRMLLAQKAYVEGALALILYCAKLVDDEKTAE
                     TVEEREAAHLLLDTLTPIAKSWPSQWCLEANSLAIQIHGGYGYTREYDV"
BASE COUNT          675 a         1943 c         2004 g          684 t
ORIGIN      
        1 ggctggccgg gcgcacgctg gtgccggtgt ggcacgagac cgacgtcacg tccggcaact
       61 tctccacaca gtcgcaggcg gcctgcgcca cgttcaccga ggaccacgag gtgttcgccg
      121 cgatgtccag cgccgtgggc ggcaacgaca ccctggctgc ctgcatggcg cagaaggcca
      181 cgccgctggt ggagcgcaac ctgtggctgt tcgacacgcc gtactaccgc cagttcgccg
      241 gctacctgta ccagccgggc aagatgcgcg ccgaccgctg ggcggccgcc tacgccgacg
      301 ggctggtcga gtcgggctac accaaggacg ccaagctcgg gctggtgcgc ttcgacgggc
      361 cggtgttcca gcgcgtcgcc gacggcgcgt tcaagccggc gctggcgcgc cacggtgtgc
      421 ggctcaccga cgaggccgtg ctgtccacgc cgggcggcat ctccgacttc gggcgcatga
      481 gcgccgagat caacaacgcg atcgtgcgct tccgggccgc cggggtcacc cacgtggtgt
      541 tcatcgagaa cgccggcatc ctgccgttct tctggatgcc gcaggccgac agctccggct
      601 accggccgcg ctacggcatc gccagcaact ccatccccgc gaccatggcg gggcaggcgt
      661 tggcgagctg gctcgccaac gccgtggtgg tcggctggac cccgcccaac gacgtgccgg
      721 cggcgcacca tccgggcggt aacccggcct gggcgcgctg caacgagatc atgaaggccg
      781 cgggcaccag cggcgcggcg ggcttctaca cccagtccta ctgcgacgcg ttcttcttcc
      841 tgaaggcggt cgccgaccgt gcgccgtcgc tggacgcccc cgggttccgg gcgggcgtgg
      901 agagcctggg cggcgcctac aactcgccgt tcacgttcgc cacgcgcttc ggaccgggcc
      961 gccacgacgg cgccgcgcag tggcgcacgg gcctgttcca cgacgactgc gcctgcttcc
     1021 gctacaccgg tccgctgcgg gccgcgccgt gagcgcgccg ctgcggctgg agcgcgacgg
     1081 cggtgtcgcc gagctggtgc tggaccgccc cgaggcggcc aacagcatcg atctcggcat
     1141 ggcccgcgcg ctgctggacg ccgccatccg cctcggccag gaaccggccg tccgcgtggt
     1201 cctgctgcgg ggcgagggcg cgcgcttctg cggcggcggc gacctcaagg cgttcgccac
     1261 caccggcgac gggctggccg ggcacctgga ggaggtgacc gcgaacctgc acgcggcgat
     1321 cgcccgcctg gcccgcctgg acgcgccggt gatcgcggcg gtgcacggcg cggcggccgg
     1381 cgcggggttc agcctggcgt gcgcggcgga cctgctcctg gccgccgagg acacgctgtt
     1441 cgtgccggcg tacgccaagg ccggcctgtc gccggacggc tcgctcagcg ccacgctgcc
     1501 gcggctggtg gggccgcgcc gggcgctcga gctgctgctg accggcaggc cgctgtcggc
     1561 gcgggaggcg ctggactggg gcatcgtcac cgcggtgcat cccgacggcg agctgctcga
     1621 cgccgcgcgc gagctggcgg cacggctggc cgaggggccg acacgcgcct acggcgccgc
     1681 caagcggctg gtgcgctccg gctgggagct gccgctggag gcgcagatgg agcgcgagtc
     1741 cgccgagctg gccgccatgg cgcgcggccg ggacgggcgc gagggcgtgg ccgcgttcac
     1801 cgagcggcgc ccggcggtgt tcacggggga gtagggaagc tccccgcggg gcggccctcg
     1861 tccggcgggc ccaggtagca ctgggcgatg gcgagccatt cggcggcgtg cggaccgtgc
     1921 gcgtccagcc gcgtgtcggc cgggtgccgc cggcgcgtga ccaccaggca gaaatccagg
     1981 gccgggccgc tgacgcggtc cggcgcgtcg tcctcgcccc agacccaggt gccgccgtcc
     2041 ggcgcgtcga gctccacgcg caggggcgtc gccggcgcgt ccagctcccg cagcaggtag
     2101 gcgaacgcgc gggtgcgcac acccagttcg gccacgtgcc gcagccgcgg gctcggaacc
     2161 ggcggcgcgc cgagcgcgtc gcggatgtcc tggccgtggg cgaaggtctc catcagccgg
     2221 gcggcggcga agctgcgcgc gctcatcgcc ggccccgccc aggcgatgcg ctcgcgctcg
     2281 tcctggccgg ccagcgccgc cagggtgcgc tcgcgcgcac cgagccacca cgcgagcagc
     2341 agcggcccgt cggaggcgcg ggctcgggcc aggaccgcgc cgatgcggcc gtccaggtcg
     2401 cccatgaggc gcgccagctc ggcctggaag ccgtcagggt cggtggcggc gagcgcggcc
     2461 agctcctcgc cgtgcgccag gtgcccgatc tggtcgcgca cgttccatcc ggccgcggga
     2521 gtcggcgtgt cccagcagcg ctcctcgagg tcggccagca gcgccgcgag ctcgtcctgc
     2581 tcggcgcgca ggtccgcgca cagcgcctgc atggccccgg cgtcgctcac tggcgcgcct
     2641 gcgacttgaa cgaggcgccc ttggcgggct gcggctccgg cggcaggccc aggacccgct
     2701 cgcccaggat ggtcttctgg atctcgtcgg tgccgccggc gatgcggaag ccggcggcga
     2761 acagccgcgc ctgcacccac tcgccccggt cctccgcgtc gtcgccgtcc agcgcgccgc
     2821 cggcgcccag gacgcgcagg cccgcctcgc ccagctcggt ggccaggccg gtcgccgaca
     2881 gcttcatgac cgagccctcc gggcccggct cctggccctt cgcggcgcgc gtgaggctgc
     2941 ggtacagggt gagctcgaac aggcgggcgg cgatccacac gcgcgcgagg tcctggcgca
     3001 ccaccgggtc cgcggcggcc ggccggccgc cgcgctcggt gcgccgggcc agcgcgatga
     3061 gcgggtcggg cgacagcacc ccgacgctgg acgtgcccgc cgtgtagcgc tcgttcatga
     3121 gcgtggtcat cgccacccgc cacccctggt cgacggggcc gacaaggttc tccggtggca
     3181 ccacggcgtc ttcgaaccag acctcgttga agtgcgactg gcccgtcatc tggcgcagcg
     3241 ggcgcacggt gacgccgggc gcgtgcatgt ccacgatgaa gcaggacagc cccgcgtgct
     3301 tcggggcgtc cgggttggtg cgcgccagca ccaggcccca gcgcgcgtgg tgcgcccagc
     3361 tcgtccagac cttctggccg gtcaggcgcc agccggtagc cgggtcgccc tccgcgcgcg
     3421 tggccagccc ggccaggtcc gagcccgcgc ccggctccga ccacagctgg caccacagct
     3481 ccgagccgtc caccatgcgc tccaggtaac gggccttctg ctcggcggtg ccgtgggcga
     3541 tgatcgtcgg tcccaccatg cccagggtga tcgcgaagac gctgggcggg acgtcggagc
     3601 ggcgcagctc gtcggcgaag atgtaggcct cgaccacgcc ggcgtcgcgg ccgccgtact
     3661 cgcgcggcca cgtgatggcg ccccagccgc cgctgtgcag cgtcgcctgc caggcgcgcg
     3721 aggcctccac ggcgtcgtcg gcgccctcgc ggcggatcac cgcgcccagg ctctccggca
     3781 cgtcgccggg gcggcgcggc gcgtgggcgt ccagccaggc gcgcagctcg gcacggaacg
     3841 cggcctgctc gggcgtgtct ctgaagtcca tcgcggctcc ggtgggcgtc cgccgcgcgg
     3901 cgcgggtggc ggcgtgtgta aattcagaat aacatcctgc cgtgccgcgc gcgagtgcgc
     3961 gtccggacgc ctgggcggcg gcgtgggaaa gggcagggga gtggagtcgc acctggtatc
     4021 gcgccgtgac ctggacttcc tgctgtacga gtggctggag gtggagtcgc tggtcgagcg
     4081 ggagcgctac gccgcccacg accgtgacac catggacgcg gcgctggagc tggccgagcg
     4141 catcgccacc gagcggttcg cgccgcacaa caagaccgcc gacgagcacg agcccacgtt
     4201 cgacggcgag cgcgtgcacc tgaccccgga ggtcaagcag gccgtcgacg cctacgcggc
     4261 ggcgggattc atcggcgccg tgctggacga gcgcctcggc ggcatgcagc tgccgcacac
     4321 cgtggcgcag gcctgcatgg cgtggttcca ggcggccaac gtcggcacca ccggctaccc
     4381 gttcatcacc gccgccgccg tcaacatgct ggccgcgcac ggctccccgg agcaggtcga
     4441 cacctacctc gggccgatgg tgtccggccg cttcctgggc accatggcgc tgtcggagcc
     4501 gcaggccggc tcgtcgctgt cggacatcgt gctgcgcgcg gagccgcgcg gggatggcag
     4561 ctaccggctg ttcggcaaca agatgtggat ctcgtgcgcg gagcacgagc tgagcgagaa
     4621 catcgtgcac atcgtgctgg cgcgcatccc cggctcgccg ccgggggtga agggcatcag
     4681 catgttcatc gtgccgaagt tcctggtcca gccggacggc tcgctgggcg agcgcaacga
     4741 cgtggtcgtg gcggggctca accacaagat gggctaccgc ggcacggtca acacgctgct
     4801 caacttcggc gagggccgcc acacgccggg cggcgagccg ggcgcggtgg gcgagttggt
     4861 gggcgagccc aacaggggcc tcgcgttcat gttccacatg atgaacgagg cgcgcgtggg
     4921 tgtgggtctg ggcgcgacgg cgctgggtta cacgggctac ctcaagtcgc tgcgctacgc
     4981 gcgggagcgc acgcagggcc ggccggtggc gggcaaggac cccctcgcgc cgatggtgcc
     5041 gatcgtcgag cacgccgacg tgcgccgcat gctgctggcg cagaaggcct acgtcgaagg
     5101 ggcgctggcg ctgatcctgt actgcgccaa gctggtcgac gacgaaaaga ccgccgagac
     5161 ggtcgaggag cgcgaggccg cgcacctgct gctggacacg ctgacgccga tcgccaagag
     5221 ctggccgtcg cagtggtgcc tggaggcgaa ctcgctggcg atccagatcc acggcggcta
     5281 cggctacacg cgcgagtacg acgtcg
//
LOCUS       DATMKC010000396         3079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_59942, whole genome shotgun sequence.
ACCESSION   DATMKC010000396 DATMKC010000000
VERSION     DATMKC010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59942"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1601)
                     /gene="glpK"
                     /locus_tag="VNU01_11525"
     CDS             complement(111..1601)
                     /gene="glpK"
                     /locus_tag="VNU01_11525"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HWH33289.1"
                     /translation="MARILTIDAGTTGVTALVVDETGGVLTRGYREFPQHFPRPGWVE
                     HDADDIWAATLEACGDALRDSATAADRLAAIGITNQRETAVLWDRRTLRPAARAIVWQ
                     DRRTAGRCDELRDAGHAPFVRERTGLVVDAYFSGTKLAWLLDHTEGARDRAARGELAF
                     GTVDSWLVARLTGGKVHATEPSNASRTMLYDIRKGAWSQELCDLLDVPMGVLPEVRPS
                     AGSFGATDPDAFHGAAVPIAGIAGDQQAALFGQGCWTPGMSKNTYGTGSFVLLNTGGE
                     AAASDRLLTTVAWDLGDGLVYALEGSIFVTGAAIQWLRDGLGVIADAAETGPLAESVP
                     DTGDVYLVPAFTGLGAPWWDPYARGAIVGITRGTTKAHLARAAVEAMGHQTRDVVEVM
                     QADSGVTLDELRVDGGACVMDLLCQFQADVLGVPVLRPTVRETTAMGAAYLAGLGSGV
                     WGSTAELEQVWKLERRFTAQWDAERRDAAHGRWRQALERARGWATD"
     gene            1732..2766
                     /locus_tag="VNU01_11530"
     CDS             1732..2766
                     /locus_tag="VNU01_11530"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HWH33290.1"
                     /translation="MATRIGILTGGGDCPGLNAVIRAVVRRADAFNMAVWGFRNGWQG
                     VLEGTAEPLTLDSTRGILHRGGTILGTSRVDPIATDGGVDRIRDALEVHRLDGLVVVG
                     GEGTLSAATLLSDEHGLPVVGVPKTIDNDLGATQFTIGFSTALDVATEAIDRLHTTAE
                     SHNRVMLLEVMGRHVGWIATYAGIAGGADAILIPEQPFDVRRIAKHLKHRHGMGRTFS
                     IVVVAEGATPQPGTLDLPEYERDEFGRPVLGGIANHIAPVIEDLTGYSTRVTILGHVQ
                     RGGSPNAFDRVLGSNMGVLAVDLARAGEWGKMTAMQGNAVVPVDLREATRELKRVPQS
                     LYDVAEVFFG"
BASE COUNT          382 a         1161 c         1114 g          422 t
ORIGIN      
        1 gtggtgcgcg ccgtcgccga cggccgcctg agccaggcgc gcctggccga ggccgccggg
       61 cgcatcctga acctcaaggg cgtcgaggcc cggccggtgg tctgcgccgc ttagtccgtc
      121 gcccagccgc gcgcgcgttc cagggcctgc cgccagcggc cgtgcgcggc gtcacgccgc
      181 tcggcgtccc actgtgcggt gaagcggcgc tccagcttcc agacctgctc cagttccgcc
      241 gtcgagcccc acacaccgga gcccagcccg gcgaggtagg cggcacccat cgcggtggtc
      301 tcgcgcacgg tcggccgcag caccggcacg ccgagcacgt ctgcctggaa ctggcacagc
      361 aggtccatga cgcacgcgcc gccgtccacg cgcagctcgt ccagggtgac gccggagtcg
      421 gcctgcatga cctccacgac gtcgcgcgtc tggtggccca tcgcctccac ggccgcgcgc
      481 gccaggtgcg ccttggtggt gccccgcgtg atcccgacga tcgcgcccct ggcgtagggg
      541 tcccaccacg gcgcgccgag cccggtgaac gccgggacca ggtacacgtc gccggtgtcg
      601 gggaccgact ccgccagggg accggtctcc gccgcgtcgg cgatcacgcc gaggccgtcg
      661 cgcagccact ggatcgccgc gcccgtgacg aagatggagc cctccagcgc atagacgagg
      721 ccgtcgccca ggtcccaggc caccgtggtc agcagccggt cgctggccgc ggcctcccca
      781 ccggtgttga gcagcacgaa cgaccctgtg ccgtaggtgt tcttcgacat gccgggcgtc
      841 cagcagccct ggccgaacag cgcggcctgc tggtctccgg cgatgccggc gatgggcacc
      901 gcggcgccgt ggaaggcgtc ggggtccgtg gcgccgaacg agcccgccga gggccgcacc
      961 tccggcagca cgcccatcgg gacgtcgagc aggtcgcaca gctcctggct ccacgcgccc
     1021 ttgcggatgt cgtacagcat cgtgcgggac gcgttggatg gctccgtggc gtggaccttc
     1081 ccgccggtga gccgcgccac cagccaggag tccaccgtgc cgaaggccag ctcgccgcgc
     1141 gccgcccggt cccgcgcgcc ctcggtgtgg tccagcagcc acgccagctt ggtgccggag
     1201 aagtacgcgt cgaccaccag gccggtgcgc tcccggacga agggcgcatg cccggcgtcg
     1261 cgcagctcgt cgcagcgccc ggccgtgcgg cggtcctgcc acacgatcgc gcgtgccgcc
     1321 ggccgcaggg tgcgccgatc ccacagcacc gcggtctcgc gctggttggt gatgccgatc
     1381 gcggccaggc ggtccgcggc ggtggccgag tcccgcagcg cgtcgccgca cgcctccagc
     1441 gtcgccgccc agatgtcgtc ggcgtcgtgc tccacccacc cgggccgcgg gaagtgctgc
     1501 gggaactcgc ggtagccgcg cgtgagcacc ccgccggtct cgtccacgac cagggccgtc
     1561 acccccgtgg tgccggcgtc gatggtgagg atcctggcca cgcgtcctcc tgggtcgccc
     1621 gtgcgctgcg ccgcacccgg agggcgccgg cgatgtccgc cgcaagtcta ggaaagggac
     1681 gagcgcctag gatgccttgc gcggctggcg gactggcagg aaaggactct cgtggcgacc
     1741 cgcatcggca tcctcaccgg cggaggggac tgtcccggcc tcaacgcggt gatccgggcc
     1801 gtggtgcggc gcgcggacgc gttcaacatg gcggtatggg gcttccgcaa cggctggcag
     1861 ggcgtgctcg agggcacggc ggagccgctc acgctggact ccacccgggg catcctccac
     1921 cgcggtggga cgatcctcgg caccagccgc gtcgacccga tcgccaccga cggcggtgtg
     1981 gaccgcatcc gcgacgcgct ggaggtgcac cggctcgacg gcctcgtggt ggtcggcggc
     2041 gagggcacgc tgtccgcggc gaccctgctg tccgacgagc acggcctgcc ggtggtcggc
     2101 gtgcccaaga ccatcgacaa cgacctcggc gccacgcagt tcacgatcgg cttctccacg
     2161 gccctcgacg tggcgaccga ggcgatcgac cgcctgcaca ccacggccga gagccacaac
     2221 cgcgtcatgc tgctggaggt gatgggccgc cacgtcggct ggatcgccac ctacgccggc
     2281 atcgcgggcg gcgccgacgc gatcctcatc ccggagcagc cgttcgacgt ccggcgcatc
     2341 gccaagcacc tcaagcaccg ccacggcatg ggccgcacgt tctccatcgt ggtggtggcc
     2401 gagggcgcca ccccgcagcc gggcaccctg gacctgcccg agtacgaacg cgacgagttc
     2461 ggccgccccg tcctcggcgg catcgcgaac cacatcgccc cggtgatcga ggacctcacc
     2521 ggctactcca cccgcgtcac gatcctcggc cacgtccagc ggggcggcag ccccaacgcg
     2581 ttcgaccgcg tgctcggctc caacatgggc gtgctcgccg tggacctggc ccgcgcgggg
     2641 gagtggggca agatgaccgc gatgcagggc aacgccgtcg tgcccgtcga cctgcgcgag
     2701 gcgacgcgcg agctcaagcg cgtgccgcag agcttgtacg acgtggccga ggtgttcttc
     2761 ggctagccgc gcgtgcgtag ccgcgcgtgc gcgcgtgtgc ggtcgcgtgg gcccggaccc
     2821 ctcctcgctc ctcgcccggc ttatgtggcg gtgtcgggcg tcggcggcca tgttgcggcc
     2881 gtgcgtgtgt ggcgccgcgg gccgggctcg tcctcgtcgg gctccggttc ccacgccgac
     2941 cgccaccccg cccgcctgtt ggcggtgtgt gggcgggctt cgcgccccac ctggcgggtg
     3001 gggtggcggg tcagccggta tgggcgagcg ctggtttctc tatgttgttg tcaaggtgca
     3061 gtggttccgg tggtgcggg
//
LOCUS       DATMKC010000397         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_108444, whole genome shotgun sequence.
ACCESSION   DATMKC010000397 DATMKC010000000
VERSION     DATMKC010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108444"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VNU01_11535"
     CDS             <1..365
                     /locus_tag="VNU01_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33291.1"
                     /translation="CWFGGPTGINNDLTGKTVNIHHYGHGLAVGDVLPARTSVAYGMR
                     DKNEVTALGVVIPGDSGGAAISSDGRAVGVVVSVGASFQASPLTFGTVGITRLRPQVT
                     RAEQVLKQRLVMATAPLL"
     gene            415..615
                     /locus_tag="VNU01_11540"
                     /pseudo
     CDS             415..615
                     /locus_tag="VNU01_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            complement(800..1270)
                     /locus_tag="VNU01_11545"
     CDS             complement(800..1270)
                     /locus_tag="VNU01_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33292.1"
                     /translation="MRPEELEAVCQEAARGLLEREGRPVPAAVVLPLPEATRVVTLPG
                     FPEDDATRAHALADFATDLMRPANAPAYGFVAEAEQADGLPLLVVVYGARGLTPRITA
                     AALHEHGPGEWTPAEDLHPDALPFLRGLQAAADAADPSRPVGGSALPGWPPPGG"
     gene            complement(1270..1779)
                     /locus_tag="VNU01_11550"
     CDS             complement(1270..1779)
                     /locus_tag="VNU01_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33293.1"
                     /translation="MRRAAWLVAVLALALGACGEPEVPLQVPPRGADDVVIDDARILD
                     VDALARDLYAFRHQGWDPVLVTFEAEGASMGMADRAGRKVIEAWGADLAIVAVAEPGD
                     FRATGSERRRFFGLFARDVREVPRDVRERIVEELVPPIAARNDWTEAFHVAVTELAGA
                     LGEVDEDIR"
     gene            complement(1772..2638)
                     /locus_tag="VNU01_11555"
     CDS             complement(1772..2638)
                     /locus_tag="VNU01_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HWH33294.1"
                     /translation="MAHRMLLDTSSLFYRAYFALPTSIKAPDGTPVNALRGYLDMTAR
                     LLHNHRPDEVLHVRDDEECPAPRLAAFPAYKQHRPPMPEGLPEQFALLWPLLAALGMD
                     MVDSAGWEADDAIGTLCAQAAPGDRVDVVTGDRDLIQLVRDADPPVRLLFTLKGVSEL
                     GVFDEAAVRAKYGMPAARYAEFATLRGDPSDGLPGVKGVGEKTALQLVTAYPSLEGLY
                     ADLLSQPPGLARKLSDARDYVTAMRDVVPVRRDVALRRAPGTRDDARLDELAAAWNLE
                     APLRRLREVLDA"
     gene            2745..3485
                     /locus_tag="VNU01_11560"
     CDS             2745..3485
                     /locus_tag="VNU01_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH33295.1"
                     /translation="MTSTLIWITGASSGIGAALAETVPFDDARVFDISRSGGTPGTEH
                     VPADLSDPSAWAAVEAHLASQIGAFGGERVVLVHNAGTLEPMGFAGEVDSEAYRRNVV
                     LNSGAPQALGHAFLKAAAAFRGEAHLLMLSSGAATSPYPGWSSYCAGKAALDMWCRTA
                     GEEQRLRAERGQPSARVVSVAPGVVATPMQERIRAMSPQDFPGVAKFVGLHERGELKD
                     PADAARGIWGLLERELPSGSVVDLRTLS"
BASE COUNT          444 a         1388 c         1312 g          446 t
ORIGIN      
        1 tgtgctggtt cggcggcccc accgggatca acaacgacct caccggcaag accgtcaaca
       61 tccaccacta cggccacggc ctggcggtgg gcgacgtgct gccagcccgc acctccgtgg
      121 cctacggcat gcgcgacaag aacgaggtga ccgccctggg cgtggtcatc ccgggcgact
      181 ccggcggcgc ggcgatcagt tccgacgggc gcgcggtggg cgtggtcgtc tccgtgggcg
      241 cgagcttcca ggccagcccc ctgaccttcg gcaccgtggg catcacccgg ctgcggcccc
      301 aggtcacccg cgcggagcag gtgctcaagc agcgcctcgt gatggccacc gcgccgctgc
      361 tgtaggcccc gccccgcggg tcaccgccgc cgggccgcta gcctggtcgg cgtcatgcag
      421 cccgaccact acgccacgct cggtgtgcca tcgacggccc cgctcccgga gattcgcgcc
      481 gcgtacctgc gggcgatgcg cgcctgccac ccggacctgc gtcccggcga cctcgccgct
      541 ggcgaggcgg cccgccgcgc caacctcgcc tggcaggtgc tcggcgaccc gcagcggcgc
      601 gccgcctacg accgccagcg gcgcgcgcgc ggccttgtgg gcctcccgtc ggcgcccgcc
      661 catccggggc aggccgccta cctggcgcgg cgccgcgcgt acggccgcgc gttccaccag
      721 gcgtgcctga agggcgcggc ggccgtcttc gcgctcggcc tggtgctgct cgcgttcacc
      781 gccggctgag cgtcgccggt cagccccccg gcgggggcca gccgggcagg gcgctgccgc
      841 ccaccggccg cgaggggtcg gcggcgtccg cggcggcctg caggccgcgc aggaacggca
      901 gcgcgtcggg gtgcaggtcc tctgcgggcg tccactcgcc cgggccgtgc tcgtgcagcg
      961 ccgcggccgt gatgcgcggg gtcaggccgc gcgcgccgta caccaccacc agcagcggca
     1021 gcccgtccgc ctgctccgcc tcggcgacga agccgtaggc gggggcgttc gcagggcgca
     1081 tcaggtccgt ggcgaagtcg gccagcgcgt gcgcgcgcgt ggcgtcgtcc tccgggaagc
     1141 ccggcagcgt cacgacgcgg gtcgcctccg gcagcggcag gacgaccgcg gccggcacgg
     1201 gacggccctc gcgctccagc aggccgcgcg ccgcctcctg gcagaccgcc tccagctcct
     1261 ccggccgcat cagcggatgt cctcgtcgac ttccccgagc gcgccggcga gctccgtcac
     1321 ggcgacgtgg aacgcctccg tccagtcgtt gcgcgcggcg atgggcggca ccagctcctc
     1381 gacgatgcgc tcgcgcacgt cgcgcgggac ctcgcgcacg tcgcgggcga acagtccgaa
     1441 gaagcggcgg cgctccgagc cggtggcgcg gaagtcgccg ggctccgcca cggcgacgat
     1501 cgccaggtcc gcaccccacg cctcgatgac cttgcggccg gcgcgatcgg ccatccccat
     1561 gctggcgccc tcggcctcga acgtgaccag caccgggtcc cagccctggt ggcggaatgc
     1621 gtacagatcc cgcgccagcg cgtcgacgtc gaggatgcgg gcgtcgtcga tgacgacgtc
     1681 gtcggcgccg cgcggcggca cctgcagggg cacctccggc tcgccgcagg cgcccagcgc
     1741 caatgccagc acggccacca gccacgcggc cctacgcatc gagcacctcc cgcaggcgcc
     1801 gcagcggcgc ctcgaggttc cacgccgccg cgagctcgtc cagccgcgcg tcgtcgcgcg
     1861 tgcccggcgc ccggcgcagc gccacgtcgc ggcgcacggg caccacgtcg cgcatcgcgg
     1921 tcacgtagtc gcgcgcgtcg gacagcttgc gcgccagccc agggggctgg gacagcaggt
     1981 cggcgtacag cccctccagg gacgggtagg ccgtgacgag ctgcagcgcg gtcttctccc
     2041 ccaccccctt gacgccgggc aggccgtcgg acgggtcgcc gcgcagggtg gcgaactccg
     2101 cgtagcgcgc ggccggcatg ccgtacttgg cccgcaccgc cgcctcgtcg aacacgccga
     2161 gctcgctcac gcccttcagg gtgaacagca ggcgcacggg cgggtcggcg tcgcgcacga
     2221 gctggatcag gtcgcggtcg ccggtgacca cgtcgacgcg gtcgccgggc gcggcctggg
     2281 cgcacagggt gccgatggcg tcgtcggcct cccagccggc gctgtcgacc atgtccatgc
     2341 ccagcgccgc cagcaggggc cacagcagcg cgaactgctc cggcaggccc tccggcatgg
     2401 gcggacggtg ctgcttgtac gcggggaagg ccgccaggcg cggggccggg cactcctcgt
     2461 cgtcgcgcac gtggaggacc tcgtccggac ggtggttgtg cagcagccgc gcagtcatgt
     2521 ccaggtagcc gcgcagcgcg ttgaccggcg tgccatcggg cgccttgatg gacgtcggca
     2581 gcgcgaagta ggcccggtag aacaggctcg aggtgtccag cagcatgcgg tgggccatat
     2641 ggcgcgccag cctaaccccc cactccaacc tggaggatcc cgctcacatt ggaggatcac
     2701 ttggcgtatg cggcagcgat cttcccaaac tctaggatcg ctgcatgact tcgaccctga
     2761 tctggatcac cggcgcctcg tccggcatcg gcgccgcgct ggccgagacc gtcccgttcg
     2821 acgacgcgcg cgtgttcgac atctcccgct ccggcggcac gccgggcacc gagcacgtgc
     2881 cggccgacct gtccgatccc agcgcgtggg ccgccgtcga ggcgcacctg gccagccaga
     2941 tcggcgcgtt cggcggcgag cgggtggtcc tggtgcacaa cgccggcacg ctggagccga
     3001 tgggcttcgc cggcgaggtg gactcggagg cctaccgacg caacgtggtg ctgaactccg
     3061 gcgcgcccca ggcgctcggc cacgccttcc tgaaggcggc cgcggcgttc cgcggcgagg
     3121 cgcacctgct gatgctgtcc tcgggtgccg ccaccagccc ctaccccggc tggtccagct
     3181 actgcgccgg caaggcggcg ctggacatgt ggtgccggac ggccggcgag gagcagcggc
     3241 tgcgggcgga gcgcggccag ccgagcgcgc gcgtggtctc ggtggcgccc ggcgtggtcg
     3301 ccaccccgat gcaggagcgc atccgcgcga tgtcgccgca ggacttcccc ggcgtcgcca
     3361 agttcgtcgg cctgcacgag cggggcgagc tcaaggaccc cgccgacgcg gcgcgcggca
     3421 tctggggact gctggagcgc gagctgccct cggggtccgt ggtggacctg cgcacgctgt
     3481 catgaacgcg ccgttcgccc cgccggtcag ccccatggag gcgaaggtcc gcgacgacct
     3541 gccggccccg cccggctggg cctacgagcc gaagtgggac ggtttccgca
//
LOCUS       DATMKC010000398         8050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_31065, whole genome shotgun sequence.
ACCESSION   DATMKC010000398 DATMKC010000000
VERSION     DATMKC010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31065"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VNU01_11565"
     CDS             <1..642
                     /locus_tag="VNU01_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33296.1"
                     /translation="GMVLTVVVAVLTSLALVGTQGVLATHQPADKVVASGSKLAVVGP
                     GDEVPILTATLRSTNVSDLILGVSLECAILTQLTTGPNAVDGVDQALAQGKVLVWVEI
                     DGQIVPINSVGGESQPGDESDKVTFCDREYQRKVSDNEDPADGQDTEEDYIRTKAANA
                     FNWIHLNVGRGVHEIVVKATLTERVEGAAVAEALIGNRTLVVEPAKLANDATV"
     gene            complement(652..1326)
                     /locus_tag="VNU01_11570"
     CDS             complement(652..1326)
                     /locus_tag="VNU01_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH33297.1"
                     /translation="MTRRSYGQACPLAAALDRVGERWTALVIRDLASGPRRYSDLLRG
                     LPGIGTTLLAERLRSLQDEGLVAQRELPPPAASTVYELTTAGWELAAALAPLAAWGTR
                     FVATDDGARLRPEWLILAMAQGLSPADADGVRDVYEFHVDGTVFHVAVEDGRLSGAAA
                     PAPRPPDAVIRTDWTTLLALGSGALTPREALEQGRAAFDGDPAALARCGALLGRPATT
                     SRPAGA"
     gene            complement(1336..3429)
                     /locus_tag="VNU01_11575"
     CDS             complement(1336..3429)
                     /locus_tag="VNU01_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33298.1"
                     /translation="MPAAATREEPRLPTPRGWPFADAFPRTSGTGRLHQGTVQWTDFL
                     YDDRGARGVPVRSAITPLAPTRGTYEYLAAEAAGNGADIFRAAIGLDAEATYWRVDWN
                     TLVDPAVPIAQFALDVDGSASTGAADWPAAAGVRSPGIERALLLSAKGAWLVDAATGA
                     RRALGAPAVDLAARSFVLRVPRATLPVAGRWTVRLAAGVADASGEAFAPVPPSHGARP
                     GGTAVYNVAFRTHAQEPPLTGSPRSPLVANYWQEVAQATALADGDVTPFALELDWAAL
                     AAGATTAEESPAGWSTRWYVSSIEPGHGVVADTGGRQDLRPNYLGRVQPYAVYVPSSY
                     RPDRPAPLTWLLHSAFQPHTAFAVVTPRFAEAACERRGSICATPLGRGHDGWYLDEAE
                     LDFWEVWNRLAAAYALDPDRTVLAGFSMGGYAAYRLGFAHPHLFSGGVAIGGTPTCGE
                     RVAPGVERPAGPGRCATDGDTTALVGNARWLPFHIAHGTADELLPFTGTAAHVAAFDR
                     LRYRYRFDHYAGQTHVPWYQQDRWDDIAAAMPSGPRTRTVDRVTLRWRPHLARPEWGL
                     GVTGAYWARDLRPRTVTAGGTARLDARALARPLPAMLPTRTFRLATPDSTTPALVTEV
                     TWTAGATPAAEARLALDLRNVAHVRVALDEAGFGDGPVTVSTRSDGPATVTLTHGART
                     YTLTVAAGDQTHVVR"
     gene            3686..4879
                     /locus_tag="VNU01_11580"
     CDS             3686..4879
                     /locus_tag="VNU01_11580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWH33299.1"
                     /translation="MREAVIVDAVRTPVAKGKPGGALYETHPVDATAHVLRALVERNG
                     IDPAQIDDVLTGVVGQAGDQALNLSRNAALAAGYPEQVPAVTIDRQCGSSQQAATFGA
                     QGVMTGSYDLVVAAGVESMSRVPMGSALMGRNPFGEAFGERYPGGLIPQGVAAERIAL
                     KWGLSREQLDELALRSHQLAAAAEDAGAFASEIVPIEVGDGQGGTRLFDRDEGIRRET
                     TLEALAGLKPAFFDTAWEERFPGLPWVVTAGNSSPINDGASAVLIASPEKARELGLRP
                     RARFHTFAVTGDDPLMMLTAVIPVTFKAIERSGLTLDDIDLFEVNEAFASVVGAWQAE
                     TGVPWDKVNVNGGAMAIGHPLGSSGTRLLGTLVNALEQRGARYGMQVMCEAGGLANAT
                     IVERL"
     gene            complement(4890..5363)
                     /locus_tag="VNU01_11585"
     CDS             complement(4890..5363)
                     /locus_tag="VNU01_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH33300.1"
                     /translation="MKRTSFEHWQCSIAQTVEVVGDWWSILILRDVFWGLHRFDELLE
                     DLGIARNILSDRLSNLVAKGVLEKVPYQQAPVRHEYRLTKKGRDLWPVLMMMAQWGDR
                     WSLEGTHPIEFEHTACGERVHAVLSCDHCGERLGGQELRMHFNVSREAASGKRAS"
     gene            complement(5429..6163)
                     /locus_tag="VNU01_11590"
     CDS             complement(5429..6163)
                     /locus_tag="VNU01_11590"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWH33301.1"
                     /translation="MAGTTDYSDLRALYINCTLKRSPEPSHTQTLIDRSAAIMRAQGV
                     RVDMIRAVDHDIPPGVYPDLREHGWPSDEWPSLYERVHAADILVLATPIWLGDKSSVC
                     TKVIERLYGNSAQLNDAGQYAYYGRTGGCLVTGNEDGIKHCAMNILYSLQHLGYVIPP
                     QADAGWIGEAGPGPSYGDDGTGLDNEFTNRNTTFMTWNLLHVARWLADAGGFPVGGNQ
                     RTAWDAGERFGFVTNIDEHASDAEGR"
     gene            complement(6195..7016)
                     /locus_tag="VNU01_11595"
     CDS             complement(6195..7016)
                     /locus_tag="VNU01_11595"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH33302.1"
                     /translation="MTTTSAAASSEPETFGDLLREWRRRRRLSQLELSLSAGVSARHL
                     SFLETGRARPSREMALALAERLDVPLRERNLLLAAAGFAPAYPRRPLEAPEMGAVREA
                     IDLVLRNHDPYPAIAVDRYWNILAMNAGAAVFAQDLSPELLGPPANVYRITFHPKGWA
                     TRVVNYADLATDMIGRLRHDVAVSGDPELAALLEEVQAYGTLPPAPPQPPPSSVVLPV
                     RFRTPAGELSLFTVIATFGTPVDVTVSELAIETFFPADEATAERLRALAAGGGGA"
     gene            7143..7523
                     /locus_tag="VNU01_11600"
     CDS             7143..7523
                     /locus_tag="VNU01_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33303.1"
                     /translation="MRTRPWIPAFLRFDAALNAAGVLVIVVAAAPLAGALGLSARRPL
                     YAVAALLAVNGVELVLTARAPRPGMLTALALIDAAFVVLVLGYAATAQGAETWARWAL
                     VAVADATIVTAAAKLVGRRAPALA"
     gene            complement(7576..>8050)
                     /locus_tag="VNU01_11605"
     CDS             complement(7576..>8050)
                     /locus_tag="VNU01_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33304.1"
                     /translation="RLAGSWLVLFGAGYAALELAVVAGAVLGGVPPASAVAAGLPTDP
                     VLVLDLAFVLPGMAACGLLVRRRRAAALLLAVPALAFSTAMGVAVLNMLVVTGLRLPP
                     FPTVVVAGFVLITAGHATVLARVLRAMHPGVRLAGAVEPRRPAREDRPAATVRVS"
BASE COUNT         1100 a         2912 c         3041 g          997 t
ORIGIN      
        1 gggatggtcc tgaccgtggt ggtcgccgtg ctgacgagcc tggcgctggt gggcacccag
       61 ggagtgctgg cgacgcatca gccggcggac aaggtggtgg cgtcgggctc caagctcgcc
      121 gtcgtcggcc caggcgacga ggtgccgatc ctcaccgcca cgctgcgcag caccaacgtg
      181 agcgacctca tcctgggcgt gagcctggaa tgcgcgatcc tcacccagct caccaccggg
      241 ccgaacgccg tcgacggcgt cgaccaggcc ctggcccagg gcaaggtcct ggtctgggtg
      301 gagatcgacg ggcagatcgt gcccatcaac agcgtgggcg gcgagtcgca gccaggcgac
      361 gagtccgaca aggtgacgtt ctgcgaccgc gagtaccagc ggaaggtctc cgacaacgag
      421 gacccggcgg acggccaaga caccgaggag gactacatcc gcaccaaggc ggccaacgcg
      481 ttcaactgga tccacttgaa cgtgggccgc ggggtgcacg agatcgtggt gaaggcgacg
      541 ctcaccgaac gtgtcgaggg cgccgccgtc gcggaggcgc tgatcggcaa ccgcaccctg
      601 gtggtggagc cggccaagct cgccaacgac gcgacggtct gagcagccgc gtcaggcgcc
      661 ggccggcctc gacgtggtgg ccggccggcc cagcagcgcc ccgcagcgcg cgagcgcggc
      721 ggggtcgccg tcgaacgccg cgcgcccctg ctccagggcc tcgcgcggcg tcagcgcgcc
      781 ggagcccagc gccaacagcg tcgtccagtc cgtgcggatc acggcgtccg gcggccgcgg
      841 ggcgggcgcc gccgccccgg acaggcggcc gtcctccacg gcgacatgga acacggtgcc
      901 gtcgacgtgg aactcgtaga catcccgcac accgtcggcg tcggcgggcg agagcccctg
      961 cgccatcgcg aggatcagcc attcggggcg caggcgcgcc ccgtcgtccg ttgccacgaa
     1021 gcgggttccc caggccgcga gtggcgcgag cgcggccgcg agctcccatc ccgccgtcgt
     1081 gagctcgtac accgtggacg ccgccggggg cggcagctcg cgctgggcca ccaggccctc
     1141 gtcctgaagc gaccgcaggc gctcggccag cagcgtcgtg ccgatgcccg gcaggccgcg
     1201 tagcagatcc gagtagcggc gcgggccgct ggcgaggtcg cggatcacca gggcggtcca
     1261 ccgctcgccc acccggtcca gcgcggcggc gagggggcag gcctgaccgt aggagcgccg
     1321 tgtcatggcg gcaccctacc ggaccacgtg cgtctgatcg cccgcggcaa ccgtcagcgt
     1381 gtacgtccgc gcgccgtgag tcagcgtcac ggtcgccggg ccgtcgctgc gcgtcgacac
     1441 ggtgaccggg ccgtcaccga agccggcctc gtccagggcc acgcggacgt gcgcgacgtt
     1501 gcgcaggtcc agcgccagac gggcctcggc ggcgggcgtc gcgcccgccg tccacgtcac
     1561 ctcggtgacc agcgccggtg tcgtgctgtc gggcgttgcg agccggaacg tccgcgtggg
     1621 cagcatggcc ggcagcggcc tggccagcgc gcgtgcgtcg agccgtgcgg ttccgccggc
     1681 ggtgacggtc cgcggacgca ggtcgcgggc ccagtacgcg ccggtcacgc ccaggcccca
     1741 ttccggccgg gccaggtgcg ggcgccagcg cagcgtcacg cggtcgaccg tgcgggtgcg
     1801 cgggccagat ggcatcgccg ccgcgatgtc gtcccagcgg tcctgctggt accacggcac
     1861 gtgcgtctgg ccggcgtagt ggtcgaagcg gtagcggtag cgcaggcggt cgaaggccgc
     1921 cacgtgcgcg gccgtgccgg tgaacggcag cagctcgtcg gcggtgccgt gcgcgatgtg
     1981 gaacggcagc cagcgggcgt tgcccaccag cgccgtggtg tcgccgtccg tggcgcagcg
     2041 gcccggtccg gccgggcgct cgacccccgg cgcgacgcgc tcgccgcagg tcggcgtccc
     2101 cccgatcgcc acgccgcccg agaacaggtg cggatgggcg aagcccagcc ggtacgccgc
     2161 gtagccgccc atggagaagc cggccagcac cgtgcggtcc gggtccaggg cgtaggccgc
     2221 cgccagccgg ttccacactt cccagaagtc aagctccgcc tcgtcgaggt accagccgtc
     2281 gtgaccgcgg cccagcgggg tcgcgcagat cgagccgcgg cgctcgcagg ccgcctcggc
     2341 aaagcgcggc gtcacgacgg cgaaggccgt gtgcggctgg aacgccgagt gcagcagcca
     2401 ggtcagggga gccgggcggt ccgggcggta ggaggaaggg acgtacaccg cgtacggctg
     2461 cacgcggccg agatagttgg gccgcaggtc ctggcggccg ccggtgtccg cgacgacccc
     2521 gtggccaggc tcgatggacg agacgtacca gcgcgtggac cagccggccg gtgactcctc
     2581 ggccgtcgtg gcgcccgccg ccagcgcggc ccagtccagt tccagcgcga agggcgtcac
     2641 gtcgccatcg gccagcgcgg tcgcctgcgc cacctcctgc cagtagttgg cgaccagcgg
     2701 cgagcgcggc gacccggtga gcggcggctc ctgggcatgc gtccggaacg cgacgttgta
     2761 gacggccgtc ccgcccggcc gggcgccgtg gctcgggggc acgggtgcga acgcctcacc
     2821 gctggcgtcg gcgacgcccg ccgccagccg cacggtccag cgccccgcga caggcagcgt
     2881 ggccctgggc acgcgcagga cgaacgagcg cgccgccagg tcgacggccg gcgcgccgag
     2941 cgcgcggcgc gcgccggtcg ccgcgtcgac cagccaggca cccttcgccg acagcagcag
     3001 ggcgcgctcg atgccggggg agcgcacgcc agcggcggcc ggccagtccg cggcgccggt
     3061 cgacgccgag ccgtcgacgt cgagggcgaa ctgggcgatc ggcaccgccg ggtccaccag
     3121 ggtgttccag tccacgcgcc agtacgtcgc ctcggcgtcc aggccgatcg ccgcgcggaa
     3181 gatgtccgcg ccgttgccgg ccgcctccgc tgccaggtac tcgtaggtgc cgcgcgtcgg
     3241 cgccaggggc gtgatcgcgg agcgcaccgg cacgcccctg gcgccccggt cgtcgtagag
     3301 gaagtccgtc cactgcacgg tgccctggtg gaggcgcccc gtcccggacg tgcgcgggaa
     3361 cgcgtccgcg aacggccatc cgcggggtgt gggcagccgc ggctcctcgc gcgtggcggc
     3421 ggcgggcatg tccgcgggca agcctgccag ggctgggcgg ctggcggagc ttggggccgg
     3481 cgcggcgacg ccgagcagca ggatcgcggc cagggcggtg gaggcgtgtc gccggggtgt
     3541 gcgcataact caagatacaa gagtgactac tacaaaatca agagtgaatg tgggaagtgg
     3601 acccatgcgc gttgcgaacg cgcgcgtccg ccagtaagtt gcgatatgaa accaacggag
     3661 gttggtcgcg gcgaaaggga aagccatgcg tgaggcagtg atcgtcgacg cggttcgcac
     3721 gccggtcgcg aagggaaagc ccggcggggc cctgtacgag acccaccccg tggacgcgac
     3781 ggcacacgtg ctgcgcgccc tggtggagcg caacggcatc gatccggccc agatcgacga
     3841 cgtgctgacc ggcgtggtgg gccaggccgg cgaccaggcg ctgaacctgt cgcgcaacgc
     3901 ggccttggcc gccggctacc ccgagcaggt cccggcggtc accatcgacc gccagtgcgg
     3961 ctcctcccag caggcggcga ccttcggcgc ccagggcgtc atgaccggct cctacgacct
     4021 cgtggtcgcg gccggcgtgg agtcgatgag ccgcgtgccg atgggctcgg cgctcatggg
     4081 ccgcaacccc ttcggcgagg cgttcggcga gcgctacccc ggcgggctca tcccgcaggg
     4141 tgtcgcggcg gagcgcatcg cgctcaagtg gggcctgagc cgcgagcagc tcgacgagct
     4201 cgcgctgcgc agccaccagc tcgccgccgc cgcggaggac gccggcgcgt tcgcgagcga
     4261 gatcgtgccg atcgaggtgg gtgacggcca gggcggcacg cgcctgttcg accgcgacga
     4321 gggcatccgg cgggagacca cgctggaggc cctggcgggg ctcaagcccg cgttcttcga
     4381 caccgcctgg gaggagcgct tccccgggct gccgtgggtg gtgacggcgg gcaactcctc
     4441 gccgatcaac gacggcgcct ccgcggtgct gatcgcctct cccgaaaagg cccgggagct
     4501 gggcctgcgg ccgcgggcgc gcttccacac gttcgccgtg accggcgacg acccgctgat
     4561 gatgctcacc gccgtcatcc ccgtgacctt caaggcgatc gagcgcagcg gcctcaccct
     4621 ggacgacatc gacctgttcg aggtcaacga ggcgttcgcc tccgtggtgg gcgcctggca
     4681 ggcggagacg ggcgtgccgt gggacaaggt caacgtcaac ggcggcgcca tggcgatcgg
     4741 gcacccgctg ggcagctccg gcacgcgcct gctcggcacg ctggtgaacg cgctggagca
     4801 gcgcggcgcg cgctacggca tgcaggtgat gtgcgaggcg ggcgggctgg ccaacgccac
     4861 catcgtcgag cgcctgtgag gcgggccggt caggaggcgc gcttgccgga ggcggcctcg
     4921 cgcgagacgt tgaagtgcat gcgcagctcc tggccgccca gccgctcgcc gcagtgatcg
     4981 caggacagca ccgcgtgcac gcgctcgccg caggcggtgt gctcgaactc gatggggtgc
     5041 gtgccctcca gcgaccagcg gtcgccccac tgcgccatca tcatcagcac cggccacagg
     5101 tcgcggccct tcttggtgag gcggtactcg tggcgcacgg gggcctgctg gtagggcacc
     5161 ttctccagca cgcccttggc gaccaggttg ctcaggcggt ccgacaggat gttgcgggcg
     5221 atgcccaggt cctccagcag ctcgtcgaag cggtgcagcc cccagaacac gtcgcgcagg
     5281 atgaggatcg accaccagtc gcccaccacc tccacggtct gggcgatgga gcactgccag
     5341 tgctcgaacg acgtgcgttt catgagggcc tccggggctg cggggcagcg ggtcggtagc
     5401 ctcatgctac ccagcaggcg ccccgcggtc agcggccctc ggcgtcgctg gcgtgctcgt
     5461 cgatgttcgt cacgaagccg aagcgctcgc cggcgtccca cgccgtgcgc tggttgccgc
     5521 ccacggggaa gccgccggcg tcggcgagcc agcgcgccac gtgcagcagg ttccacgtca
     5581 tgaacgtggt gttgcggttg gtgaactcgt tgtccaggcc ggtgccgtcg tcgccgtagg
     5641 acggcccggg cccggcctcg ccgatccagc cggcgtcggc ctgcggcggg atcacgtagc
     5701 ccaggtgctg cagcgagtac aggatgttca tggcgcagtg cttgatgccg tcctcgttgc
     5761 cggtcaccag gcagccgccc gtgcggccgt agtaggcgta ctggccggcg tcgttgagct
     5821 gcgcggagtt gccgtacagg cgctcgatga ccttggtgca caccgacgac ttgtcgccca
     5881 gccagatggg tgtggccagc accaggatgt cggccgcgtg cacgcgctcg tacagcgacg
     5941 gccactcgtc gctgggccag ccgtgctcgc gcaggtccgg gtacaccccc ggggggatgt
     6001 cgtggtccac cgcgcggatc atgtccaccc gcacgccctg cgcgcgcatg atcgccgcgg
     6061 accggtcgat cagggtctgc gtgtgcgagg gctccggcga gcgcttcagc gtgcagttga
     6121 tgtacagcgc gcgcaggtcc gagtagtccg tggtgccggc catgtccgcc tcctgggggt
     6181 ggtgccggcc gagcctaggc cccgccgccg ccggcggcca gggcgcgcag gcgctcggcc
     6241 gtcgcctcgt cggccgggaa gaacgtctcg atggccagct ccgacacggt gacgtccacc
     6301 ggcgtgccga aggtcgcgat cacggtgaac agcgacagct cgccggcggg cgtgcggaag
     6361 cgcaccggca gcaccaccga gctcggcggc ggctgtggcg gcgccggggg gagggtgccg
     6421 taggcctgca cctcctccag cagtgccgcc agctccgggt cgccggacac cgcgacgtcg
     6481 tggcgcaggc ggccgatcat gtcggtggcc aggtcggcgt agttgacgac gcgcgtggcc
     6541 cagcccttcg ggtggaacgt gatgcggtag acgttggccg gcggccccag cagctccggg
     6601 gaaaggtcct gggcgaacac cgcggcgccg gcgttcatcg cgaggatgtt ccagtagcgg
     6661 tccacggcta tggcgggata ggggtcgtgg ttgcgcagca ccaggtcgat cgcctcgcgc
     6721 accgcgccca tctccggcgc ctccagcggc cggcgcgggt aggcgggcgc gaagccggcg
     6781 gcggcgagca gcaggttgcg ctcgcgcagg ggcacgtcca ggcgctcggc gagcgccagc
     6841 gccatctcgc ggctgggccg ggcgcgcccc gtctccagga agctgaggtg ccgcgccgac
     6901 acgccggccg acagcgacag ctccagctgg ctgagccggc ggcgccgccg ccactcgcgc
     6961 agcaggtccc cgaacgtctc cggctcgctg gaggccgccg cggatgtcgt cgtcatgggc
     7021 cgcacgctac cgccgcgggg gaatcgcgga cattacctgc gaggtaatcg acagccgcct
     7081 tgtgacgcgc cacggtgccg ccatggccac ccgccagatc gactgacacg aaaggcatcg
     7141 tcatgcggac ccgcccctgg atccccgcgt tcctccgttt cgacgccgcg ctcaacgccg
     7201 ccggcgtgct ggtgatcgtc gtcgccgcgg cgccgctggc cggcgcgctc ggtctatcgg
     7261 cgcgccggcc gctgtacgcg gtggccgcgc tgctcgcggt caacggcgtc gagctggtgc
     7321 tgacggcccg cgccccgcgc cccgggatgc tgaccgcgct cgccctgatc gacgccgcgt
     7381 tcgtggtgct ggtgctcggc tacgccgcca ccgcgcaggg cgcggagacg tgggcgcgct
     7441 gggcgctggt cgccgtggcc gacgcgacga tcgtcaccgc cgccgcgaag ctggtgggac
     7501 gcagggcccc ggcgctggct tgaccagggt caggcgcgcc gcggcgcggc gtcagcaccg
     7561 acatctcggc tcctttcacg acacgcgcac ggttgcggcc ggccggtcct cgcgggcagg
     7621 cctgcgcggc tcgacggcgc cggccagccg cacgcccggg tgcatggcgc gcaggacgcg
     7681 tgccagcacg gtcgcgtgcc cggccgtgat caggacgaac ccggccacga ccaccgtcgg
     7741 aaacggcggc agccgcaggc cggtcaccac gagcatgttc agcaccgcca cgcccatggc
     7801 ggtgctgaac gcgagcgccg gcacggcgag cagtagcgcc gcggccctcc ggcggcggac
     7861 cagcaggccg caggccgcca tcccgggcag cacgaaggcg aggtcgagca cgagcaccgg
     7921 gtccgtgggc agcccggcgg ccaccgccga cgcgggcggc acgccgccca gcaccgcccc
     7981 ggccaccacg gccagctcca gggccgcgta cccggcgccg aagagcacca gccacgatcc
     8041 cgccagccgc
//
LOCUS       DATMKC010000399         9970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_4602, whole genome shotgun sequence.
ACCESSION   DATMKC010000399 DATMKC010000000
VERSION     DATMKC010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4602"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VNU01_11610"
     CDS             complement(<1..831)
                     /locus_tag="VNU01_11610"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor RsbA family regulatory
                     protein"
                     /protein_id="HWH33305.1"
                     /translation="MSTLARTPGHRHEALLYRGEDEFVGRVAAFLREGVEAGEEVLVA
                     VSPPKFALLTEALGDAAGGVGYLDMDEVGRNPGRLISAWHDWAAARPGRALRGVGEPV
                     TMARTPAETAECHASEALWNVAFAGHDLWVLCPYDTAALAPDRLASVERTHPAVIADG
                     VATPSARYSQPWTSVLTEPLTEPGQVHTTLAFGSLQRVRDAVADAARRAGAGRGRAED
                     LVIAVNEVACNSVRHGGGSGTLRVWEADGAVVCEVRDQGRLKDPLVGRLRPEASRVSG
                     R"
     gene            complement(828..1652)
                     /locus_tag="VNU01_11615"
     CDS             complement(828..1652)
                     /locus_tag="VNU01_11615"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HWH33306.1"
                     /translation="MTASLVAGQSSRHVCAVTRTDAARARITAGWIREGLELGERVVW
                     VEPPGGPVLGWLDAGGFDWRGPLASGALRLAPPEDVLLLDSPADIPARLADARALTAQ
                     ALRDGHSGLRVGADTAVALTVMAGVDAQLRFEAAWEELTRVERLSLLCLYDPAVHGEH
                     LDRGIALHPREFTDGLAVAAAAAGTVEIGGDLDLSNAAMVGAFLRAADLDAGPGDRTL
                     DLRACAFVDIAGADRLVAFARAAAPRRVRVLGPPPTLRRILELTGGGELELVGGPR"
     gene            complement(1734..4598)
                     /locus_tag="VNU01_11620"
     CDS             complement(1734..4598)
                     /locus_tag="VNU01_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HWH33307.1"
                     /translation="MGEQSIPIVGRERELEALDAVLSTVAAGRAQAVFLEGEPGIGKS
                     RLLDETVRRAQERGFAILHGRAEELTRTTPFAAVGEALGWNAAGQDPVSARIAALLGA
                     DAPQLAAPGAVRDVGGQVIELVLELLHARTGDGPVLLALDDLHWADPGTLRTVRAIAR
                     RLTSLPVALVATTRPTQGTGDLQRLVDTCADEGAARLILGPLDAQALAALAGLVLGAP
                     AGTSVTRGLAGTAGSPMFALELLKAWQREGAITVSDGAAETAGEALPETLRLAVVRRL
                     YVLEPATLEALRLAAVLGSTFTLAGLCAMAERSASALLGCVSEAMEAGLIAEDHDQLR
                     FRHDVIREALYEELPRTVRAALHRQAAHTLSQAGAPVVQVARHLALGAAPGDAEAIRA
                     LRQAAAHTVLLSPRLAVEFLDSARGLLQPGDAAHDELGAELILPLHLIGRTGDAERLA
                     GDILSRRPPPRVRYLLQRGLAYALTVTGRQREAVAQYEAITAEPPAAGWTASPHAAHA
                     IDLANLGIGLALLNEVERAREPIGRALAMAAHAHDDYAMSIALLAETTVADVEGRVHD
                     ALDSARRNVSLVTSQPAAMHGNLWAHCQLGFALLAADLLGEAAEVLRTGLRLVEEQGR
                     VAQLSPYHIGLGSGSALAGEWDDAIAHLEAGISAGEEHVFTTLPASGALSWLHLHRGE
                     FAAARQAVPPRDGDAPLGGFGQDWVAVAEAALLEAEGRAQPALARLRATWERQRDGGA
                     LFGFGTWRLSGTMLVRLARQTGDDAFALAVAEDAADGARRAGGVTSADAAALHCRGLA
                     DGDLTALMDAVAAYRQGPHALLAGLACEDAGTALVKAGRQKDGRQALRQALDVYRDLR
                     ARWDLDRLDATCRALGIRQVRATRRPAATTGWDSLTATEQRVTGLVAEGLSNAEVAQR
                     LYLSRYTVETHLKHIFAKLGVGSRVELAVQAARRDASPTA"
     gene            4803..5432
                     /locus_tag="VNU01_11625"
     CDS             4803..5432
                     /locus_tag="VNU01_11625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH33308.1"
                     /translation="MPAADEAQPVTKVRVRKATASDVAGLAAVMGRAFQEDPVLAWGM
                     PDEAGRRANGARYFELVARLHYLPLGETYVTEDLQAAALWAPPGQWKASPMTGVRILP
                     FLVRATKFGRALKILKLLEHKHAELREPHFHLFAVGTDPLRQGRGYGGALLRHVLDRC
                     DEQGMPAYLEATTERNRALYLRHGFRVMEELRLPEGGPPFWRMWRGSRS"
     gene            complement(5465..6013)
                     /locus_tag="VNU01_11630"
     CDS             complement(5465..6013)
                     /locus_tag="VNU01_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33309.1"
                     /translation="MTMWRVLAVGVCLFATACGGGQEPTGGIAEAAQRQAAYDDFADC
                     LRDQGANVGHPVLSPDGSVQLPPGDAGALSSVEELSRAAARCQPILSARGLPPPGPVT
                     LSDHELADLRRRSKAFAGCLRTEGIDWPDPEWDGGAIVNWDPEAVGVDLDDPDVQRIG
                     EDCSARTGFDPMLAHSVDDEQT"
     gene            complement(6285..6788)
                     /locus_tag="VNU01_11635"
     CDS             complement(6285..6788)
                     /locus_tag="VNU01_11635"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWH33310.1"
                     /translation="MPANATINVGDTVVHPHHGVGTVTALDTVDLGAGPVPYVTISVD
                     GGLTLKVPAATLSDHGIREPVTEKRAEEILAVLTQPAAEDPGHAVRRRRDADKLASGK
                     LVECAEVVRDTAAVIAAHAKGGADADRNLLSNAKTQLAQELAVALGSTPEEAMARVEA
                     QLKSRND"
     gene            complement(6922..7509)
                     /locus_tag="VNU01_11640"
     CDS             complement(6922..7509)
                     /locus_tag="VNU01_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33311.1"
                     /translation="MVKEHAEAAGWEVLVRQTQNPASDAQWLAQNQIKVAFPMVTPTV
                     FLQIVRSPGGSIDQWAGVGITMGLNTVAKTACTANPDFDGAMFFSPVPGLNVIDQVDP
                     EFRKAGGVDDIELLLWGVAKSVHEVFKKMPADDLSRQRFMSTLEHNVLDSRLYPALHH
                     SPSNHMNAQGVHLLFANCDTQQYETPPDGLFRTSF"
     gene            complement(7503..7922)
                     /locus_tag="VNU01_11645"
     CDS             complement(7503..7922)
                     /locus_tag="VNU01_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH33312.1"
                     /translation="MPQTSFATGKDLYWRHIGKVHGRTVEVFLVNDEYNPSRATSVCN
                     DLIQREKVYVLIGGAGTDQISACARTAATQGVPYLSAGVDETQVRSLKNYFALSMTYI
                     QQAPMLIQWVKKHSPPPNGKFGILRDRTPGFNAVVAW"
     gene            complement(7937..8329)
                     /locus_tag="VNU01_11650"
     CDS             complement(7937..8329)
                     /locus_tag="VNU01_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33313.1"
                     /translation="MGTVALRRLALVLSMAVLAVACGQKDGAHDVALGPVDPAAAFDE
                     GANPADGSTPGDELTDGVTPQDGGDQPDTGTPPGDDPDANERSQDTGDRSRPRTTPPR
                     SPRRSRAARRATPPASPTPPSRSGCTRR"
     gene            complement(8460..>9970)
                     /locus_tag="VNU01_11655"
     CDS             complement(8460..>9970)
                     /locus_tag="VNU01_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HWH33314.1"
                     /translation="SGGAAPVAIFTNGTEGDLIPRADECNQHACADRLGLRVAAAMRQ
                     AWDAAGANLAGDLPVAGAARRVRYDGQELEPGKRVARRAWFGLPFVGGAANGPHFLYG
                     FVEGRRRPRALAGPVHGRKLLAMPAPWSPAADVTVLRVGDRLLLGVPGEPSLEAGRRM
                     CAAALEAGGGAAGTAVAEAMVVGLAQGYRGYFTTPEEYDQQHYEGGHTVFGKHTSLLV
                     QQAHAELAAELCTAPLPLRSQSRPTVATIDSAPAAAAPVSIGGAAPRILREPPDEVMR
                     FDTMSWSWSAPPLGRARPVDAPYLVLERLAEDGAAEAVEDDLGTGFVWEQRGGRVTGW
                     YEVPGDLPPGRYRFAVRTVNASAVTRAFTVGASAGLLLLGVAAEQARLVFRAQNPPPD
                     PLASLRARERSPSGGVLRFCAGGRELEAVWDEAAQGWAAEVGDVPAEVTVPEGGLRDA
                     AGNRSGGAVALTVGQRRPLQWPPPMGPGGGRCPAPFGRGPAGKPAAWRPTGG"
BASE COUNT         1229 a         3818 c         3643 g         1280 t
ORIGIN      
        1 gcggccgctg acgcgtgacg cctccggccg cagccggccg accagcgggt ccttgaggcg
       61 gccctggtcg cgcacctcgc acaccacggc gccgtcggcc tcccataccc gcagggtgcc
      121 ggacccgccg ccgtggcgga cgctgttgca cgccacctcg ttcaccgcga tgaccaggtc
      181 ctcggcgcgc ccgcggccgg cgcccgcccg tcgcgccgcg tcggcgactg cgtcgcgcac
      241 gcgctgcagc gagccgaacg ccagcgtggt gtgcacctgc ccgggctccg tcagcggctc
      301 ggtgagcacg gacgtccagg gctgcgagta acgggcgctg ggcgtggcga cgccgtcggc
      361 aatcaccgcc gggtgcgtgc gctcgacgct ggcgagccgg tccggcgcca gtgccgccgt
      421 gtcgtagggg cacagcaccc acaggtcgtg gccggcgaac gccacgttcc acagcgcctc
      481 ggaggcgtgg cactcggcgg tctccgccgg ggtgcgcgcc atcgtcaccg gctcgcccac
      541 gccgcgcagc gcgcggccgg ggcgggccgc cgcccagtcg tgccaggcgg agatcagccg
      601 gcccgggttg cggcctacct cgtccatatc caggtagccg acgccgccgg cggcgtcgcc
      661 gagcgcctcg gtgagcagcg cgaacttggg cgggctcacc gccacgagca cttcctcccc
      721 ggcctcgacg ccctcacgga ggaacgcggc gacgcggccg acgaactcgt cctcaccgcg
      781 gtacagcagc gcctcgtgac ggtgaccggg ggtgcgcgcg agcgtgctca tcggggaccg
      841 ccgaccaact cgagctcacc gccgccggtg agctccagga tgcgcctcag agtgggcggc
      901 ggtcccagga cgcgcacgcg tcgcggcgcc gcggcgcggg caaaggccac gagccggtcg
      961 gcccccgcga tgtcgacgaa cgcgcaggcc cgcaggtcca gcgtgcggtc gcccgggccg
     1021 gcgtcgaggt cggcggcgcg caggaacgcg cccaccatgg cggcgttgga caggtccagg
     1081 tcgccgccga tctccacggt gccggccgcg gccgcggcca ccgccaggcc gtcggtgaac
     1141 tcgcgcgggt gcagcgcgat gccgcggtcc agatgctcgc cgtgcacggc cgggtcgtac
     1201 aggcacagca gcgacaggcg ctccacgcgc gtgagctcct cccacgcggc ctcgaaccgc
     1261 agctgggcgt ccacgccggc catgacggtc agcgccactg cggtgtccgc gccgacgcgc
     1321 aggccgctgt ggccgtcccg gagcgcctgc gccgtcagcg cacgggcgtc ggccaggcgc
     1381 gccgggatgt cggccggcga gtccagcagc agcacgtcct cgggcggcgc cagccgcagg
     1441 gcgcctgacg ccaacgggcc gcgccagtcg aagccgcccg cgtccagcca gcccaggacc
     1501 gggccgccgg gaggctccac ccacaccacg cgctcaccca gttccaggcc ctcgcggatc
     1561 cacccggcgg tgatccgcgc gcgggcggcg tcggtgcgcg tcacggcgca cacatgccgg
     1621 ctcgattgcc ccgcgacgag ggaggcggtc acacgcggac ctttccgggc gcctgcccga
     1681 cggatggcca gtgaggcgat cctaggccgt ctccgccgga tgtgggcgaa gcttcaggcc
     1741 gtgggggagg cgtcccgcct ggcggcctgc accgccagct cgacccttga gcccacgccg
     1801 agcttggcga agatgtgctt gaggtgggtc tccaccgtgt agcgcgacag gtacaggcgc
     1861 tgggccacct cggcattgga cagcccttcg gcgaccaagc cggtgacccg ctgctcggtg
     1921 gccgtcaggc tgtcccagcc ggtcgtcgct gcggggcgcc tggtcgcgcg gacctggcgg
     1981 atgcccagtg ccctgcacgt ggcgtccagc cggtcgagat cccaccgcgc gcggaggtcc
     2041 cggtagacgt cgagcgcctg ccggagcgcc tggcgcccgt ccttctggcg ccccgccttg
     2101 accagcgcgg tcccggcgtc ctcacaggcc aggcccgcca gcagcgcgtg cggcccctgg
     2161 cggtaggccg ccacggcatc catcagggcg gtgaggtcgc cgtcggccag gccgcggcag
     2221 tgcagggccg cggcgtcggc tgacgtgacg ccgccggcgc gccgggcgcc gtcggcggcg
     2281 tcctcggcga ccgcaagcgc gaacgcgtcg tcgccggtct ggcgcgccag cctgaccagc
     2341 atggtcccgg acagccgcca cgtgccgaat ccgaacaggg cgccgccgtc gcgctggcgt
     2401 tcccacgtcg cgcggaggcg cgccagcgcc ggctgcgcgc gcccctccgc ctccagcagt
     2461 gccgcctcgg cgaccgcgac ccagtcctgg ccgaatccgc ccaggggcgc gtcgccgtcc
     2521 cgcggtggca ccgcctggcg tgccgccgcg aactcgcccc ggtgcaggtg aagccacgac
     2581 agcgcgccgg aggcgggcag cgtggtgaag acgtgctcct cgccggcgct gatcccggct
     2641 tccaggtggg cgatggcgtc gtcccactcg ccggcgagcg cgctgccgct gcccaagccg
     2701 atgtggtacg gcgacagctg cgcgacgcgc ccctgctcct cgaccaggcg caacccggtg
     2761 cgcaggacct cggccgcctc gcccagcagg tcggccgcca gcagcgcgaa accgagctgg
     2821 cagtgcgccc acaagttgcc gtgcatggct gccggctggg atgtgaccag cgacacgttg
     2881 cggcgcgcgc tgtcgagggc gtcgtgcacg cggccctcga cgtcggccac cgtggtctcc
     2941 gccaggagcg cgatggacat cgcgtagtcg tcgtgcgcgt gcgcggccat ggccagcgcc
     3001 cgtccgatcg gctccctggc gcgctcgacc tcgttgagca gcgccaggcc gatgcccagg
     3061 ttcgccaggt cgatggcgtg cgcggcatgc ggcgacgccg tccacccggc cgccgggggc
     3121 tccgccgtga tggcctcgta ctgggcgacg gcctcccgct gccgcccggt gacggtgagc
     3181 gcgtaggcga ggccgcgctg caggaggtag cggacgcgcg gcggtggccg gcggctcagg
     3241 atgtcgccgg ccaggcgctc cgcgtcgccg gtgcgcccga tcaggtgcag cggcaggatc
     3301 agctcggcgc ccagctcgtc gtgggccgcg tcgcccggct gcagcaggcc gcgggcggag
     3361 tccaggaact cgaccgcgag ccgcggcgac agcagcaccg tgtgcgcggc ggcctgccgc
     3421 agcgcgcgta tggcctccgc gtcaccgggc gcggcgccga gcgcaaggtg ccgggcgact
     3481 tggacgaccg gggctcccgc ctgcgacagc gtgtgcgcgg cctgccggtg cagcgccgcc
     3541 cgcacggtcc gcggcagttc ctcgtagagg gcctcgcgga tcacgtcgtg gcggaagcgg
     3601 agctggtcgt ggtcctcggc gatcagtccc gcctccatcg cttccgacac gcagcccagc
     3661 agcgcggagg cgctccgctc ggccatcgcg cacagccccg ccagcgtgaa cgtcgagccg
     3721 agcaccgcgg ccagccgcag cgcttcgagc gtggccggct ccaggacgta caggcgccgc
     3781 acgaccgcga ggcgaagcgt ctccggcagc gcctcaccgg cggtctcggc cgcgccgtcg
     3841 ctgacggtga tggcgccttc ccgctgccac gccttgagca gctcgagcgc gaacatcggg
     3901 ctgcccgccg tgccggccag gccccgcgtg accgacgtgc cggcaggcgc gccgagcacc
     3961 aggccggcca gcgccgccag cgcctgcgcg tcgagcgggc cgaggatcag ccgcgccgca
     4021 ccctcgtcgg cgcaggtgtc gaccagccgc tgcaagtctc cggtgccctg cgtggggcgc
     4081 gtcgtggcga cgagcgcgac cggaaggctc gtcaggcggc gcgcgatggc gcgcacggtg
     4141 cgcaacgtgc cggggtccgc ccagtgcagg tcgtccaggg ccaggagcac gggaccgtcg
     4201 ccggtccgtg cgtgcaacag ctccaggacc agttcgatca cctggccgcc cacgtcgcgc
     4261 acggcgccgg gcgccgccag ctggggcgcg tcggcgccca acagggcggc gatgcgcgcc
     4321 gacaccggat cctggccggc ggcgttccac ccgagcgcct cgccgacggc ggcgaacggc
     4381 gtcgtgcggg tcagttcctc cgcgcggccg tgcaggatcg cgaagcctcg ctcctgcgcc
     4441 cgccgcaccg tctcgtcgag cagacggctc ttgccgatcc ccggctcgcc ctccaggaag
     4501 accgcctgtg cgcggccggc cgccaccgtg gacagcaccg cgtccagcgc ttccagctcg
     4561 cgttcgcggc cgacgatggg gatcgactgc tcgcccaacg gctcgccttc cgacggctca
     4621 gtccggttcg ccggatcccc gccggacccg caccacagcg tagccattcc ggcgtcgtgg
     4681 ccgggcggcc ccacgaatac ctgagttcag ggatgtgggg ggtttgtggg ggtgccaggc
     4741 tcgacacgca ccaccggaag gggccaacgc cccgacatgg atcctgaaga aagggtgggg
     4801 acatgcccgc agcagacgag gcgcagccgg tcaccaaggt ccgggtccgc aaggcgaccg
     4861 catccgacgt cgcggggctg gcggccgtga tggggcgggc gttccaggag gaccccgtgc
     4921 tggcctgggg gatgcccgac gaggccggcc gccgtgcgaa cggcgcccgt tacttcgaac
     4981 tggtcgcacg cctgcactac ctgcccctgg gtgagaccta cgtcaccgag gacctccagg
     5041 cggcggcgct gtgggcgccc ccggggcaat ggaaggcctc gcccatgacc ggcgtccgga
     5101 tcctgccgtt cctcgtccgg gccacgaagt tcgggcgcgc gctgaagatc ctcaagctgc
     5161 tggagcacaa gcacgccgaa ctgcgggagc cgcactttca tctgttcgcc gtgggcaccg
     5221 acccgctgcg ccaggggagg ggctacggcg gcgcgctgtt gcgccacgtg ctcgaccgct
     5281 gcgacgagca ggggatgccc gcgtacctgg aggcgacgac cgagcgcaac cgcgcgctgt
     5341 acttgcggca cggcttccgc gtgatggaag aactgcgcct gccggagggc ggtccgccct
     5401 tctggcgcat gtggcgcggc agcaggtcgt gaggacgccg ctcacgcgct gcgggcacac
     5461 gccgctaggt ctgctcgtcg tcgaccgaat gggccagcat ggggtcgaag ccggtacgtg
     5521 ccgagcagtc ctcgccgatc cgctggacgt cagggtcgtc gaggtcgacc ccgaccgcct
     5581 cagggtccca gttcacgatc gcgccgccgt cccactccgg gtccggccag tcgatgccct
     5641 ctgtccgcag gcatccggcg aacgccttgg accgccggcg caggtcggcg agctcgtggt
     5701 cgctgagcgt gacggggccg ggcggcggca gacctcgggc cgagaggatc ggctggcacc
     5761 gcgccgcggc tcgtgacagc tcctcgaccg acgacagcgc gcccgcgtcc ccgggtggca
     5821 actgcacgct cccgtccggg gaaaggacag ggtgcccgac gttggcgccc tggtcgcgca
     5881 ggcagtcggc gaagtcgtcg tatgcggcct ggcgctgcgc ggcctcagct atcccgcctg
     5941 tcggctcttg cccaccgccg cacgccgtgg cgaacaggca cacgcccacc gccagcaccc
     6001 gccacatcgt catcccgcca cctcccgtct cgtccgaccg ggcgcttcga cgcgcgcgca
     6061 tcgcctcgcg ttccaccgtt cccgcaacga cctgccgaat ttagaagcct gcgaaggtgc
     6121 ccagggcagg gcggccgtcc agcaccggga tcatgaccga ggggctgacc agcgccggca
     6181 gcaggtggtc ggcgccgtgc cccggtgagc cgtggcgatg cgcgtagcgg tcgtcgcggg
     6241 gcagcagccg ctcaagcgcg tcttctttgc tgccggcccc cgggtcagtc gttccgggac
     6301 ttgagctgcg cctcgacgcg cgccatcgcc tcctcgggcg tggagccgag cgccaccgcc
     6361 agctcctggg ccagctgcgt cttggcgttc gacagcaggt tgcggtctgc gtcggcgccg
     6421 cccttcgcgt gcgccgcgat cacggcggcg gtgtcgcgca ccacctcggc gcactccacc
     6481 agcttgccgc tcgccagctt gtcggcgtca cgccggcggc gcaccgcatg ccccgggtct
     6541 tcggcggccg gctgcgtcag cacggccagg atctcctcgg cgcgcttctc cgtcaccggc
     6601 tcgcggatgc cgtggtccga cagcgtcgcg gcggggacct tcagggtgag gccgccgtcc
     6661 acggagatgg tgacgtaggg caccgggccc gcacccagat cgacggtgtc gagcgccgtg
     6721 acggtgccca cgccgtggtg agggtggacg acggtgtcgc ccacattgat ggttgcgttc
     6781 gccggcacga gtgctctcct gcgcaatgga tcgtctggac tccgaccata ccggcgcggg
     6841 gccgttcccg ccggtcggac gggccggttc gcctgtgcga accgcccgtc cgaccggggc
     6901 ctggggcgat ggaggactga gctagaagct ggtgcggaac agcccgtcgg gcggcgtctc
     6961 gtactgctgg gtgtcgcagt tggcgaacag caggtgcacc ccttgcgcgt tcatgtggtt
     7021 ggacggcgag tggtgcagcg ccggatacag gcggctgtcc agcacgttgt gctccagcgt
     7081 ggacatgaag cgctgccgcg acaggtcgtc cgccggcatc ttcttgaaca cctcgtgcac
     7141 gctcttggcc acgccccaca gcagcagctc gatgtcgtcg acgccgcccg ccttgcggaa
     7201 ctcgggatcc acctggtcga tgacgttcaa cccgggtacc ggcgagaaga acatcgcgcc
     7261 gtcgaagtcc gggttggcgg tgcacgccgt cttggccacg gtgttcagcc ccatggtgat
     7321 gcccacgccg gcccactggt cgatggaccc tccgggggag cgcacgatct ggaggaagac
     7381 cgtcggcgtc accatgggga acgccacctt gatctggttc tgcgccagcc attgcgcgtc
     7441 cgacgcgggg ttctgcgtct ggcggaccag cacctcccag ccggccgcct cggcgtgctc
     7501 cttcaccacg ccaccaccgc gttgaagccc ggggtgcggt cgcgcaggat gccgaacttg
     7561 ccgttcggcg ggggcgagtg cttcttcacc cactggatca gcatcggcgc ctgctggatg
     7621 taggtcatgg acagcgcgaa gtagttcttg aggctgcgca cctgcgtctc gtccacgccg
     7681 gcggacaggt acggcacgcc ctgcgtggcg gcggtgcgcg cgcacgcgct gatctggtcg
     7741 gtgcccgcgc cgccgatcag gacgtacacc ttctcgcgct ggatgaggtc gttgcacacc
     7801 gacgtcgcgc ggctggggtt gtactcgtcg ttgaccagga acacctcgac ggtgcggccg
     7861 tgcaccttgc cgatgtggcg ccagtacagg tccttgccgg tggcgaagga cgtctgcggc
     7921 accggggcgg cgccggtcag cggcgcgtgc agcccgatct tgatggtggt gtcggagatg
     7981 ccggtggtgt cgcccggcgc gctgcgcggc tgcgccgagg gctgcgcggt ggggtcgtgc
     8041 gcggacggct gcggtccccg gtgtcctggg agcgctcgtt ggcgtccggg tcgtcgcctg
     8101 gcggcgtccc ggtgtccggc tggtccccgc cgtcctgcgg ggtcacgccg tcggtgagct
     8161 cgtcgccggg ggtggagccg tcggccgggt tggcgccctc gtcgaacgcc gcggccgggt
     8221 ccaccggccc cagcgcgacg tcgtgcgcgc cgtccttctg cccgcacgcc accgccagca
     8281 ccgccatcga cagcaccagc gccagccggc gcagcgccac cgtccccatg tcccgccctc
     8341 tcggtcatgc cggcgcgatt cccgccacgc cggcgcccgc acgcctcact gagcaagacg
     8401 ctcgcccgcc cgatccggtt ccccgcgacc gcaccgcagg cgcggacgct agcaggccgt
     8461 caccctccgg tgggccgcca ggccgcgggc ttgcccgccg ggccgcggcc gaacggcgcg
     8521 gggcagcgcc cgccgccggg ccccatgggc ggcggccact ggagcggccg ccgctgaccg
     8581 accgtgagcg cgaccgcgcc accggagcgg tttcccgccg cgtcgcgcag cccgccttcc
     8641 ggcaccgtca cctcggcggg cacgtcaccg acctcggccg cccagccctg cgccgcctcg
     8701 tcccacaccg cttcgagctc gcgcccgccg gcgcagaagc gcaggacgcc gccggacggc
     8761 gagcgctccc tggcgcgcag cgacgccagc gggtccggcg gcgggttctg cgcgcggaac
     8821 accaggcggg cctgctccgc cgccacgccg agcagcagca agccggccga ggcaccgacg
     8881 gtgaacgccc gcgtgaccgc cgacgcgttc acggtgcgca ccgcgaagcg gtagcggccc
     8941 ggcggcaggt cgccgggcac ctcgtaccag ccggtgacgc ggccgccgcg ctgctcccac
     9001 acgaagccgg tgcccaggtc gtcctccacg gcctcggcgg cgccgtcctc cgccaggcgc
     9061 tccagcacca ggtagggggc gtcgacgggg cgggcgcggc ccagcggcgg cgcggaccac
     9121 gaccacgaca tcgtgtcgaa gcgcatgacc tcgtcgggcg gctcgcgcag gatgcgcggc
     9181 gcggcaccgc cgatgctcac gggcgccgcc gccgccggtg cggagtcgat tgtggccact
     9241 gtcggccggg attgactccg cagcgggagg ggggcggtgc agagctcggc ggccagctcg
     9301 gcgtgggcct gctgcaccag caggctggtg tgcttgccga acaccgtgtg cccgccctcg
     9361 tagtgctgct ggtcgtactc ctccggcgtg gtgaagtagc cgcggtagcc ctgcgccagg
     9421 cccaccacca tcgcctcggc caccgccgtg cccgccgcgc cgccgccggc ctccagcgcc
     9481 gcggcgcaca tgcgccgccc ggcctccagg ctcggctcgc ccggcacgcc gagcagcagg
     9541 cggtcgccga cgcgcagcac cgtcacgtcc gcggccggtg accacggcgc cggcatcgcc
     9601 agcagcttgc ggccgtgcac cggcccggcc agcgcgcgcg gccgccgccg cccctccacg
     9661 aagccgtaca ggaagtgcgg gccgttggcc gccccgccca cgaacggcag gccgaaccac
     9721 gcgcgccgcg ccacccgctt gcccggctcc agctcctggc cgtcgtagcg cacgcgccgc
     9781 gccgcgcccg ccaccggcag gtcgccggcc aggttcgccc cggccgcgtc ccaggcctgg
     9841 cgcatcgccg cggccacgcg caggcccagg cggtcggcgc acgcgtgctg gttgcactcg
     9901 tccgcgcgcg ggatcaggtc gccctccgtg ccgttggtga agatcgccac gggcgccgcg
     9961 ccccctgaga
//
LOCUS       DATMKC010000400         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_316344, whole genome shotgun sequence.
ACCESSION   DATMKC010000400 DATMKC010000000
VERSION     DATMKC010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316344"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /gene="ftsH"
                     /locus_tag="VNU01_11660"
     CDS             complement(<1..1487)
                     /gene="ftsH"
                     /locus_tag="VNU01_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWH33315.1"
                     /translation="MNLQRLFRGPFIWITLLLVVAFATVSTLGSAAEPEELRFDEFMT
                     KLEAGDVKTVDDHAREFRLVGELDDGTRYRTNYNPQVDDETLGQALDEARRAGTIERT
                     VDNSQGNVFIQVLMSILPFVLIFGLFFFLMTQMQGGGNRVMSFGKAKPKLVNKDAPKV
                     TFADVAGSDEAVEELREIKDFLENPAKFQAMGAKIPKGVLLFGPPGTGKTLLARAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKANAPAIIFMDEIDAVGRHRGAGMG
                     GGHDEREQTLNQLLVEMDGFDVKTGVILIAATNRPDILDPALLRPGRFDRQIVVDRPD
                     LVGRKAILKVHARGKPLGPDADLEVIARRTPGFTGADLANLINEAALLSARKGNKEIT
                     MAALEDAIDRVIAGPERKTRLMGEVEKRIIAYHEGGHTIVGHALPHADPVHKVSIIPR
                     GRALGWTLTLPTEDKYLVSRGELIDRLAMMLGGRVAEEITIGDFTT"
     gene            complement(1653..2210)
                     /gene="hpt"
                     /locus_tag="VNU01_11665"
     CDS             complement(1653..2210)
                     /gene="hpt"
                     /locus_tag="VNU01_11665"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWH33316.1"
                     /translation="MAHDALHPDIERVLISSADIQDKLRALSAAIDRDYADRDVLLVG
                     VLKGAFIVMADLARYLTVPVEFDFMAVSSYGSATQTSGVVRILKDLDLDIAGRHVLVV
                     EDIVDSGLTLQYLLRNLRARGPASLEVMALLRKPDQIRVEIPVRYHGFDVENVFVVGY
                     GLDYAERYRNLPYVGTLKAEVYSHT"
     gene            complement(2252..>2773)
                     /gene="tilS"
                     /locus_tag="VNU01_11670"
     CDS             complement(2252..>2773)
                     /gene="tilS"
                     /locus_tag="VNU01_11670"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWH33317.1"
                     /translation="PQQPAADVRRLLRLAPGQAAHARGLRGSCGGGWLALGPDGIAAL
                     APRAVALPGVTPLAELDLALYADTERDPVDPRHVASGPPGARLPLHARLPVPADAELV
                     VRARCDGDRMRLRQGTRALQDVLTDAGVPRLVRDLLPVVVDARDEPVWVPGVAVARMP
                     ESLAHVRAWLAPF"
BASE COUNT          395 a          992 c          976 g          410 t
ORIGIN      
        1 gtggtgaagt ccccgatggt gatctcctcg gccacccgcc cgcccagcat catcgccagg
       61 cggtcgatca gctccccgcg gctgaccagg tacttgtcct cggtcggcag cgtgagcgtc
      121 cagcccagcg cgcggccgcg cgggatgatc gacaccttgt gcaccgggtc ggcgtgcggc
      181 agcgcgtggc cgacgatggt gtgaccgccc tcgtggtagg cgatgatccg cttctcgacc
      241 tcgcccatga ggcgcgtctt gcgctcggga ccggcgatca cgcggtcgat ggcgtcctcc
      301 agggccgcca tcgtgatctc cttgttgccc ttgcgggcgc tcagcagcgc cgcctcgttg
      361 atgaggttgg ccaggtccgc gccggtgaaa ccgggcgtgc ggcgcgcgat gacctccagg
      421 tcggcatcgg ggcccagcgg cttgccgcgc gcgtggacct tcaggatcgc cttgcggccc
      481 accaggtccg gccggtcgac cacgatctgg cggtcgaagc ggcccgggcg cagcagcgcc
      541 gggtccagga tgtcggggcg gttggtggcg gcgatgagga tcacaccggt cttgacgtcg
      601 aagccgtcca tctccaccag gagctggttc agcgtctgct cgcgctcgtc gtggccgccg
      661 cccatgccgg cgccgcggtg gcggccgacg gcgtcgatct cgtccatgaa gatgatcgcg
      721 ggcgcgttgg ccttcgcctg ctcgaacagg tcgcgcacgc ggctggcgcc cacgcccacg
      781 aacatctcca cgaagtcgga gccggaaatc gagaagaacg gcaccccggc ctcgcccgcc
      841 acggcgcgcg cgagcagcgt cttgccggtg ccgggagggc cgaacagcag cacgcccttg
      901 gggatcttgg cgcccatggc ctggaacttg gccgggttct ccaggaagtc cttgatctcc
      961 cggagctcct ccacggcctc gtccgagccg gcgacgtcgg cgaaggtgac cttcggcgcg
     1021 tccttgttca ccagcttggg cttggccttg ccgaagctca tgacccggtt gccgccgccc
     1081 tgcatctgcg tcatcaggaa gaagaacaac ccgaagatca ggacgaacgg caggatcgac
     1141 atcagcacct ggatgaacac gttgccctgg gagttgtcga ccgtgcgctc gatggtgccg
     1201 gcgcggcgcg cctcgtccag cgcctggccc agcgtctcgt cgtccacctg cgggttgtag
     1261 ttcgtgcggt agcgcgtgcc gtcgtccagc tcgccgacca ggcggaactc ccgggcgtgg
     1321 tcgtcgacgg tcttgacgtc gcccgcctcc agcttggtca tgaactcgtc gaagcgcagt
     1381 tcctccggct cggcggcgct gcccagcgtg gagaccgtgg cgaacgccac gaccagcagc
     1441 agggtgatcc agatgaacgg gccgcggaac agtcgctgga gattcatgaa atccctcgtc
     1501 gaaggggggt ggcggccatg ctagcaccgg ggttccggcc cgaccggggc cgcatcgtca
     1561 ggcttgtcca catcccccgc gccggtacgg gtgcgttttc agagtcatgg ctctgaaacc
     1621 gcacccgtca ggagtgcgac ggctcgcgcc gtctacgtgt ggctgtagac ctcggccttc
     1681 agcgtgccca cgtacggcag gttccggtag cgctccgcgt agtccagccc gtagcccacc
     1741 acgaacacgt tctccacgtc gaagccgtgg tagcgaacgg ggatctccac cctgatctgg
     1801 tccggcttgc gcagcagcgc catgacctcc agcgacgccg gcccccgcgc gcgcaggttg
     1861 cgcagcaggt actgcagggt caggccgctg tcgacgatgt cctccaccac caggacgtgg
     1921 cgcccggcga tgtccaggtc caggtccttg aggatgcgca ccacgccgga ggtctgcgtg
     1981 gccgagccgt agctggacac cgccatgaag tcgaactcga ccggcacggt gaggtagcgc
     2041 gccaggtcgg ccatcacgat gaacgcgccc ttgagcaccc cgaccagcag gacgtcgcgg
     2101 tcggcgtagt cgcggtcgat cgcggcactc agcgcgcgca gcttgtcctg gatgtccgcc
     2161 gaggagatca gcacccgctc gatgtcgggg tgcagcgcgt cgtgggccac ctgcgtgagt
     2221 cctgtcgtcg aacggggtgc ggggccagag cctagaacgg cgccagccag gcgcgcacgt
     2281 gcgcgagcga ctccggcatg cgggcgaccg ccaccccggg aacccacacc ggctcgtcgc
     2341 gcgcgtccac caccacgggc agcaggtcgc gcaccaggcg aggcacgccc gcgtcggtca
     2401 gcacgtcctg gagcgcgcgg gttccctgac gcaggcgcat gcggtcgccg tcacagcgcg
     2461 cgcgcacgac cagctccgcg tcggccggga ccggcagccg cgcgtgcagc ggcaggcgcg
     2521 cgccgggcgg gccggaggcg acgtggcggg ggtcgacggg gtcgcgctcg gtgtccgcgt
     2581 acagcgccag gtccagttcc gccagcggtg tgacacccgg cagcgccacc gccctgggcg
     2641 ccagggcggc gatgccgtcc ggccccaggg ccagccagcc gccgccgcag gagccgcgca
     2701 gcccgcgcgc atgcgccgcc tggccgggcg ccaaccgcag cagccggcgc acgtccgccg
     2761 ccggctgctg cgg
//
LOCUS       DATMKC010000401         2687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_57119, whole genome shotgun sequence.
ACCESSION   DATMKC010000401 DATMKC010000000
VERSION     DATMKC010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57119"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VNU01_11675"
     CDS             <1..212
                     /locus_tag="VNU01_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33318.1"
                     /translation="VEPDATAPAAGEQDGSAGSVGSEVAVEDPTALTGGWEGGTPNTG
                     TAISAGGLLLLAVGAGLLWLRRART"
     gene            218..814
                     /gene="purN"
                     /locus_tag="VNU01_11680"
     CDS             218..814
                     /gene="purN"
                     /locus_tag="VNU01_11680"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWH33319.1"
                     /translation="MPSRLVVLISGSGSNLQALLDRGPDLGGQIALVVSDRHDAYGLE
                     RAAAAGVPAAVVSPKDHADRPAWEAALGDAVAAAAPDLVVLAGFMRILPAAFVSRWPV
                     LNVHPSLLPAFPGARAVEEALAWGVKLTGCTVHFVDEKVDNGPIVAQECIPVLPGDTP
                     ESLHERIKAVEHRLLPECVTLFCRGRIAVDGRHVRIDK"
     gene            811..2358
                     /gene="purH"
                     /locus_tag="VNU01_11685"
     CDS             811..2358
                     /gene="purH"
                     /locus_tag="VNU01_11685"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWH33320.1"
                     /translation="MSDVTPVRRALVSVYDKTGLAELAAGLAQAGAAIVSTGSTAQAI
                     RDAGVAVTDVSEVTGFPEIMDGRVKTLHPAVHAGILADRSKDTHLAALDEHGIAGIDL
                     VVVNLYPFRETIADPDVTPERAIEMIDIGGPTMVRAAAKNHAHVAVVVQPEDYAAVLA
                     EVGERGGVTAATRRRLAAKAFAHTAAYDADVASWFARERSERFPERLTTRFHLAQTLR
                     YGENPHQQAAYYVEAGQPWGLGSATQLHGKELSYNNLLDTDAAWGMAADFAEPCVAII
                     KHTNPAGLAVAASLAEAYPLALAGDPVSAFGGIVAVNRAMDAATARQVVEVFTEVVVA
                     PGYDEEALEVLRGKANLRILEVTRPRPPQDALSLRSVSGGLLVQDVDAAAEDPASWTV
                     ATRAEPDDATLAELAFAWKVCKHVKSNGIVLTKDRAAVGVGAGQMSRVDSVRIAVDKS
                     DGRSKGAVLASDAFFPFPDGPRAAIEAGVVAIVQPGGSVRDEEVVAVCDEAGIPMVLT
                     GTRHFRH"
     gene            2416..>2687
                     /locus_tag="VNU01_11690"
     CDS             2416..>2687
                     /locus_tag="VNU01_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33321.1"
                     /translation="MSGYDGYQDHDGDYDEPGAEQERPARGRGGMGGATPLGAGLAGL
                     LVGALLAGIVLIAVGGNPFSDANDVTYREIVIEDISDAGDQVCWAPA"
BASE COUNT          363 a          977 c         1013 g          334 t
ORIGIN      
        1 aagtcgagcc ggacgccacc gctccggccg cgggtgagca ggacgggtcc gccggctcgg
       61 tgggctccga ggtggcggtg gaggacccca ccgccctcac gggcggctgg gagggcggca
      121 cgcccaacac cggcacggcg atctccgccg gcggcctgct gctgctcgcc gtcggcgccg
      181 gcctgctgtg gctgcgccgc gcccgcacct gaccgccgtg ccttctcgcc tggtcgtcct
      241 catctccggt tccggcagca acctgcaggc gctgcttgac cggggtcccg acctcggcgg
      301 gcagatcgcc ctggtggtgt ccgaccgcca cgacgcgtac gggctggagc gggcggccgc
      361 cgcgggggtg ccggccgcgg tcgtctcacc gaaggaccac gccgaccgcc ccgcgtggga
      421 agccgcgttg ggcgacgccg tcgcggccgc cgcacccgac ctcgtggtgc tggcagggtt
      481 catgcgcatc ctcccggcgg cgttcgtgtc gcgctggccc gtgctgaacg tccatccgtc
      541 cctgctgccg gcgttccccg gcgcgcgagc ggtggaggag gcgctggcct ggggcgtgaa
      601 gctgaccggc tgcaccgtgc acttcgtgga cgagaaggtc gacaacgggc cgatcgtcgc
      661 ccaggagtgc atccccgtgc tgcccggcga cacccccgag tccctgcacg agcgcatcaa
      721 ggcggtggag caccgcctgc tgccggagtg cgtcaccctg ttctgccgag gccgcatcgc
      781 ggtcgacggc cgtcacgtga ggatcgacaa gtgagcgacg tcacccccgt ccgccgcgcg
      841 ctggtgagcg tgtacgacaa gaccggcctg gccgagctgg ccgcgggcct ggcgcaggcc
      901 ggcgcggcga tcgtctccac cggctcgacc gcgcaggcca tccgcgacgc cggcgtcgcc
      961 gtcaccgacg tgtcggaggt caccggcttt cccgagatca tggacgggcg cgtgaagacg
     1021 ctgcaccccg ccgtgcacgc ggggatcctg gcggaccgct ccaaggacac gcacctggcg
     1081 gcgctcgacg agcacggcat cgccggcatc gacctggtgg tggtgaacct gtatccgttc
     1141 cgcgagacga tcgcggaccc ggacgtcacg ccggagcgcg ccatcgagat gatcgacatc
     1201 ggcgggccca ccatggtccg cgcggccgcc aagaaccacg cgcacgtcgc cgtggtggtg
     1261 cagccggagg actacgccgc ggtgctggcg gaggtgggcg agcgcggcgg cgtgacggcg
     1321 gccacccgcc ggcgcctggc cgcgaaggcg ttcgcgcaca ccgccgccta cgacgccgac
     1381 gtcgccagct ggttcgcgcg cgagcgctcc gagcgcttcc ccgagcggct caccacccgc
     1441 ttccacctgg cccagaccct gcgctacggc gagaacccgc atcagcaggc cgcctactac
     1501 gtggaggccg gccagccctg gggcctgggc agcgcgacgc agctgcacgg caaggagctg
     1561 agctacaaca acctgctgga caccgacgcg gcctggggca tggcggccga cttcgccgag
     1621 ccgtgcgtgg cgatcatcaa gcacaccaat cccgccggac tggcggtggc ggccagcctc
     1681 gcggaggcct acccgctggc gctggccggc gacccggtga gcgcgttcgg cggcatcgtg
     1741 gcggtgaacc gcgccatgga cgccgcgacc gcgcgccagg tggtggaggt gttcaccgag
     1801 gtcgtggtgg cgcccggcta cgacgaggag gcgctggagg tgctgcgcgg caaggcgaac
     1861 ctgcgcatcc tcgaggtcac gcgcccgcgg ccgccgcagg acgcgctcag cctgcgcagc
     1921 gtgagcgggg gcctgctggt gcaggacgtc gacgccgcgg cggaggaccc ggcgagctgg
     1981 acggtcgcca cccgcgccga gccggacgac gccacgctgg cggagctcgc gttcgcgtgg
     2041 aaggtctgca agcacgtgaa gtccaacggc atcgtgctca ccaaggaccg tgccgcggtc
     2101 ggtgtgggtg cgggccagat gtcgcgcgtg gacagcgtgc gcatcgcggt ggacaagtcc
     2161 gacgggcgca gcaagggcgc cgtgctggcc agcgacgcgt tcttcccctt cccggacggg
     2221 ccccgcgccg ccatcgaggc gggcgtcgtg gcgatcgtcc agccgggcgg cagcgtgcgg
     2281 gacgaggaag tcgtcgccgt gtgcgacgag gccgggatcc cgatggtcct cacgggaact
     2341 cgccacttcc gccactaggc tcggcggaac acgacgcctc gcccaggacg gcggacgcgc
     2401 acgggaggtg tgccgatgag cggctacgac ggctaccagg accacgacgg cgactacgac
     2461 gagccgggcg cggagcagga gcgcccggcg cgcgggcgcg gcggcatggg tggtgccacg
     2521 ccgctcggcg cggggctggc cggcctgctc gtcggcgcgc tgctggccgg catcgtgctg
     2581 atcgcggtgg gcggcaaccc gttctccgac gccaacgacg tcacctaccg cgagatcgtc
     2641 atcgaggaca tcagcgacgc gggcgaccag gtctgctggg cgcccgc
//
LOCUS       DATMKC010000402         3103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_152417, whole genome shotgun sequence.
ACCESSION   DATMKC010000402 DATMKC010000000
VERSION     DATMKC010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152417"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VNU01_11695"
     CDS             complement(<1..872)
                     /locus_tag="VNU01_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWH33322.1"
                     /translation="MCSTQGSDESAGPLPLLQQAAELLRQAGSLPLGGAVSDVELTQA
                     VQCLEALGRMAESRKCAVVREIEQRHAARGQSAGSTEDLLTRVLHLTPGEARARTELA
                     AGLKQLPDTAAAFADGRMGLGQAAVAVRKAAEVAGRDDAEVLLATIDRTASTVGQTVN
                     RTRLAREIDTAVAKTGVDVLAERERRAFQDRKLQWSVRDGMDVLTAHLDPVGAATVRG
                     MLDALSDKTSERDSRSFVQRQADALVDIATLAGHERARQQQRDGRGLGQQTLQSGNVL
                     LITTSEGLSAVLCKG"
     gene            complement(951..1457)
                     /locus_tag="VNU01_11700"
     CDS             complement(951..1457)
                     /locus_tag="VNU01_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWH33323.1"
                     /translation="MSATATPDPAEQRVIDALVRLGIPHELVRIDPAFAATADFCREY
                     GFPMEASVNCIVVMGKAEPPLFAACMVQATRRLDVNGAIKRRFGVRKASFAPSDVAVE
                     LTGMLPDGVTPVGLPDGLPVWVDAPVMELDRIIVGGGSRALKILLPAGDLLRLPGAEA
                     VDGLSRAD"
     gene            1539..2231
                     /locus_tag="VNU01_11705"
     CDS             1539..2231
                     /locus_tag="VNU01_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33324.1"
                     /translation="MYVLAVLLVLVGLGLQGSQERRETHRDVPNAIELACTEAGVEVR
                     RGAAAAGPDGVVMRFAHTEGDWVMTWEGAPGSWSSSGSAQTAEMVWPVPPGSVRVQCY
                     PGHLSLRDPSHEARWWADVEVVDPAGHYRRAESSCRQRRAASWHGRAVPVAQERAIVV
                     EALGMLDAHQRAGDELVRLGYPQGIPRDWGLLRDGEVIANVRMAMRSDGQVSPSGMSA
                     CADGASGQSSTS"
     gene            complement(2213..>3103)
                     /locus_tag="VNU01_11710"
     CDS             complement(2213..>3103)
                     /locus_tag="VNU01_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWH33325.1"
                     /translation="KKPPMSTIGSSVTAAHAVIATLLPIVDIGGFFAKAHPTRSYGIA
                     VRLSAPPPEGMHISVDAPTRSTRPWRGAEHGMVVVGESHKTGHGDDLDGHYRALEQWA
                     REHFAVTEVSYRWSAQDYRPVDELPYVGRCPRTERVYVGTGFMKWGLSNGTAAGAMIA
                     DLIGGRDTAWLPAFDASRAGVSDAQAAKTAVRENLEVGKRFVADRVGRLSAEDLGALS
                     PGEGGIVRVGDKAVAAYRDPDGGVHAVSPTCTHLGCTVSWNDAERSWDCPCHGSRFSV
                     EGAVLDGPAVSPLEPYEVED"
BASE COUNT          431 a         1102 c         1123 g          447 t
ORIGIN      
        1 cccttacaca aaaccgctga caggccctcc gaggtcgtga tcagcagcac gttgccggac
       61 tgcaacgtct gctgccccag cccccgcccg tcccgctgct gttgacgggc gcgctcgtgc
      121 ccggccagcg tcgcgatgtc gaccagcgcg tcagcttggc gttgcacgaa gctgcggctg
      181 tcacgctctg acgtcttgtc cgacagggcg tccagcatcc cgcgcaccgt cgccgccccg
      241 acggggtcga ggtgtgcggt gagcacgtcc atgccgtcac gcacggacca ttggagcttg
      301 cggtcctgaa acgcgcggcg ttcccgctcc gccaacacat cgacaccggt tttggcgacc
      361 gcggtgtcga tctcgcgggc caggcgggtg cggttcacgg tctggccgac cgtcgacgcc
      421 gtgcggtcaa tcgtggcgag cagcacctcg gcgtcgtcac ggccggccac ttcggcggct
      481 ttgcgcaccg ccacggcggc ctggcccagg cccatgcggc cgtcggcgaa cgccgccgcc
      541 gtgtccggca actgcttcag ccccgcggcc agttccgtcc gcgccctcgc ctcaccaggg
      601 gtgaggtgca gcacgcgcgt cagcaggtcc tcggtggacc cggcggactg gccgcgggcg
      661 gcgtggcgct gctcgatctc gcgcaccacc gcgcacttgc gggactccgc catccgaccc
      721 agcgcctcca ggcattgcac cgcctgcgtg agctcgacgt cagacaccgc tccgccaagc
      781 ggcaggctgc cggcttggcg cagcagttcg gcggcctgct gcaacagcgg cagcggcccc
      841 gcgctctcgt cggacccttg agtcgaacac atgttcgata ccatacaacg cgactgtgac
      901 aggtcaaccc acgaaattga atctgtgggc aacccgtgca gtaggagtgg tcagtcggcg
      961 cgagacagcc cgtccacggc ttccgcgccg ggcagccgca gcaggtcgcc ggcaggcagc
     1021 aggatcttca gcgccctgct gccgccgccc acgatgatcc ggtccagctc catcacgggc
     1081 gcgtccaccc acaccggcaa cccgtcgggc aggcctaccg gcgtcacccc gtcgggcagc
     1141 atgccggtca attccacggc gacgtcgctc ggcgcaaacg acgccttgcg cacaccgaag
     1201 cgccgtttga tggcgccgtt gacgtccaga cgtcgcgtcg cctgcaccat gcacgcggcg
     1261 aacagcggcg gctcggcctt gcccatgacc acgatgcagt tgaccgacgc ctccatcggg
     1321 aagccgtact cgcggcagaa gtcggcggtg gccgcgaacg ccgggtcgat ccgcaccagc
     1381 tcgtggggga tgcccaaccg caccagcgcg tcgatgacgc gctgctcggc cgggtcgggt
     1441 gtggcggtcg cactcatcgt gtcatcctgc ccagggaacc ggtgcgggcg ccaacgcgtc
     1501 tctctcgcag gtggactccc acgggggaag gagagccgat gtacgtgctg gcggtgctgc
     1561 tcgtgctggt ggggctggga ctgcagggca gccaggaacg gcgggagacc catcgggacg
     1621 tcccgaacgc gatcgagctg gcgtgcacgg aggcgggcgt ggaggtgcgc cggggtgcgg
     1681 cggcggcggg cccggacggg gtggtgatgc gtttcgcgca cacggagggt gactgggtga
     1741 tgacctggga gggcgcgccc ggttcatggt cgtcctctgg cagcgcgcag acggcggaga
     1801 tggtgtggcc ggtcccgccg ggctccgtcc gcgtccagtg ctatcccggc cacctgtcgt
     1861 tgcgcgaccc gtctcacgag gcccgctggt gggcggatgt cgaggtcgtc gatccggccg
     1921 ggcactaccg ccgggcggag agcagttgcc gacaaagacg tgccgcgtcg tggcacgggc
     1981 gtgccgtgcc ggtggcgcag gagcgcgcca tcgtcgtgga ggcgctcggc atgctggacg
     2041 cgcatcagcg cgcaggggac gagctggtcc ggcttggcta cccgcagggc atcccgaggg
     2101 attgggggct gctccgcgac ggcgaggtga tcgcgaacgt caggatggcc atgcgcagcg
     2161 acggacaggt ctcgcccagc ggcatgtcgg cctgcgccga cggcgccagc ggtcagtcct
     2221 cgacctcgta gggctccagg ggcgagaccg ccgggccgtc gagcacggcg ccctccacgg
     2281 agaagcgcga gccgtggcag gggcagtccc agctgcgctc ggcgtcgttc cagctgaccg
     2341 tgcagcccag gtgcgtgcag gtgggactga cggcgtgcac accaccgtcg ggatcgcgat
     2401 acgccgccac cgccttgtcg ccgacgcgca cgatgccgcc ctccccgggg gacagggcgc
     2461 cgaggtcctc ggcgctgagc cggccgacac ggtcggcgac gaagcgcttg ccgacctcca
     2521 ggttctcccg cacggcggtc ttggcggcct gggcgtcgga cacgccggcg cgtgaggcgt
     2581 cgaaggcggg cagccacgcg gtgtcccggc cgccgatcag gtcggcgatc atcgcgcccg
     2641 cggcggtgcc gttggacagc ccccacttca tgaagccggt gccgacgtac acccgctcgg
     2701 tgcgcgggca gcggcccacg tacggcagct cgtccaccgg tcggtagtcc tgcgcggacc
     2761 agcggtagga gacctcggtg accgcgaagt gctcgcgggc ccactgctcc agcgcgcggt
     2821 agtgcccgtc gaggtcgtcg ccatggccgg tcttgtggct ctcgcccacc acgaccatgc
     2881 cgtgctcggc gccgcgccac gggcgcgtgg agcgcgtcgg cgcgtccacc gagatgtgca
     2941 tgccctccgg tggcggcgcc gacagccgca cggcgatgcc gtacgaacgg gtcgggtggg
     3001 ccttggcgaa gaagcccccg atgtccacga tcggcagcag cgtcgcgatc acggcgtggg
     3061 cggcggtgac gctgctgccg atcgtggaca tcgggggctt ctt
//
LOCUS       DATMKC010000403         4438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_311290, whole genome shotgun sequence.
ACCESSION   DATMKC010000403 DATMKC010000000
VERSION     DATMKC010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311290"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..773)
                     /locus_tag="VNU01_11715"
     CDS             complement(528..773)
                     /locus_tag="VNU01_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWH33326.1"
                     /translation="MHETVHITSLAARLPAIVEQLRTGERVVIADDSGPVGALVSLWD
                     LESLDETDAVLADKGLLQGLRESQALAGEPPSPDYEP"
     gene            complement(833..2752)
                     /locus_tag="VNU01_11720"
     CDS             complement(833..2752)
                     /locus_tag="VNU01_11720"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HWH33327.1"
                     /translation="MSTYSAKSITVLEGLEAVRKRPGMYIGSTDARGLHHLVWEVVDN
                     AVDEHLAGHAARIVVTVRTDGGVEVRDDGRGIPVENHPKERKPAVEVVLTKLHAGGKF
                     DQQSYAVSGGLHGVGVSVVNALSRKTEVEVARGGKVHAITFSRGKRTKALHVVGKARK
                     TGTVVRFWPDEDIFDTLEFDDATVCTRLRETALLNPGLRIDYSDERTGFSETFQFRKG
                     LPDFVAELLDGDSPLLSKAVTISREETVDGKALAADIAVNWSETGFDERVRSYVNIIR
                     TADGGAHEEGFRKAITGTLNRWAEANKALRRGEDKVSGDDLREGMVAVVSVKMPDPQF
                     EGQTKGKLGSAIMRGFVERCVNEAFAEWLDANPAQARKIVRKAQVSAKAREAARHARD
                     LTRRKGILDSASAGLPGKLADCSSRSPEECELYLVEGDSAGGSSKMARDRQTQAILPL
                     RGKVLNVEKAQLRKVLSNAEIQNVIKAIGTGIGDDFDYSRLRYHKVVLMSDSDVDGGH
                     ITTLLLTFFYRLMPKLVDGGHLYLAQPPLYQLRKGTRTVYAMNDRERDRVLKTDFKGE
                     VRVDISRFKGLGEMSPQQLWDTTMDPAKRTLLRVTVPHAEEADRIFTLLMGESAADRR
                     EWIEAKARYVDNLDV"
     gene            complement(2840..3499)
                     /locus_tag="VNU01_11725"
     CDS             complement(2840..3499)
                     /locus_tag="VNU01_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33328.1"
                     /translation="MRRVLSVTALALMAALSLSGCGREASVTLSDGSLPGESSGERSM
                     TGDPDDTLGGLEPEENEPAQDGGTAVEPTGDPDAPMKGAPPPDDPDGCPECTTSTTPA
                     EPVDEPGDRPGDNSGDGGYTTPRPGQEDVRAREWETAEVADDDVTVTLTWWSGVEPCT
                     VLDRVDVVEQADKVVITLYEGNAPGDEGACPAIAVERRTRVTLSAPLAGRAIVDGAKA
                     K"
     gene            complement(3590..3802)
                     /locus_tag="VNU01_11730"
     CDS             complement(3590..3802)
                     /locus_tag="VNU01_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33329.1"
                     /translation="MSEHEQPDASRDADLDPAADTTMFQAFVQRAEQGQQDPRTGKPV
                     GAPFRLATLLVGLGVFAGLVWLLLRI"
     gene            complement(3840..>4438)
                     /locus_tag="VNU01_11735"
     CDS             complement(3840..>4438)
                     /locus_tag="VNU01_11735"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HWH33330.1"
                     /translation="MGALHAEVVFSGRAAHSARPWHGDNALSKAGAFLTAMRERTAED
                     VAVDGLVYREVVTPTRAWTEPVREARNVVPDRFTVNCNYRFAPDKSVADAERRLAEVV
                     AAASDGAAEVRVVDRAPAGRPHRGDRLVARFIETVGAPVEPKQAWTDVARFSEAGVPA
                     LNYGPGLTAQAHQAGEHVPEANLEVARQALASFLAGSG"
BASE COUNT          563 a         1645 c         1525 g          705 t
ORIGIN      
        1 ggcgtgcgcg gcggcgatga gctcctccgg gctggtctgc ccgtcggggt tctcggcgcg
       61 cgacggccag ctcaccggca gcccgtccac ggcgttcgac gagtcgaacg tcacggtgcc
      121 cttgcccgtg gccagctccc cttcccagac ggtgctcgcg atcctgtccg gcatgtcgtc
      181 ctcctgcgtg gcggtggcga ttccctgacc gcgcaaccta ccaaccgcgc gacatcgacg
      241 cggtcggatc cgacggagtg acggtcaccg ccgccggtac gacgtcgccc ggtgcgccac
      301 cgcggtcact tcgacgccgc ctgtctcgtt aatccgatac agcacccggt acggaccccg
      361 gcgcgcacag aactccccga gcagcggagg ttgcagtggc acacccaagc gaaacggttg
      421 ctccgctacg tgggcgatga gctcccacat ggctggttga accttcagcg gaagacgctc
      481 aatttggcga cgggcgggcc ggctgacgtg gaagccggcg gccacggtca gggctcgtag
      541 tcgggactcg ggggttcgcc tgccagcgcc tgcgactctc tcagcccttg aaggaggccc
      601 ttgtcggcca gcacggcatc ggtctcgtcg aggctttcca ggtcccagag ggacacgagc
      661 gcaccgacgg gaccactgtc gtcagcgatc acgacgcgct ctcccgtgcg cagctgctcc
      721 acgatcgcgg ggaggcgggc ggccagagaa gtgatgtgga cggtctcatg catggcagga
      781 ctgtcatccc gtcccgcgat tgggtcaatg ccttcgtcca caggcacctt ggctacacgt
      841 cgaggttgtc gacgtagcgg gccttcgcct cgatccactc gcggcggtcg gcggccgact
      901 cgcccatgag cagcgtgaag atgcggtcgg cctcttccgc gtgcggcacg gtcacgcgca
      961 gcagcgtgcg cttggccggg tccatggtgg tgtcccacag ctgctgcggg ctcatctccc
     1021 ccaggccctt gaaccgtgag atatccacgc ggacctcgcc cttgaagtcc gtcttcagca
     1081 cccggtcgcg ctcgcggtcg ttcatcgcgt agacggtgcg ggtgcccttg cggagctggt
     1141 acagcggcgg ctgcgccagg tacaggtggc cgccgtccac cagcttcggc atcagccggt
     1201 agaagaacgt gagcaggagc gtggtgatgt ggccgccatc aacatcgctg tcggacatca
     1261 gcaccacctt gtggtagcgc agccgcgagt agtcgaagtc gtcgccgatg cccgtgccga
     1321 tggccttgat gacgttctgg atctcggcgt tgctcagcac cttgcgcagc tgggccttct
     1381 ccacgttgag caccttgccg cgcagcggca ggatcgcctg ggtctggcgg tcgcgcgcca
     1441 tcttggagct gccgccggcg gagtcgccct cgaccaggta cagctcgcac tcctccgggc
     1501 tgcgcgacga gcagtccgcg agcttgcccg ggagccccgc cgaggcggag tcgaggatgc
     1561 ccttgcgccg cgtgaggtcg cgcgcgtggc gcgccgcctc gcgcgccttg gccgacacct
     1621 gcgccttgcg cacgatcttg cgcgcctgcg ccgggttcgc gtccagccac tcggcgaacg
     1681 cctcgttcac gcagcgctcc acgaagccgc gcatgatcgc cgagccgagc ttgcccttgg
     1741 tctgcccctc gaactgcggg tcgggcatct tcaccgacac cacggcgacc atgccctcgc
     1801 gcaggtcgtc gccggagacc ttgtcctcgc cgcggcgcaa ggccttgttg gcctccgccc
     1861 agcggttgag cgtgccggtg atcgccttgc ggaagccctc ctcgtgcgcg ccgccgtcgg
     1921 ccgtgcggat gatgttgacg tagctgcgca cgcgctcgtc gaagccggtc tccgaccagt
     1981 tcaccgcgat gtcggcggcc agcgccttgc cgtccaccgt ctcctcgcgc gagatcgtca
     2041 ccgccttgga cagcagcgga ctgtcgccgt cgagcagctc ggcgacgaag tccggcaggc
     2101 ccttgcggaa ctggaaggtc tcgctgaagc cggtgcgctc gtcgctgtag tcgatgcgca
     2161 gcccggggtt gagcagcgcc gtctcgcgca ggcgcgtgca caccgtggcg tcgtcgaact
     2221 ccagggtgtc gaagatgtcc tcgtccggcc agaagcgcac cacggtgccg gtcttgcgcg
     2281 ccttgccgac cacgtgcagc gccttcgtgc gcttgccgcg cgagaacgtg atcgcgtgca
     2341 ccttgccgcc ccgggcgacc tccacctcgg tcttgcgcga cagcgcgttc acgaccgaca
     2401 cgccgacgcc gtgcaggccg cccgagacgg cgtaggactg ctggtcgaac ttgccgccgg
     2461 cgtgcagctt ggtgagcacg acctcgacgg cgggcttgcg ctccttgggg tggttctcca
     2521 ccgggatgcc gcggccgtcg tcgcggacct cgacgccccc gtccgtgcgc acggtcacca
     2581 cgatgcgcgc ggcgtggccg gccaggtgct cgtccacggc gttgtcgacc acctcccaga
     2641 cgaggtggtg caggccccgg gcgtccgtgg agccgatgta catgccggga cgcttgcgca
     2701 cggcctcgag gccttcgagg acggtgatcg acttggcgga gtaggtgctc acgtggggtg
     2761 tggctttccg ttccgagcag cggacagggc cggggccatt gtgccccgac cctgtcccag
     2821 gaccccgcaa gccggggcgc tacttggcct tggcgccgtc cacgatcgcg cggcccgcca
     2881 ggggcgccga cagcgtcacg cgcgtgcgcc gttcgaccgc gatcgccggg caggcgccct
     2941 cgtcgccggg cgcgttgccc tcgtacagcg tgatgaccac cttgtccgcc tgctcgacca
     3001 cgtccacgcg gtccagcacg gtgcacggct ccacgccgga ccaccaggtg agcgtcacgg
     3061 tgacgtcgtc gtcggcgacc tcggcggtct cccactcgcg ggcccgcacg tcctcctggc
     3121 ccggccgcgg ggtggtgtag cccccgtccc ccgagttgtc gccggggcgg tcccccggct
     3181 cgtccacggg ctccgccggc gtggtggagg tggtgcactc cgggcacccg tccgggtcgt
     3241 ccggcggcgg cgcgcccttc atcggcgcgt ccgggtcgcc cgtcggctcg accgcggtcc
     3301 cgccgtcctg cgccggctcg ttctcctccg gctccaggcc gcccagcgtg tcgtcggggt
     3361 cgccggtcat cgagcgctct cccgagcttt ccccgggcag cgagccgtcg ctcagtgtca
     3421 ccgacgcctc acggccgcat cccgacaggg acagcgcggc catcagggcc agcgcggtga
     3481 cgctcaggac ccgccgcatc gtctcgctcc tctcgcaccg cctccgatgc cgggtcagac
     3541 gcgcgaaggc cgcggccggt tcccgtcgcg accgcctttc gcagccaatt cagatgcgca
     3601 ggagcagcca gacgaggccg gcgaagacgc ccagtcccac gagcagcgtc gccaggcgga
     3661 acggcgcgcc gaccggcttg cccgtgcgcg ggtcctgctg gccttgctcg gcccgctgca
     3721 cgaacgcctg gaacatcgtg gtgtcggccg cggggtcgag gtcggcgtca cgcgacgcgt
     3781 cgggctgctc gtgctcgctc atgggcgcgc atggtagccg ggagctagca ccgcgcgcgt
     3841 caaccgctcc cagctaggaa ggaagcgagc gcctggcgcg caacctcgag gttggcttcc
     3901 gggacgtgct caccggcctg gtgggcctgg gcggtcagtc ccggaccgta gttcagcgcc
     3961 ggcacgccgg cctcgctgaa gcgtgcgacg tcggtccatg cctgtttggg ctcgaccggc
     4021 gccccgaccg tctcgatgaa acgcgccacc agccggtcgc cgcgatgtgg ccggcccgcg
     4081 ggcgcccggt ccacgacgcg cacctcggcc gcgccgtccg acgccgcggc caccacctcg
     4141 gccagccggc gctccgcgtc cgccaccgac ttgtccggcg cgaagcggta gttgcagttc
     4201 accgtgaagc ggtccggcac gacgttgcgc gcctcgcgca ccggctcggt ccaggcgcgc
     4261 gtcggcgtga ccacctcccg gtacaccagg ccgtccaccg ccacgtcctc ggcggtgcgc
     4321 tcgcgcatcg cggtcaggaa ggcccccgcc ttgctcagcg cgttgtcgcc gtgccacggc
     4381 cgggccgagt gggcggcgcg gccgctgaac accacctcgg cgtgcagcgc gcccatgc
//
LOCUS       DATMKC010000404        11529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_272699, whole genome shotgun sequence.
ACCESSION   DATMKC010000404 DATMKC010000000
VERSION     DATMKC010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272699"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /gene="menD"
                     /locus_tag="VNU01_11740"
     CDS             <1..1635
                     /gene="menD"
                     /locus_tag="VNU01_11740"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HWH33331.1"
                     /translation="AVLAPGSRSAPLAMALHADGRVRLHVRVDERSAAFLALGLAKAS
                     GRPAAVLCTSGTAAANFHPAVLEAHESRVPLLVLTADRPPELRGTGANQTTDQVKLYG
                     QAVRWFCEVGVPEARAGMVAYWRATACRAWAEAAGSPAGPVHLNVALREPLTPEPGDA
                     EFPEPLDGRADGAPWVAVESRTTVPDADLVEDLAARAASVMRGALVVGETSADPYELV
                     AFADRLGWPVIAEAHSGARQGPSAVAMYHHLLSDPSFADAHRPDFVVRVGRTGLSRPL
                     EAWLRTAGWHTLIDRDAAWLDPGRSVSEIIVAEPGQLLGMVGAALADLESDDGVEDWN
                     ASWGAADRRARDAVNQALDAEGLTEPRVARDVAASLPPGATLVVGSSMPIRDLDQTMA
                     PRGDVRVLANRGVSGIDGFVSTAVGVALAAGPAVALCGDLSLLHDANGLLGADADLTL
                     VVVNNDGGGIFSFLPQARHPDGFERLFGTPHGADLEALARAYGAAYTRVTAPGELRDL
                     VAAPRGVRLAEVRTDRAANVALHQRLRDAVSQALAG"
     gene            complement(1632..2876)
                     /locus_tag="VNU01_11745"
     CDS             complement(1632..2876)
                     /locus_tag="VNU01_11745"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HWH33332.1"
                     /translation="MTLRFGTADRPDVRAGTVAELLALSPGERAGEPLAWLRDGTGFV
                     GWGQAWRLDPGAGPERFARAGAALAEAFAGWDGRGAAPVAMAAFTFDPAAHGSFVRVP
                     ELLVHSGAAARAVAAGAGPDARPDALAPLAATVLPERATADRVRYAGASLPDLLWLDA
                     VAAATRLIDKGDIDKVVLARDHAVWSEAPFEPRVLAARLAARFPGCFTFVAEGLVGAS
                     PEMLVRRRGTRVESLVLAGSAARGDGAADDAARGDALLASAKDRAEHAFAVASVREAL
                     APLCRTLESDAEPSLLRLDNVQHLATRFGGTLAEPAPSALALAGALHPTAAVCGTPTA
                     EALEVIRELEGMERGRYTGPVGWVDARGDGEFAIALRCAELSGARARLFAGAGIVAAS
                     LPEAELEETRLKLRAMQSAFAD"
     gene            complement(2873..3400)
                     /locus_tag="VNU01_11750"
     CDS             complement(2873..3400)
                     /locus_tag="VNU01_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWH33333.1"
                     /translation="MTASSPAADAPQPELDSADAALAALLRGGDSGDGERLSRAALAE
                     RAGVPESLLEAMEREGLLQPGEDGYGEDDARVLAAGMELLAAGLPLAELLALARRHDE
                     AMRAVAEEAVELFVRFVRDPIRGTAEDAEEAGRRMRDAFERMLPATSGLVAHHFRRTL
                     LAAARERLRRETQAP"
     gene            complement(3441..3842)
                     /locus_tag="VNU01_11755"
     CDS             complement(3441..3842)
                     /locus_tag="VNU01_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33334.1"
                     /translation="MPLRRKNARVTAAGAPAGPGTLACPLCGEVVPVGEDGRCDLGHR
                     VQPAHAESETTQPLDTAHDAEPSPPRERPPEVSVAPVAGPAAAGLALADEDGPDTAGA
                     PDEDGGAAGPEDDADADADAGDTDFSGELDW"
     gene            3924..5234
                     /locus_tag="VNU01_11760"
     CDS             3924..5234
                     /locus_tag="VNU01_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWH33335.1"
                     /translation="MPPILYSADVVCPMSGPPLAQGGVLVDGDRVAAVGPSASLRGDA
                     AREHRIPGVLLPGMVNAHSHLEYADAAELARPCPFVQWVLDLGRLVQGWDADRWSRSA
                     HRGAQATLRGGATAVCDIVTKGPAVPAAGTLGLGGLSWVELAFVDWEHHDDVLAKLRA
                     ALELPAHGRVVGLAPHAPYSVGTGVLQAVAALARERGVPLHTHAAETREEVEALRQGT
                     GPLAQLARGRGMDFEWLDGGTGLSPVRYLDACGALFPGASVAHGVWVDGEEAALLAER
                     GAAVVLCPRSNALLSAGDAPLERYAEAGTALALGTDSAASCPDLDVLGEAHAWVELAR
                     RRGLDAWPADGGPRELAEQAIRLATVDGAAAMGLGAAAGVIEPGRRADLVGVAVDTTV
                     EDVWRDLVAHGPGQAVCTVVAGVRKARREPGTPWPEIDRQEWRA"
     gene            5231..5950
                     /locus_tag="VNU01_11765"
     CDS             5231..5950
                     /locus_tag="VNU01_11765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWH33336.1"
                     /translation="MSPDLPDPRPGPQKQAALVQAMFDRVAPRYDLANTLLSLGQDRH
                     WRRVAARAAEVGPEDVVADVAAGTGALSRELQAHGARVVALDFSLNMLRAGATRDDTA
                     DRIAWVNGDGMRLPLPDAGVDAVTIGFGLRNLPDPQAGLAEFARVVRPGGRLVVLEFS
                     HPVWPPLRAVYTRYLVGALPHAAKLVTSDPAAYRYLAESIQEWPGQAELAELIAKAGW
                     TGVRWKNLTGGIVAVHQAVRP"
     gene            5955..6740
                     /locus_tag="VNU01_11770"
     CDS             5955..6740
                     /locus_tag="VNU01_11770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWH33337.1"
                     /translation="MDDHADAPGAREMPAVPPVTDPAVLEEVRARRDEVPWRGADAFG
                     AALVYLLGGALLGVALATVGLLDPGSAPPPLLDSLLLLGACAGWLAVVRAPSAVRRLA
                     GPREPGRPVLRGVLLGVLVGVVWPFADTALYELLTLAGVEFPPIQEHIREWLDEPATR
                     AWVAVDIVLVTPLAEEALFRGLLFQGLVRRWGVRAAAVGSALLFGLAHVESLGADSVF
                     MVASTMLFGVAAAWLLWRYGTLLAPVAAHVATNAIATAGLLAS"
     gene            6870..8207
                     /locus_tag="VNU01_11775"
     CDS             6870..8207
                     /locus_tag="VNU01_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HWH33338.1"
                     /translation="MAAAETPSTAGPVPAPAGVPVTSADAVVVGAGPAGSAAAALLAQ
                     AGHDVVLIDKERFPRDKVCGDGLTPRAVKALRMLGLRDEAEGRAPGWHRQTGLRMYGG
                     GVVLELPWPQLQDYPGHSVTATRALFDHTLARNAVKQGAQLWEGTEVTGPRWRDASHS
                     RVSGVRWRRKDDDGEVVAEGEVHAPIVLACDGGSSRFAVSMGLHRIESRPMGVAVRAY
                     YRHARADLDVMEGFLELYRGNELLPGYGWIFPLDDAQEGTLLNVGWGLLNTSEHFRST
                     NYRRTLDEWVGGFPPEWGISKDTMVGRPASAGLPMGHNRKPPVYKGALLVGDAAGMVN
                     PFNGEGISYAIEAGAFAAEAADAALRRRGDAPLAAYERRVAQEWGGYYTLGRMFVRLI
                     GHPEIMRFCTVHGMPRRTVMQFVFRLMAHLVNRDSEDATDRVINGLSRMVPAA"
     gene            8307..8672
                     /gene="ndhC"
                     /locus_tag="VNU01_11780"
     CDS             8307..8672
                     /gene="ndhC"
                     /locus_tag="VNU01_11780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWH33339.1"
                     /translation="MLAEYLPIVVLFAIAAGFVVLSVVVSSRLGPSNPNPVKQAAYES
                     GILPEPETNVRASRFPVKFYLVAMLFIVFDVEAVFFFPFATVVRDLGWYGLGAMAIFV
                     ALLFESYYYVLRRGGLEWD"
     gene            8663..9307
                     /locus_tag="VNU01_11785"
     CDS             8663..9307
                     /locus_tag="VNU01_11785"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWH33340.1"
                     /translation="MGLETRLPDGILLTSVEKFLGWGRAGSLWPATFGLACCAIEMMA
                     TGAAHYDLARFGMEVFRASPRQADLMIVAGRVSQKMAPIVRNLYDQMPEPKWAIAMGV
                     CASSGGMFNNYAVVQGVDHIIPVDMYVPGCPPRPEMLMDGILKLHEKIRDEQLVPRPR
                     KGESMEDFRRRTGWQGPRPEDVAGSPDIAQPGQRHDLHQVLGARDAAGRPGGRS"
     gene            9304..9954
                     /locus_tag="VNU01_11790"
     CDS             9304..9954
                     /locus_tag="VNU01_11790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWH33341.1"
                     /translation="MTDNPRMQVPDVTAQERINQIEASVAIQLDGKLAAVRDRIAGAF
                     PSCEFSGFRGELTVTAPPDVLVDLLTFCKTDPDLSCELLSDVTGVHWPGGVHEHQGQE
                     TTGWPTYTIEQAGRIDVLYILRSVSRNHWFRVKVSLPDEHAAVPSAVPVYRSANVLER
                     EVFDLMGVRFEGHPRLERLFMPEDWEGHPLRKDYPLGGVEVQYKGATVPPPSERQY"
     gene            9964..11277
                     /locus_tag="VNU01_11795"
     CDS             9964..11277
                     /locus_tag="VNU01_11795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWH33342.1"
                     /translation="MSEITEQRRDLAAVPEFTVVGADWQELPDAVEDDEMIINMGPQH
                     PSTHGVLRLVLRLDGEEVLDCQPVIGYLHTGIEKNLEYRPWTQGVTFVTRMDYLAPLF
                     NELAYCLAVERLLGISDQVPERAQAIRVIMTELNRVSSHLVGLATTGMELGAISAMLY
                     GFREREYILDIFEFVTGLRMNHAYIRPGGVVVDVPDGFEQRTREVIALLRTRLGELHD
                     LLTQNPIWIERNSGIGVLTPEQCFDYGITGPVLRSAGVAHDLRKTQPYCGIDSYDFDV
                     VTHTDGDCYARYLVRLGEIEQSLRIVEQALDRLPGGPVMVADKKIAWPAQLALGPDGL
                     GNAKEHISKIMGQSMEALIHHFKLVTEGFVVPQGSAYAAVESPRGELAAHVTSDGTNK
                     PYRVHLRDPSFVHIGALPALAGGLYVADIIAAVASMDPVMGGVDR"
     gene            11327..>11529
                     /locus_tag="VNU01_11800"
     CDS             11327..>11529
                     /locus_tag="VNU01_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWH33343.1"
                     /translation="MISPELKDAAQQLVDRYPVGRSALLPLLHLVQSEEGHVSAEGVA
                     FCAQTLGLTKAEVAAVATFYTMY"
BASE COUNT         1393 a         4296 c         4368 g         1472 t
ORIGIN      
        1 gccgtgctgg cgcccggctc gcgcagcgcg ccgctggcga tggcgctgca cgccgacggg
       61 cgcgtgcgcc tgcacgtgcg cgtcgacgag cgctcggcgg cgttcctggc cctgggcctg
      121 gccaaggcga gcggccggcc cgccgcggtg ctgtgcacgt ccggcaccgc ggcggccaac
      181 ttccacccgg cggtcctgga ggcgcacgag tcccgggtgc cgctgctggt gctcaccgcc
      241 gatcggccgc cggagctgcg cgggaccggc gccaaccaga ccaccgacca ggtcaagctg
      301 tacgggcagg cggtgcgctg gttctgcgag gtcggcgtgc cggaggcgcg cgccgggatg
      361 gtggcctact ggcgcgcgac cgcgtgccgg gcgtgggcgg aggcggccgg ctcgccggcc
      421 ggcccggtgc acctgaacgt ggcgctgcgc gagccgctga cgcccgagcc cggcgacgcc
      481 gagttccccg agccgctgga cggccgcgcg gacggcgcgc cctgggtggc ggtggagtcg
      541 cgcaccacgg tgcccgacgc cgacctcgtg gaggacctgg ccgcccgcgc ggcctcggtg
      601 atgcgcggcg cgctggtggt gggagagacg tcggcggacc cctacgagct ggtggcgttc
      661 gccgaccggc tgggctggcc ggtcatcgcc gaggcgcact cgggggcgcg ccaggggccc
      721 agcgccgtcg cgatgtacca ccatctgctg tccgacccgt cgttcgcgga cgcgcatcgt
      781 cccgacttcg tggttcgtgt cggccgcaca gggttgtcgc gccccctgga ggcctggctc
      841 cgcaccgccg gctggcacac gctgatcgac cgtgacgccg cctggctgga cccgggccgg
      901 tccgtgtcgg agatcatcgt ggctgagccc gggcagttgc tgggcatggt cggcgccgcg
      961 ctcgcggact tggagagcga cgacggcgtg gaggactgga acgcgtcctg gggtgccgct
     1021 gatcgccggg ctcgcgacgc cgtgaaccag gccctggacg ccgagggcct gacggagccg
     1081 cgcgtggcgc gggacgtcgc cgcgtccctg ccgccgggag ccaccctggt ggtcggctcc
     1141 agcatgccga tccgcgacct cgaccagacc atggcgccgc gcggcgacgt gcgcgtgctg
     1201 gccaaccgcg gcgtcagcgg catcgacggg ttcgtctcca cggcggtggg cgtggcgctg
     1261 gcggccgggc cggcggtggc gctgtgcggc gacctgtcgc tgctgcacga cgcgaacggc
     1321 ctgctgggcg ccgacgccga cctcacgctg gtggtggtca acaacgacgg cggcggcatc
     1381 ttctcgttcc tgccgcaagc ccggcacccc gacggcttcg agcgcctgtt cggcacgccg
     1441 cacggcgccg acctcgaggc gctggcgcgc gcctacggcg ccgcctacac gcgcgtcacc
     1501 gcgcccgggg agctgcgcga cctcgtcgcg gcgccgcggg gcgtgcggct ggccgaggtg
     1561 cgcaccgacc gcgcggccaa cgtcgcgctg caccagcgcc tgcgcgacgc cgtgagccag
     1621 gcgctcgcgg gctagtccgc gaaggccgat tgcatcgcgc gcagcttgag gcgcgtctcc
     1681 tccagctccg cctcgggcag cgaggcggcg acgatgcccg cgcccgcgaa cagccgcgcc
     1741 cgcgccccgg acagctcagc gcagcgcagc gcgatcgcga actcgccgtc gccgcgcgcg
     1801 tccacccagc cgaccgggcc ggtgtagcgc ccgcgctcca tgccctccag ctcgcggatg
     1861 acctccagcg cctccgcggt gggcgtgccg cacacggcgg ccgtggggtg cagcgcgccg
     1921 gccagcgcca gcgcgctcgg cgccggctcg gccagcgtcc cgccgaaccg cgttgccaga
     1981 tgctgcacgt tgtccaggcg cagcagcgac ggctccgcgt ccgactccag cgtccggcac
     2041 agcggggcca gcgcctcgcg gaccgacgcc accgcgaagg cgtgctccgc gcggtccttg
     2101 gcggaggcga gcagcgcgtc gccgcgcgcg gcgtcgtcgg ccgcgccgtc gcctcgtgcc
     2161 gcggagccgg ccagcaccag cgactccacg cgggtgcccc gccggcgcac gagcatctcc
     2221 gggctcgcgc ccaccaggcc ctcggccacg aaggtgaagc agccggggaa gcgcgcggcc
     2281 aggcgggcgg cgagcacgcg cggctcgaac ggcgcctccg accacacggc gtggtcccgc
     2341 gccagcacca ccttgtcgat gtcgcccttg tcgatcaggc gcgtggcggc ggcgaccgcg
     2401 tccagccaca gcaggtccgg cagcgacgcg cccgcgtagc gcacgcggtc ggcggtggcg
     2461 cgctccggca gcaccgtcgc cgccagcggc gccaaggcgt ccggccgggc gtccggcccg
     2521 gcgccggccg cgaccgcgcg cgccgcggcg ccactgtgca ccagcagctc cggcacgcgc
     2581 acgaacgagc cgtgcgcggc ggggtcgaac gtgaaggcgg ccatggccac cggtgccgcg
     2641 ccgcgcccgt cccagccggc gaacgcctcc gccagcgcgg cgccggcgcg ggcgaagcgc
     2701 tccggccccg cgccgggatc cagccgccag gcctggcccc agccgacgaa gccggtgccg
     2761 tcgcgcagcc acgccagcgg ctctcccgcg cgctcgccgg gggacagggc caagagctcg
     2821 gccaccgtgc ccgcgcgcac gtcggggcga tcggcggtgc cgaagcgcag cgtcatggtg
     2881 cctgcgtctc ccggcgcagc cgctcgcgcg ccgcggccag cagcgtgcgg cggaagtggt
     2941 gggcgaccag gccgctggtg gcgggcagca tgcgctcgaa ggcgtcgcgc atgcggcgcc
     3001 cggcctcctc ggcgtcctcc gcggtgccgc ggatgggatc gcgcacgaag cgcacgaaca
     3061 gctccacggc ctcctccgcc acggcgcgca tggcttcgtc gtggcgccgc gccagagcca
     3121 gcagctcggc caggggcagc ccggcggcca gcagctccat gcccgcggcg agcacccgcg
     3181 cgtcgtcctc gccgtagccg tcctcgcccg gctggagcag gccctcgcgc tccatcgcct
     3241 ccaggagcga ctcgggcacg ccggcgcgct cggccagcgc cgcgcgggac aggcgctcgc
     3301 cgtcgcccga gtcgccgccg cgcagcaggg cggccagcgc ggcgtccgcg gagtccagct
     3361 cgggctgggg ggcgtcggcg gcgggagagg aggcggtcac ctcgccatgg tacggaaggt
     3421 gccgggacgg ggacgaaccg ctaccagtcg agctcgccgg agaagtccgt gtcgccggcg
     3481 tcggcgtcgg cgtcggcgtc gtcctccggt ccggcggccc cgccgtcctc gtcgggcgcg
     3541 cccgccgtgt ccggcccgtc ctcgtcggcc agcgcgagcc ccgccgcggc ggggccggcg
     3601 accggcgcca cggacacctc cggcgggcgc tcccgcggtg ggctgggctc ggcgtcgtgc
     3661 gcggtgtcca gcggctgcgt cgtctcggac tcggcgtgcg cgggctgcac gcggtgaccg
     3721 aggtcgcagc gcccgtcctc gcccaccggc accacctcgc cgcacagcgg gcaggccagc
     3781 gtgccgggac cggcgggcgc gccggccgcg gtgacgcgcg cgttcttgcg ccgcagcggc
     3841 atgggtcctc ctggtcgtcc aggccgtccg tcggcggcct cgcgcgggcg ggccgcctgt
     3901 cgccgacacc ctacactcgc gccgtgccgc cgatcctgta ctccgccgac gtggtgtgcc
     3961 cgatgagcgg gccgcccctg gcgcagggcg gcgtcctggt ggacggcgac cgcgtcgccg
     4021 cggtcggacc gtcggcgtcg ctgcgcggcg acgccgcccg ggagcaccgc atcccggggg
     4081 tgctgctgcc gggcatggtc aacgcccaca gccacctgga gtacgccgac gccgccgagc
     4141 tggcgcgacc gtgcccgttc gtgcagtggg tgctggacct gggccggctg gtccagggct
     4201 gggacgccga ccgctggtcg cgctcggcgc accgcggggc gcaggcgacg ctgcgcggcg
     4261 gcgccacggc ggtgtgcgac atcgtcacca agggcccggc cgtgcccgcg gccggcacgc
     4321 tcgggctggg cgggctgtcg tgggtggagc tggcgttcgt cgactgggag caccacgacg
     4381 acgtcctggc caagctgcgc gccgcgctgg agctgcccgc gcacggccgc gtggtggggc
     4441 tggcgccgca cgcgccgtac tccgtgggca ccggggtgct gcaggccgtc gcggcgctgg
     4501 cgcgcgagcg cggcgtgccg ctgcacaccc acgccgccga gacgcgcgag gaggtcgagg
     4561 cgctgcgcca ggggaccggt ccgctggcgc agctggcgcg gggtcgcggc atggacttcg
     4621 agtggctgga cggcgggacc ggcctgtcgc cggtgcgcta cctggacgcc tgcggcgccc
     4681 tgtttcccgg cgccagcgtg gcccacggcg tgtgggtcga cggcgaggag gccgccctgc
     4741 tggccgagcg cggcgcggcc gtggtgctgt gcccgcgctc caacgcgctg ctgtcggccg
     4801 gggacgcgcc gctggagcgc tacgccgagg cgggcacggc gctggcgctg ggcaccgaca
     4861 gcgcggcctc ctgccccgac ctggacgtgc tcggcgaggc gcacgcatgg gtggagctgg
     4921 cccgccgccg ggggctggac gcgtggccgg ccgacggcgg gccgcgcgag ctggccgagc
     4981 aggccatccg gctggcgacc gtggacggcg ccgccgccat ggggctcggc gccgccgccg
     5041 gcgtgatcga gccgggccgc cgcgcggacc tcgtgggtgt cgccgtggac acgaccgtgg
     5101 aggatgtgtg gcgcgacctc gtcgcccacg gcccgggcca ggccgtgtgc accgtggtcg
     5161 ccggcgtgcg caaggccagg cgcgagcccg gcaccccgtg gcccgagatc gaccgacagg
     5221 agtggcgagc atgagccccg accttcccga cccgcgcccc gggccgcaga agcaggccgc
     5281 gctggtgcag gcgatgttcg accgcgtggc gccgcgctac gacctggcga acacgctgct
     5341 gtccctgggg caggaccggc actggcgccg ggtcgccgcc cgggccgccg aggtgggccc
     5401 ggaggacgtg gtcgccgacg tcgccgccgg cacgggcgcg ctgtcgcggg agctccaggc
     5461 gcacggcgcc cgcgtcgtcg cgctggactt cagcctgaac atgctgcgcg ccggcgccac
     5521 tcgtgacgac accgccgacc ggatcgcgtg ggtcaacggg gacggcatgc gcctgcccct
     5581 gccggacgcc ggcgtggacg cggtcaccat cggcttcggc ttgcgcaacc tgcccgaccc
     5641 ccaggccggc ctggccgagt tcgcgcgcgt ggtccgcccg ggcgggcgcc tggtggtgct
     5701 ggagttcagc catccggtgt ggccgccgct gcgcgccgtc tacacgcgct acctggtcgg
     5761 ggcgctgccg cacgcggcca agctggtcac cagcgatccg gcggcctacc gctacctggc
     5821 ggagagcatc caggagtggc cgggccaggc cgagctcgcg gagctgatcg ccaaggcggg
     5881 ttggaccggc gtgcgctgga agaacctcac cgggggcatc gtcgcggtgc accaggccgt
     5941 tcggccctga cgccgtggac gaccacgcgg acgcaccggg cgcacgcgag atgcccgcgg
     6001 tgccgccggt gaccgacccg gcggtgctgg aggaggtgcg cgcgcggcgc gacgaggtgc
     6061 cgtggcgcgg cgccgacgcg ttcggcgcgg cgctggtcta cctgctcggc ggcgcgctgc
     6121 tcggcgtggc gctggcgacg gtggggctgc tggatcccgg gtccgcgccg ccgccgctgc
     6181 tggacagcct gctgctgctc ggcgcgtgcg ccggctggct ggcggtggtg cgggccccga
     6241 gcgccgtccg ccggctggcg ggcccgcgcg agccgggccg gccggtgctg cgcggcgtgc
     6301 tgctgggcgt gctcgtcggc gtggtgtggc cgttcgccga caccgcgctg tacgagctgc
     6361 tcacgctcgc gggcgtggag ttcccgccga tccaggagca catccgggag tggctggacg
     6421 agccggccac gcgcgcatgg gtcgccgtgg acatcgtgct ggtcacgccg ctggcggagg
     6481 aggcgctctt tcgcggcctg ctgttccagg gcctggtccg ccgctggggc gtgcgcgccg
     6541 ccgccgtcgg ttccgcgctg ctgttcggcc tggcgcacgt ggagtcgctg ggcgccgaca
     6601 gcgtgttcat ggtcgcctcg accatgctgt tcggtgtcgc ggcggcgtgg ctgctgtggc
     6661 gctacgggac gctgctcgcg ccggtcgcgg cgcacgtggc caccaacgcc attgcgacag
     6721 cgggtctgct cgcgtcctag gatcaccccg cggccgccgg gagttggcgg ctcgcgacac
     6781 aggctcgtac actcgggcac gccccttgtg aaagctttca caagacaccc cgcccgcccg
     6841 cttcctcccc gcgcgaagga gtgcgcaccg tggccgccgc agagacgccg agcaccgccg
     6901 ggcccgtccc ggcgcccgcc ggcgtgcccg tgaccagcgc cgacgccgtg gtggtcggcg
     6961 cgggccccgc gggctcggcc gccgccgcgc tgctggccca ggccggccac gacgtggtgc
     7021 tcatcgacaa ggagcggttc ccccgcgaca aggtctgcgg cgacggcctg acgccgcgcg
     7081 ccgtgaaggc gctgcgcatg ctgggcctgc gcgacgaggc ggagggccgc gcgccggggt
     7141 ggcaccgcca gaccggcctg cgcatgtacg gcggcggcgt ggtgctggag ctgccctggc
     7201 cgcagctgca ggactacccg ggtcactccg tcaccgcgac ccgcgcgctg ttcgaccaca
     7261 ccctggcgcg caacgccgtg aagcagggcg cccaactgtg ggagggcacg gaggtcaccg
     7321 ggccccgctg gcgcgacgcg tcgcacagcc gcgtgagcgg cgtgcgctgg cggcgcaagg
     7381 acgacgacgg cgaggtggtc gccgagggcg aggtgcacgc gcccatcgtg ctggcctgcg
     7441 acggcgggtc cagccgcttc gcggtgtcca tggggctgca ccgcatcgag tcgcgcccca
     7501 tgggcgtggc cgtgcgcgcc tactaccgcc acgcgcgcgc ggacctggac gtcatggagg
     7561 gtttcctgga gctgtaccgc ggcaacgagc tgctgccggg ctacggctgg atcttcccgc
     7621 tcgacgacgc ccaagaaggc accttgctga acgtcggctg gggcctgctg aacacttccg
     7681 agcacttccg ctccaccaac taccgcagga ccctggacga gtgggtcggc ggcttcccgc
     7741 cggagtgggg gatcagcaag gacaccatgg tgggccgtcc cgcgagcgcg ggcctgccca
     7801 tgggccacaa ccgcaagccg ccggtctaca agggcgcgct gctggtcggc gacgcggccg
     7861 gcatggtcaa ccccttcaac ggcgagggca tcagctacgc catcgaggcg ggcgcgttcg
     7921 ccgccgaggc cgcggacgcc gcgctgcgcc gccgcggcga cgcgccgctg gccgcctacg
     7981 agcgccgcgt ggcgcaggag tggggcgggt actacacgct gggccgcatg ttcgtgcggc
     8041 tcatcgggca cccggagatc atgcgcttct gcacggtcca cgggatgccg cgccgcacgg
     8101 tcatgcagtt cgtgttccgg ctcatggccc acctggtcaa ccgcgacagc gaggacgcca
     8161 ccgaccgggt gatcaacggg ctgtcgagga tggtgcccgc cgcatgacgc gcatcgaggt
     8221 ttgtcgccgc acgaggggcc gccctaggct cctgccgacg ccttgcgccg tgcccccgcg
     8281 tccacaccga ccagggagcg tccgccgtgc tcgctgagta cctgcccatc gtcgtgctgt
     8341 tcgccatcgc ggcgggcttc gtggtgctgt ccgtggtggt gtccagccgc ctcggcccca
     8401 gcaaccccaa cccggtgaag caggcggcct acgagtcggg catcctgccc gagccggaga
     8461 cgaacgtgcg cgccagccgc ttcccggtga agttctacct ggtggccatg ctgttcatcg
     8521 tcttcgacgt cgaggcggtg ttcttcttcc cgttcgccac ggtggtccgc gacctcggct
     8581 ggtacgggct gggcgcgatg gcgatcttcg tcgcgctgct gttcgagagc tactactacg
     8641 tcctgcgccg cggaggcctg gaatgggact agagacccgg ctgcccgacg gcatcctgct
     8701 cacctcggtc gagaagttcc tcggctgggg ccgggccggc tcgctgtggc cggcgacgtt
     8761 cggcctggcc tgctgcgcca tcgagatgat ggccacgggc gccgcgcact acgacctcgc
     8821 gcgcttcggc atggaggtgt tccgcgcctc gccgcgccag gccgacctca tgatcgtggc
     8881 gggccgcgtg agccagaaga tggcgccgat cgtgcgcaac ctctacgacc agatgccgga
     8941 gcccaagtgg gcgatcgcca tgggcgtgtg cgccagctcc ggcggcatgt tcaacaacta
     9001 cgccgtcgtg cagggcgtgg accacatcat ccccgtggac atgtacgtgc cgggctgccc
     9061 gccgcgcccg gagatgctga tggacggcat cctcaagctg cacgagaaga tccgcgacga
     9121 gcagctggtc ccgcgtccgc gcaagggcga gtcgatggag gacttccggc gccgcaccgg
     9181 ctggcagggc ccgcgcccgg aggacgtcgc cggctcgccg gacatcgccc agccgggcca
     9241 gcgccacgac ctgcaccagg tcctgggcgc gcgcgacgcc gccggccggc cgggagggcg
     9301 gtcatgaccg acaacccgcg catgcaggtc ccggacgtca cggcccagga gcgcatcaac
     9361 cagatcgagg cgtcggtcgc cattcagctc gacggcaagc tggcggcggt gcgcgaccgc
     9421 atcgcgggcg cgttcccgtc ctgcgagttc tccggtttcc gcggcgagct caccgtcacg
     9481 gccccgccag acgtgctggt ggacctgctg acgttctgca agaccgaccc ggacctgtcc
     9541 tgcgagctgc tgagcgacgt cacgggcgtg cactggcccg gcggtgtgca cgagcaccag
     9601 ggccaggaga ccaccggctg gcccacctac acgatcgagc aggccgggcg catcgacgtc
     9661 ctgtacatcc tgcggtcggt gagccggaac cactggttcc gcgtgaaggt gtcactgccg
     9721 gacgagcacg cggccgtgcc cagcgccgtg ccggtgtacc gctcggcgaa cgtgctggag
     9781 cgcgaggtct tcgacctcat gggcgtgcgc ttcgagggcc acccgcgcct ggagcggctg
     9841 ttcatgccgg aggactggga gggccatccg ctgcgcaagg actacccgct ggggggcgtg
     9901 gaggtccagt acaagggcgc gaccgtgcct ccgccgagcg agcgccagta ctaggggtcg
     9961 atcatgagcg agatcaccga gcagaggcgc gacctggccg ccgtccccga gttcaccgtc
    10021 gtgggcgcgg actggcagga gctgccggac gccgtcgaag acgacgagat gatcatcaac
    10081 atggggccgc agcacccgtc gacccacggg gtgctgcgcc tggtgctgcg cctggacggc
    10141 gaggaggtgc tggactgcca gccggtcatc ggctacctgc acaccggcat cgagaagaac
    10201 ctcgagtacc gcccctggac gcagggcgtc acgttcgtga cgcgcatgga ctacctggcg
    10261 ccgctgttca acgagctggc gtactgcctg gccgtggagc gcctgctggg catctccgac
    10321 caggtgccgg agcgcgccca ggcgatccgc gtgatcatga cggagctcaa ccgcgtctcc
    10381 agccacctgg tcggcctggc caccaccggc atggagctcg gcgccatctc cgcgatgctg
    10441 tacggcttcc gcgagcgcga gtacatcctc gacatcttcg agttcgtcac cggcctgcgc
    10501 atgaaccacg cctacatccg cccgggcggc gtggtggtgg acgtgccgga cgggttcgag
    10561 cagcgcacgc gcgaggtcat cgccctgctg cgcacgcgcc tgggggagct gcacgacctg
    10621 ctcacccaga accccatctg gatcgagcgc aactccggca tcggcgtgct caccccggag
    10681 cagtgcttcg actacggcat caccggcccg gtgctgcgca gcgccggcgt cgcgcacgac
    10741 ctgcgcaaga cgcagcccta ctgcggcatc gactcctacg acttcgacgt ggtgacccac
    10801 accgacggcg actgctacgc gcgctacctg gtgcgcctgg gcgagatcga gcagagcctg
    10861 cgcatcgtgg agcaggcgct ggaccgcctg cccggcgggc ccgtcatggt ggccgacaag
    10921 aagatcgcct ggccggcgca gctggccctg gggccggacg ggctcggcaa cgccaaggag
    10981 cacatctcca agatcatggg ccagtccatg gaggcgctga tccaccactt caagctggtg
    11041 acggagggct tcgtggtgcc gcagggcagc gcgtacgcgg ccgtcgagtc gccgcggggc
    11101 gagctcgccg cgcacgtcac gtccgacggc accaacaagc cctaccgcgt gcacctgcgc
    11161 gaccccagct tcgtgcacat cggcgccctg ccggccctgg ccggcggcct gtacgtcgcg
    11221 gacatcatcg ccgccgtggc cagcatggac cccgtgatgg gcggggtgga ccgctgagcc
    11281 gcccacgcgc actccccgaa ccccgaacga cctggagcag ccagacgtga tctcgccaga
    11341 gctgaaggac gcggcacagc agctggtcga ccggtacccg gtcggccgct ccgcgctgct
    11401 gccgctgctg cacctggtgc agagcgagga gggccacgtg tccgcggagg gcgtcgcctt
    11461 ctgcgcgcag acgctcgggc tcaccaaggc ggaggtcgcg gcggtggcga ccttctacac
    11521 catgtacaa
//
LOCUS       DATMKC010000405         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_53105, whole genome shotgun sequence.
ACCESSION   DATMKC010000405 DATMKC010000000
VERSION     DATMKC010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53105"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..995
                     /locus_tag="VNU01_11805"
     CDS             42..995
                     /locus_tag="VNU01_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWH33344.1"
                     /translation="MQLLRAPSPPTATFPIRTVPKGPWAYLRIAGGCDRVCTFCAIPS
                     FRGRFVSRPMDELLAEARWLADGGARELVCVSENTTSWGKDLPDGRHGIVKLIRGFEQ
                     VDGLERVRLMYLQPAEIIPELLDAMVASPVVAPYYDLSLQHASEPVLNGMARSGSPER
                     FLALIEGIRRRDPAAVFRSSFILGFPGETERDVEVLAEFLRQARLDWAGFFTFSVEEG
                     TPSATMPGQVPHEEAAARRDAMVELYEALAEDNTAGFVGRTLDVLIEEQDGPDAVGRS
                     YREAPETDGEVRLPGCEIPLGRMLPVRITGADGVDLIGVPA"
     gene            992..1528
                     /gene="pgsA"
                     /locus_tag="VNU01_11810"
     CDS             992..1528
                     /gene="pgsA"
                     /locus_tag="VNU01_11810"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWH33345.1"
                     /translation="MTEAEPRWLNWPNAFTLLRVALVPVIALLLFADTEAARWWAFAV
                     FVFAALTDTVDGWLARRYVGVTRWGQLADPAADKALIVGALAVLAARGELPWWAVGVI
                     VLREVAVTVQRSVLVRRGVVMPASALGKVKTVTQVVAVTLYLLPAAPAALADVALALA
                     VLVTVVSGLEYALRGGRR"
     gene            1525..>2745
                     /locus_tag="VNU01_11815"
     CDS             1525..>2745
                     /locus_tag="VNU01_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HWH33346.1"
                     /translation="MRAEVVAVGSELLMGEGVDTNSAWISARLAEIGVDVHRHTTVGD
                     NLERLTAVLREAAERADAVVVTGGLGPTQDDLTRFAVARVAGVELERREDLAQWLRDY
                     FARAGREMPERNLVQADIPAGARVLEAAGTAAGFVVELPGSVVYCVPGPPSEMKILME
                     RDVLPDLARRGGLAVTLSRTVHTAGIGEAAVAEACAGLVERLDREGGVTLAFYASGGE
                     TRVQLRAKASTRQEAEALLAPVVEEVVALLGPGFAGLDGEGVEHAIARLLREAGLTLG
                     LAESVTGGGVGARLVRVAGASDWLRGGLVVYATDAKRDLAGVDAGLLERHGPVSEPIA
                     AALARAAAERLGADVGLAVVGVAGPATQGDQPVGTVCLGFAFPAMGVERTRTLRTPAR
                     ERGEALEFAASAALD"
BASE COUNT          310 a          974 c         1090 g          371 t
ORIGIN      
        1 gtgtgcagtt gctccgggat atcggaggaa ctgcacacag tgtgcagttg ctgcgggcgc
       61 cgtcgccgcc gaccgccacg ttcccgatcc gcaccgtgcc caagggcccg tgggcgtacc
      121 tgcgcatcgc gggcggctgc gaccgtgtct gcacgttctg cgcgatcccc agcttccgag
      181 gccgcttcgt ctcccggccc atggacgagc tgctcgccga ggcgcgctgg ctcgccgacg
      241 gcggcgcccg cgagctggtc tgcgtcagcg agaacaccac gagctggggc aaggacctgc
      301 ccgacggccg tcacggcatc gtcaagctga tccgcggctt cgagcaggtc gacggcctgg
      361 agcgcgtccg gctcatgtac ctgcagcccg cggagatcat cccggagctg ctcgacgcga
      421 tggtcgcctc gccggtggtg gcgccgtact acgacctgtc cctgcagcac gccagcgagc
      481 cggtcttgaa cggcatggcg cgctcgggca gcccggagcg cttcctggcg ctcatcgagg
      541 gcatccgccg ccgcgacccc gccgcggtgt tccgcagctc gttcatcctg ggcttcccgg
      601 gcgagaccga gcgcgacgtc gaggtgctgg cggagttcct tcggcaggcg cggctggact
      661 gggcgggctt cttcacgttc tccgtggagg agggcacgcc gtcggcgacc atgcccggcc
      721 aggtgcccca cgaggaggcc gccgcccggc gcgacgcgat ggtcgagctg tacgaggcgc
      781 tggcggagga caacacggcc ggcttcgtcg ggcgcaccct ggacgtgctg atcgaggagc
      841 aggacggccc ggacgccgtc gggcgcagct accgcgaggc gccggagacc gacggcgagg
      901 tgcgcctgcc cggctgcgag atcccgctcg gccgcatgct gccggtgcgg atcacgggcg
      961 ccgacggcgt ggacctgatc ggcgtgcccg catgaccgag gcggagccgc gctggctgaa
     1021 ctggccgaac gcgttcacgc tcctgcgggt cgcgctggtc ccggtcatcg cgctgctgct
     1081 gttcgccgac accgaggcgg cccgctggtg ggccttcgcc gtgttcgtgt tcgccgcgct
     1141 caccgacacc gtggacggct ggctcgcccg ccgctacgtg ggcgtgaccc gctgggggca
     1201 gctcgccgac ccggccgccg acaaggcgct catcgtcggc gccctggcgg tgctggccgc
     1261 caggggcgag ctgccgtggt gggcggtcgg cgtcatcgtg ctgcgcgagg tcgcggtcac
     1321 cgtgcagcgc agcgtgctgg tgcgccgcgg cgtcgtcatg ccggccagcg cgctcggcaa
     1381 ggtcaagacg gtcacgcagg tggtcgccgt cacgctgtac ctgctcccgg cggcgccggc
     1441 cgcgctggcg gatgtggcgc tggcgctcgc cgtgctggtg accgtcgtca gcggcctgga
     1501 gtacgccctg cgcggaggcc gccggtgagg gcggaggtcg tcgccgtcgg ctccgagctg
     1561 ctgatgggcg agggggtcga caccaacagc gcgtggatct ccgcgcggct ggccgagatc
     1621 ggcgtggacg tgcaccggca caccacggtg ggggacaacc tggagcggct gaccgccgtg
     1681 ctgcgcgagg cggccgagcg cgccgacgcc gtcgtcgtca ccggcggcct ggggcccacg
     1741 caggacgacc tgacgcgctt tgcggtcgca agggtggcgg gggtggagct ggagcggcgc
     1801 gaggacctgg cgcagtggct gcgcgactac ttcgcgcgcg cgggccgtga gatgccggaa
     1861 cgaaacctcg tgcaggccga catccccgcc ggcgcccgcg tcctggaggc ggcgggcacg
     1921 gccgccggct tcgtggtgga gctgcccggt tccgtcgtct actgcgtgcc gggcccgccg
     1981 agcgagatga agatcctcat ggagcgcgac gtgctgccgg acctggcgcg ccgcggcggc
     2041 ctggcggtca cgctgtcgcg cacggtgcac accgccggca tcggcgaggc cgcggtggcc
     2101 gaggcctgcg ccgggctggt cgagcgcctg gaccgcgagg gcggcgtgac gctggccttc
     2161 tacgcctcgg gcggcgagac gcgcgtgcag cttcgggcga aggcgtccac gcggcaggag
     2221 gccgaggcgc tgctcgcgcc cgtggtcgag gaagtggtcg cgctgctcgg cccgggcttc
     2281 gcggggctgg acggagaggg cgtcgagcac gcgatcgcgc gcctgctgcg ggaggccggc
     2341 ctgacgctgg ggctggcgga gtcggtgacc ggcggcggcg tgggcgcccg cctggtgcgc
     2401 gtcgccgggg ccagcgactg gctgcgcggc ggcctggtgg tctacgccac ggacgccaag
     2461 cgcgacctcg cgggggtgga cgcgggactg ctggaacgcc acggcccggt gagtgagccg
     2521 atcgccgcgg cgctggcgcg cgccgcggcc gagcgcctcg gcgccgacgt cggcctggcc
     2581 gtggtcggcg tcgccggccc cgccacccag ggcgaccagc cggtcgggac cgtctgcctg
     2641 ggcttcgcgt tcccggccat gggcgtcgag cgcacgcgca cgctgcgcac cccggcccgc
     2701 gagcgcggcg aggcgctgga attcgccgcc agcgcagccc tggac
//
LOCUS       DATMKC010000406         2503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_249144, whole genome shotgun sequence.
ACCESSION   DATMKC010000406 DATMKC010000000
VERSION     DATMKC010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249144"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VNU01_11820"
     CDS             complement(<1..569)
                     /locus_tag="VNU01_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33347.1"
                     /translation="MAEALREVEPSPAPQRWRWFDRPLRRDALFWYSLAMIPLGPLCW
                     AGLAGVPIEVRRARRRRAAGLRGFESRRTPWILGCGALIGVVANVAFVFGVVQQRADA
                     AGARAAATACVTVFSAFADGAPSDSSVDAAFRQSDGLTARAAAHDPRWQPLRDRLLAL
                     DSAWWTGSDAEYQDAAIAFDDWCRGAFGGT"
     gene            complement(630..1256)
                     /locus_tag="VNU01_11825"
     CDS             complement(630..1256)
                     /locus_tag="VNU01_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH33348.1"
                     /translation="MEPRAVVGRAGEPRTRLSAQERRQQLLEVAGGLFAEHGFHGLSM
                     EQLADAAGVSKPVLYQHFPSKRELYLALMQDAIAEMESQIRKALEGTRDNRARVEGAI
                     AAYFDFVEDRRSRLIFGAYEAADPDARDAVEEANRRTAEAVGQLIAEDAGLDRAAAGL
                     LATALRGLATEAARWWTDHDADAGLDKAKAVRFLSRLAWRGLGSFQKD"
     gene            1411..>2503
                     /locus_tag="VNU01_11830"
     CDS             1411..>2503
                     /locus_tag="VNU01_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH33349.1"
                     /translation="MTFSLALSDEQQSAQQWAHDFAEKEIRPVASQYDETEDFPWEVV
                     KKAAQIGLYGLDYYQMSGQDQTGLTANLVVEEVFWGCAGIGLAIFGSGLCLAGLASTG
                     TMEQIGHWAPRIFGTPEDPKVGAYAVSEAGAGSDVRSLRTRAKKVDGGWVLNGQKSWI
                     TNGGIADVHVVVATVDPELGHRGQASFIVTSDMDGFSMAKKERKLGIRASHTAEIAFD
                     DVFVPDEHLLGGQDKLDEKIAKAKDPAAGKTKSGALSTFEATRPIVGIQAVGIARAAF
                     EFAAKYATERETFGVPIVKHQAIAFKLADMATEIDAARLLCWRGLWMGINRVPFKYGE
                     GSMAKLKAGRVAVWSTDEAIQILGGYGYVK"
BASE COUNT          376 a          906 c          881 g          340 t
ORIGIN      
        1 gtcccgccga aagcaccccg gcaccaatca tcgaacgcga tcgccgcatc ctggtactcg
       61 gcgtcgctgc ccgtccacca ggcggagtcg agggccagca ggcggtcacg cagcggctgc
      121 cagcgcggat cgtgcgccgc cgcgcgtgcg gtgagcccgt cggactggcg gaaggcagcg
      181 tccacggagc tgtcgctcgg cgcgccgtcg gcaaacgccg agaacaccgt gacgcacgcc
      241 gtcgcggccg cgcgtgcgcc cgcggcgtcg gcccgctgct gcacgacgcc gaagacgaag
      301 gccacgttcg ccacgacgcc gatcaacgcg ccacagccca agatccaagg ggtgcgccgt
      361 gactcgaagc cgcgcagccc ggcggcgcgg cggcggcggg cgcgccgcac ctcgatcggc
      421 actcccgcca gccccgccca gcacagcggt cccaagggga tcatcgcgag gctgtaccag
      481 aacagcgcgt ctcgtcgcag cggccgatcg aaccagcgcc atcgctgtgg cgcgggtgag
      541 ggctcgactt ccctgagcgc ctccgccatg tcttcccccg ttcgtctgcc acagcgtcgg
      601 cagtcccgtt cgggggcttg agaggcgggt cagtccttct ggaacgagcc gaggccgcgc
      661 caggccaggc gggacaggaa gcgcacggcc ttggccttgt ccagaccggc gtcggcgtcg
      721 tggtcggtcc accagcgcgc cgcctcggtc gccaggccgc gcagcgccgt ggccagcagt
      781 cccgcggcgg cgcggtccag ccccgcgtcc tcggcgatga gctggccgac ggcctcggcg
      841 gtgcgccggt tggcctcctc caccgcgtcg cgcgcgtcgg ggtcggcggc ctcgtaggcg
      901 ccgaagatca gccgggaccg gcggtcctcc acgaagtcga agtaggccgc gatggcgccc
      961 tcgacccgcg cgcggttgtc ccgcgtgcct tccagcgcct tgcggatctg cgactccatc
     1021 tccgcgatgg cgtcctgcat cagcgccagg tacagctccc gcttggacgg gaagtgctgg
     1081 tacagcaccg gcttgctgac accggccgcg tccgcgagct gctccatgga caggccgtgg
     1141 aagccgtgct cggcgaacag cccgccggcg acctccagca gctgctgccg gcgctcctgg
     1201 gcggacagtc gtgttcgcgg ctcgcctgct cggcccacca ccgctcgggg ctccacgttt
     1261 cctcctgctc gcgcgtcgat tcgaccggcc ggcggcctga accgtatgtt accgcctggt
     1321 aacctgctcg tgggtcaggg ggcatacccc gcccgtacga ctccgcacac cggcaccgga
     1381 cccgcgcggt cctcgatcga ggtggaagcc atgaccttca gcctggcgct ctccgatgag
     1441 cagcagtcgg cgcagcagtg ggcgcacgac ttcgcggaga aggagatccg ccccgtcgcg
     1501 tcccagtacg acgagaccga ggacttcccc tgggaggtcg tgaagaaggc cgcgcagatc
     1561 ggcctgtacg ggctggacta ctaccagatg agcggccagg accagaccgg cctgaccgcc
     1621 aaccttgtgg tcgaggaggt gttctggggc tgcgccggca tcggcctggc gatcttcggt
     1681 tccggcctgt gcctggccgg cctggcctcc acggggacca tggagcagat cggccattgg
     1741 gcgccgcgca tcttcggcac gccggaggac cccaaggtcg gcgcgtacgc cgtcagcgag
     1801 gcgggcgcgg gctcggacgt gcgctcgctg cgcacccgcg ccaagaaggt ggacggcggc
     1861 tgggtgctca acggccagaa gagctggatc accaacggcg gcatcgccga cgtgcacgtg
     1921 gtggtcgcca cggtcgaccc cgagctcggc caccgcggcc aggcgtcgtt catcgtcacc
     1981 agcgacatgg acggcttctc gatggccaag aaggagcgca agctcggcat ccgggccagc
     2041 cacacggccg agatcgcgtt cgacgacgtg ttcgtgccgg acgagcacct gctgggcggc
     2101 caggacaagc tggacgagaa gatcgccaag gccaaggacc cggccgccgg caagaccaag
     2161 tccggcgcgc tgagcacgtt cgaggccacc cgcccgatcg tgggcatcca ggccgtcggc
     2221 atcgcgcgcg cggcgttcga gttcgccgcc aagtacgcca cggagcgcga gacgttcggc
     2281 gtgccgatcg tcaagcacca ggccatcgcg ttcaagctcg ccgacatggc cacggagatc
     2341 gacgccgccc gcctgctgtg ctggcgcggc ctgtggatgg gcatcaatcg cgtgccgttc
     2401 aagtacggcg agggctcgat ggccaagctc aaggccggcc gggtcgcggt gtggtcgacc
     2461 gacgaggcga tccagatcct gggcggctac ggctacgtca agg
//
LOCUS       DATMKC010000407         8462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_165120, whole genome shotgun sequence.
ACCESSION   DATMKC010000407 DATMKC010000000
VERSION     DATMKC010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165120"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="miaA"
                     /locus_tag="VNU01_11835"
     CDS             <1..789
                     /gene="miaA"
                     /locus_tag="VNU01_11835"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWH33350.1"
                     /translation="YRGMDIGTAKPGARERERVPHYMVDVLEPEQDCSVRWFQQAARD
                     AIADVHRRGAVPLLVGGSGLYFRGVVDPLEFPPTDPAVRAGVEARHPTASSAHAALRE
                     ADPDAAAAMDPANRRRAVRALEVIELTGRPFSSFRTAWEHHDSVYPALRVLGADVPRQ
                     ELVARIDARTDAMLAAGWLEEARALRDRALSSTARQALGYAELFDHLDGRCTLDEAAE
                     RIRARTRRYAAYQARWFCADPRVAWRAPSELVDDLTRELAACAS"
     gene            777..1631
                     /gene="dapF"
                     /locus_tag="VNU01_11840"
     CDS             777..1631
                     /gene="dapF"
                     /locus_tag="VNU01_11840"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWH33351.1"
                     /translation="MRFVKAHGTGNDFVLLPDLDGALELSAGLVQALCAPHLGLGADG
                     VIRIAPPDPDLAAGQDADVFMDYRNADGGAVEMCGNGVRCVAKYVLDRGMVAGDTVRV
                     LTRSGVKTVQAARGADGKVVRVRVDMGAPSPGGVNQPLDVRLDGVEQIVRITTLSMGN
                     PHAVLVVDDVDAAPVRTLGPVIEGHPAFPDRTNVEFIAVPSRDRVEGRIWERGVGETL
                     ASGTGSSAMAVAAHLLGLADRRVTVALPGGEIAVEWTDDTLFMEGPAVEVAEGELGAD
                     WLAAVEDA"
     gene            1628..3028
                     /gene="hflX"
                     /locus_tag="VNU01_11845"
     CDS             1628..3028
                     /gene="hflX"
                     /locus_tag="VNU01_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWH33352.1"
                     /translation="MTGEDVDPDGGLSVTRDERAIGRAERRRLEADRVTEGGPREGVA
                     IFRPAERAVLVALHTQGTTSHEVAASLDELELLADTAGAASVGRVVQRRGEPDVATFI
                     GRGKVDELKDLARSLDADAVIFDDELSPAQQRNLEEKLGVIVLDRTIVILNIFADHAT
                     SREGKAQVELAQYGYMLPRLRGWGKALSRIGGGAGPMRGPGETQLEVDRRKIMRRITK
                     LRRDLKDFERTRQVKAQERGRRGVPVVALVGYTNAGKSTLMNQLTGADVLVADQLFAT
                     LDTTARRLSLPDQRDIVLMDTVGFVKKLPTQLVEAFKSTLEEALRADLLLHVVDGSHP
                     EAEAQILAVDRVLAEIGADRLPRLLVLNKADAADPDELRGLERTFPGSLAVSARTGAG
                     VAALLQRVSELLPQSRRVLEAVVPYERSDLVARAHREGEVLKEEHRPEGTYLVANLER
                     DLAEALRAYAQNDPFA"
     gene            complement(3347..3973)
                     /gene="lexA"
                     /locus_tag="VNU01_11850"
     CDS             complement(3347..3973)
                     /gene="lexA"
                     /locus_tag="VNU01_11850"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWH33353.1"
                     /translation="MALTERQQRILEMIRESVATRGYPPSVREIGEAVGLRSPSSVHS
                     QLAALERGGYLRRDPSRPRAIEVRFDPDTELSLPVVRSKQVPLLGEIAAGAPIIAEER
                     VEQVFPLPQELVGEGTLFMLRVRGESMVQAGVLPGDYVVIRQQPTVEQGDMCAALVDG
                     EATVKFFRRTKAGEVFLDPANDGYEPIPVTADQDSSILGKVVAVLRSV"
     gene            4211..4537
                     /locus_tag="VNU01_11855"
     CDS             4211..4537
                     /locus_tag="VNU01_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33354.1"
                     /translation="MATVQQVGYRRRPTRAAYWRRRAVALVVLVLALAGALTLLAPNS
                     GAAPAREPEPLLLVVGPGDTVLDLVRPFAPEGSDPRAFAAEVLEASDLDGRAVRPGAV
                     LRLPRS"
     gene            complement(4561..5088)
                     /gene="nrdR"
                     /locus_tag="VNU01_11860"
     CDS             complement(4561..5088)
                     /gene="nrdR"
                     /locus_tag="VNU01_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWH33355.1"
                     /translation="MRCPYCAVDDDKVVDSRPSEEGEAIRRRRECLSCGQRFSTYERV
                     EQLSLAVRKRNGLIEPFRRDKIEAGMAKATKNLDIEPADVARAVAQVEARVRAMGRRV
                     VPSEAVGAEVLEALRVLDHVGYMRFASVYKGFTSPEDFRRELASLEKDAPPKRPAGAP
                     PEPPSRPAGARREAR"
     gene            5471..8245
                     /locus_tag="VNU01_11865"
     CDS             5471..8245
                     /locus_tag="VNU01_11865"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWH33356.1"
                     /translation="MVTTRDALQAVPGKTGRAGLKFTRHFTTPGVHPYDELEWEVRDA
                     VITDFKTGAAAFEQRDVEFPKSWSMNATTIVAQKYFRGQLGTPARERSVRQMVDRVAD
                     TITAWGVKDGYFADEDSAEIFNAELKHLLVTQKAAFNSPVWFNCGVEEDPQCSACFIL
                     SVEDTMSSILNWYVEEGTIFKGGSGSGVNLSRIRSSAEKLKGGGEASGPVSFMRGADA
                     SAGTIKSGGKTRRAAKMVILNVDHPDIEEFIWCKQREEVKARALRELGFDMDLDGKDA
                     ASIQYQNANNSVRVTDEFMRAVENDDDFDLRAVTNGDVVRTVRARDLFRQIAQAAWEC
                     ADPGVQYDGTINDWHTTPNAGRINGSNPCSEYMHLDNSACNLASLNLRKFEYDGVFDV
                     DAFRRAVEVVFTAQEIIVGNSSYPTERIGANARAYRQLGIGYANLGGLLMSQGLAYDS
                     DEGRAWAAAITALMTGHAYRTSAEIAKVQGPFDGFAQDREGVLRVIAKHGQACEDIDA
                     ALAPDNILEAAKQSWADALMLGTEYGIRNAQASVLAPTGTIGLMMDCDTTGIEPDLGL
                     VKAKKLVGGGNMRIVNQTVPVALQKLGYQQEQIEAIVAYIDEHGTIEGAPAFRDEHLT
                     VFDCAMGTRSISPMGHVKMMAAVQPFISGAISKTVNLPETATVEEVEQLHLDAWKLGV
                     KAIAIYRDNCKVAQPLALAGSKNEPKKAGTTEAEAAQAGVYRRRLPKSRPSQTISFAV
                     GDADGYITAGEYPGDGLGEIFVKLGKQGSTLSGVMDAFAISVSLGLQYGVPLEAYVKK
                     FINMRFEPAGMTDDPEVKFASSIVDYMFRRLAIDYLPAEKRHELGIHTLEERNAMLDA
                     QYGGGGAAPADAAPVVDAEGQTVLPIEKPKPVDDVHGDAPMCFQCGVTMNRAGSCHVC
                     PQCGTTSGCS"
     gene            complement(8268..>8462)
                     /locus_tag="VNU01_11870"
     CDS             complement(8268..>8462)
                     /locus_tag="VNU01_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33357.1"
                     /translation="GRRFLTIAHPAFPLSSAGRGDGKETTADAGRDFHRAAVQTLKGG
                     TFGGVPPFTGLGVSSQSFSG"
BASE COUNT         1267 a         2988 c         3068 g         1139 t
ORIGIN      
        1 taccgcggca tggacatcgg cacggcaaag cccggcgccc gtgagcgcga gcgcgtgccc
       61 cactacatgg tcgacgtgct ggagccggag caggactgct ccgtgcgctg gttccagcag
      121 gccgcccggg acgccatcgc ggacgtgcac cgccgcggcg ccgtgccgct gctggtcggc
      181 ggctccggcc tgtacttccg cggcgtcgtg gacccgctgg agttcccgcc caccgacccg
      241 gccgtccgcg ccggcgtgga ggcgcgccac cccaccgcct cctccgcgca cgcggcgctg
      301 cgcgaggcgg acccggacgc ggcggcggcg atggaccccg ccaaccgccg gcgcgcggtg
      361 cgggcgctgg aggtgatcga gctgaccggc cgcccgttca gctccttccg cacggcctgg
      421 gagcaccacg actccgtgta cccggccctg cgcgtgctcg gcgccgacgt gccccggcag
      481 gagctggtcg cccgcatcga cgcgcgcacc gacgcgatgc tggcggccgg ctggctggag
      541 gaggcgcggg cgctgcggga ccgggcgctg tcgtccacgg cccgccaggc gctcggctac
      601 gcggagctgt tcgaccacct ggacgggcgc tgcacgctgg acgaggcggc cgagcgcatc
      661 cgcgcgcgca cgcgccgcta cgccgcctac caggcgcggt ggttctgcgc ggacccgcgc
      721 gtagcatggc gggcaccgtc ggaactcgtc gacgacctca cccgggagct ggccgcatgc
      781 gcttcgtgaa ggcccacggc acgggcaacg acttcgtgct gctgccggac ctcgatggcg
      841 cgctggagct ctccgccggc ctggtgcagg cgctgtgcgc cccgcacctg ggtctgggcg
      901 ccgacggcgt catccgcatc gcgccgcccg acccggacct ggcagccggg caggacgcgg
      961 acgtgttcat ggactaccgc aacgccgacg gcggtgcggt ggagatgtgc ggcaacggcg
     1021 tgcgctgcgt ggccaagtac gtgctggacc gcggcatggt ggccggcgac accgtgcgcg
     1081 tgctcacgcg cagcggcgtc aagacggtgc aggccgcgcg cggcgcggac ggcaaggtcg
     1141 tgcgcgtgcg ggtggacatg ggcgccccgt cgccgggggg agtgaaccag ccgctggacg
     1201 tgcgcctgga cggtgtcgag cagatcgtgc gcatcaccac gctgtcgatg ggcaatcccc
     1261 atgccgtgct ggtcgtcgac gacgtggacg ccgcccccgt gcgcaccctc ggccccgtga
     1321 tcgagggcca tccggcgttc ccggaccgca ccaacgtgga gttcatcgcc gtgccgtcac
     1381 gcgaccgcgt cgagggccgc atctgggagc gcggcgtcgg ggagacgctg gcgtcgggga
     1441 ccggctcgtc ggccatggcg gtggccgcgc acttgctggg cctggcggac cggcgcgtca
     1501 cggtggcgct gcccggcggc gagatcgccg tggagtggac cgacgacacg ctgttcatgg
     1561 agggtcccgc ggtcgaggtg gccgagggcg agctcggcgc cgactggctc gccgcggtgg
     1621 aggacgcgtg accggcgagg acgtcgaccc ggacggcggc ctgtcggtca cccgcgacga
     1681 gcgtgcgatc ggccgcgccg agcggcggcg cctggaggcc gaccgcgtca cggagggcgg
     1741 gccgcgcgag ggtgtcgcga tcttccgccc ggccgagcgg gccgtcctgg tggcgctgca
     1801 cacgcagggg accaccagcc acgaggtggc ggcctcgctg gacgagttgg agctgctggc
     1861 cgacaccgcc ggcgccgcgt cggtgggccg cgtggtgcag cgccgcggcg agccggacgt
     1921 cgccacgttc atcggccgtg gcaaggtcga cgagctgaag gacctggcgc gcagcctcga
     1981 cgccgacgcg gtgatcttcg acgacgagct gtcaccggcc cagcagcgca acctggagga
     2041 gaagctgggc gtgatcgtgc tggaccgcac gatcgtcatc ctcaacatct tcgccgacca
     2101 cgccaccagc cgggagggca aggcgcaggt cgaactggcc cagtacggct acatgctccc
     2161 caggctgcgc ggctggggca aggcgctgtc gcgcatcggc ggcggcgcgg gccccatgcg
     2221 cggccccggt gagacgcagc tggaggtcga ccgccgcaag atcatgcgcc ggatcaccaa
     2281 gctgcgccgc gacctcaagg acttcgagcg cacccgccag gtgaaggcgc aggagcgcgg
     2341 ccggcggggg gtgcccgtgg tcgccctggt cggctacacc aacgcgggca agtccacgct
     2401 gatgaaccag ttgaccggcg ccgacgtgct ggtggccgac cagctgttcg cgacgctgga
     2461 caccacggcg cgccgcctga gcctgccgga tcagcgcgac atcgtgctca tggacacggt
     2521 gggcttcgtg aagaagctgc ccacgcagct ggtggaggcg ttcaagtcga cgctggagga
     2581 ggcgctgcgg gcggacctgc tgctgcacgt ggtggacggc agccacccgg aggccgaggc
     2641 gcagatcctg gccgtggacc gcgtgctcgc cgagatcggc gccgaccgcc ttccgcgcct
     2701 gctggtgctg aacaaggccg acgccgcgga ccccgacgag ctgcggggcc tggagcggac
     2761 gttccccggg tcgctggccg tgtcggcgcg cacgggggca ggcgtggccg cgctcctgca
     2821 gcgggtgagc gagctgctgc cgcagtcccg gcgcgtgctg gaggcggtgg tgccctacga
     2881 gcgctccgac ctggtggcga gagcgcaccg ggagggggag gtgctcaaag aggagcaccg
     2941 tccggagggc acgtacctgg tggccaacct ggagcgcgac ctcgccgagg cgctgcgcgc
     3001 ctacgcccag aacgacccgt tcgcctgagc ggcgctcagc gcccggcgtc gccgttggcg
     3061 gggggagcgc ccggcgcctc ctccggagtc accgactcgc gctgacagac cggacagcgg
     3121 ggctggcgct gatcgcccgg ctccgcaggg gcgtccgggt agaaccagcg cctgcagttg
     3181 ggacaccagc gccaccggga tggcattgcc accgactcct tgttccgggc atgcgacaac
     3241 ggggcccgcc tccaagcggt ccagggaggc ggccccacgg ttggacgcac tttcgcgtgc
     3301 cggtcgcacc ccgtcaagaa gatcggggtc gggcgatacg tcgggttcag acgctgcgca
     3361 gcacggcgac gaccttgccc agaatgctcg agtcctggtc ggcggtgacc gggatcggct
     3421 cgtagccgtc gttggccggg tccaggaaga cctcgccggc cttggtgcgg cggaagaact
     3481 tcaccgtcgc ctcgccgtcg accagggccg cgcacatgtc gccctgctcg acggtcggct
     3541 gctggcggat cacgacgtag tcgccgggca ggacgccggc ctgcaccatc gactcgcccc
     3601 ggacccggag catgaacagc gtgccctcgc cgaccagttc ctggggcagg gggaacacct
     3661 gctccacccg ctcctcggcg atgatcggcg cgccggcggc gatctcgccc agcagcggca
     3721 cctgcttgga gcgcaccacc ggcagggaca gctccgtgtc gggatcgaag cgcacctcga
     3781 tcgcgcgcgg ccgggacggg tcccgccgca ggtagccgcc gcgctcgagc gccgcgagct
     3841 ggctgtgcac gctggacggc gagcgcaggc ccacggcctc gccgatctcc cgcacgctgg
     3901 gcgggtagcc ccgcgtcgcc acgctctccc ggatcatctc caggatccgc tgctgccgct
     3961 ccgtgagcgc catctgccgc ctccctgccg cctggtcgtg agcccgccgc gccgtgcggt
     4021 gcgccggcgt gccgtgggga gtgggtgtca cacccctgcg gcacgctacg cctcgaagcc
     4081 gacggaatca aacatctgtt cggattcggt gttgacaacc gaacgcctgt tcgccaggat
     4141 agacgaacag gagttcgagt caaggacgtt ccccacaggg agcaggcggc ggacgaggag
     4201 cgtggtgcag atggcaacgg tgcagcaggt ggggtaccgc cgccgcccga cgcgggcggc
     4261 gtactggcgc cggcgcgccg tcgcgctggt cgtcctggtc ctggcgctgg ccggcgcgct
     4321 gacgctgctg gcgcccaact ccggcgccgc gcccgcgcgg gagccggagc cgctgctgct
     4381 ggtggtgggc ccgggggaca ccgtgctcga cctcgtccgg ccgttcgccc cggaggggtc
     4441 agacccccgc gccttcgccg cggaggtgct ggaggcgagc gacctggatg gccgcgccgt
     4501 ccgtcccggc gcggtgctgc ggctgccccg gtcctgagga tcggggcagc cgccaggccc
     4561 ctaccgtgcc tcccgccgtg ccccggccgg cctcgagggc ggctccggcg gtgcaccggc
     4621 cggccgcttg gggggcgcgt ccttctccag gctggccagt tcgcggcgga agtcctccgg
     4681 cgacgtgaag cccttgtaga cgctggcgaa gcgcatgtag ccgacgtggt ccagcacgcg
     4741 cagggcctcc aggacctcgg ccccgacggc ctcgctgggg accacgcggc ggcccatcgc
     4801 ccgcacgcgc gcctccacct gggcgaccgc gcgggcgacg tcggccggct cgatgtcgag
     4861 gttcttggtg gccttggcca tgccggcctc gatcttgtcg cgccggaacg gctcgatcag
     4921 gccgttgcgc ttgcgcaccg ccagcgacag ctgctccacg cgctcgtagg tcgagaagcg
     4981 ctgcccgcag gacaggcatt cgcgccggcg gcggatcgcc tcgccctcct cgctggggcg
     5041 ggagtcgacc accttgtcgt cgtcgaccgc gcagtacggg caccgcatcg gggttcggct
     5101 cctgtcgtcg caaaaatttt catcaacagt gtggacaacg cgctgcacag gccaggtgcc
     5161 cgcaagcgcg ggaccggggc agcggttgtc caccggttac gcacaccccc accccaacat
     5221 ggtgtggtgg cgccgccacg tcgcccacaa aggccgacat cggagcccat ttccggtgtc
     5281 acaggcgggt gatagcgtcg tgactcgacg gtagcaggaa cgggaaggta tccacaagat
     5341 gtggtgtttt acaagtgcaa gcactgctgc atgtggtgcc cgctgccgca gaaggtcctt
     5401 gactcccggc ccatcggtat agcagtctgc gggactccat cacatccttg gaattacaca
     5461 ggggaagccc atggtcacta ctcgcgacgc gctgcaggcc gtgcccggca agacgggccg
     5521 cgccggcttg aagttcaccc ggcacttcac cacgcccggc gtccacccgt acgacgagct
     5581 ggagtgggag gtgcgcgacg ccgtcatcac cgacttcaag acgggcgcgg cggccttcga
     5641 gcagcgcgac gtggagttcc cgaaatcctg gtcgatgaac gccaccacga tcgtggcgca
     5701 gaagtacttc cgcgggcagc tcggcacccc ggctcgtgag cgcagcgtgc gccagatggt
     5761 cgaccgcgtc gccgacacca tcaccgcctg gggcgtgaag gacggctact tcgccgacga
     5821 ggactccgcg gagatcttca acgccgagct caagcacctg ctggtcacgc agaaggcggc
     5881 gttcaactcc ccggtgtggt tcaactgcgg cgtcgaggag gacccgcagt gctccgcctg
     5941 cttcatcctg tccgtggagg acacgatgag ctccatcctc aactggtacg tcgaggaggg
     6001 cacgatcttc aagggcggct cgggctccgg cgtgaacctg tcccgcatcc gctcctccgc
     6061 cgagaagctc aagggcggcg gcgaggcctc cggcccggtg tcgttcatgc gcggcgcgga
     6121 cgcctccgcc ggcacgatca agtccggcgg caagacccgc cgcgcggcca agatggtcat
     6181 cctcaacgtc gaccaccccg acatcgagga gttcatctgg tgcaagcagc gcgaggaggt
     6241 caaggcgcgc gccctgcgtg agctgggctt cgacatggac ctggacggca aggacgccgc
     6301 gtccatccag taccagaacg ccaacaactc cgtgcgcgtg accgacgagt tcatgcgcgc
     6361 cgtggagaac gacgacgact tcgacctgcg cgccgtcacc aacggcgacg tggtgcgcac
     6421 cgtgcgcgcc cgcgacctgt tccgccagat cgcgcaggcc gcctgggagt gcgccgaccc
     6481 gggcgtgcag tacgacggca cgatcaacga ctggcacacc acgcccaacg ccggtcgcat
     6541 caacggctcc aacccgtgct cggagtacat gcacctggac aacagcgcct gcaacctcgc
     6601 gtcgctgaac ctgcgcaagt tcgagtacga cggcgtgttc gacgtcgacg cgttccgccg
     6661 cgccgtggag gtcgtgttca ccgcgcagga gatcatcgtc ggcaacagct cctaccccac
     6721 ggagcgcatc ggcgcgaacg cccgcgccta ccgccagctc ggcatcggct acgccaacct
     6781 cggtggcctg ctgatgagcc agggcctggc gtacgactcc gacgaggggc gtgcctgggc
     6841 cgccgcgatc accgcgctca tgaccggcca cgcgtaccgg acctcggcgg agatcgccaa
     6901 ggtgcagggg ccgttcgacg gcttcgcgca ggaccgcgag ggcgtgctgc gcgtgatcgc
     6961 caagcacggc caggcctgtg aggacatcga tgcggcgctg gccccggaca acatcctgga
     7021 ggccgcgaag cagtcctggg cggacgcgct gatgctgggc accgagtacg gcatccgcaa
     7081 cgcgcaggcg tcggtcctgg cgcccaccgg caccatcggc ctgatgatgg actgcgacac
     7141 caccggcatc gagcccgacc tgggcctggt gaaggccaag aagctggtcg gcggcggcaa
     7201 catgcgcatc gtcaaccaga cggtgcccgt ggccctgcag aagctcggct accagcagga
     7261 gcagatcgag gcgatcgtcg cctacatcga cgagcacggc acgatcgagg gcgccccggc
     7321 gttccgcgac gagcacctga cggtgttcga ctgcgcgatg ggcacgcgct ccatcagccc
     7381 gatgggccac gtgaagatga tggccgcggt gcagccgttc atctcgggcg cgatctccaa
     7441 gacggtgaac ctgccggaga cggccaccgt ggaggaggtc gagcagctgc acctggacgc
     7501 ctggaagctg ggcgtcaagg cgatcgcgat ctaccgcgac aactgcaagg tggcccagcc
     7561 gctggcgctg gccggatcca agaacgagcc gaagaaggcc gggaccaccg aggccgaggc
     7621 ggcgcaggcg ggcgtgtacc gccgccggct gccgaagtcg cggcccagcc agacgatctc
     7681 gttcgcggtc ggcgacgccg acggctacat cacggcgggg gagtacccgg gcgacgggct
     7741 gggcgagatc ttcgtcaagc tcggcaagca gggctccacg ctgtcgggcg tgatggacgc
     7801 gttcgcgatc agcgtgtcgc tgggcctgca gtacggcgtg ccgctggagg cctacgtcaa
     7861 gaagttcatc aacatgcgct tcgagccggc cggcatgacc gacgacccgg aggtgaagtt
     7921 cgccagctcc atcgtggact acatgttccg gcgtctggcg atcgactacc tgcccgcgga
     7981 gaagcgccac gagctgggca tccacacgct cgaggagcgc aacgcgatgc tcgacgccca
     8041 gtacggcggt ggcggcgcgg ctccggccga cgcggcgccg gtcgtggacg ccgagggcca
     8101 gacggtgctg ccgatcgaga agcccaagcc ggtggacgac gtgcacggcg acgcgccgat
     8161 gtgcttccag tgcggggtca ccatgaaccg cgccggcagc tgccacgtct gcccccagtg
     8221 cggcacgacc agcggttgca gctaggaccc ggggtgtgtc gactgagtca tcctgagaag
     8281 gactgactcg acacacccag gccggtgaag ggcgggacgc ccccgaacgt cccgcccttc
     8341 aacgtctgca cggctgccct gtggaaatcg cgtccggcat cggccgtcgt ttccttacca
     8401 tcgccccgcc cggcactcga cagcggaaag gcgggatggg cgatggtaag gaaacgacgg
     8461 cc
//
LOCUS       DATMKC010000408         3557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_60858, whole genome shotgun sequence.
ACCESSION   DATMKC010000408 DATMKC010000000
VERSION     DATMKC010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60858"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..2852)
                     /locus_tag="VNU01_11875"
     CDS             complement(285..2852)
                     /locus_tag="VNU01_11875"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HWH33358.1"
                     /translation="MSAPGDAASARALFDWLPAGEAGRDPDALLDAFTSWASAQGLTL
                     YPAQEEALIEVVSGSNVIVATPTGSGKSMVAAGAHFAALGRDEVSFYTAPIKALVSEK
                     FFALIEQFGADNVGMMTGDAAVNADAPIICCTAEILANLALRQGADADVGVVVMDEFH
                     FYAEPDRGWAWQVPLLELPQAQFVLMSATLGDVSRFTEDLTKRTGRETTVVSSAERPI
                     PLRYDYVLTPVHETVEDLLKSDQAPVYLVHFTQASAVERAQALMSINMCTREEKDAIA
                     EAIGDFRFAAGFGKTLTRLVRHGIGVHHAGMLPKYRRLVERLAQQGLLKVICGTDTLG
                     VGINVPIRTVLFTGLAKFDGDKVRHLKAREFHQIAGRAGRAGFDTAGLVVVQAPDHVI
                     DNEKAKAKAAAAEAKRPPGGKAKGKKVKMKSPPEGFVNWTKATFDRLVEAEPETLTSS
                     FGVSHAMLLNLIARPGDALTALRRLLTENHEPPETQERHMEQAMAMLDGLVAAGVVER
                     LAEPDAEGRTLRLTVDLQPHFALNQPLSPFAVTALDLLDPDAPTHALDVLSVLEATLE
                     DPRQILSAQEWKAKGEAINAMKADGVEYDERMELLEDITHPRPLAELLEAGFEIYARS
                     HPWVADYRLSPKSVARDLSERAMTFADYVQHYELARSEGLVLRYLADAYKALRQTVPA
                     DAKTEALQDLEAWLGELVRQTDSSLLDEWEALSDPAKLAQAAQRVEQGPPPVTANPRA
                     FRVLVRNALFRRVELAARRRWHELGELDGEHGWPADAWREALQGYFAEHGEIGTGPDA
                     RGPKLFMVEEAPRAWRVRQILDDPAGDHDWAITAEVDLAASDEAGVAVVRVTGVGAA"
     gene            complement(2849..3214)
                     /locus_tag="VNU01_11880"
     CDS             complement(2849..3214)
                     /locus_tag="VNU01_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWH33359.1"
                     /translation="MDSVRVDRWLWAVRLFKTRSAAAEACRGGHVHVNGARVKPAAPV
                     RVGDTVRAWAGDRERIVEVARLLERRVGAPEAAECLVDHSPPPPPREEAAAVPLREPG
                     SGRPSKRERRMLDRMRGRP"
     gene            3318..>3557
                     /locus_tag="VNU01_11885"
     CDS             3318..>3557
                     /locus_tag="VNU01_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWH33360.1"
                     /translation="MSDRAPDPKLGPEVATRRTFAIISHPDAGKTTLTETFLLYGGAI
                     HEAGAVKSRKVERAATSDWMKLEQERGISISSTVLH"
BASE COUNT          451 a         1370 c         1243 g          493 t
ORIGIN      
        1 ccaccggcca gcccgaggat ccgccaccga cgcgggcggg tgatggccgg cccgggatcg
       61 taggcccacg gcgccggcgg cacggcccgg tcggccaacg gcggaaccgc cgcctgcccc
      121 tcaacgtccc ccgtggcctc catgacgggc tgatggtagg gcatcgggtg gcccccaccc
      181 cgctgccgcg acagccgtgg acgcggtgcg caccacattg gtccccaggc ggcgcccgag
      241 cagtgtcaga tgaccagccc gacaggccgg aaacccctgc ggccctaggc ggcgcccacg
      301 cccgtgacgc gcaccacggc gacaccggcc tcgtcggagg ccgccaggtc gacctccgcg
      361 gtgatcgccc agtcgtggtc gcccgccggg tcgtccagga tctggcgcac gcgccaggcg
      421 cgcggcgcct cctcgaccat gaacagcttc gggccgcgcg cgtccgggcc ggtgccgatc
      481 tcgccgtgct cggcgaagta cccctggagc gcctcgcgcc acgcgtcggc gggccagccg
      541 tgctcgccgt ccagctcgcc gagctcgtgc cagcggcgtc gggccgccag ctccacccgg
      601 cggaacagcg cgttgcgcac cagcacccgg aaggcgcgcg ggttcgccgt caccggcggc
      661 ggcccctgct ccacccgctg ggccgcctgc gcgagcttgg ccgggtcgct cagcgcctcc
      721 cactcgtcca gcaggctgga gtcggtctgg cgcaccagct cgcccagcca cgcctccagg
      781 tcctgaagcg cctccgtctt cgcgtccgcg ggcacggtct ggcgcagcgc cttgtacgcg
      841 tccgccaggt agcgcaggac caggccctcg gagcgcgcga gctcgtagtg ctgcacgtag
      901 tcggcgaacg tcatggcgcg ctcggacagg tcgcgggcca cggacttcgg cgacagccgg
      961 tagtcggcca cccacgggtg gctgcgcgcg tagatctcga agcccgcctc cagcagctcg
     1021 gccaggggcc tggggtgggt gatgtcctcc aacagctcca tgcgctcgtc gtactcgacg
     1081 ccgtcggcct tcatggcgtt gatcgcctcg cccttggcct tccactcctg cgccgacagg
     1141 atctggcgcg ggtcctccag cgtggcctcc agcacgctca gcacgtccag ggcgtgcgtc
     1201 ggcgcgtccg ggtccagcag gtccagcgcc gtgaccgcga acggcgacag cggctggttc
     1261 agcgcgaagt ggggctgcag gtccacggtc agccgcagcg tgcggccctc ggcgtcgggc
     1321 tcggccaggc gctcgaccac cccggcggcg accagcccgt ccagcatcgc catcgcctgc
     1381 tccatgtggc gctcctgggt ctccggcggc tcgtggttct ccgtcagcag gcggcgcagc
     1441 gcggtgagcg cgtcgcccgg gcgcgcgatc aggttcagca gcatcgcgtg gctgacgccg
     1501 aagctggacg tcagcgtctc cggctcggcc tcgaccaggc ggtcgaacgt cgccttggtc
     1561 cagttgacga agccctccgg cggggacttc atcttgacct tcttgccctt ggccttgccg
     1621 cccggcggtc gcttcgcctc ggcggccgcc gccttcgcct tggccttctc gttgtcgatc
     1681 acgtggtcgg gcgcctgcac caccaccagg ccggcggtgt cgaagccggc gcgcccggcc
     1741 cggccggcga tctggtggaa ctcacgcgcc ttgaggtggc gcaccttgtc gccgtcgaac
     1801 ttcgccaggc ccgtgaacag caccgtgcgg atcggcacgt tgatcccgac gcccagcgtg
     1861 tccgtgccgc agatgacctt gagcaggccc tgctgcgcca ggcgctccac cagccgccgg
     1921 tacttgggca gcatgccggc gtggtgcacg ccgatgccgt ggcgcaccag gcgggtgagc
     1981 gtcttgccga agccggccgc gaagcggaag tcgccgatcg cctcggcgat ggcgtccttc
     2041 tcctcgcgcg tgcacatgtt gatgctcatc agcgcctgcg cgcgctcgac cgccgaggcc
     2101 tgcgtgaagt gcaccaggta caccggcgcc tggtcgcttt tcagcaggtc ctccaccgtc
     2161 tcgtgcacgg gggtgagcac gtagtcgtag cgcagcggga tgggccgctc ggcggagctg
     2221 accaccgtgg tctcgcggcc ggtgcgcttg gtgaggtcct cggtgaagcg gctgacgtcg
     2281 cccagcgtgg ccgacatgag gacgaactgc gcctgcggca gttccagcag cggcacctgc
     2341 caggcccagc cgcggtcggg ctcggcgtag aagtggaact cgtccatgac caccacgccg
     2401 acgtcggcgt cggcgccctg gcgcagcgcc aggttcgcca ggatctccgc ggtgcagcag
     2461 atgatcggcg cgtcggcgtt gaccgccgcg tcgccggtca tcatcccgac gttgtcggcg
     2521 ccgaactgct cgatgagcgc gaagaacttc tcgctcacca gcgccttgat gggcgccgtg
     2581 tagaagctca cctcgtcgcg gccgagcgcg gcgaagtgcg cgccggccgc caccatgctc
     2641 ttgcccgacc cggtcggggt ggccacgatc acgttggagc cggagaccac ctcgatcagc
     2701 gcctcctcct gcgccgggta cagggtcagg ccctgcgcgg acgcccacga cgtgaacgcg
     2761 tcgagcagcg cgtccgggtc gcggccggcc tcgccggcgg gcagccagtc gaacagcgcc
     2821 cgcgcggacg ccgcgtcacc gggcgcgctc aaggcctgcc tctcatccgg tccagcatcc
     2881 ggcgctcgcg cttgctgggc cggccgctgc ccggctcgcg cagcggcacc gcggccgcct
     2941 cctcgcgcgg cgggggcggc gggctgtggt cgaccaggca ttcggccgcc tccggcgcgc
     3001 cgacgcggcg ctccagcagg cgggcgacct cgacgatgcg ctcgcggtcc ccggcccagg
     3061 cgcgcaccgt gtcgccgacc cgcaccggcg cggcgggctt cacgcgcgcg ccgttgacgt
     3121 gcacgtgccc gccccggcac gcctcggcgg cggcggagcg ggtcttgaac agccggacgg
     3181 cccacagcca ccggtcgacg cgcacggaat ccacgcggcc cattgtgccc cggcccccga
     3241 gcgtgccacc gctatcctgg tgaagccgcg gcagcgccgc gcgtccgccg ccccccacca
     3301 catcgcagga gtcacccgtg tccgaccgcg ccccagaccc caaactgggc ccggaggtcg
     3361 ccacccgccg cacgttcgcg atcatctcgc accccgacgc gggcaagacc acgctcaccg
     3421 agacgttcct gctgtacggc ggggccatcc acgaggccgg ggcggtgaag tcgcgcaagg
     3481 tcgagcgcgc cgcgacgtcg gactggatga agctggagca ggagcgcggc atctcgatca
     3541 gctccacggt gctgcac
//
LOCUS       DATMKC010000409        11182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_121536, whole genome shotgun sequence.
ACCESSION   DATMKC010000409 DATMKC010000000
VERSION     DATMKC010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121536"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VNU01_11890"
     CDS             complement(<1..1339)
                     /locus_tag="VNU01_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33361.1"
                     /translation="MSDFENRPAGQDGDQGRDRLEELLRRSLETRAQDVRPDPGTWQR
                     VSARVERGRRLRLALAGAGGVAAIALAAVVLPSLLDRGTVELVPPATEAPEVEPTDPP
                     DSGGEAPCGGPEHVAAAFARTGEGLEGTLWAHCDDGSERMLSGGRSDGARDINPAFAP
                     DGSALVFERVDRDGLSTLVYLELATGEQQVLGGGALPAFAPDGRLAWVQPVDGGQDLI
                     LIGEPFGEPEMEFPVIDGTDGEWFDARRLAWDATGDTLYAEIGYEESVVYAYEPDSGE
                     GRRVDEPVAEERYGAPSTAPEGLGVAVLARCCLVNDGQAFDRADLRLVDSDDARLLNQ
                     MPEDFDADGDVWTAYAPMGELRQVEGTGVSWTGGTRAAWLVGDGDGLWIVEGDGTVHP
                     MGLKVSSGAANPALGSGGPAPRPDGMAPEQDGLAHDAGVTRQRIWQAAKAADVE"
     gene            complement(1340..1831)
                     /locus_tag="VNU01_11895"
     CDS             complement(1340..1831)
                     /locus_tag="VNU01_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HWH33362.1"
                     /translation="MTERVDFADIFAAHHADALRLAYLLTGDRHRAEDVVSEAFVKVF
                     RRWDAGAVEQPRAYIRRAVVNESNSRFRRLALERREAAKQSGDERGSRAADEQLADQD
                     LVFAALRRLPERQRTALVLRFYQDLSERDIADAMGVSQGTVKSSISRGLDRLRAELGE
                     EVF"
     gene            complement(1949..3493)
                     /gene="metG"
                     /locus_tag="VNU01_11900"
     CDS             complement(1949..3493)
                     /gene="metG"
                     /locus_tag="VNU01_11900"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWH33363.1"
                     /translation="MSGQKDTFYVTTPIYYVNDVPHIGHAYTTVAADVLARWRRLNGD
                     RVHFLTGTDEHGQKVAQAAEQRGMSPQEHVDDIIPRWTELLDLLRISNDDFIRTTQER
                     HKARVQEFMQRLFEQGDLYKNTYAGPYCVRCEAYYTVEELLDGELCPIHKKPVDRMEQ
                     ENWFFRLSKYQDALLKLYADHPEFVRPESRLNEVRTFVESGLQDISASRSAFEWGVDV
                     PWEPGHVFYVWFDALLNYVTAAGYGADDAAFARRWPADVHLIGKDIIRFHAVYWPAML
                     MAGGLDVPKQVYAHGFLLVGGEKMGKSNATGIAPATLVDHFGVDAYRYYFLREISFGQ
                     DGTFSWESMEQRYTTDLANDLGNLANRVLNMVGSYFDGVAPEPTGEEGDAEAALAKDL
                     RTAVEGARAYDALDFKKALEDLWVLVGGVNRYVEARAPWALHKQGARDDLARCLYTCL
                     DMLRIIAVLISPVMPDAAVALWAKLGLAQPLEEQRYPDAARPGLLPAGSRTERGGVLF
                     PKLAED"
     gene            3551..3841
                     /locus_tag="VNU01_11905"
     CDS             3551..3841
                     /locus_tag="VNU01_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWH33364.1"
                     /translation="MDAPGARGIRRKVDDLGRVVIPSSIRRALGIKDGETLEFAVEGD
                     RVILTRAADRCLFCGGEEELTSYRERAVCWSCAAALRAMDRERTGDPPPRPF"
     gene            3964..4371
                     /locus_tag="VNU01_11910"
     CDS             3964..4371
                     /locus_tag="VNU01_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWH33365.1"
                     /translation="MAKYLLLKHYRGAPPAVNDVPFTEWTPEEFDAHVQYMRDFAERL
                     KQTGEFVDTQALTPEGMWVRSDGEGRPPVTDGPFAETKDLIAGWMVIDVDSRERAVEL
                     AAELAAAPGAGGKPIHEWLELRTFLTEPPTITE"
     gene            4372..5517
                     /locus_tag="VNU01_11915"
     CDS             4372..5517
                     /locus_tag="VNU01_11915"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HWH33366.1"
                     /translation="MDDALLRRLTPAVIGVLCRRGADFAAAEDAVQDALLEALRVWPD
                     QPPRDPKGWLVTVAWRRFLDGVRAEASRQRRELLVDEPAAPEPAEAADDTLALYFLCA
                     HPSLSPASAIALTLRAVGGLTTRQIARAYLVPEATMAQRISRAKRAVSGVRLDQPGDV
                     STVLRVLYLVFNEGYSGELDLAAEAIRLARQVAAMTRHPEAAGLLALMLLHHARRAAR
                     TRPDGGLVPLAEQDRSLWDSALVAEGVEILQSALARDRLGEYQAQAAIAALHADARTA
                     GETDWVQIVEWYDELVALTGSPVARLNRAVAVGEADGPRAGLAALAELDPSLPRHTAV
                     AAYLHERDGDLETAARLYAEAARSAANVAERDHLTRQAARLNARWRG"
     gene            complement(5551..6393)
                     /gene="rsmI"
                     /locus_tag="VNU01_11920"
     CDS             complement(5551..6393)
                     /gene="rsmI"
                     /locus_tag="VNU01_11920"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWH33367.1"
                     /translation="MGQRSGDGRLVVVATPIGNLEDLSPRAARALSEADVVAAEDTRR
                     TRVLLDHIGVRVPMVSHHEHNEDQRIPELLARVEAGDVVAVVSDAGVPGLADPGFRLV
                     RAAVERGLAVEAVPGPAAAIQALVLSGLPMERFVFEGFLPRKGVARRERLAALAAEPR
                     TMVLYVSPHRAADDLDDLAGALGAERHGALGRELTKLHEEVWRGTLAELADRARDGVR
                     GEATLVVAGAPATGPAELTDEQLAALVHERMAAGLSRKDAAAEVARATGVPKRSAYDA
                     SLGR"
     gene            6423..7856
                     /locus_tag="VNU01_11925"
     CDS             6423..7856
                     /locus_tag="VNU01_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HWH33368.1"
                     /translation="MSAPAMAPATEHSTATRVGAAAILLPLLLVAVAGTLRFYRLGHP
                     PRIYFDEVYYANDAKEVLDHGVERGFAVHPPVGKWVIAGGIALFGDDSFGWRFGVAVA
                     GTLTVLATYLLGLRLFRRRGVAALAAFLLAIDGLAFTMSRIAMLDMILTLFVVTGAWL
                     LLVDRDRMWADLPEGPVEPGEPPPHRPRTYRWLAGVAFGLALATKWSGLLAIGAAGLF
                     LIGNELLWRRRTTGRPLHRWWRAAVSIGASLVLVPLLVYAASYGGWFANFEQTRQGQE
                     QCRDGCALALPDIAGTWADEQLQIYRFHRDLTAEHPYRASPATWPLMLRPVAYYYESC
                     DGEKLANDECVVEPGNVAEVLGMGNPAIWWLALGAYAVVGWYAAARRDWRAGLLLVFL
                     LGQFLPWLATSRPAFLFYTVPMLPFMTLALAYAAWRSREEPALRWVPFAILALAVAGF
                     LFWYPVLAGVEMPKEAWQLRIWTSRWV"
     gene            complement(7863..8219)
                     /locus_tag="VNU01_11930"
     CDS             complement(7863..8219)
                     /locus_tag="VNU01_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33369.1"
                     /translation="MREKIERHIDEHEHGEVPPPAHTPTSGREMEQELDKGLWGPGAL
                     GTRSQLIGGAGGLVVGGIIGGVLGLLAGLLLFAGEMTSVVITIVVGVVAGSTALGIAG
                     GFVWNRGKQERGDPDV"
     gene            8437..8814
                     /locus_tag="VNU01_11935"
     CDS             8437..8814
                     /locus_tag="VNU01_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH33370.1"
                     /translation="MTPRGPDRPSKPTRPPRAFVPEWPHGPVSRVPEAEGGEATEWLA
                     EQAARLAGQIMRRRLERGLRRSQLAARTGLRPNTILDVEDGRSWPDFRTIGLIAWALE
                     ADLEFEPRVALRAAEPVSRYRTD"
     gene            8959..9339
                     /locus_tag="VNU01_11940"
     CDS             8959..9339
                     /locus_tag="VNU01_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH33371.1"
                     /translation="MTAQPTSPKPPPAKPRRPPREYLRGTWPDSPVRRVPEEEGGEAV
                     EHLVEQAARLARQISSRRLSRGLSRSRVAALTGLRPNTVLDVEEGRSWPDLRTLGLLA
                     WALEADLELEPRVPLRARDRWSRT"
     gene            9404..9922
                     /locus_tag="VNU01_11945"
     CDS             9404..9922
                     /locus_tag="VNU01_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33372.1"
                     /translation="MRTWKAAERALRRETGLTVGRLRDSHPIPLVIAAGGARPRAVVA
                     LGGVDPHDPMDTVGDLACFAAALAPSRVIVALSVTRSPRARRGMLLCCDVRAVADGLV
                     EVTRAWPWYRLGRHVWWPRVEVRQWESPMDLLPGIAAAILKRRDRDHSDELVERMLAY
                     TLATGHRVRLAR"
     gene            9949..10191
                     /locus_tag="VNU01_11950"
     CDS             9949..10191
                     /locus_tag="VNU01_11950"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWH33373.1"
                     /translation="MCRSIQQLRGAEPPATTQEVREAALQYVRKISGYRVPAKANEAA
                     FAAAVDEVAAATQRLLDGLVVAPGSRPPELVQRRPR"
     gene            10188..>11182
                     /locus_tag="VNU01_11955"
     CDS             10188..>11182
                     /locus_tag="VNU01_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HWH33374.1"
                     /translation="MTAAATPAPHARTGPAQARRPHVAVWLVWAAACLAVLQAATTPP
                     AALLLIGVAALTVEACGDRRRLAGAFPALVGAAAFFALLRVLLTALTTHAGEDVLARL
                     PSFELPALLGGFTVGGTLHRAVLLRSLSEGLVVVAVIATLAAFNAVVSHHELLRLLPR
                     AFHELALVVSVAVAFVPSALAAVGAVREADHARAGAPVGSRRGWARRLGAVLETSLER
                     AILLSESMESRGFGHTGSGRAERRGSRLALLGLLSCSGAFGALLARQPAVALAAGALG
                     AAALLAAVLLVSASRPRAGLPARRLERADLAMIAVLLGAPAGLLALPDTAAALRW"
BASE COUNT         1334 a         4212 c         4138 g         1498 t
ORIGIN      
        1 cctccacgtc ggcggccttg gcggcctgcc agatgcgctg gcgggtgacg ccggcgtcgt
       61 gggcgaggcc gtcctgctcg ggcgccatgc cgtccgggcg cggcgccggc ccgccggagc
      121 ccagcgccgg gttcgccgcc ccggagctga ccttcagccc catcgggtgc acggtgccgt
      181 caccctcgac gatccacagg ccgtcgccgt cgccgaccag ccacgcggca cgggtgccgc
      241 ccgtccagct cacgccggtg ccctcgacct ggcgcagctc gcccatgggc gcgtacgccg
      301 tccacacgtc gccgtcggcg tcgaagtcct ccggcatctg gttgagcagc cgggcgtcgt
      361 cgctgtcgac caggcgcagg tcggcgcggt cgaacgcctg cccgtcgttc accaggcagc
      421 agcgcgcgag cacggccacg cccagcccct ccggggcggt ggacggcgcg ccgtagcgct
      481 cctcggcgac cggctcgtcc acgcgccggc cctcgccgct gtccggctcg taggcgtaga
      541 ccacgctctc ctcgtagccg atctcggcgt acagcgtgtc gccggtggcg tcccaggcca
      601 ggcggcgggc gtcgaaccac tcgccgtcgg tgccgtcgat caccgggaac tccatctccg
      661 gctcgccgaa cggctcgccg atgaggatga ggtcctggcc gccgtcgacg ggctgcaccc
      721 acgccaggcg gccgtcgggg gcgaacgccg gcagcgcgcc gccgccgagc acctgctgct
      781 cgccggtggc gagctccagg tagaccagcg tcgacaggcc atcccggtcc acgcgctcga
      841 acaccagggc gctgccatcg ggggcgaacg ccgggttgat gtcgcgcgcg ccgtcggagc
      901 ggccaccgga cagcatgcgc tcggagccgt cgtcgcagtg ggcccacagg gtgccctcca
      961 ggccctcgcc ggtgcgggcg aaggcggcgg ccacgtgctc cggaccgccg cagggcgcct
     1021 cgccgccgga gtccggggga tccgtcggct cgacctccgg ggcctcggtc gcgggcggca
     1081 ccagctccac ggtgccccgg tccagcaggg acgggagcac cacggcggcc agcgcgatcg
     1141 cggccacgcc gcccgcgccg gccagcgcaa ggcgcaggcg ccgcccacgc tccacgcggg
     1201 cggagacccg ctgccaggtc cccgggtcgg gccgcacgtc ctgcgcgcgc gtctccaggg
     1261 agcggcgcag cagctcctcc aggcggtcgc gcccctggtc gccgtcctgg ccggcgggac
     1321 ggttctcgaa gtcgctcatc tagaacacct cctcgccgag ctcggcgcgc aggcggtcca
     1381 gcccgcggga aatgctgctc ttgaccgtgc cctggctgac gcccatcgcg tcggcgatgt
     1441 cgcgctcgga caggtcctgg tagaagcgca ggaccagggc ggtgcgctgg cgctccggca
     1501 gccggcgcag cgcggcgaag accaggtcct ggtcggcgag ctgctcgtcg gcggcgcgcg
     1561 agccgcgctc gtcgccggac tgcttggcgg cctcgcggcg ctccagcgcc aggcggcgga
     1621 agcgcgagtt ggactcgttc accaccgcgc ggcggatgta ggcgcgcggc tgctccacgg
     1681 ccccggcgtc ccagcggcgg aacaccttca cgaacgcctc gctcacgacg tcctcggcgc
     1741 ggtggcggtc accggtcagc aggtacgcca gtcgcagggc gtcggcgtgg tgggccgcga
     1801 agatgtccgc gaagtccacg cgctcggtca tggaacggct caacgtggtg gtcctcagtt
     1861 cggcacgggg gacgctcagc acaccgctca cctcctttcg tcacccgcac tgacgcgcga
     1921 gtggcgtggg aggttgagcc gggcccggtc agtcctccgc gagcttgggg aacagcaccc
     1981 cgccgcgctc cgtgcgggaa ccggcgggca gcagcccggg ccgtgccgcg tcggggtagc
     2041 gctgctcctc cagcggctgg gcgaggccga gcttggccca gagtgccacg gccgcgtccg
     2101 gcatcaccgg cgagatgagc accgcgatga tccgcagcat gtccaggcac gtgtacaggc
     2161 agcgggccag gtcgtcgcgg gcgccctgct tgtgcagcgc ccagggggcg cgcgcctcca
     2221 cgtagcggtt cacgccgccg accaggaccc acaggtcctc cagcgccttc ttgaagtcca
     2281 gcgcgtcgta cgcccgcgcg ccctcgaccg ccgtgcgcag gtccttggcg agcgccgcct
     2341 ccgcgtcgcc ctcttcgccg gtgggctcgg gcgccacgcc gtcgaagtag ctgcccacca
     2401 tgttgagcac gcggttggcc aggttgccca ggtcgttggc gaggtcggtg gtgtagcgct
     2461 gctccatgga ctcccaggag aacgtgccgt cctggccgaa ggagatctcg cgcaggaagt
     2521 agtagcggta ggcgtccacg ccgaagtggt cgaccagcgt cgcgggcgcg atgcccgtgg
     2581 cgttggactt gcccatcttc tccccgccca ccagcaggaa gccgtgcgcg tacacctgct
     2641 tgggcacgtc cagcccgccg gccatgagca tcgcgggcca gtacacggcg tggaagcgga
     2701 tgatgtcctt gccgatgaga tggacgtcgg ccggccaccg ccgggcgaac gcggcgtcgt
     2761 cggcgccgta gccggccgcg gtcacgtagt tgagcagcgc gtcgaaccac acgtagaaga
     2821 cgtggccggg ctcccacggc acgtccacgc cccactcgaa cgcgctgcgg gaggcggaga
     2881 tgtcctgaag gccgctctcg acgaacgtcc tgacctcgtt gaggcggctc tccggccgca
     2941 cgaactcggg gtggtcggcg tacagcttca gcagcgcgtc ctggtacttg gacaggcgga
     3001 agaaccagtt ctcctgctcc atgcggtcca cgggcttctt gtggatcggg cagagctcgc
     3061 cgtcgagcag ttcctccacc gtgtagtacg cctcgcagcg cacgcagtac gggcccgcgt
     3121 aggtgttctt gtacaggtcg ccctgctcga agagccgctg catgaactcc tgcacgcgcg
     3181 ccttgtggcg ctcctgcgtg gtgcggatga agtcgtcgtt ggagatgcgc agcaggtcca
     3241 agagctccgt ccagcgcggg atgatgtcgt cgacgtgctc ctgcggcgac atgccccgct
     3301 gctcggcggc ctgcgcgacc ttctggccgt gctcgtcggt gccggtgagg aagtgcaccc
     3361 ggtcgccgtt gagccgccgc cagcgcgcca gcacgtccgc ggccaccgtg gtgtaggcgt
     3421 gcccgatgtg cggcacgtcg ttcacgtagt agatcggcgt ggtgacgtag aacgtgtcct
     3481 tctggcctga catcgtcaac ggcctaccgc ttcgctgctt gagtccatgc ggccgatact
     3541 atccgggccc atggacgctc ccggggccag aggcatccgc cgcaaggtcg acgatctcgg
     3601 ccgcgtggtg atcccctcca gcatccgccg ggccctgggc atcaaggacg gcgagacgct
     3661 ggagttcgcc gtcgagggcg accgcgtcat cctcacccgc gccgccgaca ggtgcctgtt
     3721 ctgcggcggc gaggaggagc tgaccagcta ccgcgagcgc gccgtgtgct ggtcgtgcgc
     3781 cgccgccctg cgcgccatgg accgcgagcg caccggcgac ccgccgccgc ggccgttctg
     3841 acaccggccg tcctcgcgcg gatcgccgga aaaagtcccg cgacgatgtc gagaatcggc
     3901 gagcggctcc gtccccgggg tgaacgcggc caccatgggc cgcccgcatc gaggaggaac
     3961 gcgatggcga agtacctgct gctcaagcac taccgggggg cgccgccggc ggtcaacgac
     4021 gtgccgttca ccgagtggac gccggaggag ttcgacgcgc acgtgcagta catgcgcgac
     4081 ttcgccgagc ggctgaagca gaccggcgag ttcgtggaca cgcaggcgct gacgccggag
     4141 ggcatgtggg tccgctccga cggcgagggg cggccgccgg tcaccgacgg gccgttcgcc
     4201 gagaccaagg acctgatcgc cggctggatg gtgatcgacg tcgacagccg ggagcgcgcc
     4261 gtggaactgg ccgccgaact ggccgccgct ccgggtgccg gcgggaagcc gatccacgag
     4321 tggctggaac tgcgcacgtt cctgaccgag ccgccgacca tcaccgaata ggtggacgac
     4381 gccctgctgc gccggctcac gcccgccgtg atcggtgtcc tctgccgccg cggagcggac
     4441 ttcgccgcgg cggaggacgc cgtgcaggac gccctgctgg aggccctgcg ggtctggccg
     4501 gaccagccgc ccagggaccc caagggctgg ctggtcaccg tggcgtggcg caggttcctg
     4561 gacggcgtgc gcgccgaggc gtcgcggcag cggcgcgagc tgctcgtcga cgagccggcc
     4621 gcgccggaac cggcggaggc ggccgacgac acgctggcgc tgtacttcct gtgcgcgcac
     4681 ccgtccctgt cgcccgcctc cgcgatcgcc ctcaccctgc gcgccgtcgg tggcctgacc
     4741 acgcggcaga tcgcgcgggc ctacctggtg ccggaggcca ccatggcgca gcgcatcagc
     4801 cgcgccaagc gtgccgtgtc cggcgtgcgc ctggaccagc ccggcgacgt gtccaccgtg
     4861 ctgcgcgtgc tctacctggt cttcaacgag ggctactccg gggagctgga cctggccgcc
     4921 gaggcgatcc gcctggcccg ccaggtcgcc gccatgaccc ggcatcccga ggcggccggc
     4981 ctgctggcgc tcatgctgct gcaccatgcc cggcgcgcgg cccgcacccg cccggacggc
     5041 ggcctggtac cgctggccga gcaggaccgc tcgctgtggg acagcgcgct ggtcgccgag
     5101 ggcgtcgaga tcctgcagag cgccctggcg cgcgaccggc tgggcgagta ccaggcgcag
     5161 gccgccatcg cggcgctgca cgccgacgcg cggacggcgg gcgagaccga ctgggtgcag
     5221 atcgtcgagt ggtacgacga gctggtggcg ctcaccggca gcccggtcgc ccgcctgaac
     5281 cgggccgtcg ccgtgggcga ggcggacggc ccgcgggcgg gccttgccgc cctggccgag
     5341 ctcgacccgt cgctgccgcg ccacaccgcc gtcgcggcgt acctgcacga gcgcgacggg
     5401 gacctggaga ccgcggcgcg gctgtacgcc gaagccgccc ggtcggcggc gaacgtcgcg
     5461 gagcgcgacc atctcacccg tcaggccgcg cggctcaacg cgcgatggcg cggctgagcg
     5521 tctcgcgatc cgccgcctct cccccggggt tcagcgcccc aggctggcgt cgtaggccga
     5581 gcgcttgggc acgccggtcg cccgcgcgac ctccgcggcc gcgtccttgc gcgacagccc
     5641 cgcggccatg cgctcgtgga ccagcgcggc aagctgctcg tcggtgagct ccgcggggcc
     5701 ggtcgccggc gcccccgcca ccaccagcgt ggcctcgccg cgcacaccgt cccgggcgcg
     5761 gtccgcgagc tcggcgagcg tgccgcgcca cacctcctcg tgcagcttgg tgagctcccg
     5821 gcccagcgcg ccgtgccgct cggcgcccag cgcgccggcc aggtcgtcca ggtcgtccgc
     5881 cgcgcggtgc ggcgacacgt acagcaccat cgtgcgcggc tcggcggcca gcgccgccag
     5941 ccgctcgcgg cgcgcaacgc ccttgcgcgg caggaagccc tcgaacacga agcgctccat
     6001 gggcagcccg gacagcacca gcgcctggat cgccgcggcg ggtcccggca cggcctccac
     6061 ggcgaggccc cgctccaccg ccgcgcgcac caggcggaag cccgggtcgg ccaggcccgg
     6121 cacgccggcg tcggacacca cggccaccac gtcgccggcc tccacgcgcg cgagcagctc
     6181 ggggatgcgc tggtcctcgt tgtgctcgtg atggctgacc atcggcacgc gcaccccgat
     6241 gtggtccagc agcacgcgcg tgcgccgcgt gtcctcggcg gcgaccacgt cggcctcgct
     6301 cagcgcgcga gcggcccggg gcgacaggtc ctccaggttg ccgatcggcg tggcgacgac
     6361 cacgagccgc ccgtcgccgc tgcgctggcc catcgggtgc tcctccgtag actgtcgccc
     6421 tcatgtccgc ccccgccatg gctccagcca ccgagcactc gaccgcgacg cgcgtgggcg
     6481 cggccgcgat cctgctgccc ctgctgctgg tggcggtggc ggggacgctg cggttctacc
     6541 ggctgggcca cccgccgcgc atctacttcg acgaggtcta ctacgccaac gacgccaagg
     6601 aggtcctgga ccacggcgtc gagaggggct tcgcggtcca tcccccggtg ggcaagtggg
     6661 tgatcgccgg cggcatcgcg ctgttcggcg atgacagctt cggctggcgc ttcggcgtcg
     6721 cggtcgccgg caccctgacg gtcctggcga cctacctcct gggcctgcgg ctgttccgcc
     6781 gccgcggcgt cgcggcgctc gcggccttcc tgctggccat cgacggcctg gcgttcacga
     6841 tgagccgcat cgccatgctg gacatgatcc tgacgctgtt cgtggtcacc ggcgcatggc
     6901 tgctgctggt ggaccgggac cgcatgtggg ccgacctgcc ggaggggccg gtggagcccg
     6961 gcgagccgcc gccgcaccgg ccgcgcacgt accgctggct ggccggcgtg gcgttcggcc
     7021 tggcgctggc gaccaagtgg tcgggcctgc tggccatcgg cgcggccggg ctgttcttga
     7081 tcggcaacga gctgctgtgg cggcggcgca ccaccggacg cccgctgcac cgctggtggc
     7141 gcgccgccgt gagcatcggc gccagcctcg tgctggtccc gctgctggtc tacgcggcct
     7201 cctacggcgg ctggttcgcc aacttcgagc agacccgcca gggccaggag cagtgccgcg
     7261 acggctgcgc gctggccctg cccgacatcg cggggacctg ggccgacgag cagctgcaga
     7321 tctaccgctt ccaccgcgac ctcaccgccg agcaccccta ccgcgcctcg ccggccacgt
     7381 ggccgctgat gctgcgcccg gtggcctact actacgagtc ctgcgacggc gagaagctcg
     7441 ccaacgacga gtgcgtggtg gagcccggca acgtcgccga ggtgctgggc atgggcaacc
     7501 cggcgatctg gtggctggcg ctgggcgcgt acgcggtggt gggctggtac gccgccgcgc
     7561 ggcgggactg gcgcgcgggc ctgctcctgg tgttcctgct ggggcagttc ctgccgtggc
     7621 tggcgacgtc ccggcccgcg ttcctgttct acaccgtgcc gatgctgccg ttcatgacgc
     7681 tcgcgctggc ctacgccgcg tggcgctccc gcgaggagcc ggcgctgcgc tgggtgccgt
     7741 tcgccatcct ggcgctggcc gtggccggct tcctgttctg gtacccggtg ctggcgggcg
     7801 tggagatgcc caaggaggcc tggcagctcc gcatctggac gtcgcgctgg gtctgatcgg
     7861 cgtcagacgt ccgggtcgcc gcgctcctgc ttgccgcggt tccagacgaa gccgccggcg
     7921 atgcccaggg cggtcgagcc ggccaccacg cccaccacga tcgtgatgac gacgctggtc
     7981 atctcgccgg cgaacagcag caggccggcc agcagcccca ggacgccgcc gatgatgccg
     8041 ccgacgacca ggccgccggc gccgccgatg agctggctgc gcgtgccgag cgcgccgggg
     8101 ccccacagtc ccttgtcgag ctcctgctcc atttcacgtc ccgacgtggg ggtgtgcgcg
     8161 ggaggcggca cctcgccgtg ctcgtgctcg tcgatgtggc gttcgatctt ctcgcgcatc
     8221 tggcctcgct cctcgtggac gctcctcgcg ggtgtccttc ccgccgggca gcgcccccat
     8281 gcctaggcgc gcaggccgtc caggacgcca cggcgcgacg ctcgcggcac gctcggccgg
     8341 tccaccgata gcaagcgggg cggtccagcg ataggacggt caccgggccg tcccagacgg
     8401 cggttcgggg ccgtcacgtc cctcagcatg gccgccatga cgccgcgggg gcccgatcga
     8461 ccatccaagc cgacccggcc gccgcgcgcc ttcgtgcccg agtggccgca cggcccggtg
     8521 tcgcgcgtgc cggaggcgga aggcggcgag gcgaccgagt ggctggcgga gcaggccgcg
     8581 cgcctggccg gccagatcat gcggcggcgc ctggagcggg ggctgcgccg cagccagctc
     8641 gccgcccgca ccggcctgcg ccccaacacg atcctggacg tcgaggacgg ccggagctgg
     8701 ccggacttcc gcaccatcgg gctgatcgcc tgggcgctgg aggcggacct ggagttcgag
     8761 ccgcgcgtgg cgctgcgcgc ggcggagccg gtgtcgcgct accgcacgga ctgaggcgcg
     8821 cccgcgtcaa gaccggcgaa aaccactggc gcactcactc gaacatatgt acgatctgtt
     8881 tcaccgtacc gaccactgac gagggacggc ggcgccggca gagcgccgct cgtcaggcga
     8941 cgagcaggcc acctcgccat gaccgcccag ccgaccagtc ccaagccgcc gccggccaag
     9001 ccgcgccggc ccccgcgcga gtacctgcgc gggacctggc ccgacagccc ggtccgccgc
     9061 gtccccgagg aggagggcgg cgaagccgtg gagcacctgg tggaacaggc cgcccggctg
     9121 gcgcgccaga tctcctcgcg gcgcctgagc cgcggcctgt cgcgcagccg cgtcgccgcg
     9181 ctgaccggcc tgcgccccaa caccgtgctg gacgtggagg agggccggag ctggccggac
     9241 ctgcgcaccc tgggactgct ggcgtgggcg ctcgaagcgg acctggagct ggagccgcgc
     9301 gtgcccctgc gggcgcgcga ccgctggagc cgcacctgac ttgtccacag gaaacggggc
     9361 ggggggctgg cccttcgcgc tcgtcgcggc caggatgccg cccatgcgga cctggaaggc
     9421 agccgagcga gcgctgcggc gagagacggg actgaccgtc ggccgcctgc gcgacagcca
     9481 ccccatcccc ctcgtcatcg ccgccggcgg cgcccggccg cgcgccgtgg tcgcgctcgg
     9541 cggcgtcgac ccccacgatc ccatggacac cgtcggcgac ctggcgtgct tcgccgccgc
     9601 gctggcgccg agccgggtga tcgtcgcgct gtcggtcacc aggtcacccc gcgcgcgcag
     9661 ggggatgctg ctgtgctgcg acgtgcgcgc cgtcgccgac gggctggtgg aggtgacgcg
     9721 cgcgtggccg tggtaccggc tgggccggca cgtgtggtgg ccgcgcgtgg aggtgcggca
     9781 gtgggagagc cccatggacc tgctgccggg catcgccgcg gcgatcctca agcggcgcga
     9841 ccgggaccac tcggacgagc tggtcgagag gatgctggcc tacacgctgg ccaccgggca
     9901 ccgggtgcgc ctggcccgct gacgaggccg ccgcgtacag tgccccgcat gtgccgaagc
     9961 atccagcagc tgcgcggcgc ggagccgccc gccaccacgc aggaggtccg cgaggcggcc
    10021 ctgcagtacg tgcgcaagat cagcggctac cgcgtgcccg ccaaggccaa cgaggcggcg
    10081 ttcgcagccg cggtcgacga ggtcgccgcg gcgacccagc ggctcctcga cggcttggtc
    10141 gtcgcccccg ggtcgcggcc gcccgagctc gtccaacgcc gcccacggtg accgccgccg
    10201 ccacgccggc accgcacgcg cgcaccgggc cggcgcaggc gcgccgcccg cacgtcgccg
    10261 tgtggctggt gtgggccgcc gcgtgcctgg ccgtgctgca ggcggccacc acgccgccgg
    10321 cggcgctgct gctcatcggc gtggcggcgc tgaccgtgga ggcgtgcggc gaccggcggc
    10381 gcctggccgg cgcgtttccc gccctggtcg gcgcggcggc gttcttcgcg ctgctgcgcg
    10441 tgctgctcac cgcgctgacc acccacgccg gcgaggacgt gctggcgcgc ctgccgtcct
    10501 tcgagctgcc cgcgctgctc ggcggcttca cggtgggcgg cacgctgcac cgcgcggtgc
    10561 tgctgcgctc gctgagcgag ggcctggtgg tggtggcggt catcgcgacc ctggcggcgt
    10621 tcaacgccgt ggtgagccac cacgagctgc tgcgcctgct gccgcgcgcg ttccacgagc
    10681 ttgcgctggt ggtcagcgtg gcggtcgcgt tcgtgccctc ggcgctggcc gccgtcggcg
    10741 cggtgcgtga ggccgaccac gcgcgcgcgg gcgcaccggt cggctcgcgg cgcggctggg
    10801 cgcggcgcct gggcgccgtg ctcgagacgt cgctggagcg cgcgatcctg ctgtcggagt
    10861 ccatggagtc gcggggcttc ggccacacgg gcagcggccg ggcggaacgg cgcggcagcc
    10921 gcctggcgct gctgggcctg ctgtcgtgca gcggcgcgtt cggcgcgctg ctggcgcgac
    10981 agcccgccgt ggcgctcgcg gcgggcgcgc tcggagccgc cgcgctgctc gccgcggtgc
    11041 tgctggtgtc ggcgtcccgg ccgcgcgcgg ggcttccggc ccgccggctg gagcgcgccg
    11101 acctggcgat gatcgcggtg ctgctcggcg cgcccgccgg cctgctggcg ctgccggaca
    11161 ccgccgcggc cctgcgctgg ga
//
LOCUS       DATMKC010000410        11603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_192985, whole genome shotgun sequence.
ACCESSION   DATMKC010000410 DATMKC010000000
VERSION     DATMKC010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192985"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /gene="murJ"
                     /locus_tag="VNU01_11960"
     CDS             <1..1699
                     /gene="murJ"
                     /locus_tag="VNU01_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWH33375.1"
                     /translation="SGPADPVTGPGSAPAGHAPDGAEQPALARNTAVMAAGTLLSRIT
                     GFGRLAAMTAAFGIVEGPLADAYNKANVAPNIVYELVLGGVLSSVLIPVLVETLRRDE
                     AEHRHVTNTITTIAGVVLLAITTAGIAGAPWLAVLFTLGADGPAAEVAELRRVVTLFL
                     RLFLPQVLFYGLTTLWTAYLNAHRHFAAPMFAPVLNNVVVIGVLLWYGAIVDFRAVAE
                     LGRLSAAQLWLLGLGTTAGILAMTLPLWPVSRRHGWAFRPSLDWRHPMVRRVGKLGGW
                     AVLYVALNQVGYLVVIVLTSAIPGDGAFAAYAGAFVFFQLPHGIYAVSVMTALVPGLA
                     EAASAGETARYRAQLAAGIRATALFIVPAAVGLAVLSAPIVRLLLERGAFTAESTELV
                     SAVLTAFAIGLPSFSLFQLFLRAHYALQDTRTPVLVNVAAVSLNIAVDVALFLLLPGQ
                     WKVVGLALGHAAAYTLGAALFALRLRRRIGGLGGRQTAGALLRIAAASAGMGALVSAV
                     ALALRETVGVAGFGAQALQVAAATGIGAVAYLALARLAGVRELDLLKSLVRGRRHAD"
     gene            1734..2138
                     /locus_tag="VNU01_11965"
     CDS             1734..2138
                     /locus_tag="VNU01_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33376.1"
                     /translation="MHRHGEHGGVIVSGLLRTAVWVGLFCVLAFDTGGIVTNRVLLDE
                     ATRVAARQAADSLDRSRGVATASAVRAAREAAEDSVADQPGIEVLDVAVERGEVSVTA
                     RRRARVLVADRIPPLRDHVEAESRASASLGGP"
     gene            2245..3897
                     /locus_tag="VNU01_11970"
     CDS             2245..3897
                     /locus_tag="VNU01_11970"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWH33377.1"
                     /translation="MSGTPPDAPGQPTQPDREAPPRVLGGRYLLEEPIATGGMAMVWR
                     AHDELLARTVAIKILHDHLAANEAFRERFRREAIAAAKLTHPNVVGLFDTGSDDSRVY
                     LVMEYVDGPTLRDVLRDLGSLEPGEAAAIGEKIARALAYAHERGLVHRDVKPANVLIG
                     EDGTVKIADFGIAKAEAGADDLTTPGMVLGTAAYVAPEQVRGEEVDGQADQYALGCLL
                     YEMLTGQQPFKGDTAVATAALRLEHDPLPPRSLRAGIPRGLDDVVLRAMARDPAARHA
                     SLAHLADALAPFAAMDATAALTPLADSGRAAAWAAGPVADDAPTLSGTPPYGYGQPLP
                     PSALPYAGSTAEDSFLRSEGRWIAPILGLVALAGLLVGVGLATGVLEPSSGFPIRIAR
                     ESAPGQAGELIAPVGIDAFDPPPGDGSENDSDLPKILDGDDATSWRTDLYRNSPQFGN
                     LKEGVGFWVDLGASRTLHSVALKTTTPGISYQVRVADGPAPTVEGWRLVGTVESAEGL
                     DQVGFAEPVTARYVLVWITGNLQPQRGRFAAGFAAMAVRGVA"
     gene            3911..4519
                     /locus_tag="VNU01_11975"
     CDS             3911..4519
                     /locus_tag="VNU01_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH33378.1"
                     /translation="MSAADVADEVLVGEWARGDQAAFDELVRRYRRRVYGICYRYFGD
                     AADAEDAAQDAFVALLRKAGTFQGTSSFSTWMYRVTVNACNDLARKRGRRPQRTDTDV
                     STLADDLAGTEDLVGRRELGMVLEGALRELDPDTRAAIVLHDVQGVPYQDIAERLGLA
                     VGTVKSRIHRGHARLAALLTEAGGTLPDGSASNPAKPTSGHA"
     gene            4516..5547
                     /locus_tag="VNU01_11980"
     CDS             4516..5547
                     /locus_tag="VNU01_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33379.1"
                     /translation="MNDYRPPDAQGPAQPEPDEALSAYLLGDLEDGAAAAFEAWLAAD
                     AGLRARLDALAGALAALGGVDDVDEPDGFAERLGARLAAERDVADLSARRERRRRWSA
                     VLTAAAGVVVLAVAGLQVTTGGPPAPRDERVAREMAAGDDAAVEQAAGEALLESDGME
                     LESGAGSADAYRAPEPMADGDGGAAGVEETAADGPADSAGGGSGGAPSMAAADDGQGP
                     AIVDEGASLDDDDQLRARYEGAPESERFRGLPREDAAEVAARHADQVRAADAFASGAR
                     PDRCLDEVGAERDGRIVTVVERLRWQGGEALAYVLALPSETSATVDRFEAWVMGPQGC
                     DILGRVSWR"
     gene            5588..6541
                     /gene="trxB"
                     /locus_tag="VNU01_11985"
     CDS             5588..6541
                     /gene="trxB"
                     /locus_tag="VNU01_11985"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWH33380.1"
                     /translation="MADAADLPAEPVREVLIIGSGPAGLTAAIYAARANLEPLMIEGR
                     EPGGQLMLTTEVENFPGFPTGRMGPELMADMRAQAERFGTEILTADVDEVDLASGSSF
                     TATVGDTVYRARTVIISTGATARWLGLPNEQRLIGRGVSSCATCDGFFFRDRELVVVG
                     GGDSAMEEATFLTKFASKVTVVHRRDELRASKIMQERALKNDKIEFLWNAEVVEVLGA
                     DVVEGVVLRDTLSGEQRDFRTDGLFLAIGHDPTTALFRGQLDLDDDGYILVSEPRTET
                     SVPGVFAAGDVVDRIYRQAITAAGMGCKAAIDAERWLEAQA"
     gene            6959..7285
                     /gene="trxA"
                     /locus_tag="VNU01_11990"
     CDS             6959..7285
                     /gene="trxA"
                     /locus_tag="VNU01_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWH33381.1"
                     /translation="MGTTKSVTDAEWESEVLKSDLPVLVDFWAEWCGPCRMVSPVVEE
                     IAGEKAGALKVLKLNVDDNPETARKYKVMSIPTLMVFQGGDEKKRLVGARGKSQLLQE
                     VDEFLS"
     gene            7348..8442
                     /locus_tag="VNU01_11995"
     CDS             7348..8442
                     /locus_tag="VNU01_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HWH33382.1"
                     /translation="MSDPQRTPPIRLGDEGAPVSDLQRRLVRLGEQDLEVDGRFGPVT
                     LEAVRRFQREHGLAADGVVGPETWRRLVEAGWALGDRLLYQRRVMLRGEDVLELQQRL
                     SRLGFDAGPEDGIFGPLLHAAVEEFQRNAGLEVDGVAGPATVAALRRLHRDHQRGGHV
                     ARLREREALRRLVHRGLAGARVVVDPAHGPDDPGVTGPGGTREHDVTWEIARRLVARL
                     SARGVHAVLSRGHSTTPPPSERARFANEQGADALVGIGLSGHPTPAATGSSAYYFGSP
                     TFVSEAGELLAGLCQEELVTAGARPDGRCHPVTWTVLRETRMPAIVVEPGFLTSPRDE
                     ARLTSPEGQEAIAEALLRGIARFLEPRPGD"
     gene            complement(8463..9056)
                     /locus_tag="VNU01_12000"
     CDS             complement(8463..9056)
                     /locus_tag="VNU01_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH33383.1"
                     /translation="MFWEAPSGRPGPTPGGEAGKEAWWRAAELEWGPPGKGVYLDDEL
                     AGYVLYGPPEHFPRIRASGRTASDDALLLATLWVAEDHRGGGLGKTLLQVAMREAARR
                     NLRAVEALAVRDDDPPAWRCTTPAGFLEAHGFAVFQDDARHPLLRLDLRQTVRWQEQV
                     GHALESVVSALSRRERLPKPAPDTGLARTGMAKPERP"
     gene            complement(9126..9323)
                     /locus_tag="VNU01_12005"
     CDS             complement(9126..9323)
                     /locus_tag="VNU01_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33384.1"
                     /translation="MPSDPADLARRQPAALLRAVRSDDLAVHHEVADPGPDPLTRAVV
                     GLLLGAAVGAAAALLTRRHEG"
     gene            9516..10763
                     /locus_tag="VNU01_12010"
     CDS             9516..10763
                     /locus_tag="VNU01_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWH33385.1"
                     /translation="MSEIKLDPYLSRYAVRTRGMTASEIRALFAVASRPEIVSLAGGM
                     PFTAALNYDALQAVTQEVLSANGPVALQYGGGQGLPELREQLVEIMRAEGTAAHADDL
                     VVTAGGQQGLDLVAKLFCDPGDVILAEGPTYVGALGAFSAYQADVRHVPMDSQGVIPE
                     ALENALAALRQEGRSAKFFYTIPNHQNPAGVSLSVARRERIVEIAAEHDLLILEDNPY
                     GLIDFKGEQRPSLRTLSPERVIYLGTLSKILSPGIRVGWVAAAGPVRDKLVLLKEAAD
                     LCQSNFTQYVAERWFATQPWQRQVEEFTDLYRERCRAMLDELAATFPPSCTWSEPTGG
                     FFVWATVPAEIDTKELLAKALSERVAYVPGVGFYADGSGRNAMRLSFCFPTAEVIREG
                     VSRLASVLHAELELVQAVYGKRA"
     gene            10817..>11603
                     /locus_tag="VNU01_12015"
     CDS             10817..>11603
                     /locus_tag="VNU01_12015"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWH33386.1"
                     /translation="MSLRSGRRVMEALSDRGHHATLLDLDNELVPTLAKGSFDAAFLT
                     LHGKAGEDGTIQGLLELLGLPYTGPDAVASAIAWDKGVFKGMLGRAGLSTPPWVAVSS
                     EAIRTMGAGQALDRIGASLGGPFVVKPAQGGASMGVRFVPDEGGLTQALVTAFNYSNV
                     ALVERFIAGTEVAVSIIDDEPMPAVEIVPLSGSYDFTARYTAGATEFFAPARLDGATL
                     DRCAELAVAAYRLAGARQITRADMIVDADGQPWLLELDTCPGMT"
BASE COUNT         1505 a         4189 c         4376 g         1533 t
ORIGIN      
        1 gagcgggccg gccgaccccg tgacgggacc gggcagcgcc ccggccgggc acgcgcccga
       61 cggcgcggag cagccggcgc tggcgcgcaa cacggcggtg atggcggccg gcacgctgct
      121 gtcgcgcatc accggcttcg gccgcctggc ggccatgacg gccgcgttcg gcatcgtcga
      181 gggccccttg gcggacgcct acaacaaggc caacgtcgcc ccgaacatcg tctacgagct
      241 ggtgctcggc ggcgtgctgt ccagcgtgct gatcccggtc ctggtggaga cgctgcgccg
      301 agacgaggcc gagcaccgcc acgtcaccaa cacgatcacc accatcgcgg gggtggtgct
      361 gctggcgatc accacggcgg gcatcgccgg ggcgccgtgg cttgccgtgc tgttcacgct
      421 gggagccgac ggtccggcgg cggaggtcgc ggagctgcgg cgggtggtca cgctgttcct
      481 gcgcctgttc ctcccccagg tgctgttcta cgggctcacc acgctgtgga cggcgtacct
      541 gaacgcgcac cggcacttcg ccgcgcccat gttcgcgccc gtgctcaaca acgtcgtcgt
      601 gatcggcgtg ctgctgtggt acggcgccat cgtcgacttc cgcgccgtgg cggaactggg
      661 ccgcctgagc gccgcgcagc tgtggctgct gggcctgggc accaccgccg gcatcctggc
      721 catgacgctg ccgttgtggc cggtgagccg ccgccacggc tgggcgttcc gccccagcct
      781 ggactggcgc cacccgatgg tgcgccgcgt ggggaagctg ggtggctggg cggtgctgta
      841 cgtggcgctc aaccaggtcg gctatctggt ggtgatcgtg ctgaccagcg ccatcccggg
      901 cgacggcgcg ttcgccgcct acgccggcgc gttcgtgttc ttccagctgc cccacggcat
      961 ctacgccgtc agtgtgatga ccgcgctggt cccggggctg gcggaggcgg cctccgccgg
     1021 ggagaccgcg cgctaccggg cccagcttgc cgctggcatc cgcgccaccg ccctgttcat
     1081 cgtgcccgcg gcggtgggcc tggccgtcct gtcggcgccg atcgtgcgcc tgctgctgga
     1141 gcgcggcgcg ttcaccgccg agtcgaccga gctcgtgtcc gcggtgctca ccgcgttcgc
     1201 catcgggctg cccagcttca gcctgttcca gctgttcctg cgcgcccact acgcgctcca
     1261 ggacacccgc accccggtcc tggtcaacgt ggccgccgtg tcgctcaaca tcgccgtcga
     1321 cgtcgcgctg ttcctgctgc tgccggggca atggaaggtc gtcggcctgg cgctgggcca
     1381 cgcggcggcg tacacgctgg gcgcggcgct gttcgcgctg cgcctgcggc gccggatcgg
     1441 cggcctgggc gggcgccaga ccgccggggc gctgctgcgc atcgccgccg cctccgccgg
     1501 catgggcgcc ctggtgtccg cggtggcact ggcgctgcgg gagaccgtgg gcgtggccgg
     1561 attcggcgcg caggcgctgc aggtcgccgc cgccacgggc atcggagcgg tcgcgtacct
     1621 ggcactggcc cggctcgcgg gcgtgcggga gctggacttg ctgaaatcgc tggtgcgcgg
     1681 gcggcgtcat gccgactgac aaggggaggt ccaccatcgg tcggggagga cgcatgcatc
     1741 gccacggcga acacggcggg gtcatcgtgt ccgggctgct gcgcaccgcg gtgtgggtcg
     1801 gcctgttctg cgtgctggcc ttcgacacgg gcggcatcgt gaccaaccgc gtgctgctcg
     1861 acgaggccac ccgcgtggcg gcccgccagg ccgccgacag cctggaccgc agtcgcggcg
     1921 tggcgacggc atccgccgtg cgcgcggcgc gcgaggcggc ggaggacagt gtcgcggacc
     1981 agcccgggat cgaagtcctc gacgtggcgg tggagcgcgg ggaagtgtcc gtgaccgcgc
     2041 gccgccgcgc gcgcgtcctg gtggccgacc ggatcccgcc gctgcgcgac cacgtggagg
     2101 cggagtcgcg cgcctccgcg tcgctcggcg ggccgtgacg ggcgtccccg acgggcgccg
     2161 gggaatgcgt tcgggcgtag actgccgcac tcgtagaccg cacccggggc gaccggaccg
     2221 cccccgccga tcgccgccac ggaggtgagc gggacgccac ccgacgcgcc cggccagccc
     2281 acccagccag accgcgaggc tccgccgcgc gtgctgggcg ggcgttacct gctcgaagag
     2341 cccatcgcca cgggcggcat ggcgatggtg tggcgcgcgc acgacgagct gctggcgcgc
     2401 accgtggcga tcaagatcct gcacgaccac ctcgcggcga acgaggcctt ccgcgagcgc
     2461 ttccgccggg aggccatcgc ggcggccaag ctgacgcatc ccaacgtcgt gggcctgttc
     2521 gacaccgggt cggacgacag ccgggtgtac ctggtgatgg agtacgtcga cggccccacc
     2581 ctgcgggatg tcctgcgcga cctcggcagc ctggagcccg gcgaggcggc cgcgatcggc
     2641 gagaagatcg cccgtgcgct ggcctacgcg cacgagcgcg ggctggtgca ccgcgacgtc
     2701 aagcccgcca acgtgctcat cggcgaggac ggcaccgtca agatcgccga cttcgggatc
     2761 gccaaggccg aggcgggcgc cgacgacctc accacgcccg gcatggtgct cggcacggcc
     2821 gcctacgtgg cgcccgagca ggtccgcggc gaggaggtcg acggccaggc cgaccagtac
     2881 gcgctcggct gcctgctgta cgagatgctc accggccagc agccgttcaa gggtgacacc
     2941 gcggtcgcga cggcggccct gcggctggag cacgacccat tgccaccccg gtcgctgcgg
     3001 gcgggcatcc cgcgcgggct ggacgacgtg gtgctgcgcg ccatggcgcg cgaccccgcc
     3061 gcccgtcatg cctccctggc gcacctggcg gatgcgctgg cgccgttcgc ggccatggac
     3121 gcgaccgccg cgctcacgcc cctggccgac agcgggcggg cggccgcgtg ggcggccggc
     3181 ccggtcgccg acgacgcgcc cacgctgtcg gggacgccgc cgtacgggta cggccagccg
     3241 ctcccgccat cggcgctgcc ctacgccggc tccaccgcgg aggactcgtt cctgcgcagc
     3301 gaggggcgct ggatcgcgcc gatcctgggc ctggtggcgc tggcgggcct gctcgtcggc
     3361 gtgggcctgg ccacgggtgt gctggagccg tcgagcgggt ttcccatccg catcgcgcgg
     3421 gagtccgccc ccggacaggc cggcgagctg atcgcgcccg tgggcatcga cgcgttcgac
     3481 ccgcccccgg gcgacggcag cgagaacgac agcgacctgc ccaagatcct ggacggcgac
     3541 gacgcgacgt cctggcgcac ggacctgtac cgcaactccc cgcagttcgg caacctcaag
     3601 gagggcgtgg gtttctgggt ggacctgggc gcgtcgcgca cactgcactc cgtggcgctc
     3661 aagacgacga ctccgggaat ctcctaccag gtgcgggtgg cggacggccc cgcgcccacg
     3721 gtggagggct ggcgcctggt cggcacggtg gagtcggccg agggcctgga ccaggtgggc
     3781 ttcgccgagc cggtgacggc gcgctacgtg ctggtgtgga tcaccgggaa cctgcaaccg
     3841 cagcgcggcc gcttcgcggc cggcttcgcg gcgatggccg tcaggggcgt ggcgtgacgg
     3901 ggctcccgcg gtgagcgcgg cggacgtcgc cgacgaggtc ctcgtcggcg aatgggcgcg
     3961 cggcgaccag gccgcgttcg acgagctggt gcggcgctac cgccgccgcg tctacggcat
     4021 ctgctaccgc tacttcggcg acgcggccga cgccgaggac gccgcccagg acgcgttcgt
     4081 ggcgctgctg cgcaaggccg gcaccttcca gggcacgtcg agcttctcca cgtggatgta
     4141 ccgggtcacg gtgaacgcct gcaacgacct ggcccgcaag cggggccggc ggccgcagcg
     4201 cacggacacg gacgtctcca ccctggccga cgacctggcc ggcaccgagg acctggtcgg
     4261 ccggcgcgag ctcggcatgg tcctggaggg cgcgctgcgc gagctggacc ccgacacccg
     4321 cgcggccatc gtcctgcacg acgtgcaggg cgtgccctac caggacatcg ccgagcgcct
     4381 gggactcgcg gtggggaccg tcaagtcacg gatccaccgt ggccacgccc ggctcgccgc
     4441 gctcttgaca gaagccgggg gaaccctgcc ggacggctcg gcctccaacc ctgcgaagcc
     4501 cacatctgga cacgcatgaa cgactaccgc ccacccgacg cgcaaggccc ggcccagccg
     4561 gagccggacg aggcgctgtc ggcgtacctg ctcggcgatc tcgaggacgg ggccgcggcg
     4621 gcgttcgagg cgtggctcgc ggccgacgcg gggctgcgcg cccggctcga cgcgctcgcc
     4681 ggcgcgctgg cggccctggg cggcgtggac gacgtcgacg agccggacgg cttcgcggaa
     4741 cggctgggcg cccggctggc ggcggagcgc gacgtggccg atctgagcgc ccgccgcgag
     4801 cggcggcggc gctggagcgc ggtgctgacc gcggccgccg gcgtggtggt gctggcggtg
     4861 gccggcctcc aggtgaccac cggcgggccg ccggcgcccc gggacgagcg cgtggcgcgc
     4921 gagatggcgg ccggagacga tgccgccgtg gagcaggcgg cgggcgaggc gctgctggag
     4981 agcgacggca tggagctgga gtccggggcg ggcagcgccg acgcgtaccg cgcccccgag
     5041 ccgatggcgg acggggacgg cggtgccgcc ggcgtcgagg agaccgcggc ggacggcccg
     5101 gccgacagcg ccggcggcgg ttccggcggc gccccgtcga tggccgcggc ggacgacggc
     5161 cagggcccgg cgatcgtgga cgagggcgca tcgctggacg acgacgacca gttgcgcgcg
     5221 cgctacgagg gggcgcccga gtccgagcgg ttccgcggcc ttccccggga ggatgccgcc
     5281 gaggtcgccg cccgccacgc cgaccaggtc cgcgccgcgg acgcgttcgc gtccggcgcg
     5341 cggcccgacc gctgcctgga cgaggtcggc gccgaacgcg acggccgcat cgtcaccgtg
     5401 gtggagcggc tgcgctggca gggcggggag gccctggcgt acgtcctggc cctgccgtcg
     5461 gagacgtcgg cgaccgtgga ccgcttcgag gcctgggtca tggggccgca gggctgcgac
     5521 atcctgggcc gggtgtcctg gcggtagcat cggacccaga ccgcacgcca caaggagccg
     5581 caccgcaatg gccgacgccg ccgacctccc cgccgagccc gtccgcgagg tgctcatcat
     5641 cggctccggc cccgcgggcc tgaccgccgc gatctacgcg gcccgcgcca atctcgagcc
     5701 cctcatgatc gagggccgcg agcccggcgg gcagctcatg ctcacgaccg aggtcgagaa
     5761 cttcccgggc tttcccaccg gccgcatggg cccggagctc atggcggaca tgcgggcgca
     5821 ggccgagcgc ttcggtacgg agatcctcac ggcagacgtg gacgaggtcg acctggcgag
     5881 cggctcgtcg ttcacggcga cggtcgggga caccgtctac cgggcccgca ccgtgatcat
     5941 ctcgaccggt gcgacggccc gctggctggg tctgcccaac gagcagcggc tcatcggccg
     6001 cggcgtgtct agctgcgcca cctgcgacgg cttcttcttc cgcgaccgcg agctcgtggt
     6061 ggtcggcggc ggcgactccg ccatggagga ggcgacgttc ctcacgaagt tcgccagcaa
     6121 ggtcaccgtg gtgcaccggc gcgacgagct gcgcgcctcc aagatcatgc aggagcgcgc
     6181 cctcaagaac gacaagatcg agttcctgtg gaacgccgag gtggtcgagg tcctgggcgc
     6241 cgacgtggtg gagggcgtcg tgctgcgcga cacgctcagc ggcgagcagc gcgacttccg
     6301 caccgacggg ctgttcctgg ccatcgggca cgaccccacc accgcgctgt tccggggcca
     6361 gctcgacctg gacgacgacg gctacatcct ggtctccgag ccgcgcacgg agacgagcgt
     6421 gcccggggtg ttcgccgccg gcgacgtggt cgaccgcatc taccgccagg ccatcaccgc
     6481 cgcgggcatg ggctgcaagg ccgcaatcga cgcggagcgc tggctggagg cacaggcgta
     6541 aagatcaacg gggccaggaa gcgatccgct ttacgcctgt agccttgccg tgcgcgccgg
     6601 ccccgagccg cttgccgcca aagtcgggat ctgggcgatt cgatttgcgc gccgcgcgga
     6661 actggcttat aaatcgcatc cgcaaaactt gatatgatgt aggtctgttc gtccggcgca
     6721 tctgggaaca cccggtgagt gcgacgacga gcggcacaca gatccgcggt tcggctgtcc
     6781 ccccgtcatc cgaaccgcgc gcgaggggcg ctacggcgcc cctcgcttct tttcgcccgg
     6841 ccaagggaaa taggctgggg ggaataggct gcacgtcagc gggcgttgcc cggcggagcc
     6901 ggcgacacgg cgctggaccg cggcaggacc gcctcacgga ctcgaaggag ccacagacat
     6961 gggcacaacc aagtcggtca ccgacgccga gtgggaatcg gaggtcctca agtcggacct
     7021 gcccgtcctc gtcgacttct gggccgagtg gtgcgggccg tgccggatgg tgtcacccgt
     7081 ggtggaggag atcgccggcg agaaggccgg tgccctcaag gtcctcaagc tgaacgtgga
     7141 cgacaacccc gagaccgccc gcaagtacaa ggtcatgtcc atccccacgc tcatggtctt
     7201 ccagggcggc gacgagaaga agcgcctggt cggtgcccgc ggcaagagcc agctcctcca
     7261 ggaagtggac gagttcctgt cgtaacggcg cttcccggcc gcccggccac ggccgtgcgg
     7321 tgacgtgcgg gactacactg cgccgccgtg agtgaccccc agcgcacccc cccgatccgc
     7381 ctcggtgacg agggcgcgcc cgtcagcgac ctccagcgcc ggctcgtccg cctgggcgag
     7441 caggacctgg aggtcgacgg ccgtttcggg ccggtcaccc tggaagcggt gcgccgcttc
     7501 cagcgcgagc acgggctggc cgccgacggc gtggtggggc cggagacctg gcggcgcctc
     7561 gtggaggcag gctgggcgct cggcgaccgg ctgctgtacc agcgccgcgt gatgctgcgc
     7621 ggcgaggacg tgctggagct gcagcagcgg ctcagccgcc tgggcttcga cgcgggaccg
     7681 gaggacggca tcttcgggcc tctcctgcac gccgccgtgg aggagttcca gcgcaacgcg
     7741 ggcctggagg tcgacggcgt ggccggtccc gccaccgtgg cggcgctgcg gcgcctgcac
     7801 cgcgaccacc agcgcggcgg ccatgtggcg cgcctgcgtg agcgggaggc cctgcggcgg
     7861 ctcgtccacc gcggcctggc gggcgcgcgc gtggtggtgg accccgccca cgggccggac
     7921 gacccgggcg tgaccggtcc cggcgggacg cgcgagcacg acgtcacctg ggagatcgcc
     7981 cggcgcctgg tcgcgcggct gtccgcccgc ggcgtccacg ccgtgctgtc gcgcggccac
     8041 tcgaccaccc cgccgccatc cgagcgtgcc cgcttcgcca acgagcaggg cgcggacgcg
     8101 ctcgtcggca tcggcctgtc cgggcacccc acgcctgccg cgaccggcag cagcgcctac
     8161 tacttcggct cgccgacgtt cgtgtcggag gccggggagc tgctggcggg gctgtgccag
     8221 gaggagctgg tgaccgccgg cgcgcgcccg gacgggcgct gccatccggt gacgtggacg
     8281 gtcctgcgcg agacgcgcat gccggccatc gtggtggagc cagggtttct gacgtccccg
     8341 cgggacgagg ctcgcctgac cagccccgaa gggcaggagg cgatcgccga ggcgctcctg
     8401 cgaggcatcg cgcgcttcct ggagccgcgg ccgggcgact gaccggtccg ggagcggccg
     8461 cgtcagggcc gctcgggctt cgccatgcct gtccgggcca gtccggtgtc gggggccggc
     8521 ttgggcagcc gctcgcggcg gctgagcgcg ctcaccacgc tctccagggc atggcccacc
     8581 tgctcctgcc agcgcaccgt ctggcgcaga tccagccgca gcagcgggtg gcgggcgtcg
     8641 tcctggaaga ccgcgaagcc gtgcgcctcc aggaagccgg ccggggtcgt gcagcgccag
     8701 gcaggcgggt cgtcgtcgcg cacggccagc gcctccaccg cgcgcaggtt gcggcgggcg
     8761 gcctcgcgca tggccacctg cagcagggtc ttgcccagcc ccccgccccg gtggtcctcc
     8821 gcgacccaca gggtggccag cagcagggcg tcgtccgagg cggtgcgccc gctggcgcgg
     8881 atgcggggga agtgctccgg cgggccgtac agcacgtagc cggcgagctc gtcgtccagg
     8941 tagacccctt tgccgggcgg gccccactcc agctccgccg cgcgccacca cgcctccttg
     9001 ccggcctcgc cgccgggcgt gggaccgggc cggcccgacg gggcctccca gaacacgcac
     9061 gaccggcagg gtccgggcag gtccccgggc cggctgatct cccggatgcg cggtcccagc
     9121 accgctcagc cctcgtggcg gcgcgtcagg agcgcggcgg cggcgccgac ggccgcgccg
     9181 agcaggaggc ccacgacggc ccgcgtcagg gggtccggcc ccgggtcggc cacctcgtga
     9241 tggaccgcca ggtcgtcgga ccggaccgcg cgcagcagcg ccgccggctg tcgccgagcc
     9301 aggtccgcgg ggtccgacgg caccgtttcc tcctgtgcgg gcgcgctctg gccgcagtgt
     9361 agccagcacc gcaccgcccc attccgggtg cccgggccgt gacgccgccc gaggagcgaa
     9421 cgattcacgc cgaaggcgtg actgagcgac gagggatggc ggcacgcccg gagagacgac
     9481 agggcgatgc tacgctgcga cgcggaggac ccgccatgag cgagatcaag ctcgacccct
     9541 atctgtcgcg ctacgcggtg cgcacgcgcg gcatgaccgc ctcggagatc cgggcgctgt
     9601 tcgcggtggc cagccgcccc gagatcgtgt cgctggccgg cgggatgccg ttcacggcgg
     9661 cgctgaacta cgacgccctc caggcggtga ctcaggaggt cctgagcgcg aacggccccg
     9721 tcgccctgca gtacggcggc gggcagggcc tgccggaact gcgcgagcag ctcgtggaga
     9781 tcatgcgcgc cgagggcacg gcggcgcacg cggacgacct cgtggtcacc gcagggggcc
     9841 agcagggcct cgacctggtg gcgaagctgt tctgcgaccc gggcgacgtc atcctggccg
     9901 agggccccac ctacgtcggc gcgctggggg cattctcggc atatcaggcc gatgtgcgtc
     9961 atgttcccat ggattcccag ggagtgatcc cggaggcgct cgagaatgcg ctggccgcgc
    10021 tgcgccaaga gggccgcagc gccaagttct tctacacgat cccgaaccac cagaacccgg
    10081 ctggcgtctc gctgtccgtg gctcgccgcg agcgcatcgt ggagatcgcc gcggagcacg
    10141 acctgctgat cctggaggac aacccctacg ggctgatcga cttcaagggt gagcaacggc
    10201 cgtcgctgcg cacgctgtcg ccggagcgcg tgatctacct ggggaccctg tccaagatcc
    10261 tgtcccccgg catccgcgtc ggctgggtcg cggcagcggg cccggtgcgc gacaagctgg
    10321 tgctgctcaa ggaagccgcg gacctctgcc agtccaactt cacccagtac gtcgcggagc
    10381 gctggttcgc gacgcagccg tggcagcgcc aggtcgagga attcaccgac ctgtaccgcg
    10441 agcgctgccg cgcgatgctc gacgagctcg cggccacgtt cccgcccagc tgcacgtgga
    10501 gcgagcccac cgggggcttt ttcgtctggg cgaccgtgcc ggcggagatc gacaccaagg
    10561 agctcctggc caaggcgctg agcgaacgcg tggcgtacgt gcccggggtc gggttctatg
    10621 ccgacggcag cgggcgcaac gccatgcggc tgtcgttctg cttccccact gcggaggtca
    10681 tccgcgaggg tgtcagccgc ctcgccagcg tgctgcacgc ggagctcgaa ctggtccagg
    10741 ccgtctacgg aaagcgggcg tgaccatggc gttcaaggtt gtgattctct ctggaggcct
    10801 gagtctggag cgcgacgtga gcctgcgctc cggccggcgc gtgatggagg cgctgtcgga
    10861 ccgcggccac cacgcgacgc tgctggacct ggacaacgag ctggtcccca ccctggccaa
    10921 gggctcgttc gacgcggcct ttctcaccct gcacggcaag gcgggcgagg acggcacgat
    10981 ccaggggctg ctggagctgc tgggcctgcc ctacaccggc ccggacgccg tggcgtcggc
    11041 gatcgcctgg gacaagggcg tgttcaaggg catgctgggg cgcgccgggc tgtccacacc
    11101 gccctgggtg gcggtgtcct ccgaggcgat ccgcaccatg ggcgccgggc aggcgctgga
    11161 ccgcatcggg gcgtcgctcg gcgggccgtt cgtggtgaag ccggcgcagg gcggggcctc
    11221 catgggcgtc cggttcgtcc ctgacgaagg tgggctgacg caggcgctgg tgaccgcctt
    11281 caactactcc aacgtggcct tggtggagcg gttcatcgcc gggacggagg tggccgtgtc
    11341 gatcatcgac gacgagccca tgcccgcggt ggagatcgtg ccgctcagcg ggagctacga
    11401 cttcaccgcc cggtacaccg cgggcgccac ggagttcttc gccccggcgc gactggacgg
    11461 cgccacgctg gaccgctgcg ccgagttggc cgtcgcggcg taccggcttg ccggcgcgcg
    11521 gcagatcacc cgtgccgaca tgatcgtgga cgccgacggt cagccgtggc tgctggagct
    11581 ggacacgtgc cccggcatga cgg
//
LOCUS       DATMKC010000411         6805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_158538, whole genome shotgun sequence.
ACCESSION   DATMKC010000411 DATMKC010000000
VERSION     DATMKC010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158538"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VNU01_12020"
     CDS             <1..267
                     /locus_tag="VNU01_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWH33387.1"
                     /translation="VAYSLWFGAFDRLPASTVSFLSLLSPVVATAIGYAAGQHLSGTQ
                     LAGAAIAVASVGAGQYFAQRDAAGAAPVPAGPGPAPLAAARPVA"
     gene            complement(274..432)
                     /locus_tag="VNU01_12025"
     CDS             complement(274..432)
                     /locus_tag="VNU01_12025"
                     /inference="COORDINATES: protein motif:HMM:NF040003.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HWH33388.1"
                     /translation="MIVLGVILLLLGALFEVPAVVTTIGIILIVVGALMMLLGAMGRP
                     VGPRRYYW"
     gene            619..996
                     /locus_tag="VNU01_12030"
     CDS             619..996
                     /locus_tag="VNU01_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33389.1"
                     /translation="MPQTDGAGGYRSDPSATAPTAAPDDAPPRTQPFMPAEGPAAARG
                     ERAERDEGRDLLRDPVAAMSEPDNRSKLWLATAALTALNTLLLLGLLFADGADSGPQP
                     VMVDGQPCLVVEGEDAGTLFCRR"
     gene            complement(1001..1399)
                     /locus_tag="VNU01_12035"
     CDS             complement(1001..1399)
                     /locus_tag="VNU01_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33390.1"
                     /translation="MTSRCAICGKEVAQSGPARQWRHLNEFYAKADHPADPASKTYVA
                     PKAAPPPPAKKAEEPKPKRDGNKPKSLAELRRRLQQGTAEAQPDDSLARRRRVAALDA
                     GDTGPTDEELAARRLRLMRLQAAAEEEKRR"
     gene            1584..2057
                     /locus_tag="VNU01_12040"
     CDS             1584..2057
                     /locus_tag="VNU01_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33391.1"
                     /translation="MATKDEQIYQVAVERQKAAQAAGNYDLADLPGDLGTPAAAARVG
                     KTPKQDKVLKGGRALSAVARLTPGDALAVFGRPESRWAMAYWRRTGGTADMTELLSYA
                     RQLIGMTPSGDLVVCLCGHAGQGPCIPLWAPREEVSLTVQPNDLVLRFDDLVEDQ"
     gene            2054..2908
                     /locus_tag="VNU01_12045"
     CDS             2054..2908
                     /locus_tag="VNU01_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWH33392.1"
                     /translation="MSQAASGPAEPEPGADAGSPRELDPPPGWLDRAHELALGLADLA
                     DDMALSRFGSSVPAETKPDGSPVTEVDRMVESALRQRIAAVFPEHAFLGEETGGTLDH
                     NRPTWVIDPIDGTKNFMRGVPVFACLIGVVWHGHGVLGVASAPAMGERWDAAHGRGAR
                     RNGQPVHVSAVRRLADASVLHGALEWYRDPALWRVLEHLADHAWTTRGFGDFWMHLLV
                     AGGMADAAFETGLKPWDIAALQAIVCEAGGSLTAFDGGDALAGADGAVLTTNGLLHAE
                     LSALLAAG"
     gene            complement(2905..3231)
                     /locus_tag="VNU01_12050"
     CDS             complement(2905..3231)
                     /locus_tag="VNU01_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33393.1"
                     /translation="MSAKSEYDAAYFTLLRAIEERDGLLRYREFLEAERARLDQVSAA
                     TREGGEAVPERIRRPVDRTAKQLLEAVGRRRVVVLDELGRMDKRLAQAEAFVEECEAD
                     VASLRP"
     gene            complement(3228..4658)
                     /locus_tag="VNU01_12055"
     CDS             complement(3228..4658)
                     /locus_tag="VNU01_12055"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWH33394.1"
                     /translation="MSGRFDDVRAVLDDRMDTHRQDLEALVRLPSVSAAGFDPGQVRR
                     CAEAVRELLAARGLDNAVLLEVVGTHPAVYADWLHAGPDAPTVLLYAHYDVQPIGDPA
                     AWSSPAFTPTEREGRLFGRGAADDKAGILAHVAAVDAWLRARGALPVNLKVIIEGEEE
                     IGSEHLMQFLDAHGELLRADVVVVTDLENWAVGTPSITYLLRGLIDAVVQVRALDHTL
                     HSGMYGGPVPDALHALVKLLARLTDERGDVAIPGFADDVRPLSPAERARLDALDFDEA
                     HFRADAGVPDGVELLGDPSTPVLERLWARPALSILGLDAPAVALSSNTLSPAAAARVS
                     IRLAPGQDPARATKLLADFLREQAPWGVRVEVTPGWCAGAWSEEPTGPAFEACARALE
                     AAYGAPAVFTGVGASLPFVEAVTSALDGVPALLLGVEDPDSRAHGVDESLHLGDFRNA
                     CLGEAYLLGELAALGRASVRGEQARA"
     gene            4745..5161
                     /locus_tag="VNU01_12060"
     CDS             4745..5161
                     /locus_tag="VNU01_12060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWH33395.1"
                     /translation="MDERFALRLSPRYMEVDQQGVVFNAWYLVWFDEAFTAWMRHVGV
                     GYDELVGRGVDVQNVHAELDYRSGVRWGEEVGVEVDVVRVGGKSFTLGYTVRRGGEAC
                     VEGSIVYAVVATDGSGALEIPADLRAILEEHQRQAA"
     gene            5158..5805
                     /gene="aat"
                     /locus_tag="VNU01_12065"
     CDS             5158..5805
                     /gene="aat"
                     /locus_tag="VNU01_12065"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWH33396.1"
                     /translation="MTPSRFPDPREAPGDQPLGYGGDLAPETLLDAYGHGIFPWPDAD
                     GTLWWWSPDPRAVLPLDGFHVSRSLRRTLRAGRFTLTVDHAFTSVVRACADRPEGTWI
                     VPAYARAFQRMHELGHAHSVEAWDAEGRLAGGVYGLALGGAFFAESMFHRATDASKVA
                     LFHLVQRLRDGGFTLLDVQLPTPHLASLGAVTISREDYLDRLTDALGRSGTTFAR"
     gene            6250..>6805
                     /locus_tag="VNU01_12070"
     CDS             6250..>6805
                     /locus_tag="VNU01_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33397.1"
                     /translation="MVAELDIVLRLEPVREALADLERTKAVSRLYDRDASLWSGDASV
                     QAKIRDRLGWLDAATGNDDWRKQLTDLAAAAQADGLSHVVLAGMGGSSLAPEVFSLVF
                     RGHARGATLTVLDSTHPEAVRAALDETDLAHTLVVVASKSGSTEETACFAARAAALVP
                     SPAHLLAITDPGSALQRQAEADGWR"
BASE COUNT          863 a         2566 c         2487 g          889 t
ORIGIN      
        1 gtggcgtact cgctgtggtt cggcgcgttc gaccgcctgc ccgcgtccac ggtgtcgttc
       61 ctcagcctgc tgagccccgt ggtggccacc gccatcggct acgcggcggg ccagcacctg
      121 agcggcacgc agctcgccgg ggcggccatc gcggtcgcgt cggtcggcgc cgggcagtac
      181 ttcgcccagc gggacgcggc gggggccgcg cccgtcccgg caggccccgg gccggcgccg
      241 ctggcggccg cccggcccgt ggcctgagtc aggtcaccag tagtagcggc gcggccccac
      301 cggacggccc atcgcgccga gcagcatcat cagcgcgccg acgacgatca ggatgatccc
      361 gatggtcgtc accacggcgg ggacttcgaa caaggcgccg agcagcagca ggatcactcc
      421 gaggacgatc atctcttctg gcctccagcc ggcgcgtgcg cgccgcatcg acggcatcgt
      481 tgtcgtgcgt cgacattccc tccgcccggt cgccgaaacg caaaggcggg gttcggccgg
      541 ccaggcgtgg accggccccg ccgcgggcat gcacagcgca tcgacgcctt ttccgcgacg
      601 tccactggag gacttccgat gccacagacc gacggtgcgg gcggttaccg cagcgatccc
      661 tccgccacgg cgcccacggc ggcgccggac gacgcgccgc cgcgcacgca gccgttcatg
      721 cccgccgagg gacccgccgc cgcccggggc gagcgcgcgg agcgcgacga gggccgcgac
      781 ctgctgcgcg accccgtggc ggccatgagc gaaccggaca accgctccaa gctgtggctg
      841 gcgacggcgg cgctcaccgc gctcaacacg ctgctgctgc tcggcctgct gttcgccgat
      901 ggcgccgaca gcggcccgca gcccgtgatg gtcgacggcc agccgtgcct ggtggtggag
      961 ggcgaggacg ccggcacgct gttctgccgg cgctgaggcc tcagcggcgc ttctcctcct
     1021 cggcggccgc ctgcaggcgc atgagccgca gcctgcgggc cgccagctcc tcgtcggtgg
     1081 gcccggtgtc gccggcgtcc agggcggcca cgcgccgccg tcgcgccagg ctgtcgtccg
     1141 gctgcgcctc ggccgtgccc tgctgcagcc ggcggcgcag ctccgcgagc gacttgggct
     1201 tgttcccgtc gcgcttgggc ttgggctcct ccgccttctt ggccggaggg ggcggcgccg
     1261 ccttgggcgc cacgtaggtc ttggaggcgg ggtccgccgg gtggtcggcc ttggcgtaga
     1321 actcgttgag gtgacgccat tggcgcgcag gcccggactg cgcgacctcc ttgccgcaga
     1381 tcgcgcaccg gctggtcatc gagaatcgtg gcatgacgtc caccgacccc tcgaacggtg
     1441 aaacggcgcg tgtgtttcat gttacgccga gtgagcgcac tccatgacga accggatccg
     1501 tccgggtgcg cgcacccgcc ccgccggtac actgcccgga gccgtcgcag tcgcgggtgt
     1561 cgagcagagg atcctgaagc gcattggcca ccaaggacga gcagatctat caggtcgcgg
     1621 tggagcggca gaaggccgcg caggccgcgg gcaactacga cctggcggat ctgcccggcg
     1681 acctcggcac tcccgccgcg gcggcgcgcg tcggcaagac gcccaagcag gacaaggtcc
     1741 tcaagggcgg ccgcgcgctg tcggccgtgg cccggctcac ccccggtgac gcgctggccg
     1801 tgttcggccg gcccgagtcg cgctgggcga tggcctactg gcggcgcacc ggcggcaccg
     1861 ccgacatgac cgagctgctc agctacgcgc gccagctcat cggcatgacg cccagcggcg
     1921 acctggtggt gtgcctgtgc gggcacgccg gccagggccc gtgcatcccg ctgtgggcgc
     1981 cgcgcgagga agtcagcctg acggtccagc ccaacgacct ggtgctgcgc ttcgacgacc
     2041 tcgtcgagga ccagtgagcc aagcggcatc cgggccggcc gagccggagc ccggagcgga
     2101 cgccggctcg ccacgcgagc tggatccgcc acccggctgg ctggaccggg cgcacgagct
     2161 ggccctgggg ctggcggacc tcgccgacga catggcgctg tcgcgcttcg gctcgtcggt
     2221 gcccgccgag accaagcccg acggcagccc ggtcaccgag gtcgaccgca tggtcgagtc
     2281 ggccctgcgc cagcgcatcg ccgcggtgtt ccccgagcac gcgttcctgg gggaggagac
     2341 cggcggcacg ctggaccaca accggccgac ctgggtgatc gaccccatcg acgggaccaa
     2401 gaacttcatg cgcggcgtgc cggtgttcgc ctgcctcatc ggcgtggtgt ggcacggcca
     2461 cggcgtgctg ggcgtggcca gcgccccggc catgggcgag cgctgggacg ccgcgcacgg
     2521 caggggagcg cggcgcaacg gccagccggt gcacgtcagc gcggtgcgcc gactggccga
     2581 cgccagcgtg ctgcacggcg cgctggagtg gtaccgcgac ccggcgctgt ggcgcgtgct
     2641 ggagcacctg gccgaccacg cctggaccac gcgcggattc ggcgacttct ggatgcacct
     2701 gctcgtggcg ggcggcatgg ccgacgcggc gttcgagacg ggcctcaagc cgtgggacat
     2761 cgcggcgctg caagccatcg tgtgcgaggc cggcggcagc ctcaccgcct tcgacggcgg
     2821 cgacgcgctc gccggcgccg acggcgccgt gctgaccacc aacggcctgc tgcacgccga
     2881 gctgtccgcg ctgctcgcgg cgggctaggg gcgcagggag gcgacgtccg cctcgcactc
     2941 ctccacgaac gcctcggcct gcgccagccg cttgtccatg cggccgagct cgtccagcac
     3001 caccacgcga cggcgtccca cggcctccag cagctgcttg gcggtgcggt ccaccggccg
     3061 gcggatgcgc tccggcaccg cctcgccgcc ctcgcgcgtc gccgcgctga cctggtccag
     3121 gcgcgcccgc tcggcctcca ggaactcccg gtagcgcagc agtccgtcac gctcctcgat
     3181 cgcgcgcaac agcgtgaagt aggcggcgtc gtactcggac ttggcgctca tgcgcgtgcc
     3241 tgttcccccc gcacgctcgc gcgacccagc gcggccagct cgcccagcag gtaggcctcg
     3301 cccaggcagg cgttgcggaa gtcgcccagg tgcagcgact cgtcgacgcc gtgcgcgcgg
     3361 ctgtcggggt cctccacgcc gagcagcagc gccggcacgc cgtccagcgc gctggtcacc
     3421 gcctccacga acggcagcga cgcgccgacg ccggtgaaca cggccggggc gccgtaggcg
     3481 gcctcgagcg cgcgggcgca cgcctcgaac gcggggccgg tgggctcctc cgaccacgcg
     3541 cccgcgcacc agccgggcgt gacctcgacc cgcacgcccc acggcgcctg ctcgcgcagg
     3601 aagtccgcga gcagcttggt ggcgcgcgcc gggtcctgcc cgggcgcgag gcggatgctg
     3661 acgcgcgcgg ccgcggcggg cgacagggtg ttggacgaca gcgccaccgc gggcgcgtcc
     3721 agtcccagga tcgacagcgc ggggcgcgcc cacagccgct ccagcaccgg cgtggacgga
     3781 tcgcccagca gctccacccc gtccggcacg ccggcgtcgg cgcggaagtg cgcctcgtcg
     3841 aagtccagcg cgtccaggcg ggcgcgctcg gcgggagaca ggggccgcac gtcgtcggcg
     3901 aagccgggga tcgccacgtc gccgcgctcg tcggtgagcc gtgcgagcag cttcaccagc
     3961 gcgtgcagcg cgtcgggcac cggcccgccg tacatgccgg agtgcagcgt gtggtccagc
     4021 gcgcgcacct ggaccaccgc gtcgatcagc ccgcgcagca ggtaggtgat cgacggcgtg
     4081 ccgaccgccc agttctcaag gtccgtgacc accaccacgt ccgcgcgcag cagctcgccg
     4141 tgggcgtcca ggaactgcat caggtgctcg ctgccgatct cctcctcgcc ctcgatgatc
     4201 accttgaggt tcaccggcag cgcgccgcgg gcgcgcagcc acgcgtcgac cgccgccacg
     4261 tgcgccagga tccccgcctt gtcgtccgcg gcgccccggc cgaacaggcg tccttcgcgc
     4321 tccgtcggcg tgaacgcggg ggaggaccac gccgccgggt cgccgatggg ctgcacgtcg
     4381 tagtgggcgt acagcaggac cgtgggcgcg tccgggcccg cgtgcagcca gtccgcgtag
     4441 acggcgggat gcgtgccgac cacctccagc aggaccgcgt tgtccagccc gcgcgcggcg
     4501 agcagctcgc gcaccgcctc ggcgcagcgg cggacctggc cggggtcgaa gccggcggcg
     4561 ctcacgctgg gcaggcgcac cagcgcctcc aggtcctgcc ggtgggtgtc catgcggtcg
     4621 tccagcaccg cccgcacgtc gtcgaaacgc ccgctcaccg tgatcctttc cgcccaggcc
     4681 cgcgcgcggc cgcgggagcg ccatggtagt cacgccgtcg cagtcgtggt ctagccttgc
     4741 gcgcatggac gagcggttcg cgctgcggct gtcgccgcgc tacatggagg tcgaccagca
     4801 gggcgtggtg ttcaacgcct ggtacctggt gtggttcgac gaggcgttca ccgcgtggat
     4861 gcgccacgtc ggcgtcggct acgacgagct cgtgggccgc ggcgtcgacg tgcagaacgt
     4921 gcacgcggag ctggactacc gctcgggcgt gcgctggggc gaggaggtcg gcgtcgaggt
     4981 cgacgtcgtg cgcgtcggcg gcaagagctt cacgctcggc tacacggtgc gccgcggcgg
     5041 ggaggcctgc gtggaaggct ccatcgtcta tgccgtggtg gccacggacg gctccggcgc
     5101 gctggagatc cccgccgacc tgcgcgcgat cctggaggag caccagcggc aggcggcgtg
     5161 acgccgagcc gcttcccgga cccgcgtgag gcgccggggg accagcccct gggctacggc
     5221 ggcgacctgg cgccggagac gctgctggac gcctacggcc acggcatctt cccgtggccg
     5281 gacgccgacg gcacgctgtg gtggtggtcg ccggacccgc gcgccgtgtt gccgctggac
     5341 ggcttccacg tgtcgcgctc cctgcgccgg accctgcgcg ccgggcggtt cacgctcacg
     5401 gtggatcacg cgttcacaag cgtcgtgcgg gcctgcgcgg accgccccga gggcacctgg
     5461 atcgtcccgg cgtacgcgcg cgcgttccag cgcatgcacg agctgggcca cgcgcacagc
     5521 gtcgaggcgt gggacgccga gggccgcctg gccggcggcg tgtacgggct ggcgctgggc
     5581 ggcgcgttct tcgccgagtc gatgttccac cgggccaccg acgcgtccaa ggtggcgctg
     5641 ttccacctgg tgcagcggct gcgtgacggc gggttcacgc tgctcgacgt gcagttgccg
     5701 acgccgcacc tggcgtcgct cggcgccgtg acgatcagcc gcgaggacta ccttgaccgc
     5761 ctcaccgacg ccctcggccg ctccgggacc actttcgccc gatagcggcc acaagtggtc
     5821 ccgcagcgtg ggtgcgcggg tgggggcggc cactcccgcc gtgcccccat aagcgacatt
     5881 atgtcgacta agccaggagg gtggcccggg gcatgggaaa gccctccggt cggtggcggt
     5941 ggtacccacc ccgcctgccc taccggaggg ctctcacccc ccacagctgc aaaccgttga
     6001 gcggaggtcg cggatgacgc cccggcgcga ggtcccgccc cacaactccg cgacgctggg
     6061 ccggccaccc gcacgaaacc gggcctgtcg cgatccgttc cctcgtgccg gaggcgccat
     6121 cctcgccgcc ccctacggcc attgatacgg acaagctcat cgccgcctaa cgtccgattt
     6181 cgtaaggctc ggacggcgtc cacgcgggta gcctgcccag cgccggaaca acgagtcgtg
     6241 gaggttggcg tggtcgcaga gctggacatc gtcctgcgcc tggagccggt tcgcgaggcg
     6301 ctcgcggacc tggagcgcac caaggcggtg tcgcgcctgt acgaccgcga cgcctcgctg
     6361 tggtccggcg atgcttcggt ccaggcgaag atccgcgacc ggctcggctg gctggacgcg
     6421 gccaccggca acgacgactg gcgcaagcag ctcaccgacc tcgccgcggc cgcccaggcg
     6481 gacggcctgt cgcacgtcgt gctggccggc atgggcggct ccagcctcgc gcccgaggtg
     6541 ttctcgctgg tgttccgggg gcacgcgcgc ggcgccacgc tcaccgtcct ggactccacg
     6601 catccggagg ccgtccgcgc cgcgctcgac gagaccgacc tcgcgcacac cctggtcgtg
     6661 gtggccagca agtccggcag cacggaggag accgcctgct tcgccgcgcg cgccgcggcg
     6721 ctggtcccct cccccgccca tctgctggcc atcaccgacc ccggctcggc gctgcagcgc
     6781 caggccgagg ccgacggctg gcgcg
//
LOCUS       DATMKC010000412         4485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_9162, whole genome shotgun sequence.
ACCESSION   DATMKC010000412 DATMKC010000000
VERSION     DATMKC010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9162"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VNU01_12075"
     CDS             complement(<1..315)
                     /locus_tag="VNU01_12075"
                     /inference="COORDINATES: protein motif:HMM:NF023331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33398.1"
                     /translation="MTKRPSTPTRVAGDVAATASAVAPAENRSVAEQISHWARIGMLV
                     ERAGSVANRRVLAASAGRAPFSSLDADERTAAHALVDARLAELAAAQSFGAAASSARR
                     AST"
     gene            complement(439..514)
                     /locus_tag="VNU01_12080"
     tRNA            complement(439..514)
                     /locus_tag="VNU01_12080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Pro,seq:ggg)
     gene            631..1236
                     /locus_tag="VNU01_12085"
     CDS             631..1236
                     /locus_tag="VNU01_12085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWH33399.1"
                     /translation="MRRDFDWGARKDRPAVVHDKVLTVANGISLARLAGLPLFVWLMF
                     GPQAYGWAWFTLFVVGSTDFLDGYVARRFDQVTKLGKLLDPLIDRGLMATVAISLLAA
                     GMLPWFFVAAILGRDLLILLGTLAFLRAVPEIAVTRTGKFATACLLIGLPGFLLGHMD
                     WPARQVFLVLAWGYTTVGVAGYWIAGLQYARIVRGLRGSGA"
     gene            1233..2063
                     /locus_tag="VNU01_12090"
     CDS             1233..2063
                     /locus_tag="VNU01_12090"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HWH33400.1"
                     /translation="MSERLAGRQRDPAEALGLHRDVVSDYEGLDTARWTRPWSHRTVL
                     VLTVAGALLAGFLLSAALRAGRTVALEQDVRRTELIGLIEERRAHTQRLSAQLEALRR
                     DVDAAEAEAAAGVPALRARLAELEVAAGLTALRGPGLRVELADASGTCATGRTEDCQI
                     QDTDLQLAVNALFAVGAEAVAVNGERVIATTAIRSAGRSVLVNYEVLASPYVIEAIGD
                     PEGLRRRFAESAVASDFTVWKDVYGLGFSIQQADELTLPPYDGGLRLRVAAPAGEALP
                     "
     gene            2060..2392
                     /locus_tag="VNU01_12095"
     CDS             2060..2392
                     /locus_tag="VNU01_12095"
                     /inference="COORDINATES: protein motif:HMM:NF018632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HWH33401.1"
                     /translation="MIPLIALLVGAILGLVFQPTVPAALAPYLPVAVVAALDAIFGAV
                     RARLDGVFSERIFLVSFLTNVVLAVFLVYLGDQLGVGSDLSTAVVVVLGVRIFQNLAS
                     IRRHLFRA"
     gene            2389..3114
                     /locus_tag="VNU01_12100"
     CDS             2389..3114
                     /locus_tag="VNU01_12100"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HWH33402.1"
                     /translation="MSATSAPALRGPLMRVGVALACGLLGFLLVAQVRATESVEDRLA
                     GEREEDLAQILSDLSAEADRLTLEITDLRLTLLAVQNEAEGEQVALRSLQRRLDDLRI
                     LAGVVAAEGEGISFVIEDPAGQVRQDLLVDTIQELRDAGGEAMAVNGVRLIVSSAFSV
                     RRDRLLLDGQPLTPPYRIVAVGPADTMAKALAIPGGAIDTLEARTEVRTTVEQLAQLT
                     VPARPQTAPFVFGEPVVESRDGE"
     gene            3231..3614
                     /gene="gcvH"
                     /locus_tag="VNU01_12105"
     CDS             3231..3614
                     /gene="gcvH"
                     /locus_tag="VNU01_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWH33403.1"
                     /translation="MNNPDDRRYTEEHEWARAEGGRVTVGITHYAQDALGDVVYVDLP
                     ATGTRVEQGQPFGEVESTKSVSDLYAPVTGVIAERNEALEQHPELVNSDPYGEGWMVV
                     IEVEDTAVVDGLMTAEQYGALTATA"
     gene            3693..4190
                     /locus_tag="VNU01_12110"
     CDS             3693..4190
                     /locus_tag="VNU01_12110"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWH33404.1"
                     /translation="MGETMYCTHCGHSNPAGANFCGNCGRPLAVSDATTGALRLDEGV
                     EEAEAGIEASAVAGELEPGTALLIVVRGPNQGARFLLDRDVVTVGRHPDSDIFLDDIT
                     VSRRHAEFRRQAGAFGVHDTGSLNGTYVNGERADERRLATGDEVQVGKFKLLVVIADG
                     TGGQS"
     gene            4190..>4485
                     /locus_tag="VNU01_12115"
     CDS             4190..>4485
                     /locus_tag="VNU01_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWH33405.1"
                     /translation="MAGDGYTIGEVLNQLKEDFEDITISKIRFLESEGLIFPDRTESG
                     YRKFSADDVDRLRFILTAQRDHYLPLKVIREQLEKLDAGELEPPADPHGNGGAAA"
BASE COUNT          604 a         1580 c         1682 g          619 t
ORIGIN      
        1 cgtcgacgcg cgcctcgcgg aactcgccgc ggcgccgaag gactgcgccg cggcgagttc
       61 cgcgaggcgc gcgtcgacga gggcatgggc ggcggtgcgc tcgtcggcat cgagcgaaga
      121 gaacggggcg cgcccggccg aggccgccag aacgcggcga ttggccaccg acccggcccg
      181 ctcgaccagc atgccgatcc gcgcccagtg gctgatctgc tccgcgaccg accggttctc
      241 ggccggggcg acagccgagg cggtcgccgc gacgtcacct gcgacgcgtg tcggtgtgga
      301 gggtctcttg gtcacgcgcc gactcctgta gcaaactgtg acgatcgtat catgctggca
      361 cgagaacgtc catggcgacg acgcccgcac agcgagacag ccccgcggga agatgcttcc
      421 gcggggctgt ctcgctcgtc gggatgcggg gatttgaacc ccggatctcc ggtcccccag
      481 accggcgccc tggaccaagc tgggccacat cccgtcgctc cggctgggag gccggcgcgc
      541 agagggggag ggtagcgcgg gggtgcgatc ccggccaatg cgcgcgggaa agctaggctt
      601 catcccgtcg gttcggacag gagctcaagg atgcggcggg acttcgactg gggcgcgcgc
      661 aaggaccgcc ccgccgtcgt gcacgacaag gtcctcaccg tcgccaacgg catcagcctg
      721 gcccgcctcg ccggcctgcc cctgttcgtc tggctgatgt tcggcccgca ggcctacggg
      781 tgggcgtggt tcaccctgtt cgtggtcggc tccaccgact tcctcgacgg ctacgtcgcc
      841 cgccgcttcg accaggtcac caagctgggc aagctgctcg acccgctcat cgaccggggc
      901 ctcatggcca ccgtggcgat cagcctcctg gcggccggga tgctgccgtg gttcttcgtg
      961 gcggcgatcc tggggcggga cctgttgatc ctgctgggca cgctggcgtt cctgcgggcg
     1021 gtgccggaga tcgccgtgac cagaaccggc aagttcgcca ccgcctgcct gctgatcggc
     1081 ctgcccggct tcctgctggg ccacatggac tggcccgcgc ggcaggtgtt cctggtcctg
     1141 gcgtggggct acaccaccgt gggcgtggcc ggctactgga tcgccggcct gcagtacgcc
     1201 cgcatcgtgc gcgggctgcg cgggagcggg gcatgagcga gcgcctggcc ggaaggcagc
     1261 gcgaccccgc ggaggccctc ggcctgcacc gcgacgtcgt gtccgactac gagggcctcg
     1321 acaccgcgcg ctggacgcga ccgtggtcgc accggaccgt gctggtgctc accgtcgccg
     1381 gagcgctgct ggccggcttc ctgctcagtg ccgcgctgcg ggccggccgc acggtcgcgc
     1441 tggagcagga cgtgcgccgc accgagctga tcgggctgat cgaggagcgg cgcgcgcaca
     1501 cgcagcggct gtcggcgcag ctggaggcgc tgcgccgcga cgtcgacgcc gccgaggccg
     1561 aggcggccgc cggggtcccg gcgctgcgcg cgcgcctggc ggagctggag gtcgccgccg
     1621 ggctcaccgc gctgcgcgga cccggcctgc gcgtggagct ggccgacgcc tccggcacgt
     1681 gcgcgaccgg gcgcacggag gactgccaga tccaggacac cgacttgcag ctcgcggtga
     1741 acgcgctgtt cgccgtgggc gccgaggcgg tggcggtcaa cggggagcgg gtcatcgcca
     1801 cgacggcgat tcgcagcgcc gggcgctccg tcctggtgaa ctacgaggtg ctcgcctcgc
     1861 cgtacgtgat cgaggcgatc ggcgatcccg aggggctgcg ccgccgcttc gccgagtcgg
     1921 cggtcgcgag cgacttcacg gtgtggaagg acgtgtacgg gttgggcttc tccatccagc
     1981 aggccgacga gctcacgctg ccgccctacg acggcggcct ccggctgcgc gtggccgccc
     2041 ccgcggggga ggcgctgccg tgatcccgct gatcgcgctg ctggtcggcg ccatcctggg
     2101 cctggtcttc cagcccaccg tcccggccgc cctcgcgccc tacctgccgg tcgcggtcgt
     2161 cgcggcgctg gacgccatct tcggggccgt gcgcgcccgc ctggacggcg tcttcagcga
     2221 acgcatcttc ctggtgtcgt ttctgaccaa cgtggtgctg gccgtgttcc tggtgtacct
     2281 gggcgaccag ctcggcgtgg gcagcgacct gtcgaccgcg gtcgtggtgg tcctgggcgt
     2341 gcgcatcttc cagaacctgg cctccatccg ccggcacctg ttccgcgcat gagcgccacg
     2401 tcggcgccgg cgctgcgcgg cccgctgatg cgggtcggcg tcgcgctggc ctgcgggctg
     2461 ctcggcttcc tgctggtggc gcaggtccgc gcgacggaga gcgtcgagga ccgcctcgcc
     2521 ggtgagcgcg aggaggacct cgcgcagatc ctgtcggacc tgtccgcgga ggccgaccgg
     2581 ctcaccttgg agatcaccga cctgcgcctg accctgctgg cggtgcagaa cgaggctgag
     2641 ggcgagcagg tcgcgctgcg cagcctccag cggcggctgg acgacttgcg catcctggcg
     2701 ggcgtcgtcg ccgcggaggg cgagggcatc tccttcgtca tcgaggaccc cgcggggcag
     2761 gtgcgccagg acctgctggt ggacaccatc caggagctgc gcgacgcggg cggcgaggcg
     2821 atggcggtca acggcgtgcg gctgatcgtg tcgtcggcgt tcagcgtgcg gcgcgaccgg
     2881 ctgctgctgg atggccagcc gctgacgccg ccctaccgca tcgtcgccgt ggggcccgcc
     2941 gacacgatgg ccaaggccct ggccatcccc ggcggcgcca tcgacacgct ggaggcccgc
     3001 acggaggtgc gcaccaccgt ggagcagctg gcgcagctca ccgtgccggc gcggccgcag
     3061 acggcaccgt tcgtgttcgg cgagcccgtg gtggagtcgc gcgacggcga atgacgccgg
     3121 gcgcggcggg ccgcgcgcgg ggtagcatcc gcgcgcgaca caccgaccgg cggatgccgg
     3181 tcgcgccggc gccgcacgcc cggtgcaacc aggaggagag tgacgcaggg gtgaacaacc
     3241 cggacgatcg caggtacacc gaggaacacg agtgggcccg ggccgagggc ggccgtgtca
     3301 cggtcgggat cacccactac gcgcaggacg cgctcgggga cgtggtgtac gtggacttgc
     3361 cggccaccgg cacgcgcgtc gagcagggcc agccgttcgg cgaggtggag tccaccaaga
     3421 gcgtctccga tctgtacgcg ccggtcaccg gcgtgatcgc ggagcgcaac gaggcactgg
     3481 agcagcaccc cgagctggtc aacagcgacc cgtacgggga gggctggatg gtggtgatcg
     3541 aggtggagga caccgcggtg gtggacggcc tcatgaccgc cgagcagtac ggagcgctca
     3601 ccgcgaccgc ctgaggcgcc tcgcaggtga accggccgcc ggtgggcggc cctttggtat
     3661 cgttcggagc cgctgcacga cgaccggccg gcgtgggaga gacgatgtac tgcacgcact
     3721 gcggacactc caatcccgct ggtgcgaact tctgcggcaa ctgcggaagg ccgctggccg
     3781 tgtcggacgc gaccacgggc gcactgcgcc tcgacgaggg cgtggaggag gccgaggcgg
     3841 gcatcgaggc ctcggcggtc gccggcgagc tggagcccgg cacggcgctg ctgatcgtgg
     3901 tccggggccc caaccagggc gcgcgcttcc tgctggaccg cgacgtcgtg accgtgggac
     3961 gccacccgga cagcgacatc ttcctggacg acatcaccgt gtcgcgccgc cacgccgagt
     4021 tccgccgcca ggccggcgcc ttcggcgtgc acgacaccgg cagcctcaac ggcacgtacg
     4081 tcaacggtga gcgtgccgac gagcggcgcc tggccaccgg cgacgaggtg caggtcggca
     4141 agttcaagct gctcgtggtg atcgccgacg gcacgggcgg gcagtcctga tggcggggga
     4201 cggctacacg atcggcgagg tcctcaacca gctgaaagag gacttcgagg acatcaccat
     4261 ctccaagatc cgcttcctgg agagcgaggg gctgatcttc ccggaccgca ccgagtccgg
     4321 ctaccgcaag ttcagcgccg acgacgtcga ccgcctgcgc ttcatcctca ccgcccagcg
     4381 cgaccactac ctgccgctca aggtgatccg cgagcagctg gagaagctgg acgccggcga
     4441 gctggagccg cccgccgacc cgcacggcaa cggcggggcg gccgc
//
LOCUS       DATMKC010000413         7050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_321471, whole genome shotgun sequence.
ACCESSION   DATMKC010000413 DATMKC010000000
VERSION     DATMKC010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321471"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VNU01_12120"
     CDS             complement(<1..678)
                     /locus_tag="VNU01_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HWH33406.1"
                     /translation="MSGGPDTDAGGHLTGVPVMLRVDGRLVVGVGAGPVAAAKLLPLL
                     RQGARVRVIAPKADDPIKAAAAEGLLEWERRGYRDGDLEGAVLAVAATDLADVNAAVA
                     AEAQRRALPCVRADGGGSADLMADLRRGPLTLAVSTGGASPALSARVRERLEDDFGPE
                     WGALAALLGELREDPDVRAALAPIPPGERRLKWRSIMDTNILTLLRVGDIQAAREVAF
                     ACLCSSSD"
     gene            complement(675..1406)
                     /locus_tag="VNU01_12125"
     CDS             complement(675..1406)
                     /locus_tag="VNU01_12125"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWH33407.1"
                     /translation="MNNTVGTPVEERSVVTRQIPEASVARLPVYLRALLDLAERGTHT
                     VSSEALADAAGVNSAKIRKDLSYLGSYGTRGVGYDVEYLIHQISRELGLTQDWAVCIV
                     GIGSLGQALAKYGGFAERGFRIAALFDADERMVGQRIGDIPVTDFEEIDRVVKEEEIS
                     IAILAVPGVVAQDVADRVVTAGITSILNFAPRVLQVPDHVSLRKVDLSIELQILSFYE
                     QRKFAEESVRRGTARQRLDEAERGR"
     gene            1554..2765
                     /locus_tag="VNU01_12130"
     CDS             1554..2765
                     /locus_tag="VNU01_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWH33408.1"
                     /translation="MDVEAFVSRERGEIRPTINRSLAFHPAPLPREIALPPETVALLS
                     SATGALYNLGGTGRLLPNPDLLIIPYIRAEAVLSSKIEGTESTISDILKVQVQDESSV
                     PRGDVREVLNYVRAMDHGLARLREGMPLCLRLVKEMQEILLAGVRGQKRRGEFRDEQN
                     WIGPPECRLEDATYVPPPVDYMHEALADWEKFLHLDAARMPILVKVALAHYQFEAIHP
                     FFDGNGRIGRLMIPLTLVAEGALSAPLLYLSVYFERRRTTYYRHLLETSQSGELLPWV
                     EFFLEGVRSQAVDAQARTVRLVEEQRSLRQRLMDSNAPTSVLALAESLLVNPYVTSTQ
                     AMRIVSGTRPTAQKAIDTLAGMGVLVEMTGQRRNRLYFAPSIYEAIYGDFEARDNSRE
                     EGPREATQAAD"
     gene            complement(2762..3019)
                     /locus_tag="VNU01_12135"
     CDS             complement(2762..3019)
                     /locus_tag="VNU01_12135"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWH33409.1"
                     /translation="MPLLGRGPRRVVTVYTRQGCHLCEQAEAVVRRVARRHDIRVVDI
                     DADPALVDRYTVRVPVIEVDGTEIAEFQLDPAALRRALHGD"
     gene            complement(3028..3690)
                     /locus_tag="VNU01_12140"
     CDS             complement(3028..3690)
                     /locus_tag="VNU01_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33410.1"
                     /translation="MTAATSETAAAAVAAVFAARLLGQWRARRRPHALAWTVSLGLFS
                     VASLAAAIGMASGWGPVVFAVYWFTGGLIVVPFLAAGQLMLMDPRRMLIWWTLAGLFA
                     VWALAALALASFDAGALAAADTAGSIPQGKDVFGEGQLAYGLLRFANYTAAVVVVGTA
                     WSAVKTRRLQILLIAVGVIIAGVSFVFIREGLPVGFSASLAVGVAAMYAGFVAAGKPP
                     KR"
     gene            complement(3687..5267)
                     /locus_tag="VNU01_12145"
     CDS             complement(3687..5267)
                     /locus_tag="VNU01_12145"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWH33411.1"
                     /translation="MGGSAGKEREYNVNIAESLRASAARLPDKAAVVFQDSPVTYAEL
                     DQRADRAAAAFQRMGLVPGDRVALMAANAPHFAEALYGAWRAGLVVVPVNVLFTAEEV
                     RHILSDSEARAIVVTEAFAGALRGLREELPGLEEVVVAGGSTAEVGTRRWRDLLDEDA
                     APAAVDTAPDALALLQYTSGTTGQPKGAMLTHANLQANHEQMAQTQLRVEERDILLCV
                     LPLFHIYALNVALAFTLSRGATVLLIERFDALATLQAVERHRASIIIGAPPMYVAWVN
                     TSGVDELDLQCVRFAISGAAPLPRHVLERFQDELGITIWEGYGLSETSPVLTTVAMGE
                     EVKPGSVGRPVPGVELRLLDDKGNEARPGDPGEVVVRGPNVFSGYWRQPEATREVLSE
                     DGWFRTGDVGLLDGGDLYLVDRKKDLIIVSGFNVYPREVEEVLYRHPKIAEAAVIGTS
                     HPYTGEAVKAVVVLRPGEEATADEVTDFCRRFLARFKCPEVVEFAAELPHLPSGKVLR
                     RALRDQPQVQGTGHAEQA"
     gene            5313..6224
                     /locus_tag="VNU01_12150"
     CDS             5313..6224
                     /locus_tag="VNU01_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWH33412.1"
                     /translation="MEREEAREVGGGSRDSGPGHPRLLASTGPLLMTPLGWAMDVIAD
                     AGYQEAELLIAHHSDTRDPDKVLAYAAEAGLRVPVVHGPYMIILRRVLGTDYVEKTRR
                     SLEIAAEIGARTMVAHAPFRWERGARRWLADDADGQAAELGIDFAMENLYPVAGRAFS
                     SAITADDLRAYRHVVFDTSHFAVAGIDLFDAWEALADRVGHLHVSDNFGTGRDSHAPI
                     GSGVLPLEAFLAHVGESGYTGTITLELDCRPYLDSREHLVTFLARERVKAEELLTGAV
                     PAPPRPERPPEQPAERVPDELGSGAGA"
     gene            complement(6221..>7050)
                     /locus_tag="VNU01_12155"
     CDS             complement(6221..>7050)
                     /locus_tag="VNU01_12155"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH33413.1"
                     /translation="GTRSRSTRTTGLPVRAAGPAAARTGSPVVLVERDRVPEATQAEI
                     ERLRPELVSLLGGTSAISPEVEAQIKALGPDVSRLRGDSRFSTATAVSRAHFSAGVPV
                     AYIASGEGFADALSGGAAAAALGGPVLLVQRDAVPAATEEELRRLRPARLVVLGGRQA
                     ISEAVESQLRPHADEVRRVSGPTRYATSAAVSRDAFPGTAETVFVATGAAFADALAGV
                     PAAGAQRAPLLLVEPDRLPAEVAAELDRLRPGQIVILGGERSVSQRLERELRSYQTR"
BASE COUNT         1068 a         2547 c         2476 g          959 t
ORIGIN      
        1 gtccgaggac gagcacagac acgcgaacgc cacttctctc gcagcttgga tgtcgccgac
       61 gcgcagcagg gtcaggatat tcgtgtccat gatggaacgc catttgaggc ggcgttctcc
      121 cggtggaatc ggtgcaagcg cggcgcgcac gtccgggtcc tcgcgcagtt caccgagcag
      181 ggcggccagg gcgccccact ccggcccgaa gtcgtcctcc agccgctccc gcacccgcgc
      241 ggacagggcg ggcgacgcgc cgccggtgga gacggcgagc gtcagcgggc cgcggcgaag
      301 gtccgccatg aggtcggccg agccgccgcc gtcggcgcgc acgcaaggca gcgcacggcg
      361 ctgcgcctcc gccgccacgg cggcgttcac gtcggccagg tcggtcgccg ccaccgccag
      421 gaccgcgccc tccaggtcgc cgtcgcggta gccgcggcgc tcccactcca gcaatccctc
      481 cgcggcggcg gccttgatcg ggtcgtcggc cttgggggcg atcacgcgca cgcgggcgcc
      541 ctggcgcagc agcggcagca gcttcgcggc ggcgacgggg ccggcgccga cgcccacgac
      601 cagccggccg tcgacccgga gcatgacggg cacgccggtc aggtgtccgc cggcgtccgt
      661 gtccggaccg ccgctcaccg gccgcgctcg gcctcgtcca ggcgctggcg cgcggtcccg
      721 cggcgcaccg actcctccgc gaacttgcgc tgctcgtaga aggacaggat ctgcagctcg
      781 atggacaggt cgaccttgcg caggctgacg tggtcgggga cctgcaggac gcgcggggcg
      841 aagttgagga tggaggtgat gcccgcggtg accacgcgat cggcgacatc ctgggccacc
      901 acgcccggca cggccaggat ggcgatggag atctcctcct ccttgaccac ccggtcgatc
      961 tcctcgaagt cggtcacggg gatgtcaccg atgcgctggc cgaccatgcg ctcgtccgcg
     1021 tcgaacagcg cggcgatgcg gaagccgcgc tcggcgaagc cgccgtactt ggccagcgcc
     1081 tggcccaggg agccgatgcc gacgatgcac accgcccagt cctgcgtgag gccgagctcc
     1141 cggctgatct ggtggatcag gtactcgacg tcgtagccga cgccgcgcgt cccgtaggag
     1201 ccgaggtagg acaggtcctt gcggatcttg gcggagttca ccccggccgc gtccgcgagc
     1261 gcctcgctcg agacggtgtg agtgccgcgt tcggccaggt cgagcaaggc ccgcaggtac
     1321 acgggcagcc gcgcgacgct cgcctcggga atctggcgtg tcacgacgga gcgctcctcg
     1381 acaggggtgc caacggtgtt gttcacaagg gtacttgcag agtcgtgagt ggcaagccgg
     1441 gatgccccga cgaacgaaag tcgactttcg catgtggaca gtcaagtaaa gctgtgggct
     1501 gataacttta cttccagtcc cgtccgcgca agtcgacttg ctcagggaat cacatggatg
     1561 tcgaggcctt cgtgagccga gagcgagggg agatccggcc gacgatcaac cgttcgttgg
     1621 cattccaccc tgcgccgctg ccgcgagaaa ttgcactgcc cccggagaca gttgccctcc
     1681 tcagttccgc cacgggcgcg ctctacaacc tcggcggcac gggtcgactg ctgccgaatc
     1741 ccgatctgct catcatcccg tacatccgcg ccgaggctgt cctcagttcc aagatcgaag
     1801 gcaccgagtc gacgatctcc gacatcctca aggtccaagt tcaggatgag tcgagtgtcc
     1861 cgcgcggaga cgtgcgagag gtactcaact atgtgagagc gatggatcat ggcctcgcaa
     1921 ggctgcgcga gggcatgccc ttgtgcctga ggctggtcaa ggagatgcag gagatcctcc
     1981 ttgccggcgt tcggggtcag aagcgtcgag gcgagttccg ggacgaacaa aactggatcg
     2041 ggccgcccga gtgccgcctg gaagatgcaa cctacgtgcc gccacctgtg gactacatgc
     2101 acgaagcgct cgccgattgg gagaagttcc tccatctgga cgctgcacgc atgcccatcc
     2161 tggtcaaggt ggcgctcgcc cactatcagt tcgaagcgat ccacccgttc tttgacggga
     2221 acgggcggat tgggcggctg atgataccgc tcacgctggt cgccgaaggc gccttgtccg
     2281 ctccactgct gtacctgtct gtgtacttcg agcgacggcg caccacctac taccgacacc
     2341 tcctcgagac cagccaatcc ggtgaactgc tcccgtgggt cgagttcttc ctggaaggcg
     2401 tgcgttccca ggccgtggac gcccaggccc gaactgtgcg gctcgttgag gagcaacgct
     2461 cacttcgcca acgtctcatg gattcgaacg cacccacgag cgtgcttgcc ttggccgagt
     2521 cgctgctcgt caatccctac gtcacctcga cacaagccat gaggatcgtg agcggaacgc
     2581 gacctactgc gcagaaggcc atcgacacgc tcgccgggat gggtgtattg gtggagatga
     2641 cggggcagcg caggaaccgg ctgtacttcg caccgtcgat ctacgaagcg atctacggcg
     2701 acttcgaggc ccgcgacaat agccgagagg aaggccctcg tgaggcgacg caggccgccg
     2761 actagtctcc gtgcagcgcg cggcggagcg cggcggggtc caactggaac tcggcgatct
     2821 cggtgccgtc cacctcgatg acggggacgc ggaccgtgta gcggtccacc agggccgggt
     2881 ccgcgtcgat gtccacgacg cggatgtcgt ggcggcgggc cacgcggcgc acgaccgcct
     2941 ccgcctgctc gcacaggtgg cagccctggc gcgtgtagac ggtgacgacg cgccgggggc
     3001 cgcggccgag gagtggcacc gggccgctca gcgcttcggc ggcttgcccg ccgccacgaa
     3061 gcccgcgtac atggcggcca cgccgacggc cagcgacgcc gagaagccca ccggcaagcc
     3121 ctcgcggatg aacacgaacg acaccccggc gatgatcaca cccacggcga tcagcaggat
     3181 ctgcaagcgg cgcgtcttga ccgccgacca tgccgtgccg accaccacga ccgcggcggt
     3241 gtagttggcg aagcgcagca gcccgtaggc gagctggccc tcaccgaaga cgtccttgcc
     3301 ctgcgggatc gacccggcgg tgtccgccgc cgcgagcgcg ccggcgtcga agctcgccag
     3361 cgccagcgcc gccagcgccc acacggcgaa caggcccgcc agcgtccacc agatcagcat
     3421 gcggcgcggg tccatgagca tgagctggcc cgccgccagg aacggcacca cgatgagccc
     3481 gccggtgaac cagtacaccg cgaacaccac cggtccccag ccggaggcca tgccgatcgc
     3541 ggcggccagc gaggccacgg agaacaagcc cagcgacacc gtccacgcca gggcgtgcgg
     3601 gcgccggcgc gcgcgccact ggccgagcag gcgcgcggcg aacaccgcgg ccaccgcggc
     3661 ggccgccgtc tcgctggtgg ccgcggtcac gcctgctccg cgtgccccgt gccctgaacc
     3721 tggggctggt cgcgcagcgc gcgccgcagc accttgccgc tgggcaggtg cggcagctcc
     3781 gcggcgaact ccaccacttc cgggcacttg aagcgcgcga ggaagcggcg gcagaaatcg
     3841 gtgacttcgt cggcggtggc ctcctcgccg ggacgaagga cgaccacggc cttcaccgcc
     3901 tcgccggtgt aggggtgcga ggtgccgatg accgcggcct cggcgatctt cgggtgccgg
     3961 tacaggacct cttccacctc gcgcgggtac acgttgaagc cgctgacgat gatgaggtcc
     4021 ttcttgcggt ccaccaggta caggtccccg ccgtcgagca ggcctacgtc gccggtgcgg
     4081 aaccagccgt cttcgctgag cacctcgcgc gtcgcctcgg gctggcgcca gtagccggag
     4141 aacacgttcg gcccgcgcac caccacctcg ccgggatcgc cgggccgcgc ctcattgccc
     4201 ttgtcgtcga gcagccgcag ctccacgccg ggcaccggcc gcccgacgct gccgggcttg
     4261 acctcctcgc ccatggccac ggtcgtgagc acgggcgagg tctccgacag cccgtagccc
     4321 tcccagatcg tgatgccgag ctcgtcctgg aagcgttcca gcacgtggcg cggcaacggc
     4381 gccgcgccgg agatggcgaa gcgcacgcac tgcaggtcca gctcgtccac gccggaggtg
     4441 ttcacccagg ccacgtacat cggcggcgcg ccgatgatga tcgaggcgcg gtggcgctcc
     4501 accgcctgca gggtcgccag cgcgtcgaag cgctcgatca gcagcacggt ggcgccgcgc
     4561 gacagcgtga acgccagcgc cacgttgagc gcgtagatgt ggaacagcgg caggacgcac
     4621 agcaggatgt cgcgctcctc cacgcgcagt tgcgtctggg ccatctgctc gtggttggcc
     4681 tgcaggttgg cgtgcgtgag catcgcgccc ttgggctggc cggtggtgcc cgaggtgtac
     4741 tggagcaggg ccagcgcgtc gggcgccgtg tccacggcag cgggggcggc gtcctcgtcc
     4801 agcaggtcgc gccaccgccg cgtgccgacc tccgcggtgg acccgccggc gacgacgacc
     4861 tcctccagcc ccggcagctc ctcgcgcagg ccgcgcaacg cgccggcgaa cgcctccgtg
     4921 accacgatgg cccgcgcttc gctgtcgctg aggatgtggc ggacctcctc cgccgtgaac
     4981 agcacgttga ccgggacgac caccaggccg gcccgccagg ccccgtacag cgcctcggcg
     5041 aagtgcggcg cgttggccgc catgagcgcg acgcggtcgc cgggcaccag gcccatgcgc
     5101 tggaacgcgg cggcggcgcg atcggcccgc tggtcgagct cggcgtaggt gaccggggag
     5161 tcctggaaga cgaccgcggc cttgtccggc agccgggcgg cactggcccg cagcgattcg
     5221 gcgatgttca cgttgtattc ccgctccttg ccggcgcttc ctcccacgca ccggcagcgt
     5281 agcgcagcgc gcacccgggg tagcctggcg ccatggagcg cgaggaggcc cgcgaggtgg
     5341 gcggcggcag ccgggacagc ggtcccggac acccccgtct cctcgcttcg acgggtccgc
     5401 tgctgatgac cccgctgggc tgggcgatgg acgtgatcgc cgacgcgggc tatcaggaag
     5461 cggagctgct gatcgcccac cacagcgaca cgcgcgaccc ggacaaggtg ctggcgtacg
     5521 cggccgaggc cggcctgcgc gtgcccgtgg tgcacgggcc gtacatgatc atcctgcggc
     5581 gcgtactggg caccgactac gtcgagaaaa cgcgccggtc actggagatc gccgccgaga
     5641 tcggcgcacg caccatggtg gcgcacgcgc cgttccgctg ggagcgcggc gcccggcggt
     5701 ggctggcaga tgacgccgac ggccaggcgg cggaactggg catcgacttc gcgatggaga
     5761 acctgtaccc ggtggccggc cgggcgttct cctcggcgat cacggccgac gacctgcgcg
     5821 cgtaccgcca tgtcgtattc gacaccagcc actttgcggt cgcgggcatc gacctgttcg
     5881 acgcctggga ggcgctggcg gaccgcgtcg gtcacctgca cgtgtccgac aacttcggca
     5941 cgggccgcga cagccacgcc ccgattggca gcggcgtgct gccgctggag gccttcctgg
     6001 cccacgtcgg cgagtccggc tacaccggca cgatcacgct ggagctggac tgccgcccct
     6061 atctggacag ccgcgagcac ctggtcacgt tcctggcccg cgagcgcgtg aaggccgagg
     6121 agctgctgac cggcgcggtg cccgcgccgc cccgcccgga gcggccgccg gaacagcccg
     6181 cggagcgcgt gccggacgag ctgggatcgg gcgccggcgc ctagcgcgtc tggtaggagc
     6241 gcagttcccg ctccaggcgc tgcgacacgg accgttcacc gcccaggatg acgatctgcc
     6301 ccgggcgcag gcggtccagc tcggcggcca cctccgcggg caggcggtcc ggctccacga
     6361 gcagcagcgg ggcgcgctgc gcgcccgccg ccggcacgcc cgccagcgca tccgcgaacg
     6421 ccgcgcccgt cgccacgaac accgtctccg cggtgccggg gaacgcgtcc ctgctcacgg
     6481 cggcggaggt ggcatagcgc gtgggcccgc tgacacggcg cacctcgtcg gcgtggggac
     6541 ggagttggga ctccacggct tccgagatgg cctggcggcc accgagcacg accagccgcg
     6601 ccggccggag gcgccgcaac tcttcttccg tcgccgccgg gaccgcgtcg cgctgcacca
     6661 gcagcaccgg accacccagt gcggccgccg ccgcgccgcc ggacagcgcg tcggcgaaac
     6721 cctcgccgct ggcgatgtag gcgacgggca cgcccgcgct gaaatgggcg cgggagaccg
     6781 ccgtcgcggt cgagaagcgg ctgtcaccgc gcaggcggga cacgtccggt cccagcgcct
     6841 tgatctgcgc ctccacctcg gggctgatgg ccgacgtgcc gccgagcagg ctcaccagct
     6901 ccggccgcag ccgctcgatc tcggcttggg tggcctcggg gacacggtcg cgctccacca
     6961 ggacgacggg gctgccggtg cgcgccgcgg ccggcccggc ggcgcgcacc ggcagccccg
     7021 tcgtcctggt ggagcgcgac cgtgtccccg
//
LOCUS       DATMKC010000414         5718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_9207, whole genome shotgun sequence.
ACCESSION   DATMKC010000414 DATMKC010000000
VERSION     DATMKC010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9207"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VNU01_12160"
     CDS             <1..1048
                     /locus_tag="VNU01_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33414.1"
                     /translation="DCADRSDVGVPFPCTVTASVANAGPHPDAVADVLTRLELPPDCA
                     ASSPGAVQDDLAVSATQFTTVVSVWTVACGQRSFHPITAHVDATLDQLHVQDPDETND
                     SGSAATVVEIFEAADLAVLDMDIACDEYADPRRFSCVTTTTVTNHGVADGVATRTVVD
                     VQAPEGCAVTGSPATRLDDLDAGETADYVDTFAIACHDAARHVVHARALIRADEPHAE
                     DRDATNDRGAVDWLPIDIKPGSFPNAVNVKQKGLIPVAVLSTPTFDAPAQVVASSLRF
                     GATGIEESWVRCGAPEDVDGDGLADLVCHFDATLTGFDCSSTVGVLTGRLLDGTAFES
                     QYTIKVTPCRGDAR"
     gene            1457..1894
                     /locus_tag="VNU01_12165"
     CDS             1457..1894
                     /locus_tag="VNU01_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33415.1"
                     /translation="MMDDDDSWWTDPLPERLLTLLERDLLAVDIDTFWDRKDALERML
                     PERDSTPEQLRAARERAAAVVPADLLAAFIEAIDAPDGPAFTLDERLGDWESLRVDHE
                     KLVGHLLLVHDEPVPTLRAMSYLDLEEHHRLLHERAVQGRAVQ"
     gene            2237..2668
                     /locus_tag="VNU01_12170"
     CDS             2237..2668
                     /locus_tag="VNU01_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33416.1"
                     /translation="MIDDDILEPLPPDLVAVLERDLLAVDIDTFWDRKDALTRIVPER
                     GPVNDEHRAARVRAAAALPAELFEAVVDFLDTPGAPLSTLDEVLSDWESVRVDPEKLV
                     GHALLIHQEPPESILTMTYLDLEEYHRVLHERAAARRPPGR"
     gene            2711..3544
                     /locus_tag="VNU01_12175"
     CDS             2711..3544
                     /locus_tag="VNU01_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33417.1"
                     /translation="MPTSVATRLRDLWGARELLRNLVAKELKVRYKQSALGFAWSLLT
                     PLLMTGIFTVVFATFLRIPVADFATFFLAGYLVWQFFANSVNSSVGAIVGNGPLIRKV
                     WFPREVLPLSLVLAQGVHFLLALAATTPLFLWQRGNFLPYLPALLVGMVLLVLFTAGV
                     SMIFAAADVGFRDLQELTQVIFLAWFYATPVIYPLVFVQQPAAAWALPIVRANPMTWF
                     VELFQTPLYGVVVNMQGASLPPGWPHPQTLAVCAIFAVGTFLVGYALFHRWAVNFAKE
                     V"
     gene            4104..5429
                     /locus_tag="VNU01_12180"
     CDS             4104..5429
                     /locus_tag="VNU01_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH33418.1"
                     /translation="MSAHEQDRLAIVVQDVWETFRIYHERPGGLKERLAKLRRAHYTE
                     FHALKGVSLKIRHGESVGLIGHNGSGKSTLLKVIAKILPPDQGSVATSGRLSTLLELG
                     AGFHQDLSGRENVYMNGAILGLSRAEVDAAFDSIVDFAGVREFIDTPVRNYSSGMYVR
                     LGFAVAVHVDPDILLVDEVLSVGDAAFQEKSLARMRGFNQRGKTVVMVSHDLNAVQAL
                     CERTVVLDHGEIVFDGPTEEAVEIHRELMSGGERPDTGAEEQPGRTGTGQVRVREARL
                     EHDGQAAGGAVPTGTKARLTLDIEARGDVTAGGGLSVGVNVRRPEVAPAVYETRTAWR
                     GLYVAPPPPGASLSVAFDLDMALLSGHYVVDLLIGNAETNVIHDRWLEAVAFDVEAPD
                     YEYGVAPLGAAITVHNPDGVWPADSVPPPLEEGGPRHHPNRDNPSAGEA"
     gene            5430..>5718
                     /locus_tag="VNU01_12185"
     CDS             5430..>5718
                     /locus_tag="VNU01_12185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH33419.1"
                     /translation="MRVVACVVNWNTAAELPGVLASLRALEGLTEIVVVDNASHDGSA
                     DVVRAHPDVRLVTNDRNRGFAGGANQGIGMAVRIGADAFLVCNPDVRLDPAY"
BASE COUNT          798 a         2004 c         2065 g          851 t
ORIGIN      
        1 ggactgcgcg gaccgcagcg acgtcggcgt ccccttcccg tgcacggtca cggccagtgt
       61 ggccaacgcc ggcccgcatc ccgacgccgt ggcggacgtc ctcacgcggc tcgagctgcc
      121 gcctgactgt gcggccagca gccccggcgc cgtccaggac gacctggccg tgagcgccac
      181 ccagttcacg acggtggtca gcgtgtggac ggtggcctgc ggccagcgca gcttccaccc
      241 gatcaccgcc catgtggacg cgacactgga tcagttgcac gtccaggacc cggacgagac
      301 caacgacagc ggctcggccg ctacggtggt cgaaatcttc gaggcggccg acctggccgt
      361 gctcgacatg gacatcgcct gcgacgagta cgccgatccc cggcggttca gctgcgtcac
      421 cacgaccacc gtgacgaacc acggcgtggc cgacggcgtc gccacccgca cggtcgtgga
      481 cgtgcaggcg ccggagggct gcgctgtcac cggcagcccc gcgacgcgtc tggacgatct
      541 cgacgccggg gagacggccg actacgtgga cacgttcgcc atcgcctgcc atgacgcggc
      601 acggcatgtg gtgcatgcgc gcgctctgat ccgggccgat gagccgcacg ccgaggaccg
      661 ggacgcgacc aacgaccgcg gcgccgtcga ctggctgccc atcgacatca agccgggcag
      721 cttcccgaat gcggtcaacg tgaagcagaa gggcctgatc ccggtcgcgg tgctgtcgac
      781 gccgacgttc gacgcgcctg ctcaggtggt ggcgtcgagc ctgcgcttcg gcgcgaccgg
      841 aattgaggag tcctgggtgc gttgcggcgc accggaggac gtcgacggcg acgggctggc
      901 cgacctggtg tgccacttcg acgcgacgct gaccggcttc gactgctcca gcacggtggg
      961 tgtgctcact ggccggctgc tggacgggac cgccttcgag agccagtaca cgatcaaggt
     1021 gacgccgtgc cgtggtgacg cacgatgaac atcgcgctgc gggtatcgcc gcaccgtcga
     1081 gatcgcgagt gaccatcggc cagtgcctcc gtcacgctct gagatccgtg gacggagaag
     1141 gcggaaacat ccgttatttt ggtcgtgaca gaacggttcg ggtgcgtcat gatccgggcg
     1201 tccattgggg ggcgtgcgaa cctcgagttc cgggggagcc gacgcgccgc tcgcggtcag
     1261 caccgcgagc ggcgacgcgt gggggcgcgg cgaaggggtc gttcgcgggg agcgcggcgg
     1321 cccctttccg tgcggccgcg accgcaatgc tcgccgtgtg gatgtctgat cgaccgacgg
     1381 gattgccagc ggtgtggcgg gcgggcggca aaaaaagatt ggcgttgccg ggtgggctcg
     1441 tgcgagcgtg ccggtcatga tggatgacga cgactcctgg tggaccgacc cgctgcccga
     1501 gcggctgctg acgctgctgg agcgggacct gctggcggtg gacatcgaca cgttctggga
     1561 ccgcaaggac gccctggagc ggatgctgcc ggagcgcgac tcgacgcccg agcagttgcg
     1621 cgcggcgcgc gagcgggccg cggcggtggt gcccgccgac ctgctggcgg cgttcatcga
     1681 ggcgatcgac gcgcccgacg ggccggcgtt cacgctggat gagcggctgg gggactggga
     1741 gagcctgcgg gtggaccatg agaagctggt cggccacctg ctgctggtcc acgacgagcc
     1801 cgtgccgacc ctgcgggcga tgtcctacct ggatctggag gaacaccacc gcctgctgca
     1861 cgagcgcgcc gtccaaggcc gcgccgtcca gtagcggcgg atcgccacca ggcggttggc
     1921 gatgaccgtc gagtcgggcc acccgaaaca tccgttattt tggtcgtgac agaacggcct
     1981 gggtgcgtca cgatcccgtc gtccatccgg ggggcgccag ggaagttccg ggagccgatg
     2041 cgccgctcgc ggtcagcacg gcgagccggc gacaggtggg ggcgcggcaa aggggtcgtt
     2101 cgcggggagc gcggcggccc cctttccgtg caccgcgacc gcctgcccgc cggagccggc
     2161 gcgggccgac gagttaccca ccggcccggc gaatttcctc tggcgcttcc cgccgtctcg
     2221 tgcaaaggtg cccgccgtga tcgacgacga catcctggag cccctgccgc cggacctcgt
     2281 ggcggtgctc gaacgcgacc tgctcgcggt ggacatcgac acgttctggg accgcaagga
     2341 cgcgctcacg cgcatcgtcc cggagcgcgg ccccgtcaac gacgagcatc gcgccgcgcg
     2401 cgtgagggcg gccgccgcgc tgcccgcgga gctgttcgag gcggtcgtgg acttcctcga
     2461 cacaccgggc gcgccgctgt cgacgctcga cgaggtgctg agcgactggg agagcgtccg
     2521 cgtcgacccg gagaagctcg tggggcacgc cctgctgatc caccaggagc cgcccgagtc
     2581 catcctgacg atgacctacc tggacctgga ggagtaccac cgcgtgctgc acgagcgcgc
     2641 cgccgcccgc cgcccgcccg gccggtagcg cgcaccgggc cgcgcggccc ccgctgctac
     2701 gatcgcgcgc atgccgacct ccgtggccac ccggcttcgc gacctgtggg gcgcccggga
     2761 gctcctgcgc aacctggtcg ccaaggagct gaaggtccgc tacaagcagt cggccctggg
     2821 cttcgcctgg tcgctgctca ccccgctgct catgacgggc atcttcaccg tggtgttcgc
     2881 gaccttcctg cgcatcccgg tggccgactt cgcgacgttc ttcctggccg gctacctggt
     2941 gtggcagttc ttcgccaaca gcgtgaacag ctccgtgggc gcgatcgtcg gcaacggccc
     3001 gctcatccgc aaggtgtggt tcccgcgcga ggtgctgccg ctgtcgctgg tgctggccca
     3061 gggcgtccac ttcctgctgg cgctggccgc caccacgccg ctgttcctgt ggcagcgcgg
     3121 caacttcctg ccgtacctgc cggccctgct ggtcggcatg gtgctgctgg tgctgttcac
     3181 cgccggcgtg tcgatgatct tcgcggccgc cgacgtgggt ttccgcgacc tgcaggagct
     3241 cacgcaggtc atcttcctgg cgtggttcta cgcgaccccg gtgatctacc cgctggtgtt
     3301 cgtgcagcag ccggccgccg cgtgggcgct gcccatcgtg cgggccaacc cgatgacgtg
     3361 gttcgtggaa ctgttccaga cgccgctgta cggggtggtg gtgaacatgc agggcgcgag
     3421 cctgccgccg ggctggccgc acccccagac gctggccgtc tgcgccatct tcgccgtggg
     3481 gacgttcctg gtcggctacg cgctgttcca ccgctgggcc gtgaacttcg ccaaagaggt
     3541 ctgacggtcg ccagaccgac cgccgggcgc gcgctccagc gggccaacac cgtatatccg
     3601 gtattttggt cgtgacaggc ggcggcatct gtcagaagat gacgaccgtc ctccgcgaag
     3661 gctggggggc aggatgcacg ctccgcggcg tgcggcggtc cacctggggc ggtggcacgt
     3721 ttcccgcgga ggcgtgggac ctgacgcctt cgggcaggac gggtgagcgg ccgttcgcgc
     3781 cacgcgcgtc ggccgctcat cgcgtccgga cacacacccc ggtcccgccg gtttgtcatg
     3841 cgccatcgga ttcggtcatg acaaaccatc ccgattcgcg catcattcga ggtgctctct
     3901 cggcatccgg ggggcagtgg aaccgcgtcg gcgtgtggtg agccccaggg cgcatcgcgt
     3961 cgagtcgccg gcatgtgtga tcgctgagtg gattcttgaa ggagcacggt gagcggccgt
     4021 cagcgcgcga gcgcggcggc cgttcactgc gtccggggcc ggccaggagg gctttcgccg
     4081 cccggcgccg atagactccg ccggtgtctg cgcacgaaca ggaccgcctc gccatcgtcg
     4141 tccaggacgt gtgggagacg ttccgcatct accacgagcg ccccggcggc ctgaaggagc
     4201 gcctggccaa gctgcggcgc gcgcactaca ccgagttcca cgccctcaag ggcgtgagcc
     4261 tgaagatccg ccacggcgag agcgtcggcc tgatcgggca caacggctcc ggcaagtcca
     4321 cgctgctcaa ggtgatcgcc aagatcctgc cgcccgacca gggcagcgtc gccaccagcg
     4381 gccgcctgtc gacgctgctg gagctgggcg ccggtttcca ccaggacctg tccgggcgcg
     4441 agaacgtgta catgaacggc gcgatcctgg gcctgtcgcg cgcggaggtc gacgccgcgt
     4501 tcgactccat cgtcgacttc gccggcgtgc gcgagttcat cgacacgccg gtgcgcaact
     4561 actcctccgg catgtacgtg cggctgggct tcgccgtcgc cgtgcacgtc gaccccgaca
     4621 tcctgctggt cgacgaggtg ctgtcggtgg gcgacgcggc gttccaggag aagtccctgg
     4681 cgcgcatgcg cggcttcaac cagcgcggca agaccgtggt gatggtgtcc cacgacctca
     4741 acgccgtgca ggcgctgtgc gagcgcaccg tggtgctcga ccacggcgag atcgtcttcg
     4801 acggccccac cgaggaggcc gtcgagatcc accgcgagct catgtccggc ggcgagcgcc
     4861 cggacaccgg cgccgaggag cagcccggcc gcaccgggac cgggcaggtc cgcgtgcgcg
     4921 aggcgcgcct ggagcacgac ggccaggccg ccgggggcgc cgtgccgacc ggcaccaagg
     4981 cgcgcctgac cctggacatc gaggcgcgcg gcgacgtcac cgccggcggc gggctgtcgg
     5041 tcggcgtcaa cgtgcgccgg ccggaggtcg cccccgcggt gtacgagacg cgcacggcgt
     5101 ggcgcgggct gtacgtggcg ccgccgccgc cgggcgcgtc gctcagcgtg gcgttcgacc
     5161 tcgacatggc gctgctgtcc ggccactacg tcgtggatct gctgatcggc aacgccgaga
     5221 ccaacgtgat ccacgaccgc tggctggagg cggtggcctt cgacgtggag gcgccggact
     5281 acgagtacgg ggtggcgccg ctgggcgcgg cgatcaccgt gcacaacccc gacggcgtgt
     5341 ggccggcgga cagcgtgccg ccgccgctgg aggagggcgg cccccgccac caccccaacc
     5401 gcgacaaccc gtccgccggc gaggcgtaag tgcgggtcgt cgcctgtgtc gtcaactgga
     5461 acacggcggc ggagctgccg ggcgtgctgg cgtccctacg cgcgctggag ggcctcacgg
     5521 agatcgtcgt ggtcgacaac gccagccacg acggcagcgc cgacgtcgtc cgcgctcatc
     5581 cggacgtgcg gctggtcacc aacgatcgca accgcggttt cgccggcggc gccaaccagg
     5641 gcatcggcat ggcggtgcgc atcggcgccg acgcgttcct ggtctgcaac cccgacgtgc
     5701 gcctggaccc cgcctacc
//
LOCUS       DATMKC010000415         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_9245, whole genome shotgun sequence.
ACCESSION   DATMKC010000415 DATMKC010000000
VERSION     DATMKC010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9245"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VNU01_12190"
     CDS             complement(<1..245)
                     /locus_tag="VNU01_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33420.1"
                     /translation="MNWLAYALATVVLWTAWSFLGVIALKEVSAAHATLVFGVAVVVV
                     GVASLALGNRGGPWSPSGLGVAAISGGCGALGMATFY"
     gene            complement(337..810)
                     /locus_tag="VNU01_12195"
     CDS             complement(337..810)
                     /locus_tag="VNU01_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33421.1"
                     /translation="MAASARVRVYKIKSRPGIRYACDLRRRRTRFLTEEVLGSSSKSA
                     TSFRVAGAYVAYKSEECDRGEECTTSIFVVNGVTGRYRPQPGPVAGVASALRVNADGV
                     AAYIDTRDETIAVRAVTRSSALVLAESTEIDPSSLALGNGWIYWTEADRPRSASI"
     gene            complement(1068..1934)
                     /locus_tag="VNU01_12200"
     CDS             complement(1068..1934)
                     /locus_tag="VNU01_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH33422.1"
                     /translation="MERITPPSPLLEPDEILLEPLEQLLREAGRFRLLEPHEEIELAQ
                     RIERGDLAAKDRLINCNLRLVVSVARKYQGQGLSMNDLVQEGMLGLIRAAEKFDWRKG
                     FRFSTYATIWIRQALQRGLDNTGRTVRLPAHVAQRARKVERAERELTVKLGEEPTLDQ
                     IAEETGLTPEEVDLLRSHARTLASLDAGVGEDGDTPLGALIAISGESTEEKVERTIEQ
                     RTVRDALEALPADERNVVNLRFGTGDEGEHSIEQTARRLGVPVREARAAEQRALAKLA
                     DLAELAALRTAA"
BASE COUNT          415 a          894 c          902 g          398 t
ORIGIN      
        1 aagtagaacg tcgccatccc gagcgctccg caacccccgc tgatcgcagc gacgccgaga
       61 cctgagggcg accagggccc gccgcggttg ccgagggcaa ggcttgccac accgaccacc
      121 accacggcaa ccccgaacac gagcgtggcg tgggcggcac tgacctcctt gagtgcgatg
      181 acgccgagga agctccacgc cgtccacagg acgacggtgg ccaacgcgta ggcgagccaa
      241 ttcacgacgg tcaagctagc tgggccgcgc gacgctgtcg cgttcgccgc gagcggcagc
      301 gccggcgaat accgcttccg gctcggtgcc aaagcgtcag atcgacgccg agcgaggccg
      361 gtcggcctcc gtccagtaga tccacccgtt accaagcgcg agggaactcg ggtcgatctc
      421 ggtgctctcg gcgagcacca gagcactcga ccgggtgact gcgcgaaccg cgatggtctc
      481 gtcgcgggtg tcgatgtagg cggcgactcc atcggcgttg acgcgcaggg cagaagccac
      541 gcctgcaacg gggccgggct gggggcgata gcgcccggtg acgccgttga cgacgaagat
      601 gcttgttgtg cactcctcgc cccgatcgca ttcctcgctc ttgtacgcga cgtatgcgcc
      661 cgccactcga aaggaggttg cactcttgct gctggagccc aacacctctt cggtcaagaa
      721 ccgcgtccgg cgtcgccgta gatcgcaggc gtagcggatg cctgggcggc ttttgatctt
      781 gtagacgcga actcgtgcgc tcgcggccac ggtcgatccg gccgtcgcgc atgacctagg
      841 ccctgtgtct gccggagcgg cggacggcgg gatcatcagc gacaagccaa cgccgaggat
      901 gcagagagga cgccgcatgt cttcccgaac gccgcgcgtc cacagaagtt gcaggattgc
      961 ccgaccgtcg ccgcctgccc gcgcgacggc tccagaagca atcctgccgt gcagcgcagg
     1021 ccccacaaac gacgaccgcc ccgggatccg gggcggtcgg ggcggcccta ggccgcggtg
     1081 cgcagggcgg cgagctcggc caggtcggcc agcttcgcca gggcgcgctg ctcggcagcg
     1141 cgggcctcgc ggacgggaac cccgaggcgg cgggcggtct gctcgatcga gtgctcgccc
     1201 tcgtcgcccg tgccgaagcg caggttgacg acgttgcgct cgtcggccgg cagggcctcc
     1261 agcgcgtcgc ggacggtgcg ctgctcgatc gtgcgctcga ccttctcctc ggtgctctca
     1321 ccggagatgg cgatcagggc gccgagcggc gtgtcgccgt cctcgcccac gccggcgtcg
     1381 aggctggcga gggtgcgggc gtgcgagcgc aggaggtcga cctcctcggg cgtgaggccc
     1441 gtctcctcgg cgatctggtc gagcgtcggc tcctcgccga gcttgacggt cagctcgcgc
     1501 tcggcgcgct cgaccttgcg ggcgcgctgg gcgacgtgcg ccggcaggcg gacggtacgg
     1561 cccgtgttgt cgagaccgcg ctgcagcgcc tggcggatcc agatcgtcgc gtacgtcgag
     1621 aaacggaagc ccttgcgcca gtcgaacttc tcggccgcac ggatgaggcc gagcatgccc
     1681 tcctggacga ggtcgttcat cgacagtccc tggccctggt acttccgggc cacggacacg
     1741 acgagacgga ggttgcagtt gatgagccgg tccttcgccg cgaggtctcc gcgctcgatc
     1801 cgctgcgcca gctcgatctc ctcgtggggc tcgagcaggc ggaagcggcc ggcttcgcgc
     1861 aggagctgct cgaggggctc cagcaggatc tcgtcgggct caagcagcgg ggacggggga
     1921 gtgatgcgct ccaagtaggt tccttgttgt ggggggatat ccaactactg gagtcggatt
     1981 ttacggcaaa acggttgcca gcgcaactat gcgccgggac gatgacgggg tcgctacctt
     2041 cccggtacgt cctaaaacgc gtctaagacc actctggtgc gatgtctgac gaccgtgagg
     2101 acgccatatc gcgcgcgagc gccttcgccg cgtcgaaacc acatacggag cccggcgtgc
     2161 cgtggctcca gcaggtgctc accgaggatt cgatgggacg cgccgtcgtt cctcgtcgtg
     2221 aagtcgtaca ccgtaccgaa gaaagggcaa caccgccacc cggccacccc gccaacctgg
     2281 cgcggccggg ctcgaacccg ggtgcggccg gcgcgcatcg ctcgcgaggc atgcgaccac
     2341 cgtcgcaccg cggagcgccg cgcgccagca gcatcgcttc gatcagggat cagatgcgct
     2401 gattccgggc agtgccacat ccgccacggc cttctctgcg taaggagggg tgcgccggtt
     2461 ggcgacgaac ctgaatcctg tacccctact tggaggtccg caatgccgga aacgagggac
     2521 aacacccccg cccgcaccta cgcgctggtc atcggcgccg tcctgacgct cgtcggaatc
     2581 gtcgggttcc tggtcgaagc cagcttcga
//
LOCUS       DATMKC010000416         8488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_58094, whole genome shotgun sequence.
ACCESSION   DATMKC010000416 DATMKC010000000
VERSION     DATMKC010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58094"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VNU01_12205"
     CDS             complement(<1..400)
                     /locus_tag="VNU01_12205"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HWH33423.1"
                     /translation="MSGTPLQDARARRLGDVVAERRIVVCTGAGGVGKTTTAAALALQ
                     AAKAGRRTAVLTIDPARRLAQSLGMPALDNTPRPVHVEGVQTLDAMMLDMKRTFDEVV
                     ERNAGDQARVERIMRNRFYQQLTETLAGTQE"
     gene            complement(397..1362)
                     /locus_tag="VNU01_12210"
     CDS             complement(397..1362)
                     /locus_tag="VNU01_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HWH33424.1"
                     /translation="MDLFGARLLIVTGKGGVGKSTVAAALALAGVRTGRKTCLVEVES
                     RQTMSQLFATQPWDFTEREFRPGLWGLSVDPEASLAEYLDLFYGAGRLSKLVVGSSAV
                     QFATTAAPGVKDVLLIGKVKEMEQRRTADGRFVYDLIVLDAPPTGRIVNFLRAPEAAG
                     ELVGVGPIRQQAQSVVDMLVDPRRTRLELVTLLEEMPVQETLEAVAHLAELGVTLGPV
                     IANRVEPPRVDAAQRKALARASAPAAVAKALAGAGLPGGQDIGRALVGLGAAHAARLD
                     LQAEMLARLDGELEQPVLQLPAIHADRFGPDELNHLADVVEEAGS"
     gene            1428..2759
                     /locus_tag="VNU01_12215"
     CDS             1428..2759
                     /locus_tag="VNU01_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWH33425.1"
                     /translation="MTPRLRLVRRVFAAALLLSGALAGLLSATASAHQGHGVVEILEV
                     RGVIDAPVAGYLLEAVERASSSAHVEALVVELDSPGTLKADVEPLLEAIERSRVPVAV
                     WVPPGGTAAGGALRLLEAAHVQGLALGAVLGPAEPADLSVRAPESSDRVVVVVPEGRD
                     AAALRDWDERLDVVSVSEFDAVEQGYADFVVPTLPDVLRELDGRQVVVDTLGPQTLDV
                     DPVTADVRFQNMGLGRRILHAVSSPALAYVLIVGGALALVFEVFQPGFGVSGITGAAL
                     LGLGGYALTALPVNPLGAVLLLAGLAVLAVDLSLAGFGVLTALGAVSLAAGSVLLVDV
                     PALRLSPWLIAFVVASALVFYVVVMTVVLRAQAGQAQEGADQVVGKRAVVRSMLNPEG
                     HVFVDDALWRARAPEGAGKVRNGTVVRIVGLDDRLTLQVELDDTKADAETS"
     gene            2794..3585
                     /locus_tag="VNU01_12220"
     CDS             2794..3585
                     /locus_tag="VNU01_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWH33426.1"
                     /translation="MEILAVVVPVLVLLLVLIAAAVRIVQEYERGVIFRLGRVIAGAK
                     GPGLFLIVPVIDRMVKVNLQTVTMNVPAQDVITRDNVTVRVDAVIYFNVIDPVKATVN
                     VQNYLFATSQVAQTTLRSVCGTAELDELLSQRDRLNAQVQTVVDELTEPWGVKVTLVE
                     IKDVLLPEPIQRAMARQAEAERDRRAKIISAEGEFQASAKLAEAAAVIAQHPMAYQLR
                     MLQTVTEVAAEKNSTLVLPIPIELLGALKAISEMAPPAGGDASGT"
     gene            3739..4023
                     /locus_tag="VNU01_12225"
     CDS             3739..4023
                     /locus_tag="VNU01_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWH33427.1"
                     /translation="MASLPQLTGAPSTDWQVHGLCRDTDSSVFFAPAQFEHKPDREDR
                     EAKAKAICAECPVRLPCLEWALTTQEPFGVWGGYSEYERKQILVGKRKAS"
     gene            4020..4499
                     /locus_tag="VNU01_12230"
     CDS             4020..4499
                     /locus_tag="VNU01_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWH33428.1"
                     /translation="MSQAGTSPEGRLAALGIVLPEPPAPAAVYVPVVRSGDLLFVAGQ
                     VPFVEGRLQATGKLGGALDLDAGTACARQCAVNVLAQVRAELGSLDHVARVVKLTVFV
                     ASDPSFTEQHLVANGASELFGEVFGDIGRHARSAVGVPVLPMDAPVEVEAVLETGRA"
     gene            complement(4542..6062)
                     /locus_tag="VNU01_12235"
     CDS             complement(4542..6062)
                     /locus_tag="VNU01_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH33429.1"
                     /translation="MLTAPLAAVLLFMTVAVAAPVGAVSQEPPAWAPGQLLVGLEEGA
                     DIEPVLALIDELGGGLVNTDSLRHFQRVCGQATGSGGIVAAQFSGDPAADAARDDLRA
                     ADGVAFVQRAHTVVTTGEAQTESVGEPAGDPTTDPDFAVQWPLRNTGQHLATQWSQTT
                     AAEGWDIDADEAWADGVTGEGQVIAILDDGFSRNHPDLAPNVWVNQAEAEGEPDVDDD
                     GNGWVDDVNGWNVGPENGNYWGGGHGTWVAGIAAARGGNGVGGSGVAPHATLMPVVFI
                     QGTDVNTYSEVNALGAFAYAIGNGADVINNSWGFLAPVGIGSPVMSLAVQCASAHDIP
                     IAWSAGNLGPYPHSFPAYYPADNVVLASVFDEKGCPGVMHPQHPAFADVMAPGSWVHT
                     TSGATGYTYFGQSSAAAPHVAGVMALVRQAHPEYSAQEVVLAIRNGARAPFPEPYNKS
                     LGFLNAAGALEAPVDGLHPHLAASPDPLESTAVRCGVEPAVGIAQAFVDAVTRLLP"
     gene            complement(6183..8045)
                     /gene="selB"
                     /locus_tag="VNU01_12240"
     CDS             complement(6183..8045)
                     /gene="selB"
                     /locus_tag="VNU01_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HWH33430.1"
                     /translation="MRVVCTAGHVDHGKSSLVRALTGMEPDRFAEERRRGLTIDLGFA
                     WADLGGHTVAFVDLPGHERFIANMLAGAGPVETALFVVAADEGWKPQSQEHLDILSLL
                     GTSHGVVAITRADLAPDGGRATADDVAARLAGGPLAGAPVVCCSALTGAGLDELRGAL
                     GDVLTAAPEPREDERPRLWVDRSFTVKGAGTVVTGTLTGGGLRVGEELTLLPGDRRGR
                     VRGLQSLGRQVDAAAPGARVAVNLSGVDRMEARRGDALVRPGHWVETTALEAWVRVLP
                     GERLDRRGAWHLHAGSARRLAAVVPVAGKALEADGFVRLELDEPLALQAGDRFVLREA
                     GRWRTVAGGLALDVRPPAPARGRTRAARVEQLEARRRSVGRPAELLLVHVRERAAARA
                     AGAAAAVGLPLGQAGEAARAHRLLTLGEWLVHAGAAAAWTEASLDALSAHHLRHPMEP
                     GAPRDLVFRAVQDAGCPPELTQAFVDTLVGLGRVVSEGPALRHPAHEATLDQAQAGAR
                     DALLAALDAEPLAPPGLRDAMTAAGAGDALVRELERRGRLVRVAPDLAFTAAAVDAAA
                     ARLREAFPGRPFTASEARQAWGTTRKYAVPLLEELDRRRVTARSGDQRVLSGTS"
     gene            complement(8059..>8488)
                     /locus_tag="VNU01_12245"
     CDS             complement(8059..>8488)
                     /locus_tag="VNU01_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWH33431.1"
                     /translation="FRAVRRMATLDVGRLPVVSAEDHGKLVGLVRRADVVKAYQRAVS
                     KGLGEQQRRASSRLRDLGGTQFIELVVDASSAVAGQPIRAVTWPPRTLVTAVRRHGEI
                     VMPRGDTELVAGDELVVLSGRDTVEELRDLVTGPGRDTGA"
BASE COUNT         1047 a         3134 c         3172 g         1135 t
ORIGIN      
        1 actcctgcgt gccggccagc gtctccgtga gctgctggta gaagcggttg cgcatgatcc
       61 gctccacgcg cgcctggtcc cccgcgttgc gctcgaccac ctcgtcgaac gtgcgcttca
      121 tgtccagcat catcgcgtcg agcgtctgga cgccctcgac gtgcaccggg cgcggcgtgt
      181 tgtccagcgc cggcatgccg agcgactgcg cgaggcgcct ggccgggtcg atcgtcagga
      241 ccgcggtgcg ccgccccgcc ttggcggcct gcagcgccag ggcggcggcc gtggtggtct
      301 tgcccacgcc gcccgcgccc gtgcacacga cgatgcgccg ctcggcgacg acgtcgccga
      361 ggcgccgggc gcgcgcgtcc tgcagcggcg tgccgctcac gagccggcct cctcgacgac
      421 gtcggcaagg tggttgagct cgtccggccc gaagcggtcg gcgtggatgg ccggcagttg
      481 caggaccggc tgctccagct cgccgtccag gcgggcgagc atctcagcct gcaggtccag
      541 gcgcgcggcg tgcgcggcac ccaggccgac cagcgcccgc ccgatgtcct ggccgccggg
      601 cagtcccgcg cccgccagcg ccttggcgac cgccgccggg gcggacgcgc gcgccagggc
      661 cttgcgctgg gcggcgtcca cgcgcggcgg ctccacgcgg ttggcgatca ccggccccag
      721 cgtgacgccg agctcggcga ggtgggcgac ggcttccagg gtctcctgga cgggcatctc
      781 ctccagcagc gtgaccagct ccaggcgcgt gcgccggggg tcgacgagca tgtccacgac
      841 gctctgtgcc tgctggcgga ttggtcccac gccgaccagc tccccggccg cctcgggcgc
      901 gcgcaggaag ttcacgatgc gccccgtggg cggggcgtcc agcacgatga ggtcgtagac
      961 gaagcgcccg tcggcggtcc ggcgctgctc catctccttg accttgccga tgagcagcac
     1021 gtccttgacg ccgggggcgg cggtggtcgc gaactgcacc gcggaggagc cgaccaccag
     1081 cttggacagg cggccggccc cgtagaacag gtccaggtac tccgccaggg aggcctccgg
     1141 gtccaccgac agcccccaca ggcccggccg gaactcgcgc tcggtgaagt cccacggctg
     1201 cgtggcgaac agctgcgaca tggtctggcg gctctcgacc tccaccaggc aggtcttgcg
     1261 gccggtgcgc acgccggcaa gggcgagggc ggcggcgacc gtggacttcc ccacgccgcc
     1321 cttgccggtg acgatcagca ggcgggcgcc gaaaaggtcc aaggtccttg gtgttcggga
     1381 ctggctcacg ggcatcgagt ctcgcccata cccgtacact tttccgcatg acccctcgtc
     1441 tccgccttgt gcggcgggtt ttcgccgctg ccctgctgct gtccggcgcg ctcgccgggc
     1501 tgctgtccgc cacggcctcc gcccaccagg gccacggcgt cgtggagatc ctcgaggtcc
     1561 ggggcgtgat cgacgccccg gtcgccggct acctgctgga ggcggtggag cgcgcgagct
     1621 cgtccgcgca cgtcgaggcg ctggtggtgg agctggacag tcccggcacg ctcaaggcgg
     1681 acgtggagcc gctgctggag gcgatcgagc gcagccgcgt gccggtcgcg gtgtgggtgc
     1741 cgccgggcgg cacggcggcg ggcggcgcgc tgcgcctgct ggaggccgcg cacgtccagg
     1801 gactggccct gggagccgtc ctgggccccg ccgagccggc cgacctgtcg gtgcgggcgc
     1861 ccgagagcag cgaccgggtg gtggtcgtgg tgccggaggg ccgtgacgcg gccgcgctgc
     1921 gcgactggga cgagcggctg gacgtggtgt cggtcagcga gttcgacgcg gtggagcagg
     1981 gctacgccga cttcgtggtg cccacgctgc ccgacgtgct gcgcgagctg gacggccgcc
     2041 aggtcgtggt ggacacgctg ggcccccaga ccttggacgt cgacccggtg accgcggacg
     2101 tgcgcttcca gaacatgggg ctgggccggc gcatcctgca cgcggtgtcc agcccggcgc
     2161 tggcctacgt gctgatcgtg ggcggcgcgc tggcgctggt gttcgaggtc ttccagccgg
     2221 gcttcggcgt ctccggcatc accggtgcgg cgctgctggg gctgggcggc tacgcgctga
     2281 cggcgctgcc ggtcaacccg ctgggcgccg tgctgctgct ggccgggctt gcggtgctgg
     2341 cggtggacct gtccctggcc ggcttcggcg tgctgaccgc gttgggagcc gtgtcgctcg
     2401 cggccggctc ggtgctgctg gtcgacgtgc cggcgctgcg gctgtcgccg tggctgatcg
     2461 cgttcgtggt ggcctccgcg ctggtcttct acgtcgtcgt catgaccgtg gtgctgcgcg
     2521 cgcaggccgg ccaggcgcag gagggcgcgg accaggtcgt gggcaagcgc gccgtggtgc
     2581 gctcgatgct gaacccggag ggccacgtct tcgtggacga cgcgctgtgg cgcgcgcggg
     2641 cgcccgaggg cgccggcaag gtgaggaacg gcacggtggt gcgcatcgtg ggcctggacg
     2701 accgcctgac cctgcaagtg gaactggacg acaccaaggc cgacgccgag acttcctgag
     2761 gtcctcactc gagttcgtcc cgagaggccc gtcatggaaa tcctggcagt cgtggtcccc
     2821 gtcctggtcc tgcttctggt gctgatcgcc gcggcggtcc gcatcgtgca ggagtacgag
     2881 cgcggggtga tcttccggct gggccgggtc atcgcgggcg ccaaggggcc ggggctgttc
     2941 ctgatcgtcc cggtcatcga ccgcatggtg aaggtcaacc tgcagaccgt gaccatgaac
     3001 gtgcccgcgc aggacgtcat cacccgcgac aacgtcaccg tgcgcgtgga cgcggtcatc
     3061 tacttcaacg tgatcgaccc cgtgaaggcg accgtcaacg tgcagaacta cctgttcgcc
     3121 acgtcgcagg tcgcccagac cacgctgcgc agcgtgtgcg gcaccgcgga gctggacgag
     3181 ctgctgagcc agcgcgaccg cctgaacgcc caggtgcaga cggtggtgga cgagctcacc
     3241 gagccctggg gggtcaaggt gacgctggtg gagatcaagg acgtgctgct gcccgagccg
     3301 atccagcgcg cgatggcccg ccaggccgag gccgagcgcg accgccgggc caagatcatc
     3361 agcgccgagg gcgagttcca ggcatcggcc aagctcgccg aggcggccgc cgtgatcgcg
     3421 cagcacccga tggcctacca gctgcgcatg ctccaaacgg tgaccgaggt cgcggcggag
     3481 aagaactcca cgctggtgct gccgatcccg atcgagctct tgggggcgct gaaggccatc
     3541 tccgagatgg cgccgccggc cggcggggac gcgtccggga cctgaccggc tggtccggtc
     3601 gggggtgaaa tccacccctt ttcacgatcc tgcatccgtc gtagcctcgc aggtctccac
     3661 gcctgggacg ggcgccggag accgacatgg acaaccctgg gccgcaggtc ggcggctgaa
     3721 gggccgaggc gaggattcat ggcaagcctg ccgcaactga cgggcgcacc cagcaccgac
     3781 tggcaagtgc atggcctgtg ccgcgacacc gactcgtcgg tgttcttcgc ccccgcgcag
     3841 ttcgagcaca agcccgatcg ggaggaccgg gaggccaaag ccaaggcgat ctgcgccgaa
     3901 tgcccggtgc ggctgccctg cctggagtgg gcgctgacga cgcaggagcc cttcggcgtg
     3961 tggggcggct attcggagta cgagcgcaag cagatcctcg tcggcaagcg caaggcctcg
     4021 tgagccaggc aggaacctcg ccggagggcc gtctcgcggc gctgggcatc gtccttcccg
     4081 agccgcccgc gccggccgcg gtctacgtgc ccgtggtgcg cagcggggac ctgctgttcg
     4141 tggccgggca ggtgcccttc gtcgagggac gcctccaggc caccggcaag ctgggcggcg
     4201 cgctggatct cgatgccggg acggcgtgcg cccggcagtg cgcggtcaac gtgctggcgc
     4261 aggtgcgcgc ggagctcggc tcactcgacc acgtcgcgcg cgtggtcaag ctcaccgtct
     4321 tcgtggcgtc cgacccgtcg ttcaccgagc agcacctcgt cgccaacggc gcgtcggagc
     4381 tgttcggcga ggtcttcggc gacatcggcc ggcacgcccg ctccgcggtc ggtgtgccgg
     4441 tgctgccgat ggacgccccg gtcgaggtgg aggcggtgct ggagacggga cgggcctgag
     4501 gcgcacgccc caggcccgtc cgcgctcccc agtggcggcg tctacggcag caggcgcgtc
     4561 acggcgtcga cgaacgcctg cgcgatcccc acggccggct ccacgccgca gcgcacggcc
     4621 gtggattcca gcgggtccgg gctcgccgcg aggtgcgggt gcaggccgtc caccggcgcc
     4681 tccagcgcgc ccgcggcgtt caggaagccc aggctcttgt tgtacggctc cgggaacggc
     4741 gcccgcgcgc cgttgcgaat cgccaggacc acttcctggg ccgagtactc ggggtgggcc
     4801 tggcgcacca gggccatgac gccggccacg tgcggcgccg ccgcggagct ctgcccgaag
     4861 taggtgtagc cggtggcgcc gctggtggtg tggacccagg agcccggcgc catgacgtcg
     4921 gcgaacgccg ggtgctgggg gtgcatcacg ccggggcagc ccttctcgtc gaagacgctg
     4981 gcgagcacca cgttgtccgc ggggtagtag gccgggaagc tgtgcgggta cgggcccagg
     5041 ttgccggcgg accaggcgat cgggatgtcg tgcgcggagg cgcactggac cgccaggctc
     5101 atgaccgggc tgccgatgcc caccggggcc aggaagcccc agctgttgtt gatgacgtcc
     5161 gcgccgttgc cgatcgcgta ggcgaacgct cccagggcgt tgacctccga gtaggtgttg
     5221 acgtcggttc cctggatgaa caccacgggc atcagcgtgg cgtgcggggc cacgcccgag
     5281 ccgccgacgc cgttgccgcc gcgcgcggcg gcgatgccgg ccacccaggt cccgtgcccg
     5341 ccgccccagt agttgccgtt ctccggcccc acgttccagc cgttgacgtc gtccacccag
     5401 ccgttgccgt cgtcgtccac gtcgggctcc ccctccgcct cggcctggtt cacccacacg
     5461 ttgggcgcca ggtcggggtg gttgcggctg aagccgtcgt ccaggatcgc gatgacctgg
     5521 ccctcaccgg tgacaccatc cgcccacgcc tcgtccgcgt cgatgtccca gccctcggcg
     5581 gccgtggtct gcgaccactg cgtggccagg tgctggccgg tgttgcgcag cggccactgc
     5641 acggcgaagt ccgggtcggt ggtggggtcg ccggcaggct cccccacgct ctccgtctgg
     5701 gcctcgccgg tggtcacgac ggtgtgcgcg cgctgcacga aggcgacgcc gtccgccgcg
     5761 cgcaggtcgt cgcgcgccgc gtcggccgcc gggtcgccgc tgaactgggc ggcgacaatc
     5821 ccaccggaac cggtggcttg gccgcacacg cgctggaagt ggcgcagcga gtccgtgttg
     5881 accaggccgc cgcccagctc gtcgatcagt gcgaggacgg gctcgatgtc ggcgccctcc
     5941 tccaggccca ccagcaactg gccgggcgcc caggccggcg gctcctgcga gacggcccct
     6001 accggggcag ccaccgccac cgtcatgaac agcagcaccg ccgccagcgg ggccgtcagc
     6061 atcctgcgca cgtttcccac cacacactcc cgtgtcgagc ggccccgccg ccgagccact
     6121 acgggtattc cggatgagaa atgacggctg tcacccgaat gggggacgcc atagcgtgac
     6181 agtcaggacg taccggagag cacgcgctga tcgccgctgc gcgccgtgac gcggcggcgg
     6241 tcgagctcct ccaggagcgg caccgcgtac ttgcgggtcg tgccccacgc ctggcgcgcc
     6301 tcgctcgcgg tgaacgggcg gcccgggaag gcctcgcgca ggcgggcggc ggcggcgtcg
     6361 acggccgcgg cggtgaacgc caggtcgggt gcaacccgga ccaggcgccc gcggcgctcc
     6421 agctcgcgga ccagggcgtc gccggcgccc gcggccgtca tggcgtcgcg cagccccggc
     6481 ggtgccaggg gctccgcgtc cagggccgcc aggagggcgt cgcgcgcccc ggcctgcgcc
     6541 tggtccaggg tggcttcgtg ggccgggtgg cgcagcgccg ggccctccga gacgacgcgg
     6601 cccaggccga ccagcgtgtc cacgaacgcc tgcgtgagct ccggcgggca gccggcgtcc
     6661 tgcacggcgc ggaagaccag atccctgggc gcgccgggct ccatcgggtg gcgcagatgg
     6721 tgcgcggaca acgcgtccag cgacgcctcc gtccaggcgg ccgcggcgcc cgcgtggacc
     6781 agccactcgc ccagcgtgag cagccggtgg gccctggcgg cctcgccggc ctgtcccagc
     6841 gggaggccga cggccgccgc ggcgccggcg gcgcgcgccg cggcgcgctc ccgtacgtgc
     6901 acgaggagga gctccgccgg cctgcccacc gagcggcggc gcgcctccag ctgctccacg
     6961 cgcgccgcgc gggtgcgtcc ccgtgccggc gcgggcgggc gcacgtccag cgccaggccg
     7021 ccggcgacgg tgcgccagcg cccggcctcg cgcaggacga agcggtctcc ggcctgcagc
     7081 gccagcggct cgtccagctc caggcgcacg aagccgtcgg cctccagggc cttgcccgcc
     7141 accggcacca cggcggcgag ccggcgcgcg gagccggcgt gcaggtgcca cgcgccacgc
     7201 cggtccaggc gctcgccggg cagcacgcgc acccacgcct ccagcgcggt ggtctcgacc
     7261 cagtggcccg ggcggaccag ggcgtcgccc cgccgcgcct ccatgcggtc cacgccggac
     7321 aggttgacgg ccaccctggc gcccggcgcg gccgcgtcca cctgccggcc caggctctgc
     7381 agcccccgca cgcgcccgcg ccggtcgccg ggcaggagcg tgagctcctc gcccacgcgc
     7441 aggccgccgc cggtgagcgt gccggtcacc accgtgccgg cgcccttcac ggtgaagctg
     7501 cggtccaccc acagccgcgg ccgctcgtcc tcgcgcggct cgggcgccgc cgtcagcacg
     7561 tcgccgagcg cgccgcgcag ctcgtccagg ccggcgccgg tgagggcgct gcagcacacc
     7621 accggcgcac cggccagcgg cccgccggcc agccgcgccg ccacgtcgtc ggcggtggcc
     7681 cggccgccgt ccggggccag gtccgccctg gtgatggcca ccacgccgtg gctggtgccc
     7741 agcagcgaca ggatgtccag gtgctcctgg ctctgcggct tccagccctc gtcggcggcg
     7801 accacgaaca gcgccgtctc caccggcccg gcgccggcga gcatgttcgc gatgaagcgc
     7861 tcgtggccgg gcaggtcgac gaacgccacc gtgtggccgc cgaggtcggc ccacgcgaaa
     7921 cccaggtcga tggtgagccc gcggcggcgc tcctcggcga agcggtccgg ctccatgccg
     7981 gtcagggcgc ggaccaggct ggacttgccg tggtcgacgt ggccggcggt gcagacgacg
     8041 cgcatcacgc ttggcaggct acgcgccagt gtcccggccg gggccggtca cgagatcgcg
     8101 cagctcctcg acggtgtcgc ggccggacag gaccaccagc tcgtcgccgg ccacaagctc
     8161 cgtgtcgccg cgcggcatga cgatctcccc gtggcggcgc accgcggtca ccagcgtgcg
     8221 cggcggccag gtcaccgcgc ggatcggctg gccggcgacg gcgctggagg cgtccacgac
     8281 cagctcgatg aactgggtgc cgcccaggtc gcgcaggcgc gacgacgctc gccgctgctg
     8341 ctcgcccagc cccttggaca ccgcccgctg gtacgccttg accacgtcgg cgcggcgcac
     8401 caggccgacg agcttgccgt ggtcctccgc ggagaccacg ggcaggcggc ccacgtccag
     8461 cgtcgccatg cggcgcaccg cgcggaac
//
LOCUS       DATMKC010000417         4792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_82179, whole genome shotgun sequence.
ACCESSION   DATMKC010000417 DATMKC010000000
VERSION     DATMKC010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82179"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1016
                     /locus_tag="VNU01_12250"
     CDS             75..1016
                     /locus_tag="VNU01_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HWH33432.1"
                     /translation="MPPPRVAIGPADTRDWVRAAVLAGGGQVVAPAEADALIWLGTGY
                     GPDPARGPEGLRALLHGHPGIRWIQLPFAGVEPFAQAGVLTADRVWTAGQGVYARPVA
                     EHALTLALAGLRELPRRVLAREWEAQSGMSLIGGRVTIFGGGGIARELIGLLAPFDCD
                     ITVVRRRPEPVPGAARTLGFGERYEALKGADVVVLALALTAETDGLVGAVELELMEPH
                     AWLVNVARGRHVVTEDLVDALREGAIGGAGLDVVEPEPLPPGHPLWSLPNCILTPHTA
                     NTEAMGQPLLAMRIRENVQRFGSGQPLLGLVDPELGY"
     gene            1016..2206
                     /locus_tag="VNU01_12255"
     CDS             1016..2206
                     /locus_tag="VNU01_12255"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWH33433.1"
                     /translation="MRRLAAVAARHWRATLLIWALLVAVSVAAAPDFTEVATYDDNAF
                     LPTGADSVRGAELMRQGWPDDNFTSSAVIALLRDDGPLTQADRAVAGRLVEWLPSDAA
                     PTGLGRTVTHLDEPDLEDSLTSKDGHAMLLVAGMEVPPFSPMGHEVVTELRRHIAETD
                     RPEGLDVMVTGAAGIALDENAAIEASVKRTELLTVLLVVVLVLWVLRSPVAALVPLVT
                     VAGAYAVALAVVGTLARLGMDVSYLFQMFAIVIVFGAGTDYALLILARYQEELADSPD
                     AAEAEGRARRLAGTMVVLAGVLASTAASTMVGFSAQVVAQFGLFRTMGPALAIAVLIT
                     LLAGLTLTPAIMRLCGRWLFWPGGLVTGKGMRAGHGPGGGDPVWEIVPADEGPADGAR
                     GDAR"
     gene            2203..4182
                     /locus_tag="VNU01_12260"
     CDS             2203..4182
                     /locus_tag="VNU01_12260"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWH33434.1"
                     /translation="MRAPAWIARRLPPGDGVWDRVATQVTTRPGATLLLSLLVVSLPL
                     LALPGLRTTHDVLSSLPQDAESVAAQRRHLPAGETSPLILVIDGDESVYQPVAFRALG
                     DLSKNLRRLPGVASVRSVAMPTDGERPEIDPALEGQAGDLAGQVDQAAAGAAQIAAGV
                     QELETGLRTIDARLPELRDGLAEGRDGAARLHDGVVQARSGVRQVRAGLARLRDGLGE
                     AQDGAARLRDEVARPTERELAEAIDGLATMTVGRTDPMYQEVYESVGEAYARVTGRYP
                     VGHERAGQRVDPAYGGLTASLDELAAGLGQARSGADRLDGGLAQLETGLGQAVDGLRR
                     LRDGLGDGVEGVAQLQEGIERMLDGVAGRLRPGTQRLAEGLAEGSRALEESGVGELIL
                     GGEGPFVLTPGMLDIVPELRERLSFFTAEEGTRTRVFIGLASDPFSDEAIATARLARE
                     VAERSLLDSPLADADVVPTGSAAFVSEIDRAAAQDFPVIVAAVSIGVFLVLVALLRSL
                     VAPLYMVLTVLLSYASVMGLTALVFQRLLGEPGVQWWVPPILFVLLVGLGVDYSIFLM
                     GRVREEAQRRITRDAVAEGVRTTGRVITSAGIILAGTFAALIAAPMRSMTQLGFAAAA
                     GILLDTFLVRALLVPSLAVLLGRWNWWPSARASAD"
     gene            4183..>4792
                     /locus_tag="VNU01_12265"
     CDS             4183..>4792
                     /locus_tag="VNU01_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33435.1"
                     /translation="MEIGRLALRGAGGEPVDLARTVHAHGCTTLPPSEVSLDPPALEA
                     VVRPARGKPRRIRVAGRRGQAVVEAFGPAPSAAMARELRSLAARLLRLDADLSGFYLL
                     AADDPELAWVTAGAGRIARSASPYEDLVKTLLTTNCAWSATVRMTTALVEHLGEPAVG
                     GGHAFPAPAVMAEAGEDFYRQVVRAGYRAKSLRAVATLVAEGA"
BASE COUNT          519 a         1758 c         1876 g          639 t
ORIGIN      
        1 gccgaggtgg tcgacgacat cgcgcgggcc atcggcgcca cggacgcgcg cggcctggat
       61 ctcgcgtagc ggcgatgccg ccgccccgcg tcgccatcgg ccccgctgac acccgcgact
      121 gggtgcgcgc cgccgtcctc gccggcggcg ggcaggtcgt cgcgccggcc gaggccgacg
      181 ccctgatctg gctcggcacc ggctacggcc ccgatccggc caggggcccg gagggcctgc
      241 gcgcgctgct gcacggccat cccggcatcc gctggatcca gctgccgttc gccggcgtgg
      301 agccgttcgc ccaggcgggc gtgctcaccg ccgaccgcgt gtggaccgcc ggccagggcg
      361 tgtacgcgcg ccccgtggcc gagcacgcgc tgacgctggc gctggcggga ctgcgcgagc
      421 tgccgcgccg cgtgctggcg cgcgagtggg aggcgcagtc cggcatgagc ctcatcggcg
      481 gccgcgtcac catcttcggc ggcggcggca tcgcccgcga gctgatcggc ctgctggccc
      541 cgttcgactg cgacatcacg gtcgtgcgcc ggcgtcccga gccggtgccc ggggccgccc
      601 gcacgctggg cttcggcgag cgctacgagg cgctcaaggg cgccgacgtc gtggtgctcg
      661 cgctggcgct gaccgccgag acggacgggc tcgtcggcgc ggtggagctg gagctgatgg
      721 agccgcacgc gtggctggtc aacgtggcgc gaggccgcca tgtggtcacc gaggacctgg
      781 tggacgcgct gcgcgagggc gcgatcggcg gcgccggcct ggacgtggtg gagcccgagc
      841 ccctgccgcc cggccacccg ctgtggtcgc tgccgaactg catcctcacg ccgcacacgg
      901 ccaacacgga ggccatgggc cagccgctgc tggccatgcg catccgcgag aacgtccagc
      961 gcttcggctc cggccagccc ctgctgggcc tggtcgaccc ggagctgggc tactgatgcg
     1021 ccggctggcg gccgtggccg cccggcactg gcgcgccacg ctgctgatct gggccctgct
     1081 cgtggcggtg tccgtggcgg cggcgccgga tttcaccgag gtcgcgacct acgacgacaa
     1141 cgcgttcctg cccaccggcg cggactccgt gcgcggtgcg gagctgatgc ggcagggctg
     1201 gccggacgac aacttcacct cgtcggccgt gatcgcgctg ctgcgcgacg acgggccgct
     1261 cacccaggcg gaccgggcgg tcgccgggcg cctggtcgaa tggctgccct cggacgcggc
     1321 cccgaccggg ctggggcgca cggtcaccca cctggacgag ccggacctgg aggactcgct
     1381 cacctcgaag gacggccacg cgatgctgct cgtggccggc atggaggtgc cgccgttcag
     1441 ccctatggga cacgaggtgg tcacggagct gcgccgccac atcgccgaga cggaccgccc
     1501 ggagggtctc gacgtgatgg tgacgggcgc ggccggcatc gcgctggacg agaacgccgc
     1561 catcgaggcc agcgtcaagc gcacggagct cctcaccgtg ctcctcgtcg tggtgctcgt
     1621 gctgtgggtg ctgcgctcgc ccgtggcggc gctggtcccg ctcgtcaccg tggccggcgc
     1681 gtacgccgtc gcgctggccg tcgtcggcac gctggcgcgc ctcggcatgg atgtgtccta
     1741 cctgttccag atgttcgcca tcgtgatcgt gttcggcgcg ggcaccgact acgcgctgct
     1801 gatcctcgcc cgctaccagg aagagctcgc ggacagcccc gacgcggccg aggcggaggg
     1861 ccgcgcccgg cgcttggcgg gcaccatggt ggtgctggcc ggcgtgctcg cctccaccgc
     1921 ggcctccacg atggtgggct tcagcgcgca ggtggtggca cagttcgggc tgttccggac
     1981 gatggggccg gcgctcgcga tcgccgtgct gatcaccctg ctcgccgggc tcacgctcac
     2041 acccgcgatc atgcggctgt gcgggcgctg gctgttctgg ccggggggcc tggtgaccgg
     2101 caagggcatg cgcgccggcc acgggcccgg cggcggcgac ccggtctggg agatcgtgcc
     2161 cgccgacgag gggccggccg acggggcgcg gggtgacgcc cgatgagggc gcccgcgtgg
     2221 atcgcccgcc ggctgccccc aggcgacggc gtctgggacc gcgtcgccac gcaggtcacc
     2281 acccggcccg gcgcgacgct gctgctcagc ctgctggtgg tgtccctgcc gctgctggcg
     2341 ctgccggggc tgcgcaccac ccacgacgtg ctgtcgagcc tgccccagga cgccgagtcg
     2401 gtggcggcgc agcgccgcca cctgcccgcc ggggagacct cgccgctgat cctggtgatc
     2461 gacggtgacg agtccgtgta ccagccggtc gccttccgcg cgctcggcga cctgtccaag
     2521 aacctgcggc ggctgcccgg cgtggccagc gtccgctcgg tggcgatgcc gacggacggc
     2581 gagcgcccgg agatcgaccc cgcgctggag ggccaggccg gcgacctcgc cgggcaggtc
     2641 gaccaggccg cggccggcgc cgcgcagatc gccgccggcg tgcaggagct ggagacgggc
     2701 ctgcgcacga tcgacgcgcg cctgccggag ctgcgcgacg ggctggccga gggccgcgac
     2761 ggcgccgcgc gcctgcacga cggggtggtg caggcacgca gcggcgtgcg ccaggtccgt
     2821 gccggcctcg cgcgcctccg cgacggcctc ggggaggcac aggacggcgc ggctcgcctg
     2881 cgcgacgagg tggcccgccc caccgagcgg gaactggcgg aggcgatcga cggcctggcc
     2941 accatgaccg tcggccgcac cgacccgatg taccaggagg tctacgagtc cgtcggcgag
     3001 gcctacgcgc gggtcacggg ccgctatccg gtgggtcacg agcgcgccgg tcagcgcgtc
     3061 gacccggcgt acggcggcct caccgcgtcc ctggacgagc tggccgccgg gctgggccag
     3121 gcgcgctccg gcgcggaccg gctcgacgga gggctcgccc agctggagac gggcctgggt
     3181 caggcggtgg acgggttgcg gcgcctgcgc gacgggctcg gggacggcgt cgaaggggtg
     3241 gcgcagctgc aggagggcat cgagcgcatg ctggacggcg tggccggccg gctgcgcccg
     3301 ggcacgcagc ggctggccga gggcctggcg gagggctctc gggcgctgga ggagtccggc
     3361 gtcggcgagc tgatcctggg cggcgagggc ccgttcgtgc tcacccccgg catgctcgac
     3421 atcgtcccgg agctgcgcga gcggctgtcc ttcttcaccg ccgaggaggg cacgcgcacg
     3481 cgcgtcttca tcgggctggc gtccgacccg ttcagcgacg aggccatcgc cacggcaagg
     3541 ctggcgcgcg aggtggccga gcggagcctc ctggactcgc cgctggcgga cgcggacgtg
     3601 gtgccgaccg ggtccgcggc gttcgtgagc gagatcgacc gggccgccgc gcaggacttc
     3661 ccggtcatcg tggccgccgt cagcatcggc gtcttcctgg tgctggtggc gctgctgcgc
     3721 tccctggtcg cgccgctgta catggtcctg accgtgctgc tgagctacgc ctcggtgatg
     3781 ggcctgaccg cgctggtgtt ccagcggctg ctcggcgagc cgggcgtgca gtggtgggtg
     3841 ccgcccatcc tgttcgtgct gctggtcggc ctcggcgtgg actactcgat cttcctcatg
     3901 ggccgcgtgc gcgaggaggc gcagcggcgc atcacgcgcg acgccgtggc cgagggcgtg
     3961 cgcaccaccg gccgtgtcat cacctcggcc gggatcatcc tggcgggcac gttcgccgcg
     4021 ctgatcgccg cgccgatgcg gtccatgacc cagctcggct tcgcggccgc cgcgggcatc
     4081 ctgctggaca cgttcctggt gcgcgcgctg ctggtgccgt cgctggccgt gctgctgggc
     4141 cgctggaact ggtggccctc ggcccgcgcg agcgccgact agatggagat cgggcggctg
     4201 gcgctgcgcg gggcgggcgg agagcccgtg gacctggcgc gcaccgtgca cgcgcacggc
     4261 tgcaccacgc tgccgccgag cgaggtgtcg ctggacccgc cggccctgga ggccgtggtg
     4321 cggcccgcgc gcggaaagcc gcggcgcatc cgcgtcgccg ggcggcgcgg ccaggcagtg
     4381 gtcgaggcgt tcggcccggc gccatcggcc gcgatggccc gcgagctgcg gtcgctcgcc
     4441 gccaggctgc tgcgcctgga cgcggacctg tccggcttct accttctggc cgcggacgac
     4501 ccggagctgg cctgggtgac cgcgggcgcg ggccgcatcg cgcgcagcgc cagtccctac
     4561 gaggacctgg tcaagaccct cctgaccacc aactgcgcgt ggtcggcgac cgtgcgcatg
     4621 acgaccgcgc tggtcgagca cctgggcgag cccgccgtcg gcggcggcca cgccttcccg
     4681 gctccggccg tcatggccga ggccggcgag gacttctacc ggcaggtggt gcgcgccggc
     4741 taccgcgcga agtcgctgcg cgccgtcgcg accctggtcg cggagggggc gc
//
LOCUS       DATMKC010000418         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_135766, whole genome shotgun sequence.
ACCESSION   DATMKC010000418 DATMKC010000000
VERSION     DATMKC010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135766"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VNU01_12270"
     CDS             <1..1488
                     /locus_tag="VNU01_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33436.1"
                     /translation="EQVCPAERLAVSWREPDDSYQWGAHVEPIGPAPGAATTAVNGVV
                     LCQDAGFAFMGFEAWRADGTWQLAAVPYVREEGEAHPDEAPGGDHPHRPAPAEPAPAP
                     PAPARPATPPALSLDLPSGSIEGYAAYEPQRLCDPGPKPGTLALRNLLLGTNPGTRNL
                     GIGRSCGAGGQSEHKEGRALDWGVLVSRPAERAAADRFVAQLMATDAQGNPHAMARRM
                     GVMYVIWNRQIWSAYRPAAGWRAYGGPSPHTDHVHVSLSWAGAMGRTSFWSGKVDPAT
                     LLAHQPVPASGGSAGSGGTGSGGGSGTHQPIPASLPTEAVRDAVRERRKDRRGDHVTE
                     PRPETATEAGTEAEAARRAEREQRRRERFDERAERRALRAAERAKRHADEAERQAAER
                     QEAERQEAERQAAEAEAERREAAATAPRHRGEQRPGGNGNGQAEAERAERAERNGERQ
                     RKAQVKAERRQERKAEADGTRGGKGHGTRGGKGHGKGKGGAKGRD"
     gene            complement(1510..>2207)
                     /locus_tag="VNU01_12275"
     CDS             complement(1510..>2207)
                     /locus_tag="VNU01_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWH33437.1"
                     /translation="RADDVLPGPVPVLGRLWQELVGGTLLQATGVTMARGLVGYGIAV
                     MLGVAAGLAITRSRALRSAVGSLLTALQTLPSIAWFPLALLLFPESESAILVVVVLGA
                     APAIANGLLHGVDHIPPLLLRAGKVLGASGVAAYRHVILPAALPSFTAGLKQGWAFAW
                     RGLMAGELLVVVAGRPSLGLALQVSREAGDAVALLAVMVAILIVGIAVDGLLFSPLER
                     TVRRRWGLADAAR"
BASE COUNT          330 a          888 c          823 g          166 t
ORIGIN      
        1 gagcaggtct gcccggccga gcgcctggcc gtgtcctggc gggagccgga cgactcctac
       61 cagtggggcg cgcacgtcga gcccatcggc ccggcccccg gagcggcgac caccgccgtc
      121 aacggcgtgg tgctgtgcca ggacgccggc ttcgccttca tgggcttcga ggcctggcgc
      181 gccgacggga cgtggcagct cgcggccgtc ccgtacgtgc gcgaggaagg cgaggcgcac
      241 cccgacgagg cgcccggcgg cgaccacccg caccgcccgg caccggccga gccggcgccc
      301 gcgccgcccg cccccgcacg gcccgcgacg ccgcccgcgc tgtccctgga cctgccgtcg
      361 ggcagcatcg agggctacgc ggcctacgag ccgcagcgcc tgtgcgaccc gggaccgaag
      421 ccgggcacgc tggcgctgcg caacctgctg ctgggcacca acccgggtac ccgcaacctg
      481 ggcatcggcc gctcctgcgg ggccggcggg cagtccgagc acaaggaggg ccgcgccctg
      541 gactggggcg tgctggtgag ccgcccggcc gagcgcgccg cggccgaccg cttcgtcgcc
      601 cagctcatgg ccaccgacgc gcagggcaac ccgcacgcca tggcacgccg catgggcgtc
      661 atgtacgtga tctggaaccg ccagatctgg tcggcctacc gcccggcggc gggctggcgc
      721 gcctacggcg ggcccagccc gcacaccgac cacgtgcacg tctcgctgtc gtgggccggg
      781 gccatgggcc gcaccagctt ctggtccggc aaggtcgacc ccgccacctt gctggcccac
      841 cagcccgtcc cggcgtccgg cggctccgcg ggcagcggcg gcaccggcag tggcggcggc
      901 tccggcaccc accagcccat ccccgcgtcg ctgccgaccg aggcggtgcg cgacgccgtc
      961 agggagcggc gcaaggaccg ccgcggcgac cacgtcaccg agccccggcc cgagaccgcg
     1021 acggaggccg ggacggaggc cgaggcggcg cggcgcgccg aacgcgagca gcgccgtcgg
     1081 gagcgcttcg acgagcgcgc ggaacggcgc gcgctgcgcg ccgccgagcg cgccaagcgc
     1141 cacgccgacg aggccgagcg ccaggcggcc gagcgccagg aagcggagcg ccaggaagcg
     1201 gagcgccagg cggcggaggc agaggcggag cgccgggaag ccgcggccac cgcgccccgg
     1261 caccgcggcg aacagcgtcc cggaggcaac ggcaacggcc aggccgaggc cgagcgcgcg
     1321 gagcgcgccg agcgcaatgg cgagcgacag cgcaaggcgc aggtgaaggc cgagcgacgc
     1381 caggagcgca aggccgaggc cgacggcacg cgcggcggca agggccacgg cacgcgcggc
     1441 ggcaagggcc acggcaaggg caagggcgga gccaagggac gcgactgagc ccccgcggcg
     1501 ccgcgggggt cacctcgcgg cgtcggccag gccccagcgc cggcgcaccg tccgctccag
     1561 cgggctgaac agcagcccgt ccacggcgat gcccacgatc aagatcgcca ccatgacggc
     1621 gagcagcgcc acggcgtcgc cggcttcccg agacacctgc agcgccaggc cgagcgacgg
     1681 acggccggcg accaccacga gcagttcgcc cgccatgagc ccgcgccacg cgaacgccca
     1741 gccctgcttg aggcccgccg tgaacgacgg cagcgccgcc ggcaggatca cgtggcggta
     1801 cgccgcgaca ccgctcgccc cgagcacctt gccggcgcgc agcagcagcg gcgggatgtg
     1861 gtcgacgccg tgcagcagcc cgttcgcgat ggcgggcgcg gcgcccagca ccaccaccac
     1921 caggatcgcc gactcagact ccgggaacag cagcagcgcc agcgggaacc acgcgatcga
     1981 cggcagcgtc tgcagcgccg tcagcagcga ccccacggcg ctgcgcagcg cccggctgcg
     2041 cgtgatcgcc agccccgcgg ccacgccgag catcaccgcg atgccgtagc ccaccagccc
     2101 gcgcgccatc gtcacacccg tggcctgcag cagcgtgccg cccaccagct cctgccacag
     2161 ccggcccaac acggggaccg ggccgggcag cacgtcgtcg gcgcgcc
//
LOCUS       DATMKC010000419         4035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_334763, whole genome shotgun sequence.
ACCESSION   DATMKC010000419 DATMKC010000000
VERSION     DATMKC010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334763"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VNU01_12280"
     CDS             <1..978
                     /locus_tag="VNU01_12280"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002190.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWH33438.1"
                     /translation="PQTAPLTAPLTVLLAAPRGFCAGVERAVEIVELALRTYGPPVYV
                     RHQIVHNTHVVADLEAKGAVFVEDEAEVPEGGIVVFSAHGVAPQVKANAETLGLRAID
                     ATCPLVTKVHIEARDYAERGYSIVLIGHEGHQEVVGTMGNAPESITLVETPEDVASLD
                     LPDPAKVAYLSQTTLSVDEANRVIEALKARYPLARGPKGDDICYATQNRQDAVKVLAE
                     RADLVLVVGSENSSNSKRMVEVALESGARDARLIDDVRDIEDAWLDGVRSVGLTSGAS
                     APEVLVGEVIERLRARPGGAVVETVQVVQEDMHFALPAGLRRLDVVETG"
     gene            complement(975..1265)
                     /locus_tag="VNU01_12285"
     CDS             complement(975..1265)
                     /locus_tag="VNU01_12285"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWH33439.1"
                     /translation="MADLLDADGLRQGLEILEGWDGNLGGIEKTWTFTDFDEAVRFVV
                     RVADVVRVPNHHPDVDIRWNKVTLKLVSHSAGGLTQADIDTAALIDRETEGF"
     gene            complement(1258..2469)
                     /locus_tag="VNU01_12290"
     CDS             complement(1258..2469)
                     /locus_tag="VNU01_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HWH33440.1"
                     /translation="MALERYEVADTPDRLSHALAELSQLEQVGVDVERADWDRYWRRA
                     ALIQVGGEGIVVLVDPMVLESLEELDAFLAGRLTVLHAMENDLPPLASAGVQPDRIAD
                     TAIAAGLLGLPTGLESLLAELLDVRLEGDKAAMQRADWERRPLTEDMLRYAAGDVADL
                     PALWRELEDRLHEAGRMDWYRQELDAAIALPAVHDRRDWTRTKGAGRLDGPARARLRV
                     LWDTREELARDHDTAPGRIMPDKLMIDLAGSPAGSPPELVRRGMRRQAARDHGERIVA
                     ALRAGHEAAAEPAARTHRPITDADRALVDKLRVRRAELARGVGIDPGILCPSRTLMNA
                     VVADPPDPATLRELLGLRPWQWEILGWDFCEILELDGPGMPERPAVDPAGTADDAEPP
                     PTTDDGSDTNG"
     gene            complement(2479..3330)
                     /locus_tag="VNU01_12295"
     CDS             complement(2479..3330)
                     /locus_tag="VNU01_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH33441.1"
                     /translation="MTFPGGSGWQRGEARASSGVLAYRRGGGGTPLLYLHDAGADTVG
                     SPAFDDLARDHDVVVLDLPGYGGSTPPGPMSSPARMADLLAELLDALGWTAAVVAGSS
                     LGGWFGMELAIRHPSRVRALVLADPAGLHCPEDYLFALFCQGRAAASAQRLIAEAIWE
                     RLPADEGLAEGGDVGAAVWGPWVQELAAAAWCSWHPYTQNPRLLALAGRIRCPVHILW
                     GARDALIPLRHGRVLAAVVPGARLEILAGAGHLPVLDAPERFAAAVRGVSSGPTDPDP
                     PERLAVG"
     gene            3398..>4035
                     /locus_tag="VNU01_12300"
     CDS             3398..>4035
                     /locus_tag="VNU01_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWH33442.1"
                     /translation="MAEQAPGLMCVHAHPDDEVIMTGGVLARAVSEGIRARVVTCTGG
                     EMGEVVGEGMDPAEIFPKLGEVRLEELREALSILGTDGPQMLGYRDSGMMGTDGNADP
                     RSFWQADMDEAVERLVRQIREFRPTVLVSYDAFGGYGHPDHIQAHRVMFLASEAAAVP
                     MLYPDAGPAWPVRKVYFATLPKSRIFLVNQLMAAQGLASPFPTAERAEDLPMG"
BASE COUNT          537 a         1462 c         1519 g          517 t
ORIGIN      
        1 ccccagacgg cgcccctgac ggcgcccctg acggtgctgc tcgccgcgcc gcgcggcttc
       61 tgcgccggcg tggagcgcgc cgtggagatc gtcgagctgg cgctgcgcac gtacggcccg
      121 cccgtgtacg tccgccacca gatcgtgcac aacacccacg tggtcgccga tctggaggcc
      181 aagggcgcgg tgttcgtgga ggacgaggcg gaggtgcccg agggcggcat cgtggtgttc
      241 agcgcgcacg gcgtggcccc gcaggtcaag gccaacgcgg agacgctggg cctgcgcgcc
      301 atcgacgcga cctgcccgct ggtcaccaag gtccacatcg aggcgcgcga ctacgccgag
      361 cgcggctact cgatcgtgct gatcggccac gagggacacc aggaggtggt cggcacgatg
      421 ggcaacgcgc cggagtcgat cacgctggtg gagacgccgg aggacgtggc gtcgctggac
      481 ctgccggacc cggccaaggt ggcctacctg tcgcagacca cgctgtcggt ggacgaggcc
      541 aaccgcgtga tcgaggcgct caaggcgcgc tacccgctgg cacgggggcc caagggcgac
      601 gacatctgct acgcgacgca gaaccgccag gacgccgtga aggtgctcgc cgagcgcgcc
      661 gacctggtcc tggtggtggg ctcggagaac tcctccaact ccaagcgcat ggtggaggtg
      721 gcgctggaga gcggcgcccg cgacgcgcgc ctcatcgacg acgtgcgcga catcgaggac
      781 gcctggctgg acggcgtgcg cagcgtcggg ctgacgtccg gcgccagcgc cccggaggtg
      841 ctggtgggcg aggtcatcga gcgcctgcgc gcccggcccg gcggcgcggt cgtggagacc
      901 gtgcaggtgg tgcaggagga catgcacttc gccctgcccg cggggctgcg ccgcctggac
      961 gtggtggaga cgggctagaa gccttcggtc tcgcggtcga tcagcgccgc ggtgtcgatg
     1021 tcggcctgcg tcagcccgcc ggcggagtgg ctcaccagct tcagggtcac cttgttccag
     1081 cggatgtcga catcggggtg gtgattcggc acgcgcacga cgtcggcgac gcgcaccacg
     1141 aagcgcaccg cctcgtcgaa gtcggtgaac gtccaggtct tctcgatgcc gccgaggttg
     1201 ccgtcccacc cctccaggat ctccaggccc tgtcgcagtc cgtcggcgtc cagcaggtca
     1261 gccattcgtg tcacttccgt cgtcggtggt cggtggcggc tcggcgtcgt ccgccgtgcc
     1321 ggcgggatcg acggccgggc gctcgggcat gccggggccg tcgagctcca ggatctcgca
     1381 gaaatcccat cccaggatct cccattgcca cggtcgcaat cccagcagtt cgcgcaaggt
     1441 ggccgggtcc ggcgggtcgg cgaccacggc gttcatgagc gtgcggctgg gacacaggat
     1501 gccggggtcg atgcccaccc cgcgcgccag ctcggcgcgg cgcacgcgca gcttgtcgac
     1561 cagcgcgcgg tccgcgtcgg tgatgggacg gtgggtccgg gcggcgggct cggcggccgc
     1621 ctcgtgcccg gcgcgcagcg ccgcgacgat gcgctcgccg tggtcgcgcg ccgcctggcg
     1681 gcgcatcccg cggcggacca gctccggcgg ggacccggcc gggctgccgg cgaggtcgat
     1741 catgagcttg tccggcatga tccgcccggg cgcggtgtcg tggtcgcggg ccagttcctc
     1801 ccgggtgtcc cacagcacgc gcaggcgggc gcgcgccggc ccgtcgagcc ggccggcgcc
     1861 cttggtgcgg gtccagtcgc ggcggtcgtg cacggcgggc agggcgatcg cggcgtccag
     1921 ctcctggcgg taccagtcca tccgccccgc ctcgtgcagg cggtcttcca gctcgcgcca
     1981 cagcgccggc aggtcggcga cgtcaccagc ggcgtagcgc agcatgtcct cggtcagtgg
     2041 ccggcgctcc cagtcggcgc gctgcatggc cgccttgtcg ccctccaggc gcacgtcgag
     2101 cagttccgcg agcaggctct ccagcccggt gggcaggccc agcagtcccg ccgcgatcgc
     2161 ggtgtcggcg atgcggtcgg gctgcacgcc ggcggacgcc agcggcggca ggtcgttctc
     2221 catggcgtgg agcacggtga ggcgcccggc caggaacgcg tccagttcct ccagcgactc
     2281 cagcaccatc gggtcgacca gcaccacgat gccctcgccg cccacctgga tcagggccgc
     2341 ccgccgccaa tagcggtccc agtcggcccg ctcgacgtcc acaccgacct gctcgagctg
     2401 ggagagctcg gcgagcgcgt ggctcaggcg gtcgggagtg tccgcgacct cgtagcgctc
     2461 cagtgccaca cgcacacctc atcccaccgc cagtcgctct ggcgggtccg gatccgtcgg
     2521 cccggaggat acgcctcgga ccgcggcggc gaagcgctcc ggcgcgtcca gcaccggcag
     2581 gtgcccggcg cccgccagga tctccagccg cgcgccgggc acgacggcgg ccaggacgcg
     2641 cccgtgccgc agcgggatga gggcgtcacg ggcgccccac aggatgtgga ccgggcagcg
     2701 gatgcggccg gccagcgcca gcaggcgcgg gttctgcgtg tacgggtgcc agctgcacca
     2761 cgccgccgcg gcgagctcct gcacccacgg gccccagacg gccgcgccga cgtcgccgcc
     2821 ctcggccagt ccctcgtcgg cgggcaggcg ctcccagatg gcctcggcga tgaggcgctg
     2881 ggcgctcgcg gccgcccggc cctggcagaa cagcgcgaac aggtagtcct ccgggcagtg
     2941 cagccccgcg gggtcggcga gcaccagcgc gcgcacccgt gacggatggc ggatggccag
     3001 ctccatgccg aaccagccgc ccaggctgga gccggcgacc accgccgccg tccagcccag
     3061 cgcgtccagc agctccgcca gcaggtccgc catccgtgcc ggcgagctca tgggcccggg
     3121 tggcgtggag ccgccgtagc cgggcaggtc caggaccacg acgtcgtggt cgcgggccag
     3181 gtcgtcgaag gcgggggagc cgaccgtgtc ggcgccggcg tcgtgcaggt acagcagcgg
     3241 cgtaccgccg ccgccgcgcc ggtacgccag cacgccggaa gaagcgcggg cctcgccgcg
     3301 ttgccagccg gagccgccgg ggaaggtcat gggcgccgcc catcctgccc cagcgcgcgg
     3361 ctaggctccc tgaccagtgc cgaccaagga gagggacgtg gcggaacagg cacccggact
     3421 gatgtgcgtg cacgcgcacc ccgacgacga ggtgatcatg acgggcggcg tgctcgcgcg
     3481 cgccgtgtcg gagggcatcc gcgcgcgcgt ggtcacgtgc acgggcgggg agatgggcga
     3541 ggtcgtgggc gaggggatgg accccgccga gatcttcccc aagctcggcg aggtgcgcct
     3601 ggaggagctg cgcgaggcgc tgtccatcct gggcaccgac gggccgcaga tgctcggcta
     3661 ccgcgactcc gggatgatgg gcaccgacgg gaacgccgac ccgcgcagct tctggcaggc
     3721 cgacatggac gaggccgtgg agcggctggt gcgccagatc cgggagttcc gcccgaccgt
     3781 gctggtcagc tacgacgcat tcggcggcta cggccatccc gaccacatcc aggcgcaccg
     3841 ggtgatgttc ctggcctcgg aggccgcggc ggtgcccatg ctgtacccgg acgccgggcc
     3901 ggcttggccg gtgcgcaagg tgtacttcgc gacgctgccg aagtcgcgca tcttcctggt
     3961 caaccagctc atggcggcac aggggctggc ctcgccgttc ccgaccgccg agcgcgccga
     4021 ggacctgccc atggg
//
LOCUS       DATMKC010000420         2471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_178472, whole genome shotgun sequence.
ACCESSION   DATMKC010000420 DATMKC010000000
VERSION     DATMKC010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178472"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VNU01_12305"
     CDS             complement(<1..887)
                     /locus_tag="VNU01_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH33443.1"
                     /translation="MTRPIRIGVVDDHGRVHEALRQVLDAQPGFQVVGGAQDPDGAVT
                     LVETHEPDLVLMDVRMPGGGGAEATRRIREHNPLTRVVALSAHEDRISVLEMLRAGAV
                     GYVTKGAPVEELVSAIRSAVEGGRPLSASVTVEVAAELAERLSWEEYDARHSRRQRAL
                     LEAALRVGVRPVYQPIFDLASGALAGLEALARFPADPAVPVPVWFAAAAEAGLGPELE
                     LAAIRAALAGARDRPRDVYLAVNVSPACLGAPVVVEALTQTDLSGVVLELTEHARVTD
                     YGGLAELLAPLRAGGARPQA"
     gene            1255..1869
                     /locus_tag="VNU01_12310"
     CDS             1255..1869
                     /locus_tag="VNU01_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWH33444.1"
                     /translation="MRVLLVEDHAVVREGLAAVLEHSGAISIAGQASSVRDGLRILER
                     EAVDVVVLDLRLGDEHGVDLVREVRRREDGPAILVLSAHDATLELERALGAGANGYLL
                     KGSDAVTVTDAVLETAAGRTVIDTGFVPKLLHRAAHMEDQPTPRELEVLRLCAEGATA
                     VEIARELGISPRTAQKHIERLFKKLGVSNRAELVSAAFRRGLVR"
     gene            complement(1866..>2471)
                     /locus_tag="VNU01_12315"
     CDS             complement(1866..>2471)
                     /locus_tag="VNU01_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33445.1"
                     /translation="HRQRVHRGDRDALLLGAAVAAMHTLSVLVLGVALHTLAAGGSAL
                     ERAGPWLTLVAGLLVLAVGAASLGRVLRHRRATAEPGWHDHAALPAGVSPLSRRGLVL
                     IGMSGGLLPSPSAFLVLTTALFTGRTLFGLALVAAFSMGLAASLALVGLAVLRGRDLA
                     VTRLAGSPRLVAALDALPLASAVLLTVAGAWLSATAASRLA"
BASE COUNT          343 a          937 c          934 g          257 t
ORIGIN      
        1 gcctgcgggc gggcgccgcc cgcccgcagg ggcgcgagca gttcggccag gccgccgtag
       61 tcggtgaccc gcgcgtgctc ggtgagctcc aggaccacgc cggacaagtc cgtctgggtg
      121 agcgcctcca ccaccaccgg cgcgcccagg caggccggcg agacgttcac cgccaggtac
      181 acgtcgcgcg ggcggtcgcg cgcgccggcg agcgccgccc ggatggccgc cagctccagc
      241 tccgggccca gccccgcctc ggcggcggcg gcgaaccaca ccggcaccgg gacggccggg
      301 tcggcgggga agcgggccag cgcctccagg cccgccagcg ccccggaggc caggtcgaag
      361 atcggctggt acaccgggcg cacgcccacg cgcagcgcgg cctccagcag cgcgcgctgg
      421 cggcgggagt ggcgggcgtc gtactcctcc cacgacagcc gctcggccag ctccgcggcc
      481 acctcgacgg tcacggaggc cgacagcggc cgcccgccct ccacggcgct gcggatggcg
      541 ctgaccagct cctccaccgg cgcgcccttg gtcacgtagc ccacggcgcc cgcgcgcagc
      601 atctccagca ccgagatgcg gtcctcgtgc gcggacaggg ccaccacgcg cgtcagcggg
      661 ttgtgctcgc ggatgcgccg cgtcgcctcg gcgccgccgc cacccggcat ccgcacgtcc
      721 atgaggacca ggtcgggctc gtgggtctcg accagggtca cggcgccgtc ggggtcctgc
      781 gcgccgccca cgacctggaa gccgggttgg gcgtcgagca cctggcgcag cgcctcgtgg
      841 acgcgcccgt ggtcgtccac gacgccgata cggatgggcc tggtcatggc cgtcgcgctg
      901 ccactgcgtt cccccccgtc atggccggtc ggctcccgtg gtcggtccga tcatcgcgcg
      961 gccgtctccg ggaatcacgg cacaagccgt ctccacgcat cagaatccct ctcaacagcg
     1021 tgcgcagtac gcaattcgcg caagccgccc tacgcgaagc ggtctacgcg ggcgcacaga
     1081 tcccacttca cccgcactca ccttgaaagg actgggagtg ggcacggtta cattccgaag
     1141 caccgccgca cccgtcggcg ggctggggac ggggccgtga caagcaagac tggaaaggga
     1201 tcgggcgctt cgcgcgccgg gcgcgccagg ccggttcggc caccggagga gcgcgtgcgc
     1261 gtcctgctgg tggaagacca cgccgtggtc cgcgagggac tggcggccgt gctcgagcac
     1321 agcggcgcga tcagcatcgc gggccaggcc tccagcgtgc gcgacgggct gcgcatcctc
     1381 gagcgcgagg cggtcgacgt cgtggtgctg gacctgcggc tgggcgacga gcacggcgtc
     1441 gacctggtgc gcgaggtgcg ccgccgcgag gacggcccgg ccatcctggt gctcagcgcc
     1501 cacgacgcca ccctggaact ggagcgcgcg ctgggggccg gcgccaacgg ctacctgctg
     1561 aagggcagcg acgcggtgac ggtgacggac gcggtgctgg agacggccgc cgggcgcacc
     1621 gtgatcgaca cggggttcgt gcccaagctg ctgcaccgcg ccgcccacat ggaggaccag
     1681 ccgacgcccc gggagctgga ggtgctgcgg ctgtgcgccg aaggcgccac cgccgtggag
     1741 atcgcccggg agctgggcat ctcgccgcgc accgcgcaga agcacatcga gcggctgttc
     1801 aagaagctgg gcgtgtccaa ccgcgccgag ctggtgtcgg cggcgttccg ccgcgggctg
     1861 gtgcgctagg cgaggcggct ggcggcggtg gcgctgagcc aggcgccggc gaccgtgagc
     1921 agcacggcgc tggccagggg cagcgcgtcg agggcggcga ccaggcgcgg gctgccggca
     1981 aggcgggtga ccgcgaggtc gcggccgcgc agcaccgcca gccccaccag cgccaggctg
     2041 gccgccaggc ccatgctgaa tgccgccacc agcgccagcc cgaacagcgt ccggccggtg
     2101 aacagggccg tcgtcaggac caggaacgcc gagggcgagg gcagcaggcc gccggacatg
     2161 ccgatcagga ccagcccgcg gcgcgacagc ggcgacacgc cggccggcag cgccgcgtgg
     2221 tcgtgccacc ccggttcggc cgtggcgcgg cggtggcgca ggacgcgccc cagcgacgcg
     2281 gcgcccacgg ccagcacgag caggccggcg accagcgtca gccacggccc cgcccgctcc
     2341 agcgccgagc cgcccgccgc cagcgtgtgc agcgcgacgc cgagcaccag caccgacagc
     2401 gtgtgcatcg cggcgaccgc ggcgcccagc aggagcgcgt cgcggtcgcc gcgatgcaca
     2461 cgctgtcggt g
//
LOCUS       DATMKC010000421         6956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_73495, whole genome shotgun sequence.
ACCESSION   DATMKC010000421 DATMKC010000000
VERSION     DATMKC010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73495"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..1283)
                     /locus_tag="VNU01_12320"
     CDS             complement(420..1283)
                     /locus_tag="VNU01_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH33446.1"
                     /translation="MRAGDAPRLDGLADAVRETAALFSRVEAARDGWVNGQGGDVACA
                     GNHRALSRHTANYLAAAAMAGPAPAGARLVDVGGGVGAFTVWLAQRLGARPTLVDADP
                     DVLRVAAAAFPELSTAAGMGDTATGAAWLVTAMEVVEHVPPRAQLGFVRGLAGLVAPG
                     GALVVSTPDESRYMGRSSGYAPHVGVLDAAGLEGLLAEATGLPVQVWRLEGEAFHVGG
                     LERLVLPMANRAVGGLRRSVPGIMDAASHLWTRVGAARPRRAQPHVPAVRPVRASEGR
                     GSGLVAVVRFP"
     gene            1450..3660
                     /gene="tmk"
                     /locus_tag="VNU01_12325"
     CDS             1450..3660
                     /gene="tmk"
                     /locus_tag="VNU01_12325"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWH33447.1"
                     /translation="MDTGTSRGGHHTISAEPDPKLAHDAAGELVASGAQLGAYGALLR
                     SRDYRLWFASSLLSSLGDWIGLFALQVLILSTAPPGSRFALFGLGAIMMARLLPSVFF
                     GPVAGVLADRYDRRSLLVVTDLSRAVLFVFIATSREFATLFILTFAVECLSLLYSSAK
                     DASLPAIVNRRHLTQANQLNLFVAYGLMPFGAVVATAMVPASNLLQGLGVVAIDPTTL
                     ALLFNAGTFLLGGLLIWRVHLPHRRRDAEADRSEGVTEELRAGLRFIRGLPMVRALIT
                     GVVGVFFGAGVVVTLGPEFVRAELGRAEADWYGLMTMVGSGLLGGIALVPFLTARVRK
                     EKLFPVALGLAAALATLVAFLPTYALTQVVGLALGALAGMSIVIGYTMLHENTADDVR
                     AKTFAAFYTGTRASMFAALGLAPFLAGAIGTGTLILGARRIPISGVRVTILLGGLIAL
                     YSAVSSGRRIFASLRDDAEARPVRLRHGTPGAPLPGGLFVTFEGIEGSGKSTQIAALA
                     ASLAAEGYDVVVTREPGGPPIAERIRALLLDPNADGMHPRTEALLYAAARVEHVERVI
                     LPALEQGKVVLCDRYIDSSLAYQGFARELGDADIQEINRWATGGLLPDVVVLLRLDPE
                     EGLRRVADRSRTKAAEGDAGEGGEAGKLAYLAQRGPDRIERESVEFHRSVAEGYQALA
                     RRDRARYVVVDGAADPASVSRKVRAALHSWLPDPPSSAAPDVPEASADRPEAAG"
     gene            3657..4820
                     /locus_tag="VNU01_12330"
     CDS             3657..4820
                     /locus_tag="VNU01_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33448.1"
                     /translation="MSVWDLPGQATAADVLRRAVAAGEVSHAWAFVGPAGVGQQQAGR
                     ALAAALNCPVDPAGCGHCDVCGRVLRGAFPALWEFAPAGQAHRVADVRERWLPVASQT
                     AVEGRWKVLHIADADRMNEAAANAFLKGLEEPPERTIWLLDVADPDELPDTILSRCRV
                     VRFAPWSLEELRAHAARLGIPPEDHELAARLSQGAPRAMERLAGGGLEDVRRHRDLLR
                     RLREEGQGVALLAARELDEELKRRTAAQKAAGKDELADLAAAYGDALPRPVAKQVEER
                     LVREEREARASALQGALDDLLSWVRDALLLAGGGDPSAVLHCDAAGALRDDAAALGPA
                     GLLRAADLVQAAKQDLELNLQQGLALEALLLDLAALGMDVSRGAAAVAAHPGG"
     gene            4867..5835
                     /locus_tag="VNU01_12335"
     CDS             4867..5835
                     /locus_tag="VNU01_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWH33449.1"
                     /translation="MRRAVAALLVPALVLPGCRGPANEGTGPDGVRVVTTVAPIADLV
                     RRVAGGTAEVVALVPDGVDSHTFEPDPAQAADLAGARLFVANGLGLEEPSIRLAQANL
                     PEGASIVRLGDDTVPPEEWVFDRSFPEAGGVPNPHAWLDVPRAIRYVERIAQAVAGVV
                     AEDDRAQVRRNARALAAELTALHEAVETAVATVPARNRKLLTYHDSWAYFGRRYGFTV
                     VDAVQPAGMSEPSAAEVREVIDQIRAQGVPAVFGSEVFDDEVLRAIARETGVRFVGDL
                     ADDALPGEPGDPEHSYVGMMARNVRTVVEALGGDASALDRLSGVHQ"
     gene            5832..6644
                     /locus_tag="VNU01_12340"
     CDS             5832..6644
                     /locus_tag="VNU01_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWH33450.1"
                     /translation="MSAPALELAGVHAGYRGQVVLHDFGLRAEQGEFLGAVGPSGSGK
                     TTLLRLLTGRADLYAGRVRVLGQDVGRGRPPGRVGYVPQIGQVDWDFPLTVEQAVLLG
                     LTADSRWVPWFSRAERAAAAALLERLGLAGLQRRHIRDLSGGQQQRMFLARAMIRRCE
                     LVLLDEPTSGVDLRTRHDVLHLLHELNREGMTILLTTHDLNWVAAHLPRVVCLNGTVI
                     ADGPPTEVFTPEVLRRTYDADVRVIREGDLVFVADQTHLLTAAHHARGPDAP"
     gene            6641..>6956
                     /locus_tag="VNU01_12345"
     CDS             6641..>6956
                     /locus_tag="VNU01_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWH33451.1"
                     /translation="MNWLTEPLSYGFFARALVAGVALGAVTGAVGAFVVLRRMSYIGH
                     GLSHSLLGGIAVSLALGHGVYLGAAAATVVSALLIDRVSRRPGLAADAAIGIVTTAMF
                     ALG"
BASE COUNT          820 a         2576 c         2617 g          943 t
ORIGIN      
        1 accatgaaaa acacctccgg aaggtgtcag gaggaccacg ggcgtggcaa cgccccgacc
       61 ctcaacgaac ccttacgggt cccttctggc acctccggag gtgttcacca aggaaccgct
      121 ccgtgaccta tatcagccgg cgggcaggag cggcacccgg ctgaggatcg cctgcgcgag
      181 cgccgccgtc gagccgatca ttgcgacgat gagcgtgccg acgatgccga cggtccattt
      241 gtctgcctgc gcgcggcctt ctcgatgtgt gcgcgcagcc gctgctcaag gatctccagg
      301 tccgcctttg tcgcgaactg gtcctagtct tcgttcggca gcaggctcat gttcgcgagt
      361 gtgctgccga agcaggcagg cgtccaccgc tgggcgcccg aggttgtgga gaactcgggc
      421 taggggaagc ggacgacggc caccaggccg ctgcctcgtc cttccgatgc gcgcacgggc
      481 cgcaccgccg ggacgtgggg ctgcgcgcgg cgcggccgag ccgcccccac cctggtccac
      541 aggtggctcg cggcgtccat gatgcccggc acggagcgcc gcaggccgcc cacggcgcgg
      601 ttggccatcg gcagcaccag cctttcgagg ccgccgacgt ggaacgcctc gccctccagc
      661 cgccacacct gtaccggaag gcccgtggcc tcggccagca gcccttccag cccggctgcg
      721 tccaggacgc cgacgtgcgg cgcgtagccg gacgagcggc ccatgtatcg ggactcgtcc
      781 ggagtggaca ccaccagggc gccgcccggg gccaccaggc cggccagccc gcgcacgaag
      841 ccgagctgcg cccgcggcgg cacgtgctcg accacttcca tcgcggtgac cagccacgcc
      901 gcgccggtcg ccgtgtcgcc catgcccgcc gccgtcgaca gctcggggaa cgcggcggcg
      961 gccacgcgca gcacgtccgg gtccgcgtcg accagcgtgg gccgcgcccc cagccgctgc
     1021 gccagccata cggtgaacgc gcccaccccg ccaccgacgt ccaccagccg ggcgccggcc
     1081 ggcgcggggc ccgccatggc ggccgcggcc agatagttgg cggtgtggcg gctgagcgcg
     1141 cggtggttgc ccgcgcaggc gacatcgccg ccctggccgt tcacccagcc atcgcgcgcg
     1201 gcttcgacgc ggctgaacag cgcggcggtc tcgcgcacgg cgtcggccag gccgtcaagg
     1261 cgcggcgcgt caccggcgcg catggcgtca cgggtcgtgc tgatgggagt ccctccaggt
     1321 gctcgtgcgc gccggatgtg ccccggtggc accgatctca atcgccgccc cgggtggaac
     1381 ggcgaatgca aagctagcgg agcccccggc tacgctgtgc cgaccctgcg agcggcgtgt
     1441 gcccgagcgg tggacacggg cacgagccgg ggaggtcacc acaccatcag cgctgagccg
     1501 gacccgaagc tcgcccacga cgcggcgggc gagcttgtcg cgagcggcgc ccagctcggg
     1561 gcgtacggcg cgctgctgcg cagccgtgac taccgcctgt ggttcgcctc gtcgctgctg
     1621 tcgagcttgg gcgactggat cggcctgttc gcgctgcagg tgctgatcct gtcgacggct
     1681 ccgcccgggt cgcgcttcgc gctgttcggc ctgggcgcga tcatgatggc ccgcctgctg
     1741 cccagcgtgt tcttcgggcc ggtggccggc gtgctcgccg accgctacga ccgccgcagc
     1801 ctgctggtcg tcacggacct gtcgcgcgcg gtgctgtttg tcttcatcgc cacttcgcgc
     1861 gagttcgcga cgctgttcat cctcacgttc gcggtggagt gcctgtccct gctgtactcc
     1921 tcggccaagg acgcctcgct gccggccatc gtgaaccggc gccatctcac ccaggccaac
     1981 cagctcaacc tgttcgtggc ctacggcctc atgccgttcg gcgccgtggt ggccaccgcg
     2041 atggtccccg cgtcgaacct gctgcaaggc ctgggcgtgg tcgcgatcga ccccaccacc
     2101 ctggccctgc tgttcaacgc cggcacgttc ctgctgggcg gcctgctgat ctggcgcgtg
     2161 catctgccgc accgccggcg cgacgcggag gccgaccgct cggagggagt caccgaggag
     2221 ctgcgcgccg gcctgcgctt catccgcggc ctgcccatgg tgcgcgcgct catcaccggc
     2281 gtggtcgggg tgttcttcgg cgcgggtgtc gtggtgacgc tgggcccgga gttcgtgcgc
     2341 gccgagctcg gccgcgccga ggccgactgg tacggcctga tgaccatggt cggctccggc
     2401 ctgctgggcg gcatcgcgct ggtgccgttc ctgaccgcgc gcgtccgcaa ggagaagctg
     2461 ttccccgtcg ccctggggct cgcggccgcg ctggccacgc tggtggcgtt cctgccgacg
     2521 tacgcgctca cgcaggtggt cggcctggcg ctgggcgccc tggcgggcat gtccatcgtc
     2581 atcggctaca cgatgctgca cgagaacacg gccgacgacg tgcgcgccaa gacgttcgcg
     2641 gccttctaca ccggcacgcg cgcgtcgatg ttcgccgcgc tcggcctggc cccgttcctg
     2701 gccggcgcca tcggcaccgg cacgctcatc ctgggcgcgc gccgcattcc catctccggc
     2761 gtgcgcgtca cgatcctgct cggcggcctc atcgccctgt acagcgcggt cagctccggc
     2821 cgccggatct tcgccagcct gcgcgacgac gccgaggcgc ggccggtgcg cctgcgccac
     2881 ggcacgcctg gcgcgccgct gcccgggggc ctgttcgtga ccttcgaggg catcgagggc
     2941 tccggcaagt ccacgcagat cgcggccctg gcggcgtcgc tggccgccga gggctacgac
     3001 gtggtggtga cgcgcgagcc cggcggcccg cccatcgccg agcgcatccg tgccctgctg
     3061 ctggacccca acgccgacgg catgcatccg cgcaccgagg cgctgctgta cgccgcggcg
     3121 cgcgtggagc acgtcgagcg cgtgatcctg cccgccctcg agcagggcaa ggtcgtgctg
     3181 tgcgaccgct acatcgacag ctcgctggcc taccagggct tcgcacgcga gctcggcgac
     3241 gccgacatcc aggagatcaa ccgctgggcc acgggagggc tcctgccgga cgtggtggtg
     3301 ctgctgcggc tggaccccga ggagggcctg cgccgggtgg ccgaccgctc ccggacgaag
     3361 gcggccgagg gcgacgcggg cgagggcggc gaggccggca agctcgccta cctggcccag
     3421 cgcggcccgg accgcatcga gcgcgagagc gtcgagttcc accgctccgt ggcagagggc
     3481 taccaggcgc tggcgcgccg ggaccgcgcg cgctacgtcg tcgtggacgg cgcagccgac
     3541 cccgcctcgg tcagccgcaa ggtccgggcg gcgctgcact cgtggctgcc ggacccgccg
     3601 tcgtccgcgg ccccagacgt gccggaggcg tcggctgacc ggccggaggc cgcgggatga
     3661 gcgtctggga cctgccaggc caggccacgg cggccgacgt gctgcggcgc gccgtcgccg
     3721 cgggcgaggt cagccacgcg tgggcgttcg tgggcccggc cggggtcggc cagcagcagg
     3781 cgggccgtgc gctggccgcc gcgctgaact gccccgtcga ccccgccggc tgcggccact
     3841 gcgacgtgtg cggccgggtc ctgcgcggcg ccttccctgc gctgtgggag ttcgccccgg
     3901 ccggccaggc ccaccgcgtg gccgacgtgc gggagcgctg gctgccggtg gcgtcgcaga
     3961 cggcggtgga ggggcgctgg aaggtcctgc acatcgccga cgccgaccgc atgaacgagg
     4021 cggccgcgaa cgcgttcctc aaagggctgg aggagccgcc cgagcgcacg atctggctcc
     4081 tggacgtggc cgaccccgac gagctgccgg acacgatcct gtcgcgctgc cgggtggtgc
     4141 gcttcgcgcc gtggagcctg gaggagctgc gcgcgcacgc cgcgcgcctg ggcatcccgc
     4201 ctgaggacca cgagctcgcc gcccggctgt cgcagggcgc gccgcgcgcg atggagcgcc
     4261 tggcgggcgg cgggctggag gacgtgcgcc gccatcgcga cctgctgcgg cgtctgcgcg
     4321 aggaggggca gggcgtggcg ctgctggcgg cgcgggagct ggacgaggag ctgaagcggc
     4381 gcacggccgc gcagaaggct gcgggcaagg acgagctcgc ggacctcgcc gccgcctacg
     4441 gcgacgcgct gccgcgcccc gtcgccaagc aggtggagga gcgactggtg cgggaggagc
     4501 gcgaggcgcg cgcctccgcg ctgcagggcg ccctggacga cctgctgagc tgggtccgcg
     4561 acgcgctgct gctggccggc ggcggtgacc cgtcggcggt cctgcactgt gacgcggcgg
     4621 gggcgttgcg tgacgacgcg gccgcgctcg ggcccgccgg gctgctgcgc gcggccgacc
     4681 tggtgcaggc cgcgaagcag gacttggagc tcaacctcca gcaaggcctg gcgcttgagg
     4741 cgctgctgct ggacctcgcg gcactcggca tggacgtgtc gcgcggcgcg gcggcggttg
     4801 ccgcgcaccc gggcggctga ctacgctagt tgcaaatcgt ttgcaactag ctccggagtc
     4861 ccatccatgc gccgcgcggt cgccgccctg ctcgtcccgg ccctcgtgct gcccggctgc
     4921 cgcggcccgg cgaacgaggg caccggcccg gacggcgtgc gcgtcgtgac cacggtcgcg
     4981 ccgatcgccg acctggtccg cagggtggcc ggcggcaccg cggaggtcgt ggcgctggtg
     5041 ccggacggcg tcgactcgca cacgttcgag ccggacccgg cgcaggccgc ggacctggcc
     5101 ggcgcccgcc tgttcgtcgc gaacgggctg gggctggagg agccgtccat ccgcctggcg
     5161 caggcgaacc tgccggaggg cgcgtccatc gtgcgcctgg gcgacgacac cgtgccgccg
     5221 gaggagtggg tgttcgaccg ctccttcccc gaggccggcg gggtgcccaa cccccacgcg
     5281 tggctcgacg tcccgcgcgc catccgctac gtggagcgca tcgcgcaggc cgtcgccggc
     5341 gtggtcgcgg aggacgaccg cgcgcaggtg cggcgcaacg cgcgcgcgct cgccgcggag
     5401 ctcaccgcac tgcacgaggc cgtggagacc gccgtggcca cggtgcccgc gcgcaatcgc
     5461 aagctgctca cctatcacga ctcctgggcc tacttcggcc ggcgctacgg cttcacggtc
     5521 gtcgacgcgg tgcagcccgc gggcatgagc gagccgtccg cggcggaggt gcgcgaggtg
     5581 atcgaccaga tccgcgcgca gggcgtgccg gcggtgttcg gcagcgaggt gttcgacgac
     5641 gaggtgctgc gcgcgatcgc gcgggagacc ggggtgcgct tcgtgggcga cctggccgac
     5701 gacgccctgc ccggcgagcc gggcgacccc gagcactcct acgtggggat gatggcccgc
     5761 aacgtgcgca ccgtggtgga ggcgctgggc ggcgacgcgt ccgcgctgga ccgcctttcg
     5821 ggggtgcacc agtgagcgcg ccggcgctgg agctggccgg tgtccacgcc ggctaccgcg
     5881 ggcaggtggt gctgcacgac ttcggcctgc gtgccgagca gggtgagttc ctgggcgcgg
     5941 tgggcccgag cggctcgggc aagaccacgc tgctgcggct gctgaccggc cgcgccgacc
     6001 tctacgccgg gcgggtccgc gtgctcggcc aggacgtcgg ccgcggccgc ccgcccggcc
     6061 gcgtcggcta cgtgccgcag atcgggcagg tggactggga cttcccgctg acggtggagc
     6121 aggcggtgct gctgggcctc accgccgaca gcagatgggt gccgtggttc agcagggcgg
     6181 agcgcgccgc cgccgccgcg ctgctggagc gccttgggct ggccgggctg cagcgacgcc
     6241 acatccgcga cctgtccggc ggccagcagc agcgcatgtt cctggcgcgg gcgatgatcc
     6301 gccgctgcga gctggtgctg ctcgacgagc cgaccagcgg ggtggacctg cgcacgcgcc
     6361 acgacgtgct gcacctgctg cacgagctca accgcgaggg catgacgatc ctgctcacca
     6421 cccacgacct gaactgggtg gccgcgcacc tgccgcgcgt ggtgtgcctg aacggcacgg
     6481 tgatcgcgga cggccccccc acggaggtgt tcacgccgga ggtgctgcgg cgcacctacg
     6541 acgccgacgt gcgcgtcatc cgcgagggcg acctggtgtt cgtggccgac cagacgcacc
     6601 tgctcaccgc ggcgcaccac gcgcgcggcc cggacgcgcc gtgaactggc tgaccgagcc
     6661 gctgtcgtac ggcttcttcg cgcgcgcgct cgtggccggc gtcgcgctcg gcgccgtgac
     6721 gggcgccgtg ggcgcgttcg tggtgctgcg ccgcatgagc tacatcggcc acgggctgtc
     6781 ccacagcctg ctcggcggca tcgccgtgtc gctggcgctg ggccacggcg tctacctggg
     6841 cgccgccgcg gccaccgtgg tgagcgccct gctgatcgac cgcgtgtcgc ggcggcccgg
     6901 actggccgcc gacgccgcga tcggcatcgt caccacggcg atgttcgccc tgggca
//
LOCUS       DATMKC010000422         5055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_321788, whole genome shotgun sequence.
ACCESSION   DATMKC010000422 DATMKC010000000
VERSION     DATMKC010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321788"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VNU01_12350"
     CDS             complement(<1..355)
                     /locus_tag="VNU01_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33452.1"
                     /translation="MTDTAPASTGPPGPRPKVALVTLGCGRNEVDSEQVGGSLLAAGY
                     DVVDDPEQADCVLVNTCAFIEPAREESVETVLAAADLPGAKVVVMGCMAQRYGDELAG
                     AIPEAAAVVGFADYRR"
     gene            complement(413..973)
                     /locus_tag="VNU01_12355"
     CDS             complement(413..973)
                     /locus_tag="VNU01_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH33453.1"
                     /translation="MTTGIGEALRAARRQQGRSLADAAAATRVRESYLAALEEEEFAA
                     LGGDVYVKGFLRSYARFLGLNPEPLLESYRREHERPDEPQPLTASAGRSFDPDARRGL
                     SPLAVAGTLLFTILALYLVFSRGNEEPATVPAPAPVESSEGIPGGGAEPDAGGPAVPD
                     TQTMDPYAPAVEPTVDDGAGLDADAG"
     gene            complement(1000..1842)
                     /locus_tag="VNU01_12360"
     CDS             complement(1000..1842)
                     /locus_tag="VNU01_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HWH33454.1"
                     /translation="MRKTAGLDAVAAQRARTVALAEGLTAEDWDLPCLPRWAVRDVFA
                     HLVASDRASITGAIVRPLLGTSDRAVVERWNDQAVRRWRDRPPQDIVDGLRRWGARIE
                     RLGRLVPGPVARLPVGTPYGRHPVVFLLFLRVWDEWVHEQDVRWALGAPRDDVPSPPP
                     LVIGQALADMTLAVLPRRRLPEVARRTGIARLVVDPAPPDGAVTARPVTWGVDFARRQ
                     YGPRVTAKADAEVRVHAAALALLLGGRMHWRALRAPWLSVDGDGELAAALLDAVPLPS
                     SDPV"
     gene            complement(1870..2772)
                     /locus_tag="VNU01_12365"
     CDS             complement(1870..2772)
                     /locus_tag="VNU01_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH33455.1"
                     /translation="MSQTVVVRRGGAPDPQPEPEAAIRIRNLTKSFGSRTVLEDITFD
                     IPKGEVTVVLGPSGTGKSVFLRHLIGLLRPDSGEVWVGDRNVPTLRQRELLEVRKKFG
                     VLFQDGALFGSMTVYDNTAFPLREHTRKSEREIEEIVMQKLTMVGLADARNLFPGEIS
                     GGMRKRAGLARALVLDPEIVLFDEPDSGLDPVRTAYLNELILDLKEQLDSTFVVVTHH
                     IPTARNIADYLALLFRRNLVMMGPKQELLDSDKPVVRQFLRGSTRGPIGMSEEKDASD
                     PNATYEEGGLSLEDTRDEEDLPGR"
     gene            complement(2806..3648)
                     /locus_tag="VNU01_12370"
     CDS             complement(2806..3648)
                     /locus_tag="VNU01_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWH33456.1"
                     /translation="MSTYPAVIVGTDGSATAAEAVRRAALVAGRFGARLLVVTAFQRP
                     RPEDMGPASELAGQSQEALISTSYRGAAEAAQDAATMAARLVPGLSVDTATPEGEPAE
                     QLLEMAEVNPGALLTVGSQGMTGSKRFLLGSVPNKVSHHLVGDLLIFRTGVGRPVEPP
                     SSVLVGTDGSATATRALDRAIAVARDLGATLTVLTVNGDAEQGRRVLDEAASRAEQAG
                     VTCQTALRTGDPAEAIVAEGQSADLVVLGSQGMTGAGRFLLGSVPNKVSHHASSDLLI
                     VKTS"
     gene            complement(3645..4229)
                     /locus_tag="VNU01_12375"
     CDS             complement(3645..4229)
                     /locus_tag="VNU01_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33457.1"
                     /translation="MTDETAAPAAAAKPPLVRRLGFAWSVAAVTTVIALLFAVMWAPL
                     YSQDRARDRVRAAAEQLVLHLTTFEGATIDDWVQETQRRATDEYAEEVNTLFDPELRA
                     ALRDAEAKSVGRLVNLFVQDVDGDDATVFAVVRQTITNARAPQPVEDELRMQISLVRE
                     GGTWKAARVEVLGPQESGQAPAPGGGLLEEGDGS"
     gene            complement(4226..>5055)
                     /locus_tag="VNU01_12380"
     CDS             complement(4226..>5055)
                     /locus_tag="VNU01_12380"
                     /inference="COORDINATES: protein motif:HMM:NF037542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HWH33458.1"
                     /translation="GERAGVLVAELADAVRGRRDDLRAIIADSARLSEALAANEADVT
                     RFFTHSATVNEALARSRDALARSIAQMADASRTLVDMRADFEGLLREAPPVLRQVEKL
                     VGDNQANVSCVFKDLGNLNSYLARPEQMGNTEEALRKNQWFFQGVESGGPLDPWGRVW
                     TRIKFLSPQAEQPDSYLPDKRPIPDILPGGTCRSPLGTGAGAATQPGFERTTIEARIV
                     PAEQDANASGLGLAAPAASAASSDSRRVAESLGLLLALAASMVAGRAVRRVIAKGNR"
BASE COUNT          628 a         1947 c         1806 g          674 t
ORIGIN      
        1 gccggcggta gtcggcgaag ccgacgacgg cggccgcctc ggggatcgcc ccggccagct
       61 cgtcgccgta gcgctgcgcc atacagccca tcaccacgac cttggcgccc ggcagatccg
      121 ccgccgcaag cacggtctcc accgactcct cgcgcgccgg ctcgatgaac gcgcacgtgt
      181 tcaccagcac gcagtcggcc tgctcgggat cgtcgaccac gtcgtagccg gcggccagca
      241 gcgagccgcc gacctgctcg ctgtccacct cgttgcggcc gcagcccaag gtcaccaggg
      301 cgaccttcgg gcgcggccca ggggggccgg tggaggcggg agcggtgtcg gtcacgcggt
      361 cacgatgcca cggcgcggca cgcgcgcacc aacgccgccg gcccgcgctg gctcagccgg
      421 catccgcgtc gagccccgcg ccgtcgtcca ccgtcggctc cacggcgggg gcgtaggggt
      481 ccatcgtctg ggtgtccggc acggccggcc cgcccgcgtc cggttcggct ccgccgccgg
      541 gtatgccctc gctgctctcc acaggggccg gcgccggcac ggtcgccggc tcctcgttgc
      601 cgcggctgaa caccaggtac agcgccagga tcgtgaacag cagcgtgccg gccaccgcca
      661 gcggcgacag cccgcggcgc gcgtccgggt cgaacgaccg gccggccgag gcggtcagcg
      721 gctgcggctc gtcgggacgc tcgtgctcgc ggcggtagga ctccagcagc ggctccgggt
      781 tcagccccag gaagcgggca tagctgcgca ggaagccctt gacgtagacg tccccgccga
      841 gcgcggcgaa ctcctcctcc tccagcgcgg cgagatagga ctcccgcacg cgcgtggcgg
      901 ccgccgcgtc ggcgaggctg cgcccctgct ggcggcgcgc tgcgcgcagt gcctcaccga
      961 ttcccgtcgt catcgtgggg cgagtatagg agcgcgtgct cagaccgggt cggaggacgg
     1021 cagcggcacc gcgtccagca gcgcggcggc cagctcgccg tcgccgtcga cgctgagcca
     1081 cggcgcgcgc agcgcccgcc agtgcatgcg gccgcccagc agcagcgcca gcgcggcggc
     1141 gtgcacgcgg acctccgcgt cggctttggc ggtcacgcgc ggcccgtact ggcgccgcgc
     1201 gaagtcgacg ccccacgtga cgggccgcgc cgtcacggcg ccatccggcg gggccgggtc
     1261 caccaccagc cgcgcgatcc cggttcgccg ggcgacctcc ggcaggcggc gcctgggcag
     1321 caccgccagc gtcatgtcgg ccagcgcctg cccgatcacc agcggcggcg gcgacggcac
     1381 gtcgtcacgg ggcgcgccga gcgcccagcg cacgtcctgc tcgtgcaccc actcgtccca
     1441 gacccgcagg aacagcagga acaccacggg atggcgcccg tacggcgtgc cgacgggcag
     1501 gcgcgccacc ggcccgggca ccaggcggcc caggcgctcg atgcgggcgc cccagcggcg
     1561 cagcccgtcg acgatgtcct gaggcgggcg gtcgcgccag cgccgcaccg cctggtcgtt
     1621 ccagcgctcc acgacggcgc ggtcggaggt ccccagcagc ggccggacga tggcgccggt
     1681 gatggacgcg cggtcgctgg ccaccaggtg cgcgaacacg tcccgcaccg cccagcgcgg
     1741 caggcacggc agatcccagt cctcggccgt cagcccctcg gccagcgcca ccgtgcgtgc
     1801 ccgctgtgcc gcgaccgcgt ccaaccctgc tgtcttgcgc atgcccgctc ccggcacgct
     1861 gcccgggcgt cagcgcccgg gcaggtcttc ctcgtcgcgc gtgtcctcca gcgacagccc
     1921 gccctcctcg taggtggcgt tggggtcgct ggcgtccttc tcctccgaca tgccgatggg
     1981 cccgcgggtg gagccgcgca ggaactggcg caccaccggc ttgtcggagt ccagcagctc
     2041 ctgcttgggg cccatcatca ccaggttgcg gcggaacagc agcgccaggt agtcggcgat
     2101 gttgcgggcc gtggggatgt ggtgggtcac caccacgaac gtcgagtcga gctgctcctt
     2161 caagtccagg atcagctcgt tgaggtaggc ggtgcgcacc gggtccaggc cggagtcggg
     2221 ctcgtcgaac agcacgatct cggggtcgag caccagcgcg cgcgccaggc cggcgcgctt
     2281 gcgcatgccg ccggagatct ccccggggaa caggttgcgc gcgtccgcca ggccgaccat
     2341 cgtcagcttc tgcatgacga tctcttcgat ctcgcgctcg ctcttgcggg tgtgctcgcg
     2401 cagcgggaag gcggtgttgt cgtacaccgt catggagccg aacagcgcgc cgtcctggaa
     2461 cagcacgccg aacttcttgc gcacctccag cagctcgcgc tggcgcagcg tcggcacgtt
     2521 gcggtcgccg acccagacct cgccgctgtc ggggcgcagc aggccgatga ggtggcgcag
     2581 gaacaccgac ttgcccgtgc cggacgggcc cagcaccacg gtgacctccc ccttggggat
     2641 gtcgaacgtg atgtcctcca gcaccgtgcg ggagccgaac gacttggtca ggttgcggat
     2701 gcggatggcc gcctccggct ccggctgcgg atccggcgcc cctcccctgc ggaccacgac
     2761 ggtctggctc atagcggcac tcctacataa gtggacaggc tgggcctacg acgtcttcac
     2821 gatgagcagg tcgctggacg catggtggct gactttgttc ggcacgctgc ccagcaggaa
     2881 gcggcccgcc ccggtcatgc cctggctgcc caggaccacc aggtccgcgg actggccctc
     2941 cgccacgatg gcctcggcgg ggtcgccggt tcgcagcgcg gtctggcacg tcacaccggc
     3001 ctgctcggcg cgcgacgcgg cctcgtccag gacacgccgg ccctgctcgg cgtcgccgtt
     3061 cacggtcagc acggtgagcg tcgcgccgag gtcgcgcgcc accgcgatcg cgcggtccag
     3121 cgcgcgcgtc gccgtggccg agccgtccgt gccgacgagc accgacgacg gcggttccac
     3181 cgggcgtccc acgcccgtgc ggaagatcag caggtcgccg accaggtggt ggctgacctt
     3241 gttcgggacg ctgccgagca ggaagcgctt ggacccggtc atgccctgcg agcccacggt
     3301 cagcagcgcc ccgggattga cctcggccat ctccagcagc tgctcggccg gctcgccctc
     3361 gggcgtggcg gtgtcgaccg acaggccggg caccaggcgc gccgccatgg tggccgcgtc
     3421 ctgcgcggcc tcggctgcgc cgcggtagct ggtgctgatc agcgcctcct ggctctggcc
     3481 ggccagctcc gaggcggggc ccatgtcctc cggccggggc cgctggaagg cggtcaccac
     3541 cagcaggcgc gcgccgaagc gcccggcgac gagcgcggcg cgccgcaccg cctcggccgc
     3601 ggtggccgag ccgtccgtgc cgacgatgac ggcggggtac gtgctcacga tccgtctccc
     3661 tcctccaaca ggccgccgcc cggcgccggc gcctggccgc tttcttgcgg tcccagcacc
     3721 tccacgcgcg cggccttcca cgtgccgccc tcccggacca gcgagatctg catgcgcagc
     3781 tcgtcctcca ccggctgcgg ggcgcgcgcg ttggtgatcg tctggcgcac cacggcgaac
     3841 accgtcgcgt cgtcgccgtc gacgtcctgg acgaacaggt tcaccagccg gccgacggac
     3901 ttcgcctccg cgtcgcgcag ggcggcgcgc agctccgggt cgaacagcgt gttgacctcc
     3961 tcggcgtact cgtcggtggc gcgacgctgc gtctcctgca cccagtcgtc gatggtcgcg
     4021 ccctcgaacg tggtcaggtg caggaccagc tgctccgcgg ccgcgcgcac gcggtcgcgg
     4081 gcacggtcct ggctgtacag cggcgcccac atcacggcga acagcagcgc gatcaccgtc
     4141 gtgacggccg cgacggacca ggcgaagccc aggcgccgca ccagcggcgg cttggcggcg
     4201 gccgccgggg ccgcggtctc gtcggtcatc ggttgccctt cgcgatcacg cgccggaccg
     4261 cgcgcccggc gaccatgctg gccgccagcg ccagcagcag gcccagcgac tcggcgacgc
     4321 ggcggctgtc gctactggcc gccgacgccg ctggcgccgc caggcccagg ccggacgcgt
     4381 tcgcgtcctg ctctgccggc acgatccgcg cctcgatggt ggtgcgctcg aagccgggct
     4441 gggtggccgc gcccgccccg gtgcccagcg gcgagcggca cgtcccgccc ggcaggatgt
     4501 cggggatcgg gcgcttgtcc ggcaggtagc tgtccggctg ctcggcctgc ggcgacagga
     4561 acttgatgcg cgtccacacg cggccccacg ggtccagcgg cccgccgctc tccacgccct
     4621 ggaagaacca ctggttcttg cgcagcgcct cctcggtgtt gcccatctgc tccggccgcg
     4681 ccaggtagga gttgaggttg cccaggtcct tgaacacgca ggagacgttc gcctggttgt
     4741 cgccgaccag cttctcgacc tggcgcagca ccggcggcgc ctcgcgcagc agcccttcga
     4801 agtcggcgcg catgtccacc agcgtgcggc tcgcgtcggc catctgggcg atggagcgcg
     4861 cgagcgcgtc ccggctgcgc gccagcgcct cgttcaccgt ggcgctgtgc gtgaagaacc
     4921 gggtgacgtc ggcctcgttg gccgccagcg cctccgacag ccgcgcgctg tcggcgatga
     4981 tcgcgcgcag gtcgtcgcgg cggccgcgca ccgcgtcggc cagttccgcg accagcacgc
     5041 cggcgcgctc cccgt
//
LOCUS       DATMKC010000423         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_16828, whole genome shotgun sequence.
ACCESSION   DATMKC010000423 DATMKC010000000
VERSION     DATMKC010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16828"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..700)
                     /locus_tag="VNU01_12385"
     CDS             complement(38..700)
                     /locus_tag="VNU01_12385"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33459.1"
                     /translation="MDLELIRTGLQNAWENTAAATPKVVGALAIFLVGWLLAGVVRMV
                     AHRVMDLIRFDQIVERAGVPAASERSGYDPKGLVAGIAYWTVLLLTVQLAADTLELTA
                     LSAALAGMVAYLPQVLVAVGVVVVAMAFGNFVAGAVHSNTRSELATHVARYSIYGFGA
                     FAALNQLGIAEDIVNALFYATVATIGATCVVAFGVGGIPAARGMTERWFGRKDDRGVR
                     AA"
     gene            931..1494
                     /locus_tag="VNU01_12390"
     CDS             931..1494
                     /locus_tag="VNU01_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWH33460.1"
                     /translation="MLRARDRGDGAALEELIRRHRDAAYRIALRICLNGADAEDVAQE
                     AFVRVWRSLGRFRADSAFSTWLYRIVTNLALNTVTRRREQPAELAESQFPAELDPASR
                     VQQRERLAALGAALASLTPEQRACWVLREVEGLSYEELAEVLELTLASVKGRLFRARA
                     ELAAALARYDAPEGDAEAEPDAEGGGR"
     gene            1491..1730
                     /locus_tag="VNU01_12395"
     CDS             1491..1730
                     /locus_tag="VNU01_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33461.1"
                     /translation="MSERDDWVDEDPGDPALHAELASLLRAAAPPAPAVVERRALGRI
                     RVERDMLTVASTLGGALLRVLGALPDYLAAERRRH"
     gene            1730..2518
                     /locus_tag="VNU01_12400"
     CDS             1730..2518
                     /locus_tag="VNU01_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33462.1"
                     /translation="MDLDGLRWEGPLLAAVGFALLGVAAALVVNLLVRAIGRRVLRDR
                     DRVDGMAAFSGWIVLAVFLVVAIGRLADPDITEPGLRAAAARSLGLLPDVLIAVLLVA
                     FGVVLATALRGVLRRLLEPVRPAAAEMVPSVAYWAVVGLALLLGADQIGVETGLVVWL
                     LVTLVGGVVLAFALALGLGAKDLLGAVVAGRHVAQIVAVGDEVEVDGKRGTVVALGHA
                     SVRLSLAGTRGVGLSAGESEGVEAEVPNRRFLEGTVLVHRRAGG"
BASE COUNT          326 a          948 c         1063 g          379 t
ORIGIN      
        1 cgacgtggtg tcgcgacggc cttcggtgtt cgcgggccta tgcggcgcgc acgccccggt
       61 cgtccttgcg gccgaaccag cgctccgtca tgccgcgggc ggcggggatc ccgccgacgc
      121 cgaacgccac cacgcaggtc gcgccgatgg tggcgacggt ggcgtagaac agcgcgttca
      181 cgatgtcctc ggcgatgccg agctggttga gcgccgcgaa cgcgccgaag ccgtagatcg
      241 agtagcgggc gacgtgcgtc gccagctccg agcgggtgtt ggagtgcacg gcgccggcca
      301 cgaagttgcc gaacgccatg gcgacgacca ccacaccgac ggccacgagc acctgcggca
      361 ggtaggccac catgccggcc agcgccgccg acagggcggt cagctccagc gtgtcggccg
      421 ccagctgcac ggtgagcagc agcacggtcc agtaggcgat gccggcgacg aggcccttcg
      481 ggtcgtagcc ggagcgctcc gaggcggcgg gcacgcccgc gcgctcgacg atctggtcga
      541 agcggatcag gtccatgacg cggtgcgcga ccatgcgcac gacgccggcg agcagccagc
      601 ccaccaggaa gatcgccagc gcgccgacca ccttgggggt cgccgcggcc gtgttctccc
      661 aggcgttctg caggccggtg cggatgagct cgaggtccat gggggaagca cctccgtcgc
      721 cgcaatggat ctcgcacggc cccccggtgc ggcaccggga ccgtggcggg cgtgtcgccc
      781 ttcacccgtc agacccagcc gccggattaa agttccgtgg tcggaagaaa ctttcttcga
      841 cggtcatgga ggctcgccgc gcacgccgca cagccgcacc cgcagccgca ttctcagggg
      901 ccgctggggc gcctcagcga cgccgagctg gtgctgcgcg cgcgcgaccg cggtgacggc
      961 gcggcgctgg aggagctgat ccggcgccac cgcgacgcgg cctaccgcat cgccctgcgc
     1021 atctgcctga acggcgccga cgcggaggac gtcgcccagg aggcgttcgt gcgcgtctgg
     1081 cgctcgctcg gccggttccg ggccgacagc gccttctcca cctggctgta ccgcatcgtg
     1141 acgaacctgg ctttgaacac ggtgacccgg cggcgcgagc agccggccga gctcgccgaa
     1201 tcgcagttcc ccgcggaact cgaccccgcc agccgggtcc aacagcgcga gcgcctcgcc
     1261 gccctcggcg cggcgctggc gtccctgacg cccgagcagc gcgcctgctg ggtgctgcgc
     1321 gaggtggagg ggctgagcta cgaggagctg gccgaggtgc tggagctgac tctggcgtcg
     1381 gtgaagggcc ggctgttccg ggcgcgcgcg gagctcgcgg cggccctggc acgctacgac
     1441 gccccagagg gtgatgcaga ggcggaaccg gacgcggaag gaggtgggcg gtgagcgagc
     1501 gcgacgactg ggtcgacgag gacccgggcg acccggcgct gcacgccgag ctcgcctcgc
     1561 tcctgcgcgc cgccgcgccg cccgcgcccg cggtggtgga gcgccgcgcc ctgggccgca
     1621 tccgcgtgga gcgcgacatg ctcaccgtgg cgtcgacgct cggcggcgcg ctgctgcgcg
     1681 tgctgggcgc cttgccggac taccttgctg ccgagcggag gagacactga tggacctcga
     1741 cgggctgcgc tgggaggggc cgctgctggc ggcggtgggc ttcgcgctgc tcggcgtggc
     1801 ggccgcgctg gtcgtgaacc tgctggtgcg cgccatcggc aggcgcgtgc tgcgcgaccg
     1861 tgaccgggtc gacggcatgg cggcgttctc cggctggatc gtgctggccg tgttcctggt
     1921 ggtggccatc ggccgcctgg ccgaccccga catcaccgag ccgggcctgc gcgccgcggc
     1981 ggcgcgctcc ctgggcctgc tgccggacgt gctcatcgcg gtgctgctgg tggccttcgg
     2041 ggtggtcctg gcgaccgcgc tgcgcggcgt gctgcgccgc ctgctggagc cggtgcgtcc
     2101 ggcggcggcg gagatggtgc ccagcgtcgc ctactgggcg gtggtgggcc tggcgctgct
     2161 gctcggcgcc gaccagatcg gcgtggagac cgggctggtg gtgtggctgc tggtcacgct
     2221 ggtggggggc gtggtgctgg cgttcgcgct ggcgctgggc ctcggggcca aggacctgct
     2281 cggcgccgtc gtggcggggc gccacgtcgc gcagatcgtc gccgtgggcg acgaggtcga
     2341 ggtcgacggc aagcggggga ccgtcgtggc gctgggccat gccagcgtcc ggctgtcgct
     2401 cgcgggcacg cgcggggtcg gcctgtccgc gggggagtcc gagggcgtgg aggccgaggt
     2461 ccccaaccgc cgcttcctgg aggggaccgt cctggtccac cgccgcgccg gaggctgagc
     2521 gccgggtcgc gccggatgga cacctgtgcg cacgggggaa cacctgtacg gtgggacgac
     2581 ccgccccagg ttcaccgacg ccttcgaggg ggcagatcct cttcgtcggc cggtttcatc
     2641 gagcacatcg gatggggcat atgcggatcc ttcaactggc tccgccatgg ttcgcggtcc
     2701 cgccgtccgg ttacgg
//
LOCUS       DATMKC010000424         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_9511, whole genome shotgun sequence.
ACCESSION   DATMKC010000424 DATMKC010000000
VERSION     DATMKC010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9511"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VNU01_12405"
     CDS             <1..1100
                     /locus_tag="VNU01_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWH33463.1"
                     /translation="HHAVAAIVALLRRHGHEAEVGAYGLPTALRARAGSGRPTVALLA
                     EYDALPDIGHACGHNVIAATAVGAFLAVAELIGELGGSVELIGTPAEEGGGGKELIAQ
                     AGGFDAVDAALMLHPFGADAAEHPWLGVRQVEAVFHGLPAHASAMPFFGRNALDAAVT
                     AYQSMAQLRQHMLPTDRVHGIFTDGGQKPNIVPERAAVQFYLRSAEPDTLRVLSERAG
                     QIFEGAALATGTRLELRWDTAPVYLPVRNNHALAARYAANLAPRGRKVLPIGVLPGEI
                     SGSTDMGNVSVRVPGIHPLLAIAPPYVTIHTAPFAEHAVSEAADAGCVDGAVGLALTA
                     ADFLTDEALRTDARAEFDAAGGVIEPDSLLT"
     gene            1215..1445
                     /locus_tag="VNU01_12410"
     CDS             1215..1445
                     /locus_tag="VNU01_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33464.1"
                     /translation="MGDIDTKRIEDVEALRARIRRWQDLYEERDAIGYDPKRHDAHSP
                     EHRRYTRITWDIHRIEDELFANWDTMTTTSAR"
     gene            complement(1554..2270)
                     /locus_tag="VNU01_12415"
     CDS             complement(1554..2270)
                     /locus_tag="VNU01_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33465.1"
                     /translation="MSSLLCAFLRACVAAVPRAGRGPAAVWASPGPAAVGSVRLGQLA
                     AGVLLIGTGVGAMLSSGLGVGAWDAFHVGVSGAFGVPLWAAIAGTGAVTLAAGVALGG
                     RPRWGTFAPLCVGPVVQATMGLLPAPGNPLQAWLLLAVGVGVVATGAGAYLTAGCGVG
                     PFDLVFHGLNRRGLPLTAARLCVDGAALVVGALLGGPVGAGTVAATVALGLLIPATVR
                     RFERYAPAPRLSARRVLVAA"
     gene            complement(2461..3087)
                     /locus_tag="VNU01_12420"
     CDS             complement(2461..3087)
                     /locus_tag="VNU01_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HWH33466.1"
                     /translation="MSDEQASGVTGPVVGVGPWEGPWPDDPRLDPDLLAHGDRRNVTD
                     RYRYWTVEAITADLDTRRHPFHVAIENWTHDFNIGTVVRNANAFNAASVRIVGRRKWN
                     RRGAMMTETYQHVVHHDTVGDLVAWAQDAGLPLVGVDNLPGAVPLAQADLPRACVLLF
                     GQEGPGLSQAAREAAAVVVSIPQFGSTRSINAGVASGVAMHEWVRRHA"
BASE COUNT          504 a         1260 c         1192 g          359 t
ORIGIN      
        1 agcaccacgc cgtcgcggcg atcgtggcgc tgctgcgccg tcacgggcac gaggcggagg
       61 tcggcgccta cgggttgccc acggcgctgc gagcacgggc cgggagcggc aggcccacgg
      121 tggcgctgct ggccgaatac gacgcgctgc ccgacatcgg ccacgcctgc ggccacaacg
      181 tgatcgcggc cacggcggtc ggcgcgttcc tggcggtcgc ggagctgatc ggcgagctgg
      241 gcggcagcgt ggaactcatc ggcacgcccg cggaggaggg cggcggcggc aaggagctga
      301 tcgcccaggc cggcggcttc gacgcggtgg acgcggcgct gatgctgcac ccgttcgggg
      361 ccgacgcggc cgagcacccg tggctgggcg tgcgccaggt cgaagcggtg ttccacggcc
      421 tgcccgcgca cgcctccgcg atgccgttct tcggccgcaa cgccctggac gccgcggtga
      481 ccgcgtacca gtcgatggcg caactgcgcc agcacatgct gccgaccgac cgcgtccacg
      541 gcatcttcac cgacggcggc cagaagccga acatcgtgcc ggagcgcgcc gccgtgcagt
      601 tctacctgcg ctcggcggag ccggacacgc tgcgcgtgct gtcagagcgc gccggtcaga
      661 tcttcgaagg cgcggcgctg gccaccggca cgcgcctgga gctgcgctgg gacaccgccc
      721 cggtgtacct gccggtgcgc aacaaccacg cgctggccgc ccgctacgcc gccaacctgg
      781 cgccgcgcgg ccgcaaggtg ctgcccatcg gcgtgctgcc cggcgagatc agcggctcca
      841 cggacatggg caacgtgagc gtgcgggtgc ccggcatcca cccgctgctg gcgatcgcgc
      901 cgccctacgt caccatccac accgcgccgt tcgccgagca cgccgtcagc gaggcggccg
      961 acgccggctg cgtcgacggg gccgtcggcc tggcgctgac cgccgcggac ttcctcaccg
     1021 acgaggcgct gcgcaccgac gcccgcgccg agttcgacgc cgccggcggc gtgatcgagc
     1081 cggacagcct gctgacctga cgggggccca caccggtccg cggctaccgc gcaggaagca
     1141 ctacagattc cggccgcgac agccgacatg gttggcacgc cactccccaa gtgcgcgagg
     1201 accggagcag cgacatgggt gacatcgaca ccaagcggat cgaggacgtg gaggcgctgc
     1261 gcgcccgcat ccgccgctgg caggacctct acgaagagcg cgacgccatc ggctacgacc
     1321 ccaagcgtca cgacgcgcac agcccggagc accgccgcta cacgcgcatc acctgggaca
     1381 tccaccgcat cgaggacgag ctgttcgcca actgggacac catgaccacc acatcggcaa
     1441 ggtgacgggg ccgggctcca gcgctccgga gcccgcctcc cgttccttcc cggaaccccc
     1501 atccgccccc gtgaccccac cacacggggg cggatccctg tccgcggccg gggtcaggcc
     1561 gcgaccagca cccgccgcgc ggacaggcgc ggcgccggcg cgtagcgctc gaagcgccgc
     1621 accgtggccg ggatcagcaa gccgagcgcc actgtcgccg cgaccgttcc ggcgcccacc
     1681 gggccgccca ggagcgcacc caccaccagc gcggcgccgt cgacgcacag gcgcgccgcc
     1741 gtcaggggca gcccgcgccg gttcaggccg tggaagacca ggtcgaacgg tcccacgccg
     1801 cagccggccg tgaggtaggc gccggcgccg gtggccacga cgccgacgcc aacggccagc
     1861 agcagccagg cctggagcgg gttccccggc gcgggcagca gccccatggt ggcttgcacc
     1921 accgggccga cgcacagcgg ggcgaacgtg ccccagcgcg gccggccacc cagcgcgaca
     1981 cccgcggcca gcgtgaccgc cccggtgccc gcgatggcgg cccacagcgg cacgccgaag
     2041 gccccggaca cgccgacgtg gaaggcgtcc caggcgccca ccccgaggcc ggaggacagc
     2101 atcgccccga cgcccgtgcc gatcagcagg acgcccgcgg cgagctggcc caggcgcacc
     2161 gagccgacgg cggccggccc gggcgaggcc cacacggcgg ccgggccccg gccggcgcgc
     2221 ggcacggcgg cgacgcacgc gcgcaggaat gcacacagca atgacgacat gacggaaaga
     2281 actcctgtgg tggcccgcat ggcccgcgaa tggcgccgag gaagcccgcg tcccgctcgg
     2341 ggaccttgga atcaatcggc gcggtgaggt ctgtcaggcg aggctatcga cggcctgcag
     2401 gcccgctgcg gccggaggcg tccggacgga catctcaccg gcggttacac ccgtccgggc
     2461 tcaggcgtgg cggcggaccc actcgtgcat ggccacgccg gaggccacac cggcgttgat
     2521 ggagcgcgtg gagccgaact gcgggatgct caccaccacg gcggcggcct cgcgcgccgc
     2581 ctgggacagc ccggggccct cctggccgaa cagcagcacg cacgcgcgcg gcagatcggc
     2641 ctgggcgagc ggcacggcgc ccggcaggtt gtccacgccc accagcggca ggccggcgtc
     2701 ctgcgcccac gccaccaggt caccgacggt gtcgtgatgg acgacgtgct ggtaggtctc
     2761 ggtcatcatc gcgccgcgcc gattccactt gcgcctgccc acgatgcgca cgctggcggc
     2821 gttgaacgcg ttggcgttgc gcaccacggt gccgatgttg aagtcgtgcg tccagttctc
     2881 gatcgccacg tggaaggggt gccgccgcgt gtccaggtcg gcggtgatcg cctccaccgt
     2941 ccagtagcgg tagcggtcgg tcacgttgcg gcggtcacca tgggcgagca ggtccgggtc
     3001 cagccggggg tcgtccggcc acgggccctc ccacgggccc acgccgacca ccggaccggt
     3061 gacgcctgag gcctgctcgt cgctcatgcg gcgaggctag tgcctgctac ccggttggcc
     3121 gaccccatgc ggtggggatg cacccggaac ggcagtagca tcagccccgg tcgcagccac
     3181 gatgcggcag agggccgcct cggaccatga gcgaacgaga aacgcggtga cgcgcctgat
     3241 gggcgacaac gagcagaacc cggagttcgg agccaacagc tggctcgtcg aggagatgta
     3301 ccgccagtac ctcgc
//
LOCUS       DATMKC010000425         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_70542, whole genome shotgun sequence.
ACCESSION   DATMKC010000425 DATMKC010000000
VERSION     DATMKC010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70542"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /gene="dop"
                     /locus_tag="VNU01_12425"
     CDS             complement(<1..1466)
                     /gene="dop"
                     /locus_tag="VNU01_12425"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: protein motif:HMM:TIGR03688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HWH33467.1"
                     /translation="MALPKYLGTETEFGITVVGRPDFNPVLASSMVVNAYAADGRHRA
                     RWDYEEENPLRDARGFTQGHMAEQPPEDDLGLANSILPNGARFYVDHAHPEYSSPECS
                     NPRDAVIWDKAGERILEIAAAKAADLLPSRDAGREGRILITKNNTDGKGAAYGMHENY
                     LVDRRVPFGEIVRQITPFFVTRQVLVGAGRIGNEFDLDDVPFQISQRADFFEVEVGLE
                     TTLKRPIVNTRDEPHADPDRFRRLHVIIGDANMSEVCTYLKLGSTALLLSMIEDGALP
                     DPPSLAAPVKALHAVSHDLTCRRALQLKDGRALSPLEIQWHYLEQAKKYCRTADAPVW
                     GEQVTETWERILSALEDDPMSLDGVVDWVTKYRILQRYVERDGLDWSDDKLKLIDVQY
                     HDVRQDKGLYNRLVATGRMERMVDEAEVQAAIGLPPADTRAWFRGRCLDKFRDQVAAA
                     GWDSIIFDTGRDTLQRVPMMDPGRGGRAMTQDLIDRCET"
     gene            complement(1502..>2548)
                     /gene="arc"
                     /locus_tag="VNU01_12430"
     CDS             complement(1502..>2548)
                     /gene="arc"
                     /locus_tag="VNU01_12430"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HWH33468.1"
                     /translation="LHDQIEAIQDAVELPFLHADLFVEHELRPPKGILLYGPPGCGKT
                     MIAKAVANSLAKRVAEKEGRADARSYFLNIKGPELLNKYVGETERQIRLIFQRAREKV
                     EEGFPVIVFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVETLKDVIVIGASNRE
                     DMIDPAILRPGRLDVKIKVERPDAEAARDIFAKYIVEDLPLHPDLVKEYGDEEKAVDA
                     LVEETVERMYAEADENRFLEVTYANGDKELLYFKDFNSGAMIENVVARAKKFAIKRYL
                     DEGQKGIKAEDLYQAIRDEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLIGDGKE
                     PGKSIENVNTGQYL"
BASE COUNT          336 a          890 c          853 g          469 t
ORIGIN      
        1 gtctcgcagc ggtcgatcag gtcctgggtc atcgcccgtc caccccggcc cgggtccatc
       61 atcggtacgc gctgcagcgt gtcgcgaccg gtgtcaaaga tgatcgagtc ccagccggcc
      121 gccgcgacct ggtcgcggaa cttgtccagg caccggccgc ggaaccacgc gcgcgtgtcc
      181 gccggaggca ggccgatcgc cgcctgcacc tccgcctcgt cgaccatccg ctccatgcgg
      241 ccggtggcga ccagccggtt gtacaggccc ttgtcctggc gcacgtcgtg gtactgcacg
      301 tcgatgagct tgagcttgtc gtcggaccag tccaggccgt cgcgctccac gtagcgctgg
      361 aggatgcggt acttggtcac ccagtccacc acgccgtcca gcgacatggg gtcgtcctcg
      421 agcgcggaca ggatgcgctc ccacgtctcg gtgacctgct ccccccacac cggcgcgtcc
      481 gcggtgcggc agtacttctt ggcctgctcc aggtagtgcc actggatctc cagcgggctg
      541 agcgcgcggc cgtccttgag ctgcagcgcg cgccggcagg tcaggtcgtg cgacaccgcg
      601 tgcagcgcct tcaccggcgc cgccagcgac ggcgggtcgg gcagcgcgcc gtcctcgatc
      661 atggacagca gcagggccgt ggagcccagc ttgaggtacg tgcacacctc gctcatgttc
      721 gcgtcgccga tgatgacgtg gaggcggcgg aagcggtccg ggtcggcgtg cggctcgtcg
      781 cgcgtgttca cgatcggacg cttcagcgtg gtctccagcc cgacctcgac ctcgaagaag
      841 tcggcgcgct ggctgatctg gaacggcacg tcgtccaggt cgaactcgtt gccgatgcgc
      901 ccggcgccca ccagcacctg ccgcgtgacg aagaacgggg tgatctggcg cacgatctcc
      961 ccgaagggca cccggcggtc caccaggtag ttctcgtgca tgccgtaggc ggcgcccttg
     1021 ccgtcggtgt tgttcttggt gatcaggatg cggccctcgc ggccggcgtc gcggctcggc
     1081 agcaggtcgg cggccttggc cgcggcgatc tccaggatcc gctcccccgc cttgtcccag
     1141 atcaccgcgt cgcgcgggtt ggagcactcc ggcgagctgt actcggggtg ggcgtggtcg
     1201 acgtagaagc gcgccccgtt cggcaggatc gagttcgcca ggcccaggtc gtcctccggc
     1261 ggctgctcgg ccatgtggcc ttgcgtgaac ccgcgcgcgt cccgcagcgg gttctcctcc
     1321 tcgtagtccc agcgggcccg gtgccggccg tcggcggcgt aggcgttgac caccatggac
     1381 gaggccagca cggggttgaa gtccgggcgg ccgacgaccg tgatcccgaa ctccgtctcg
     1441 gtgcccaggt acttcggcag cgccaagaag actccctacg gtccgacccg ggcggtgcgc
     1501 cctacaggta ctggccggtg ttgacgttct cgatggactt gccgggctcc ttgccgtccc
     1561 cgatgagcgt gcgcacgtac acgatgcgct cgcccttctt gcccgagatg cgggcccagt
     1621 cgtcggggtt cgtggtgttc ggcaggtcct cgttctcctt gaactcgtcg cggatcgcct
     1681 ggtagaggtc ctcggccttg atgcccttct ggccctcgtc caggtagcgc ttgatcgcga
     1741 acttcttcgc gcgggcgacc acgttctcga tcatggcgcc ggagttgaag tccttgaagt
     1801 acagcagctc cttgtcgccg ttggcgtagg tgacctccag gaagcggttc tcgtcggcct
     1861 cggcgtacat gcgctccacg gtctcctcga ccagcgcgtc gaccgccttc tcctcgtcgc
     1921 cgtactcctt gaccaggtcg gggtgcagcg gcaggtcctc gacgatgtac ttcgcgaaga
     1981 tgtcgcgggc cgcctccgcg tccgggcgct cgaccttgat cttcacgtcc aggcgcccgg
     2041 gccgcaggat cgccgggtcg atcatgtcct cgcggttgga cgcgccgatg acgatcacgt
     2101 ccttcaaggt ctccacgccg tcgatctcgc tcagcagctg cgggacgatc gtgttctcca
     2161 cgtcgctgga cacgccggag ccgcgcgtgc ggaagatcga gtccatctcg tcgaagaaca
     2221 cgatcacggg gaagccctct tcgaccttct cccgcgcgcg ctggaagatc aggcggatct
     2281 ggcgctcggt ctccccgacg tacttgttca aaagctccgg gcccttgatg ttgaggaagt
     2341 agctgcgcgc gtcggcgcgg ccttccttct ccgccacgcg cttggccagg ctgttggcca
     2401 ccgccttggc gatcatcgtc ttgccgcagc ccggcgggcc gtacagcagg atgcccttgg
     2461 gcgggcgcag ctcgtgctcg acgaacaggt cggcgtgcag gaacggcagc tccacggcgt
     2521 cctggatcgc ctcgatctgg tcgtgcag
//
LOCUS       DATMKC010000426         2744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_58814, whole genome shotgun sequence.
ACCESSION   DATMKC010000426 DATMKC010000000
VERSION     DATMKC010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58814"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VNU01_12435"
     CDS             complement(<1..838)
                     /locus_tag="VNU01_12435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWH33469.1"
                     /translation="MAGAGELRVLRRRIRSVKSTQKITRAMELIAASRITRARNAVEA
                     ARPYAEMVTQVIRDLATEASLRDHPLLAAREQVNAVGVLVITSDRGLAGAYNTNVIRR
                     AERLVKEEQEAGRQVRLYAIGRKAEVYFRYRGMAPAQVWTGVSDQPGYDAAQQVGRAV
                     TEDYVSGRIDRVWMAYTDFRSALTQVASTARLLPVDRAQLEGGTAFPPQFMFEPEPGA
                     ILDLLIPKYVEQRVFAGMLESAASEHASRQRAMKAATDNAGEIIDALTRDANSARQAA
                     ITT"
     gene            complement(852..2582)
                     /gene="atpA"
                     /locus_tag="VNU01_12440"
     CDS             complement(852..2582)
                     /gene="atpA"
                     /locus_tag="VNU01_12440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWH33470.1"
                     /translation="MTELSISPEDIAGVLHSKIEGYTASLEREQVGRVIETGDGIARI
                     TGLPGVMANELLDLGVGSDGQRLYGLALNLEEDTIGAVIMGDNADVEEGQIVKPTGQV
                     LSVPVGDAYLGRVVDALGRPIDGKGPLDESKLAGRRGLEVQAPSVVDRQPVHEPLQTG
                     IKAVDAMTPIGRGQRELIIGDRQVGKTAVAIDAIINQRENWATGDPKKQVKCIYVAVG
                     QKGSTVAEIVNRLEQSGAMEYTTVVSAPASAPAPFQYIAPYTGAAIGSYWMYEGQHGL
                     VVYDDLSKQADAYRQISLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDELGAGSLTA
                     LPIIETKGGDVSAFIPTNVISITDGQIYLETDLFFQGVRPAINVGISVSRVGGSAQIK
                     AMKKVAGTLRLDLAQFRELEAFAQFGSELDKASQAQIDRGQRLVELLKQPQYAPVPVE
                     EQVVVIWAGTNGKVDDIPVPDVRRFETELREYLRSRHADLLAGIRSDGKLEDERMAEL
                     DKAVEAFKSTFQPSQQVAVAEPHAMEDLPSLSPDGGADSDADQPAETDRTDTEERMGP
                     VSDTDAPPVP"
BASE COUNT          343 a          964 c          984 g          453 t
ORIGIN      
        1 cggtggtgat cgccgcctgc cgcgcgctgt tcgcgtcgcg ggtcagggcg tcgatgatct
       61 cgccggcgtt gtccgtcgcg gccttcatgg cgcgctggcg gctggcgtgc tcgctcgccg
      121 cgctctccag catcccggcg aacacgcgct gctcgacgta cttggggatg agcaggtcca
      181 ggatcgctcc cggctccggc tcgaacatga actgcggcgg gaaggcggtg ccgccctcca
      241 gctgcgcgcg gtcgaccggc agcaaccgcg ccgtggaggc gacctgcgtc agcgccgagc
      301 ggaagtcggt gtaggccatc cacacgcggt cgatgcgccc ggagacgtag tcctcggtga
      361 cagcccggcc cacctgctgc gccgcgtcgt agcccggctg gtcgctgaca ccggtccaca
      421 cctgggcggg cgccatgccg cggtagcgga agtacacctc ggccttgcgg ccgatcgcgt
      481 acaggcgcac ctggcggccg gcctcctgct cctccttcac gaggcgctcc gcacggcgga
      541 tgacgttggt gttgtaggcg ccggccagtc cgcggtcgga ggtgatcacc agcacgccga
      601 cggcgttgac ctgctcacgc gcggccagca gcgggtggtc gcgcaggctc gcctccgtgg
      661 ccaggtccct gatgacctgc gtcaccatct ccgcgtacgg gcgcgcggcc tcgaccgcgt
      721 tgcgcgcgcg cgtgatgcgg ctcgccgcga tgagctccat cgcccgcgtg atcttctgcg
      781 tcgacttgac gctgcggatc cgccggcgaa ggacgcgaag ctctcccgct cctgccatgt
      841 cgtgtgctct cctacggcac cggcggagcg tcggtgtccg agaccggacc catgcgctcc
      901 tcggtgtcgg tccggtccgt ctcggccggc tggtcggcgt cgctgtcggc ccctccatct
      961 gggctcaggc tgggcaggtc ctccatcgcg tgcggctcgg ccacggccac ctgctgggac
     1021 ggctggaacg tgctcttgaa cgcctccacc gccttgtcca gctcggccat gcgctcgtcc
     1081 tcgagcttgc cgtcggagcg gatgcccgcg agcaggtccg cgtgacggct gcgcaggtac
     1141 tcgcgcagct cggtctcgaa gcggcgcacg tccgggaccg ggatgtcgtc caccttgccg
     1201 ttggtgccgg cccagatgac gaccacctgc tcctccacgg gcaccggtgc gtactgcggc
     1261 tgcttgagca gctccaccag gcgctggccg cggtcgatct gcgcctgcga ggccttgtcc
     1321 agctcagagc cgaactgggc gaacgcctcc agctcgcgga actgggccag gtccaggcgc
     1381 agcgtgccgg ccaccttctt catggccttg atctgcgcgg agccgcccac gcgggacacc
     1441 gagatgccca cgttgatggc ggggcgcacg ccctggaaga acaggtcggt ctccaggtag
     1501 atctgcccgt cggtgatcga gatcacgttg gtcgggatga acgcggagac gtcgccgccc
     1561 ttggtctcga tgatcggcag cgcggtgagc gacccggcac ccagctcgtc ggacagcttc
     1621 gcggcgcgct ccagcaggcg ggagtgcagg tagaacacgt cgcccgggta ggcctcgcgg
     1681 cccggcgggc ggcgcagcag cagcgagatc tgccggtagg cgtcggcctg cttggacagg
     1741 tcgtcgtaca cgaccagccc gtgctggccc tcgtacatcc agtaggagcc gatcgccgcg
     1801 ccggtgtagg gggcgatgta ctggaacggt gccggcgcgg acgcgggcgc ggagacgacc
     1861 gtcgtgtact ccatcgcgcc ggactgctcc aggcggttca cgatctcggc gaccgtggag
     1921 cccttctggc ccacggcgac gtagatgcac ttgacctgct tcttcgggtc gccggtcgcc
     1981 cagttctcgc gctggttgat gatcgcgtcg atggcgacgg cggtcttgcc gacctggcgg
     2041 tcaccgatga tcagctcgcg ctgcccgcgc ccgatcggcg tcatcgcgtc gacggccttg
     2101 atgccggtct ggagcggctc gtgcaccggc tggcggtcga ccaccgacgg ggcctgcacc
     2161 tccaggccgc ggcggccggc gagcttggac tcgtccagcg ggcccttgcc gtcgatgggg
     2221 cggcccaggg cgtccaccac gcggcccagg taggcgtcgc ccacggggac cgacagcacc
     2281 tggccggtgg gcttgacgat ctggccctct tcgacgtcgg cgttgtcgcc catgatcacg
     2341 gcgccgatgg tgtcctcttc gaggttcagc gccaggccgt acaggcgctg gccgtccgag
     2401 ccgacgccca ggtcgagcag ctcgttggcc atgacgccgg gcaggccggt gatgcgcgcg
     2461 atgccgtcac cggtctcgat cacgcggccg acctgctcgc gctcgaggct cgcggtgtag
     2521 ccctcgatct tcgagtgcag gacgccggcg atgtcctccg gggagatgct cagctcagtc
     2581 atcggtgtcg ctccagctgg agtggatggt ccggggtgtc aggggggcgt cagggggcgt
     2641 caggccccgg tgaggcgggc cttcagttcg gtgaggcgtc gggacacgct gccgtcgatc
     2701 acggtgtcgc cgatgcggac cgacaggccg ccgacgacct ccgg
//
LOCUS       DATMKC010000427         2797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_58845, whole genome shotgun sequence.
ACCESSION   DATMKC010000427 DATMKC010000000
VERSION     DATMKC010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58845"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2333
                     /gene="treY"
                     /locus_tag="VNU01_12445"
     CDS             <1..2333
                     /gene="treY"
                     /locus_tag="VNU01_12445"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HWH33471.1"
                     /translation="PRPCALAASAHGRGLGVVVDIVPNHVGVHHTNAMWDGLLARGPA
                     GDEARCFDVNWDSALPGSAQKVILPVLGEQYGEVLHSGELEVLRDSGSDPGRGWRLRY
                     HEHDFPLSEESCQALERSGGGEALRGEPGVPDTWQRLHALLERQHYRLVHWRVGHRVI
                     NYRRFFAVDTLAAVRVEDEVVFDRVHGKILQLVGDGVVDGLRVDHPDGLRDPGRYLER
                     LRERAGGVWIVVEKILQHAEALRDWPVEGTTGYEFCNDVLGLFVDPEGEAPLSELDRE
                     LGGRPDELGRVERECKRQILAEDLASEHQRLTGMLWRLAQEHPTVRDLDDRHVAEAIA
                     GAIAHMEVYRAYIDPQTGRATPEDVERVEGAVERARAAARGGGQARRGQAGPPDEVFD
                     LVAAAASGRLGTTVAHLDFAARFAQLSSAVMAKGVEDTAFYRYQRLLALNEVGGHPGR
                     FGTDAAAFHRANAQRQACHPAGMLTTATHDTKRGEDARLRVAALSERPAEWAALARRW
                     APGAPDGHAASLALQTLVAVWPLAGGPPDEEVRRRVHAYLVKALREARLRTDWAEPDE
                     GYELGVAEWLDGLLGDDGFVGEFGGFVGGVQEIAMTAGVAQTLLRLTSPGVPDTYQGC
                     ELWEDALVDPDNRRAVDFGARERLLAELGARPDAAALWAARRDGRVKLWVLRRALQVR
                     NTRPACFGPDAGYEPLRAEGAMADHVVAFARTSPDGERVITVAPRLPGRVLAQGWGDT
                     TLDLGGTGYVDALTGRPATDRLADLTATLPVALLTR"
     gene            complement(2392..>2797)
                     /locus_tag="VNU01_12450"
     CDS             complement(2392..>2797)
                     /locus_tag="VNU01_12450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWH33472.1"
                     /translation="AQRRGVPRWHLEAGGAVLDQLAEAAGTPLVVADTGGLGELVEHG
                     ATGLKVPPRDTAALREAIATVLTDRRRARRMAVAAHDQLERRFSWESIAAQTLRTYER
                     AVREERELAHAHRPPLRLIFDRSPLLGGSAEA"
BASE COUNT          328 a         1002 c         1139 g          328 t
ORIGIN      
        1 ggccccgccc gtgcgccctg gcggccagcg cgcacgggcg gggcctgggc gtggtcgtcg
       61 acatcgtgcc caaccacgtc ggcgtgcatc acaccaacgc catgtgggac gggctgctcg
      121 cccgcggccc ggcgggcgac gaggcgcgct gcttcgacgt caactgggac tccgcgctgc
      181 ccggctccgc gcagaaggtg atcctgccgg tgctgggcga gcagtacggc gaggtgctgc
      241 acagcggcga gctggaggtc ctgcgggact ccggctcgga ccctgggcgc ggctggcgcc
      301 tgcgctacca cgagcacgac ttcccgctgt cggaggagtc ctgccaggcg ctggagcgct
      361 ccggcggcgg cgaggcgctg cgcggcgagc cgggcgtgcc ggacacgtgg cagcgcctgc
      421 acgcgctgct ggagcgccag cactaccgcc tggtgcactg gcgcgtcggc catcgcgtca
      481 tcaactaccg gcgcttcttc gccgtcgaca cgctggccgc ggtgcgcgtg gaggacgagg
      541 tggtgttcga ccgcgtccac ggcaagatcc tccagctcgt cggcgacggc gtggtggacg
      601 gcctgcgcgt cgaccacccg gacggcctgc gcgatcccgg gcgctacctg gagcgcctgc
      661 gcgagcgcgc cggcggcgtg tggatcgtgg tggagaagat cctgcagcac gccgaggcgc
      721 tgcgggactg gcccgtggag ggcacgaccg gctacgagtt ctgcaacgac gtgctgggcc
      781 tgttcgtgga cccggagggg gaggcgccgc tgagcgagct cgaccgggag ctgggcgggc
      841 gcccggacga gctgggccgc gtcgagcggg agtgcaagcg gcagatcctg gccgaggacc
      901 tcgcctcgga gcatcagcgg ctcacgggga tgctgtggcg gctggcgcag gagcacccca
      961 ccgtgcgcga cctcgacgac cgccacgtcg cggaggcgat cgctggggcc atcgcccata
     1021 tggaggtcta ccgcgcctac atcgatcccc agacgggccg cgcgacgccg gaggacgtcg
     1081 agcgcgtgga gggcgccgtc gagcgcgccc gtgcggcggc gcgcggcggc gggcaggcgc
     1141 gccgcgggca ggcgggccct cccgacgagg tgttcgacct ggtcgcggcg gcggcctcgg
     1201 ggcggctggg caccacggtg gcgcacctgg acttcgccgc gcgcttcgcg cagctgtcca
     1261 gcgccgtcat ggccaagggc gtggaggaca ccgccttcta ccgctaccag cgcctgctgg
     1321 cgctgaacga ggtgggcggg cacccggggc ggttcggcac cgacgcggcg gcattccacc
     1381 gcgcgaacgc ccagcggcag gcgtgccacc ccgccgggat gctgaccacc gccacgcacg
     1441 acacgaagcg cggcgaggac gcgcgcctgc gcgtggcggc gctgagcgag cgtccggcgg
     1501 agtgggcggc gctggcgcgg cgctgggcgc ccggcgcgcc ggacgggcat gccgcgtccc
     1561 tggcgctgca gacgctggtg gcggtgtggc cgctggccgg cgggccgccg gacgaggagg
     1621 tgcgccgccg cgtgcacgcc tacctggtga aggcgctgcg ggaagcgcgg ctgcgcacgg
     1681 actgggccga gcccgacgag ggctacgagc tcggcgtggc cgagtggctg gacggcctgc
     1741 tgggcgacga cggtttcgtg ggtgagttcg gcggcttcgt ggggggcgtg caggagatcg
     1801 cgatgacggc cggcgtggcg cagacgctgc tgcgcctgac ctcgcccggc gtgccggaca
     1861 cctaccaggg ctgcgagctg tgggaggacg ccctggtgga ccccgacaac cgccgcgccg
     1921 tggacttcgg cgcgcgggag cggctgctgg cggagctggg cgcgcggccg gacgcggcgg
     1981 cgctgtgggc ggcgcggcgc gacggccgcg tgaagctgtg ggtgctgcgc cgggcgctgc
     2041 aggtgcgcaa cacccgcccg gcctgcttcg gcccggacgc gggctacgag ccgctgcgcg
     2101 ccgagggcgc catggcggat cacgtcgtgg ccttcgcccg cacctcgccg gacggggagc
     2161 gggtgatcac cgtggccccc cgcctgcccg ggcgcgtcct cgcccagggc tggggcgaca
     2221 ccacgctcga cctcggcggg accggctacg tcgacgcact caccggacgg cccgcgaccg
     2281 accggctggc cgacctcacc gcgacgctcc ccgtcgcgct gctcacccgg tagcggggag
     2341 tcctccggcg ggataccgcc gccccactcc ccgttgcgaa ggggagacgg gtcaggcctc
     2401 ggcggagccg cccagcaggg gggagcggtc gaagatgagc cgcaggggcg ggcggtgggc
     2461 gtgggcgagc tcgcgctcct cgcgcaccgc gcgctcgtag gtccgcagcg tctgcgccgc
     2521 gatggactcc caggagaagc ggcgctcgag ctggtcgtgg gcggcgaccg ccatgcgccg
     2581 cgcgcggcgg cggtcggtga gcaccgtcgc gatcgcctcg cgcagcgccg cggtgtcccg
     2641 cggtggcacc ttgaggccgg tggcgccgtg ctcgaccagc tcgccgaggc cgccggtgtc
     2701 cgccacgacc agcggcgtcc cggcggcctc ggcgagctgg tcgagcacgg cgccaccggc
     2761 ctcaaggtgc caccgcggga caccgcggcg ctgcgcg
//
LOCUS       DATMKC010000428         3091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_23374, whole genome shotgun sequence.
ACCESSION   DATMKC010000428 DATMKC010000000
VERSION     DATMKC010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23374"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VNU01_12455"
     CDS             complement(<1..1778)
                     /locus_tag="VNU01_12455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWH33473.1"
                     /translation="MPADPAAAPPARLPERHWPTVSVVLINYNGKDYLERCLGSLRAQ
                     EYPQDRIEIVFIDNNSSDGSVEYVREAFPEARVVVNDTNTGFSPAVNQGAQLAGGKYL
                     ALLNNDAEADSRWLIEAVQVLEMEDRVACVASKILRDDRRTIDYAGGQMAFYGHGFAK
                     GNKDLDSPAGESEDEGNRPTLFASGGAMIVRRDVFLATGAFDESYFAFFEDVDFGWRL
                     WVLGHDVLYVPRSKVYHRHHGTIERFGYPRERYLLERNALASVFKNYSDDMLARVLPG
                     TLVLALLRGLADEETELPDYRIREDVDPIRSAEVSPLTGAHLAAIRDWGLALGQLGAK
                     RYAIQSRRKRDDREIMRLFEEALLPNVPDPDFMQVFAYVLDAWNLHEHAKPKGHILIL
                     TADRLGKKMAGPAIRCWEMAKLLSKTHRVTLGTTTPPELDHPDFDVVQVNSERIDALL
                     PDTDVVIFQGFIMFFYPQIAASDVPVLVDIYDPFHLEGLELRKEEAPIERFATARSDT
                     KILNDQLERGDFFVCASEKQRDFWLGQMTAIGRINPATYDQDASLRKLLDVAPFGLPT
                     EAPQKVDKVVKGVIPGIEEDDFLLLWG"
     gene            1951..2976
                     /locus_tag="VNU01_12460"
     CDS             1951..2976
                     /locus_tag="VNU01_12460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWH33474.1"
                     /translation="MDQPSSGALLSAVVPVFNEVDCLPAFHAELVEALDALGRPAEIL
                     YVDDGSSDGSRDLLERLCAQRPDLVRVLVLRRNFGKSGALAAGFDHARGELIVMMDAD
                     GQDVPAELPKLLRLMEQDGYDLVGGWRRERIDRFVKRVTSRWYNAATRSMTGLEINDF
                     NTGFKLLRAEVAAELPLYGEFHRFVPVLAHDLGFKVGEVAVRHRPRQAGKSKYLSLTR
                     FPKTMLDLLTVLFLTRFADRPLYLFGGVGAGLLGLGSLALVYLAGLKLFTGAGIGARP
                     LLQFGVLAVLIGVQLIGTGFIGDVLRHSFARDRKPYRVRHVLGAGASAGGGPGQDETE
                     PPARAAG"
BASE COUNT          416 a         1046 c         1125 g          504 t
ORIGIN      
        1 ccccacaaca gcaggaagtc gtcctcctcg atgccgggga tgacgccctt gaccaccttg
       61 tcgaccttct gcggcgcctc ggtcggcagg ccgaacggcg cgacgtccag cagcttgcgc
      121 aggctggcgt cctggtcgta ggtggccggg ttgatgcggc cgatcgcggt catctggccc
      181 agccagaagt cgcgctgctt ctcgctggcg cacacgaaga agtcgccgcg ctccaactga
      241 tcgttgagga tcttggtgtc gctgcgcgcg gtggcgaagc gctcgatcgg cgcctcctcc
      301 ttgcgcagct ccaggccctc caggtggaac gggtcgtaga tgtcgaccag gacgggcacg
      361 tccgaggcgg cgatctgcgg gtagaagaac atgatgaagc cctggaagat caccacgtcg
      421 gtgtcgggca gcagggcgtc gatgcgctcg gagttgacct gcaccacgtc gaagtccggg
      481 tggtccagct ccggcggggt ggtggtgccc agggtcacgc ggtgggtctt gctgaggagc
      541 ttcgccatct cccagcagcg gatcgccggg ccggccatct tcttgcccag gcggtcggcg
      601 gtgaggatga ggatgtggcc cttgggcttg gcgtgctcgt gcaggttcca ggcgtccagc
      661 acgtaggcga acacctgcat gaagtccggg tccggcacgt tgggcagcag cgcctcctcg
      721 aacaggcgca tgatctcgcg gtcgtcgcgc ttgcgccggc tctggatggc gtagcgcttc
      781 gcgccgagct ggcccagcgc caggccccag tcgcggatcg ccgccaggtg ggcgcccgtg
      841 agcggcgaca cctcggcgct gcggatcggg tcgacgtcct cgcggatgcg gtagtcgggg
      901 agctcggtct cctcgtcggc caggccacgc agcagcgcca gcaccagcgt gccgggcagc
      961 acccgggcga gcatgtcgtc ggagtagttc ttgaacacgc tggccagcgc gttgcgctcc
     1021 agcaggtagc gctcgcgcgg gtagccgaag cgctcgatgg tgccgtgatg gcggtggtag
     1081 accttggagc gcggcacgta gagcacgtcg tggcccagca cccacaggcg ccagccgaag
     1141 tcgacgtcct cgaagaacgc gaagtagctc tcgtcgaacg cgccggtggc caggaagacg
     1201 tcgcggcgca cgatcatcgc gccgccggag gcgaacagcg tcgggcggtt gccctcgtcc
     1261 tcagactctc cagctgggga gtccaggtcc ttgttgccct tggcgaagcc gtggccgtag
     1321 aaggccatct gcccaccggc gtagtcgatg gtgcggcggt cgtcgcgcag gatcttgctg
     1381 gcgacgcagg cgacgcggtc ctccatctcc aggacctgca cggcctcgat cagccagcgc
     1441 gagtcggcct ccgcgtcgtt gttgagcagc gccaggtact tgccgccggc cagctgcgcg
     1501 ccctggttca ccgccgggct gaagccggtg ttggtgtcgt tgacgacgac ccgcgcctcg
     1561 gggaacgcct cgcgcacgta ctccacggag ccgtccgagg agttgttgtc gatgaagacg
     1621 atctcgatgc ggtcctgcgg gtactcctgc gcgcgcagcg agcccaggca gcgctccagg
     1681 tagtccttgc cgttgtagtt gatgaggacc accgacacgg tgggccagtg ccgctccgga
     1741 aggcgggcgg gcggcgcggc ggcgggatct gcgggcatgg gtgtcgctcc tggcggaggc
     1801 cggggtcggt gatcagtagg tgtctaccac agccgcaggt tccatccggg tgaccttcag
     1861 tccatgggtg gttgggtgcg ctttcagagc gatggctctg aaaccgcacc cgcgtggggc
     1921 gaaaagctgt ggaccgctac gatcccgtgc atggatcagc ccagttccgg cgctctgctc
     1981 agcgccgtcg tccccgtctt caacgaggtc gactgcctgc ccgcgttcca cgccgagctg
     2041 gtggaagcgc ttgacgccct gggccgcccg gcggagatcc tctacgtcga cgacggctcc
     2101 agcgacggct cgcgcgacct gctggagcgc ctctgcgcgc agcggcccga cctggtgcgc
     2161 gtcctggtgc tgcggcgcaa cttcggcaag tccggcgcgc tggccgccgg gttcgaccac
     2221 gcccgcggcg agctgatcgt gatgatggac gccgacggcc aggacgtgcc ggcggagctg
     2281 cccaagctgc tgcggctgat ggagcaggac ggctacgacc tggtcggcgg gtggcggcgc
     2341 gagcgcatcg accgcttcgt caagcgcgtc acgtcgcgct ggtacaacgc cgcgacgcgc
     2401 tccatgaccg ggctcgagat caacgacttc aacacgggct tcaagctgct gcgcgcggag
     2461 gtggccgcgg agctcccgct gtacggggag ttccaccgct tcgtgccggt gctggcccac
     2521 gacctcggct tcaaggtcgg cgaggtggcg gtgcgccacc ggccgcgcca ggcgggcaag
     2581 agcaagtacc tgtcgctgac ccgcttcccc aagacgatgc tggacctgct gaccgtgctg
     2641 ttcctgacgc gcttcgccga ccgcccgctg tacctgttcg gcggcgtcgg ggcggggctg
     2701 ctcggcctcg gctcgctggc gctggtgtac ctggcggggc tcaagctctt cacgggcgcc
     2761 ggcatcggcg cgcggccgct gctgcagttc ggcgtgctgg ccgtgctgat cggcgtgcag
     2821 ctcatcggca cggggttcat cggcgacgtg ctgcgccact cgttcgcgcg cgaccgcaag
     2881 ccctaccgcg tgcgccacgt cctcggcgcg ggcgccagcg cgggcggcgg ccccggccag
     2941 gacgagaccg agccgcccgc gcgcgcggct ggctagcgca ccgtgcgcgt cctggccctc
     3001 acgcacgtct tcccgcgggc ggcggacgac ccgtcggcgc cgttcctgct cacgtgggcg
     3061 cgggcgctgc gcgacgccgg cgtcgacgtc g
//
LOCUS       DATMKC010000429         4344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_306202, whole genome shotgun sequence.
ACCESSION   DATMKC010000429 DATMKC010000000
VERSION     DATMKC010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306202"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VNU01_12465"
     CDS             complement(<1..889)
                     /locus_tag="VNU01_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWH33475.1"
                     /translation="MSRATRLVLGGAGLGAAAVAWAAARSRPELPDAAPPVPPPGLPE
                     GRLVAVPGRGELFVRDTGPTEHAPGGAPTILLLHGWMFPSDLNWFPSYEPLSALGRVI
                     ALDHRGHGHGIRPSKPFRLADAADDAAALVRHLGTGPVVAVGYSMGGPIAQLLAVRHA
                     ELVRGVVLCATSDTFSQTARDRALWRTMGALQLILRLLPRHTVERLVRAQALGRLPMR
                     ITTLLKDDTPPEIVALIPWFLGELARGTAEDIAEAGRELGRYDGTGFVGRITAPTVAI
                     VTTEDELVPVDRQRRMASRI"
     gene            1163..1357
                     /locus_tag="VNU01_12470"
     CDS             1163..1357
                     /locus_tag="VNU01_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33476.1"
                     /translation="MKALVGAWDSRPQHVLAELAALRGRIKQLEQELARTREENAALR
                     SEREDGMDIEIALTPASASR"
     gene            1594..>4344
                     /locus_tag="VNU01_12475"
     CDS             1594..>4344
                     /locus_tag="VNU01_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWH33477.1"
                     /translation="MFLKSLTLRGFKSFADKTTLEFEPGITVIVGPNGSGKSNIVDAF
                     AWVFGTHSAKSLRGGTMADVIFAGAPGKPALGRASVELTIDNSAGQLPIEFSEVTISR
                     SMFSSGENEYRINNVACRLLDVQELLSDTGLGRENHTIVGQGQLDAVLNAKPEERRAF
                     IEEAAGILKHRRRKERALRKLSQMEQHLERLNDVLRELRRNLRPLERQAEAAAKHAQL
                     QEQLKAVRVDRALRELAVLDARWTTESGEQTRSDERLAVLEGDLATGRSRESELDQAL
                     GALAPAVREAGQTHFRLANLVERYRGLSGRIEERRAGLLEAVEEPVAGRDPEELRARA
                     RGERGALEELEAERSGAHSALEQAEEARREAERARRAHEQAAAAEARRRAEVREHRLR
                     WEGEVSALRSSLAQASGEEGRLASQVSGVESRKAELERDVETVTREIQRLDSDAAVLA
                     EQLGASEQVLERRHEQQKAAALRERELERQRASLEARADALRAASQEATEGSAALLAG
                     AERGEVSGVVGALADQVSVAEPSLAKAVAAALGPLGDALVVRDRRSARDAVGFVRSRQ
                     SGRVLLLVGDGAQPPAEDAEAVAELAMLGARPVADVLRAEPSLHAALRQALAGVYVVD
                     ATDDGWGAVCKVAERYPALVFVSADGDVAGPRGYAGGSATANSAVLSKAAAEQAEAKL
                     AEVSDELLRAHRQLADADRELEAAKREYDAAVAAMQESDGQVTAAAERLKRLRKELET
                     CERELGVLHGQQEDLSREMGRQRERLADLESRGVDALPEPEPDGPDLEAERLDDLLAE
                     AREREVQARLEVSAVEQRAGEVARRIDAYEREAAEVEHQLVERERRRVARLAAIERCG
                     ELLVLAGLALARSEQSLALAESERDRLEEARAAQQRELGVVRARIRELEEELGVLREA
                     RHA"
BASE COUNT          584 a         1566 c         1704 g          490 t
ORIGIN      
        1 ggatgcggga ggccatgcgg cgctggcggt cgacggggac cagttcgtcc tcggtggtga
       61 cgatggccac ggtgggcgcg gtgatccgcc cgacgaagcc cgtgccgtcg tagcggccga
      121 gctccctccc ggcttcggcg atgtcctcgg ccgtgccgcg ggccagctcg cccaggaacc
      181 aggggatcag cgccacgatc tccggcgggg tgtcgtcctt cagcagcgtg gtgatgcgca
      241 tcggcagcct gccgagcgcc tgcgcccgca ccaggcgctc gacggtgtgg cgggggagca
      301 gccgcaggat gagctggagc gcgcccatgg tccgccacag cgcacgatcc ctcgcggtct
      361 ggctgaacgt gtcgctggtg gcgcacagca ccacgccgcg caccaactcc gcgtggcgca
      421 cggccaggag ctgcgcgatc gggccgccca tggagtagcc gaccgccacc accggcccgg
      481 ttcccagatg gcggaccagg gccgcggcgt cgtcggcggc gtcggccagg cggaacggct
      541 tggatggccg gatgccgtgc ccgtggccgc ggtggtccag ggcgatcacc cgcccgagcg
      601 ccgacagcgg ctcgtagctg gggaaccagt tgaggtctga gggaaacatc cagccgtgca
      661 acagcaggat ggtgggcgcg ccccccggtg cgtgctcggt ggggccggtg tccctgacga
      721 acagctcgcc cctgcccggg acggccacga ggcgcccttc gggcaggccc ggcggtggta
      781 cgggtggggc ggcgtcgggc agctcgggcc ggctccgggc ggcggcccac gcgaccgccg
      841 cggcacccag gccggctcca cccaggacga ggcgcgtggc tctgctcatg ccgcgcacct
      901 taccgccgac aggcctgtgg aaagctctcc caagctctcc tcggggggct tgcgtgattg
      961 tgcgccgccg cgttatagtg cattcgtgaa atcgggctga gcctcgttcg ccctcgctcg
     1021 cttttccggc ggaggcagac cgccgcgagc aggacgagac gccgctcggc aagcacagcc
     1081 cggcaaaccc accgcgcgac ggcgttcccg ccgcgcgcgc gggctgcgcg ggcagaggac
     1141 acgacgtgta ccggaggaag agatgaaggc gctcgtagga gcctgggaca gccggccgca
     1201 gcatgtgctg gcggaactcg ccgcgctgcg tggacggatc aagcagctcg agcaggagct
     1261 ggcccggacc cgcgaggaga acgccgccct gcggtccgag cgcgaggacg gcatggacat
     1321 cgagatcgcc ctgacgccgg cctccgcctc gcgctaggac acggcgcagc accagcgaag
     1381 ccgcccggcc gggcggcttc cgcgtttccg ggaggcgtcc gccgcccggg cgcgccaggg
     1441 tgctttgtcc gcccggcacc ccccgataca ctcgccgcgc ggcccttccc cgtccgccgg
     1501 cgctgatcgc cggcggcccg gccgctcgtg aggccacgag cccgaccccg ccgccgaggt
     1561 gggccggaga tcagactgga aggcacgcgc cgggtgttcc tcaaatcgct cacgctccgc
     1621 ggcttcaagt ctttcgccga caagacgacg ctggagttcg aacccggcat cacggtgatc
     1681 gtgggtccca acggctccgg caagtccaac atcgtggacg ccttcgcctg ggtcttcggc
     1741 acgcacagcg ccaagagcct gcgcggcggg acgatggccg acgtcatctt cgccggcgcg
     1801 cccggcaagc cggcgctggg ccgggcctcc gtcgagctga ccatcgacaa ctccgccggg
     1861 cagctgccga tcgagttcag cgaggtcacg atctcgcggt cgatgttctc ctccggcgag
     1921 aacgagtacc gaatcaacaa cgtggcctgc cggctgctgg acgtgcagga gctgctcagc
     1981 gacaccggcc tgggccgcga gaaccacacg atcgtcgggc agggccagct cgacgccgtg
     2041 ctcaacgcca agccggagga gcgccgggcc ttcatcgagg aggccgcggg catcctcaag
     2101 caccgccgcc gcaaggagcg ggcactgcgc aagctgtcgc agatggagca gcacctggag
     2161 cgcctgaacg acgtcctgcg cgagctgcgg cgcaacctgc gtcctctgga gcgccaggcc
     2221 gaggcggcgg ccaagcacgc gcagctgcag gagcagctca aggccgtgcg cgtggaccgc
     2281 gcgctgcgcg agctcgcggt gctcgacgcg cgctggacca ccgagtccgg cgagcagacc
     2341 cgctccgacg agcgcctcgc cgtgctggag ggcgacctgg cgaccgggcg cagccgcgag
     2401 agcgagctcg accaggcgct gggcgcgctc gcgcccgccg tgcgggaggc ggggcagacc
     2461 cacttccgcc tggccaacct ggtggagcgc taccgcggcc tgtccgggcg catcgaggag
     2521 cgccgcgccg gactgctcga ggcggtcgaa gagccggtcg ccggacgcga cccggaggag
     2581 ctgcgcgccc gcgcgcgcgg cgagcgcggt gcgctggaag agctggaagc ggagcgctcc
     2641 ggggcccaca gcgcgctgga gcaggccgag gaggcgcgcc gcgaggcgga gcgcgcccgc
     2701 cgggcccacg agcaggcggc cgcggccgag gcgcggcgcc gcgccgaggt gcgcgagcac
     2761 cgcctgcgct gggagggcga ggtctccgcg ctgcgcagct cgctggcgca ggcgtccggg
     2821 gaggaggggc ggctggcgtc ccaggtgtcc ggtgtcgagt cccgcaaggc ggagctggag
     2881 cgcgacgtcg agacggtgac ccgcgagatc cagcgcctgg actccgacgc cgcggtgctc
     2941 gccgagcagc ttggcgcgtc cgagcaggtg ctggagcgcc gtcacgagca gcagaaggcc
     3001 gccgcgctgc gcgagcggga gctggagcgc cagcgcgcct cgctggaggc ccgggccgac
     3061 gcgctgcgcg ccgcctcgca ggaggccacc gagggctccg ccgcgctgct cgcgggcgcc
     3121 gagcgcggcg aggtgtccgg cgtcgtcggc gcgctcgcgg accaggtgtc cgtggccgag
     3181 ccgtcgctgg ccaaggccgt cgccgcggcg ctgggcccgc tgggcgacgc gctggtggtg
     3241 cgcgaccgcc gcagcgcgcg cgacgcggtc ggcttcgtgc gctcgcgcca gtccggccgc
     3301 gtgctcctgc tcgtcggcga cggggcgcag cctcccgccg aggacgccga ggcggtcgcg
     3361 gagctggcga tgctcggcgc gcgcccggtc gccgacgtgc tgcgcgccga gccgtccctg
     3421 cacgccgcgc tgcgccaggc gctggccggc gtctacgtgg tcgacgcgac ggacgacggc
     3481 tggggcgctg tctgcaaggt cgcggagcgc tatcccgcgc tggtgttcgt gtccgccgac
     3541 ggcgacgtcg cgggcccgcg cgggtacgcc gggggctcgg cgaccgccaa cagcgccgtg
     3601 ctgtccaagg ccgcggccga gcaggccgag gcgaagctgg cggaggtctc cgacgagctg
     3661 ctgcgggcgc accgccagct cgccgacgcg gaccgcgagc tggaggccgc caagcgcgag
     3721 tacgacgcgg ccgtcgccgc catgcaggag tccgacgggc aggtcaccgc cgccgccgag
     3781 cgcctcaagc gcctgcgcaa ggagctggag acctgcgagc gcgagctggg cgtgctgcac
     3841 ggccagcagg aggacctgtc gcgcgagatg ggccgccagc gcgagcgcct ggcggacctg
     3901 gagtcacggg gcgtggacgc cctgccggag ccggagcccg acgggccgga cctggaggcc
     3961 gaacgcctgg acgacctgct cgccgaggcg cgtgagcgcg aggtgcaggc gcgcctggag
     4021 gtgtccgccg tcgagcagcg cgccggcgag gtggcgcgcc gcatcgacgc ctacgaacgc
     4081 gaggccgccg aggtggaaca ccagctcgtg gagcgcgagc ggcggcgggt ggcccgactg
     4141 gcggccatcg agcgctgcgg cgagctgctc gtgctcgccg gcctggcgct ggcgcgcagc
     4201 gagcagtcgc tggccctggc cgagtccgag cgcgaccggc tggaggaggc gcgtgccgcc
     4261 cagcagcgcg agctcggcgt ggtgcgcgcg cggatccgcg agctggagga ggagctcggc
     4321 gtgctgcgcg aggcacgcca cgcc
//
LOCUS       DATMKC010000430         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_38657, whole genome shotgun sequence.
ACCESSION   DATMKC010000430 DATMKC010000000
VERSION     DATMKC010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38657"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1002..>2598)
                     /locus_tag="VNU01_12480"
     CDS             complement(1002..>2598)
                     /locus_tag="VNU01_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HWH33478.1"
                     /translation="EAQRPGMAIGQQGVTLNRIRAEVGWNPKVVRTPPIPSRTVREVR
                     TYLQSIADERRKFLRRVGRRMHRSVSEGDKWVRMTALGAWREVGRSCSMLQTQDSKVM
                     IDCGVKFEENDPATPMFHLGEVQPFTSLDAVVLTHGHLDHCGLVPLLFKYGYDGPVYC
                     TPPTRDIMALLTMDYIRVAEAQGARKPYDAEHVRAALRNTIAVDWGETTDIAPDVRLT
                     FHDAGHILGSSVCHFHVGEGLYNVAFSGDIKYERSWLFNPAHNKFPRLDAVVVESTYG
                     GKDNAQPSRIEAAEQTRDAVKRTLDRGGKVVFPVFAVGRSQEVMLVLEELMRTGQVPR
                     VPVYLDGMIWEATAIHTCYPEYLDDQLKQKVFQQNENPLLSPIFKRIDSHETRQKVCA
                     SSEPCIVLATSGMLTGGPVIEYMKHWADDPKNTLCFVGFNAEGSLGRKLQRGYREMPV
                     QETGTTRSIRVEMELVTIDGFSGHCDRRQLMNFIQNMEPKPSLVLCGHGEASKCLDLA
                     ASIQQKFEIEARALQNAETIRFR"
BASE COUNT          320 a          929 c          914 g          435 t
ORIGIN      
        1 cggcctcgac gctcgcccgc cacgccttgg ccatcctgct gctcgccggc gcggctggcg
       61 cggcaggcgc cctggccctg cgccgctccg tttccctgcc ggcgccggca gcgcagcgta
      121 cgccgcgacg gcggcgacgg ctgccgccag cacgacgcgg cggagggcgg ccccggaagc
      181 cagcgccacg ggcgagcatg cccctcgtgc acttcaaggg ctcccccgcg acagtgcgga
      241 aggtgccgct ttcgcggcgc cgaactgccg agccgacggc gccttcgcgg gcggcctgac
      301 gcatggcggc cgagcgccgc tggcagattg cttgcctccg ggacctgacc ccatggatgt
      361 ccggcgcgca ccttggcgct gccgcctcca cgggcacccc gttgcgacaa gaccgcgcga
      421 cgaggggcac cgccggccgc cgccgcggcg actggatgcc gaggcccgtg ccgccaaggc
      481 caccgcgtcg cgtcaatggg ccggcggcgg gcaccgcgcg ggcagggcgt gccgccgagt
      541 gcctataggt ccgccgcctc gcggccttcg gccgctcagc gggacctgat ggtctcgacg
      601 ttccgacgcg tcatgcttgg cgcatgccgc gtcgtcccac gtcgcccgtc ggcggcgctg
      661 tggcgcgccg tgggcgtgtc gccgagaccg ataggcccgc cggttcgcgc ttcgcgctca
      721 ccggaacctg atggtctcgg cgctccgacg cgtcatgctt ggcgcatgcc gcgtcgtccc
      781 acgtcgcccg tcggcggggc tgtggcgcat cgtgggcgtg tcgccgagac cgataggccc
      841 gccggttcgc gcttcgcgct caccggaacc tgatggtctc gacgctccga cgcgtcatgc
      901 ttggcgcatg ccgcgtcgtc ccacgtcgcc cgtcggcggc gctgtggcgc atcgtgggcg
      961 tgtcgccgag accgataggc ccgccggttc gcgcttcgcg ctcaccggaa cctgatggtc
     1021 tccgcgttct gcaacgcccg agcctcgatc tcgaacttct gctggatgct tgcagcaagg
     1081 tcgaggcact tgctggcctc gccgtggccg cacaagacaa ggctcggctt cggctccatg
     1141 ttctggatga agttcatgag ctgccggcgg tcgcagtggc cggagaatcc gtcgatggtg
     1201 acgagctcca tctccacgcg gatgctgcgc gtcgtccccg tctcctgcac cggcatctcc
     1261 cggtagcccc gctggagctt ccggcccagg ctgccttccg cgttgaaccc cacgaagcag
     1321 agcgtgttct tcgggtcgtc cgcccagtgc ttcatgtact cgatgacggg gccgccggtg
     1381 agcatgccgc ttgtggcgag cacgatgcag ggctcgctgg acgcgcagac cttctggcgc
     1441 gtctcgtggc tgtcgatgcg cttgaagatg ggtgacagca gcgggttctc gttctgctgg
     1501 aagaccttct gcttcagctg gtcgtccagg tactccgggt agcaggtgtg gatggcggtg
     1561 gcttcccaga tcatcccgtc caggtacacc ggcacgcgcg gcacctgccc ggtgcgcatg
     1621 agctcctcca gcaccagcat cacctcctgg ctgcggccga cggcgaacac ggggaagacg
     1681 accttgccgc cgcggtcgag ggtccgcttg acggcgtccc gcgtctgctc cgccgcctcg
     1741 atgcgcgacg gctgcgcgtt gtccttgccg ccgtacgtgc tctcgacgac cacggcgtcg
     1801 aggcgcggga acttgttgtg ggccgggttg aacagccagc tgcgctcgta cttgatgtcg
     1861 cccgagaacg ccacgttgta cagcccctcc ccgacgtgga agtggcagac gctggagccc
     1921 aggatgtggc cggcgtcatg gaacgtgagg cgcacgtcgg gggcgatgtc cgtcgtctcg
     1981 ccccagtcga cggcgatggt gttgcggagg gccgcgcgca cgtgctccgc gtcgtacggc
     2041 ttgcgggcgc cctgcgcctc cgcgacgcgg atgtagtcca tcgtgaggag ggccatgatg
     2101 tcgcgcgtgg gcggcgtgca gtacacgggg ccgtcgtagc cgtacttgaa cagcagcggc
     2161 acgaggccgc aatggtccag gtggccgtgc gtgagcacca cggcgtcgag gctcgtgaac
     2221 ggctgcacct cgccgaggtg gaacatcggg gtggcggggt cgttctcctc gaacttgacg
     2281 ccgcagtcga tcatgacctt cgagtcctgg gtctggagca tgctgcagga gcggccgacc
     2341 tcgcgccaag cgccgagcgc cgtcatgcgc acccacttgt cgccctcgct gacgctgcgg
     2401 tgcatgcgcc gcccgacgcg gcggaggaac ttccggcgct cgtccgcgat gctctgcagg
     2461 tacgtccgca cttcgcgcac cgttcggctc gggatgggcg gcgtgcgcac gaccttcggg
     2521 ttccagccga cctcggcgcg gatgcggttc agcgtgaccc cctgctggcc gatcgccatg
     2581 ccgggccgct gcgcctcc
//
LOCUS       DATMKC010000431         9077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_36785, whole genome shotgun sequence.
ACCESSION   DATMKC010000431 DATMKC010000000
VERSION     DATMKC010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36785"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VNU01_12485"
     CDS             complement(<1..187)
                     /locus_tag="VNU01_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33479.1"
                     /translation="MAERPFRPVEILRVLLEHEVQFVVIGGYGAYLQGSPVPTLDVDV
                     TPSARRDNLERLASALRD"
     gene            complement(180..494)
                     /locus_tag="VNU01_12490"
     CDS             complement(180..494)
                     /locus_tag="VNU01_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWH33480.1"
                     /translation="MRGGLLIRQARRRGRLTQTQLAERLGSTQSAIARWESGKAEPSF
                     HSVVRAVRACGFDFVPQLLPRDESEERMIDYHLSLTTMQRAAALEELLRVGEQARTTP
                     RG"
     gene            complement(539..1429)
                     /gene="era"
                     /locus_tag="VNU01_12495"
     CDS             complement(539..1429)
                     /gene="era"
                     /locus_tag="VNU01_12495"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWH33481.1"
                     /translation="MRSGFCSIVGRPNVGKSTLLNAVVGQKVAITTPVPGTTRHAIRG
                     ILHAQRDGEPAQVVFVDTPGLHKPRTMLGNRMNDTAREALSGVDCVVFMVDASQAIGR
                     GDEFVASLLADVKAPKLAVLNKVDLVAKPKLLPQLTRVAELGDWDEIVPISAAGRDGV
                     ELLAGLIVARMPEGPPYFPEGQVSDQPVELALAEIIREKAITIMREEVPHSIAVLVEE
                     ILPGEREDVRVVHANLYVERDSQKGIVIGKGGEVLKSIGARARAELELLLGARVYLEL
                     RVKLMKEWQRDAKALARLGY"
     gene            complement(1454..2185)
                     /locus_tag="VNU01_12500"
     CDS             complement(1454..2185)
                     /locus_tag="VNU01_12500"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWH33482.1"
                     /translation="MELALVALAVVAGALVKSVTGLGLPLLTIPVLAQVVGVREAVVV
                     MAPPTLLANAWMTWRYRAALGMVPNLWRLLAASVAGGIAGAALLERVNERALALVLAV
                     AVLGYVVRLWRDPDVRLADRTARRLALPLGLFAGGLQGATGISGPVVSTYVHAQRLPR
                     EQFVVAVSALFGVSGMAQTATLAGVGLFTPALLLRAGLATAVVAAVMAVGTRVADRLP
                     QALFERLVLAVLVVSGSKLLWDALA"
     gene            complement(2192..2623)
                     /gene="cdd"
                     /locus_tag="VNU01_12505"
     CDS             complement(2192..2623)
                     /gene="cdd"
                     /locus_tag="VNU01_12505"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWH33483.1"
                     /translation="MTGVDWDALLEQAYAAQELAYAPYSGFRVGCALLAADGRVFVGA
                     NIENAAYSPTICAERAALPAAVVAGATEFAALAVAGDGPGPCTPCGVCRQVLYEFAPD
                     LEILAAGSDRASARYVLSRDLLPHGFGPARLAKGAGPGARG"
     gene            complement(2620..3909)
                     /locus_tag="VNU01_12510"
     CDS             complement(2620..3909)
                     /locus_tag="VNU01_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWH33484.1"
                     /translation="MTPGDTWTVAGSALLVLLAAVFSAAQAALSRMTVHRAAKLVEDG
                     GRRARTLLRLVEHPVRTLNILALAVLLLQVTAVALVAVVADRHLPDAAAVALTVAAAG
                     MGMFVVAEAAPKTLALQNTERVALMTAPLVAVFVRPLAPLARLLIGVANVVVPGPAPA
                     GGPFVVTEDELREMIDVAEDTEIIEESEREMIHSIFELGDTVVREIMVPRPDMATVSD
                     ADSLSDVLESMLASGHSRLPVYHGDRDHIVGLVYAKDVLRRLHATASETGPWQDLLRS
                     AHFVPELKPVDALLRELQAEKVHMAIVVDEYGATAGLVTIEDILEEIVGEIVDEYDTE
                     EILVEALDENAWVVDARLPVDDLSKLVDSELPSEGWDTVGGLLFGMLGHVPSPGERVD
                     VDSVRLVAERVKGRRISKVLVRRMPVPDPPAVGQDAR"
     gene            complement(3906..4391)
                     /gene="ybeY"
                     /locus_tag="VNU01_12515"
     CDS             complement(3906..4391)
                     /gene="ybeY"
                     /locus_tag="VNU01_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWH33485.1"
                     /translation="MAVFVADEQAGSGAAPDVDVERLQRLARHVLADRRVPDGMELSV
                     LCVDADDMAELNGHHMGKQGPTDVLAFPMDLPGETLPGEPAILGDVVLCPAVAAAQAA
                     EHGKTFAAELELLLVHGILHLLGHDHAEPAERAEMFGLTDRLLASFAAEPSTAERGEH
                     P"
     gene            complement(4396..5370)
                     /locus_tag="VNU01_12520"
     CDS             complement(4396..5370)
                     /locus_tag="VNU01_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWH33486.1"
                     /translation="MSAPAQVKTSVPGHHNMVSLLGTRDELLKMVEKAFDVDILVRGN
                     DITVTGPEEEGRKVVAVFEEFVKLLDKGQGLDEGNVSATIAMIQGAEHPSPSEVLSDE
                     ALHHRGKVIRPKTLGQKRYLDAIRQNTVVFGIGPAGTGKTYLAMAMAVLALKRKEVNR
                     IILTRPAVEAGERLGFLPGTLAEKIDPYLRPLFDALHDMMDAEQLTLLMERGTIEVAP
                     LAYMRGRTLNDSFIVLDEAQNTTPEQMKMFLTRLGFGSKAVVTGDVTQVDLPSGKHSG
                     LRLVRDILTDIEGVEFCELGARDVVRHRIVQDIVEAYSAWDSSRERED"
     gene            complement(5441..5848)
                     /locus_tag="VNU01_12525"
     CDS             complement(5441..5848)
                     /locus_tag="VNU01_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33487.1"
                     /translation="MAKVKLAQIAIARSGDKGDRSDLGLFAPAGPHIDEVYAVLEREV
                     TAERVKALFGDFVLGEVTRYAAPNVHGLKFVMEQALGGGGPRSLRADNLGKALGGALL
                     RLEVEVPDELLAKLGPRPAPPRDPYASAAWAVR"
     gene            complement(5914..7260)
                     /locus_tag="VNU01_12530"
     CDS             complement(5914..7260)
                     /locus_tag="VNU01_12530"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HWH33488.1"
                     /translation="MIERPDGAVRIAGGQGFYGDAPGPLADLLTEQPDYVVLEALAEL
                     TLAILNKDRARDESLGYTRDLPLYLLQLLPAVIDGKTRVITNAGGINPHAAAQMITKA
                     ADRFGLKGFTVATVVGDDVLGRPALLPEDFPSAEALAFANAYLGARPLVEALEAGADL
                     VLTGRVADAALFLAPLVHEHGWAWDDWDKLAAGMLVGHLLECSGQSTGGNHAGRWWEV
                     PEPWRFGFPLADVAADGTATIFKPEVAGGRVDYDTVRQQLFYEVHDPGNYLTPDVVVD
                     MTTLTLEDLGGDRVRVTGVTGKPATPTYKVVGAVAEGYAADLSIAFGWPDAEAKARAA
                     ADIARKRIEVAGLELDDWHVELFGVNALHGPAVNLDEVDQPSEVVVRMAWRAADRDVV
                     DQVPRHVVPLGLSAPPAGFTPAGRSRPKSSALLRIHTGLVDKRAVDEGVRVQVEHV"
     gene            complement(7257..8891)
                     /locus_tag="VNU01_12535"
     CDS             complement(7257..8891)
                     /locus_tag="VNU01_12535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HWH33489.1"
                     /translation="MAELASGVLAMRALKAAGVEHLFTLSGGHIFPLYDGCRDAGIRI
                     VDTRHEQTATFAAEGMAKLTRRTQVAALTAGPGITNGASAVAGAKFNGSPVLVLGGRA
                     PQATWGAGSLQEMDHVAFMRALTKHAMTSTAPGEVARDVLDAFRVASTPQRGPAFVDI
                     PMDVIFLPADEATVPGWAPPEPQPVDTDAVARAAEVLRGARKPVIIAGTDVWFDGAWQ
                     ALRDLVETLRVPTILNGMGRGCLPADHELCFSRARGAALKGADVVMVIGTPLDFRLGF
                     GHFGEAQVVHVVAHPDLVARHRPLAAGVSGNLAAALAEITQGVEGPAGETQAARDAWV
                     AELRGVEDAERASERDLLESEADPIHPARIYGELGKALERDAVVIGDGGDFVSYAGKY
                     VDTYEPGRWMDPGPYGCLGTGPGYAAAARLAHPDAQVALMLGDGAAGFSLMDVDTLVR
                     HNLPVVMVVGNNGMWALEKYPMQQMYAGWDVAADLRQETGYDAIVRALGGAGETVDKA
                     GDLAAALKRGFDSGVPYLINVHTDPKVAYPRKAALV"
BASE COUNT         1258 a         3387 c         3169 g         1263 t
ORIGIN      
        1 gatcgcgcag cgccgacgcg agccgctcaa ggttgtcacg gcgtgcgctg ggggtcacgt
       61 cgacatcgag cgtcggcacc ggcgagccct gcaggtaggc gccatagccg ccgatgacga
      121 cgaactgcac ctcgtgctcc agcagcacgc ggaggatctc gacgggacgg aagggccgct
      181 cagccacgcg gcgtcgtccg tgcctgctcg ccgacacgca acagctcttc caaagccgcc
      241 gcgcgctgca tggtcgtgag actcaagtgg tagtcgatca tgcgctcctc cgactcgtcg
      301 cgaggcagca actgtggcac gaagtcgaag ccgcatgccc ggacggcgcg cacgacgctg
      361 tggaaagacg gctccgcctt ccctgactcc caccgcgcga tcgctgactg cgtggagccg
      421 agccgctccg cgagctgtgt ctgcgtcagc cggccacgcc gccgcgcctg cctgatgagc
      481 aatcctccgc gcatactcat agcataagag ctatatagct gttctgctat gcatatttct
      541 agtagccgag gcgggcgagg gccttcgcgt cgcgctgcca ctccttcatg agcttcacgc
      601 gcagctccag gtacacgcgc gcgccgagca gcagctccag ctcggcgcgg gcacgcgcgc
      661 cgatggactt gagcacttcg ccgcccttgc cgatgacgat gcccttctgg ctgtcgcgct
      721 ccacgtagag gttggcgtgc accacgcgga cgtcctcgcg ctcccccggc aggatctcct
      781 cgaccagcac ggcgatggag tggggcacct cctcgcgcat gatcgtgatg gccttctcgc
      841 ggatgatctc ggccagcgcc agctcgaccg gctggtcgct gacctgcccc tccgggaagt
      901 acggcggccc ctccggcatc cgggcgacga tgaggccggc gagcagctcc acgccatcgc
      961 ggccggccgc cgagatcggc acgatctcgt cccagtcccc cagctcggca acgcgcgtga
     1021 gctgcggcag gagcttgggc ttggcgacca ggtccacctt gttgagcacc gcgagcttgg
     1081 gcgccttgac gtcggcgagc agcgaggcca cgaactcgtc gccccggccg atggcctgcg
     1141 aggcgtccac catgaacacc acgcagtcca ccccggacag ggcctcccgc gcggtgtcgt
     1201 tcatgcggtt gccgagcatc gtgcgcggct tgtgcagccc gggcgtgtcc acgaacacca
     1261 cctgcgcggg ctcgccatcg cgctgcgcat ggaggatccc gcggatggcg tggcgcgtgg
     1321 tgccgggcac cggcgtggtg atcgccacct tctgccccac cacggcgttg agcagggtcg
     1381 acttgcccac gttggggcgg ccgacgatgg agcagaaacc cgagcgcatg gcgcgaaggc
     1441 tacccgcgcg ccgtcaggcc agcgcatccc acagcagctt gctgcccgac acgaccagca
     1501 ccgcgagcac gaggcgctcg aacagcgcct gagggaggcg gtcggcgacg cgcgtgccga
     1561 ccgccatcac cgcggccacc acggcggtcg ccaacccggc gcgcaggagc agcgcaggag
     1621 tgaacagtcc gacaccggcc agcgtggcgg tctgcgccat gccggacacg ccgaacagtg
     1681 cgctgacggc caccacgaac tgctcgcgcg gaagccgctg ggcatgcacg taggtgctga
     1741 ccaccgggcc ggagatgccc gtggcgccct gcagcccacc ggcgaagagc cccagcggca
     1801 gcgcgagccg gcgtgcggtg cggtcggcca gccgcacatc gggatcacgc cagaggcgca
     1861 ccacgtagcc cagcaccgcg acggcgagga ccagggcgag cgcgcgctcg ttcacgcgtt
     1921 ccagcagcgc cgccccggcg atgccgccgg ccacgctcgc ggcgagcagc cgccagaggt
     1981 tcggcaccat gcccagcgcg gcccggtagc gccatgtcat ccaggcgttg gcgagcagcg
     2041 tgggcggcgc catgacgacg accgcttcgc gcacacccac gacctgcgcc aggacgggga
     2101 tggtgagcag cggcagcccc agaccggtga cgctcttgac cagcgcgccc gcgacgaccg
     2161 ccagcgccac cagcgccagc tccacgccgg ctcagccccg cgcgccgggg ccggcgccct
     2221 tcgccaggcg cgccgggccg aacccgtgcg gcagcaggtc gcgcgacagc acgtaccgcg
     2281 cggacgcccg gtcgctgccc gccgccagga tctccaggtc gggcgcgaac tcgtacagca
     2341 cctgccggca caccccgcaa ggggtgcacg ggcccggccc gtcgccggcc actgccaggg
     2401 cggcgaactc cgtggcgccc gcgacgacgg ccgccggcag cgcggcacgc tcggcgcaga
     2461 tcgtgggcga gtaggcggcg ttctcgatgt tggcgccaac gaacacgcgg ccgtccgcgg
     2521 ccagcagcgc gcagccgacg cggaagccgg agtacggcgc gtaggcgagc tcctgggcgg
     2581 cgtaggcctg ctccagcagc gcgtcccagt cgacgcccgt caccgcgcgt cctgtcccac
     2641 ggcgggaggg tcgggcaccg gcatcctgcg gaccagcacc ttgctgatgc gccggccctt
     2701 cacgcgctcg gccacgagcc gcacggagtc gacgtcgacg cgctcgcccg gggacggcac
     2761 gtgcccgagc atgccgaaca gcagcccgcc caccgtgtcc cagccctcgc tgggcagctc
     2821 gctgtcgacc agcttggaca ggtcgtcgac tggcagccgc gcgtccacca cccacgcgtt
     2881 ctcgtccagc gcctcgacca ggatctcctc ggtgtcgtac tcgtcgacga tctcgccgac
     2941 gatctcctcc aggatgtcct cgatggtgac caggcccgcc gtggcgccgt actcgtccac
     3001 cacgatcgcc atgtgcacct tctccgcctg cagctcccgc agcagcgcgt ccaccggctt
     3061 gagctcgggc acgaagtgcg ccgagcgcag caggtcctgc cacggcccgg tctcgctggc
     3121 ggtggcgtgc aggcggcgca gcacgtcctt ggcgtagacc agcccgacga tgtggtcgcg
     3181 gtcgccgtgg tagacgggca agcgcgagtg gccactggcg agcatggact ccagcacgtc
     3241 gctgagcgag tcggcgtcgg agaccgtcgc catgtcgggc cggggcacca tgatctcgcg
     3301 caccaccgtg tcgccgagct cgaagatcga gtggatcatc tcgcgctcgg actcctcgat
     3361 gatctcggtg tcctccgcga cgtcgatcat ctcgcgcagc tcgtcctcgg tgaccacgaa
     3421 cggcccgccg gccggcgccg gtcccggcac cacgacgttg gccacgccga tcagcagccg
     3481 cgccaggggc gccagcggcc gcacgaacac ggcgaccagc ggcgccgtca tgagcgccac
     3541 gcgctcggtg ttctgcaggg ccagcgtctt gggcgcggct tccgccacca cgaacatgcc
     3601 catgcccgcg gcggccaccg tgagcgccac cgcggcggcg tccggcaggt gccggtctgc
     3661 caccacggcg accagggcga cggccgtgac ctgcagcagc aggaccgcca gcgccaggat
     3721 gttcagcgtg cgcaccgggt gctcgaccag gcgcagcagg gtgcgcgccc gccggccgcc
     3781 gtcctcgacc agcttcgcgg cgcggtggac ggtcatgcgc gacagggcgg cctgggccgc
     3841 ggagaagacc gcggcgagca acaccagcag tgccgagccc gccacggtcc aggtatcgcc
     3901 aggtgtcatg ggtgctcgcc tcgctcggcg gtcgacggct ccgcggcgaa gcttgccaga
     3961 aggcggtcgg tgaggccgaa catctccgcc cgctccgccg gctcggcgtg gtcgtgcccc
     4021 agcaggtgca ggatgccgtg cacgagcagc agctccagct cggccgcgaa cgtcttgccg
     4081 tgctcggcgg cctgcgcggc ggcgacggcc gggcacagca ccacgtcgcc gaggatggcc
     4141 ggctcgcccg gcagcgtctc ccccggcagg tccatcggga aggcgagcac gtccgtgggg
     4201 ccctgcttgc ccatgtggtg gccgttcagc tccgccatgt cgtcggcgtc gacgcacagc
     4261 acggacagct ccatgccgtc cgggacgcgc cggtcggcca ggacgtggcg ggccagccgc
     4321 tgcaggcgct cgacatccac gtcgggcgcc gcgccggacc cggcctgctc gtcggccacg
     4381 aagacggcca tcgcgtcagt cttcccgctc gcgggaggag tcccaggccg agtacgcctc
     4441 gacgatgtcc tgcacgatgc ggtggcgcac gacgtcgcgc gcgcccagct cgcagaactc
     4501 cacgccctcg atgtcggtga ggatgtcgcg caccaggcgc aggccgctgt gcttgccgct
     4561 gggcaggtcc acctgcgtga cgtcgccggt gaccaccgcc ttggagccga agcccagacg
     4621 ggtcaggaac atcttcatct gctccggcgt ggtgttctgc gcctcgtcca gcacgatgaa
     4681 gctgtcattc agagttctac cacgcatata ggccaggggg gcgacctcga tggtgccgcg
     4741 ctccatcagc agggtgagct gctcggcgtc catcatgtcg tgcagggcgt cgaacagcgg
     4801 gcgcaggtac gggtcgatct tctccgcgag cgtgcccggc aggaagccga gccgctcccc
     4861 cgcctccacg gccggccgcg tgaggatgat gcggttgacc tccttgcgct tgagcgccag
     4921 gacggccatc gccatcgcca ggtaggtctt gccggtgccc gcggggccga tgccgaacac
     4981 caccgtgttc tgccggatcg cgtccaggta gcgcttctgc cccagcgtct tgggacggat
     5041 caccttgccg cggtggtgca gcgcctcgtc gctcagcacc tccgaggggc tggggtgctc
     5101 cgctccctgg atcatggcga tggtggcgct gacgttgccc tcgtccagcc cctggccctt
     5161 gtccaggagc ttcacgaact cctcgaagac ggccacgacc ttgcggccct cttcctcggg
     5221 gccggtgacc gtgatgtcgt tgccgcgcac caggatgtcg acgtcgaagg ccttctcgac
     5281 catcttgagc agctcgtcgc gcgtgccgag cagcgacacc atgttgtggt ggccggggac
     5341 ggacgtcttg acctgtgcgg gtgcgctcaa gaagaggctc tggctgtcgg cgacgggaac
     5401 gcccgggatt ttacctgccg cgccgtccgc tcacaccggc tcaccgcacg gcccaggccg
     5461 cggaggcgta ggggtcgcgc ggcggcgccg gccgggggcc gagcttggcc agcagctcgt
     5521 ccggcacctc gacctccagg cgcagcagcg cgccgcccag cgccttgccc aggttgtccg
     5581 cgcgcagcga gcgcggcccg ccgccgccca gcgcctgctc catcacgaac ttcagcccat
     5641 gcacgttcgg tgcggcgtag cgggtgacct cgcccaggac gaagtcgccg aacagcgcct
     5701 tcacgcgctc ggcggtgacc tcgcgctcca gcacggcgta gacctcgtcg atgtgcggcc
     5761 cggcgggcgc gaacagcccc aggtcgctgc ggtcgccctt gtcgcccgac cgtgcgatcg
     5821 cgatctgcgc cagcttcacc ttggccatgc ctgccgcccc cactctccca acctggaacg
     5881 ttcgtccggt atctcggacg aacgttcaat gtgtcagacg tgttcgacct gcacgcgcac
     5941 gccctcgtcg acggcccgct tgtccaccag ccctgtgtgg atgcgcagca gggcactgga
     6001 cttggggcgc gaacgcccgg cgggcgtgaa gcccgcgggc ggcgcggaca atcccagcgg
     6061 cacgacgtgg cgcggcacct ggtcgacgac gtcgcggtcg gccgcgcgcc acgccatgcg
     6121 caccaccacc tcgctcggct ggtccacctc gtccaggttc acggccggcc cgtgcagcgc
     6181 gttcacgccg aacagctcca cgtgccagtc gtccagctcc aggccggcca cctcgatgcg
     6241 cttgcgggcg atgtcggcgg cggcgcgcgc ctttgcctcc gcgtccggcc agccgaacgc
     6301 gatggacagg tccgcggcgt agccctcggc gaccgcgccg acgaccttgt aggtcggcgt
     6361 cgcgggcttg ccggtcacgc cggtgacgcg gacccggtcg ccgcccaggt cctccagcgt
     6421 cagcgtggtc atgtccacca ccacgtccgg cgtcaggtag ttgcccgggt cgtgcacctc
     6481 gtagaacagc tgctggcgca ccgtgtcgta gtccacgcgg ccgcccgcca cctccggctt
     6541 gaagatggtg gccgtgccgt cggcggcgac gtcggccagg gggaagccga agcgccacgg
     6601 ctccggcacc tcccaccaac ggcccgcgtg gttgccgccg gtggactgcc cgctgcactc
     6661 cagcaggtgg ccgaccagca tgccggcggc gagcttgtcc cagtcgtccc aggcccagcc
     6721 gtgctcgtgg accagcggcg ccaggaacag cgccgcgtcg gccaccctgc cggtgagcac
     6781 caggtcggcc ccggcctcca gcgcctcgac cagcgggcgg gcgcccaggt aggcgttggc
     6841 gaacgccagc gcctcggcac tggggaagtc ctccggcagc agcgccgggc ggcccagcac
     6901 gtcgtcgccg accacggtcg ccacggtgaa gcccttgaga ccgaagcggt cggcggcctt
     6961 ggtgatcatc tgcgcggccg cgtgcgggtt gatgccgccg gcgttggtga tgaccctggt
     7021 cttgccgtcg atcacggcgg gcagcagctg cagcaggtac agcggcaggt cgcgcgtgta
     7081 gcccagtgac tcgtcccgcg cgcggtcctt gttgaggatc gccagggtca gctcggcgag
     7141 cgcctcgagc acgacgtagt cgggctgctc ggtgagcagg tccgcgagcg ggccgggggc
     7201 gtcgccgtag aagccctgtc ccccggcgat gcgcacggcg ccgtcgggtc gctcgatcac
     7261 accagggccg ccttgcgcgg gtaggccacc ttcgggtcgg tgtgcacgtt gatcaggtag
     7321 ggcacgccgc tgtcgaagcc gcgcttgagg gcggcggcca ggtcaccggc cttgtccacc
     7381 gtctcccccg cgccgccgag cgcgcgcacg atcgcgtcgt agccggtctc ctgccgcagg
     7441 tccgccgcca cgtcccagcc ggcgtacatc tgctgcatgg ggtacttctc cagcgcccac
     7501 atgccgttgt tgcccaccac catcaccacc ggcaggttgt ggcgcaccag cgtgtcgacg
     7561 tccatgaggc tgaagcccgc ggcgccgtcg ccgagcatca gcgccacctg cgcgtcggga
     7621 tgagccagcc gcgcggccgc cgcgtagccc ggcccggtgc ccaggcagcc gtacggcccg
     7681 gggtccatcc agcgcccggg ctcgtaggtg tcgacgtact tgccggcgta gctcacgaag
     7741 tccccgccgt cgccgatgac cacggcgtcg cgctccaggg ccttgccgag ctcgccgtag
     7801 atgcgcgccg ggtggatcgg gtcggcctcg gactccagca ggtcgcgctc gctcgcgcgc
     7861 tcggcgtcct cgacgccgcg cagctcggcc acccaggcgt cgcgcgcggc ctgcgtctcg
     7921 ccggccgggc cctcgacgcc ctgggtgatc tcggccagcg cggcggccag gttgccgctg
     7981 acgcccgcgg ccagcggccg gtgcctggcg accaggtccg ggtgcgccac gacgtgcacc
     8041 acctgcgcct cgccgaagtg gccgaagccc aggcggaagt ccagcggcgt gccgatgacc
     8101 atgaccacgt ccgcgccctt gagcgcggcg ccgcgcgcgc gggagaagca cagctcgtgg
     8161 tcggccggca ggcagccgcg gcccatgccg ttgaggatcg tcggcacgcg cagcgtctcg
     8221 acgaggtcgc gcagcgcctg ccaggcaccg tcgaaccaca cgtccgtgcc ggcgatgatc
     8281 accggcttgc gcgcgccccg caggacttcg gccgcccgcg ccaccgcatc ggtgtcgacc
     8341 ggctgcggct ccggcggcgc ccagccgggc acggtggcct cgtcggcggg caggaagatc
     8401 acgtccatcg ggatgtcgac gaacgcgggc ccgcgctgcg gcgtgctcgc cacccggaag
     8461 gcatccagca cgtcgcgcgc gacctcgccc ggagcggtgg aggtcatcgc gtgcttggtg
     8521 agcgcccgca tgaacgcgac gtggtccatc tcctgcagcg acccggcgcc ccacgtggcc
     8581 tgcggcgccc ggccgcccag gaccagcacc ggggagccgt tgaacttggc gcccgcgacg
     8641 gcgctggcgc cgttggtgat gcccgggccg gccgtgagcg cggcgacctg cgtgcggcgg
     8701 gtgagcttgg ccatgccctc ggcggcgaac gtcgcggtct gctcgtgacg ggtgtcgacg
     8761 atgcggatgc cggcgtcgcg gcagccgtcg tacagcggga agatgtggcc gccggacagg
     8821 gtgaacaggt gctccacacc cgcggccttc agcgcccgca tcgccaggac gccgctcgcc
     8881 agctccgcca tggtcgctcc ctctcatcga tcgatcgaac ggacccgcag cctagaccgt
     8941 ggaggccacg ggtcggggtg acagctcggc tctctgtgcg gactctacga gttcggtggg
     9001 ggttcggccg cgcatccagt ttccgtggtt gcggcggcgg gtgttgtagc ggtggagcca
     9061 gccttggagc tggcggt
//
LOCUS       DATMKC010000432        10079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_13625, whole genome shotgun sequence.
ACCESSION   DATMKC010000432 DATMKC010000000
VERSION     DATMKC010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13625"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VNU01_12540"
     CDS             <1..1441
                     /locus_tag="VNU01_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33490.1"
                     /translation="LLGGGDDVLGAALAARLPLARAADDAWSLPAPLGDLIQPAPLEP
                     SEAVAVAEWCLARDLAAEAFAVLWAGGHDEAALGLLERCGGAEAEALLAGWTGPLELP
                     SWRLRDTPVAAERLASAAERAGHPQREPRRRLAAVPDVHGPLAGQRERAQLARDLLQR
                     GHGAEARREAATLLRETTAPDVRARALEIIARVARRAGSAEELSLASTLLREALALWR
                     REDEPGRVAETRLHLAWVDRDRGHSALALERVATVRRGAPPGGALWVQATLAEAALLC
                     DTGRCQAAGALVDELERVAWAWAGPRVKSRAAVVRAWAAVHAEHDEDAARRTEEITAD
                     LLGWMDGVCGLRNLAAAAEIWARLGRHDRVAWCLRLIDTVQVAPDWEVTLARAGAECR
                     IGDPVAAMAMLRPLEPPGAAPGRDRWRVLLLGAFAALRAGAQDAPARAVAALQAAVAA
                     GDPYAPFVRERDLARRVLAAAGPVAGGAG"
     gene            1438..2154
                     /locus_tag="VNU01_12545"
     CDS             1438..2154
                     /locus_tag="VNU01_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWH33491.1"
                     /translation="MRVRVLGRFAVTRPGADVTPTGIQAQLLKALVCGGRPMHWEEVV
                     EVVWPGTGPKRGRIRLRNALSRLRGRCGPVAMRAGAMLMIPPEVGVDAVEFEALAQRA
                     LEAGPSEEGLRLARQALEHYRGDLLPEDRYLDWTMPFRERLRRRFRGLVDLLASDARA
                     RGDLEGALLLLERALADEPEDEGLALDTVELLLALGRSGSAVRLLDRVRAELDQQGVP
                     VSARWGDLRRQARGPEQGAG"
     gene            complement(2151..2909)
                     /locus_tag="VNU01_12550"
     CDS             complement(2151..2909)
                     /locus_tag="VNU01_12550"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWH33492.1"
                     /translation="MAKTRRDALGDGVWLLTLTDPDRRNAMDEAMGAELVDAAAALAA
                     DPAAKALVVTGEGEAFCAGADLPALFGDADRPVEETRARLQRYYRGFLALRELAIPTI
                     AAVNGAAVGAGLNLALACDVRVAGPDAAFGATFARIGLHPGGGCTWFLVQALGPSRAL
                     RTLLLGDTLDAERAVAWGLAEGPEADPVAAAVELARRFAVAEPALARHIKRSVRLAAE
                     HAGLDAVLEYESWAQASSAGSDTLRAWVDRFRKA"
     gene            3059..3658
                     /locus_tag="VNU01_12555"
     CDS             3059..3658
                     /locus_tag="VNU01_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33493.1"
                     /translation="MDSSTPTTAGLERIAPVEPPYDAEVDAELGRWMPPGSPFEPLQV
                     FRVLARNLPLMQRMLPLGSGLLNHPSVSHRDRELIIQRTCALAGAEAEWATHVFAFGK
                     AVGITREQTASLVFGGPADACWTDERDRLVIELCDQLHATATVTPDLRGRLAREFDDG
                     QVVELLVIAGWYRTFSYVVNGAGVTPEEFVPRFTDFPLP"
     gene            3760..4455
                     /locus_tag="VNU01_12560"
     CDS             3760..4455
                     /locus_tag="VNU01_12560"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWH33494.1"
                     /translation="MSTWEELRAQAAACTRCRLASGRTQVVFGVGDPDASLMFVGEAP
                     GFHEDQRGEPFVGRAGKLLDTLCADVGLSRERGVYIANVLKCRPPDNRDPLPDEIAAC
                     KPFLLEQLAHVDPGVVVTLGAFATRPLLADAEPGPPDPSRVSVSKVAGYRFHVHGRTL
                     IPTYHPAAALRGNAAALDALRRDLRLAADVLAGRVPTAAEALGLSAPAEPPAAAPAAD
                     APGGDPALQLRLL"
     gene            4456..4974
                     /gene="tsaE"
                     /locus_tag="VNU01_12565"
     CDS             4456..4974
                     /gene="tsaE"
                     /locus_tag="VNU01_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWH33495.1"
                     /translation="MSECTLRSASPEETRAIGAALAGALRAGDVVSLTGELGAGKTCL
                     VQGAAAALGVAERVTSPSFLLRKDYQGRVPVLHLDVYRLETLRDVMDIGWDEAVDGSR
                     VSFVEWGDAMSPLLPEEYLEVDLRAVNDDQAALGEEPRRLLLRARGEDWARRLAELVP
                     ALRRWRDPGSAC"
     gene            4968..5720
                     /gene="tsaB"
                     /locus_tag="VNU01_12570"
     CDS             4968..5720
                     /gene="tsaB"
                     /locus_tag="VNU01_12570"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWH33496.1"
                     /translation="MLILGIDTCTGRTSVALGSGDGVLASASLARPTAHGGFLAPAIE
                     FCLRQAGAGVGDLHGVAVTLGPGLFTGMRVGIATAQALAHARRLPVVGLASLDLLAFR
                     VRHARRPVCAVLDAKRKELFWAFYRPVPGGVQREGEFRVGPAEKLAAELEASGQDTLC
                     VGDGAVAHRSLLESAGADVDPSPALASPHAESLVELAVPRFLREETQRAEELRPVYLR
                     QADARIGWRERGVLSGGMAGPSGGGPAAGEAG"
     gene            5726..6352
                     /gene="rimI"
                     /locus_tag="VNU01_12575"
     CDS             5726..6352
                     /gene="rimI"
                     /locus_tag="VNU01_12575"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWH33497.1"
                     /translation="MAASRRLALSGVRLEPMHPRHLRQVLAIEQAVYPRPWTPGVFLS
                     ELEAGDSRRYYVALAGRGGRLARLRGPRVVGYGGIMVQVGEAHVTTVAVDPREHRRKV
                     ASRLLVRLLADARAMGAEAATLEVRTTNLGAQRLYQAFGFAPVGVRPGYYAETGEDAL
                     IMWAHELQGERMRQRLEEQAARLDQPGGASGQPDLEVPWVRGRTGLEG"
     gene            6354..7391
                     /gene="tsaD"
                     /locus_tag="VNU01_12580"
     CDS             6354..7391
                     /gene="tsaD"
                     /locus_tag="VNU01_12580"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWH33498.1"
                     /translation="MLVLGIETSCDETAVALVEDGLRVRSNVIGTQVDQHRPYGGVVP
                     EIAARAHLELMLPVLDRALIEAGCTYDDVEGIAVTVGPGLVGALLVGVAAAKSLALAR
                     DVPLIGVNHLEGHICATQLEFGPLALPLVALIVSGGHTSVILLDEDGHSRTLGATIDD
                     AAGEAFDKVARFLGLPYPGGPEIDRLARDGDPAAIAFPRALMHERGYDFSMSGLKTAV
                     VRELRRREAAGEPIHLPDVAASFQEALVDVQVRKTVAAAVDHGVPTVTVVGGVAANSR
                     LRQRMEQACAKAGLRLLVPSPALCTDNGAMIAAAGFNRLAAGERTALSVDADPNLPLR
                     PIPVAPAAPRA"
     gene            7388..8242
                     /locus_tag="VNU01_12585"
     CDS             7388..8242
                     /locus_tag="VNU01_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH33499.1"
                     /translation="MSATRAPGDPRRLHPDEVPALTRRLETGFATAGPAVASLLNQHL
                     LDALDQGEHERFVLWPGDDPVAVLYAAANSSAIPAGHPSAARALAGPLERSGWRVLIG
                     DAPLTQALVDTVPAPLFRRGPRVREQRFMVLRNPRRRVVAGLRPAGLADLEAVTEFAC
                     RLHEEDLMGPPISRSGRGAVRVRMMEATSRSAVWVVERGGKPVAKVDLSLCSRRRGAQ
                     VAGVYVLPEWRGHGIAAAAVGEIGAHLLEQGFPGVTLHVRSDNAAAIRAYERAGFTDE
                     AALVLALR"
     gene            8453..>10079
                     /locus_tag="VNU01_12590"
     CDS             8453..>10079
                     /locus_tag="VNU01_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH33500.1"
                     /translation="MALALVAGMLGAVPGSAAVSGEPAAAPAHDHASHDHGAPDARPA
                     VHDLLHVDPVVTIRELAPGEPLPSAPGIAGPAMGDGAQASEDGYATAADEDQSAMFLP
                     DFGGGSGGTTTAPSTRRPSSREPQGKVDRVSSPAPMVRPIMVSATVDYAGQVAPLVFP
                     EGALPYKFWVERALANRHGLGQVVGAVGQWDGIPGSRWATQHVGVVEERVGAAAADGR
                     SIIFYKSDCPAGVGGYAYWQTATGAADARYGDAAIYITEVDVGICTAVTNDEALRAVL
                     AHEIGHAIGMEHLCDPGQPCWKDGMGAGPHGCRVMYAASSSCRRVIGEPERTAAVHNY
                     PTIRRLSGPSRVETSARASFAGFSQRAAGAVVLARSDRTAHGPLAAAALSGALRGGFL
                     LGSPASSGCLTGAAAEELARAAADPGRVVLVGDWPQTCDTALAAWNLAVERVGAAADP
                     VAMGVDVAARVAASGRMGNSVFIVSAKADATGHVPDGVAAGAAAGANAAPVLYTSPEQ
                     LGAPVASWIRSQPGVRRAYVMGGPGAVSERVVQD"
BASE COUNT         1126 a         3717 c         3966 g         1270 t
ORIGIN      
        1 gttgctgggg ggtggcgacg acgtgctggg cgcggccctg gcggcacggc tgccgctggc
       61 gcgagcggcc gacgatgcct ggtcgctgcc ggcgcccctg ggcgacctca tccagcccgc
      121 gccactggag ccctcggagg cggtcgccgt ggccgagtgg tgcctggcgc gcgacctggc
      181 cgccgaggcg ttcgccgtgc tgtgggccgg cgggcacgac gaggccgcgc tcggactcct
      241 ggagcgctgc ggcggcgcgg aagcggaggc gctgctggcc ggctggaccg gacccctgga
      301 gctgccgtcc tggcgcctgc gcgacacacc cgtggccgcc gagcggctgg cgtcggcggc
      361 ggagcgcgcc ggccatccgc agcgggagcc gcgacggcgc ctggccgccg tccccgacgt
      421 ccacgggccc ctggcggggc agcgggagcg ggcgcagctt gcccgtgacc tgctgcagcg
      481 cggccatggg gcggaggcac gccgcgaggc ggccacgctg ctgcgcgaga cgaccgcgcc
      541 ggatgtccgc gccagggcgc tggagatcat cgccagggtg gcgcgccgcg ccggcagcgc
      601 cgaggagctg tccctggcgt cgacgctgtt gcgcgaggcg ctggcgctgt ggcggcggga
      661 ggacgaaccc ggacgcgtcg cggagacccg gctgcacctg gcctgggtgg accgcgaccg
      721 cggtcacagc gcgctggccc tggagcgcgt cgccaccgtg cggcgcggcg ccccgccagg
      781 cggcgcgctg tgggtgcagg ccaccctggc cgaggcggcg ctgctgtgcg acacgggacg
      841 gtgccaggcg gcgggcgccc tggtggacga gctcgagcgg gtggcgtggg cctgggcggg
      901 accgcgcgtg aagtcccggg ccgccgtggt ccgcgcctgg gcggccgtgc acgccgagca
      961 cgacgaggac gcggcccggc ggacggagga gatcaccgcg gacctcctgg gctggatgga
     1021 cggcgtctgc ggcctgcgca acctggccgc cgccgccgag atctgggccc ggctgggccg
     1081 gcacgaccgt gtggcgtggt gcctgcggct catcgacacg gtgcaggtgg cgccggactg
     1141 ggaggtcacg ctggcgcggg ccggcgcgga atgccgcatc ggcgacccgg tcgcggccat
     1201 ggcgatgctg cgcccgctgg agccgcccgg cgccgccccc ggccgggacc gctggcgcgt
     1261 gctgctgctg ggggcattcg ccgcgctgcg ggcaggcgcg caggacgcgc ctgcccgggc
     1321 ggtcgcggcg ctgcaggccg ccgtggccgc gggggacccc tatgcgccgt tcgtgcggga
     1381 gcgcgacctg gcccggcggg tcctggccgc ggcgggcccg gtcgccggag gtgccgggtg
     1441 agggtccgcg tcctgggccg cttcgcggtc acgcggccag gtgccgatgt caccccgacc
     1501 ggcatccagg cccagctgct caaggcactg gtgtgcggcg gccgccccat gcactgggaa
     1561 gaagtggtgg aagtggtgtg gccgggcacg ggtcccaagc gcggccgcat ccggctgcgc
     1621 aacgcgctga gccgcctgcg gggccggtgc ggaccggtgg cgatgcgcgc gggcgcgatg
     1681 ctcatgatcc cgcccgaggt cggtgtcgac gccgtggagt tcgaggcact ggcgcagcgg
     1741 gcgttggagg cggggccctc ggaggagggg ctgcgcctgg cacggcaggc cctggagcac
     1801 taccgcgggg acctgctgcc ggaggatcgc tacctcgact ggaccatgcc gttccgcgaa
     1861 cggctgcgca ggcgcttccg cgggctggtc gacctgctgg cgtccgacgc gcgagcacgc
     1921 ggggatttgg agggcgcgct gctgctgctg gagcgcgcct tggccgacga gcccgaggac
     1981 gaggggctgg ccctggacac cgtggagctc ctgctcgccc tggggcggtc cgggtcggcc
     2041 gtccggctgc tggaccgggt gcgggcggag ttggaccagc agggcgtccc cgtgtcggcc
     2101 cgctggggcg acctgcgccg gcaggcgcgt ggacccgagc agggcgccgg ctaggccttg
     2161 cggaagcggt cgacccaggc ccgcagcgtg tccgagccgg cggacgaggc ctgtgcccag
     2221 gactcgtact ccaggacggc gtccaggccg gcgtgctccg ccgccaggcg cacgctgcgc
     2281 ttgatgtgcc gtgccagcgc cggctcggcc accgcgaacc ggcgcgccag ttcgacggcg
     2341 gccgccaccg ggtccgcttc cggaccttcc gccaggcccc acgccacggc tcgctccgcg
     2401 tcgagcgtgt cacccaggag cagggtccgc agggctcgcg aggggccgag cgcctgcacc
     2461 aggaaccagg tgcagccacc gcccgggtgc aggccgatac gggcgaaggt cgcaccgaag
     2521 gcggcgtcgg gcccggccac acggacgtcg cacgccagcg ccaggttcag gccggcaccg
     2581 acggccgcgc cgttcaccgc ggcgatcgtg gggatggcca actcccgcag ggccaggaag
     2641 ccgcggtagt agcgctgcag gcgcgcgcgc gtctcctcga ccgggcggtc ggcgtcgccg
     2701 aacagggcgg gcaggtccgc gccggcgcag aacgcctcgc cctcgccggt gacgaccagc
     2761 gccttcgcgg ccgggtcggc cgccagtgcg gcggcggcgt cgacgagttc ggcgcccatc
     2821 gcctcgtcca tggcgttgcg ccggtcggga tcggtcagcg tgagcagcca gaccccgtct
     2881 cccagcgcgt cgcgtcgcgt cttggccacg tccctcgtcc tttcgccggg ggcagcctag
     2941 agcacgccgg gagcgctgtt cgcgactgtg cgtaatcgcg tggagaccgt atgcttttac
     3001 ggtttggcgg gagagagagc cgtcgcgcac gcggacggga cctttgaggg ggaatttcat
     3061 ggacagcagc acgcccacga cggcagggct ggagcgcatc gcgcccgtag agccgccgta
     3121 cgacgcggag gtcgacgccg agctcggccg ctggatgccg ccgggcagcc cgttcgagcc
     3181 gctgcaggtg ttccgggtgc tggcccgcaa cctgccgctg atgcagcgca tgctgccgct
     3241 cggctcgggc ctgctgaatc acccgtcggt cagccaccgg gaccgcgagc tgatcatcca
     3301 gcgcacgtgc gccctggccg gcgcggaggc cgagtgggcc acgcacgtct tcgccttcgg
     3361 caaggccgtc ggcatcacca gggagcagac cgcctcgctg gtcttcggcg gcccggccga
     3421 cgcctgctgg acggacgagc gcgaccgcct ggtcatcgag ctgtgtgacc agctgcacgc
     3481 gaccgcgacc gtgacgccgg acctgcgggg ccggttggcg cgggagttcg acgacggcca
     3541 ggtggtggag ctgctggtca tcgccggctg gtaccgcacg ttctcctacg tggtcaacgg
     3601 cgccggcgtc acgccggagg agttcgtccc ccgcttcacg gacttcccgc tgccgtagga
     3661 ccggtgcctg ccacggggac ggcacccgca cgggccggcg cggtgcccgg cgtgcctgac
     3721 tagactccgg tccgacgcgg acggaaaggt cgccggcacg tgagcacgtg ggaggaactg
     3781 cgcgcccagg cggcggcgtg cacgcgctgc cgcctggcgt cgggtcgtac ccaggtggtg
     3841 ttcggcgtgg gcgatccgga cgcctcgctc atgttcgtcg gtgaggctcc cggcttccac
     3901 gaggaccagc gcggcgagcc cttcgtcggg cgtgcgggca agctcctcga caccctgtgc
     3961 gccgacgtgg gcctgtcgcg cgagcgcggt gtctacatcg ccaacgtcct gaagtgccgc
     4021 ccgccggaca accgcgaccc gctgccggac gagatcgccg cgtgcaagcc gttcctgctg
     4081 gagcagcttg cgcacgtcga cccgggcgtc gtggtgacgc tgggggcgtt cgccacccgc
     4141 cccctgctgg ccgacgccga gccggggccg ccggatccgt cccgcgtgtc cgtgagcaag
     4201 gtggcggggt atcgcttcca cgtgcacggg cggacgctga tccccacgta tcacccggcc
     4261 gccgcgctgc gcggcaacgc ggcggcgctg gacgccctgc gccgcgacct gcgcctggcc
     4321 gccgacgtgc tggccggccg tgtgccgacg gcggcggagg ccctgggcct gagcgccccg
     4381 gcggagccgc cggcggccgc cccggccgcc gacgcgcccg gcggggaccc cgcgctccag
     4441 ttgaggctgc tctagatgag cgagtgcacg ctgcgcagcg cgtcgccgga ggagacccgc
     4501 gccatcggcg ccgcgctcgc cggtgcgctg cgcgccggcg atgtcgtgtc cctcaccggc
     4561 gagctcggtg ccggcaagac ctgcctggtc cagggtgccg cggccgccct cggcgtggcc
     4621 gagcgggtca ccagcccttc gttcctgctg cgcaaggact accaggggcg cgtgccggtg
     4681 ctgcacctgg acgtctaccg gctggagacg ctgcgcgacg tcatggacat cggctgggac
     4741 gaggcggtgg acggctcgcg cgtcagcttc gtcgaatggg gggatgccat gagccccctg
     4801 ctgccggagg agtatctcga ggtcgacctg cgcgccgtca acgacgacca ggctgccctg
     4861 ggcgaggagc ctcgccggct gctgttgcgc gcccgcggcg aggactgggc gcggcggctg
     4921 gccgagctcg tgccggcgct gaggcgctgg cgggacccgg gctcggcatg ctgatcctgg
     4981 gcatcgacac ctgcaccggc cggaccagcg tcgcgctggg ctccggcgac ggcgtgctgg
     5041 cgagcgcctc gctggcgcgc cccacggcgc acggagggtt cctggctccc gccatcgagt
     5101 tctgcctgcg gcaggccggc gccggggtgg gcgacctgca cggcgtcgcg gtgacgctgg
     5161 ggcccgggct gttcacgggg atgcgcgtgg gcatcgcgac ggcgcaggcg ctcgcgcacg
     5221 cgcgccggct gcccgtggtc ggcctggcgt cgctggacct gctcgcgttc cgggttcggc
     5281 acgcgcgccg ccccgtgtgc gcggtcctgg acgccaagcg caaggagctg ttctgggcct
     5341 tctaccggcc ggtccccggc ggggtgcagc gcgaggggga gttccgcgtg ggaccagccg
     5401 agaagctggc cgccgaactg gaggcctccg gccaggacac gctgtgcgtc ggcgacggcg
     5461 ccgtggccca ccggtcgctg ctggagtcgg ccggcgcgga cgtggacccg tcgcccgcgc
     5521 tggcctcccc gcacgccgag agcctggtgg agctggccgt gccgcgcttc ctgcgcgagg
     5581 agacgcagcg cgccgaggag ctgcgaccgg tgtacctgcg ccaggccgac gcgcgcatcg
     5641 gctggcggga gcgcggcgtg ctgtccggcg gcatggccgg tccctcgggc ggcgggccgg
     5701 ccgccgggga ggcaggctga tggtcatggc agcctcccgc cgcctcgcgc tctccggcgt
     5761 ccgcctggag ccgatgcacc cccggcacct gcgccaggtg ctggcgatcg agcaggcggt
     5821 gtacccgcgc ccctggacgc cgggagtgtt cctgtcggag ctggaggcgg gggactcgcg
     5881 ccgctactac gtggcgctcg cgggccgcgg tggacggctc gcgcggctgc gcggtccccg
     5941 cgtggtcggc tacggcggga tcatggtgca ggtcggcgag gcgcacgtga ccaccgtggc
     6001 ggtggacccg cgcgagcacc gccgcaaggt ggccagccgc ctgctggtgc gcctgctggc
     6061 ggacgcgcgc gccatgggcg ccgaggcggc gaccctggag gtgcgcacca ccaacctggg
     6121 cgcgcagcgg ctgtaccagg cgttcgggtt cgcgcccgtc ggggtgcggc ccggctacta
     6181 cgccgagacc ggcgaggacg cgctcatcat gtgggcgcac gagctgcagg gcgagcggat
     6241 gcggcagcgg ctggaggagc aggccgccag gctggaccag cctggcggcg ccagcggcca
     6301 gcccgacctg gaggtgccgt gggtgcgcgg ccgcaccgga ctggagggct gacgtgctgg
     6361 tgctgggcat cgagacgtcc tgcgacgaga ccgccgtggc gctggtcgag gacggactgc
     6421 gcgtgcgctc caacgtcatc ggcacgcagg tggaccagca ccgcccctac ggcggcgtgg
     6481 tgccggagat cgccgcccgc gcgcacctgg agctcatgct gccggtgctg gaccgcgcgc
     6541 tgatcgaggc cggctgcacc tacgacgacg tggagggcat cgccgtcacc gtgggccccg
     6601 gcctggtcgg ggccctgctg gtgggcgtcg cggccgccaa gtcgctggcg ctggcgcgcg
     6661 acgtgccgct gatcggggtc aaccacctgg aggggcacat ctgcgcgacc cagctcgaat
     6721 tcggccccct cgcgctgccg ctggtcgccc tcatcgtcag cggcggccac accagcgtca
     6781 tcctgctgga cgaggacggc cattcccgaa ccctcggcgc gaccatcgac gatgcggccg
     6841 gggaggcgtt cgacaaggtg gcgcgcttcc tggggctgcc gtacccgggc gggccggaga
     6901 tcgaccggct cgcccgcgac ggcgaccccg ccgccatcgc gttcccccgg gcgctcatgc
     6961 acgagcgcgg ctacgacttc tccatgtccg gcctgaagac cgccgtggtg cgcgagctgc
     7021 gccggcgcga ggcggccggc gagccgatcc acctgccgga cgtggccgcg tcgttccagg
     7081 aggcgctggt cgacgtccag gtgcgcaaga ccgtcgcggc cgcggtcgac cacggcgtgc
     7141 cgaccgtgac cgtggtcggc ggtgtggcgg cgaactcccg gctgcgccaa cgcatggagc
     7201 aggcgtgcgc caaggcgggc ctgcgcctgc tggtgccgtc gccggcgctg tgcaccgaca
     7261 acggcgccat gatcgcggcc gccggcttca accgcctggc cgccggcgag cgcacggcgc
     7321 tgtccgtgga cgccgacccg aacctgccgc tgcggccgat cccggtcgcg ccggccgccc
     7381 cgcgcgcgtg agcgccaccc gggccccggg tgacccgcgc cggctgcatc ccgacgaggt
     7441 ccccgcgctc acccgccgcc tggagaccgg cttcgccacg gcgggtcccg ccgtggcgag
     7501 cctgctgaac cagcacctgc tggacgcgct ggaccagggc gagcacgagc gcttcgtgct
     7561 gtggcccggt gacgacccgg tggcggtgct gtacgcggcg gccaactcca gcgcgatccc
     7621 ggcggggcat ccgtccgccg cccgtgcgct cgccgggccg ctggagcgca gcggctggcg
     7681 cgtgctcatc ggcgacgccc cgctgaccca ggcgctggtg gacaccgtgc ccgcaccgct
     7741 gttccgccgc gggccgcggg tgcgcgagca gcgcttcatg gtgctgcgca acccccggcg
     7801 gcgcgtcgtg gccggcctgc gcccggcagg gctggcggac ctggaggcgg tgacggagtt
     7861 cgcctgccgg ctgcacgagg aggacctcat ggggccgccc atctcgcgct ccggccgggg
     7921 cgcggtgcgc gtgcgcatga tggaggcgac gtcgcggtcg gcggtctggg tggtcgagcg
     7981 cggcgggaag ccggtggcca aggtggacct gtcgctgtgc agccgccgcc gtggcgcgca
     8041 ggtggccggc gtgtacgtgc ttccggaatg gcgcggccac ggcatcgccg ccgccgccgt
     8101 cggcgagatc ggcgcgcacc tgctcgagca gggcttcccc ggcgtcaccc tgcacgtccg
     8161 ctccgacaac gccgccgcca tccgcgccta cgagcgcgcc ggtttcaccg acgaggcggc
     8221 gctggtgctg gcgctgcgct gagccgtcgg acggcgccgt cacccgttcc ggggacacgg
     8281 gcactacgcc gtttggacca tgccgccgta gtcactctgg gcacgcggga acggccggat
     8341 cgggtgaggc cctcgccgtc gcttgctgcc gattcctact ccgacgtcac gaaacggaca
     8401 tggagtcgga tcgatgcagc cgcgcaggtc ttcccgcccg agctacccgc tcgtggcact
     8461 cgccctcgtc gccgggatgc tgggcgccgt gcccggcagc gcggcggtgt ccggcgagcc
     8521 ggcggccgcc ccggcgcacg accacgcgag ccacgaccac ggggcgccgg acgcgcgtcc
     8581 cgccgtccac gacctgctcc acgtcgaccc ggtcgtcacc atccgcgagc tggcgccggg
     8641 cgagccgctg ccgtccgcgc cgggcatcgc cggccccgcg atgggcgatg gcgcccaggc
     8701 gtcggaggac ggctacgcca ccgccgcgga cgaggaccag agcgccatgt tcctgccgga
     8761 cttcggcggg ggcagcggcg gcaccacgac cgcgccgagc acccggcgcc cgtccagccg
     8821 cgagccgcag ggcaaggtgg accgcgtcag ctccccggcg cccatggtgc gccccatcat
     8881 ggtgtcggcg acggtggact acgccggcca ggtcgcgccg ctggtgttcc cggagggcgc
     8941 gctgccgtac aagttctggg tcgagcgcgc gctggccaac cgccacgggc tcgggcaggt
     9001 cgtgggcgcc gtcggccagt gggacggcat cccggggtcg cgctgggcca cccagcacgt
     9061 cggcgtggtg gaggagcgcg tgggcgcggc cgccgccgac ggccgctcga tcatcttcta
     9121 caagtccgac tgcccggccg gcgtgggcgg ctacgcctac tggcagaccg ccaccggcgc
     9181 ggcggacgcc cgctacggcg acgccgcgat ctacatcacc gaggtggacg tcggcatctg
     9241 caccgcggtc accaacgacg aggcgctgcg cgccgtgctg gcgcacgaga tcggccacgc
     9301 catcggcatg gagcacctgt gcgaccccgg gcagccctgc tggaaggacg gcatgggcgc
     9361 cggcccgcac ggctgccgcg tgatgtacgc ggcgtcgtcc tcctgccgcc gcgtcatcgg
     9421 cgagcccgag cgcacggccg ccgtgcacaa ctacccgacc atccgccgcc tgtccggccc
     9481 cagccgcgtc gagacgtccg cccgcgcgtc cttcgccggg ttctcgcagc gcgcggccgg
     9541 cgcggtggtg ctcgcccgca gcgaccgcac cgcccacggc ccgctggccg cggccgcgct
     9601 gagcggcgcg ctgcgcggcg gcttcctgct cggctctccc gcgtcctccg ggtgcctgac
     9661 cggcgcggcc gccgaggagc tcgcccgtgc cgccgccgac cccggccgcg tggtgctggt
     9721 gggcgactgg ccgcagacgt gtgacacggc gctggccgcc tggaacctgg cggtggagcg
     9781 tgtcggcgcg gccgcggacc cggtggcgat gggcgtcgac gtcgccgccc gcgtggccgc
     9841 cagcggccgg atgggcaatt cggtgttcat cgtgtccgcc aaggccgacg ccacgggcca
     9901 cgtgccggac ggtgtcgccg ccggcgccgc cgcgggggcc aacgccgcac ccgtgctgta
     9961 cacctcgccg gagcagctcg gcgcgccggt ggcgtcgtgg atccgcagcc agcccggtgt
    10021 gcgccgcgcc tacgtgatgg gcggccccgg ggcggtgtcc gagcgcgtgg tgcaggacc
//
LOCUS       DATMKC010000433         3739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_82778, whole genome shotgun sequence.
ACCESSION   DATMKC010000433 DATMKC010000000
VERSION     DATMKC010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82778"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VNU01_12595"
     CDS             complement(<1..285)
                     /locus_tag="VNU01_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWH33501.1"
                     /translation="MTDGLDRLREAQRRTWAAGDYAEVGKNLVPASEAVVARAGIGPG
                     MAVLDVATGTGNAALLAAGAGADVTGVDFTPGLLEVARERARGAGLDAEFV"
     gene            complement(558..1286)
                     /locus_tag="VNU01_12600"
     CDS             complement(558..1286)
                     /locus_tag="VNU01_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HWH33502.1"
                     /translation="MRTDTLYSTDAYLRAFEGEVLEVDAEQGRVALSRTAFYPGGGGQ
                     PPDTGVLDWGAGRVPVARTSADGGLVWHWIAPEAAADLPAPGTTAEGVIDWDRRHLLM
                     RTHTALHILCGVIWADYRVAVTGGNMEPGEGRLDFPLDAMSAELGQWAQRRINEEIQR
                     AREILVDFVDRAEADRDEALIRTKANLIPASINPLRVIDIVGLDKQADGGTHVATTAE
                     VGRVEVTKTESKGKDNKRIRLAVR"
     gene            complement(1381..2250)
                     /locus_tag="VNU01_12605"
     CDS             complement(1381..2250)
                     /locus_tag="VNU01_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWH33503.1"
                     /translation="MAGVEGRVVVVTGSGGGLGRQHALLFAERGAKVVVNDLGGSRDG
                     TGAGSEMADNVVKEIQDAGGEAVANYDNVATTEGAQGIIKSALDAFGKVDIVVNNAGI
                     LRDVTFVKMEDSQWDSVLKVHLYGSYNVTKAAWTHMREQNYGRVIMTTSTSGLFGNFG
                     QSNYGAAKLGMVGMMNTLAMEGRKYNINVNAIAPIAATRMTEDILPEQMLQQFDPAFV
                     SPAVVHLASEECTDTGIIILAGGGQYARVAYFQSQGVQMGEVPGVDELAGKWAEITSL
                     EGAKPAGPFSAQS"
     gene            2464..>3739
                     /locus_tag="VNU01_12610"
     CDS             2464..>3739
                     /locus_tag="VNU01_12610"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH33504.1"
                     /translation="MPVPHRSWRRPALHVAVALAVLLAGLPFAAPAHAAAGDRWSLAG
                     QDPVTTAVALSRFAFPDGAPSALLARADAFPDALASGLLQDEGPLLLTAGAALDTAAR
                     EELARLGVERVTVLGGTAAITEAVAEELRQAGYRVSRLSGGTRVDTAVAVASSRPASR
                     TVLLARAFGGSGDASQGFADALSAGAWAAASGYPVLLSETASLSAATAAYLRAAAVRT
                     VIMVGGAAALSAQVEQQLLALGVGVRRVSGAERAATAVAVAEAQGWDSSADVSQVVVV
                     PGRGDSAWAAGFGAAALAAGGATAVVLAEGDALPTATRRFLRAGSADVVCAPLLARAR
                     CDEAAEELGTSSPELAAFAGTGAADQERGADGERSYTVTVDGGGPVTLALVDARASAA
                     GAPGWALDDDGDGAADLASGPVITGVGGRAVTG"
BASE COUNT          418 a         1407 c         1391 g          523 t
ORIGIN      
        1 cacgaactcc gcgtcgaggc cggcaccccg ggcgcgctcg cgcgcgacct cgagcagccc
       61 gggcgtgaag tccacgccgg tgacgtccgc tcccgccccg gcggccagca gcgccgcgtt
      121 gcccgtgccg gtcgccacgt ccagcaccgc catgccggga ccgatcccgg cgcgcgcgac
      181 caccgcctcc gaggccggca ccaggttctt gccgacctcg gcgtagtcgc ccgccgccca
      241 ggtccgccgc tgagcctcgc gcagccggtc caatccgtcg gtcatgccgg cgcgccgccc
      301 acgtccggga ccgtggcctc cggggtcgcg cggcgggccc ggcccagcac cgcccccgcg
      361 cgcggcgtga ccgcgaccag acggcgcatc agcaccaccg ccaggccgcg accgtcacgc
      421 ccaagccgat gaccgccccg acgcggtcgc ctgtgtccac cgcgttgccc gccatgacgc
      481 ctcctgtcgc gcctgcacga acgttcgatg cggacgctac gtcatcggcc gcaccgcggt
      541 gagcttcacc ggatccttca gcgcacggcg aggcggatgc gcttgttgtc cttgcccttg
      601 gactcggtct tggtgacctc cactcggccg acctcggcgg tagtggccac atgggtgccg
      661 ccgtcggcct gcttgtccag gccgacgatg tcgatgacgc gcagcgggtt gatcgacgcc
      721 gggatcaggt tggccttggt gcggatcagc gcctcgtcgc ggtccgcctc ggcgcggtcg
      781 acgaagtcga ccaggatctc gcgcgcccgc tggatctcct cgttgatccg ccgctgcgcc
      841 cactgcccga gctcggccga catggcgtcc agggggaagt ccaggcgtcc ctcgcccggc
      901 tccatgttcc cgcccgtcac ggcgacgcgg tagtccgccc agatgacgcc gcagaggatg
      961 tgcagggcgg tgtgggtgcg catcagcagg tgccggcggt cccagtcgat gacgccctcg
     1021 gcggtcgtgc ccggtgccgg caggtcagcc gcggcctccg gggcgatcca gtgccacacg
     1081 agcccgccgt cggcggacgt cctggcgacc ggcacgcgcc cggcgcccca gtccagcacg
     1141 ccggtgtccg gcggctggcc gcccccgccg gggtagaacg cggtgcggct cagcgcgacg
     1201 cggccctgct cggcgtccac ttccaggacc tcgccctcga acgcccgcag gtacgcgtcc
     1261 gtcgagtaca gcgtgtcggt tcgcacgtgg ccctcctcaa cgccgaagcg ggtgcagtcc
     1321 gggcactatg ccgcccgatc tgcacccgct tccagggaaa agctgtggat cagccgcgcg
     1381 ctagctctgc gccgagaacg gcccggccgg cttggcgccc tccaggctgg tgatctccgc
     1441 ccacttgccc gccagctcgt cgacgccggg cacctcgccc atctggacgc cctgcgactg
     1501 gaagtaggcg acgcgcgcgt actgcccgcc gccggccagg atgatgatgc cggtgtcggt
     1561 gcactcctcg gaggccaggt gcaccaccgc cggggagacg aaagccgggt cgaactgctg
     1621 gagcatctgc tccggcagga tgtcctccgt catgcgcgtc gcggcgatcg gcgcgatggc
     1681 gttgacgttg atgttgtact tgcgcccctc catggccagc gtgttcatca tgccgaccat
     1741 gccgagcttg gccgccccgt agttggactg gccgaagttg ccgaacaggc ccgaggtgga
     1801 cgtggtcatg atgacccggc cgtagttctg ctcgcgcatg tgcgtccacg ccgccttggt
     1861 gacgttgtac gagccgtaca ggtgcacctt caggacggag tcccactggg agtcctccat
     1921 cttcacgaag gtgacgtcgc gcaggatgcc cgcgttgttc accacgatgt cgaccttgcc
     1981 gaaggcgtcg agcgcggact tgatgatgcc ctgcgcgccc tcggtggtgg cgacgttgtc
     2041 gtagttggcc accgcctccc cgccggcgtc ctgaatctcc ttcaccacgt tgtccgccat
     2101 ctcgctgccc gcgcccgtgc cgtcccgcga gccgccgagg tcgttgacga ccaccttggc
     2161 gccgcgctcg gcgaacagca gcgcgtgctg acggcccagg ccgccgcccg aacccgtgac
     2221 gacgacgacg cgcccttcga ctccagccat gtcggttccg ctccttgtgc tctgtagtgg
     2281 aacgctcggc gcgggagcct accggaggtc ggccatggct gaccaagcgg gccggggccg
     2341 gcggggcacg gtcgcgagcg cttcaccatc ccggcggcct gccgaccggc acactggacc
     2401 gccgcccggt ggtcttgccg cgctggccgc gcacgcgtgg cccgcgccac agggagcatg
     2461 ccgatgcccg tgccgcaccg ttcatggagg cgcccggccc tgcacgtggc cgtggcgctg
     2521 gcggtgctgc tggccgggct gccgttcgcc gccccggcgc acgccgcggc gggggaccgc
     2581 tggagcctgg cgggtcagga ccccgtcacc accgcggtgg cgctgagccg gttcgcgttc
     2641 cccgacgggg cgccgtccgc cctgctggcg cgcgccgacg cgttccccga cgccctggcc
     2701 tccgggctgc tgcaggacga ggggccgctc ctgctcaccg cgggggcggc gctggacacg
     2761 gcggcccgcg aggagctggc gcggctgggc gtggagcgcg tcaccgtgct gggcggcacg
     2821 gcggcgatca cggaggccgt ggcggaggag ctgcgccagg ccggctaccg ggtgtcgcgg
     2881 ctgtccggcg ggacgcgggt ggacaccgcc gtcgccgtgg cctcgtcccg ccccgccagc
     2941 cgcacggtgc tcctggcccg tgccttcggc ggcagcggcg acgccagcca gggtttcgcc
     3001 gacgccctgt cggccggggc ctgggcggcc gcgagcggct acccggtcct gctgtcggag
     3061 acggcgtcgc tgtcggccgc cacggcggcc tacctgcgtg ccgcggccgt gcgcaccgtg
     3121 atcatggtgg gcggcgcggc cgccttgagc gcccaggtgg agcagcagct gctggccctg
     3181 ggcgtgggcg tgcgccgcgt gtccggggcc gagcgcgcgg ccacggcggt ggcggtggcg
     3241 gaggctcagg gctgggactc ctcggcggac gtgagccagg tggtcgtggt cccggggcgt
     3301 ggcgacagcg cgtgggccgc cggcttcggc gccgcggcgc tggccgccgg cggcgccacc
     3361 gcggtcgtgc tggccgaagg cgacgcgctg cccacggcca cgcggcggtt cctcagggcc
     3421 gggtccgcgg acgtggtctg cgcgccgctg ctcgcgcggg cgcgctgcga cgaggccgcc
     3481 gaggagctgg gcacgtcgtc gccggagctc gcggccttcg cgggcacggg cgcggcggac
     3541 caggagcgcg gcgcggacgg cgagcgctcc tacacggtca cggtggacgg cggcggcccg
     3601 gtcacgctgg cgctggtgga cgcgagggcg tcggcggcgg gagcgccggg ctgggccctg
     3661 gacgacgatg gcgacggcgc ggccgacctc gccagcgggc cggtgatcac cggcgtgggc
     3721 gggcgggcgg tgaccggcg
//
LOCUS       DATMKC010000434         2450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_10000, whole genome shotgun sequence.
ACCESSION   DATMKC010000434 DATMKC010000000
VERSION     DATMKC010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10000"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..359)
                     /locus_tag="VNU01_12615"
     CDS             complement(198..359)
                     /locus_tag="VNU01_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33505.1"
                     /translation="MSGVRQGAQGTSTSGVEKSPARRARDAARRRRQEQSWAAKAGPV
                     EVRRVDPAP"
     gene            complement(356..586)
                     /locus_tag="VNU01_12620"
     CDS             complement(356..586)
                     /locus_tag="VNU01_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33506.1"
                     /translation="MTDPYGPCPICKAEWVEEGPCGACGEFFTDCYGWVENPADPDDL
                     VQSHPPISWASPALTAPGGDTCPFCCPDGRAA"
     gene            complement(583..1809)
                     /locus_tag="VNU01_12625"
     CDS             complement(583..1809)
                     /locus_tag="VNU01_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33507.1"
                     /translation="MTTTAATRRDQSGGRGSARSRYLGKSVGRPAAPDPAEEAERLRE
                     ERGRRYRLLYAIWRVTSLPQTAFCARYLHGGADFATVQLRDGRAGWGGLRSCKSIHAC
                     PRCSPVIRQRRYEQIERLGLAHAKAGGGLGFLTVTLPHTKADPLADSLDTVLNGWRAV
                     QQSRAVRSLFTRLGVLGSIRATEITRGYRGWHPHTHLLMFTTRPLTAAELAEIEEAVY
                     KAWARYVARRGYGAPSREHGVVMRPVDMSRGTDGLSAYLAKVHDAFGKSTTIALEMAR
                     GDLKRGRKGSRTPFEIAESAAAGHGGDLKLWHEYEAATKGRSVIYVPPKLSALYGVED
                     VTDDQAVEEAESAPAVAALDAAEYALVVRYRACAAMLTAAELRGGDAVYERLRALIRR
                     DRVDAARAARKAAASP"
BASE COUNT          321 a          894 c          911 g          324 t
ORIGIN      
        1 ggccggggga gaagaacgct cccccgacca gcaagagcag gagacgggcc gcgctgcgga
       61 gctgcggacg ggctcgggga cggcgcggct acgccgcgcg cgggcgcggc agcgcccgca
      121 agtgcgcctg ccaagttggc agagagccgg ccccgagccc tcgggctacg tctgacaccc
      181 gggaccggcc ccctggacta cggagccggg tccacccggc gcacctcgac cgggccggcc
      241 ttggccgccc agctctgttc ctggcgtcga cgtcgtgccg cgtcgcgtgc gcgccgcgcc
      301 ggcgacttct cgacgcccga ggtcgacgtg ccctgcgcac cctgccggac gccgctcacg
      361 ccgcacgccc gtcaggacag cagaacgggc aggtgtcacc gcccggcgcc gtcagcgccg
      421 gcgatgccca ggagatgggc gggtgcgact gcacgaggtc gtcggggtcg gccgggttct
      481 cgacccagcc gtagcagtcg gtgaagaact cgccgcaagc accgcacggc ccctcttcca
      541 cccactcggc cttgcagatc gggcacggcc cgtacgggtc ggtcatggtg acgccgccgc
      601 cttcctcgcc gctctcgcgg catcgacacg atctcgccgg atcagcgccc ggaggcgctc
      661 gtacaccgcg tcaccgccgc gcagctcggc cgcggtcagc atggccgcgc acgcccggta
      721 ccggacgacg agcgcgtact cagccgcgtc gagtgcggcg accgccggcg cgctctcggc
      781 ctcctcgacg gcctggtcgt cggtcacgtc ctcgacgccg tagagcgcgg acaacttcgg
      841 cgggacgtag atgaccgacc ggcccttggt cgccgcctcg tactcgtgcc agagcttgag
      901 gtcgccgccg tggccggcgg ccgcgctctc ggcgatctcg aacggcgtgc gggagccctt
      961 gcggccgcgc ttgaggtcgc cgcgcgccat ctcgagcgcg atcgtcgtgc tcttgccgaa
     1021 cgcgtcgtgg accttcgcca ggtaggcgct cagcccgtcg gtcccgcggc tcatgtcgac
     1081 gggccgcatg accacgccgt gttcgcggga cggcgcgccg tagccacgtc gcgcgacgta
     1141 ccgcgcccac gccttgtaga ccgcctcttc gatctcggcc agctcggccg cggtgagcgg
     1201 gcgcgtggtg aacatgagca ggtgggtgtg cgggtgccag ccgcggtagc cgcgggtgat
     1261 ctcggtcgcc ctgatggagc ccaggacacc gaggcgggtg aagagggagc ggacggcgcg
     1321 tgactgctgc acagcacgcc agccgttcag gaccgtgtcg agggagtcgg cgagcgggtc
     1381 ggccttggtg tgcgggagcg tcaccgtcag gaagcccagc ccgccgccgg ccttggcgtg
     1441 cgcgagcccg agccgttcga tctgctcgta ccggcgctgc cggatcaccg gcgagcaccg
     1501 cgggcacgcg tggatgctct tgcaggaccg cagaccgccc cagccggccc gaccgtcgcg
     1561 cagctggacc gtggcgaagt cggcgccgcc gtggaggtac cgcgcgcaga acgccgtctg
     1621 cggcaacgac gtgacgcgcc agatcgcgta gagcagccgg taccgccggc cgcgctcctc
     1681 gcggagccgt tcggcctcct cggccgggtc cggtgccgct ggtctgccaa cagacttccc
     1741 taggtaccga gagcgggcgc taccgcgccc gcccgactgg tcacggcgcg tggccgcagt
     1801 cgtggtcatg cggcgtcctg gtgggtaccg tcagcgccag cacccgcccg gccttccggg
     1861 cccgcagtgg agaagccaac ggcgagaacc tgcggccggg gtgccgggcg gtctcctcgc
     1921 acggtcgatc cagcagagcc ctcagcaagc tccaagggtc cggtgggcga ggggccgccc
     1981 gccccgtgtt cggggtcggc caggagccgg cccagggtgc acggcgacgg cacgtgcatc
     2041 aggtcggtga gtacggcggc agagccgctc cagcggcgag gcatccaagg tccgttcggc
     2101 ggtccggtca cttccagcag tccctcagca gccgccatcg gaccaccaga tcggcccgag
     2161 gtgtgggacg tgcgggcaag atcagcgcga cacgccgaca acttttttcg gtcgcggcgt
     2221 gtcgcatggc gtgtcgggag ttggtgcacg cggcggggcc cccggcgccg ctggcgcttg
     2281 cgcgccggcc agccccttgc gtgcggctcg gggacgcgtg ggcaggcggg tggagaagcc
     2341 gtcaagggcg ctgcgcggtc ctgcggacgc ggctacgccg ccccttgact gccggcagcg
     2401 tcggcggctt gcagaagggc acggggacgg ccctagaagc aggctcctgc
//
LOCUS       DATMKC010000435         4770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_267828, whole genome shotgun sequence.
ACCESSION   DATMKC010000435 DATMKC010000000
VERSION     DATMKC010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267828"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VNU01_12630"
     CDS             <1..696
                     /locus_tag="VNU01_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33508.1"
                     /translation="DTPPTRNALDFLDAPNRLTDFLEGRFLKMFLRPGFSAGRAVGRA
                     AAFGTGLFMKAASRITGVAVLDDLAEFFQSFEGMYEGFKARAQQVYRLLQGPESAFVV
                     VASPEPPALREARYFLQRLAQEGMPTAGLVVNRVSMPPAALEVLDPAAEAAAMERLRD
                     GDAPQRLAAALLRVHLDGRDTARREQRAITAALHGVDPRALVEVPLLPTDVHDAATLL
                     LLGDWLVGKAAQP"
     gene            800..3322
                     /locus_tag="VNU01_12635"
     CDS             800..3322
                     /locus_tag="VNU01_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWH33509.1"
                     /translation="MPTSLTRFLVGSVAAIAAAALLVAVSLGPTAGMAATVLQRTEGG
                     LFDFPPLPQDLGSPDERSVVYARDGSVLAILHVENRKSVPLSQVPLHVQQAVLATEDA
                     DFYEHQGVNWRAILRAALGNLRAGEVTSGASTITQQVVKNAVTGGEVTLRRKVREAAY
                     AVELEKRMDKRAILELYLNEAYFANGVYGIATAAEYYFGKDVGDLTVAEAALLAGMIR
                     APERNDPRDAPERARARRDIVLTQMAVEGFITRQEAERLRATPMELDIHPLEDIRNPF
                     FVDYVRHLLKQSDALGPDVQARDRAVLRGGLAIHTTLDPRLQDIAQEVIAEALDRGED
                     PLAALTAVDPRSGEILALGFGPKPYGKGPGQTEVNPAVPGLGSSGRQAGSAFKAFQLV
                     AALEEGISPGFSFQAGSRYTYKDPACDGHTVGNYADASQGVLDMASATAVSSNTYFAR
                     LVDLTGPDKLVEVAKRMGITSDIPAVCSLVLGSGEVFPLDMASAFGVLANEGVRCPPY
                     AISRIVDRTGRVIMRAQPECERVVDKGIAARATSLLRGPIERGTASRNGRIGRPAAGK
                     TGTSQDYWNAWFTGFVPQLSTAVWVGHERNVPMMGHPSCPRGMTGGCVPTQIWSRFMR
                     RALDVLDLPVASFPAPPPIPTAAVPSVVGMTEEEATEVLEDAGFNVVPETVTDHRKAG
                     TVVRQEPGGGGRAPKGGLISISVSDGEGTMPLVPSLLGMTEEEARAALGQLDGEVEVA
                     VVREPAEEDRIGKVVAQSPEAGADATDLATITLTIGRARTADDPTPTPTGTPSETPSA
                     SATPTPTESTGPSPSPTVTEEPEPSPRPTRTRRPRPTESEQQ"
     gene            3336..4226
                     /locus_tag="VNU01_12640"
     CDS             3336..4226
                     /locus_tag="VNU01_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWH33510.1"
                     /translation="MTVWAEALTGLAAAGGLALGWASLVERRWFACRHHEVPALRGPG
                     TLRILHVSDLHLLPWQRDKLAFVERLAAQRPDLVVATGDLLGHPGAIEPCVAALRSLR
                     GDTPGIFVLGSNDFFAPGPRNPLAYFRGPSRLKGGQRRLETDRLVAGLVEAGWQLVDN
                     QRVTIPTARGAVDVAGLGDPHIRRDRPDALAGPPAGSAALRLGVVHAPYRRALDALTA
                     AGSELILAGHTHGGQVRIPGVGALVTNCDLPTRQARGLSRHGAAWLHVSAGLGTSMYA
                     PVRFCCRPEASLLTVSQRTG"
     gene            4275..4349
                     /locus_tag="VNU01_12645"
     tRNA            4275..4349
                     /locus_tag="VNU01_12645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4310..4312,aa:Pro,seq:cgg)
     gene            4385..4648
                     /locus_tag="VNU01_12650"
     CDS             4385..4648
                     /locus_tag="VNU01_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HWH33511.1"
                     /translation="MRTLYGARDHARGLARTFAWFTEECGELSRALFRGTAEEQRAEF
                     ADVLAWLASLADQAGVDLAEAAARYAEGCPRCGAAPCACAPAP"
BASE COUNT          607 a         1807 c         1759 g          597 t
ORIGIN      
        1 gacacgccgc cgacgcggaa cgcgctggac ttcctggacg cgcccaaccg gctcaccgac
       61 ttcctggaag gccgcttcct gaagatgttc ctgcgccccg ggttcagcgc cgggcgcgcg
      121 gtcggccgcg cggccgcgtt cggcacgggg ctgttcatga aggccgcgtc gcgcatcacc
      181 ggggtggccg tcctggacga cctggccgag ttcttccagt ccttcgaggg catgtacgaa
      241 ggcttcaagg cccgcgccca gcaggtctac cggctgctgc agggcccgga gtcggcgttc
      301 gtggtggtcg cctcgccgga gccgccggcc ctgcgcgagg cgcgctactt cctgcagcgc
      361 ctggcgcagg agggcatgcc cacggccggg ctcgtggtca accgcgtgtc catgccgccg
      421 gcggccctgg aggtgctgga ccccgccgcg gaggcggcgg ccatggagcg gctgcgcgac
      481 ggcgacgcgc cgcagcggct ggccgcggcg ctgctgcgcg tgcacctgga cggccgcgac
      541 acggcccgcc gggaacagcg ggccatcacc gcggcgctgc acggcgtgga cccgcgcgcc
      601 ctggtggagg tgccgctgct gcccacggac gtgcacgacg cggcgaccct gctgctgctg
      661 ggcgactggc tggtgggcaa ggccgcgcag ccctgatttg cgccaggcgc acggcccggc
      721 ggcgcccgcg cccctatcat gacggcgtcc ccccggcacg ccgcaccggc gtccctctca
      781 cccgggcggg acgtcctcca tgcccacatc gctcacgcgc ttcctcgtcg gctcggtggc
      841 ggccatcgcc gccgccgcac tgctcgtggc cgtgagcctg ggccccaccg ccgggatggc
      901 ggccacggtg ctgcagcgca cggagggcgg gctgttcgac ttcccgccgc tgccccagga
      961 cctgggcagc ccggacgagc gcagcgtggt gtacgcgcgc gacggcagcg tgctggcgat
     1021 cctgcacgtg gagaaccgca agtccgtgcc gctgtcgcag gtccccctgc acgtccagca
     1081 ggcggtcctg gccaccgagg acgccgactt ctacgagcac cagggcgtga actggcgcgc
     1141 gatcctgcgc gcggcgctcg gcaacctgcg cgcgggcgag gtcacctccg gcgcgtccac
     1201 gatcacccag caggtggtca agaacgccgt caccggcggc gaggtcacct tgcggcgcaa
     1261 ggtgcgggag gccgcctacg cggtcgagct cgagaagcgc atggacaagc gcgcgatcct
     1321 ggagctgtac ctgaacgagg cgtacttcgc caacggcgtg tacggcatcg ccaccgcggc
     1381 ggagtactac ttcggcaagg acgtcggcga cctgacggtg gccgaggcgg ccctgctggc
     1441 cggcatgatc cgcgcgccgg agcgcaacga cccccgcgac gcgccggagc gcgcgcgggc
     1501 ccgtcgcgac atcgtcctga cgcagatggc cgtggagggc ttcatcacgc ggcaggaggc
     1561 ggagcgcctg cgggccacgc cgatggagct ggacatccac ccgctggagg acatccgcaa
     1621 cccgttcttc gtcgactacg tgcggcacct gctcaagcag tccgacgccc tggggccgga
     1681 cgtgcaagcg cgtgaccgcg cggtcctgcg cggcggcctg gcgatccaca ccaccctgga
     1741 cccgcgcctg caggacatcg cccaggaggt catcgccgag gccctggacc ggggcgagga
     1801 cccgctcgcg gcgctcaccg ccgtcgaccc gcgcagcggc gagatcctcg cgctgggctt
     1861 cgggcccaag ccctacggca aggggccggg ccagaccgag gtgaacccgg cggtgccggg
     1921 gctcggctcc agcggccgcc aggccggctc ggcgttcaag gcgttccagc tcgtggcggc
     1981 cctggaggag ggcatctcac ccggcttctc cttccaggcc ggcagccgct acacctacaa
     2041 ggaccccgcg tgcgacggtc acacggtggg caactacgcc gacgcgtcgc agggcgtgct
     2101 ggacatggcg tcggccaccg cggtgtccag caacacctac ttcgcgcggc tggtggacct
     2161 caccggaccc gacaagctgg tggaagtggc caagcgcatg ggcatcacca gcgacatccc
     2221 cgcggtgtgc tcgctggtcc tgggctccgg cgaggtcttc ccgctggaca tggcgtccgc
     2281 gttcggcgtc ctggccaacg agggcgtgcg ctgcccgccg tacgcgatca gccgcatcgt
     2341 cgatcgcacc gggcgcgtga tcatgcgcgc ccagccggag tgcgagcgcg tggtcgacaa
     2401 gggcatcgcg gcgcgcgcca cgtccctgct gcgcggcccg atcgagcgcg gcaccgccag
     2461 ccgcaacggc cgcatcggcc gtcccgccgc cggcaagacc ggcacctcgc aggactactg
     2521 gaacgcctgg ttcaccggct tcgtgccgca gctgtccacc gcggtctggg tgggccacga
     2581 gcgcaacgtg ccgatgatgg gccacccgtc gtgcccgcgc ggcatgaccg gcgggtgcgt
     2641 gccgacgcag atctggtcgc gcttcatgcg gcgcgccctc gacgtgctgg acctgccggt
     2701 ggcgtcgttc cccgcgccgc cgcccatccc caccgccgcc gtgcccagcg tcgtcggcat
     2761 gacggaggaa gaggccacgg aggtcctgga ggacgccggc ttcaacgtcg tgccggagac
     2821 cgtcaccgac caccgcaagg ccggcacggt ggtgcggcag gagcccggcg gcggcggacg
     2881 ggcgcccaag ggcgggctga tctcgatctc cgtctccgac ggcgagggca cgatgccgct
     2941 cgtcccgtcg ctgctcggca tgaccgagga ggaggcccgc gcggcactgg ggcagctcga
     3001 cggcgaggtc gaggtcgccg tcgtgcggga gccggctgag gaagaccgca tcggcaaggt
     3061 ggtcgcgcag tcgccggagg ccggcgccga cgcgacggac ctggccacga tcaccctgac
     3121 gatcgggcgt gcccgcaccg ccgacgaccc gacgcccacg cccacgggga caccgtcgga
     3181 gacgccgagc gcgtccgcca cgccgacgcc cacggagagc acgggcccgt cgcccagccc
     3241 cacggtcacc gaggagcccg agcccagccc gcgcccgacg cgcacgcgcc ggccacggcc
     3301 caccgagtcc gagcagcaat gaccccgggc ccggcatgac ggtctgggcg gaggcgctca
     3361 cgggcctggc cgccgccggc ggcctggccc tgggctgggc cagcctggtg gagcgccgct
     3421 ggttcgcgtg ccggcaccac gaggtgcccg ccctgcgcgg cccggggacg ctgcggatcc
     3481 tgcacgtgtc ggacctgcac ctgctgccgt ggcagcgcga caagctcgcc ttcgtggagc
     3541 gcctcgccgc gcagcggccg gacctggtgg tggccaccgg cgacctgctg ggccaccccg
     3601 gcgcgatcga gccgtgcgtg gccgccctgc ggtcgctgcg cggcgacacg ccgggcatct
     3661 tcgtcctggg ctccaacgac ttcttcgccc ccgggccgcg caacccgctg gcctacttcc
     3721 gggggccgag ccggctgaag ggcggccagc ggcgcctgga gaccgaccgg ctggtggccg
     3781 gcctggtgga ggcgggctgg cagctggtgg acaaccagcg ggtgacgatc ccgacggcca
     3841 ggggcgccgt cgacgtcgcc ggcctgggcg acccgcacat ccgccgcgac cgtccggacg
     3901 cgctggccgg cccgcccgcc gggtcggcgg cgctgcggct gggcgtggtg cacgcgccct
     3961 accggcgcgc cctggacgcc ctgacggcgg caggcagcga gctgatcctg gccggtcaca
     4021 cgcacggcgg ccaggtgcgg atccccggcg tgggcgccct ggtgaccaac tgcgacctgc
     4081 ccacccgcca ggcgcgcggg ctgtcgcgcc acggtgcggc gtggctgcac gtgtccgccg
     4141 ggctgggcac gtccatgtac gcgccggtgc gcttctgctg caggcccgag gcgagcctgc
     4201 tcaccgtgtc gcagcgcacg ggctgagggc gctttgtcgg ccctgcgccg taccgctacg
     4261 cttggcccgc tacacgggat gtagcgcagc ttggttagcg cgccacgttc gggacgtgga
     4321 ggccccgggt tcaaatcccg gcatcccgac tcatcacatg gatctcgccg acttccaacg
     4381 acagatgcgt acgctctacg gagcgcgtga ccatgcgcga ggtctggcgc gcacgttcgc
     4441 gtggttcacg gaggagtgcg gcgagctgtc ccgcgccctg ttccgcggga cggcggagga
     4501 gcagcgcgcc gagttcgccg acgtgctcgc atggctggct tccctggcgg accaggccgg
     4561 cgtcgacctg gccgaggccg cggcccgcta cgccgagggc tgcccgcgct gcggcgccgc
     4621 cccgtgcgcc tgcgcccccg ccccatgagc gccaccacgg acggtcggct gcccgacgag
     4681 gcgttcctgg tgcgcccgct gcggccggac gaggccgacg acgccctgcg ccgctcggtc
     4741 gagtccttcg gcgggcccgc gccgcacggg
//
LOCUS       DATMKC010000436         6736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_179212, whole genome shotgun sequence.
ACCESSION   DATMKC010000436 DATMKC010000000
VERSION     DATMKC010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179212"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VNU01_12655"
     CDS             <1..1023
                     /locus_tag="VNU01_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWH33512.1"
                     /translation="ACGDTTEDEPTGEGEETTDTGGEASDVRVGMIYDIGGRGDLSFN
                     DSAAAGLDRAKEEFGVEAKELEPDEGGENREELLRLLSEEQYDLVFGVGFLFADAIKA
                     ASADFPEVNYGLIDGGFEGLEEGGNVASLTFAEHEGSFLVGAAAALKSQTGKIGFIGG
                     VEIDLIKKFEAGYIAGAKHVNPDIEVDVKYISQPPDFSGFADPAKGKEIALGQYNGGA
                     DVIYHAAGGSGAGLFEAAKQVSEESGTKVWAIGVDSDQYQTAPEEVKEYILTSMLKRV
                     DVAVYETIKAFVEGDFSGGSQAFGLAEDGVGYSTSGGHVDDIADQLEELKQQIIDGEI
                     EVPTAP"
     gene            1067..2611
                     /locus_tag="VNU01_12660"
     CDS             1067..2611
                     /locus_tag="VNU01_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH33513.1"
                     /translation="MTDQEPGATPAIELRGIGKRFPGVVANDDVNLAILPGEVHAIVG
                     ENGAGKSTLMKILYGMQRPDEGEIRVAGEPVAFRSPKDAIAAGIGMVHQHFMLAGNLT
                     VLENVVLGAEPRRFGALDWSEARWRLDAMARAHDTPLDPDRTVDSLSVGEKQRVEILK
                     VLYRGARVLILDEPTGVLVPQEVDALFATLRELKAQGVTIAFISHKLHEVLAIADRVT
                     VMRAGRTVTTVDAGSVTTGELAELMVGSELPSPSTRTTHVTDVPELEVAGLGARGEGD
                     RWLVEGIDLSVRRGEIAGVAGVEGNGQAELVELLLGLRHADAGTIRLGGEDVTAWPTR
                     RRREAGMGYVPEDRHARGLLLSAPLWENVMLGRQGAEPFAHRGILRPKAARERTREVI
                     ERFDVRTPGEHVAAHALSGGNQQKLIMGREILGLPKLLIAAHPTRGVDVGAQAAIWEE
                     IRNARDAGLAVLLISADLDEIIGLSDRLHVMFRGRLVAELDPDEVTPEVLGSYMTGAR
                     GQVGAA"
     gene            2608..3753
                     /locus_tag="VNU01_12665"
     CDS             2608..3753
                     /locus_tag="VNU01_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33514.1"
                     /translation="MSRARSTAIAALRSAAPVLAVLFALAVSSLVLLLIGESPVGTFA
                     AMLRYASRLDSIVSILNRAIPLFLAGLAVAAGFRMGLFNIGVEGQYRISALVAAYLGA
                     IVTLPTGVHLVFVALVGAVVGGLWAGVAALLKVYRGVHEVISTIMLNFIATGLAAYLL
                     AEHFREPAVPGDLQIKTAALPASAMMPSLNWLLEGLGLELRRGTHLQGFLLVAVLIGA
                     AFWFLVARTRFGYDLRASGTNPLAAQASGVDQGRMILKVMFLSGALAGLVGVSQLVGF
                     FGRYTIDFPVGLGFAGIAVALLGRNTAVGTALAALLFGFLDRSAQILDLEDVPKEITT
                     IVQGVIILAVVIAGEIARRAARTQEAVAVSQEVAGEPEAAAAGGGRA"
     gene            3750..5060
                     /locus_tag="VNU01_12670"
     CDS             3750..5060
                     /locus_tag="VNU01_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33515.1"
                     /translation="MTAVEHRPEPDRGPAAPRRAARRTTWARWQRPLLFGFGALLLLS
                     VVARVTDSPELVSGNTYGAAFRLAIPILLAGLGGLFSERVGVVNIGLEGMMILGTWAG
                     AWAAWQHGPWWGVAAAIVAGALGGLLHAVATVTFGVDHVVSGVAINILAAGAMRFLSV
                     VTYTAGSGGGATQSPRISGGTGSASVPFLAGGDLFGWRTPNLLAWLEARDVFGLSDVA
                     ALVRGPAETMTWLLALAVVMVPVTWYVLWRTPLGLRMRSVGEHPLAAESLGVRVYGVK
                     YLGVTISGALAGLGGVVLVLEFAGIYREGQTAGRGFIALAALIFGNWRPGGVAGAAGL
                     FGYSDALQLRSESAVHGLLLFVALVLLGAAAWALWRRRLPRAAVMGALGVLFFWWYAT
                     TDTVPGQFVFFTPHLVTLLVLAFAAQRLRPPAAVGRPYRRGQLE"
     gene            5097..6536
                     /gene="pyk"
                     /locus_tag="VNU01_12675"
     CDS             5097..6536
                     /gene="pyk"
                     /locus_tag="VNU01_12675"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWH33516.1"
                     /translation="MRRAKIVATLGPALDEPERLRAAVEAGIDVVRLNFSHGEHADHA
                     RRLEMVRAVAQRTGRSVGALADMQGPKIRLGSLPETGVPVATGSEVFLIPGTERLDAY
                     DSGGHAALPVVYEHLAADVSPGSLVLIDDGSIRLVVSRVEPGGVWARVVAGGVARSRK
                     GVNLPGVAVSAPSMTDKDYVDIAAAVEMGADWIAMSFVRRPSDVLEARARVHALGGEA
                     PIIAKLERPEAITALEAILEASDAVMVARGDLGVEIGPERVPAIQKEIIAQANAQARP
                     VITATEMLESMIGSPRPTRAEASDVANAVFDGTDALMLSAETATGAYPVEAVRTMARI
                     IEVAEASPQLSHPAPAPRFELSIGRVVARAAVQVARDVRAAAIVVYSITGSSIQLVST
                     YRPDVPLLGLSPSDRVCRRTALMWGSEAAHVPMKEHTADLAMAAERLLVDSQRAGRGD
                     LVVLVSGRPGGQGGTNRIMVHRVGDPISG"
     gene            complement(6542..>6736)
                     /locus_tag="VNU01_12680"
     CDS             complement(6542..>6736)
                     /locus_tag="VNU01_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33517.1"
                     /translation="VLTDAGHELLDSHFPHHYRLQSNLVADLSPDEQEQLVTLLRRIR
                     GAVPSWLREPGSNGEAPSSA"
BASE COUNT          866 a         2354 c         2559 g          957 t
ORIGIN      
        1 gcctgcggcg acaccaccga ggacgagccc acgggcgagg gcgaagagac cacggatacc
       61 ggcggcgaag cctccgatgt ccgggtcggc atgatctacg acatcggcgg gcgcggcgac
      121 ctgtcgttca acgactcggc cgcggcgggc ttggaccgcg cgaaggagga gttcggcgtc
      181 gaggccaagg aactggagcc ggacgagggc ggtgagaacc gcgaggagct gctgcgcctg
      241 ctgtcggagg agcagtacga cctggtgttc ggggtcggct tcctgttcgc cgacgccatc
      301 aaggcggcat cggcggactt cccggaggtc aactacgggc tgatcgacgg cggcttcgag
      361 ggcctggagg agggcggcaa cgtcgcctcc ctgacgttcg ccgagcacga gggcagcttc
      421 ctggtcggcg cggccgcggc gctgaagtcg cagacgggca agatcggctt catcggcggt
      481 gtggagatcg acctgatcaa gaagttcgag gccggctaca tcgcgggcgc caagcacgtg
      541 aaccccgaca tcgaggtcga cgtcaagtac atctcccagc cgccggactt ctccggcttc
      601 gccgaccccg ccaagggcaa ggagatcgcg ctgggccagt acaacggcgg cgccgacgtg
      661 atctaccacg cggccggcgg ctccggcgcg gggctgttcg aggcggccaa gcaggtctcg
      721 gaggagagcg gcaccaaggt ctgggcgatc ggggtcgact ccgaccagta ccagaccgcc
      781 ccggaggagg tgaaggagta catcctcacc tcgatgctca agcgcgtcga cgtcgcggtc
      841 tacgagacca tcaaggcgtt cgtcgagggc gacttctccg gtggcagcca ggcgttcggc
      901 ctcgccgagg acggcgtcgg ctactccacc tcgggcggcc acgtcgacga catcgccgac
      961 cagctcgagg agttgaagca gcagatcatc gacggcgaga tcgaggtgcc gacggcgccc
     1021 tagcgccatc gacggacgga aggccggcgc cgttgccatg acaggcgtga cggatcagga
     1081 gccaggcgcg acgcccgcca tcgaactccg gggaatcggc aagcggttcc ccggagtcgt
     1141 ggcgaacgac gacgtcaacc tggcgatcct gcccggagag gtgcacgcca tcgtcggcga
     1201 gaacggcgcc ggcaagtcca ccctgatgaa gatcctctac gggatgcagc gtcccgacga
     1261 gggcgagatc cgggtggccg gggagccggt ggccttccgc tccccgaagg acgccatcgc
     1321 cgcgggcatc ggcatggtgc accagcactt catgctggcg ggcaacctca ccgtgctcga
     1381 gaacgtggtc ctgggcgcgg agccgcgccg cttcggcgcg ctggactggt cggaggcgcg
     1441 ctggcgcctg gacgccatgg cgcgcgccca cgacaccccg ctcgaccccg accgcacggt
     1501 cgactccctg tcggtgggcg agaagcagcg ggtggagatc ctcaaggtgc tctaccgggg
     1561 cgcccgcgtg ctcatcctgg acgagcccac cggcgtgctg gtgccccagg aggtcgacgc
     1621 gctgttcgcg accctgcgcg agctcaaggc gcagggcgtc acgatcgcct tcatctccca
     1681 caagctccac gaggtgctgg ccatcgccga ccgtgtcacc gtcatgcgcg ccgggcgcac
     1741 cgtcaccacg gtggacgcgg gcagtgtcac caccggcgag ctggcggagc tcatggtcgg
     1801 cagcgagctg ccgagcccgt ccacgcgcac cacgcacgtc accgacgtcc cggagctgga
     1861 ggtggccggc ctgggcgcgc ggggcgaggg ggaccgctgg ctggtcgagg gcatcgacct
     1921 gtccgtgcgc cgaggcgaga tcgccggcgt cgcgggcgtg gagggcaacg gccaggcgga
     1981 gctggtggaa ctgctcctgg gcctgcgcca cgccgacgcg gggacgatcc gcctgggcgg
     2041 cgaggacgtc accgcctggc cgacgcgccg ccggcgcgag gccgggatgg gctacgtgcc
     2101 ggaggaccgc cacgcgcgcg ggctgctgtt gtccgcgccg ctgtgggaga acgtgatgct
     2161 gggccgccag ggcgccgagc cgttcgcgca ccgcggcatc ctgcggccca aggcggcgcg
     2221 ggagcggacc cgggaggtca tcgagcgctt cgacgtgcgc acgccgggcg agcacgtcgc
     2281 cgcccacgcg ctgagcggcg gcaaccagca gaagctcatc atgggccggg agatcctggg
     2341 cctgccgaag ctgctgatcg ccgcgcatcc cacccgtggt gtcgacgtcg gcgcgcaggc
     2401 cgcgatctgg gaggagatcc gcaacgcgcg cgacgcgggc ctggccgtgc tgctgatctc
     2461 cgccgacctc gacgagatca tcggcctgtc ggatcgcctg cacgtgatgt tccgcggccg
     2521 gctggtcgcc gagctggacc cggacgaggt cacgcccgag gtgctcggct cgtacatgac
     2581 gggcgcgcgc gggcaggtgg gcgccgcgtg agccgcgcca ggagcaccgc catcgcggcc
     2641 ctgcgctccg ccgcgccggt gctcgcggtg ctgttcgccc tggccgtgtc gagcctggtg
     2701 ctgctgctga tcggtgagag cccggtcggc acgttcgcgg cgatgctgcg ctacgcctcc
     2761 cggctggact cgatcgtgtc catcctcaac cgcgccatcc ccctgttcct cgcgggcctg
     2821 gcggtggccg ccggcttccg catgggcctg ttcaacatcg gtgtggaggg ccagtaccgc
     2881 atctccgcgc tggtggcggc ctacctgggt gcgatcgtga cgctgcccac cggcgtgcac
     2941 ctggtcttcg tggcgctggt cggcgccgtg gtgggcggcc tgtgggccgg cgtcgccgcg
     3001 ctgctgaagg tctaccgcgg cgtgcacgag gtcatctcca cgatcatgct caacttcatc
     3061 gcgacgggcc tggccgccta cctgctggcc gagcacttcc gtgagcccgc cgtgccgggc
     3121 gacctgcaga tcaagaccgc ggcgctgccg gcctcggcca tgatgccgtc gctcaactgg
     3181 cttctggagg ggctgggcct ggaactgcgg cggggcacgc acctgcaggg cttcctgctg
     3241 gtcgcggtgc tgatcggggc ggcgttctgg ttcctggtcg cgcgcacccg cttcggctac
     3301 gacctgcgcg cgtcggggac caacccgctg gccgcgcagg ccagcggcgt ggaccagggc
     3361 cgcatgatcc tgaaggtgat gttcctgtcg ggcgcgctcg ccggcctggt cggcgtgtcg
     3421 cagctggtgg gcttcttcgg ccgctacacc atcgacttcc ccgtcgggct gggcttcgcc
     3481 ggcatcgccg tggcgctgct cgggcgcaac accgccgtgg gcaccgccct ggccgccctg
     3541 ctgttcggct tcctggaccg ctccgcccag atcctggacc tcgaggacgt gcccaaggag
     3601 atcaccacca tcgtccaggg cgtcatcatc ctggccgtgg tgatcgccgg ggagatcgcc
     3661 cgccgcgcgg cgcgcacgca ggaggccgtg gcggtgtccc aggaggtggc cggcgagccc
     3721 gaggcggcgg cggccggggg aggccgcgcg tgaccgccgt cgagcaccgg ccggagcccg
     3781 accgcggccc cgccgcgccg cgccgcgcgg cgcgccgcac cacgtgggcg cgctggcagc
     3841 gcccgctgct gttcggcttc ggcgccctgc tgctgctgtc ggtggtggcg cgcgtcaccg
     3901 actcgccgga gctggtgtcc ggcaacacct acggcgcggc gttccgcctg gccatcccga
     3961 tcctgctggc gggactgggc gggctgttct ccgagcgcgt cggcgtggtc aacatcggcc
     4021 tggaaggcat gatgatcctg ggcacctggg cgggcgcctg ggcggcctgg cagcacggcc
     4081 cctggtgggg cgtggccgcc gcgatcgttg ccggtgcact gggcgggctg ctgcacgccg
     4141 tggccacggt gaccttcggc gtcgaccacg tcgtcagcgg cgtggccatc aacatcctgg
     4201 ccgccggcgc catgcgcttc ctgtccgtgg tcacctacac cgccggcagc ggtggcggcg
     4261 ccacccagtc gccccgcatc agcggcggca cgggcagcgc cagcgtgccg ttcctggcgg
     4321 gcggggacct gttcggctgg cgcaccccga acctgctggc gtggctggag gcgcgcgacg
     4381 tgttcggact gtcggacgtc gcggcgctgg tgcgcggccc ggcggagacc atgacctggc
     4441 tgctcgcgct cgcggtcgtg atggtgccgg tcacctggta cgtcctgtgg cgcacgccgc
     4501 tggggctgcg catgcgctcc gtgggcgagc accccctggc ggcggagtcc ctgggcgtgc
     4561 gcgtgtacgg ggtgaagtac ctgggcgtca cgatctccgg cgcgctcgcg ggcctcggcg
     4621 gcgtggtgct ggtgctggag ttcgccggga tctaccgcga agggcagacc gcgggccgcg
     4681 gcttcatcgc cctggccgcg ctgatcttcg gcaactggcg ccccggcggg gtcgccggcg
     4741 ccgcgggcct gttcggctac tccgacgcgc tgcaactgcg ctcggagtcc gccgtgcacg
     4801 gcctgctgct gttcgtggcc ctggtcctgc tcggcgccgc cgcgtgggcg ctgtggcggc
     4861 ggcggctgcc gcgtgccgcg gtcatgggcg ccttgggcgt gctgttcttc tggtggtacg
     4921 ccaccacgga caccgtgccg ggccagttcg tgttcttcac tccgcatctg gtgacgctgc
     4981 tggtgctggc gttcgccgcg cagcgcctgc gtccccccgc cgctgtgggg cggccctacc
     5041 gccggggcca gctggaatag gacgaccacc cccgaccgag aacgacgagg agcaccgtgc
     5101 gacgcgccaa gatcgtggcc acgctggggc ccgccctgga cgagccggag cgtctgcgcg
     5161 cggccgtgga ggcgggcatc gacgtggtgc gcctcaactt ctcccacggc gagcacgccg
     5221 accacgccag gcggctggag atggtgcggg cggtggcgca gcgcacgggc cgcagcgtgg
     5281 gcgcgctggc cgacatgcag ggtcccaaga tccgcctggg cagcctgccc gagacgggtg
     5341 tgcccgtggc caccggctcc gaggtcttcc tcatcccggg cacggaacgc ctcgacgcct
     5401 acgacagcgg tggccacgcc gccctgccgg tggtgtacga gcacctcgcg gccgacgtca
     5461 gccccggctc gctggtgctc atcgacgacg gctccatccg cctggtggtc tcgcgggtgg
     5521 agccgggcgg cgtgtgggcg cgcgtggtcg cggggggagt ggcgcgcagc cgcaagggcg
     5581 tgaacctgcc cggcgtggcg gtgtccgccc cgagcatgac cgacaaggac tacgtcgaca
     5641 tcgccgccgc ggtggagatg ggcgcggact ggatcgcgat gtcgttcgtg cggcggccct
     5701 ccgacgtcct ggaggcgcgc gcccgcgtgc acgccctggg cggcgaggcg ccgatcatcg
     5761 ccaagctgga gcggcccgag gcgatcacgg cgctggaggc catcctggag gccagcgacg
     5821 ccgtcatggt ggcccgaggc gacctgggtg tggagatcgg gcccgagcgg gtgcccgcga
     5881 tccagaagga gatcatcgcc caggccaacg cccaggcccg cccggtgatc accgccacgg
     5941 agatgctgga gagcatgatc ggctcgcccc ggccgacccg ggcggaggcg agcgacgtcg
     6001 ccaacgccgt cttcgacggc accgacgccc tgatgctgtc ggcggagacg gccacgggcg
     6061 cctacccggt cgaggcggtg cgcaccatgg cgcgcatcat cgaggtggcc gaggcgtcac
     6121 cgcagctgtc gcacccggct ccggcgccgc gcttcgagct gtccatcggg cgcgtggtcg
     6181 cgcgcgcggc cgtgcaggtc gcccgtgacg tgcgcgcggc ggcgatcgtc gtgtactcga
     6241 tcacgggctc gtcgatccag ctggtcagca cgtaccggcc ggacgtgccg ctgctcgggc
     6301 tgtcgccgag cgaccgcgtg tgccggcgca ccgcgctcat gtggggctcg gaggccgcgc
     6361 acgtgccgat gaaggagcac accgccgacc tggccatggc ggccgagcgc ctgctggtgg
     6421 acagccagcg agcgggacgg ggggacctgg tggtgctggt gtcgggccgc ccgggcgggc
     6481 agggcggcac caaccggatc atggtgcacc gcgtgggtga cccgatctcc ggctgagcgg
     6541 ctcaggcgga ggagggcgcc tcgccgttgc tgccgggctc acgaagccac gacggcaccg
     6601 cgccgcggat gcggcgcagg agcgtgacca gctgctcctg ctcgtccggc gacaggtcgg
     6661 cgacgaggtt ggactgcagg cggtaatggt gcgggaagtg gctgtccagc agctcgtggc
     6721 ccgcgtcggt gagcac
//
LOCUS       DATMKC010000437         2406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_157754, whole genome shotgun sequence.
ACCESSION   DATMKC010000437 DATMKC010000000
VERSION     DATMKC010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157754"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VNU01_12685"
     CDS             <1..330
                     /locus_tag="VNU01_12685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWH33518.1"
                     /translation="GVATGGGFASWAVGAALLGAGTAMAYPTLLAAVGDVAAPAWRGA
                     AVGVYRLWRDIGFALGAVIAGVLSDAFSPRVAILAVAAITAASGADVAVRMAETHPRA
                     TPVPLRD"
     gene            complement(327..1274)
                     /locus_tag="VNU01_12690"
     CDS             complement(327..1274)
                     /locus_tag="VNU01_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HWH33519.1"
                     /translation="MSWSDLYRQAGARHGVVTLDDARAAGVPATTLRHRAAAEEWPVL
                     HRGVWALPGAPDTPLRAGAAALAAVGERGVLARRSAAWMWGLRAHPPVRPEVLFAHAR
                     GATAHDGLVALRSRSLRDVDRTDRQGLRVTTPARTLGDLAAVTSFERLAETVVRARQR
                     GLVELPDLAAQVAVMRKARGIQRLREAVVLLDGERVDSMLEHDIRAGLANARLPAPAR
                     EPIWLGSGPGRLQIDIAWPELRVGIEVDGFAWHHSAEDLARDHRKGNVAAAAGWILLR
                     VGYLRWRTDRAGFLAEARTVLSARNPAAAGPLVAGIGHN"
     gene            complement(1401..1772)
                     /locus_tag="VNU01_12695"
     CDS             complement(1401..1772)
                     /locus_tag="VNU01_12695"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HWH33520.1"
                     /translation="MPGLRGFLTLLGMVALVAGLVTVLTGASTILDANEASPSVDSEL
                     RFFAAWYVGVGVALLRAARDPAANRGIVHGVAALFGLAATGRVLSWATVGRPSTLAVV
                     LLFVEYAIALGLPLWHRRATP"
     gene            complement(1775..>2406)
                     /locus_tag="VNU01_12700"
     CDS             complement(1775..>2406)
                     /locus_tag="VNU01_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWH33521.1"
                     /translation="EREAAHLLLDTLTPIAKSWPSQWCLEANSLAIQIHGGYGYTREY
                     DVEQHYRDNRLNPIHEGTHGIQALDLLGRKVVMHGGAGLARLSGAIGETIGRARGAGG
                     EAAELGAALQAAVERVGAVTMALWGPGDVDLALANASVYLEAVGHVVVAWLWLEQFLA
                     AAGKDGEFYDGKRQATRYFYRWELPKTGPQFDLLESLDRTTLDMRDAWF"
BASE COUNT          308 a          901 c          908 g          289 t
ORIGIN      
        1 ggggtggcga cgggcggcgg tttcgcgagc tgggcggtgg gcgcggcgct gctcggggcg
       61 ggcaccgcga tggcctaccc gacgctgctg gcggccgtgg gcgacgtggc agcgccggcg
      121 tggcgcggcg ccgccgtggg cgtgtaccgg ctgtggcgcg acatcggctt cgcgctgggc
      181 gcggtgatcg cgggggtgct gtcggacgcg ttctcgccgc gggtggcgat cctggcggta
      241 gcggcgatca cggctgccag cggcgccgac gtcgcggtgc gcatggccga gacccaccca
      301 cgcgccaccc cagttccgct gcgggactag ttgtgtccga taccggccac tagtggtccc
      361 gcagcggcgg ggttgcgggc gctgaggacc gtgcgcgcct cggcgaggaa gccggcgcgg
      421 tcggtgcgcc agcgcaggta gccgacgcgc agcaggatcc agccggccgc cgccgccacg
      481 ttgcccttgc ggtggtcgcg cgccaggtcc tcggcgctgt ggtgccaggc gaagccgtcg
      541 acctcgatgc ccacgcgcag ttccggccag gcgatgtcga tctgcaggcg cccgggccct
      601 gagcccagcc agatcggttc gcgcgccggg gcgggcaggc gagcgttggc caggcccgcc
      661 cggatgtcgt gctccagcat cgagtcgacg cgctcgccgt ccagcaggac caccgcctcg
      721 cgcagccgct ggatcccgcg cgccttgcgc atgacggcga cctgggcggc gagatcggga
      781 agctcgacga ggcctcgctg gcgcgcgcgc accacggtct cggcaagccg ctcgaaggac
      841 gtcaccgcgg ccaggtcccc cagcgtccgc gccggcgtgg tgacgcgcag gccctgccgg
      901 tctgtgcggt cgacgtcgcg caggctcctg ctgcgcagcg ccaccaggcc gtcgtgggcg
      961 gtggcgccgc gcgcatgggc gaacagcacc tccgggcgca ccggcgggtg cgcccggagc
     1021 ccccacatcc acgctgcgga gcgccgcgcc agcacacccc gctccccgac cgccgcgagc
     1081 gcggcggcgc cggcccgcag cggcgtgtcc ggcgcgccgg gcagcgccca cacgccgcgg
     1141 tgcagcacgg gccactcctc cgcggcggca cgatgccgga gcgtggtggc gggcacgccg
     1201 gcggcccgcg cgtcatcgag cgtgacgacg ccgtggcggg cgcccgcctg cctgtagaga
     1261 tccgaccagg acatcggcac accatggcgc aggttcggcg acgccaggca aaaacgggcc
     1321 ggccgcctgt ggacgcccgc tccgggacga gttgtgtccg atagcggcca caagtggtcc
     1381 cgcagcgaag gccgggacgg tcagggggtg gcgcggcggt gccacagcgg caggccgagc
     1441 gcgatggcgt actccacgaa cagcagcacg acggccagcg tgctcggcct gcccaccgtc
     1501 gcccagctga gcacccgccc ggtcgcggcc agcccgaaca gcgccgcgac gccgtgcacg
     1561 atgccgcgat tggcggcggg gtcccgggca gcgcgcagca gcgccacgcc gacgcccacg
     1621 taccaggcgg cgaagaagcg cagctccgag tcgacgctgg gcgacgcctc gttggcgtcg
     1681 aggatcgtcg acgcaccggt gagcaccgtg accaggccgg cgaccagcgc caccatgccg
     1741 agcagcgtga ggaagccccg cagtcccggc atgcctagaa ccaggcgtcg cgcatgtcga
     1801 gggtggtgcg gtccagcgac tccagcaggt cgaactgcgg cccggtcttc ggcagctccc
     1861 agcggtagaa gtaccgggtc gcctggcgtt tgccgtcgta gaactcgccg tccttgccgg
     1921 cggcggcgag gaactgctcc agccacagcc acgcgaccac cacgtggccg accgcctcca
     1981 ggtacaccga ggcgttcgcc agcgcgaggt cgacgtcgcc cggcccccac agcgccatgg
     2041 tgaccgcgcc gacgcgctcc accgctgcct gaagcgccgc gccgagctcg gccgcctcgc
     2101 ccccggcgcc gcgagcgcgc ccgatggtct cgccgatcgc gccggacagg cgcgccagcc
     2161 ccgcgccgcc gtgcatcacg accttgcggc ccagcaggtc cagcgcctgg atgccgtgcg
     2221 tgccctcgtg gatggggttg aggcggttgt cgcggtagtg ctgctcgacg tcgtactcgc
     2281 gcgtgtagcc gtagccgccg tggatctgga tcgccagcga gttcgcctcc aggcaccact
     2341 gcgacggcca gctcttggcg atcggcgtca gcgtgtccag cagcaggtgc gcggcctcgc
     2401 gctcct
//
LOCUS       DATMKC010000438         2131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_170433, whole genome shotgun sequence.
ACCESSION   DATMKC010000438 DATMKC010000000
VERSION     DATMKC010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170433"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VNU01_12705"
     CDS             complement(<1..206)
                     /locus_tag="VNU01_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWH33522.1"
                     /translation="MNATGEGIHVWDTEGRRYLDFLCGLAVTSLGHAHPGVADAVAAQ
                     ARTLVHVSNLYLTEPMVTLAERLQG"
     gene            complement(203..1186)
                     /gene="argB"
                     /locus_tag="VNU01_12710"
     CDS             complement(203..1186)
                     /gene="argB"
                     /locus_tag="VNU01_12710"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWH33523.1"
                     /translation="MSGDTTTDTAIEIVEVTGRTKAAQDKARILREALPWIRHWSGRT
                     VVVKYGGNALPDEDSEDTLARAFAADVALLHSVGLRVVVVHGGGPQISALGQRLGLEP
                     RFVDGRRVTDDATLEVVKMALLGQVNPRLVGLVNAAGAPAVGVSGTDGGTVLARLLDP
                     ALGAVGAVEHVDPHLLLSLLDDNVVPVVATLGLGPDGQVLNINADTVAGAIAVALGAD
                     KLIYLSNVPGLYEYFGTEDQSLLPEVTVARLREMLDDGELATGMLPKVESIVGALNGG
                     VAQAHMLDGRIEHALLLEIFTDQGIGTMVVAEPTTTARGGIGTMVVREDGA"
     gene            complement(1183..>2131)
                     /gene="argJ"
                     /locus_tag="VNU01_12715"
     CDS             complement(1183..>2131)
                     /gene="argJ"
                     /locus_tag="VNU01_12715"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWH33524.1"
                     /translation="VCTGPDGMALAEESAALVAKELGCAVEDVLLCSTGVIGQPIPRE
                     PFLAGIPQAAAALSVDGGSRAAKAIMTTDLVAKEAAYRAEDHTGSCVVGGMVKGSGMI
                     APELATMLCVITTDAPVQGPILKATLKDAVGHTFNRISVDGCMSTNDAVIVLATGTAP
                     RPPSLSAFKEALTAVCADLAEQMVRDGEGAKKVLHIRVTGARTEADAVGVGRAIATSA
                     LVKTALAGGDPNWGRILAAMGAGPVRFEPERVVVSFGGVTVCRFGVAASFDRGVAAQQ
                     LKGRDVQLTVDLGMGDAEAVFLTCDFTSDYVRINAEYTT"
BASE COUNT          275 a          789 c          768 g          299 t
ORIGIN      
        1 ccctggaggc gctcggcgag cgtgaccatc ggctcggtca ggtacaggtt ggacacgtgc
       61 acgagcgtgc gcgcctgcgc cgccacggcg tcggcgacgc cgggatgggc gtggccgagc
      121 gacgtgaccg ccagcccgca caggaagtcg aggtagcgcc ggccctcggt gtcccacacg
      181 tggatgccct cgccggtcgc gttcatgccc cgtcctcccg caccaccatg gtgccgatgc
      241 ccccccgcgc cgtggtcgtg ggctccgcca cgaccatggt gccgatcccc tggtcggtga
      301 agatctccag gagcagggcg tgctcgatgc gcccgtccag catgtgcgcc tgggccaccc
      361 cgccgttgag cgcgccgacg atgctctcga ccttcggcag catgccggtg gcgagctccc
      421 cgtcgtcgag catctcgcgc aggcgcgcca ccgtaacctc cggcagcagg ctctggtcct
      481 ccgtgccgaa gtactcgtac aggccgggca cgttggacag gtagatcagc ttgtcggcgc
      541 cgagcgccac ggcgatggcc ccggccaccg tgtcggcgtt gatgttcagg acctgcccgt
      601 ccgggcccag cccgagggtc gccaccaccg gcaccacgtt gtcgtccagc agcgacagca
      661 gcaggtgcgg gtcgacgtgc tccacggcgc cgaccgcgcc gagcgccggg tccagcagcc
      721 gcgcgaggac cgtgccgccg tccgtgccgg acacgcccac ggccggtgcg ccggcggcgt
      781 tgacgaggcc gaccagccgc gggttcacct gcccgagcag cgccatcttc accacctcga
      841 gggtggcgtc gtcggtgacg cggcgcccgt ccacgaacct gggctccagg cccaggcgct
      901 gccccagcgc ggagatctgc gggccgccgc cgtgcaccac gacgacgcgc aggccgacgc
      961 tgtgcagcag cgcgacgtcg gcggcgaagg cgcgcgccag cgtgtcctcg ctgtcctcgt
     1021 ccggcagcgc gttgccgccg tacttcacca ccacggtgcg ccccgaccag tggcggatcc
     1081 acggcagcgc ctcgcgcagg atccgggcct tgtcctgcgc ggccttggtg cggccggtga
     1141 cctccacgat ctcgatcgcg gtgtccgtcg tcgtgtcgcc gctcatgtcg tgtactccgc
     1201 gttgatgcgg acatagtcgg aggtgaagtc gcaggtgagg aagaccgcct cggcgtcgcc
     1261 catgcccagg tcgacggtga gctgcacgtc gcggcccttg agctgctgcg ccgcgacgcc
     1321 gcggtcgaac gaggccgcca cgccgaagcg gcacacggtg acgccgccga agctcacgac
     1381 gacgcgctcg ggctcgaagc gcaccgggcc ggcgcccatc gccgccagga tccggcccca
     1441 gttcgggtcg ccgccggcca gggcggtctt gaccagggcg ctggtggcga tggcgcgtcc
     1501 cacgccgacc gcgtccgcct cggtgcgcgc gccggtgacg cggatgtgca ggaccttctt
     1561 ggcgccctcg ccgtcgcgca ccatctgctc ggccaggtct gcgcacaccg cggtgagcgc
     1621 ctccttgaag gccgacagcg acggcggccg cggcgcggtg ccggtggcca gcacgatcac
     1681 ggcgtcgttg gtggacatgc agccatccac ggagatgcgg ttgaaggtgt ggccgaccgc
     1741 gtccttcagg gtggccttga ggatggggcc ctgcacgggc gcgtccgtgg tgatgacgca
     1801 gagcatcgtg gccagctccg gggcgatcat gccgctgccc ttgaccatgc cgcccacgac
     1861 gcaggagccg gtgtggtcct cggcgcggta ggccgcttcc ttggcgacca ggtcggtggt
     1921 catgatcgcc ttggcggccc ggctgccgcc gtccaccgac agcgcggcgg cggcctgggg
     1981 tatgcccgcc aggaacggct cgcgcgggat cggctggccg atgacgccgg tggagcacag
     2041 caggacgtcc tcgacggcgc agccgagctc cttggcgacc agcgcggccg actcctcggc
     2101 cagcgccatg ccgtcggggc cggtgcagac g
//
LOCUS       DATMKC010000439         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_250559, whole genome shotgun sequence.
ACCESSION   DATMKC010000439 DATMKC010000000
VERSION     DATMKC010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250559"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VNU01_12720"
     CDS             <1..702
                     /locus_tag="VNU01_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33525.1"
                     /translation="GLADIRVFSVVPDGDGRGWTTRMLHIFTLDPLTQAVNGEIIVGE
                     AHSAVTCGAQAPGAGGENEVIIEQEVNPADPALGEDVAYDIVVRNQGTQNCTVYEVTD
                     RLAPVLTFSSAGGFLADVEPEVDGRTVLFSNPAGWPLAPGESLAGTVTATVASDATPG
                     VYTNVVVARTTCGVFTGIGAPITIGAAAPSAPGPIGASTVPAGPLPATGAGTGLAALG
                     LLGTAGGAAALLRRR"
     gene            796..1299
                     /locus_tag="VNU01_12725"
     CDS             796..1299
                     /locus_tag="VNU01_12725"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HWH33526.1"
                     /translation="MTLAAPALAPACPSCGRGAATPSRAPRLFDPGPADGAARVTTPE
                     DLAELVVPPLTGLDREHCLLVSLDTKHHVLAVTTVSIGSAAHTFMEPREVFRDALLAG
                     AVAVAVAHNHPSGDPDPSPEDRAVTRRLARAGEVLGVALLDHLVVGRSRWRSLARTEP
                     RLLRPGG"
     gene            1425..>2592
                     /locus_tag="VNU01_12730"
     CDS             1425..>2592
                     /locus_tag="VNU01_12730"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HWH33527.1"
                     /translation="MGQSIPALRRICTPLLTLAALLVALVAPASAAPRPATSLGADSF
                     GVSVSATVLGLLPVEVGPLPKVNLYLPPQAAAQRQAVVDLPPVPGLVDRAAVLEATGD
                     GDLAAGKVVASAETAGLSLLGGQIKADVLKAVSTTDCNAAGATAATASAGSRIVGLEI
                     GGQAIELPAAPGELQVRLDLPGLGGLADIRVYDVVPDADGYGWTTRMLHVFLLDPLTK
                     LVNAEIVVSEAHSTATCGEPRSDGLDNPVVISKRAVPGEVAPGREVAYDISVANASAQ
                     PCRVFKVTDRLPPGFSYVGTSGTLADVEPSVAGREVTWHNLAGWLVEPGAALTGRLTV
                     RVGAEVAPGTYFNDVIVHSTCGKFEKGQDGPVRVVAPAAPAPAPPAPQAPPPATD"
BASE COUNT          307 a         1007 c          945 g          333 t
ORIGIN      
        1 ggcctggctg acatccgcgt gttcagcgtg gtgccggatg gcgacggccg cggctggacc
       61 acgcgcatgc tgcacatctt cacgctggac ccgctgaccc aggcggtcaa cggcgagatc
      121 atcgtcggcg aggcgcactc cgcggtgacg tgcggcgccc aggcccccgg cgccggaggc
      181 gagaacgagg tcatcatcga gcaggaggtc aacccggccg atccggcgct gggggaggac
      241 gtcgcctacg acatcgtcgt gcgcaaccag ggcacgcaga actgcacggt gtacgaggtg
      301 accgaccgtc tcgcgccggt gctgacgttc tcctcagccg gcggcttcct ggccgacgtg
      361 gagcccgagg tcgacggccg caccgtgctg ttctccaacc cggccggatg gccgctggcg
      421 cccggcgagt cgctcgcggg cacggtgacc gcgaccgtcg cgtccgatgc cacgccgggc
      481 gtctacacca acgtggtcgt ggcccgcacc acgtgcggcg tgttcaccgg catcggcgcc
      541 cccatcacga tcggcgcggc cgcgccgtcc gcgccggggc cgatcggtgc gtcgaccgtc
      601 cccgcgggtc cgctgcccgc gaccggcgcc ggcaccggcc tggccgcgct gggcctgctc
      661 gggaccgccg gcggcgccgc ggcgctgctc cgccgccgct aggcacgccc gctcggctga
      721 ggagcgggcc ggtatccaca gcggcaccgg cccgatcacg cgtcgaaccg tcacggcccg
      781 ccagaatggc gggccgtgac ccttgccgcc cccgcgctcg cgccggcctg cccgtcctgc
      841 ggccggggcg ccgccacgcc gtcgcgcgcg ccccggctgt tcgacccggg cccggccgac
      901 ggcgcggcgc gcgtgaccac gccggaggac ctggccgagc tggtggtccc gccactgacc
      961 ggcctggacc gcgagcactg cctcctggtg agcctggaca ccaagcacca cgtcctcgcc
     1021 gtgaccaccg tgtcgatcgg ttcggcggcg cacacgttca tggagccgcg cgaggtgttc
     1081 cgcgacgcgc tgctggcggg cgcggtcgcc gtcgccgtgg cgcacaacca ccccagcggc
     1141 gatcccgatc ccagcccgga ggaccgcgcc gtcacccggc ggctcgcgcg cgccggcgag
     1201 gtgctcggcg tcgcgctgct ggaccatctc gtcgtcggcc gctcgcgatg gcgctcgctg
     1261 gcgcgcacgg agccgcgcct cctccgaccg gggggctgac gcatcccccg aggcgtatcc
     1321 aatgggcgag gtccagcgct gtcggtgcag acggtgggcg ggcacgccac cggtcgtctc
     1381 aggcgggcgc cgctggtcgg cggcgtgacg aaaggagccc tctcatgggc cagtccatcc
     1441 ctgcgctccg gcgcatctgc acgcccctgc tcaccttggc cgcgctgctc gtcgcgctcg
     1501 tggcgccggc gtccgcggca ccgcggcccg ccacctcgct gggcgccgac tccttcggcg
     1561 tgtcggtgag cgccaccgtg ctgggactgc tgccggtcga ggtcggccca ctgccgaagg
     1621 tgaacctgta cctgccgccg caggcggccg cgcagcgcca ggccgtcgtc gacctgccgc
     1681 cggtgccggg cctggtcgac cgtgccgccg tcctcgaggc cacgggcgac ggcgacctgg
     1741 ccgccggcaa ggtggtcgcc tccgccgaga ccgccgggct gtccctgctc ggcgggcaga
     1801 tcaaggccga cgtcctgaag gcggtgtcca cgaccgactg caacgcggcc ggcgccaccg
     1861 cggcgaccgc ctccgcgggc tcgcggatcg tgggcctgga gatcggcggc caggccatcg
     1921 agctgccggc ggctcccggt gagcttcagg tgcggctgga cctgcccggc ctgggcggtc
     1981 tcgcggacat ccgggtgtac gacgtcgtgc cggacgcgga cggctacggc tggaccacgc
     2041 gcatgctcca cgtgttcctg ctggacccgc tcacgaagct ggtcaacgcc gagatcgtgg
     2101 tcagcgaggc ccacagcacc gccacgtgcg gcgagccgcg cagcgacggg ctcgacaacc
     2161 ccgtcgtgat cagcaagcgc gccgtgcccg gcgaggtggc tcccgggcgg gaggtcgcct
     2221 acgacatcag cgtggcgaac gcgtcggcgc agccgtgccg cgtcttcaag gtgaccgacc
     2281 ggctgccccc aggcttctcc tacgtcggca cgtcggggac gctggccgac gtggagccgt
     2341 cggtcgctgg cagggaggtg acgtggcaca acctcgcggg ttggctcgtc gagccgggcg
     2401 ccgcgctcac cggccgcctc actgtccgcg tcggcgccga ggtcgcgccg ggcacgtact
     2461 tcaacgacgt gatcgtccac tcgacctgcg gcaagttcga gaagggacag gacggtccgg
     2521 tccgcgtcgt cgcgccggct gcgcccgcgc ccgcgccccc cgcaccgcag gcgccaccgc
     2581 ccgcgaccga cg
//
LOCUS       DATMKC010000440         4709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_323284, whole genome shotgun sequence.
ACCESSION   DATMKC010000440 DATMKC010000000
VERSION     DATMKC010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323284"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VNU01_12735"
     CDS             <1..799
                     /locus_tag="VNU01_12735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWH33528.1"
                     /translation="PMGESADPNPRFLADKLVDMSAMVMGQTAENLHDLYPAITRERA
                     DAYGLQSQERVQKAIANGDFDDHVVRMTVWTDEGWQVVARDEHPRPGTTMESLASLKT
                     PFRTGGRVTAGNAAGLNDGAGGVILASRETAEELGLPARMRLVDYAFVGVEPETMGYG
                     PVPATEKLFAKTGLTIDDIDVVEMNEAFAVQCIAFLDHFGLPLDSEKVNPYGGAIAIG
                     HPLAMTGARLTQQLAHHFGRNPDARYGITTLCVGLGMGATVLWENLA"
     gene            796..2904
                     /locus_tag="VNU01_12740"
     CDS             796..2904
                     /locus_tag="VNU01_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWH33529.1"
                     /translation="MSDTTAARPEAGPDEVVTEFKVTYFDSQRAGRLAIVTMDNGHDY
                     TRPNTFGPGALASIHAALDEIESQSDVKGVLLTGKQFIFAVGADLNTFTGATPELGRL
                     GAKSGHEAFARLKALPVPTVAAINGAVMGGGFEIALHCDYRTLSKGAAAIAFPEVFLS
                     IFPAWGGTQLAPVIAGAENALKVIVFNALNQNKMLKPAEAFEMGFADRLFDSVDFLDD
                     SLEFLEGVVTGEITVEREDKTNPANLDELLANARKFADDKVHGATRAPYVAIDLIEFA
                     ARGGDLAEGYRREQDGLAELLPSRQAQASVYAFNLVQQRVKKQPWKPDAKPRGVGKVA
                     VIGSGLMGAQLGALFLQSYEVALVMKDIDEAVLGRARAHIEGELDKKVQRGRLSVGKA
                     EFLKSLVTYTLDYEQVRGADFVIEAVVEILDLKKKIFADCEQVLDEGAILATNTSSLS
                     VTQMGADLAHPERVVGFHFFNPVAVMPLLEVVRTEVSSDEAMATAFAVAKKLKKSAVQ
                     CADTPAFVVNRLLTRFMGACATAVNKGNDFAEVDDAVKELGLPMGPFELLGLVGLAVA
                     AHVGETMHHAYPERFPLDENMQMLGKTGLPGIYDWSKGRVPYEEILQAWSPEPGAQKY
                     TAEEIRQVALEAVADEIRHMLDEGVVADARDIDTCMLLGAGWPFFMGGICKYLDQTGM
                     SEKLFGAPLIGETDRAFASA"
     gene            complement(2943..3287)
                     /locus_tag="VNU01_12745"
     CDS             complement(2943..3287)
                     /locus_tag="VNU01_12745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWH33530.1"
                     /translation="MAEWRRMMRRGEIRWANLPPPLYRRPVCIVTRDSALPWLTTVLC
                     APLTTRVRGIDSEVPVPGHIRLDRPTVINCDSLRPVRVDDSDDHSLGALDLASLRALD
                     AALIYTLGILAA"
     gene            complement(3266..3517)
                     /locus_tag="VNU01_12750"
     CDS             complement(3266..3517)
                     /locus_tag="VNU01_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWH33531.1"
                     /translation="MLVTKRRVEIELDEEQLARLDWLCRRTGKTRQELLQEGVRWVFR
                     TNDGPWDLDDPLEQAALDWLQARAVAEFDRTDPWPSGGE"
     gene            complement(3627..>4709)
                     /locus_tag="VNU01_12755"
     CDS             complement(3627..>4709)
                     /locus_tag="VNU01_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33532.1"
                     /translation="VFVSVDTPQNSAECGSGSAGVPTYASGQAWEGIRVVSIANPTKP
                     EQIAAVDTDCGSHTHTLLPDIDHVDEDGRPDPRVLLYVSSYPLGGQGVECNAAGHRKI
                     SVVEVPLRRPAEASVLSTPDVSPAVGCHDITVFPELGLAAAACISESQLWDIRDPANP
                     QIVSHIRNPRMQIHHSSAFSWDGEVLVLGDEMGGAAAAAGCLAGGHAPTGSLWFYDVS
                     DPANPVEQGWFTIPQDQPSMQCTAHNFNVVPTVDGSRVLVTAWYNGGTHVLDFDDPAN
                     VEQLAYAKPAHGSSWSSYWYNGHVYVNNFDEGYVPPIAYSRGFDVMTVDDGLLPESHD
                     LPHLNPQTQLRPPKAEPKAQPGSGRR"
BASE COUNT          748 a         1591 c         1692 g          678 t
ORIGIN      
        1 tcccatgggc gagtccgccg acccgaaccc gcggtttttg gcagacaagc tggtcgacat
       61 gtcggcgatg gtcatgggcc agaccgccga gaacctgcac gacctgtacc cggcgatcac
      121 acgcgagcgc gccgacgcct acgggctgca gtcccaggag cgcgtgcaga aggccatcgc
      181 caacggcgac ttcgacgacc acgtcgtgcg catgacggtg tggaccgacg agggctggca
      241 ggtggtggcc cgcgacgagc acccgcgtcc cggcaccacc atggagagcc tggcgtcgct
      301 caagacgccg ttccgcaccg gcgggcgcgt cacggcgggc aacgcggccg gcctcaacga
      361 cggcgccggg ggcgtgatcc tcgcctcgcg cgagacggcc gaggagctgg gccttccggc
      421 ccgcatgcgc ctggtcgact acgccttcgt gggcgtggag ccggagacga tgggctacgg
      481 cccggtgccc gcgacggaga agctcttcgc caagacgggg ctgaccatcg acgacatcga
      541 cgtcgtcgag atgaacgagg cgttcgcggt gcagtgcatc gcgttcctgg accacttcgg
      601 cctgccgctg gactccgaga aggtgaaccc gtacggcggc gcgatcgcca tcggccaccc
      661 gctggccatg accggcgcgc gcctcaccca gcagctcgcg caccacttcg gccgcaaccc
      721 cgacgcccgc tacggcatca ccacgctgtg cgtgggcctg ggcatgggcg ccaccgtcct
      781 ctgggagaac ttggcatgag cgacacgacc gctgcccggc ccgaggccgg ccccgacgag
      841 gtcgtgaccg agttcaaggt cacctacttc gacagccaga gggccggccg cctcgccatc
      901 gtgacgatgg acaacggcca tgactacacc cggccgaaca cgttcggccc cggcgcgctg
      961 gcgtccatcc acgccgccct ggacgagatc gagtcccagt ccgacgtgaa gggcgtgctg
     1021 ctgaccggca agcagttcat cttcgccgtc ggcgcggacc tcaacacgtt caccggcgcg
     1081 acgccggagc tggggcgcct gggcgcgaag agcggccatg aggcgttcgc gcggctgaag
     1141 gccctgcccg tgccgaccgt cgcggcgatc aacggcgccg tcatgggcgg cgggttcgag
     1201 atcgcgctgc actgcgacta ccgcacgctg tccaagggcg ccgcggcgat cgcgttcccg
     1261 gaggtgttcc tgtcgatctt ccccgcgtgg ggcggcacgc agctcgcacc ggtgatcgcg
     1321 ggcgccgaga acgcgctcaa ggtgatcgtc ttcaacgcgc tcaaccagaa caagatgctc
     1381 aagcccgccg aggcgttcga gatgggcttc gccgaccggc tgttcgactc ggtggacttc
     1441 ctggacgact cgctggagtt cctggagggc gtggtcaccg gcgagatcac cgtggaacgc
     1501 gaggacaaga ccaacccggc caacctcgac gagctgctgg ccaacgcgcg caagttcgcc
     1561 gacgacaagg tccacggcgc cacccgcgcg ccgtacgtcg ccatcgacct catcgagttc
     1621 gccgcgcgcg gcggcgacct ggccgagggc taccggcgcg agcaggacgg tttggccgag
     1681 ctgctgccca gccggcaggc gcaggccagc gtgtacgcgt tcaacctggt gcagcagcgc
     1741 gtgaagaagc agccgtggaa gcccgacgcc aagccgcgcg gcgtcggcaa ggtcgcggtc
     1801 atcggctccg gcctgatggg cgcgcagctc ggcgcgctgt tcctgcagag ctacgaagtg
     1861 gcgctggtca tgaaggacat cgacgaggcg gtgctcggcc gggcgcgggc gcacatcgag
     1921 ggcgagctcg acaagaaggt gcagcgcggc cggctgtcgg tcggcaaggc ggagttcctc
     1981 aagtcgctgg tcacctacac gctggactac gagcaggtgc gcggcgcgga cttcgtgatc
     2041 gaggccgtgg tggagatcct ggacctcaag aagaagatct tcgccgactg cgagcaggtg
     2101 ctggacgagg gcgcgatcct ggcgaccaac acctcgtcgc tgtcggtcac gcagatgggc
     2161 gcggacctcg cgcacccgga gcgtgtcgtc ggcttccact tcttcaaccc ggtcgcggtc
     2221 atgccgctgc tggaggtggt ccgcaccgag gtgagttccg acgaggcgat ggccacggcg
     2281 ttcgccgtcg ccaagaagct caagaagtcc gcggtgcagt gcgccgacac cccggcgttc
     2341 gtggtcaacc gcctgctgac ccgcttcatg ggcgcgtgcg cgaccgccgt caacaagggc
     2401 aacgacttcg ccgaggtcga cgacgcggtg aaggagctgg ggctgccgat gggcccgttc
     2461 gagctgctcg gcctggtggg cctggccgtg gcggcgcacg tcggcgagac catgcaccac
     2521 gcctacccgg agcgcttccc gctggatgag aacatgcaga tgctcggcaa gaccggcctg
     2581 ccggggatct acgactggtc caagggccgg gtgccgtacg aggagatcct gcaggcgtgg
     2641 tcgcccgagc cgggtgcgca gaagtacacc gcggaggaga tccgccaggt ggcgctggag
     2701 gcggtggccg acgagatcag gcacatgctg gacgagggcg tggtcgcgga cgcgcgcgac
     2761 atcgacacct gcatgctgct gggcgccggc tggccgttct tcatgggcgg catctgcaag
     2821 tacctggacc agaccggcat gagcgagaag ctgttcggcg ccccgctcat cggcgagacc
     2881 gaccgggcat tcgcctccgc ctagatcacc gctgcagcat gagggtccgc gcggcccctc
     2941 actcaggccg cgaggatccc caaggtgtag atcagcgccg catccagcgc gcgcagtgag
     3001 gcaaggtcga gtgcgccgag ggagtggtcg tccgagtcat cgacgcgcac cggccgaagg
     3061 ctgtcgcagt tgatgacggt cggcctatcc agacggatgt gcccgggtac cggcacctcc
     3121 gagtcgatgc cgcgaactcg ggtcgtgagc ggtgcgcaca gcacagtcgt gagccagggc
     3181 agagcggagt cgcgcgtgac gatgcagacc gggcggcgat acagcggcgg tggaaggttc
     3241 gcccagcgga tctcgccgcg tcgcatcatt cgccgccact cggccaaggg tccgtccgat
     3301 cgaactcggc gaccgcacgc gcctgcaacc aatccagagc ggcctgctcc aacggatcgt
     3361 caaggtccca cgggccgtcg ttggttcgga agacccagcg cacgccttcc tggagcaact
     3421 cctggcgcgt cttgccggtg cgccggcaca gccagtcaag ccgggcgagt tgctcctcgt
     3481 ccagctcgat ctcgacacgc cgcttggtca ccagcacacc gtagaagctg caccgggact
     3541 tgggcagtgg caaccgacgc ggcgctatcg ctgtggacaa cgcgaggggc cgcgcgaggc
     3601 gcggcccctc gtcgggaagc cgtccgtcaa cggcggccgc tgccgggttg ggccttgggt
     3661 tcggccttgg gcggccgcag ctgcgtctgc gggttcaggt gcggcaggtc gtgggactcc
     3721 gggagcagcc cgtcgtcgac cgtcatcacg tcgaagccgc ggctgtaggc gatgggcggc
     3781 acgtagccct cgtcgaagtt gttgacgtag acgtggccgt tgtaccagta ggacgaccac
     3841 gaggagccgt gcgccggctt ggcgtaggcg agctgctcga cgttcgcggg gtcgtcgaag
     3901 tccagcacgt gggtgccgcc gttgtaccag gcggtcacca ggacgcggct gccgtccacg
     3961 gtgggcacca cgttgaagtt gtgcgcggtg cactgcatgg agggctgatc ctgcgggatg
     4021 gtgaaccagc cctgttccac cgggttggcg gggtcggaga cgtcgtagaa ccacagcgac
     4081 ccggtcggcg cgtgcccgcc cgccaggcag cccgcggccg cggcggcccc gcccatctcg
     4141 tcgccgagga ccagcacctc gccgtcccag gagaacgccg aggagtggtg gatctgcatc
     4201 ctggggttgc ggatgtggga gacgatctgg gggttggcgg ggtccctgat gtcccacagc
     4261 tgcgactcgc tgatgcacgc cgccgccgcg aggcccagtt cggggaagac cgtgatgtca
     4321 tggcagccga ccgcggggga cacgtcgggc gtggacagca cgctggcctc ggccggccgg
     4381 cgcagcggca cctcgaccac ggagatcttg cggtggccgg cggcgttgca ctccacgccc
     4441 tgcccgccca gcgggtagga ggagacgtag agcagcacgc gcggatcggg ccgcccgtcc
     4501 tcgtccacat ggtcgatgtc gggcagcagc gtatgggtgt gcgagccgca gtcggtgtcc
     4561 accgcggcga tctgctcggg cttggtcggg ttggcgatgg agacgacgcg gatgccctcc
     4621 cacgcctgcc ccgacgcgta cgtcggcacg cccgcgctgc cgctgccgca ctcggcgctg
     4681 ttctgcggcg tgtccacgga cacgaacac
//
LOCUS       DATMKC010000441         5911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_83084, whole genome shotgun sequence.
ACCESSION   DATMKC010000441 DATMKC010000000
VERSION     DATMKC010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83084"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1214
                     /locus_tag="VNU01_12760"
     CDS             42..1214
                     /locus_tag="VNU01_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWH33533.1"
                     /translation="MTIYLDHAATTPLDPRVLAGMRSWLEPAEQSPGHWGNPSSAHAY
                     GRHAREAVETAREEVARALGVTPLEVLFTSGGTEADNQAVKGIAWAARDAGRGTHLVT
                     TAIEHHAVLESVEWLARHQGFEATVVPVEPDGVVDPDRLLAAVRADTALVSVMAANNE
                     LGTVQPLDVLGPALRERGVPLHTDAVQAFGRTELRLDDWGVGAAALSAHKFNGPKGVG
                     VLVLRRDLAAQPVLHGGGQERGVRSGTLNAAGIVGCGLAAGFTVREMAEEVPRLAALR
                     DRLLDGLLAVDGVTLNGSRDRRLPHNAHVAVEGCDGEALVTALDRQGVAVSTGSACQS
                     GAAEFSHVLRAIGAPQERAHLRFTCGRGTSEQDVDAAAGIFGDCVATLRAAGGGYL"
     gene            1211..2281
                     /gene="mnmA"
                     /locus_tag="VNU01_12765"
     CDS             1211..2281
                     /gene="mnmA"
                     /locus_tag="VNU01_12765"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWH33534.1"
                     /translation="MSRVLVAMSGGVDSSMAAALLVDQGHDVAGVHLKMADTPSGLPG
                     KGCCTLDDARDARRVADLLGIPFYVWDLSEPFQERVVDDFVAEYAAGRTPNPCVRCNE
                     RVKYTALLGRARALGFDALATGHHVRLRDDGGRWRMRRAADPGKDQTYVLYMATQDQL
                     AHTLWPAGEHHKAALRDMAAERGLVTARKPDSHDICFIPSGDLGAFLRPRLGAREGVF
                     VGPGGDVVGRHDGAYRFTVGQRRGLGLAGFDEPLYVTGIEGERVHVGPRDALERSWLE
                     AFDVSWVGGEPPASPEVGAQVRYRGEALPARLHERGDGRVRVEFSRERPLGIAPGQAV
                     VFYDADECLGGATIGASGPAAD"
     gene            2342..2662
                     /gene="fdxA"
                     /locus_tag="VNU01_12770"
     CDS             2342..2662
                     /gene="fdxA"
                     /locus_tag="VNU01_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWH33535.1"
                     /translation="MVYIIAEPCIDVKDKACVEECPVDCIYEGERSLYIHPGECIDCA
                     ACEPACPVEAIFYEDDVPDEWKQFIAINAEWFSDAVTGLGDPGGAAQVGPQPKDHPAV
                     ANWS"
     gene            2760..3512
                     /locus_tag="VNU01_12775"
     CDS             2760..3512
                     /locus_tag="VNU01_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWH33536.1"
                     /translation="MGDEMTIDELARRAGVTSRNVRAYQERGLLPPPTVRGRVGYYGE
                     GHLARLRHIAQLSERGFSLASIRQLFDAWERGWGLAEVLGFEEALAAPWADESSDLVL
                     SQEQLVATFGGGDDTVQRAVELRLLEPAPEEEGFRVPSPQVLQAGSALIDAGVPLRAV
                     LDEAAALQEDMDRIAGRFVRLFLDHVWQPFVNRGMPPEELPAVTEALHRLRPVAGITV
                     THWLAAAMERCVNEVAAETLALPSTNVVARGE"
     gene            3882..4649
                     /locus_tag="VNU01_12780"
     CDS             3882..4649
                     /locus_tag="VNU01_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33537.1"
                     /translation="MKFLRLPLALAAVMTLLLVGAPASADGTHTSMDFTCADPSPNTT
                     DTPATNSCTFDFNDSGDFGTTDNDKVDYFEIVLPDGAQINEDNDQGGNGSDGVADPAD
                     GDDVGYSTVRTELSYNSCDDNNNDGGTYTTEWDNDWDSYTVPNGWTKVAEYNTIFSVL
                     FFFTRHVPSHVLVNDTTGQYKIVTDLPEADGCAGAHTKFNPLVTYAHADNDSNNTWIS
                     KTPTTGDCYTVTITATEVGGTTHTDTDVVDVGGQPGC"
     gene            4820..>5911
                     /gene="ligA"
                     /locus_tag="VNU01_12785"
     CDS             4820..>5911
                     /gene="ligA"
                     /locus_tag="VNU01_12785"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWH33538.1"
                     /translation="MNDSAQAGEDVRLRAEALRADIREHRDRYYNAQAEITDAAFDAL
                     MQELRELEARHPELVTPDSPTQQVGAPPAAAFAQVEHRLPMLSLDNAFSREELQAWAD
                     RVSRGLDGQPHRFTCELKVDGVAISLSYQHGTLARAVTRGDGRVGEDVTANVRTIRGV
                     PHELSLAKPPALLEVRGEIYFPVEDFGRMNDEREARGEPRFANPRNAASGALRQKDTR
                     VTAQRPLRVICHGMGATDGVEVTSHSGFLAYLAGAGLPVAPETRTFDDIEAVWAFVEH
                     WGEHRHDPAYELDGVVVKVDEHAQQRRLGFTSSAPRWAIAFKYPPEEQQTLLRDIAIN
                     VGRTGKVTPFAVLEPVLVAGSTISMATLHN"
BASE COUNT          855 a         2114 c         2159 g          783 t
ORIGIN      
        1 cagcgggggc cgctgcgccg ctagcgcggt agcgtcgtgg cgtgacgatc tacctggacc
       61 acgccgccac gacgccgctg gacccgcgcg tcctggcggg catgcgctcg tggctggagc
      121 cggcggagca gtcgccgggg cactggggca acccctcgtc ggcgcacgcg tacggccgcc
      181 atgcccgcga ggccgtggag accgcccgcg aggaggtggc gcgcgcgctc ggcgtgacgc
      241 ccctggaggt gctgttcacc tccggtggca ccgaggcgga caaccaggcg gtcaagggca
      301 tcgcctgggc ggcgcgcgac gccggccgcg gcacccacct ggtcaccacc gccatcgagc
      361 atcacgccgt cctggagtcg gtggagtggc tggcgcgcca ccagggcttc gaggccaccg
      421 tggtgcccgt cgagcccgac ggcgtcgtgg accccgaccg cctgctggcg gccgtgcgcg
      481 ccgacacggc gctggtctcg gtcatggcgg ccaacaacga gctgggcacg gtgcagccgc
      541 tcgacgtcct cgggcccgcg ctgcgcgagc gcggcgtgcc cctgcacacc gacgccgtgc
      601 aggcgttcgg ccgcaccgag ctgcgcctgg acgactgggg cgtcggcgcg gcggcattgt
      661 cggcgcacaa gttcaacggt cccaagggcg tcggtgtcct ggtgctgcgc cgcgaccttg
      721 ccgcccagcc ggtgctgcac ggcggcgggc aggagcgcgg cgtgcgctcc ggcacgctga
      781 acgcggccgg catcgtcggg tgcgggctgg cggccggctt cacggtgcgg gaaatggccg
      841 aggaggtgcc gcgcctcgcg gcgctgcgcg accgcctgct cgacgggctg ctcgccgtgg
      901 acggcgtcac actcaacggc tcgcgcgacc ggcgcctgcc gcacaacgcc cacgtcgccg
      961 tggagggctg cgacggcgag gcgctcgtca ccgccctgga tcgccagggt gtcgcggtga
     1021 gcacgggctc ggcgtgccag tcgggcgcgg cggagttcag ccacgtcctg cgcgcgatcg
     1081 gcgcgccgca ggaacgcgcg cacctgcgct tcacctgcgg gcgcggcacc tccgagcagg
     1141 acgtcgacgc cgcggccggc atcttcggcg actgcgtcgc aacgctgcga gccgcgggcg
     1201 ggggctacct gtgagccgtg tgctggtggc catgtcgggc ggcgtggaca gctccatggc
     1261 cgccgcgttg ctggtggatc agggccacga cgtcgccggc gtgcacctga agatggccga
     1321 cacgccgtcg gggctgcccg gcaagggctg ctgcaccctg gacgacgcgc gggacgcacg
     1381 gcgcgtggcc gacctgctcg gcatcccgtt ctacgtctgg gacctgtccg agccgttcca
     1441 ggagcgcgtg gtcgacgact tcgtggcgga gtacgcggcc ggccgcacgc ccaacccctg
     1501 cgtgcgctgc aacgagcgcg tgaagtacac ggcgctgctg gggcgggcgc gggcgctcgg
     1561 gttcgacgcg ctggccaccg gccaccacgt ccgcctgcgc gacgacgggg gccggtggcg
     1621 catgcgccgc gccgccgacc ccggcaagga ccagacctac gtgctgtaca tggccacgca
     1681 ggaccagctc gcgcacaccc tgtggcccgc gggcgagcac cacaaggccg cgctgcgcga
     1741 catggcggcc gaacgcgggc tcgtgacggc gcgcaagccg gacagccacg acatctgctt
     1801 catcccctcc ggcgacctcg gcgccttcct gcgcccgcgg ctgggcgccc gcgagggcgt
     1861 gttcgtgggg ccgggcgggg atgtcgtcgg ccgccacgac ggcgcctacc gcttcaccgt
     1921 ggggcagcgc cgcgggctgg gcctggccgg cttcgacgag ccgctgtatg tcaccggcat
     1981 cgagggggag cgcgtgcacg tgggcccgcg cgacgcgctg gagcgctcgt ggctggaggc
     2041 cttcgacgtc tcctgggtgg gcggcgagcc gccggcgtcg cccgaggtcg gcgcgcaggt
     2101 gcgctaccgg ggcgaggcgc tgcccgcgcg cctgcacgag cgcggggacg ggcgcgtgcg
     2161 cgtggagttc tcccgtgaac gtccgctcgg gattgcgccc ggacaggctg tggtgttcta
     2221 cgatgccgac gagtgtctcg gcggcgccac cattggtgcc tccgggccag cggcggactg
     2281 agcgtgcagg acgccgggta gcgcgccggg ccaggacgaa ggattcgagg agccgtcaaa
     2341 gatggtctac atcatcgccg agccgtgcat cgacgtgaag gacaaggcct gcgtcgagga
     2401 gtgccccgtc gactgcatct acgagggtga gcgctcgctg tacatccacc ccggtgagtg
     2461 catcgactgc gccgcctgcg agccggcgtg ccccgtggag gcgatcttct acgaggacga
     2521 cgtccccgac gagtggaagc agttcatcgc gatcaacgcg gagtggttct ccgacgcggt
     2581 gaccggcctc ggggaccccg gcggcgcggc gcaggtcggc ccgcagccca aggaccaccc
     2641 ggccgtcgcc aactggagct gaggcccgcg ccgcaccgtt tccgacgagc cccgccgttg
     2701 tggcggggct cgtcgcatct gcggcaggtt ggccacgtcg agtgctaccg tcggtgccga
     2761 tgggcgacga gatgacgatc gacgagctcg cgcgcagggc gggggtcacc agccgcaacg
     2821 tccgggccta ccaggagcgc ggcctgctgc cgccgccgac cgtgcgcggc cgggtgggct
     2881 actacggcga gggccacctg gcccggctgc gccacatcgc ccagctgtcc gagcgcggct
     2941 tctcgctggc gtccatccgc cagctcttcg acgcctggga gcgcggctgg ggcctggccg
     3001 aggtgctcgg cttcgaggag gcgctggcgg ccccgtgggc ggacgagtcc tccgacctgg
     3061 tgctcagcca ggagcagctc gtcgccacgt tcggcggcgg tgacgacacg gtgcagcgcg
     3121 cggtggagtt gcggctgctg gagcctgccc ctgaggagga gggcttccgc gtgccgagcc
     3181 cgcaggtgct ccaggcggga tcggccttga tcgacgccgg ggtcccgctg cgcgcggtac
     3241 tggacgaggc ggcggcgctg caagaggaca tggaccgcat cgcgggccgc ttcgtcaggc
     3301 tgttcctgga ccacgtgtgg cagccgttcg tcaatcgggg catgccgccc gaggaactgc
     3361 ccgccgtcac ggaggcgctg caccggctgc ggccggtggc gggcatcacc gtgacccact
     3421 ggctcgccgc ggcgatggag cggtgcgtca acgaggtggc tgcggagacg ctcgcgctgc
     3481 cgtcgaccaa cgtggtcgcg cggggcgagt gagagagcgg gtttccccga caccgcccca
     3541 ccgctgtccg gacaagctga gcgtgcgcat acgtagcacg gcgctggggg ctgtcccgca
     3601 tgccccgtgc gtcttcgcgg aggcgaactt ccgtagacac acggaaaatc tcgaaacacc
     3661 tggttgaccc cctcgcggaa cccgccatag aaccgtggta cgtgatccgg tggctttcac
     3721 agcaggcgac aacatcgccg ttcgcacccg ccgcggatcg gggggatcgt cgcgacgcca
     3781 cgccggctga tccagccgtg cgccacaggc cctcctcgcc gcgcaccacg ctgcctgcgt
     3841 tcgcgtgacc tgttcgagcc caaccacacg aggagtccga aatgaagttt ctcagactgc
     3901 cgctggccct ggccgcggtc atgaccttgc tgctcgtcgg cgcgcccgca tcggccgacg
     3961 gcacacacac gtcgatggat ttcacctgcg cggacccgag tccgaacacg acggacacgc
     4021 ccgcgacgaa cagttgcacg ttcgatttca acgactctgg tgattttggc actaccgaca
     4081 acgacaaggt cgactacttc gagatcgtcc tgccggacgg cgcgcagatc aatgaggaca
     4141 acgaccaagg tggcaacggc tcggacgggg tcgcggatcc cgcggacggc gacgacgtgg
     4201 gctacagcac ggtccggacc gaactgtcct acaacagctg tgacgacaac aacaacgacg
     4261 ggggcaccta caccaccgag tgggacaacg actgggacag ctacaccgtc ccgaacggct
     4321 ggaccaaggt ggcggagtac aacaccatct tcagtgtgct gttcttcttc acccggcacg
     4381 tgccgtccca cgtgctcgtc aacgacacga ccgggcagta caagatcgtg accgacctgc
     4441 ccgaggcgga cggctgcgcc ggcgcgcaca ccaagttcaa cccgctcgtt acgtacgccc
     4501 acgccgacaa cgactccaac aacacctgga tcagcaagac ccccacaacg ggcgactgct
     4561 acacggtgac gatcaccgcg accgaggtcg gtggcaccac gcacaccgat acggacgtgg
     4621 tcgatgtcgg gggtcagccc ggctgctgac ggtggcgggg gcggtcgtca tgctcgtgcc
     4681 gggcatggag gtcggcggca ccgcccgcag ggacaccgtg acagtccgct gacgggcgca
     4741 tgcccgcccg tcggcgccgc cgccccggtc ccttcggggc ggcggtccac tgtcgcggcg
     4801 gctgcataga ctcccgcgca tgaacgacag tgcccaggcc ggcgaggacg tgcgcctgcg
     4861 cgcggaggcg ctccgcgcgg acatccgcga gcaccgggac cggtactaca acgcgcaagc
     4921 cgagatcacc gacgcggcgt tcgacgcgct catgcaggag ctgcgcgagc tggaggcgcg
     4981 ccacccggag ctggtcacgc cggactcgcc cacacagcag gtgggcgccc cgcccgcggc
     5041 ggcgttcgcg caggtggagc accgcctgcc gatgctgtcg ctggacaacg cgttctcccg
     5101 ggaggaactg caggcctggg ccgaccgggt gtcgcgcggc ctggacggcc agccccaccg
     5161 cttcacctgc gagctcaagg tggacggggt cgcgatcagc ctgtcctacc agcacggcac
     5221 gctcgcgcgc gccgtgacgc gcggcgacgg gcgcgtgggg gaggacgtca cggccaacgt
     5281 ccgcaccatc cgcggcgtgc cgcacgaact gtcgctggcg aagccgcccg cgctgctgga
     5341 ggtgcgcggc gagatctact tcccggtgga ggacttcggc cgcatgaacg acgagcggga
     5401 ggcacgcggc gagccgcgct tcgccaatcc gcgcaacgcc gcgtcgggcg cgctgcgcca
     5461 gaaggacacc cgcgtcaccg cgcagcggcc gctgcgtgtc atctgccacg gcatgggcgc
     5521 caccgacggg gtcgaggtga ccagccacag cggcttcctg gcctaccttg ccggcgcggg
     5581 cctgcccgtg gcgccggaga cccggacatt cgacgacatc gaggccgtgt gggcgttcgt
     5641 cgagcactgg ggcgagcacc gccacgaccc cgcctacgag ctcgacggcg tggtcgtgaa
     5701 agtggacgag cacgcgcagc agcggcgcct cggcttcacc tccagcgccc cgcgctgggc
     5761 gatcgcgttc aagtacccgc cggaggagca gcagaccctg ctgcgcgaca tcgccatcaa
     5821 cgtcggccgc accggcaagg tgaccccgtt cgccgtgctg gagccggtgc tggtcgccgg
     5881 gtccaccatc tccatggcca ccctgcacaa c
//
LOCUS       DATMKC010000442         2982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_353555, whole genome shotgun sequence.
ACCESSION   DATMKC010000442 DATMKC010000000
VERSION     DATMKC010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353555"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..313)
                     /locus_tag="VNU01_12790"
     CDS             complement(35..313)
                     /locus_tag="VNU01_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33539.1"
                     /translation="MMQMPRSLDELMARADELAETFEAYEPHSADRGEPPLMALRRLA
                     FQRSMMERELTDAVRQARKAGTSWAKIGSELGTSGEAARQRYADKVGT"
     gene            complement(310..456)
                     /locus_tag="VNU01_12795"
     CDS             complement(310..456)
                     /locus_tag="VNU01_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33540.1"
                     /translation="MDAFAVGDGLVMYVGPDESGDLLEVGVVGWHGVLVIVHAMPARK
                     KFLR"
     gene            723..1226
                     /locus_tag="VNU01_12800"
     CDS             723..1226
                     /locus_tag="VNU01_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWH33541.1"
                     /translation="MRIIIDDLSGPEIAEFLAEHVEEMRAVTPPQSKHALDLDGLRSA
                     DVTFWTVLRDGTVIACGAIKRLGGGDAEIKSMRVAPAHRRQGVASALLEHICAAATGM
                     GLARLYLETGSFDYFAPARGLYLRHGFTYCEPFADYKPDPNSVFLTKTLSLPAGLGVA
                     QAPATSA"
     gene            complement(1202..1363)
                     /locus_tag="VNU01_12805"
     CDS             complement(1202..1363)
                     /locus_tag="VNU01_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33542.1"
                     /translation="MIVEHALLVRWARLGDHTEGFRGSAEYAQWRILLHHFYDPFPEV
                     EHYAEVAGA"
     gene            1433..1723
                     /locus_tag="VNU01_12810"
     CDS             1433..1723
                     /locus_tag="VNU01_12810"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HWH33543.1"
                     /translation="MSDEGTAPPQQPAVQITVPDDAKRGEYANFLVVTHSPHEFTLDF
                     CQVVTGTDPERMQAEVVSRVKLAPTMVGKVIRALNDNLTGYEEKFGLVKEIG"
     gene            complement(1743..2213)
                     /locus_tag="VNU01_12815"
     CDS             complement(1743..2213)
                     /locus_tag="VNU01_12815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWH33544.1"
                     /translation="MGEQQELQIGAEAPDFELRDQHGQPWRLSDYRGKKHVVVVFMPF
                     AFSRTCTGELCAIGEDLSAFQNDDTQVVAVSCDSPFALKAWAEKERYDFPMLSDFWPH
                     GAVSAQYGVFEERVGAASRSTFLVDRDGVLRWKVLNGWDARDTESYKKALAEIG"
     gene            2262..2960
                     /locus_tag="VNU01_12820"
     CDS             2262..2960
                     /locus_tag="VNU01_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33545.1"
                     /translation="MPPSADAADRGTPHAAAAGWRLLGVERGEPAALLARGLELLDGL
                     SPGDPPVLRWYVPSAPALVLGRGQRDTDVRTDLPVARRHSGGGAVLMDADLLSLDVVF
                     PAGHPMAAGDLGEAFLRVGEAWAAALGDLGVTGLEVHRGASPASRLGGPRERLLAAVC
                     YALPGRSASATAARTGRACRGGAGAAPGRPACARRAAPRRAPAARSRPRRAGARAGRP
                     GGLRGRRRGAPPVR"
BASE COUNT          434 a         1118 c         1009 g          421 t
ORIGIN      
        1 gtggcaagct gagcccacag gggccctgtc gaacctacgt gccgaccttg tcggcgtagc
       61 gctgccgcgc ggcttcaccc gaggtgccga gttcgctgcc gatcttcgcc cacgacgtgc
      121 ccgccttgcg ggcctggcgg acggcgtcgg tcagttcgcg ctccatcatg gaccgctgaa
      181 aggcgagtcg gcgcaacgcc atgagtggcg gctccccgcg gtcggcgctg tgaggctcgt
      241 acgcctcgaa cgtttcggcg agctcgtcgg cgcgcgccat gagttcgtcc aaagaccggg
      301 gcatctgcat cacctcagga acttcttgcg agccggcatc gcgtggacga tcacaagcac
      361 gccatgccag ccgacgacac caacctcgag gaggtcgcct gactcgtcag ggccgacata
      421 catcaccagg ccgtcgccaa ccgcgaacgc gtccatcgcg aacgcccaag cgtgcggggc
      481 agacgtgtcg cgaacgccgt gacggccggc gttgcaagca caaacggggg ttcttcatcc
      541 actgcaagtt acctttgctg cggggtgaac gcaaggtagc ttgtagcagc ttggatgcgt
      601 cagacttcca gcaatggccg agtggctgtg gatactccag ctctggcaca acggacggtc
      661 gtccgcctct cacggctttc acgcttaccg gcgaccgtgg gcgtcgcgcg gaggagcggg
      721 ggatgcggat catcatcgac gacttgtccg ggccggagat cgccgagttc ctggccgagc
      781 acgtcgagga gatgcgggcg gtcaccccgc cgcagagcaa gcacgcgctg gacctggacg
      841 gcctccgcag cgctgacgtc accttctgga ccgtgctgcg ggacggcacc gtgatcgcgt
      901 gcggtgcgat caagcgcctc ggcggcggcg acgcggagat caagtcgatg cgcgtcgccc
      961 cggcgcaccg ccggcagggc gtcgcgtcgg cactgcttga gcacatctgc gccgcggcca
     1021 cggggatggg tctcgcgcgc ctgtacctgg agaccgggtc attcgactac ttcgcgcccg
     1081 ccagaggcct gtacctgagg cacggcttca cgtactgcga gcccttcgcc gactacaaac
     1141 cggacccgaa cagcgtcttc ctcacgaaga cgctctcact gcccgctggg ctgggcgtcg
     1201 ctcaggcgcc cgccacctcg gcgtagtgct cgacctcggg gaacgggtcg tagaagtggt
     1261 gcagaaggat gcgccactgc gcgtactcgg cggagccgcg gaacccctcg gtgtggtccc
     1321 ccaggcgcgc ccagcgcacc agcagcgcat gctcgacgat catgcggcca tcatgcgccg
     1381 cccgtctcgc gggtagggtc aggcgacgcc gccgacccga ggaggtcctt gcatgagcga
     1441 cgaaggcacc gcgccgccgc agcagcccgc cgtccagatc accgtgcccg acgacgccaa
     1501 gcgcggcgag tacgccaact tcctggtcgt gacccactcg ccgcacgagt tcacgctcga
     1561 cttctgccag gtggtcaccg gcaccgaccc cgagcgcatg caggcggagg tggtcagccg
     1621 cgtgaagctc gcgcccacca tggtgggcaa ggtcatccgc gcgctgaacg acaacctcac
     1681 cggctacgag gagaagttcg gcctcgtgaa ggagatcggc tgaggccagg cccgcggcgc
     1741 gctcagccga tctcggcgag cgccttcttg taggactcgg tgtcgcgcgc gtcccagccg
     1801 ttgagcacct tccagcgcag cacgccgtcg cggtcgacca ggaacgtgct gcgcgacgcc
     1861 gcgccgaccc gctcctcgaa caccccgtac tgcgcggaca cggcgccgtg cggccagaag
     1921 tccgacagca tcgggaagtc gtagcgctcc ttctccgccc acgccttcag cgcgaacggc
     1981 gagtcgcagc tcaccgccac cacctgcgtg tcgtcgttct ggaacgcgct cagatcctcc
     2041 ccgatcgcgc acagctcacc ggtgcaggtc cgcgagaacg cgaacggcat gaacaccacc
     2101 accacgtgct tcttgccgcg gtagtccgac agcctccacg gctgcccgtg ctggtcccgc
     2161 agctcgaagt ccggcgcctc cgcaccgatc tgcagttcct gctgttcgcc cacctgtgtc
     2221 gcctcctggt cgtccgcccg cgaagccgac agtctagggc catgccaccc agcgccgacg
     2281 ccgccgaccg cggcaccccg cacgctgccg ccgccggctg gcggctgctc ggcgtcgagc
     2341 gcggcgagcc cgcggcgctg ctggcccggg gccttgagct gctggacggc ctgtcccccg
     2401 gcgacccgcc cgtgctgcgc tggtacgtgc cgagcgcgcc cgcgctcgtg ctcggccgcg
     2461 gccagcgcga caccgacgtg cgcacggacc tgcccgtggc gcgccgccac tccggcggcg
     2521 gcgccgtcct catggacgcc gacctgctga gcctcgacgt cgtgtttccc gccggccatc
     2581 cgatggccgc cggcgacctc ggcgaggcgt tcctgcgggt cggcgaggca tgggccgcgg
     2641 cgctgggcga cctcggcgtc accggactgg aggtgcaccg cggcgcctcc cccgccagcc
     2701 gcctgggcgg cccgcgcgag cgcctgctcg ccgcggtctg ctacgcgctg ccggggcgca
     2761 gcgccagcgc cacagcagcc cgcactggac gagcgtgccg tggcggcgcc ggcgctgcgc
     2821 caggccgacc agcttgcgcc cgccgtgcag cacctcgccg cgccccggca gcgcgtagca
     2881 gaccgcggcg agcaggcgct cgcgcgggcc gcccaggcgg cttgcggggg aggcgccgcg
     2941 gtgcacctcc agtccggtga cgccgaggtc gcccagcgcc gc
//
LOCUS       DATMKC010000443         3694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_132894, whole genome shotgun sequence.
ACCESSION   DATMKC010000443 DATMKC010000000
VERSION     DATMKC010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132894"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VNU01_12825"
     CDS             complement(<1..466)
                     /locus_tag="VNU01_12825"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWH33546.1"
                     /translation="MTEPGASSSERGAVLVLHGFTARPRTVEPLGAHLAAAGFTYAIP
                     PLRGHGTHYRDLAAVNWQDWVEDGRNTFESLARHHSRVSIVGHSVGALVAAALAAGDP
                     RVASLVLVAPAILYANPAVTLLPLLRRFVTEWPSGPDSINDPALRTAYAGTNY"
     gene            complement(503..1081)
                     /locus_tag="VNU01_12830"
     CDS             complement(503..1081)
                     /locus_tag="VNU01_12830"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWH33547.1"
                     /translation="MSSSFTPDAWSARLAARQRRALQIAAYRRAAVLVPFTLEDEPRL
                     LLTVRTSHLPTHQGQIAFPGGSIHAGETPRDAALREAHEEVGLEPTQVQVLGDLDDVW
                     TPQGFHVTPVLGAFPTSAVLRHDPGEVAEMLLVPVADLRAIEPRTETRPLRPGTTLPP
                     GLARGHVYHYDWRGHDIWGMTAQIVHDLLSIT"
     gene            complement(1078..1704)
                     /locus_tag="VNU01_12835"
     CDS             complement(1078..1704)
                     /locus_tag="VNU01_12835"
                     /inference="COORDINATES: protein motif:HMM:NF008986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HWH33548.1"
                     /translation="MDRLLEVMRRLRAPDGCPWDREQTHLSLRPYLLEEAAEAVDALG
                     GDDPLEAAGELGDVLLQVAFHAVIAEEEGAYDYGTIEGAIVEKLIRRHPHVFGDVHAP
                     DSDAVVRNWQAIKAEERSGKPEKHPAERVPRSLGALARYREVSKAAGRPKGDREAVLA
                     ALREAAGPEGVAEVLAAVADWARGEGVDAELALRERADAIAREAAARP"
     gene            complement(1729..2667)
                     /locus_tag="VNU01_12840"
     CDS             complement(1729..2667)
                     /locus_tag="VNU01_12840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWH33549.1"
                     /translation="MQLSSDKVKLFAGTANPVLAGAIARTLGLPLGALSVGRFPDGEV
                     KVRLEESVRGDDVYVVQSTCPPVDSHLMELLTLVDALRRSSAGRINAVIPYFGYARQD
                     KQVTGREPITAKLVANLLESAGVDRVITVDLHARQVQGFFDVPVDQLSALRTMSGYLR
                     QTAAPNTVVVSPDTGRGSEARRLADMLGLPLALLYKRRHSDTETEVTAVIGDVAFQRV
                     LLVDDVISTGGTLRRAVEALLENGAVPDIRICATHAVLAGDARQYLSHDAIKEVIVTD
                     TIPLRPGDPYTVLSIAPLIASAIYHVHTHQSVSSLF"
     gene            2807..3451
                     /gene="cmk"
                     /locus_tag="VNU01_12845"
     CDS             2807..3451
                     /gene="cmk"
                     /locus_tag="VNU01_12845"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWH33550.1"
                     /translation="MADGPLIVTIDGPAASGKSSVARGVASSLGVPYLSSGLLYRLVA
                     VAALAGNVDPTSEAALASLLARHDLRLVPDPAGNRVLLDGRDVTDETSSSRVDETVSR
                     VAQHPRVRQWVNESLKRLPGPFVAEGRDMGAAVFPEAPVKLYLTASSRVRAVRRSRER
                     PEDTDAIEAAIVERDARDAVNSRPAPDAVTLDSSELNLEQVIDRALDEVRRRIG"
     gene            3448..>3694
                     /locus_tag="VNU01_12850"
     CDS             3448..>3694
                     /locus_tag="VNU01_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33551.1"
                     /translation="MTLKQPGEAIHENKSLWIYDLVKATTRLYFNLRGFRMIDAHHIP
                     QTGGAILSANHITGMDPFAVGQPSRRRVYFMTKKELFK"
BASE COUNT          549 a         1263 c         1374 g          508 t
ORIGIN      
        1 ggtagttggt tccggcgtag gccgttcgga gggcagggtc gttgatgctg tccgggccgg
       61 acggccactc cgtcacaaag cgcctgagga gcggcaggag ggtcacggcg gggttcgcgt
      121 agaggatggc gggcgcaacc agcacgagcg aggcgacgcg cgggtcgccg gcggcgaggg
      181 cggcagccac cagcgcaccg acggagtggc cgacgatgct cacccgggag tggtgccggg
      241 ccagcgactc gaaggtgttc cggccgtcct ccacccagtc ctgccagttc acggccgcca
      301 ggtcgcggta gtgggtgccg tgcccgcgca ggggggggat cgcgtacgtg aagccggccg
      361 ccgcgaggtg cgcgcctaga ggctcgacgg tcctcggacg ggcggtgaag ccgtgcagga
      421 ccaggacggc cccgcgctcg ctgctggaag ctcccggttc ggtcatggag tgatcgaccc
      481 cgagactaac aggcgcgggc gctcaggtga tcgagagcag gtcgtgcacg atctgggctg
      541 tcatgcccca gatgtcgtgg ccgcgccagt cgtagtgata gacgtgaccg cgcgccaagc
      601 ccggcgggag ggtggtgccc ggccgcagcg gccgcgtctc ggtgcgcggc tcgatggcgc
      661 gcaggtccgc gaccggcacg agcagcatct ccgcgacctc gccagggtcg tggcggagca
      721 ctgccgaggt ggggaaggcg ccgagcaccg gcgtcacgtg gaagccctgc ggcgtccaga
      781 cgtcgtccag gtcgccgagc acctgtacct gggtgggttc gagacccacc tcctcgtgcg
      841 cctcgcgcag ggctgcgtcg cgcggcgtct ctccggcgtg gatggagccg cctgggaagg
      901 cgatctggcc ctgatgcgtg gggaggtggc tggttcggac ggtgagcagc aggcgcggct
      961 cgtcctcgag ggtgaagggc accagcacgg ccgcacggcg gtaggcggcg atctgaaggg
     1021 cgcgacgctg cctcgcggcg aggcgcgcgc tccaggcgtc gggcgtgaag ctgctgctca
     1081 aggccgggcg gcggcctccc gggcgatggc gtcggcacgc tctcgcaggg cgagctccgc
     1141 gtccactccc tcgccgcgcg cccagtccgc gactgccgcg aggacctccg cgaccccctc
     1201 ggggccggcg gcctcccgca gggcggcgag gacggcctcg cggtcgccct tcgggcggcc
     1261 ggctgccttc gagacctcgc ggtagcgagc gagcgcgccg agggagcggg ggacgcgctc
     1321 ggccgggtgc ttctcgggct tgccgctgcg ctcctccgcc ttgatcgcct gccagtttct
     1381 cacgacggcg tcggagtcgg gcgcgtgcac gtccccgaag acgtgcgggt ggcggcggat
     1441 gagcttctcg acgatggcgc cctcgatggt gccgtagtcg taggcgccct cctcctcggc
     1501 gatcaccgcg tggaaggcaa cctgcaggag cacgtcgccg agttccccgg cggcctccag
     1561 ggggtcgtcg cctcctaggg cgtcgacggc ctcggcagcc tcctcgagca ggtaggggcg
     1621 caggctgagg tgcgtctgct cgcggtccca ggggcagccg tccggggcgc gcaggcggcg
     1681 catcacttcg agcaggcggt ccatacggcg cgactctacc ggggcggcct agaagaggct
     1741 gctgaccgac tggtgcgtgt gcacgtggta gatcgcggac gcgatgagcg gcgcgatgga
     1801 gagcaccgtg tagggatcgc ctggacggag ggggatcgtg tcggtcacga tcacctcctt
     1861 gatcgcgtcg tgagacaggt actgccgggc gtcgccggcc agcaccgcgt gcgtggcgca
     1921 gatgcggatg tccggcacgg cgccgttctc cagtagagcc tccacggcgc ggcgcaaggt
     1981 gccgccggtg gagatcacgt cgtccaccag gagcacccgc tggaaggcca cgtcgccgat
     2041 gacggccgtc acctcggtct cggtgtcgga gtggcggcgc ttgtagagca gggccagcgg
     2101 caggccgagc atgtcggcga ggcggcgcgc ctccgagccg cgccccgtgt cgggcgagac
     2161 caccaccgtg ttcggcgcgg ccgtctgccg caggtacccg gacatcgtgc gcagggcgct
     2221 caactggtcc acgggaacgt cgaagaaacc ctgcacctgc cgggcatgca ggtccaccgt
     2281 gatgacgcgg tccacgccgg ccgactccag caggttcgcg accagcttcg ccgtgatcgg
     2341 ctcgcgcccc gtcacctgct tgtcctgccg cgcgtaaccg aagtaaggga tcacggcgtt
     2401 gatgcgcccc gccgaagaac gtcgcagcgc gtccaccagc gtcagcagct ccatgaggtg
     2461 cgagtccacc ggcgggcagg tgctctgcac gacgtacacg tcatcgccac gcaccgactc
     2521 ctcgaggcgc accttgacct cgccgtcagg gaagcggccg acactcaggg cccccagcgg
     2581 caggcccagg gtacgggcga tggcaccggc caggaccggg ttggcggtgc cggcgaagag
     2641 cttcaccttg tcgctggaaa gctgcatcca aacgcgatga taccggtagc gcgcgggggc
     2701 caaacgtcag cagtgtgttc ctgccagagc gtccggagcg ggcagctata ctcgcgggcg
     2761 gcatgaagac gaccacgggc cgccaggctg gagcgcaggg aagttgatgg cggacgggcc
     2821 gctcatagtg accatcgacg ggccggcggc atccggcaag tccagcgtgg cgcgcggcgt
     2881 cgccagcagc ctcggcgtgc cctacctgtc cagcgggctg ctctaccgcc tggtcgcggt
     2941 cgcggccctc gccggcaacg tggaccccac gagcgaggcc gcgctcgcca gccttcttgc
     3001 ccggcacgac ctgaggctcg tgcccgaccc ggcaggcaac cgcgtgctgc tcgacggacg
     3061 cgacgtgacc gacgagacct cgtccagccg cgtggacgag acggtctccc gcgtggcgca
     3121 gcacccgcgc gtgcggcagt gggtgaacga gagcctcaag cgcctccccg gaccgttcgt
     3181 ggccgaggga cgcgacatgg gagcggcggt cttccccgag gcgcccgtca agctctacct
     3241 gacggcctcc agccgcgtcc gggccgtgcg gcgctcgcgg gagcgcccgg aggacacgga
     3301 cgccatcgag gcggccatag tggagcggga cgcgcgtgac gccgtgaact cccgcccggc
     3361 gcccgacgcc gttacactcg actcctcgga actcaacctg gagcaggtga tagaccgcgc
     3421 gctggacgag gtgaggaggc gaatcggttg acactgaagc agcctggcga ggcgatccac
     3481 gagaacaaga gcctctggat ctacgacctg gtgaaggcga cgacccgcct ctacttcaac
     3541 ctgcgcggct tccgcatgat cgacgcgcac cacatcccgc agaccggggg cgccatcctc
     3601 tccgcgaacc acatcacggg aatggacccc ttcgcggtgg gccagccctc caggcgccgc
     3661 gtctacttca tgactaagaa ggagctcttc aagt
//
LOCUS       DATMKC010000444        15418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_54996, whole genome shotgun sequence.
ACCESSION   DATMKC010000444 DATMKC010000000
VERSION     DATMKC010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 15418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15418
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54996"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1445
                     /locus_tag="VNU01_12855"
     CDS             180..1445
                     /locus_tag="VNU01_12855"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWH33552.1"
                     /translation="MQRRTRWAAVLLLFVALVAVAPAWAQERPFGDTQVFARVGQPGQ
                     PEGIAVDSRGRVYVSTNNRGKGGPGPSKIFRYSPAGRLEREYVAEGQNLAGEHGLLGI
                     AIDAADRLYVMDYDPARVLRFHPETGAQETYATIPNLPACGPGAAPDGSCEPGAQDTT
                     PWPNWPVFDADGNLFLTDLHQATIWKIAAGGGVAQPWHQSADYESVFSLNGIQFDRRD
                     GSLVYVLTGGFNADSPARGVIHRLAVNPDGTPGERITVFQTLPGEGPDGLALGESGRI
                     YVALVTSSHILVLEPDGTEVARVPDPVSNRTQQIPYDAPASVAFRGTSILVTNHSYFT
                     ANPESWAVLDMAVGEVGMALHHPAIGGRGAPQAPPATPSTPVPRPAGELPASGPGPVM
                     PVAGLVALAMGVSSKGLTRTPRTHRRPVS"
     gene            complement(1707..2483)
                     /locus_tag="VNU01_12860"
     CDS             complement(1707..2483)
                     /locus_tag="VNU01_12860"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWH33553.1"
                     /translation="MSVRVERDGPVVLVTIDRPEVRNAVDGPTAHLLAEAFGDFDRDE
                     AAAVAVLTGAGGTFCAGADLKALASGGRVNPLLPPDGPESVGPMGPSRLLLRKPVIAA
                     VEGNAVAGGLELALWCDLRVAAQDAVFGVYCRRWGVPLVDGGTVRLPRLVGHSRAMDM
                     ILTGRGVSGQEALDFGLANRLVPTGQACEAAMELARQLAAFPQVCLRSDRLSAYQQWS
                     LELPEALAQEFDGGMAVLASGEAIEGAGRFASGEGRHGAF"
     gene            2616..3638
                     /locus_tag="VNU01_12865"
     CDS             2616..3638
                     /locus_tag="VNU01_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWH33554.1"
                     /translation="MRLGINIGYSGSAIGGVWPMIEQADKLGYDSVWAAEAYGSDAVT
                     VLSYIAAKTERIKVGSAILQMPARTPANTAMTAMTLDALSGGRVLLGLGLSGPQVVEG
                     WHGVPYGKPLRRTREYVEIVRAAIAREAPLEYDGQEYQIPYRGEDATGLGKPLKSILH
                     PVRDRIPIYLASIGPKNVELTAEIADGWLPIFYSPEREGIYNEHLDAGLAKAGRKADE
                     IDIAPTAYVAMGDDVNACRDMLRPMFALYVGGMGARGRNFYFDLACRYGFEEAATKVQ
                     DLYLDGKKAEAAAAVPDELVDECALVGPVERIVDRLAAWKESRVGTLIVGTMQPEVLE
                     PLINAL"
     gene            complement(3693..4574)
                     /locus_tag="VNU01_12870"
     CDS             complement(3693..4574)
                     /locus_tag="VNU01_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWH33555.1"
                     /translation="MWTDDRVPRFWQRLGLPGIVDIHTHFMPQPVLDKVWGWFDSVRG
                     ADGRQLWPVLYREREEDRIALLRALGVRRFTSLFYAHKPGMARWLNDWGAQFAARVPE
                     CKQSATFYPEEGVDAYVAEAIEGGARVFKIHLEVGRFDARDPLLRPVWRRLEREGAVV
                     VVHAGSGPVPGPFTGPEFFGPVLEAAPELAAVIAHMGGPEYREFLDMALRYPNVRLDT
                     TMTFTDFMQQMGSVYPPDCLERIAEHPERVLLGTDFPNIPHPYAHQLQALERLGLGDD
                     WLRAACHDNAAALLGLP"
     gene            complement(4580..6160)
                     /locus_tag="VNU01_12875"
     CDS             complement(4580..6160)
                     /locus_tag="VNU01_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWH33556.1"
                     /translation="MDRYAAQRRRERVVAAARWFAVGFLALHVVLAEPPGASGGVDGP
                     TRAAAWLVVGLYALVCVAAEVGIRVTNRREGFDRLGMALLLADVTLASAFVWLYRSDP
                     SSTTWAVLTLLPLEGAVRFGMAGALAAWVGTLPALILPALSPEGAEVALADVVLPPLF
                     LFVLALIGGVVGRDLERQVVVLARVNRAARRIASRRDPEEVLATLCDETVRCLDARSA
                     TVVAERDGARRVLWASPWREADETDHLGELVIPLPWATEDEPMWFMVHTGRGTAALAP
                     QVARALVESAAIAIGGARLIAIEQRTNARLRYLEALRKRFVATIAHDLRLPLTVFKGV
                     AHVLRARRDEIEPQEVDDLLASVERQANRLGRLADDLLDAARADAGRITIVREPCRLR
                     AVVEQAAADFAEPVLIDIDPELELQADRGRIDRLVWNLLGNAQKYGHPPYEVRAEAGR
                     ELVELSVRDHGPGLPPEVRERLFEEFAAGDSEGVGLGLAIVRQLAEAHGGDVRYSDAD
                     PGARFTVTLPRHPDAGAE"
     gene            6219..6362
                     /locus_tag="VNU01_12880"
     CDS             6219..6362
                     /locus_tag="VNU01_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33557.1"
                     /translation="MPRVQPAPDLAERLAPLEVTALDGSAVPLGTLWDQRPLLLVHLR
                     HFG"
     gene            6363..6767
                     /locus_tag="VNU01_12885"
     CDS             6363..6767
                     /locus_tag="VNU01_12885"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HWH33558.1"
                     /translation="MFCREHVAQLRDHKAEIDRRDVAVAAVGTGDLMYARDFAEDKRV
                     NFPLYVDDAKASYKAVGTRKGSARSFAKPRVIASAARVTAKGHIQGKLGPAHMVLGAT
                     HVIRPDGSVAFAWVNGDFDDNADFAEALRSLD"
     gene            complement(6777..8045)
                     /locus_tag="VNU01_12890"
     CDS             complement(6777..8045)
                     /locus_tag="VNU01_12890"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HWH33559.1"
                     /translation="MRGASAGPAQEYLPRVVDEQLARLVASLPAVAVEGPKAVGKTET
                     ARRVAAGEVLLDRPAARALAEADPDQALSGPAPLLLDEWQLVPPVWDAVRRAVDVDPS
                     PGRFVLTGSARPQHVTAHTGAGRVVSLRMRPMTLPERRQSPTTVSLAALLSGARGAVT
                     GEATMDLAGYASEIVASGFPGLRALGEGARTVALDGYVDQIIQRDVPESGYRLRRPET
                     LRRWLRAFAAATGTTASLEAIRDAATAGDGSTPAKVTVLAYHGALERLWVVDDLPGWA
                     PSLRQLTKVGQAPARHLADPALAAALLGVDTAALLSDGVLLGRMFESLAALSVRVFAQ
                     AAGARVWHLRTHGGGHEIDLIVERRDGAVVAIEVKLGSAVDDRDVAHLRWLASRIGER
                     LVDIAVVHSGPHAYRRRDGVAVIPLELLGP"
     gene            8223..9899
                     /gene="ilvD"
                     /locus_tag="VNU01_12895"
     CDS             8223..9899
                     /gene="ilvD"
                     /locus_tag="VNU01_12895"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWH33560.1"
                     /translation="MSINRPRSTDVTEGFQRAPARAMLRAVGMSEEDFSKPQIGVASS
                     WNEVTPCNLPLARLARVAKEGVRAAGGFPVEFVTIAVSDGISMGHEGMRASLVSREVI
                     ADSVETVMHAERFDALVAFAGCDKSLPGMIMAAARLDLPAVFLYGGTIMPGEVDGRKI
                     DIKDVFEAVGAHAAGTMSDEELHTIESNACPGEGSCGGMYTANTMACASEALGIALPG
                     AASPPAIDPRREDLARRSGQAVLGLLERGINARKIITKESLENAIAVVMAVGGSTNAV
                     LHLLAIANEARVELSLADFDRIGRRVPHIVDSKPHGRFLMSEIDEAGGLPVVMKALLE
                     RDLLHGDCLTVTGRTIAENLAGMDIAAPDGEVVRPLDRPINAEGGLAVLHGSLAPNGA
                     IVKVAGLDDMVFEGTARVFDGEQAAMDYTLANKLVPGDVIVIRYEGPKGGPGMREMLA
                     VTAAVKGVGRGGDVALLTDGRFSGATHGLCIGHVAPEAVDGGPIALVADGDRIRIDVP
                     NRTLDVLVDEAELERRRADLKHPEPRYTSGVLAKYAKLVSGAELGAITNP"
     gene            10011..12152
                     /locus_tag="VNU01_12900"
     CDS             10011..12152
                     /locus_tag="VNU01_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWH33561.1"
                     /translation="MKRVRLLLLVLLACALVAPASASASAEHSSSAAAQVAATTAGQA
                     EQEDGDPAGERLSVVVRTEHGLDVRRLPAGAPGEAEEALRRLRALDSVVAAERDVPVK
                     ALGDPLRQHQWGLDRLHAEQAWTSTQASGQKVAVVDTGVDAAHPDLAGVVLSGYDAFT
                     PGGDGRNDANGHGTHVAGIVAAVSGNGIGVEGLARSAKILPVKVLQDDGSGYSSDVAE
                     GILWAIANGATVVNLSLGSPNTSSSVDAAIEQAIQKGVVVVAASGNEGAKGNPVMWPA
                     ALPEVVSVGAIDSTDQRASFSGHGDWLDLVAPGVQIASTMSGEYMYSSGTSMAAPFVS
                     ATAALVRTRYPSLTPPQLADHLLATAEDLGPAGEDPEYGVGLVDTVKALTTAPPGLDL
                     QAKDAVTPVAATSDLVRGATEVSRTAFPATAAATVAVLARDDAFADSLAGSALASTDG
                     PILYTTGGPDAPLRAETATELTRVLAPGGVVHVLGGPGAVSERAVADVQALGFTVVRL
                     QGPSRVETALAIADAVSPAPSRVMLARADQWADAVTGGAYAASSGVPLLLTQSDSLHP
                     AVAAWLAAKRPSEVILLGGESALSANVAAAVPGRATRVSGPTRAGTAVAVAKSLWGRR
                     DGAPGDRFVAVDGFASDSWSPALAASVLSARQAAPQILVNGGAPSTVSPETEAYLTEL
                     GYSSSKSATAYLVGPKVGAGTEDSLNALLTQ"
     gene            complement(12180..13265)
                     /locus_tag="VNU01_12905"
     CDS             complement(12180..13265)
                     /locus_tag="VNU01_12905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWH33562.1"
                     /translation="MAVEPGSTPEDLEVLARASLHAGAYDYYVSGADDERTLRENLAA
                     WERLRLRPRVLRDVSAVSTEAHVLGVPVAAPVLVAPTAYQRLAHPDGEAATASGAAAF
                     GTVMVVSTFANTAMEEVAAAAPDAPRWFQLYVHTDRGLTAELVRRAAASGYRAIVLTV
                     DLPVLGYRRRDERNNFALPMGVVKANLGDTPSAADVPTDGSALRAYAEHAVESSLTFD
                     DLGWLRGLSDLPLVVKGVLRGDDAAACVAAGADAIVVSNHGGRQLDGAIPTAEALPDV
                     VRAVGERAEVYVDGGIRRGTDVVRALALGARAVLLGRPVLWGLAVDGADGVRAVLENY
                     RVELARAMALCGTPAVTDVTADLLAER"
     gene            13297..15216
                     /locus_tag="VNU01_12910"
     CDS             13297..15216
                     /locus_tag="VNU01_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWH33563.1"
                     /translation="MRQRRGTRGRLVTALVALVAGAALLPLLPATAAPNGYVEMSDGV
                     LIAVNVRPPRNFVEGRRYPAVFEMSGYDGGSASDQTLMGDIADAFGYEGEVPLREDSR
                     QLTHRFEGDYFTIHASVRGTGCSGGTFDLFSWRAALDGHEVIEWIARQPWSDGNVALM
                     GHSYGGITGFMIAATRPPHLRAATLSGLIDDLYRGITYPGGVSNYGFPLAWTGVIRPF
                     YDLGGGLAPGIVRTQDPVCAQNQASKNRTVLEDPIVNGVGDTDSNWWRSRSLISYVHL
                     IDVPMHITGAYQDEQTGPRGPFHLFEKLPTHIPRRLVMTNGDHGTQQGPDAVWDDRVA
                     WVDQFTRGLPNRFDPIPAPGGGTRAHSSTRVLLELHSGVHEGSQGTHDERHPNGVVDK
                     TAFPLENTQWTDLYLRGGGALSTTPPAEAEEPARYVSGSGRQSWSYQAGPAAGSELST
                     PDGPDEAVYATPAFTQDVLIAGPMAATLFVDTTAPDTELFVQLIDEGPDGSRSYLQRG
                     MLRASHRALNVAMSDKTADGRIYRPFHPHTNPTPVVPGQVNEYVVEIFPVGHVLRAGH
                     RLVVKVHAPPRVDSFNAYVPRYAPSVNTIHHGPRTPSRLLLPVVPLAGVRLGPEIACG
                     RQEAVRCVPGGADGS"
BASE COUNT         2148 a         5857 c         5503 g         1910 t
ORIGIN      
        1 ggacccgcca cgcaaattcc gccgcccccc gtacccgccg ccgccgccgg cggcgggtac
       61 ggggggcggc ggaatttgcg tggcgggtcc tccccttccg gcccggcctg ggctacggtg
      121 cggccactgg cgggccgagc ccgccggcgg caggctcgac tgggcgagga ggggccgagg
      181 tgcagcgccg gacgcggtgg gcggcggtcc tgctgctgtt cgtcgccctg gtcgcggtcg
      241 cgccggcctg ggcgcaggaa cggcccttcg gcgacacgca ggtgttcgcg cgcgtcggcc
      301 agccggggca gcccgagggc atcgcggtcg actcgcgcgg ccgcgtctac gtcagcacca
      361 acaaccgcgg caagggcggc cccggaccct ccaagatctt ccgctactcc cccgcggggc
      421 ggctggagcg cgagtacgtc gcggaggggc agaacctcgc cggcgagcac ggcctgctgg
      481 gcatcgcgat cgacgcggcc gaccgcctct acgtcatgga ctacgacccg gcgcgcgtcc
      541 tgcgcttcca ccccgagacc ggcgcgcagg agacctacgc caccatcccg aacctgcccg
      601 cgtgcgggcc gggcgccgcg ccggacggct cctgcgagcc cggcgcgcag gacaccaccc
      661 cgtggccgaa ctggccggtg ttcgacgcgg acggcaacct cttcctcacc gacctgcacc
      721 aggcgaccat ctggaagatc gcagccggcg gcggcgtcgc ccagccgtgg caccagtccg
      781 ccgactacga gtcggtcttc agcctcaacg gcatccagtt cgaccggcgc gacggcagcc
      841 tggtgtacgt gctcaccggc ggcttcaacg ccgactcacc ggcgcgcggc gtgatccacc
      901 gcctggcggt caacccggac ggcacgccgg gcgagcgcat cacggtgttc cagaccctgc
      961 ccggcgaggg gcccgacggc ctcgcgctgg gcgagtccgg ccgcatctac gtcgccctgg
     1021 tgacctccag ccacatcctg gtactggagc cggacggcac cgaggtcgcg cgggtgccgg
     1081 accccgtcag caaccgcacc cagcagatcc cctatgacgc gcccgcctcc gtcgccttcc
     1141 gcggcacgtc catcctggtg accaaccact cctacttcac cgccaacccg gagtcctggg
     1201 ccgtgctcga catggcggtc ggcgaggtcg gcatggcgct gcaccacccc gccatcggcg
     1261 gccgcggcgc gccgcaggcg ccgccggcca ccccctcgac gccggtgccg cgcccggccg
     1321 gcgaactgcc cgccagcggg cccggcccgg tcatgcccgt ggctggcctg gtcgcgctgg
     1381 cgatgggcgt gagcagcaag ggactcacac gcactccgcg cacgcaccgt cgcccggtgt
     1441 cctgaggacc ccgggcgacg ggtgtcgtcg gtagcggccg ggatggctga ggaggccgga
     1501 gagaaccctg gccgtcaccg acaacgtctg tgtggcgggc agccgagaca gcccgtccga
     1561 ggaaggctgg atctccattt cgcgggagtt gcacaccccc gaaatcgtga agatcggccc
     1621 gcccattgcg atcttcggat ctctcctgtc cgagaccttc aaagatcggc aggtcggggc
     1681 cccgacctga gcgggagttg tccggctcag aacgcgccgt ggcgaccctc gccggaggcg
     1741 aaccgccccg cgccctcgat cgcctcgccg gaggccagca ccgccatgcc gccgtcgaac
     1801 tcctgcgcca gcgcctccgg cagctccagc gaccactgct ggtaggcgga cagccggtcg
     1861 ctgcgcaggc agacctgcgg gaacgcggcg agctggcgcg ccagctccat ggccgcctcg
     1921 cacgcctgac ccgtgggcac caggcggttg gccaggccga agtccagcgc ctcctggccg
     1981 gagacgccgc ggccggtgag gatcatgtcc atggcgcggc tgtggccgac cagcctgggc
     2041 aggcgcacgg tgccgccgtc gaccagcggc acgccccagc gccggcagta cacgccgaag
     2101 acggcgtcct gcgccgccac gcgcaggtcg caccacagcg ccagctccag cccgccggcc
     2161 accgcgttgc cctccacggc ggcgatcacc ggcttgcgca gcagcaggcg cgacggcccc
     2221 atggggccga ccgactcggg gccgtccggg ggcagcagcg ggttgacgcg ccctcccgat
     2281 gccagcgcct tgaggtccgc gcccgcgcag aacgtgccgc cggcgccggt gaggaccgcg
     2341 acggcggccg cctcgtcccg gtcgaagtcg ccgaaggcct cggccagcag gtgcgccgtg
     2401 gggccgtcga cggcgttgcg gacctccggc cggtcgatgg tgaccagcac caccggcccg
     2461 tctcgctcca cacggacgct catcggcacc tcacccccgt cacgcttgcg gattcgctgc
     2521 ggaccctagc ggcagccggc gccgggcggc aacggcgtcc ggaggtctag gatcggcgcg
     2581 cgcgaatccg cgccatggca ccgaagggag cacccatgag gctcggcatc aacatcggct
     2641 actcggggtc tgcgatcggc ggcgtgtggc cgatgatcga gcaggccgac aagctcggct
     2701 acgactcggt gtgggcggcc gaggcgtacg gctccgacgc ggtgacggtg ctgagctaca
     2761 tcgccgccaa gaccgagcgc atcaaggtgg gcagcgccat cctgcagatg ccggcgcgca
     2821 cgccggcgaa caccgcgatg accgcgatga cgctggatgc gctgtccggc ggacgggtgc
     2881 tgctggggct ggggctgtcc ggcccgcagg tggtggaggg ctggcacggg gtgccctacg
     2941 gcaagccgct gcggcgcacc cgcgaatacg tggagatcgt gcgcgccgcg atcgcgcgcg
     3001 aggcgccact ggagtacgac ggccaggagt accagatccc ctaccgcggc gaggacgcca
     3061 cggggctcgg caagccgctc aagtcgatcc tgcacccggt gcgcgaccgc atcccgatct
     3121 acctggcgtc catcgggccg aagaacgtgg agctcaccgc ggagatcgcc gacggctggc
     3181 tgccgatctt ctactcgccg gagcgcgagg gcatctacaa cgagcacctc gacgccgggc
     3241 tggccaaggc gggccgcaag gccgacgaga tcgacatcgc gccgaccgcc tacgtcgcga
     3301 tgggcgacga cgtgaacgcg tgccgcgaca tgctgcggcc gatgttcgcg ctgtacgtgg
     3361 gcggcatggg cgcgcgcggc aggaatttct acttcgacct ggcgtgccgc tacggcttcg
     3421 aggaggccgc caccaaggtg caggacctgt acctggacgg caagaaggcg gaggccgccg
     3481 cggccgtgcc cgacgagctg gtggacgagt gcgccctggt cggcccggtc gagcgcatcg
     3541 tcgaccgcct ggccgcgtgg aaggagtccc gcgtcggcac gctgatcgtc ggcacgatgc
     3601 agccggaggt cctggagccc ctgatcaacg cgctgtagtc cccctgcgga gtcgattccg
     3661 gccgatagcg gccacgatcg cctccgcagg cgtcagggca ggccgaggag cgcggcggcg
     3721 ttgtcgtggc aggcggcgcg cagccagtcg tcgccgaggc ccaggcgctc cagcgcctgg
     3781 agctggtggg cgtacgggtg cgggatgttc gggaagtcgg tgcccagcag cacgcgctcc
     3841 gggtgctcgg cgatgcgctc caggcagtcc ggcgggtaca cgctgcccat ctgctgcatg
     3901 aagtcggtga acgtcatggt ggtgtccagg cgcacgttcg ggtagcgcag cgccatgtcc
     3961 aggaactccc ggtactccgg cccgcccatg tgggcgatca ccgcggccag ctccggcgcg
     4021 gcctccagga ccggcccgaa gaactccggc cccgtgaacg ggccgggcac cggcccgctc
     4081 ccggcgtgca ccacgaccac cgcgccctcg cgctccaggc gccgccacac gggccgcagc
     4141 agcgggtcgc gggcgtcgaa gcgcccgact tccaggtgga tcttgaacac gcgcgccccg
     4201 ccctcgatcg cctcggcgac gtaggcgtcc acgccctcct cgggatagaa cgtcgccgac
     4261 tgcttgcact ccggcacgcg cgccgcgaac tgcgcgcccc agtcgttcag ccaccgcgcc
     4321 atgccgggct tgtgcgcgta gaacaggctg gtgaagcggc gcacgcccag cgcgcgcagc
     4381 agcgcgatcc ggtcctcctc gcgctcccgg tacagcaccg gccacagctg cctgccgtcc
     4441 gcgccccgca cggagtcgaa ccagccccac accttgtcca gcaccggctg cggcatgaag
     4501 tgcgtgtgga tgtcgacgat ccccggcagg ccgagtcgct gccagaaccg cggcacgcgg
     4561 tcgtcggtcc acatggcgct cactccgcgc cggcgtccgg gtgccgcggc agcgtcacgg
     4621 tgaagcgcgc gccgggatcg gcgtcggagt agcgcacgtc gccgccgtgc gcctcggcga
     4681 gctggcgcac gatcgccagg cccaggccga caccctcgga gtccccggcc gcgaactcct
     4741 cgaacagccg ctcgcgcacc tccggcggca ggccggggcc gtggtcgcgc acgctgagct
     4801 ccaccagctc cctgccggcc tccgcgcgca cctcgtaggg cgggtgcccg tacttctgcg
     4861 cgttgcccag caggttccac accaggcggt cgatgcggcc gcggtcggcc tggagctcca
     4921 gctccggatc gatgtcgatc agcaccggct cggcgaagtc ggccgcggcc tgctccacca
     4981 cggcgcgcag gcggcacggc tccctgacga tggtgatgcg cccggcgtcg gcgcgggccg
     5041 cgtccagcag gtcgtcggcc aggcgcccca ggcggttggc ctgccgctcc acgctggcca
     5101 gcaggtcgtc cacctcctgc ggctcgatct cgtcgcggcg cgcgcgcagg acgtgcgcca
     5161 cgcccttgaa gacggtgagc ggcaggcgca ggtcatgggc gatggtcgcg acgaagcgct
     5221 tgcgcagcgc ctccaggtag cgcagccgcg cgttggtgcg ctgctcgatg gcgatgagcc
     5281 tggcgccacc gatggcgatg gcggccgact ccaccagcgc gcgggcgacc tgcggcgcga
     5341 gcgccgcggt gccccggccg gtgtgcacca tgaaccacat cggctcgtcc tcggtagccc
     5401 acggcagcgg gatcaccagc tcgcccaggt ggtcggtctc gtcggcctcg cgccacggcg
     5461 acgcccacag cacccgccgg gcgccgtcgc gctcggcgac caccgtcgcg ctgcgcgcgt
     5521 ccaggcagcg cacggtctcg tcgcacagcg tcgccagcac ctcctcgggg tcgcgccggg
     5581 acgcgatgcg gcgggcggcg cggttgaccc gcgcgagcac gaccacctgg cgctccaggt
     5641 ctcggcccac gacgccgccg atgagcgcca gcacgaacag gaacagcggc ggcagcacga
     5701 cgtcggccag cgccacctcg gcgccctccg gcgacagcgc cggcaggatc agcgcgggca
     5761 gggtgccgac ccacgcggcc agggcgccgg ccatgccgaa gcgcaccgcg ccctccagcg
     5821 gcagcagcgt gagcacggcc caggtggtgc tggaggggtc ggagcggtac agccacacga
     5881 acgccgaggc cagcgtcacg tcggcgagca gcagggccat gcccaggcgg tcgaagccct
     5941 cgcggcggtt ggtgacccgg atgccgactt ccgcggccac gcacaccagc gcgtacaggc
     6001 cgaccaccag ccaggcagcc gcgcgcgtgg ggccgtccac gccgccgctc gccccgggcg
     6061 gctcggccag gaccacgtgc agcgcaagga agccgaccgc gaaccaacgc gcggcggcga
     6121 cgacccgctc acggcgccgc tgcgcggcgt accgatccat ggcggaaggc taacgggcgg
     6181 tggtgcccgg tgccgtcagg agcgggcatg atgtgcgcat gccacgagtg cagcccgccc
     6241 cggacctcgc cgagcgcctc gcgccgctgg aggtcaccgc cttggacggc tccgccgtgc
     6301 cgctgggcac gctgtgggac cagcgcccgt tgctgctggt ccacctgcgt cacttcgggt
     6361 gaatgttctg ccgggagcac gtcgcgcagt tgcgtgacca caaggcagag atcgaccgcc
     6421 gcgacgtcgc ggtggcggcc gtgggcaccg gcgacctgat gtacgcgagg gacttcgccg
     6481 aggacaagcg ggtgaacttc ccgctgtacg tggacgacgc caaggcgagc tacaaggccg
     6541 tcggcacgcg caagggcagc gcgcgcagct tcgccaagcc ccgcgtgatc gccagtgcgg
     6601 cgcgcgtcac cgccaagggg cacatccagg gcaagctcgg cccggcgcac atggtcctgg
     6661 gcgcgacgca cgtgatccgc ccggacggca gcgtggcgtt cgcgtgggtc aacggggact
     6721 tcgacgacaa cgcggacttc gccgaagcgc ttcgctcgct ggactgacca ggccggtcac
     6781 gggcccagca gttcgagagg gatcaccgcg acgccgtctc gccgacggta ggcgtgcggc
     6841 ccgctgtgca ctaccgcgat gtccaccagg cgctcgccga tccggctggc caaccagcgc
     6901 aggtgcgcga catcgcggtc gtccacggca gagccgagct tcacctcgat cgcgaccaca
     6961 gccccgtccc ggcgctccac gatcaagtcg atttcgtgtc cgccgccgtg agtgcgcaag
     7021 tgccacaccc gggcccccgc cgcctgggcg aacacccgta ccgacagggc cgccagggat
     7081 tcgaacatcc ggccgagcag cacgccgtcg gagagcagag ctgccgtgtc cacgccgagc
     7141 agggcggccg ccagcgccgg gtcggccaag tgccgggcgg gagcctggcc gaccttggtc
     7201 agctggcgca gcgacggagc ccagccaggg aggtcgtcga ccacccacag gcgctccagc
     7261 gcgccgtggt acgccaagac ggtgaccttg gccggcgtgc tgccatctcc ggccgtcgcg
     7321 gcgtcgcgga tggcctccag ggacgccgtg gtccccgtcg cagccgcgaa ggcgcgcagc
     7381 caccgccgca gcgtctccgg ccgccgcagc cggtagccgc tttccggcac gtcgcgctgg
     7441 atgatctgat cgacgtagcc gtccagcgcc accgtccgcg cgccctcccc gagcgcacgc
     7501 aggccaggaa agcccgaggc gacgatctcg ctcgcgtagc cggcgaggtc catcgtcgcc
     7561 tcacctgtca ccgcaccgcg cgcgcccgac aggagcgcgg ccagcgacac cgtcgtgggg
     7621 gactgccggc gctccggcaa ggtcatcggc cgcatacgca gagaaaccac gcggccggca
     7681 ccggtatgcg ccgtgacgtg ctggggccgc gccgacccgg tcaggacgaa ccgccccggg
     7741 ctggggtcga cgtcgacggc gcggcggacg gcgtcccaga cgggagggac cagctgccac
     7801 tcgtccaaga gcagcggtgc cggccccgac agcgcctgat cggggtcggc ctccgccagc
     7861 gcccgcgcgg cgggacggtc cagcagcacc tcgcccgcgg cgacgcggcg cgcggtctcc
     7921 gtcttgccga ccgccttggg gccctcgacg gccacggcgg gcagcgacgc gaccaggcgc
     7981 gcgagctgtt catcgacgac gcgcggcaga tattcctgcg ccggcccggc agacgctccg
     8041 cgcatgggcc ctcctcgctt accatactgt ttctcctgcc tttactctac catttctcga
     8101 cgtttggcca cctcacttct ccacaagggt gggcgcacgc cggcgccggc atgcgcgcct
     8161 cgggcccagg ggtgttcaat gcacaggctt ggtccaggcc ttctccgccc aggagcgcac
     8221 ccatgagcat caaccggccg cgcagcaccg acgtcaccga ggggttccag cgggcgcccg
     8281 cgcgcgccat gctgcgcgcc gtcggcatga gcgaggagga cttctccaag ccgcagatcg
     8341 gcgtggcgtc gtcctggaac gaggtcacgc cgtgcaacct gccgctggcg cgcctggcgc
     8401 gcgtggccaa agagggcgtc cgcgccgccg gcgggttccc ggtggagttc gtgaccatcg
     8461 ccgtcagcga cggcatctcc atgggccacg agggcatgcg ggcctcgctg gtgtcccgcg
     8521 aggtcatcgc cgactccgtc gagaccgtca tgcacgccga gcgcttcgac gcgctggtgg
     8581 ccttcgccgg ctgtgacaag tccctgcccg ggatgatcat ggccgccgcg cgcctggacc
     8641 tgcccgcggt gttcctctac ggcggcacga tcatgccggg cgaggtcgac ggccggaaga
     8701 tcgacatcaa ggacgtcttc gaggccgtcg gcgcgcacgc cgccggcacg atgagcgacg
     8761 aggagctgca caccatcgag tccaacgcct gcccgggcga gggctcctgc ggcggcatgt
     8821 acaccgccaa cacgatggcg tgcgcctccg aggcgctggg catcgcgctg ccgggcgccg
     8881 cctcgccgcc ggccatcgac ccgcgccgcg aggacctggc gcgccgctcc ggccaggccg
     8941 tcctgggcct gctggaacgc ggcatcaacg cccgcaagat catcaccaag gagtccctgg
     9001 agaacgcgat cgccgtggtc atggccgtgg gcggctccac caacgccgtg ctgcacctgc
     9061 tcgccatcgc caacgaggcg cgcgtggagc tcagcctggc ggacttcgac cgcatcggca
     9121 gacgggtccc gcacatcgtg gactccaagc cgcacggccg cttcctgatg agcgagatcg
     9181 acgaggccgg cggcctgccg gtggtgatga aggccctgct ggagcgcgac ctgctccatg
     9241 gcgactgcct caccgtcacc ggcaggacca tcgcggagaa cctcgccggc atggacatcg
     9301 ccgcgcccga cggcgaggtg gtgcggccgc tggaccgccc gatcaacgcc gagggcggcc
     9361 tggcggtgct gcacgggtcg ctggcgccca acggcgccat cgtgaaggtc gccggcctgg
     9421 acgacatggt cttcgagggc accgcgcgcg tgttcgacgg cgagcaggcc gcgatggact
     9481 acacgctggc gaacaagctg gtgccgggcg acgtcatcgt catccgctac gagggcccca
     9541 agggcgggcc cggcatgcgc gagatgctcg ccgtgaccgc ggcggtgaag ggcgtcggcc
     9601 gcggcggcga cgtggcgctg ctgaccgacg gccgcttctc cggcgccacc cacgggctgt
     9661 gcatcggcca cgtcgcgccg gaggcggtgg acggcgggcc gatcgcgctg gtcgccgacg
     9721 gcgaccgcat ccgcatcgac gtgcccaacc gcacgctgga cgtgctcgtg gacgaggcgg
     9781 agctggagcg ccgccgcgcg gacctgaagc acccggagcc gcgctacacc tccggcgtgc
     9841 tggccaagta cgccaagctc gtgtcgggcg cggaactggg cgcgatcacc aacccgtaag
     9901 cgggctgtcg ggccgccgta gcccgggctg actactcctg gcgtcaccca ttggggtgat
     9961 ttgcgactag gcgcctctag ccctgttcgg ggactgccga tccgccaggc atgaagcgtg
    10021 tccggctcct gctcctcgtc ctgctcgcct gcgccctggt ggcccccgcc agcgcgtccg
    10081 cgtcggcgga gcactcgtcc tcggcggccg cccaggtcgc cgcgaccacg gcggggcagg
    10141 ccgagcagga agacggcgac ccggccggcg aacgcctgtc cgtggtggtg cggaccgagc
    10201 acggcctgga cgtgcggcgc ctgcccgccg gagcgcccgg cgaggccgag gaggcgctgc
    10261 gccgcctgcg cgcgctggac tccgtggtcg ccgcagagcg cgacgtgccg gtcaaggcgc
    10321 tgggcgaccc gctgcgccag caccagtggg gccttgaccg cctccacgcc gagcaggcgt
    10381 ggacgtcgac gcaagcctcc ggccagaagg tcgccgtggt ggacaccggc gtggacgccg
    10441 cccaccccga cctggccggc gtcgtgctca gcggctacga cgcgttcacg ccgggcggtg
    10501 acggccgcaa cgacgccaac ggccacggca cccacgtcgc cggcatcgtc gccgcggtca
    10561 gtggcaacgg catcggcgtc gagggcctcg cccgcagcgc caagatcctg ccggtgaagg
    10621 tgctccagga cgacggcagc ggctactcca gcgacgtggc cgagggcatc ctgtgggcca
    10681 tcgccaacgg cgccaccgtc gtgaacctga gcctgggctc gccgaacacg tcctcgtccg
    10741 tggacgccgc catcgagcag gcgatccaga agggcgtcgt cgtggtcgcc gcgtccggca
    10801 acgagggcgc caagggcaac ccggtcatgt ggcccgccgc cctgccggag gtcgtctccg
    10861 tcggcgcgat cgactccacc gaccagcgcg cctccttcag cggacacggc gactggctgg
    10921 acctggtcgc ccccggcgtg cagatcgcct cgaccatgag cggcgagtac atgtacagct
    10981 ccggcacctc gatggcggcg ccgttcgtgt ccgccaccgc cgcgctggtc cgcacgcgct
    11041 acccgtcgct cacgcccccg cagctggccg accacctgct ggccacggcc gaggacctgg
    11101 gcccggccgg cgaggatccc gagtacggcg tcgggctggt cgacacggtg aaggcgctga
    11161 ccacggcgcc gcccgggctg gacctgcagg ccaaggacgc ggtgaccccg gtggcggcca
    11221 ccagcgacct ggtgcgcggc gccacggagg tgagccggac ggcgttcccg gcgacggcgg
    11281 cggcgacggt cgcggtcctg gcgcgcgacg acgcgttcgc agactcgctg gccggttccg
    11341 cgctggcgtc caccgacggc cccatcctgt acacgaccgg cggcccggac gcacccctgc
    11401 gcgcagagac cgccacggag ctcacgcgcg tgctcgctcc cggcggcgtg gtgcacgtgc
    11461 tgggcggtcc gggcgcggtg tccgagcgcg ccgtggccga cgtgcaggca ctgggcttca
    11521 cggtggtgcg cctccaaggt cccagccgtg tcgagacggc cctcgcgatc gccgacgcgg
    11581 tgtcgcccgc gccgtcgcgc gtgatgctcg ctcgcgccga ccagtgggcc gacgcggtga
    11641 ccggcggcgc gtacgccgcc agcagcggcg tgccgctcct gctcacgcag tccgacagcc
    11701 tccacccggc cgtggcggcg tggctcgcgg ccaagcgccc ctccgaggtg atcctgctgg
    11761 gcggcgagag cgcgctgtcg gccaacgtgg ccgcggccgt gccgggacgc gccacgcgcg
    11821 tgtccggccc aacccgcgcc ggcaccgccg tggcggtcgc gaagtccctg tggggccgcc
    11881 gcgacggcgc cccaggcgac cgcttcgtgg ccgtggacgg cttcgcctcc gactcctggt
    11941 cgccggcgct ggccgcgtcg gtgctgtccg ctcgtcaggc ggcgccccag atcctggtca
    12001 acggcggggc cccgagcacg gtgtcgccgg agacggaggc gtacctgacc gagctgggct
    12061 actcgtcgtc gaagtcggcc acggcctacc tggtgggccc gaaggtcggc gcgggcaccg
    12121 aggacagcct caacgcgctg ctcacccagt agcggtccca gcccgcgtta cacccggggt
    12181 cagcgctcgg ccagcaggtc cgcggtgacg tcggtgaccg ccggcgttcc acacagcgcc
    12241 atggcccgcg cgagctcgac ccggtagttc tccaagaccg cgcgcacgcc gtcggcgccg
    12301 tcgacggcca gcccccacag caccgggcgg cccagcagga ccgcccgcgc gcccagcgcc
    12361 agcgcccgca ccacgtcggt gccccggcgg atgccgccgt cgacgtacac ctccgcgcgc
    12421 tcgcccaccg cccgcaccac gtccggcagc gcctcggccg tggggattgc gccgtcgagc
    12481 tgccggccgc cgtggttgct caccacgatc gcgtccgcgc ccgccgccac gcaggcggcc
    12541 gcgtcgtcgc cgcgcagcac gcccttgacc accaggggca ggtcggacag gccgcgcagc
    12601 caccccaggt cgtcgaacgt cagggacgac tccacggcgt gctcggcgta ggcgcgcagc
    12661 gccgagccgt ccgtcggcac gtccgcggcg gacggcgtgt cgcccaggtt cgccttcacc
    12721 acgcccatgg gcagcgcgaa gttgttgcgc tcgtcgcggc ggcggtagcc caggaccggc
    12781 aggtccacgg tcagcacgat cgcccggtag ccgctcgccg ccgcccggcg caccagctcg
    12841 gcggtcaggc cgcggtcggt gtgcacgtac agctggaacc agcgcggcgc gtcgggcgcc
    12901 gccgcggcca cctcctccat cgcggtgttg gcgaacgtcg acacgaccat gaccgtgccg
    12961 aacgcggcgg cgccgctggc cgtggcggcc tcgccgtccg ggtgggccag gcgctggtac
    13021 gccgtgggcg ccaccaggac gggcgccgcc acggggacgc cgagcacgtg cgcctccgtg
    13081 gacaccgccg agacgtcgcg caggacgcgc gggcgcaagc gcagccgctc ccacgcggcc
    13141 agattctccc gcagcgtgcg ttcgtcgtcg gcgcctgaca cgtagtagtc ataggcgccc
    13201 gcgtgcaacg atgcgcgggc caggacctcg aggtcctcag gagtggatcc gggttcgacc
    13261 gccatgggct cgaaggctac ggcagcagga ggaaccatga ggcagcggcg aggcactcgc
    13321 ggacggctcg tcaccgcgct ggtggcgctg gtggcggggg cggcgctgct gccgctgctg
    13381 ccggcgacgg cggcgcccaa cggctacgtc gagatgagcg acggcgtgct gatcgcggtg
    13441 aacgtgcgcc cgccccgcaa cttcgtcgag gggcgccgct acccggccgt gttcgagatg
    13501 tcgggctacg acggcggctc ggccagcgac cagaccctga tgggcgacat cgccgacgcc
    13561 ttcggctacg agggcgaggt gccgttgcgc gaggactccc gccagctcac gcaccgcttc
    13621 gagggcgact acttcaccat ccacgcctcc gtgcgcggca ccggctgctc gggcggcacg
    13681 ttcgacctgt tcagctggcg cgcggcgctg gacggccacg aggtcatcga gtggatcgcc
    13741 cggcagccgt ggtcggacgg caacgtcgcc ctgatgggcc acagctacgg cggcatcacc
    13801 ggcttcatga tcgccgcgac gcgcccgccg cacctgcgcg ccgccacgct gtccgggctc
    13861 atcgacgacc tctaccgcgg catcacctac cccggcggcg tgtccaacta cggcttcccg
    13921 ctggcctgga ccggcgtcat ccgcccgttc tacgacctcg gcggcgggct cgcgccgggc
    13981 atcgtgcgca cgcaggaccc ggtgtgcgcg cagaaccagg cgagcaagaa ccgcaccgtg
    14041 ctggaggacc cgatcgtcaa cggcgtcggc gacacggact ccaactggtg gcgctcgcgc
    14101 tccctgatca gctacgtgca cctcatcgac gtgccgatgc acatcaccgg cgcctaccag
    14161 gacgagcaga ccgggccgcg cggcccgttc cacctgttcg agaagctgcc cacccacatc
    14221 ccgaggcgcc tcgtcatgac caacggcgac cacggcaccc agcagggccc ggacgccgtg
    14281 tgggacgacc gcgtggcgtg ggtcgaccag ttcacccgcg gcctgccgaa caggttcgat
    14341 cccatcccgg cacccggcgg cggcacgcgc gcgcacagct ccacgcgtgt gctgctggag
    14401 ttgcacagcg gcgtgcacga gggcagccag ggcacccacg acgagcgcca cccgaacggc
    14461 gtcgtggaca agaccgcctt ccccctggag aacacccagt ggacggacct gtacctgcgc
    14521 ggtggcggcg ccctgtccac cacgccgccg gcggaggcgg aggagcccgc ccggtacgtc
    14581 tccgggagcg ggcgccagtc ctggagctac caggccgggc ccgcggccgg ctcggagctg
    14641 tccaccccgg acggcccgga cgaggcggtg tacgccacgc cggcgttcac gcaggacgtg
    14701 ctgatcgcgg ggccgatggc ggccacgctg ttcgtggaca ccaccgcgcc cgacaccgag
    14761 ctgttcgtgc agctcatcga cgagggcccg gacggcagcc gcagctacct gcagcgcggc
    14821 atgctgcgcg ccagccaccg cgcactgaac gtcgcgatgt cggacaagac cgccgacggc
    14881 cgcatctacc ggccgttcca cccgcacacc aacccgacgc ccgtggtgcc cgggcaggtc
    14941 aacgagtacg tggtggagat cttcccagtc ggccacgtcc tgcgcgccgg ccaccgcctc
    15001 gtcgtgaagg tccacgcgcc accgcgagtg gacagcttca acgcctacgt gccccgctac
    15061 gcgccgtcgg tgaacaccat tcaccacggt ccgcggacac cgtcgcggct gctgctcccc
    15121 gtggtgccgc tggccggcgt gcgcctggga ccggagatcg cctgcggccg ccaggaggcc
    15181 gtgcgctgcg tcccgggcgg cgccgacggg agctgaccgg cgtctggcgt gcggctgacc
    15241 tggcccggac gggtcatgtc cgcccctggc cgggcggccc atgacgacag ccgcccggga
    15301 cgatcttgac ggtttccctg cccgtggggg cctgcacgat cccccgcttg gggcaccatc
    15361 ggcatcacga tgtcgacgcg tgctttcacg cgccggcgcg tccatatgct cgctgcac
//
LOCUS       DATMKC010000445         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_246841, whole genome shotgun sequence.
ACCESSION   DATMKC010000445 DATMKC010000000
VERSION     DATMKC010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246841"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..489)
                     /gene="ssb"
                     /locus_tag="VNU01_12915"
     CDS             complement(22..489)
                     /gene="ssb"
                     /locus_tag="VNU01_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWH33564.1"
                     /translation="MANDNVITIVGNLTDDPELRYTPNGVAVANCRVAVNRRMFNKES
                     NSWDDKLDGYFTINVWRDYAENVAESLSKGTRVLVTGRLISRSYEDKEGQTRWVTEIE
                     ADEICPSLRWATAKITKVNRSGGGGGGGGGGSETSWGGAPPPAQAPAADDVPF"
     gene            complement(570..887)
                     /gene="rpsF"
                     /locus_tag="VNU01_12920"
     CDS             complement(570..887)
                     /gene="rpsF"
                     /locus_tag="VNU01_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWH33565.1"
                     /translation="MRTYELMFISRGSLDEAAQQALSDRFTNLIAQQGGTVHKVDQWG
                     KRQFAYEIDHMTEGYYTVVEFDASPDGLNEVERQLKITDDIIRHKVVRPGARLKRQSK
                     PAA"
     gene            complement(1083..2054)
                     /locus_tag="VNU01_12925"
     CDS             complement(1083..2054)
                     /locus_tag="VNU01_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33566.1"
                     /translation="MRRARIAIVAPAVVVVALLVLSWAAGLLDGEAAVDEEAPRAAWL
                     THRWVGEPVDEEAVDRLARRLREARMTDIYVHVGPLDAHGGLTLQASPYAEEFAGAMH
                     RRLPDLRVQAWLGQAEARGGGLLDLRDDRAQAGVVRSAEGFLRRGYDGVHYDIEPIWD
                     GDRHFLALLEQTDRMLDRHPGAVLSVAAEEVPVFPGAERLIRTVVPRYHPWTIDFHRA
                     VARHADQIAVMTYDSAMPFRHLYERFVARNTRLLARALPTGTDLLVGVPSYQEWNAGH
                     WPAERVEAAARGLRRGLADLPPERRPRVGWALYGDWHTDEAEWRALP"
     gene            complement(2051..2176)
                     /locus_tag="VNU01_12930"
     CDS             complement(2051..2176)
                     /locus_tag="VNU01_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33567.1"
                     /translation="MSSRISACEPPVRFVDEQVRGPFASFRHEHRFEERDGGTST"
     gene            complement(2242..2868)
                     /locus_tag="VNU01_12935"
     CDS             complement(2242..2868)
                     /locus_tag="VNU01_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWH33568.1"
                     /translation="MGQVLSVCVYCASSEAVRPSYRAVAAELGAAIAVEGWALVYGGG
                     NIGLMGEVARAALAGGAHVTGIIPHRLATREIELRDVTELIRVETMRERKALMDERSD
                     AFVVLAGGIGTVEELVEMITLKQLGWHDRAIVVLDTEGFWDPLLAQFQRMVDEQLAEP
                     SLLELFQVTRDVASTVAALRDYEPPAPRPLTKAEVDERLEIVEDEPGP"
     gene            complement(2886..3263)
                     /locus_tag="VNU01_12940"
     CDS             complement(2886..3263)
                     /locus_tag="VNU01_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33569.1"
                     /translation="MSGLSRQERAALRGMLAARHPWLGHDEFGPRAVEAGECDRCGAE
                     ARLVATCGPIAWSALGRRCAAEVGADAWCEGHREDAERALAWLAGLPEAADHAARLWW
                     VATGEVRLDPELLELSRRVALPE"
     gene            complement(3260..>3521)
                     /locus_tag="VNU01_12945"
     CDS             complement(3260..>3521)
                     /locus_tag="VNU01_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33570.1"
                     /translation="TTGGDAGSDEPTMVGTGPDTPVSSDDLPGSDDPASVVHEPDAGE
                     PPVEETDTEPASASGLDGGLLAAIAAGLVGLVFAGWWLRRDA"
BASE COUNT          466 a         1329 c         1213 g          513 t
ORIGIN      
        1 tgggtgcagg tctcgtgggt gttagaaggg cacgtcgtca gcggcgggcg cctgtgccgg
       61 cgggggcgcg ccgccccaac tggtctcgct gccgccgcct ccgccgccac cgccgccgcc
      121 ggagcggttc accttggtga tcttggcggt cgcccagcgc aggctcgggc agatctcgtc
      181 cgcctcgatc tcggtgaccc agcgggtctg gccttccttg tcctcgtagc tgcggctgat
      241 cagacggccg gtgacgagga cgcgagtgcc cttggacagg gactccgcga cgttctccgc
      301 gtagtcgcgc cagacgttga tggtgaagta gccgtccagc ttgtcgtccc aggagttgga
      361 ctccttgttg aacatgcgcc ggttcaccgc gacccggcag ttggccacgg ccacgccgtt
      421 gggcgtgtag cgcagctccg ggtcgtcggt caggttgccg acgatggtga tcacgttgtc
      481 gttggccacg gcgtggtctc ctcgctcgcg tggtcccgcg cgtcgcgggc tggtcgcttc
      541 cgggtgggga agctcgtcgg ggaagaggcc taggccgcgg gcttggactg gcgcttcagg
      601 cgcgcgcccg gccgaacgac cttgtggcgg atgatgtcgt cggtgatctt gagctggcgc
      661 tccacctcgt tcagcccgtc aggggaggcg tcgaactcca cgaccgtgta gtagccctcg
      721 gtcatgtgat cgatctcgta ggcgaactgg cgcttgcccc actggtcgac cttgtgcacg
      781 gtgccgccct gctgggcgat caggttcgtg aaccggtcgg agagcgcctg ctgcgcggcc
      841 tcgtcgaggc tgccgcggga gatgaacatc aattcgtagg tacgcaagag cggaccatcc
      901 tctggtctag tggcccccgc tgccggatcc gccaggcacg gcgagtcccg ccaggagcag
      961 gacggaagcc gccacagtac cagccgctcg gacttccctc cacccggagc cagcacccta
     1021 gcggcccgct gcgacagcgc accccgccca tccacagtcg gcggagccgt gcccccgact
     1081 gctcacggca gggcgcgcca ttcggcctcg tcggtgtgcc agtcgccgta cagcgcccaa
     1141 cccacgcgcg gccgccgctc gggcgggagg tccgccagtc cccggcgcag tccgcgtgcg
     1201 gccgcctcga cgcgctcggc gggccagtgg ccggcgttcc attcctggta gctgggcacg
     1261 ccgacgagca ggtccgtgcc cgtgggcagc gcccgtgcga gcaggcgcgt gtttcgcgcg
     1321 acgaagcgct catagaggtg ccggaacggc atggcgctgt cgtaggtcat gaccgcgatc
     1381 tggtcggcgt ggcgggcgac ggcgcggtga aagtcgatcg tccacgggtg atagcgcggc
     1441 accacggtgc ggatcagccg ctcagcccca gggaacaccg gtacttcctc ggcggccacg
     1501 gacagcaccg cgcccggatg gcgatcgagc atgcggtcgg tctgctccag cagcgccagg
     1561 aagtggcggt ccccgtccca gatcggctcg atgtcgtagt gcacgccgtc gtagccgcgc
     1621 cgcaggaacc cctcggcgct tcggacgacc ccggcctgcg cgcggtcgtc gcgcaggtcg
     1681 agcagcccgc ctccgcgcgc ctccgcctgc ccgagccagg cctgcacgcg cagatccggg
     1741 aggcggcggt gcatcgcccc ggcgaactcc tcggcgtacg gcgacgcctg aagcgtcagg
     1801 ccgccgtggg cgtccagcgg accgacgtgc acgtagatgt ccgtcatgcg ggcttcgcgc
     1861 aggcgccgtg ccaggcgatc gaccgcctcc tcgtccacgg gctcacccac ccagcgatgg
     1921 gtcagccacg ccgcgcgggg cgcctcctca tccacggcgg cttcgccgtc cagcaggccg
     1981 gcagcccatg acaacaccag cagcgccacc accactactg ctggcgccac gatcgcgatc
     2041 cgagcacgcc tcatgtgctc gtgccgccgt cgcgctcctc gaagcgatgc tcgtggcgga
     2101 aggacgcgaa cggcccgcgc acctgctcgt cgacgaatcg caccggaggt tcacacgcgc
     2161 tgatgcggct gctcatcgtc catggcagtc cccagtgccg ggcccgccac gtgaccgtgt
     2221 cgcccaagtc gagtctctcc gtcaggggcc gggctcgtcc tccacgatct ccaggcgctc
     2281 gtcgacctcc gccttggtga gcgggcgcgg cgccggcggc tcgtagtccc gcagcgcggc
     2341 gaccgtcgag gcgacgtcgc gcgtcacctg gaacagctcc agcaggctcg gctcggcaag
     2401 ctgctcgtcc accatgcgct ggaactgcgc cagcagcggg tcccagaagc cctccgtgtc
     2461 cagcaccacg atggcccggt cgtgccagcc gagctgcttg agcgtgatca tctcgaccag
     2521 ctcctccacg gtgccgatcc cgcccgccag caccacgaac gcgtcactgc gctcgtccat
     2581 cagcgccttg cgctcgcgca tggtctcgac gcggatcagc tcggtgacgt cgcgcagctc
     2641 gatctcgcgc gtcgccaggc ggtgcgggat gatcccggtc acatgggcgc cgccggcgag
     2701 cgccgcacgc gccacctcac ccatcaggcc gatgttgccc ccgccgtaca ccagcgccca
     2761 gccctcgacg gcgatggcgg cgcccagctc cgcggccacc gcgcgataac tcggccgcac
     2821 cgcctcgctg gaggcgcagt acacgcacac ggacagaacc tggcccatgg cggcgatgct
     2881 acccgtcact ccggcagcgc cacgcgccgg gacagctcca gcagctccgg gtccaggcgc
     2941 acttcgccgg tggccaccca ccacaggcgg gcggcatggt cggcggcctc gggcaggccc
     3001 gcgagccagg ccagcgcccg ctcggcgtcc tcacggtggc cctcgcacca tgcgtcggcg
     3061 ccgacctcgg ccgcgcagcg gcgcccgagc gcgctccagg cgatcggccc gcacgtggcg
     3121 accaggcgcg cctccgcccc gcagcggtcg cactcgcccg cctcgaccgc ccggggcccg
     3181 aactcgtcgt ggcccagcca gggatggcgc gcggcgagca tgcctcgcag tgccgcgcgc
     3241 tcctgtcggc tcagtccgct cacgcgtcgc gccgcagcca ccatccggcg aagaccagtc
     3301 cgaccaatcc cgccgcgatg gcggcgagca ggccgccgtc caggccactg gcggaggcgg
     3361 gctccgtgtc ggtctcctcc accggcggct cgccggcgtc cggctcgtgc acgacgctgg
     3421 ccgggtcgtc ggaaccgggc aggtcgtcac tggagacggg cgtgtccggg ccggtgccca
     3481 ccatggtcgg ctcgtccgag ccggcgtcgc cgccggtggt t
//
LOCUS       DATMKC010000446         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_275661, whole genome shotgun sequence.
ACCESSION   DATMKC010000446 DATMKC010000000
VERSION     DATMKC010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275661"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VNU01_12950"
     CDS             <1..598
                     /locus_tag="VNU01_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33571.1"
                     /translation="AVVGAAPLDFGVRRRMDTVVNLVAEPGDAALRLLMADVLGWLGD
                     IGAAVVNGLDAYVVGSSPARQLALFAHLGLSHPPARVLRDPAAVGVALAELGLAEADA
                     EVLDGPPAVVRERVGGRRVRLEFAGGRLVGDDAGLPEVVVAAAAEVLGTAAVEFGGVT
                     LARDGERVWWLGADTRPGVSPAALGALAEHVRFRAGLG"
     gene            complement(611..1000)
                     /locus_tag="VNU01_12955"
     CDS             complement(611..1000)
                     /locus_tag="VNU01_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33572.1"
                     /translation="MNASDERNIRAERVALGISLAVVLAVLAGIGSLMLDRGPGELRF
                     TLTMADVTAAGDEHHVRVRVRNDGPRTAENVQVVAELRLPGQEPSEGEQTVQFLAGGA
                     EEELVFVFADDPADGELTLRVASYTLP"
     gene            complement(997..1860)
                     /locus_tag="VNU01_12960"
     CDS             complement(997..1860)
                     /locus_tag="VNU01_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HWH33573.1"
                     /translation="MAAEQDSAAGVELRDAVRGFSGGMLFGVPLLYTMEVWWVGGHTT
                     PAQMLLVLAFTFAVVLALNVTGGFRGRDDGTAAGILQDSLEALAIGLVSSAVVLVVLQ
                     EIRPGVPLSVALGKVIYEAMPFAMGVSLARHFLQGGRVDPDGEAGTEPELASRVNATV
                     ADLGAAAVGAIFVALNIAPTDEVPMLTAAMPPLWLIALVVLSLVSTYLIVFAAGFGDV
                     DARRQQQGVLQHPWTETLASYLVALVVAGVLLALFQQFDDKDPWQDMLSQVVVLGLPA
                     SVGGAAGRLAV"
     gene            complement(1884..>2280)
                     /locus_tag="VNU01_12965"
     CDS             complement(1884..>2280)
                     /locus_tag="VNU01_12965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWH33574.1"
                     /translation="PDALPGLAALRGAGLRVAVVANQPASRTAELRALGVDAEVMAMS
                     DELGVAKPDPAFFARVLELVDAPAERVAYVGDRVDNDVLPALRAGLRPVWLRRGPWAA
                     LQRLPAGVAVTEARGLPELAGRFAPPVSG"
BASE COUNT          286 a          905 c          834 g          255 t
ORIGIN      
        1 cgccgtggtg ggcgccgcgc cgttggactt cggcgtgcgc cggcgcatgg acacggtggt
       61 gaacctggtc gccgagccgg gcgacgcggc cctgcggctg ctcatggccg acgtgctcgg
      121 ctggctgggc gacatcggcg cggccgtggt caacggcctg gacgcctacg tggtgggctc
      181 cagcccggcg cgccagctcg cgctgttcgc gcacctgggg ctgtcccacc cgccggcgcg
      241 cgtgctgcgc gacccggcgg ccgtgggggt ggccctggcc gagctgggcc tggcggaggc
      301 ggacgccgag gtgctcgacg gcccgcccgc cgtggtgcgc gagcgcgtgg gcggccggcg
      361 cgtccgcctg gagttcgcgg gcggccggct ggtgggcgac gacgccgggc tgccggaggt
      421 ggtggtggcc gccgcggcgg aggtgctggg caccgcggcg gtggagttcg gcggcgtgac
      481 gctggcgcgc gacggcgagc gcgtgtggtg gctgggcgcc gacacgcgcc cgggggtctc
      541 gccggcggcg ctgggcgccc tggccgagca cgtccgcttc cgcgccggcc tcggctgacc
      601 ccaggagagg tcagggcagc gtgtaggagg cgacgcgcag ggtcagctcg ccgtcggcgg
      661 ggtcgtcggc gaagacgaag accagctcct cctccgcgcc gccggccagg aactgcaccg
      721 tctgctcgcc ctcggacggc tcctgcccgg gaaggcgcag ctccgccacg acctgcacgt
      781 tctcggcggt gcgcggcccg tcgttgcgca cccggacccg cacgtggtgc tcgtcgcccg
      841 cggcggtgac gtccgccatc gtcagcgtga agcgcagctc gcccggcccg cggtccagca
      901 tcagcgagcc gatgcccgcc aggaccgcca gcaccaccgc caggctgatg cccagcgcga
      961 cgcgctccgc gcggatgttg cgctcgtcgc tcgcgttcac accgccagcc ttcccgccgc
     1021 gccgcccacg gaggccggca gccccaggac cacgacctgg ctcagcatgt cctgccacgg
     1081 gtccttgtcg tcgaactgct ggaacagcgc cagcagcacg cccgcgacca ccagcgccac
     1141 cagatacgac gccagcgtct ccgtccacgg gtgctgcagc acgccctgct gctggcggcg
     1201 cgcgtcgacg tcgccgaacc cggcggcgaa cacgatgagg taggtcgaga ccagggacag
     1261 caccaccagc gcaatcagcc acagcggcgg catcgcggcg gtgagcatcg gcacctcgtc
     1321 ggtcggcgcg atgttgagcg ccacgaagat cgcgcccacc gcggcggccc ccaggtccgc
     1381 cacggtcgcg ttcacccgcg aggccagctc cggctccgtc cccgcctcgc cgtccgggtc
     1441 gacccgcccc ccctggagga agtggcgcgc cagcgagacg cccatggcga acggcatcgc
     1501 ctcgtagatc accttgccca gcgccacgct caacggcacg ccggggcgga tctcctgcag
     1561 caccaccagc acgacggcgg acgacaccag gccgatggcc agcgcctcca agctgtcctg
     1621 caggatgccg gccgccgtgc cgtcgtcgcg gccgcggaag ccgcccgtga cgttcagcgc
     1681 cagcaccacg gcgaacgtga acgccagcac cagcagcatc tgcgcgggcg tggtgtggcc
     1741 gccgacccac cacacctcca tggtgtacag cagcggcacg ccgaacagca tgccgccgga
     1801 gaagccgcgc accgcgtcgc gcagctccac gcccgccgcg ctgtcctgct ccgccgccat
     1861 gtgccgcctc ctcgggggtg ctcctacccg gaaaccggcg gcgcgaagcg gcccgcgagc
     1921 tcgggcaggc cccgcgcctc ggtgaccgcg acgcccgccg gcaggcgctg cagcgccgcc
     1981 cacggcccgc gccgcagcca caccggccgc aggccggcgc gcagcgccgg cagcacgtcg
     2041 ttgtcgacgc ggtcgcccac gtaggccacg cgctcggccg gcgcgtccac cagctccagc
     2101 acgcgggcga agaaagccgg gtcgggcttg gcgacgccca gctcgtcgct catcgccatc
     2161 acctcggcgt ccacgcccag cgcgcgcagc tccgcggtgc gcgacgccgg ctggttcgcg
     2221 accaccgcga cgcgcaggcc cgccccgcgc agcgcggcca ggccgggcag cgcgtccggg
//
LOCUS       DATMKC010000447         8088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_198476, whole genome shotgun sequence.
ACCESSION   DATMKC010000447 DATMKC010000000
VERSION     DATMKC010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198476"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VNU01_12970"
     CDS             <1..658
                     /locus_tag="VNU01_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33575.1"
                     /translation="ASAAPARRGRCRATAPARTLGLHVAAARETFEESGLLLATHADG
                     SPPDAEAPEVTAMRVRLADRDDPCGGEEFNAWLRERGLVLELGRLAYWARWITPTWER
                     RRYDTRFFLARAPEGQVAGFDDVEVTGQLWVTPAEALEQAASGTLHMIYPTIKNLEEL
                     AEACGGQAGVDAAFSYAASREVQTVLPHFERTDDGGFRVLHPGEPGYPAERYAADLAR
                     "
     gene            660..1493
                     /locus_tag="VNU01_12975"
     CDS             660..1493
                     /locus_tag="VNU01_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWH33576.1"
                     /translation="MDRLDDLVHRVLAPNPGPMTLEGTNTYLVGAPATGEVVVVDPGP
                     DLAEHRGAVEQGAAALDARIAGVVLTHHHRDHAQAAGWAHDWDVPLWAFAPDLVAKAA
                     PSATPAALADGRTLELAGVRLQAVHTPGHSSDHLCLRVMDTDVVLTGDHVLGRGTTVV
                     VWPDGDMADYMASLRRVAALPATALYPGHGPALADPAAKVREYLAHRDERERQVLDAL
                     RAGDDAPMAVVKRVYADVDEALHGAAARSVQAHLEKLVREGAVVRDGDPREEGTTYRV
                     R"
     gene            1483..2343
                     /locus_tag="VNU01_12980"
     CDS             1483..2343
                     /locus_tag="VNU01_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWH33577.1"
                     /translation="MSDDPTRPLGPDDRTRPLAPQDETRPLNAMGAGGRLDDERASRA
                     RTAMALAAIAVLLLVVVLLLVNRGGDGDSTTVGSSPTETSTEDEPTDSPSPTPTETES
                     PTEEPDEPREPTDTDAAEFSAAYRPQGAQAVQSATADLDGDGQNEVVFASIHSDSARI
                     DIARWDGREYQIVYTGQGGGAERLDDFRVADYTGDGTREVVTVQSVGAQGQSLSIWGW
                     NGQAFAPQRATGGCWNGSHTFGIIGASIERGRIRASCDGSPLPAAAWPSDVYVWDADG
                     GGWVYDHTEE"
     gene            2347..2730
                     /locus_tag="VNU01_12985"
     CDS             2347..2730
                     /locus_tag="VNU01_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWH33578.1"
                     /translation="MPYRTVLVGTDGSLTAARAVQRAVSLAAATSARLVVAYVGDPAT
                     GRQVLEAVAQQHADAGVELVHKLLEGDPADALLGLAAAEHADVVVVGNKGMTGAQRFL
                     LGSVPNKVSHQARCDVLIVNTSARD"
     gene            2893..4443
                     /locus_tag="VNU01_12990"
     CDS             2893..4443
                     /locus_tag="VNU01_12990"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWH33579.1"
                     /translation="MAVELPKSKSVLANPSQEELKELVAKMPNAQLTEFGNYNVKCRV
                     TARSAGSTFIVTDDAESTTKKSITRARYDDVVRMQEAYIADKDMVLVEGYIGPENSPL
                     KRPARVFIEASNANIPAMQQQLYYPKDADWREEDALTVIYTPNLVAEGWPDDRLVTID
                     LENWVTRVFNIDYFGESKMGGLRMWMEWAYQQGALAMHAGAKVIPTDNGDKVALIVGL
                     SGTGKTTTTFTRQNGSLPVQDDIVGFVNGGDVFSTENGCFAKTYGLDPQYEPTIHGAL
                     SSPEGWLENVAVDGGGKVDYFDDSYTANGRGTFSLSQIDHYDPRKLGKGDFLLILNRN
                     ESIIPAVAKMTSKEQAVAYFMLGETKGTSAGGAGEDGKFLRVPGTNPFFMWDDALQGN
                     RLLELLDTLDYDFGVYLMSTGRVGGGEDQPGSKKVKIPHSSAIVKGIAEGTIEWVEDP
                     DFGYLVAASVPGIDAEDEDLLRPRELYSKQGRGEEFDRLVDNLKAERREYMAQYPQLA
                     PAIIEALG"
     gene            complement(4496..5911)
                     /locus_tag="VNU01_12995"
     CDS             complement(4496..5911)
                     /locus_tag="VNU01_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HWH33580.1"
                     /translation="MDFVGRTRELALLARELSRVQASMGTAAPGRCLLVRGRRRVGKS
                     RLIEAFVGTQTAPTLFFTASRQGDAELGHFAGEAARSTLPGATLFEGLALADWDAALR
                     LLHAALPDDRVSIVVIDEFPYLLEGRRDVEATFQKQWDRLLSRKPVLLLLIGSDLAVM
                     EALNTHGRAFYQRGSEFVVRPLSPVETGAIVDSPSAADAFDAYLVSGGMPLVCRDWPR
                     GASLWEFLSQSLSDETSALVVSAHRTLSAEVPEQYSAGAVLRAIGNGERTFANIARAS
                     GLAGSSLSVALETLIQKRIVAKDVPLSTKPSRESRYRIRDPYLRFWLTFVEGSMPEIE
                     RGRGDRVVDRIRASWSSWRGAAVEPVVREAIRRLAPLDGLPQADAVGGYWTRSNDPEV
                     DIIGGDREPVARVIHFAGSVKWRDEAAFEQTDLHRLAALVARVPGASPETPLLVVSRS
                     GVRARGMACALGPEELLEAWR"
     gene            complement(5978..7468)
                     /locus_tag="VNU01_13000"
     CDS             complement(5978..7468)
                     /locus_tag="VNU01_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33581.1"
                     /translation="MRAPSRRRLAAAMAAVAVLASLAAAAGIPGRATYGARASADEPY
                     YLLSALAFATDGALDITDELAEERWRPFHESRLPQQTEPLPDGRRVAPHDPLLSVLLA
                     PGMWWGTWLGGADLGATLAKAELALLAGVLAALLLWVAVRRLGVSLRVSAVAVALFGA
                     SAPLAAYGHQVYPELPLALCVVIAVAALTGPFQRGAAVGLLLAVVAMPWLAVKSVPVA
                     AVLALLGLARLWRRGQRPAAAALAVAFAAAGAAYLGVYRELYGGWTAYAAGDHFVNGE
                     FTAVGTRVDVLGRSRRLVGLFVDRGFGIAAWQPAWLLAIPAGAALTRRRPAHWLVLAL
                     PLLAGYLNATFVAYTMQGWWFPGRQLVMVLPLAVLIIAWWADQLGRRTLAVLATLGGL
                     GVWSYVWLAGQVAAGRLTWIVDFFGTRDPWYRMWSPLLPNYLRVTDSTWVLHAEWIVV
                     LLALAWAGLRSMGKLSRPGFRRRPRRARLAVRPPAFLESDREDNAT"
     gene            complement(7489..>8088)
                     /locus_tag="VNU01_13005"
     CDS             complement(7489..>8088)
                     /locus_tag="VNU01_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWH33582.1"
                     /translation="ATVSMAAALAAVLVVLPLAYLTVRRGGWAAGVANAVVTGGFALP
                     GLVVALALVSWSVPAASPLAPLYQTHTLLVTAYVVHFGAQALRTAQVSLGAMPPRVED
                     AARMLGAGRLRRLVRVELPLMAPGLLAGAGLVLLSAMKELPATLLLAPPGFQTLATTV
                     WTATEDAFLAEASLAAILLVLLSAGLTWVLVLRQSDAID"
BASE COUNT         1286 a         2970 c         2868 g          964 t
ORIGIN      
        1 ggcgagcgcg gcgccagctc gccggggtcg atgccgcgcc actgcgccgg cgcgcaccct
       61 tgggctgcac gtcgcggcgg cgcgcgagac cttcgaggag tccggcctgc tgctggccac
      121 gcacgccgac ggctcgccgc cggacgccga ggccccggag gtcacggcga tgcgcgtccg
      181 tctggccgac cgcgacgacc cctgcggcgg cgaggagttc aacgcctggc tgcgcgagcg
      241 gggcctggtc ctggaactcg gccgcctggc gtactgggcg cgctggatca ccccgacctg
      301 ggagcgccgc cgctacgaca cgcgcttctt cctggcgcgc gccccggagg ggcaggtggc
      361 tggcttcgac gacgtcgagg tcaccggcca gctctgggtg acgccggccg aggcgctgga
      421 gcaggcggcc tccggcacgc tgcacatgat ctaccccacc atcaagaacc tggaggagct
      481 ggcggaggcg tgcggggggc aggccggcgt cgacgcggcg ttctcctacg ccgcctcccg
      541 ggaggtgcag acggtgctgc cgcacttcga gcgcaccgac gacggcggct tccgggtgct
      601 gcacccgggc gagcccggct acccggcgga gcgctacgcc gcggatctgg cgcgctgacg
      661 tggaccgcct cgacgacctg gtccaccgcg tgctggcgcc caacccgggc cccatgacgc
      721 tggagggcac caacacctac ctggtgggcg cgcccgccac gggcgaggtc gtggtggtgg
      781 acccgggtcc ggacctcgcg gagcaccggg gcgccgtgga gcagggggcg gccgccctcg
      841 acgcccgcat cgccggcgtg gtgctgaccc accaccatcg cgaccacgcg caggccgccg
      901 gctgggcgca cgactgggac gtgccgctgt gggcgttcgc cccggacctc gtcgccaagg
      961 cggcgccgtc ggcgacgccg gccgcgctgg ccgacggccg gacgctggag ctggccggcg
     1021 tgcgcctcca ggcggtgcac acccccgggc actccagcga ccacctgtgc ttgcgggtga
     1081 tggacaccga cgtggtgctc accggcgacc acgtgctggg ccggggcacc accgtggtgg
     1141 tgtggccgga cggcgacatg gccgactaca tggcctcgct gcggcgcgtg gccgccctgc
     1201 ccgccaccgc gctgtacccc ggccacggcc cggcgctggc cgacccggcc gcgaaggtgc
     1261 gcgagtacct ggcgcatcgc gacgagcggg agcggcaggt actggacgcg ctgcgcgcgg
     1321 gcgacgacgc tcccatggcg gtcgtgaagc gcgtgtacgc cgacgtcgac gaggcactgc
     1381 acggcgccgc ggcgcgtagc gtgcaggcac acctggagaa gctggtccgg gagggcgccg
     1441 tcgtccgcga cggcgatccc cgggaggaag ggacgaccta ccgtgtccga tgaccccacc
     1501 cgcccgctcg gccccgacga ccgcacacgg cccctggcgc cgcaggacga gacgcggccg
     1561 ctgaacgcca tgggtgccgg gggccggctc gacgacgagc gcgcctcgcg cgcccggacc
     1621 gccatggcgc tggcggccat cgccgtgctc ctgctcgtgg tggtgctgct gctggtcaac
     1681 cgcggcggcg acggcgactc cacgacggtg gggtcgtcgc cgacggagac ctccaccgag
     1741 gacgagccga ccgacagccc gtcgcccacg ccgacggaga ccgagtcgcc cacggaggag
     1801 cccgacgagc cgcgcgagcc caccgacacc gacgcggccg agttcagtgc cgcctaccgt
     1861 ccgcaggggg cccaggccgt gcagtcggcc accgcggacc tcgacggcga tgggcagaac
     1921 gaggtggtgt tcgcctccat ccactccgac agcgcgcgca tcgacatcgc ccgctgggac
     1981 ggccgggagt accagatcgt gtacacgggc cagggtggcg gcgcggagcg gctcgacgac
     2041 ttccgcgtcg ccgactacac cggcgacggc acgcgcgagg tcgtgaccgt ccagtccgtc
     2101 ggcgcgcagg gccagagcct gtccatctgg ggctggaacg gccaggcgtt cgcgccgcag
     2161 cgcgccaccg gcggctgctg gaacggctcg cacacgttcg gcatcatcgg agcctcgatc
     2221 gagcgcggcc gcatccgcgc cagctgcgac gggtcaccgc tgccggccgc ggcctggccc
     2281 agtgacgtgt acgtctggga cgccgacggc ggcggctggg tctacgacca cacggaggag
     2341 taggcgatgc cctaccgcac ggtgctcgtc ggcacggacg ggtcgctcac ggcggcgcgc
     2401 gccgtgcagc gggcggtcag cctggccgcc gccaccagcg cgcggctggt cgtggcctac
     2461 gtcggcgacc ccgcgaccgg gcggcaggtg ctcgaagcgg tggcgcagca gcacgccgac
     2521 gccggcgtgg agctggtgca caagctgctg gagggcgacc cggccgacgc gctgctgggc
     2581 ctggccgcgg ccgagcacgc cgacgtggtg gtggtcggca acaagggcat gaccggcgcg
     2641 cagcgcttcc tgctgggctc ggtgcccaac aaggtcagcc accaggcgcg ctgcgacgtg
     2701 ctcatcgtca acacctcggc acgcgactga cgcgtccccc agcggccggg gcgggggcgc
     2761 gctcaagacc cgattgtcta cggatcctta ccgccgggta acatcgagcg accgccaggc
     2821 gggctccccg ctgtgggggg aatccgtttc cgccagaccg ggacaacgtc gtcccaggag
     2881 gaccgaagcg ccgtggccgt cgagctcccg aagtcgaagt ccgttctcgc caatccctcg
     2941 caggaagagc tcaaggagct cgtggcgaag atgccgaacg cccagctcac cgagttcggc
     3001 aactacaacg tgaagtgccg cgtgacggcg cgctcggccg gctccacctt catcgtcacc
     3061 gatgacgcgg agtccaccac caagaagtcg atcacccgcg cgcgctacga cgacgtcgtg
     3121 cgcatgcagg aggcctacat cgccgacaag gacatggtcc tggtcgaggg ctacatcggt
     3181 ccggagaact ccccgctgaa gcgcccggcg cgcgtgttca tcgaggcgag caacgccaac
     3241 atcccggcga tgcagcagca gctgtactac cccaaggacg cggactggcg ggaagaggac
     3301 gcgctcacgg tgatctacac gcccaacctc gtggcggagg gctggcccga cgaccggctg
     3361 gtcaccatcg acctggagaa ctgggtcacg cgggtgttca acatcgacta cttcggcgag
     3421 tcgaagatgg gcggcctgcg catgtggatg gagtgggcct accagcaggg cgccctggcg
     3481 atgcacgccg gcgccaaggt gatccccacg gacaacggcg acaaggtcgc gctgatcgtg
     3541 ggcctgtccg gcaccggcaa gaccaccacc acgttcacgc gccagaacgg ctcgctgccc
     3601 gtccaggacg acatcgtcgg cttcgtcaac ggcggcgacg tgttctccac ggagaacggc
     3661 tgcttcgcca agacctacgg cctggacccg cagtacgagc ccaccatcca cggtgcgctg
     3721 agctcgccgg agggctggct ggagaacgtc gcggtcgacg gcggcggcaa ggtcgactac
     3781 ttcgacgaca gctacaccgc caacgggcgt ggcacgttct cgctgtcgca gatcgaccac
     3841 tacgacccgc gcaagctcgg caagggcgac ttcctgctga ttctcaaccg caacgagtcg
     3901 atcatcccgg ccgtggccaa gatgaccagc aaggagcagg cggtcgccta cttcatgctg
     3961 ggcgagacca agggcacgtc ggccggcggc gccggcgagg acggcaagtt cctgcgcgtg
     4021 ccgggcacca acccgttctt catgtgggac gacgcgctgc agggcaaccg cctgctggag
     4081 ctgctcgaca cgctggacta cgacttcggc gtgtacctga tgtccaccgg ccgcgtcggc
     4141 ggcggcgagg accagccggg ctccaagaag gtgaagatcc cgcactcctc ggccatcgtg
     4201 aagggcatcg ccgagggcac catcgagtgg gtcgaggacc ccgacttcgg ctacctggtc
     4261 gcggcctcgg tgcccggcat cgacgctgag gacgaggacc tgctgcgccc gcgcgagctc
     4321 tactccaagc agggccgcgg cgaggagttc gaccggctgg tcgacaacct caaggccgag
     4381 cggcgcgagt acatggccca gtacccgcag ctggcccccg cgatcatcga ggcgctgggc
     4441 taggacgggc acgagacgca cgacgagggg cggccaccgg ccgcccctcg cgcgttcaac
     4501 gccaggcttc cagcaactct tcgggaccga gggcgcacgc cataccccgc gcccgcacgc
     4561 cggagcggga caccaccaat aatggtgttt cggggctggc gccaggtacg cgcgcgacaa
     4621 gtgcggccag ccgatgcagg tcggtctgtt cgaacgcagc ctcgtcccgc cacttcaccg
     4681 agcccgcgaa atgaatcact cgggcgacgg gttcccggtc accgccgatg atgtccactt
     4741 cgggatcgtt ggagcgggtc cagtagccac ccacagcgtc cgcttggggc aacccatcca
     4801 atggggccag ccgcctgatg gcttcgcgga cgaccggctc caccgcggcg ccacgccagg
     4861 acgaccatga cgcccggatg cggtccacta cccggtcgcc tcgacccctc tcgatctcgg
     4921 gcatggaacc ttcgacgaag gtcagccaga atcggaggta cggatcgcgg atgcggtaac
     4981 ggctctctcg gctcggcttg gtggacaagg gaacgtcctt ggccacgatg cgtttctgga
     5041 tcagggtctc cagggccacg ctgaggctgc tacccgccag accgctggcg cgcgcgatat
     5101 tggcgaaggt gcgttcacca ttccctatcg cccgcaacac cgcgccggca ctgtattgct
     5161 cgggcacctc ggcactcagg gtgcggtgag cgctcacgac caatgccgaa gtctcgtcac
     5221 tcagtgactg gctcaagaat tcccagagag atgcaccgcg aggccagtct cggcagacga
     5281 gaggcatgcc accgctgacc agataggcat cgaatgcatc cgccgcgctc gggctgtcca
     5341 cgattgcgcc ggtctccacc gggctcaatg ggcgtacgac gaactcgctg cctcgttggt
     5401 agaaggctcg cccatgggtg ttgagcgcct ccatgacggc aaggtcagag ccgatgagca
     5461 ggaggagaac cggcttgcgc gacagcaacc gatcccactg tttctgaaag gtggcttcca
     5521 catcacggcg gccctccagt aggtagggga actcgtcgat cacgacgatg ctgactcggt
     5581 cgtcgggcag tgcggcgtgc aggaggcgca aggcggcatc ccaatccgcg agcgcgagac
     5641 cctcgaacag cgtcgcgccg ggcaacgtgg agcgggccgc ttcgccggca aaatggccca
     5701 gctcggcgtc gccctgcctc gatgcggtga agaacagtgt cggagccgtc tgcgtgccga
     5761 cgaacgcctc gatcagccgg gacttgccca ccctgcgacg accgcgcacc agcaggcatc
     5821 gccctggcgc cgctgttccc atggaggcct gtactcgact cagctcacga gccagcaggg
     5881 ccaactcacg cgtacgcccg acgaaatcca tgcgccaaga atagtacagt acaccgtaca
     5941 tatcaatgct gaagttcagt gcactgggct tttatggtca ggtggcgttg tcctcgcggt
     6001 cggactccag gaaggccggc ggccggaccg ccaggcgggc gcgccggggg cgccgccgga
     6061 agcccggccg ggacagcttt cccatcgagc gcaggcccgc ccacgccagg gcgagcagca
     6121 ccacgatcca ctcggcgtgc agcacccagg tcgagtcggt gacgcgcagg tagttgggca
     6181 gcagcggcga ccacatccgg taccaggggt cgcgcgtgcc gaagaagtcg acgatccagg
     6241 tcagccgccc ggccgcgacc tgcccggcca gccacacgta gctccacacc cccaggccgc
     6301 ccagcgtggc gagcacggcc agcgtgcgcc gtccgagctg gtcggcccac cacgcgatga
     6361 tcagcaccgc cagcggcagc accatcacca gctggcgccc cgggaaccac cagccctgca
     6421 tggtgtaggc gacgaacgtg gcgttgaggt agccggccag cagcggcagc gccagcacca
     6481 gccagtgcgc gggccggcgg cgcgtcagcg cggcgcccgc cgggatcgcc agcagccacg
     6541 ccggctgcca cgccgcgatg ccgaagccgc ggtcgacgaa caggcccacc agccgcctgc
     6601 tgcgccccag cacgtccacg cgcgtgccga ccgcggtgaa ctcgccgttg acgaagtggt
     6661 cacccgccgc gtaggcggtc cagccgccgt acagctcccg gtacacgccc aggtacgccg
     6721 cgccggccgc ggcgaacgcg acggccaggg ccgcggcggc cggccgctgg ccgcggcgcc
     6781 acagccgcgc cagccccagc agcgccagca ccgccgccac gggcacgctc ttgaccgcca
     6841 gccacggcat cgccaccacc gccagcagca ggcccaccgc agcgccgcgc tggaacggtc
     6901 cggtcagcgc ggccacggcg atcaccacgc acagcgccag gggcagctcc gggtagacct
     6961 ggtgcccgta ggcggccagc ggcgccgacg cgccgaacag cgccacggcc accgccgaca
     7021 cgcgcaggga cacgcccagg cgccgcaccg ccacccacag cagcagcgcg gccagcacgc
     7081 cggccagcag cgcgagctcc gccttggcca gggtggcgcc caggtccgcg ccgccgagcc
     7141 aggtgcccca ccacatcccg ggggccagca gcacgctcag cagcgggtcg tgcggcgcga
     7201 cgcgccgccc gtcgggcagc ggctcggtct gctggggcag ccgcgactcg tggaacggcc
     7261 gccagcgctc ctcggccagc tcgtcggtga tgtcgagggc gccgtcggtg gcgaaggcca
     7321 gcgccgacag caggtagtag ggctcgtcgg cggacgcgcg cgcgccgtag gtggcgcggc
     7381 cggggatgcc cgcggcggcc gccagggacg ccagcacggc caccgcggcc atcgccgcgg
     7441 ccagccgccg tcgtgagggc gcccgcacgg tcagcgcgtg cctttcgctc agtcgatcgc
     7501 gtcggactgc cgcagcacca gcacccacgt gaggccggcc gacagcagca cgaggagtat
     7561 cgcggcgagg ctcgcctcgg ccaggaaggc gtcctcggtg gccgtccaca cggtggtggc
     7621 cagcgtctgg aagcccggcg gtgcgagcag cagcgtcgcc ggcagctcct tcatggccga
     7681 cagcagcacg agcccggcgc cggccagcag gcccggcgcc atgagcggca gctcgacgcg
     7741 gaccaggcgg cgcagccggc cggcgccgag catgcgcgcg gcgtcctcca cgcggggcgg
     7801 catcgcgccg agcgacacct gcgcggtgcg cagcgcctgc gcgccgaagt gcaccacgta
     7861 cgcggtcacc agcagcgtgt gcgtctggta cagcggcgcc agcggcgagg cggccggcac
     7921 gctccaggac accagggcca gcgcgaccac cagtccgggc agcgcgaagc cgccggtgac
     7981 cacggcgttg gcgaccccgg cggcccagcc gccgcggcgc accgtcaggt acgccagcgg
     8041 cagcaccacc agcacggcgg cgagcgcggc ggccatgctc accgtggc
//
LOCUS       DATMKC010000448         4945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_250894, whole genome shotgun sequence.
ACCESSION   DATMKC010000448 DATMKC010000000
VERSION     DATMKC010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250894"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="rpsB"
                     /locus_tag="VNU01_13010"
     CDS             complement(<1..681)
                     /gene="rpsB"
                     /locus_tag="VNU01_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWH33583.1"
                     /translation="MRQLLEAGVHFGHQTRRWNPKMKRFIHGERNGIYIIDLQQTIGM
                     LEQAYEHVRDTVAKGGTILFVGTKKQAQEAIETQATRVGMPYVNYRWLGGMLTNFETI
                     KTRLARLRELEEKERSGEFELLPKKEVLQLQREREKLERNLGGLRGMTKPPSAIWVVD
                     TVKEHIAVKEANRLNVPVVAVVDTNCDPDVIDFIIPGNDDAIRSGALLTRLIADACAE
                     GYQMRASRS"
     gene            complement(904..1803)
                     /locus_tag="VNU01_13015"
     CDS             complement(904..1803)
                     /locus_tag="VNU01_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33584.1"
                     /translation="MNKRLAGALALCLLAVLAATPADARTDDRSKPIVYVHGYDSSGS
                     TIGHSCTGYFDGTYTALESWGHTGERALVRYYRDDTNCNVDSHAHGSHATHYAGGHST
                     GNGHTRDASIRHLGYHLAWTIYDRYTSQGVTVDLIGHSMGGLIARYAIGEVARGNADF
                     PPSLLVEDAVTVGTPHGGSGWAWFCGNTQCVEMRPGSSFLNYLSANSPNPGAPATDWT
                     NIASDDDEVVAWDSATNMAAPHKSIYLTSENLSHSGMLDDNVSARDRDADYMQDNDGV
                     WRFDSAHPRAVLRMDDALVLGTQ"
     gene            complement(1947..2366)
                     /locus_tag="VNU01_13020"
     CDS             complement(1947..2366)
                     /locus_tag="VNU01_13020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH33585.1"
                     /translation="MLATVLSAVLLAAAVLPPVAGPVTRGFDPPPVPYAAGHRGVDLD
                     AAPGEAVRAVLPGVVRFEGTVARVGWVSVDHGGGLVTTYGPLDPRLVAAGGRVRAGDV
                     LGLVADGSRRLDWGARLHGAYLDPLSLLRPWEPFLLP"
     gene            complement(2435..3262)
                     /gene="whiG"
                     /locus_tag="VNU01_13025"
     CDS             complement(2435..3262)
                     /gene="whiG"
                     /locus_tag="VNU01_13025"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HWH33586.1"
                     /translation="MQASPAQGASARGPKAAQGKVSQDPEVVELWERYKADGDARSRE
                     RLILQYSPLVKYVAGRVSVGLPATIEHGDLVSYGMFGLIDAIEKFDLGKGVRFETYAI
                     ARIKGAIIDELRSIDWVPRSVRGKARDVERALQSLEAKLRRTPSEKELAEEMGITLKE
                     LHNTLAQVSLTSLVALDESFSGDDNERQPLVDTIQDHKAPDPEARYEDIEQKQLLAQA
                     IGKMNERERTVVVLYYFEGMTLAQIGEVLGVTESRVCQMHTKAVLGLRTKMTERTLG"
     gene            complement(3344..4273)
                     /locus_tag="VNU01_13030"
     CDS             complement(3344..4273)
                     /locus_tag="VNU01_13030"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HWH33587.1"
                     /translation="MDLPEELPAPWAAALDTLGVHLRDERMLAAHTVAAYLRDARQLA
                     GFCAALGIPDPAEVQPLVLRRFLAALAEEGYARASLARKSAAVRGLFALLARRGAVEA
                     DPALALGTPKGERRLPKVLTVAQVERLLAAPDPSTAAGQRDRALLELLYASGARVSEA
                     VGLDTGAIDLAAGAVRLFGKGSKERLVPLGEPACVALERYLGDGRVRLLASGADADAD
                     AAAVFLNGRGARLGVRDAREAVDRAARLAGLGKVTPHTLRHSYATHLLEGGADLRAVQ
                     ELLGHVALSTTQIYTHVSREHLRSTYEHAHPRA"
     gene            4420..4686
                     /locus_tag="VNU01_13035"
     CDS             4420..4686
                     /locus_tag="VNU01_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33588.1"
                     /translation="MDSALRTPVRDPDAGSRLAAAPPDRPEVIELDPVQSHPVRSKGS
                     KVEQWGLCVSCDRWFYCGTSVEQSRDCRCPACDQAPVAIRETWE"
     gene            complement(4723..>4945)
                     /locus_tag="VNU01_13040"
     CDS             complement(4723..>4945)
                     /locus_tag="VNU01_13040"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33589.1"
                     /translation="PGTANRTRAGGRTAPDGPASAVAAALTAVPASADALRLATGLPV
                     PTVLAALAELTALGLARTTPRGWVGGGRT"
BASE COUNT          643 a         1742 c         1819 g          741 t
ORIGIN      
        1 ggagcgcgag gcgcgcatct ggtagccctc ggcgcaggcg tcggcgatca gccgggtcag
       61 cagcgccccg gagcggatcg cgtcgtcgtt gcccgggatg atgaagtcga tgacgtcggg
      121 gtcgcagttg gtgtcgacga cggcgacgac cgggacgttg aggcggttgg cctccttgac
      181 ggcgatgtgc tccttcaccg tgtcgacgac ccagatggct gacgggggct tggtcatgcc
      241 gcgcaggccg ccgaggttgc gctccagctt ctcgcgctcg cgctggagct ggaggacctc
      301 cttcttgggc agcagttcga actcgccgct gcgctccttc tcctcgagct cgcgcaggcg
      361 ggccaggcgc gtcttgatgg tctcgaagtt cgtgagcatg ccgccgagcc agcggtagtt
      421 gacgtagggc atgccgacgc gggtggcctg cgtctcgatg gcttcctggg cctgcttctt
      481 ggtgccgacg aagaggatgg tgccgccctt ggcgacggtg tcgcgcacgt gctcgtaggc
      541 ctgctccagc atgccgatgg tctgctggag gtcgatgatg tagatgccgt tgcgctcgcc
      601 gtggatgaag cgcttcatct tcgggttcca gcggcgggtc tggtgcccga agtggacgcc
      661 cgcctcgagc aactggcgca tgctgacaac acccatggtg ctaacccctt ttgatttggt
      721 tcggcctcgc gccctcggcc gccagctcga ggctggcctg gccgccggtc cgacgcccgt
      781 catccgcgac gggcgcaccg tggtccgagt cggacgctgt gaatttggcc cgggacacgc
      841 ccgggccgtc gacgaggata ccgccgacgg cccggggtcg cgacttgcgc gcggagtggg
      901 ctgctactgg gtgccgagca cgagcgcgtc gtccatgcgc aggacggcgc gcgggtgggc
      961 ggagtcgaag cgccagacgc cgtcgttgtc ctgcatgtag tcggcgtcgc ggtcacgtgc
     1021 gctgacgttg tcgtcgagca tgccgctgtg gctcaggttc tcgctggtca ggtagatgct
     1081 cttgtgcggg gcggccatgt tggtagccga gtcccaggcg acgacctcgt cgtcgtcgga
     1141 ggcgatgttg gtccagtccg tcgccggtgc gcccgggttg ggcgagttgg cgctcaggta
     1201 gttcaggaag ctcgaccccg ggcgcatctc cacgcactgg gtgttgccgc agaaccaggc
     1261 ccagccgctg ccgccgtgcg gcgtgccgac cgtgaccgcg tcctccacga gcagcgacgg
     1321 cgggaagtcg gcgttgcccc gggcgacctc gccgatggcg tagcgggcga tcaggccgcc
     1381 catcgagtgg ccgatcaggt ccacggtgac gccctgggac gtgtagcggt cgtagatggt
     1441 ccaggccagg tggtagccca ggtggcggat ggacgcgtcg cgggtgtggc cgttgccggt
     1501 ggagtgtcca cccgcgtagt gcgtggcgtg gctgccgtgg gcgtggctgt ccacgttgca
     1561 gttggtgtcg tcgcggtagt agcgcacgag ggcgcgctcc cccgtgtggc cccaggactc
     1621 cagggccgtg taggtgccgt cgaagtagcc ggtgcagctg tggccgatgg tgctgccgga
     1681 cgagtcgtag ccgtgcacgt agacgatggg cttgctgcgg tcgtcggtgc gggcatcggc
     1741 aggcgtcgcc gccaggaccg cgagcaggca cagggcgagt gctcccgcca gtcgcttgtt
     1801 catgtgttcc cccttttcgg actcgtcccg ctcccctgac gggacgtgag ccgagcctct
     1861 cccggggcgg cggcgcgcgt ctattgggca aggcgcgtat gacggcctcc gccgcaggcg
     1921 tgcgcaggcc gcgtacgcgc gggcgcctac ggcagcagga acggctccca tgggcgcagc
     1981 agggacagcg ggtcgaggta ggcgccgtgc aggcgcgcgc cccagtccag cctgcgggag
     2041 ccgtccgcga ccaagcccag gacgtcgccc gcccggacgc ggcctccggc ggccaccagc
     2101 ctggggtcca gcggcccata ggtggtgacc aggccgccgc cgtggtcgac gctgacccag
     2161 ccgacgcggg cgacggtgcc ctcgaagcgc accacgcccg gcagcacggc gcgcaccgcc
     2221 tcgccgggcg ccgcgtccag gtccacgccg cgatggccgg cggcgtacgg cactgggggc
     2281 gggtcgaagc cgcgcgtgac cgggcccgcc accggcggga gcaccgcggc ggcgagcagg
     2341 acggcggaca ggacggtcgc gagcacggcg gcaacgctac gcccaagacg gcccgacagc
     2401 gccggcggag cgcctgtggt tgtggaaagc cgggtcagcc cagcgtgcgc tcggtcatct
     2461 tggtgcgcag gcccagcacg gccttggtgt gcatctggca cacgcgggac tcggtcacgc
     2521 cgagcacctc gccgatctgc gccagcgtca tgccctcgaa gtagtacagg accaccacgg
     2581 tgcgctcgcg ctcgttcatc ttgccgatgg cctgggcgag cagctgcttc tgctcgatgt
     2641 cctcgtagcg cgcctcgggg tcgggcgcct tgtggtcctg gatggtgtcg accaggggct
     2701 ggcgctcgtt gtcgtcgccg gagaaggact cgtcaagggc gaccaaggag gtgagcgaca
     2761 cctgcgcgag cgtgttgtgc agctccttga gggtgatgcc catctcctcg gcgagctcct
     2821 tctcggacgg cgtccggcgg agcttggcct ccagcgactg cagcgcgcgc tcgacgtcgc
     2881 gggccttgcc gcgcacgctg cgcggcaccc agtcgatgga gcgcagctcg tcgatgatcg
     2941 cgcccttgat gcgcgcgatg gcgtacgtct cgaagcgcac gcccttgccc aggtcgaact
     3001 tctcgatggc gtcgatgagc ccgaacatgc cgtagctgac gaggtcgccg tgctcgatgg
     3061 tggcgggcag accgacggac acgcggccgg cgacgtactt caccagcggc gagtactgca
     3121 ggatcagccg ctcgcgggac cgtgcgtcac cgtcggcctt gtagcgctcc cacagctcga
     3181 cgacctcggg atcctgcgaa accttgccct gcgcggcctt ggggccccgc gcgctggcgc
     3241 cttgcgctgg cgatgcctgc atcaagcggt ccgatcgggc cggccgacca ggccctgtcg
     3301 cggcgtgcgg acgccctggc gaagcccctt gccggagccg cagtcaagct ctgggatgtg
     3361 cgtgctcata cgtgctccgc aagtgctccc gcgagacgtg agtgtagatt tgggtcgtcg
     3421 acagggcaac gtgccccagc agttcctgga cggccctcaa gtcggccccg ccctccaaca
     3481 gatgcgtagc gtagctgtgc cggagcgtgt gcggggtcac cttgcccaag cccgccagcc
     3541 tggccgcccg gtccaccgcc tcgcgcgcgt cgcgcacgcc gagccgcgca ccgcgcccgt
     3601 tcaggaacac ggccgccgca tcggcgtccg cgtccgcgcc ggacgccagc aggcgcacgc
     3661 gcccgtcgcc caggtagcgc tccagcgcca cgcaggcggg ctcccccagg ggtaccagcc
     3721 gctccttgga gcctttcccg aacagccgta cggcgccggc ggccaggtcg atggcgccgg
     3781 tgtccaggcc gaccgcctcg ctgacgcggg cgccgctggc gtacagcagc tcgagcaggg
     3841 cgcgatcgcg ctggcccgcc gccgtcgacg ggtcgggtgc ggcgagcagc cgctccacct
     3901 gcgccacggt gagcaccttg ggcaggcggc gctccccctt gggggtcccc agcgccagcg
     3961 ccgggtcggc ctccaccgcg ccgcggcgcg ccagcagcgc gaacagcccg cgcaccgcgg
     4021 cggacttgcg ggccaggctg gcgcgcgcgt aaccctcctc cgccagggcc gccaggaacc
     4081 gccgcagcac cagcggctgg acctccgcgg ggtcggggat gcccagggcc gcgcagaacc
     4141 cggccagctg gcgggcatcc cgcaggtacg ccgccaccgt gtgggcggca agcatgcgct
     4201 cgtcccgcag gtgcacgccg agcgtgtcca gggcggcggc ccacggcgcc ggcagctcct
     4261 ctggcagatc catgccggtc agggtggcac atcggcccgg ccgtccggcg gaggtcctgg
     4321 aagccacgca ccggggcaaa tcgcacggcg attgcgcgcc gagatgcgtt tgtcgcatgc
     4381 aggccgtaac ccccgggcga gcccgtgcgc tgaccggtca tggactcggc actgcgcacc
     4441 cccgtccgtg accccgacgc gggctcgcgc cttgccgccg cgccaccgga tcggcccgag
     4501 gtgatcgagc tggatccggt ccaatcccat cccgtccgat ccaaggggag caaggtggag
     4561 caatggggtc tgtgcgtctc atgcgaccgc tggttctact gcggcaccag cgtggagcag
     4621 tcccgcgact gccgctgccc ggcctgcgac caggcaccgg tcgccatcag ggagacgtgg
     4681 gagtgagcgg gaacccgcac tggctgcgcc tggccgacga cctcaggttc ggccaccgcc
     4741 gacccacccc cgcggcgtgg tgcgcgcgag ccccagagcc gtcaactcgg ccagcgccgc
     4801 gagcaccgtg ggcaccggaa gaccggtcgc caggcgcaga gcgtcggcgc tcgccggtac
     4861 cgccgtcagc gcggctgcca cggcgctcgc cggcccgtcc ggagccgtcc gaccgcctgc
     4921 ccgggtccgg ttcgccgtgc cgggc
//
LOCUS       DATMKC010000449         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_106804, whole genome shotgun sequence.
ACCESSION   DATMKC010000449 DATMKC010000000
VERSION     DATMKC010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106804"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1390)
                     /locus_tag="VNU01_13045"
     CDS             complement(8..1390)
                     /locus_tag="VNU01_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HWH33590.1"
                     /translation="MRALETDYLVVGAGAMGMAFTDALIDHADVHVTLVDRRHAPGGH
                     WNDAYPFVQLHQASVFYGVASTVLGTGAVQQDGPEAGLQERARRAQIQHYYDDILFRR
                     FVGSGRVTFLGGHDYHTDGSSHLATSRVSGETVRVAVRRRVVDAAYLAPTIPATTPPP
                     FDVADTARVIPVHQLADLADAPSSYVIVGSGKTATDAIVWLLRNGVEPDRIVWVRPRE
                     PWMLNRAVVQPDPVVALNLAADTLAAAADAASPDELFLRLEAAGVMLRIDTGLTPTMA
                     KTPTLGTWELDLLRTVERVVRLGHIRSVSASEIVFQHGVVPLQPGALVVHCAASGLQY
                     PPLVPVWSPDKIRLQTIRAGFPTFCAALAGYVEATRDDDRERNRVCPANSLSNTPADW
                     ALMQVRGTLATRAYGAEPDIAEWANACALNPARIDASRRDDPAVLAAAARLADVAEPG
                     LARLAELAGG"
     gene            complement(1572..1799)
                     /locus_tag="VNU01_13050"
     CDS             complement(1572..1799)
                     /locus_tag="VNU01_13050"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWH33591.1"
                     /translation="MEVGVHEAKTTLSKLLKRVAAGEEITITRGGEPVARLVAAQGAP
                     RRTLGQDRGRYGVPDDFDAPLDEAALRAFEA"
     gene            complement(1828..>3027)
                     /locus_tag="VNU01_13055"
     CDS             complement(1828..>3027)
                     /locus_tag="VNU01_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33592.1"
                     /translation="ALDSGKAGWAMGKRARRQRREGGGHGPAAGIAGASALWNEPDFL
                     AAIRRALRQDHPWNLLSLVSVMLEIVDPRSISEALDEAPRTFAHRDELVELLISSRRL
                     ESTAMLAAISEVAGEPVAGRIHAELAARDDELPGWLTALGPLTIDGVFETDYVLGDGN
                     HVLIGAQTAAGHPFTCMVYIDHNFGTVVKDGGAIWAPIESVAAALRQEFNSESDCGMR
                     PLDPATARVRITQAIEAATRIVPPLHRPTWPAARPVAEWVVRHLPDGGAAPVRTEWGR
                     SSREGLMNAFLSSSFGEAFEAEARDALDQVLAFACDFGAGDPLRWSPARIAVLLTRWL
                     PMRLLPNGPSFRLLPDVLRAFVRYSHAEQGVRADLTADTLAMVDECEPDFRDAVISGH
                     ARLEPVD"
BASE COUNT          453 a         1068 c         1074 g          432 t
ORIGIN      
        1 gcgcgcgtca gccgccggcg agctcggcca accgggccag ccccggctcg gcgacgtcgg
       61 ccaggcgcgc ggcggcggcg agcaccgccg ggtcgtcgcg tcgcgacgcg tcgatgcgcg
      121 ccgggttgag cgcacaggcg ttggcccact cggcgatgtc gggctcggcg ccgtacgccc
      181 gggtcgccaa ggttccgcgc acctgcatga gcgcccagtc ggccggggtg ttggacaggc
      241 tgttggccgg gcacacccgg ttgcgctccc gatcgtcgtc ccgggtcgct tccacgtagc
      301 cggccagcgc ggcgcagaac gtcgggaagc cggcccggat ggtctgcagc cggatcttgt
      361 ccgggctcca caccggcacc agcggcgggt actgcagccc ggacgcggcg cagtgcacca
      421 ccagggcgcc cggctgcagg ggcacgacgc cgtgctggaa cacgatctcg cttgcggaca
      481 cggacctgat gtgacccagg cgcacgacgc gctcgacggt gcggagcaga tccagctccc
      541 aggtgccgag cgtcggcgtc ttcgccatgg tgggcgtgag gccggtgtcg atgcgcaaca
      601 tgaccccggc ggcttccagg cggaggaaca gctcgtcggg tgaggcggcg tcggccgcgg
      661 cggccagcgt gtcggcggcg aggttcagcg caaccacggg gtcgggctgg acgacggcgc
      721 ggttgagcat ccacggctcg cgcgggcgga cccacacgat gcggtccggc tccacgccgt
      781 tgcgcagcag ccagacgatg gcgtcggtcg cggtcttgcc ggacccgacg atcacgtagc
      841 tgctgggcgc gtcggccagg tccgcgagct ggtgcacggg gatcacgcga gccgtgtcgg
      901 cgacgtcgaa cggcggcggg gtggtggccg ggatcgtcgg tgccaggtac gccgcgtcga
      961 ccacgcggcg gcgcacggcg acgcgcaccg tctcccccga cacgcgcgac gtggccaggt
     1021 gggagctgcc gtcggtgtgg tagtcgtgcc cgcccaagaa ggtcacccgg cccgagccga
     1081 cgaagcggcg gaacaggatg tcgtcgtagt agtgctggat ctgcgcgcgg cgcgcgcgct
     1141 cctgcaagcc ggcctcgggg ccgtcctgct gcacggcgcc ggtgcccagg acggtggacg
     1201 cgacgccgta gaacaccgac gcctggtgga gctggacgaa cgggtaggcg tcgttccagt
     1261 ggccgccggg cgcgtgacgg cggtcgacca gcgtgacgtg cacgtccgcg tggtcgatca
     1321 aggcgtcggt gaacgccatt cccatcgcgc ccgcgccgac gaccaggtag tcggtttcca
     1381 gtgcccgcat tgccacgctg cccaccccct gaaggcgacg aacgagtcgg ccgcacagac
     1441 cctacggcac cacggcgcgt ttggtcagcc ggccgcgatc agttccacgc cgtaggcgcg
     1501 caactgcggg tccgcggtga tgatcggcac gccttccagg cgggcctgcg cgatgagcaa
     1561 gcggtcgaag atcacgcctc gaatgcgcgg agagcggcct cgtcgagcgg ggcgtcgaag
     1621 tcgtccggta ccccgtagcg cccacggtcc tgaccgagcg tccgccgtgg cgcgccttgt
     1681 gcggcgacca gccgggcgac cggctcgccg ccgcgggtaa tggtgatctc ctcgcccgcg
     1741 gccacgcgct tcagcagctt cgacagcgtg gtcttcgcct cgtgcacacc tacttccatg
     1801 gcccccaagg taggcttggc tagtcactca gtccaccggt tcgagtcgcg cgtgcccgga
     1861 gatcacggcg tcgcggaagt cgggctcgca ctcgtcgacc atggcgagcg tgtccgccgt
     1921 caggtccgcc cggacgccct gctccgcgtg gctgtaccgc acgaatgctc gcagcacatc
     1981 gggcaggagc cggaaggagg gcccgttcgg cagaaggcgc atgggcagcc atcgggtcag
     2041 cagcaccgcg atacgcgcgg gactccaacg cagcgggtct ccggcaccaa agtcacatgc
     2101 aaacgccagc acctgatcca gcgcatcacg agcctccgcc tcgaacgcct cgccgaacga
     2161 agaggacagg aaggcgttca tcagaccctc ccgggaggac cggccccatt cggtccgcac
     2221 gggagcggcg cccccgtcag gcaggtggcg caccacccat tccgctaccg gtctggcggc
     2281 cggccacgta ggcctgtgca gaggtggaac gatccgcgtc gccgcttcga tggcctgggt
     2341 gatccgcacc cgcgccgtcg ccgggtcgag gggccgcatg ccacagtccg attccgagtt
     2401 gaattcctgg cgcagtgccg ccgccacgct ctcgatcggt gcccagatgg ccccgccatc
     2461 cttgaccacc gtgccaaagt tgtggtcgat gtagaccatg caggtgaacg ggtgtcccgc
     2521 tgccgtctgc gctccgatga gcacatggtt accgtcgccg agcacgtagt ccgtctcgaa
     2581 tacgccgtcg atggtcaacg gtccaagggc ggtgagccag cccggaagct cgtcgtcgcg
     2641 ggctgccagc tcagcatgga tacggccggc gaccggctct ccggccacct cggagatcgc
     2701 cgcaagcatg gcggtcgact ccagacggcg tgaggagatc agcagctcca ccaactcgtc
     2761 gcggtgagcg aacgtacgag gagcctcgtc gagggcctct gagatgctcc gcgggtcgac
     2821 gatctcgagc atgacggata cgaggctgag caagttccac ggatgatcct gccgcagtgc
     2881 tcgtcgaatg gccgcgagga aatccggctc gttccagagc gccgatgctc ctgcaatccc
     2941 ggccgcgggc ccgtgaccgc caccctctcg cctctgcctg cgcgcacgct tgcccatcgc
     3001 ccatcccgcc tttccgctgt cgagtgc
//
LOCUS       DATMKC010000450         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_235785, whole genome shotgun sequence.
ACCESSION   DATMKC010000450 DATMKC010000000
VERSION     DATMKC010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235785"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VNU01_13060"
     CDS             <1..701
                     /locus_tag="VNU01_13060"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWH33593.1"
                     /translation="LERRDGVRYPVLVPNERGLARALESGVDEVAIFGSATETFARRN
                     LNRTVAESIEMFRPVAERAQQAGARVRGYLSMCFGDPWEGDVPVAQVVDVGTRLLELG
                     CYELSLGDTIGVGTPGHVRALLDAFGERGVGAERLAVHFHDTYGMALANTVEALRWGV
                     SVVDASAGGLGGCPYAKSATGNLATEDLVWLLDGLGVDTGVDLGKLVETSRWMAEQLG
                     RPAPSRVVQALSSG"
     gene            811..2355
                     /locus_tag="VNU01_13065"
     CDS             811..2355
                     /locus_tag="VNU01_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWH33594.1"
                     /translation="MAGTRAAAGAGALAAGAALGAGELLAGFGESVPSLVRAVGAGAV
                     DLAPPGLKDFAVAAFGTADKPALLIGITVVSLLLGAGLGLLARGARGLAAGGFMAFGA
                     LGLVAGATAPTGTLPGALAAALAATAAGVVALLLLMRRWDAAYGSRHAGPAPGGADAA
                     RRDFFRVAGATGALALTGGAAGRWLSRAGEQVEAQRAAVALPAPASPAPPPGQGLSVR
                     GLTPLYVPNARFYRIDTALTVPRVDIDGWRLRVGGPPGGKAREYRYDELLAMDLAEAD
                     VTLACVSNEVGDDLVGNARWLGVPLAALLTGALEERPPMPGTQVVGRSVDGFTAAFPL
                     EALTPERTALVALGMNGEPLPARHGFPARLVVAGLYGYVSATKWLSSIELHGPEFDGY
                     WVPRGWAKEAPVKTQSRIDVPRHGAEVAAGRVAVAGVAWAPHRGVEAVEVRVGDGEWR
                     AARLSESIGADAWRQWVLAWDAEPGLHRLQVRATDGLGRTQTERRTPARPDGATGHHT
                     VDVRVI"
     gene            2443..2976
                     /locus_tag="VNU01_13070"
     CDS             2443..2976
                     /locus_tag="VNU01_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWH33595.1"
                     /translation="MPLFAFEGKAPEVHATAFVAPTATLVGEVVVEEGASVWYGAVIR
                     ADYAPVYVRKGANVQDGAVLHAPPNHPTEIGAGATVAHNCVVHGATVGEEALVANGAI
                     VLDGATVGRQALVAAGAIVTAGSSIGERMLAMGAPAKEKGPIDGTPAEFWVSLNPAGY
                     QDLARRHREGVTPIPEG"
     gene            3119..3412
                     /locus_tag="VNU01_13075"
     CDS             3119..3412
                     /locus_tag="VNU01_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWH33596.1"
                     /translation="MTQLTLEDGDVRAVLRAALSTGGEFAEIYAEDRRARNLRLDDGR
                     VEDVVSGVDRGAGVRVVAGRRTAYAYTNVLTRDALVEAAKAASTRASRVSTLV"
BASE COUNT          398 a         1239 c         1342 g          441 t
ORIGIN      
        1 cgctggagcg ccgcgacggc gtgcgctacc ccgtgctggt ccccaacgag cgcggcctgg
       61 cgcgggcgct ggagtccggc gtcgacgagg tcgcgatctt cggcagcgcc acggagacgt
      121 tcgcgcggcg caacctcaac cgcaccgtgg ccgagtccat cgagatgttc cggcccgtgg
      181 ccgagcgggc gcagcaggcg ggcgccaggg tgcgcggcta cctgtcgatg tgcttcggcg
      241 acccgtggga gggcgacgtg ccggtcgccc aggtggtcga cgtcggcacg cggctgctgg
      301 agctgggctg ctacgagctg tccctgggcg acaccatcgg ggtgggcacg cccgggcacg
      361 tgcgggcgct gctggacgcg ttcggcgagc ggggcgtcgg cgccgagcgg ctggccgtgc
      421 acttccacga cacctacggc atggcgctgg ccaacaccgt ggaggcgctg cgctggggcg
      481 tgagcgtggt cgacgcgtcc gcgggcgggc tgggcggctg cccgtacgcg aagtcggcga
      541 cgggcaacct ggccaccgag gacctggtgt ggctgctgga cggcctcggt gtcgacacgg
      601 gggtggacct gggcaagctg gtggagacga gccgctggat ggccgagcaa ctgggccgcc
      661 cggcaccatc gcgagtcgtc caggcactgt ccagcggctg acccacccac ccatccaccc
      721 atccaccatc tatccgttca cagtgggcgg tttccccgaa ataagggggt aacggttcac
      781 cgtgcgggcc gaagacggcg aggaggagcc atggcgggga cgcgagcggc ggcaggcgct
      841 ggtgcgctgg cggccggcgc cgcgctcggc gccggggagc tgctcgccgg cttcggtgag
      901 tcggtgccgt cgctggtgcg cgccgtcggc gccggcgcgg tcgacctcgc cccgcccggt
      961 ctgaaggact tcgcggtggc ggcgttcggc accgccgaca agcccgcgct gctgatcggc
     1021 atcacggtgg tgagcctgct cctcggcgcg gggctcgggc tgcttgcccg cggcgcccgc
     1081 ggcctggcgg ccgggggctt catggcgttc ggcgcgctgg ggctggtggc cggcgccacg
     1141 gcgcccaccg gcacgctgcc gggcgcgctg gccgccgcgc tcgccgccac cgcggcaggc
     1201 gtcgtcgccc tgctgctgct gatgcgccgc tgggacgccg cgtacggctc tcgccacgcg
     1261 ggccccgcgc cgggcggcgc cgacgcggcg cgccgtgact tcttccgcgt cgccggtgcg
     1321 accggcgcgc tggccctcac cgggggtgcc gcgggacgct ggctgtcccg cgcgggggag
     1381 caggtggaag cccagcgcgc cgccgtggcg ctgccggcac ccgcgtcgcc cgcgccgccg
     1441 cccgggcagg gcctgtccgt gcgcgggctc acgccgctgt acgtgcccaa cgcgcgcttc
     1501 taccgcatcg acaccgcgct gaccgtgccg cgcgtcgaca tcgacggctg gcgcctgcgc
     1561 gtcggcgggc cgccgggcgg caaggcgcgc gagtaccgct acgacgagct gctcgcgatg
     1621 gatctcgccg aggccgacgt cacgctggcg tgcgtgtcca acgaggtggg tgacgacctg
     1681 gtcggcaacg cccgctggct cggcgtgccg ctggcggcgc tgctgacggg tgcgctcgag
     1741 gagaggccgc caatgccggg cacgcaggtg gtgggccgct cggtcgacgg cttcacggcg
     1801 gccttcccgc tggaggcgct cacgcccgag cgcaccgcgc tggtcgcgct cggcatgaac
     1861 ggcgagccgc tgcccgcacg gcacggcttc ccggccaggc tggtggtcgc cgggctgtac
     1921 ggctacgtgt ccgccacgaa gtggctgtcg tccatcgagc tgcacgggcc ggagttcgac
     1981 ggctactggg tgccgcgcgg ctgggccaag gaggcgccgg tgaagacgca gtcgcgcatc
     2041 gacgtgccgc gccacggcgc cgaggtcgcc gccggacggg tggccgtcgc cggcgtcgcg
     2101 tgggcgcccc accgcggcgt ggaggccgtg gaggtgcgcg tcggcgacgg cgagtggcgg
     2161 gcggcgcggc tgtcggagtc catcggcgcg gacgcctggc ggcagtgggt gctggcctgg
     2221 gacgccgagc cgggactgca ccgcctgcag gtccgcgcga ccgacgggct cggccgcacg
     2281 cagaccgagc ggcgcacccc ggcgcgcccg gacggcgcca ccggccacca caccgtcgac
     2341 gtccgcgtga tctagcctgc ccgtccacag tgggcggttc ccctcatata cgggggtaac
     2401 cgcccactgt gggcggaaac tgcgggacga gggaggcacg acatgccgct gttcgcgttc
     2461 gagggcaagg cgcccgaggt gcacgccacc gccttcgtgg cgccgaccgc cacgctggtc
     2521 ggcgaggtgg tcgtcgagga gggcgcctcg gtctggtacg gcgccgtcat ccgcgccgac
     2581 tacgcgccgg tgtacgtccg caagggtgcc aacgtgcagg acggcgccgt gctgcacgcg
     2641 ccgcccaacc accccaccga gatcggcgcc ggcgccaccg tcgcgcacaa ctgcgtcgtg
     2701 cacggggcca ccgtcggcga ggaggcgctc gtcgccaacg gggcgatcgt gctggacggc
     2761 gccacggtcg ggcgccaggc gctggtcgcc gccggggcga tcgtgaccgc gggcagcagc
     2821 atcggcgagc gcatgctcgc catgggcgcg cccgccaagg agaagggccc catcgacggc
     2881 acgccggccg agttctgggt gtccctcaac cccgccggct accaggacct ggcgcgacgc
     2941 caccgcgagg gagtgacgcc gatccccgag gggtaggccc gcctccacct gccccacgga
     3001 ggaggagacc tcatgctgag ctggatcctg gcggtgccgt tctggaccgg tgtcgtcgtg
     3061 ggcgtgctgc tgacgctgct ggtccaggcc ttgttctgac gtcctctacg ctgcccgcat
     3121 gacccagctg accctcgagg acggtgacgt ccgcgcggtc ctgcgggccg ccctgtccac
     3181 cggcggcgaa ttcgccgaga tctacgccga ggaccgccgc gcccgcaacc tgcgcctgga
     3241 cgacgggcgc gtggaggacg tggtgtccgg cgtcgaccgc ggcgcgggcg tgcgcgtggt
     3301 cgccggccgg cgcaccgcct acgcctacac caacgtgctc acccgcgacg ccctggtgga
     3361 ggccgccaag gcggcctcca ccagggcgtc gcgggtgagc acgttggtgt aggcgtaggc
//
LOCUS       DATMKC010000451         5232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_129379, whole genome shotgun sequence.
ACCESSION   DATMKC010000451 DATMKC010000000
VERSION     DATMKC010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129379"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1048
                     /gene="add"
                     /locus_tag="VNU01_13080"
     CDS             44..1048
                     /gene="add"
                     /locus_tag="VNU01_13080"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWH33597.1"
                     /translation="MRTIDEYRAMPKAELHIHLDGSVRPATIDELAREVGLVPHDAPP
                     GFAAERVLVTERAGLLEALAGFDILLPILQTPQRLRRVTAELVEDLAAEGVTYAELRF
                     APRLFTDEGLTSDAVLGHTAEAAAEAAEATGTKAPLIACLMGGRDPEWNADIVDAALD
                     GRGDGVVAVDLAGPVDGRTPEWVDAHAYLFAKARDAGLRVTIHAGEAEPPDAIRTALE
                     RYGAERIGHGTTLVADEALLAEVAERGIPVESCPRSNYWTRTAAEMPRLSDHPVGAVL
                     RAGAVVCLNTDDRSLFANDLSSELAVTQEALELTDAEVDRLIANGWAAAFAAPTRPSR
                     "
     gene            complement(1115..1264)
                     /locus_tag="VNU01_13085"
     CDS             complement(1115..1264)
                     /locus_tag="VNU01_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33598.1"
                     /translation="MTDQAFVAMPSCGNQAGELCPNCWEAVLREEGAHGRCDACGWIG
                     KCCEG"
     gene            complement(1396..2145)
                     /locus_tag="VNU01_13090"
     CDS             complement(1396..2145)
                     /locus_tag="VNU01_13090"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HWH33599.1"
                     /translation="MYTNPFTTARRGTRAVLRAGVVAAASTVALSGVAAPAAAHEGLP
                     TQAPTQTRDLPAAAKPQNGPAESTIHIVARGESLSSIAASYGYTGEDDWRHIYDANPT
                     KLDDPNLIFTGLHLRIPTAEENFVRRDLPVPVVAAVEAAPRRAGAARASRSATPAPSG
                     GAAPAVAGAGVWDQLAQCESGGNWSINTGNGYSGGLQFHPGTWTGHGGGEYAPTASQA
                     TREQQIAVAQRVQASQGWGAWPACSRRLGLR"
     gene            complement(2479..2802)
                     /locus_tag="VNU01_13095"
     CDS             complement(2479..2802)
                     /locus_tag="VNU01_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33600.1"
                     /translation="MSRLRTTALLAAAALSLTACGDAVSSMTEHWFYGYVVSVDAAEL
                     CLDDARTEDLQAQRCFDATEAGGVEELAEGDLVKVRYEREDGGHGGGRAVEARMVRAA
                     GRSDG"
     gene            complement(2824..4044)
                     /locus_tag="VNU01_13100"
     CDS             complement(2824..4044)
                     /locus_tag="VNU01_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH33601.1"
                     /translation="MDFGFTDRMRELHGQVREFMDSVVIPNEPVYHRQVAESGDRYFH
                     PPVMEEMKAQARAAGLWNLFLPDSEWGAGLSNLEYAPLAELMGRSHIGSEVFNCSAPD
                     TGNMEVLARYGTPEQQQEWLVPLLNGEIRSAFSMTEPDVASSDASNIQSRIERDGDEY
                     VINGRKWWTSGAGRDRCKISIFMGKTDPSAPPYQQQSMILVPMETPGVEVVRGLSVFG
                     YDGGEGHMEMTFTDVRVPASNLLQNEGDGFRIAQGRLGPGRIHHCMRTIGMAERALEL
                     MCRRVASRRAFGKELARQGVIQEWIAESRCDIEQARLLTMKAAWMMDTVGNKAAKAEI
                     AMIKIVAPTMALKVIDRAIQAHGGMGVSQDTPLAAAWAGIRTLRLADGPDEVHKMSLA
                     KQELRRQLDGEIDL"
     gene            complement(4124..5197)
                     /locus_tag="VNU01_13105"
     CDS             complement(4124..5197)
                     /locus_tag="VNU01_13105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWH33602.1"
                     /translation="MSGHGEGTTDAGGVRSFASAFFEALTRPLPRHIADFRESARTAL
                     IFAALVIVLGTLWIPQAGAVRIPGVLLLSLITAATGVVSMMPAAHAVMNRYGGVLTLL
                     QIVGIVWLTGGPDSIYSMLYVMLLLYAALFYDTMRLIATGVLILVAIVAPYFYGGRPP
                     HYLAELLVTVPVWAGITGTVHWLVHRIRTSAQTDGLTGLWNHVTFWRLAQGEHERMLR
                     HGSHYSVLMLDLDHFKKINDTRGHSAGDDVLRQIAEVLTSRSRASDIVARYGGEEFAM
                     LLPETHREQAVELAKELRMRVLTTTMQVPVTVSIGVASSADGLTRSAESVVAAADRAL
                     YEAKRAGRNHVAVSLPGQEASPV"
BASE COUNT          706 a         1877 c         1917 g          732 t
ORIGIN      
        1 atgagcagca cgcgcgccat gcggccagcg taacctcgca cgcatgcgca ccatcgacga
       61 gtaccgcgcc atgcccaagg ccgagctgca catccacctc gatggctcgg tccgccccgc
      121 caccatcgac gagctcgccc gcgaggtggg gctggtgccg cacgacgcgc cgcccggctt
      181 cgcggccgag cgcgtgctgg tgaccgagcg cgccgggctg ctggaggcgc tggcgggatt
      241 cgacatcctc ctgccgatcc tgcagacccc gcagcgcctg cggcgcgtga ccgccgagct
      301 ggtggaggac ctcgccgccg agggcgtcac ctatgccgag ctgcgcttcg cgcccaggct
      361 gttcaccgac gaggggctga ccagcgacgc ggtgctcggc cacaccgcgg aggcggccgc
      421 ggaggcggcc gaggccacgg gcaccaaggc gccgctgatc gcctgcctca tgggcggcag
      481 agacccggag tggaacgccg acatcgtgga cgcggcgctt gatggccgcg gcgacggggt
      541 cgtggccgtg gacctggcgg ggccggtcga cgggcgcacg cccgagtggg tcgacgcgca
      601 cgcgtacctg ttcgcgaagg cgcgcgacgc ggggctgcgg gtgaccatcc acgccggcga
      661 ggccgagccc ccggacgcga tccgcacggc gctggagcgc tacggcgcgg agcgcatcgg
      721 ccacggcacc acgctggtgg ccgacgaggc gctgctggcg gaggtggcgg agcgcggcat
      781 ccccgtggag agctgcccgc gctccaacta ctggacgcgc acggcggcgg agatgccgcg
      841 gctgtccgac catccggtgg gcgcggtgct gcgcgcgggc gccgtggtgt gcctcaacac
      901 cgacgaccgc agcctgttcg cgaacgacct cagctccgag ctggcggtca cgcaggaggc
      961 gctggagctc accgacgccg aagtggaccg gctgatcgcc aacggctggg ccgcggcctt
     1021 cgccgccccc acgcgtccgt cccgctgacc gcagctcccg cccactgtgg gccgcttccc
     1081 tgtgatacgg ggggaacggt tcacgacgcc ggggtcagcc ttcgcagcac ttgccgatcc
     1141 agccgcaggc gtcgcagcgc ccgtgggcgc cctcctcccg caggaccgcc tcccagcagt
     1201 tcgggcacag ctcccccgcc tgattcccgc acgacggcat cgcgacgaaa gcctgatcgg
     1261 tcatgcgccg aagagtacgg cgaaagaacg ccaagaaacg ggaaggaggg cccggtcggg
     1321 acccgcgaag gaggcgtaaa gggttcttgc ctccgcagcg ggtcccgaac cggaccctcc
     1381 ttccggtgcg cggaactagc gcaggccgag cctgcgagag caggccggcc aggcgcccca
     1441 gccctgcgag gcctggacgc gctgcgccac tgcgatctgc tgctcccggg tggcctggct
     1501 cgccgtcggc gcgtactcgc cgccgccatg gccggtccag gtccccgggt ggaactgcag
     1561 gccgccggag tagccgttgc cggtgttgat gctccagttg ccaccggact cgcactgcgc
     1621 gagctggtcc cagacgccgg cgccggcgac cgccggagcg gcgccgccgg acggcgcggg
     1681 cgtcgcggag cggctggcgc gcgcggcgcc ggctcggcgc ggcgcggcct ccaccgcggc
     1741 cacgacgggc accggcaggt cacggcgcac gaagttctcc tccgcggtcg ggatgcgcag
     1801 gtgcagcccg gtgaagatca ggttggggtc gtcgagcttg gtggggttgg cgtcgtagat
     1861 gtggcgccag tcgtcctcgc cggtgtagcc gtagctcgcg gcgatggagg acagcgactc
     1921 gccccgggcg acgatgtgga tggtcgactc ggcggggccg ttctgcggct tggcggccgc
     1981 gggcaggtcc cgggtctgcg tcggggcctg cgtcggcagt ccctcgtggg cggccgcggg
     2041 cgcggccacc ccgctcagcg ccaccgtcga ggcggcggcg accacgccgg cacgcaggac
     2101 ggcgcgcgtg ccgcggcggg cggtggtgaa ggggttggtg tacatctgac ctcgctgtga
     2161 cgcgggttgt gtgcggacgc gcgcacgtcc agcccggtat gggcggcaaa gccgtgcggt
     2221 cgtcgtccgg gcccgttcct gtctcgggcc tcggcccccc ggccggcgcg tgtaatcgcg
     2281 ccgttgtcgt ccctgaaggg acaggccggg cggccgtagc gggacgcggg ggacgcgtcc
     2341 gagaaccgac cttagccacc cgcaagtggc cggctcctgt ccggacgggc acggcgcggc
     2401 gtcggagggg acccgggccg ccgcggcgag gcgcgccgga cagggtcggg cgccgcgcgc
     2461 ccagggggtt tccggcggtt acccgtccga acgtccagcg gcgcgaacca ttcgggcctc
     2521 aacggcccgc ccgccaccgt gcccgccgtc ctcgcgctcg tagcgcacct tcacgaggtc
     2581 gccttcggcc agctcctcca cgccgccggc ctcggtggcg tcgaagcagc gctgcgcctg
     2641 gaggtcctcg gtgcgggcgt cgtcgaggca cagctccgcc gcgtcgacgg acaccacgta
     2701 gccgtagaac cagtgctcgg tcatcgacga cacggcgtcg ccgcacgccg tcagggacag
     2761 tgcggcggcg gcgagcaggg ccgtggtgcg caggcggctc atcggtgcga cggttccttt
     2821 ccgctacagg tcgatctcgc cgtcgagctg acggcggagc tcctgcttgg cgagactcat
     2881 cttgtggacc tcgtccggcc cgtcggccag gcgcagcgtg cggatgccgg cccacgcggc
     2941 cgccagcggg gtgtcctggc tgacgcccat cccgccgtgg gcctggatcg cgcggtcgat
     3001 gaccttgagc gccatggtgg gggccacgat cttgatcatc gcgatctcgg ccttcgcggc
     3061 cttgttgccc accgtgtcca tcatccaggc ggccttcatg gtgagcagcc gcgcctgctc
     3121 gatgtcgcag cgcgactcgg cgatccactc ctgtatcacg ccctggcggg cgagctcctt
     3181 gccgaaggcg cggcggctcg cgacgcgcct gcacatgagc tccagggcgc gctcggccat
     3241 gccgatggtg cgcatgcagt ggtggatgcg cccgggcccc agccggccct gcgcgatgcg
     3301 gaagccgtcg ccctcgttct gcagcaggtt cgacgccggc acgcgcacgt ccgtgaaggt
     3361 catctccatg tggccttcgc cgccgtcgta gccgaacacc gacagcccgc gcaccacctc
     3421 gacgcccggc gtctccatgg gcaccaggat catcgactgc tgctggtagg gcggcgcgga
     3481 cgggtcggtc ttgcccatga agatggagat cttgcagcgg tcccggcccg cgccgctggt
     3541 ccaccacttg cgcccgttga tgacgtactc gtcgccgtcg cgctcgatgc ggctttggat
     3601 gttggaggcg tcggagctcg cgacgtccgg ttcggtcatg gagaacgccg agcggatctc
     3661 gccgttgagc agcgggacca gccactcctg ctgctgctcg ggtgtgccgt agcgcgccag
     3721 cacctccatg ttcccggtgt cgggcgccga gcagttgaag acctcggagc cgatgtggct
     3781 gcggcccatg agctcggcca gcggcgcgta ctccaggttg gacaggccgg cgccccactc
     3841 gctgtccggc aggaacaggt tccacaggcc cgcggccctg gcctgcgcct tcatctcctc
     3901 catcaccgga gggtggaagt agcggtcccc cgactccgcc acctgccggt ggtagaccgg
     3961 ctcgttgggg atgaccacgg agtccatgaa ctcacggacc tgcccgtgca gctcccgcat
     4021 gcggtcggtg aaaccgaagt ccacgcctgc tcccctgctc gcgcccgatt cgacggacgc
     4081 atgctagcgg acccgccggc tgcgccggcg gccggcgggg gccctagacg ggggacgcct
     4141 cctggcccgg cagggacacg gcgacgtggt tgcgcccggc gcgcttggcc tcgtacagcg
     4201 cccggtcggc ggccgcgacc acggactcgg cgctgcgcgt caggccgtcg gcgctggagg
     4261 ccacgccgat ggacacggtg acggggacct gcatggtcgt ggtgagcacg cgcatgcgca
     4321 gctccttggc cagctccacc gcctgctcgc gatgggtctc cggcagcagc atcgcgaact
     4381 cctcgccgcc gtagcgggcg acgatgtcgc tcgcgcggct gcggctggtg agcacctcgg
     4441 cgatctggcg cagcacgtcg tcaccggcgg agtgcccgcg cgtgtcgttg atcttcttga
     4501 agtggtccag gtccagcatc agcaccgagt agtgcgagcc gtgccgcagc atgcgctcgt
     4561 gctcgccctg cgcgagccgc cagaaggtca cgtggttcca caggccggtc aggccgtcgg
     4621 tctgcgcgga cgtgcggatg cggtgcacca gccagtggac ggtgccggtg atgcccgccc
     4681 acaccggcac ggtcaccagc agctcggcca ggtagtgggg cggccggccg ccgtagaagt
     4741 acggcgccac gatggccacc aggatcagca cgccggtggc gatcagccgc atggtgtcgt
     4801 agaacagcgc cgcgtacagc agcagcatca cgtagagcat cgagtagatg ctgtcggggc
     4861 cgccggtgag ccagacgatg ccgacgatct gcagcagcgt cagcacgccg ccgtagcggt
     4921 tcatcacggc gtgcgcggcc ggcatcatgc tgaccacgcc ggtcgccgcg gtgatcaggg
     4981 acagcaggag cacaccgggg atccgaaccg cgcccgcctg cgggatccac agggtgccca
     5041 ggacgatcac gagcgcggcg aagatgagcg cggtgcgcgc cgactctcgg aagtcggcga
     5101 tgtgccgcgg cagcgggcgc gtcagcgcct cgaagaacgc ggacgcgaag ctgcgaaccc
     5161 cgcctgcgtc ggtggtgcct tcaccgtgtc cactcatgaa accccgtccg ctccccgcga
     5221 ggttctctat ac
//
LOCUS       DATMKC010000452         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_67840, whole genome shotgun sequence.
ACCESSION   DATMKC010000452 DATMKC010000000
VERSION     DATMKC010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67840"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..355
                     /locus_tag="VNU01_13110"
     tRNA            269..355
                     /locus_tag="VNU01_13110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:303..305,aa:Leu,seq:gag)
     gene            complement(398..1327)
                     /locus_tag="VNU01_13115"
     CDS             complement(398..1327)
                     /locus_tag="VNU01_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33603.1"
                     /translation="MDVVAAMTSDARGPLLRPAVRLVLIAALLAAGGLAWGLANREGA
                     APGLPPADDPGPVHVHGLGLNPADERVYVATHTGLWRLASDGVPERVGDTYADFMGFS
                     VVGPDHFLASGHPDLRTDLPPLLGLIESRDGGVTWQSVSRLGVADFHALRAAHDRVYG
                     WSSTDGTFSVSADGTEWERRATVSVIDFVVDPADPDRVLASSAESLDAAALVASGDGG
                     RTWGPVEGPGVERLSWEAPDRLFGVDARGRVWRSRDGGGSWEQAGAVEGRAGALLDTG
                     DALYAAAGSAIVRSTDDGATWTVLFRPDAEEHP"
     gene            complement(1327..>2379)
                     /locus_tag="VNU01_13120"
     CDS             complement(1327..>2379)
                     /locus_tag="VNU01_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33604.1"
                     /translation="VLGQAFLALAEDGAGELMGPRGAWWLDRLEGELDNFRAALDWMA
                     ARDPACALRLGAALWRVWQMRGHLHEGRTRLEALIAASADADAALRARGFEALGGLAY
                     WQADMPAARAAYERVLDLERELGDGERVAEALYNLAFTYQLGVEDVQASDIGRTMSLL
                     GQALELYREAHADLGAAKCLWAMSTNTYDGAVARLERGEADEAREDFVRALGYGTESL
                     EVFRRHGDWFGTGWALHAVGMSLVRLDRLAEAREAFIEGLDVFVEHRDISGVMLLLDD
                     IAARAARAGQGDDALRIRGGAERLRHDTGTDLARLVNAHDGRFEAADLDERQGALWRE
                     GAALSMEELIALARSL"
BASE COUNT          310 a          976 c          817 g          276 t
ORIGIN      
        1 gcggagcagc gggccgacga ggcgaccgcc gtggccgacc aggacgtgga gccggaggcc
       61 gaggccgtgg tgcacggccc cgagggacag ggcggcgcgg ccggcgcggc ggcccccaac
      121 ggcggcgtca cgggcggcga cccggacgct ccccgcgagt agcaagccgg actttccggg
      181 cccctctgtc acgcgccccc gcggcgctat ccttgcccgg cggagctttg cttgtgccct
      241 cgggccgctg gcatactccc gcagcctcgc gagtgtggcg gaacaggcag acgcgcatgg
      301 ttgagggcca tgtggacttc ggtccttggg ggttcaagtc cccccactcg caccagccca
      361 cctcctgacc cgggcggcgt cagcccaggg tcggacgcta cgggtgctcc tcggcgtccg
      421 ggcggaacag caccgtccac gtcgcgccgt cgtccgtcga gcgcacgatc gcggagccgg
      481 cggccgcgta cagcgcgtcg ccggtgtcca gcagcgcccc ggcgcgcccc tccaccgcac
      541 cggcctgctc ccagctcccg ccgccgtccc ggctgcgcca cacgcgcccc cgcgcgtcga
      601 cgccgaacag gcggtcaggc gcctcccacg acaaccgctc caccccgggc ccctcgacag
      661 gaccccaggt gcggccgccg tcgccgcttg cgacgagcgc ggccgcgtcg agcgactcgg
      721 cgctgctcgc cagcacccga tcggggtcgg ccgggtcgac gacgaaatcg atgacggaca
      781 ccgtcgcgcg gcgctcccat tccgtgccgt ccgcgctcac gctgaaggtc ccgtcggtgg
      841 agctccaccc gtacacgcgg tcgtgggccg cgcgcagcgc gtggaagtcc gcgacgccga
      901 gccgggacac gctctgccac gtcacaccgc cgtcgcgaga ctcgatcagg cccagcaggg
      961 gcggcaggtc ggtgcgcagg tccgggtgcc cgctggccag gaagtggtcg gggccgacca
     1021 cgctgaagcc catgaagtcg gcgtaggtgt cgccgacgcg ctccggcacg ccgtccgagg
     1081 ccagccgcca cagtcccgtg tgcgtcgcca cgtagacgcg ctcgtcggcg gggttgaggc
     1141 ccaggccgtg cacgtgcacg ggccccgggt cgtcagcggg cgggagcccc ggcgcggcac
     1201 cctcacggtt cgccagcccc cacgccagcc cgcccgcggc cagcagcgcg gcgatcagca
     1261 ccaaccggac ggccgggcgc aagagcggcc cgcgcgcatc gctcgtcatg gcggcgacga
     1321 cgtccatcac agggagcggg ccagggcgat cagctcctcc atcgacagtg ccgcgccctc
     1381 ccgccacagc gcgccttggc gctcgtcgag gtcggcggcc tcgaaccggc cgtcgtgggc
     1441 gttgaccagc cgcgccagat cggtgccggt gtcatggcgc agccgctcgg caccgccgcg
     1501 gatgcgcagc gcgtcgtcgc cctgcccggc gcgcgccgca cgagcggcga tgtcgtccag
     1561 cagcagcatc acgccggaga tgtcgcggtg ctcaacgaac acgtccagtc cctcgatgaa
     1621 cgcctcgcgg gcctccgcca ggcggtccag ccgcaccagc gacatcccca ccgcgtgcag
     1681 cgcccatccc gtgccgaacc agtcgccgtg ccggcggaac acctccaggc tctccgtgcc
     1741 gtaccccagc gcccgcacga agtcctcccg ggcctcgtcc gcctcgcccc gctccaggcg
     1801 ggccaccgcc ccgtcgtagg tgttggtcga catcgcccac aggcacttgg cggcgcccag
     1861 gtcggcgtgc gcctcccgat acagctccag ggcctgtccc agcagcgaca tcgtgcgccc
     1921 gatgtcgctg gcctggacgt cctcgacgcc gagctggtag gtgaacgcca ggttgtacag
     1981 cgcctcggcc acgcgctcgc cgtcgcccag ctcccgctcg aggtccagca cgcgctcgta
     2041 cgccgcgcgt gccgccggca tgtcggcctg ccagtacgcc agcccgccca gcgcctcgaa
     2101 gccgcgcgcc cgcagcgcgg catcggcgtc cgcgctcgcc gcgatgagcg cctccagccg
     2161 cgtgcgtccc tcgtgcaggt gcccgcgcat ctgccagacg cgccacagcg ccgccccgag
     2221 ccgcagcgcg cacgccgggt cccgcgcagc catccagtcc agcgcggcac ggaagttgtc
     2281 gagctcgccc tccagccggt ccagccacca cgcgccgcgc gggcccatca gctcgccggc
     2341 gccgtcctcg gccagcgcca ggaacgcctg gccgaggac
//
LOCUS       DATMKC010000453         6125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_251272, whole genome shotgun sequence.
ACCESSION   DATMKC010000453 DATMKC010000000
VERSION     DATMKC010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251272"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VNU01_13125"
     CDS             complement(<1..253)
                     /locus_tag="VNU01_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33605.1"
                     /translation="MPEETSHPRAAESEGALPPGYPAELVEQAWLVDGVQVLFRPVLP
                     DDRGRYERMFTRLSPETLYRRFMTPVTRVDQRLLSHLVQM"
     gene            276..1244
                     /gene="pfkA"
                     /locus_tag="VNU01_13130"
     CDS             276..1244
                     /gene="pfkA"
                     /locus_tag="VNU01_13130"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWH33606.1"
                     /translation="MRRIAVLTSGGDAPGMNAAVRAVVRAADAAGLETYRVRSGYEGL
                     IDGDLAPLDARAVSGVLHLGGTIIGTARSARFLTEQGRAQAAATLRDAAVDGLVVIGG
                     DGSFRGADALSAEHGVRVVGIPGTIDNDLSGTDWTLGFDTAVNTALESIDRIRDTAMS
                     HGRLFFVEVMGRRSGWLALYSALAGGATDVLIPERPTDIEAVRAKVRAAFAMGKGFCI
                     VVVAEGDDAGNVYEVAARVADGLGLDHRVTALGHVQRGGPPSMRDRLLGARLGDAAVT
                     ALREGADRVMVGEMGGEVLRTPLARTWTEARPAPAALLELLGRLAR"
     gene            complement(1241..1513)
                     /locus_tag="VNU01_13135"
     CDS             complement(1241..1513)
                     /locus_tag="VNU01_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWH33607.1"
                     /translation="MARRTIDGGVVLGMAGRDGREAMRAFVERHELGALTHLVDDDGS
                     LWERFAVPGQPAWVFLDRDGVIVDRHFGPLEPDTVLRRLRELARPS"
     gene            complement(1550..1786)
                     /locus_tag="VNU01_13140"
     CDS             complement(1550..1786)
                     /locus_tag="VNU01_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33608.1"
                     /translation="MRPRLLLALVALAAACAAPPGDGGGPTASVGTSPRAAASDGTAA
                     ADPLAGSAPTLAGGDLELSALRGQALALWFWAPW"
     gene            complement(1792..3102)
                     /locus_tag="VNU01_13145"
     CDS             complement(1792..3102)
                     /locus_tag="VNU01_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWH33609.1"
                     /translation="MSASLRSRRREIIVLAVPAMLALAADPLLSLIDTALVGRLGAAE
                     LAALGVASAVFTSLFWLFNFLTYGTTSEVARALGGGRPEEAARRVAQALWLALACGLL
                     VTALLQAATPAILAAMGAAGEVREHATAYLRVRALAAVPALVAMVGHGAYRGLKDTRT
                     PLRITVALNVADAALSWALIYPAGLGVAGAAWGTVVAQTAVAAAFLVLLRRRLPSAAA
                     RVDPAVMRAILRVSRDLFLRTASLLAGQLLSTAAAARMGTEALAAHQVARELWVLLAL
                     VLDGFAIAGQAMIATSLGAGDARTAKAEAVTLVRYGAATGLAIGVGYLLLIGALPGVF
                     SRDAGVLEQVRAVWPVVALLQPIGGVVFVLDGVLMGAHDYRYLLRTTALASLGVLAPV
                     SFLALGLGWGLTGVWAGMAGLMLVRLVALSLRLQRWDAVTATLR"
     gene            3101..3523
                     /gene="arfB"
                     /locus_tag="VNU01_13150"
     CDS             3101..3523
                     /gene="arfB"
                     /locus_tag="VNU01_13150"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWH33610.1"
                     /translation="MAGVRISRTLSIPQEEIELSFARSGGPGGQHVNTSATKVELRFD
                     VARSPSLSDEQRRLLLERLSSRLTNDGVLVLHAGGHRSQTRNREAAVERFAGLLREGL
                     KKRRPRTPTRPSRASKQRRLDEKKQHGEKKRLRRAPDA"
     gene            complement(3501..3887)
                     /locus_tag="VNU01_13155"
     CDS             complement(3501..3887)
                     /locus_tag="VNU01_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33611.1"
                     /translation="MPAELLSAWEAFQRQAERVEQARQALLRCLPVGRVEPAPIGLGL
                     DLLRDELHAVRAEMDAWRVPAAEQDWTACREAVDEALAHIEPARRVSLETGELEELLT
                     AVTDVVEPLDAWATAERHWLKRRVRA"
     gene            complement(4031..4213)
                     /locus_tag="VNU01_13160"
     CDS             complement(4031..4213)
                     /locus_tag="VNU01_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33612.1"
                     /translation="MAEQTSTLEEIARMRASTLVPKPEYQDVLVEGRVHEKSLTEVGD
                     EDEVDANKRIAVRLAM"
     gene            complement(4226..5059)
                     /locus_tag="VNU01_13165"
     CDS             complement(4226..5059)
                     /locus_tag="VNU01_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33613.1"
                     /translation="MTISVTIQVMDGMVFAADSTSTLMTSNGVAANTYDHADKILNLC
                     KGRPLAVGFWGMGGIGESSMMTLMKDLRARFAGDVDSYEEWLLKPTCSVREVAVRVKE
                     FIYDEKYVPVYGGNKKHPALGLAVGGYSPGKSLPERYEIIIGEDGQCQGPEEENPGSA
                     GSLRWRGQPEAITRLVLGFGLDLPSVLHEKLGVPEDQIGPAVDLIRANLLQDPVHPAM
                     PIQDAIDLAEFLVELTKKWVRFRPGAVTVGGPTEIAAITKHEGFKWVRRKHYYPQTLN
                     P"
     gene            complement(5150..5764)
                     /locus_tag="VNU01_13170"
     CDS             complement(5150..5764)
                     /locus_tag="VNU01_13170"
                     /inference="COORDINATES: protein motif:HMM:NF039596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWH33614.1"
                     /translation="MEPQRKRRPDPARAAHKAERRERLIDAVADALRRHGAGASMDEI
                     AADVGVAKPVLYRYFGSRHGLNLALADRVTGSLTWELQDALARDAGPRALLRTCIDTY
                     LRFVEREPELYRWLTQWARTEQPHAVTGLMAQLGDEIGKVLAERLAAFGLDPAPAATW
                     AYGIVGMVHLAGERWLDRQDVPRERLADDLVTLLWSGFAGYAAG"
     gene            5828..>6125
                     /locus_tag="VNU01_13175"
     CDS             5828..>6125
                     /locus_tag="VNU01_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWH33615.1"
                     /translation="MDFALSEEHLELQKWAHEFAVREIRPVASEWDEREEFPWPVLQK
                     AAEAGIYGLDIYLQHQADPTQLTLPVVMEEVFWGCAGIGLAIFGTGLALAALAGS"
BASE COUNT          908 a         2259 c         2160 g          798 t
ORIGIN      
        1 ccatctgcac caggtgcgac aacaggcgct ggtcgacacg cgtgaccggc gtcatgaagc
       61 gccggtacag cgtctccggc gacaggcgcg tgaacatgcg ctcgtagcgg ccgcggtcgt
      121 ccggcagcac gggacggaac agcacctgga cgccgtcgac cagccacgcc tgctcgacca
      181 gttccgccgg gtagccaggc ggcagcgcgc cctcggactc tgcggcgcgg gggtgggagg
      241 tttcctcggg catcgtcttg gtccaggctg gggccgtgcg acgcatcgcg gtgctgacca
      301 gcggcggtga cgcgcccggc atgaacgccg cggtgcgcgc cgtggtgcgc gcggccgacg
      361 ccgctgggct ggaaacctac cgcgtgcgca gcggctacga gggcctcatc gacggcgacc
      421 tggcgccgct ggacgcgcgc gcggtctccg gcgtgctgca cctgggcggc acgatcatcg
      481 gcaccgcccg ctcggcgcgc ttcctcacgg agcagggcag ggcgcaggcg gcggccacgc
      541 tgcgcgacgc cgccgtcgac ggcctggtgg tcatcggcgg cgacggctcc ttccgcggcg
      601 ccgacgcgct gtccgccgag cacggcgtgc gcgtggtggg catcccgggc accatcgaca
      661 acgacctgtc cggcacggac tggacgctgg gcttcgacac cgcggtcaac acggcgctgg
      721 agtccatcga ccgcatccgc gacaccgcga tgtcgcacgg acggctgttc ttcgtggagg
      781 tcatggggcg ccgctccggc tggctggcgc tgtactcggc gctggccggc ggcgccaccg
      841 acgtgctcat ccccgagcgg cccaccgaca tcgaggcggt gcgggccaag gtgcgcgccg
      901 cgttcgccat gggcaagggc ttctgcatcg tggtggtggc ggaaggcgac gacgccggca
      961 acgtctacga ggtcgccgcc cgcgtggccg acgggctggg cctggaccac cgcgtcaccg
     1021 cgctcggcca cgtgcagcgc ggcgggccgc cgagcatgcg cgaccggctc ctgggcgcgc
     1081 ggctggggga cgcggccgtg acggcgctgc gcgagggcgc cgaccgcgtg atggtgggcg
     1141 agatgggcgg cgaggtgctg cgcacgccgc tggcgcgcac gtggaccgag gcgcggcccg
     1201 cgcccgccgc cctgctggag ctgctgggcc gcctggcgcg ctaggagggc cgcgcgagct
     1261 cgcgcaacct gcgcagcacc gtgtccggct cgagcgggcc gaagtggcgg tcgacgatca
     1321 cgccgtcgcg atccaggaac acccacgcgg gctgccccgg caccgcgaag cgctcccaca
     1381 gcgagccgtc gtcgtccacg aggtgcgtga gcgcgccgag ctcgtgccgc tccacgaagg
     1441 cacgcatggc ctcgcgtccg tcgcgccccg ccatgccgag caccacgccg ccgtcgatcg
     1501 tccgccgcgc caactgcgcg accccggggg cttcccggtt gcaggtcggt caccacggcg
     1561 cccagaacca cagcgccagt gcctgcccgc gcagggctga cagctccagg tcgccgcccg
     1621 cgagcgtcgg cgcgctgccc gccagcgggt cggccgcggc ggtgccgtcg gaggcggcgg
     1681 cccgcggcga cgtgccgacg gaggcggtgg ggccgccacc gtcaccgggg ggcgccgcgc
     1741 acgccgccgc caacgcgacg agggccagca gcagacgggg gcgcatggcc atcaccgtag
     1801 cgtggcggtg accgcgtccc agcgctgcag ccgcagcgac agggccacca ggcgcacgag
     1861 catcagcccg gccatgccgg cccacacgcc ggtcaggccc cagcccaggc ccagggccag
     1921 gaagctcacc ggcgcgagca cgcccagcga cgccagcgcc gtggtgcgca gcaggtagcg
     1981 gtagtcgtgc gcgcccatca gcaccccgtc cagcacgaac accacgccgc cgatcggctg
     2041 cagcagggcc acgaccggcc acaccgcgcg cacctgctcc agcacgcccg cgtcgcggct
     2101 gaacaccccc ggcagcgctc cgatgagcag caggtatccc acgccgattg ccagccccgt
     2161 ggccgcgccg tagcgcacca gcgtgacggc ctccgccttg gcggtgcgcg cgtcccccgc
     2221 gcccagcgac gtcgcgatca tcgcctggcc cgcgatcgcg aatccgtcca gcaccagcgc
     2281 cagcagcacc cacagctcgc gggcgacctg gtgcgcggcc agcgcctcgg tgcccatgcg
     2341 cgccgccgcg gcggtggaca ggagctggcc ggccagcagg ctcgccgtgc gcaggaacag
     2401 gtcgcgcgac acccgcagga tggcgcgcat gaccgccggg tccacgcgcg ccgcggcgga
     2461 cggcaaccgt cgccgcaaca gcaccaggaa ggcggccgcc acggcggtct gcgcgacgac
     2521 ggtgccccag gcggcgccgg cgacgccgag gccggccggg tagatcagtg cccaggacag
     2581 cgccgcgtcg gccacgttga gcgccaccgt gatgcgcagc ggcgtgcgcg tgtccttgag
     2641 tccccggtag gcgccgtggc cgaccatcgc gaccagggcg ggcactgcgg ccagcgcgcg
     2701 cacacgcagg tacgcggtgg cgtgctcgcg cacctcgccg gccgcgccca tcgccgccag
     2761 gatcgcgggt gtggccgctt gcagcagcgc cgtgaccagc aggccgcacg ccagcgcgag
     2821 ccacagcgcc tgcgccacgc ggcgggcggc ctcctccggg cggccgccgc cgagcgcgcg
     2881 cgcgacctcg gaggtggtgc cgtaggtgag gaagttgaac agccagaaca gcgaggtgaa
     2941 cacggcgctg gccacgccga gcgcggccag ctccgccgcg ccgagccggc cgaccagcgc
     3001 ggtgtcgatc agcgacagca gcgggtcggc cgccagcgcc agcatcgccg gcaccgccag
     3061 gacgatgatc tcgcgacggc ggctgcgtag gcttgcgctc atggccggtg tccgcatctc
     3121 gcgcacgcta tcgatcccgc aggaggagat cgagttgtcc ttcgcgcgct ccggcgggcc
     3181 agggggccag cacgtcaaca cctccgccac caaggtggag ctgcgcttcg acgtggcgcg
     3241 cagcccgtcg ctcagcgacg agcagcgccg gctgctgctg gagcggctgt cgtcgcgcct
     3301 gaccaacgac ggcgtgctgg tgctgcacgc cggcggtcac cgctcgcaga cacgcaaccg
     3361 cgaggcggcg gtcgagcgct tcgccggcct gctgcgcgag ggcctgaaga agcggcgtcc
     3421 ccgcacgccg acacgaccct cgcgggcctc caagcagcgc cgcctggacg agaagaaaca
     3481 gcacggcgag aagaagcgcc tcaggcgcgc acccgacgct tgagccagtg ccgctcggcg
     3541 gtggcccagg cgtccagcgg ctccaccacg tcggtgacgg cggtgagcag ctcctccagc
     3601 tcaccggtct ccaggctcac ccggcgggcc ggctcgatgt gggcgagcgc ctcgtcgacg
     3661 gcctcccggc aggccgtcca gtcctgctcg gcggcgggca cgcgccaggc gtccatctcg
     3721 gccctgacgg cgtggagctc gtcgcgcagc aggtccaggc ccaggccgat gggcgcgggc
     3781 tccacccggc cgacgggcag gcagcgcagc agcgcctggc gggcctgctc gacgcgctcg
     3841 gcctggcgct ggaacgcctc ccaggcggac aggagctcgg cgggcatggt tttcttccgc
     3901 agcatcacgg cgtgagagta ggcgcgcgca cgtgccgact gaaatcccaa cgacgcgtga
     3961 aggcccgtcc aaccgggcgt ctaccgcttg gcggagcccc ggaagcagca gcggtcaggg
     4021 ccgccccgtg tcacatcgcc agacgaacgg cgatccgctt gttggcgtcg acctcgtctt
     4081 catctccgac ctcggtcaaa gacttttcgt gcacgcggcc ctcaaccagt acgtcctggt
     4141 actcaggctt gggaacgagt gtggaagcgc gcatccgcgc gatctcttcc aaagtagacg
     4201 tctgttcagc cattgcgcac caccctcatg ggttcaacgt ctgcggatag tagtgcttcc
     4261 gacgcaccca cttgaaaccc tcgtgcttcg ttatggctgc gatctcggtc ggtccaccga
     4321 ccgtcacagc gccaggtcgg aaccggaccc acttcttcgt cagttccacc aaaaactcgg
     4381 caagatcgat cgcgtcctgg ataggcatcg ccgggtgaac cggatcttgt aataggttcg
     4441 cgcggatcag gtccacagca ggtccaatct gatcctcggg caccccaagc ttctcgtgaa
     4501 gcacggacgg cagatccagt ccgaagccaa gcaccaaacg ggtgattgcc tccggctgac
     4561 cacgccaacg aagggatcca gcgctgcctg ggttctcctc ttccggtccc tggcactgac
     4621 cgtcttcgcc aatgattatc tcgtagcgct cgggtagtga cttgcccggc gagtagccgc
     4681 cgacggccaa gccgagcgcc gggtgcttct tgttgccacc gtagacgggg acgtacttct
     4741 cgtcgtagat gaactccttc acccggacgg cgacctcacg aacgctgcat gtgggcttca
     4801 ggagccactc ttcgtaggag tcgacatcgc ctgcgaagcg tgcgcgcaga tccttcatca
     4861 gagtcatcat cgaggattcc ccgataccgc ccatgcccca gaagcccacc gccaacggac
     4921 gccccttgca cagattgagg atcttgtcgg cgtggtcata ggtgttcgcc gccacgccat
     4981 ttgacgtcat gagtgtggac gtgctgtcgg ccgcgaagac catcccgtcc atcacctgaa
     5041 tcgtgacgga aatcgtcatg ccacgcccgc tccccgttct gacttcgggc tccatccgac
     5101 ccgtatagct cggatgctaa ccggaggcag tgacgcaccc ggtaggcctt cagccggcgg
     5161 cgtagcccgc gaaccccgac cacagcagcg tgacgagatc gtcggccagg cgctcgcggg
     5221 gcacgtcctg gcggtccagc cagcgctccc ccgccaggtg caccatgccg acgatgccgt
     5281 acgcccacgt cgccgccggc gcggggtcga ggccgaaggc ggccagccgc tccgccagga
     5341 ccttgccgat ctcgtcgccg agctgggcca tgagcccggt gacggcgtgc ggctgctccg
     5401 tccgcgccca ctgggtgagc cagcggtaca gctccggctc gcgctccacg aagcgcaggt
     5461 aggtgtcgat gcaggtgcgc agcagcgcgc gcggcccggc gtcgcgcgcc agggcatcct
     5521 gcaactccca ggtgagtgag ccggtgaccc ggtcggccag cgccaggttc aggccgtggc
     5581 gggagccgaa gtagcggtac agcaccggct tggccacgcc cacgtcagcg gcgatctcgt
     5641 ccatggacgc tccggcgccg tggcggcgca gcgcgtcggc gaccgcgtcg atgagccgct
     5701 cacgacgctc ggccttgtgc gccgccctcg ccgggtccgg tcgccgcttg cgctgtggct
     5761 ccatgctacg catagtatct gttacgagtc gtaacaccta aggggcaccg ccgcacagga
     5821 ggagcacatg gacttcgcgc tcagcgagga gcacctggaa ctgcagaagt gggcgcacga
     5881 gttcgccgtg cgcgagatcc ggcccgtcgc ctccgagtgg gacgagcgcg aggagttccc
     5941 ctggccggtg ctgcagaagg ccgccgaggc cggcatctac ggcctggaca tctacctgca
     6001 gcaccaggcc gacccgacgc agctcacgct gccggtggtc atggaggagg tcttctgggg
     6061 ctgcgcgggc atcggcctgg cgatcttcgg cacgggcctg gcgctggcgg cgctggccgg
     6121 cagcg
//
LOCUS       DATMKC010000454         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_309644, whole genome shotgun sequence.
ACCESSION   DATMKC010000454 DATMKC010000000
VERSION     DATMKC010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309644"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VNU01_13180"
     CDS             complement(<1..962)
                     /locus_tag="VNU01_13180"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWH33616.1"
                     /translation="MISREPTLERLATAQRLLLEPFGLDETHLARALAEITAHRVDDA
                     DLYFQYTRSEGWSLEEGIVKTGSFSIDTGVGVRAISGEKTAFAYSDDISEASLLDAAR
                     TVRTIAAAGGEGRARVGEKKIAGGRSLYGPVDPIASLDSTAKVQLLERAEKKARAKDP
                     RVVQVMAGLASEYDVVMVARADGTLAADIRPLVRLSVTVIAEQNGRREMGTGGGGGRF
                     GLAYFDEARIDEFVDDAVSAALTNLESRPAPAGEMTVVLGPGWPGILLHEAIGHGLEG
                     DFNRKGSSAFAGRVGKRVAAKGVTVLDDGTIADRRGSLNVDDEG"
     gene            complement(1008..>2474)
                     /locus_tag="VNU01_13185"
     CDS             complement(1008..>2474)
                     /locus_tag="VNU01_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWH33617.1"
                     /translation="AQVAPAVLEDGRHLRFIGDGLLAVFSNTMAGGSPLPAARRAVSA
                     ALGMALATHEFRSWLVQRFAQRGLPPFAIGIGLHCGEVTICRLGTTQNKETTPIGDTV
                     NMAARLEAASKELGWTVVASTAVLHEAGEGVQTGGITSLEVRAQNVFMEVAEIVGLET
                     NLEDRTHGMATLVGRAEEVRNAVQINSEITARAVKGALRSKLSAFKGHSFDSGEPLRL
                     KGYRLTRKIGSGGMTEVFLAERESDRLPVVLKVLDASGKATGEHLARFIQEYTLLSRI
                     EHPHVIRIYDQGFTDDHAYIAMEYFERGDLRAEIKAGMAPPRVLEIVRQVAQALEAVH
                     ARGIIHRDLKPENIMRRPDGSVALADFGIAKSMLQENLALTQTRHGDVVGTPYYLSPE
                     QASGQAITPQSDLYSLGVMMFEMLTGARPFRAESLDLLLARHLSAPTPDLPAAVSELQ
                     PVVNRLMAKKPAERYASAQALLDDLDAHYLNRTIELRR"
BASE COUNT          323 a          904 c          850 g          397 t
ORIGIN      
        1 ttgccctcgt cgtcgacgtt gagcgagccg cggcggtccg cgatcgtgcc gtcgtcgagc
       61 acggtcacgc ccttggcggc cacgcgcttg cccacccgcc cggcaaacgc gctggagccc
      121 ttgcggttga aatcgccctc cagcccgtgg ccgatggctt cgtgcaacag gatgccgggc
      181 cagccggggc ccagcacaac ggtcatctcg cccgcgggcg ccgggcgcga ctccaggttc
      241 gtcagcgccg cgctgaccgc gtcgtccacg aactcgtcga tgcgcgcctc gtcgaaatac
      301 gccaggccga agcggccgcc gccgccgccg gtgcccatct cgcgccggcc gttctgctcg
      361 gcgatgaccg tgaccgacag ccgcaccagc ggccggatgt ccgccgcgag cgtgccgtcc
      421 gcccgggcga ccatcaccac gtcgtattcg ctcgccaggc cggccatcac ctggaccacc
      481 ctggggtcct tcgcgcgcgc cttcttctcg gcgcgctcga gcagctgcac cttggccgtg
      541 ctgtccaggc tggcgatcgg atcgacgggg ccgtagagcg accgcccgcc ggcgatcttc
      601 ttctcgccga cgcgggcgcg cccttccccg ccggccgcgg cgatggtgcg caccgtgcgc
      661 gccgcgtcca gcagcgaggc ttcggagatg tcgtccgagt aggcgaatgc cgtcttctcg
      721 ccgctgatcg cccgcacgcc cacgccggtg tcgatgctga agctgccggt cttgacgatg
      781 ccctcttcca ggctccagcc ttcactgcgg gtgtactgga agtagaggtc ggcgtcgtcg
      841 acccggtgcg cggtgatctc cgcgagcgcg cgcgcgaggt gggtttcgtc caggccgaag
      901 ggttccagca gcagccgctg ggcggtggcc agccgctcga gggtgggttc acgcgagatc
      961 atcggcccat tctaggacgg tgcccgtttc cgtgccgctc gccggtgcta tcgccgcagc
     1021 tcgatggtcc ggttgaggta gtgcgcgtcc aggtcgtcca gcagcgcctg cgccgacgcg
     1081 tagcgctcgg ccggcttctt cgccatcagc cggttcacca ccggctgcag ctcggacacc
     1141 gctgccggca ggtccggggt gggcgcggag aggtggcgcg ccagcagcag gtccagcgac
     1201 tcggcccgga aaggccgcgc ccccgtgagc atctcgaaca tcatcacgcc caggctgtac
     1261 aggtccgact gcggcgtgat cgcctggccc gaagcctgct ccgggctcag gtagtagggc
     1321 gtgccgacca cgtcgccgtg gcgcgtctgc gtgagcgcga ggttctcctg cagcatggac
     1381 ttggcgatgc cgaagtcagc cagcgcgacc gagccatccg ggcgccgcat gatgttttcc
     1441 ggcttgaggt cgcggtggat gatgccgcgg gcgtggacgg cttccagcgc ctgcgccacc
     1501 tgccgcacga tctccagcac gcgcggcggc gccatgcccg ccttgatctc cgcgcgcagg
     1561 tcgccgcgct cgaagtactc catcgcgatg taggcgtggt cgtcggtgaa gccctggtcg
     1621 tagatgcgga tcacgtgcgg atgctcgatg cgcgagagca gcgtgtactc ctggatgaag
     1681 cgcgccaggt gctcgccggt ggccttgccg ctcgcatcca gcaccttcag cacgacgggc
     1741 agccggtcgg actcgcgctc ggccaggaag acctccgtca tgcccccgga gccgatcttg
     1801 cgcgtgaggc ggtagccctt caggcgcagc ggctcgccgc tgtcgaagga gtggcccttg
     1861 aacgccgaca gcttggagcg caaggcgccc ttgacggccc gcgccgtgat ctccgagttg
     1921 atctgcaccg cgttgcgtac ctcctcggct cgcccgacca gcgtggccat gccgtgcgtc
     1981 ctgtcctcga ggttggtctc cagccccacg atctcggcga cctccatgaa gacgttctgc
     2041 gcccgcacct cgagcgaggt gatgccgccg gtctgcacgc cttcgcccgc ctcgtggagc
     2101 accgccgtgc tggcgaccac ggtccagccc agctccttgc tggccgcttc cagccgcgcg
     2161 gccatgttga cggtgtcgcc gatcggcgtc gtctccttgt tctgcgtggt cccgaggcgg
     2221 cagatcgtca cttccccgca atgcagcccg atgccgatgg cgaagggcgg caggccgcgc
     2281 tgcgcgaagc gctggaccag ccaggaccgg aattcgtgcg tggccagcgc catgcccagc
     2341 gccgcggaga ccgcgcggcg cgccgcgggc agcggcgagc cgccggccat ggtgttcgag
     2401 aacacggcca gcaggccgtc gccgatgaag cgcaggtggc gcccgtcctc aagaacggcg
     2461 ggcgccacct gcgc
//
LOCUS       DATMKC010000455         5643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_61665, whole genome shotgun sequence.
ACCESSION   DATMKC010000455 DATMKC010000000
VERSION     DATMKC010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61665"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VNU01_13190"
     CDS             complement(<1..1312)
                     /locus_tag="VNU01_13190"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWH33618.1"
                     /translation="MSLFDELVAGCPDLDDAAIDHLQALLGEWQILADLSFADLLLWV
                     RDELPTGSMGYRCVGQMRPYTAQTIYHEDMVGAVVPATSRPMVGRAYAEARVIRETDL
                     DWSTGVPLREEAIPVHFNGEVVAVVTREANISTARSPSQLELTYLQSAGELAQMLAEG
                     TFPYAIDQLDEREVSPRVGDGLLRLDAEGRVVYASPNAVSAYRRLGSIDLSTGQHVGD
                     FDPVAEELLEGLAHAEPVETEVERHGTIVVRRLLPLIGEGRSRAGLLLVREVTDIRRQ
                     ERALRVKDATIREIHHRVKNNLQTVASLLRLQARRLRSDEARAALAESVRRISSIALV
                     HETLSQETRQRVDFVKVSERIIAMLADGLVSPEAPITFDQEGEPGELSAEVATPLALV
                     LTELLQNCVEHGFPVRQPAARRGGSVRVSYERGARALRVVVRDDG"
     gene            complement(1336..3936)
                     /locus_tag="VNU01_13195"
     CDS             complement(1336..3936)
                     /locus_tag="VNU01_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH33619.1"
                     /translation="MLVVGMLAVGLPPAAAAELPPAAPTVVAAMAAPPALDARTSAPV
                     AAPVPFSLVGFRVPAGAELQVRASANGEDWSGWIEAEPLPADEGPDPGGPEPAVPSEG
                     LVPTAPVWTGAARHLQVRVEGATPQQVLPTVIDTLGQSRSLPGRMWDAVRAAWRPAAA
                     HAAAGAPSIVSRAGWGADESWRDGSPSYSRRVRAAFVHHTVNANSYSQAEAPALVRGI
                     YRYHTQSLGWSDIGYQFLVDRFGTVYEGRAGGVDRPVLGAHAGGFNTSTFGVASLGTH
                     TSAGPGSGALESIARVIAWKFDIHHVDVTGSVELTSRGSTRYPSGQRVRLATVSGHRA
                     VSQTSCPGQALDSLLPAIRHRVLELGGRMILDHDASPLRLRVARGQSVDGPVRFSARL
                     RPAGEWALEVRAPSGAVVHRDSGSGEAVASTWTPPPAVALGRYTWTVSSEGRTPAQEY
                     VDLVLPVIEDARSGSGVARGGKDGGFASPMTFSARLWPNAAWRLSVTDPSGTPAHHAA
                     GVGEQAAATWAGRAGLTPGRYTWRLEADEAAPASGTFEVLWPVLARSAVAADAPGEAV
                     AVSGLAFGPGQAAHAVVARADLFADAMAGGPLAGADGPVLLTPGDRLDDRVWAELERV
                     LPAGRTVYVLGGERAVSPAVAQALAGRWRVVRLAGSGRAETAVRIAELVLERRGGDRV
                     LIARAGPDSAVPWADALAGGAYGAWAGVPVLLTDRDALAPAAAELIVRRGIRRSVVLG
                     GPSAVADAVLDRLPQPTRVFGQDRAGTAAAVAEQLWGRTGGRGGDRLIVANGFAADAW
                     TLPLAAAPLAARNGAPLLLADRDTVPPATLQHLDRLRYTPERAASGWLLGGEGRVGQA
                     AADEVMRRLQ"
     gene            complement(4112..4405)
                     /locus_tag="VNU01_13200"
     CDS             complement(4112..4405)
                     /locus_tag="VNU01_13200"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWH33620.1"
                     /translation="MSDERPPVRRPRSDEAEDAALRALRQKLSGVLSRLPQIERQVLE
                     LRMGLKEGVPAKPGEVATRLGLTIAEVKKIETRAFQRIREVGPIKGLERFLER"
     gene            4496..5515
                     /locus_tag="VNU01_13205"
     CDS             4496..5515
                     /locus_tag="VNU01_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33621.1"
                     /translation="MDHAELQRLVDQADDAALLRAVDGLAASREWDLLETLRERCEQA
                     VEMGRQLWGVAMHVDYRLAWEGPAAHAARAVERGSSRFTLGPLTEVASSTHEWAALEV
                     HLRDPVVRATVAQERALRGEDLRGDASLAAYASLPAAVYRWEPDYALPVYRDRAAKFA
                     QPDAAFRHVPLTAAGGSASEPFDDEATDALRDVVRTWWTQSAGRGAALGVEGDAAGAV
                     RALRGERPGGFGLMAVESAEGLALLQWAGASGGAYGPRPGGAAGRFAAWWAAAALTGL
                     DWPERVDDVFAGELGAACAQLRWYRWAGTEPDTGWTLRLAIEDPTDGLAWCLEAVDQR
                     EDEEA"
BASE COUNT          678 a         2132 c         2188 g          645 t
ORIGIN      
        1 ccccgtcgtc gcgcacgacc acccgcaggg cgcgcgcccc gcgctcgtag gacacccgca
       61 cgctgccgcc ccggcgggcc gccggctggc gcaccgggaa gccgtgctcg acgcagttct
      121 gcagcagctc ggtcagcacg agcgccagcg gcgtggcgac ctccgcggac agctcgcccg
      181 gctcgccctc ctggtcgaac gtgatgggcg cctccgggct caccagcccg tcggcgagca
      241 tcgcgatgat gcgctccgac accttcacga agtcgacgcg ctggcgggtc tcctggctca
      301 gcgtctcgtg caccagggcg atggaggaga tgcggcgcac gctctcggcc agcgccgcgc
      361 gggcctcgtc gctgcgcagg cgccgcgcct gcaggcgcag caggctcgcc accgtctgca
      421 ggttgttctt cacgcgatgg tggatctcgc ggatggtggc gtccttgacg cgcagcgccc
      481 gctcctggcg gcggatgtcg gtgacctccc gcaccagcag caggcccgcc cgtgagcgcc
      541 cctcgccgat gagcggcagg aggcggcgca ccacgatggt gccgtgccgc tccacctccg
      601 tctcgaccgg ctcggcgtgg gccagcccct ccagcagctc ctcggccacg gggtcgaagt
      661 cgccgacgtg ctggcccgtg gacaggtcga tggagcccag ccgccggtac gccgacacgg
      721 cgttgggcga ggcgtacacg acgcgcccct cggcgtccag ccgcagcagg ccgtcgccga
      781 cgcgcgggga cacctcgcgc tcgtcgagct ggtcgatggc gtaggggaac gtgccctccg
      841 ccagcatctg cgccagctcg ccggcgctct gcaggtaggt cagctcgagc tgcgacggcg
      901 agcgggccgt ggagatgttc gcctcgcggg tgaccaccgc gaccacctcg ccgttgaagt
      961 ggaccgggat cgcctcctcg cgcagcggca cgccggtgga ccagtccagg tcggtctcgc
     1021 ggatgacgcg cgcctccgcg taggcgcgcc ccaccatcgg ccgcgacgtc gcggggacca
     1081 ccgcgcccac catgtcctcg tggtagatcg tctgcgcggt gtaggggcgc atctggccga
     1141 cgcagcggta gcccatgctg ccggtcggca gctcgtcgcg gacccacagc agcaggtcgg
     1201 cgaaggacag gtccgccagg atctgccatt cgccgagcag cgcctggagg tggtcgatgg
     1261 cggcgtcgtc gaggtcggga cacccggcca cgagctcgtc gaagagcgac atgggcgcga
     1321 tcctatccgg gtgcgctact gcagccggcg catgacctcg tccgcggccg cctggccgac
     1381 gcggccctcc ccgcccagga gccagccgga ggccgcgcgc tccggcgtgt agcgcagccg
     1441 gtccaggtgc tgcagcgtcg cgggcggcac ggtgtcgcgg tcggccaaca gcagcggcgc
     1501 gccgttgcgc gccgcgagcg gcgcggccgc cagcggcagc gtccaggcgt cggcggcgaa
     1561 gccgtttgcc acgatcagcc ggtcgccgcc gcggccgccg gtgcggcccc acagctgctc
     1621 ggccacggcc gcggcggtcc ccgcgcggtc ctggccgaac acgcgggtgg gctgcggcag
     1681 ccggtccagg accgcgtcgg cgacggcgga cgggccgccc aggacgacgc tgcggcggat
     1741 gccgcggcgc acgatcagct cggccgccgc gggcgccagc gcgtcacggt cggtgagcag
     1801 gacgggcacg ccggcccagg cgccgtacgc gccgcccgcg agcgcgtcgg cccagggcac
     1861 cgccgagtcc ggcccggcgc gggcgatcag gacgcggtcg ccaccacggc gctccagcac
     1921 cagctcggcg atgcgcacgg cggtctcggc gcggcccgag ccggccagcc gcaccacgcg
     1981 ccagcggccg gcgagggcct gcgcgacggc ggggctgacc gcccgctcgc cgcccaggac
     2041 gtacacggtg cgtcccgccg gcaggacgcg ctccagctcg gcccagacgc ggtcgtccag
     2101 gcggtctccc ggcgtgagca ggaccgggcc gtccgcgccc gccagcggac cgccggccat
     2161 cgcgtcggcg aacaggtccg cgcgcgcgac caccgcgtgc gccgcctggc cgggcccgaa
     2221 cgccaggccg gacaccgcca ccgcctcgcc cggcgcgtcg gccgcgacgg cgctgcgcgc
     2281 cagcaccggc cacagcacct cgaacgtgcc ggaggcgggg gcggcctcgt cggcctcgag
     2341 ccgccaggtg tagcggccgg gggtcagccc ggcacggccc gcccacgtcg ccgccgcctg
     2401 ctcgcccacg cccgcggcgt ggtgcgccgg cgtgccggag gggtccgtga cgctcagccg
     2461 ccaggccgcg ttcggccaca ggcgcgcgct gaacgtcatc ggcgacgcga agccgccgtc
     2521 cttgccgccg cgcgccacgc cgctgccgct gcgcgcgtcc tcgatcacgg gcagcaccag
     2581 gtcgacgtac tcctgcgccg gcgtgcgccc ctcgctgctg accgtccagg tgtagcgccc
     2641 cagcgcgacg gcgggaggcg gcgtccaggt gctcgccacg gcctcgccgg agccgctgtc
     2701 gcggtgcacg accgcgccgg agggggcgcg cacctccagt gcccactccc ccgccgggcg
     2761 caggcgggcg ctgaagcgca ccggcccgtc cacggactgg ccccgcgcga cgcgcagccg
     2821 cagcgggctg gcgtcgtggt ccaggatcat gcgcccgccc agctccagga cgcggtggcg
     2881 gatggccggc agcaggctgt ccagcgcctg gccggggcag ctggtctgcg acacggcgcg
     2941 atggccgctc accgtggcca ggcgcacgcg ctggccggac gggtagcgcg tggagccgcg
     3001 gctggtcagc tccacggagc cggtgacgtc cacgtggtgg atgtcgaact tccacgcgat
     3061 gacgcgggcg atcgactcca gcgcccccga gccggggccg gcgctggtgt gggtgccgag
     3121 cgaggcgacg ccgaaggtgg aggtgttgaa gccgccggcg tgcgccccca ggacggggcg
     3181 gtccacgccg ccggcgcggc cctcgtagac ggtgccgaag cggtccacca ggaactggta
     3241 gccgatgtcg gaccagccca gcgactgcgt gtgatagcgg tagatccccc gcaccagggc
     3301 gggggcctcg gcctggctgt agctgttggc gttgacggtg tggtgcacga aggcggcgcg
     3361 cacccgccgc gagtagctgg gcgagccgtc gcgccaggac tcgtcggccc cccagccggc
     3421 gcggctcacg atgctcggcg caccggcggc ggcgtgcgcc gcggcgggac gccaggcggc
     3481 ccgcaccgcg tcccacatgc gcccgggcag cgagcggctc tggcccagcg tgtcgatgac
     3541 ggtgggcagc acctgctgcg gcgtggcgcc ctcgacgcgg acctgcaggt gacgggcggc
     3601 gccggtccac acgggcgccg tggggaccag gccctccgac ggcacggccg gctcggggcc
     3661 gccggggtcg gggccctcgt cggcgggcag gggctccgcc tcgatccagc cgctccagtc
     3721 ctcgccgttc gcggaggcgc ggacctgcag ctcggcgccg gcggggacgc ggaagcccac
     3781 cagggagaac gggacgggtg cggcgaccgg ggcggaggtg cgcgcgtcca gcgccggcgg
     3841 ggcggccatg gcggccacga ccgtcggggc ggccgggggc agctcggcgg ccgcggcggg
     3901 cggcaggccg accgcgagca tgccgacgac gagcacggtc agaagtgctc ggggaagcaa
     3961 cgtgactcct caccagccgg tccagcgaat gacggtgccc cgagcctcgg gcacagctcc
     4021 ggatcttttt gcgcccgagg cccagggcac tcagtcgcta ggacgcgcgg agcccggcag
     4081 gtgttccgca aaaatcgccc gcgggcgcgc gtcagcgctc caggaagcgc tcgagcccct
     4141 tgatgggccc gacctcgcgg atgcgctgga aggcgcgcgt ctcgatcttt ttgacctcgg
     4201 cgatggtcag gccgagccgt gtcgcgacct cgcccggctt ggcgggcacg ccctccttga
     4261 ggcccatgcg cagctccagg acctgccgct cgatctgcgg cagccggctg agcaccccgc
     4321 tgagcttctg gcgcagcgcg cgcagcgcgg cgtcctcggc ctcgtcggac cggggacgtc
     4381 tcacgggggg gcgctcgtca ctcatgaacc gctcatccta cacagcaccg gtcgcaggca
     4441 tccgcgcgcg ggcggcgggc cccgccgggc agcggcgcct ctaggcttgg ccgccatgga
     4501 ccacgccgaa ctgcagcgcc tggtcgacca ggccgacgac gccgcgctgc tgcgcgccgt
     4561 ggacgggctg gccgcgtcgc gggagtggga cctgctggag acgctgcgcg agcgctgcga
     4621 gcaggccgtc gagatgggcc ggcagctgtg gggcgtggcc atgcacgtcg actaccgcct
     4681 ggcgtgggag ggaccggccg cgcacgccgc ccgcgccgtg gagcggggat ccagccgctt
     4741 caccctgggg ccgctcacgg aggtcgcgtc gagcacgcac gagtgggcgg cgctggaggt
     4801 gcacctgcgc gatccggtgg tgcgggccac ggtggcgcag gaacgcgcgc tgcgcggcga
     4861 ggacctgcgc ggcgacgcgt cgctggcggc gtacgcgtcg ctgcccgcgg cggtgtaccg
     4921 gtgggagccg gactacgcgc tgcccgtcta ccgcgaccgc gccgccaagt tcgcccagcc
     4981 ggacgccgcg ttccgccacg tcccgctgac cgcggcgggc ggcagcgcgt ccgagccgtt
     5041 cgacgacgag gccaccgacg cgctgcgcga cgtggtgcgg acctggtgga cgcagtcggc
     5101 cggccgcggc gcggcgctgg gcgtggaggg cgatgccgcc ggcgcggtgc gcgcgctgcg
     5161 gggcgagcgt ccgggcggct tcggcctgat ggcggtcgag tcggccgagg gcctggcgct
     5221 gctgcagtgg gcgggcgcca gcggcggcgc gtacgggcca cggcccggtg gcgccgcggg
     5281 acgcttcgcg gcgtggtggg cggcggcggc gctcaccggc ctggactggc cggagcgcgt
     5341 ggacgacgtg ttcgccgggg agctcggcgc cgcgtgcgcg cagctgcgct ggtaccgctg
     5401 ggcgggcacg gaaccggaca ccggctggac cctgcgcctg gccatcgagg accccaccga
     5461 cggcctcgcc tggtgcctcg aagccgtcga ccagcgcgaa gacgaggagg cctagcggcc
     5521 cttccactcc ggagcgcgct tctgcaggaa ggccatcacg ccctccttca cgtcgtcggt
     5581 ctgcaggtgc agcgacagca tcgacgcgag gtagctcagc gcctggtccc ggcccatctg
     5641 cag
//
LOCUS       DATMKC010000456         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_251760, whole genome shotgun sequence.
ACCESSION   DATMKC010000456 DATMKC010000000
VERSION     DATMKC010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251760"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VNU01_13210"
     CDS             complement(<1..379)
                     /locus_tag="VNU01_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33622.1"
                     /translation="MREVFLVEAVRTPVGRIGGALRGVRPDDLLALTLRTLVDRAGLD
                     PAEVDEVIAGDTNQAGEDNRNVARMSLLLAGFPVTVPGATVNRLCASGLEAVVQGARH
                     GDGEAGQQQAHARDVAVVLARLVG"
     gene            504..1280
                     /locus_tag="VNU01_13215"
     CDS             504..1280
                     /locus_tag="VNU01_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWH33623.1"
                     /translation="MRARSALFTLFGDVVRPHGGEAWLSTLTAYMDALGFTAEATRTA
                     LHRMSAEGWVEPRRAGRFAAYRLTARGEARLEEAAERIYRLRAADWDKHWRLLVHRGA
                     LPAAVVRELGWVGFGRLTPDVWVSPHPHDAHLDELLAAAGLLEAVHRFTSAAGGDADR
                     IVAEAWDLSRLRAGHEEFLRQWARAKPPRDPRRAFAQRVRLVHHWRGFLFSDPGLPDA
                     LLPDDWRGEQATAVFQQRHAELRERSEPWAIARREGGPGR"
     gene            1277..2251
                     /gene="paaA"
                     /locus_tag="VNU01_13220"
     CDS             1277..2251
                     /gene="paaA"
                     /locus_tag="VNU01_13220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HWH33624.1"
                     /translation="MTAIMSPFDVDARTAAFSAHVRAGGTVEPGDWMPEAYRAGARKF
                     IEMHANSEIMGALPEREWISRAPTLRTKLSLAAKVQDEVGHGHLLYRLAEELGKPRAA
                     MVSDLLAGRSKFHNVFHYPTRTWGDVAVIGWLVDGAALVTQRALLASSYAPYVRVLRR
                     ICAEESLHQRHGEHLVLELCGGTPAQRELFTEAVARWWQPILQFFGPPSPEDADPGDE
                     AAATARQEARDPLLWWRFKTRTNESLRQEFLSLYVPKLWDLGVEPPDPDLRWDAETGR
                     WVTGPVDWSELERIGRNEGPMSQTRLAWRNWMHDEHRWVRELLREVAA"
     gene            2248..2565
                     /locus_tag="VNU01_13225"
     CDS             2248..2565
                     /locus_tag="VNU01_13225"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33625.1"
                     /translation="MSVRWMVLGRRTWEDTLVEVGEVHAPDADLALLLARDAFFRHGE
                     GVDYAVVRADAVHPCPDPSALEFATDKSYKLQAGYTGLGAKRRRVAELARSLGLVRDR
                     ARP"
     gene            2562..>2742
                     /locus_tag="VNU01_13230"
     CDS             2562..>2742
                     /locus_tag="VNU01_13230"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HWH33626.1"
                     /translation="MNARVELLLALADDALVLGHRHVQWTGVAPHLEADLAFTSIGLD
                     AMGHALVWYRLAADAD"
BASE COUNT          343 a         1017 c         1033 g          349 t
ORIGIN      
        1 caccaaccag gcgggcgagg acaaccgcaa cgtcgcgcgc atgagcctgc tgctggccgg
       61 cttccccgtc accgtgccgg gcgccctgca ccacggcctc caggccggag gcgcacaacc
      121 ggttcacggt ggcgcccggc acggtgacgg ggaagccggc cagcagcagg ctcatgcgcg
      181 cgacgttgcg gttgtcctcg cccgcctggt tggtgtcccc ggcgatgacc tcgtccacct
      241 cggccgggtc cagcccggcg cggtcgacca gggtgcgcag cgtgagcgcc agcaggtcgt
      301 ccgggcgcac gccgcgcagc gccccgccga tcctgccgac cggggtgcgc accgcctcga
      361 ccaggaacac ctcgcgcatc gccgcctcct tgtgccgcgc cgatggtagc ggcggccttg
      421 ccggccggac gcatcgtgtt acacactggc gggtagaacg tctacgaccg tcacacgtcg
      481 tccgcccgtc acgaggcccg cccatgcgcg cacgctcggc gttgttcacc ctcttcggcg
      541 acgtggtgcg cccgcacggc ggcgaggcgt ggctgtccac gctgaccgcg tacatggacg
      601 cgctcggctt cacggccgag gcgacgcgca cggccctgca ccgcatgagc gcggaaggct
      661 gggtggaacc gcggcgggcc ggccgcttcg ccgcctaccg cctcacggcg cgcggcgagg
      721 cgcggctgga ggaggccgcc gagcgcatct accgcctgcg cgccgccgac tgggacaagc
      781 actggcggct gctggtccac cgcggcgcgc tgcccgccgc cgtggtgcgc gaactcggct
      841 gggtcggctt cggccggctc acgccggacg tgtgggtgag cccgcacccc cacgacgcgc
      901 atctggacga gctgctggcc gccgcgggcc tgctggaggc ggtgcaccgc ttcaccagcg
      961 ccgccggcgg cgacgccgac cgcatcgtcg ccgaggcgtg ggacctgtcc cggctgcggg
     1021 ccggccacga ggagttcctg cggcagtggg cgcgcgccaa gccgccgcgc gacccgcgcc
     1081 gggcgttcgc gcagcgcgtc cgcctggtgc accactggcg cggcttcctg ttctccgacc
     1141 cgggcctgcc ggacgcgctg ctgccggacg actggcgcgg ggagcaggcc accgcggtgt
     1201 tccagcagcg ccacgccgag ctgcgcgagc gctcggagcc gtgggcgatc gcgcgccgcg
     1261 agggaggacc gggccggtga ccgcgatcat gagcccgttc gacgtcgacg cgcgcaccgc
     1321 ggcgttcagc gcccatgtcc gtgccggcgg caccgtggag cccggcgact ggatgcccga
     1381 ggcctaccgc gccggggcgc gcaagttcat cgagatgcac gcgaactccg agatcatggg
     1441 cgcgctgccg gagcgcgagt ggatctcgcg cgccccgacg ctgcgcacca agctgtcgct
     1501 ggccgcgaag gtgcaggacg aggtcggcca cggccacctg ctgtaccggc tggccgagga
     1561 gctgggcaag ccgcgcgcgg cgatggtctc cgacctcctg gccggtcgca gcaagttcca
     1621 caacgtcttc cactacccca cccggacctg gggcgacgtg gcggtgatcg gctggctggt
     1681 ggacggcgcc gcgctggtga cgcagcgcgc cctgctcgcc tccagctacg cgccgtacgt
     1741 gcgcgtgctt cggcgcatct gtgcggagga gagcctgcac cagcgccacg gggagcacct
     1801 ggtcctggag ctgtgcggtg gcacgccggc gcaacgcgag ctgttcaccg aggcggtggc
     1861 gcgctggtgg cagccgatcc tgcagttctt cggcccgccg tcgccggagg acgccgaccc
     1921 gggcgacgag gccgccgcga cggcacgcca ggaggcacgc gacccgctgc tgtggtggcg
     1981 cttcaagacg cgcacgaacg agtcgctgcg ccaggagttc ctgtccctgt acgtgccgaa
     2041 gctgtgggac ctgggcgtgg agccgcccga ccccgacctg cgctgggacg ccgagaccgg
     2101 ccgctgggtc accgggccgg tcgactggag cgagctggag cgcatcggcc gcaacgaggg
     2161 accgatgtcg cagacccggc tggcgtggcg gaactggatg cacgacgagc accgctgggt
     2221 gcgcgagctg ctgcgcgagg tggcggcatg agcgtgcgct ggatggtgct gggccgccgc
     2281 acctgggagg acaccctggt ggaggtcggc gaggtgcacg cgcccgacgc ggacctggcg
     2341 ctgctgctgg cacgcgacgc gttcttccgc cacggcgagg gcgtggacta cgcggtcgtg
     2401 cgggccgacg cggtgcaccc gtgccccgac ccctccgccc tggagttcgc caccgacaag
     2461 tcctacaagc tgcaggcggg ctacaccggc ctgggcgcca agcgccgccg cgtcgcggag
     2521 ctcgcgcgct cgctgggact ggtgcgcgat cgggcgcggc cgtgaacgcg cgcgtggagc
     2581 tgctgctcgc cctggccgac gacgcgctgg tgctggggca ccgccacgtg cagtggaccg
     2641 gcgtggcccc gcacctggag gccgacctcg cgttcacctc catcggcctg gacgcgatgg
     2701 gccacgcgct ggtgtggtac cgcctggccg ccgacgccga cg
//
LOCUS       DATMKC010000457         8115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_84277, whole genome shotgun sequence.
ACCESSION   DATMKC010000457 DATMKC010000000
VERSION     DATMKC010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84277"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            581..1405
                     /locus_tag="VNU01_13235"
     CDS             581..1405
                     /locus_tag="VNU01_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33627.1"
                     /translation="MARADLHYALAVDREILEAASVDPSLLDPVVRVPSGLPGEARPF
                     VVARDYQGPVGYYTEHFEIADAKGRVVHRSALRRVNLRGEMFEDRVTDVVTGLAFEDP
                     DEHQLRCFIDGEQIGQVPVFLEMGLGGDPRVAAEETFKKAIGKGEIIWITVPQAPAGR
                     RGERKTHTQPVWFITQGPKIYVLTGPGEQDVRGLVQAGEVEITARSKDLRSRVSRVPA
                     TVRVLPKDDPEWDKLAQSATPKRLNLPDKTFEDAVRRWRETCELVELTPQFGAKAA"
     gene            complement(1412..2128)
                     /locus_tag="VNU01_13240"
     CDS             complement(1412..2128)
                     /locus_tag="VNU01_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33628.1"
                     /translation="MRRRHRCWASGLLLAVLALAVGGCRLDAAIDVDLDRDGGGTLAV
                     ELVADRALVTRAAASGTDPLRDLARRVDELAGWDAQAREGPEGGRRVRLSAGFAGPDE
                     FDRLVAGFTAALDAPELRPLESLSLELDQGVVRVRGAAALRPGPAVSELGYTPEQAVA
                     ALADAVTYQVRLRVPGQVRDTNADRSTDGELIWTVPAGGSVDVWAEADLPGGAMVPLL
                     VGGAAAAGAAALWLRRRAAA"
     gene            2214..4511
                     /locus_tag="VNU01_13245"
     CDS             2214..4511
                     /locus_tag="VNU01_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWH33629.1"
                     /translation="MTEAAEPQTIPDRRRLGELLLTAGVLSRDDLDRALQSARTGPDG
                     RRERLGRTVVRLGLASEQDVARALAEQLGLAHFGGDILPMDEAVAHVIPAAIAQRHDV
                     VATGRDPDGTLVVAFADPTNIVALDDVRLATGVRRIRRIVASRQAVQAAMRKVYGFDQ
                     QSAVELLDSLETVTDEPSDIELLRAVEDAPVVRLADGILSGALQAGASDVHVEPGVAG
                     TSVRYRIDGVLRPVMTVPRAATPALVSRLKLMAGMDIAERRRPQDGRSRLRGQGEDVD
                     LRVATLPSLYGETVVMRLLRKGDERLTLSELGLSPEQLAAVQSAIERPQGLVLLTGPT
                     GSGKTSTLYAFLEHLRNDTRKIITLEDPVEYQLDGISQTQISDQIGLTFARALRTVLR
                     QDPDIVMVGEVRDPETAELALQASLTGHLVFSTLHTNDAAGAIVRLRDLGIPPYLIAS
                     SLTLVVAQRLARTICPACAAPRPPTDWQMGELHLAPQEVQRAGFAAGGGCAACGHSGY
                     RGRGGVFEVLVVDATVRRLVAEGASEPAIRRAARSAGMRSLREDGVRRAMAGETTLEE
                     VLRVTPSEGGVEGVCPVCAQEVEPDFHRCPWCSADLRPNDCVTCGRQLARGWVVCPEC
                     GTPRNPVGLDAERLPKLLVVDDDKSVRAAITAMLTGDYEVVEAADGEEALRLVHSAAP
                     DAVLCDVSMPGMDGYTVTRELRSRPVTMHLPVVLMTGQDDRDTEIEGLRSGADDHLSK
                     PLDPEVLLARLEAVLRRRTRTAGDL"
     gene            4580..5692
                     /locus_tag="VNU01_13250"
     CDS             4580..5692
                     /locus_tag="VNU01_13250"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWH33630.1"
                     /translation="MPTADQVLQALAHAEEPTLGAPVTRLELINDLVVDGGTVRIQVL
                     EPIPGAASEALGEAVRAAVRAVEGVDAVEVKVSPMPEDRRQALAAKLRGNAPAAAGGQ
                     PVIPFSQMDNETKVVAIASGKGGVGKSSITTNLAVALAQAGHKVGVLDADVWGYSIPR
                     MLGVSGQPLPFGNMVVPLRAYGCKVISIGFFIDAERPVVWRGPMLHRALQQFLADVYW
                     GDLDFLLCDLPPGTGDVAISLAQMLPNADMLVITTPQPAAQRVALRAGQVTAQTGMRV
                     AGVIENMSYFIAPDTGNTYRIFGEGGGEILAKELDTELLGAIPLDERLREGADEGVPL
                     IVGDPDAPASLVLREIAEKVARRKTSMVGRSLPLMG"
     gene            5701..6447
                     /locus_tag="VNU01_13255"
     CDS             5701..6447
                     /locus_tag="VNU01_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWH33631.1"
                     /translation="MRPLVVVPTYNEIATLERVVSGALAADARLHVLVVDDGSPDGTG
                     DLAEKLALAGDGRVAVLHRRAKSGLGSAYKRGFEWGMARGYDVLCEMDADLSHNPADL
                     ARLLAALESADLAIGSRYVRGGGVVDWPLHRRLLSRMGNRYVRLITGMPLRDATAGFR
                     AYRVPVVEEVNLDSLRAEGYAFQLEMALRTWRLGFAITEVPITFVERTEGASKISRAI
                     VVEALWRAALWGLRGPRRAAPTHPRSICSQ"
     gene            complement(6478..7116)
                     /locus_tag="VNU01_13260"
     CDS             complement(6478..7116)
                     /locus_tag="VNU01_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWH33632.1"
                     /translation="MSRKRTDWVGRALIGGALLGGSWLALRRPEAQRVDVRIGDALRH
                     AGSDGLDKAVTHTTDLGSVYAVAGVAGVLAATGRRRMAGDVLGAGLAAWSLGQGAKTR
                     VKRARPYEADGVRRLVRPPTGSSFPSGHAAVGTAMATVLADRARGPAAAGLFDLLGAY
                     VALSRVYVGVHYPTDVIGGAGLGLLIGSVWRGPVAKAGRRLVSLPLRRLRSL"
     gene            complement(7113..7463)
                     /locus_tag="VNU01_13265"
     CDS             complement(7113..7463)
                     /locus_tag="VNU01_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWH33633.1"
                     /translation="MGSIGFGELAFLVVLALLVFGPDKLPGIANTVGKALGAVRREAS
                     GTLDELKRAAELEEFRGVAPELRSIGQELRGTRDELRRGLSSGGAAMASSAAGARQQQ
                     AALSGPAPFDPDAT"
     gene            complement(7495..>8115)
                     /locus_tag="VNU01_13270"
     CDS             complement(7495..>8115)
                     /locus_tag="VNU01_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWH33634.1"
                     /translation="QVGELAVAVGSPFGLESTVTAGVISALNRPIQVTGAGGRPVVLP
                     NVIQTDAAINPGNSGGALVNGNAELIGINSAILTAGTAANAGVGFAIPVNTVVDIADE
                     LIDHGFVEHAYLGVAGLNVTPEVAERLGVDGGAYLQTVEPGGPAAEAGLRAGDVIVGF
                     AGQDIGSMDELVIAVRNADVGQTVEVTYIRDGERATTRATLTEKPR"
BASE COUNT         1101 a         3079 c         2966 g          969 t
ORIGIN      
        1 ctggcgctgc cacagccaca tccaccaaca cggctggcaa gtccggcaag aacacggccg
       61 ctacaccgcc gggccaccag gccaagcacc acccgcacca ccggaaccac tgcaccttga
      121 caacaacata gagaacccag cgctcgcgct cgcgcatacc ggctgacccg ccaccccacc
      181 cgccaggtgg ggcgcgaagc ccgcccacac cgccaacagg cgggcggggc ggcggtcggc
      241 gtgggaaccg gagaccgacg aggacgagcc cggctcgcgg cgccacacac gcgcggccgc
      301 aacctggccg ccgacgcccg acaccgcgat atcagccggg cgaggagcga ggaggggtcc
      361 gggcccacgc gaccgcggac acgcaccgcg gcatcagccg ggcgaggagc gaggaggggt
      421 ccgggcccac gcgaccgcgg acacgcgcga ccagcgaccg cagggcgtcg gcgggtgcgg
      481 atatactccc gcccggtcga tcccgatgtc ccgcctcctc cggtgccgcc gtgccggcgc
      541 cgtccggggg gcccgctgcc ccaaggagtg acgagccagc atggcgcgcg ccgacctgca
      601 ctacgcgctc gcggttgacc gcgagatcct cgaggccgcc tcggtcgatc cgtcgctgct
      661 ggaccccgtc gtccgggtgc ccagcggcct gccgggcgag gcccgcccgt tcgtggtggc
      721 gcgcgactac cagggcccgg tcggctacta caccgagcac ttcgagatcg ccgacgccaa
      781 gggccgcgtg gtgcaccgct cggcgctgcg ccgggtgaac ctgcgcgggg agatgttcga
      841 ggaccgcgtc accgacgtcg tcaccggcct ggccttcgag gacccggacg agcaccaact
      901 gcgctgcttc atcgacggcg agcagatcgg ccaggtgccg gtgttcctgg aaatggggct
      961 gggcggagac ccgcgcgtgg ccgccgagga gaccttcaag aaggccatcg gcaagggcga
     1021 gatcatctgg atcaccgtgc cgcaggcgcc ggccggccgc cgcggcgagc gcaagaccca
     1081 cacgcagccg gtgtggttca tcacccaggg ccccaagatc tacgtgctca ccggcccggg
     1141 cgagcaggac gtgcggggtc tggtccaggc cggcgaggtg gagatcaccg cgcggtccaa
     1201 ggacctgcgc agccgcgtgt cgcgcgtgcc ggccaccgtc cgcgtgctgc cgaaggacga
     1261 ccccgagtgg gacaagctgg cccagtcggc cacgcccaag cggctgaacc tgccggacaa
     1321 gaccttcgag gacgcggtgc gccgctggcg cgagacctgc gagctggtcg agctcacacc
     1381 gcagttcggc gcgaaggcgg cctagccgcc ctcaggcggc cgcgcgccgc cgcagccaca
     1441 gcgcggcggc acccgccgcc gccgccccac ccaccagcag cgggaccatc gcgcctcccg
     1501 gcaggtccgc ttccgcccag acgtccacgc ttccgcccgc cggcacggtc cagatcagct
     1561 cgccgtccgt ggagcggtcc gcgttggtgt cccgcacctg gcccggcacg cgcagccgca
     1621 cctggtaggt caccgcgtcg gccagcgcgg cgacggcctg ctcgggtgtg tagcccagct
     1681 ccgagaccgc cgggccgggc cgcagcgcgg cggccccgcg cacgcgcacg acgccctggt
     1741 ccagctcaag tgacagcgac tccagcggcc gcagctccgg cgcgtccagc gccgcggtga
     1801 agccggccac gagccgatcg aactcgtcgg ggcccgcgaa gccggccgac agccgcaccc
     1861 ggcggccacc ctccgggcct tcgcgcgcct gcgcgtccca gcccgcaagt tcgtccacgc
     1921 gcctggccag gtcgcgcaac gggtcggtcc ccgacgccgc ggcgcgtgtc accagggcac
     1981 ggtccgccac cagctccacg gccagcgtgc cgccgccgtc ccggtccagg tccacgtcga
     2041 tcgcggcgtc caggcggcac ccgcccacag ccagcgcgag gaccgcgagc agcagcccgg
     2101 acgcccagca gcggtgccgg cggcgcacgc gtcgcgtccc tttctcatcc gacgagggcc
     2161 ccgcgcgaag gcgccgaggc tacgatgcgc cgagcgtacc gaacaggatg ggcatgaccg
     2221 aggcggcaga accgcagacc atccccgacc ggcgcaggct gggcgagctg ttgctcaccg
     2281 ccggcgtgct gagccgggac gacctcgacc gcgcgctgca gtccgcgcgc accggcccgg
     2341 acgggcggcg cgagcggctc gggcggaccg tggtgcgcct tggcctggcg tcggagcagg
     2401 acgtggcccg cgcgctggcc gagcagcttg gcctggccca cttcggcggc gacatcctcc
     2461 cgatggacga ggccgtggcg cacgtcatcc ccgcggccat cgcccagcgc cacgacgtcg
     2521 tcgcgaccgg ccgggatccc gacggcacgc tcgtcgtggc gttcgccgac cccaccaaca
     2581 tcgtcgcgct cgacgacgtg cggctggcca ccggcgtccg gcgcatccgc cgcatcgtgg
     2641 cctcgcgcca ggccgtgcag gcggcgatgc gcaaggtcta cggcttcgac cagcagtccg
     2701 ccgtggagct gctggacagc ctggagaccg tcaccgacga gcccagcgac atcgagctgc
     2761 tgcgcgcggt ggaggacgcg ccggtggtgc gcctggccga cggcatcctc agcggcgcgc
     2821 tgcaggccgg cgcgtcggac gtccacgtcg agccgggcgt cgcgggaacc tcggtccgct
     2881 accgcatcga cggcgtgctg cggccggtca tgaccgtgcc gcgcgccgcc acgccggcgc
     2941 tggtgtcgcg cctgaagctc atggccggca tggacatcgc cgagcgccgc cgcccgcagg
     3001 acggtcgcag ccgcctgcgc ggccagggcg aggacgtcga cctccgcgtg gccacgctgc
     3061 cgtcgctgta cggcgagacc gtggtgatgc gcctgctgcg caagggcgac gagcggctga
     3121 cgctgagcga gctcggcctg tcccccgagc agctcgccgc cgtgcagtcg gcgatcgagc
     3181 gcccccaggg gctggtgctg ctcaccggcc cgacgggttc cggcaagacc tccacgctgt
     3241 acgcgttcct ggagcacctg cgcaacgaca cccgcaagat catcaccctg gaggacccgg
     3301 tcgagtacca gctcgacggg atcagccaga cccagatcag cgaccagatc ggcctgacgt
     3361 tcgcgcgggc gctgcgcacg gtgctgcgcc aggacccgga catcgtcatg gtcggcgagg
     3421 tccgcgaccc cgagacggcg gagctggcct tgcaggccag cctcaccggg cacctggtgt
     3481 tctcgacgct gcacaccaac gacgcggccg gcgccatcgt gcgcctgcgg gacctgggca
     3541 tcccgcccta cctgatcgcc agctccctca cgctggtggt ggcgcagcgc ctggcacgca
     3601 ccatctgccc cgcctgcgcg gccccgcgcc ccccgacgga ttggcagatg ggcgagctgc
     3661 acctggcgcc gcaggaggtg cagcgggccg gcttcgcggc cggcggcggc tgcgcggcct
     3721 gcgggcacag cggctaccgc ggccgcggcg gggtgttcga ggtgctcgtc gtcgacgcga
     3781 cggtgcgccg cctggtcgcc gagggcgcca gcgagcccgc gatccggcgg gccgcccgtt
     3841 ccgcgggcat gcgcagcctg cgcgaggacg gcgtccgccg cgcgatggcc ggtgagacca
     3901 cgctggagga agtgctgcgc gtcacgccgt cggaaggcgg cgtcgagggc gtgtgcccgg
     3961 tctgcgcgca ggaagtggag cccgacttcc accgctgccc gtggtgcagc gccgacttgc
     4021 gccccaacga ctgcgtcacg tgcgggcgcc agctcgcgcg cggctgggtg gtctgcccgg
     4081 agtgcggcac gccgcgcaac cccgtcggcc tcgacgccga gcggctcccc aagctgctgg
     4141 tggtcgacga cgacaagtcg gtacgcgccg cgatcaccgc catgctcacc ggggactacg
     4201 aggtggtgga ggcggccgac ggcgaagagg ccctgcggct ggtgcacagc gccgcgccgg
     4261 acgccgtgct gtgcgacgtc agcatgccgg gcatggacgg ctacacggtg acgcgggagc
     4321 tgcgctcccg gcccgtgacc atgcacctgc cggtggtgct catgaccggc caggacgacc
     4381 gcgacacgga gatcgaaggc ctgcgctccg gcgccgacga ccacctgtcc aagccgctgg
     4441 accccgaggt gctgctcgct cgcctggagg ccgtgctgcg ccggcgcacc cgcacggccg
     4501 gcgacctgta gccgaggcgg gttcgtcgtc gtgcgtcgtg ccgcctagca ttcagcgcac
     4561 gacgaccaag gagacccctg tgccaaccgc tgaccaggtg ctgcaggcgc tggcccacgc
     4621 cgaggagccg acgctgggcg cgcccgtcac caggctcgag ctgatcaacg acctggtggt
     4681 ggacggcggg accgtgcgca tccaggtgct ggagcccatc cccggtgcgg ccagcgaggc
     4741 gctcggcgag gccgtgcgcg ccgcggtgcg cgccgtcgag ggcgtcgacg cggtggaggt
     4801 gaaggtctcg ccgatgccgg aggaccgccg ccaggcgctc gcggccaagc tgcgcggcaa
     4861 cgcgcccgcg gccgccggcg ggcagccggt gatcccgttc agccagatgg acaacgagac
     4921 caaggtcgtg gcgatcgcct ccggcaaggg cggcgtcggc aagagttcca tcaccaccaa
     4981 cctcgccgtg gcgctggcgc aggccggcca caaggtcggc gtgctggacg ccgacgtgtg
     5041 gggctactcc atcccgcgca tgctgggggt gtccggccag ccgctgccct tcggcaacat
     5101 ggtggtgccg ctgcgcgcct acggctgcaa ggtgatctcc atcggcttct tcatcgacgc
     5161 cgagcgcccc gtggtgtggc gcgggcccat gctgcaccgg gcgctgcagc agttcctggc
     5221 cgacgtgtac tggggcgacc tggacttcct gctgtgcgac ctgccgccgg gcaccggcga
     5281 tgtcgcgatc tcgctggcgc agatgctgcc caacgccgac atgctggtga tcaccacgcc
     5341 gcagcccgcg gcccagcgcg tggcgctgcg ggcgggccag gtcacggcgc agacgggcat
     5401 gcgcgtggcc ggggtgatcg agaacatgag ctacttcatc gcgccggaca ccggcaacac
     5461 ctaccggatc ttcggcgagg gcggcggcga gatcctggcc aaggagctgg acaccgagct
     5521 gctcggtgcg atccccctgg acgagcgcct gcgtgagggc gccgacgagg gcgtcccgct
     5581 gatcgtgggc gacccggacg cgccggccag cttggtgctg cgcgagatcg ccgagaaggt
     5641 ggcgcggcgc aagacgtcga tggtcgggcg cagcctgccc ctgatgggct gagccgtccg
     5701 gtgcggccgc tcgtcgtcgt ccccacctac aacgagatcg ccacgctgga gcgggtggtc
     5761 tccggcgccc tggccgcgga cgccaggctg cacgtgctgg tggtcgacga cggctccccc
     5821 gacggcacgg gcgacctggc cgagaagctg gccctggccg gcgacggacg cgtcgccgtg
     5881 ctgcaccgcc gcgccaagtc cggcctcggc tcggcctaca agcgcggctt cgagtggggc
     5941 atggcgcgcg gctacgacgt gctgtgcgag atggacgcgg acctgtcgca caacccggcc
     6001 gatctcgcgc gcctgctcgc cgcgctggag tccgccgacc tggcgatcgg ctcgcgctac
     6061 gtgcgcggcg gcggcgtggt ggactggccg ctgcaccggc ggctgctgtc gcgcatgggc
     6121 aaccgctacg tgcgcctgat cacgggcatg ccgctgcgcg acgcgaccgc cggcttccgc
     6181 gcctatcgcg tcccggtcgt ggaggaggtc aacctcgaca gcctgcgcgc cgagggctac
     6241 gcgttccagc tggagatggc gctgcgcacg tggcggctgg gcttcgcgat caccgaggtg
     6301 ccgatcacgt tcgtggagcg caccgagggc gcgagcaaga tcagccgcgc catcgtggtg
     6361 gaggcgctgt ggcgcgcggc gctgtggggc ctgcgcggcc cgcgccgcgc cgcccccacc
     6421 cacccccgct ccatctgctc acagtgagcg gtttccccga aatacggggg caacggttca
     6481 cagtgagcgc aggcggcgca gcggcaggct gacgaggcgg cggccggcct tggcgaccgg
     6541 gccgcgccac accgagccga tcagcaggcc caggcccgcg ccgccgatga catcggtcgg
     6601 gtagtgcacc ccgacgtaga ccctgctcag cgccacgtag gcccccagca ggtcgaacag
     6661 ccccgcggcg gccgggccgc gcgcgcggtc ggcgagcacg gtggccatgg cggtgcccac
     6721 ggcggcgtgg ccggacggga acgacgagcc ggtcggcggg cggaccaggc ggcgcacgcc
     6781 gtccgcctcg tacggacgcg cccgcttcac gcgcgtcttg gcgccctgcc ccaggctcca
     6841 tgccgccagg cccgcgccga gcacgtcgcc ggccatgcga cggcgccccg tcgcggccag
     6901 gacgccggcg acgccggcga ccgcgtacac cgagccgagg tccgtggtgt gcgtcaccgc
     6961 cttgtccagg ccgtccgagc cggcgtggcg cagcgcgtcg ccgatgcgca cgtcgacacg
     7021 ctgggcctcg gggcggcgca gcgccagcca ggagccgccc agcagcgcgc cgccgatcag
     7081 cgcccgcccg acccagtcag tgcgcttccg gctcacgtcg cgtcggggtc gaagggggcc
     7141 gggccgctca gcgccgcctg ctgctgccgc gccccggccg ccgagctcgc catggcggcg
     7201 ccgccgctgg acaggccccg gcgcagctcg tcgcgggtgc cgcgcagctc ctgcccgatg
     7261 ctgcgcagct ctggcgccac gccgcggaac tcctcgagct cggcggcgcg cttcagctcg
     7321 tccagcgtgc cggaggcctc ccggcgcacg gcgccgaggg ccttgccgac ggtgttggcg
     7381 atcccgggca gcttgtcggg gccgaagacc aggagcgcga gcaccacgag gaacgccagt
     7441 tccccgaagc cgatcgagcc cacggcctgc tcctcccggc gcgcccgcgg cgcgtcaacg
     7501 cggtttctcc gtgagcgtgg cccgcgtggt ggcccgctca ccgtcacgaa tgtacgtgac
     7561 ctcgacggtc tggccgacat cggcgttgcg cacggcgatc accagctcgt ccatggagcc
     7621 gatgtcctgc ccggcgaacc ccacgatgac gtcgccggcg cgcaggccgg cctcggcggc
     7681 gggaccgccg ggctccacgg tctgcaggta ggcgccgccg tcgacgccca ggcgctcggc
     7741 gacctccggg gtgacgttca gcccggcgac acccaggtag gcgtgctcca cgaagccgtg
     7801 gtcgatcagc tcgtcggcga tgtcgaccac cgtgttcacc gggatcgcga agccgacccc
     7861 ggcattggcc gcggtgccgg cggtgaggat cgccgagttg atgccgatga gctcggcgtt
     7921 gccgttgacc agcgcgccgc ccgagttgcc cgggttgatc gccgcgtcgg tctggatcac
     7981 gttgggcagc accaccggcc ggcccccggc gccggtgacc tggatcgggc ggttcagcgc
     8041 gctgatgacg ccggccgtca cggtgctctc cagcccgaag gggctgccga cggcgaccgc
     8101 gagctcgccc acctg
//
LOCUS       DATMKC010000458         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_107821, whole genome shotgun sequence.
ACCESSION   DATMKC010000458 DATMKC010000000
VERSION     DATMKC010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107821"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..839
                     /locus_tag="VNU01_13275"
     CDS             285..839
                     /locus_tag="VNU01_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33635.1"
                     /translation="MMNSDPFEELARDPGLRWADERYRAELRAEAEEYERLAAKDLLR
                     GRNLQDVVRELCHRGDIAALVLDSRTLVGEIVYAAGDLARVRTLNGDIDVNLRGVTAL
                     RVVERSLAGGRSCLRGPTSFTARLFEHESAGDRVELSTRMPTGEVIGRIHAVAIDHVV
                     VTDDDGQFWYVPLSAIDCVADCPI"
     gene            complement(851..1075)
                     /locus_tag="VNU01_13280"
     CDS             complement(851..1075)
                     /locus_tag="VNU01_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWH33636.1"
                     /translation="MVLGSDDYPPVLDAGQVARLLGLNIDYVRRLSREGVIPAHRIPG
                     GRSFRYLKDEIIEWLRAQPATMEEREDAGA"
     gene            complement(1132..2736)
                     /locus_tag="VNU01_13285"
     CDS             complement(1132..2736)
                     /locus_tag="VNU01_13285"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWH33637.1"
                     /translation="MTRRVRLRRWQKEALERLHATAPRDFLVTATPGAGKTTFGLVAV
                     LHFLREHPGARLVVVTPSRSLKAQWAMAAAGVGLELVSEWDGGRLPPDAHGVVVTYAQ
                     VGSRPREVAALARGGAALIDEIHHAGAERTWGDGLAVALEDALLRVALSGTPWRRDAN
                     PIPFVRYTDDVAQPDFAYGYARALEDGGVVRPVFFPRLGGSMEWVANDGAGMSATFDD
                     ALDARGSSQRLRTALAPDGGWLRAAVAQAHERLAGVRADDPRAGGIVFAMDQDHARGV
                     AKVVHDITGRAPVLAISDEPDAHHRLARFAHSDEPWAVCVRYASEGYDAPRLRVGVYA
                     TNVVAPLFWAQAVGRVVRWRPESGPRQAAWMFIPDDARLRELALSVAEQRTHVLRRRE
                     DDPDEDERQARLREPGDQLSLFAAVHSEADGIRGDLAVADPWRVPVGAARDADDPALE
                     IELPPPPRVVSPGDGPDGVQTVDRGVLRALNSERVTMIARLTGLGHREVNARLNKVSG
                     ILRIGEATRGQLQRRLAEADRWLTRL"
     gene            complement(3021..>3406)
                     /locus_tag="VNU01_13290"
     CDS             complement(3021..>3406)
                     /locus_tag="VNU01_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33638.1"
                     /translation="GAMIAATGVLGLSAVLAGDANVRFGDTPGARHGTVSVIDTAGPE
                     DAAWPSDLQASADGDTAHPGDRALLERAMPFVAVDEREHDRRQQHARRESPYYFEAGD
                     AWVALDPEGDLRKTQSLVHDVVAGR"
BASE COUNT          464 a         1231 c         1258 g          453 t
ORIGIN      
        1 gctccatggg cggcggcggc atgcacgagg ccgccgccgg gcccgcccac agcaccatga
       61 ccgccaacgc cgtggccgtc gccgccatcg ccttcatccc gcgcccgttc cgcatgggga
      121 tctcctcctt cagcttgtcc cacaccggtg atcttgtcct gtgcgtggga cgaccaccgc
      181 cgccccaggg ttcccgtgcc ggaacgaccg ttcacgaaat gtgtggacgc attgatcggc
      241 ggacgcgcac actggacgag gagcattccc gggggagcgg caacatgatg aattccgacc
      301 cgttcgagga attggcgcgg gatccggggc tgcggtgggc ggatgagcgg taccgggcgg
      361 agttgcgggc cgaggcggag gagtacgagc ggctggccgc caaggacctg ctgcgtggcc
      421 ggaacctgca ggacgtcgtc cgtgagctgt gtcaccgcgg cgacatcgcg gcgctggtgc
      481 tggacagccg gacgctggtg ggcgagatcg tctacgccgc cggcgacctg gcacgcgtgc
      541 ggacgctcaa cggcgacatc gacgtgaacc ttcgcggcgt gacggcgctg cgcgtggtgg
      601 agcgctcgct ggcgggcggc cgttcctgtc tcagagggcc gaccagcttc acggcgcggc
      661 tgttcgagca cgagtcggcg ggcgaccgcg tggagctgtc cacccgcatg ccgaccggcg
      721 aggtgatcgg ccgcatccac gcggtggcga tcgaccacgt ggtggtcacc gacgacgatg
      781 gccagttctg gtacgtgccc ctgagcgcga tcgactgtgt cgccgactgc ccgatctgag
      841 cccgctccgc tcaggctccc gcgtcttccc gctcttccat ggtggccggc tgggcacgca
      901 gccactcgat gatctcgtcc ttgaggtagc ggaagctgcg gcccccaggg atgcgatgcg
      961 cggggatcac accttcccgg gacaggcggc ggacgtagtc gatgttcagg ccgagcaagc
     1021 gcgccacctg acccgcgtcg agcacaggtg ggtagtcgtc ggagcccagg accatggggg
     1081 gatcgtacgc ctgcccgaga ttgggcgggc gcggttgtcc ccaacgtcac ctcacaggcg
     1141 cgtgagccag cggtcggcct cggccaggcg ccgctggagc tgcccccggg tggcctcacc
     1201 gatgcggagg atgcccgaga ccttgttcag gcgcgcgttg acctcccggt gccccaagcc
     1261 ggtcagccgg gcgatcatcg tgacgcgctc gctgttgagc gcccgcagca cgccccggtc
     1321 caccgtctgc acgccgtccg gaccgtcgcc gggggacacc acccgtggcg gcggcggcag
     1381 ctcgatctcc agggcggggt cgtccgcgtc gcgcgccgct ccgacgggca cccgccaggg
     1441 gtcggccacg gcgaggtcgc cgcggatccc gtccgcctcg gagtggaccg cggcgaacag
     1501 ggacagctgg tcacccggct cgcgcaggcg cgcctgacgc tcgtcctcgt cagggtcgtc
     1561 ctcacggcgc cgcagcacgt gggtgcgctg ctcggccacc gacagcgcca gctcgcgcag
     1621 gcgcgcgtcg tccgggatga acatccacgc cgcctggcgc gggccggact cggggcgcca
     1681 gcgcaccacg cggcccaccg cctgcgccca gaacagcggc gcgaccacgt tggtggcgta
     1741 gacgccgacg cgcagccgcg gggcgtcgta gccctcgctg gcgtagcgca cgcacaccgc
     1801 ccacggctcg tcgctgtgcg cgaagcgcgc caggcgatgg tgggcgtccg gctcgtcgga
     1861 gatggccagg accggggcgc ggccggtgat gtcgtgcacc accttggcga cgccgcgcgc
     1921 gtggtcctga tccatcgcga acacgatgcc gcccgcgcga gggtcgtccg cgcgtacgcc
     1981 ggccaggcgc tcgtgggcct gcgccacggc ggcgcgcagc cagccgccgt ccggggccag
     2041 cgcggtgcgc agccgctgcg aggagccgcg ggcgtcgagc gcgtcgtcga aggtggcgct
     2101 catgcccgcg ccgtcgttgg cgacccattc catggagccg cccaggcgcg ggaagaacac
     2161 cgggcggacg acgccgccgt cctccagcgc gcgggcgtag ccgtaggcga agtccggctg
     2221 ggccacgtcg tcggtgtagc gcacgaacgg gatggggttg gcgtcgcgcc gccacggggt
     2281 gcccgaaagc gccacccgca gcagcgcgtc ctcgagcgcg accgccagcc cgtcacccca
     2341 ggtgcgctcc gcccctgcgt ggtggatctc gtcgatcagc gcggcgccgc cgcgcgccag
     2401 cgccgccacc tcccgcgggc gcgagcccac ctgcgcgtag gtcaccacga cgccgtgcgc
     2461 gtccggcggc aggcggccgc cgtcccattc cgagaccagt tccaggccga cgcccgcggc
     2521 ggccatcgcc cactgcgcct tgaggctgcg cgaaggcgtg acgacgacga ggcgcgcgcc
     2581 ggggtgctca cgcaggaagt gcagcaccgc caccaggccg aacgtggtct tgccggcgcc
     2641 cggcgtggcg gtcaccagga agtcccgcgg cgcggtcgcg tggaggcgtt ccagcgcttc
     2701 cttctgccag cggcgcaggc gcacgcggcg ggtcatgcgg gcgctcccgg gcgcgatgat
     2761 gaggcggcag gcggcatgcg gagtcagtcg gtccctgggc tggcggcggg cccgccggcc
     2821 aggcctggcg ggggcggccg agtgtagagc gcaagccgga cgaatcacgc gcaccaccga
     2881 aatattgggg agccctgatg cgtcgctcat gtggggagcc gccgcgacat gcggcaaggg
     2941 aagacccccg gcgaggcgct gagcgtctcg ccgggggccg gtgacagcca gggcttgcgg
     3001 tgctggaggg aaccgctgaa tcagcggcca gccaccacgt catgtaccag cgattgcgtc
     3061 ttgcgcagat cgccctcggg gtcgagggcg acccacgcgt cgccagcctc gaagtagtac
     3121 ggcgattctc ggcgggcatg ctgctggcgc cgatcgtgct cacgctcgtc gaccgcgacg
     3181 aacggcatgg cgcgttccaa caacgcgcga tctccagggt gcgccgtgtc cccgtccgcg
     3241 gacgcctgca gatcgcttgg ccaggccgcg tcctccggac cggctgtgtc gatcaccgac
     3301 acggtcccgt gccgcgcacc cggagtgtcg ccgaatctca cgttcgcgtc tcctgccagg
     3361 accgcggaca agccgagcac cccagtggca gcgatcatgg cgcccg
//
LOCUS       DATMKC010000459         5807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_19364, whole genome shotgun sequence.
ACCESSION   DATMKC010000459 DATMKC010000000
VERSION     DATMKC010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19364"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VNU01_13295"
     CDS             <1..1165
                     /locus_tag="VNU01_13295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWH33639.1"
                     /translation="DVSRTLLVAALALALALPAVPATAQQDDEPGLTAPELTALGGVL
                     YDPAADQVLWGRREDVGIPMASTTKIMTTLLALEAGGLDETVEVSREAAQAGGAGLGL
                     RMGQRLPLRSLLAGLMLRSGNDAATAVAEHVAGTEEAFVARMNARARQLGLSDTHFVN
                     ASGLTDDREHRASPLDLARLTKAAMAHAEFARWAGAPRLTVPGLAPMENRNELVGRYP
                     GATGVKTGYLSHAGFALVASAEREELTLYAVVLGSQDSFRDAARILDWGYARYRLARP
                     AAAGAVAGRYRWAGADTPLTAVRELAAAVPRDAQVAYRLRVRPLMALPVTAGTPLGEA
                     ELVAGGEVVATTPLVAGHDVAAPPPRDAPAEAGAALQDGLRALARLHPGSAAA"
     gene            1162..1767
                     /locus_tag="VNU01_13300"
     CDS             1162..1767
                     /locus_tag="VNU01_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWH33640.1"
                     /translation="MSDAPLRLAWPRAAIRARVAELGTEITARYAGQELMLVGVLNGA
                     AVFLADLVRCLELPVRIDFLALRPVDPHAVGEAPAALLKDLELDIGGRQVLLVEDVVH
                     SGVTLAQVLRLLQARDPASLRVCTLLDRTVARVTALPVDHVGFRVGLAPLVGYGLDLG
                     GHFRALPDLWEVTDEHALLRDPAGAAESARRALTAREAARD"
     gene            1834..2304
                     /locus_tag="VNU01_13305"
     CDS             1834..2304
                     /locus_tag="VNU01_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWH33641.1"
                     /translation="MIELELVGVRVELPHNQPIVLLKERAGDRFLPIWIGAVEATSIA
                     FALQGVVTARPMTHDLMRNLLEDLKVSVERVVVTELRDGTFYAEIQMTSDGESFVTSS
                     RPSDAIALAVRATVPIYADEAVLAEAGIQIEDEEEDEVERFKEFLDQVTPEDFR"
     gene            2431..2955
                     /locus_tag="VNU01_13310"
     CDS             2431..2955
                     /locus_tag="VNU01_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWH33642.1"
                     /translation="MSSNRYLRDIPLPGLDEGPVGYRGPHVCKVVGITYRQLDYWTTT
                     ELVKPSIRDADGSGTQRLYAFEDIVQLKVIKRLLDAGISLQQIRKALDWVRERGLSLR
                     NVTLLADAKRVYAVTDEREMFDIISQGQGVFALAIDPVVQEAEAEVHALPAERAMPPV
                     ARRRKTGEAEAVNQ"
     gene            3058..4416
                     /gene="gcvPA"
                     /locus_tag="VNU01_13315"
     CDS             3058..4416
                     /gene="gcvPA"
                     /locus_tag="VNU01_13315"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWH33643.1"
                     /translation="MRFAPHTADDIRAMLDVVGKPDLDALFAHIPDALRPARPLRLDA
                     GRSEDEVLRRAGELAARNRVGAVCFAGGGAYDHVVPSVIGALLSRGELLTAYTPYQPE
                     VSQGMLQALFEYQTVIAELCGLPVSNASLYDGGSAVAEAVSMACAATRRNRVLVAQSV
                     DAPSRQVVRTYGHPLGRVVDELATDPRLGTTPVAEPGDDVAAVVIQQPNALGVIEDVR
                     AWAGAAHAAGAKLIVKLDPLAVGLLPRPGEQGADLVVGEGQPLGQGLSFGGPSFGFLA
                     CTDDQVRRLPGRVVGQTVDASGTRGYVMTLRAREQDIRREKATSNICTNQTLNAVASL
                     VYLTWLGPLGLRDLARDCLSRAHHTAALLARVDGAEVAVAGPFWREFPLRLDVPDPAE
                     AVRALHREGYLVGPVVPDGPAAGAVMVACTERRTRADAEGLASAVQRVVQGLRGQVKG
                     AA"
     gene            4416..>5807
                     /gene="gcvPB"
                     /locus_tag="VNU01_13320"
     CDS             4416..>5807
                     /gene="gcvPB"
                     /locus_tag="VNU01_13320"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWH33644.1"
                     /translation="MPVMGDRRAAEPTIFDKSRPGRRAYSLPALDVDELDPAEALPGV
                     ELTRVLPRLPEVAELDLVRHFTRLSQRNHGIDVGFYPLGSCSMKYNPRLAETAAALPG
                     FRDLHPWAPEEAAQGTLELLWDLERMLGELTGMPHATFQPAAGAHGELCGLMLIRAYH
                     SDRGDARRTIVVPDSAHGTNPASAAMCGYDVVTCPSRPDGLVDVDALEALVDEHTAGL
                     MLTNPNTLGLFEVEIARIAQVMHRVGALLYYDGANFNAICGRVRPGDMGFDVVHLNVH
                     KTFATPHGGGGPGAGPVLVSDRLAPYLPGPVVARRDDGTFGWSEPPKSIGRLHGFHGN
                     VGILVRAWTYLLAHGADGLPMISALAVLNANYVAAQVRDVLPLHSDQPPMHEFVSTGA
                     GLKRHGVRVLDLCKRLIDLGYHPPTNYFPLIVDEALMVEPTETETRETLDDFAAALRR
                     IVREAAESPELLRD"
BASE COUNT          759 a         2108 c         2184 g          756 t
ORIGIN      
        1 ggacgtgagc cgcacgctgc tggtcgcggc gctggccctg gccctggcgc tgccggccgt
       61 gcccgccacg gcgcagcagg acgacgaacc cggcctcacc gcccccgagc tcaccgcgct
      121 gggcggggtc ctctacgacc ccgccgccga tcaggtgctg tgggggcgcc gggaggacgt
      181 cggcatcccg atggcctcca ccaccaagat catgaccacg ctgctggcgc tggaggccgg
      241 cgggctggac gagacggtgg aggtgtcgcg cgaggcggcg caggccggcg gggccggcct
      301 ggggctgcgc atgggccagc ggctgccgct gcgcagcctc ctggccgggc tgatgctgcg
      361 cagcggcaac gacgcggcca ccgcggtggc cgagcacgtg gccggcaccg aggaggcgtt
      421 cgtcgcccgg atgaacgcgc gcgcccggca gctcggcctt tccgacacgc acttcgtgaa
      481 cgcctccggc ctgaccgacg accgcgagca ccgtgccagc cccctcgacc tggcgcggct
      541 gacgaaggcg gcgatggccc acgcggaatt cgcgcgctgg gcgggcgcgc cgcggctcac
      601 tgtgcccggg ttggcgccca tggagaaccg caacgagctg gtgggccgct acccgggcgc
      661 caccggcgtc aagaccggct acctgtccca cgcgggcttc gcgctggtgg ccagcgccga
      721 gcgcgaggaa ctcacgctgt acgcggtggt gctgggcagc caggacagct tccgcgacgc
      781 ggcccggatc ctggactggg gctatgcccg ctaccgcctg gcgcgcccgg cggccgcggg
      841 cgccgtcgcg ggccgctacc gctgggccgg tgcggacacg ccgctgaccg ccgtccggga
      901 gctggccgcg gcggtcccgc gcgacgccca ggtcgcctac cgcctgcgcg tgcggccgct
      961 gatggcgctg cccgtcaccg ccggcacccc gctgggcgag gcggaactgg tggccggcgg
     1021 cgaggtcgtg gccaccacgc cgctcgtcgc gggacacgac gtggcggcgc ctccgccgcg
     1081 cgacgccccg gcggaggcgg gcgccgcgct gcaggacggc ctgcgcgcgc tggcgcggct
     1141 gcacccgggg agcgcggccg cgtgagcgac gcgccgctgc ggctggcgtg gccgcgcgcc
     1201 gccatccggg cgcgcgtggc cgagctgggc acggagatca ccgcgcgcta cgccgggcag
     1261 gagctcatgc tcgtcggcgt gctcaacggc gcggcggtgt tcctggccga cctggtgcgc
     1321 tgcctggagc ttccggtccg catcgacttc ctggcgctgc gcccggtgga cccgcacgcg
     1381 gtcggtgagg cgccggccgc gctgctgaag gacctggagc tggacatcgg gggtcgccag
     1441 gtgctgctgg tcgaggacgt cgtgcacagc ggggtgacgc tggcccaggt gctgcgcctg
     1501 ctgcaggcgc gcgaccccgc gtcgctgcgc gtgtgcacgc tgctggaccg cacggtcgcg
     1561 cgcgtcaccg ccctgccggt ggaccacgtc gggttccgcg tgggcttggc gccgctggtg
     1621 ggctacgggc tggacctggg cgggcacttc cgggcgctgc cggacctttg ggaggtcacc
     1681 gacgagcacg ccctgctgcg ggacccggcc ggcgccgccg aatcggcccg gcgagctttg
     1741 acggcgcggg aagcggcgcg cgattgaggg ggtcggtgcg cccgcctatc cttggattcc
     1801 aagccgcgca ccgaggagac ctcgaggcac tcgatgatcg aactggaact tgtgggcgtt
     1861 cgcgtcgagc tgccgcacaa ccagcccatc gtcctgctca aggagcgggc cggcgaccgg
     1921 ttcctgccca tctggatcgg ggcggtggag gccacctcca tcgcgttcgc gctccagggc
     1981 gtggtcacgg cccggcccat gacgcacgac ctcatgcgca acctgctgga ggacctcaag
     2041 gtctccgtgg agcgggtggt ggtcacggag ctccgcgacg gcacgttcta cgccgagatc
     2101 cagatgacct ccgacggcga gagcttcgtc acgtcctcgc ggccctccga cgccatcgcg
     2161 ctggcggtgc gcgccaccgt gccgatctac gccgacgagg ccgtcctggc ggaagccggc
     2221 atccagatcg aggacgagga ggaggacgag gtcgagcgtt tcaaggagtt cctcgatcag
     2281 gtgacaccgg aggacttccg ctagagtcgg cggcagcgca cgggggccaa caaaccgatt
     2341 gacggccggg ggccctcgga gtaggctgtc catcggcgta attacgccaa tgcggtttct
     2401 tccccacggc cacgtcttga ggtgcgcgcg atgtcctcca accgctactt gcgggacatt
     2461 ccccttcccg gcctggacga gggcccggtc ggctaccgcg gcccgcacgt ctgcaaagtg
     2521 gtcggcatca cctaccgcca gctcgactac tggaccacca cggagctggt gaagccgtcc
     2581 atccgcgacg cggacggctc cggcacccag cggctgtacg ccttcgagga catcgtccag
     2641 ctcaaggtga tcaagcgcct gctggacgcc ggcatcagcc tgcagcagat ccgcaaggcg
     2701 ctggactggg tgcgcgagcg cgggctgtcc ctgcgcaacg tgacgctgct ggccgacgcc
     2761 aagcgcgtgt acgcggtcac cgacgagcgc gagatgttcg acatcatcag ccagggccag
     2821 ggcgtgttcg ccctggcgat cgaccccgtg gtgcaggagg ccgaggcgga ggtgcacgcc
     2881 ctgccggccg aacgcgccat gccgccggtc gcacggcgcc gcaagacggg cgaggccgag
     2941 gccgtcaacc agtagcaccg gtcacaccac gagcaagcag cggttccttc ttcccccgaa
     3001 gggcacaacc ttccgaggac cccccatgag gcccttgatg ggagaaggca ggagactgtg
     3061 cggttcgcgc cccacaccgc cgacgacatc cgcgccatgc tcgacgtcgt cggcaagccc
     3121 gacctcgacg cgctgttcgc gcacatcccc gacgcgctgc ggcccgcacg gccgctgcgc
     3181 ctggacgcgg gccgctccga ggacgaggtg ctgcgccgcg ccggggagct ggcggcgcgc
     3241 aaccgcgtcg gcgccgtgtg cttcgccggc ggcggcgcct acgaccacgt cgtgccgtcg
     3301 gtcatcgggg cgctgctgtc gcgcggcgag ctgctgaccg cgtacacccc gtaccagccc
     3361 gaggtcagcc agggcatgct gcaagccctg ttcgagtacc agacggtgat cgccgagctg
     3421 tgcgggctgc cggtgtccaa cgccagcctg tacgacggcg gcagcgcggt cgccgaggcg
     3481 gtgtccatgg cctgcgcggc cacgcggcgc aaccgcgtcc tggtcgcgca gtcggtggac
     3541 gcgccgtcgc ggcaggtggt gcgcacctac ggccacccgc tgggccgcgt ggtggacgag
     3601 ctggcgaccg acccgcgcct cggcacgacg cccgtcgccg agcccggcga cgatgtcgcc
     3661 gccgtcgtca tccagcagcc caacgcgctc ggcgtgatcg aggacgtgcg cgcgtgggcg
     3721 ggcgccgcgc acgccgcggg ggcgaagctc atcgtcaagc tggacccgct cgcggtcggg
     3781 ctgctgccgc ggccggggga gcagggcgcg gacctggtgg tcggcgaagg ccagccgctc
     3841 gggcaaggac tgtccttcgg cgggcccagc ttcggcttcc tggcgtgcac ggacgaccag
     3901 gtgcggcgcc tgcccggccg cgtcgtcggc cagaccgtcg acgcgagcgg gacgcgcggc
     3961 tacgtcatga cgctgcgggc ccgcgagcag gacatccggc gggagaaggc gaccagcaac
     4021 atctgcacca accagacgct gaacgcggtc gcctcgctgg tgtacctcac gtggctgggg
     4081 ccgctgggcc tgcgcgacct cgcgcgcgac tgcctgtcgc gcgcgcacca cacggcggcg
     4141 ctgctggcgc gggtcgacgg cgccgaggtg gccgtggccg ggccgttctg gcgcgagttc
     4201 ccgctgcggc tggacgtgcc ggacccggcc gaggcggtgc gcgcgctgca ccgggagggc
     4261 tacctcgtcg gcccggtggt gcccgacggc cccgccgcgg gggcggtcat ggtcgcctgc
     4321 accgagcggc gcacgcgcgc ggacgcggag ggattggcgt cggcggtgca acgcgtggtc
     4381 caggggctgc gcggccaggt gaagggagcg gcctgatgcc cgtcatggga gaccgccgcg
     4441 cggccgagcc caccatcttc gacaagtccc gtcccgggcg ccgggcgtac agcctgcccg
     4501 cgctcgacgt ggacgagctg gatcccgccg aggcgctgcc cggcgtggag ctgacgcgcg
     4561 tgctgccgcg gctgccggag gtggccgagc tggacctggt acgccacttc acgcgcctgt
     4621 cgcagcgaaa ccacggcatc gacgtgggct tctacccgct gggctcgtgc tccatgaagt
     4681 acaacccgcg gctggcggag acggccgcgg cgctgcccgg cttccgcgac ttgcacccgt
     4741 gggccccgga ggaggccgcg cagggcacgc tggagctgct gtgggacctg gagcgcatgc
     4801 tcggggagct cacgggcatg ccgcacgcca cgttccagcc ggcggcgggc gcgcacggcg
     4861 agctgtgcgg gctgatgctg atccgcgcct accactccga ccgcggcgac gcgcggcgca
     4921 ccatcgtggt gcccgacagc gcgcacggga ccaacccggc cagcgccgcg atgtgcggct
     4981 acgacgtggt gacctgtccc agccgcccgg acggcctggt ggacgtcgac gcgctggagg
     5041 cgctggtcga cgagcacacc gccgggctga tgctgaccaa ccccaacacg ctggggctgt
     5101 tcgaggtgga gatcgcgcgc atcgcgcagg tcatgcaccg cgtcggcgcg ctgctgtact
     5161 acgacggggc caacttcaac gcgatctgcg ggcgcgtgcg ccccggcgac atgggcttcg
     5221 acgtggtgca cctcaacgtg cacaagacgt tcgccacccc gcacggcggc ggcgggcccg
     5281 gcgccggccc cgtgctggtg tccgaccggc tggcgccgta cctgccgggc cccgtggtgg
     5341 cgcgccgaga cgacggcacg ttcggctggt cggagccgcc caagtcgatc ggccggctgc
     5401 acggcttcca cggcaacgtc ggcatcctgg tgcgcgcgtg gacgtacctg ctggcgcacg
     5461 gcgcggacgg gctgccgatg atctcggcgc tggcggtgct caacgccaac tacgtcgcgg
     5521 cgcaggtgcg cgacgtgctg ccgctgcact cggaccagcc accgatgcac gagttcgtct
     5581 ccaccggcgc ggggctcaag cgccacggcg tgcgcgtgct ggacctgtgc aagcggctga
     5641 tcgacctggg ctaccacccg ccgacgaact acttcccgct gatcgtcgac gaggcgctca
     5701 tggtggagcc cacggagacg gagacgcgcg agacgctgga cgacttcgcc gcggcgctgc
     5761 gccggatcgt ccgggaggcc gccgagtccc cggagctgct gcgggac
//
LOCUS       DATMKC010000460         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_230982, whole genome shotgun sequence.
ACCESSION   DATMKC010000460 DATMKC010000000
VERSION     DATMKC010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230982"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1270)
                     /locus_tag="VNU01_13325"
     CDS             complement(95..1270)
                     /locus_tag="VNU01_13325"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HWH33645.1"
                     /translation="MSDRPMQPDETAFPQTEEALGLLASTGLPSEARARHLHAIRLRA
                     AELGPAPAPRDAALGAGPGRARRSSEATLSGLRWRGRRAAAVAGVWLGSLSLGTGVAA
                     AAAMDDLPGDTLYGLKRAVESVQLRLPGDTQEDVRRRLELAQRRLGEAEALRARGAAD
                     ALLAGALRDAERLFDDASTLADGDADLSAEVAGSASQARDRLTQLLEEGLPEQAAERA
                     REAIESATERAGGPDGDDDGDRGRGGDRPRNAPGTMRPSESDDDGDDADGDGQGRGRG
                     RGGGDEDDDAAAPQESGRGSAPPSEAGDDRGRAPSEEGRSSDPEDDDEDEDDDERSGD
                     SQSAGDVSGQSHSGGQAGNPGPGEHQGQGQGLGRPEGSGGDSGQDRRRGSDDAGDED"
     gene            complement(1314..1925)
                     /locus_tag="VNU01_13330"
     CDS             complement(1314..1925)
                     /locus_tag="VNU01_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWH33646.1"
                     /translation="MSETLPEPSPHVAGEPVFTDALAQGVRTRDPDALAWCYEALADA
                     LFRYVRGLCGDPVLAEDLVEETFLELVEYAPALTGGLGGLRAWLFRAARNNLIDARRK
                     SARRGDVPWEDDFATQRPDSDPGPELQTLHRERGAVLETALAQLSEDQREVVLLRFLS
                     GLSGPEVAAATGRSVGAVKSLQHRGLAALARVLGTDQDLRDFP"
BASE COUNT          237 a          871 c          765 g          288 t
ORIGIN      
        1 acgcccaagc gtccagaccc ctgccgcatc ccggctcaca caggcgggac aatcccacac
       61 gtgccagcac ccggttgaca agacctacgg ccagctagtc ctcgtcgccg gcgtcgtctg
      121 acccgcgcct gcggtcctgg ccgctgtcgc cgccgctgcc ctcgggacgg ccaaggccct
      181 gcccctggcc ctgatgctcg cccgggcccg ggttccccgc ctggccgccg ctgtggctct
      241 gccccgaaac gtcgccggcg ctctggctgt cgccagagcg ctcgtcgtcg tcttcgtcct
      301 cgtcgtcgtc ctcgggatcg gaggaccgcc cctcctcaga aggggctcgt cctcggtcgt
      361 ccccggcttc ggatggcggt gcgctcccgc gtcctgactc ctggggggcg gccgcgtcgt
      421 cgtcctcgtc gcctccgcct cgtccgcgcc cgcggccctg cccgtcgccg tcggcatcgt
      481 caccgtcgtc gtcggattcg gacgggcgca tggtccccgg ggcgttgcgt ggccggtcgc
      541 caccgcggcc ccggtcgccg tcatcgtcgc cgtccggccc accggcgcgc tccgtggcgg
      601 actcgatcgc ctcacgggcc cgttcggcgg cctgctccgg cagcccctcc tcgagcagct
      661 gcgtcagccg gtcgcgcgcc tgcgacgccg atcccgcgac ctcggcgctc aggtccgcgt
      721 cgccgtcggc gagcgtgctc gcgtcgtcga acaggcgctc cgcgtcgcgc agcgcgcccg
      781 cgagcagggc gtcggccgcc ccgcgtgccc gcagcgcctc cgcctcgccc aggcgacgct
      841 gcgccagctc caggcggcgc cgcacgtctt cctgcgtgtc cccgggcagg cgcagctgca
      901 ccgactccac cgcgcgcttc aacccgtaca gcgtgtcgcc cggcaggtcg tccatggcgg
      961 ccgcggcggc cacgccggtg cccagcgaca gggatccgag ccacacgccg gccacggcgg
     1021 cggcacggcg cccgcgccag cgcaggccgg acagcgtcgc ttcggaggag cgccgcgctc
     1081 gcccgggccc ggcccccagc gcggcgtcgc ggggggcggg agccgggccc agctcggcgg
     1141 cgcgcaagcg gatcgcgtgc agatgccgcg cgcgcgcctc ggagggcagc ccggtggacg
     1201 cgagcaggcc cagcgcttcc tcggtctgag gaaacgccgt ttcgtctggc tgcatcggtc
     1261 ggtcactcat gtctgctctc cacctccttc cagcgcgcgg ggggtccgat tggttacggg
     1321 aagtccctca agtcctgatc ggtgcccagc accctcgcca gcgcggccag gccgcggtgc
     1381 tgcagcgatt tcaccgcgcc gacgctgcgc cccgtcgccg cggccacttc cgggccggac
     1441 aggccggaca ggaagcgcag cagcaccacc tcgcgctggt cctccgacag ctgcgccagc
     1501 gcggtctcca gcacggcgcc gcgctcgcgg tgcagcgtct gcagctccgg gccggggtca
     1561 ctgtccgggc gctgcgtggc gaagtcgtcc tcccagggca cgtcgccgcg gcgcgccgac
     1621 ttgcggcgcg cgtcgatgag gttgttgcgc gccgcgcgga acagccaggc gcgtagcccg
     1681 cccaggccgc cggtcagcgc cggcgcgtac tccaccagct ccaggaacgt ctcctcgacc
     1741 aggtcctcgg ccagcaccgg gtcgccgcac aggccgcgga catagcggaa cagcgcgtcc
     1801 gcgagcgcct cgtagcacca tgccagcgcg tccgggtcac gcgtgcgcac gccctgcgcc
     1861 agggcgtccg tgaagacggg ctcccccgca acgtgcgggg aaggttccgg gagggtctcg
     1921 gacattcggc ggccagcgtg gacccccgga cccctccccg caagcgatca cggggggaca
     1981 gttcgtcaag acggcccgta gactccctcg gtgcggcggc cccgcggtcg ggctgccggg
     2041 gcccggcagc ggtctgggct cgacatcgga cgggagcggc gcatgcagga cctcgacggc
     2101 ctactggccc gcaacctgga gcgcgagcgc gcggaggcgc gcgacctcgg tgcggtggtg
     2161 g
//
LOCUS       DATMKC010000461         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_11933, whole genome shotgun sequence.
ACCESSION   DATMKC010000461 DATMKC010000000
VERSION     DATMKC010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11933"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VNU01_13335"
     CDS             complement(<1..985)
                     /locus_tag="VNU01_13335"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWH33647.1"
                     /translation="MAKHILVTGGVSSSLGKGITAASLGRLLKVRGLRVTMQKLDPYV
                     NVDPGTMNPFEHGEVFVTEDGGETDLDLGHYERFIDENLHRNSSVSTGQIYSAVIAKE
                     RRGDYLGKTVQVIPHITDEIKGRILALGQDADVVITEVGGTVGDIEGLPFLEAIRQLR
                     YDVGRDNICYVHCALVPFIGPTGELKTKPAQHSVRELRSIGIQPDAVVCRADRPLSPE
                     LKRKIALLSDVDIEGVVSAHDAESLYSVPLVLREEGLDDFVVRRLGLDPAVQPELADW
                     TAMVHRQLNPSDTVRIAVVGKYVSLPDAYLSVVESLTHAGIHHGVDVEIEWV"
     gene            complement(1155..2105)
                     /locus_tag="VNU01_13340"
     CDS             complement(1155..2105)
                     /locus_tag="VNU01_13340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWH33648.1"
                     /translation="MAQDIKELGRLGRAMNAGAASAPRRRRRRFGVQRDGAFVSRRRT
                     RVPRGVVWALAAISAAVVWVGMAASRQADPRVALATIPAAPAPLDATGDVPRRLAGTD
                     RASRADGEALPALDGGVFAGIDGLALRLPYPRPVYVGFHEASRPESLPLDPVGRLIAN
                     DNPTKFTPGEDRPGPSYHVLSSRGRARPATSAVDIVLPAGSLVAAPVDGTVVEVRDYA
                     LYGRLRDWRVVIEPEGRPDLQIVLIHLEQPRVRPGDRVVAGSTPLAAVRLLPMPSHVD
                     YVLNERNPHVHLEVKPAVAPEPYDPNAPAVEPEEDDEAPV"
     gene            complement(2261..>2530)
                     /locus_tag="VNU01_13345"
     CDS             complement(2261..>2530)
                     /locus_tag="VNU01_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33649.1"
                     /translation="GRKLAELARGRQVLCVTHLAQLAAFADVHFQVSKRERDGRTVAE
                     VRPLEEAERVVELSRMLSGSPDSELAAGHAAELREAALAALPRGS"
BASE COUNT          335 a          915 c          946 g          334 t
ORIGIN      
        1 ggacccactc gatctcgacg tccacgccgt ggtggatgcc cgcgtgcgtc agcgactcca
       61 ccacggacag gtaggcgtcc ggcagcgaca cgtacttgcc caccacggcg atgcgcacgg
      121 tgtcggacgg gttgagctgg cggtgcacca tcgcggtcca gtcggcgagc tccggctgca
      181 cggccgggtc caggcccagg cggcgcacga cgaagtcgtc caggccctcc tcgcgcagca
      241 ccagcggcac cgagtacagc gactcggcgt cgtgcgccga cacgacgcct tcgatgtcca
      301 cgtcggagag cagcgcgatc ttgcgcttga gctccggcga cagcgggcgg tcggcccggc
      361 acaccacggc gtcgggctgg atcccgatgc tgcgcagctc gcgcaccgag tgctgcgccg
      421 gcttggtctt gagctctccg gtgggaccga tgaacggcac cagcgcgcag tgcacgtagc
      481 agatgttgtc ccggccgacg tcgtagcgca gctgccggat cgcctccagg aacggcaggc
      541 cctcgatgtc gccgacggtg ccgcccacct cggtgatgac gacgtcggcg tcctggccca
      601 gcgccaggat gcggcccttg atctcgtcgg tgatgtgcgg gatgacctgc acggtcttgc
      661 ccaggtagtc gccgcggcgc tccttggcga tcacggccga gtagatctgg ccggtggaga
      721 cgctggagtt gcggtgcagg ttctcgtcga tgaagcgctc gtagtggccc aggtccaggt
      781 cggtctcgcc gccgtcctcg gtgacgaaca cctcgccgtg ctcgaacggg ttcatggtcc
      841 cgggatcgac gttcacgtag gggtcgagct tctgcatcgt gacgcgcagg ccgcgcacct
      901 tgagcagcct gccgagcgag gcggccgtga tgcccttgcc gagggaggag gagacgccgc
      961 cggtgacgag aatgtgcttt gccacgctgt ccgcgccctc cttcgaccca cgggtgcgcc
     1021 agtcggcgcc atccgggggg cccgccatcc ggctgggtgc gcagcctcgg ccaccgtacc
     1081 aggggccagg gtggcgctca acgaaccggc cccggcacgc cgcgcgaccc gccctggagg
     1141 tgcggcggca ggcgtcagac cggcgcctcg tcgtcctctt ccggctccac ggcgggcgcg
     1201 ttggggtcgt agggctccgg cgccactgcc ggcttgacct ccaggtgcac gtgcgggttg
     1261 cgctcgttca gcacgtagtc cacgtggctg ggcatgggca gcagacgcac cgcggccagg
     1321 ggcgtggagc ccgccaccac gcggtcgccg gggcgcacgc gcggctgctc caggtggatc
     1381 agcacgatct gcaggtccgg ccggccctcc ggctcgatga ccacgcgcca gtcccgcagc
     1441 cgcccgtaca gcgcgtagtc gcgcacttcc accacggtgc cgtcgaccgg ggcggccacc
     1501 agcgagcccg cgggcagcac gatgtcgacg gcgctggtgg ccgggcgcgc gcggccgcgg
     1561 gaggacagca cgtgatagga ggggccggga cggtcctcac cgggggtgaa cttggtgggg
     1621 ttgtcgttgg cgatcaggcg gcccacggga tccagcggca gcgactccgg cctgctcgcc
     1681 tcgtggaagc cgacgtagac gggacgcgga tagggcaggc gcagggcgag gccgtcgatg
     1741 ccggcgaaca cgccgccgtc gagcgccggc agcgcctcgc cgtcggcacg cgaggcccgg
     1801 tcggtgcccg cgaggcggcg cgggacgtcc ccggtcgcgt ccagcggagc cggcgccgcg
     1861 gggatcgtgg cgagcgcgac ccgcgggtcc gcctggcgcg aggcggccat gccgacccag
     1921 acgacggcgg cgctgatggc cgcgagcgcc cagaccacgc cgcggggcac gcgggtgcgg
     1981 cggcgggaga cgaacgcgcc gtcacgctgc acgccgaacc gtcggcggcg ccgccggggc
     2041 gcggatgccg caccggcgtt catggcccgt cccagacggc ccagctcctt gatgtcctgc
     2101 gccaatggcc tcgctcgtgt gcgtcttcgc gttccgccgc gcgccgcaag gcgcgcggaa
     2161 aacgtccatg gcgtatgggg gaagtggggc tattcaagca catcggcggg ccgggacgcg
     2221 caaccggccg tgtccggccg gacgggtgcc cgccagcgcc tcaggagccc cgcggcaggg
     2281 ccgcgagcgc cgcctcccgc agttcggcgg cgtggccggc cgccagctcg ctgtccggcg
     2341 agcccgacag catccgcgac agctcgacca cgcgctcggc ctcctccagc ggccgcacct
     2401 cggccaccgt gcggccgtcg cgctcgcgct tggagacctg gaagtggacg tcggcgaacg
     2461 ccgcgagctg ggccaggtgc gtgacgcaca gcacctgccg cccgcgcgcg agctcggcca
     2521 gcttgcgccc
//
LOCUS       DATMKC010000462         7350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_267911, whole genome shotgun sequence.
ACCESSION   DATMKC010000462 DATMKC010000000
VERSION     DATMKC010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267911"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VNU01_13350"
     CDS             <1..207
                     /locus_tag="VNU01_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33650.1"
                     /translation="PVVAVILGVAVLREPLTWNLFAGAAVVIFGVAVADGRLRRRPAG
                     AGAEPAGVDAPVLAAEDAGRDGRH"
     gene            191..805
                     /locus_tag="VNU01_13355"
     CDS             191..805
                     /locus_tag="VNU01_13355"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HWH33651.1"
                     /translation="MTDGTDWLDLLDWRRRVAELYRRVRALRGDNMVPAHTLWIDERS
                     RLFRDHPQSPVPASERDAFGRLRSWPYDPALAFTAPLRPLPRERVDGPVSVEGHPVPL
                     LRIGAVDLPVGTLEVFWVDDYGGGLFLPFADATSGTETYGAGRYLLDSAKGADLGSAE
                     DGALHVDFNFAYQPSCAWDPAWACPLAPPANRLPAPVRAGERLK"
     gene            complement(888..1835)
                     /locus_tag="VNU01_13360"
     CDS             complement(888..1835)
                     /locus_tag="VNU01_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWH33652.1"
                     /translation="MRFMRKSAHRSRWVAMLALVLLVATGCGDSQEVAQEPEGGDDTE
                     RGTVVVGSTNFSEQEIVAEMYALVLEDAGYTVERRFQLGSREVVLPALEQGEIDLYPE
                     YVGTALEFLSGGAGEATSDTAATTEKLRERFADKGVAVLEPAPAQDRNGLVVTQETAD
                     RLGLETVSDLEAHAGELVFGGPPECPQRPLCLQGYEEAYGLEFAEFRPLDAAGPVTWA
                     ALENGDIDVALMFTTQGVIEDKGWVLLEEDAELQPAENIVPVIREEVLTDEIADLLDG
                     VSARLTTEAITALNREVDAEAADPRSVAEAWLQENGLIS"
     gene            complement(1878..2543)
                     /locus_tag="VNU01_13365"
     CDS             complement(1878..2543)
                     /locus_tag="VNU01_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33653.1"
                     /translation="MSALAQWLSDPASWWGPGGLLARTLEHAGYSLGTTLAAVAVTLP
                     VALWVGHTGRGRLAAVQLANIGRAVPTFGILLGAFVAFGYGLAPVYAALVALAIPPIM
                     VNTVVGVGQVDPEVREAAEGMGMRGWQVLLRVELPLAAPMVMAGVRTAAVQVVATATL
                     AAVVGLGGLGRPIVDGLAQGAAYSPRAQAMVVTGAIAVALLALAAELGLSAVERALTP
                     PGG"
     gene            complement(2540..3202)
                     /locus_tag="VNU01_13370"
     CDS             complement(2540..3202)
                     /locus_tag="VNU01_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWH33654.1"
                     /translation="MTAVARRFPDWDWLASHGGEVAAQLSEHVELTALAVAAGFLVAV
                     PLALLARRVPPLRGPLLGFTGVLYTVPALALFVLLLPFVGLSRANPVIGLTIYSLLIL
                     LRNTLVGLEGVPAEVRESALAMGHTRRQLLWRVEVPVALPAIVAGVRLATVSTVGLVT
                     ITALIGYGGLGQLILRGFNRTNATQLVAGLVLSVALAAVADVALAWAQRRLTPWQESR
                     TA"
     gene            complement(3199..4284)
                     /locus_tag="VNU01_13375"
     CDS             complement(3199..4284)
                     /locus_tag="VNU01_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWH33655.1"
                     /translation="MITFEAVTKRFPDGTLAVEDLDLAIPEGEIVVLVGPSGCGKTTT
                     MRLVNRMAEPTAGRVLLAGTDVRDVPAHELRRGIGYVIQQIGLFPHRSAAQNIATVPE
                     LAGWPRDRVRARVDELMALVGLPAETAGRFPHQLSGGQRQRVGVARALAADPPVLLMD
                     EPFGAVDPVVRGQLQDELLRLQQAVHKTIVFVTHDIDEALKLGDRIAVMSAPGRVRQY
                     DTPSAILARPADDFVTEFLGAERGLKRLALIPVSQVKADPGPAVPDGAPRADAVRAAE
                     AAGTDWVVVLGPEQRLLGWVWVADVAERVDGSAAREFWLRVRAEDSLRAALDAMLSTR
                     TGVAVRISGEDRYEGVLTRELLTEEIV"
     gene            complement(4376..4900)
                     /locus_tag="VNU01_13380"
     CDS             complement(4376..4900)
                     /locus_tag="VNU01_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWH33656.1"
                     /translation="MEPAALRAAVAAREPVDAREAASVSRFLRELDRLRRPFDEHADP
                     VHVTASAIVTGPRGTVLHLHKRLGLWLQPGGHIDPRDHGWEEPWEAALREAAEETGLA
                     VAHPAAGPRLVHVDVHPGPKGHTHLDVRYLLEADGDPAPGEGESRDVRWFAWDDALAV
                     ADPGLSGALAALRP"
     gene            complement(4900..6123)
                     /locus_tag="VNU01_13385"
     CDS             complement(4900..6123)
                     /locus_tag="VNU01_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HWH33657.1"
                     /translation="MNAHTEPDAAGSFEERVLAVHQGGKISIHTKVPVETREDLSLVY
                     TPGVARVCMAIHDDPALARVLTIKRNSVAVITDGSAVLGLGDIGPAAALPVMEGKAML
                     FKDFADVDAYPLCLDERDPDKLVDLIASLATGFGGINLEDISAPRCFEIEGKLRQRLD
                     IPVFHDDQHGTAVVVLAALINAAKVVGKELESLRVVVQGIGAAGVAVTNLLLAAGIPD
                     VVGVDLHGIVTPRRKEARDPIFRRLGKQTNPRGLDGPKENALRDADVFVGVSGGNSLS
                     LEHLELMAPDRIVFAMANPTPEIDPAVARDHVAVMATGRSDFPNQINNVLCFPGLFRG
                     LLDCGAREINDAMKLAAARGIAGIVDEARLSADNVIPSPFDRAVVPAVARAVVEAAEA
                     TGAARVKRSEAADAS"
     gene            complement(6206..6865)
                     /locus_tag="VNU01_13390"
     CDS             complement(6206..6865)
                     /locus_tag="VNU01_13390"
                     /inference="COORDINATES: protein motif:HMM:NF037795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWH33658.1"
                     /translation="MTPLIDATEMYLRTVWELEEEGITPIRARLVERLGLSAPAVSET
                     VHRLEEEGLLRVNDDRTLSLTDHGAQIAVSVMRKHRLAELLLTEVIGLEWEQVHNEAC
                     RWEHVISDAVEERLVELLKDPTTCPHGNPIPGAPPGDQPARVRTVAEAAKDGARVVVC
                     RISEKLQGDVDAMRFLREHGIRPGTHLDLTLERGQVIAGLDGRRVTLPPDAAELVYVA
                     A"
     gene            6971..>7350
                     /locus_tag="VNU01_13395"
     CDS             6971..>7350
                     /locus_tag="VNU01_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33659.1"
                     /translation="MPQPLTAVYLDFQSAACHRVWCWLSATSACERVEVRPFWSESPV
                     AAGGRGPWERAAPTSGLELLAMGEFARELGRRAPSDSHSSRPLDSAASSCSTAKAAST
                     KRWCATSWASTACRSWCSATGWPCA"
BASE COUNT          935 a         2727 c         2735 g          953 t
ORIGIN      
        1 cccgtggtcg ccgtgatcct gggcgtcgcg gtgctgcgcg agccgctgac gtggaacctg
       61 ttcgccggcg ccgcggtggt gatcttcggg gtggcggtcg ccgacgggcg gctgcgcagg
      121 cgccccgcgg gagccggcgc cgagccggcg ggggtggacg cgcccgtgct ggccgccgag
      181 gatgccggac gtgacggacg gcactgactg gctcgacctg ctcgactggc ggcggcgcgt
      241 cgccgagctg taccggcggg tgcgcgcgct gcgcggcgac aacatggtcc cggcgcacac
      301 gctgtggatc gacgagcgca gccgcctgtt ccgcgaccac ccgcagtcgc ccgtgccggc
      361 gagcgagcgc gacgcgttcg gcaggctgcg gtcgtggccg tacgacccgg cactggcgtt
      421 caccgcgccc ctgcgcccgc tgccgcgcga gcgggtcgac gggccggtga gcgtcgaggg
      481 ccacccggtg ccgctgctgc ggatcggcgc ggtcgacctg cccgtgggca cgctggaggt
      541 gttctgggtc gacgactacg gcggcgggct gttcctgccg ttcgcggacg cgaccagcgg
      601 cacggagacc tatggcgctg gccgctacct gctggacagc gcgaagggcg ccgacctcgg
      661 ctcggccgag gacggcgccc tccacgtgga cttcaacttc gcctaccagc catcatgcgc
      721 ctgggacccg gcctgggcct gccccctggc cccacccgcc aaccgcctgc ccgcgccagt
      781 gcgcgcgggc gagcgcctca aatagagaaa cggccgggcg gcgcgtcccg aagcggccgt
      841 aaagggatct tggtcgcgaa gggatgtgcc gcccggccgg gccctattca ggagatgagc
      901 ccgttttcct gcagccaggc ctccgccacg ctgcgcgggt cggccgcctc cgcgtccacc
      961 tcgcggttca gcgcggtgat cgcctcggtg gtgagccgcg cggacacgcc gtcgagcaga
     1021 tccgcgatct cgtcggtgag gacctcctcg cggatcaccg gcacgatgtt ctccgccggc
     1081 tggagctcgg cgtcctcctc cagcagcacc cagcccttgt cctcgatgac gccctgggtg
     1141 gtgaacatca gcgccacgtc gatgtcgccg ttctccagcg cggcccaggt cacggggccg
     1201 gcggcgtcga gcgggcggaa ctcggcgaac tccaggccgt acgcctcttc gtagccctgc
     1261 aggcacagcg gccgctgcgg gcactcgggc ggcccgccga agaccagttc gcccgcgtgc
     1321 gcctccaggt cgctgacggt ctccaggccc agccggtcgg cggtctcctg ggtgaccacc
     1381 aggccgttgc ggtcctgcgc gggtgccggc tccaggacgg cgacgccctt gtcggcgaag
     1441 cgctcccgca gcttctccgt ggtcgcggcg gtgtcactgg tggcctcgcc ggcaccgccg
     1501 ctgaggaact ccagggcggt gcccacgtac tcggggtaca ggtcgatctc gccctgctcc
     1561 agggcgggca gcacgacttc gcggctgccg agctggaagc ggcgctcgac ggtgtagccc
     1621 gcgtcctcca ggaccagggc gtacatctcc gcgacgatct cctgctcgct gaagttggtg
     1681 gagcccacca ccacggtgcc gcgctcggtg tcgtcaccgc cttccggctc ctgtgcgacc
     1741 tcttgactgt caccgcagcc ggtcgccacc agcagcacca gggccagcat ggccacccag
     1801 cgggaacggt gggcgctctt tcgcatgaat cgcatgtcgg ttctccttcg tgtcgtgtgc
     1861 ggttcggaaa ggttgtgtca gccgccgggc ggggtgagcg cgcgctcgac ggcgctcaat
     1921 cccagctcgg cggccagcgc gaggagggcc acggcgatcg cgccggtcac caccatcgcc
     1981 tgcgcgcgcg gggagtaggc ggcgccctgg gcgaggccgt cgacgatggg ccgccccagg
     2041 ccgcccaggc ccaccacggc ggccagcgtc gcggtggcca ccacctgcac ggcggcggtc
     2101 cgcacgccgg ccatgaccat cggcgcggcc agtggcagct ccacgcgcag cagcacctgc
     2161 cagccgcgca tgcccatgcc ctcggcggcc tcgcggacct ccgggtccac ctggccgacg
     2221 cccaccaccg tgttgaccat gatcggcggg atcgccaggg cgaccagcgc cgcgtacacc
     2281 ggcgccagcc cgtagccgaa ggcgacgaac gcgcccagca ggatgccgaa cgtcggcacg
     2341 gcgcggccga tgttggccag ttgcacggcg gccagccggc cgcggccggt gtggccgacc
     2401 cacagcgcca ccggcagcgt caccgcgacg gcggccagcg tggtgcccag cgagtagccg
     2461 gcgtgctcca gcgtgcgcgc gagcagcccg cctggccccc accagctcgc ggggtcggac
     2521 agccactgcg ccagggcgct cacgcggtgc ggctttcctg ccacggcgtc aggcggcgct
     2581 gtgcccacgc cagcgcgacg tcggccacgg cggccagcgc cacggacagc accaggccgg
     2641 ccacgagctg cgtggcgttg gtgcggttga agccgcgcag gatgagctgg ccgagcccgc
     2701 cgtagccgat cagagctgtg atggtgacca ggcccacggt gctcaccgtc gccaggcgca
     2761 cgccggcgac gatcgcgggc agcgccaccg gcacctccac gcgccacagc agctggcgcc
     2821 gcgtgtggcc catggccagg gccgactcgc gcacctcggc cggcacgccc tccaggccca
     2881 cgagcgtgtt gcgcagcagg atcagcagcg agtagatcgt caggccgatg acggggttgg
     2941 cgcgcgacag gcccacgaac ggcagcagca gcacgaacag cgccagggcc ggcaccgtgt
     3001 agagcacgcc ggtgaagccg agcagcgggc cgcgcagggg cggcacccgc cgcgccagca
     3061 gggcgagcgg cacggcgacg aggaagccgg ccgccacggc cagcgccgtc agctccacgt
     3121 gctcggaaag ctgcgcggcc acctccccgc cgtgggaggc cagccagtcc cagtccggga
     3181 agcgccgcgc cacggccgtc acacgatctc ctcggtgagc agctcccgcg tcagcacgcc
     3241 ctcgtagcgg tcctcgccgc tgatgcgcac cgcgacgccc gtgcgggtgg acagcatggc
     3301 gtccagcgcc gcgcgcaggg agtcctccgc ccgcacgcgc agccagaact cccgcgccgc
     3361 cgacccgtcg acgcgctcgg cgacgtcggc cacccacacc cagccgagca ggcgctgctc
     3421 cgggccgagc acgaccaccc agtccgtgcc cgccgcttcg gcggcacgca ccgcgtcggc
     3481 gcgcggcgcg ccgtcgggca ccgccgggcc ggggtccgcc ttcacctggc tcaccgggat
     3541 cagggccagg cgcttgagcc cgcgctccgc gcccaggaac tccgtgacga agtcgtcggc
     3601 cggccgggcc aggatcgccg acggcgtgtc gtactggcgc acgcggccgg gcgcggacat
     3661 cacggcgatg cggtcgccca gcttcagcgc ctcgtcgatg tcgtgcgtga cgaacacgat
     3721 ggtcttgtgc accgcctgct gcaggcgcag cagctcgtcc tggagctggc cgcgcacgac
     3781 cgggtcgacg gcgccgaacg gctcgtccat cagcagcacc ggcgggtccg cggcgagggc
     3841 gcgcgccacg ccgacgcgct ggcgctggcc gccggaaagc tggtgcggga agcgcccggc
     3901 ggtctctgcc ggcagcccga ccagcgccat gagctcgtcc acgcgcgccc gcacccggtc
     3961 gcgcggccag cccgcgagct ccgggaccgt ggcgatgttc tgcgcggcgc tgcggtgggg
     4021 gaacagcccg atctgctgga tcacgtagcc gatgccccgg cgcagctcgt gggcgggcac
     4081 gtcgcgcacg tcggtgccgg ccagcaggac gcggccggcg gtgggctcgg ccatgcggtt
     4141 gaccagccgc atggtggtgg tcttgccgca gccggaggga cccaccagca cgacgatctc
     4201 gccctcgggg atcgcgaggt ccaggtcctc gacggcgagc gtgccgtcgg ggaaccgctt
     4261 cgtcaccgcc tcgaacgtga tcacgcgggc agaacctatc gaagggccgg gggactggcg
     4321 aactcccggc ggcaacccgc gcgcgccgcg gcgcattccc gcggcgggcg ggcgctcagg
     4381 ggcgcagggc ggccagcgcg ccggacaggc cggggtcggc caccgccagc gcgtcgtccc
     4441 aggcgaacca gcgcacgtcc cggctctcgc cctcgcccgg ggcggggtcg ccgtcggcct
     4501 cgagcaggta gcgcacgtcc aggtgggtgt ggcccttggg gccggggtgc acgtcgacgt
     4561 ggaccagccg cgggcctgcg gccgggtgcg cgacggccag acccgtctcc tcggccgcct
     4621 cgcgcagcgc ggcctcccag ggctcctccc agccgtggtc gcgcgggtcg atgtgcccgc
     4681 cgggctgcag ccacagcccc aggcgcttgt gcaggtgcag gacggtgccg cgcgggccgg
     4741 tcacgatggc cgaggcggtg acgtggacgg ggtcggcgtg ctcgtcgaac ggccggcgca
     4801 ggcggtccag ctcacgcagg aagcggctga ccgacgcggc ctcgcgcgcg tccacgggct
     4861 ctcgcgcggc caccgccgcc cgcagcgccg ccggctccat caggaggcgt ccgcggcctc
     4921 ggagcgcttc acgcgggcgg cgccggtggc ctcggcggcc tcgaccaccg cgcgcgccac
     4981 cgccggcacg acggcgcggt cgaacggcga cgggatcacg ttgtcggcgg acagccgggc
     5041 ctcatcgacg atgccggcga tgccgcgcgc ggccgccagc ttcatcgcgt cgttgatctc
     5101 gcgcgcgccg cagtccagca ggccgcggaa caggcccggg aagcacagca cgttgttgat
     5161 ctggttgggg aagtcgctgc ggccggtggc catgaccgcg acatggtcgc gcgccacggc
     5221 cgggtcgatc tccggcgtcg ggttggccat cgcgaacacg atgcggtccg gcgccatgag
     5281 ctccaggtgc tccaggctga gcgagttgcc gccggagacg ccgacgaaca cgtcggcgtc
     5341 gcgcagcgcg ttctccttgg gtccgtccag cccgcgcgga ttggtctgct tgcccaggcg
     5401 gcggaagatc gggtcgcgcg cctccttgcg ccgaggcgtc acgatgccgt gcaggtccac
     5461 gcccacgacg tcggggatgc cggccgccag cagcaggttg gtcacggcca cgcccgccgc
     5521 gccgatgccc tgcaccacca cgcgcagcga ctccagttcc ttgcccacca ccttggccgc
     5581 gttgatcagc gcggccagga ccaccacggc cgtgccgtgc tggtcgtcgt ggaacacggg
     5641 gatgtccagg cgctggcgca gcttgccctc gatctcgaag cagcgcggcg cggagatgtc
     5701 ctccaggttg atgccgccga agccggtggc cagcgacgcg atcaggtcga ccagcttgtc
     5761 ggggtcgcgc tcgtccaggc acagcgggta ggcgtcgacg tcggcgaagt ccttgaacag
     5821 catcgccttg ccctccatga cgggcagggc tgccgcgggg ccgatgtcgc ccaggcccag
     5881 cacggcggag ccgtccgtga tcacggcgac gctgttgcgc ttgatggtca ggacgcgcgc
     5941 cagggccggg tcgtcgtgga tggccatgca gacgcgcgcg acgccggggg tgtagaccag
     6001 cgacaggtcc tcgcgcgtct cgacggggac cttggtgtgg atggagatct tgccgccctg
     6061 atggaccgcc agcacgcgct cctcgaagga gccggcggcg tcgggttcgg tgtgcgcgtt
     6121 catcgtggtc gccgaggata gccgcgccgc ctggcggccc gggacacccc ggccggcgcg
     6181 atggacaggt ctggggccgg tcagctcacg cggcgacgta gacgagctcg gccgcgtccg
     6241 gcgggagcgt gacccggcgc ccgtcgagcc cggcgatcac ctggccgcgc tccagggtca
     6301 ggtcgaggtg cgtgcccggg cggatgccgt gctcgcgcag gaagcgcatc gcgtcgacgt
     6361 cgccctggag cttctcggag atccggcaca cgaccacccg cgcgccgtcc ttggcggcct
     6421 ccgcgacggt gcgcacgcgg gcgggctggt cgccgggcgg cgcgcccggg atcgggttgc
     6481 cgtgcgggca ggtggtcggg tccttgagca gctcgaccag gcgctcttcc accgcgtcgg
     6541 agatgacgtg ctcccagcgg caggcctcgt tgtggacctg ctcccactcc aggccgatga
     6601 cctcggtgag cagcagctcc gccaggcggt gcttgcgcat gacgctgacc gcgatctggg
     6661 cgccgtggtc ggtgagcgac agcgtgcggt cgtcgttgac gcgcagcagg ccctcttcct
     6721 ccagacggtg cacggtctcg ctgacggccg gcgcggacag gcccaggcgc tccaccaggc
     6781 gggcgcggat cggggtgatg ccttcttcct ccagctccca cacggtgcgg aggtacatct
     6841 cggtggcatc gatcagcggc gtcatggccc aatgctatcc ccctgccccg cgggggcacg
     6901 cgggggcgta gcctgcaggc ggcggaggta tgggggtgcc aggcttgtgc cgcgacagga
     6961 gacgcgaccg atgccccaac cgctgaccgc tgtgtacctc gacttccagt ccgccgcctg
     7021 ccaccgggtg tggtgctggc tgtcggccac gtccgcgtgc gagcgcgtgg aggtgcgtcc
     7081 gttctggagc gagagccccg tcgcggccgg gggccgcggc ccgtgggagc gcgccgcgcc
     7141 cacctccggg ctggagctgc tggccatggg cgagttcgcc cgcgagctgg ggcgccgcgc
     7201 gcccagcgac agccacagct ccaggccgct tgactcggcc gcgtcctcgt gcagcacggc
     7261 gaaggccgcc tccacgaagc ggtggtgcgc gacgagctgg gcgtcgacgg cgtgccggtc
     7321 ctggtgttcg gcgaccggct ggccgtgcgc
//
LOCUS       DATMKC010000463         2823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_216198, whole genome shotgun sequence.
ACCESSION   DATMKC010000463 DATMKC010000000
VERSION     DATMKC010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216198"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VNU01_13400"
     CDS             complement(<1..1298)
                     /locus_tag="VNU01_13400"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWH33660.1"
                     /translation="MSAFTFTGARVVDPATGLDGVANVTVDGETITAVGDQVEGTVHD
                     ATGLVLAPGLVDLHVHLRQPGREDAETVESGTAAAAAGGFTAVCPMANTDPVADHAGV
                     VELVLRLAREAGHCDVYPVGAITRGLRGEELAEMGSMAAVGVRCFSDDGMPVASARVM
                     RRALEYARTWDAIVCNHSEEPTLTDGAQLNEGELSSLLGLTGWPREAEEVMVARDLIL
                     AGGLGARLHVPHVSTAGTVGLVREAKRRGVRVTAEAAPHHFTLVDEAAKGYDPVFKVN
                     PPLRTKDDVEAIRAGLADGTIDAIATDHAPHSHEHKEQEWAHAPCGMLGLEVSLAVTL
                     TELVDLDAPAGERGDGVLTLAEALACLSTKPARSRDIHGHGGPVEAGAAANLVLFDPS
                     ARWTAVPVDVPAARGLGGQAGERLGQGQHAVAALTGGRVEV"
     gene            complement(1295..2236)
                     /locus_tag="VNU01_13405"
     CDS             complement(1295..2236)
                     /locus_tag="VNU01_13405"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWH33661.1"
                     /translation="MIEHLLSMEDLSAEDVNAVLDAAESFAELGDRTVKKVPTLRGRT
                     VCNLFYEDSTRTRLSFELAAKRLSADVLNFSAKGSSVSKGESLKDTALTLQAMGVDAI
                     VLRHSASGAPLALTQWVDAKVLNAGDGAHQHPTQALLDLFTIRRHLGRLEGLRVAVVG
                     DVLHSRVARSLVQGLRTMGAEVTLVAPPTLLPPRPESWGTAVTSDLDGVLGKTDVLYL
                     LRVQHERMQEQFFPSPREYARLWGVDRDRLARLPDGAIVMHPGPMNRGMEVSADVADA
                     PNAVITDQVRNGIAVRMSCLYLMLGGHGPDGAGKEDA"
     gene            complement(2233..2751)
                     /gene="pyrR"
                     /locus_tag="VNU01_13410"
     CDS             complement(2233..2751)
                     /gene="pyrR"
                     /locus_tag="VNU01_13410"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWH33662.1"
                     /translation="MSAEILSADDVARAVRRIAHEMIERGELGQTVLLGIRTRGVPLA
                     ERIAEAIADIDGARPPVGALDVTLYRDDYASAGPRPLAETHVPEDLDDRVVVLVDDVL
                     YTGRTIRAAMDAVMDLGRPRAVRLAVLVDRGHRELPIRADYVGKNLPTAPEQSVKVRL
                     GETDGADEVVVA"
BASE COUNT          355 a         1045 c         1031 g          392 t
ORIGIN      
        1 acctcgacgc gcccgccggt gagcgcggcg acggcgtgct gaccttggcc gaggcgctcg
       61 cctgcctgtc caccaagccc gcgcgcagcc gggacatcca cgggcacggc ggtccagcgc
      121 gcggacgggt cgaacagcac caggttcgcc gccgctcccg cctcgacggg accgccgtgc
      181 ccgtggatgt cccggctgcg cgcgggcttg gtggacaggc aggcgagcgc ctcggccaag
      241 gtcagcacgc cgtcgccgcg ctcaccggcg ggcgcgtcga ggtccaccag ctcggtcagc
      301 gtcacggcca gcgacacctc caggccgagc atgccgcagg gcgcgtgcgc ccactcctgc
      361 tccttgtgct cgtgggagtg gggggcgtgg tcggtggcga tcgcgtcgat ggtgccgtcg
      421 gccaggccgg cgcgaatggc ctccacgtcg tccttggtgc gcagcggcgg gttgaccttg
      481 aacaccgggt cgtagccctt ggcggcctcg tcgacgagcg tgaagtgatg cggcgcggcc
      541 tcggccgtga cgcgcacgcc ccggcgcttg gcctctcgca ccagccccac ggtgcccgcc
      601 gtggacacgt gcgggacgtg caggcgggcg cccagcccgc ccgcgaggat caggtcgcgg
      661 gcgaccatca cctcctcggc ctcgcgcggc cagccggtca gccccagcag gctggacagc
      721 tcgccctcgt tgagctgcgc gccgtcggtc agggtgggct cctccgagtg gttgcacacg
      781 atcgcgtccc acgtgcgcgc gtactccagc gcgcggcgca tcacgcgggc gctggccacc
      841 ggcatgccgt cgtcgctgaa gcagcgcaca ccgacggccg ccatggagcc catctcggcc
      901 agctcctcgc ctctgaggcc gcgcgtgatg gctccgaccg ggtagacgtc gcagtggccg
      961 gcctcgcgcg ccaggcgcag caccagctcc accacgccgg cgtggtcggc cacggggtcc
     1021 gtgttcgcca tggggcacac ggcggtgaag ccccccgccg cggccgcggc cgtaccggac
     1081 tcgacggtct cggcgtcctc acggcccggc tggcgcaggt gcacgtgcag gtcgacgagc
     1141 ccgggcgcga gcaccaggcc cgtggcgtcg tgcaccgtgc cctcgacctg gtcgcccacc
     1201 gcggtgatgg tctcgccgtc gacggtgacg ttggccaccc cgtccaggcc cgtcgcgggg
     1261 tcgaccacgc gggcgccggt gaacgtgaac gcgctcacgc gtcctccttg ccggcgccgt
     1321 ccgggccgtg gccgccgagc atcaggtaga ggcagctcat gcgcaccgcg atgccgttgc
     1381 gcacctggtc ggtgatgacc gcgttggggg cgtcggccac gtcggcgctg acctccatgc
     1441 cgcggttcat gggaccgggg tgcatgacga tggcgccgtc cgggaggcgc gcgagccggt
     1501 cgcggtccac accccacagg cgcgcgtact ccctcggcga cgggaagaac tgctcttgca
     1561 tgcgctcgtg ctggacgcgc agcaggtaca gcacgtcggt cttgcccagg acgccgtcga
     1621 ggtcggacgt cacggcggtg ccccagctct ccggccgggg cggcagcagc gtcggcgggg
     1681 cgaccagcgt cacctcggcg cccatggtgc gcagcccctg caccaggctg cgcgccaccc
     1741 ggctgtgcag cacgtcgccg accacggcca cgcgcagtcc ctccaggcgg cccaggtggc
     1801 ggcggatggt gaacaggtcc agcagcgcct gcgtggggtg ctggtgggcg ccatcaccgg
     1861 cgttgagcac cttggcgtcg acccactgcg tcagcgccag cggcgcgccg gaggcggagt
     1921 ggcgcaggac gatcgcgtcc acgcccatcg cctgcagcgt cagggcggtg tccttcagcg
     1981 actcgccttt gctgacgctg gagcccttgg cgctgaagtt cagcacgtcg gcggacaggc
     2041 gcttggcggc cagctcgaag ctcaggcgcg tacgggtgga gtcctcgtag aacaggttgc
     2101 acacggtgcg cccgcgcagc gtcggcacct tcttgacggt gcggtccccg agttcggcga
     2161 acgactccgc ggcgtcgagc accgcgttga cgtcctcggc ggacaggtcc tccatcgaca
     2221 gcaggtgctc gatcacgcca ccaccacctc gtcggcgccg tcggtctcac cgaggcgcac
     2281 tttgacggac tgttccggcg ccgtgggcag gttcttgccg acgtagtcgg cgcggatcgg
     2341 cagctcccgg tgcccgcggt cgaccagtac cgcgagacgc acggctcgcg gccggcccag
     2401 gtccatgacg gcgtccatcg cggcgcggat ggtgcgcccg gtgtagagca cgtcgtcgac
     2461 cagcacgacc acgcggtcgt ccaggtcctc ggggacgtgc gtctcggcga gcggccgggg
     2521 cccggcgctg gcgtagtcgt cccggtacag cgtgacgtcg agggcgccca cgggcggccg
     2581 cgcgccgtcg atgtcggcga tcgcctcggc gatgcgctcg gccagcggca ccccgcgcgt
     2641 ccggatgccg agcaggacgg tctgccccag ctcaccgcgc tcgatcatct cgtgggcgat
     2701 gcggcgaacg gcccgcgcca cgtcgtcggc ggacaggatc tcggcgctca tgaaaaaacg
     2761 tcctctcccg cggcggggaa ggacgtccgg gcatgcctgt gcaaagtggg cccctttccg
     2821 gcc
//
LOCUS       DATMKC010000464         8322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_39057, whole genome shotgun sequence.
ACCESSION   DATMKC010000464 DATMKC010000000
VERSION     DATMKC010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39057"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1594
                     /locus_tag="VNU01_13415"
     CDS             203..1594
                     /locus_tag="VNU01_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33663.1"
                     /translation="MLADLVARCLAGGRDALVLSPDPASPVPAAALAQAPGAVADLRG
                     TDDPPSRYRAFLRGRLGHARVAVGERGAVLAPLGALGLVVVEDEANPAWKEQRSPRHH
                     AREVALARAHLAGAVCVLLSDLPSAQLWRHLQDGHVAEVAVDRALERRRAPRVEVVDR
                     SDPRPGARRTRLSDASSRALAAAVRDGGAGVVLAVRGGEGTALACRRCGRRRQCPTCA
                     GSLKAAGEGWQCAACGWAGPAFACVSCGAEETAPLAAGAGRLAQELAKAHPDAEVTRM
                     EGFDAPGPARLPAIAVMTRGSVVRRPAWLGGRQADVVVLPDADALLNRPALDATEDAL
                     RLWFAVGRWTGHVVVQTDEPNHPAVQALVRWDPAGFWSREAERRAPLAFPPAGSLVRL
                     RAPADAAADVAGALRDALPPGDQVLGPDPEGGLLVKSAHLRGTLDALAPLRHAWAKAN
                     RKVRVDVDPVSAG"
     gene            1633..2187
                     /gene="def"
                     /locus_tag="VNU01_13420"
     CDS             1633..2187
                     /gene="def"
                     /locus_tag="VNU01_13420"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWH33664.1"
                     /translation="MALLPIRIFGDPVLRSRAREVTEFDDALRRLADDMLETMRAAQG
                     VGLAANQVGVLKRLFTFEAHLDDAEEPLTGALVNPEIIETSEEVQEGDEGCLSFPGLF
                     YPASRPLRARLRARDVHGEPLELVGEGLLARILLHELDHLDGILFIDHLARHDRKEAM
                     RRIRDGELERNLYTTDDEPEAEAL"
     gene            2184..3119
                     /gene="fmt"
                     /locus_tag="VNU01_13425"
     CDS             2184..3119
                     /gene="fmt"
                     /locus_tag="VNU01_13425"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWH33665.1"
                     /translation="MRIVFFGTPEAAVPSLEAFVADPDTEVVAVVTNPDRPSGRGYKL
                     TPPPVKAAAERHGLPVWQPEKPREILDHLLALRLDACAVVAYGALLPQDVLDAGGKGF
                     VNLHFSVLPAWRGAAPVQHALLAGDSETGVTCFVLDKGMDTGDVLLVERTPVAPDETA
                     GELMARLADLGAPVLVRAVHGLVDGGIVPVPQDHGAATLAPKIGNDDARLDWDLPAAR
                     LHNQVRGLNPVPGAFTTFGGDRLKVHRARVADAAGAPGNAPGEVVAVDEDGPVVACGD
                     GALLLTEVQPAGKPRMSGQAFANGYRPLGAVLGRE"
     gene            3116..4543
                     /locus_tag="VNU01_13430"
     CDS             3116..4543
                     /locus_tag="VNU01_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWH33666.1"
                     /translation="MTPQPRDAKPAPGSAPGAGAEAPKEGLATRRAAYRALRRIRERQ
                     AYSPPVVQRALDTLPPRERGLAANLVYETLRWQGTLDWALGQVLTRPIGEVEDDLRDI
                     LRMGAWQLLYGRMPDRAVVDTSVALARGEVGERVAGFTNGVLRNLARRKDALPWPPTD
                     DPRGLALATGSPVWVAERAIARFGARAEAVLRASAVAPGLTLRAVARDGEDPGAVRDA
                     LVAELRAAGVDASAGRWAPEAVRAPGADPMALDAVRQGRATPQDESSMLVTRALARAL
                     EEAGGGDRVADLCAGPGGKATHLAQLGLRVTATDVQDARAAMVAEQAVRLGLSERVDA
                     LVRDARSPDLSAATFDAVLVDAPCSGLGVTRRRPEVAWRREPEDPARLSGLQLEILGA
                     AAALVRPGGVLLYSVCTWPAEETAGTAAGFLAVEGDRFTALDPATLLPEGAGTRLPGD
                     PGVQLDPDHDGTDGMYVCGFRRLRG"
     gene            4571..5506
                     /locus_tag="VNU01_13435"
     CDS             4571..5506
                     /locus_tag="VNU01_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWH33667.1"
                     /translation="MRDTILVVDNEPDILRFVDVNLRLEGFDVVTAADGEEALERAFE
                     LRPALIVLDVMMPKLDGFEVCRRLRADSRTRMIPVIILTAKTTMIDKVVGLTAGADDY
                     VLKPFDPIELVARVRTTLKRSAELLDSSPLTGLPGNHAIGQELARRLADDAAIAAIYA
                     DLNEFKAFNDRYGFLKGDEVIVLLADCLREALREHAGDDGFLGHIGGDDFIALCTPAQ
                     VPLVCERVIELFDGRIPGLYDPEESARGWIEIEDRRGELRRYRIMTVAMGVATTERRQ
                     FSDHRELVQVATEMKNYVKRNRHSSAYAVDVRADD"
     gene            5544..6773
                     /locus_tag="VNU01_13440"
     CDS             5544..6773
                     /locus_tag="VNU01_13440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWH33668.1"
                     /translation="MSTTALPASARAWIAVVTATGAAATALVTLRWGVAAGMQLSAVL
                     LCLGVTALEWPFRALLRAVGEPDPWAFLGPMVVWLVGMPWAPALAVVAVLGSCALDRV
                     PLRKRVFNSGLRATQAVFAGVVLSGVAGMVPPLPAGVVTQPRGVVALLVAQVAWWASG
                     WVLVSVMVALASATSLARELRTGVATDFLSSFLFAVVGPMALLALAYQPLILPLLALP
                     LGGAIATGQRAWRRRQEARTDALTGLPNRLSIYEETRAALYHAGRRGLSAAICLIDLD
                     GFKPVNDTHGHETGDAVLRALSRRLEHACADERSFVGRLGGDEFLVLWRDHASEGDYR
                     RRAEAVRALLREPMRVERTPVALDASLGVAVFPSDGGALDALLRRADRAMYRAKAGGG
                     GVVVAGEPAEGSSRAGR"
     regulatory      6818..6977
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7100..8200
                     /gene="ribD"
                     /locus_tag="VNU01_13445"
     CDS             7100..8200
                     /gene="ribD"
                     /locus_tag="VNU01_13445"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWH33669.1"
                     /translation="MHDPVDVGWMRRALELAERGRHTVSPNPLVGCVLVAGGDVVGEG
                     WHQRAGQAHAEVLALAEAGERARGAVAYVTLEPCAHTGRTGPCADALVAAGVSRVVAA
                     LADPHPLAAGGARRLRAAGVDVALGVCEDEARAQNEVFLHGVATGRPFVTLKCAVSVD
                     GRIAAADGTSQWLTGPAARSLAHELRARADAVVVGSGTVLADDPALTVRLPGYAGPQP
                     LRVVLDGRARTSASHRVLDGEAPVLVGVRPEAEGGAADKLRDAGAQVVALPRDADGGL
                     GALLDELWRREVRSVLVEGGARVLGAFLGAGLWDRLVVHVAALLLGEAGRPALVGDGI
                     ATLAGAPRLRLERVEQAGDDVVCTYRPGRQEG"
BASE COUNT          925 a         3065 c         3320 g         1012 t
ORIGIN      
        1 ccagcgcggc ggcgggaacc ggtgacgcgg ggtcgggcga cagcaccagg gcgtcgcgcc
       61 cgccggccag gcagcgcgcc accaggtcgg cgaccagggc ggcgctgtcg tcgccgggca
      121 gcggccgcag ccaccacgcg ccacctcccg ccgtcgcgcc gctcacggcg ttgaggagcg
      181 ccgaggcgcg atacggccgc cagtgctcgc cgacctggtg gcgcgctgcc tggccggcgg
      241 gcgcgacgcc ctggtgctgt cgcccgaccc cgcgtcaccg gttcccgccg ccgcgctggc
      301 gcaggcgccg ggcgccgtcg cggacctgcg cggcacggac gacccgccgt cgcgctaccg
      361 cgcgttcctg cgcggcaggc tgggccacgc gcgcgtcgcg gtgggggagc gcggcgcggt
      421 cctggcgccg ctgggcgcgc tcggcctggt ggtcgtggag gacgaggcga acccggcgtg
      481 gaaggagcag cgcagcccgc gccaccacgc ccgcgaggtc gcgctggcgc gcgcccacct
      541 ggccggcgcc gtgtgcgtgc tgctgtcgga cctgccgagc gcgcagctgt ggcgccacct
      601 gcaggacggc cacgtggccg aggtggcggt ggaccgcgcg ctggagcggc gccgggcgcc
      661 gcgcgtggag gtggtcgacc gctccgaccc gcgccccggc gcccggcgca cccgcctgtc
      721 ggacgcgtcg tcccgggcgc tggccgctgc cgtgcgcgat ggcggcgccg gcgtcgtgct
      781 ggcggtgcgg ggcggcgagg gcaccgcgct ggcctgccgc cgctgcggca ggcggcggca
      841 gtgcccgacg tgcgccggct cgctcaaggc cgcgggcgaa gggtggcagt gcgcggcgtg
      901 cgggtgggcc gggcctgctt tcgcctgcgt gtcctgcggc gccgaggaga ccgcgccgct
      961 cgccgcgggc gccggccgcc tggcccagga gctcgccaag gcgcacccgg acgccgaggt
     1021 cacccgcatg gagggcttcg acgcacccgg tccggcgcgc ctgcccgcga tcgcggtgat
     1081 gacgcgcggc agcgtggtgc gccgcccggc gtggctcggc ggccgccagg ccgacgtcgt
     1141 ggtgctgccc gacgcggacg cgctgctgaa ccgcccggcc ctggacgcca cggaggacgc
     1201 cctgcgcctg tggttcgcgg tcggccgctg gaccggtcac gtcgtggtgc agaccgacga
     1261 gccgaaccac ccggcggtgc aggcgctggt gcgctgggac cccgcaggct tctggtcgcg
     1321 cgaggcagag cggcgcgcgc cgctggcgtt cccgccggcg ggctcgctgg tgcggctgcg
     1381 cgcccccgcg gacgcggccg cggacgtcgc cggggcgctg cgcgacgccc tgccgccggg
     1441 cgaccaggtg ctggggccgg acccggaagg gggcctgctt gtgaagtcgg cgcatctgcg
     1501 tggcacgctt gacgccctcg cgccgctgcg ccacgcctgg gcgaaggcga accgcaaggt
     1561 gcgcgtggac gtcgacccgg tctcggccgg gtagagcccc gagcacatcc ccgagaggag
     1621 cgacccgacc gcatggccct gcttcccatc cggatcttcg gcgacccggt gctgcgcagc
     1681 cgcgcgcgcg aggtcaccga gttcgacgac gcgctgcggc ggctcgccga cgacatgctc
     1741 gagacgatgc gcgcggcgca gggcgtcggg ctggccgcca accaggtcgg cgtgctcaag
     1801 cggctgttca ccttcgaggc gcacctggac gacgcggagg agccgctgac cggcgcgctg
     1861 gtcaacccgg agatcatcga gacctccgag gaggtgcagg agggcgacga gggctgcctg
     1921 tccttcccgg ggctgttcta cccggcgtcc cgcccgctgc gggcccggct gcgcgcccgg
     1981 gacgtccacg gggagccgct ggagctggtg ggcgagggcc tgctcgcgcg gatcctcctg
     2041 cacgagctcg accacctcga cgggatcctg ttcatcgacc acctggcgcg ccacgaccgc
     2101 aaggaggcca tgcgccgcat ccgcgacggc gagctggaac gcaacctgta caccactgac
     2161 gacgagccgg aggcggaggc gctgtgagga tcgtgttctt cggcaccccc gaggcggccg
     2221 tcccgtcgct ggaggcgttc gtggccgacc cggacaccga ggtcgtggcc gtggtcacca
     2281 acccggaccg gcccagcggc cgcggctaca agctcacgcc cccgccggtg aaggccgccg
     2341 ccgagcgcca cggcctgccc gtgtggcagc cggagaagcc gcgcgagatc ctggaccacc
     2401 tgctggcgct gcgcctggac gcctgcgccg tggtcgccta cggcgcgctg ctgccccagg
     2461 acgtgctgga cgccggcggc aagggcttcg tgaacctgca cttctcggtc ctgccggcct
     2521 ggcgcggcgc cgcgccggtg cagcacgcgc tgctggcggg ggacagcgag accggcgtga
     2581 cgtgcttcgt gctggacaag ggcatggaca ccggcgacgt gctgctggtg gagcgcaccc
     2641 cggtggcccc cgacgagacc gccggcgagc tgatggcgcg ccttgcggac ctgggcgccc
     2701 ccgtgctggt gcgcgccgtc cacggcctgg tcgacggcgg catcgtgccc gtgccgcagg
     2761 accacggcgc ggccacgctg gcccccaaga tcggcaacga cgacgcgcgc ctggactggg
     2821 acctgcccgc ggcgcgcctg cacaaccagg tgcgcgggct caaccccgtg cccggcgcgt
     2881 tcaccacgtt cggcggcgac cgcctcaagg tgcaccgcgc ccgcgtggcg gacgcggcgg
     2941 gcgcgcccgg gaacgctccc ggggaggtgg tggcggtcga cgaggacggt cccgtggtgg
     3001 cctgcggcga cggcgccctg ctgctcaccg aggtgcagcc cgccggcaag ccgcgcatga
     3061 gcggccaggc cttcgccaac ggctaccgcc cgctgggcgc cgtgctgggc cgggagtgac
     3121 cccgcagccg cgcgacgcca agcccgctcc tgggagcgcc cccggggccg gggccgaggc
     3181 gcccaaggag gggctggcga cgcgccgggc ggcctaccgc gcgctgcggc gcatccgcga
     3241 gcgccaggcg tactcgccgc cggtggtgca gcgcgcgctg gacaccctgc cgccgcgcga
     3301 gcgcggcctg gcggcgaacc tcgtctacga gaccctgcgc tggcagggca cgctggactg
     3361 ggcgctgggc caggtgctga ccaggcccat cggcgaggtc gaggacgacc tgcgcgacat
     3421 cctgcgcatg ggcgcctggc agctcctgta cgggcgcatg ccggaccgcg ccgtggtgga
     3481 caccagcgtc gcgctggccc gcggcgaggt cggcgagcgc gtcgcgggct tcaccaacgg
     3541 cgtgttgcgc aacctcgccc ggcgcaagga cgcgctgccg tggccgccca cggacgaccc
     3601 gcgcggcctg gcgctggcga ccgggtcgcc ggtgtgggtc gccgagcggg ccatcgcgcg
     3661 gttcggcgcc cgcgcggagg cggtgctgcg ggcgagcgcc gtggccccgg gcctgacgct
     3721 gcgcgcggtc gcgcgcgacg gcgaggaccc gggcgcggtc cgcgacgcgc tggtggcgga
     3781 gctgcgcgcg gccggcgtcg acgcctcggc gggacgctgg gcgcccgagg cggtgcgcgc
     3841 accgggcgcg gacccgatgg cgctggacgc ggtgcgccag ggccgcgcca ccccgcagga
     3901 cgagtcctcc atgctggtca cgcgcgcgct cgcgcgggcg ctggaagagg ccggcggcgg
     3961 tgaccgcgtc gcggacctgt gcgccgggcc cggcggcaag gccacgcatc tggcgcagct
     4021 cgggctgcgc gtcaccgcca cggacgtcca ggacgcgcgc gccgcgatgg tcgccgagca
     4081 ggccgtccgc ctggggctgt cggagcgggt ggacgcgctg gtgcgcgacg cccgcagtcc
     4141 ggacctgtcc gcggccacgt tcgacgcggt gctcgtggac gcgccgtgca gcggcctggg
     4201 ggtcacgcgg cggcgccccg aggtcgcctg gcggcgcgag ccagaggacc ccgcgcggct
     4261 gtcgggcctg cagctggaga tcctgggcgc cgccgcggca ctggtgcgcc ctggcggggt
     4321 gctgctgtac agcgtgtgca cctggccggc tgaggagacc gccggcacgg ccgcgggctt
     4381 cctggccgtg gaaggcgacc gcttcacggc gctggacccc gcgacgctgc tgccggaggg
     4441 cgccggcacg cgccttcccg gcgaccccgg cgtgcagctg gacccggacc acgacggcac
     4501 cgacgggatg tacgtgtgcg gattccgccg cctgcggggc tgagggcccc gctttcgtta
     4561 cccttgggcg atgcgggaca ccatcctggt cgtcgacaac gagccggaca tcttgcggtt
     4621 cgtcgacgtc aacttgcgcc tcgagggctt cgacgtcgtc acggcggccg acggcgagga
     4681 ggccctggag agggcgttcg agctgcggcc ggcgctgatc gtgctggacg tgatgatgcc
     4741 gaagctggac ggcttcgagg tctgccgccg cctgcgcgcg gactcccgca cgcgcatgat
     4801 cccggtcatc atcctcaccg ccaagaccac gatgatcgac aaggtcgtgg gcctgaccgc
     4861 cggcgccgac gactacgtgc tcaagccgtt cgacccgatc gagctggtcg cgcgcgtgcg
     4921 caccacgctg aagcgctccg cggagctgct ggacagctcg ccgctgaccg gcctgccggg
     4981 caaccacgcg atcgggcagg agctcgcccg ccgcctggcg gacgacgccg cgatcgccgc
     5041 catctacgcc gacctcaacg agttcaaggc cttcaacgac cgctacggct tcctcaaggg
     5101 cgacgaggtc atcgtcctgc tggccgactg cctgcgggag gcgctgcgcg agcacgccgg
     5161 cgacgacggc ttcctgggcc acatcggcgg cgacgacttc atcgccctgt gcacgccggc
     5221 gcaggtgccg ctggtgtgcg agcgcgtgat cgagctgttc gacgggcgca tccccgggct
     5281 gtacgacccg gaggagtcgg cgcgggggtg gatcgagatc gaggaccggc gcggggagct
     5341 gcggcgctac cgcatcatga cggtggccat gggggtggcc accaccgagc gccgccagtt
     5401 ctccgaccac cgggagttgg tgcaggtcgc gacggagatg aagaactacg tcaagcgcaa
     5461 ccgccacagc tccgcctacg ccgtggacgt gcgcgcagac gactagacga ccgaccgggc
     5521 gactggggga gcgcgtgccc gacttgtcca cgacggctct gccggccagc gcgagggcgt
     5581 ggatcgccgt cgtcaccgcg accggtgccg cggcgacggc cctggtgacg ctgcgctggg
     5641 gcgtggccgc cggcatgcag ctgtccgcgg tgctgctgtg cctcggcgtg accgcgctcg
     5701 agtggccgtt ccgcgcgctg ctgcgcgccg tgggcgagcc cgacccctgg gcgttcctgg
     5761 ggccgatggt ggtctggctc gtcggcatgc cgtgggcccc cgcgctggcc gtcgtcgcgg
     5821 tactgggcag ctgcgcgctc gacagggtcc cgctgcgcaa gcgcgtgttc aactccgggc
     5881 tgcgcgcgac gcaggccgtg ttcgccggtg tggtcctgtc gggggtcgcc ggcatggtgc
     5941 cgcccctgcc ggcgggcgtg gtgacccagc cccggggcgt cgtcgccctg ctggtggcgc
     6001 aggtggcgtg gtgggccagc ggctgggtgc tggtgagcgt catggtggcg ctggcgtcgg
     6061 ccacgtcgct ggcccgggag ctgcgcacgg gtgtcgccac ggacttcctg tccagcttcc
     6121 tgttcgccgt cgtggggccc atggcgctgc tcgcgctggc ctaccagccg ctgatcctgc
     6181 cgctgctggc gctgccgctc ggcggcgcga tcgccacggg gcagcgcgcg tggcggcgcc
     6241 gccaggaggc gcgcaccgac gccctgaccg ggctgcccaa ccgcctgtcg atctacgagg
     6301 agacgcgggc cgcgctctac cacgcgggcc gccgcggcct gtcggcggcg atctgcctga
     6361 tcgacctgga cgggttcaag cccgtcaacg acacccacgg gcacgagacc ggcgacgcgg
     6421 tgctgcgcgc gctgagccgc cggctggagc acgcctgcgc cgacgagcgc tcgttcgtgg
     6481 gacgcctggg cggcgacgag ttcctggtcc tgtggcgcga ccacgcgtcc gaaggggact
     6541 accggcgccg cgccgaggcg gtgcgcgccc tgctgcgcga gccgatgcgc gtggaacgca
     6601 cgccggtggc gctggacgcc tcgctcggcg tggcggtgtt cccctccgac gggggcgcgc
     6661 tcgacgccct gctgcggcgc gcggaccgcg cgatgtaccg cgccaaggcg ggcggcgggg
     6721 gcgtggtcgt ggccggtgag cccgccgagg ggtcctcgcg cgcgggccgc tgagttgcgc
     6781 cgcgcgggcc ccgaagttac cgttgagtaa ctaagagaag cgccttcggg gcggggtgga
     6841 actccccacc ggcggtagcc cggccgaccc ggccggggag cccgcgaccc ggacgcagcg
     6901 tcgaggcgac gcggcgaaag gtggatctgg tccaagtcca gagccgacgg tcacagtccg
     6961 gatgagagaa ggtgcgtgac gcgagcgcgg cgtcggctct gccatcccgg gacatccggc
     7021 ggcggcaggg acgatcggcc gtccgctccg cgcacccctt gccccgtggc acggcagccc
     7081 ggcgaggagg tggacgcgaa tgcacgaccc ggtcgacgtc ggctggatgc gccgcgccct
     7141 ggagctcgcc gagcgcggcc gccacaccgt ctcccccaac cccctggtgg gctgcgtgct
     7201 cgtggccggc ggcgacgtcg tcggcgaggg ctggcaccag cgcgccggcc aggcccacgc
     7261 cgaggtgctc gccctggcgg aggcgggcga gcgcgcgcgg ggcgcggtcg cgtacgtgac
     7321 cttggagccc tgcgcgcaca ccggccggac ggggccgtgc gcggacgcgc tcgtggccgc
     7381 gggcgtctcc cgcgtggtgg ctgcgctggc cgatccccac ccgctcgccg caggcggtgc
     7441 ccggcggctg cgcgccgccg gcgtcgacgt ggcgctgggc gtgtgcgagg acgaggcgcg
     7501 ggcgcagaac gaggtgttcc tgcacggcgt ggccaccggg cgcccgttcg tcacgctcaa
     7561 gtgcgcggtc agcgtggacg ggcgcatcgc cgccgcggac ggcacgtcgc aatggctgac
     7621 cgggccggcg gcacgcagcc tcgcgcacga gctgcgcgcg cgggccgacg ccgtcgtcgt
     7681 gggcagcggc accgtgctgg ccgacgaccc ggcgctgacg gtgcgactgc cgggctacgc
     7741 cggaccgcag ccgctgcgcg tcgtgctgga cggccgcgcc cgcacgtcgg cctcccatcg
     7801 tgtcctggac ggcgaggccc cggtgctggt cggggtccgt cccgaggccg agggcggcgc
     7861 ggccgacaag ctgcgcgacg ccggcgccca ggtcgtggcc ctgccgcgcg acgccgacgg
     7921 cggcctgggc gcgctgctgg acgagctgtg gcggcgggag gtgcgctccg tcctggtgga
     7981 gggcggcgcg cgggtgctcg gcgccttcct gggcgcgggg ctgtgggacc gcctggtcgt
     8041 gcacgtcgcg gccctgctgc tcggcgaagc cgggcgtccg gcgctggtgg gcgacggcat
     8101 cgccacgctg gccggcgcgc cgcggctgcg cctggagcgc gtcgagcagg ccggcgacga
     8161 cgtcgtctgc acgtaccgcc ccggccggca ggagggctga gccatgttca ccggaatcgt
     8221 cgaggagttg ggcgcgatcg cgcacgtcgc gcggcacggc gcgggcgccg agctggcggt
     8281 gcgctgcgcg ctggtcttgg aggacgccaa gctcggcgac tc
//
LOCUS       DATMKC010000465         4773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_134892, whole genome shotgun sequence.
ACCESSION   DATMKC010000465 DATMKC010000000
VERSION     DATMKC010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134892"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VNU01_13450"
     CDS             <1..856
                     /locus_tag="VNU01_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33670.1"
                     /translation="SGGKGACPSGTDGDGDDTGDGGDTGDDDDTGDDGDTGDDGGTGG
                     ASDAGCPVAAGGGDTALAGAIRAAFPAPRIVVAQPTALGTFTMPAGCPDAQVTLASYA
                     LVDGEPTLSQHSTGLFDAGTGRLAVAVPDCAFTVSLATGPALPSGRYGARLLATAQGG
                     TGDCARSDVQGDDRAPVGGGGGDGDGTMPLPDVPGVPGVPAAPGVPGDGAGAPANGTE
                     ATGGAPAAAGPAVGAAGGAAAGIAAVPSSAAPGGTLPATGGSAMIVGLLTMLGALGLR
                     LAARRGGA"
     gene            1071..2624
                     /locus_tag="VNU01_13455"
     CDS             1071..2624
                     /locus_tag="VNU01_13455"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWH33671.1"
                     /translation="MVPALALSAAAAAAPAAADGGPYDQPLELVFPADARAGFGDDYH
                     AGRSGGRVHKATDLMGQKLWKLYAAADGEICHQTGVRDAMPSWGYSLTVCGADGREYR
                     YIHLNNDTPGTDDGLGGPEWAYAPGVRQGAPVTAGQWVGYMGDSGNAERTAPHLHFEI
                     HDSTVTDPYGDQRLNPYPSLMAARAAGRMPADPGAPALDGVLRVAGSDRVGTAVELVR
                     RLRTDAAHVVLTPAGAYADTIAAAPLARALGGVLLPTWSDRLDPRVAEELRRLRPAGV
                     TVVGTQEQLRPEVVAAAVAAAGLEPGAARRIAGVDRYATAAAVAEAVWALAPDAGHEA
                     VVAVGHHADERRAWPDALSASSLAAAWSRPVLFATREHLPPVTAAALAGARGVTLVGG
                     PGAISAEVERLLATFVDGSVRRVYGQDRYATSVAVADELVRAGKARLGWVWTATGSEW
                     ADAVTAGAEAGRSGNALVLVDGAGGAGDAVAGRWLWRHGGALHSVLVLGGDQAVSGAA
                     AARLAQRIT"
     gene            complement(2621..3115)
                     /locus_tag="VNU01_13460"
     CDS             complement(2621..3115)
                     /locus_tag="VNU01_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33672.1"
                     /translation="MITTSRSRAVAIAAAALLLALLTSACGDPPATDPGDDAIVPPGE
                     MPAQTAVDVTVDNGDGQASFRLVCDDAPRFEGEAGERDAAAACDALLQAKDRLVTLSD
                     PDREPEMCTEQYGGPETAAITGTVEGEQVDVAIDRTNGCGIGDWERLQPLLGEPTGVA
                     EPVS"
     gene            3219..4499
                     /locus_tag="VNU01_13465"
     CDS             3219..4499
                     /locus_tag="VNU01_13465"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWH33673.1"
                     /translation="MAERHVVIVGAGFGGLAAARRLARRDARGHVRVTLVDRRNHHTF
                     QPLLYQVATAGLVPQDVGHSVRAIVAGWPNVAFRLGEVRGADFTRRVLRLDDGELAFD
                     ELIVAAGAVTADFGVPGVAEHAFPLKDLGEAVALRDHILRRFEGAGAAGAAEGELTFV
                     VAGGGPTGVEVAGALRELVDHALRRDFPEVDMAAVRIVLVEQAGELLGAFPPASRDYT
                     ARTLRARGVEVRLGTAIAEARPDAVVLAGGERLPARTLVWAAGVRANPLAEALGLPTG
                     RGGRVAVDEHLRVPGLAHVYAVGDIAAMPDGHGGPAPQLAPAAIQAARHVARVILRGD
                     VPPFRYRDKGVMSTIGRNAAVARLPGGIRLRGRLGWLSWLGLHLVMLIGFRNRLSVLL
                     NWAWSYLTYDHAARVVFQPAEPRAAAQPSGSAGA"
     gene            4535..>4773
                     /locus_tag="VNU01_13470"
     CDS             4535..>4773
                     /locus_tag="VNU01_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33674.1"
                     /translation="MSAAARASAPADALTAHLAAQLGAWPPAGPLVITTSGARRRPAW
                     DGVVRKFAGVRTPEGAVLSVPQELLDAVRRVTAGAR"
BASE COUNT          501 a         1807 c         1901 g          564 t
ORIGIN      
        1 gtccggcggc aagggcgcct gccccagcgg cactgacggg gacggcgacg acaccggaga
       61 cggcggcgac accggagacg acgacgacac cggagacgac ggcgacaccg gagacgacgg
      121 cggcaccggc ggggccagcg atgccggctg ccccgtggcg gccggcggtg gtgacaccgc
      181 cttggccggc gcgatccgcg ccgctttccc cgcgccgcgc atcgtcgtcg cccagcccac
      241 ggccctcggg acgttcacca tgcccgcggg ctgccccgac gcgcaggtga cgctcgcgtc
      301 ctacgcgctc gtcgacggcg agccgacgct gtctcagcac agcaccggac tgttcgacgc
      361 cgggaccggc cgcctcgcgg tggccgtgcc cgactgcgcc ttcacggtga gcctcgccac
      421 gggtcccgcg ctcccgtccg gccggtacgg ggcccggctg ctggcgaccg cgcagggcgg
      481 caccggggac tgtgcgcgca gcgacgtcca gggtgacgac cgggcaccgg tcggcggcgg
      541 cggcggcgac ggcgacggca cgatgccgct gcctgacgtg cccggcgtgc ccggcgtgcc
      601 tgccgcgccg ggtgtgcccg gtgacggcgc gggagcgccc gcgaacggca cggaggcgac
      661 cggcggagcg cccgcggccg ccggccccgc cgtgggcgcg gccggcggag cggcggctgg
      721 gatcgcggcc gtcccctcgt ccgcggcccc cggcgggacc ctgcccgcga ccggcggctc
      781 cgcgatgatc gtcgggctgc tgaccatgct cggcgccctg ggcctgcggc tggcggctcg
      841 ccgcggcggc gcctgagcgc tgggaccgaa gggaacgcgc gggggtccgg ccggtccagg
      901 cccggacccc cgcggcggga ctagtgcggg cggcccattc gcgatcttga cgccgtgccc
      961 cgtcaagtca ccgcccggcg gctgccgaag cgggaagggc tgacgtcggc ttcccggaag
     1021 gtcccgcctt gctgcgctct cgatccgccc gcgcccgttt gctcgcgctg gtggtcccgg
     1081 cactggcgct gtccgccgcc gctgccgccg cgcccgccgc cgccgacggc ggcccctacg
     1141 accagccgct ggagctggtg ttccccgccg acgcgcgtgc cggcttcggc gacgactacc
     1201 acgccgggcg cagcggcggg cgcgtgcaca aggccaccga cctgatgggc cagaagctgt
     1261 ggaagctgta cgcggcggcg gacggtgaga tctgccacca gacgggcgtg cgcgacgcca
     1321 tgccgagctg gggctactcg ctcaccgtct gcggcgccga cgggcgcgag taccgctaca
     1381 tccacctcaa caacgacacg ccgggcaccg acgacggcct gggcggcccc gagtgggcct
     1441 acgcccccgg cgtgcgccag ggcgcgcccg tgacggccgg ccagtgggtg ggctacatgg
     1501 gcgactccgg caacgccgag cgcaccgccc cgcacctgca cttcgagatc cacgactcca
     1561 cggtgaccga cccctacggc gaccagcgcc tgaaccccta cccgtcgctg atggcggcgc
     1621 gcgcggccgg gcgcatgccc gccgacccgg gcgcaccggc gctggacggc gtgctgcgcg
     1681 tcgccggcag cgaccgcgtc ggcaccgccg tggagctggt gcggcgcctg cgcacggacg
     1741 ccgcccacgt ggtgctcacc cccgccggag cgtacgccga caccatcgcc gccgctcccc
     1801 tggcgcgcgc gctgggcggc gtgctgctgc ccacctggag cgaccgtctg gacccgcgcg
     1861 tcgcggagga gctgcggcgg ctgcggcccg ccggcgtgac ggtggtcggc acccaggaac
     1921 agctgcggcc cgaggtcgtg gcggcggcgg tggcggccgc cgggctggag ccgggcgccg
     1981 cgcggcgcat cgccggggtg gaccgctacg ccacggcggc cgcggtcgcc gaggcggtgt
     2041 gggcgctggc gccggatgcg gggcatgagg cggtggtggc ggtcggccac cacgccgacg
     2101 agcgcagggc ctggccggac gcgctcagcg cgtcgtcgct ggcggcggcg tggtcgcgac
     2161 cggtgctgtt cgcgacgcgg gagcacctgc cgcccgtgac ggccgcggcg ctggccggcg
     2221 cgcgcggggt gacgctggtc ggcgggccgg gcgcgatctc ggcggaggtc gagcggctgc
     2281 tggccacgtt cgtggacggg agcgtgcggc gggtctacgg ccaggaccgc tacgcgacct
     2341 cggtggcggt ggccgacgag ctggtgcgcg ccggcaaggc gcgcctgggc tgggtgtgga
     2401 ccgcgaccgg gtccgagtgg gccgacgcgg tgacggcggg cgccgaggcc ggccgcagcg
     2461 gcaacgcgct ggtgctggtg gatggggccg gcggcgcggg cgacgccgtc gccggccgct
     2521 ggctgtggcg ccacggcggc gcgctgcact cggtcctggt gctgggcggc gaccaggccg
     2581 tgtccggcgc cgccgccgcc cgcctcgccc agcgcatcac ctaggagacg ggctcggcca
     2641 cgccggtggg ctcgcccagc agcggctgga ggcgctccca gtcgccgatg ccgcagccgt
     2701 tggtgcggtc gatggcgacg tcgacctgct cgccctcgac ggtgcccgtg atcgcggccg
     2761 tctccgggcc gccgtactgc tcggtgcaca tctccggttc ccggtccgga tcgctcagcg
     2821 tcaccaggcg gtccttggcc tggagcaggg cgtcgcacgc cgcggcggcg tcgcgctcac
     2881 ccgcctcgcc ctcgaagcgg ggcgcgtcgt cgcagaccag gcggaagctc gcctgcccgt
     2941 cgccgttgtc cacggtcacg tcgacggcgg tctgggccgg catctcaccc ggggggacga
     3001 tcgcgtcgtc accggggtcg gtcgccggcg ggtccccgca cgcggacgtc agcagcgcga
     3061 gcagcagggc cgcggcggcg atcgccaccg cgcgggagcg ggaggtcgtg atcatggccg
     3121 ttggacgcta ccggcccggc cggagttccg ggaatgcgcg gagggcttcc ggcgttcacg
     3181 attgtgaaaa ttttcacaat cgtggaagga gcagaggaat ggcggagcgg cacgtcgtca
     3241 tcgtgggcgc cgggttcggc ggcctcgccg cggccaggcg cctcgcgcgc cgcgacgcac
     3301 gcggccacgt ccgcgtgacg ctggtcgacc ggcgcaacca ccacaccttc cagccgttgc
     3361 tgtaccaggt cgccacggcc ggcctggtcc cccaggacgt cggccacagc gtgcgcgcca
     3421 tcgtggccgg ctggccgaac gtggcgttcc ggctgggcga ggtgcgtggc gccgacttca
     3481 cgcggcgtgt cctgcgactg gacgacggcg agctggcctt cgacgagctg atcgtggccg
     3541 cgggcgcggt gacggcggac ttcggcgtgc cgggggtcgc cgagcacgcg ttcccgctga
     3601 aggacctcgg cgaggcggtg gccctgcgcg accacatcct gcgccgcttc gagggcgccg
     3661 gggcggcggg cgccgccgag ggcgagctca cgttcgtggt cgcgggcggc gggcccaccg
     3721 gcgtggaggt ggcgggcgcc ctgcgggagc tggtggacca cgcgctgcgc cgggacttcc
     3781 cggaggtcga catggccgcg gtgcgcatcg tcctggtcga gcaggccggg gagctgctgg
     3841 gcgcgttccc gccggcgtcg cgcgactaca ccgcccgcac gctgcgcgcg cggggcgtcg
     3901 aggtgcgcct gggcaccgcg atcgccgagg cgcggccgga cgcggtggtg ctggccggcg
     3961 gtgagcggct gcccgcgcgc accctggtgt gggcggccgg cgtgcgcgcg aaccccctcg
     4021 ccgaggcact cggcctgccg accggccggg gcggccgcgt cgcggtcgac gagcacctgc
     4081 gggtgccggg cctggcgcac gtgtacgccg tcggcgacat cgcggcgatg ccggacgggc
     4141 acggcgggcc ggccccgcag ctcgcgcccg cggcgatcca ggcggcacga cacgtcgccc
     4201 gtgtgatcct gcgcggcgac gtcccgccgt tccgctaccg cgacaagggc gtcatgtcga
     4261 ccatcgggcg caacgcggcc gtggcgcggc tgcccggcgg catccggctg cgcggccgtc
     4321 tcggctggct gtcctggctg gggctgcacc tggtcatgct gatcggcttc cgcaaccgcc
     4381 tcagcgtgct gctgaactgg gcgtggagct acctcaccta cgaccacgcg gcgcgcgtcg
     4441 tgttccagcc ggcggagccg cgcgcggccg cgcagccgtc cggctccgcg ggggcgtgac
     4501 cgcgagactc gtggacatcc ctatcctggg cgcgatgtcc gccgccgctc gcgcctccgc
     4561 ccccgccgac gcgctcaccg cgcacctcgc cgcccagctg ggtgcatggc ccccggcggg
     4621 cccgctcgtg atcacgacca gcggcgcgcg ccggcgcccg gcctgggacg gcgtggtgcg
     4681 caagttcgcc ggggtgcgca ctccggaggg cgcggtgctg tccgtgccgc aggagctgct
     4741 cgacgccgtc cgccgcgtca ccgccggcgc gcg
//
LOCUS       DATMKC010000466         3711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_339920, whole genome shotgun sequence.
ACCESSION   DATMKC010000466 DATMKC010000000
VERSION     DATMKC010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339920"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..897)
                     /locus_tag="VNU01_13475"
     CDS             complement(301..897)
                     /locus_tag="VNU01_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33675.1"
                     /translation="MMNLRTIFSATAAATLLVVGPGWALAGPNGHLSGDTSGSAQGAA
                     QIAEGGIDGDAVAESTTRFDAEFDAGDMPEAPEAPQAPEAPELPEAPEAPQASAPEVE
                     QPEAPEVPQGEAPQGPEAPSAPEAPEVPQPGLPADGSAPKGEKPALDKPSTGDGSVRA
                     EGELHVSGSVRGGNPPEADSDADGRGSVEAAGQQVTTP"
     gene            complement(1040..1795)
                     /locus_tag="VNU01_13480"
     CDS             complement(1040..1795)
                     /locus_tag="VNU01_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWH33676.1"
                     /translation="MLLDKTVLVTGASSGIGEACARRFAAAGARLLLCARRAGRLEAL
                     AAELDTDVHAFALDVRDRDAVGAAVAALPEGWRDVDVLVTNAGLAAGFGPLPDNDPDD
                     WDRMLDTNVKGLLHVTRAVVPGMVARGGGHVINVGSVAGHETYPGGAVYCASKAAVDR
                     ITKGLRLDLLGSGVKVSTVDPGLVETEFSVVRFGGDADAAARVYHGMTPLTADDVAET
                     VVWVADRPAHVQVAEVLLFPADQAAATAVHRRR"
     gene            complement(1858..3270)
                     /locus_tag="VNU01_13485"
     CDS             complement(1858..3270)
                     /locus_tag="VNU01_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWH33677.1"
                     /translation="MADDELLFRPALDLAAAVRAREVSPVELARTALERIERLNGELN
                     AVVHVDADGALEAARAAERRVTAAEDDVPPFLGVPTLIKDVHPVAGMRTTFGTRALAE
                     FVPPFDAEHVRRLREAGFVFLGKTNVPEFGTVAQTEPELFGPARNPWDTSRTPGGSSG
                     GAAAALAAGMVPVADGSDGGGSTRIPAAACGVFGLKPARGRVSNGPLFGDLYFGLVTP
                     GPLTRTVADAAAMLDVMAGYAPGDPNWAPPPPRPFLDEARREPGCLRIGMLTESPLAH
                     RTVGAEAVAATEELGRLLESLGHRVEPCTLPIPEQTEELFTDVWTAGLASNPIPRELL
                     EPYNQRLAERGTAISGPKLIQSVSALQLIARGIVGASLAYDAIIYATLTHAPFRVGEL
                     AGLDPDEAYDRDATFVGFTPIANVTGQPAVSMPVGWSADGLPIGVSALGRPADEATLL
                     RLAAQVEAATGWPSRRPPVS"
     gene            3319..3684
                     /locus_tag="VNU01_13490"
     CDS             3319..3684
                     /locus_tag="VNU01_13490"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HWH33678.1"
                     /translation="MDVQMLGLAKSQATRKAQRFFSERRVPFHFNDLAKRPPTPGELR
                     KWVQRFGAQGCLDPESRSYTEQGLQWVSAGEDAWVERMVKDPSILRLPLVRCGKELSV
                     GDDPEAWARFAEAAKAAGG"
BASE COUNT          430 a         1358 c         1438 g          485 t
ORIGIN      
        1 ccccaagatg ctgctgatgg atgtgccccg tggaagctgt cggttttctc tccgtgtgct
       61 tcccccctcg gggctttgct gctgactcca agaatctcgg cggccgccgc ggccggctcc
      121 atcacccatt ccggtgacac cctcccgggg aagttggaag atcaggccca tatacgggcc
      181 tgatcttcca actcggggag ctgcgtgggt ggcgcgggag tggagcgggc ccccgccgca
      241 tccatgcgac gggggccctg gtgttctgtg gtggcgccgg cggcgcctgc cgggcgctgg
      301 ctacggcgtg gtgacctgct ggccggccgc ctcgacggag ccacggccgt cggcgtcgct
      361 gtcggcctcc ggcgggttgc cgccgcggac cgagccggac acgtgcagct cgccctcggc
      421 gcggaccgag ccgtcgccgg tgctgggctt gtccagcgcg ggcttctcac ccttcggcgc
      481 gctgccgtcc gcgggcagac ccggctgcgg gacctcggga gcctccggcg cgctgggcgc
      541 ctcggggccc tgcggggcct cgccctgcgg cacctcggga gcctccggct gctcgacctc
      601 gggggccgac gcctgcgggg cctcgggggc ctccggcagc tcgggagcct ccggcgcctg
      661 cggcgcctcg ggggcctccg gcatgtcgcc ggcgtcgaac tcggcgtcga agcgggtcgt
      721 gctctccgcc accgcgtcgc cgtcgatgcc gccctcggcg atctgcgcgg cgccctgggc
      781 gctgccgctg gtgtcaccgg acaggtggcc gttcgggccg gccagggccc agccggggcc
      841 gacgacgagc agggtcgcgg cggcggtggc gctgaagatc gtccgcaggt tcatcatggt
      901 gagtcctcct cggttgcgtc ccccggtcgt gggcccgggg cgttgggaga aggaacggcc
      961 acagtgacta gtccctgatt cgcccgttcg ggggacaagg actagtcccg tcgagtgatc
     1021 cccgcccgcg cgaagcggct cagcggcggc ggtgcacggc cgtggcggcc gcctggtcgg
     1081 ccgggaacag cagcacctcg gcgacctgca cgtgcgcggg ccggtccgcc acccacacca
     1141 ccgtctcggc gacgtcgtcg gccgtcagcg gcgtcatgcc gtggtagacc cgcgcggcgg
     1201 cgtcggcgtc gccgccgaag cgcaccacgg agaactcggt ctccaccagt ccggggtcca
     1261 ccgtcgacac cttcacgccg ctgcccagca ggtccaggcg cagccccttg gtgatgcggt
     1321 ccacggccgc cttggacgcg cagtacacgg cgccgcccgg gtaggtctcg tggccggcca
     1381 cggagccgac gttgatgacg tggccgccgc cgcgcgccac catgccgggc accaccgcgc
     1441 gggtgacgtg cagcaggccc ttcacgttgg tgtcgagcat gcggtcccag tcgtcggggt
     1501 cgttgtccgg cagcggcccg aagccggcgg ccaggccggc gttggtgacc agcacgtcga
     1561 cgtcccgcca gccttccggc agcgcggcga cggcggcgcc gaccgcgtcg cggtcgcgca
     1621 cgtccagcgc gaacgcgtgc acgtcggtgt ccagctcggc ggccagggcc tccaggcgtc
     1681 cggcccgccg ggcgcacagc agcaggcgcg cgcccgcggc ggcgaaccgg cgggcgcacg
     1741 cctcgccgat gccgctggac gctccggtca ccagcacggt cttgtcgagc agcatgggcc
     1801 ggtcctcagc gggtcggtgg gccagtgggt caacgggtcg gtgcggtggg cggccggtca
     1861 ggacaccggc gggcggcgcg acggccagcc ggtcgcggcc tcgacctggg cggccagccg
     1921 cagcagcgtc gcctcgtccg cggggcggcc cagcgcgctc acgccgatcg gcaggccgtc
     1981 cgccgaccag cccaccggca tcgacaccgc gggctggccg gtgacgttgg cgatgggcgt
     2041 gaagccgacg aacgtggcgt cgcggtcgta ggcctcgtcg gggtccaggc cggcgagctc
     2101 gccgacgcgg aacggcgcgt gggtcagcgt ggcgtagatg atcgcgtcgt aggccaggct
     2161 cgcgccgacg atgccgcggg cgatgagctg cagcgcgctc acggactgga tcagcttggg
     2221 gccgctgatc gcggtgccgc gctcggccag gcgctggttg tacggctcca gcagctcgcg
     2281 cgggatcggg ttggacgcca ggcccgccgt ccagacgtcg gtgaacagct cctcggtctg
     2341 ctcggggatc ggcagcgtgc acggctccac gcggtggccg agcgattcca gcagccggcc
     2401 cagctcctcg gtggcggcca cggcctccgc gccgacggtg cggtgcgcca gcggcgactc
     2461 ggtcagcatg ccgatgcgca ggcagccggg ctcccggcgc gcctcgtcca ggaacgggcg
     2521 cggcggcggc ggcgcccagt tcgggtcgcc gggcgcgtag cccgccatga cgtccagcat
     2581 cgcggctgcg tcggccacgg tgcgcgtgag cgggcccggg gtgaccaggc cgaagtacag
     2641 gtcgccgaac agcggcccgt tggacacgcg accgcgcgcg ggcttcaggc cgaacacgcc
     2701 gcacgcggcc gcggggatgc gcgtcgagcc gccgccgtcg gagccgtcgg cgacgggcac
     2761 catgccggcg gccagcgccg cggccgcgcc accggaggac ccgccgggcg tgcgcgaggt
     2821 gtcccacggg ttgcgcgcgg gaccgaacag ctccggctcg gtctgcgcga ccgtgccgaa
     2881 ctccggcacg ttggtcttgc ccaggaacac gaagccggcc tcgcgcaggc ggcgcacgtg
     2941 ctcggcgtcg aacggcggca cgaactccgc cagggcccgc gtgccgaacg tggtgcgcat
     3001 gccggcgacc gggtgcacgt ccttgatcag cgtcggcacg cccaggaacg gcggcacgtc
     3061 gtcctcggcg gcggtgacgc gccgctcggc ggcgcgggcg gcctccaggg cgccgtcggc
     3121 gtcgacgtgc accaccgcgt tgagctcgcc gttcaggcgc tcgatgcgct ccagcgcggt
     3181 ccgcgccagc tccaccggcg agacctccct ggcccgcacg gccgcggcca ggtccagcgc
     3241 ggggcggaac agcagctcgt cgtcggccac tggcgggtcc tttcggcgcg gggcgggcag
     3301 gcgcatagca tgccaacgat ggacgtccag atgctcgggc tggccaagtc gcaggccacg
     3361 cgcaaggcgc agcggttctt ctcggagcgc cgcgtgccgt tccatttcaa cgacctggcg
     3421 aagcggccgc cgacgccggg ggagctgcgc aagtgggtgc agcgcttcgg cgcgcaaggc
     3481 tgcctggacc cggagtcgcg ctcctacacc gagcagggcc tgcagtgggt gtccgcgggc
     3541 gaggacgcct gggtcgagcg gatggtgaag gacccgtcga tcctgcgcct gccgctggtg
     3601 cgctgcggca aggagctgtc ggtgggcgac gacccggagg cgtgggcccg cttcgccgag
     3661 gcggccaagg cggccggcgg gtgagccgcg tcacgctgct cgcggccgac c
//
LOCUS       DATMKC010000467         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_348196, whole genome shotgun sequence.
ACCESSION   DATMKC010000467 DATMKC010000000
VERSION     DATMKC010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348196"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VNU01_13495"
     CDS             complement(<1..231)
                     /locus_tag="VNU01_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33679.1"
                     /translation="MAAYTTLRASIRWALALLFALALLLAAALPAVAVAGGNPNPPGQ
                     GGEGGQGPGDDDCDDNEGSGNDDKCKSGDGDGD"
     gene            complement(324..1592)
                     /gene="hisS"
                     /locus_tag="VNU01_13500"
     CDS             complement(324..1592)
                     /gene="hisS"
                     /locus_tag="VNU01_13500"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWH33680.1"
                     /translation="MSADRVPQGVKGAPDWLPPDAAAFDFVESTFLGVARAAGYERVR
                     TPVFEHTEVFLRGVGGSTDIVQKEMYSFTDKGERSLTLRPEGTAGVMRAALQAGVPRS
                     GSLPAKWCYAGEFFRQERPQAGRQRQFNQVGIEALGSDDPLLDAEVVLLGWEALRQAG
                     VVNLTLRLNTLGDRGDRPAYLERLNAYLDGFDDLPPEVQERRRLNPLRAFDSKAPGMD
                     AVMAGAPVLADHVSGEAREHHRTVVALLEAEGVPLTHDARLVRGLDYYTRTTFEYQAG
                     NLGAQNAVGGGGRYDGLAEDLGWPERFPGIGWALGVDRTVLALREGGGLAQAPARVAA
                     FVVVTAPELRADGFALTARLRRAGVPADMAFDGRSVKAQFKAADRSGASRVVVVGPDE
                     AGRGAVTVKDLTTGVQQEVGREQLVEALRS"
     gene            complement(1795..2019)
                     /locus_tag="VNU01_13505"
     CDS             complement(1795..2019)
                     /locus_tag="VNU01_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWH33681.1"
                     /translation="MLMAALPSSDDDRPATRRDLAKEREILEARFGTVDAKFDAVRAE
                     IAGLRREMRILLGIVMVMLSGLVGLNLGAG"
BASE COUNT          304 a          813 c          715 g          300 t
ORIGIN      
        1 gtcgccgtcc ccatcgccgc tcttgcactt gtcgtcgttg ccggagccct cgttgtcgtc
       61 gcagtcgtcg tcacccggcc cctgaccacc ctcgccgccc tgaccgggag ggttggggtt
      121 gccccccgcg acggccacgg ccggcagtgc ggcggcgagc aggagcgcca gcgcaaacag
      181 cagcgcgagt gcccagcgga tggaggcgcg gagggtggtg taagcagcca tggccccacg
      241 gtgccttcgc accattgggg gcttaatagc gaatcgaggt caattcggtg tagtccgggt
      301 tgggggtccc gggaccatcc ggatcagcta cgaagcgcct cgacgagctg ttctcggccg
      361 acttcctgtt ggactcccgt agtcaggtcc ttcaccgtga ccgcgccccg cccggcctcg
      421 tcgggaccaa cgaccaccac ccgcgacgcg cccgagcggt ccgccgcctt gaactgcgcc
      481 ttcacgctgc gcccgtcgaa cgccatgtcc gcgggcacgc cggcccggcg cagccgcgcc
      541 gtgagcgcga acccgtcggc ccgcagctcc ggtgccgtga ccaccacgaa cgccgccacg
      601 cgcgccggcg cctgcgcgag cccgccgccc tcccgcaacg ccagcacggt gcggtccacg
      661 cccagcgccc agccgatgcc ggggaagcgc tccggccagc ccaggtcctc ggccaggccg
      721 tcgtagcggc cgccgccgcc cacggcgttc tgcgcgccga ggttgcccgc ctggtactcg
      781 aacgtcgtcc gcgtgtagta gtcgaggccg cgcaccaggc gcgcgtcgtg cgtcagcggc
      841 acgccctcgg cctccagcag cgccaccacc gtgcggtggt gctcgcgcgc ctcgccggag
      901 acgtggtcgg ccagcaccgg cgcgccggcc atgaccgcgt ccatgccggg cgccttggag
      961 tcgaacgccc gcaggggatt gagcctgcgg cgctcctgca cctccggcgg caggtcgtcg
     1021 aagccgtcga ggtaggcgtt caggcgctcc aggtaggccg gccggtcgcc gcggtcgccc
     1081 agcgtgttca acctcaacgt gaggttcacc acgcccgcct ggcgcagcgc ctcccagccg
     1141 agcagcacca cctcagcgtc aagcagaggg tcatccgagc ccagcgcctc gatgccgacc
     1201 tggttgaact ggcgctggcg gccggcctgc ggccgctctt ggcggaagaa ctcccccgcg
     1261 tagcaccact tggcgggcag cgagcccgac cgcggcaccc cggcctgcag cgcggcgcgc
     1321 atcacgccgg ccgtgccctc cgggcgcagc gtcagcgagc gctcgccctt gtcggtgaag
     1381 gagtacatct ccttctgcac gatgtcggtc gagccgccga cgccgcgcag gaacacctcg
     1441 gtgtgctcga acaccggcgt gcggacgcgc tcgtagccgg cggcgcgggc cacgccaagg
     1501 aacgtcgact ccacgaagtc gaacgcggcg gcgtcgggcg gcagccagtc gggcgcgccc
     1561 ttcacgccct gcggcacacg gtcggcggac atggtggcgc atgctagccg ctcggctcac
     1621 cagccacgga aacccctcgg cgtgcgcgcg aagtgacaac cacaaccggg tggtttcttg
     1681 acggttgttc gcttcagaac cgtcccgaat tgtccgacac tgatgtcagg cgacttgctc
     1741 agggacggac aagcgctcga tcgggggcca gggactggcc cctgatctca tttctcagcc
     1801 cgctcccagg ttcagcccga cgagcccgct cagcatcacc atcacgatgc cgagcaggat
     1861 gcgcatctca cggcgcagac cggcgatctc ggcgcgcacc gcgtcgaact tcgcgtcgac
     1921 ggtgccgaac ctcgcctcga gaatctcccg ctccttggcg agatcccggc gcgtggccgg
     1981 ccggtcgtcg tcggaggatg gcagcgcggc catcagcacg tcgcctcctg tcgcatgtcc
     2041 ccgcgtcgtc gcgcgcgacg gtagagcacc gagcccgctc gcgccatcga tgcggccgcg
     2101 gagctgtgga gaacttgcgg ctcctacagg aa
//
LOCUS       DATMKC010000468         3622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Egibacteraceae bacterium isolate SMAG_U13586
            k141_252903, whole genome shotgun sequence.
ACCESSION   DATMKC010000468 DATMKC010000000
VERSION     DATMKC010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942986
            Sequence Read Archive: SRR8438987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Egibacteraceae bacterium (soil metagenome)
  ORGANISM  Egibacteraceae bacterium
            Bacteria; Actinomycetota; Nitriliruptoria; Egibacterales;
            Egibacteraceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:33:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 42
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Egibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252903"
                     /isolate="SMAG_U13586"
                     /isolation_source="soil"
                     /db_xref="taxon:3047544"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="32.5788 N 110.8509 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /gene="thpR"
                     /locus_tag="VNU01_13510"
     CDS             <1..477
                     /gene="thpR"
                     /locus_tag="VNU01_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWH33682.1"
                     /translation="GLRRAHPGVRWTDPAGWHLTVVFLGWVPADRVDAVERALGRAAA
                     RVPPFTLALTGAAGSFRSGVAWAELEEQPALARLAAAAAGELGAVVELPGEGRPFRAH
                     LTLARAGRRPAEAREVAGSYRGPASRWDVERVVLMRSHLSKSGARYETVGAWPLGG"
     gene            595..2994
                     /gene="recA"
                     /locus_tag="VNU01_13515"
     CDS             595..2994
                     /gene="recA"
                     /locus_tag="VNU01_13515"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWH33683.1"
                     /translation="MREPDGTREQKGGVMEREKALDMALGQIEKQFGKGAIMRMGADD
                     HVQVAAIPTGALSLDLALGIGGLPRGRVVEIYGPESSGKCLTADTYVWTDRGLETVAE
                     LFERCGQPASCTSRVTDVRDQCVRAVNERGALEPIAALTHNNRKPVVHIQCQSGRVVK
                     VTHNHPLRVVSERGYIVWRKAGDIAVGDTVVSALFGAAEAATGEGLSEDEAVILGYLV
                     AEGSLSSTTAVRFTNWDLEVGGEFTRLMERLFDAEVRCYDGKEYAVHRKALRELFAER
                     YGLDYVTAAGKSVPYVIRTGGQKIQQAFLSALFEGDGWIDQSSTIGLGTASEELARQV
                     QLLLYGFGIPCTVASSFNVDYGRDYWTVTINPAVAHRFLEEVGFRSQRRAAQVEAAFR
                     QSPREPQFENIPNLAGLLSDLRDDICGDREFDRVAGDLFRKDIALRCSRRRLSAIIAW
                     ADERADRLSADGRAIVDHLRALAAARYTYETVVDVKAGGNEPTFDLMLPDTHSFLANG
                     MVSHNTTVALHAIAEAQKLGGIAAFIDAEHALDPTYAAALGVDVEALLVSQPDTGEQA
                     LEITDMLVRSGAIDIVVIDSVAALTPKAEIEGEMGDTHVGLQARLMSQALRKLSGNLN
                     KSRTCAVFINQIREKIGVMFGSPEVTPGGRALKFYASVRLDVRRIESLKDGQDFVGNR
                     TRIKVVKNKVSPPFKQAEFDILFGEGISKEGSIIDMGVEHGIVKKAGAWYTYEGEQLG
                     QGRENARSFLKEHEDTANEIYKRVTEVLGLVPTDVTGADTMPPDGAVEAAADDGFDDE
                     A"
     gene            2991..3494
                     /locus_tag="VNU01_13520"
     CDS             2991..3494
                     /locus_tag="VNU01_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HWH33684.1"
                     /translation="MSAPQVTAEGEAGKALAFLLRSTSARPQTEAELRAKLRAREVPD
                     EVADEALAKARALGAVDDRAFAFAWVEDRGRVRGYGASRLRQELRRRLVPDAILDEAL
                     ASLEDRDEEDAALELARRRAQRMPADLPPETVARRVMGFLVRRGYPPGVAQRAVRKAT
                     SLDREWD"
BASE COUNT          557 a         1182 c         1353 g          530 t
ORIGIN      
        1 ggcctgcgcc gggcgcaccc gggggtgcgc tggaccgacc ccgccggctg gcacctgacc
       61 gtggtgttcc tgggctgggt gccggccgac cgcgtcgacg cggtggagcg ggcgctgggg
      121 cgcgcggccg cccgcgtgcc gccgttcacg ctggccctga ccggcgcggc cggcagcttc
      181 cgctccggcg tggcgtgggc ggagctggag gagcagcccg cgctcgcgcg gctggcggcg
      241 gcggccgccg gggagctcgg cgcggtggtg gagctgccgg gggaggggcg gccgttccgc
      301 gcgcacctca cgctggcgcg ggccggccgg cgcccggccg aggctcgtga ggtggccggc
      361 tcctaccggg gtccggcgtc gcgctgggac gtggagcgcg tggtgctgat gcgctcccac
      421 ctgtccaagt ccggcgcgcg ctacgagacc gttggggcct ggccgctggg cggctgaggg
      481 ccctgcgcgc gagatgcgtg acgggcgaac gcatgttcgg tagcatgcgt ccacagccga
      541 gtggcacgga ttccgggcgc tgtcacagcg ccggactagg gtggccacac cacggtgcgc
      601 gaaccggacg gcacgcgcga gcagaaaggt ggcgtgatgg agcgcgagaa agcgctggac
      661 atggccctcg gccagatcga gaagcagttc ggcaagggcg cgatcatgcg catgggcgcc
      721 gacgaccacg tgcaggtcgc ggcgatcccc acgggcgcgc tgagcctcga tctcgcgctc
      781 ggcatcggcg gcctgccgcg cggccgcgtc gtggagatct acggaccgga gagcagcggc
      841 aagtgcctga ccgcagacac ctatgtgtgg acggatcgcg gcctcgagac ggtcgccgag
      901 ctgttcgagc gatgcggtca gccggcaagc tgtacgtcgc gcgtgaccga cgtgcgtgac
      961 cagtgtgtcc gggccgtgaa cgagcgtggc gcattggagc cgatcgccgc gctcacgcac
     1021 aacaaccgca agcccgtggt gcacatccaa tgccagtccg gccgcgtggt gaaggtcacg
     1081 cacaaccatc cgctccgtgt ggtcagcgaa cgcggctaca tcgtgtggcg gaaggctgga
     1141 gacatagcgg tcggtgacac ggtggtgtcg gccctctttg gcgcggctga ggcggcgacg
     1201 ggcgaagggc tgagcgaaga cgaggctgtg atcctgggat atctcgtggc cgaaggctcc
     1261 ctgtcgtcga ccacggccgt ccgcttcacc aactgggacc tcgaagtcgg cggtgagttc
     1321 accaggctca tggagcggtt gttcgatgcg gaagtccgct gctatgacgg caaggaatac
     1381 gccgtccacc gcaaggcatt gcgggagtta ttcgccgagc gttacgggct cgactatgtg
     1441 acagcggcag gcaagtcggt gccgtacgtg atccgcaccg gcgggcagaa gatccagcaa
     1501 gcgttcctgt cggcgctgtt cgaaggtgac ggatggattg accagagttc gacgatcggc
     1561 ttggggaccg cctccgagga gcttgcccgt caggtgcaac tcctgctcta tggattcggt
     1621 atcccgtgca ccgtggcttc ctcgttcaat gtcgactacg gccgcgacta ctggacggtg
     1681 acgatcaacc ccgccgtggc acaccgattc ctcgaggagg tcggttttcg ctcgcagcgc
     1741 cgagccgccc aggtcgaggc ggcgttccgc cagtcaccac gcgaaccgca gttcgagaac
     1801 atcccgaacc tcgctgggtt gttgagcgat ctgcgggacg acatctgcgg agatcgggag
     1861 ttcgaccgcg tcgcaggcga cctctttcgt aaggacatcg cactgcggtg ttcgcgccgc
     1921 cggctcagcg cgatcatcgc gtgggcagat gagcgagcgg accggctctc agccgatgga
     1981 cgtgcgatcg tcgaccacct gcgcgccctg gccgcggcgc gctacaccta cgagacggtg
     2041 gtggacgtca aggccggcgg aaacgagccg acgttcgatc tcatgctgcc tgacacgcac
     2101 agcttcctgg cgaacgggat ggtgtcgcac aacaccactg tggcgctgca cgccatcgcg
     2161 gaggcgcaga agctcggcgg gatcgcggcg ttcatcgacg ccgagcacgc gctggacccg
     2221 acctatgcgg ccgcgctggg cgtggacgtc gaggcactgc tcgtgtcgca gccggacacc
     2281 ggcgagcagg cgctggagat caccgacatg ctggtgcgct ccggggccat cgacatcgtg
     2341 gtcatcgact cggtcgcggc gctcacgccc aaggccgaga tcgagggcga gatgggcgac
     2401 acacacgtcg gcctgcaggc gcggctgatg agccaggcgc tgcgcaagct gtcgggcaac
     2461 ctcaacaagt cccgtacctg cgccgtcttc atcaaccaga tccgcgagaa gataggagtg
     2521 atgttcggtt ctcccgaagt tactcctggt gggcgcgcgc tgaagttcta cgcctcggtg
     2581 cggttggacg tgcgacgcat cgagtcgctc aaggacggcc aggacttcgt gggcaaccgc
     2641 acccgcatca aggtggtcaa gaacaaggtg agtcctcctt tcaaacaggc cgaattcgat
     2701 attttgttcg gtgagggcat ctccaaggag ggctcgatca tcgacatggg cgtcgagcac
     2761 ggcatcgtga agaaggccgg cgcctggtac acgtacgagg gcgagcagct ggggcagggc
     2821 cgcgagaacg cccggtcctt cctcaaggag cacgaggaca ccgccaacga gatctacaag
     2881 cgggtcaccg aggtgctcgg cctggtgccc accgacgtca ccggcgccga cacgatgccc
     2941 cctgacggag ccgttgaggc ggccgcggac gacgggttcg acgacgaggc gtgagcgccc
     3001 cgcaggtcac cgcggagggg gaggccggca aggcgctggc cttcctgctc cgctccacgt
     3061 cggcccggcc gcagaccgaa gccgagctgc gggccaagct gcgcgcccgc gaggtcccgg
     3121 acgaagtcgc cgacgaggcg ctcgccaagg cgcgggcgct cggcgccgtg gacgaccggg
     3181 cgttcgcgtt cgcgtgggtg gaggaccgcg ggcgggtgcg cgggtatggc gcctcccgcc
     3241 tgcggcagga gctgcgccgc cgcctggtcc cggacgcgat cctcgacgag gcgctggcct
     3301 ccctggagga ccgcgacgag gaggacgccg ccctggagct cgcgcgccgg cgcgcgcagc
     3361 gcatgcccgc cgacctgccg ccggagacgg tggcgcggcg ggtcatgggc ttcctggtgc
     3421 gccggggcta cccgccgggg gtcgcccagc gcgccgtgcg caaggccacg tcgctggacc
     3481 gcgagtggga ctgaaaggcg cgcggacgcg caacggcccc gccgggtgac tggcggggcc
     3541 gttcgctggg ggacttgccg tgccgtccgg ggcgacggtg cctctacgcg ttcgccctgg
     3601 tgagcctgcc gtcggcgtag gc
//
